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Database: PDB
Entry: 6DCN
LinkDB: 6DCN
Original site: 6DCN 
HEADER    APOPTOSIS                               07-MAY-18   6DCN              
TITLE     BCL-XL COMPLEX WITH BECLIN 1 BH3 DOMAIN T108PTHR                      
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: BCL-XL PROTEIN;                                            
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: BCL2-L-1,APOPTOSIS REGULATOR BCL-X,BCL2-L-1,APOPTOSIS       
COMPND   5 REGULATOR BCL-X;                                                     
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: BECLIN-1;                                                  
COMPND   9 CHAIN: D, C;                                                         
COMPND  10 SYNONYM: COILED-COIL MYOSIN-LIKE BCL2-INTERACTING PROTEIN,PROTEIN    
COMPND  11 GT197;                                                               
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   4 ORGANISM_COMMON: HUMAN;                                              
SOURCE   5 ORGANISM_TAXID: 9606;                                                
SOURCE   6 MOL_ID: 2;                                                           
SOURCE   7 SYNTHETIC: YES;                                                      
SOURCE   8 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   9 ORGANISM_COMMON: HUMAN;                                              
SOURCE  10 ORGANISM_TAXID: 9606                                                 
KEYWDS    APOPTOSIS, BCL-2, AUTOPHAGY, BECLIN 1                                 
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    E.F.LEE,B.J.SMITH,N.A.SMITH,S.YAO,W.D.FAIRLIE                         
REVDAT   3   17-APR-19 6DCN    1       JRNL                                     
REVDAT   2   23-JAN-19 6DCN    1       JRNL                                     
REVDAT   1   23-MAY-18 6DCN    0                                                
SPRSDE     23-MAY-18 6DCN      5VB1                                             
JRNL        AUTH   E.F.LEE,N.A.SMITH,T.P.SOARES DA COSTA,N.MEFTAHI,S.YAO,       
JRNL        AUTH 2 T.J.HARRIS,S.TRAN,A.PETTIKIRIARACHCHI,M.A.PERUGINI,          
JRNL        AUTH 3 D.W.KEIZER,M.EVANGELISTA,B.J.SMITH,W.D.FAIRLIE               
JRNL        TITL   STRUCTURAL INSIGHTS INTO BCL2 PRO-SURVIVAL PROTEIN           
JRNL        TITL 2 INTERACTIONS WITH THE KEY AUTOPHAGY REGULATOR BECN1          
JRNL        TITL 3 FOLLOWING PHOSPHORYLATION BY STK4/MST1.                      
JRNL        REF    AUTOPHAGY                     V.  15   785 2019              
JRNL        REFN                   ESSN 1554-8635                               
JRNL        PMID   30626284                                                     
JRNL        DOI    10.1080/15548627.2018.1564557                                
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.44 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.13_2998)                                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.44                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.93                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 22378                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.192                           
REMARK   3   R VALUE            (WORKING SET) : 0.189                           
REMARK   3   FREE R VALUE                     : 0.229                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 8.930                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1998                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 48.9435 -  5.8867    1.00     1603   158  0.1728 0.2176        
REMARK   3     2  5.8867 -  4.6736    1.00     1506   147  0.1721 0.2101        
REMARK   3     3  4.6736 -  4.0832    1.00     1468   144  0.1497 0.1660        
REMARK   3     4  4.0832 -  3.7100    1.00     1472   144  0.1694 0.2041        
REMARK   3     5  3.7100 -  3.4442    1.00     1455   144  0.1872 0.2141        
REMARK   3     6  3.4442 -  3.2412    1.00     1455   142  0.1961 0.2628        
REMARK   3     7  3.2412 -  3.0789    1.00     1456   143  0.2132 0.2459        
REMARK   3     8  3.0789 -  2.9449    1.00     1426   140  0.2279 0.2988        
REMARK   3     9  2.9449 -  2.8315    1.00     1448   142  0.2067 0.2623        
REMARK   3    10  2.8315 -  2.7338    1.00     1434   140  0.2183 0.2728        
REMARK   3    11  2.7338 -  2.6483    1.00     1424   141  0.2167 0.2657        
REMARK   3    12  2.6483 -  2.5726    1.00     1431   139  0.2417 0.2894        
REMARK   3    13  2.5726 -  2.5049    1.00     1428   140  0.2686 0.3179        
REMARK   3    14  2.5049 -  2.4438    0.97     1374   134  0.2941 0.3584        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.320            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 22.600           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007           2677                                  
REMARK   3   ANGLE     :  0.796           3625                                  
REMARK   3   CHIRALITY :  0.044            391                                  
REMARK   3   PLANARITY :  0.004            462                                  
REMARK   3   DIHEDRAL  : 18.299           1555                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 1                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ALL                                                    
REMARK   3    ORIGIN FOR THE GROUP (A):  14.8036   2.5550  24.0815              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2548 T22:   0.4625                                     
REMARK   3      T33:   0.3409 T12:   0.0218                                     
REMARK   3      T13:   0.0091 T23:   0.0387                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5814 L22:   2.9838                                     
REMARK   3      L33:   1.4304 L12:   0.3172                                     
REMARK   3      L13:   0.3265 L23:   0.5972                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0782 S12:  -0.0114 S13:  -0.0158                       
REMARK   3      S21:   0.0005 S22:   0.0281 S23:  -0.2726                       
REMARK   3      S31:  -0.0519 S32:   0.3274 S33:   0.0421                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6DCN COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-MAY-18.                  
REMARK 100 THE DEPOSITION ID IS D_1000234378.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 09-OCT-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON             
REMARK 200  BEAMLINE                       : MX2                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9537                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 270                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS VNOV 11, 2017                  
REMARK 200  DATA SCALING SOFTWARE          : XDS VNOV 11, 2017                  
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 22390                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.444                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 48.934                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY                : 10.80                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 11.1300                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.44                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.50                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 2P1L                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 65.09                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.52                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 2M NACL, 10% GLYCEROL, VAPOR             
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 293K                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 4 21 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -Y+1/2,X+1/2,Z                                          
REMARK 290       4555   Y+1/2,-X+1/2,Z                                          
REMARK 290       5555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       6555   X+1/2,-Y+1/2,-Z                                         
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z                                                
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       54.70950            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       54.70950            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       54.70950            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       54.70950            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       54.70950            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       54.70950            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       54.70950            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       54.70950            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, D, C                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: OCTAMERIC                  
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 19390 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 27740 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -155.0 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, D, C                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ASN A   198                                                      
REMARK 465     ALA A   199                                                      
REMARK 465     ALA A   200                                                      
REMARK 465     ALA A   201                                                      
REMARK 465     GLU A   202                                                      
REMARK 465     SER A   203                                                      
REMARK 465     ARG A   204                                                      
REMARK 465     LYS A   205                                                      
REMARK 465     GLY A   206                                                      
REMARK 465     GLN A   207                                                      
REMARK 465     GLU A   208                                                      
REMARK 465     ASN B   198                                                      
REMARK 465     ALA B   199                                                      
REMARK 465     ALA B   200                                                      
REMARK 465     ALA B   201                                                      
REMARK 465     GLU B   202                                                      
REMARK 465     SER B   203                                                      
REMARK 465     ARG B   204                                                      
REMARK 465     LYS B   205                                                      
REMARK 465     GLY B   206                                                      
REMARK 465     GLN B   207                                                      
REMARK 465     GLU B   208                                                      
REMARK 465     ASP D   105                                                      
REMARK 465     GLY D   106                                                      
REMARK 465     GLY D   128                                                      
REMARK 465     GLN D   129                                                      
REMARK 465     THR D   130                                                      
REMARK 465     ASP C   105                                                      
REMARK 465     GLY C   106                                                      
REMARK 465     GLY C   128                                                      
REMARK 465     GLN C   129                                                      
REMARK 465     THR C   130                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A  20    CG   CD   CE   NZ                                   
REMARK 470     GLN A  26    CG   CD   OE1  NE2                                  
REMARK 470     SER A 106    OG                                                  
REMARK 470     HIS A 113    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU A 129    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 184    CG   CD   OE1  OE2                                  
REMARK 470     LYS B  20    CG   CD   CE   NZ                                   
REMARK 470     SER B  23    OG                                                  
REMARK 470     GLN B  88    CG   CD   OE1  NE2                                  
REMARK 470     ASP B 107    CG   OD1  OD2                                       
REMARK 470     GLN B 111    CG   CD   OE1  NE2                                  
REMARK 470     HIS B 113    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLN B 121    CG   CD   OE1  NE2                                  
REMARK 470     GLN B 125    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 129    CG   CD   OE1  OE2                                  
REMARK 470     GLU D 110    CG   CD   OE1  OE2                                  
REMARK 470     GLU C 110    CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    PHE A 105     -149.95   -132.64                                   
REMARK 500    GLU A 184        1.15    -66.80                                   
REMARK 500    PHE B 105     -159.84   -122.71                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5VAX   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VAU   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VAY   RELATED DB: PDB                                   
DBREF  6DCN A    1    26  UNP    Q07817   B2CL1_HUMAN      1     26             
DBREF  6DCN A   83   208  UNP    Q07817   B2CL1_HUMAN     83    208             
DBREF  6DCN B    1    26  UNP    Q07817   B2CL1_HUMAN      1     26             
DBREF  6DCN B   83   208  UNP    Q07817   B2CL1_HUMAN     83    208             
DBREF  6DCN D  105   130  UNP    Q14457   BECN1_HUMAN    105    130             
DBREF  6DCN C  105   130  UNP    Q14457   BECN1_HUMAN    105    130             
SEQADV 6DCN PRO A   -3  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN LEU A   -2  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN GLY A   -1  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN SER A    0  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN PRO B   -3  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN LEU B   -2  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN GLY B   -1  UNP  Q07817              EXPRESSION TAG                 
SEQADV 6DCN SER B    0  UNP  Q07817              EXPRESSION TAG                 
SEQRES   1 A  156  PRO LEU GLY SER MET SER GLN SER ASN ARG GLU LEU VAL          
SEQRES   2 A  156  VAL ASP PHE LEU SER TYR LYS LEU SER GLN LYS GLY TYR          
SEQRES   3 A  156  SER TRP SER GLN MET ALA ALA VAL LYS GLN ALA LEU ARG          
SEQRES   4 A  156  GLU ALA GLY ASP GLU PHE GLU LEU ARG TYR ARG ARG ALA          
SEQRES   5 A  156  PHE SER ASP LEU THR SER GLN LEU HIS ILE THR PRO GLY          
SEQRES   6 A  156  THR ALA TYR GLN SER PHE GLU GLN VAL VAL ASN GLU LEU          
SEQRES   7 A  156  PHE ARG ASP GLY VAL ASN TRP GLY ARG ILE VAL ALA PHE          
SEQRES   8 A  156  PHE SER PHE GLY GLY ALA LEU CYS VAL GLU SER VAL ASP          
SEQRES   9 A  156  LYS GLU MET GLN VAL LEU VAL SER ARG ILE ALA ALA TRP          
SEQRES  10 A  156  MET ALA THR TYR LEU ASN ASP HIS LEU GLU PRO TRP ILE          
SEQRES  11 A  156  GLN GLU ASN GLY GLY TRP ASP THR PHE VAL GLU LEU TYR          
SEQRES  12 A  156  GLY ASN ASN ALA ALA ALA GLU SER ARG LYS GLY GLN GLU          
SEQRES   1 B  156  PRO LEU GLY SER MET SER GLN SER ASN ARG GLU LEU VAL          
SEQRES   2 B  156  VAL ASP PHE LEU SER TYR LYS LEU SER GLN LYS GLY TYR          
SEQRES   3 B  156  SER TRP SER GLN MET ALA ALA VAL LYS GLN ALA LEU ARG          
SEQRES   4 B  156  GLU ALA GLY ASP GLU PHE GLU LEU ARG TYR ARG ARG ALA          
SEQRES   5 B  156  PHE SER ASP LEU THR SER GLN LEU HIS ILE THR PRO GLY          
SEQRES   6 B  156  THR ALA TYR GLN SER PHE GLU GLN VAL VAL ASN GLU LEU          
SEQRES   7 B  156  PHE ARG ASP GLY VAL ASN TRP GLY ARG ILE VAL ALA PHE          
SEQRES   8 B  156  PHE SER PHE GLY GLY ALA LEU CYS VAL GLU SER VAL ASP          
SEQRES   9 B  156  LYS GLU MET GLN VAL LEU VAL SER ARG ILE ALA ALA TRP          
SEQRES  10 B  156  MET ALA THR TYR LEU ASN ASP HIS LEU GLU PRO TRP ILE          
SEQRES  11 B  156  GLN GLU ASN GLY GLY TRP ASP THR PHE VAL GLU LEU TYR          
SEQRES  12 B  156  GLY ASN ASN ALA ALA ALA GLU SER ARG LYS GLY GLN GLU          
SEQRES   1 D   26  ASP GLY GLY TPO MET GLU ASN LEU SER ARG ARG LEU LYS          
SEQRES   2 D   26  VAL THR GLY ASP LEU PHE ASP ILE MET SER GLY GLN THR          
SEQRES   1 C   26  ASP GLY GLY TPO MET GLU ASN LEU SER ARG ARG LEU LYS          
SEQRES   2 C   26  VAL THR GLY ASP LEU PHE ASP ILE MET SER GLY GLN THR          
MODRES 6DCN TPO D  108  THR  MODIFIED RESIDUE                                   
MODRES 6DCN TPO C  108  THR  MODIFIED RESIDUE                                   
HET    TPO  D 108      11                                                       
HET    TPO  C 108      11                                                       
HETNAM     TPO PHOSPHOTHREONINE                                                 
HETSYN     TPO PHOSPHONOTHREONINE                                               
FORMUL   3  TPO    2(C4 H10 N O6 P)                                             
FORMUL   5  HOH   *44(H2 O)                                                     
HELIX    1 AA1 SER A    4  LYS A   20  1                                  17    
HELIX    2 AA2 SER A   25  PHE A  105  1                                  25    
HELIX    3 AA3 THR A  118  ARG A  132  1                                  15    
HELIX    4 AA4 ASN A  136  LYS A  157  1                                  22    
HELIX    5 AA5 VAL A  161  LEU A  178  1                                  18    
HELIX    6 AA6 LEU A  178  GLU A  184  1                                   7    
HELIX    7 AA7 GLY A  186  GLY A  196  1                                  11    
HELIX    8 AA8 GLY B   -1  LYS B   20  1                                  22    
HELIX    9 AA9 SER B   25  PHE B  105  1                                  25    
HELIX   10 AB1 THR B  115  ARG B  132  1                                  18    
HELIX   11 AB2 ASN B  136  LYS B  157  1                                  22    
HELIX   12 AB3 VAL B  161  LEU B  178  1                                  18    
HELIX   13 AB4 LEU B  178  ASN B  185  1                                   8    
HELIX   14 AB5 GLY B  186  GLY B  196  1                                  11    
HELIX   15 AB6 TPO D  108  MET D  126  1                                  19    
HELIX   16 AB7 TPO C  108  MET C  126  1                                  19    
LINK         C   GLY D 107                 N   TPO D 108     1555   1555  1.33  
LINK         C   TPO D 108                 N   MET D 109     1555   1555  1.33  
LINK         C   GLY C 107                 N   TPO C 108     1555   1555  1.33  
LINK         C   TPO C 108                 N   MET C 109     1555   1555  1.33  
CRYST1  109.419  109.419   97.127  90.00  90.00  90.00 P 4 21 2     16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009139  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.009139  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010296        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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