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Database: PDB
Entry: 5KZO
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Original site: 5KZO 
HEADER    TRANSCRIPTION                           25-JUL-16   5KZO              
TITLE     NOTCH1 TRANSMEMBRANE AND ASSOCIATED JUXTAMEMBRANE SEGMENT             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1;                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 1721-1771;                                    
COMPND   5 SYNONYM: HN1,TRANSLOCATION-ASSOCIATED NOTCH PROTEIN TAN-1;           
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: NOTCH1, TAN1;                                                  
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3);                                
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PTRCHIS                               
KEYWDS    NOTCH, MEMBRANE PROTEIN, TRANSCRIPTION                                
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    C.L.DEATHERAGE,Z.LU,B.KRONCKE                                         
REVDAT   3   25-DEC-19 5KZO    1       REMARK                                   
REVDAT   2   27-SEP-17 5KZO    1       REMARK                                   
REVDAT   1   10-MAY-17 5KZO    0                                                
JRNL        AUTH   C.L.DEATHERAGE,Z.LU,B.M.KRONCKE,S.MA,J.A.SMITH,M.W.VOEHLER,  
JRNL        AUTH 2 R.L.MCFEETERS,C.R.SANDERS                                    
JRNL        TITL   STRUCTURAL AND BIOCHEMICAL DIFFERENCES BETWEEN THE NOTCH AND 
JRNL        TITL 2 THE AMYLOID PRECURSOR PROTEIN TRANSMEMBRANE DOMAINS.         
JRNL        REF    SCI ADV                       V.   3 02794 2017              
JRNL        REFN                   ESSN 2375-2548                               
JRNL        PMID   28439555                                                     
JRNL        DOI    10.1126/SCIADV.1602794                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR                                               
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5KZO COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 01-AUG-16.                  
REMARK 100 THE DEPOSITION ID IS D_1000221212.                                   
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 318                                
REMARK 210  PH                             : 5.5                                
REMARK 210  IONIC STRENGTH                 : 65 MM IMIDAZOLE                    
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : 350 UM [U-99% 15N] NOTCH1, 150     
REMARK 210                                   MG/ML DHPC/DMPC BICELLE, 1 MM      
REMARK 210                                   EDTA, 65 MM IMIDAZOLE, 2 MM DTT,   
REMARK 210                                   90% H2O/10% D2O; 500 UM [U-99%     
REMARK 210                                   13C; U-99% 15N] NOTCH1, 150 MG/    
REMARK 210                                   ML DHPC/DMPC, 1 MM EDTA, 65 MM     
REMARK 210                                   IMIDAZOLE, 2 MM DTT, 90% H2O/10%   
REMARK 210                                   D2O; 500 UM [U-99% 13C; U-99%      
REMARK 210                                   15N] NOTCH1, 150 MG/ML DHPC/DMPC   
REMARK 210                                   BICELLE, 100% D2O                  
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC; 3D C(CO)NH; 3D     
REMARK 210                                   H(CCO)NH; 3D HCCH-TOCSY; 3D 1H-    
REMARK 210                                   15N NOESY                          
REMARK 210  SPECTROMETER FIELD STRENGTH    : 900 MHZ; 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : AVANCE III                         
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : SPARKY, TALOS                      
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 2000                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;                   
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                            
REMARK 465   MODELS 1-10                                                        
REMARK 465     RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     GLY A     2                                                      
REMARK 465     HIS A     3                                                      
REMARK 465     HIS A     4                                                      
REMARK 465     HIS A     5                                                      
REMARK 465     HIS A     6                                                      
REMARK 465     HIS A     7                                                      
REMARK 465     HIS A     8                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500  1 PRO A1728   CD    PRO A1728   N      -0.087                       
REMARK 500  1 TYR A1738   CG    TYR A1738   CD2     0.082                       
REMARK 500  6 HIS A1764   CG    HIS A1764   CD2     0.059                       
REMARK 500  6 PHE A1769   CB    PHE A1769   CG      0.102                       
REMARK 500 10 PHE A1736   CZ    PHE A1736   CE2     0.128                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 PHE A1736   CB  -  CG  -  CD2 ANGL. DEV. =  -5.1 DEGREES          
REMARK 500  1 TYR A1738   CB  -  CG  -  CD2 ANGL. DEV. =  -4.6 DEGREES          
REMARK 500  1 PHE A1744   CB  -  CG  -  CD2 ANGL. DEV. =   5.4 DEGREES          
REMARK 500  1 PHE A1744   CB  -  CG  -  CD1 ANGL. DEV. =  -4.6 DEGREES          
REMARK 500  1 CYS A1752   CA  -  CB  -  SG  ANGL. DEV. = -11.3 DEGREES          
REMARK 500  1 ARG A1758   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.2 DEGREES          
REMARK 500  1 ARG A1758   NE  -  CZ  -  NH2 ANGL. DEV. =   4.5 DEGREES          
REMARK 500  1 ARG A1760   NE  -  CZ  -  NH1 ANGL. DEV. =   5.3 DEGREES          
REMARK 500  1 ARG A1761   NE  -  CZ  -  NH1 ANGL. DEV. =  -5.8 DEGREES          
REMARK 500  1 ARG A1762   NE  -  CZ  -  NH1 ANGL. DEV. =  -5.9 DEGREES          
REMARK 500  1 ARG A1762   NE  -  CZ  -  NH2 ANGL. DEV. =   4.6 DEGREES          
REMARK 500  1 PHE A1769   CB  -  CG  -  CD2 ANGL. DEV. =   6.7 DEGREES          
REMARK 500  1 PHE A1769   CB  -  CG  -  CD1 ANGL. DEV. =  -5.6 DEGREES          
REMARK 500  2 PHE A1744   CB  -  CG  -  CD1 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  2 ARG A1758   NE  -  CZ  -  NH2 ANGL. DEV. =   4.1 DEGREES          
REMARK 500  2 ARG A1760   NH1 -  CZ  -  NH2 ANGL. DEV. =  -7.2 DEGREES          
REMARK 500  2 ARG A1760   NE  -  CZ  -  NH2 ANGL. DEV. =   7.5 DEGREES          
REMARK 500  2 ARG A1761   NE  -  CZ  -  NH2 ANGL. DEV. =   6.7 DEGREES          
REMARK 500  2 ARG A1762   NE  -  CZ  -  NH1 ANGL. DEV. =   4.3 DEGREES          
REMARK 500  2 TRP A1768   CD1 -  NE1 -  CE2 ANGL. DEV. =   7.6 DEGREES          
REMARK 500  2 TRP A1768   CZ3 -  CH2 -  CZ2 ANGL. DEV. =  -8.4 DEGREES          
REMARK 500  3 ARG A1758   NE  -  CZ  -  NH2 ANGL. DEV. =   3.3 DEGREES          
REMARK 500  3 ARG A1761   NE  -  CZ  -  NH1 ANGL. DEV. =  -4.0 DEGREES          
REMARK 500  3 ARG A1761   NE  -  CZ  -  NH2 ANGL. DEV. =   4.2 DEGREES          
REMARK 500  3 TRP A1768   N   -  CA  -  CB  ANGL. DEV. =  10.8 DEGREES          
REMARK 500  3 TRP A1768   NE1 -  CE2 -  CD2 ANGL. DEV. =  -8.0 DEGREES          
REMARK 500  3 TRP A1768   CE2 -  CD2 -  CG  ANGL. DEV. =   7.8 DEGREES          
REMARK 500  3 PHE A1769   CB  -  CG  -  CD2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  4 PRO A1729   N   -  CA  -  CB  ANGL. DEV. =   7.9 DEGREES          
REMARK 500  4 TYR A1738   CB  -  CG  -  CD2 ANGL. DEV. =  -5.6 DEGREES          
REMARK 500  4 VAL A1745   CA  -  CB  -  CG2 ANGL. DEV. =   9.2 DEGREES          
REMARK 500  4 ARG A1758   NE  -  CZ  -  NH2 ANGL. DEV. =   3.0 DEGREES          
REMARK 500  4 ARG A1760   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES          
REMARK 500  4 ARG A1761   NE  -  CZ  -  NH1 ANGL. DEV. =   6.0 DEGREES          
REMARK 500  4 ARG A1762   NE  -  CZ  -  NH2 ANGL. DEV. =   5.0 DEGREES          
REMARK 500  4 HIS A1764   CB  -  CG  -  CD2 ANGL. DEV. =  -9.8 DEGREES          
REMARK 500  4 PHE A1769   CB  -  CG  -  CD2 ANGL. DEV. =  -4.5 DEGREES          
REMARK 500  5 VAL A1726   CG1 -  CB  -  CG2 ANGL. DEV. = -10.2 DEGREES          
REMARK 500  5 TYR A1738   CB  -  CG  -  CD1 ANGL. DEV. =  -4.8 DEGREES          
REMARK 500  5 TYR A1738   CG  -  CD1 -  CE1 ANGL. DEV. =  -6.4 DEGREES          
REMARK 500  5 PHE A1748   CB  -  CG  -  CD1 ANGL. DEV. =   4.5 DEGREES          
REMARK 500  5 VAL A1750   CG1 -  CB  -  CG2 ANGL. DEV. = -11.6 DEGREES          
REMARK 500  5 ARG A1758   NH1 -  CZ  -  NH2 ANGL. DEV. =  -9.6 DEGREES          
REMARK 500  5 ARG A1758   NE  -  CZ  -  NH2 ANGL. DEV. =   7.4 DEGREES          
REMARK 500  5 ARG A1760   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500  5 ARG A1761   CD  -  NE  -  CZ  ANGL. DEV. =   8.4 DEGREES          
REMARK 500  5 ARG A1762   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  5 TRP A1768   CD1 -  NE1 -  CE2 ANGL. DEV. =   5.8 DEGREES          
REMARK 500  6 VAL A1726   CA  -  CB  -  CG2 ANGL. DEV. =   9.3 DEGREES          
REMARK 500  6 TYR A1738   CB  -  CG  -  CD2 ANGL. DEV. =  -4.9 DEGREES          
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     114 ANGLE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 LYS A1759     -102.47   -158.35                                   
REMARK 500  1 ARG A1760      -51.44     53.23                                   
REMARK 500  1 ARG A1761       56.88    -67.40                                   
REMARK 500  1 GLN A1763     -156.42     53.91                                   
REMARK 500  1 HIS A1764      -93.99     36.03                                   
REMARK 500  1 GLN A1766        5.15   -150.70                                   
REMARK 500  1 TRP A1768      -65.42     72.69                                   
REMARK 500  2 GLN A1722      -81.89   -109.18                                   
REMARK 500  2 SER A1723      115.00   -166.23                                   
REMARK 500  2 GLU A1724       -0.67     65.71                                   
REMARK 500  2 VAL A1726       11.38   -140.28                                   
REMARK 500  2 PRO A1729      156.84    -46.71                                   
REMARK 500  2 VAL A1739      -63.31    -99.24                                   
REMARK 500  2 LYS A1759      125.05    162.13                                   
REMARK 500  2 ARG A1760       -6.32   -170.33                                   
REMARK 500  2 ARG A1762     -103.93     55.24                                   
REMARK 500  2 GLN A1763      -27.71     62.89                                   
REMARK 500  2 HIS A1764      -98.43    -78.07                                   
REMARK 500  2 GLN A1766       -3.69   -160.09                                   
REMARK 500  2 TRP A1768      -54.52     55.76                                   
REMARK 500  3 GLU A1724      -35.86     61.70                                   
REMARK 500  3 GLN A1763      149.36     66.91                                   
REMARK 500  3 HIS A1764      -63.33     63.10                                   
REMARK 500  3 GLN A1766       -7.91   -149.15                                   
REMARK 500  3 TRP A1768      -64.28     71.51                                   
REMARK 500  4 LEU A1734      -71.70    -75.90                                   
REMARK 500  4 LYS A1759      -99.41   -150.93                                   
REMARK 500  4 ARG A1760      -45.65     58.44                                   
REMARK 500  4 ARG A1761      -73.89   -142.45                                   
REMARK 500  4 HIS A1764      -49.72     64.99                                   
REMARK 500  4 GLN A1766        4.80   -166.12                                   
REMARK 500  4 TRP A1768      -48.88     59.31                                   
REMARK 500  4 PRO A1770       43.09    -87.94                                   
REMARK 500  5 LEU A1734      -77.29    -80.99                                   
REMARK 500  5 LYS A1759      -69.42   -177.71                                   
REMARK 500  5 ARG A1760      -42.23     50.59                                   
REMARK 500  5 ARG A1761     -100.43   -108.60                                   
REMARK 500  5 GLN A1763       49.79    -81.09                                   
REMARK 500  5 HIS A1764     -176.32     64.21                                   
REMARK 500  5 GLN A1766       15.76   -150.17                                   
REMARK 500  5 LEU A1767       66.05   -101.18                                   
REMARK 500  5 TRP A1768      -63.51     76.80                                   
REMARK 500  6 GLN A1722       -5.14   -142.45                                   
REMARK 500  6 GLU A1724        4.11     52.62                                   
REMARK 500  6 LYS A1759     -108.99   -176.88                                   
REMARK 500  6 ARG A1760      -12.08     61.07                                   
REMARK 500  6 ARG A1761       89.27     63.54                                   
REMARK 500  6 GLN A1763      156.31     67.51                                   
REMARK 500  6 HIS A1764      -17.77     58.75                                   
REMARK 500  6 LEU A1767       66.03   -107.64                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      85 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 TYR A1738         0.12    SIDE CHAIN                              
REMARK 500  2 TYR A1738         0.13    SIDE CHAIN                              
REMARK 500  2 ARG A1761         0.09    SIDE CHAIN                              
REMARK 500  6 TYR A1738         0.09    SIDE CHAIN                              
REMARK 500  6 PHE A1744         0.09    SIDE CHAIN                              
REMARK 500  6 ARG A1760         0.15    SIDE CHAIN                              
REMARK 500  6 ARG A1762         0.13    SIDE CHAIN                              
REMARK 500  8 PHE A1744         0.07    SIDE CHAIN                              
REMARK 500  9 HIS A1735         0.12    SIDE CHAIN                              
REMARK 500  9 PHE A1744         0.10    SIDE CHAIN                              
REMARK 500 10 PHE A1749         0.07    SIDE CHAIN                              
REMARK 500 10 HIS A1764         0.06    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY                                       
REMARK 500                                                                      
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY                       
REMARK 500 TORSION ANGLE, C(I) - CA(I) - N(I+1) - O(I), GREATER                 
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;                     
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;                            
REMARK 500 I=INSERTION CODE).                                                   
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        ANGLE                                           
REMARK 500  7 PHE A1749        -12.52                                           
REMARK 500  7 TRP A1768        -10.01                                           
REMARK 500  8 TRP A1768        -10.51                                           
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 30147   RELATED DB: BMRB                                 
REMARK 900 RELATED ID: 26565   RELATED DB: BMRB                                 
DBREF  5KZO A 1721  1771  UNP    P46531   NOTC1_HUMAN   1721   1771             
SEQADV 5KZO MET A    1  UNP  P46531              INITIATING METHIONINE          
SEQADV 5KZO GLY A    2  UNP  P46531              EXPRESSION TAG                 
SEQADV 5KZO HIS A    3  UNP  P46531              EXPRESSION TAG                 
SEQADV 5KZO HIS A    4  UNP  P46531              EXPRESSION TAG                 
SEQADV 5KZO HIS A    5  UNP  P46531              EXPRESSION TAG                 
SEQADV 5KZO HIS A    6  UNP  P46531              EXPRESSION TAG                 
SEQADV 5KZO HIS A    7  UNP  P46531              EXPRESSION TAG                 
SEQADV 5KZO HIS A    8  UNP  P46531              EXPRESSION TAG                 
SEQRES   1 A   59  MET GLY HIS HIS HIS HIS HIS HIS VAL GLN SER GLU THR          
SEQRES   2 A   59  VAL GLU PRO PRO PRO PRO ALA GLN LEU HIS PHE MET TYR          
SEQRES   3 A   59  VAL ALA ALA ALA ALA PHE VAL LEU LEU PHE PHE VAL GLY          
SEQRES   4 A   59  CYS GLY VAL LEU LEU SER ARG LYS ARG ARG ARG GLN HIS          
SEQRES   5 A   59  GLY GLN LEU TRP PHE PRO GLU                                  
HELIX    1 AA1 PRO A 1731  SER A 1757  1                                  27    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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