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Database: PDB
Entry: 5VAI
LinkDB: 5VAI
Original site: 5VAI 
HEADER    SIGNALING PROTEIN/HORMONE               27-MAR-17   5VAI              
TITLE     CRYO-EM STRUCTURE OF THE ACTIVATED GLUCAGON-LIKE PEPTIDE-1 RECEPTOR IN
TITLE    2 COMPLEX WITH G PROTEIN                                               
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: UNCHARACTERIZED PROTEIN;                                   
COMPND   3 CHAIN: R;                                                            
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: GLUCAGON-LIKE PEPTIDE 1;                                   
COMPND   7 CHAIN: P;                                                            
COMPND   8 FRAGMENT: UNP RESIDUES 98-128;                                       
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MOL_ID: 3;                                                           
COMPND  11 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(S) SUBUNIT ALPHA      
COMPND  12 ISOFORMS SHORT;                                                      
COMPND  13 CHAIN: A;                                                            
COMPND  14 FRAGMENT: UNP RESIDUEWS 1-380;                                       
COMPND  15 SYNONYM: ADENYLATE CYCLASE-STIMULATING G ALPHA PROTEIN;              
COMPND  16 ENGINEERED: YES;                                                     
COMPND  17 MOL_ID: 4;                                                           
COMPND  18 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) SUBUNIT  
COMPND  19 BETA-1;                                                              
COMPND  20 CHAIN: B;                                                            
COMPND  21 SYNONYM: TRANSDUCIN BETA CHAIN 1;                                    
COMPND  22 ENGINEERED: YES;                                                     
COMPND  23 MOL_ID: 5;                                                           
COMPND  24 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(O) SUBUNIT  
COMPND  25 GAMMA-2;                                                             
COMPND  26 CHAIN: G;                                                            
COMPND  27 FRAGMENT: UNP RESIDUES 1-68;                                         
COMPND  28 SYNONYM: G GAMMA-I;                                                  
COMPND  29 ENGINEERED: YES;                                                     
COMPND  30 MOL_ID: 6;                                                           
COMPND  31 MOLECULE: NANOBODY 35;                                               
COMPND  32 CHAIN: N;                                                            
COMPND  33 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ORYCTOLAGUS CUNICULUS;                          
SOURCE   3 ORGANISM_COMMON: RABBIT;                                             
SOURCE   4 ORGANISM_TAXID: 9986;                                                
SOURCE   5 GENE: GLP1R;                                                         
SOURCE   6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;                            
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 7108;                                       
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 SYNTHETIC: YES;                                                      
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 MOL_ID: 3;                                                           
SOURCE  14 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  15 ORGANISM_COMMON: HUMAN;                                              
SOURCE  16 ORGANISM_TAXID: 9606;                                                
SOURCE  17 GENE: GNAS, GNAS1, GSP;                                              
SOURCE  18 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  19 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  20 MOL_ID: 4;                                                           
SOURCE  21 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE  22 ORGANISM_COMMON: RAT;                                                
SOURCE  23 ORGANISM_TAXID: 10116;                                               
SOURCE  24 GENE: GNB1;                                                          
SOURCE  25 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  26 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  27 MOL_ID: 5;                                                           
SOURCE  28 ORGANISM_SCIENTIFIC: BOS TAURUS;                                     
SOURCE  29 ORGANISM_COMMON: BOVINE;                                             
SOURCE  30 ORGANISM_TAXID: 9913;                                                
SOURCE  31 GENE: GNG2;                                                          
SOURCE  32 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
SOURCE  33 EXPRESSION_SYSTEM_TAXID: 7111;                                       
SOURCE  34 MOL_ID: 6;                                                           
SOURCE  35 ORGANISM_SCIENTIFIC: LAMA GLAMA;                                     
SOURCE  36 ORGANISM_TAXID: 9844;                                                
SOURCE  37 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  38 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    CLASS B GPCR, GLP-1, GLP-1R, COMPLEX, SIGNALING PROTEIN-HORMONE       
KEYWDS   2 COMPLEX                                                              
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    Y.ZHANG,B.SUN,D.FENG,H.HU,M.CHU,Q.QU,J.T.TARRASCH,S.LI,T.S.KOBILKA,   
AUTHOR   2 B.K.KOBILKA,G.SKINIOTIS                                              
REVDAT   5   18-DEC-19 5VAI    1       CRYST1 SCALE                             
REVDAT   4   18-JUL-18 5VAI    1       REMARK                                   
REVDAT   3   21-JUN-17 5VAI    1       JRNL                                     
REVDAT   2   07-JUN-17 5VAI    1       JRNL                                     
REVDAT   1   24-MAY-17 5VAI    0                                                
JRNL        AUTH   Y.ZHANG,B.SUN,D.FENG,H.HU,M.CHU,Q.QU,J.T.TARRASCH,S.LI,      
JRNL        AUTH 2 T.SUN KOBILKA,B.K.KOBILKA,G.SKINIOTIS                        
JRNL        TITL   CRYO-EM STRUCTURE OF THE ACTIVATED GLP-1 RECEPTOR IN COMPLEX 
JRNL        TITL 2 WITH A G PROTEIN.                                            
JRNL        REF    NATURE                        V. 546   248 2017              
JRNL        REFN                   ESSN 1476-4687                               
JRNL        PMID   28538729                                                     
JRNL        DOI    10.1038/NATURE22394                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    4.10 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : RELION, RELION                            
REMARK   3   RECONSTRUCTION SCHEMA  : BACK PROJECTION                           
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : NULL                                
REMARK   3   REFINEMENT SPACE             : NULL                                
REMARK   3   REFINEMENT PROTOCOL          : NULL                                
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : NULL                                             
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 4.100                          
REMARK   3   NUMBER OF PARTICLES               : 139299                         
REMARK   3   CTF CORRECTION METHOD             : PHASE FLIPPING AND AMPLITUDE   
REMARK   3                                       CORRECTION                     
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 5VAI COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 17-APR-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000226989.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : NULL                              
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : THE ACTIVATED GLUCAGON-LIKE       
REMARK 245                                    PEPTIDE-1 RECEPTOR IN COMPLEX     
REMARK 245                                    WITH THE HETEROTRIMERIC GS        
REMARK 245                                    PROTEIN                           
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 1.00                              
REMARK 245   SAMPLE SUPPORT DETAILS         : NULL                              
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : NULL                           
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : NULL                           
REMARK 245   MICROSCOPE MODEL                  : FEI TITAN KRIOS                
REMARK 245   DETECTOR TYPE                     : GATAN K2 SUMMIT (4K X 4K)      
REMARK 245   MINIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MAXIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : NULL                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 90.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : 29000                          
REMARK 245   CALIBRATED MAGNIFICATION          : 50000                          
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 300                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: HEXAMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: R, P, A, B, G, N                      
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET R   -38                                                      
REMARK 465     LYS R   -37                                                      
REMARK 465     THR R   -36                                                      
REMARK 465     ILE R   -35                                                      
REMARK 465     ILE R   -34                                                      
REMARK 465     ALA R   -33                                                      
REMARK 465     LEU R   -32                                                      
REMARK 465     SER R   -31                                                      
REMARK 465     TYR R   -30                                                      
REMARK 465     ILE R   -29                                                      
REMARK 465     PHE R   -28                                                      
REMARK 465     CYS R   -27                                                      
REMARK 465     LEU R   -26                                                      
REMARK 465     VAL R   -25                                                      
REMARK 465     PHE R   -24                                                      
REMARK 465     ALA R   -23                                                      
REMARK 465     ASP R   -22                                                      
REMARK 465     TYR R   -21                                                      
REMARK 465     LYS R   -20                                                      
REMARK 465     ASP R   -19                                                      
REMARK 465     ASP R   -18                                                      
REMARK 465     ASP R   -17                                                      
REMARK 465     ASP R   -16                                                      
REMARK 465     ALA R   -15                                                      
REMARK 465     ALA R   -14                                                      
REMARK 465     ALA R   -13                                                      
REMARK 465     GLY R   -12                                                      
REMARK 465     GLN R   -11                                                      
REMARK 465     PRO R   -10                                                      
REMARK 465     GLY R    -9                                                      
REMARK 465     ASN R    -8                                                      
REMARK 465     GLY R    -7                                                      
REMARK 465     SER R    -6                                                      
REMARK 465     ALA R    -5                                                      
REMARK 465     PHE R    -4                                                      
REMARK 465     LEU R    -3                                                      
REMARK 465     LEU R    -2                                                      
REMARK 465     ALA R    -1                                                      
REMARK 465     PRO R     0                                                      
REMARK 465     ASN R     1                                                      
REMARK 465     ARG R     2                                                      
REMARK 465     SER R     3                                                      
REMARK 465     HIS R     4                                                      
REMARK 465     ALA R     5                                                      
REMARK 465     PRO R     6                                                      
REMARK 465     GLY R     7                                                      
REMARK 465     GLY R     8                                                      
REMARK 465     GLY R     9                                                      
REMARK 465     GLY R    10                                                      
REMARK 465     SER R    11                                                      
REMARK 465     LEU R    12                                                      
REMARK 465     GLU R    13                                                      
REMARK 465     VAL R    14                                                      
REMARK 465     LEU R    15                                                      
REMARK 465     PHE R    16                                                      
REMARK 465     GLN R    17                                                      
REMARK 465     GLY R    18                                                      
REMARK 465     PRO R    19                                                      
REMARK 465     GLY R    20                                                      
REMARK 465     GLY R    21                                                      
REMARK 465     GLY R    22                                                      
REMARK 465     SER R    23                                                      
REMARK 465     ARG R    24                                                      
REMARK 465     PRO R    25                                                      
REMARK 465     GLN R    26                                                      
REMARK 465     GLY R    27                                                      
REMARK 465     ALA R    28                                                      
REMARK 465     SER R   129                                                      
REMARK 465     ARG R   130                                                      
REMARK 465     ARG R   131                                                      
REMARK 465     GLY R   132                                                      
REMARK 465     GLU R   133                                                      
REMARK 465     LYS R   134                                                      
REMARK 465     LEU R   422                                                      
REMARK 465     MET A     1                                                      
REMARK 465     GLY A     2                                                      
REMARK 465     CYS A     3                                                      
REMARK 465     LEU A     4                                                      
REMARK 465     GLY A     5                                                      
REMARK 465     ASN A     6                                                      
REMARK 465     SER A     7                                                      
REMARK 465     LYS A     8                                                      
REMARK 465     MET A    74                                                      
REMARK 465     ARG A    75                                                      
REMARK 465     ILE A    76                                                      
REMARK 465     LEU A    77                                                      
REMARK 465     HIS A    78                                                      
REMARK 465     VAL A    79                                                      
REMARK 465     ASN A    80                                                      
REMARK 465     GLY A    81                                                      
REMARK 465     PHE A    82                                                      
REMARK 465     ASN A    83                                                      
REMARK 465     GLY A    84                                                      
REMARK 465     ASP A    85                                                      
REMARK 465     SER A    86                                                      
REMARK 465     GLU A    87                                                      
REMARK 465     LYS A    88                                                      
REMARK 465     ALA A    89                                                      
REMARK 465     THR A    90                                                      
REMARK 465     LYS A    91                                                      
REMARK 465     VAL A    92                                                      
REMARK 465     GLN A    93                                                      
REMARK 465     ASP A    94                                                      
REMARK 465     ILE A    95                                                      
REMARK 465     LYS A    96                                                      
REMARK 465     ASN A    97                                                      
REMARK 465     ASN A    98                                                      
REMARK 465     LEU A    99                                                      
REMARK 465     LYS A   100                                                      
REMARK 465     GLU A   101                                                      
REMARK 465     ALA A   102                                                      
REMARK 465     ILE A   103                                                      
REMARK 465     GLU A   104                                                      
REMARK 465     THR A   105                                                      
REMARK 465     ILE A   106                                                      
REMARK 465     VAL A   107                                                      
REMARK 465     ALA A   108                                                      
REMARK 465     ALA A   109                                                      
REMARK 465     MET A   110                                                      
REMARK 465     SER A   111                                                      
REMARK 465     ASN A   112                                                      
REMARK 465     LEU A   113                                                      
REMARK 465     VAL A   114                                                      
REMARK 465     PRO A   115                                                      
REMARK 465     PRO A   116                                                      
REMARK 465     VAL A   117                                                      
REMARK 465     GLU A   118                                                      
REMARK 465     LEU A   119                                                      
REMARK 465     ALA A   120                                                      
REMARK 465     ASN A   121                                                      
REMARK 465     PRO A   122                                                      
REMARK 465     GLU A   123                                                      
REMARK 465     ASN A   124                                                      
REMARK 465     GLN A   125                                                      
REMARK 465     PHE A   126                                                      
REMARK 465     ARG A   127                                                      
REMARK 465     VAL A   128                                                      
REMARK 465     ASP A   129                                                      
REMARK 465     TYR A   130                                                      
REMARK 465     ILE A   131                                                      
REMARK 465     LEU A   132                                                      
REMARK 465     SER A   133                                                      
REMARK 465     VAL A   134                                                      
REMARK 465     MET A   135                                                      
REMARK 465     ASN A   136                                                      
REMARK 465     VAL A   137                                                      
REMARK 465     PRO A   138                                                      
REMARK 465     ASP A   139                                                      
REMARK 465     PHE A   140                                                      
REMARK 465     ASP A   141                                                      
REMARK 465     PHE A   142                                                      
REMARK 465     PRO A   143                                                      
REMARK 465     PRO A   144                                                      
REMARK 465     GLU A   145                                                      
REMARK 465     PHE A   146                                                      
REMARK 465     TYR A   147                                                      
REMARK 465     GLU A   148                                                      
REMARK 465     HIS A   149                                                      
REMARK 465     ALA A   150                                                      
REMARK 465     LYS A   151                                                      
REMARK 465     ALA A   152                                                      
REMARK 465     LEU A   153                                                      
REMARK 465     TRP A   154                                                      
REMARK 465     GLU A   155                                                      
REMARK 465     ASP A   156                                                      
REMARK 465     GLU A   157                                                      
REMARK 465     GLY A   158                                                      
REMARK 465     VAL A   159                                                      
REMARK 465     ARG A   160                                                      
REMARK 465     ALA A   161                                                      
REMARK 465     CYS A   162                                                      
REMARK 465     TYR A   163                                                      
REMARK 465     GLU A   164                                                      
REMARK 465     ARG A   165                                                      
REMARK 465     SER A   166                                                      
REMARK 465     ASN A   167                                                      
REMARK 465     GLU A   168                                                      
REMARK 465     TYR A   169                                                      
REMARK 465     GLN A   170                                                      
REMARK 465     LEU A   171                                                      
REMARK 465     ILE A   172                                                      
REMARK 465     ASP A   173                                                      
REMARK 465     CYS A   174                                                      
REMARK 465     ALA A   175                                                      
REMARK 465     GLN A   176                                                      
REMARK 465     TYR A   177                                                      
REMARK 465     PHE A   178                                                      
REMARK 465     LEU A   179                                                      
REMARK 465     ASP A   180                                                      
REMARK 465     LYS A   181                                                      
REMARK 465     ILE A   182                                                      
REMARK 465     ASP A   183                                                      
REMARK 465     VAL A   184                                                      
REMARK 465     ILE A   185                                                      
REMARK 465     LYS A   186                                                      
REMARK 465     GLN A   187                                                      
REMARK 465     ASP A   188                                                      
REMARK 465     ASP A   189                                                      
REMARK 465     TYR A   190                                                      
REMARK 465     VAL A   191                                                      
REMARK 465     PRO A   192                                                      
REMARK 465     SER A   193                                                      
REMARK 465     ASP A   194                                                      
REMARK 465     GLN A   195                                                      
REMARK 465     ASP A   196                                                      
REMARK 465     LEU A   197                                                      
REMARK 465     LEU A   198                                                      
REMARK 465     ARG A   199                                                      
REMARK 465     CYS A   200                                                      
REMARK 465     ARG A   201                                                      
REMARK 465     VAL A   202                                                      
REMARK 465     LEU A   203                                                      
REMARK 465     THR A   204                                                      
REMARK 465     VAL A   256                                                      
REMARK 465     ILE A   257                                                      
REMARK 465     ARG A   258                                                      
REMARK 465     GLU A   259                                                      
REMARK 465     ASP A   260                                                      
REMARK 465     ASN A   261                                                      
REMARK 465     GLN A   262                                                      
REMARK 465     MET B   -10                                                      
REMARK 465     HIS B    -9                                                      
REMARK 465     HIS B    -8                                                      
REMARK 465     HIS B    -7                                                      
REMARK 465     HIS B    -6                                                      
REMARK 465     HIS B    -5                                                      
REMARK 465     HIS B    -4                                                      
REMARK 465     GLY B    -3                                                      
REMARK 465     SER B    -2                                                      
REMARK 465     LEU B    -1                                                      
REMARK 465     LEU B     0                                                      
REMARK 465     MET G     1                                                      
REMARK 465     ALA G     2                                                      
REMARK 465     SER G     3                                                      
REMARK 465     ASN G     4                                                      
REMARK 465     GLU G    63                                                      
REMARK 465     LYS G    64                                                      
REMARK 465     LYS G    65                                                      
REMARK 465     PHE G    66                                                      
REMARK 465     PHE G    67                                                      
REMARK 465     CYS G    68                                                      
REMARK 465     HIS N   129                                                      
REMARK 465     HIS N   130                                                      
REMARK 465     HIS N   131                                                      
REMARK 465     HIS N   132                                                      
REMARK 465     HIS N   133                                                      
REMARK 465     HIS N   134                                                      
REMARK 465     GLU N   135                                                      
REMARK 465     PRO N   136                                                      
REMARK 465     GLU N   137                                                      
REMARK 465     ALA N   138                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LEU R  56    CG   CD1  CD2                                       
REMARK 470     THR R  58    OG1  CG2                                            
REMARK 470     LEU R  60    CG   CD1  CD2                                       
REMARK 470     PHE R  61    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ASN R  63    CG   OD1  ND2                                       
REMARK 470     ARG R  64    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     THR R  65    OG1  CG2                                            
REMARK 470     PHE R  66    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ASP R  67    CG   OD1  OD2                                       
REMARK 470     ASP R  68    CG   OD1  OD2                                       
REMARK 470     TYR R  69    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     TRP R  72    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP R  72    CZ3  CH2                                            
REMARK 470     ASP R  74    CG   OD1  OD2                                       
REMARK 470     SER R  79    OG                                                  
REMARK 470     PHE R  80    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     VAL R  81    CG1  CG2                                            
REMARK 470     ASN R  82    CG   OD1  ND2                                       
REMARK 470     VAL R  83    CG1  CG2                                            
REMARK 470     SER R  84    OG                                                  
REMARK 470     LEU R  89    CG   CD1  CD2                                       
REMARK 470     SER R  93    OG                                                  
REMARK 470     ASN R  94    CG   OD1  ND2                                       
REMARK 470     VAL R  95    CG1  CG2                                            
REMARK 470     LEU R  96    CG   CD1  CD2                                       
REMARK 470     GLN R  97    CG   CD   OE1  NE2                                  
REMARK 470     HIS R  99    CG   ND1  CD2  CE1  NE2                             
REMARK 470     VAL R 100    CG1  CG2                                            
REMARK 470     TYR R 101    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ARG R 102    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     PHE R 103    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     THR R 105    OG1  CG2                                            
REMARK 470     GLU R 107    CG   CD   OE1  OE2                                  
REMARK 470     HIS R 109    CG   ND1  CD2  CE1  NE2                             
REMARK 470     TRP R 110    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP R 110    CZ3  CH2                                            
REMARK 470     LEU R 111    CG   CD1  CD2                                       
REMARK 470     PRO R 112    CG   CD                                             
REMARK 470     ASP R 114    CG   OD1  OD2                                       
REMARK 470     ASN R 115    CB   CG   OD1  ND2                                  
REMARK 470     SER R 116    OG                                                  
REMARK 470     SER R 117    OG                                                  
REMARK 470     ASP R 122    CG   OD1  OD2                                       
REMARK 470     SER R 124    OG                                                  
REMARK 470     GLU R 125    CG   CD   OE1  OE2                                  
REMARK 470     GLU R 127    CG   CD   OE1  OE2                                  
REMARK 470     GLN R 210    CG   CD   OE1  NE2                                  
REMARK 470     ARG R 227    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG R 264    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     MET R 303    CG   SD   CE                                        
REMARK 470     ARG R 310    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     PHE R 367    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     MET R 371    CG   SD   CE                                        
REMARK 470     ASP R 372    CG   OD1  OD2                                       
REMARK 470     ARG R 376    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG R 380    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG P  36    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A  24    CG   CD   CE   NZ                                   
REMARK 470     LYS A  58    CG   CD   CE   NZ                                   
REMARK 470     GLN A  59    CG   CD   OE1  NE2                                  
REMARK 470     PHE A 212    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LYS A 216    CG   CD   CE   NZ                                   
REMARK 470     ASP A 240    CG   OD1  OD2                                       
REMARK 470     LYS A 300    CG   CD   CE   NZ                                   
REMARK 470     GLU A 322    CG   CD   OE1  OE2                                  
REMARK 470     THR A 369    OG1  CG2                                            
REMARK 470     GLN B   1    CG   CD   OE1  NE2                                  
REMARK 470     ARG B  42    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 129    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 130    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 172    CG   CD   OE1  OE2                                  
REMARK 470     PHE B 234    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ARG G  62    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER N 112    OG                                                  
REMARK 470     THR N 113    OG1  CG2                                            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OG1  THR A   325     OH   TYR A   339              2.05            
REMARK 500   O    ASP N    90     OH   TYR N    94              2.07            
REMARK 500   OE1  GLN B   176     OG1  THR B   179              2.10            
REMARK 500   OG1  THR R   175     OH   TYR R   250              2.11            
REMARK 500   O    TYR A   318     NH2  ARG A   336              2.12            
REMARK 500   OG1  THR B   128     O    GLY B   131              2.13            
REMARK 500   O    GLU R   139     N    SER R   143              2.13            
REMARK 500   O    ASP B   228     OG   SER B   245              2.16            
REMARK 500   NZ   LYS R   197     OG1  THR P    13              2.16            
REMARK 500   O    LEU A   291     NE2  GLN A   294              2.17            
REMARK 500   OH   TYR A   253     O    LYS A   300              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU R 166   CA  -  CB  -  CG  ANGL. DEV. = -14.5 DEGREES          
REMARK 500    ALA N 101   C   -  N   -  CA  ANGL. DEV. = -16.3 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG R  64      151.51    -39.75                                   
REMARK 500    ASP R  68       -6.26     81.52                                   
REMARK 500    ASN R 115        3.16     80.69                                   
REMARK 500    ARG R 170      -10.62     77.33                                   
REMARK 500    GLU R 294       35.96    -99.21                                   
REMARK 500    TRP R 297      130.72    104.04                                   
REMARK 500    MET R 303       -1.87     69.00                                   
REMARK 500    CYS R 341       -1.74    -56.51                                   
REMARK 500    MET R 371     -149.29   -115.73                                   
REMARK 500    ARG R 376     -129.15     61.15                                   
REMARK 500    ASN A 254      -75.09    -81.62                                   
REMARK 500    GLU A 314       -4.20    -59.74                                   
REMARK 500    CYS A 365       -0.83     85.57                                   
REMARK 500    PHE A 376      -73.19    -53.27                                   
REMARK 500    GLU A 392       17.78     54.56                                   
REMARK 500    CYS B  25        0.75    -67.81                                   
REMARK 500    ARG B  68      -68.87    -96.61                                   
REMARK 500    THR B  87       29.14     44.17                                   
REMARK 500    ASN B 132     -171.27    -68.00                                   
REMARK 500    ASP B 153     -165.98   -160.42                                   
REMARK 500    ASN B 155      -62.23   -108.79                                   
REMARK 500    THR B 164       -7.30     84.16                                   
REMARK 500    PHE B 292        9.49     81.87                                   
REMARK 500    ALA B 302       -3.30     79.08                                   
REMARK 500    ALA B 309     -178.21    -69.48                                   
REMARK 500    SER B 334       -4.29     72.57                                   
REMARK 500    PHE N  27      160.01    179.48                                   
REMARK 500    ALA N  56      -65.99    -90.56                                   
REMARK 500    ASP N 109     -166.42    -75.39                                   
REMARK 500    THR N 113     -119.06     58.04                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-8653   RELATED DB: EMDB                              
REMARK 900 CRYO-EM STRUCTURE OF THE ACTIVATED GLUCAGON-LIKE PEPTIDE-1 RECEPTOR  
REMARK 900 IN COMPLEX WITH G PROTEIN                                            
DBREF  5VAI R   24   422  UNP    G1SGD4   G1SGD4_RABIT    24    422             
DBREF  5VAI P    7    37  UNP    P01275   GLUC_HUMAN      98    128             
DBREF  5VAI A    1   394  UNP    P63092   GNAS2_HUMAN      1    380             
DBREF  5VAI B    2   340  UNP    P54311   GBB1_RAT         2    340             
DBREF  5VAI G    1    68  UNP    P63212   GBG2_BOVIN       1     68             
DBREF  5VAI N    1   138  PDB    5VAI     5VAI             1    138             
SEQADV 5VAI MET R  -38  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LYS R  -37  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI THR R  -36  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ILE R  -35  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ILE R  -34  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R  -33  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LEU R  -32  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI SER R  -31  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI TYR R  -30  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ILE R  -29  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PHE R  -28  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI CYS R  -27  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LEU R  -26  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI VAL R  -25  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PHE R  -24  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R  -23  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASP R  -22  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI TYR R  -21  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LYS R  -20  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASP R  -19  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASP R  -18  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASP R  -17  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASP R  -16  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R  -15  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R  -14  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R  -13  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R  -12  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLN R  -11  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PRO R  -10  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   -9  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASN R   -8  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   -7  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI SER R   -6  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R   -5  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PHE R   -4  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LEU R   -3  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LEU R   -2  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R   -1  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PRO R    0  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ASN R    1  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ARG R    2  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI SER R    3  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI HIS R    4  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R    5  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PRO R    6  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R    7  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R    8  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R    9  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   10  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI SER R   11  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LEU R   12  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLU R   13  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI VAL R   14  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI LEU R   15  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PHE R   16  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLN R   17  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   18  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI PRO R   19  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   20  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   21  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI GLY R   22  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI SER R   23  UNP  G1SGD4              EXPRESSION TAG                 
SEQADV 5VAI ALA R  106  UNP  G1SGD4    THR   106 CONFLICT                       
SEQADV 5VAI PRO R  112  UNP  G1SGD4    HIS   112 CONFLICT                       
SEQADV 5VAI ARG R  140  UNP  G1SGD4    GLN   140 CONFLICT                       
SEQADV 5VAI ASP A  188  UNP  P63092    ALA   174 CONFLICT                       
SEQADV 5VAI MET B  -10  UNP  P54311              INITIATING METHIONINE          
SEQADV 5VAI HIS B   -9  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI HIS B   -8  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI HIS B   -7  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI HIS B   -6  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI HIS B   -5  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI HIS B   -4  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI GLY B   -3  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI SER B   -2  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI LEU B   -1  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI LEU B    0  UNP  P54311              EXPRESSION TAG                 
SEQADV 5VAI GLN B    1  UNP  P54311              EXPRESSION TAG                 
SEQRES   1 R  461  MET LYS THR ILE ILE ALA LEU SER TYR ILE PHE CYS LEU          
SEQRES   2 R  461  VAL PHE ALA ASP TYR LYS ASP ASP ASP ASP ALA ALA ALA          
SEQRES   3 R  461  GLY GLN PRO GLY ASN GLY SER ALA PHE LEU LEU ALA PRO          
SEQRES   4 R  461  ASN ARG SER HIS ALA PRO GLY GLY GLY GLY SER LEU GLU          
SEQRES   5 R  461  VAL LEU PHE GLN GLY PRO GLY GLY GLY SER ARG PRO GLN          
SEQRES   6 R  461  GLY ALA THR VAL SER LEU SER GLU THR VAL GLN LYS TRP          
SEQRES   7 R  461  ARG GLU TYR ARG ARG GLN CYS GLN HIS PHE LEU THR GLU          
SEQRES   8 R  461  ALA PRO PRO LEU ALA THR GLY LEU PHE CYS ASN ARG THR          
SEQRES   9 R  461  PHE ASP ASP TYR ALA CYS TRP PRO ASP GLY ALA PRO GLY          
SEQRES  10 R  461  SER PHE VAL ASN VAL SER CYS PRO TRP TYR LEU PRO TRP          
SEQRES  11 R  461  ALA SER ASN VAL LEU GLN GLY HIS VAL TYR ARG PHE CYS          
SEQRES  12 R  461  THR ALA GLU GLY HIS TRP LEU PRO LYS ASP ASN SER SER          
SEQRES  13 R  461  LEU PRO TRP ARG ASP LEU SER GLU CYS GLU GLU SER ARG          
SEQRES  14 R  461  ARG GLY GLU LYS SER SER PRO GLU GLU ARG LEU LEU SER          
SEQRES  15 R  461  LEU TYR ILE ILE TYR THR VAL GLY TYR ALA LEU SER PHE          
SEQRES  16 R  461  SER ALA LEU VAL ILE ALA SER ALA ILE LEU LEU GLY PHE          
SEQRES  17 R  461  ARG HIS LEU HIS CYS THR ARG ASN TYR ILE HIS LEU ASN          
SEQRES  18 R  461  LEU PHE ALA SER PHE ILE LEU ARG ALA LEU SER VAL PHE          
SEQRES  19 R  461  ILE LYS ASP ALA ALA LEU LYS TRP MET TYR SER THR ALA          
SEQRES  20 R  461  ALA GLN GLN HIS GLN TRP ASP GLY LEU LEU SER TYR GLN          
SEQRES  21 R  461  ASP SER LEU GLY CYS ARG LEU VAL PHE LEU LEU MET GLN          
SEQRES  22 R  461  TYR CYS VAL ALA ALA ASN TYR TYR TRP LEU LEU VAL GLU          
SEQRES  23 R  461  GLY ALA TYR LEU TYR THR LEU LEU ALA PHE ALA VAL PHE          
SEQRES  24 R  461  SER GLU GLN ARG ILE PHE LYS LEU TYR LEU SER ILE GLY          
SEQRES  25 R  461  TRP GLY VAL PRO LEU LEU PHE VAL ILE PRO TRP GLY ILE          
SEQRES  26 R  461  VAL LYS TYR LEU TYR GLU ASP GLU GLY CYS TRP THR ARG          
SEQRES  27 R  461  ASN SER ASN MET ASN TYR TRP LEU ILE ILE ARG LEU PRO          
SEQRES  28 R  461  ILE LEU PHE ALA ILE GLY VAL ASN PHE LEU ILE PHE ILE          
SEQRES  29 R  461  ARG VAL ILE CYS ILE VAL VAL SER LYS LEU LYS ALA ASN          
SEQRES  30 R  461  LEU MET CYS LYS THR ASP ILE LYS CYS ARG LEU ALA LYS          
SEQRES  31 R  461  SER THR LEU THR LEU ILE PRO LEU LEU GLY THR HIS GLU          
SEQRES  32 R  461  VAL ILE PHE ALA PHE VAL MET ASP GLU HIS ALA ARG GLY          
SEQRES  33 R  461  THR LEU ARG PHE VAL LYS LEU PHE THR GLU LEU SER PHE          
SEQRES  34 R  461  THR SER PHE GLN GLY LEU MET VAL ALA ILE LEU TYR CYS          
SEQRES  35 R  461  PHE VAL ASN ASN GLU VAL GLN MET GLU PHE ARG LYS SER          
SEQRES  36 R  461  TRP GLU ARG TRP ARG LEU                                      
SEQRES   1 P   31  HIS ALA GLU GLY THR PHE THR SER ASP VAL SER SER TYR          
SEQRES   2 P   31  LEU GLU GLY GLN ALA ALA LYS GLU PHE ILE ALA TRP LEU          
SEQRES   3 P   31  VAL LYS GLY ARG GLY                                          
SEQRES   1 A  380  MET GLY CYS LEU GLY ASN SER LYS THR GLU ASP GLN ARG          
SEQRES   2 A  380  ASN GLU GLU LYS ALA GLN ARG GLU ALA ASN LYS LYS ILE          
SEQRES   3 A  380  GLU LYS GLN LEU GLN LYS ASP LYS GLN VAL TYR ARG ALA          
SEQRES   4 A  380  THR HIS ARG LEU LEU LEU LEU GLY ALA GLY GLU SER GLY          
SEQRES   5 A  380  LYS SER THR ILE VAL LYS GLN MET ARG ILE LEU HIS VAL          
SEQRES   6 A  380  ASN GLY PHE ASN GLY ASP SER GLU LYS ALA THR LYS VAL          
SEQRES   7 A  380  GLN ASP ILE LYS ASN ASN LEU LYS GLU ALA ILE GLU THR          
SEQRES   8 A  380  ILE VAL ALA ALA MET SER ASN LEU VAL PRO PRO VAL GLU          
SEQRES   9 A  380  LEU ALA ASN PRO GLU ASN GLN PHE ARG VAL ASP TYR ILE          
SEQRES  10 A  380  LEU SER VAL MET ASN VAL PRO ASP PHE ASP PHE PRO PRO          
SEQRES  11 A  380  GLU PHE TYR GLU HIS ALA LYS ALA LEU TRP GLU ASP GLU          
SEQRES  12 A  380  GLY VAL ARG ALA CYS TYR GLU ARG SER ASN GLU TYR GLN          
SEQRES  13 A  380  LEU ILE ASP CYS ALA GLN TYR PHE LEU ASP LYS ILE ASP          
SEQRES  14 A  380  VAL ILE LYS GLN ASP ASP TYR VAL PRO SER ASP GLN ASP          
SEQRES  15 A  380  LEU LEU ARG CYS ARG VAL LEU THR SER GLY ILE PHE GLU          
SEQRES  16 A  380  THR LYS PHE GLN VAL ASP LYS VAL ASN PHE HIS MET PHE          
SEQRES  17 A  380  ASP VAL GLY GLY GLN ARG ASP GLU ARG ARG LYS TRP ILE          
SEQRES  18 A  380  GLN CYS PHE ASN ASP VAL THR ALA ILE ILE PHE VAL VAL          
SEQRES  19 A  380  ALA SER SER SER TYR ASN MET VAL ILE ARG GLU ASP ASN          
SEQRES  20 A  380  GLN THR ASN ARG LEU GLN GLU ALA LEU ASN LEU PHE LYS          
SEQRES  21 A  380  SER ILE TRP ASN ASN ARG TRP LEU ARG THR ILE SER VAL          
SEQRES  22 A  380  ILE LEU PHE LEU ASN LYS GLN ASP LEU LEU ALA GLU LYS          
SEQRES  23 A  380  VAL LEU ALA GLY LYS SER LYS ILE GLU ASP TYR PHE PRO          
SEQRES  24 A  380  GLU PHE ALA ARG TYR THR THR PRO GLU ASP ALA THR PRO          
SEQRES  25 A  380  GLU PRO GLY GLU ASP PRO ARG VAL THR ARG ALA LYS TYR          
SEQRES  26 A  380  PHE ILE ARG ASP GLU PHE LEU ARG ILE SER THR ALA SER          
SEQRES  27 A  380  GLY ASP GLY ARG HIS TYR CYS TYR PRO HIS PHE THR CYS          
SEQRES  28 A  380  ALA VAL ASP THR GLU ASN ILE ARG ARG VAL PHE ASN ASP          
SEQRES  29 A  380  CYS ARG ASP ILE ILE GLN ARG MET HIS LEU ARG GLN TYR          
SEQRES  30 A  380  GLU LEU LEU                                                  
SEQRES   1 B  351  MET HIS HIS HIS HIS HIS HIS GLY SER LEU LEU GLN SER          
SEQRES   2 B  351  GLU LEU ASP GLN LEU ARG GLN GLU ALA GLU GLN LEU LYS          
SEQRES   3 B  351  ASN GLN ILE ARG ASP ALA ARG LYS ALA CYS ALA ASP ALA          
SEQRES   4 B  351  THR LEU SER GLN ILE THR ASN ASN ILE ASP PRO VAL GLY          
SEQRES   5 B  351  ARG ILE GLN MET ARG THR ARG ARG THR LEU ARG GLY HIS          
SEQRES   6 B  351  LEU ALA LYS ILE TYR ALA MET HIS TRP GLY THR ASP SER          
SEQRES   7 B  351  ARG LEU LEU VAL SER ALA SER GLN ASP GLY LYS LEU ILE          
SEQRES   8 B  351  ILE TRP ASP SER TYR THR THR ASN LYS VAL HIS ALA ILE          
SEQRES   9 B  351  PRO LEU ARG SER SER TRP VAL MET THR CYS ALA TYR ALA          
SEQRES  10 B  351  PRO SER GLY ASN TYR VAL ALA CYS GLY GLY LEU ASP ASN          
SEQRES  11 B  351  ILE CYS SER ILE TYR ASN LEU LYS THR ARG GLU GLY ASN          
SEQRES  12 B  351  VAL ARG VAL SER ARG GLU LEU ALA GLY HIS THR GLY TYR          
SEQRES  13 B  351  LEU SER CYS CYS ARG PHE LEU ASP ASP ASN GLN ILE VAL          
SEQRES  14 B  351  THR SER SER GLY ASP THR THR CYS ALA LEU TRP ASP ILE          
SEQRES  15 B  351  GLU THR GLY GLN GLN THR THR THR PHE THR GLY HIS THR          
SEQRES  16 B  351  GLY ASP VAL MET SER LEU SER LEU ALA PRO ASP THR ARG          
SEQRES  17 B  351  LEU PHE VAL SER GLY ALA CYS ASP ALA SER ALA LYS LEU          
SEQRES  18 B  351  TRP ASP VAL ARG GLU GLY MET CYS ARG GLN THR PHE THR          
SEQRES  19 B  351  GLY HIS GLU SER ASP ILE ASN ALA ILE CYS PHE PHE PRO          
SEQRES  20 B  351  ASN GLY ASN ALA PHE ALA THR GLY SER ASP ASP ALA THR          
SEQRES  21 B  351  CYS ARG LEU PHE ASP LEU ARG ALA ASP GLN GLU LEU MET          
SEQRES  22 B  351  THR TYR SER HIS ASP ASN ILE ILE CYS GLY ILE THR SER          
SEQRES  23 B  351  VAL SER PHE SER LYS SER GLY ARG LEU LEU LEU ALA GLY          
SEQRES  24 B  351  TYR ASP ASP PHE ASN CYS ASN VAL TRP ASP ALA LEU LYS          
SEQRES  25 B  351  ALA ASP ARG ALA GLY VAL LEU ALA GLY HIS ASP ASN ARG          
SEQRES  26 B  351  VAL SER CYS LEU GLY VAL THR ASP ASP GLY MET ALA VAL          
SEQRES  27 B  351  ALA THR GLY SER TRP ASP SER PHE LEU LYS ILE TRP ASN          
SEQRES   1 G   68  MET ALA SER ASN ASN THR ALA SER ILE ALA GLN ALA ARG          
SEQRES   2 G   68  LYS LEU VAL GLU GLN LEU LYS MET GLU ALA ASN ILE ASP          
SEQRES   3 G   68  ARG ILE LYS VAL SER LYS ALA ALA ALA ASP LEU MET ALA          
SEQRES   4 G   68  TYR CYS GLU ALA HIS ALA LYS GLU ASP PRO LEU LEU THR          
SEQRES   5 G   68  PRO VAL PRO ALA SER GLU ASN PRO PHE ARG GLU LYS LYS          
SEQRES   6 G   68  PHE PHE CYS                                                  
SEQRES   1 N  138  GLN VAL GLN LEU GLN GLU SER GLY GLY GLY LEU VAL GLN          
SEQRES   2 N  138  PRO GLY GLY SER LEU ARG LEU SER CYS ALA ALA SER GLY          
SEQRES   3 N  138  PHE THR PHE SER ASN TYR LYS MET ASN TRP VAL ARG GLN          
SEQRES   4 N  138  ALA PRO GLY LYS GLY LEU GLU TRP VAL SER ASP ILE SER          
SEQRES   5 N  138  GLN SER GLY ALA SER ILE SER TYR THR GLY SER VAL LYS          
SEQRES   6 N  138  GLY ARG PHE THR ILE SER ARG ASP ASN ALA LYS ASN THR          
SEQRES   7 N  138  LEU TYR LEU GLN MET ASN SER LEU LYS PRO GLU ASP THR          
SEQRES   8 N  138  ALA VAL TYR TYR CYS ALA ARG CYS PRO ALA PRO PHE THR          
SEQRES   9 N  138  ARG ASP CYS PHE ASP VAL THR SER THR THR TYR ALA TYR          
SEQRES  10 N  138  ARG GLY GLN GLY THR GLN VAL THR VAL SER SER HIS HIS          
SEQRES  11 N  138  HIS HIS HIS HIS GLU PRO GLU ALA                              
HELIX    1 AA1 SER R   31  ALA R   53  1                                  23    
HELIX    2 AA2 LEU R  123  GLU R  127  5                                   5    
HELIX    3 AA3 PRO R  137  PHE R  169  1                                  33    
HELIX    4 AA4 CYS R  174  GLN R  211  1                                  38    
HELIX    5 AA5 HIS R  212  SER R  223  1                                  12    
HELIX    6 AA6 LEU R  224  PHE R  257  1                                  34    
HELIX    7 AA7 SER R  261  TRP R  274  1                                  14    
HELIX    8 AA8 TRP R  274  GLU R  292  1                                  19    
HELIX    9 AA9 ASN R  304  ASN R  338  1                                  35    
HELIX   10 AB1 LEU R  339  THR R  343  5                                   5    
HELIX   11 AB2 ILE R  345  THR R  355  1                                  11    
HELIX   12 AB3 LEU R  356  GLY R  361  1                                   6    
HELIX   13 AB4 GLU R  364  PHE R  369  1                                   6    
HELIX   14 AB5 LEU R  379  CYS R  403  1                                  25    
HELIX   15 AB6 ASN R  406  ARG R  421  1                                  16    
HELIX   16 AB7 ALA P    8  LYS P   34  1                                  27    
HELIX   17 AB8 GLU A   10  THR A   40  1                                  31    
HELIX   18 AB9 SER A   54  GLN A   59  1                                   6    
HELIX   19 AC1 LYS A  233  PHE A  238  5                                   6    
HELIX   20 AC2 ASN A  264  ASN A  279  1                                  16    
HELIX   21 AC3 LYS A  293  GLY A  304  1                                  12    
HELIX   22 AC4 LYS A  307  PHE A  312  1                                   6    
HELIX   23 AC5 PRO A  313  ALA A  316  5                                   4    
HELIX   24 AC6 ASP A  331  GLY A  353  1                                  23    
HELIX   25 AC7 ASN A  371  TYR A  391  1                                  21    
HELIX   26 AC8 SER B    2  CYS B   25  1                                  24    
HELIX   27 AC9 THR B   29  THR B   34  1                                   6    
HELIX   28 AD1 SER G    8  ASN G   24  1                                  17    
HELIX   29 AD2 LYS G   29  ALA G   45  1                                  17    
HELIX   30 AD3 LYS G   46  ASP G   48  5                                   3    
HELIX   31 AD4 THR N   28  TYR N   32  5                                   5    
SHEET    1 AA1 2 THR R  65  PHE R  66  0                                        
SHEET    2 AA1 2 CYS R  71  TRP R  72 -1  O  TRP R  72   N  THR R  65           
SHEET    1 AA2 2 SER R  79  SER R  84  0                                        
SHEET    2 AA2 2 HIS R  99  CYS R 104 -1  O  ARG R 102   N  VAL R  81           
SHEET    1 AA3 6 THR A 210  VAL A 214  0                                        
SHEET    2 AA3 6 VAL A 217  ASP A 223 -1  O  PHE A 219   N  PHE A 212           
SHEET    3 AA3 6 HIS A  41  LEU A  46  1  N  LEU A  43   O  HIS A 220           
SHEET    4 AA3 6 ALA A 243  ALA A 249  1  O  VAL A 247   N  LEU A  46           
SHEET    5 AA3 6 SER A 286  ASN A 292  1  O  ASN A 292   N  VAL A 248           
SHEET    6 AA3 6 CYS A 359  TYR A 360  1  O  TYR A 360   N  LEU A 289           
SHEET    1 AA4 4 THR B  47  ARG B  52  0                                        
SHEET    2 AA4 4 PHE B 335  TRP B 339 -1  O  LEU B 336   N  LEU B  51           
SHEET    3 AA4 4 VAL B 327  SER B 331 -1  N  THR B 329   O  LYS B 337           
SHEET    4 AA4 4 VAL B 315  VAL B 320 -1  N  GLY B 319   O  ALA B 328           
SHEET    1 AA5 4 ILE B  58  TRP B  63  0                                        
SHEET    2 AA5 4 LEU B  69  SER B  74 -1  O  VAL B  71   N  HIS B  62           
SHEET    3 AA5 4 LYS B  78  ASP B  83 -1  O  ILE B  80   N  SER B  72           
SHEET    4 AA5 4 LYS B  89  PRO B  94 -1  O  HIS B  91   N  ILE B  81           
SHEET    1 AA6 4 THR B 102  TYR B 105  0                                        
SHEET    2 AA6 4 TYR B 111  GLY B 115 -1  O  ALA B 113   N  ALA B 104           
SHEET    3 AA6 4 CYS B 121  ASN B 125 -1  O  TYR B 124   N  VAL B 112           
SHEET    4 AA6 4 VAL B 135  LEU B 139 -1  O  LEU B 139   N  CYS B 121           
SHEET    1 AA7 4 LEU B 146  PHE B 151  0                                        
SHEET    2 AA7 4 GLN B 156  SER B 161 -1  O  SER B 160   N  CYS B 148           
SHEET    3 AA7 4 CYS B 166  ASP B 170 -1  O  TRP B 169   N  ILE B 157           
SHEET    4 AA7 4 GLN B 175  PHE B 180 -1  O  THR B 178   N  LEU B 168           
SHEET    1 AA8 4 VAL B 187  LEU B 192  0                                        
SHEET    2 AA8 4 LEU B 198  ALA B 203 -1  O  GLY B 202   N  SER B 189           
SHEET    3 AA8 4 ALA B 208  ASP B 212 -1  O  TRP B 211   N  PHE B 199           
SHEET    4 AA8 4 CYS B 218  PHE B 222 -1  O  PHE B 222   N  ALA B 208           
SHEET    1 AA9 4 ILE B 229  PHE B 234  0                                        
SHEET    2 AA9 4 ALA B 240  SER B 245 -1  O  GLY B 244   N  ALA B 231           
SHEET    3 AA9 4 CYS B 250  ASP B 254 -1  O  PHE B 253   N  PHE B 241           
SHEET    4 AA9 4 GLU B 260  TYR B 264 -1  O  TYR B 264   N  CYS B 250           
SHEET    1 AB1 4 ILE B 273  PHE B 278  0                                        
SHEET    2 AB1 4 LEU B 284  TYR B 289 -1  O  LEU B 286   N  SER B 277           
SHEET    3 AB1 4 CYS B 294  ASP B 298 -1  O  TRP B 297   N  LEU B 285           
SHEET    4 AB1 4 ARG B 304  LEU B 308 -1  O  GLY B 306   N  VAL B 296           
SHEET    1 AB2 4 GLN N   3  GLU N   6  0                                        
SHEET    2 AB2 4 LEU N  18  SER N  25 -1  O  SER N  25   N  GLN N   3           
SHEET    3 AB2 4 THR N  78  MET N  83 -1  O  LEU N  79   N  CYS N  22           
SHEET    4 AB2 4 PHE N  68  ASP N  73 -1  N  ASP N  73   O  THR N  78           
SHEET    1 AB3 6 GLY N  10  VAL N  12  0                                        
SHEET    2 AB3 6 THR N 122  VAL N 126  1  O  THR N 125   N  GLY N  10           
SHEET    3 AB3 6 ALA N  92  ARG N  98 -1  N  TYR N  94   O  THR N 122           
SHEET    4 AB3 6 MET N  34  GLN N  39 -1  N  GLN N  39   O  VAL N  93           
SHEET    5 AB3 6 LEU N  45  ILE N  51 -1  O  ILE N  51   N  MET N  34           
SHEET    6 AB3 6 ILE N  58  TYR N  60 -1  O  SER N  59   N  ASP N  50           
SSBOND   1 CYS R   46    CYS R   71                          1555   1555  2.95  
SSBOND   2 CYS R   62    CYS R  104                          1555   1555  2.02  
SSBOND   3 CYS R   85    CYS R  126                          1555   1555  2.03  
SSBOND   4 CYS R  226    CYS R  296                          1555   1555  2.04  
SSBOND   5 CYS N   22    CYS N   96                          1555   1555  2.03  
SSBOND   6 CYS N   99    CYS N  107                          1555   1555  2.03  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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