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Database: PDB
Entry: 6B5H
LinkDB: 6B5H
Original site: 6B5H 
HEADER    OXIDOREDUCTASE/OXIDOREDUCTASE INHIBITOR 29-SEP-17   6B5H              
TITLE     ALDH1A2 LIGANDED WITH NAD AND 1-(4-CYANOPHENYL)-N-(3-FLUOROPHENYL)-3- 
TITLE    2 [4-(METHYLSULFONYL)PHENYL]-1H-PYRAZOLE-4-CARBOXAMIDE (COMPOUND CM121)
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RETINAL DEHYDROGENASE 2;                                   
COMPND   3 CHAIN: A, B, C, D;                                                   
COMPND   4 FRAGMENT: UNP RESIDUES 26-518;                                       
COMPND   5 SYNONYM: RALDH2, ALDEHYDE DEHYDROGENASE FAMILY 1 MEMBER A2,          
COMPND   6 RETINALDEHYDE-SPECIFIC DEHYDROGENASE TYPE 2, RALDH(II);              
COMPND   7 EC: 1.2.1.36;                                                        
COMPND   8 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ALDH1A2, RALDH2;                                               
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21STAR(DE3);                             
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28                                     
KEYWDS    RETINOIC ACID SIGNALING, MALE CONTRACEPTION, DRUG DISCOVERY, DRUG     
KEYWDS   2 DEVELOPMENT, OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR COMPLEX         
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Y.CHEN,J.-Y.ZHU,E.SCHONBRUNN                                          
REVDAT   4   11-DEC-19 6B5H    1       REMARK                                   
REVDAT   3   28-MAR-18 6B5H    1       JRNL                                     
REVDAT   2   17-JAN-18 6B5H    1       JRNL                                     
REVDAT   1   10-JAN-18 6B5H    0                                                
JRNL        AUTH   Y.CHEN,J.Y.ZHU,K.H.HONG,D.C.MIKLES,G.I.GEORG,A.S.GOLDSTEIN,  
JRNL        AUTH 2 J.K.AMORY,E.SCHONBRUNN                                       
JRNL        TITL   STRUCTURAL BASIS OF ALDH1A2 INHIBITION BY IRREVERSIBLE AND   
JRNL        TITL 2 REVERSIBLE SMALL MOLECULE INHIBITORS.                        
JRNL        REF    ACS CHEM. BIOL.               V.  13   582 2018              
JRNL        REFN                   ESSN 1554-8937                               
JRNL        PMID   29240402                                                     
JRNL        DOI    10.1021/ACSCHEMBIO.7B00685                                   
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.30 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.11.1-2575_2575                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.30                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 72.82                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 88755                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.207                           
REMARK   3   R VALUE            (WORKING SET) : 0.206                           
REMARK   3   FREE R VALUE                     : 0.264                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 2.000                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1775                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 72.8549 -  5.4075    0.99     7015   143  0.1884 0.2218        
REMARK   3     2  5.4075 -  4.2922    1.00     6795   139  0.1693 0.2224        
REMARK   3     3  4.2922 -  3.7497    1.00     6746   138  0.1859 0.2490        
REMARK   3     4  3.7497 -  3.4068    1.00     6706   137  0.2012 0.2499        
REMARK   3     5  3.4068 -  3.1626    1.00     6688   136  0.2139 0.2806        
REMARK   3     6  3.1626 -  2.9762    1.00     6686   136  0.2260 0.2944        
REMARK   3     7  2.9762 -  2.8271    1.00     6627   136  0.2253 0.2830        
REMARK   3     8  2.8271 -  2.7041    1.00     6656   136  0.2408 0.3120        
REMARK   3     9  2.7041 -  2.5999    1.00     6617   135  0.2415 0.3181        
REMARK   3    10  2.5999 -  2.5102    1.00     6606   134  0.2398 0.2994        
REMARK   3    11  2.5102 -  2.4317    1.00     6623   136  0.2420 0.3388        
REMARK   3    12  2.4317 -  2.3622    1.00     6589   134  0.2498 0.3345        
REMARK   3    13  2.3622 -  2.3000    1.00     6626   135  0.2591 0.3196        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.320            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.210           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 37.92                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 42.33                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.002          15907                                  
REMARK   3   ANGLE     :  0.541          21572                                  
REMARK   3   CHIRALITY :  0.041           2336                                  
REMARK   3   PLANARITY :  0.003           2808                                  
REMARK   3   DIHEDRAL  :  6.099           9364                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6B5H COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 02-OCT-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000229410.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 08-NOV-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 22-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.00                               
REMARK 200  MONOCHROMATOR                  : ROSENBAUM-ROCK DOUBLE CRYSTAL      
REMARK 200                                   SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 300 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : XSCALE                             
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 88789                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.300                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 72.818                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY                : 7.426                              
REMARK 200  R MERGE                    (I) : 0.07900                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 17.7900                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.40                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.56                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.64200                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 3.760                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: NULL                                                  
REMARK 200 STARTING MODEL: PDB ENTRY 6ALJ                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 46.24                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.29                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 40 MG/ML ALDH1A2, 0.2 M SODIUM CITRATE   
REMARK 280  TRIBASIC DEHYDRATE, 25% W/V PEG3350, 1.3 MM CM121, 10% DMSO,        
REMARK 280  VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       42.42500            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       82.37500            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       70.93000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       82.37500            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       42.42500            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       70.93000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 24650 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 58690 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -104.0 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     LEU A    26                                                      
REMARK 465     LEU B    26                                                      
REMARK 465     LEU C    26                                                      
REMARK 465     LEU D    26                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   NZ   LYS C   210     O2B  NAD C   601              1.30            
REMARK 500   O    GLU D   286     N7N  NAD D   601              1.30            
REMARK 500   CE   LYS D   210     O2B  NAD D   601              1.38            
REMARK 500   C    GLU D   286     N7N  NAD D   601              1.95            
REMARK 500   O    THR B   321     OH   TYR B   474              2.17            
REMARK 500   OE2  GLU B   386     OG   SER B   405              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    PRO A  31      -76.69    -58.11                                   
REMARK 500    ASP A  73     -157.56   -133.51                                   
REMARK 500    ASP A 116       36.52    -98.24                                   
REMARK 500    THR A 245      -70.40    -97.19                                   
REMARK 500    SER A 278      -96.02    -91.69                                   
REMARK 500    ASP A 298       36.30    -85.91                                   
REMARK 500    ASP A 300       98.35    -57.73                                   
REMARK 500    GLN A 318       54.87   -106.07                                   
REMARK 500    PHE A 419       63.37    -67.93                                   
REMARK 500    TYR A 474      105.90    179.17                                   
REMARK 500    LYS A 487     -131.15     52.29                                   
REMARK 500    MET A 495      161.44     65.20                                   
REMARK 500    LYS A 516      123.34     60.66                                   
REMARK 500    PRO B  31      -95.61    -61.77                                   
REMARK 500    THR B  62      -49.04   -131.70                                   
REMARK 500    ASP B  73     -163.76   -128.39                                   
REMARK 500    PRO B 185     -157.56    -88.94                                   
REMARK 500    THR B 245      -73.88    -97.83                                   
REMARK 500    SER B 278     -102.82    -88.20                                   
REMARK 500    SER B 324      -88.28     49.69                                   
REMARK 500    ARG B 347      102.37    -56.67                                   
REMARK 500    VAL B 348       98.82    -67.58                                   
REMARK 500    ASN B 369      -61.66    -91.94                                   
REMARK 500    LYS B 384       82.70    -62.18                                   
REMARK 500    GLU B 386      -98.80    -64.21                                   
REMARK 500    LYS B 390      129.02     86.36                                   
REMARK 500    ARG B 394       22.81    -64.69                                   
REMARK 500    LYS B 395       61.31    -66.19                                   
REMARK 500    PRO B 421       61.38    -68.84                                   
REMARK 500    ASN B 440       77.52    -62.99                                   
REMARK 500    LYS B 487     -133.28     48.97                                   
REMARK 500    MET B 488       47.64    -85.78                                   
REMARK 500    SER B 489       28.58   -162.58                                   
REMARK 500    ASN B 491      104.99     -8.43                                   
REMARK 500    MET B 495      163.73     70.34                                   
REMARK 500    CYS C  67     -168.65   -161.68                                   
REMARK 500    ASP C 116       38.82    -93.51                                   
REMARK 500    PRO C 244        0.34    -64.65                                   
REMARK 500    THR C 245      -75.16   -112.86                                   
REMARK 500    SER C 278      -76.70   -117.94                                   
REMARK 500    LEU C 287     -169.67   -115.45                                   
REMARK 500    GLN C 318       43.60    -99.63                                   
REMARK 500    GLU C 381     -164.31   -106.71                                   
REMARK 500    ARG C 394      -52.57   -127.11                                   
REMARK 500    PHE C 397       56.96   -115.25                                   
REMARK 500    SER C 441      140.46   -172.46                                   
REMARK 500    GLN C 465       66.26   -103.96                                   
REMARK 500    TYR C 474     -113.21   -131.68                                   
REMARK 500    ASN C 475       45.32    -57.76                                   
REMARK 500    GLN C 480     -166.65    -72.58                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      72 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue NAD A 601                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CU4 A 602                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue NAD B 601                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CU4 B 602                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CU4 C 602                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CU4 D 602                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 6ALJ   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 6B5G   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 6B5I   RELATED DB: PDB                                   
DBREF  6B5H A   26   518  UNP    O94788   AL1A2_HUMAN     26    518             
DBREF  6B5H B   26   518  UNP    O94788   AL1A2_HUMAN     26    518             
DBREF  6B5H C   26   518  UNP    O94788   AL1A2_HUMAN     26    518             
DBREF  6B5H D   26   518  UNP    O94788   AL1A2_HUMAN     26    518             
SEQRES   1 A  493  LEU PRO SER PRO THR PRO ASN LEU GLU ILE LYS TYR THR          
SEQRES   2 A  493  LYS ILE PHE ILE ASN ASN GLU TRP GLN ASN SER GLU SER          
SEQRES   3 A  493  GLY ARG VAL PHE PRO VAL TYR ASN PRO ALA THR GLY GLU          
SEQRES   4 A  493  GLN VAL CYS GLU VAL GLN GLU ALA ASP LYS ALA ASP ILE          
SEQRES   5 A  493  ASP LYS ALA VAL GLN ALA ALA ARG LEU ALA PHE SER LEU          
SEQRES   6 A  493  GLY SER VAL TRP ARG ARG MET ASP ALA SER GLU ARG GLY          
SEQRES   7 A  493  ARG LEU LEU ASP LYS LEU ALA ASP LEU VAL GLU ARG ASP          
SEQRES   8 A  493  ARG ALA VAL LEU ALA THR MET GLU SER LEU ASN GLY GLY          
SEQRES   9 A  493  LYS PRO PHE LEU GLN ALA PHE TYR VAL ASP LEU GLN GLY          
SEQRES  10 A  493  VAL ILE LYS THR PHE ARG TYR TYR ALA GLY TRP ALA ASP          
SEQRES  11 A  493  LYS ILE HIS GLY MET THR ILE PRO VAL ASP GLY ASP TYR          
SEQRES  12 A  493  PHE THR PHE THR ARG HIS GLU PRO ILE GLY VAL CYS GLY          
SEQRES  13 A  493  GLN ILE ILE PRO TRP ASN PHE PRO LEU LEU MET PHE ALA          
SEQRES  14 A  493  TRP LYS ILE ALA PRO ALA LEU CYS CYS GLY ASN THR VAL          
SEQRES  15 A  493  VAL ILE LYS PRO ALA GLU GLN THR PRO LEU SER ALA LEU          
SEQRES  16 A  493  TYR MET GLY ALA LEU ILE LYS GLU ALA GLY PHE PRO PRO          
SEQRES  17 A  493  GLY VAL ILE ASN ILE LEU PRO GLY TYR GLY PRO THR ALA          
SEQRES  18 A  493  GLY ALA ALA ILE ALA SER HIS ILE GLY ILE ASP LYS ILE          
SEQRES  19 A  493  ALA PHE THR GLY SER THR GLU VAL GLY LYS LEU ILE GLN          
SEQRES  20 A  493  GLU ALA ALA GLY ARG SER ASN LEU LYS ARG VAL THR LEU          
SEQRES  21 A  493  GLU LEU GLY GLY LYS SER PRO ASN ILE ILE PHE ALA ASP          
SEQRES  22 A  493  ALA ASP LEU ASP TYR ALA VAL GLU GLN ALA HIS GLN GLY          
SEQRES  23 A  493  VAL PHE PHE ASN GLN GLY GLN CYS CYS THR ALA GLY SER          
SEQRES  24 A  493  ARG ILE PHE VAL GLU GLU SER ILE TYR GLU GLU PHE VAL          
SEQRES  25 A  493  ARG ARG SER VAL GLU ARG ALA LYS ARG ARG VAL VAL GLY          
SEQRES  26 A  493  SER PRO PHE ASP PRO THR THR GLU GLN GLY PRO GLN ILE          
SEQRES  27 A  493  ASP LYS LYS GLN TYR ASN LYS ILE LEU GLU LEU ILE GLN          
SEQRES  28 A  493  SER GLY VAL ALA GLU GLY ALA LYS LEU GLU CYS GLY GLY          
SEQRES  29 A  493  LYS GLY LEU GLY ARG LYS GLY PHE PHE ILE GLU PRO THR          
SEQRES  30 A  493  VAL PHE SER ASN VAL THR ASP ASP MET ARG ILE ALA LYS          
SEQRES  31 A  493  GLU GLU ILE PHE GLY PRO VAL GLN GLU ILE LEU ARG PHE          
SEQRES  32 A  493  LYS THR MET ASP GLU VAL ILE GLU ARG ALA ASN ASN SER          
SEQRES  33 A  493  ASP PHE GLY LEU VAL ALA ALA VAL PHE THR ASN ASP ILE          
SEQRES  34 A  493  ASN LYS ALA LEU THR VAL SER SER ALA MET GLN ALA GLY          
SEQRES  35 A  493  THR VAL TRP ILE ASN CYS TYR ASN ALA LEU ASN ALA GLN          
SEQRES  36 A  493  SER PRO PHE GLY GLY PHE LYS MET SER GLY ASN GLY ARG          
SEQRES  37 A  493  GLU MET GLY GLU PHE GLY LEU ARG GLU TYR SER GLU VAL          
SEQRES  38 A  493  LYS THR VAL THR VAL LYS ILE PRO GLN LYS ASN SER              
SEQRES   1 B  493  LEU PRO SER PRO THR PRO ASN LEU GLU ILE LYS TYR THR          
SEQRES   2 B  493  LYS ILE PHE ILE ASN ASN GLU TRP GLN ASN SER GLU SER          
SEQRES   3 B  493  GLY ARG VAL PHE PRO VAL TYR ASN PRO ALA THR GLY GLU          
SEQRES   4 B  493  GLN VAL CYS GLU VAL GLN GLU ALA ASP LYS ALA ASP ILE          
SEQRES   5 B  493  ASP LYS ALA VAL GLN ALA ALA ARG LEU ALA PHE SER LEU          
SEQRES   6 B  493  GLY SER VAL TRP ARG ARG MET ASP ALA SER GLU ARG GLY          
SEQRES   7 B  493  ARG LEU LEU ASP LYS LEU ALA ASP LEU VAL GLU ARG ASP          
SEQRES   8 B  493  ARG ALA VAL LEU ALA THR MET GLU SER LEU ASN GLY GLY          
SEQRES   9 B  493  LYS PRO PHE LEU GLN ALA PHE TYR VAL ASP LEU GLN GLY          
SEQRES  10 B  493  VAL ILE LYS THR PHE ARG TYR TYR ALA GLY TRP ALA ASP          
SEQRES  11 B  493  LYS ILE HIS GLY MET THR ILE PRO VAL ASP GLY ASP TYR          
SEQRES  12 B  493  PHE THR PHE THR ARG HIS GLU PRO ILE GLY VAL CYS GLY          
SEQRES  13 B  493  GLN ILE ILE PRO TRP ASN PHE PRO LEU LEU MET PHE ALA          
SEQRES  14 B  493  TRP LYS ILE ALA PRO ALA LEU CYS CYS GLY ASN THR VAL          
SEQRES  15 B  493  VAL ILE LYS PRO ALA GLU GLN THR PRO LEU SER ALA LEU          
SEQRES  16 B  493  TYR MET GLY ALA LEU ILE LYS GLU ALA GLY PHE PRO PRO          
SEQRES  17 B  493  GLY VAL ILE ASN ILE LEU PRO GLY TYR GLY PRO THR ALA          
SEQRES  18 B  493  GLY ALA ALA ILE ALA SER HIS ILE GLY ILE ASP LYS ILE          
SEQRES  19 B  493  ALA PHE THR GLY SER THR GLU VAL GLY LYS LEU ILE GLN          
SEQRES  20 B  493  GLU ALA ALA GLY ARG SER ASN LEU LYS ARG VAL THR LEU          
SEQRES  21 B  493  GLU LEU GLY GLY LYS SER PRO ASN ILE ILE PHE ALA ASP          
SEQRES  22 B  493  ALA ASP LEU ASP TYR ALA VAL GLU GLN ALA HIS GLN GLY          
SEQRES  23 B  493  VAL PHE PHE ASN GLN GLY GLN CYS CYS THR ALA GLY SER          
SEQRES  24 B  493  ARG ILE PHE VAL GLU GLU SER ILE TYR GLU GLU PHE VAL          
SEQRES  25 B  493  ARG ARG SER VAL GLU ARG ALA LYS ARG ARG VAL VAL GLY          
SEQRES  26 B  493  SER PRO PHE ASP PRO THR THR GLU GLN GLY PRO GLN ILE          
SEQRES  27 B  493  ASP LYS LYS GLN TYR ASN LYS ILE LEU GLU LEU ILE GLN          
SEQRES  28 B  493  SER GLY VAL ALA GLU GLY ALA LYS LEU GLU CYS GLY GLY          
SEQRES  29 B  493  LYS GLY LEU GLY ARG LYS GLY PHE PHE ILE GLU PRO THR          
SEQRES  30 B  493  VAL PHE SER ASN VAL THR ASP ASP MET ARG ILE ALA LYS          
SEQRES  31 B  493  GLU GLU ILE PHE GLY PRO VAL GLN GLU ILE LEU ARG PHE          
SEQRES  32 B  493  LYS THR MET ASP GLU VAL ILE GLU ARG ALA ASN ASN SER          
SEQRES  33 B  493  ASP PHE GLY LEU VAL ALA ALA VAL PHE THR ASN ASP ILE          
SEQRES  34 B  493  ASN LYS ALA LEU THR VAL SER SER ALA MET GLN ALA GLY          
SEQRES  35 B  493  THR VAL TRP ILE ASN CYS TYR ASN ALA LEU ASN ALA GLN          
SEQRES  36 B  493  SER PRO PHE GLY GLY PHE LYS MET SER GLY ASN GLY ARG          
SEQRES  37 B  493  GLU MET GLY GLU PHE GLY LEU ARG GLU TYR SER GLU VAL          
SEQRES  38 B  493  LYS THR VAL THR VAL LYS ILE PRO GLN LYS ASN SER              
SEQRES   1 C  493  LEU PRO SER PRO THR PRO ASN LEU GLU ILE LYS TYR THR          
SEQRES   2 C  493  LYS ILE PHE ILE ASN ASN GLU TRP GLN ASN SER GLU SER          
SEQRES   3 C  493  GLY ARG VAL PHE PRO VAL TYR ASN PRO ALA THR GLY GLU          
SEQRES   4 C  493  GLN VAL CYS GLU VAL GLN GLU ALA ASP LYS ALA ASP ILE          
SEQRES   5 C  493  ASP LYS ALA VAL GLN ALA ALA ARG LEU ALA PHE SER LEU          
SEQRES   6 C  493  GLY SER VAL TRP ARG ARG MET ASP ALA SER GLU ARG GLY          
SEQRES   7 C  493  ARG LEU LEU ASP LYS LEU ALA ASP LEU VAL GLU ARG ASP          
SEQRES   8 C  493  ARG ALA VAL LEU ALA THR MET GLU SER LEU ASN GLY GLY          
SEQRES   9 C  493  LYS PRO PHE LEU GLN ALA PHE TYR VAL ASP LEU GLN GLY          
SEQRES  10 C  493  VAL ILE LYS THR PHE ARG TYR TYR ALA GLY TRP ALA ASP          
SEQRES  11 C  493  LYS ILE HIS GLY MET THR ILE PRO VAL ASP GLY ASP TYR          
SEQRES  12 C  493  PHE THR PHE THR ARG HIS GLU PRO ILE GLY VAL CYS GLY          
SEQRES  13 C  493  GLN ILE ILE PRO TRP ASN PHE PRO LEU LEU MET PHE ALA          
SEQRES  14 C  493  TRP LYS ILE ALA PRO ALA LEU CYS CYS GLY ASN THR VAL          
SEQRES  15 C  493  VAL ILE LYS PRO ALA GLU GLN THR PRO LEU SER ALA LEU          
SEQRES  16 C  493  TYR MET GLY ALA LEU ILE LYS GLU ALA GLY PHE PRO PRO          
SEQRES  17 C  493  GLY VAL ILE ASN ILE LEU PRO GLY TYR GLY PRO THR ALA          
SEQRES  18 C  493  GLY ALA ALA ILE ALA SER HIS ILE GLY ILE ASP LYS ILE          
SEQRES  19 C  493  ALA PHE THR GLY SER THR GLU VAL GLY LYS LEU ILE GLN          
SEQRES  20 C  493  GLU ALA ALA GLY ARG SER ASN LEU LYS ARG VAL THR LEU          
SEQRES  21 C  493  GLU LEU GLY GLY LYS SER PRO ASN ILE ILE PHE ALA ASP          
SEQRES  22 C  493  ALA ASP LEU ASP TYR ALA VAL GLU GLN ALA HIS GLN GLY          
SEQRES  23 C  493  VAL PHE PHE ASN GLN GLY GLN CYS CYS THR ALA GLY SER          
SEQRES  24 C  493  ARG ILE PHE VAL GLU GLU SER ILE TYR GLU GLU PHE VAL          
SEQRES  25 C  493  ARG ARG SER VAL GLU ARG ALA LYS ARG ARG VAL VAL GLY          
SEQRES  26 C  493  SER PRO PHE ASP PRO THR THR GLU GLN GLY PRO GLN ILE          
SEQRES  27 C  493  ASP LYS LYS GLN TYR ASN LYS ILE LEU GLU LEU ILE GLN          
SEQRES  28 C  493  SER GLY VAL ALA GLU GLY ALA LYS LEU GLU CYS GLY GLY          
SEQRES  29 C  493  LYS GLY LEU GLY ARG LYS GLY PHE PHE ILE GLU PRO THR          
SEQRES  30 C  493  VAL PHE SER ASN VAL THR ASP ASP MET ARG ILE ALA LYS          
SEQRES  31 C  493  GLU GLU ILE PHE GLY PRO VAL GLN GLU ILE LEU ARG PHE          
SEQRES  32 C  493  LYS THR MET ASP GLU VAL ILE GLU ARG ALA ASN ASN SER          
SEQRES  33 C  493  ASP PHE GLY LEU VAL ALA ALA VAL PHE THR ASN ASP ILE          
SEQRES  34 C  493  ASN LYS ALA LEU THR VAL SER SER ALA MET GLN ALA GLY          
SEQRES  35 C  493  THR VAL TRP ILE ASN CYS TYR ASN ALA LEU ASN ALA GLN          
SEQRES  36 C  493  SER PRO PHE GLY GLY PHE LYS MET SER GLY ASN GLY ARG          
SEQRES  37 C  493  GLU MET GLY GLU PHE GLY LEU ARG GLU TYR SER GLU VAL          
SEQRES  38 C  493  LYS THR VAL THR VAL LYS ILE PRO GLN LYS ASN SER              
SEQRES   1 D  493  LEU PRO SER PRO THR PRO ASN LEU GLU ILE LYS TYR THR          
SEQRES   2 D  493  LYS ILE PHE ILE ASN ASN GLU TRP GLN ASN SER GLU SER          
SEQRES   3 D  493  GLY ARG VAL PHE PRO VAL TYR ASN PRO ALA THR GLY GLU          
SEQRES   4 D  493  GLN VAL CYS GLU VAL GLN GLU ALA ASP LYS ALA ASP ILE          
SEQRES   5 D  493  ASP LYS ALA VAL GLN ALA ALA ARG LEU ALA PHE SER LEU          
SEQRES   6 D  493  GLY SER VAL TRP ARG ARG MET ASP ALA SER GLU ARG GLY          
SEQRES   7 D  493  ARG LEU LEU ASP LYS LEU ALA ASP LEU VAL GLU ARG ASP          
SEQRES   8 D  493  ARG ALA VAL LEU ALA THR MET GLU SER LEU ASN GLY GLY          
SEQRES   9 D  493  LYS PRO PHE LEU GLN ALA PHE TYR VAL ASP LEU GLN GLY          
SEQRES  10 D  493  VAL ILE LYS THR PHE ARG TYR TYR ALA GLY TRP ALA ASP          
SEQRES  11 D  493  LYS ILE HIS GLY MET THR ILE PRO VAL ASP GLY ASP TYR          
SEQRES  12 D  493  PHE THR PHE THR ARG HIS GLU PRO ILE GLY VAL CYS GLY          
SEQRES  13 D  493  GLN ILE ILE PRO TRP ASN PHE PRO LEU LEU MET PHE ALA          
SEQRES  14 D  493  TRP LYS ILE ALA PRO ALA LEU CYS CYS GLY ASN THR VAL          
SEQRES  15 D  493  VAL ILE LYS PRO ALA GLU GLN THR PRO LEU SER ALA LEU          
SEQRES  16 D  493  TYR MET GLY ALA LEU ILE LYS GLU ALA GLY PHE PRO PRO          
SEQRES  17 D  493  GLY VAL ILE ASN ILE LEU PRO GLY TYR GLY PRO THR ALA          
SEQRES  18 D  493  GLY ALA ALA ILE ALA SER HIS ILE GLY ILE ASP LYS ILE          
SEQRES  19 D  493  ALA PHE THR GLY SER THR GLU VAL GLY LYS LEU ILE GLN          
SEQRES  20 D  493  GLU ALA ALA GLY ARG SER ASN LEU LYS ARG VAL THR LEU          
SEQRES  21 D  493  GLU LEU GLY GLY LYS SER PRO ASN ILE ILE PHE ALA ASP          
SEQRES  22 D  493  ALA ASP LEU ASP TYR ALA VAL GLU GLN ALA HIS GLN GLY          
SEQRES  23 D  493  VAL PHE PHE ASN GLN GLY GLN CYS CYS THR ALA GLY SER          
SEQRES  24 D  493  ARG ILE PHE VAL GLU GLU SER ILE TYR GLU GLU PHE VAL          
SEQRES  25 D  493  ARG ARG SER VAL GLU ARG ALA LYS ARG ARG VAL VAL GLY          
SEQRES  26 D  493  SER PRO PHE ASP PRO THR THR GLU GLN GLY PRO GLN ILE          
SEQRES  27 D  493  ASP LYS LYS GLN TYR ASN LYS ILE LEU GLU LEU ILE GLN          
SEQRES  28 D  493  SER GLY VAL ALA GLU GLY ALA LYS LEU GLU CYS GLY GLY          
SEQRES  29 D  493  LYS GLY LEU GLY ARG LYS GLY PHE PHE ILE GLU PRO THR          
SEQRES  30 D  493  VAL PHE SER ASN VAL THR ASP ASP MET ARG ILE ALA LYS          
SEQRES  31 D  493  GLU GLU ILE PHE GLY PRO VAL GLN GLU ILE LEU ARG PHE          
SEQRES  32 D  493  LYS THR MET ASP GLU VAL ILE GLU ARG ALA ASN ASN SER          
SEQRES  33 D  493  ASP PHE GLY LEU VAL ALA ALA VAL PHE THR ASN ASP ILE          
SEQRES  34 D  493  ASN LYS ALA LEU THR VAL SER SER ALA MET GLN ALA GLY          
SEQRES  35 D  493  THR VAL TRP ILE ASN CYS TYR ASN ALA LEU ASN ALA GLN          
SEQRES  36 D  493  SER PRO PHE GLY GLY PHE LYS MET SER GLY ASN GLY ARG          
SEQRES  37 D  493  GLU MET GLY GLU PHE GLY LEU ARG GLU TYR SER GLU VAL          
SEQRES  38 D  493  LYS THR VAL THR VAL LYS ILE PRO GLN LYS ASN SER              
HET    NAD  A 601      44                                                       
HET    CU4  A 602      33                                                       
HET    NAD  B 601      44                                                       
HET    CU4  B 602      33                                                       
HET    NAD  C 601      44                                                       
HET    CU4  C 602      33                                                       
HET    NAD  D 601      44                                                       
HET    CU4  D 602      33                                                       
HETNAM     NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE                                
HETNAM     CU4 1-(4-CYANOPHENYL)-N-(3-FLUOROPHENYL)-3-[4-                       
HETNAM   2 CU4  (METHYLSULFONYL)PHENYL]-1H-PYRAZOLE-4-CARBOXAMIDE               
FORMUL   5  NAD    4(C21 H27 N7 O14 P2)                                         
FORMUL   6  CU4    4(C24 H17 F N4 O3 S)                                         
FORMUL  13  HOH   *382(H2 O)                                                    
HELIX    1 AA1 ASP A   73  ALA A   87  1                                  15    
HELIX    2 AA2 SER A   92  MET A   97  1                                   6    
HELIX    3 AA3 ASP A   98  ASP A  116  1                                  19    
HELIX    4 AA4 ASP A  116  GLY A  129  1                                  14    
HELIX    5 AA5 PRO A  131  VAL A  138  1                                   8    
HELIX    6 AA6 VAL A  138  GLY A  152  1                                  15    
HELIX    7 AA7 TRP A  153  ILE A  157  5                                   5    
HELIX    8 AA8 PHE A  188  CYS A  203  1                                  16    
HELIX    9 AA9 PRO A  216  GLY A  230  1                                  15    
HELIX   10 AB1 THR A  245  HIS A  253  1                                   9    
HELIX   11 AB2 SER A  264  SER A  278  1                                  15    
HELIX   12 AB3 ASP A  300  PHE A  314  1                                  15    
HELIX   13 AB4 ASN A  315  GLN A  318  5                                   4    
HELIX   14 AB5 ILE A  332  ARG A  346  1                                  15    
HELIX   15 AB6 ASP A  364  GLU A  381  1                                  18    
HELIX   16 AB7 MET A  411  GLU A  416  1                                   6    
HELIX   17 AB8 THR A  430  ASN A  440  1                                  11    
HELIX   18 AB9 ASP A  453  MET A  464  1                                  12    
HELIX   19 AC1 GLY A  496  GLU A  502  5                                   7    
HELIX   20 AC2 ASP B   73  SER B   89  1                                  17    
HELIX   21 AC3 SER B   92  MET B   97  1                                   6    
HELIX   22 AC4 ASP B   98  ASP B  116  1                                  19    
HELIX   23 AC5 ASP B  116  GLY B  129  1                                  14    
HELIX   24 AC6 PRO B  131  VAL B  138  1                                   8    
HELIX   25 AC7 VAL B  138  ALA B  154  1                                  17    
HELIX   26 AC8 ASP B  155  ILE B  157  5                                   3    
HELIX   27 AC9 PHE B  188  CYS B  203  1                                  16    
HELIX   28 AD1 PRO B  216  GLY B  230  1                                  15    
HELIX   29 AD2 TYR B  242  SER B  252  1                                  11    
HELIX   30 AD3 SER B  264  SER B  278  1                                  15    
HELIX   31 AD4 ASP B  300  PHE B  314  1                                  15    
HELIX   32 AD5 ASN B  315  GLN B  318  5                                   4    
HELIX   33 AD6 ILE B  332  LYS B  345  1                                  14    
HELIX   34 AD7 ASP B  364  GLU B  381  1                                  18    
HELIX   35 AD8 MET B  411  LYS B  415  5                                   5    
HELIX   36 AD9 THR B  430  ASN B  440  1                                  11    
HELIX   37 AE1 ASP B  453  MET B  464  1                                  12    
HELIX   38 AE2 PHE B  486  GLY B  490  5                                   5    
HELIX   39 AE3 GLU B  497  GLU B  502  1                                   6    
HELIX   40 AE4 ASP C   73  SER C   89  1                                  17    
HELIX   41 AE5 SER C   92  MET C   97  1                                   6    
HELIX   42 AE6 ASP C   98  ASP C  116  1                                  19    
HELIX   43 AE7 ASP C  116  GLY C  129  1                                  14    
HELIX   44 AE8 PRO C  131  VAL C  138  1                                   8    
HELIX   45 AE9 VAL C  138  GLY C  152  1                                  15    
HELIX   46 AF1 TRP C  153  ILE C  157  5                                   5    
HELIX   47 AF2 PHE C  188  CYS C  203  1                                  16    
HELIX   48 AF3 PRO C  216  GLY C  230  1                                  15    
HELIX   49 AF4 TYR C  242  HIS C  253  1                                  12    
HELIX   50 AF5 SER C  264  SER C  278  1                                  15    
HELIX   51 AF6 ASP C  300  PHE C  314  1                                  15    
HELIX   52 AF7 ASN C  315  GLN C  318  5                                   4    
HELIX   53 AF8 ILE C  332  ARG C  347  1                                  16    
HELIX   54 AF9 ASP C  364  GLU C  381  1                                  18    
HELIX   55 AG1 MET C  411  GLU C  416  1                                   6    
HELIX   56 AG2 THR C  430  ASN C  440  1                                  11    
HELIX   57 AG3 ASP C  453  MET C  464  1                                  12    
HELIX   58 AG4 PHE C  486  MET C  488  5                                   3    
HELIX   59 AG5 GLU C  497  GLU C  502  1                                   6    
HELIX   60 AG6 ASP D   73  PHE D   88  1                                  16    
HELIX   61 AG7 SER D   92  MET D   97  1                                   6    
HELIX   62 AG8 ASP D   98  ASP D  116  1                                  19    
HELIX   63 AG9 ASP D  116  GLY D  129  1                                  14    
HELIX   64 AH1 PRO D  131  VAL D  138  1                                   8    
HELIX   65 AH2 VAL D  138  ALA D  154  1                                  17    
HELIX   66 AH3 ASP D  155  ILE D  157  5                                   3    
HELIX   67 AH4 PHE D  188  CYS D  203  1                                  16    
HELIX   68 AH5 PRO D  216  GLY D  230  1                                  15    
HELIX   69 AH6 THR D  245  SER D  252  1                                   8    
HELIX   70 AH7 SER D  264  SER D  278  1                                  15    
HELIX   71 AH8 ASP D  300  PHE D  314  1                                  15    
HELIX   72 AH9 ASN D  315  GLN D  318  5                                   4    
HELIX   73 AI1 ILE D  332  ARG D  347  1                                  16    
HELIX   74 AI2 ASP D  364  GLU D  381  1                                  18    
HELIX   75 AI3 THR D  430  ASN D  439  1                                  10    
HELIX   76 AI4 ASP D  453  MET D  464  1                                  12    
HELIX   77 AI5 PHE D  486  MET D  488  5                                   3    
HELIX   78 AI6 GLY D  496  GLU D  502  5                                   7    
SHEET    1 AA1 2 ILE A  40  ILE A  42  0                                        
SHEET    2 AA1 2 GLU A  45  GLN A  47 -1  O  GLN A  47   N  ILE A  40           
SHEET    1 AA2 2 VAL A  54  TYR A  58  0                                        
SHEET    2 AA2 2 GLN A  65  GLN A  70 -1  O  VAL A  66   N  VAL A  57           
SHEET    1 AA3 9 THR A 161  ILE A 162  0                                        
SHEET    2 AA3 9 TYR A 168  PRO A 176 -1  O  THR A 170   N  ILE A 162           
SHEET    3 AA3 9 SER A 504  LYS A 512 -1  O  VAL A 509   N  PHE A 171           
SHEET    4 AA3 9 THR B 468  ILE B 471  1  O  VAL B 469   N  THR A 508           
SHEET    5 AA3 9 VAL B 446  PHE B 450  1  N  ALA B 447   O  TRP B 470           
SHEET    6 AA3 9 ASN B 293  ILE B 295  1  N  ILE B 294   O  PHE B 450           
SHEET    7 AA3 9 ARG B 325  GLU B 329  1  O  PHE B 327   N  ILE B 295           
SHEET    8 AA3 9 VAL B 422  PHE B 428  1  O  LEU B 426   N  ILE B 326           
SHEET    9 AA3 9 THR B 402  PHE B 404  1  N  PHE B 404   O  GLN B 423           
SHEET    1 AA4 6 ILE A 236  ILE A 238  0                                        
SHEET    2 AA4 6 THR A 206  LYS A 210  1  N  ILE A 209   O  ASN A 237           
SHEET    3 AA4 6 VAL A 179  ILE A 183  1  N  GLN A 182   O  LYS A 210           
SHEET    4 AA4 6 LYS A 258  THR A 262  1  O  LYS A 258   N  GLY A 181           
SHEET    5 AA4 6 ARG A 282  GLU A 286  1  O  THR A 284   N  PHE A 261           
SHEET    6 AA4 6 GLY A 490  ASN A 491 -1  O  ASN A 491   N  LEU A 285           
SHEET    1 AA510 LYS A 384  CYS A 387  0                                        
SHEET    2 AA510 THR A 402  SER A 405 -1  O  VAL A 403   N  GLU A 386           
SHEET    3 AA510 VAL A 422  PHE A 428  1  O  GLN A 423   N  PHE A 404           
SHEET    4 AA510 ARG A 325  GLU A 329  1  N  ILE A 326   O  LEU A 426           
SHEET    5 AA510 PRO A 292  ILE A 295  1  N  ILE A 295   O  PHE A 327           
SHEET    6 AA510 VAL A 446  PHE A 450  1  O  ALA A 448   N  ILE A 294           
SHEET    7 AA510 THR A 468  ILE A 471  1  O  TRP A 470   N  ALA A 447           
SHEET    8 AA510 SER B 504  LYS B 512  1  O  THR B 508   N  VAL A 469           
SHEET    9 AA510 TYR B 168  PRO B 176 -1  N  GLU B 175   O  GLU B 505           
SHEET   10 AA510 THR B 161  ILE B 162 -1  N  ILE B 162   O  THR B 170           
SHEET    1 AA6 2 GLY A 391  LEU A 392  0                                        
SHEET    2 AA6 2 PHE A 398  ILE A 399 -1  O  PHE A 398   N  LEU A 392           
SHEET    1 AA7 2 PHE A 443  GLY A 444  0                                        
SHEET    2 AA7 2 PHE A 486  LYS A 487 -1  O  PHE A 486   N  GLY A 444           
SHEET    1 AA8 2 PHE A 483  GLY A 484  0                                        
SHEET    2 AA8 2 ARG A 493  GLU A 494 -1  O  GLU A 494   N  PHE A 483           
SHEET    1 AA9 2 ILE B  40  ILE B  42  0                                        
SHEET    2 AA9 2 GLU B  45  GLN B  47 -1  O  GLN B  47   N  ILE B  40           
SHEET    1 AB1 2 VAL B  54  TYR B  58  0                                        
SHEET    2 AB1 2 GLN B  65  GLN B  70 -1  O  VAL B  66   N  VAL B  57           
SHEET    1 AB2 5 ILE B 236  ILE B 238  0                                        
SHEET    2 AB2 5 THR B 206  LYS B 210  1  N  ILE B 209   O  ASN B 237           
SHEET    3 AB2 5 VAL B 179  ILE B 183  1  N  CYS B 180   O  THR B 206           
SHEET    4 AB2 5 LYS B 258  THR B 262  1  O  LYS B 258   N  GLY B 181           
SHEET    5 AB2 5 ARG B 282  GLU B 286  1  O  ARG B 282   N  ILE B 259           
SHEET    1 AB3 2 PHE B 483  GLY B 484  0                                        
SHEET    2 AB3 2 ARG B 493  GLU B 494 -1  O  GLU B 494   N  PHE B 483           
SHEET    1 AB4 2 ILE C  40  ILE C  42  0                                        
SHEET    2 AB4 2 GLU C  45  GLN C  47 -1  O  GLN C  47   N  ILE C  40           
SHEET    1 AB5 2 VAL C  54  TYR C  58  0                                        
SHEET    2 AB5 2 GLN C  65  GLN C  70 -1  O  VAL C  66   N  VAL C  57           
SHEET    1 AB610 THR C 161  ILE C 162  0                                        
SHEET    2 AB610 TYR C 168  PRO C 176 -1  O  THR C 170   N  ILE C 162           
SHEET    3 AB610 SER C 504  LYS C 512 -1  O  VAL C 511   N  PHE C 169           
SHEET    4 AB610 THR D 468  ILE D 471  1  O  VAL D 469   N  THR C 508           
SHEET    5 AB610 VAL D 446  PHE D 450  1  N  ALA D 447   O  TRP D 470           
SHEET    6 AB610 PRO D 292  ILE D 295  1  N  ILE D 294   O  PHE D 450           
SHEET    7 AB610 ARG D 325  GLU D 329  1  O  PHE D 327   N  ILE D 295           
SHEET    8 AB610 VAL D 422  PHE D 428  1  O  LEU D 426   N  ILE D 326           
SHEET    9 AB610 THR D 402  VAL D 407  1  N  PHE D 404   O  GLN D 423           
SHEET   10 AB610 LYS D 384  CYS D 387 -1  N  LYS D 384   O  SER D 405           
SHEET    1 AB7 6 ILE C 236  ILE C 238  0                                        
SHEET    2 AB7 6 THR C 206  LYS C 210  1  N  ILE C 209   O  ASN C 237           
SHEET    3 AB7 6 VAL C 179  ILE C 183  1  N  CYS C 180   O  THR C 206           
SHEET    4 AB7 6 LYS C 258  THR C 262  1  O  LYS C 258   N  GLY C 181           
SHEET    5 AB7 6 ARG C 282  GLU C 286  1  O  ARG C 282   N  ILE C 259           
SHEET    6 AB7 6 GLY C 490  ASN C 491 -1  O  ASN C 491   N  LEU C 285           
SHEET    1 AB810 LYS C 384  CYS C 387  0                                        
SHEET    2 AB810 THR C 402  SER C 405 -1  O  SER C 405   N  LYS C 384           
SHEET    3 AB810 VAL C 422  PHE C 428  1  O  GLN C 423   N  PHE C 404           
SHEET    4 AB810 ARG C 325  GLU C 329  1  N  ILE C 326   O  LEU C 426           
SHEET    5 AB810 PRO C 292  ILE C 295  1  N  ASN C 293   O  PHE C 327           
SHEET    6 AB810 VAL C 446  PHE C 450  1  O  ALA C 448   N  ILE C 294           
SHEET    7 AB810 THR C 468  ILE C 471  1  O  TRP C 470   N  VAL C 449           
SHEET    8 AB810 SER D 504  LYS D 512  1  O  THR D 508   N  VAL C 469           
SHEET    9 AB810 TYR D 168  PRO D 176 -1  N  GLU D 175   O  GLU D 505           
SHEET   10 AB810 THR D 161  ILE D 162 -1  N  ILE D 162   O  THR D 170           
SHEET    1 AB9 2 ILE D  40  ILE D  42  0                                        
SHEET    2 AB9 2 GLU D  45  GLN D  47 -1  O  GLN D  47   N  ILE D  40           
SHEET    1 AC1 2 VAL D  54  TYR D  58  0                                        
SHEET    2 AC1 2 GLN D  65  GLN D  70 -1  O  VAL D  66   N  VAL D  57           
SHEET    1 AC2 6 ILE D 236  ILE D 238  0                                        
SHEET    2 AC2 6 THR D 206  LYS D 210  1  N  ILE D 209   O  ASN D 237           
SHEET    3 AC2 6 VAL D 179  ILE D 183  1  N  CYS D 180   O  THR D 206           
SHEET    4 AC2 6 LYS D 258  PHE D 261  1  O  LYS D 258   N  GLY D 181           
SHEET    5 AC2 6 ARG D 282  GLU D 286  1  O  ARG D 282   N  ILE D 259           
SHEET    6 AC2 6 GLY D 490  ASN D 491 -1  O  ASN D 491   N  LEU D 285           
SHEET    1 AC3 2 GLY D 391  LEU D 392  0                                        
SHEET    2 AC3 2 PHE D 398  ILE D 399 -1  O  PHE D 398   N  LEU D 392           
CISPEP   1 TYR A  474    ASN A  475          0         2.69                     
CISPEP   2 SER B  489    GLY B  490          0         3.79                     
CISPEP   3 PHE C  483    GLY C  484          0        13.64                     
CISPEP   4 GLY C  484    GLY C  485          0        -2.54                     
CISPEP   5 THR D  262    GLY D  263          0        -0.55                     
CISPEP   6 ALA D  322    GLY D  323          0         7.54                     
SITE     1 AC1 23 ILE A 183  ILE A 184  TRP A 186  ASN A 187                    
SITE     2 AC1 23 LYS A 210  ALA A 212  GLU A 213  GLY A 243                    
SITE     3 AC1 23 GLY A 247  ALA A 248  PHE A 261  THR A 262                    
SITE     4 AC1 23 GLY A 263  SER A 264  VAL A 267  ILE A 271                    
SITE     5 AC1 23 GLU A 286  LEU A 287  GLY A 288  CYS A 320                    
SITE     6 AC1 23 GLU A 417  PHE A 419  PHE A 483                               
SITE     1 AC2 16 VAL A 138  GLY A 142  THR A 146  ASN A 187                    
SITE     2 AC2 16 PHE A 188  TRP A 195  GLN A 310  PHE A 314                    
SITE     3 AC2 16 CYS A 319  CYS A 320  THR A 321  ASN A 475                    
SITE     4 AC2 16 LEU A 477  ASN A 478  PHE A 483  MET A 495                    
SITE     1 AC3 23 ILE B 183  ILE B 184  PRO B 185  TRP B 186                    
SITE     2 AC3 23 ASN B 187  LYS B 210  GLU B 213  GLY B 243                    
SITE     3 AC3 23 GLY B 247  ALA B 248  PHE B 261  THR B 262                    
SITE     4 AC3 23 GLY B 263  SER B 264  VAL B 267  GLU B 286                    
SITE     5 AC3 23 LEU B 287  GLY B 288  CYS B 320  PHE B 419                    
SITE     6 AC3 23 LEU B 445  PHE B 483  CU4 B 602                               
SITE     1 AC4 13 GLY B 142  THR B 146  PHE B 188  LEU B 191                    
SITE     2 AC4 13 TRP B 195  GLN B 307  GLN B 310  CYS B 320                    
SITE     3 AC4 13 THR B 321  ASN B 475  LEU B 477  PHE B 483                    
SITE     4 AC4 13 NAD B 601                                                     
SITE     1 AC5 17 VAL C 138  THR C 146  ASN C 187  PHE C 188                    
SITE     2 AC5 17 MET C 192  TRP C 195  GLN C 310  PHE C 314                    
SITE     3 AC5 17 CYS C 319  CYS C 320  THR C 321  ASN C 475                    
SITE     4 AC5 17 LEU C 477  ASN C 478  ALA C 479  PHE C 483                    
SITE     5 AC5 17 NAD C 601                                                     
SITE     1 AC6 12 VAL D 138  GLY D 142  THR D 146  ASN D 187                    
SITE     2 AC6 12 PHE D 188  TRP D 195  PHE D 314  CYS D 319                    
SITE     3 AC6 12 CYS D 320  THR D 321  ASN D 475  LEU D 477                    
CRYST1   84.850  141.860  164.750  90.00  90.00  90.00 P 21 21 21   16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.011786  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.007049  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.006070        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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