GenomeNet

Database: PDB
Entry: 5WI4
LinkDB: 5WI4
Original site: 5WI4 
HEADER    MOTOR PROTEIN                           18-JUL-17   5WI4              
TITLE     CRYSTAL STRUCTURE OF DYNLT1/TCTEX-1 IN COMPLEX WITH ARHGEF2           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DYNEIN LIGHT CHAIN TCTEX-TYPE 1,RHO GUANINE NUCLEOTIDE     
COMPND   3 EXCHANGE FACTOR 2;                                                   
COMPND   4 CHAIN: A, B, C;                                                      
COMPND   5 FRAGMENT: UNP RESIDUE 1-113; UNP RESIDUES 139-164;                   
COMPND   6 SYNONYM: ACTIVATOR OF G-PROTEIN SIGNALING 2,AGS2,T-COMPLEX TESTIS-   
COMPND   7 SPECIFIC PROTEIN 1,TCTEX-1,GUANINE NUCLEOTIDE EXCHANGE FACTOR H1,GEF-
COMPND   8 H1,LBC'S FIRST COUSIN,LYMPHOID BLAST CRISIS-LIKE 1,ONCOGENE LFC,     
COMPND   9 RHOBIN;                                                              
COMPND  10 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: MOUSE;                                              
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 GENE: DYNLT1, TCTEL1, TCTEX-1, TCTEX1, ARHGEF2, KIAA0651, LBCL1,     
SOURCE   6 LFC;                                                                 
SOURCE   7 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   8 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   9 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE  10 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  11 EXPRESSION_SYSTEM_PLASMID: PGEX4T-1                                  
KEYWDS    DYNEIN LIGHT CHAIN, RHO GEF, MOTOR PROTEIN, CARGO TRANSPORT           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.BALAN,N.ISHIYAMA,C.B.MARSHALL,M.IKURA                               
REVDAT   2   04-OCT-23 5WI4    1       REMARK                                   
REVDAT   1   15-NOV-17 5WI4    0                                                
JRNL        AUTH   M.J.SANDI,C.B.MARSHALL,M.BALAN,E.COYAUD,M.ZHOU,D.M.MONSON,   
JRNL        AUTH 2 N.ISHIYAMA,A.A.CHANDRAKUMAR,J.LA ROSE,A.L.COUZENS,           
JRNL        AUTH 3 A.C.GINGRAS,B.RAUGHT,W.XU,M.IKURA,D.K.MORRISON,R.ROTTAPEL    
JRNL        TITL   MARK3-MEDIATED PHOSPHORYLATION OF ARHGEF2 COUPLES            
JRNL        TITL 2 MICROTUBULES TO THE ACTIN CYTOSKELETON TO ESTABLISH CELL     
JRNL        TITL 3 POLARITY.                                                    
JRNL        REF    SCI SIGNAL                    V.  10       2017              
JRNL        REFN                   ESSN 1937-9145                               
JRNL        PMID   29089450                                                     
JRNL        DOI    10.1126/SCISIGNAL.AAN3286                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.8.2_1309                                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 36.83                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 0.130                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 27423                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.176                           
REMARK   3   R VALUE            (WORKING SET) : 0.174                           
REMARK   3   FREE R VALUE                     : 0.210                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 7.330                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2010                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 36.8373 -  4.8137    0.93     1962   152  0.1693 0.2058        
REMARK   3     2  4.8137 -  3.8220    0.93     1879   143  0.1283 0.1783        
REMARK   3     3  3.8220 -  3.3393    0.93     1880   144  0.1447 0.1632        
REMARK   3     4  3.3393 -  3.0341    0.93     1857   145  0.1647 0.1850        
REMARK   3     5  3.0341 -  2.8167    0.93     1829   143  0.1868 0.2336        
REMARK   3     6  2.8167 -  2.6507    0.92     1821   143  0.1979 0.2628        
REMARK   3     7  2.6507 -  2.5180    0.92     1822   140  0.1938 0.2370        
REMARK   3     8  2.5180 -  2.4084    0.91     1787   145  0.2017 0.2313        
REMARK   3     9  2.4084 -  2.3157    0.91     1795   141  0.2052 0.2531        
REMARK   3    10  2.3157 -  2.2358    0.91     1780   136  0.2003 0.2322        
REMARK   3    11  2.2358 -  2.1659    0.90     1785   139  0.1988 0.2057        
REMARK   3    12  2.1659 -  2.1040    0.89     1766   143  0.2042 0.2196        
REMARK   3    13  2.1040 -  2.0486    0.89     1741   140  0.2066 0.2576        
REMARK   3    14  2.0486 -  2.0000    0.88     1703   137  0.2157 0.2334        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : NULL             
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 27.870           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007           2874                                  
REMARK   3   ANGLE     :  1.043           3897                                  
REMARK   3   CHIRALITY :  0.068            473                                  
REMARK   3   PLANARITY :  0.003            474                                  
REMARK   3   DIHEDRAL  : 13.688            983                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5WI4 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 19-JUL-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000229035.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 15-AUG-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7                                  
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 19-BM                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9791                             
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 27423                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.000                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 10.80                              
REMARK 200  R MERGE                    (I) : 0.07800                            
REMARK 200  R SYM                      (I) : 0.07800                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 31.4920                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.03                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 9.20                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.34800                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 6.578                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: COOT (0.7)                                            
REMARK 200 STARTING MODEL: 1YGT                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 42.99                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.16                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 2.33M AMMONIUM SULPHATE, 0.25MM SODIUM   
REMARK 280  MALONATE, 7.2MM CALCIUM CHLORIDE, PH 7, VAPOR DIFFUSION, HANGING    
REMARK 280  DROP, TEMPERATURE 295K                                              
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 32 2 1                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   Y,X,-Z                                                  
REMARK 290       5555   X-Y,-Y,-Z+1/3                                           
REMARK 290       6555   -X,-X+Y,-Z+2/3                                          
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       55.38600            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       27.69300            
REMARK 290   SMTRY1   4 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   5  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       27.69300            
REMARK 290   SMTRY1   6 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       55.38600            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 4480 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 18330 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -84.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH C 228  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    -1                                                      
REMARK 465     SER A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     GLU A     2                                                      
REMARK 465     ASP A     3                                                      
REMARK 465     PHE A     4                                                      
REMARK 465     GLY A    74                                                      
REMARK 465     ALA A    75                                                      
REMARK 465     GLY A   114                                                      
REMARK 465     GLY A   115                                                      
REMARK 465     SER A   116                                                      
REMARK 465     ARG A   117                                                      
REMARK 465     GLY A   136                                                      
REMARK 465     HIS A   137                                                      
REMARK 465     PHE A   138                                                      
REMARK 465     ASN A   139                                                      
REMARK 465     ASP A   140                                                      
REMARK 465     GLU A   141                                                      
REMARK 465     SER A   142                                                      
REMARK 465     PRO A   143                                                      
REMARK 465     GLY B    -1                                                      
REMARK 465     SER B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     GLU B     2                                                      
REMARK 465     ASP B     3                                                      
REMARK 465     PHE B     4                                                      
REMARK 465     ASN B    73                                                      
REMARK 465     GLY B    74                                                      
REMARK 465     ALA B    75                                                      
REMARK 465     GLY B   115                                                      
REMARK 465     SER B   116                                                      
REMARK 465     ARG B   117                                                      
REMARK 465     GLY B   136                                                      
REMARK 465     HIS B   137                                                      
REMARK 465     PHE B   138                                                      
REMARK 465     ASN B   139                                                      
REMARK 465     ASP B   140                                                      
REMARK 465     GLU B   141                                                      
REMARK 465     SER B   142                                                      
REMARK 465     PRO B   143                                                      
REMARK 465     GLY C    -1                                                      
REMARK 465     SER C     0                                                      
REMARK 465     MET C     1                                                      
REMARK 465     GLU C     2                                                      
REMARK 465     ASP C     3                                                      
REMARK 465     PHE C     4                                                      
REMARK 465     GLY C    74                                                      
REMARK 465     ALA C    75                                                      
REMARK 465     GLY C   114                                                      
REMARK 465     GLY C   115                                                      
REMARK 465     SER C   116                                                      
REMARK 465     ALA C   135                                                      
REMARK 465     GLY C   136                                                      
REMARK 465     HIS C   137                                                      
REMARK 465     PHE C   138                                                      
REMARK 465     ASN C   139                                                      
REMARK 465     ASP C   140                                                      
REMARK 465     GLU C   141                                                      
REMARK 465     SER C   142                                                      
REMARK 465     PRO C   143                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLN A   5    CG   CD   OE1  NE2                                  
REMARK 470     ASN A  73    CG   OD1  ND2                                       
REMARK 470     LYS A 100    CG   CD   CE   NZ                                   
REMARK 470     ARG A 118    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLN B   5    CG   CD   OE1  NE2                                  
REMARK 470     ARG B 118    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLN C   5    CG   CD   OE1  NE2                                  
REMARK 470     ASN C  73    CG   OD1  ND2                                       
REMARK 470     LYS C 100    CG   CD   CE   NZ                                   
REMARK 470     ILE C 113    CG1  CG2  CD1                                       
REMARK 470     ARG C 117    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE C 134    CG1  CG2  CD1                                       
REMARK 480                                                                      
REMARK 480 ZERO OCCUPANCY ATOM                                                  
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO                  
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS                
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;              
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):         
REMARK 480   M RES C SSEQI ATOMS                                                
REMARK 480     LYS A   22   CD   CE   NZ                                        
REMARK 480     GLN A   41   CD   OE1  NE2                                       
REMARK 480     GLU A   48   CG   CD   OE1  OE2                                  
REMARK 480     GLU A   98   CD   OE1  OE2                                       
REMARK 480     SER A  124   OG                                                  
REMARK 480     LYS A  127   CE   NZ                                             
REMARK 480     LYS B   22   CD   CE   NZ                                        
REMARK 480     ASN B   40   OD1  ND2                                            
REMARK 480     GLN B   41   CG   CD   OE1  NE2                                  
REMARK 480     GLU B   48   CG   CD   OE1  OE2                                  
REMARK 480     LYS B  100   CG   CD   CE   NZ                                   
REMARK 480     SER C   37   CA                                                  
REMARK 480     GLN C   41   CG   CD   OE1  NE2                                  
REMARK 480     GLU C   48   CG   CD   OE1  OE2                                  
REMARK 480     GLN C   71   CG                                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A  99     -161.49   -127.02                                   
REMARK 500    ASN C  99     -153.92   -126.92                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 202                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 203                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 B 201                 
DBREF  5WI4 A    1   113  UNP    P51807   DYLT1_MOUSE      1    113             
DBREF  5WI4 A  118   143  UNP    Q60875   ARHG2_MOUSE    139    164             
DBREF  5WI4 B    1   113  UNP    P51807   DYLT1_MOUSE      1    113             
DBREF  5WI4 B  118   143  UNP    Q60875   ARHG2_MOUSE    139    164             
DBREF  5WI4 C    1   113  UNP    P51807   DYLT1_MOUSE      1    113             
DBREF  5WI4 C  118   143  UNP    Q60875   ARHG2_MOUSE    139    164             
SEQADV 5WI4 GLY A   -1  UNP  P51807              EXPRESSION TAG                 
SEQADV 5WI4 SER A    0  UNP  P51807              EXPRESSION TAG                 
SEQADV 5WI4 GLY A  114  UNP  P51807              LINKER                         
SEQADV 5WI4 GLY A  115  UNP  P51807              LINKER                         
SEQADV 5WI4 SER A  116  UNP  P51807              LINKER                         
SEQADV 5WI4 ARG A  117  UNP  P51807              LINKER                         
SEQADV 5WI4 GLY B   -1  UNP  P51807              EXPRESSION TAG                 
SEQADV 5WI4 SER B    0  UNP  P51807              EXPRESSION TAG                 
SEQADV 5WI4 GLY B  114  UNP  P51807              LINKER                         
SEQADV 5WI4 GLY B  115  UNP  P51807              LINKER                         
SEQADV 5WI4 SER B  116  UNP  P51807              LINKER                         
SEQADV 5WI4 ARG B  117  UNP  P51807              LINKER                         
SEQADV 5WI4 GLY C   -1  UNP  P51807              EXPRESSION TAG                 
SEQADV 5WI4 SER C    0  UNP  P51807              EXPRESSION TAG                 
SEQADV 5WI4 GLY C  114  UNP  P51807              LINKER                         
SEQADV 5WI4 GLY C  115  UNP  P51807              LINKER                         
SEQADV 5WI4 SER C  116  UNP  P51807              LINKER                         
SEQADV 5WI4 ARG C  117  UNP  P51807              LINKER                         
SEQRES   1 A  145  GLY SER MET GLU ASP PHE GLN ALA SER GLU GLU THR ALA          
SEQRES   2 A  145  PHE VAL VAL ASP GLU VAL SER SER ILE VAL LYS GLU ALA          
SEQRES   3 A  145  ILE GLU SER ALA ILE GLY GLY ASN ALA TYR GLN HIS SER          
SEQRES   4 A  145  LYS VAL ASN GLN TRP THR THR ASN VAL LEU GLU GLN THR          
SEQRES   5 A  145  LEU SER GLN LEU THR LYS LEU GLY ARG PRO PHE LYS TYR          
SEQRES   6 A  145  ILE VAL THR CYS VAL ILE MET GLN LYS ASN GLY ALA GLY          
SEQRES   7 A  145  LEU HIS SER ALA SER SER CYS PHE TRP ASP SER SER THR          
SEQRES   8 A  145  ASP GLY SER CYS THR VAL ARG TRP GLU ASN LYS THR MET          
SEQRES   9 A  145  TYR CYS ILE VAL SER THR PHE GLY LEU SER ILE GLY GLY          
SEQRES  10 A  145  SER ARG ARG GLY LEU SER SER LEU SER LEU ALA LYS SER          
SEQRES  11 A  145  VAL SER THR THR ASN ILE ALA GLY HIS PHE ASN ASP GLU          
SEQRES  12 A  145  SER PRO                                                      
SEQRES   1 B  145  GLY SER MET GLU ASP PHE GLN ALA SER GLU GLU THR ALA          
SEQRES   2 B  145  PHE VAL VAL ASP GLU VAL SER SER ILE VAL LYS GLU ALA          
SEQRES   3 B  145  ILE GLU SER ALA ILE GLY GLY ASN ALA TYR GLN HIS SER          
SEQRES   4 B  145  LYS VAL ASN GLN TRP THR THR ASN VAL LEU GLU GLN THR          
SEQRES   5 B  145  LEU SER GLN LEU THR LYS LEU GLY ARG PRO PHE LYS TYR          
SEQRES   6 B  145  ILE VAL THR CYS VAL ILE MET GLN LYS ASN GLY ALA GLY          
SEQRES   7 B  145  LEU HIS SER ALA SER SER CYS PHE TRP ASP SER SER THR          
SEQRES   8 B  145  ASP GLY SER CYS THR VAL ARG TRP GLU ASN LYS THR MET          
SEQRES   9 B  145  TYR CYS ILE VAL SER THR PHE GLY LEU SER ILE GLY GLY          
SEQRES  10 B  145  SER ARG ARG GLY LEU SER SER LEU SER LEU ALA LYS SER          
SEQRES  11 B  145  VAL SER THR THR ASN ILE ALA GLY HIS PHE ASN ASP GLU          
SEQRES  12 B  145  SER PRO                                                      
SEQRES   1 C  145  GLY SER MET GLU ASP PHE GLN ALA SER GLU GLU THR ALA          
SEQRES   2 C  145  PHE VAL VAL ASP GLU VAL SER SER ILE VAL LYS GLU ALA          
SEQRES   3 C  145  ILE GLU SER ALA ILE GLY GLY ASN ALA TYR GLN HIS SER          
SEQRES   4 C  145  LYS VAL ASN GLN TRP THR THR ASN VAL LEU GLU GLN THR          
SEQRES   5 C  145  LEU SER GLN LEU THR LYS LEU GLY ARG PRO PHE LYS TYR          
SEQRES   6 C  145  ILE VAL THR CYS VAL ILE MET GLN LYS ASN GLY ALA GLY          
SEQRES   7 C  145  LEU HIS SER ALA SER SER CYS PHE TRP ASP SER SER THR          
SEQRES   8 C  145  ASP GLY SER CYS THR VAL ARG TRP GLU ASN LYS THR MET          
SEQRES   9 C  145  TYR CYS ILE VAL SER THR PHE GLY LEU SER ILE GLY GLY          
SEQRES  10 C  145  SER ARG ARG GLY LEU SER SER LEU SER LEU ALA LYS SER          
SEQRES  11 C  145  VAL SER THR THR ASN ILE ALA GLY HIS PHE ASN ASP GLU          
SEQRES  12 C  145  SER PRO                                                      
HET    SO4  A 201       5                                                       
HET    SO4  A 202       5                                                       
HET    SO4  A 203       5                                                       
HET    SO4  B 201       5                                                       
HETNAM     SO4 SULFATE ION                                                      
FORMUL   4  SO4    4(O4 S 2-)                                                   
FORMUL   8  HOH   *178(H2 O)                                                    
HELIX    1 AA1 VAL A   13  GLY A   30  1                                  18    
HELIX    2 AA2 GLN A   35  SER A   37  5                                   3    
HELIX    3 AA3 LYS A   38  LYS A   56  1                                  19    
HELIX    4 AA4 ARG A  118  LEU A  123  5                                   6    
HELIX    5 AA5 VAL B   13  GLY B   30  1                                  18    
HELIX    6 AA6 LYS B   38  LEU B   57  1                                  20    
HELIX    7 AA7 ARG B  118  LEU B  123  5                                   6    
HELIX    8 AA8 VAL C   13  GLY C   30  1                                  18    
HELIX    9 AA9 GLN C   35  SER C   37  5                                   3    
HELIX   10 AB1 LYS C   38  LEU C   57  1                                  20    
HELIX   11 AB2 ARG C  118  LEU C  123  5                                   6    
SHEET    1 AA1 5 GLY A  91  GLU A  98  0                                        
SHEET    2 AA1 5 MET A 102  SER A 112 -1  O  CYS A 104   N  TRP A  97           
SHEET    3 AA1 5 PHE A  61  GLN A  71 -1  N  ILE A  64   O  PHE A 109           
SHEET    4 AA1 5 LEU B  77  TRP B  85 -1  O  SER B  82   N  VAL A  65           
SHEET    5 AA1 5 SER B 124  ILE B 134 -1  O  ILE B 134   N  LEU B  77           
SHEET    1 AA2 3 SER A 124  ALA A 126  0                                        
SHEET    2 AA2 3 LEU A  77  TRP A  85 -1  N  CYS A  83   O  ALA A 126           
SHEET    3 AA2 3 THR A 132  ILE A 134 -1  O  ILE A 134   N  LEU A  77           
SHEET    1 AA3 5 SER A 124  ALA A 126  0                                        
SHEET    2 AA3 5 LEU A  77  TRP A  85 -1  N  CYS A  83   O  ALA A 126           
SHEET    3 AA3 5 PHE B  61  GLN B  71 -1  O  VAL B  65   N  SER A  82           
SHEET    4 AA3 5 MET B 102  SER B 112 -1  O  ILE B 105   N  VAL B  68           
SHEET    5 AA3 5 GLY B  91  GLU B  98 -1  N  TRP B  97   O  CYS B 104           
SHEET    1 AA4 3 PHE C  61  GLN C  71  0                                        
SHEET    2 AA4 3 MET C 102  SER C 112 -1  O  ILE C 105   N  VAL C  68           
SHEET    3 AA4 3 GLY C  91  GLU C  98 -1  N  CYS C  93   O  THR C 108           
SHEET    1 AA5 2 LEU C  77  SER C  79  0                                        
SHEET    2 AA5 2 THR C 132  ILE C 134 -1  O  ILE C 134   N  LEU C  77           
SHEET    1 AA6 2 CYS C  83  TRP C  85  0                                        
SHEET    2 AA6 2 SER C 124  ALA C 126 -1  O  SER C 124   N  TRP C  85           
SITE     1 AC1  6 ASN A  32  LYS A  38  TRP A  42  SER A 121                    
SITE     2 AC1  6 HOH A 329  HOH A 335                                          
SITE     1 AC2  5 TYR A  34  HIS A  36  GLN A  71  HIS B  78                    
SITE     2 AC2  5 ALA B 135                                                     
SITE     1 AC3  6 GLY A  76  HIS A  78  ALA A 135  TYR B  34                    
SITE     2 AC3  6 HIS B  36  GLN B  71                                          
SITE     1 AC4  4 ASN B  32  LYS B  38  TRP B  42  SER C 121                    
CRYST1   91.967   91.967   83.079  90.00  90.00 120.00 P 32 2 1     18          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.010873  0.006278  0.000000        0.00000                         
SCALE2      0.000000  0.012556  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.012037        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system