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Database: PDB
Entry: 5EFM
LinkDB: 5EFM
Original site: 5EFM 
HEADER    APOPTOSIS                               23-OCT-15   5EFM              
TITLE     BECLIN 1 FLEXIBLE-HELICAL DOMIAN (FHD) (141-171)                      
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: BECLIN-1;                                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: COILED-COIL MYOSIN-LIKE BCL2-INTERACTING PROTEIN,PROTEIN    
COMPND   5 GT197;                                                               
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   4 ORGANISM_COMMON: HUMAN;                                              
SOURCE   5 ORGANISM_TAXID: 9606                                                 
KEYWDS    FLEXIBLE HELIX, APOPTOSIS                                             
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    S.SINHA,Y.MEI                                                         
REVDAT   2   22-NOV-17 5EFM    1       JRNL   REMARK                            
REVDAT   1   20-JUL-16 5EFM    0                                                
JRNL        AUTH   Y.MEI,A.RAMANATHAN,K.GLOVER,C.STANLEY,R.SANISHVILI,          
JRNL        AUTH 2 S.CHAKRAVARTHY,Z.YANG,C.L.COLBERT,S.C.SINHA                  
JRNL        TITL   CONFORMATIONAL FLEXIBILITY ENABLES THE FUNCTION OF A BECN1   
JRNL        TITL 2 REGION ESSENTIAL FOR STARVATION-MEDIATED AUTOPHAGY.          
JRNL        REF    BIOCHEMISTRY                  V.  55  1945 2016              
JRNL        REFN                   ISSN 0006-2960                               
JRNL        PMID   26937551                                                     
JRNL        DOI    10.1021/ACS.BIOCHEM.5B01264                                  
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.95 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX                                               
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.95                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 44.66                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0                          
REMARK   3   NUMBER OF REFLECTIONS             : 3490                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.212                           
REMARK   3   R VALUE            (WORKING SET) : 0.212                           
REMARK   3   FREE R VALUE                     : 0.216                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.840                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 169                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1  0.0000 -  1.9500    1.00     3321   169  0.2116 0.2164        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.040            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 32.450           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 54.53                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.009            147                                  
REMARK   3   ANGLE     :  1.298            196                                  
REMARK   3   CHIRALITY :  0.068             19                                  
REMARK   3   PLANARITY :  0.004             27                                  
REMARK   3   DIHEDRAL  : 13.696             61                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5EFM COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 26-OCT-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000214787.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 26-JUN-12                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.6                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 23-ID-D                            
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.3776                             
REMARK 200  MONOCHROMATOR                  : SI (111)                           
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE                        
REMARK 200  DETECTOR MANUFACTURER          : MAR SCANNER 300 MM PLATE           
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS, XSCALE                        
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 3490                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.950                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 44.660                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 75.50                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.16000                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 26.3000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.95                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.05                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 78.10                              
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.800                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SAD                          
REMARK 200 SOFTWARE USED: SHELXD                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 56.82                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.85                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 250MM POTASSIUM SODIUM TARTRATE          
REMARK 280  TETRAHYDRATE, 2.2 M AMMONIUM SULFATE, 100MM SODIUM CITRATE          
REMARK 280  TRIBASIC DIHYDRATE (PH 5.6), EVAPORATION, TEMPERATURE 293K          
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 3 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290       5555   Z,X,Y                                                   
REMARK 290       6555   Z+1/2,-X+1/2,-Y                                         
REMARK 290       7555   -Z+1/2,-X,Y+1/2                                         
REMARK 290       8555   -Z,X+1/2,-Y+1/2                                         
REMARK 290       9555   Y,Z,X                                                   
REMARK 290      10555   -Y,Z+1/2,-X+1/2                                         
REMARK 290      11555   Y+1/2,-Z+1/2,-X                                         
REMARK 290      12555   -Y+1/2,-Z,X+1/2                                         
REMARK 290      13555   Y+1/4,X+3/4,-Z+3/4                                      
REMARK 290      14555   -Y+1/4,-X+1/4,-Z+1/4                                    
REMARK 290      15555   Y+3/4,-X+3/4,Z+1/4                                      
REMARK 290      16555   -Y+3/4,X+1/4,Z+3/4                                      
REMARK 290      17555   X+1/4,Z+3/4,-Y+3/4                                      
REMARK 290      18555   -X+3/4,Z+1/4,Y+3/4                                      
REMARK 290      19555   -X+1/4,-Z+1/4,-Y+1/4                                    
REMARK 290      20555   X+3/4,-Z+3/4,Y+1/4                                      
REMARK 290      21555   Z+1/4,Y+3/4,-X+3/4                                      
REMARK 290      22555   Z+3/4,-Y+3/4,X+1/4                                      
REMARK 290      23555   -Z+3/4,Y+1/4,X+3/4                                      
REMARK 290      24555   -Z+1/4,-Y+1/4,-X+1/4                                    
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       31.58150            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       31.58150            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       31.58150            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       31.58150            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       31.58150            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       31.58150            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY2   5  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY1   6  0.000000  0.000000  1.000000       31.58150            
REMARK 290   SMTRY2   6 -1.000000  0.000000  0.000000       31.58150            
REMARK 290   SMTRY3   6  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY1   7  0.000000  0.000000 -1.000000       31.58150            
REMARK 290   SMTRY2   7 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  1.000000  0.000000       31.58150            
REMARK 290   SMTRY1   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY2   8  1.000000  0.000000  0.000000       31.58150            
REMARK 290   SMTRY3   8  0.000000 -1.000000  0.000000       31.58150            
REMARK 290   SMTRY1   9  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   9  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY3   9  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY1  10  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2  10  0.000000  0.000000  1.000000       31.58150            
REMARK 290   SMTRY3  10 -1.000000  0.000000  0.000000       31.58150            
REMARK 290   SMTRY1  11  0.000000  1.000000  0.000000       31.58150            
REMARK 290   SMTRY2  11  0.000000  0.000000 -1.000000       31.58150            
REMARK 290   SMTRY3  11 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY1  12  0.000000 -1.000000  0.000000       31.58150            
REMARK 290   SMTRY2  12  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY3  12  1.000000  0.000000  0.000000       31.58150            
REMARK 290   SMTRY1  13  0.000000  1.000000  0.000000       15.79075            
REMARK 290   SMTRY2  13  1.000000  0.000000  0.000000       47.37225            
REMARK 290   SMTRY3  13  0.000000  0.000000 -1.000000       47.37225            
REMARK 290   SMTRY1  14  0.000000 -1.000000  0.000000       15.79075            
REMARK 290   SMTRY2  14 -1.000000  0.000000  0.000000       15.79075            
REMARK 290   SMTRY3  14  0.000000  0.000000 -1.000000       15.79075            
REMARK 290   SMTRY1  15  0.000000  1.000000  0.000000       47.37225            
REMARK 290   SMTRY2  15 -1.000000  0.000000  0.000000       47.37225            
REMARK 290   SMTRY3  15  0.000000  0.000000  1.000000       15.79075            
REMARK 290   SMTRY1  16  0.000000 -1.000000  0.000000       47.37225            
REMARK 290   SMTRY2  16  1.000000  0.000000  0.000000       15.79075            
REMARK 290   SMTRY3  16  0.000000  0.000000  1.000000       47.37225            
REMARK 290   SMTRY1  17  1.000000  0.000000  0.000000       15.79075            
REMARK 290   SMTRY2  17  0.000000  0.000000  1.000000       47.37225            
REMARK 290   SMTRY3  17  0.000000 -1.000000  0.000000       47.37225            
REMARK 290   SMTRY1  18 -1.000000  0.000000  0.000000       47.37225            
REMARK 290   SMTRY2  18  0.000000  0.000000  1.000000       15.79075            
REMARK 290   SMTRY3  18  0.000000  1.000000  0.000000       47.37225            
REMARK 290   SMTRY1  19 -1.000000  0.000000  0.000000       15.79075            
REMARK 290   SMTRY2  19  0.000000  0.000000 -1.000000       15.79075            
REMARK 290   SMTRY3  19  0.000000 -1.000000  0.000000       15.79075            
REMARK 290   SMTRY1  20  1.000000  0.000000  0.000000       47.37225            
REMARK 290   SMTRY2  20  0.000000  0.000000 -1.000000       47.37225            
REMARK 290   SMTRY3  20  0.000000  1.000000  0.000000       15.79075            
REMARK 290   SMTRY1  21  0.000000  0.000000  1.000000       15.79075            
REMARK 290   SMTRY2  21  0.000000  1.000000  0.000000       47.37225            
REMARK 290   SMTRY3  21 -1.000000  0.000000  0.000000       47.37225            
REMARK 290   SMTRY1  22  0.000000  0.000000  1.000000       47.37225            
REMARK 290   SMTRY2  22  0.000000 -1.000000  0.000000       47.37225            
REMARK 290   SMTRY3  22  1.000000  0.000000  0.000000       15.79075            
REMARK 290   SMTRY1  23  0.000000  0.000000 -1.000000       47.37225            
REMARK 290   SMTRY2  23  0.000000  1.000000  0.000000       15.79075            
REMARK 290   SMTRY3  23  1.000000  0.000000  0.000000       47.37225            
REMARK 290   SMTRY1  24  0.000000  0.000000 -1.000000       15.79075            
REMARK 290   SMTRY2  24  0.000000 -1.000000  0.000000       15.79075            
REMARK 290   SMTRY3  24 -1.000000  0.000000  0.000000       15.79075            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2470 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 3530 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -77.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2  0.000000  0.000000  1.000000       31.58150            
REMARK 350   BIOMT2   2 -1.000000  0.000000  0.000000       94.74450            
REMARK 350   BIOMT3   2  0.000000 -1.000000  0.000000       63.16300            
REMARK 350   BIOMT1   3  0.000000 -1.000000  0.000000       94.74450            
REMARK 350   BIOMT2   3  0.000000  0.000000 -1.000000       63.16300            
REMARK 350   BIOMT3   3  1.000000  0.000000  0.000000      -31.58150            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375 S    SO4 A 201  LIES ON A SPECIAL POSITION.                          
REMARK 375 O3   SO4 A 201  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH A 306  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     THR A   141                                                      
REMARK 465     ASP A   142                                                      
REMARK 465     THR A   143                                                      
REMARK 465     LEU A   144                                                      
REMARK 465     LEU A   145                                                      
REMARK 465     ASP A   146                                                      
REMARK 465     GLN A   147                                                      
REMARK 465     LEU A   148                                                      
REMARK 465     ASP A   149                                                      
REMARK 465     THR A   150                                                      
REMARK 465     GLN A   151                                                      
REMARK 465     LEU A   152                                                      
REMARK 465     ASN A   153                                                      
REMARK 465     VAL A   154                                                      
REMARK 465     THR A   155                                                      
REMARK 465     GLU A   156                                                      
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 201                 
DBREF  5EFM A  141   171  UNP    Q14457   BECN1_HUMAN    141    171             
SEQRES   1 A   31  THR ASP THR LEU LEU ASP GLN LEU ASP THR GLN LEU ASN          
SEQRES   2 A   31  VAL THR GLU ASN GLU CYS GLN ASN TYR LYS ARG CYS LEU          
SEQRES   3 A   31  GLU ILE LEU GLU GLN                                          
HET    SO4  A 201       5                                                       
HETNAM     SO4 SULFATE ION                                                      
FORMUL   2  SO4    O4 S 2-                                                      
FORMUL   3  HOH   *8(H2 O)                                                      
HELIX    1 AA1 CYS A  159  GLU A  170  1                                  12    
SSBOND   1 CYS A  159    CYS A  165                          1555   6566  2.06  
SITE     1 AC1  2 ASN A 161  TYR A 162                                          
CRYST1   63.163   63.163   63.163  90.00  90.00  90.00 P 43 3 2     24          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.015832  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.015832  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.015832        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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