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Database: PDB
Entry: 5UZ7
LinkDB: 5UZ7
Original site: 5UZ7 
HEADER    SIGNALING PROTEIN                       24-FEB-17   5UZ7              
TITLE     VOLTA PHASE PLATE CRYO-ELECTRON MICROSCOPY STRUCTURE OF A CALCITONIN  
TITLE    2 RECEPTOR-HETEROTRIMERIC GS PROTEIN COMPLEX                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(S) SUBUNIT ALPHA      
COMPND   3 ISOFORMS SHORT;                                                      
COMPND   4 CHAIN: A;                                                            
COMPND   5 SYNONYM: ADENYLATE CYCLASE-STIMULATING G ALPHA PROTEIN;              
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) SUBUNIT  
COMPND   9 BETA-1;                                                              
COMPND  10 CHAIN: B;                                                            
COMPND  11 SYNONYM: TRANSDUCIN BETA CHAIN 1;                                    
COMPND  12 ENGINEERED: YES;                                                     
COMPND  13 MOL_ID: 3;                                                           
COMPND  14 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(O) SUBUNIT  
COMPND  15 GAMMA-2;                                                             
COMPND  16 CHAIN: G;                                                            
COMPND  17 SYNONYM: G GAMMA-I;                                                  
COMPND  18 ENGINEERED: YES;                                                     
COMPND  19 MOL_ID: 4;                                                           
COMPND  20 MOLECULE: NANOBODY 35;                                               
COMPND  21 CHAIN: N;                                                            
COMPND  22 ENGINEERED: YES;                                                     
COMPND  23 MOL_ID: 5;                                                           
COMPND  24 MOLECULE: CALCITONIN RECEPTOR;                                       
COMPND  25 CHAIN: R;                                                            
COMPND  26 SYNONYM: CT-R;                                                       
COMPND  27 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: GNAS, GNAS1, GSP;                                              
SOURCE   6 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: GNB1;                                                          
SOURCE  13 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  15 MOL_ID: 3;                                                           
SOURCE  16 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  17 ORGANISM_COMMON: HUMAN;                                              
SOURCE  18 ORGANISM_TAXID: 9606;                                                
SOURCE  19 GENE: GNG2;                                                          
SOURCE  20 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  21 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  22 MOL_ID: 4;                                                           
SOURCE  23 ORGANISM_SCIENTIFIC: LAMA GLAMA;                                     
SOURCE  24 ORGANISM_TAXID: 9844;                                                
SOURCE  25 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  26 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  27 MOL_ID: 5;                                                           
SOURCE  28 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  29 ORGANISM_COMMON: HUMAN;                                              
SOURCE  30 ORGANISM_TAXID: 9606;                                                
SOURCE  31 GENE: CALCR;                                                         
SOURCE  32 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  33 EXPRESSION_SYSTEM_TAXID: 7111                                        
KEYWDS    CLASS B G PROTEIN-COUPLED RECEPTOR AGONIST-RECEPTOR-G PROTEIN TERNARY 
KEYWDS   2 COMPLEX CALCITONIN RECEPTOR ACTIVE-STATE G PROTEIN-COUPLED RECEPTOR, 
KEYWDS   3 SIGNALING PROTEIN                                                    
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    Y.L.LIANG,M.KHOSHOUEI,M.RADJAINIA,Y.ZHANG,A.GLUKHOVA,J.TARRASCH,      
AUTHOR   2 D.M.THAL,S.G.B.FURNESS,G.CHRISTOPOULOS,T.COUDRAT,R.DANEV,            
AUTHOR   3 W.BAUMEISTER,L.J.MILLER,A.CHRISTOPOULOS,B.K.KOBILKA,D.WOOTTEN,       
AUTHOR   4 G.SKINIOTIS,P.M.SEXTON                                               
REVDAT   3   18-JUL-18 5UZ7    1       REMARK                                   
REVDAT   2   14-JUN-17 5UZ7    1       JRNL                                     
REVDAT   1   03-MAY-17 5UZ7    0                                                
JRNL        AUTH   Y.L.LIANG,M.KHOSHOUEI,M.RADJAINIA,Y.ZHANG,A.GLUKHOVA,        
JRNL        AUTH 2 J.TARRASCH,D.M.THAL,S.G.B.FURNESS,G.CHRISTOPOULOS,T.COUDRAT, 
JRNL        AUTH 3 R.DANEV,W.BAUMEISTER,L.J.MILLER,A.CHRISTOPOULOS,B.K.KOBILKA, 
JRNL        AUTH 4 D.WOOTTEN,G.SKINIOTIS,P.M.SEXTON                             
JRNL        TITL   PHASE-PLATE CRYO-EM STRUCTURE OF A CLASS B GPCR-G-PROTEIN    
JRNL        TITL 2 COMPLEX.                                                     
JRNL        REF    NATURE                        V. 546   118 2017              
JRNL        REFN                   ESSN 1476-4687                               
JRNL        PMID   28437792                                                     
JRNL        DOI    10.1038/NATURE22327                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    4.10 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : NULL                                      
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : NULL                                
REMARK   3   REFINEMENT SPACE             : NULL                                
REMARK   3   REFINEMENT PROTOCOL          : NULL                                
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : NULL                                             
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 4.100                          
REMARK   3   NUMBER OF PARTICLES               : 106838                         
REMARK   3   CTF CORRECTION METHOD             : PHASE FLIPPING AND AMPLITUDE   
REMARK   3                                       CORRECTION                     
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 5UZ7 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 08-MAR-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000226509.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : NULL                              
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : COMPLEX OF A FULL-LENGTH,         
REMARK 245                                    ACTIVE-STATE CALCITONIN           
REMARK 245                                    RECEPTOR WITH PEPTIDE LIGAND,     
REMARK 245                                    HETEROTRIMERIC GS PROTEIN AND     
REMARK 245                                    NANO BODY 35.                     
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 0.30                              
REMARK 245   SAMPLE SUPPORT DETAILS         : NULL                              
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : NULL                           
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : NULL                           
REMARK 245   MICROSCOPE MODEL                  : FEI TITAN KRIOS                
REMARK 245   DETECTOR TYPE                     : GATAN K2 SUMMIT (4K X 4K)      
REMARK 245   MINIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MAXIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : NULL                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 50.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : NULL                           
REMARK 245   CALIBRATED MAGNIFICATION          : NULL                           
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 300                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, G, N, R                         
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     GLY A     2                                                      
REMARK 465     CYS A     3                                                      
REMARK 465     LEU A     4                                                      
REMARK 465     GLY A     5                                                      
REMARK 465     ASN A     6                                                      
REMARK 465     SER A     7                                                      
REMARK 465     LYS A     8                                                      
REMARK 465     ALA A    62                                                      
REMARK 465     GLY A    63                                                      
REMARK 465     GLU A    64                                                      
REMARK 465     SER A    65                                                      
REMARK 465     GLY A    66                                                      
REMARK 465     LYS A    67                                                      
REMARK 465     SER A    68                                                      
REMARK 465     THR A    69                                                      
REMARK 465     ILE A    70                                                      
REMARK 465     VAL A    71                                                      
REMARK 465     LYS A    72                                                      
REMARK 465     GLN A    73                                                      
REMARK 465     MET A    74                                                      
REMARK 465     ARG A    75                                                      
REMARK 465     ILE A    76                                                      
REMARK 465     LEU A    77                                                      
REMARK 465     HIS A    78                                                      
REMARK 465     VAL A    79                                                      
REMARK 465     ASN A    80                                                      
REMARK 465     GLY A    81                                                      
REMARK 465     PHE A    82                                                      
REMARK 465     ASN A    83                                                      
REMARK 465     GLY A    84                                                      
REMARK 465     ASP A    85                                                      
REMARK 465     SER A    86                                                      
REMARK 465     GLU A    87                                                      
REMARK 465     LYS A    88                                                      
REMARK 465     ALA A    89                                                      
REMARK 465     THR A    90                                                      
REMARK 465     LYS A    91                                                      
REMARK 465     VAL A    92                                                      
REMARK 465     GLN A    93                                                      
REMARK 465     ASP A    94                                                      
REMARK 465     ILE A    95                                                      
REMARK 465     LYS A    96                                                      
REMARK 465     ASN A    97                                                      
REMARK 465     ASN A    98                                                      
REMARK 465     LEU A    99                                                      
REMARK 465     LYS A   100                                                      
REMARK 465     GLU A   101                                                      
REMARK 465     ALA A   102                                                      
REMARK 465     ILE A   103                                                      
REMARK 465     GLU A   104                                                      
REMARK 465     THR A   105                                                      
REMARK 465     ILE A   106                                                      
REMARK 465     VAL A   107                                                      
REMARK 465     ALA A   108                                                      
REMARK 465     ALA A   109                                                      
REMARK 465     MET A   110                                                      
REMARK 465     SER A   111                                                      
REMARK 465     ASN A   112                                                      
REMARK 465     LEU A   113                                                      
REMARK 465     VAL A   114                                                      
REMARK 465     PRO A   115                                                      
REMARK 465     PRO A   116                                                      
REMARK 465     VAL A   117                                                      
REMARK 465     GLU A   118                                                      
REMARK 465     LEU A   119                                                      
REMARK 465     ALA A   120                                                      
REMARK 465     ASN A   121                                                      
REMARK 465     PRO A   122                                                      
REMARK 465     GLU A   123                                                      
REMARK 465     ASN A   124                                                      
REMARK 465     GLN A   125                                                      
REMARK 465     PHE A   126                                                      
REMARK 465     ARG A   127                                                      
REMARK 465     VAL A   128                                                      
REMARK 465     ASP A   129                                                      
REMARK 465     TYR A   130                                                      
REMARK 465     ILE A   131                                                      
REMARK 465     LEU A   132                                                      
REMARK 465     SER A   133                                                      
REMARK 465     VAL A   134                                                      
REMARK 465     MET A   135                                                      
REMARK 465     ASN A   136                                                      
REMARK 465     VAL A   137                                                      
REMARK 465     PRO A   138                                                      
REMARK 465     ASP A   139                                                      
REMARK 465     PHE A   140                                                      
REMARK 465     ASP A   141                                                      
REMARK 465     PHE A   142                                                      
REMARK 465     PRO A   143                                                      
REMARK 465     PRO A   144                                                      
REMARK 465     GLU A   145                                                      
REMARK 465     PHE A   146                                                      
REMARK 465     TYR A   147                                                      
REMARK 465     GLU A   148                                                      
REMARK 465     HIS A   149                                                      
REMARK 465     ALA A   150                                                      
REMARK 465     LYS A   151                                                      
REMARK 465     ALA A   152                                                      
REMARK 465     LEU A   153                                                      
REMARK 465     TRP A   154                                                      
REMARK 465     GLU A   155                                                      
REMARK 465     ASP A   156                                                      
REMARK 465     GLU A   157                                                      
REMARK 465     GLY A   158                                                      
REMARK 465     VAL A   159                                                      
REMARK 465     ARG A   160                                                      
REMARK 465     ALA A   161                                                      
REMARK 465     CYS A   162                                                      
REMARK 465     TYR A   163                                                      
REMARK 465     GLU A   164                                                      
REMARK 465     ARG A   165                                                      
REMARK 465     SER A   166                                                      
REMARK 465     ASN A   167                                                      
REMARK 465     GLU A   168                                                      
REMARK 465     TYR A   169                                                      
REMARK 465     GLN A   170                                                      
REMARK 465     LEU A   171                                                      
REMARK 465     ILE A   172                                                      
REMARK 465     ASP A   173                                                      
REMARK 465     CYS A   174                                                      
REMARK 465     ALA A   175                                                      
REMARK 465     GLN A   176                                                      
REMARK 465     TYR A   177                                                      
REMARK 465     PHE A   178                                                      
REMARK 465     LEU A   179                                                      
REMARK 465     ASP A   180                                                      
REMARK 465     LYS A   181                                                      
REMARK 465     ILE A   182                                                      
REMARK 465     ASP A   183                                                      
REMARK 465     VAL A   184                                                      
REMARK 465     ILE A   185                                                      
REMARK 465     LYS A   186                                                      
REMARK 465     GLN A   187                                                      
REMARK 465     ALA A   188                                                      
REMARK 465     ASP A   189                                                      
REMARK 465     TYR A   190                                                      
REMARK 465     VAL A   191                                                      
REMARK 465     PRO A   192                                                      
REMARK 465     SER A   193                                                      
REMARK 465     ASP A   194                                                      
REMARK 465     GLN A   195                                                      
REMARK 465     ASP A   196                                                      
REMARK 465     LEU A   197                                                      
REMARK 465     LEU A   198                                                      
REMARK 465     ARG A   199                                                      
REMARK 465     CYS A   200                                                      
REMARK 465     ARG A   201                                                      
REMARK 465     VAL A   202                                                      
REMARK 465     LEU A   203                                                      
REMARK 465     THR A   204                                                      
REMARK 465     SER A   205                                                      
REMARK 465     GLY A   206                                                      
REMARK 465     SER A   251                                                      
REMARK 465     SER A   252                                                      
REMARK 465     TYR A   253                                                      
REMARK 465     ASN A   254                                                      
REMARK 465     MET A   255                                                      
REMARK 465     VAL A   256                                                      
REMARK 465     ILE A   257                                                      
REMARK 465     ARG A   258                                                      
REMARK 465     GLU A   259                                                      
REMARK 465     ASP A   260                                                      
REMARK 465     ASN A   261                                                      
REMARK 465     GLN A   262                                                      
REMARK 465     THR A   263                                                      
REMARK 465     LYS A   293                                                      
REMARK 465     GLN A   294                                                      
REMARK 465     ASP A   295                                                      
REMARK 465     LEU A   296                                                      
REMARK 465     LEU A   297                                                      
REMARK 465     ALA A   298                                                      
REMARK 465     GLU A   299                                                      
REMARK 465     LYS A   300                                                      
REMARK 465     VAL A   301                                                      
REMARK 465     LEU A   302                                                      
REMARK 465     ALA A   303                                                      
REMARK 465     GLY A   304                                                      
REMARK 465     LYS A   305                                                      
REMARK 465     SER A   306                                                      
REMARK 465     LYS A   307                                                      
REMARK 465     ILE A   308                                                      
REMARK 465     GLU A   322                                                      
REMARK 465     ASP A   323                                                      
REMARK 465     ALA A   324                                                      
REMARK 465     THR A   325                                                      
REMARK 465     PRO A   326                                                      
REMARK 465     GLU A   327                                                      
REMARK 465     PRO A   328                                                      
REMARK 465     GLY A   329                                                      
REMARK 465     GLU A   330                                                      
REMARK 465     ALA A   366                                                      
REMARK 465     VAL A   367                                                      
REMARK 465     ASP A   368                                                      
REMARK 465     THR A   369                                                      
REMARK 465     MET B   -10                                                      
REMARK 465     HIS B    -9                                                      
REMARK 465     HIS B    -8                                                      
REMARK 465     HIS B    -7                                                      
REMARK 465     HIS B    -6                                                      
REMARK 465     HIS B    -5                                                      
REMARK 465     HIS B    -4                                                      
REMARK 465     GLY B    -3                                                      
REMARK 465     SER B    -2                                                      
REMARK 465     LEU B    -1                                                      
REMARK 465     LEU B     0                                                      
REMARK 465     MET G     1                                                      
REMARK 465     ALA G     2                                                      
REMARK 465     SER G     3                                                      
REMARK 465     ASN G     4                                                      
REMARK 465     ASN G     5                                                      
REMARK 465     THR G     6                                                      
REMARK 465     ALA G     7                                                      
REMARK 465     GLU G    63                                                      
REMARK 465     LYS G    64                                                      
REMARK 465     LYS G    65                                                      
REMARK 465     PHE G    66                                                      
REMARK 465     PHE G    67                                                      
REMARK 465     CYS G    68                                                      
REMARK 465     HIS N   129                                                      
REMARK 465     HIS N   130                                                      
REMARK 465     HIS N   131                                                      
REMARK 465     HIS N   132                                                      
REMARK 465     HIS N   133                                                      
REMARK 465     HIS N   134                                                      
REMARK 465     GLU N   135                                                      
REMARK 465     PRO N   136                                                      
REMARK 465     GLU N   137                                                      
REMARK 465     ALA N   138                                                      
REMARK 465     MET R    -7                                                      
REMARK 465     LYS R    -6                                                      
REMARK 465     THR R    -5                                                      
REMARK 465     ILE R    -4                                                      
REMARK 465     ILE R    -3                                                      
REMARK 465     ALA R    -2                                                      
REMARK 465     LEU R    -1                                                      
REMARK 465     SER R     0                                                      
REMARK 465     TYR R     1                                                      
REMARK 465     ILE R     2                                                      
REMARK 465     PHE R     3                                                      
REMARK 465     CYS R     4                                                      
REMARK 465     LEU R     5                                                      
REMARK 465     VAL R     6                                                      
REMARK 465     PHE R     7                                                      
REMARK 465     ALA R     8                                                      
REMARK 465     ASP R     9                                                      
REMARK 465     TYR R    10                                                      
REMARK 465     LYS R    11                                                      
REMARK 465     ASP R    12                                                      
REMARK 465     ASP R    13                                                      
REMARK 465     ASP R    14                                                      
REMARK 465     ASP R    15                                                      
REMARK 465     LEU R    16                                                      
REMARK 465     GLU R    17                                                      
REMARK 465     VAL R    18                                                      
REMARK 465     LEU R    19                                                      
REMARK 465     PHE R    20                                                      
REMARK 465     GLN R    21                                                      
REMARK 465     GLY R    22                                                      
REMARK 465     PRO R    23                                                      
REMARK 465     ALA R    24                                                      
REMARK 465     ALA R    25                                                      
REMARK 465     PHE R    26                                                      
REMARK 465     SER R    27                                                      
REMARK 465     ASN R    28                                                      
REMARK 465     GLN R    29                                                      
REMARK 465     THR R    30                                                      
REMARK 465     TYR R    31                                                      
REMARK 465     PRO R    32                                                      
REMARK 465     THR R    33                                                      
REMARK 465     ILE R    34                                                      
REMARK 465     GLU R    35                                                      
REMARK 465     PRO R    36                                                      
REMARK 465     LYS R    37                                                      
REMARK 465     PRO R    38                                                      
REMARK 465     PHE R    39                                                      
REMARK 465     LEU R    40                                                      
REMARK 465     TYR R    41                                                      
REMARK 465     VAL R    42                                                      
REMARK 465     VAL R    43                                                      
REMARK 465     GLY R    44                                                      
REMARK 465     ARG R    45                                                      
REMARK 465     LYS R    46                                                      
REMARK 465     LYS R    47                                                      
REMARK 465     MET R    48                                                      
REMARK 465     MET R    49                                                      
REMARK 465     ASP R    50                                                      
REMARK 465     ALA R    51                                                      
REMARK 465     GLN R    52                                                      
REMARK 465     TYR R    53                                                      
REMARK 465     LYS R    54                                                      
REMARK 465     CYS R    55                                                      
REMARK 465     TYR R    56                                                      
REMARK 465     ASP R    57                                                      
REMARK 465     ARG R    58                                                      
REMARK 465     MET R    59                                                      
REMARK 465     GLN R    60                                                      
REMARK 465     GLN R    61                                                      
REMARK 465     LEU R    62                                                      
REMARK 465     PRO R    63                                                      
REMARK 465     ALA R    64                                                      
REMARK 465     TYR R    65                                                      
REMARK 465     GLN R    66                                                      
REMARK 465     GLY R    67                                                      
REMARK 465     GLU R    68                                                      
REMARK 465     GLY R    69                                                      
REMARK 465     PRO R    70                                                      
REMARK 465     TYR R    71                                                      
REMARK 465     CYS R    72                                                      
REMARK 465     ASN R    73                                                      
REMARK 465     ARG R    74                                                      
REMARK 465     THR R    75                                                      
REMARK 465     TRP R    76                                                      
REMARK 465     ASP R    77                                                      
REMARK 465     GLY R    78                                                      
REMARK 465     TRP R    79                                                      
REMARK 465     LEU R    80                                                      
REMARK 465     CYS R    81                                                      
REMARK 465     TRP R    82                                                      
REMARK 465     ASP R    83                                                      
REMARK 465     ASP R    84                                                      
REMARK 465     THR R    85                                                      
REMARK 465     PRO R    86                                                      
REMARK 465     ALA R    87                                                      
REMARK 465     GLY R    88                                                      
REMARK 465     VAL R    89                                                      
REMARK 465     LEU R    90                                                      
REMARK 465     SER R    91                                                      
REMARK 465     TYR R    92                                                      
REMARK 465     GLN R    93                                                      
REMARK 465     PHE R    94                                                      
REMARK 465     CYS R    95                                                      
REMARK 465     PRO R    96                                                      
REMARK 465     ASP R    97                                                      
REMARK 465     TYR R    98                                                      
REMARK 465     PHE R    99                                                      
REMARK 465     PRO R   100                                                      
REMARK 465     ASP R   101                                                      
REMARK 465     PHE R   102                                                      
REMARK 465     ASP R   103                                                      
REMARK 465     PRO R   104                                                      
REMARK 465     SER R   105                                                      
REMARK 465     GLU R   106                                                      
REMARK 465     LYS R   107                                                      
REMARK 465     VAL R   108                                                      
REMARK 465     THR R   109                                                      
REMARK 465     LYS R   110                                                      
REMARK 465     TYR R   111                                                      
REMARK 465     CYS R   112                                                      
REMARK 465     ASP R   113                                                      
REMARK 465     GLU R   114                                                      
REMARK 465     LYS R   115                                                      
REMARK 465     GLY R   116                                                      
REMARK 465     VAL R   117                                                      
REMARK 465     TRP R   118                                                      
REMARK 465     PHE R   119                                                      
REMARK 465     LYS R   120                                                      
REMARK 465     HIS R   121                                                      
REMARK 465     PRO R   122                                                      
REMARK 465     GLU R   123                                                      
REMARK 465     ASN R   124                                                      
REMARK 465     ASN R   125                                                      
REMARK 465     ARG R   126                                                      
REMARK 465     THR R   127                                                      
REMARK 465     TRP R   128                                                      
REMARK 465     SER R   129                                                      
REMARK 465     ASN R   130                                                      
REMARK 465     TYR R   131                                                      
REMARK 465     THR R   132                                                      
REMARK 465     MET R   133                                                      
REMARK 465     CYS R   134                                                      
REMARK 465     ASN R   135                                                      
REMARK 465     VAL R   206                                                      
REMARK 465     PRO R   207                                                      
REMARK 465     ASN R   208                                                      
REMARK 465     GLY R   209                                                      
REMARK 465     GLU R   210                                                      
REMARK 465     LEU R   211                                                      
REMARK 465     VAL R   212                                                      
REMARK 465     GLU R   332                                                      
REMARK 465     ALA R   333                                                      
REMARK 465     GLU R   334                                                      
REMARK 465     SER R   335                                                      
REMARK 465     HIS R   336                                                      
REMARK 465     TRP R   361                                                      
REMARK 465     ARG R   362                                                      
REMARK 465     PRO R   363                                                      
REMARK 465     SER R   364                                                      
REMARK 465     ASN R   365                                                      
REMARK 465     ARG R   419                                                      
REMARK 465     ARG R   420                                                      
REMARK 465     PRO R   421                                                      
REMARK 465     SER R   422                                                      
REMARK 465     ASN R   423                                                      
REMARK 465     ARG R   424                                                      
REMARK 465     SER R   425                                                      
REMARK 465     ALA R   426                                                      
REMARK 465     ARG R   427                                                      
REMARK 465     ALA R   428                                                      
REMARK 465     ALA R   429                                                      
REMARK 465     ALA R   430                                                      
REMARK 465     ALA R   431                                                      
REMARK 465     ALA R   432                                                      
REMARK 465     ALA R   433                                                      
REMARK 465     GLU R   434                                                      
REMARK 465     ALA R   435                                                      
REMARK 465     GLY R   436                                                      
REMARK 465     ASP R   437                                                      
REMARK 465     ILE R   438                                                      
REMARK 465     PRO R   439                                                      
REMARK 465     ILE R   440                                                      
REMARK 465     TYR R   441                                                      
REMARK 465     ILE R   442                                                      
REMARK 465     CYS R   443                                                      
REMARK 465     HIS R   444                                                      
REMARK 465     GLN R   445                                                      
REMARK 465     GLU R   446                                                      
REMARK 465     LEU R   447                                                      
REMARK 465     ARG R   448                                                      
REMARK 465     ASN R   449                                                      
REMARK 465     GLU R   450                                                      
REMARK 465     PRO R   451                                                      
REMARK 465     ALA R   452                                                      
REMARK 465     ASN R   453                                                      
REMARK 465     ASN R   454                                                      
REMARK 465     GLN R   455                                                      
REMARK 465     GLY R   456                                                      
REMARK 465     GLU R   457                                                      
REMARK 465     GLU R   458                                                      
REMARK 465     SER R   459                                                      
REMARK 465     ALA R   460                                                      
REMARK 465     GLU R   461                                                      
REMARK 465     ILE R   462                                                      
REMARK 465     ILE R   463                                                      
REMARK 465     PRO R   464                                                      
REMARK 465     LEU R   465                                                      
REMARK 465     ASN R   466                                                      
REMARK 465     ILE R   467                                                      
REMARK 465     ILE R   468                                                      
REMARK 465     GLU R   469                                                      
REMARK 465     GLN R   470                                                      
REMARK 465     GLU R   471                                                      
REMARK 465     SER R   472                                                      
REMARK 465     SER R   473                                                      
REMARK 465     ALA R   474                                                      
REMARK 465     PRO R   475                                                      
REMARK 465     ALA R   476                                                      
REMARK 465     GLY R   477                                                      
REMARK 465     LEU R   478                                                      
REMARK 465     GLU R   479                                                      
REMARK 465     VAL R   480                                                      
REMARK 465     LEU R   481                                                      
REMARK 465     PHE R   482                                                      
REMARK 465     GLN R   483                                                      
REMARK 465     GLY R   484                                                      
REMARK 465     PRO R   485                                                      
REMARK 465     HIS R   486                                                      
REMARK 465     HIS R   487                                                      
REMARK 465     HIS R   488                                                      
REMARK 465     HIS R   489                                                      
REMARK 465     HIS R   490                                                      
REMARK 465     HIS R   491                                                      
REMARK 465     HIS R   492                                                      
REMARK 465     HIS R   493                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A 216    CG   CD   CE   NZ                                   
REMARK 470     ASP A 240    CG   OD1  OD2                                       
REMARK 470     ARG A 283    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG A 333    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP A 354    CG   OD1  OD2                                       
REMARK 470     GLN B   1    CB   CG   CD   OE1  NE2                             
REMARK 470     ARG B  42    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 129    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 130    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 172    CG   CD   OE1  OE2                                  
REMARK 470     GLN B 175    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 215    CG   CD   OE1  OE2                                  
REMARK 470     MET B 217    CG   SD   CE                                        
REMARK 470     LYS G  32    CG   CD   CE   NZ                                   
REMARK 470     SER N 112    OG                                                  
REMARK 470     GLU R 140    CG   CD   OE1  OE2                                  
REMARK 470     TYR R 150    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     PHE R 172    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ARG R 174    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER R 175    OG                                                  
REMARK 470     LEU R 176    CG   CD1  CD2                                       
REMARK 470     ARG R 213    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG R 214    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP R 215    CG   OD1  OD2                                       
REMARK 470     GLU R 255    CG   CD   OE1  OE2                                  
REMARK 470     LYS R 256    CG   CD   CE   NZ                                   
REMARK 470     GLN R 257    CG   CD   OE1  NE2                                  
REMARK 470     ARG R 258    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LEU R 259    CG   CD1  CD2                                       
REMARK 470     ARG R 260    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     PHE R 285    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ASN R 286    CG   OD1  ND2                                       
REMARK 470     ASP R 287    CG   OD1  OD2                                       
REMARK 470     ASN R 288    CG   OD1  ND2                                       
REMARK 470     TRP R 290    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP R 290    CZ3  CH2                                            
REMARK 470     LEU R 291    CG   CD1  CD2                                       
REMARK 470     SER R 292    OG                                                  
REMARK 470     VAL R 293    CG1  CG2                                            
REMARK 470     GLU R 294    CG   CD   OE1  OE2                                  
REMARK 470     THR R 295    OG1  CG2                                            
REMARK 470     HIS R 296    CG   ND1  CD2  CE1  NE2                             
REMARK 470     THR R 330    OG1  CG2                                            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU B  79   CA  -  CB  -  CG  ANGL. DEV. =  16.6 DEGREES          
REMARK 500    LEU R 142   CA  -  CB  -  CG  ANGL. DEV. =  16.3 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ALA B  26       38.05    -93.58                                   
REMARK 500    THR B  87       39.10     37.63                                   
REMARK 500    ASN B 132     -165.22    -79.35                                   
REMARK 500    ALA B 203     -168.66   -160.60                                   
REMARK 500    ARG B 304       98.69    -68.70                                   
REMARK 500    ASN G  24       58.81    -94.22                                   
REMARK 500    THR N 114       22.75     46.85                                   
REMARK 500    ALA R 251       51.02   -118.81                                   
REMARK 500    TYR R 338       67.01     60.20                                   
REMARK 500    PRO R 350        1.31    -58.85                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-8623   RELATED DB: EMDB                              
REMARK 900 VOLTA PHASE PLATE CRYO-ELECTRON MICROSCOPY STRUCTURE OF A            
REMARK 900 CALCITONIN RECEPTOR-HETEROTRIMERIC GS PROTEIN COMPLEX                
DBREF  5UZ7 A    1   394  UNP    P63092   GNAS2_HUMAN      1    380             
DBREF  5UZ7 B    2   340  UNP    P62873   GBB1_HUMAN       2    340             
DBREF  5UZ7 G    1    68  UNP    P59768   GBG2_HUMAN       1     68             
DBREF  5UZ7 N    1   138  PDB    5UZ7     5UZ7             1    138             
DBREF  5UZ7 R   25   474  UNP    P30988   CALCR_HUMAN     25    474             
SEQADV 5UZ7 MET B  -10  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 HIS B   -9  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 HIS B   -8  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 HIS B   -7  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 HIS B   -6  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 HIS B   -5  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 HIS B   -4  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 GLY B   -3  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 SER B   -2  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 LEU B   -1  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 LEU B    0  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 GLN B    1  UNP  P62873              EXPRESSION TAG                 
SEQADV 5UZ7 MET R   -7  UNP  P30988              INITIATING METHIONINE          
SEQADV 5UZ7 LYS R   -6  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 THR R   -5  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ILE R   -4  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ILE R   -3  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ALA R   -2  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R   -1  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 SER R    0  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 TYR R    1  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ILE R    2  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 PHE R    3  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 CYS R    4  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R    5  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 VAL R    6  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 PHE R    7  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ALA R    8  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ASP R    9  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 TYR R   10  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LYS R   11  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ASP R   12  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ASP R   13  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ASP R   14  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ASP R   15  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R   16  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLU R   17  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 VAL R   18  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R   19  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 PHE R   20  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLN R   21  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLY R   22  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 PRO R   23  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ALA R   24  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R  447  UNP  P30988    PRO   447 CONFLICT                       
SEQADV 5UZ7 PRO R  475  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 ALA R  476  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLY R  477  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R  478  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLU R  479  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 VAL R  480  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 LEU R  481  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 PHE R  482  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLN R  483  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 GLY R  484  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 PRO R  485  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  486  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  487  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  488  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  489  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  490  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  491  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  492  UNP  P30988              EXPRESSION TAG                 
SEQADV 5UZ7 HIS R  493  UNP  P30988              EXPRESSION TAG                 
SEQRES   1 A  380  MET GLY CYS LEU GLY ASN SER LYS THR GLU ASP GLN ARG          
SEQRES   2 A  380  ASN GLU GLU LYS ALA GLN ARG GLU ALA ASN LYS LYS ILE          
SEQRES   3 A  380  GLU LYS GLN LEU GLN LYS ASP LYS GLN VAL TYR ARG ALA          
SEQRES   4 A  380  THR HIS ARG LEU LEU LEU LEU GLY ALA GLY GLU SER GLY          
SEQRES   5 A  380  LYS SER THR ILE VAL LYS GLN MET ARG ILE LEU HIS VAL          
SEQRES   6 A  380  ASN GLY PHE ASN GLY ASP SER GLU LYS ALA THR LYS VAL          
SEQRES   7 A  380  GLN ASP ILE LYS ASN ASN LEU LYS GLU ALA ILE GLU THR          
SEQRES   8 A  380  ILE VAL ALA ALA MET SER ASN LEU VAL PRO PRO VAL GLU          
SEQRES   9 A  380  LEU ALA ASN PRO GLU ASN GLN PHE ARG VAL ASP TYR ILE          
SEQRES  10 A  380  LEU SER VAL MET ASN VAL PRO ASP PHE ASP PHE PRO PRO          
SEQRES  11 A  380  GLU PHE TYR GLU HIS ALA LYS ALA LEU TRP GLU ASP GLU          
SEQRES  12 A  380  GLY VAL ARG ALA CYS TYR GLU ARG SER ASN GLU TYR GLN          
SEQRES  13 A  380  LEU ILE ASP CYS ALA GLN TYR PHE LEU ASP LYS ILE ASP          
SEQRES  14 A  380  VAL ILE LYS GLN ALA ASP TYR VAL PRO SER ASP GLN ASP          
SEQRES  15 A  380  LEU LEU ARG CYS ARG VAL LEU THR SER GLY ILE PHE GLU          
SEQRES  16 A  380  THR LYS PHE GLN VAL ASP LYS VAL ASN PHE HIS MET PHE          
SEQRES  17 A  380  ASP VAL GLY GLY GLN ARG ASP GLU ARG ARG LYS TRP ILE          
SEQRES  18 A  380  GLN CYS PHE ASN ASP VAL THR ALA ILE ILE PHE VAL VAL          
SEQRES  19 A  380  ALA SER SER SER TYR ASN MET VAL ILE ARG GLU ASP ASN          
SEQRES  20 A  380  GLN THR ASN ARG LEU GLN GLU ALA LEU ASN LEU PHE LYS          
SEQRES  21 A  380  SER ILE TRP ASN ASN ARG TRP LEU ARG THR ILE SER VAL          
SEQRES  22 A  380  ILE LEU PHE LEU ASN LYS GLN ASP LEU LEU ALA GLU LYS          
SEQRES  23 A  380  VAL LEU ALA GLY LYS SER LYS ILE GLU ASP TYR PHE PRO          
SEQRES  24 A  380  GLU PHE ALA ARG TYR THR THR PRO GLU ASP ALA THR PRO          
SEQRES  25 A  380  GLU PRO GLY GLU ASP PRO ARG VAL THR ARG ALA LYS TYR          
SEQRES  26 A  380  PHE ILE ARG ASP GLU PHE LEU ARG ILE SER THR ALA SER          
SEQRES  27 A  380  GLY ASP GLY ARG HIS TYR CYS TYR PRO HIS PHE THR CYS          
SEQRES  28 A  380  ALA VAL ASP THR GLU ASN ILE ARG ARG VAL PHE ASN ASP          
SEQRES  29 A  380  CYS ARG ASP ILE ILE GLN ARG MET HIS LEU ARG GLN TYR          
SEQRES  30 A  380  GLU LEU LEU                                                  
SEQRES   1 B  351  MET HIS HIS HIS HIS HIS HIS GLY SER LEU LEU GLN SER          
SEQRES   2 B  351  GLU LEU ASP GLN LEU ARG GLN GLU ALA GLU GLN LEU LYS          
SEQRES   3 B  351  ASN GLN ILE ARG ASP ALA ARG LYS ALA CYS ALA ASP ALA          
SEQRES   4 B  351  THR LEU SER GLN ILE THR ASN ASN ILE ASP PRO VAL GLY          
SEQRES   5 B  351  ARG ILE GLN MET ARG THR ARG ARG THR LEU ARG GLY HIS          
SEQRES   6 B  351  LEU ALA LYS ILE TYR ALA MET HIS TRP GLY THR ASP SER          
SEQRES   7 B  351  ARG LEU LEU VAL SER ALA SER GLN ASP GLY LYS LEU ILE          
SEQRES   8 B  351  ILE TRP ASP SER TYR THR THR ASN LYS VAL HIS ALA ILE          
SEQRES   9 B  351  PRO LEU ARG SER SER TRP VAL MET THR CYS ALA TYR ALA          
SEQRES  10 B  351  PRO SER GLY ASN TYR VAL ALA CYS GLY GLY LEU ASP ASN          
SEQRES  11 B  351  ILE CYS SER ILE TYR ASN LEU LYS THR ARG GLU GLY ASN          
SEQRES  12 B  351  VAL ARG VAL SER ARG GLU LEU ALA GLY HIS THR GLY TYR          
SEQRES  13 B  351  LEU SER CYS CYS ARG PHE LEU ASP ASP ASN GLN ILE VAL          
SEQRES  14 B  351  THR SER SER GLY ASP THR THR CYS ALA LEU TRP ASP ILE          
SEQRES  15 B  351  GLU THR GLY GLN GLN THR THR THR PHE THR GLY HIS THR          
SEQRES  16 B  351  GLY ASP VAL MET SER LEU SER LEU ALA PRO ASP THR ARG          
SEQRES  17 B  351  LEU PHE VAL SER GLY ALA CYS ASP ALA SER ALA LYS LEU          
SEQRES  18 B  351  TRP ASP VAL ARG GLU GLY MET CYS ARG GLN THR PHE THR          
SEQRES  19 B  351  GLY HIS GLU SER ASP ILE ASN ALA ILE CYS PHE PHE PRO          
SEQRES  20 B  351  ASN GLY ASN ALA PHE ALA THR GLY SER ASP ASP ALA THR          
SEQRES  21 B  351  CYS ARG LEU PHE ASP LEU ARG ALA ASP GLN GLU LEU MET          
SEQRES  22 B  351  THR TYR SER HIS ASP ASN ILE ILE CYS GLY ILE THR SER          
SEQRES  23 B  351  VAL SER PHE SER LYS SER GLY ARG LEU LEU LEU ALA GLY          
SEQRES  24 B  351  TYR ASP ASP PHE ASN CYS ASN VAL TRP ASP ALA LEU LYS          
SEQRES  25 B  351  ALA ASP ARG ALA GLY VAL LEU ALA GLY HIS ASP ASN ARG          
SEQRES  26 B  351  VAL SER CYS LEU GLY VAL THR ASP ASP GLY MET ALA VAL          
SEQRES  27 B  351  ALA THR GLY SER TRP ASP SER PHE LEU LYS ILE TRP ASN          
SEQRES   1 G   68  MET ALA SER ASN ASN THR ALA SER ILE ALA GLN ALA ARG          
SEQRES   2 G   68  LYS LEU VAL GLU GLN LEU LYS MET GLU ALA ASN ILE ASP          
SEQRES   3 G   68  ARG ILE LYS VAL SER LYS ALA ALA ALA ASP LEU MET ALA          
SEQRES   4 G   68  TYR CYS GLU ALA HIS ALA LYS GLU ASP PRO LEU LEU THR          
SEQRES   5 G   68  PRO VAL PRO ALA SER GLU ASN PRO PHE ARG GLU LYS LYS          
SEQRES   6 G   68  PHE PHE CYS                                                  
SEQRES   1 N  138  GLN VAL GLN LEU GLN GLU SER GLY GLY GLY LEU VAL GLN          
SEQRES   2 N  138  PRO GLY GLY SER LEU ARG LEU SER CYS ALA ALA SER GLY          
SEQRES   3 N  138  PHE THR PHE SER ASN TYR LYS MET ASN TRP VAL ARG GLN          
SEQRES   4 N  138  ALA PRO GLY LYS GLY LEU GLU TRP VAL SER ASP ILE SER          
SEQRES   5 N  138  GLN SER GLY ALA SER ILE SER TYR THR GLY SER VAL LYS          
SEQRES   6 N  138  GLY ARG PHE THR ILE SER ARG ASP ASN ALA LYS ASN THR          
SEQRES   7 N  138  LEU TYR LEU GLN MET ASN SER LEU LYS PRO GLU ASP THR          
SEQRES   8 N  138  ALA VAL TYR TYR CYS ALA ARG CYS PRO ALA PRO PHE THR          
SEQRES   9 N  138  ARG ASP CYS PHE ASP VAL THR SER THR THR TYR ALA TYR          
SEQRES  10 N  138  ARG GLY GLN GLY THR GLN VAL THR VAL SER SER HIS HIS          
SEQRES  11 N  138  HIS HIS HIS HIS GLU PRO GLU ALA                              
SEQRES   1 R  501  MET LYS THR ILE ILE ALA LEU SER TYR ILE PHE CYS LEU          
SEQRES   2 R  501  VAL PHE ALA ASP TYR LYS ASP ASP ASP ASP LEU GLU VAL          
SEQRES   3 R  501  LEU PHE GLN GLY PRO ALA ALA PHE SER ASN GLN THR TYR          
SEQRES   4 R  501  PRO THR ILE GLU PRO LYS PRO PHE LEU TYR VAL VAL GLY          
SEQRES   5 R  501  ARG LYS LYS MET MET ASP ALA GLN TYR LYS CYS TYR ASP          
SEQRES   6 R  501  ARG MET GLN GLN LEU PRO ALA TYR GLN GLY GLU GLY PRO          
SEQRES   7 R  501  TYR CYS ASN ARG THR TRP ASP GLY TRP LEU CYS TRP ASP          
SEQRES   8 R  501  ASP THR PRO ALA GLY VAL LEU SER TYR GLN PHE CYS PRO          
SEQRES   9 R  501  ASP TYR PHE PRO ASP PHE ASP PRO SER GLU LYS VAL THR          
SEQRES  10 R  501  LYS TYR CYS ASP GLU LYS GLY VAL TRP PHE LYS HIS PRO          
SEQRES  11 R  501  GLU ASN ASN ARG THR TRP SER ASN TYR THR MET CYS ASN          
SEQRES  12 R  501  ALA PHE THR PRO GLU LYS LEU LYS ASN ALA TYR VAL LEU          
SEQRES  13 R  501  TYR TYR LEU ALA ILE VAL GLY HIS SER LEU SER ILE PHE          
SEQRES  14 R  501  THR LEU VAL ILE SER LEU GLY ILE PHE VAL PHE PHE ARG          
SEQRES  15 R  501  SER LEU GLY CYS GLN ARG VAL THR LEU HIS LYS ASN MET          
SEQRES  16 R  501  PHE LEU THR TYR ILE LEU ASN SER MET ILE ILE ILE ILE          
SEQRES  17 R  501  HIS LEU VAL GLU VAL VAL PRO ASN GLY GLU LEU VAL ARG          
SEQRES  18 R  501  ARG ASP PRO VAL SER CYS LYS ILE LEU HIS PHE PHE HIS          
SEQRES  19 R  501  GLN TYR MET MET ALA CYS ASN TYR PHE TRP MET LEU CYS          
SEQRES  20 R  501  GLU GLY ILE TYR LEU HIS THR LEU ILE VAL VAL ALA VAL          
SEQRES  21 R  501  PHE THR GLU LYS GLN ARG LEU ARG TRP TYR TYR LEU LEU          
SEQRES  22 R  501  GLY TRP GLY PHE PRO LEU VAL PRO THR THR ILE HIS ALA          
SEQRES  23 R  501  ILE THR ARG ALA VAL TYR PHE ASN ASP ASN CYS TRP LEU          
SEQRES  24 R  501  SER VAL GLU THR HIS LEU LEU TYR ILE ILE HIS GLY PRO          
SEQRES  25 R  501  VAL MET ALA ALA LEU VAL VAL ASN PHE PHE PHE LEU LEU          
SEQRES  26 R  501  ASN ILE VAL ARG VAL LEU VAL THR LYS MET ARG GLU THR          
SEQRES  27 R  501  HIS GLU ALA GLU SER HIS MET TYR LEU LYS ALA VAL LYS          
SEQRES  28 R  501  ALA THR MET ILE LEU VAL PRO LEU LEU GLY ILE GLN PHE          
SEQRES  29 R  501  VAL VAL PHE PRO TRP ARG PRO SER ASN LYS MET LEU GLY          
SEQRES  30 R  501  LYS ILE TYR ASP TYR VAL MET HIS SER LEU ILE HIS PHE          
SEQRES  31 R  501  GLN GLY PHE PHE VAL ALA THR ILE TYR CYS PHE CYS ASN          
SEQRES  32 R  501  ASN GLU VAL GLN THR THR VAL LYS ARG GLN TRP ALA GLN          
SEQRES  33 R  501  PHE LYS ILE GLN TRP ASN GLN ARG TRP GLY ARG ARG PRO          
SEQRES  34 R  501  SER ASN ARG SER ALA ARG ALA ALA ALA ALA ALA ALA GLU          
SEQRES  35 R  501  ALA GLY ASP ILE PRO ILE TYR ILE CYS HIS GLN GLU LEU          
SEQRES  36 R  501  ARG ASN GLU PRO ALA ASN ASN GLN GLY GLU GLU SER ALA          
SEQRES  37 R  501  GLU ILE ILE PRO LEU ASN ILE ILE GLU GLN GLU SER SER          
SEQRES  38 R  501  ALA PRO ALA GLY LEU GLU VAL LEU PHE GLN GLY PRO HIS          
SEQRES  39 R  501  HIS HIS HIS HIS HIS HIS HIS                                  
HELIX    1 AA1 THR A    9  ALA A   39  1                                  31    
HELIX    2 AA2 ARG A  265  ASN A  278  1                                  14    
HELIX    3 AA3 PHE A  312  ALA A  316  5                                   5    
HELIX    4 AA4 PRO A  332  GLY A  353  1                                  22    
HELIX    5 AA5 ASN A  371  TYR A  391  1                                  21    
HELIX    6 AA6 SER B    2  CYS B   25  1                                  24    
HELIX    7 AA7 THR B   29  THR B   34  1                                   6    
HELIX    8 AA8 ALA G   10  ARG G   13  5                                   4    
HELIX    9 AA9 LYS G   14  ASN G   24  1                                  11    
HELIX   10 AB1 LYS G   29  HIS G   44  1                                  16    
HELIX   11 AB2 THR N   28  TYR N   32  5                                   5    
HELIX   12 AB3 LYS N   87  THR N   91  5                                   5    
HELIX   13 AB4 PHE R  137  PHE R  173  1                                  37    
HELIX   14 AB5 CYS R  178  VAL R  205  1                                  28    
HELIX   15 AB6 ASP R  215  VAL R  250  1                                  36    
HELIX   16 AB7 ARG R  258  GLY R  268  1                                  11    
HELIX   17 AB8 PRO R  270  PHE R  285  1                                  16    
HELIX   18 AB9 LEU R  298  LYS R  326  1                                  29    
HELIX   19 AC1 TYR R  338  VAL R  349  1                                  12    
HELIX   20 AC2 PRO R  350  GLY R  353  5                                   4    
HELIX   21 AC3 ILE R  354  PHE R  359  1                                   6    
HELIX   22 AC4 MET R  367  PHE R  382  1                                  16    
HELIX   23 AC5 PHE R  382  PHE R  393  1                                  12    
HELIX   24 AC6 ASN R  395  GLY R  418  1                                  24    
SHEET    1 AA1 6 THR A 210  VAL A 214  0                                        
SHEET    2 AA1 6 VAL A 217  ASP A 223 -1  O  MET A 221   N  THR A 210           
SHEET    3 AA1 6 THR A  40  LEU A  46  1  N  HIS A  41   O  ASN A 218           
SHEET    4 AA1 6 ALA A 243  VAL A 248  1  O  ILE A 245   N  LEU A  44           
SHEET    5 AA1 6 SER A 286  LEU A 291  1  O  PHE A 290   N  VAL A 248           
SHEET    6 AA1 6 CYS A 359  PRO A 361  1  O  TYR A 360   N  LEU A 289           
SHEET    1 AA2 4 THR B  47  LEU B  51  0                                        
SHEET    2 AA2 4 LEU B 336  TRP B 339 -1  O  LEU B 336   N  LEU B  51           
SHEET    3 AA2 4 VAL B 327  SER B 331 -1  N  VAL B 327   O  TRP B 339           
SHEET    4 AA2 4 VAL B 315  VAL B 320 -1  N  CYS B 317   O  GLY B 330           
SHEET    1 AA3 4 MET B  61  HIS B  62  0                                        
SHEET    2 AA3 4 LEU B  69  SER B  72 -1  O  VAL B  71   N  HIS B  62           
SHEET    3 AA3 4 LYS B  78  ASP B  83 -1  O  TRP B  82   N  LEU B  70           
SHEET    4 AA3 4 LYS B  89  PRO B  94 -1  O  ILE B  93   N  LEU B  79           
SHEET    1 AA4 4 VAL B 100  TYR B 105  0                                        
SHEET    2 AA4 4 TYR B 111  GLY B 116 -1  O  GLY B 115   N  THR B 102           
SHEET    3 AA4 4 CYS B 121  ASN B 125 -1  O  TYR B 124   N  VAL B 112           
SHEET    4 AA4 4 ARG B 134  LEU B 139 -1  O  ARG B 137   N  ILE B 123           
SHEET    1 AA5 4 CYS B 149  PHE B 151  0                                        
SHEET    2 AA5 4 GLN B 156  THR B 159 -1  O  VAL B 158   N  ARG B 150           
SHEET    3 AA5 4 CYS B 166  ASP B 170 -1  O  ALA B 167   N  THR B 159           
SHEET    4 AA5 4 GLN B 176  PHE B 180 -1  O  PHE B 180   N  CYS B 166           
SHEET    1 AA6 4 LEU B 190  LEU B 192  0                                        
SHEET    2 AA6 4 LEU B 198  SER B 201 -1  O  VAL B 200   N  SER B 191           
SHEET    3 AA6 4 SER B 207  ASP B 212 -1  O  LYS B 209   N  SER B 201           
SHEET    4 AA6 4 CYS B 218  THR B 223 -1  O  PHE B 222   N  ALA B 208           
SHEET    1 AA7 4 ILE B 232  PHE B 234  0                                        
SHEET    2 AA7 4 ALA B 240  THR B 243 -1  O  ALA B 242   N  CYS B 233           
SHEET    3 AA7 4 CYS B 250  ASP B 254 -1  O  ARG B 251   N  THR B 243           
SHEET    4 AA7 4 GLU B 260  TYR B 264 -1  O  TYR B 264   N  CYS B 250           
SHEET    1 AA8 4 SER B 275  PHE B 278  0                                        
SHEET    2 AA8 4 LEU B 284  GLY B 288 -1  O  GLY B 288   N  SER B 275           
SHEET    3 AA8 4 CYS B 294  ASP B 298 -1  O  ASN B 295   N  ALA B 287           
SHEET    4 AA8 4 ARG B 304  LEU B 308 -1  O  GLY B 306   N  VAL B 296           
SHEET    1 AA9 4 GLN N   3  GLY N   8  0                                        
SHEET    2 AA9 4 ARG N  19  SER N  25 -1  O  ALA N  23   N  GLN N   5           
SHEET    3 AA9 4 THR N  78  MET N  83 -1  O  LEU N  79   N  CYS N  22           
SHEET    4 AA9 4 PHE N  68  ASP N  73 -1  N  SER N  71   O  TYR N  80           
SHEET    1 AB1 6 LEU N  11  VAL N  12  0                                        
SHEET    2 AB1 6 THR N 122  VAL N 126  1  O  THR N 125   N  VAL N  12           
SHEET    3 AB1 6 ALA N  92  ARG N  98 -1  N  TYR N  94   O  THR N 122           
SHEET    4 AB1 6 MET N  34  GLN N  39 -1  N  VAL N  37   O  TYR N  95           
SHEET    5 AB1 6 LEU N  45  ILE N  51 -1  O  GLU N  46   N  ARG N  38           
SHEET    6 AB1 6 ILE N  58  SER N  59 -1  O  SER N  59   N  ASP N  50           
SSBOND   1 CYS N   22    CYS N   96                          1555   1555  2.03  
SSBOND   2 CYS N   99    CYS N  107                          1555   1555  2.02  
SSBOND   3 CYS R  219    CYS R  289                          1555   1555  2.04  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1                      
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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