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Database: PDB
Entry: 5EG0
LinkDB: 5EG0
Original site: 5EG0 
HEADER    TRANSCRIPTION                           26-OCT-15   5EG0              
TITLE     HOXB13-MEIS1 HETERODIMER BOUND TO DNA                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: HOMEOBOX PROTEIN MEIS2;                                    
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 284-338;                                      
COMPND   5 SYNONYM: MEIS1-RELATED PROTEIN 1;                                    
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: DNA (5'-                                                   
COMPND   9 D(P*GP*TP*TP*GP*AP*CP*AP*GP*TP*TP*TP*TP*AP*CP*GP*AP*GP*G)-3');       
COMPND  10 CHAIN: D;                                                            
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MOL_ID: 3;                                                           
COMPND  13 MOLECULE: DNA (5'-                                                   
COMPND  14 D(*CP*CP*TP*CP*GP*TP*AP*AP*AP*AP*CP*TP*GP*TP*CP*AP*AP*C)-3');        
COMPND  15 CHAIN: E;                                                            
COMPND  16 ENGINEERED: YES;                                                     
COMPND  17 MOL_ID: 4;                                                           
COMPND  18 MOLECULE: HOMEOBOX PROTEIN HOX-B13;                                  
COMPND  19 CHAIN: B;                                                            
COMPND  20 FRAGMENT: UNP RESIDUES  217-277;                                     
COMPND  21 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: MEIS2, MRG1;                                                   
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_VARIANT: ROSETTA;                                  
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PETG20A;                                  
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 SYNTHETIC: YES;                                                      
SOURCE  13 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE  14 ORGANISM_TAXID: 32630;                                               
SOURCE  15 MOL_ID: 3;                                                           
SOURCE  16 SYNTHETIC: YES;                                                      
SOURCE  17 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE  18 ORGANISM_TAXID: 32630;                                               
SOURCE  19 MOL_ID: 4;                                                           
SOURCE  20 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  21 ORGANISM_COMMON: HUMAN;                                              
SOURCE  22 ORGANISM_TAXID: 9606;                                                
SOURCE  23 GENE: HOXB13;                                                        
SOURCE  24 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  25 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  26 EXPRESSION_SYSTEM_VARIANT: ROSETTA;                                  
SOURCE  27 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  28 EXPRESSION_SYSTEM_PLASMID: PETG20A                                   
KEYWDS    TRANSCRIPTION FACTOR, HETERODIMER, COMPLEX, BOUND TO DNA,             
KEYWDS   2 TRANSCRIPTION                                                        
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    E.MORGUNOVA,Y.YIN,A.JOLMA,A.POPOV,J.TAIPALE                           
REVDAT   1   09-NOV-16 5EG0    0                                                
JRNL        AUTH   E.MORGUNOVA,Y.YIN,A.JOLMA,A.POPOV,J.TAIPALE                  
JRNL        TITL   MOLECULAR BASIS OF RECOGNITION OF TWO DISTINCT DNA SEQUENCES 
JRNL        TITL 2 BY A SINGLE TRANSCRIPTION FACTOR                             
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.10 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.9_1692                                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.10                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.95                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 9326                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.288                           
REMARK   3   R VALUE            (WORKING SET) : 0.286                           
REMARK   3   FREE R VALUE                     : 0.322                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.200                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 485                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 19.9462 -  4.4606    0.99     2992   155  0.2119 0.2660        
REMARK   3     2  4.4606 -  3.5470    0.99     2925   180  0.3341 0.3583        
REMARK   3     3  3.5470 -  3.1005    0.97     2924   150  0.3906 0.3882        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.380            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 37.340           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           1813                                  
REMARK   3   ANGLE     :  0.827           2593                                  
REMARK   3   CHIRALITY :  0.036            288                                  
REMARK   3   PLANARITY :  0.004            203                                  
REMARK   3   DIHEDRAL  : 26.294            746                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 279 THROUGH 334 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  14.0795 -12.1021 -20.6674              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3858 T22:   0.5089                                     
REMARK   3      T33:   0.3786 T12:  -0.0544                                     
REMARK   3      T13:  -0.0267 T23:   0.1471                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0763 L22:   0.0638                                     
REMARK   3      L33:   0.0238 L12:   0.0049                                     
REMARK   3      L13:  -0.0068 L23:   0.0109                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0770 S12:   0.1860 S13:   0.0979                       
REMARK   3      S21:  -0.0347 S22:   0.1778 S23:   0.1987                       
REMARK   3      S31:   0.0569 S32:  -0.1805 S33:   0.0319                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 20 THROUGH 37)                    
REMARK   3    ORIGIN FOR THE GROUP (A):   7.6850  -0.9248 -33.0833              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6637 T22:   1.2857                                     
REMARK   3      T33:   0.3175 T12:   0.2181                                     
REMARK   3      T13:   0.0106 T23:  -0.0201                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2160 L22:   1.0897                                     
REMARK   3      L33:   0.3355 L12:   0.4887                                     
REMARK   3      L13:  -0.1665 L23:  -0.4175                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2497 S12:   0.1379 S13:   0.3671                       
REMARK   3      S21:  -0.0643 S22:   0.6856 S23:  -0.0754                       
REMARK   3      S31:  -0.0145 S32:  -0.2310 S33:   0.2253                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 1 THROUGH 18)                     
REMARK   3    ORIGIN FOR THE GROUP (A):   7.2367   1.4291 -32.5406              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1000 T22:   0.8026                                     
REMARK   3      T33:   0.2824 T12:  -0.0763                                     
REMARK   3      T13:   0.0322 T23:   0.1670                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1429 L22:   0.0787                                     
REMARK   3      L33:   0.0806 L12:  -0.1180                                     
REMARK   3      L13:   0.0169 L23:  -0.0176                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0880 S12:   0.0615 S13:   0.2450                       
REMARK   3      S21:  -0.5436 S22:   0.5549 S23:   0.0087                       
REMARK   3      S31:  -0.5145 S32:  -0.9147 S33:   0.0977                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 217 THROUGH 277 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  20.2611  -0.8486 -46.5463              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2204 T22:   0.5277                                     
REMARK   3      T33:   0.3567 T12:  -0.2602                                     
REMARK   3      T13:   0.0666 T23:  -0.1125                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2484 L22:   0.1916                                     
REMARK   3      L33:   1.2619 L12:   0.2190                                     
REMARK   3      L13:   0.0746 L23:   0.1256                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0953 S12:   0.0858 S13:   0.0938                       
REMARK   3      S21:  -0.1269 S22:   0.3442 S23:  -0.2372                       
REMARK   3      S31:   0.3513 S32:   0.1313 S33:   0.2357                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5EG0 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 26-OCT-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000214842.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 06-NOV-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 8                                  
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID29                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.972390                           
REMARK 200  MONOCHROMATOR                  : SI(111) AND SI (311)               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : PSI PILATUS 6M                     
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 6006                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.970                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 40.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.0                               
REMARK 200  DATA REDUNDANCY                : 4.900                              
REMARK 200  R MERGE                    (I) : 0.28000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 3.7000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.97                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.13                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 95.2                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.00                               
REMARK 200  R MERGE FOR SHELL          (I) : 2.77000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 0.400                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 4XRM                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 54.46                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.70                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: PEG 5000, POTASSIUM CHLORIDE,            
REMARK 280  MAGNESIUM CHLORIDE, PENTANOL, PH 8, VAPOR DIFFUSION, SITTING        
REMARK 280  DROP, TEMPERATURE 293K                                              
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       20.86300            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       57.57600            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       28.16600            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       57.57600            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       20.86300            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       28.16600            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 5330 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 12810 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -22.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, D, E, B                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ALA A   278                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     TYR B 223    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   C2    DA E     7     NH2  ARG B   220              1.85            
REMARK 500   NH1  ARG B   220     O    HOH B   301              1.97            
REMARK 500   O    THR B   261     O    HOH B   302              2.03            
REMARK 500   O4'   DC E     2     O    HOH E   101              2.17            
REMARK 500   O2    DC E     1     O    HOH E   101              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DT D  21   O4' -  C1' -  N1  ANGL. DEV. =   1.8 DEGREES          
REMARK 500     DT D  22   O4' -  C1' -  N1  ANGL. DEV. =   1.9 DEGREES          
REMARK 500     DG D  36   O4' -  C1' -  N9  ANGL. DEV. =   1.9 DEGREES          
REMARK 500     DC E   1   O4' -  C1' -  N1  ANGL. DEV. =   2.2 DEGREES          
REMARK 500     DT E   3   O4' -  C1' -  N1  ANGL. DEV. =   1.8 DEGREES          
REMARK 500     DA E  10   O4' -  C1' -  N9  ANGL. DEV. =   2.1 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A 281      -60.83   -103.16                                   
REMARK 500    THR A 312      -58.09   -144.81                                   
REMARK 500    ILE A 330      -61.15   -129.12                                   
REMARK 500    GLU B 232      -18.06     77.09                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  5EG0 A  280   334  UNP    O14770   MEIS2_HUMAN    284    338             
DBREF  5EG0 D   20    37  PDB    5EG0     5EG0            20     37             
DBREF  5EG0 E    1    18  PDB    5EG0     5EG0             1     18             
DBREF  5EG0 B  217   277  UNP    Q92826   HXB13_HUMAN    217    277             
SEQADV 5EG0 ALA A  278  UNP  O14770              EXPRESSION TAG                 
SEQADV 5EG0 ALA A  279  UNP  O14770              EXPRESSION TAG                 
SEQRES   1 A   57  ALA ALA PRO LYS VAL ALA THR ASN ILE MET ARG ALA TRP          
SEQRES   2 A   57  LEU PHE GLN HIS LEU THR HIS PRO TYR PRO SER GLU GLU          
SEQRES   3 A   57  GLN LYS LYS GLN LEU ALA GLN ASP THR GLY LEU THR ILE          
SEQRES   4 A   57  LEU GLN VAL ASN ASN TRP PHE ILE ASN ALA ARG ARG ARG          
SEQRES   5 A   57  ILE VAL GLN PRO MET                                          
SEQRES   1 D   18   DG  DT  DT  DG  DA  DC  DA  DG  DT  DT  DT  DT  DA          
SEQRES   2 D   18   DC  DG  DA  DG  DG                                          
SEQRES   1 E   18   DC  DC  DT  DC  DG  DT  DA  DA  DA  DA  DC  DT  DG          
SEQRES   2 E   18   DT  DC  DA  DA  DC                                          
SEQRES   1 B   61  ARG LYS LYS ARG ILE PRO TYR SER LYS GLY GLN LEU ARG          
SEQRES   2 B   61  GLU LEU GLU ARG GLU TYR ALA ALA ASN LYS PHE ILE THR          
SEQRES   3 B   61  LYS ASP LYS ARG ARG LYS ILE SER ALA ALA THR SER LEU          
SEQRES   4 B   61  SER GLU ARG GLN ILE THR ILE TRP PHE GLN ASN ARG ARG          
SEQRES   5 B   61  VAL LYS GLU LYS LYS VAL LEU ALA LYS                          
FORMUL   5  HOH   *29(H2 O)                                                     
HELIX    1 AA1 LYS A  281  HIS A  294  1                                  14    
HELIX    2 AA2 SER A  301  ASP A  311  1                                  11    
HELIX    3 AA3 THR A  315  ILE A  330  1                                  16    
HELIX    4 AA4 GLY B  226  LEU B  231  1                                   6    
HELIX    5 AA5 ARG B  233  ASN B  238  1                                   6    
HELIX    6 AA6 ASP B  244  SER B  254  1                                  11    
HELIX    7 AA7 SER B  256  VAL B  274  1                                  19    
CISPEP   1 ALA A  279    PRO A  280          0        -4.81                     
CISPEP   2 ALA B  276    LYS B  277          0       -17.23                     
CRYST1   41.726   56.332  115.152  90.00  90.00  90.00 P 21 21 21    4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.023966  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.017752  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.008684        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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