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Database: PDB
Entry: 5LGG
LinkDB: 5LGG
Original site: 5LGG 
HEADER    CELL CYCLE                              07-JUL-16   5LGG              
TITLE     THE N-TERMINAL WD40 DOMAIN OF APC1 (ANAPHASE PROMOTING COMPLEX SUBUNIT
TITLE    2 1)                                                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ANAPHASE-PROMOTING COMPLEX SUBUNIT 1,ANAPHASE-PROMOTING    
COMPND   3 COMPLEX SUBUNIT 1,ANAPHASE-PROMOTING COMPLEX SUBUNIT 1,ANAPHASE-     
COMPND   4 PROMOTING COMPLEX SUBUNIT 1;                                         
COMPND   5 CHAIN: A;                                                            
COMPND   6 SYNONYM: APC1,CYCLOSOME SUBUNIT 1,MITOTIC CHECKPOINT REGULATOR,      
COMPND   7 TESTIS-SPECIFIC GENE 24 PROTEIN,APC1,CYCLOSOME SUBUNIT 1,MITOTIC     
COMPND   8 CHECKPOINT REGULATOR,TESTIS-SPECIFIC GENE 24 PROTEIN,APC1,CYCLOSOME  
COMPND   9 SUBUNIT 1,MITOTIC CHECKPOINT REGULATOR,TESTIS-SPECIFIC GENE 24       
COMPND  10 PROTEIN,APC1,CYCLOSOME SUBUNIT 1,MITOTIC CHECKPOINT REGULATOR,TESTIS-
COMPND  11 SPECIFIC GENE 24 PROTEIN;                                            
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ANAPC1, TSG24;                                                 
SOURCE   6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;                            
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 7108                                        
KEYWDS    APC/C, CELL CYCLE, WD40                                               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Q.LI,S.AIBARA,D.BARFORD                                               
REVDAT   3   30-AUG-17 5LGG    1       REMARK                                   
REVDAT   2   26-OCT-16 5LGG    1       COMPND SOURCE DBREF                      
REVDAT   1   05-OCT-16 5LGG    0                                                
JRNL        AUTH   Q.LI,L.CHANG,S.AIBARA,J.YANG,Z.ZHANG,D.BARFORD               
JRNL        TITL   WD40 DOMAIN OF APC1 IS CRITICAL FOR THE COACTIVATOR-INDUCED  
JRNL        TITL 2 ALLOSTERIC TRANSITION THAT STIMULATES APC/C CATALYTIC        
JRNL        TITL 3 ACTIVITY.                                                    
JRNL        REF    PROC.NATL.ACAD.SCI.USA        V. 113 10547 2016              
JRNL        REFN                   ESSN 1091-6490                               
JRNL        PMID   27601667                                                     
JRNL        DOI    10.1073/PNAS.1607147113                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.15 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX DEV_1810                                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.15                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 40.13                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 25388                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.181                           
REMARK   3   R VALUE            (WORKING SET) : 0.179                           
REMARK   3   FREE R VALUE                     : 0.227                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.910                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1246                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 40.1376 -  4.4704    0.99     2859   161  0.1568 0.1915        
REMARK   3     2  4.4704 -  3.5490    1.00     2770   133  0.1469 0.1844        
REMARK   3     3  3.5490 -  3.1005    1.00     2741   150  0.1703 0.2469        
REMARK   3     4  3.1005 -  2.8171    1.00     2734   135  0.1978 0.2181        
REMARK   3     5  2.8171 -  2.6153    1.00     2715   139  0.2063 0.2770        
REMARK   3     6  2.6153 -  2.4611    1.00     2719   150  0.2130 0.2723        
REMARK   3     7  2.4611 -  2.3379    1.00     2690   138  0.2365 0.2952        
REMARK   3     8  2.3379 -  2.2361    0.96     2610   121  0.2569 0.3582        
REMARK   3     9  2.2361 -  2.1500    0.85     2304   119  0.2693 0.3009        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.230            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.870           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.009           3049                                  
REMARK   3   ANGLE     :  1.198           4129                                  
REMARK   3   CHIRALITY :  0.048            475                                  
REMARK   3   PLANARITY :  0.005            522                                  
REMARK   3   DIHEDRAL  : 15.040           1124                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 7                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 2 THROUGH 22 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -10.9525 -27.0090  16.5000              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3740 T22:   0.3763                                     
REMARK   3      T33:   0.2897 T12:  -0.0781                                     
REMARK   3      T13:  -0.0051 T23:   0.0573                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.1803 L22:   2.3207                                     
REMARK   3      L33:   5.1637 L12:   0.1737                                     
REMARK   3      L13:  -0.1675 L23:  -0.9675                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4478 S12:  -0.5784 S13:   0.1075                       
REMARK   3      S21:   0.7419 S22:  -0.4225 S23:   0.1120                       
REMARK   3      S31:   0.1536 S32:  -0.1168 S33:  -0.0867                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 23 THROUGH 89 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -19.7445 -37.1466  -8.2891              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4664 T22:   0.4600                                     
REMARK   3      T33:   0.4677 T12:   0.1439                                     
REMARK   3      T13:  -0.0554 T23:  -0.0053                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5498 L22:   3.3976                                     
REMARK   3      L33:   3.3116 L12:  -0.0262                                     
REMARK   3      L13:   0.5741 L23:  -0.3307                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2478 S12:  -0.6774 S13:  -0.8695                       
REMARK   3      S21:  -0.0379 S22:  -0.1132 S23:   0.3070                       
REMARK   3      S31:   0.4641 S32:   0.1980 S33:   0.0119                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 90 THROUGH 154 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -26.0060 -31.0187 -15.4675              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3202 T22:   0.3066                                     
REMARK   3      T33:   0.2853 T12:  -0.0070                                     
REMARK   3      T13:  -0.0191 T23:  -0.0270                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.6754 L22:   2.4946                                     
REMARK   3      L33:   4.4371 L12:  -0.7932                                     
REMARK   3      L13:  -0.2794 L23:   0.3886                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2013 S12:   0.1516 S13:  -0.1019                       
REMARK   3      S21:   0.1007 S22:  -0.0847 S23:   0.1688                       
REMARK   3      S31:   0.3867 S32:   0.3208 S33:  -0.1163                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 155 THROUGH 260 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -20.8931 -15.3523 -24.5126              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4363 T22:   0.4287                                     
REMARK   3      T33:   0.3462 T12:  -0.1008                                     
REMARK   3      T13:  -0.0075 T23:   0.1046                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.6043 L22:   1.3847                                     
REMARK   3      L33:   4.0108 L12:   0.1673                                     
REMARK   3      L13:   1.3889 L23:   1.2009                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1660 S12:   0.5891 S13:   0.3336                       
REMARK   3      S21:  -0.3717 S22:   0.0183 S23:  -0.1955                       
REMARK   3      S31:  -0.7029 S32:   0.6473 S33:   0.0742                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 261 THROUGH 439 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -19.2480  -7.4507 -13.8021              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6397 T22:   0.2526                                     
REMARK   3      T33:   0.4463 T12:  -0.1262                                     
REMARK   3      T13:  -0.0971 T23:   0.0855                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7915 L22:   1.0245                                     
REMARK   3      L33:   2.2726 L12:  -0.3389                                     
REMARK   3      L13:   0.5849 L23:  -0.2919                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4050 S12:   0.2692 S13:   0.4072                       
REMARK   3      S21:  -0.1835 S22:   0.0563 S23:  -0.0237                       
REMARK   3      S31:  -1.2308 S32:   0.3950 S33:   0.1251                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 440 THROUGH 511 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -14.2753 -14.1566   1.8658              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2986 T22:   0.2513                                     
REMARK   3      T33:   0.3315 T12:  -0.0357                                     
REMARK   3      T13:  -0.0224 T23:   0.0215                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5776 L22:   3.7453                                     
REMARK   3      L33:   5.0690 L12:  -1.6106                                     
REMARK   3      L13:   1.2861 L23:  -0.7247                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2129 S12:   0.0385 S13:   0.0907                       
REMARK   3      S21:   0.0937 S22:   0.0437 S23:   0.0097                       
REMARK   3      S31:  -0.7411 S32:   0.1629 S33:   0.1569                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 512 THROUGH 613 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -7.6163 -30.6272   3.9368              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2654 T22:   0.3078                                     
REMARK   3      T33:   0.2955 T12:   0.0593                                     
REMARK   3      T13:  -0.0199 T23:  -0.0662                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.3199 L22:   1.3664                                     
REMARK   3      L33:   3.0218 L12:  -0.2560                                     
REMARK   3      L13:   2.5769 L23:  -0.7801                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4722 S12:   0.3091 S13:  -0.2682                       
REMARK   3      S21:  -0.1834 S22:  -0.1723 S23:  -0.0548                       
REMARK   3      S31:   0.5062 S32:   0.3566 S33:  -0.2610                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5LGG COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 07-JUL-16.                  
REMARK 100 THE DEPOSITION ID IS D_1200000681.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 24-NOV-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : DIAMOND                            
REMARK 200  BEAMLINE                       : I02                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9795                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M-F               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS                            
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25408                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.150                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 40.131                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.8                               
REMARK 200  DATA REDUNDANCY                : 4.800                              
REMARK 200  R MERGE                    (I) : 0.05931                            
REMARK 200  R SYM                      (I) : 0.06606                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 16.7600                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.15                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.22                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 84.7                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.50                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.43300                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.010                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 4UI9                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 49.85                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.45                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M CITRIC ACID, PH5.0, 2M NACL,       
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       50.21800            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       50.21800            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       41.31650            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       56.63100            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       41.31650            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       56.63100            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       50.21800            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       41.31650            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       56.63100            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       50.21800            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       41.31650            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       56.63100            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC                  
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 0 ANGSTROM**2                             
REMARK 350 SURFACE AREA OF THE COMPLEX: 20880 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 0.0 KCAL/MOL                          
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     HIS A    61                                                      
REMARK 465     PRO A    62                                                      
REMARK 465     ASN A    63                                                      
REMARK 465     GLY A    64                                                      
REMARK 465     SER A    65                                                      
REMARK 465     ALA A    66                                                      
REMARK 465     GLY A    67                                                      
REMARK 465     SER A    68                                                      
REMARK 465     ALA A    69                                                      
REMARK 465     SER A   195                                                      
REMARK 465     HIS A   196                                                      
REMARK 465     GLU A   197                                                      
REMARK 465     VAL A   198                                                      
REMARK 465     PRO A   199                                                      
REMARK 465     PRO A   200                                                      
REMARK 465     GLY A   201                                                      
REMARK 465     SER A   202                                                      
REMARK 465     GLY A   232                                                      
REMARK 465     SER A   233                                                      
REMARK 465     SER A   234                                                      
REMARK 465     VAL A   375                                                      
REMARK 465     VAL A   376                                                      
REMARK 465     LEU A   377                                                      
REMARK 465     LYS A   378                                                      
REMARK 465     PHE A   379                                                      
REMARK 465     SER A   380                                                      
REMARK 465     GLU A   381                                                      
REMARK 465     GLN A   382                                                      
REMARK 465     GLY A   383                                                      
REMARK 465     GLY A   384                                                      
REMARK 465     THR A   385                                                      
REMARK 465     PRO A   386                                                      
REMARK 465     GLN A   387                                                      
REMARK 465     ASN A   388                                                      
REMARK 465     VAL A   389                                                      
REMARK 465     ALA A   390                                                      
REMARK 465     THR A   391                                                      
REMARK 465     SER A   392                                                      
REMARK 465     SER A   393                                                      
REMARK 465     SER A   394                                                      
REMARK 465     LEU A   395                                                      
REMARK 465     THR A   396                                                      
REMARK 465     ALA A   397                                                      
REMARK 465     HIS A   398                                                      
REMARK 465     LEU A   399                                                      
REMARK 465     ARG A   400                                                      
REMARK 465     GLY A   401                                                      
REMARK 465     SER A   402                                                      
REMARK 465     THR A   418                                                      
REMARK 465     ASN A   419                                                      
REMARK 465     ILE A   420                                                      
REMARK 465     ARG A   421                                                      
REMARK 480                                                                      
REMARK 480 ZERO OCCUPANCY ATOM                                                  
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO                  
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS                
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;              
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):         
REMARK 480   M RES C SSEQI ATOMS                                                
REMARK 480     GLU A   19   CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HOH A   714     O    HOH A   825              2.02            
REMARK 500   O    HOH A   776     O    HOH A   863              2.05            
REMARK 500   O    HOH A   746     O    HOH A   841              2.06            
REMARK 500   O    HOH A   710     O    HOH A   792              2.10            
REMARK 500   OD1  ASP A   462     OG1  THR A   464              2.12            
REMARK 500   NE   ARG A    15     O    HOH A   701              2.13            
REMARK 500   OE1  GLU A    19     O    HOH A   702              2.16            
REMARK 500   OG1  THR A   596     O    HOH A   703              2.17            
REMARK 500   NH1  ARG A   191     O    PRO A   208              2.18            
REMARK 500   OG1  THR A   596     O    HOH A   704              2.18            
REMARK 500   O    HOH A   723     O    HOH A   800              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   OG   SER A   172     O    HOH A   701     6444     2.17            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG A   8      -49.15   -133.51                                   
REMARK 500    GLN A 125      143.57   -170.23                                   
REMARK 500    LYS A 463       14.08     59.85                                   
REMARK 500    ALA A 516      156.95    -49.23                                   
REMARK 500    HIS A 592     -118.58     53.72                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  5LGG A    1    63  UNP    Q9H1A4   APC1_HUMAN       1     33             
DBREF  5LGG A   70   400  UNP    Q9H1A4   APC1_HUMAN      70    306             
DBREF  5LGG A  403   522  UNP    Q9H1A4   APC1_HUMAN     403    522             
DBREF  5LGG A  581   613  UNP    Q9H1A4   APC1_HUMAN     581    613             
SEQADV 5LGG GLY A   64  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG SER A   65  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG ALA A   66  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG GLY A   67  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG SER A   68  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG ALA A   69  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG GLY A  401  UNP  Q9H1A4              LINKER                         
SEQADV 5LGG SER A  402  UNP  Q9H1A4              LINKER                         
SEQRES   1 A  431  MET SER ASN PHE TYR GLU GLU ARG THR THR MET ILE ALA          
SEQRES   2 A  431  ALA ARG ASP LEU GLN GLU PHE VAL PRO PHE GLY ARG ASP          
SEQRES   3 A  431  HIS CYS LYS HIS HIS PRO ASN GLY SER ALA GLY SER ALA          
SEQRES   4 A  431  GLN LYS GLU SER TRP GLN LEU ARG LYS GLY VAL SER GLU          
SEQRES   5 A  431  ILE GLY GLU ASP VAL ASP TYR ASP GLU GLU LEU TYR VAL          
SEQRES   6 A  431  ALA GLY ASN MET VAL ILE TRP SER LYS GLY SER LYS SER          
SEQRES   7 A  431  GLN ALA LEU ALA VAL TYR LYS ALA PHE THR VAL ASP SER          
SEQRES   8 A  431  PRO VAL GLN GLN ALA LEU TRP CYS ASP PHE ILE ILE SER          
SEQRES   9 A  431  GLN ASP LYS SER GLU LYS ALA TYR SER SER ASN GLU VAL          
SEQRES  10 A  431  GLU LYS CYS ILE CYS ILE LEU GLN SER SER CYS ILE ASN          
SEQRES  11 A  431  MET HIS SER ILE GLU GLY LYS ASP TYR ILE ALA SER LEU          
SEQRES  12 A  431  PRO PHE GLN VAL ALA ASN VAL TRP PRO THR LYS TYR GLY          
SEQRES  13 A  431  LEU LEU PHE GLU ARG SER ALA SER SER HIS GLU VAL PRO          
SEQRES  14 A  431  PRO GLY SER PRO ARG GLU PRO LEU PRO THR MET PHE SER          
SEQRES  15 A  431  MET LEU HIS PRO LEU ASP GLU ILE THR PRO LEU VAL CYS          
SEQRES  16 A  431  LYS SER GLY SER LEU PHE GLY SER SER ARG VAL GLN TYR          
SEQRES  17 A  431  VAL VAL ASP HIS ALA MET LYS ILE VAL PHE LEU ASN THR          
SEQRES  18 A  431  ASP PRO SER ILE VAL MET THR TYR ASP ALA VAL GLN ASN          
SEQRES  19 A  431  VAL HIS SER VAL TRP THR LEU ARG ARG VAL LYS SER GLU          
SEQRES  20 A  431  GLU GLU ASN VAL VAL LEU LYS PHE SER GLU GLN GLY GLY          
SEQRES  21 A  431  THR PRO GLN ASN VAL ALA THR SER SER SER LEU THR ALA          
SEQRES  22 A  431  HIS LEU ARG GLY SER ILE VAL PRO GLU LEU CYS ILE ASP          
SEQRES  23 A  431  HIS LEU TRP THR GLU THR ILE THR ASN ILE ARG GLU LYS          
SEQRES  24 A  431  ASN SER GLN ALA SER LYS VAL PHE ILE THR SER ASP LEU          
SEQRES  25 A  431  CYS GLY GLN LYS PHE LEU CYS PHE LEU VAL GLU SER GLN          
SEQRES  26 A  431  LEU GLN LEU ARG CYS VAL LYS PHE GLN GLU SER ASN ASP          
SEQRES  27 A  431  LYS THR GLN LEU ILE PHE GLY SER VAL THR ASN ILE PRO          
SEQRES  28 A  431  ALA LYS ASP ALA ALA PRO VAL GLU LYS ILE ASP THR MET          
SEQRES  29 A  431  LEU VAL LEU GLU GLY SER GLY ASN LEU VAL LEU TYR THR          
SEQRES  30 A  431  GLY VAL VAL ARG VAL GLY LYS VAL PHE ILE PRO GLY LEU          
SEQRES  31 A  431  PRO ALA PRO SER LEU THR MET SER GLY THR TYR ILE HIS          
SEQRES  32 A  431  SER ILE ARG ASP PRO VAL HIS ASN ARG VAL THR LEU GLU          
SEQRES  33 A  431  LEU SER ASN GLY SER MET VAL ARG ILE THR ILE PRO GLU          
SEQRES  34 A  431  ILE ALA                                                      
FORMUL   2  HOH   *167(H2 O)                                                    
HELIX    1 AA1 PRO A   22  CYS A   28  5                                   7    
HELIX    2 AA2 GLU A  447  GLN A  449  5                                   3    
HELIX    3 AA3 GLU A  483  ASP A  486  5                                   4    
HELIX    4 AA4 ALA A  516  THR A  520  5                                   5    
SHEET    1 AA1 5 MET A  11  ALA A  13  0                                        
SHEET    2 AA1 5 VAL A 504  PHE A 510  1  O  PHE A 510   N  ILE A  12           
SHEET    3 AA1 5 LEU A 497  THR A 501 -1  N  LEU A 499   O  GLY A 507           
SHEET    4 AA1 5 THR A 487  LEU A 491 -1  N  MET A 488   O  TYR A 500           
SHEET    5 AA1 5 ASP A 478  VAL A 482 -1  N  ASP A 478   O  LEU A 491           
SHEET    1 AA2 4 ARG A  15  GLU A  19  0                                        
SHEET    2 AA2 4 MET A 604  THR A 608 -1  O  ARG A 606   N  GLN A  18           
SHEET    3 AA2 4 ARG A 594  LEU A 599 -1  N  LEU A 597   O  VAL A 605           
SHEET    4 AA2 4 ILE A 584  VAL A 591 -1  N  SER A 586   O  GLU A 598           
SHEET    1 AA3 4 GLU A  72  LYS A  78  0                                        
SHEET    2 AA3 4 TYR A  89  ALA A  96 -1  O  VAL A  95   N  SER A  73           
SHEET    3 AA3 4 MET A  99  SER A 106 -1  O  GLY A 105   N  ASP A  90           
SHEET    4 AA3 4 VAL A 113  THR A 118 -1  O  LYS A 115   N  TRP A 102           
SHEET    1 AA4 4 GLN A 125  SER A 134  0                                        
SHEET    2 AA4 4 GLU A 146  LEU A 154 -1  O  CYS A 152   N  LEU A 127           
SHEET    3 AA4 4 ILE A 159  SER A 163 -1  O  ASN A 160   N  ILE A 153           
SHEET    4 AA4 4 ASP A 168  ALA A 171 -1  O  TYR A 169   N  MET A 161           
SHEET    1 AA5 4 VAL A 177  THR A 183  0                                        
SHEET    2 AA5 4 GLY A 186  ARG A 191 -1  O  GLU A 190   N  ALA A 178           
SHEET    3 AA5 4 MET A 210  MET A 213 -1  O  PHE A 211   N  PHE A 189           
SHEET    4 AA5 4 THR A 221  PRO A 222 -1  O  THR A 221   N  SER A 212           
SHEET    1 AA6 6 GLN A 237  TYR A 238  0                                        
SHEET    2 AA6 6 VAL A 224  LYS A 226 -1  N  CYS A 225   O  GLN A 237           
SHEET    3 AA6 6 LEU A 407  THR A 414  1  O  ILE A 409   N  LYS A 226           
SHEET    4 AA6 6 VAL A 265  ARG A 273 -1  N  VAL A 268   O  TRP A 413           
SHEET    5 AA6 6 ILE A 255  ASP A 260 -1  N  THR A 258   O  SER A 267           
SHEET    6 AA6 6 MET A 244  ASN A 250 -1  N  VAL A 247   O  MET A 257           
SHEET    1 AA7 4 PHE A 431  SER A 434  0                                        
SHEET    2 AA7 4 LYS A 440  VAL A 446 -1  O  CYS A 443   N  PHE A 431           
SHEET    3 AA7 4 GLN A 451  GLU A 459 -1  O  GLN A 451   N  VAL A 446           
SHEET    4 AA7 4 LEU A 466  PRO A 475 -1  O  ILE A 474   N  LEU A 452           
CISPEP   1 ASP A  252    PRO A  253          0         2.04                     
CRYST1   82.633  113.262  100.436  90.00  90.00  90.00 C 2 2 21      8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.012102  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.008829  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.009957        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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