HEADER TRANSCRIPTION 08-AUG-17 6ALY
TITLE SOLUTION STRUCTURE OF YEAST MED15 ABD2 RESIDUES 277-368
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: UNP RESIDUES 277-368;
COMPND 5 SYNONYM: AUTONOMOUS REPLICATION REGULATORY PROTEIN 3,BASAL EXPRESSION
COMPND 6 ACTIVATOR PROTEIN 1,DEFECTIVE SILENCING SUPPRESSOR PROTEIN 4,MEDIATOR
COMPND 7 COMPLEX SUBUNIT 15,TRANSCRIPTION REGULATORY PROTEIN GAL11,TY
COMPND 8 INSERTION SUPPRESSOR PROTEIN 13;
COMPND 9 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;
SOURCE 3 ORGANISM_COMMON: BAKER'S YEAST;
SOURCE 4 ORGANISM_TAXID: 4932;
SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS MEDIATOR, TRANSCRIPTION ACTIVATION, HELICAL, ABD, MED15, GAL11,
KEYWDS 2 YEAST, TRANSCRIPTION
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR L.M.TUTTLE,D.PACHECO,L.WARFIELD,S.HAHN,R.E.KLEVIT
REVDAT 5 14-JUN-23 6ALY 1 REMARK
REVDAT 4 01-JAN-20 6ALY 1 REMARK
REVDAT 3 20-FEB-19 6ALY 1 REMARK
REVDAT 2 04-APR-18 6ALY 1 JRNL
REVDAT 1 21-MAR-18 6ALY 0
JRNL AUTH L.M.TUTTLE,D.PACHECO,L.WARFIELD,J.LUO,J.RANISH,S.HAHN,
JRNL AUTH 2 R.E.KLEVIT
JRNL TITL GCN4-MEDIATOR SPECIFICITY IS MEDIATED BY A LARGE AND DYNAMIC
JRNL TITL 2 FUZZY PROTEIN-PROTEIN COMPLEX.
JRNL REF CELL REP V. 22 3251 2018
JRNL REFN ESSN 2211-1247
JRNL PMID 29562181
JRNL DOI 10.1016/J.CELREP.2018.02.097
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : X-PLOR NIH 2.45
REMARK 3 AUTHORS : SCHWIETERS, KUSZEWSKI, TJANDRA AND CLORE
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 6ALY COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 10-AUG-17.
REMARK 100 THE DEPOSITION ID IS D_1000228302.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 6.5
REMARK 210 IONIC STRENGTH : 150
REMARK 210 PRESSURE : 1 ATM
REMARK 210 SAMPLE CONTENTS : 20 MM SODIUM PHOSPHATE, 150 MM
REMARK 210 SODIUM CHLORIDE, 0.1 MM EDTA,
REMARK 210 0.1 MM PMSF, 5 MM DTT, 500 UM [U-
REMARK 210 15N] MED15 ABD2, 90% H2O/10% D2O;
REMARK 210 20 MM SODIUM PHOSPHATE, 150 MM
REMARK 210 SODIUM CHLORIDE, 0.1 MM EDTA,
REMARK 210 0.1 MM PMSF, 5 MM DTT, 500 UM [U-
REMARK 210 13C; U-15N] MED15 ABD2, 90% H2O/
REMARK 210 10% D2O; 20 MM SODIUM PHOSPHATE,
REMARK 210 150 MM SODIUM CHLORIDE, 0.1 MM
REMARK 210 EDTA, 0.1 MM PMSF, 5 MM DTT, 500
REMARK 210 UM [U-13C; U-15N] MED15 ABD2,
REMARK 210 100% D2O; 20 MM SODIUM PHOSPHATE,
REMARK 210 150 MM SODIUM CHLORIDE, 0.1 MM
REMARK 210 EDTA, 0.1 MM PMSF, 5 MM DTT, 10 %
REMARK 210 C12E6/HEXANOL, 2.6 MM [U-15N]
REMARK 210 MED15 ABD2, 90% H2O/10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 2D 1H-15N HSQC; 3D 1H-15N TOCSY;
REMARK 210 3D HBHA(CO)NH; 3D H(CCO)NH; 3D
REMARK 210 C(CO)NH; 3D 1H-15N NOESY; 3D
REMARK 210 HNCO; 3D HNCA; 3D HN(CO)CA; 3D
REMARK 210 CBCA(CO)NH; 3D HNCACB; 2D 1H-13C
REMARK 210 HSQC; 3D 1H-13C NOESY AROMATIC;
REMARK 210 3D HCCH-TOCSY; 3D HCCH-COSY; 2D
REMARK 210 1H-15N HSQC IPAP
REMARK 210 SPECTROMETER FIELD STRENGTH : 500 MHZ; 600 MHZ; 800 MHZ
REMARK 210 SPECTROMETER MODEL : AVANCE III
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NMRVIEW, NMRPIPE, TALOS, TOPSPIN
REMARK 210 METHOD USED : SIMULATED ANNEALING
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 200
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LOWEST
REMARK 210 ENERGY
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 LEU A 324 78.75 61.67
REMARK 500 1 SER A 326 -150.36 -165.39
REMARK 500 2 GLN A 283 -86.95 -103.05
REMARK 500 3 SER A 285 115.96 -161.27
REMARK 500 3 ALA A 361 -90.19 -159.70
REMARK 500 4 GLN A 286 -64.88 -104.86
REMARK 500 4 GLN A 323 154.71 72.87
REMARK 500 5 LEU A 280 62.83 61.05
REMARK 500 5 LEU A 324 -159.32 -127.26
REMARK 500 5 ALA A 361 -96.71 -164.31
REMARK 500 6 SER A 284 67.64 68.11
REMARK 500 6 THR A 288 -30.02 -130.33
REMARK 500 6 VAL A 355 -85.75 -106.35
REMARK 500 6 ASN A 360 30.01 -145.25
REMARK 500 7 GLN A 323 149.41 75.12
REMARK 500 8 ASN A 357 44.08 -145.41
REMARK 500 9 ASN A 291 -66.14 -171.51
REMARK 500 9 GLN A 323 68.14 61.40
REMARK 500 10 GLN A 286 -162.60 61.77
REMARK 500 10 PRO A 290 49.61 -92.35
REMARK 500 10 GLN A 323 159.02 68.98
REMARK 500 10 LEU A 324 11.86 -151.99
REMARK 500 11 GLN A 283 107.28 -168.43
REMARK 500 12 ASN A 358 -160.19 61.69
REMARK 500 13 LEU A 280 -154.00 61.75
REMARK 500 13 SER A 284 -79.62 -158.25
REMARK 500 13 SER A 326 -154.52 -165.76
REMARK 500 14 LEU A 324 118.35 -162.18
REMARK 500 14 ASN A 362 -139.44 60.23
REMARK 500 15 LEU A 324 -164.05 -103.95
REMARK 500 16 LEU A 324 118.15 -171.04
REMARK 500 16 SER A 326 -115.94 -147.37
REMARK 500 16 ALA A 361 66.12 63.59
REMARK 500 17 GLN A 323 -53.27 75.14
REMARK 500 17 LEU A 324 -116.26 63.18
REMARK 500 17 VAL A 355 -79.75 -98.92
REMARK 500 18 GLN A 281 -138.81 60.21
REMARK 500 18 GLN A 283 -80.99 -138.24
REMARK 500 18 LEU A 324 -82.66 -98.51
REMARK 500 18 SER A 326 -104.23 -91.45
REMARK 500 19 GLN A 323 77.91 65.85
REMARK 500 20 VAL A 289 72.22 58.58
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 30330 RELATED DB: BMRB
REMARK 900 SOLUTION STRUCTURE OF YEAST MED15 ABD2 RESIDUES 277-368
DBREF 6ALY A 277 368 UNP P19659 MED15_YEAST 277 368
SEQRES 1 A 92 ASN ASN PRO LEU GLN GLN GLN SER SER GLN ASN THR VAL
SEQRES 2 A 92 PRO ASN VAL LEU ASN GLN ILE ASN GLN ILE PHE SER PRO
SEQRES 3 A 92 GLU GLU GLN ARG SER LEU LEU GLN GLU ALA ILE GLU THR
SEQRES 4 A 92 CYS LYS ASN PHE GLU LYS THR GLN LEU GLY SER THR MET
SEQRES 5 A 92 THR GLU PRO VAL LYS GLN SER PHE ILE ARG LYS TYR ILE
SEQRES 6 A 92 ASN GLN LYS ALA LEU ARG LYS ILE GLN ALA LEU ARG ASP
SEQRES 7 A 92 VAL LYS ASN ASN ASN ASN ALA ASN ASN ASN GLY SER ASN
SEQRES 8 A 92 LEU
HELIX 1 AA1 GLN A 295 PHE A 300 1 6
HELIX 2 AA2 SER A 301 GLN A 323 1 23
HELIX 3 AA3 THR A 329 ASP A 354 1 26
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
