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Database: PDB
Entry: 5TY4
LinkDB: 5TY4
Original site: 5TY4 
HEADER    TRANSFERASE                             18-NOV-16   5TY4              
TITLE     MICROED STRUCTURE OF A COMPLEX BETWEEN MONOMERIC TGF-B AND ITS        
TITLE    2 RECEPTOR, TBRII, AT 2.9 A RESOLUTION                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TGF-BETA RECEPTOR TYPE-2;                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: TGFR-2, TGF-BETA TYPE II RECEPTOR, TRANSFORMING GROWTH      
COMPND   5 FACTOR-BETA RECEPTOR TYPE II, TBETAR-II;                             
COMPND   6 EC: 2.7.11.30;                                                       
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: MMTGF-B2-7M;                                               
COMPND  10 CHAIN: B;                                                            
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TGFBR2;                                                        
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  13 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    TRANSFERASE                                                           
EXPDTA    ELECTRON CRYSTALLOGRAPHY                                              
AUTHOR    S.C.WEISS,M.J.DE LA CRUZ,J.HATTNE,D.SHI,F.E.REYES,G.CALLERO,T.GONEN   
REVDAT   4   22-AUG-18 5TY4    1       REMARK                                   
REVDAT   3   18-JUL-18 5TY4    1       REMARK                                   
REVDAT   2   09-AUG-17 5TY4    1       REMARK                                   
REVDAT   1   26-APR-17 5TY4    0                                                
JRNL        AUTH   M.J.DE LA CRUZ,J.HATTNE,D.SHI,P.SEIDLER,J.RODRIGUEZ,         
JRNL        AUTH 2 F.E.REYES,M.R.SAWAYA,D.CASCIO,S.C.WEISS,S.K.KIM,C.S.HINCK,   
JRNL        AUTH 3 A.P.HINCK,G.CALERO,D.EISENBERG,T.GONEN                       
JRNL        TITL   ATOMIC-RESOLUTION STRUCTURES FROM FRAGMENTED PROTEIN         
JRNL        TITL 2 CRYSTALS WITH THE CRYOEM METHOD MICROED.                     
JRNL        REF    NAT. METHODS                  V.  14   399 2017              
JRNL        REFN                   ESSN 1548-7105                               
JRNL        PMID   28192420                                                     
JRNL        DOI    10.1038/NMETH.4178                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.90 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.10                                          
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.90                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 26.64                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 68.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 3848                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.295                           
REMARK   3   R VALUE            (WORKING SET) : 0.292                           
REMARK   3   FREE R VALUE                     : 0.328                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 7.540                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 290                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 26.6400 -  3.6523    0.69     1810   154  0.2639 0.3133        
REMARK   3     2  3.6523 -  2.9001    0.69     1748   136  0.3611 0.3687        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.400            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 30.990           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 64.00                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.012           1366                                  
REMARK   3   ANGLE     :  1.573           1847                                  
REMARK   3   CHIRALITY :  0.067            200                                  
REMARK   3   PLANARITY :  0.009            231                                  
REMARK   3   DIHEDRAL  : 19.181            864                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5TY4 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 29-NOV-16.                  
REMARK 100 THE DEPOSITION ID IS D_1000224688.                                   
REMARK 240                                                                      
REMARK 240 EXPERIMENTAL DETAILS                                                 
REMARK 240   RECONSTRUCTION METHOD          : CRYSTALLOGRAPHY                   
REMARK 240   SAMPLE TYPE                    : 3D ARRAY                          
REMARK 240   SPECIMEN TYPE                  : NULL                              
REMARK 240 DATA ACQUISITION                                                     
REMARK 240   DATE OF DATA COLLECTION        : 04-MAY-16                         
REMARK 240   TEMPERATURE           (KELVIN) : 293.0                             
REMARK 240   PH                             : 7.50                              
REMARK 240   NUMBER OF CRYSTALS USED        : 1                                 
REMARK 240   MICROSCOPE MODEL               : FEI TECNAI F20                    
REMARK 240   DETECTOR TYPE                  : TVIPS TEMCAM-F416 (4K X 4K)       
REMARK 240   ACCELERATION VOLTAGE (KV)      : 200                               
REMARK 240   NUMBER OF UNIQUE REFLECTIONS   : 3884                              
REMARK 240   RESOLUTION RANGE HIGH      (A) : 2.900                             
REMARK 240   RESOLUTION RANGE LOW       (A) : 26.640                            
REMARK 240   DATA SCALING SOFTWARE          : AIMLESS 0.5.27                    
REMARK 240   COMPLETENESS FOR RANGE     (%) : 71.9                              
REMARK 240   DATA REDUNDANCY                : 3.800                             
REMARK 240 IN THE HIGHEST RESOLUTION SHELL                                      
REMARK 240   HIGHEST RESOLUTION SHELL, RANGE HIGH (A) :2.90                     
REMARK 240   HIGHEST RESOLUTION SHELL, RANGE LOW  (A) :3.07                     
REMARK 240   COMPLETENESS FOR SHELL     (%) : 71.3                              
REMARK 240   DATA REDUNDANCY IN SHELL       : 3.90                              
REMARK 240   R MERGE FOR SHELL          (I) : 2.02400                           
REMARK 240   METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT      
REMARK 240   SOFTWARE USED                  : PHASER 2.6.0                      
REMARK 240   STARTING MODEL                 : PDB ENTRY 1KTZ                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       20.76500            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       39.75400            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       35.66500            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       39.75400            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       20.76500            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       35.66500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 860 ANGSTROM**2                           
REMARK 350 SURFACE AREA OF THE COMPLEX: 10010 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -2.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ASP A    92                                                      
REMARK 465     GLU A    93                                                      
REMARK 465     ALA B   345                                                      
REMARK 465     GLY B   346                                                      
REMARK 465     ALA B   347                                                      
REMARK 465     CYS B   348                                                      
REMARK 465     PRO B   349                                                      
REMARK 465     TYR B   350                                                      
REMARK 465     LEU B   351                                                      
REMARK 465     TRP B   352                                                      
REMARK 465     SER B   353                                                      
REMARK 465     SER B   354                                                      
REMARK 465     ASP B   355                                                      
REMARK 465     THR B   356                                                      
REMARK 465     GLN B   357                                                      
REMARK 465     HIS B   358                                                      
REMARK 465     SER B   359                                                      
REMARK 465     ARG B   360                                                      
REMARK 465     VAL B   361                                                      
REMARK 465     LEU B   362                                                      
REMARK 465     SER B   363                                                      
REMARK 465     LEU B   364                                                      
REMARK 465     TYR B   365                                                      
REMARK 465     ASN B   366                                                      
REMARK 465     THR B   367                                                      
REMARK 465     ILE B   368                                                      
REMARK 465     ASN B   369                                                      
REMARK 465     PRO B   370                                                      
REMARK 465     GLU B   371                                                      
REMARK 465     ALA B   372                                                      
REMARK 465     SER B   373                                                      
REMARK 465     ALA B   374                                                      
REMARK 465     SER B   375                                                      
REMARK 465     PRO B   376                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     CYS B 377    SG                                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   SG   CYS A    84     SG   CYS A   143              1.09            
REMARK 500   NE2  GLN A    49     O    SER A   139              2.16            
REMARK 500   O    LEU A    50     N    GLU A   142              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    CYS A  51   CB    CYS A  51   SG      0.130                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    CYS A  84   CA  -  CB  -  SG  ANGL. DEV. =   7.8 DEGREES          
REMARK 500    CYS B 409   CA  -  CB  -  SG  ANGL. DEV. =   7.2 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASP A  55     -152.26     61.21                                   
REMARK 500    ASP A  62     -113.88   -148.27                                   
REMARK 500    ASP A 115       35.31    -92.31                                   
REMARK 500    CYS A 143      -73.40    -20.18                                   
REMARK 500    ASN A 144       30.65    -79.52                                   
REMARK 500    PHE B 324      -55.97    -22.81                                   
REMARK 500    ASP B 327      -65.99   -125.92                                   
REMARK 500    ASN B 342      157.59     84.68                                   
REMARK 500    GLN B 381      -61.39    -93.02                                   
REMARK 500    LYS B 407      -85.11   -131.54                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 LEU B  317     ARG B  318                 -139.38                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-8216   RELATED DB: EMDB                              
REMARK 900 RELATED ID: EMD-8217   RELATED DB: EMDB                              
REMARK 900 RELATED ID: EMD-8218   RELATED DB: EMDB                              
REMARK 900 RELATED ID: EMD-8219   RELATED DB: EMDB                              
REMARK 900 RELATED ID: EMD-8220   RELATED DB: EMDB                              
REMARK 900 RELATED ID: EMD-8221   RELATED DB: EMDB                              
REMARK 900 RELATED ID: EMD-8222   RELATED DB: EMDB                              
REMARK 900 RELATED ID: 5K7N   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5K7O   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5K7P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5K7Q   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5K7R   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5K7S   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5K7T   RELATED DB: PDB                                   
REMARK 900 RELATED ID: EMD-8472   RELATED DB: EMDB                              
REMARK 900 MICROED STRUCTURE OF A COMPLEX BETWEEN MONOMERIC TGF-B AND ITS       
REMARK 900 RECEPTOR, TBRII, AT 2.9 A RESOLUTION                                 
DBREF  5TY4 A   47   149  UNP    P37173   TGFR2_HUMAN     72    174             
DBREF  5TY4 B  315   411  PDB    5TY4     5TY4           315    411             
SEQADV 5TY4 THR A  120  UNP  P37173    LYS   145 CONFLICT                       
SEQRES   1 A  103  PHE PRO GLN LEU CYS LYS PHE CYS ASP VAL ARG PHE SER          
SEQRES   2 A  103  THR CYS ASP ASN GLN LYS SER CYS MET SER ASN CYS SER          
SEQRES   3 A  103  ILE THR SER ILE CYS GLU LYS PRO GLN GLU VAL CYS VAL          
SEQRES   4 A  103  ALA VAL TRP ARG LYS ASN ASP GLU ASN ILE THR LEU GLU          
SEQRES   5 A  103  THR VAL CYS HIS ASP PRO LYS LEU PRO TYR HIS ASP PHE          
SEQRES   6 A  103  ILE LEU GLU ASP ALA ALA SER PRO THR CYS ILE MET LYS          
SEQRES   7 A  103  GLU LYS LYS LYS PRO GLY GLU THR PHE PHE MET CYS SER          
SEQRES   8 A  103  CYS SER SER ASP GLU CYS ASN ASP ASN ILE ILE PHE              
SEQRES   1 B   97  CYS CYS LEU ARG PRO LEU TYR ILE ASP PHE ARG LYS ASP          
SEQRES   2 B   97  LEU GLY TRP LYS TRP ILE HIS GLU PRO LYS GLY TYR ASN          
SEQRES   3 B   97  ALA ASN PHE CYS ALA GLY ALA CYS PRO TYR LEU TRP SER          
SEQRES   4 B   97  SER ASP THR GLN HIS SER ARG VAL LEU SER LEU TYR ASN          
SEQRES   5 B   97  THR ILE ASN PRO GLU ALA SER ALA SER PRO CYS CYS VAL          
SEQRES   6 B   97  SER GLN ASP LEU GLU PRO LEU THR ILE VAL TYR TYR VAL          
SEQRES   7 B   97  GLY ARG LYS PRO LYS VAL GLU GLN LEU SER ASN MET ILE          
SEQRES   8 B   97  VAL LYS SER CYS LYS CYS                                      
HELIX    1 AA1 GLU A  142  ASN A  144  5                                   3    
SHEET    1 AA1 5 ASP A  55  PHE A  58  0                                        
SHEET    2 AA1 5 THR A  96  HIS A 102 -1  O  LEU A  97   N  ARG A  57           
SHEET    3 AA1 5 VAL A  83  ARG A  89 -1  N  VAL A  83   O  HIS A 102           
SHEET    4 AA1 5 PHE A 134  CYS A 138 -1  O  CYS A 138   N  CYS A  84           
SHEET    5 AA1 5 LYS A 124  GLU A 125 -1  N  LYS A 124   O  MET A 135           
SHEET    1 AA2 2 SER A  66  MET A  68  0                                        
SHEET    2 AA2 2 ASN A 146  ILE A 148 -1  O  ILE A 147   N  CYS A  67           
SHEET    1 AA3 2 VAL B 379  SER B 380  0                                        
SHEET    2 AA3 2 CYS B 409  LYS B 410 -1  O  LYS B 410   N  VAL B 379           
SHEET    1 AA4 2 LEU B 383  TYR B 391  0                                        
SHEET    2 AA4 2 PRO B 396  VAL B 406 -1  O  LEU B 401   N  LEU B 386           
SSBOND   1 CYS A   51    CYS A   84                          1555   1555  2.01  
SSBOND   2 CYS A   51    CYS A  138                          1555   1555  2.07  
SSBOND   3 CYS A   51    CYS A  143                          1555   1555  1.80  
SSBOND   4 CYS A   54    CYS A   71                          1555   1555  2.03  
SSBOND   5 CYS A   61    CYS A   67                          1555   1555  2.04  
SSBOND   6 CYS A   77    CYS A  101                          1555   1555  2.03  
SSBOND   7 CYS A   84    CYS A  138                          1555   1555  2.03  
SSBOND   8 CYS A  121    CYS A  136                          1555   1555  2.03  
SSBOND   9 CYS A  138    CYS A  143                          1555   1555  2.04  
SSBOND  10 CYS B  315    CYS B  378                          1555   1555  2.03  
SSBOND  11 CYS B  344    CYS B  409                          1555   1555  2.07  
CISPEP   1 GLU B  335    PRO B  336          0         2.14                     
CRYST1   41.530   71.330   79.508  90.00  90.00  90.00 P 21 21 21    4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.024079  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.014019  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.012577        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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