HEADER MEMBRANE PROTEIN 07-AUG-17 5WPT
TITLE CRYO-EM STRUCTURE OF MAMMALIAN ENDOLYSOSOMAL TRPML1 CHANNEL IN
TITLE 2 NANODISCS IN CLOSED II CONFORMATION AT 3.75 ANGSTROM RESOLUTION
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: MUCOLIPIN-1;
COMPND 3 CHAIN: A, B, C, D;
COMPND 4 SYNONYM: TRPML1, MUCOLIPIDIN;
COMPND 5 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;
SOURCE 3 ORGANISM_COMMON: MOUSE;
SOURCE 4 ORGANISM_TAXID: 10090;
SOURCE 5 GENE: MCOLN1;
SOURCE 6 EXPRESSION_SYSTEM: HOMO SAPIENS;
SOURCE 7 EXPRESSION_SYSTEM_COMMON: HUMAN;
SOURCE 8 EXPRESSION_SYSTEM_TAXID: 9606;
SOURCE 9 EXPRESSION_SYSTEM_CELL_LINE: HEK293F;
SOURCE 10 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 11 EXPRESSION_SYSTEM_PLASMID: PEZT
KEYWDS ION CHANNEL, MEMBRANE PROTEIN
EXPDTA ELECTRON MICROSCOPY
AUTHOR Q.CHEN,J.SHE,J.GUO,X.BAI,Y.JIANG
REVDAT 6 05-APR-23 5WPT 1 REMARK HETSYN
REVDAT 5 29-JUL-20 5WPT 1 COMPND REMARK HETNAM LINK
REVDAT 5 2 1 SITE ATOM
REVDAT 4 20-NOV-19 5WPT 1 REMARK
REVDAT 3 24-APR-19 5WPT 1 REMARK
REVDAT 2 25-OCT-17 5WPT 1 JRNL
REVDAT 1 18-OCT-17 5WPT 0
JRNL AUTH Q.CHEN,J.SHE,W.ZENG,J.GUO,H.XU,X.C.BAI,Y.JIANG
JRNL TITL STRUCTURE OF MAMMALIAN ENDOLYSOSOMAL TRPML1 CHANNEL IN
JRNL TITL 2 NANODISCS.
JRNL REF NATURE V. 550 415 2017
JRNL REFN ISSN 0028-0836
JRNL PMID 29019981
JRNL DOI 10.1038/NATURE24035
REMARK 2
REMARK 2 RESOLUTION. 3.75 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 SOFTWARE PACKAGES : RELION, EPU, GCTF, RELION, RELION,
REMARK 3 RELION, RELION
REMARK 3 RECONSTRUCTION SCHEMA : BACK PROJECTION
REMARK 3
REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT
REMARK 3 PDB ENTRY : NULL
REMARK 3 REFINEMENT SPACE : NULL
REMARK 3 REFINEMENT PROTOCOL : NULL
REMARK 3 REFINEMENT TARGET : NULL
REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL
REMARK 3
REMARK 3 FITTING PROCEDURE : NULL
REMARK 3
REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS
REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 3.750
REMARK 3 NUMBER OF PARTICLES : 11000
REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE
REMARK 3 CORRECTION
REMARK 3
REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL
REMARK 3
REMARK 3 OTHER DETAILS: NULL
REMARK 4
REMARK 4 5WPT COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 08-AUG-17.
REMARK 100 THE DEPOSITION ID IS D_1000229422.
REMARK 245
REMARK 245 EXPERIMENTAL DETAILS
REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE
REMARK 245 SPECIMEN TYPE : NULL
REMARK 245
REMARK 245 ELECTRON MICROSCOPE SAMPLE
REMARK 245 SAMPLE TYPE : PARTICLE
REMARK 245 PARTICLE TYPE : 2D CRYSTAL
REMARK 245 NAME OF SAMPLE : HOMOTETRAMER OF MOUSE TRPML1
REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : 1.30
REMARK 245 SAMPLE SUPPORT DETAILS : NULL
REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL
REMARK 245 SAMPLE BUFFER : NULL
REMARK 245 PH : 8.00
REMARK 245 SAMPLE DETAILS : NULL
REMARK 245
REMARK 245 DATA ACQUISITION
REMARK 245 DATE OF EXPERIMENT : NULL
REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : 3000
REMARK 245 TEMPERATURE (KELVIN) : NULL
REMARK 245 MICROSCOPE MODEL : FEI TITAN KRIOS
REMARK 245 DETECTOR TYPE : GATAN K2 SUMMIT (4K X 4K)
REMARK 245 MINIMUM DEFOCUS (NM) : 1200.00
REMARK 245 MAXIMUM DEFOCUS (NM) : 3000.00
REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 NOMINAL CS : 2.70
REMARK 245 IMAGING MODE : BRIGHT FIELD
REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 5000.00
REMARK 245 ILLUMINATION MODE : FLOOD BEAM
REMARK 245 NOMINAL MAGNIFICATION : 46730
REMARK 245 CALIBRATED MAGNIFICATION : NULL
REMARK 245 SOURCE : FIELD EMISSION GUN
REMARK 245 ACCELERATION VOLTAGE (KV) : 300
REMARK 245 IMAGING DETAILS : NULL
REMARK 247
REMARK 247 ELECTRON MICROSCOPY
REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON
REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE
REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES
REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION
REMARK 247 OF THE STRUCTURE FACTORS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F, G, H
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 MET A 1
REMARK 465 ALA A 2
REMARK 465 THR A 3
REMARK 465 PRO A 4
REMARK 465 ALA A 5
REMARK 465 GLY A 6
REMARK 465 ARG A 7
REMARK 465 ARG A 8
REMARK 465 ALA A 9
REMARK 465 SER A 10
REMARK 465 GLU A 11
REMARK 465 THR A 12
REMARK 465 GLU A 13
REMARK 465 ARG A 14
REMARK 465 LEU A 15
REMARK 465 LEU A 16
REMARK 465 THR A 17
REMARK 465 PRO A 18
REMARK 465 ASN A 19
REMARK 465 PRO A 20
REMARK 465 GLY A 21
REMARK 465 TYR A 22
REMARK 465 GLY A 23
REMARK 465 THR A 24
REMARK 465 GLN A 25
REMARK 465 VAL A 26
REMARK 465 GLY A 27
REMARK 465 THR A 28
REMARK 465 SER A 29
REMARK 465 PRO A 30
REMARK 465 ALA A 31
REMARK 465 PRO A 32
REMARK 465 THR A 33
REMARK 465 THR A 34
REMARK 465 PRO A 35
REMARK 465 THR A 36
REMARK 465 GLU A 37
REMARK 465 GLY A 152
REMARK 465 GLY A 153
REMARK 465 GLY A 154
REMARK 465 GLY A 155
REMARK 465 PRO A 156
REMARK 465 TRP A 157
REMARK 465 ALA A 158
REMARK 465 ASN A 159
REMARK 465 GLY A 160
REMARK 465 SER A 161
REMARK 465 ASP A 199
REMARK 465 ARG A 200
REMARK 465 PRO A 201
REMARK 465 PRO A 202
REMARK 465 ASP A 203
REMARK 465 ILE A 204
REMARK 465 PRO A 205
REMARK 465 SER A 206
REMARK 465 GLU A 207
REMARK 465 ASP A 208
REMARK 465 LEU A 209
REMARK 465 ASP A 210
REMARK 465 PHE A 211
REMARK 465 LEU A 212
REMARK 465 ASP A 213
REMARK 465 GLY A 214
REMARK 465 HIS A 286
REMARK 465 PRO A 287
REMARK 465 SER A 288
REMARK 465 VAL A 289
REMARK 465 SER A 290
REMARK 465 ARG A 291
REMARK 465 HIS A 292
REMARK 465 GLY A 293
REMARK 465 ASP A 294
REMARK 465 ASN A 295
REMARK 465 GLY A 528
REMARK 465 GLY A 529
REMARK 465 THR A 530
REMARK 465 GLY A 531
REMARK 465 THR A 532
REMARK 465 GLU A 533
REMARK 465 LYS A 534
REMARK 465 SER A 535
REMARK 465 GLU A 536
REMARK 465 LEU A 537
REMARK 465 GLN A 538
REMARK 465 ALA A 539
REMARK 465 TYR A 540
REMARK 465 ILE A 541
REMARK 465 GLU A 542
REMARK 465 GLN A 543
REMARK 465 CYS A 544
REMARK 465 GLN A 545
REMARK 465 ASP A 546
REMARK 465 SER A 547
REMARK 465 PRO A 548
REMARK 465 THR A 549
REMARK 465 SER A 550
REMARK 465 GLY A 551
REMARK 465 LYS A 552
REMARK 465 PHE A 553
REMARK 465 ARG A 554
REMARK 465 ARG A 555
REMARK 465 GLY A 556
REMARK 465 SER A 557
REMARK 465 GLY A 558
REMARK 465 SER A 559
REMARK 465 ALA A 560
REMARK 465 CYS A 561
REMARK 465 SER A 562
REMARK 465 LEU A 563
REMARK 465 PHE A 564
REMARK 465 CYS A 565
REMARK 465 CYS A 566
REMARK 465 CYS A 567
REMARK 465 GLY A 568
REMARK 465 ARG A 569
REMARK 465 ASP A 570
REMARK 465 SER A 571
REMARK 465 PRO A 572
REMARK 465 GLU A 573
REMARK 465 ASP A 574
REMARK 465 HIS A 575
REMARK 465 SER A 576
REMARK 465 LEU A 577
REMARK 465 LEU A 578
REMARK 465 VAL A 579
REMARK 465 ASN A 580
REMARK 465 VAL A 581
REMARK 465 ASP A 582
REMARK 465 GLY A 583
REMARK 465 GLY A 584
REMARK 465 SER A 585
REMARK 465 SER A 586
REMARK 465 GLY A 587
REMARK 465 GLY A 588
REMARK 465 LEU A 589
REMARK 465 VAL A 590
REMARK 465 PRO A 591
REMARK 465 ARG A 592
REMARK 465 MET B 1
REMARK 465 ALA B 2
REMARK 465 THR B 3
REMARK 465 PRO B 4
REMARK 465 ALA B 5
REMARK 465 GLY B 6
REMARK 465 ARG B 7
REMARK 465 ARG B 8
REMARK 465 ALA B 9
REMARK 465 SER B 10
REMARK 465 GLU B 11
REMARK 465 THR B 12
REMARK 465 GLU B 13
REMARK 465 ARG B 14
REMARK 465 LEU B 15
REMARK 465 LEU B 16
REMARK 465 THR B 17
REMARK 465 PRO B 18
REMARK 465 ASN B 19
REMARK 465 PRO B 20
REMARK 465 GLY B 21
REMARK 465 TYR B 22
REMARK 465 GLY B 23
REMARK 465 THR B 24
REMARK 465 GLN B 25
REMARK 465 VAL B 26
REMARK 465 GLY B 27
REMARK 465 THR B 28
REMARK 465 SER B 29
REMARK 465 PRO B 30
REMARK 465 ALA B 31
REMARK 465 PRO B 32
REMARK 465 THR B 33
REMARK 465 THR B 34
REMARK 465 PRO B 35
REMARK 465 THR B 36
REMARK 465 GLU B 37
REMARK 465 GLY B 152
REMARK 465 GLY B 153
REMARK 465 GLY B 154
REMARK 465 GLY B 155
REMARK 465 PRO B 156
REMARK 465 TRP B 157
REMARK 465 ALA B 158
REMARK 465 ASN B 159
REMARK 465 GLY B 160
REMARK 465 SER B 161
REMARK 465 ASP B 199
REMARK 465 ARG B 200
REMARK 465 PRO B 201
REMARK 465 PRO B 202
REMARK 465 ASP B 203
REMARK 465 ILE B 204
REMARK 465 PRO B 205
REMARK 465 SER B 206
REMARK 465 GLU B 207
REMARK 465 ASP B 208
REMARK 465 LEU B 209
REMARK 465 ASP B 210
REMARK 465 PHE B 211
REMARK 465 LEU B 212
REMARK 465 ASP B 213
REMARK 465 GLY B 214
REMARK 465 HIS B 286
REMARK 465 PRO B 287
REMARK 465 SER B 288
REMARK 465 VAL B 289
REMARK 465 SER B 290
REMARK 465 ARG B 291
REMARK 465 HIS B 292
REMARK 465 GLY B 293
REMARK 465 ASP B 294
REMARK 465 ASN B 295
REMARK 465 GLY B 528
REMARK 465 GLY B 529
REMARK 465 THR B 530
REMARK 465 GLY B 531
REMARK 465 THR B 532
REMARK 465 GLU B 533
REMARK 465 LYS B 534
REMARK 465 SER B 535
REMARK 465 GLU B 536
REMARK 465 LEU B 537
REMARK 465 GLN B 538
REMARK 465 ALA B 539
REMARK 465 TYR B 540
REMARK 465 ILE B 541
REMARK 465 GLU B 542
REMARK 465 GLN B 543
REMARK 465 CYS B 544
REMARK 465 GLN B 545
REMARK 465 ASP B 546
REMARK 465 SER B 547
REMARK 465 PRO B 548
REMARK 465 THR B 549
REMARK 465 SER B 550
REMARK 465 GLY B 551
REMARK 465 LYS B 552
REMARK 465 PHE B 553
REMARK 465 ARG B 554
REMARK 465 ARG B 555
REMARK 465 GLY B 556
REMARK 465 SER B 557
REMARK 465 GLY B 558
REMARK 465 SER B 559
REMARK 465 ALA B 560
REMARK 465 CYS B 561
REMARK 465 SER B 562
REMARK 465 LEU B 563
REMARK 465 PHE B 564
REMARK 465 CYS B 565
REMARK 465 CYS B 566
REMARK 465 CYS B 567
REMARK 465 GLY B 568
REMARK 465 ARG B 569
REMARK 465 ASP B 570
REMARK 465 SER B 571
REMARK 465 PRO B 572
REMARK 465 GLU B 573
REMARK 465 ASP B 574
REMARK 465 HIS B 575
REMARK 465 SER B 576
REMARK 465 LEU B 577
REMARK 465 LEU B 578
REMARK 465 VAL B 579
REMARK 465 ASN B 580
REMARK 465 VAL B 581
REMARK 465 ASP B 582
REMARK 465 GLY B 583
REMARK 465 GLY B 584
REMARK 465 SER B 585
REMARK 465 SER B 586
REMARK 465 GLY B 587
REMARK 465 GLY B 588
REMARK 465 LEU B 589
REMARK 465 VAL B 590
REMARK 465 PRO B 591
REMARK 465 ARG B 592
REMARK 465 MET C 1
REMARK 465 ALA C 2
REMARK 465 THR C 3
REMARK 465 PRO C 4
REMARK 465 ALA C 5
REMARK 465 GLY C 6
REMARK 465 ARG C 7
REMARK 465 ARG C 8
REMARK 465 ALA C 9
REMARK 465 SER C 10
REMARK 465 GLU C 11
REMARK 465 THR C 12
REMARK 465 GLU C 13
REMARK 465 ARG C 14
REMARK 465 LEU C 15
REMARK 465 LEU C 16
REMARK 465 THR C 17
REMARK 465 PRO C 18
REMARK 465 ASN C 19
REMARK 465 PRO C 20
REMARK 465 GLY C 21
REMARK 465 TYR C 22
REMARK 465 GLY C 23
REMARK 465 THR C 24
REMARK 465 GLN C 25
REMARK 465 VAL C 26
REMARK 465 GLY C 27
REMARK 465 THR C 28
REMARK 465 SER C 29
REMARK 465 PRO C 30
REMARK 465 ALA C 31
REMARK 465 PRO C 32
REMARK 465 THR C 33
REMARK 465 THR C 34
REMARK 465 PRO C 35
REMARK 465 THR C 36
REMARK 465 GLU C 37
REMARK 465 GLY C 152
REMARK 465 GLY C 153
REMARK 465 GLY C 154
REMARK 465 GLY C 155
REMARK 465 PRO C 156
REMARK 465 TRP C 157
REMARK 465 ALA C 158
REMARK 465 ASN C 159
REMARK 465 GLY C 160
REMARK 465 SER C 161
REMARK 465 ASP C 199
REMARK 465 ARG C 200
REMARK 465 PRO C 201
REMARK 465 PRO C 202
REMARK 465 ASP C 203
REMARK 465 ILE C 204
REMARK 465 PRO C 205
REMARK 465 SER C 206
REMARK 465 GLU C 207
REMARK 465 ASP C 208
REMARK 465 LEU C 209
REMARK 465 ASP C 210
REMARK 465 PHE C 211
REMARK 465 LEU C 212
REMARK 465 ASP C 213
REMARK 465 GLY C 214
REMARK 465 HIS C 286
REMARK 465 PRO C 287
REMARK 465 SER C 288
REMARK 465 VAL C 289
REMARK 465 SER C 290
REMARK 465 ARG C 291
REMARK 465 HIS C 292
REMARK 465 GLY C 293
REMARK 465 ASP C 294
REMARK 465 ASN C 295
REMARK 465 GLY C 528
REMARK 465 GLY C 529
REMARK 465 THR C 530
REMARK 465 GLY C 531
REMARK 465 THR C 532
REMARK 465 GLU C 533
REMARK 465 LYS C 534
REMARK 465 SER C 535
REMARK 465 GLU C 536
REMARK 465 LEU C 537
REMARK 465 GLN C 538
REMARK 465 ALA C 539
REMARK 465 TYR C 540
REMARK 465 ILE C 541
REMARK 465 GLU C 542
REMARK 465 GLN C 543
REMARK 465 CYS C 544
REMARK 465 GLN C 545
REMARK 465 ASP C 546
REMARK 465 SER C 547
REMARK 465 PRO C 548
REMARK 465 THR C 549
REMARK 465 SER C 550
REMARK 465 GLY C 551
REMARK 465 LYS C 552
REMARK 465 PHE C 553
REMARK 465 ARG C 554
REMARK 465 ARG C 555
REMARK 465 GLY C 556
REMARK 465 SER C 557
REMARK 465 GLY C 558
REMARK 465 SER C 559
REMARK 465 ALA C 560
REMARK 465 CYS C 561
REMARK 465 SER C 562
REMARK 465 LEU C 563
REMARK 465 PHE C 564
REMARK 465 CYS C 565
REMARK 465 CYS C 566
REMARK 465 CYS C 567
REMARK 465 GLY C 568
REMARK 465 ARG C 569
REMARK 465 ASP C 570
REMARK 465 SER C 571
REMARK 465 PRO C 572
REMARK 465 GLU C 573
REMARK 465 ASP C 574
REMARK 465 HIS C 575
REMARK 465 SER C 576
REMARK 465 LEU C 577
REMARK 465 LEU C 578
REMARK 465 VAL C 579
REMARK 465 ASN C 580
REMARK 465 VAL C 581
REMARK 465 ASP C 582
REMARK 465 GLY C 583
REMARK 465 GLY C 584
REMARK 465 SER C 585
REMARK 465 SER C 586
REMARK 465 GLY C 587
REMARK 465 GLY C 588
REMARK 465 LEU C 589
REMARK 465 VAL C 590
REMARK 465 PRO C 591
REMARK 465 ARG C 592
REMARK 465 MET D 1
REMARK 465 ALA D 2
REMARK 465 THR D 3
REMARK 465 PRO D 4
REMARK 465 ALA D 5
REMARK 465 GLY D 6
REMARK 465 ARG D 7
REMARK 465 ARG D 8
REMARK 465 ALA D 9
REMARK 465 SER D 10
REMARK 465 GLU D 11
REMARK 465 THR D 12
REMARK 465 GLU D 13
REMARK 465 ARG D 14
REMARK 465 LEU D 15
REMARK 465 LEU D 16
REMARK 465 THR D 17
REMARK 465 PRO D 18
REMARK 465 ASN D 19
REMARK 465 PRO D 20
REMARK 465 GLY D 21
REMARK 465 TYR D 22
REMARK 465 GLY D 23
REMARK 465 THR D 24
REMARK 465 GLN D 25
REMARK 465 VAL D 26
REMARK 465 GLY D 27
REMARK 465 THR D 28
REMARK 465 SER D 29
REMARK 465 PRO D 30
REMARK 465 ALA D 31
REMARK 465 PRO D 32
REMARK 465 THR D 33
REMARK 465 THR D 34
REMARK 465 PRO D 35
REMARK 465 THR D 36
REMARK 465 GLU D 37
REMARK 465 GLY D 152
REMARK 465 GLY D 153
REMARK 465 GLY D 154
REMARK 465 GLY D 155
REMARK 465 PRO D 156
REMARK 465 TRP D 157
REMARK 465 ALA D 158
REMARK 465 ASN D 159
REMARK 465 GLY D 160
REMARK 465 SER D 161
REMARK 465 ASP D 199
REMARK 465 ARG D 200
REMARK 465 PRO D 201
REMARK 465 PRO D 202
REMARK 465 ASP D 203
REMARK 465 ILE D 204
REMARK 465 PRO D 205
REMARK 465 SER D 206
REMARK 465 GLU D 207
REMARK 465 ASP D 208
REMARK 465 LEU D 209
REMARK 465 ASP D 210
REMARK 465 PHE D 211
REMARK 465 LEU D 212
REMARK 465 ASP D 213
REMARK 465 GLY D 214
REMARK 465 HIS D 286
REMARK 465 PRO D 287
REMARK 465 SER D 288
REMARK 465 VAL D 289
REMARK 465 SER D 290
REMARK 465 ARG D 291
REMARK 465 HIS D 292
REMARK 465 GLY D 293
REMARK 465 ASP D 294
REMARK 465 ASN D 295
REMARK 465 GLY D 528
REMARK 465 GLY D 529
REMARK 465 THR D 530
REMARK 465 GLY D 531
REMARK 465 THR D 532
REMARK 465 GLU D 533
REMARK 465 LYS D 534
REMARK 465 SER D 535
REMARK 465 GLU D 536
REMARK 465 LEU D 537
REMARK 465 GLN D 538
REMARK 465 ALA D 539
REMARK 465 TYR D 540
REMARK 465 ILE D 541
REMARK 465 GLU D 542
REMARK 465 GLN D 543
REMARK 465 CYS D 544
REMARK 465 GLN D 545
REMARK 465 ASP D 546
REMARK 465 SER D 547
REMARK 465 PRO D 548
REMARK 465 THR D 549
REMARK 465 SER D 550
REMARK 465 GLY D 551
REMARK 465 LYS D 552
REMARK 465 PHE D 553
REMARK 465 ARG D 554
REMARK 465 ARG D 555
REMARK 465 GLY D 556
REMARK 465 SER D 557
REMARK 465 GLY D 558
REMARK 465 SER D 559
REMARK 465 ALA D 560
REMARK 465 CYS D 561
REMARK 465 SER D 562
REMARK 465 LEU D 563
REMARK 465 PHE D 564
REMARK 465 CYS D 565
REMARK 465 CYS D 566
REMARK 465 CYS D 567
REMARK 465 GLY D 568
REMARK 465 ARG D 569
REMARK 465 ASP D 570
REMARK 465 SER D 571
REMARK 465 PRO D 572
REMARK 465 GLU D 573
REMARK 465 ASP D 574
REMARK 465 HIS D 575
REMARK 465 SER D 576
REMARK 465 LEU D 577
REMARK 465 LEU D 578
REMARK 465 VAL D 579
REMARK 465 ASN D 580
REMARK 465 VAL D 581
REMARK 465 ASP D 582
REMARK 465 GLY D 583
REMARK 465 GLY D 584
REMARK 465 SER D 585
REMARK 465 SER D 586
REMARK 465 GLY D 587
REMARK 465 GLY D 588
REMARK 465 LEU D 589
REMARK 465 VAL D 590
REMARK 465 PRO D 591
REMARK 465 ARG D 592
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 ARG A 61 72.24 60.62
REMARK 500 LYS A 62 79.37 -116.16
REMARK 500 THR A 116 50.01 -92.50
REMARK 500 ILE A 142 -61.42 -122.65
REMARK 500 LYS A 219 -166.10 -77.83
REMARK 500 LYS A 227 25.29 -142.42
REMARK 500 ILE A 229 -61.82 -109.27
REMARK 500 GLU A 341 71.70 58.85
REMARK 500 VAL A 351 -76.46 -87.39
REMARK 500 ASN A 352 178.98 176.07
REMARK 500 ASN A 412 47.87 -82.45
REMARK 500 ILE A 413 -24.45 -148.62
REMARK 500 LEU A 414 45.63 37.33
REMARK 500 VAL A 420 -65.15 -91.12
REMARK 500 TYR A 507 -62.95 -93.85
REMARK 500 ARG B 61 72.26 60.55
REMARK 500 LYS B 62 79.37 -116.19
REMARK 500 THR B 116 50.03 -92.49
REMARK 500 ILE B 142 -61.43 -122.67
REMARK 500 LYS B 219 -166.09 -77.71
REMARK 500 LYS B 227 25.35 -142.41
REMARK 500 ILE B 229 -61.94 -109.17
REMARK 500 GLU B 341 71.68 58.79
REMARK 500 VAL B 351 -76.49 -87.36
REMARK 500 ASN B 352 178.98 176.07
REMARK 500 ASN B 412 47.84 -82.48
REMARK 500 ILE B 413 -24.56 -148.61
REMARK 500 LEU B 414 45.57 37.46
REMARK 500 VAL B 420 -65.11 -91.19
REMARK 500 TYR B 507 -62.97 -93.86
REMARK 500 ARG C 61 72.31 60.52
REMARK 500 LYS C 62 79.36 -116.16
REMARK 500 THR C 116 49.99 -92.45
REMARK 500 ILE C 142 -61.39 -122.63
REMARK 500 LYS C 219 -166.09 -77.81
REMARK 500 LYS C 227 25.24 -142.38
REMARK 500 ILE C 229 -61.88 -109.18
REMARK 500 GLU C 341 71.71 58.82
REMARK 500 VAL C 351 -76.45 -87.32
REMARK 500 ASN C 352 178.91 176.05
REMARK 500 ASN C 412 47.94 -82.52
REMARK 500 ILE C 413 -24.55 -148.57
REMARK 500 LEU C 414 45.66 37.38
REMARK 500 VAL C 420 -65.14 -91.11
REMARK 500 TYR C 507 -62.93 -93.83
REMARK 500 ARG D 61 72.31 60.58
REMARK 500 LYS D 62 79.32 -116.17
REMARK 500 THR D 116 49.93 -92.39
REMARK 500 ILE D 142 -61.47 -122.60
REMARK 500 LYS D 219 -166.05 -77.82
REMARK 500
REMARK 500 THIS ENTRY HAS 60 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION. CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS. TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500 MODEL OMEGA
REMARK 500 TYR A 411 ASN A 412 -146.83
REMARK 500 ASN A 412 ILE A 413 125.16
REMARK 500 TYR B 411 ASN B 412 -146.86
REMARK 500 ASN B 412 ILE B 413 125.24
REMARK 500 TYR C 411 ASN C 412 -146.80
REMARK 500 ASN C 412 ILE C 413 125.16
REMARK 500 TYR D 411 ASN D 412 -146.83
REMARK 500 ASN D 412 ILE D 413 125.16
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: EMD-8882 RELATED DB: EMDB
REMARK 900 RELATED ID: EMD-8881 RELATED DB: EMDB
REMARK 900 RELATED ID: EMD-8883 RELATED DB: EMDB
REMARK 900 RELATED ID: 5WPO RELATED DB: PDB
REMARK 900 RELATED ID: 5WPQ RELATED DB: PDB
REMARK 900 RELATED ID: 5WPV RELATED DB: PDB
DBREF 5WPT A 1 580 UNP Q99J21 MCLN1_MOUSE 1 580
DBREF 5WPT B 1 580 UNP Q99J21 MCLN1_MOUSE 1 580
DBREF 5WPT C 1 580 UNP Q99J21 MCLN1_MOUSE 1 580
DBREF 5WPT D 1 580 UNP Q99J21 MCLN1_MOUSE 1 580
SEQADV 5WPT VAL A 581 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ASP A 582 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY A 583 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY A 584 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER A 585 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER A 586 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY A 587 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY A 588 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT LEU A 589 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL A 590 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT PRO A 591 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ARG A 592 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL B 581 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ASP B 582 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY B 583 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY B 584 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER B 585 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER B 586 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY B 587 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY B 588 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT LEU B 589 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL B 590 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT PRO B 591 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ARG B 592 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL C 581 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ASP C 582 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY C 583 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY C 584 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER C 585 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER C 586 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY C 587 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY C 588 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT LEU C 589 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL C 590 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT PRO C 591 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ARG C 592 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL D 581 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ASP D 582 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY D 583 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY D 584 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER D 585 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT SER D 586 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY D 587 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT GLY D 588 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT LEU D 589 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT VAL D 590 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT PRO D 591 UNP Q99J21 EXPRESSION TAG
SEQADV 5WPT ARG D 592 UNP Q99J21 EXPRESSION TAG
SEQRES 1 A 592 MET ALA THR PRO ALA GLY ARG ARG ALA SER GLU THR GLU
SEQRES 2 A 592 ARG LEU LEU THR PRO ASN PRO GLY TYR GLY THR GLN VAL
SEQRES 3 A 592 GLY THR SER PRO ALA PRO THR THR PRO THR GLU GLU GLU
SEQRES 4 A 592 ASP LEU ARG ARG ARG LEU LYS TYR PHE PHE MET SER PRO
SEQRES 5 A 592 CYS ASP LYS PHE ARG ALA LYS GLY ARG LYS PRO CYS LYS
SEQRES 6 A 592 LEU MET LEU GLN VAL VAL LYS ILE LEU VAL VAL THR VAL
SEQRES 7 A 592 GLN LEU ILE LEU PHE GLY LEU SER ASN GLN LEU VAL VAL
SEQRES 8 A 592 THR PHE ARG GLU GLU ASN THR ILE ALA PHE ARG HIS LEU
SEQRES 9 A 592 PHE LEU LEU GLY TYR SER ASP GLY SER ASP ASP THR PHE
SEQRES 10 A 592 ALA ALA TYR THR GLN GLU GLN LEU TYR GLN ALA ILE PHE
SEQRES 11 A 592 TYR ALA VAL ASP GLN TYR LEU ILE LEU PRO GLU ILE SER
SEQRES 12 A 592 LEU GLY ARG TYR ALA TYR VAL ARG GLY GLY GLY GLY PRO
SEQRES 13 A 592 TRP ALA ASN GLY SER ALA LEU ALA LEU CYS GLN ARG TYR
SEQRES 14 A 592 TYR HIS ARG GLY HIS VAL ASP PRO ALA ASN ASP THR PHE
SEQRES 15 A 592 ASP ILE ASP PRO ARG VAL VAL THR ASP CYS ILE GLN VAL
SEQRES 16 A 592 ASP PRO PRO ASP ARG PRO PRO ASP ILE PRO SER GLU ASP
SEQRES 17 A 592 LEU ASP PHE LEU ASP GLY SER ALA SER TYR LYS ASN LEU
SEQRES 18 A 592 THR LEU LYS PHE HIS LYS LEU ILE ASN VAL THR ILE HIS
SEQRES 19 A 592 PHE GLN LEU LYS THR ILE ASN LEU GLN SER LEU ILE ASN
SEQRES 20 A 592 ASN GLU ILE PRO ASP CYS TYR THR PHE SER ILE LEU ILE
SEQRES 21 A 592 THR PHE ASP ASN LYS ALA HIS SER GLY ARG ILE PRO ILE
SEQRES 22 A 592 ARG LEU GLU THR LYS THR HIS ILE GLN GLU CYS LYS HIS
SEQRES 23 A 592 PRO SER VAL SER ARG HIS GLY ASP ASN SER PHE ARG LEU
SEQRES 24 A 592 LEU PHE ASP VAL VAL VAL ILE LEU THR CYS SER LEU SER
SEQRES 25 A 592 PHE LEU LEU CYS ALA ARG SER LEU LEU ARG GLY PHE LEU
SEQRES 26 A 592 LEU GLN ASN GLU PHE VAL VAL PHE MET TRP ARG ARG ARG
SEQRES 27 A 592 GLY ARG GLU ILE SER LEU TRP GLU ARG LEU GLU PHE VAL
SEQRES 28 A 592 ASN GLY TRP TYR ILE LEU LEU VAL THR SER ASP VAL LEU
SEQRES 29 A 592 THR ILE SER GLY THR VAL MET LYS ILE GLY ILE GLU ALA
SEQRES 30 A 592 LYS ASN LEU ALA SER TYR ASP VAL CYS SER ILE LEU LEU
SEQRES 31 A 592 GLY THR SER THR LEU LEU VAL TRP VAL GLY VAL ILE ARG
SEQRES 32 A 592 TYR LEU THR PHE PHE HIS LYS TYR ASN ILE LEU ILE ALA
SEQRES 33 A 592 THR LEU ARG VAL ALA LEU PRO SER VAL MET ARG PHE CYS
SEQRES 34 A 592 CYS CYS VAL ALA VAL ILE TYR LEU GLY TYR CYS PHE CYS
SEQRES 35 A 592 GLY TRP ILE VAL LEU GLY PRO TYR HIS VAL LYS PHE ARG
SEQRES 36 A 592 SER LEU SER MET VAL SER GLU CYS LEU PHE SER LEU ILE
SEQRES 37 A 592 ASN GLY ASP ASP MET PHE VAL THR PHE ALA ALA MET GLN
SEQRES 38 A 592 ALA GLN GLN GLY HIS SER SER LEU VAL TRP LEU PHE SER
SEQRES 39 A 592 GLN LEU TYR LEU TYR SER PHE ILE SER LEU PHE ILE TYR
SEQRES 40 A 592 MET VAL LEU SER LEU PHE ILE ALA LEU ILE THR GLY ALA
SEQRES 41 A 592 TYR ASP THR ILE LYS HIS PRO GLY GLY THR GLY THR GLU
SEQRES 42 A 592 LYS SER GLU LEU GLN ALA TYR ILE GLU GLN CYS GLN ASP
SEQRES 43 A 592 SER PRO THR SER GLY LYS PHE ARG ARG GLY SER GLY SER
SEQRES 44 A 592 ALA CYS SER LEU PHE CYS CYS CYS GLY ARG ASP SER PRO
SEQRES 45 A 592 GLU ASP HIS SER LEU LEU VAL ASN VAL ASP GLY GLY SER
SEQRES 46 A 592 SER GLY GLY LEU VAL PRO ARG
SEQRES 1 B 592 MET ALA THR PRO ALA GLY ARG ARG ALA SER GLU THR GLU
SEQRES 2 B 592 ARG LEU LEU THR PRO ASN PRO GLY TYR GLY THR GLN VAL
SEQRES 3 B 592 GLY THR SER PRO ALA PRO THR THR PRO THR GLU GLU GLU
SEQRES 4 B 592 ASP LEU ARG ARG ARG LEU LYS TYR PHE PHE MET SER PRO
SEQRES 5 B 592 CYS ASP LYS PHE ARG ALA LYS GLY ARG LYS PRO CYS LYS
SEQRES 6 B 592 LEU MET LEU GLN VAL VAL LYS ILE LEU VAL VAL THR VAL
SEQRES 7 B 592 GLN LEU ILE LEU PHE GLY LEU SER ASN GLN LEU VAL VAL
SEQRES 8 B 592 THR PHE ARG GLU GLU ASN THR ILE ALA PHE ARG HIS LEU
SEQRES 9 B 592 PHE LEU LEU GLY TYR SER ASP GLY SER ASP ASP THR PHE
SEQRES 10 B 592 ALA ALA TYR THR GLN GLU GLN LEU TYR GLN ALA ILE PHE
SEQRES 11 B 592 TYR ALA VAL ASP GLN TYR LEU ILE LEU PRO GLU ILE SER
SEQRES 12 B 592 LEU GLY ARG TYR ALA TYR VAL ARG GLY GLY GLY GLY PRO
SEQRES 13 B 592 TRP ALA ASN GLY SER ALA LEU ALA LEU CYS GLN ARG TYR
SEQRES 14 B 592 TYR HIS ARG GLY HIS VAL ASP PRO ALA ASN ASP THR PHE
SEQRES 15 B 592 ASP ILE ASP PRO ARG VAL VAL THR ASP CYS ILE GLN VAL
SEQRES 16 B 592 ASP PRO PRO ASP ARG PRO PRO ASP ILE PRO SER GLU ASP
SEQRES 17 B 592 LEU ASP PHE LEU ASP GLY SER ALA SER TYR LYS ASN LEU
SEQRES 18 B 592 THR LEU LYS PHE HIS LYS LEU ILE ASN VAL THR ILE HIS
SEQRES 19 B 592 PHE GLN LEU LYS THR ILE ASN LEU GLN SER LEU ILE ASN
SEQRES 20 B 592 ASN GLU ILE PRO ASP CYS TYR THR PHE SER ILE LEU ILE
SEQRES 21 B 592 THR PHE ASP ASN LYS ALA HIS SER GLY ARG ILE PRO ILE
SEQRES 22 B 592 ARG LEU GLU THR LYS THR HIS ILE GLN GLU CYS LYS HIS
SEQRES 23 B 592 PRO SER VAL SER ARG HIS GLY ASP ASN SER PHE ARG LEU
SEQRES 24 B 592 LEU PHE ASP VAL VAL VAL ILE LEU THR CYS SER LEU SER
SEQRES 25 B 592 PHE LEU LEU CYS ALA ARG SER LEU LEU ARG GLY PHE LEU
SEQRES 26 B 592 LEU GLN ASN GLU PHE VAL VAL PHE MET TRP ARG ARG ARG
SEQRES 27 B 592 GLY ARG GLU ILE SER LEU TRP GLU ARG LEU GLU PHE VAL
SEQRES 28 B 592 ASN GLY TRP TYR ILE LEU LEU VAL THR SER ASP VAL LEU
SEQRES 29 B 592 THR ILE SER GLY THR VAL MET LYS ILE GLY ILE GLU ALA
SEQRES 30 B 592 LYS ASN LEU ALA SER TYR ASP VAL CYS SER ILE LEU LEU
SEQRES 31 B 592 GLY THR SER THR LEU LEU VAL TRP VAL GLY VAL ILE ARG
SEQRES 32 B 592 TYR LEU THR PHE PHE HIS LYS TYR ASN ILE LEU ILE ALA
SEQRES 33 B 592 THR LEU ARG VAL ALA LEU PRO SER VAL MET ARG PHE CYS
SEQRES 34 B 592 CYS CYS VAL ALA VAL ILE TYR LEU GLY TYR CYS PHE CYS
SEQRES 35 B 592 GLY TRP ILE VAL LEU GLY PRO TYR HIS VAL LYS PHE ARG
SEQRES 36 B 592 SER LEU SER MET VAL SER GLU CYS LEU PHE SER LEU ILE
SEQRES 37 B 592 ASN GLY ASP ASP MET PHE VAL THR PHE ALA ALA MET GLN
SEQRES 38 B 592 ALA GLN GLN GLY HIS SER SER LEU VAL TRP LEU PHE SER
SEQRES 39 B 592 GLN LEU TYR LEU TYR SER PHE ILE SER LEU PHE ILE TYR
SEQRES 40 B 592 MET VAL LEU SER LEU PHE ILE ALA LEU ILE THR GLY ALA
SEQRES 41 B 592 TYR ASP THR ILE LYS HIS PRO GLY GLY THR GLY THR GLU
SEQRES 42 B 592 LYS SER GLU LEU GLN ALA TYR ILE GLU GLN CYS GLN ASP
SEQRES 43 B 592 SER PRO THR SER GLY LYS PHE ARG ARG GLY SER GLY SER
SEQRES 44 B 592 ALA CYS SER LEU PHE CYS CYS CYS GLY ARG ASP SER PRO
SEQRES 45 B 592 GLU ASP HIS SER LEU LEU VAL ASN VAL ASP GLY GLY SER
SEQRES 46 B 592 SER GLY GLY LEU VAL PRO ARG
SEQRES 1 C 592 MET ALA THR PRO ALA GLY ARG ARG ALA SER GLU THR GLU
SEQRES 2 C 592 ARG LEU LEU THR PRO ASN PRO GLY TYR GLY THR GLN VAL
SEQRES 3 C 592 GLY THR SER PRO ALA PRO THR THR PRO THR GLU GLU GLU
SEQRES 4 C 592 ASP LEU ARG ARG ARG LEU LYS TYR PHE PHE MET SER PRO
SEQRES 5 C 592 CYS ASP LYS PHE ARG ALA LYS GLY ARG LYS PRO CYS LYS
SEQRES 6 C 592 LEU MET LEU GLN VAL VAL LYS ILE LEU VAL VAL THR VAL
SEQRES 7 C 592 GLN LEU ILE LEU PHE GLY LEU SER ASN GLN LEU VAL VAL
SEQRES 8 C 592 THR PHE ARG GLU GLU ASN THR ILE ALA PHE ARG HIS LEU
SEQRES 9 C 592 PHE LEU LEU GLY TYR SER ASP GLY SER ASP ASP THR PHE
SEQRES 10 C 592 ALA ALA TYR THR GLN GLU GLN LEU TYR GLN ALA ILE PHE
SEQRES 11 C 592 TYR ALA VAL ASP GLN TYR LEU ILE LEU PRO GLU ILE SER
SEQRES 12 C 592 LEU GLY ARG TYR ALA TYR VAL ARG GLY GLY GLY GLY PRO
SEQRES 13 C 592 TRP ALA ASN GLY SER ALA LEU ALA LEU CYS GLN ARG TYR
SEQRES 14 C 592 TYR HIS ARG GLY HIS VAL ASP PRO ALA ASN ASP THR PHE
SEQRES 15 C 592 ASP ILE ASP PRO ARG VAL VAL THR ASP CYS ILE GLN VAL
SEQRES 16 C 592 ASP PRO PRO ASP ARG PRO PRO ASP ILE PRO SER GLU ASP
SEQRES 17 C 592 LEU ASP PHE LEU ASP GLY SER ALA SER TYR LYS ASN LEU
SEQRES 18 C 592 THR LEU LYS PHE HIS LYS LEU ILE ASN VAL THR ILE HIS
SEQRES 19 C 592 PHE GLN LEU LYS THR ILE ASN LEU GLN SER LEU ILE ASN
SEQRES 20 C 592 ASN GLU ILE PRO ASP CYS TYR THR PHE SER ILE LEU ILE
SEQRES 21 C 592 THR PHE ASP ASN LYS ALA HIS SER GLY ARG ILE PRO ILE
SEQRES 22 C 592 ARG LEU GLU THR LYS THR HIS ILE GLN GLU CYS LYS HIS
SEQRES 23 C 592 PRO SER VAL SER ARG HIS GLY ASP ASN SER PHE ARG LEU
SEQRES 24 C 592 LEU PHE ASP VAL VAL VAL ILE LEU THR CYS SER LEU SER
SEQRES 25 C 592 PHE LEU LEU CYS ALA ARG SER LEU LEU ARG GLY PHE LEU
SEQRES 26 C 592 LEU GLN ASN GLU PHE VAL VAL PHE MET TRP ARG ARG ARG
SEQRES 27 C 592 GLY ARG GLU ILE SER LEU TRP GLU ARG LEU GLU PHE VAL
SEQRES 28 C 592 ASN GLY TRP TYR ILE LEU LEU VAL THR SER ASP VAL LEU
SEQRES 29 C 592 THR ILE SER GLY THR VAL MET LYS ILE GLY ILE GLU ALA
SEQRES 30 C 592 LYS ASN LEU ALA SER TYR ASP VAL CYS SER ILE LEU LEU
SEQRES 31 C 592 GLY THR SER THR LEU LEU VAL TRP VAL GLY VAL ILE ARG
SEQRES 32 C 592 TYR LEU THR PHE PHE HIS LYS TYR ASN ILE LEU ILE ALA
SEQRES 33 C 592 THR LEU ARG VAL ALA LEU PRO SER VAL MET ARG PHE CYS
SEQRES 34 C 592 CYS CYS VAL ALA VAL ILE TYR LEU GLY TYR CYS PHE CYS
SEQRES 35 C 592 GLY TRP ILE VAL LEU GLY PRO TYR HIS VAL LYS PHE ARG
SEQRES 36 C 592 SER LEU SER MET VAL SER GLU CYS LEU PHE SER LEU ILE
SEQRES 37 C 592 ASN GLY ASP ASP MET PHE VAL THR PHE ALA ALA MET GLN
SEQRES 38 C 592 ALA GLN GLN GLY HIS SER SER LEU VAL TRP LEU PHE SER
SEQRES 39 C 592 GLN LEU TYR LEU TYR SER PHE ILE SER LEU PHE ILE TYR
SEQRES 40 C 592 MET VAL LEU SER LEU PHE ILE ALA LEU ILE THR GLY ALA
SEQRES 41 C 592 TYR ASP THR ILE LYS HIS PRO GLY GLY THR GLY THR GLU
SEQRES 42 C 592 LYS SER GLU LEU GLN ALA TYR ILE GLU GLN CYS GLN ASP
SEQRES 43 C 592 SER PRO THR SER GLY LYS PHE ARG ARG GLY SER GLY SER
SEQRES 44 C 592 ALA CYS SER LEU PHE CYS CYS CYS GLY ARG ASP SER PRO
SEQRES 45 C 592 GLU ASP HIS SER LEU LEU VAL ASN VAL ASP GLY GLY SER
SEQRES 46 C 592 SER GLY GLY LEU VAL PRO ARG
SEQRES 1 D 592 MET ALA THR PRO ALA GLY ARG ARG ALA SER GLU THR GLU
SEQRES 2 D 592 ARG LEU LEU THR PRO ASN PRO GLY TYR GLY THR GLN VAL
SEQRES 3 D 592 GLY THR SER PRO ALA PRO THR THR PRO THR GLU GLU GLU
SEQRES 4 D 592 ASP LEU ARG ARG ARG LEU LYS TYR PHE PHE MET SER PRO
SEQRES 5 D 592 CYS ASP LYS PHE ARG ALA LYS GLY ARG LYS PRO CYS LYS
SEQRES 6 D 592 LEU MET LEU GLN VAL VAL LYS ILE LEU VAL VAL THR VAL
SEQRES 7 D 592 GLN LEU ILE LEU PHE GLY LEU SER ASN GLN LEU VAL VAL
SEQRES 8 D 592 THR PHE ARG GLU GLU ASN THR ILE ALA PHE ARG HIS LEU
SEQRES 9 D 592 PHE LEU LEU GLY TYR SER ASP GLY SER ASP ASP THR PHE
SEQRES 10 D 592 ALA ALA TYR THR GLN GLU GLN LEU TYR GLN ALA ILE PHE
SEQRES 11 D 592 TYR ALA VAL ASP GLN TYR LEU ILE LEU PRO GLU ILE SER
SEQRES 12 D 592 LEU GLY ARG TYR ALA TYR VAL ARG GLY GLY GLY GLY PRO
SEQRES 13 D 592 TRP ALA ASN GLY SER ALA LEU ALA LEU CYS GLN ARG TYR
SEQRES 14 D 592 TYR HIS ARG GLY HIS VAL ASP PRO ALA ASN ASP THR PHE
SEQRES 15 D 592 ASP ILE ASP PRO ARG VAL VAL THR ASP CYS ILE GLN VAL
SEQRES 16 D 592 ASP PRO PRO ASP ARG PRO PRO ASP ILE PRO SER GLU ASP
SEQRES 17 D 592 LEU ASP PHE LEU ASP GLY SER ALA SER TYR LYS ASN LEU
SEQRES 18 D 592 THR LEU LYS PHE HIS LYS LEU ILE ASN VAL THR ILE HIS
SEQRES 19 D 592 PHE GLN LEU LYS THR ILE ASN LEU GLN SER LEU ILE ASN
SEQRES 20 D 592 ASN GLU ILE PRO ASP CYS TYR THR PHE SER ILE LEU ILE
SEQRES 21 D 592 THR PHE ASP ASN LYS ALA HIS SER GLY ARG ILE PRO ILE
SEQRES 22 D 592 ARG LEU GLU THR LYS THR HIS ILE GLN GLU CYS LYS HIS
SEQRES 23 D 592 PRO SER VAL SER ARG HIS GLY ASP ASN SER PHE ARG LEU
SEQRES 24 D 592 LEU PHE ASP VAL VAL VAL ILE LEU THR CYS SER LEU SER
SEQRES 25 D 592 PHE LEU LEU CYS ALA ARG SER LEU LEU ARG GLY PHE LEU
SEQRES 26 D 592 LEU GLN ASN GLU PHE VAL VAL PHE MET TRP ARG ARG ARG
SEQRES 27 D 592 GLY ARG GLU ILE SER LEU TRP GLU ARG LEU GLU PHE VAL
SEQRES 28 D 592 ASN GLY TRP TYR ILE LEU LEU VAL THR SER ASP VAL LEU
SEQRES 29 D 592 THR ILE SER GLY THR VAL MET LYS ILE GLY ILE GLU ALA
SEQRES 30 D 592 LYS ASN LEU ALA SER TYR ASP VAL CYS SER ILE LEU LEU
SEQRES 31 D 592 GLY THR SER THR LEU LEU VAL TRP VAL GLY VAL ILE ARG
SEQRES 32 D 592 TYR LEU THR PHE PHE HIS LYS TYR ASN ILE LEU ILE ALA
SEQRES 33 D 592 THR LEU ARG VAL ALA LEU PRO SER VAL MET ARG PHE CYS
SEQRES 34 D 592 CYS CYS VAL ALA VAL ILE TYR LEU GLY TYR CYS PHE CYS
SEQRES 35 D 592 GLY TRP ILE VAL LEU GLY PRO TYR HIS VAL LYS PHE ARG
SEQRES 36 D 592 SER LEU SER MET VAL SER GLU CYS LEU PHE SER LEU ILE
SEQRES 37 D 592 ASN GLY ASP ASP MET PHE VAL THR PHE ALA ALA MET GLN
SEQRES 38 D 592 ALA GLN GLN GLY HIS SER SER LEU VAL TRP LEU PHE SER
SEQRES 39 D 592 GLN LEU TYR LEU TYR SER PHE ILE SER LEU PHE ILE TYR
SEQRES 40 D 592 MET VAL LEU SER LEU PHE ILE ALA LEU ILE THR GLY ALA
SEQRES 41 D 592 TYR ASP THR ILE LYS HIS PRO GLY GLY THR GLY THR GLU
SEQRES 42 D 592 LYS SER GLU LEU GLN ALA TYR ILE GLU GLN CYS GLN ASP
SEQRES 43 D 592 SER PRO THR SER GLY LYS PHE ARG ARG GLY SER GLY SER
SEQRES 44 D 592 ALA CYS SER LEU PHE CYS CYS CYS GLY ARG ASP SER PRO
SEQRES 45 D 592 GLU ASP HIS SER LEU LEU VAL ASN VAL ASP GLY GLY SER
SEQRES 46 D 592 SER GLY GLY LEU VAL PRO ARG
HET NAG E 1 14
HET NAG E 2 14
HET NAG F 1 14
HET NAG F 2 14
HET NAG G 1 14
HET NAG G 2 14
HET NAG H 1 14
HET NAG H 2 14
HET NA A 603 1
HETNAM NAG 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE
HETNAM NA SODIUM ION
HETSYN NAG N-ACETYL-BETA-D-GLUCOSAMINE; 2-ACETAMIDO-2-DEOXY-BETA-
HETSYN 2 NAG D-GLUCOSE; 2-ACETAMIDO-2-DEOXY-D-GLUCOSE; 2-ACETAMIDO-
HETSYN 3 NAG 2-DEOXY-GLUCOSE; N-ACETYL-D-GLUCOSAMINE
FORMUL 5 NAG 8(C8 H15 N O6)
FORMUL 9 NA NA 1+
HELIX 1 AA1 GLU A 38 PHE A 49 1 12
HELIX 2 AA2 SER A 51 ARG A 57 1 7
HELIX 3 AA3 PRO A 63 LEU A 106 1 44
HELIX 4 AA4 GLN A 122 ILE A 138 1 17
HELIX 5 AA5 LEU A 139 ILE A 142 5 4
HELIX 6 AA6 LEU A 242 ILE A 246 5 5
HELIX 7 AA7 PHE A 297 ARG A 337 1 41
HELIX 8 AA8 SER A 343 LEU A 348 1 6
HELIX 9 AA9 GLU A 349 VAL A 351 5 3
HELIX 10 AB1 TRP A 354 GLU A 376 1 23
HELIX 11 AB2 ASP A 384 GLY A 400 1 17
HELIX 12 AB3 VAL A 401 PHE A 407 5 7
HELIX 13 AB4 ILE A 415 ARG A 419 5 5
HELIX 14 AB5 VAL A 420 LEU A 447 1 28
HELIX 15 AB6 SER A 456 GLY A 470 1 15
HELIX 16 AB7 ASP A 472 GLN A 483 1 12
HELIX 17 AB8 GLN A 484 SER A 487 5 4
HELIX 18 AB9 LEU A 489 TYR A 507 1 19
HELIX 19 AC1 VAL A 509 HIS A 526 1 18
HELIX 20 AC2 GLU B 39 PHE B 49 1 11
HELIX 21 AC3 SER B 51 ARG B 57 1 7
HELIX 22 AC4 PRO B 63 LEU B 106 1 44
HELIX 23 AC5 GLN B 122 ILE B 138 1 17
HELIX 24 AC6 LEU B 139 ILE B 142 5 4
HELIX 25 AC7 LEU B 242 ILE B 246 5 5
HELIX 26 AC8 PHE B 297 ARG B 337 1 41
HELIX 27 AC9 SER B 343 LEU B 348 1 6
HELIX 28 AD1 GLU B 349 VAL B 351 5 3
HELIX 29 AD2 TRP B 354 GLU B 376 1 23
HELIX 30 AD3 ASP B 384 GLY B 400 1 17
HELIX 31 AD4 VAL B 401 PHE B 407 5 7
HELIX 32 AD5 ILE B 415 ARG B 419 5 5
HELIX 33 AD6 VAL B 420 LEU B 447 1 28
HELIX 34 AD7 SER B 456 GLY B 470 1 15
HELIX 35 AD8 ASP B 472 GLN B 483 1 12
HELIX 36 AD9 GLN B 484 SER B 487 5 4
HELIX 37 AE1 LEU B 489 TYR B 507 1 19
HELIX 38 AE2 VAL B 509 HIS B 526 1 18
HELIX 39 AE3 GLU C 39 PHE C 49 1 11
HELIX 40 AE4 SER C 51 ARG C 57 1 7
HELIX 41 AE5 PRO C 63 LEU C 106 1 44
HELIX 42 AE6 GLN C 122 ILE C 138 1 17
HELIX 43 AE7 LEU C 139 ILE C 142 5 4
HELIX 44 AE8 LEU C 242 ILE C 246 5 5
HELIX 45 AE9 PHE C 297 ARG C 337 1 41
HELIX 46 AF1 SER C 343 LEU C 348 1 6
HELIX 47 AF2 GLU C 349 VAL C 351 5 3
HELIX 48 AF3 TRP C 354 GLU C 376 1 23
HELIX 49 AF4 ASP C 384 GLY C 400 1 17
HELIX 50 AF5 VAL C 401 PHE C 407 5 7
HELIX 51 AF6 ILE C 415 ARG C 419 5 5
HELIX 52 AF7 VAL C 420 LEU C 447 1 28
HELIX 53 AF8 SER C 456 GLY C 470 1 15
HELIX 54 AF9 ASP C 472 GLN C 483 1 12
HELIX 55 AG1 GLN C 484 SER C 487 5 4
HELIX 56 AG2 LEU C 489 TYR C 507 1 19
HELIX 57 AG3 VAL C 509 HIS C 526 1 18
HELIX 58 AG4 GLU D 39 PHE D 49 1 11
HELIX 59 AG5 SER D 51 ARG D 57 1 7
HELIX 60 AG6 PRO D 63 LEU D 106 1 44
HELIX 61 AG7 GLN D 122 ILE D 138 1 17
HELIX 62 AG8 LEU D 139 ILE D 142 5 4
HELIX 63 AG9 LEU D 242 ILE D 246 5 5
HELIX 64 AH1 PHE D 297 ARG D 337 1 41
HELIX 65 AH2 SER D 343 LEU D 348 1 6
HELIX 66 AH3 GLU D 349 VAL D 351 5 3
HELIX 67 AH4 TRP D 354 GLU D 376 1 23
HELIX 68 AH5 ASP D 384 GLY D 400 1 17
HELIX 69 AH6 VAL D 401 PHE D 407 5 7
HELIX 70 AH7 ILE D 415 ARG D 419 5 5
HELIX 71 AH8 VAL D 420 LEU D 447 1 28
HELIX 72 AH9 SER D 456 GLY D 470 1 15
HELIX 73 AI1 ASP D 472 GLN D 483 1 12
HELIX 74 AI2 GLN D 484 SER D 487 5 4
HELIX 75 AI3 LEU D 489 TYR D 507 1 19
HELIX 76 AI4 VAL D 509 HIS D 526 1 18
SHEET 1 AA1 6 ALA A 118 ALA A 119 0
SHEET 2 AA1 6 ILE A 271 GLU A 276 -1 O ILE A 271 N ALA A 119
SHEET 3 AA1 6 CYS A 253 ASP A 263 -1 N THR A 261 O ARG A 274
SHEET 4 AA1 6 LEU A 228 ILE A 240 -1 N LEU A 237 O PHE A 256
SHEET 5 AA1 6 LEU A 163 ARG A 172 -1 N ALA A 164 O HIS A 234
SHEET 6 AA1 6 ASP A 185 VAL A 195 -1 O ASP A 191 N GLN A 167
SHEET 1 AA2 6 ALA B 118 ALA B 119 0
SHEET 2 AA2 6 ILE B 271 GLU B 276 -1 O ILE B 271 N ALA B 119
SHEET 3 AA2 6 CYS B 253 ASP B 263 -1 N THR B 261 O ARG B 274
SHEET 4 AA2 6 LEU B 228 ILE B 240 -1 N LEU B 237 O PHE B 256
SHEET 5 AA2 6 LEU B 163 ARG B 172 -1 N ALA B 164 O HIS B 234
SHEET 6 AA2 6 ASP B 185 VAL B 195 -1 O ASP B 191 N GLN B 167
SHEET 1 AA3 6 ALA C 118 ALA C 119 0
SHEET 2 AA3 6 ILE C 271 GLU C 276 -1 O ILE C 271 N ALA C 119
SHEET 3 AA3 6 CYS C 253 ASP C 263 -1 N THR C 261 O ARG C 274
SHEET 4 AA3 6 LEU C 228 ILE C 240 -1 N LEU C 237 O PHE C 256
SHEET 5 AA3 6 LEU C 163 ARG C 172 -1 N ALA C 164 O HIS C 234
SHEET 6 AA3 6 ASP C 185 VAL C 195 -1 O ASP C 191 N GLN C 167
SHEET 1 AA4 6 ALA D 118 ALA D 119 0
SHEET 2 AA4 6 ILE D 271 GLU D 276 -1 O ILE D 271 N ALA D 119
SHEET 3 AA4 6 CYS D 253 ASP D 263 -1 N THR D 261 O ARG D 274
SHEET 4 AA4 6 LEU D 228 ILE D 240 -1 N LEU D 237 O PHE D 256
SHEET 5 AA4 6 LEU D 163 ARG D 172 -1 N ALA D 164 O HIS D 234
SHEET 6 AA4 6 ASP D 185 VAL D 195 -1 O ASP D 191 N GLN D 167
LINK ND2 ASN A 230 C1 NAG E 1 1555 1555 1.44
LINK ND2 ASN B 230 C1 NAG F 1 1555 1555 1.44
LINK ND2 ASN C 230 C1 NAG G 1 1555 1555 1.44
LINK ND2 ASN D 230 C1 NAG H 1 1555 1555 1.44
LINK O4 NAG E 1 C1 NAG E 2 1555 1555 1.45
LINK O4 NAG F 1 C1 NAG F 2 1555 1555 1.45
LINK O4 NAG G 1 C1 NAG G 2 1555 1555 1.45
LINK O4 NAG H 1 C1 NAG H 2 1555 1555 1.45
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
