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Entry: 5KNM
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HEADER    PROTEIN BINDING                         28-JUN-16   5KNM              
TITLE     HUMAN LEUKOCYTE ANTIGEN F (HLA-F) PRESENTS PEPTIDES AND REGULATES     
TITLE    2 IMMUNITY THROUGH INTERACTIONS WITH NK-CELL RECEPTORS                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CDNA FLJ39643 FIS, CLONE SMINT2004023, HIGHLY SIMILAR TO   
COMPND   3 HLA CLASS I HISTOCOMPATIBILITY ANTIGEN, ALPHACHAIN F;                
COMPND   4 CHAIN: A;                                                            
COMPND   5 FRAGMENT: UNP RESIDUES 22-305;                                       
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: BETA-2-MICROGLOBULIN;                                      
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: UNP RRSIDUES 21-119;                                       
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MOL_ID: 3;                                                           
COMPND  13 MOLECULE: LEUKOCYTE IMMUNOGLOBULIN-LIKE RECEPTOR SUBFAMILY B MEMBER  
COMPND  14 1;                                                                   
COMPND  15 CHAIN: D;                                                            
COMPND  16 FRAGMENT: UNP RESIDUES 24-221;                                       
COMPND  17 SYNONYM: LEUKOCYTE IMMUNOGLOBULIN-LIKE RECEPTOR 1,CD85 ANTIGEN-LIKE  
COMPND  18 FAMILY MEMBER J,IMMUNOGLOBULIN-LIKE TRANSCRIPT 2,ILT-2,              
COMPND  19 MONOCYTE/MACROPHAGE IMMUNOGLOBULIN-LIKE RECEPTOR 7,MIR-7;            
COMPND  20 ENGINEERED: YES;                                                     
COMPND  21 MOL_ID: 4;                                                           
COMPND  22 MOLECULE: PEPTIDE ILE-LEU-ARG-TRP-GLU-GLN;                           
COMPND  23 CHAIN: N;                                                            
COMPND  24 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE   7 EXPRESSION_SYSTEM_CELL: HIGH FIVE;                                   
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PACGP67A;                                 
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 GENE: B2M, CDABP0092, HDCMA22P;                                      
SOURCE  14 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  16 EXPRESSION_SYSTEM_PLASMID: PACGP67A;                                 
SOURCE  17 MOL_ID: 3;                                                           
SOURCE  18 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  19 ORGANISM_COMMON: HUMAN;                                              
SOURCE  20 ORGANISM_TAXID: 9606;                                                
SOURCE  21 GENE: LILRB1, ILT2, LIR1, MIR7;                                      
SOURCE  22 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  23 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  24 MOL_ID: 4;                                                           
SOURCE  25 ORGANISM_SCIENTIFIC: TRICHOPLUSIA NI;                                
SOURCE  26 ORGANISM_TAXID: 7111;                                                
SOURCE  27 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  28 EXPRESSION_SYSTEM_TAXID: 7111                                        
KEYWDS    MHC-IB, IMMUNE SYSTEM, PEPTIDE BINDING PROTEIN, PROTEIN BINDING       
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    C.L.DULBERGER,E.J.ADAMS                                               
REVDAT   3   27-SEP-17 5KNM    1       REMARK                                   
REVDAT   2   05-JUL-17 5KNM    1       JRNL                                     
REVDAT   1   14-JUN-17 5KNM    0                                                
JRNL        AUTH   C.L.DULBERGER,C.P.MCMURTREY,A.HOLZEMER,K.E.NEU,V.LIU,        
JRNL        AUTH 2 A.M.STEINBACH,W.F.GARCIA-BELTRAN,M.SULAK,B.JABRI,V.J.LYNCH,  
JRNL        AUTH 3 M.ALTFELD,W.H.HILDEBRAND,E.J.ADAMS                           
JRNL        TITL   HUMAN LEUKOCYTE ANTIGEN F PRESENTS PEPTIDES AND REGULATES    
JRNL        TITL 2 IMMUNITY THROUGH INTERACTIONS WITH NK CELL RECEPTORS.        
JRNL        REF    IMMUNITY                      V.  46  1018 2017              
JRNL        REFN                   ISSN 1097-4180                               
JRNL        PMID   28636952                                                     
JRNL        DOI    10.1016/J.IMMUNI.2017.06.002                                 
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.30 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.10_2155: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.30                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.41                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 17858                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.305                           
REMARK   3   R VALUE            (WORKING SET) : 0.303                           
REMARK   3   FREE R VALUE                     : 0.326                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.120                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 915                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 49.4119 -  6.3158    1.00     2619   130  0.2547 0.2852        
REMARK   3     2  6.3158 -  5.0146    1.00     2472   109  0.3005 0.3337        
REMARK   3     3  5.0146 -  4.3812    1.00     2384   152  0.2858 0.3275        
REMARK   3     4  4.3812 -  3.9808    1.00     2380   147  0.3300 0.3398        
REMARK   3     5  3.9808 -  3.6956    1.00     2377   129  0.3835 0.3916        
REMARK   3     6  3.6956 -  3.4778    1.00     2362   125  0.4013 0.4068        
REMARK   3     7  3.4778 -  3.3036    0.99     2349   123  0.4298 0.3717        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.560            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 39.490           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.003           4653                                  
REMARK   3   ANGLE     :  0.774           6337                                  
REMARK   3   CHIRALITY :  0.044            664                                  
REMARK   3   PLANARITY :  0.006            836                                  
REMARK   3   DIHEDRAL  :  8.025           3753                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 26                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 1 THROUGH 34 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -74.1984 136.9749 121.3148              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1937 T22:   1.4993                                     
REMARK   3      T33:   4.0996 T12:   0.0751                                     
REMARK   3      T13:   0.0787 T23:  -0.6252                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5979 L22:   6.2411                                     
REMARK   3      L33:   2.7260 L12:   2.4585                                     
REMARK   3      L13:   1.9567 L23:   2.7687                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6157 S12:  -1.1785 S13:   3.0290                       
REMARK   3      S21:   0.3759 S22:  -0.7580 S23:   1.7697                       
REMARK   3      S31:  -0.7270 S32:  -0.7679 S33:   0.2511                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 35 THROUGH 57 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -64.8460 134.0467 125.1442              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.2103 T22:   2.1009                                     
REMARK   3      T33:   2.2853 T12:  -0.3412                                     
REMARK   3      T13:  -0.0098 T23:  -1.0612                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.6639 L22:   4.5589                                     
REMARK   3      L33:   6.7692 L12:  -0.3047                                     
REMARK   3      L13:  -3.1592 L23:  -3.3373                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5007 S12:  -2.6346 S13:   1.3173                       
REMARK   3      S21:   1.7834 S22:  -1.6933 S23:   0.9970                       
REMARK   3      S31:   0.8995 S32:  -1.1693 S33:   0.7881                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 58 THROUGH 92 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -67.8627 132.5910 120.2220              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0693 T22:   1.6601                                     
REMARK   3      T33:   3.1647 T12:  -0.0217                                     
REMARK   3      T13:  -0.0228 T23:  -0.8804                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9343 L22:   7.6190                                     
REMARK   3      L33:   6.5593 L12:   6.5216                                     
REMARK   3      L13:  -3.8243 L23:  -2.8659                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.0408 S12:  -2.0725 S13:   0.0470                       
REMARK   3      S21:   0.3551 S22:   0.1807 S23:  -1.3950                       
REMARK   3      S31:  -0.3984 S32:   0.8218 S33:   1.1812                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 93 THROUGH 106 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -62.6398 148.1568 100.8539              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.2580 T22:   0.4760                                     
REMARK   3      T33:   5.9471 T12:   0.0467                                     
REMARK   3      T13:  -0.3543 T23:   0.8123                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.5617 L22:   6.1423                                     
REMARK   3      L33:   2.2088 L12:   3.2785                                     
REMARK   3      L13:   0.4013 L23:   2.5566                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.6009 S12:   0.3902 S13:   1.6162                       
REMARK   3      S21:  -3.3402 S22:   0.7274 S23:   1.7520                       
REMARK   3      S31:  -1.1725 S32:   0.0813 S33:  -0.2068                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 107 THROUGH 133 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -71.3660 146.4927  90.9939              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8756 T22:   1.4840                                     
REMARK   3      T33:   3.4953 T12:  -0.3602                                     
REMARK   3      T13:  -0.3668 T23:   1.0532                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3295 L22:   4.2462                                     
REMARK   3      L33:   0.9793 L12:  -0.7368                                     
REMARK   3      L13:  -0.4511 L23:   1.9448                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.9945 S12:   1.6440 S13:   2.4761                       
REMARK   3      S21:  -1.4021 S22:  -0.9976 S23:   2.3714                       
REMARK   3      S31:  -0.9385 S32:   0.2479 S33:  -0.1623                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 134 THROUGH 155 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -69.3275 139.7675  96.0840              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.9997 T22:   1.7653                                     
REMARK   3      T33:   3.0136 T12:  -0.6836                                     
REMARK   3      T13:  -1.2060 T23:   0.5669                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.4933 L22:   6.1460                                     
REMARK   3      L33:   6.5044 L12:   0.3843                                     
REMARK   3      L13:  -0.3065 L23:   3.7103                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1476 S12:  -0.2924 S13:   1.5016                       
REMARK   3      S21:  -2.2087 S22:  -0.3748 S23:   1.1929                       
REMARK   3      S31:  -2.0136 S32:   0.8819 S33:   0.3639                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 156 THROUGH 174 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -77.4008 148.4256  96.4088              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.6379 T22:   2.0516                                     
REMARK   3      T33:   3.4386 T12:   0.1607                                     
REMARK   3      T13:  -0.5481 T23:   0.0134                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.6079 L22:   9.4532                                     
REMARK   3      L33:   5.5434 L12:  -0.1807                                     
REMARK   3      L13:   1.0791 L23:   2.3272                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4183 S12:  -1.5733 S13:   3.0696                       
REMARK   3      S21:   0.9593 S22:   0.5451 S23:  -1.4867                       
REMARK   3      S31:   0.4350 S32:  -1.5257 S33:  -0.1571                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 175 THROUGH 195 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -65.0513 145.7224 101.8526              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.9374 T22:   1.5462                                     
REMARK   3      T33:   3.9422 T12:  -0.2361                                     
REMARK   3      T13:  -0.3340 T23:   0.4370                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.6357 L22:   4.7504                                     
REMARK   3      L33:   5.6775 L12:  -5.7013                                     
REMARK   3      L13:   1.5811 L23:  -1.2203                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.4028 S12:   0.1366 S13:   0.2572                       
REMARK   3      S21:   0.2615 S22:   1.0303 S23:   2.0195                       
REMARK   3      S31:  -1.3549 S32:   0.5838 S33:  -0.0320                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 1 THROUGH 56 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -27.3322 141.6816  92.9197              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.0651 T22:   1.7828                                     
REMARK   3      T33:   2.5657 T12:  -0.7356                                     
REMARK   3      T13:   0.3551 T23:   0.4522                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.9135 L22:   8.9954                                     
REMARK   3      L33:   2.7994 L12:  -6.8006                                     
REMARK   3      L13:   3.4843 L23:  -1.5004                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.1661 S12:   0.3222 S13:   3.0736                       
REMARK   3      S21:  -0.7930 S22:   1.0855 S23:  -1.0122                       
REMARK   3      S31:  -0.6135 S32:   0.0036 S33:   0.1788                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 57 THROUGH 85 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -27.9779 148.1472  82.3581              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.6125 T22:   2.2419                                     
REMARK   3      T33:   4.1007 T12:  -1.2652                                     
REMARK   3      T13:   0.0827 T23:   1.1995                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0777 L22:   0.8561                                     
REMARK   3      L33:   2.9163 L12:   0.2493                                     
REMARK   3      L13:   0.4469 L23:   1.6186                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6167 S12:   0.4083 S13:   0.3635                       
REMARK   3      S21:  -0.3390 S22:   0.0923 S23:   0.0009                       
REMARK   3      S31:  -1.9953 S32:   0.0676 S33:  -0.9945                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 86 THROUGH 126 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -37.9086 137.7882  82.3442              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.6279 T22:   2.1330                                     
REMARK   3      T33:   2.7252 T12:  -0.7818                                     
REMARK   3      T13:   0.1396 T23:   0.8229                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.3414 L22:   3.0014                                     
REMARK   3      L33:   2.6748 L12:  -1.8080                                     
REMARK   3      L13:   0.9429 L23:   0.3132                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2894 S12:   2.0451 S13:   2.0690                       
REMARK   3      S21:  -1.3070 S22:  -0.2348 S23:  -1.2736                       
REMARK   3      S31:  -0.5335 S32:  -0.3064 S33:  -0.1493                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 127 THROUGH 151 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -39.8656 138.2039  69.6337              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   3.7876 T22:   4.2472                                     
REMARK   3      T33:   3.0666 T12:  -1.8754                                     
REMARK   3      T13:  -0.6363 T23:   1.2338                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.8151 L22:   2.0340                                     
REMARK   3      L33:   4.5176 L12:   1.5459                                     
REMARK   3      L13:   5.6216 L23:   2.3186                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1689 S12:   1.3526 S13:  -0.4393                       
REMARK   3      S21:  -2.3046 S22:   2.6489 S23:   1.9828                       
REMARK   3      S31:   0.5279 S32:   2.0080 S33:  -2.4751                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 152 THROUGH 197 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -31.1896 125.7116  94.2896              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8189 T22:   1.2328                                     
REMARK   3      T33:   1.8345 T12:   0.1103                                     
REMARK   3      T13:   0.4792 T23:   0.0636                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.0355 L22:   4.9354                                     
REMARK   3      L33:   5.3550 L12:   2.0399                                     
REMARK   3      L13:  -2.5735 L23:  -0.5483                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.7475 S12:   1.7210 S13:   1.3541                       
REMARK   3      S21:  -2.0226 S22:   0.1056 S23:  -1.2841                       
REMARK   3      S31:  -1.3580 S32:   0.3828 S33:  -0.6689                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 198 THROUGH 227 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -48.8618 119.0403 105.9748              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1719 T22:   1.0861                                     
REMARK   3      T33:   1.5124 T12:  -0.2463                                     
REMARK   3      T13:  -0.1742 T23:   0.2078                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.8405 L22:   5.1721                                     
REMARK   3      L33:   4.6879 L12:   1.3810                                     
REMARK   3      L13:  -4.1452 L23:   1.6804                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5779 S12:   1.7888 S13:   0.9757                       
REMARK   3      S21:  -1.1736 S22:  -0.1734 S23:   1.5664                       
REMARK   3      S31:  -0.6653 S32:  -1.5377 S33:   0.4950                       
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 228 THROUGH 262 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -47.8564 121.4954 108.3889              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0348 T22:   0.7735                                     
REMARK   3      T33:   2.0579 T12:  -0.1546                                     
REMARK   3      T13:  -0.1025 T23:  -0.1820                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.2817 L22:   2.0217                                     
REMARK   3      L33:   7.5217 L12:  -2.1511                                     
REMARK   3      L13:  -0.5440 L23:   0.5331                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5409 S12:   0.8174 S13:   1.4866                       
REMARK   3      S21:  -1.1046 S22:   0.6691 S23:   0.3767                       
REMARK   3      S31:  -0.2238 S32:  -0.6604 S33:  -0.1308                       
REMARK   3   TLS GROUP : 16                                                     
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 263 THROUGH 276 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -41.8953 112.5073 109.2807              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8727 T22:   0.7525                                     
REMARK   3      T33:   1.2899 T12:  -0.0600                                     
REMARK   3      T13:  -0.0126 T23:   0.3422                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.3158 L22:   7.2543                                     
REMARK   3      L33:   5.2106 L12:  -3.8306                                     
REMARK   3      L13:  -3.6617 L23:   5.4062                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6112 S12:  -1.3226 S13:  -1.2641                       
REMARK   3      S21:   0.4290 S22:   0.1375 S23:   2.2449                       
REMARK   3      S31:   1.1499 S32:   0.6694 S33:   0.1374                       
REMARK   3   TLS GROUP : 17                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID -1 THROUGH 8 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -49.1473 142.8992  96.3834              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.6365 T22:   1.3337                                     
REMARK   3      T33:   4.1841 T12:  -1.1591                                     
REMARK   3      T13:   0.0326 T23:  -0.6480                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.9263 L22:   5.9189                                     
REMARK   3      L33:   3.5610 L12:  -2.2332                                     
REMARK   3      L13:   0.8596 L23:   3.9248                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5526 S12:   1.4469 S13:   1.7919                       
REMARK   3      S21:  -2.5754 S22:  -0.0971 S23:   0.7689                       
REMARK   3      S31:  -1.8085 S32:  -0.5670 S33:  -0.5121                       
REMARK   3   TLS GROUP : 18                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 9 THROUGH 27 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -35.9771 134.0754 115.2108              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.2340 T22:   1.3826                                     
REMARK   3      T33:   1.8321 T12:  -0.2633                                     
REMARK   3      T13:  -0.2839 T23:  -0.3935                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.9559 L22:   8.4021                                     
REMARK   3      L33:   6.8685 L12:  -0.3124                                     
REMARK   3      L13:  -7.4658 L23:  -1.8349                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3448 S12:  -1.7194 S13:   3.5569                       
REMARK   3      S21:   0.3857 S22:   0.4141 S23:   1.4761                       
REMARK   3      S31:   0.1627 S32:   2.2481 S33:  -0.6957                       
REMARK   3   TLS GROUP : 19                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 28 THROUGH 38 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -43.2309 146.9012  99.9062              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3959 T22:   1.1726                                     
REMARK   3      T33:   3.3533 T12:  -0.1792                                     
REMARK   3      T13:  -0.0691 T23:   0.2011                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.2703 L22:   3.1014                                     
REMARK   3      L33:   3.6284 L12:  -0.8216                                     
REMARK   3      L13:  -0.1355 L23:  -2.7938                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2850 S12:  -0.1595 S13:   4.9498                       
REMARK   3      S21:  -0.6557 S22:   1.2660 S23:  -0.3678                       
REMARK   3      S31:  -2.2341 S32:  -0.6612 S33:  -1.5360                       
REMARK   3   TLS GROUP : 20                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 39 THROUGH 46 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -39.2831 148.3585 118.5458              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7736 T22:   1.7721                                     
REMARK   3      T33:   2.9992 T12:  -0.4406                                     
REMARK   3      T13:   0.0017 T23:  -1.0326                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.0404 L22:   4.8736                                     
REMARK   3      L33:   8.3390 L12:   0.2367                                     
REMARK   3      L13:  -0.0231 L23:  -6.3116                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6838 S12:  -0.7488 S13:   2.5528                       
REMARK   3      S21:   1.8891 S22:  -0.5084 S23:  -1.3837                       
REMARK   3      S31:  -3.9290 S32:   2.3479 S33:  -0.1598                       
REMARK   3   TLS GROUP : 21                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 47 THROUGH 56 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -32.7658 143.9876 103.6779              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7128 T22:   1.1274                                     
REMARK   3      T33:   3.3656 T12:  -0.6121                                     
REMARK   3      T13:  -0.1105 T23:   0.0513                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.4839 L22:   3.8281                                     
REMARK   3      L33:   9.2840 L12:  -2.2684                                     
REMARK   3      L13:   4.6398 L23:   1.8742                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.1286 S12:  -1.2109 S13:   2.7586                       
REMARK   3      S21:  -0.4084 S22:   0.6592 S23:  -1.8328                       
REMARK   3      S31:   0.3294 S32:  -0.3344 S33:   0.8483                       
REMARK   3   TLS GROUP : 22                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 57 THROUGH 63 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -41.9187 137.8186  91.9141              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.2593 T22:   1.1033                                     
REMARK   3      T33:   2.4747 T12:  -2.0584                                     
REMARK   3      T13:  -0.2314 T23:   2.5703                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2473 L22:   1.0475                                     
REMARK   3      L33:   0.5827 L12:   0.0256                                     
REMARK   3      L13:  -0.1333 L23:   0.1983                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0731 S12:   0.6056 S13:  -0.3639                       
REMARK   3      S21:  -0.9357 S22:  -0.1424 S23:   0.3583                       
REMARK   3      S31:   0.0238 S32:  -0.4586 S33:  -0.2537                       
REMARK   3   TLS GROUP : 23                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 64 THROUGH 77 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -36.4851 140.0991 118.1041              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8134 T22:   1.0493                                     
REMARK   3      T33:   2.5177 T12:  -1.1415                                     
REMARK   3      T13:  -0.1912 T23:  -0.6076                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.1450 L22:   3.6374                                     
REMARK   3      L33:   0.9344 L12:  -1.3875                                     
REMARK   3      L13:   2.8960 L23:  -0.2901                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2574 S12:  -4.4012 S13:   0.4837                       
REMARK   3      S21:   1.7482 S22:   0.5123 S23:   0.7627                       
REMARK   3      S31:  -1.0338 S32:  -0.2270 S33:   0.0679                       
REMARK   3   TLS GROUP : 24                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 78 THROUGH 90 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -47.7932 148.4359 107.7661              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.4407 T22:   1.0429                                     
REMARK   3      T33:   3.9444 T12:  -0.1304                                     
REMARK   3      T13:  -0.1067 T23:   0.2046                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.6281 L22:   9.1300                                     
REMARK   3      L33:   3.9848 L12:  -4.7104                                     
REMARK   3      L13:   3.1854 L23:  -5.7459                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.3392 S12:  -0.3059 S13:   3.9360                       
REMARK   3      S21:  -0.4795 S22:   1.6903 S23:   2.9338                       
REMARK   3      S31:  -1.6932 S32:  -1.2625 S33:   0.2486                       
REMARK   3   TLS GROUP : 25                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 91 THROUGH 99 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -45.7889 136.5957 116.8338              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.2541 T22:   1.5868                                     
REMARK   3      T33:   2.4484 T12:  -0.2029                                     
REMARK   3      T13:  -0.0356 T23:  -0.5181                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.3378 L22:   8.1267                                     
REMARK   3      L33:   3.6570 L12:   2.0200                                     
REMARK   3      L13:  -2.1766 L23:  -2.6111                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   1.2538 S12:  -0.1772 S13:   1.6710                       
REMARK   3      S21:   0.3040 S22:   0.4124 S23:   0.8912                       
REMARK   3      S31:  -0.2347 S32:   2.4949 S33:  -1.6726                       
REMARK   3   TLS GROUP : 26                                                     
REMARK   3    SELECTION: CHAIN 'N' AND (RESID 2 THROUGH 7 )                     
REMARK   3    ORIGIN FOR THE GROUP (A): -28.1726 142.2397  77.3012              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   3.8594 T22:   2.1749                                     
REMARK   3      T33:   5.6672 T12:  -1.7155                                     
REMARK   3      T13:  -0.1917 T23:   1.4669                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.4026 L22:   4.9219                                     
REMARK   3      L33:   2.3764 L12:   2.8010                                     
REMARK   3      L13:   2.6630 L23:   3.3316                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1886 S12:   0.5576 S13:  -0.4418                       
REMARK   3      S21:   1.1930 S22:   0.4751 S23:   2.3248                       
REMARK   3      S31:   2.1991 S32:  -1.8964 S33:   0.4785                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5KNM COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-JUL-16.                  
REMARK 100 THE DEPOSITION ID IS D_1000222483.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 20-NOV-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 23-ID-D                            
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.03318                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 6M                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XIA2                               
REMARK 200  DATA SCALING SOFTWARE          : XIA2                               
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 17859                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.304                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 49.406                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.0                               
REMARK 200  DATA REDUNDANCY                : 9.400                              
REMARK 200  R MERGE                    (I) : 0.14130                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 1.5700                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.30                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.42                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.0                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 9.80                               
REMARK 200  R MERGE FOR SHELL          (I) : 1.13600                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.570                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1PSA                                                 
REMARK 200                                                                      
REMARK 200 REMARK: HEXAGONAL PLATES                                             
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 67.59                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.42                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 27% PEG 550 MME .1 M TRIS-HCL 300 MM     
REMARK 280  NACL, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K       
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 6 2 2                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z                                                
REMARK 290       3555   -X+Y,-X,Z                                               
REMARK 290       4555   -X,-Y,Z                                                 
REMARK 290       5555   Y,-X+Y,Z                                                
REMARK 290       6555   X-Y,X,Z                                                 
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   X-Y,-Y,-Z                                               
REMARK 290       9555   -X,-X+Y,-Z                                              
REMARK 290      10555   -Y,-X,-Z                                                
REMARK 290      11555   -X+Y,Y,-Z                                               
REMARK 290      12555   X,X-Y,-Z                                                
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, D, N                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A   277                                                      
REMARK 465     PRO A   278                                                      
REMARK 465     GLN A   279                                                      
REMARK 465     PRO A   280                                                      
REMARK 465     THR A   281                                                      
REMARK 465     ILE A   282                                                      
REMARK 465     PRO A   283                                                      
REMARK 465     ILE A   284                                                      
REMARK 465     MET B   -68                                                      
REMARK 465     LEU B   -67                                                      
REMARK 465     LEU B   -66                                                      
REMARK 465     VAL B   -65                                                      
REMARK 465     ASN B   -64                                                      
REMARK 465     GLN B   -63                                                      
REMARK 465     SER B   -62                                                      
REMARK 465     HIS B   -61                                                      
REMARK 465     GLN B   -60                                                      
REMARK 465     GLY B   -59                                                      
REMARK 465     PHE B   -58                                                      
REMARK 465     ASN B   -57                                                      
REMARK 465     LYS B   -56                                                      
REMARK 465     GLU B   -55                                                      
REMARK 465     HIS B   -54                                                      
REMARK 465     THR B   -53                                                      
REMARK 465     SER B   -52                                                      
REMARK 465     LYS B   -51                                                      
REMARK 465     MET B   -50                                                      
REMARK 465     VAL B   -49                                                      
REMARK 465     SER B   -48                                                      
REMARK 465     ALA B   -47                                                      
REMARK 465     ILE B   -46                                                      
REMARK 465     VAL B   -45                                                      
REMARK 465     LEU B   -44                                                      
REMARK 465     TYR B   -43                                                      
REMARK 465     VAL B   -42                                                      
REMARK 465     LEU B   -41                                                      
REMARK 465     LEU B   -40                                                      
REMARK 465     ALA B   -39                                                      
REMARK 465     ALA B   -38                                                      
REMARK 465     ALA B   -37                                                      
REMARK 465     ALA B   -36                                                      
REMARK 465     HIS B   -35                                                      
REMARK 465     SER B   -34                                                      
REMARK 465     ALA B   -33                                                      
REMARK 465     PHE B   -32                                                      
REMARK 465     ALA B   -31                                                      
REMARK 465     ALA B   -30                                                      
REMARK 465     ASP B   -29                                                      
REMARK 465     LEU B   -28                                                      
REMARK 465     HIS B   -27                                                      
REMARK 465     HIS B   -26                                                      
REMARK 465     HIS B   -25                                                      
REMARK 465     HIS B   -24                                                      
REMARK 465     HIS B   -23                                                      
REMARK 465     HIS B   -22                                                      
REMARK 465     HIS B   -21                                                      
REMARK 465     HIS B   -20                                                      
REMARK 465     GLY B   -19                                                      
REMARK 465     SER B   -18                                                      
REMARK 465     GLY B   -17                                                      
REMARK 465     GLY B   -16                                                      
REMARK 465     LEU B   -15                                                      
REMARK 465     GLU B   -14                                                      
REMARK 465     VAL B   -13                                                      
REMARK 465     LEU B   -12                                                      
REMARK 465     PHE B   -11                                                      
REMARK 465     GLN B   -10                                                      
REMARK 465     GLY B    -9                                                      
REMARK 465     PRO B    -8                                                      
REMARK 465     GLU B    -7                                                      
REMARK 465     PHE B    -6                                                      
REMARK 465     GLY B    -5                                                      
REMARK 465     GLY B    -4                                                      
REMARK 465     SER B    -3                                                      
REMARK 465     ALA B    -2                                                      
REMARK 465     GLY B   100                                                      
REMARK 465     GLY B   101                                                      
REMARK 465     GLY B   102                                                      
REMARK 465     GLY B   103                                                      
REMARK 465     SER B   104                                                      
REMARK 465     GLY B   105                                                      
REMARK 465     GLY B   106                                                      
REMARK 465     SER B   107                                                      
REMARK 465     GLY B   108                                                      
REMARK 465     SER B   109                                                      
REMARK 465     GLY B   110                                                      
REMARK 465     GLY B   111                                                      
REMARK 465     GLY B   112                                                      
REMARK 465     SER B   113                                                      
REMARK 465     MET D   -68                                                      
REMARK 465     LEU D   -67                                                      
REMARK 465     LEU D   -66                                                      
REMARK 465     VAL D   -65                                                      
REMARK 465     ASN D   -64                                                      
REMARK 465     GLN D   -63                                                      
REMARK 465     SER D   -62                                                      
REMARK 465     HIS D   -61                                                      
REMARK 465     GLN D   -60                                                      
REMARK 465     GLY D   -59                                                      
REMARK 465     PHE D   -58                                                      
REMARK 465     ASN D   -57                                                      
REMARK 465     LYS D   -56                                                      
REMARK 465     GLU D   -55                                                      
REMARK 465     HIS D   -54                                                      
REMARK 465     THR D   -53                                                      
REMARK 465     SER D   -52                                                      
REMARK 465     LYS D   -51                                                      
REMARK 465     MET D   -50                                                      
REMARK 465     VAL D   -49                                                      
REMARK 465     SER D   -48                                                      
REMARK 465     ALA D   -47                                                      
REMARK 465     ILE D   -46                                                      
REMARK 465     VAL D   -45                                                      
REMARK 465     LEU D   -44                                                      
REMARK 465     TYR D   -43                                                      
REMARK 465     VAL D   -42                                                      
REMARK 465     LEU D   -41                                                      
REMARK 465     LEU D   -40                                                      
REMARK 465     ALA D   -39                                                      
REMARK 465     ALA D   -38                                                      
REMARK 465     ALA D   -37                                                      
REMARK 465     ALA D   -36                                                      
REMARK 465     HIS D   -35                                                      
REMARK 465     SER D   -34                                                      
REMARK 465     ALA D   -33                                                      
REMARK 465     PHE D   -32                                                      
REMARK 465     ALA D   -31                                                      
REMARK 465     ALA D   -30                                                      
REMARK 465     ASP D   -29                                                      
REMARK 465     LEU D   -28                                                      
REMARK 465     HIS D   -27                                                      
REMARK 465     HIS D   -26                                                      
REMARK 465     HIS D   -25                                                      
REMARK 465     HIS D   -24                                                      
REMARK 465     HIS D   -23                                                      
REMARK 465     HIS D   -22                                                      
REMARK 465     HIS D   -21                                                      
REMARK 465     HIS D   -20                                                      
REMARK 465     GLY D   -19                                                      
REMARK 465     SER D   -18                                                      
REMARK 465     GLY D   -17                                                      
REMARK 465     GLY D   -16                                                      
REMARK 465     LEU D   -15                                                      
REMARK 465     GLU D   -14                                                      
REMARK 465     VAL D   -13                                                      
REMARK 465     LEU D   -12                                                      
REMARK 465     PHE D   -11                                                      
REMARK 465     GLN D   -10                                                      
REMARK 465     GLY D    -9                                                      
REMARK 465     PRO D    -8                                                      
REMARK 465     GLU D    -7                                                      
REMARK 465     PHE D    -6                                                      
REMARK 465     GLY D    -5                                                      
REMARK 465     GLY D    -4                                                      
REMARK 465     SER D    -3                                                      
REMARK 465     ALA D    -2                                                      
REMARK 465     ASP D    -1                                                      
REMARK 465     LEU D     0                                                      
REMARK 465     GLU D   138                                                      
REMARK 465     ASP D   139                                                      
REMARK 465     GLU D   140                                                      
REMARK 465     SER D   160                                                      
REMARK 465     VAL D   161                                                      
REMARK 465     GLY D   162                                                      
REMARK 465     PRO D   163                                                      
REMARK 465     VAL D   164                                                      
REMARK 465     VAL D   196                                                      
REMARK 465     LEU D   197                                                      
REMARK 465     GLY D   198                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A  44    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     TYR A  85    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LYS A 121    CG   CD   CE   NZ                                   
REMARK 470     TYR A 123    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     TRP A 133    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP A 133    CZ3  CH2                                            
REMARK 470     ASP A 137    CG   OD1  OD2                                       
REMARK 470     ARG A 169    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLN B   2    CG   CD   OE1  NE2                                  
REMARK 470     HIS D   2    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLN D  27    CG   CD   OE1  NE2                                  
REMARK 470     THR D  32    OG1  CG2                                            
REMARK 470     LYS D  42    CG   CD   CE   NZ                                   
REMARK 470     ARG D  49    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS D  57    CG   CD   CE   NZ                                   
REMARK 470     GLN D  59    CG   CD   OE1  NE2                                  
REMARK 470     ARG D  74    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS D 135    CG   CD   CE   NZ                                   
REMARK 470     GLU D 136    CG   CD   OE1  OE2                                  
REMARK 470     HIS D 141    CG   ND1  CD2  CE1  NE2                             
REMARK 470     TRP D 170    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP D 170    CZ3  CH2                                            
REMARK 470     TYR D 172    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     GLU D 193    CG   CD   OE1  OE2                                  
REMARK 470     ILE N   2    CG1  CG2  CD1                                       
REMARK 470     LEU N   3    CG   CD1  CD2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    GLN D 143   CA  -  CB  -  CG  ANGL. DEV. =  13.9 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASP A  29     -121.76     49.95                                   
REMARK 500    ILE A  42       67.61     36.64                                   
REMARK 500    ASN A  86       67.07     35.64                                   
REMARK 500    LEU A 110      -75.03   -101.54                                   
REMARK 500    TYR A 123      -64.91   -103.28                                   
REMARK 500    ARG A 131      -72.29   -121.83                                   
REMARK 500    ALA A 136      -70.93   -130.87                                   
REMARK 500    GLU A 161      -73.00    -88.96                                   
REMARK 500    THR A 178      -59.03   -126.42                                   
REMARK 500    PRO A 210     -174.25    -67.52                                   
REMARK 500    ILE B   1     -152.41     47.56                                   
REMARK 500    GLN B   2      164.62    170.14                                   
REMARK 500    HIS B  31      137.74   -175.35                                   
REMARK 500    SER B  88      -73.99    -89.17                                   
REMARK 500    ASP B  98      -63.48   -125.47                                   
REMARK 500    GLN D  30     -146.85     50.86                                   
REMARK 500    LYS D  41     -116.29     57.57                                   
REMARK 500    ILE D  62       75.26     51.70                                   
REMARK 500    SER D  64     -140.40     52.90                                   
REMARK 500    ILE D  65      160.71    179.56                                   
REMARK 500    SER D  79     -131.32     56.56                                   
REMARK 500    THR D  81       -6.81     62.64                                   
REMARK 500    ALA D  82      -65.09   -126.73                                   
REMARK 500    ALA D  98       -2.76     69.83                                   
REMARK 500    GLN D 107     -167.57    -74.58                                   
REMARK 500    PRO D 108     -176.62    -54.34                                   
REMARK 500    SER D 114       -4.64     70.01                                   
REMARK 500    ASN D 117     -139.54     61.26                                   
REMARK 500    ASP D 129      -71.78    -87.60                                   
REMARK 500    LYS D 135      -70.53    -82.53                                   
REMARK 500    GLN D 143      -66.12   -120.89                                   
REMARK 500    HIS D 150      -70.92    -76.69                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 610                                                                      
REMARK 610 MISSING HETEROATOM                                                   
REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 610 I=INSERTION CODE):                                                   
REMARK 610   M RES C SSEQI                                                      
REMARK 610     NAG A  301                                                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue NAG A 301                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5IUE   RELATED DB: PDB                                   
DBREF  5KNM A    1   284  UNP    B3KUD8   B3KUD8_HUMAN    22    305             
DBREF  5KNM B    1    99  UNP    P61769   B2MG_HUMAN      21    119             
DBREF  5KNM D    1   198  UNP    Q8NHL6   LIRB1_HUMAN     24    221             
DBREF  5KNM N    2     7  PDB    5KNM     5KNM             2      7             
SEQADV 5KNM MET B  -68  UNP  P61769              INITIATING METHIONINE          
SEQADV 5KNM LEU B  -67  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -66  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM VAL B  -65  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ASN B  -64  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLN B  -63  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  -62  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -61  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLN B  -60  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  -59  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM PHE B  -58  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ASN B  -57  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LYS B  -56  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLU B  -55  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -54  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM THR B  -53  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  -52  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LYS B  -51  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM MET B  -50  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM VAL B  -49  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  -48  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -47  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ILE B  -46  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM VAL B  -45  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -44  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM TYR B  -43  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM VAL B  -42  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -41  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -40  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -39  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -38  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -37  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -36  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -35  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  -34  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -33  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM PHE B  -32  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -31  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B  -30  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ASP B  -29  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -28  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -27  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -26  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -25  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -24  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -23  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -22  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -21  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM HIS B  -20  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  -19  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  -18  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  -17  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  -16  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -15  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLU B  -14  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM VAL B  -13  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM LEU B  -12  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM PHE B  -11  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLN B  -10  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B   -9  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM PRO B   -8  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLU B   -7  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM PHE B   -6  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B   -5  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B   -4  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B   -3  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ALA B   -2  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM ASP B   -1  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM PRO B    0  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  100  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  101  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  102  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  103  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  104  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  105  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  106  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  107  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  108  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  109  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  110  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  111  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM GLY B  112  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM SER B  113  UNP  P61769              EXPRESSION TAG                 
SEQADV 5KNM MET D  -68  UNP  Q8NHL6              INITIATING METHIONINE          
SEQADV 5KNM LEU D  -67  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -66  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM VAL D  -65  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ASN D  -64  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLN D  -63  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM SER D  -62  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -61  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLN D  -60  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D  -59  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM PHE D  -58  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ASN D  -57  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LYS D  -56  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLU D  -55  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -54  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM THR D  -53  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM SER D  -52  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LYS D  -51  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM MET D  -50  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM VAL D  -49  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM SER D  -48  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -47  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ILE D  -46  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM VAL D  -45  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -44  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM TYR D  -43  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM VAL D  -42  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -41  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -40  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -39  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -38  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -37  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -36  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -35  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM SER D  -34  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -33  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM PHE D  -32  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -31  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D  -30  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ASP D  -29  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -28  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -27  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -26  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -25  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -24  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -23  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -22  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -21  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM HIS D  -20  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D  -19  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM SER D  -18  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D  -17  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D  -16  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -15  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLU D  -14  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM VAL D  -13  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D  -12  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM PHE D  -11  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLN D  -10  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D   -9  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM PRO D   -8  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLU D   -7  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM PHE D   -6  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D   -5  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM GLY D   -4  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM SER D   -3  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ALA D   -2  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM ASP D   -1  UNP  Q8NHL6              EXPRESSION TAG                 
SEQADV 5KNM LEU D    0  UNP  Q8NHL6              EXPRESSION TAG                 
SEQRES   1 A  284  GLY SER HIS SER LEU ARG TYR PHE SER THR ALA VAL SER          
SEQRES   2 A  284  ARG PRO GLY ARG GLY GLU PRO ARG TYR ILE ALA VAL GLU          
SEQRES   3 A  284  TYR VAL ASP ASP THR GLN PHE LEU ARG PHE ASP SER ASP          
SEQRES   4 A  284  ALA ALA ILE PRO ARG MET GLU PRO ARG GLU PRO TRP VAL          
SEQRES   5 A  284  GLU GLN GLU GLY PRO GLN TYR TRP GLU TRP THR THR GLY          
SEQRES   6 A  284  TYR ALA LYS ALA ASN ALA GLN THR ASP ARG VAL ALA LEU          
SEQRES   7 A  284  ARG ASN LEU LEU ARG ARG TYR ASN GLN SER GLU ALA GLY          
SEQRES   8 A  284  SER HIS THR LEU GLN GLY MET ASN GLY CYS ASP MET GLY          
SEQRES   9 A  284  PRO ASP GLY ARG LEU LEU ARG GLY TYR HIS GLN HIS ALA          
SEQRES  10 A  284  TYR ASP GLY LYS ASP TYR ILE SER LEU ASN GLU ASP LEU          
SEQRES  11 A  284  ARG SER TRP THR ALA ALA ASP THR VAL ALA GLN ILE THR          
SEQRES  12 A  284  GLN ARG PHE TYR GLU ALA GLU GLU TYR ALA GLU GLU PHE          
SEQRES  13 A  284  ARG THR TYR LEU GLU GLY GLU CYS LEU GLU LEU LEU ARG          
SEQRES  14 A  284  ARG TYR LEU GLU ASN GLY LYS GLU THR LEU GLN ARG ALA          
SEQRES  15 A  284  ASP PRO PRO LYS ALA HIS VAL ALA HIS HIS PRO ILE SER          
SEQRES  16 A  284  ASP HIS GLU ALA THR LEU ARG CYS TRP ALA LEU GLY PHE          
SEQRES  17 A  284  TYR PRO ALA GLU ILE THR LEU THR TRP GLN ARG ASP GLY          
SEQRES  18 A  284  GLU GLU GLN THR GLN ASP THR GLU LEU VAL GLU THR ARG          
SEQRES  19 A  284  PRO ALA GLY ASP GLY THR PHE GLN LYS TRP ALA ALA VAL          
SEQRES  20 A  284  VAL VAL PRO SER GLY GLU GLU GLN ARG TYR THR CYS HIS          
SEQRES  21 A  284  VAL GLN HIS GLU GLY LEU PRO GLN PRO LEU ILE LEU ARG          
SEQRES  22 A  284  TRP GLU GLN SER PRO GLN PRO THR ILE PRO ILE                  
SEQRES   1 B  182  MET LEU LEU VAL ASN GLN SER HIS GLN GLY PHE ASN LYS          
SEQRES   2 B  182  GLU HIS THR SER LYS MET VAL SER ALA ILE VAL LEU TYR          
SEQRES   3 B  182  VAL LEU LEU ALA ALA ALA ALA HIS SER ALA PHE ALA ALA          
SEQRES   4 B  182  ASP LEU HIS HIS HIS HIS HIS HIS HIS HIS GLY SER GLY          
SEQRES   5 B  182  GLY LEU GLU VAL LEU PHE GLN GLY PRO GLU PHE GLY GLY          
SEQRES   6 B  182  SER ALA ASP PRO ILE GLN ARG THR PRO LYS ILE GLN VAL          
SEQRES   7 B  182  TYR SER ARG HIS PRO ALA GLU ASN GLY LYS SER ASN PHE          
SEQRES   8 B  182  LEU ASN CYS TYR VAL SER GLY PHE HIS PRO SER ASP ILE          
SEQRES   9 B  182  GLU VAL ASP LEU LEU LYS ASN GLY GLU ARG ILE GLU LYS          
SEQRES  10 B  182  VAL GLU HIS SER ASP LEU SER PHE SER LYS ASP TRP SER          
SEQRES  11 B  182  PHE TYR LEU LEU TYR TYR THR GLU PHE THR PRO THR GLU          
SEQRES  12 B  182  LYS ASP GLU TYR ALA CYS ARG VAL ASN HIS VAL THR LEU          
SEQRES  13 B  182  SER GLN PRO LYS ILE VAL LYS TRP ASP ARG ASP MET GLY          
SEQRES  14 B  182  GLY GLY GLY SER GLY GLY SER GLY SER GLY GLY GLY SER          
SEQRES   1 D  267  MET LEU LEU VAL ASN GLN SER HIS GLN GLY PHE ASN LYS          
SEQRES   2 D  267  GLU HIS THR SER LYS MET VAL SER ALA ILE VAL LEU TYR          
SEQRES   3 D  267  VAL LEU LEU ALA ALA ALA ALA HIS SER ALA PHE ALA ALA          
SEQRES   4 D  267  ASP LEU HIS HIS HIS HIS HIS HIS HIS HIS GLY SER GLY          
SEQRES   5 D  267  GLY LEU GLU VAL LEU PHE GLN GLY PRO GLU PHE GLY GLY          
SEQRES   6 D  267  SER ALA ASP LEU GLY HIS LEU PRO LYS PRO THR LEU TRP          
SEQRES   7 D  267  ALA GLU PRO GLY SER VAL ILE THR GLN GLY SER PRO VAL          
SEQRES   8 D  267  THR LEU ARG CYS GLN GLY GLY GLN GLU THR GLN GLU TYR          
SEQRES   9 D  267  ARG LEU TYR ARG GLU LYS LYS THR ALA LEU TRP ILE THR          
SEQRES  10 D  267  ARG ILE PRO GLN GLU LEU VAL LYS LYS GLY GLN PHE PRO          
SEQRES  11 D  267  ILE PRO SER ILE THR TRP GLU HIS ALA GLY ARG TYR ARG          
SEQRES  12 D  267  CYS TYR TYR GLY SER ASP THR ALA GLY ARG SER GLU SER          
SEQRES  13 D  267  SER ASP PRO LEU GLU LEU VAL VAL THR GLY ALA TYR ILE          
SEQRES  14 D  267  LYS PRO THR LEU SER ALA GLN PRO SER PRO VAL VAL ASN          
SEQRES  15 D  267  SER GLY GLY ASN VAL ILE LEU GLN CYS ASP SER GLN VAL          
SEQRES  16 D  267  ALA PHE ASP GLY PHE SER LEU CYS LYS GLU GLY GLU ASP          
SEQRES  17 D  267  GLU HIS PRO GLN CYS LEU ASN SER GLN PRO HIS ALA ARG          
SEQRES  18 D  267  GLY SER SER ARG ALA ILE PHE SER VAL GLY PRO VAL SER          
SEQRES  19 D  267  PRO SER ARG ARG TRP TRP TYR ARG CYS TYR ALA TYR ASP          
SEQRES  20 D  267  SER ASN SER PRO TYR GLU TRP SER LEU PRO SER ASP LEU          
SEQRES  21 D  267  LEU GLU LEU LEU VAL LEU GLY                                  
SEQRES   1 N    6  ILE LEU ARG TRP GLU GLN                                      
HET    NAG  A 301      14                                                       
HETNAM     NAG N-ACETYL-D-GLUCOSAMINE                                           
FORMUL   5  NAG    C8 H15 N O6                                                  
HELIX    1 AA1 GLU A   49  GLU A   53  5                                   5    
HELIX    2 AA2 GLY A   56  ARG A   83  1                                  28    
HELIX    3 AA3 VAL A  139  GLU A  151  1                                  13    
HELIX    4 AA4 GLU A  151  LEU A  160  1                                  10    
HELIX    5 AA5 GLY A  162  GLY A  175  1                                  14    
HELIX    6 AA6 GLY A  175  GLN A  180  1                                   6    
HELIX    7 AA7 GLU A  253  GLN A  255  5                                   3    
HELIX    8 AA8 PRO D   51  LYS D   56  1                                   6    
HELIX    9 AA9 THR D   66  ALA D   70  5                                   5    
SHEET    1 AA1 8 MET A  45  PRO A  47  0                                        
SHEET    2 AA1 8 THR A  31  ASP A  37 -1  N  ARG A  35   O  GLU A  46           
SHEET    3 AA1 8 ARG A  21  VAL A  28 -1  N  VAL A  28   O  THR A  31           
SHEET    4 AA1 8 HIS A   3  VAL A  12 -1  N  ARG A   6   O  TYR A  27           
SHEET    5 AA1 8 THR A  94  MET A 103 -1  O  MET A 103   N  HIS A   3           
SHEET    6 AA1 8 TYR A 113  TYR A 118 -1  O  ALA A 117   N  GLN A  96           
SHEET    7 AA1 8 ILE A 124  LEU A 126 -1  O  ILE A 124   N  HIS A 116           
SHEET    8 AA1 8 TRP A 133  ALA A 135 -1  O  THR A 134   N  SER A 125           
SHEET    1 AA2 4 LYS A 186  SER A 195  0                                        
SHEET    2 AA2 4 GLU A 198  PHE A 208 -1  O  THR A 200   N  HIS A 192           
SHEET    3 AA2 4 PHE A 241  PRO A 250 -1  O  LYS A 243   N  ALA A 205           
SHEET    4 AA2 4 GLU A 229  LEU A 230 -1  N  GLU A 229   O  ALA A 246           
SHEET    1 AA3 4 LYS A 186  SER A 195  0                                        
SHEET    2 AA3 4 GLU A 198  PHE A 208 -1  O  THR A 200   N  HIS A 192           
SHEET    3 AA3 4 PHE A 241  PRO A 250 -1  O  LYS A 243   N  ALA A 205           
SHEET    4 AA3 4 ARG A 234  PRO A 235 -1  N  ARG A 234   O  GLN A 242           
SHEET    1 AA4 4 GLU A 222  GLU A 223  0                                        
SHEET    2 AA4 4 ILE A 213  ARG A 219 -1  N  ARG A 219   O  GLU A 222           
SHEET    3 AA4 4 TYR A 257  HIS A 263 -1  O  THR A 258   N  GLN A 218           
SHEET    4 AA4 4 LEU A 270  LEU A 272 -1  O  LEU A 272   N  CYS A 259           
SHEET    1 AA5 4 LYS B   6  SER B  11  0                                        
SHEET    2 AA5 4 ASN B  21  PHE B  30 -1  O  SER B  28   N  LYS B   6           
SHEET    3 AA5 4 PHE B  62  PHE B  70 -1  O  TYR B  66   N  CYS B  25           
SHEET    4 AA5 4 GLU B  50  HIS B  51 -1  N  GLU B  50   O  TYR B  67           
SHEET    1 AA6 4 LYS B   6  SER B  11  0                                        
SHEET    2 AA6 4 ASN B  21  PHE B  30 -1  O  SER B  28   N  LYS B   6           
SHEET    3 AA6 4 PHE B  62  PHE B  70 -1  O  TYR B  66   N  CYS B  25           
SHEET    4 AA6 4 SER B  55  PHE B  56 -1  N  SER B  55   O  TYR B  63           
SHEET    1 AA7 4 GLU B  44  ARG B  45  0                                        
SHEET    2 AA7 4 GLU B  36  LYS B  41 -1  N  LYS B  41   O  GLU B  44           
SHEET    3 AA7 4 TYR B  78  ASN B  83 -1  O  ASN B  83   N  GLU B  36           
SHEET    4 AA7 4 LYS B  91  LYS B  94 -1  O  LYS B  91   N  VAL B  82           
SHEET    1 AA8 2 THR D   7  GLU D  11  0                                        
SHEET    2 AA8 2 THR D  23  GLN D  27 -1  O  THR D  23   N  GLU D  11           
SHEET    1 AA9 4 VAL D  15  THR D  17  0                                        
SHEET    2 AA9 4 GLU D  92  THR D  96  1  O  VAL D  94   N  ILE D  16           
SHEET    3 AA9 4 GLY D  71  TYR D  77 -1  N  GLY D  71   O  LEU D  93           
SHEET    4 AA9 4 TYR D  35  ARG D  39 -1  N  ARG D  36   O  TYR D  76           
SHEET    1 AB1 3 VAL D  15  THR D  17  0                                        
SHEET    2 AB1 3 GLU D  92  THR D  96  1  O  VAL D  94   N  ILE D  16           
SHEET    3 AB1 3 GLU D 184  TRP D 185  1  O  TRP D 185   N  VAL D  95           
SHEET    1 AB2 3 THR D 103  LEU D 104  0                                        
SHEET    2 AB2 3 GLN D 121  ASP D 123 -1  O  ASP D 123   N  THR D 103           
SHEET    3 AB2 3 ARG D 156  ILE D 158 -1  O  ALA D 157   N  CYS D 122           
SHEET    1 AB3 3 CYS D 144  SER D 147  0                                        
SHEET    2 AB3 3 GLY D 130  CYS D 134 -1  N  LEU D 133   O  LEU D 145           
SHEET    3 AB3 3 ARG D 173  TYR D 177 -1  O  TYR D 177   N  GLY D 130           
SSBOND   1 CYS A  101    CYS A  164                          1555   1555  2.04  
SSBOND   2 CYS A  203    CYS A  259                          1555   1555  2.03  
SSBOND   3 CYS B   25    CYS B   80                          1555   1555  2.03  
SSBOND   4 CYS D   26    CYS D   75                          1555   1555  2.04  
SSBOND   5 CYS D  122    CYS D  174                          1555   1555  2.03  
CISPEP   1 TYR A  209    PRO A  210          0        -4.16                     
CISPEP   2 HIS B   31    PRO B   32          0         3.15                     
CISPEP   3 GLU D   11    PRO D   12          0       -15.73                     
CISPEP   4 GLY D   28    GLY D   29          0        10.18                     
CISPEP   5 GLU D   31    THR D   32          0        -7.24                     
CISPEP   6 ARG N    4    TRP N    5          0         3.97                     
SITE     1 AC1  1 ASN A  86                                                     
CRYST1  231.820  231.820   73.250  90.00  90.00 120.00 P 6 2 2      12          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.004314  0.002491  0.000000        0.00000                         
SCALE2      0.000000  0.004981  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013652        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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