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Database: PDB
Entry: 5T01
LinkDB: 5T01
Original site: 5T01 
HEADER    TRANSCRIPTION REGULATOR/DNA             15-AUG-16   5T01              
TITLE     HUMAN C-JUN DNA BINDING DOMAIN HOMODIMER IN COMPLEX WITH METHYLATED   
TITLE    2 DNA                                                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DNA (5'-                                                   
COMPND   3 D(P*CP*TP*CP*CP*TP*AP*TP*GP*AP*CP*TP*CP*GP*TP*CP*CP*AP*T)-3');       
COMPND   4 CHAIN: C;                                                            
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: DNA (5'-D(*AP*AP*TP*GP*GP*AP*(5CM)                         
COMPND   8 P*GP*AP*GP*TP*CP*AP*TP*AP*GP*GP*AP*G)-3');                           
COMPND   9 CHAIN: D;                                                            
COMPND  10 ENGINEERED: YES;                                                     
COMPND  11 MOL_ID: 3;                                                           
COMPND  12 MOLECULE: TRANSCRIPTION FACTOR AP-1;                                 
COMPND  13 CHAIN: A, B;                                                         
COMPND  14 FRAGMENT: DNA BINDING DOMAIN (UNP RESIDUES 254-315);                 
COMPND  15 SYNONYM: ACTIVATOR PROTEIN 1,AP1,PROTO-ONCOGENE C-JUN,V-JUN AVIAN    
COMPND  16 SARCOMA VIRUS 17 ONCOGENE HOMOLOG,P39;                               
COMPND  17 ENGINEERED: YES;                                                     
COMPND  18 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE   4 ORGANISM_TAXID: 32630;                                               
SOURCE   5 MOL_ID: 2;                                                           
SOURCE   6 SYNTHETIC: YES;                                                      
SOURCE   7 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE   8 ORGANISM_TAXID: 32630;                                               
SOURCE   9 MOL_ID: 3;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 GENE: JUN;                                                           
SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    ZTA, ZEBRA, BZLF-1, AP-1, EPSTEIN-BARR VIRUS, EBV, 5-METHYLCYTOSINE,  
KEYWDS   2 5MC, DNA METHYLATION, TRANSCRIPTION FACTOR, BASIC LEUCINE-ZIPPER,    
KEYWDS   3 BZIP, TRANSCRIPTION REGULATOR-DNA COMPLEX                            
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    S.HONG,J.R.HORTON,X.CHENG                                             
REVDAT   3   27-SEP-17 5T01    1       REMARK                                   
REVDAT   2   03-MAY-17 5T01    1       JRNL                                     
REVDAT   1   01-MAR-17 5T01    0                                                
JRNL        AUTH   S.HONG,D.WANG,J.R.HORTON,X.ZHANG,S.H.SPECK,R.M.BLUMENTHAL,   
JRNL        AUTH 2 X.CHENG                                                      
JRNL        TITL   METHYL-DEPENDENT AND SPATIAL-SPECIFIC DNA RECOGNITION BY THE 
JRNL        TITL 2 ORTHOLOGOUS TRANSCRIPTION FACTORS HUMAN AP-1 AND             
JRNL        TITL 3 EPSTEIN-BARR VIRUS ZTA.                                      
JRNL        REF    NUCLEIC ACIDS RES.            V.  45  2503 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   28158710                                                     
JRNL        DOI    10.1093/NAR/GKX057                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.89 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.9_1692                                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.89                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 27.75                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.0                           
REMARK   3   NUMBER OF REFLECTIONS             : 23712                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.195                           
REMARK   3   R VALUE            (WORKING SET) : 0.193                           
REMARK   3   FREE R VALUE                     : 0.227                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.010                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1187                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 27.7531 -  3.7763    0.99     2934   155  0.1496 0.1767        
REMARK   3     2  3.7763 -  2.9985    1.00     2875   151  0.1798 0.2292        
REMARK   3     3  2.9985 -  2.6198    1.00     2875   152  0.2336 0.2497        
REMARK   3     4  2.6198 -  2.3804    1.00     2849   150  0.2380 0.2734        
REMARK   3     5  2.3804 -  2.2099    1.00     2872   151  0.2248 0.2851        
REMARK   3     6  2.2099 -  2.0797    1.00     2858   150  0.2403 0.2835        
REMARK   3     7  2.0797 -  1.9755    0.98     2777   147  0.2695 0.2908        
REMARK   3     8  1.9755 -  1.8896    0.87     2485   131  0.2980 0.3226        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.240            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.090           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.014           1861                                  
REMARK   3   ANGLE     :  1.374           2638                                  
REMARK   3   CHIRALITY :  0.058            296                                  
REMARK   3   PLANARITY :  0.007            205                                  
REMARK   3   DIHEDRAL  : 23.914            802                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 1                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ALL                                                    
REMARK   3    ORIGIN FOR THE GROUP (A): -24.4193  16.7252  20.1421              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3690 T22:   0.3422                                     
REMARK   3      T33:   0.3577 T12:  -0.0006                                     
REMARK   3      T13:  -0.0670 T23:   0.0003                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4259 L22:   0.3155                                     
REMARK   3      L33:   0.3373 L12:   0.3658                                     
REMARK   3      L13:  -0.0501 L23:   0.6462                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0810 S12:  -0.0335 S13:  -0.0295                       
REMARK   3      S21:   0.2442 S22:  -0.0105 S23:  -0.2212                       
REMARK   3      S31:   0.0741 S32:   0.1651 S33:   0.0061                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5T01 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 25-AUG-16.                  
REMARK 100 THE DEPOSITION ID IS D_1000223389.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-FEB-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 22-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000                              
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : RAYONIX MX300-HS                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 23735                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.890                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 35.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.5                               
REMARK 200  DATA REDUNDANCY                : 6.900                              
REMARK 200  R MERGE                    (I) : 0.08300                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 20.3000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.89                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.96                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 90.1                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.20                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.80000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.630                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: 1FOS                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 56.25                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.81                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.05 M CITRIC ACID 0.05 M BIS-TRIS       
REMARK 280  PROPANE 16% PEG3350, PH 5.0, VAPOR DIFFUSION, HANGING DROP,         
REMARK 280  TEMPERATURE 289K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       79.43550            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       21.24500            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       79.43550            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       21.24500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7000 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15190 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -42.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D, A, B                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465      DT C     0                                                      
REMARK 465     ASN A   314                                                      
REMARK 465     HIS A   315                                                      
REMARK 465     ASN B   314                                                      
REMARK 465     HIS B   315                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS B 311    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500     DT C  11   O3'    DT C  11   C3'    -0.056                       
REMARK 500     DC C  15   O3'    DC C  15   C3'    -0.037                       
REMARK 500     DA D  31   O3'    DA D  31   C3'    -0.061                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DT C  18   O4' -  C1' -  N1  ANGL. DEV. =   1.9 DEGREES          
REMARK 500     DG D  41   O4' -  C1' -  N9  ANGL. DEV. =   2.3 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5SZX   RELATED DB: PDB                                   
DBREF  5T01 C    0    18  PDB    5T01     5T01             0     18             
DBREF  5T01 D   23    41  PDB    5T01     5T01            23     41             
DBREF  5T01 A  254   315  UNP    P05412   JUN_HUMAN      254    315             
DBREF  5T01 B  254   315  UNP    P05412   JUN_HUMAN      254    315             
SEQADV 5T01 HIS A  252  UNP  P05412              EXPRESSION TAG                 
SEQADV 5T01 MET A  253  UNP  P05412              EXPRESSION TAG                 
SEQADV 5T01 SER A  269  UNP  P05412    CYS   269 ENGINEERED MUTATION            
SEQADV 5T01 HIS B  252  UNP  P05412              EXPRESSION TAG                 
SEQADV 5T01 MET B  253  UNP  P05412              EXPRESSION TAG                 
SEQADV 5T01 SER B  269  UNP  P05412    CYS   269 ENGINEERED MUTATION            
SEQRES   1 C   19   DT  DC  DT  DC  DC  DT  DA  DT  DG  DA  DC  DT  DC          
SEQRES   2 C   19   DG  DT  DC  DC  DA  DT                                      
SEQRES   1 D   19   DA  DA  DT  DG  DG  DA 5CM  DG  DA  DG  DT  DC  DA          
SEQRES   2 D   19   DT  DA  DG  DG  DA  DG                                      
SEQRES   1 A   64  HIS MET LYS ALA GLU ARG LYS ARG MET ARG ASN ARG ILE          
SEQRES   2 A   64  ALA ALA SER LYS SER ARG LYS ARG LYS LEU GLU ARG ILE          
SEQRES   3 A   64  ALA ARG LEU GLU GLU LYS VAL LYS THR LEU LYS ALA GLN          
SEQRES   4 A   64  ASN SER GLU LEU ALA SER THR ALA ASN MET LEU ARG GLU          
SEQRES   5 A   64  GLN VAL ALA GLN LEU LYS GLN LYS VAL MET ASN HIS              
SEQRES   1 B   64  HIS MET LYS ALA GLU ARG LYS ARG MET ARG ASN ARG ILE          
SEQRES   2 B   64  ALA ALA SER LYS SER ARG LYS ARG LYS LEU GLU ARG ILE          
SEQRES   3 B   64  ALA ARG LEU GLU GLU LYS VAL LYS THR LEU LYS ALA GLN          
SEQRES   4 B   64  ASN SER GLU LEU ALA SER THR ALA ASN MET LEU ARG GLU          
SEQRES   5 B   64  GLN VAL ALA GLN LEU LYS GLN LYS VAL MET ASN HIS              
HET    5CM  D  29      20                                                       
HETNAM     5CM 5-METHYL-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE                      
FORMUL   2  5CM    C10 H16 N3 O7 P                                              
FORMUL   5  HOH   *120(H2 O)                                                    
HELIX    1 AA1 LYS A  254  LYS A  311  1                                  58    
HELIX    2 AA2 LYS B  254  MET B  313  1                                  60    
LINK         O3'  DA D  28                 P   5CM D  29     1555   1555  1.61  
LINK         O3' 5CM D  29                 P    DG D  30     1555   1555  1.62  
CRYST1  158.871   42.490   45.167  90.00  98.01  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.006294  0.000000  0.000886        0.00000                         
SCALE2      0.000000  0.023535  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.022358        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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