HEADER STRUCTURAL PROTEIN 15-MAY-18 6GIY
TITLE THE BASEPLATE COMPLEX FROM THE TYPE VI SECRETION SYSTEM
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: TSSF;
COMPND 3 CHAIN: A, B;
COMPND 4 ENGINEERED: YES;
COMPND 5 MOL_ID: 2;
COMPND 6 MOLECULE: TSSG;
COMPND 7 CHAIN: C;
COMPND 8 ENGINEERED: YES;
COMPND 9 MOL_ID: 3;
COMPND 10 MOLECULE: TSSK;
COMPND 11 CHAIN: D, E, F, G, H, I;
COMPND 12 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;
SOURCE 3 ORGANISM_TAXID: 562;
SOURCE 4 GENE: EC55989_3322;
SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE 6 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE 7 MOL_ID: 2;
SOURCE 8 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;
SOURCE 9 ORGANISM_TAXID: 562;
SOURCE 10 GENE: EC55989_3321;
SOURCE 11 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE 12 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE 13 MOL_ID: 3;
SOURCE 14 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;
SOURCE 15 ORGANISM_TAXID: 562;
SOURCE 16 GENE: EC55989_3338;
SOURCE 17 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE 18 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS SECRETION, BASEPLATE, COMPLEX, STRUCTURAL PROTEIN
EXPDTA ELECTRON MICROSCOPY
AUTHOR C.RAPISARDA,R.FRONZES
REVDAT 4 18-DEC-19 6GIY 1 SCALE
REVDAT 3 05-DEC-18 6GIY 1 JRNL
REVDAT 2 24-OCT-18 6GIY 1 JRNL
REVDAT 1 17-OCT-18 6GIY 0
JRNL AUTH Y.CHERRAK,C.RAPISARDA,R.PELLARIN,G.BOUVIER,B.BARDIAUX,
JRNL AUTH 2 F.ALLAIN,C.MALOSSE,M.REY,J.CHAMOT-ROOKE,E.CASCALES,
JRNL AUTH 3 R.FRONZES,E.DURAND
JRNL TITL BIOGENESIS AND STRUCTURE OF A TYPE VI SECRETION BASEPLATE.
JRNL REF NAT MICROBIOL V. 3 1404 2018
JRNL REFN ESSN 2058-5276
JRNL PMID 30323254
JRNL DOI 10.1038/S41564-018-0260-1
REMARK 2
REMARK 2 RESOLUTION. 4.30 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 SOFTWARE PACKAGES : EPU, GCTF, UCSF CHIMERA, COOT,
REMARK 3 CRYOSPARC, RELION, RELION, PHENIX
REMARK 3 RECONSTRUCTION SCHEMA : NULL
REMARK 3
REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT
REMARK 3 PDB ENTRY : NULL
REMARK 3 REFINEMENT SPACE : REAL
REMARK 3 REFINEMENT PROTOCOL : RIGID BODY FIT
REMARK 3 REFINEMENT TARGET : NULL
REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL
REMARK 3
REMARK 3 FITTING PROCEDURE : NULL
REMARK 3
REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS
REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 4.300
REMARK 3 NUMBER OF PARTICLES : 32504
REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE
REMARK 3 CORRECTION
REMARK 3
REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL
REMARK 3
REMARK 3 OTHER DETAILS: NULL
REMARK 4
REMARK 4 6GIY COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 15-MAY-18.
REMARK 100 THE DEPOSITION ID IS D_1200010039.
REMARK 245
REMARK 245 EXPERIMENTAL DETAILS
REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE
REMARK 245 SPECIMEN TYPE : NULL
REMARK 245
REMARK 245 ELECTRON MICROSCOPE SAMPLE
REMARK 245 SAMPLE TYPE : PARTICLE
REMARK 245 PARTICLE TYPE : POINT
REMARK 245 NAME OF SAMPLE : COMPLEX OF TSSK, TSSF AND TSSG,
REMARK 245 COMPONENTS OF THE TYPE VI
REMARK 245 SECRETION SYSTEM BASEPLATE
REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : 0.20
REMARK 245 SAMPLE SUPPORT DETAILS : NULL
REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL
REMARK 245 SAMPLE BUFFER : NULL
REMARK 245 PH : 7.50
REMARK 245 SAMPLE DETAILS : NULL
REMARK 245
REMARK 245 DATA ACQUISITION
REMARK 245 DATE OF EXPERIMENT : NULL
REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL
REMARK 245 TEMPERATURE (KELVIN) : NULL
REMARK 245 MICROSCOPE MODEL : FEI TITAN KRIOS
REMARK 245 DETECTOR TYPE : GATAN K2 SUMMIT (4K X 4K)
REMARK 245 MINIMUM DEFOCUS (NM) : 700.00
REMARK 245 MAXIMUM DEFOCUS (NM) : 2000.00
REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 NOMINAL CS : 2.70
REMARK 245 IMAGING MODE : BRIGHT FIELD
REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 1.50
REMARK 245 ILLUMINATION MODE : FLOOD BEAM
REMARK 245 NOMINAL MAGNIFICATION : 130000
REMARK 245 CALIBRATED MAGNIFICATION : NULL
REMARK 245 SOURCE : FIELD EMISSION GUN
REMARK 245 ACCELERATION VOLTAGE (KV) : 300
REMARK 245 IMAGING DETAILS : NULL
REMARK 247
REMARK 247 ELECTRON MICROSCOPY
REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON
REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE
REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES
REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION
REMARK 247 OF THE STRUCTURE FACTORS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: NONAMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: NONAMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 57540 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 181310 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -295.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F, G, H, I
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 MET A 1
REMARK 465 ASP A 2
REMARK 465 ASP A 3
REMARK 465 MET B 1
REMARK 465 ASP B 2
REMARK 465 ASP B 3
REMARK 465 GLY B 587
REMARK 465 MET C 1
REMARK 465 HIS C 2
REMARK 465 PRO C 3
REMARK 465 VAL C 4
REMARK 465 GLU C 5
REMARK 465 ARG C 6
REMARK 465 LYS C 7
REMARK 465 THR C 358
REMARK 465 GLN C 359
REMARK 465 GLU C 360
REMARK 465 ASN C 361
REMARK 465 GLY C 362
REMARK 465 ASP C 363
REMARK 465 TYR C 364
REMARK 465 ARG C 365
REMARK 465 TRP C 366
REMARK 465 ALA D 312
REMARK 465 SER D 313
REMARK 465 LEU D 314
REMARK 465 PRO D 315
REMARK 465 SER D 316
REMARK 465 ARG D 317
REMARK 465 VAL D 318
REMARK 465 VAL D 319
REMARK 465 PHE D 320
REMARK 465 ILE D 321
REMARK 465 GLU D 322
REMARK 465 LEU D 323
REMARK 465 LYS D 324
REMARK 465 GLN D 325
REMARK 465 LYS D 326
REMARK 465 GLY D 327
REMARK 465 VAL D 328
REMARK 465 MET D 329
REMARK 465 TRP D 330
REMARK 465 GLU D 331
REMARK 465 GLY D 332
REMARK 465 ALA D 333
REMARK 465 LEU D 334
REMARK 465 HIS D 335
REMARK 465 ASP D 336
REMARK 465 ALA D 337
REMARK 465 ARG D 338
REMARK 465 LEU D 339
REMARK 465 ARG D 340
REMARK 465 GLU D 341
REMARK 465 GLY D 342
REMARK 465 ALA D 343
REMARK 465 ASP D 344
REMARK 465 PHE D 345
REMARK 465 TRP D 346
REMARK 465 LEU D 347
REMARK 465 SER D 348
REMARK 465 VAL D 349
REMARK 465 ARG D 350
REMARK 465 SER D 351
REMARK 465 SER D 352
REMARK 465 MET D 353
REMARK 465 PRO D 354
REMARK 465 GLY D 355
REMARK 465 HIS D 356
REMARK 465 GLU D 357
REMARK 465 LEU D 358
REMARK 465 GLN D 359
REMARK 465 THR D 360
REMARK 465 LYS D 361
REMARK 465 PHE D 362
REMARK 465 PRO D 363
REMARK 465 GLN D 364
REMARK 465 LEU D 365
REMARK 465 CYS D 366
REMARK 465 LYS D 367
REMARK 465 ALA D 368
REMARK 465 GLY D 369
REMARK 465 SER D 370
REMARK 465 PRO D 371
REMARK 465 ASP D 372
REMARK 465 ASP D 373
REMARK 465 VAL D 374
REMARK 465 SER D 375
REMARK 465 GLU D 376
REMARK 465 VAL D 377
REMARK 465 VAL D 378
REMARK 465 ASN D 379
REMARK 465 VAL D 380
REMARK 465 ALA D 381
REMARK 465 LEU D 382
REMARK 465 SER D 383
REMARK 465 GLY D 384
REMARK 465 VAL D 385
REMARK 465 ILE D 386
REMARK 465 ILE D 387
REMARK 465 ARG D 388
REMARK 465 PRO D 389
REMARK 465 VAL D 390
REMARK 465 THR D 391
REMARK 465 HIS D 392
REMARK 465 VAL D 393
REMARK 465 PRO D 394
REMARK 465 ALA D 395
REMARK 465 ALA D 396
REMARK 465 ILE D 397
REMARK 465 PRO D 398
REMARK 465 LEU D 399
REMARK 465 ARG D 400
REMARK 465 LEU D 401
REMARK 465 GLU D 402
REMARK 465 ASN D 403
REMARK 465 GLN D 404
REMARK 465 TYR D 405
REMARK 465 PHE D 406
REMARK 465 ALA D 407
REMARK 465 LEU D 408
REMARK 465 ASP D 409
REMARK 465 LEU D 410
REMARK 465 SER D 411
REMARK 465 THR D 412
REMARK 465 ASP D 413
REMARK 465 ALA D 414
REMARK 465 ALA D 415
REMARK 465 ARG D 416
REMARK 465 ALA D 417
REMARK 465 MET D 418
REMARK 465 LEU D 419
REMARK 465 ASP D 420
REMARK 465 ALA D 421
REMARK 465 GLY D 422
REMARK 465 ARG D 423
REMARK 465 CYS D 424
REMARK 465 THR D 425
REMARK 465 PHE D 426
REMARK 465 TYR D 427
REMARK 465 THR D 428
REMARK 465 PRO D 429
REMARK 465 ALA D 430
REMARK 465 SER D 431
REMARK 465 LEU D 432
REMARK 465 GLY D 433
REMARK 465 ASP D 434
REMARK 465 VAL D 435
REMARK 465 LYS D 436
REMARK 465 LEU D 437
REMARK 465 GLU D 438
REMARK 465 LEU D 439
REMARK 465 PHE D 440
REMARK 465 ALA D 441
REMARK 465 VAL D 442
REMARK 465 LEU D 443
REMARK 465 ARG D 444
REMARK 465 ALA E 312
REMARK 465 SER E 313
REMARK 465 LEU E 314
REMARK 465 PRO E 315
REMARK 465 SER E 316
REMARK 465 ARG E 317
REMARK 465 VAL E 318
REMARK 465 VAL E 319
REMARK 465 PHE E 320
REMARK 465 ILE E 321
REMARK 465 GLU E 322
REMARK 465 LEU E 323
REMARK 465 LYS E 324
REMARK 465 GLN E 325
REMARK 465 LYS E 326
REMARK 465 GLY E 327
REMARK 465 VAL E 328
REMARK 465 MET E 329
REMARK 465 TRP E 330
REMARK 465 GLU E 331
REMARK 465 GLY E 332
REMARK 465 ALA E 333
REMARK 465 LEU E 334
REMARK 465 HIS E 335
REMARK 465 ASP E 336
REMARK 465 ALA E 337
REMARK 465 ARG E 338
REMARK 465 LEU E 339
REMARK 465 ARG E 340
REMARK 465 GLU E 341
REMARK 465 GLY E 342
REMARK 465 ALA E 343
REMARK 465 ASP E 344
REMARK 465 PHE E 345
REMARK 465 TRP E 346
REMARK 465 LEU E 347
REMARK 465 SER E 348
REMARK 465 VAL E 349
REMARK 465 ARG E 350
REMARK 465 SER E 351
REMARK 465 SER E 352
REMARK 465 MET E 353
REMARK 465 PRO E 354
REMARK 465 GLY E 355
REMARK 465 HIS E 356
REMARK 465 GLU E 357
REMARK 465 LEU E 358
REMARK 465 GLN E 359
REMARK 465 THR E 360
REMARK 465 LYS E 361
REMARK 465 PHE E 362
REMARK 465 PRO E 363
REMARK 465 GLN E 364
REMARK 465 LEU E 365
REMARK 465 CYS E 366
REMARK 465 LYS E 367
REMARK 465 ALA E 368
REMARK 465 GLY E 369
REMARK 465 SER E 370
REMARK 465 PRO E 371
REMARK 465 ASP E 372
REMARK 465 ASP E 373
REMARK 465 VAL E 374
REMARK 465 SER E 375
REMARK 465 GLU E 376
REMARK 465 VAL E 377
REMARK 465 VAL E 378
REMARK 465 ASN E 379
REMARK 465 VAL E 380
REMARK 465 ALA E 381
REMARK 465 LEU E 382
REMARK 465 SER E 383
REMARK 465 GLY E 384
REMARK 465 VAL E 385
REMARK 465 ILE E 386
REMARK 465 ILE E 387
REMARK 465 ARG E 388
REMARK 465 PRO E 389
REMARK 465 VAL E 390
REMARK 465 THR E 391
REMARK 465 HIS E 392
REMARK 465 VAL E 393
REMARK 465 PRO E 394
REMARK 465 ALA E 395
REMARK 465 ALA E 396
REMARK 465 ILE E 397
REMARK 465 PRO E 398
REMARK 465 LEU E 399
REMARK 465 ARG E 400
REMARK 465 LEU E 401
REMARK 465 GLU E 402
REMARK 465 ASN E 403
REMARK 465 GLN E 404
REMARK 465 TYR E 405
REMARK 465 PHE E 406
REMARK 465 ALA E 407
REMARK 465 LEU E 408
REMARK 465 ASP E 409
REMARK 465 LEU E 410
REMARK 465 SER E 411
REMARK 465 THR E 412
REMARK 465 ASP E 413
REMARK 465 ALA E 414
REMARK 465 ALA E 415
REMARK 465 ARG E 416
REMARK 465 ALA E 417
REMARK 465 MET E 418
REMARK 465 LEU E 419
REMARK 465 ASP E 420
REMARK 465 ALA E 421
REMARK 465 GLY E 422
REMARK 465 ARG E 423
REMARK 465 CYS E 424
REMARK 465 THR E 425
REMARK 465 PHE E 426
REMARK 465 TYR E 427
REMARK 465 THR E 428
REMARK 465 PRO E 429
REMARK 465 ALA E 430
REMARK 465 SER E 431
REMARK 465 LEU E 432
REMARK 465 GLY E 433
REMARK 465 ASP E 434
REMARK 465 VAL E 435
REMARK 465 LYS E 436
REMARK 465 LEU E 437
REMARK 465 GLU E 438
REMARK 465 LEU E 439
REMARK 465 PHE E 440
REMARK 465 ALA E 441
REMARK 465 VAL E 442
REMARK 465 LEU E 443
REMARK 465 ARG E 444
REMARK 465 ALA F 312
REMARK 465 SER F 313
REMARK 465 LEU F 314
REMARK 465 PRO F 315
REMARK 465 SER F 316
REMARK 465 ARG F 317
REMARK 465 VAL F 318
REMARK 465 VAL F 319
REMARK 465 PHE F 320
REMARK 465 ILE F 321
REMARK 465 GLU F 322
REMARK 465 LEU F 323
REMARK 465 LYS F 324
REMARK 465 GLN F 325
REMARK 465 LYS F 326
REMARK 465 GLY F 327
REMARK 465 VAL F 328
REMARK 465 MET F 329
REMARK 465 TRP F 330
REMARK 465 GLU F 331
REMARK 465 GLY F 332
REMARK 465 ALA F 333
REMARK 465 LEU F 334
REMARK 465 HIS F 335
REMARK 465 ASP F 336
REMARK 465 ALA F 337
REMARK 465 ARG F 338
REMARK 465 LEU F 339
REMARK 465 ARG F 340
REMARK 465 GLU F 341
REMARK 465 GLY F 342
REMARK 465 ALA F 343
REMARK 465 ASP F 344
REMARK 465 PHE F 345
REMARK 465 TRP F 346
REMARK 465 LEU F 347
REMARK 465 SER F 348
REMARK 465 VAL F 349
REMARK 465 ARG F 350
REMARK 465 SER F 351
REMARK 465 SER F 352
REMARK 465 MET F 353
REMARK 465 PRO F 354
REMARK 465 GLY F 355
REMARK 465 HIS F 356
REMARK 465 GLU F 357
REMARK 465 LEU F 358
REMARK 465 GLN F 359
REMARK 465 THR F 360
REMARK 465 LYS F 361
REMARK 465 PHE F 362
REMARK 465 PRO F 363
REMARK 465 GLN F 364
REMARK 465 LEU F 365
REMARK 465 CYS F 366
REMARK 465 LYS F 367
REMARK 465 ALA F 368
REMARK 465 GLY F 369
REMARK 465 SER F 370
REMARK 465 PRO F 371
REMARK 465 ASP F 372
REMARK 465 ASP F 373
REMARK 465 VAL F 374
REMARK 465 SER F 375
REMARK 465 GLU F 376
REMARK 465 VAL F 377
REMARK 465 VAL F 378
REMARK 465 ASN F 379
REMARK 465 VAL F 380
REMARK 465 ALA F 381
REMARK 465 LEU F 382
REMARK 465 SER F 383
REMARK 465 GLY F 384
REMARK 465 VAL F 385
REMARK 465 ILE F 386
REMARK 465 ILE F 387
REMARK 465 ARG F 388
REMARK 465 PRO F 389
REMARK 465 VAL F 390
REMARK 465 THR F 391
REMARK 465 HIS F 392
REMARK 465 VAL F 393
REMARK 465 PRO F 394
REMARK 465 ALA F 395
REMARK 465 ALA F 396
REMARK 465 ILE F 397
REMARK 465 PRO F 398
REMARK 465 LEU F 399
REMARK 465 ARG F 400
REMARK 465 LEU F 401
REMARK 465 GLU F 402
REMARK 465 ASN F 403
REMARK 465 GLN F 404
REMARK 465 TYR F 405
REMARK 465 PHE F 406
REMARK 465 ALA F 407
REMARK 465 LEU F 408
REMARK 465 ASP F 409
REMARK 465 LEU F 410
REMARK 465 SER F 411
REMARK 465 THR F 412
REMARK 465 ASP F 413
REMARK 465 ALA F 414
REMARK 465 ALA F 415
REMARK 465 ARG F 416
REMARK 465 ALA F 417
REMARK 465 MET F 418
REMARK 465 LEU F 419
REMARK 465 ASP F 420
REMARK 465 ALA F 421
REMARK 465 GLY F 422
REMARK 465 ARG F 423
REMARK 465 CYS F 424
REMARK 465 THR F 425
REMARK 465 PHE F 426
REMARK 465 TYR F 427
REMARK 465 THR F 428
REMARK 465 PRO F 429
REMARK 465 ALA F 430
REMARK 465 SER F 431
REMARK 465 LEU F 432
REMARK 465 GLY F 433
REMARK 465 ASP F 434
REMARK 465 VAL F 435
REMARK 465 LYS F 436
REMARK 465 LEU F 437
REMARK 465 GLU F 438
REMARK 465 LEU F 439
REMARK 465 PHE F 440
REMARK 465 ALA F 441
REMARK 465 VAL F 442
REMARK 465 LEU F 443
REMARK 465 ARG F 444
REMARK 465 ALA I 312
REMARK 465 SER I 313
REMARK 465 LEU I 314
REMARK 465 PRO I 315
REMARK 465 SER I 316
REMARK 465 ARG I 317
REMARK 465 VAL I 318
REMARK 465 VAL I 319
REMARK 465 PHE I 320
REMARK 465 ILE I 321
REMARK 465 GLU I 322
REMARK 465 LEU I 323
REMARK 465 LYS I 324
REMARK 465 GLN I 325
REMARK 465 LYS I 326
REMARK 465 GLY I 327
REMARK 465 VAL I 328
REMARK 465 MET I 329
REMARK 465 TRP I 330
REMARK 465 GLU I 331
REMARK 465 GLY I 332
REMARK 465 ALA I 333
REMARK 465 LEU I 334
REMARK 465 HIS I 335
REMARK 465 ASP I 336
REMARK 465 ALA I 337
REMARK 465 ARG I 338
REMARK 465 LEU I 339
REMARK 465 ARG I 340
REMARK 465 GLU I 341
REMARK 465 GLY I 342
REMARK 465 ALA I 343
REMARK 465 ASP I 344
REMARK 465 PHE I 345
REMARK 465 TRP I 346
REMARK 465 LEU I 347
REMARK 465 SER I 348
REMARK 465 VAL I 349
REMARK 465 ARG I 350
REMARK 465 SER I 351
REMARK 465 SER I 352
REMARK 465 MET I 353
REMARK 465 PRO I 354
REMARK 465 GLY I 355
REMARK 465 HIS I 356
REMARK 465 GLU I 357
REMARK 465 LEU I 358
REMARK 465 GLN I 359
REMARK 465 THR I 360
REMARK 465 LYS I 361
REMARK 465 PHE I 362
REMARK 465 PRO I 363
REMARK 465 GLN I 364
REMARK 465 LEU I 365
REMARK 465 CYS I 366
REMARK 465 LYS I 367
REMARK 465 ALA I 368
REMARK 465 GLY I 369
REMARK 465 SER I 370
REMARK 465 PRO I 371
REMARK 465 ASP I 372
REMARK 465 ASP I 373
REMARK 465 VAL I 374
REMARK 465 SER I 375
REMARK 465 GLU I 376
REMARK 465 VAL I 377
REMARK 465 VAL I 378
REMARK 465 ASN I 379
REMARK 465 VAL I 380
REMARK 465 ALA I 381
REMARK 465 LEU I 382
REMARK 465 SER I 383
REMARK 465 GLY I 384
REMARK 465 VAL I 385
REMARK 465 ILE I 386
REMARK 465 ILE I 387
REMARK 465 ARG I 388
REMARK 465 PRO I 389
REMARK 465 VAL I 390
REMARK 465 THR I 391
REMARK 465 HIS I 392
REMARK 465 VAL I 393
REMARK 465 PRO I 394
REMARK 465 ALA I 395
REMARK 465 ALA I 396
REMARK 465 ILE I 397
REMARK 465 PRO I 398
REMARK 465 LEU I 399
REMARK 465 ARG I 400
REMARK 465 LEU I 401
REMARK 465 GLU I 402
REMARK 465 ASN I 403
REMARK 465 GLN I 404
REMARK 465 TYR I 405
REMARK 465 PHE I 406
REMARK 465 ALA I 407
REMARK 465 LEU I 408
REMARK 465 ASP I 409
REMARK 465 LEU I 410
REMARK 465 SER I 411
REMARK 465 THR I 412
REMARK 465 ASP I 413
REMARK 465 ALA I 414
REMARK 465 ALA I 415
REMARK 465 ARG I 416
REMARK 465 ALA I 417
REMARK 465 MET I 418
REMARK 465 LEU I 419
REMARK 465 ASP I 420
REMARK 465 ALA I 421
REMARK 465 GLY I 422
REMARK 465 ARG I 423
REMARK 465 CYS I 424
REMARK 465 THR I 425
REMARK 465 PHE I 426
REMARK 465 TYR I 427
REMARK 465 THR I 428
REMARK 465 PRO I 429
REMARK 465 ALA I 430
REMARK 465 SER I 431
REMARK 465 LEU I 432
REMARK 465 GLY I 433
REMARK 465 ASP I 434
REMARK 465 VAL I 435
REMARK 465 LYS I 436
REMARK 465 LEU I 437
REMARK 465 GLU I 438
REMARK 465 LEU I 439
REMARK 465 PHE I 440
REMARK 465 ALA I 441
REMARK 465 VAL I 442
REMARK 465 LEU I 443
REMARK 465 ARG I 444
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470 M RES CSSEQI ATOMS
REMARK 470 SER C 334 OG
REMARK 470 ARG C 337 CG CD NE CZ NH1 NH2
REMARK 470 VAL C 338 CG1 CG2
REMARK 470 MET C 339 CG SD CE
REMARK 470 THR C 340 OG1 CG2
REMARK 470 ILE C 341 CG1 CG2 CD1
REMARK 470 SER C 342 OG
REMARK 470 LEU C 343 CG CD1 CD2
REMARK 470 ASN D 222 CG OD1 ND2
REMARK 470 ASN D 223 CG OD1 ND2
REMARK 470 ARG D 225 CG CD NE CZ NH1 NH2
REMARK 470 ARG E 225 CG CD NE CZ NH1 NH2
REMARK 470 ASN F 159 CG OD1 ND2
REMARK 470 LEU H 334 CG CD1 CD2
REMARK 470 HIS H 335 CG ND1 CD2 CE1 NE2
REMARK 470 LEU H 347 CG CD1 CD2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500 M RES CSSEQI ATM1 ATM2 ATM3
REMARK 500 TYR C 346 C - N - CA ANGL. DEV. = 15.1 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 PRO A 109 52.14 -91.55
REMARK 500 GLU A 113 -168.21 -125.91
REMARK 500 SER A 154 111.33 -160.49
REMARK 500 GLU A 164 37.67 -93.10
REMARK 500 TYR A 202 30.82 -89.06
REMARK 500 ASN A 208 28.96 -143.23
REMARK 500 ARG A 213 14.67 56.25
REMARK 500 GLU A 226 32.21 -91.39
REMARK 500 TYR A 240 23.78 -141.51
REMARK 500 HIS A 257 58.43 -97.96
REMARK 500 GLU A 276 -11.02 -140.23
REMARK 500 ASP A 291 54.14 -96.48
REMARK 500 TYR A 321 -39.57 -132.75
REMARK 500 ARG A 346 -60.02 -100.10
REMARK 500 HIS A 360 67.71 61.21
REMARK 500 TYR A 374 108.22 -162.39
REMARK 500 ASP A 387 139.33 -170.63
REMARK 500 THR A 388 119.70 -164.04
REMARK 500 GLU A 398 77.31 -100.48
REMARK 500 VAL A 435 -76.01 -97.60
REMARK 500 LEU A 436 63.92 21.68
REMARK 500 LEU A 476 -159.48 -79.59
REMARK 500 LEU A 501 53.39 -90.51
REMARK 500 ASP A 531 125.52 -32.65
REMARK 500 GLN A 569 153.54 -49.57
REMARK 500 PRO A 570 78.11 -66.03
REMARK 500 PRO B 28 5.52 -64.90
REMARK 500 LEU B 68 73.96 52.60
REMARK 500 LEU B 84 5.63 -68.20
REMARK 500 PRO B 88 -168.88 -76.93
REMARK 500 MET B 102 112.82 -161.83
REMARK 500 ALA B 161 -60.37 -93.30
REMARK 500 ALA B 181 -161.57 -79.40
REMARK 500 ALA B 182 22.73 -78.02
REMARK 500 SER B 187 49.01 -85.30
REMARK 500 SER B 188 -2.47 -141.30
REMARK 500 SER B 238 108.55 -55.49
REMARK 500 PHE B 247 66.78 61.25
REMARK 500 THR B 248 -17.39 -140.11
REMARK 500 PHE B 249 70.11 45.22
REMARK 500 MET B 254 77.01 57.84
REMARK 500 PHE B 255 117.31 -34.61
REMARK 500 ASN B 259 -168.02 -75.34
REMARK 500 LEU B 275 45.09 -141.00
REMARK 500 LEU B 317 -165.17 -125.97
REMARK 500 TYR B 321 30.48 -140.39
REMARK 500 VAL B 353 72.73 57.21
REMARK 500 TYR B 373 -169.31 -118.87
REMARK 500 GLN B 418 -167.21 -161.53
REMARK 500 HIS B 461 4.55 -69.47
REMARK 500
REMARK 500 THIS ENTRY HAS 163 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION. CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS. TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500 MODEL OMEGA
REMARK 500 LEU A 178 TYR A 179 -149.25
REMARK 500 GLU A 276 VAL A 277 -148.02
REMARK 500 HIS A 360 ARG A 361 147.57
REMARK 500 LEU A 390 ILE A 391 -149.74
REMARK 500 VAL A 435 LEU A 436 128.27
REMARK 500 TYR A 453 PRO A 454 144.82
REMARK 500 ILE A 504 LEU A 505 -145.41
REMARK 500 LEU A 530 ASP A 531 -140.87
REMARK 500 ASN A 562 GLN A 563 -145.89
REMARK 500 TRP B 80 PRO B 81 147.52
REMARK 500 ARG B 117 PRO B 118 -149.84
REMARK 500 PHE B 247 THR B 248 141.58
REMARK 500 LEU B 275 GLU B 276 -149.96
REMARK 500 PHE B 515 GLU B 516 149.05
REMARK 500 ARG B 576 TRP B 577 -147.28
REMARK 500 LYS B 578 GLU B 579 147.67
REMARK 500 GLU B 579 ASN B 580 140.79
REMARK 500 GLN C 110 GLN C 111 -149.32
REMARK 500 TYR C 142 PRO C 143 -130.38
REMARK 500 ILE C 166 PRO C 167 145.78
REMARK 500 ARG C 219 ILE C 220 -117.54
REMARK 500 ILE C 220 PRO C 221 -115.79
REMARK 500 LYS C 237 SER C 238 -137.66
REMARK 500 VAL C 249 ASN C 250 147.71
REMARK 500 GLU C 299 ARG C 300 -133.17
REMARK 500 ASP C 305 ALA C 306 -138.50
REMARK 500 LEU C 308 SER C 309 -132.28
REMARK 500 CYS C 310 ARG C 311 123.12
REMARK 500 GLN C 328 ALA C 329 -149.56
REMARK 500 TYR C 346 GLN C 347 135.98
REMARK 500 GLN C 347 ARG C 348 -118.04
REMARK 500 THR E 190 LEU E 191 -141.66
REMARK 500 HIS F 40 PRO F 41 144.61
REMARK 500 TRP F 162 LEU F 163 -125.68
REMARK 500 HIS G 40 PRO G 41 130.73
REMARK 500 GLN G 133 GLU G 134 -132.07
REMARK 500 ALA G 155 HIS G 156 -142.42
REMARK 500 ALA G 160 ALA G 161 146.98
REMARK 500 MET G 256 PRO G 257 -138.39
REMARK 500 LEU H 56 SER H 57 -136.77
REMARK 500 ALA I 12 PHE I 13 -146.58
REMARK 500 PRO I 41 TRP I 42 -146.88
REMARK 500 LEU I 56 SER I 57 -133.44
REMARK 500 GLU I 139 GLN I 140 -147.99
REMARK 500 ALA I 160 ALA I 161 148.98
REMARK 500 PRO I 186 PRO I 187 -144.11
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: EMD-0008 RELATED DB: EMDB
REMARK 900 THE BASEPLATE COMPLEX FROM THE TYPE VI SECRETION SYSTEM
DBREF 6GIY A 1 587 UNP B7LFT7 B7LFT7_ECO55 1 587
DBREF 6GIY B 1 587 UNP B7LFT7 B7LFT7_ECO55 1 587
DBREF 6GIY C 1 366 UNP B7LFT6 B7LFT6_ECO55 1 366
DBREF 6GIY D 1 444 UNP B7LG64 B7LG64_ECO55 1 444
DBREF 6GIY E 1 444 UNP B7LG64 B7LG64_ECO55 1 444
DBREF 6GIY F 1 444 UNP B7LG64 B7LG64_ECO55 1 444
DBREF 6GIY G 1 444 UNP B7LG64 B7LG64_ECO55 1 444
DBREF 6GIY H 1 444 UNP B7LG64 B7LG64_ECO55 1 444
DBREF 6GIY I 1 444 UNP B7LG64 B7LG64_ECO55 1 444
SEQADV 6GIY SER D 194 UNP B7LG64 GLY 194 CONFLICT
SEQADV 6GIY LEU D 202 UNP B7LG64 ALA 202 CONFLICT
SEQADV 6GIY SER E 194 UNP B7LG64 GLY 194 CONFLICT
SEQADV 6GIY LEU E 202 UNP B7LG64 ALA 202 CONFLICT
SEQADV 6GIY SER F 194 UNP B7LG64 GLY 194 CONFLICT
SEQADV 6GIY LEU F 202 UNP B7LG64 ALA 202 CONFLICT
SEQADV 6GIY SER G 194 UNP B7LG64 GLY 194 CONFLICT
SEQADV 6GIY LEU G 202 UNP B7LG64 ALA 202 CONFLICT
SEQADV 6GIY SER H 194 UNP B7LG64 GLY 194 CONFLICT
SEQADV 6GIY LEU H 202 UNP B7LG64 ALA 202 CONFLICT
SEQADV 6GIY SER I 194 UNP B7LG64 GLY 194 CONFLICT
SEQADV 6GIY LEU I 202 UNP B7LG64 ALA 202 CONFLICT
SEQRES 1 A 587 MET ASP ASP LEU THR LEU ARG TYR PHE ASP ALA GLU MET
SEQRES 2 A 587 ARG TYR LEU ARG GLU ALA GLY LYS ALA PHE ALA GLN ALA
SEQRES 3 A 587 HIS PRO ASP ARG ALA ALA MET LEU ASP LEU ASP LYS ALA
SEQRES 4 A 587 GLY THR PRO ASP PRO CYS VAL GLU ARG LEU PHE GLU GLY
SEQRES 5 A 587 PHE ALA PHE SER MET GLY ARG LEU ARG GLN LYS ILE ASP
SEQRES 6 A 587 ASP ASP LEU PRO GLU LEU THR GLU SER LEU VAL SER MET
SEQRES 7 A 587 LEU TRP PRO HIS TYR LEU ARG THR ILE PRO SER LEU SER
SEQRES 8 A 587 VAL VAL ALA LEU THR PRO ARG LEU SER VAL MET LYS MET
SEQRES 9 A 587 ALA GLU THR VAL PRO ALA GLY LEU GLU VAL THR SER ARG
SEQRES 10 A 587 PRO VAL GLY PRO GLY ASN THR VAL CYS ARG TYR ARG THR
SEQRES 11 A 587 THR ARG ALA ILE PRO LEU ASN PRO LEU ALA VAL GLU LYS
SEQRES 12 A 587 VAL VAL MET THR THR GLU PRO ASP GLY ARG SER VAL LEU
SEQRES 13 A 587 LYS ILE GLY PHE ALA CYS SER GLU LEU ALA ASP TRP SER
SEQRES 14 A 587 GLN VAL ASP LEU HIS ARG LEU SER LEU TYR LEU ALA ALA
SEQRES 15 A 587 GLU ALA PRO VAL SER SER THR LEU HIS LEU MET MET THR
SEQRES 16 A 587 LYS ARG LEU ALA ALA LEU TYR LEU ARG LEU PRO GLY ASN
SEQRES 17 A 587 ASP GLU ARG ILE ARG ILE ASP GLY TRP PHE SER PRO GLY
SEQRES 18 A 587 GLY PHE ALA GLU GLU ASP ARG LEU TRP PRO LYS GLY ASP
SEQRES 19 A 587 SER ALA PHE SER GLY TYR GLN LEU LEU LEU GLU TYR PHE
SEQRES 20 A 587 THR PHE ARG GLU LYS PHE MET PHE VAL HIS LEU ASN GLY
SEQRES 21 A 587 LEU GLU ASN VAL SER LEU PRO ALA GLY ILE SER GLY PHE
SEQRES 22 A 587 ASP LEU GLU VAL VAL LEU SER GLN PRO TRP PRO ALA ASP
SEQRES 23 A 587 LEU PRO VAL THR ASP ASP ALA LEU CYS LEU HIS CYS VAL
SEQRES 24 A 587 PRO VAL ILE ASN LEU PHE THR LEU GLU ALA ASP PRO LEU
SEQRES 25 A 587 ILE ILE ASN GLY LEU GLU SER GLU TYR LEU LEU ARG PRO
SEQRES 26 A 587 LYS ARG LEU GLN ASP GLY TYR THR GLU ILE TYR SER VAL
SEQRES 27 A 587 ASP ALA VAL THR GLY SER GLY ARG THR GLY SER ALA GLU
SEQRES 28 A 587 TYR VAL PRO PHE THR SER PHE ARG HIS ARG GLY GLY MET
SEQRES 29 A 587 LEU ARG HIS ASP ALA PRO GLU ARG TYR TYR HIS THR ARG
SEQRES 30 A 587 VAL LYS ARG GLY VAL THR GLY MET TYR ASP THR TRP LEU
SEQRES 31 A 587 ILE LEU GLY GLY GLN ARG TRP GLU ALA ASP ARG MET PRO
SEQRES 32 A 587 GLU ARG GLU THR LEU SER LEU ARG ILE THR GLY THR ASN
SEQRES 33 A 587 GLY GLN LEU PRO ARG ARG ALA LEU GLN SER THR LEU LEU
SEQRES 34 A 587 ASP ARG CYS GLU GLN VAL LEU GLN ALA PRO VAL SER VAL
SEQRES 35 A 587 ARG ASN LEU CYS LYS PRO THR LEU PRO VAL TYR PRO PRO
SEQRES 36 A 587 THR GLU ASP ARG PHE HIS TRP ARG VAL MET SER HIS LEU
SEQRES 37 A 587 GLY THR GLY PHE LEU ASN MET LEU SER SER ALA GLU VAL
SEQRES 38 A 587 LEU ARG GLY THR LEU ALA LEU TYR ASN TRP ARG ASP ASP
SEQRES 39 A 587 GLU LEU ASN HIS ARG ARG LEU ASP ALA ILE LEU ALA VAL
SEQRES 40 A 587 GLN HIS HIS ARG ILE GLN ARG PHE GLU LYS GLY PHE LEU
SEQRES 41 A 587 LEU ARG GLY LEU ASP VAL GLU VAL THR LEU ASP GLY ASN
SEQRES 42 A 587 GLY PHE ALA GLY GLU GLY ASP ILE HIS LEU PHE GLY GLU
SEQRES 43 A 587 MET LEU ASN ARG PHE LEU ALA LEU TYR ALA ASP MET ASN
SEQRES 44 A 587 GLN PHE ASN GLN LEU THR LEU ILE VAL GLN PRO GLU GLY
SEQRES 45 A 587 LYS CYS ILE ARG TRP LYS GLU ASN HIS ASN PRO ARG LEU
SEQRES 46 A 587 PRO GLY
SEQRES 1 B 587 MET ASP ASP LEU THR LEU ARG TYR PHE ASP ALA GLU MET
SEQRES 2 B 587 ARG TYR LEU ARG GLU ALA GLY LYS ALA PHE ALA GLN ALA
SEQRES 3 B 587 HIS PRO ASP ARG ALA ALA MET LEU ASP LEU ASP LYS ALA
SEQRES 4 B 587 GLY THR PRO ASP PRO CYS VAL GLU ARG LEU PHE GLU GLY
SEQRES 5 B 587 PHE ALA PHE SER MET GLY ARG LEU ARG GLN LYS ILE ASP
SEQRES 6 B 587 ASP ASP LEU PRO GLU LEU THR GLU SER LEU VAL SER MET
SEQRES 7 B 587 LEU TRP PRO HIS TYR LEU ARG THR ILE PRO SER LEU SER
SEQRES 8 B 587 VAL VAL ALA LEU THR PRO ARG LEU SER VAL MET LYS MET
SEQRES 9 B 587 ALA GLU THR VAL PRO ALA GLY LEU GLU VAL THR SER ARG
SEQRES 10 B 587 PRO VAL GLY PRO GLY ASN THR VAL CYS ARG TYR ARG THR
SEQRES 11 B 587 THR ARG ALA ILE PRO LEU ASN PRO LEU ALA VAL GLU LYS
SEQRES 12 B 587 VAL VAL MET THR THR GLU PRO ASP GLY ARG SER VAL LEU
SEQRES 13 B 587 LYS ILE GLY PHE ALA CYS SER GLU LEU ALA ASP TRP SER
SEQRES 14 B 587 GLN VAL ASP LEU HIS ARG LEU SER LEU TYR LEU ALA ALA
SEQRES 15 B 587 GLU ALA PRO VAL SER SER THR LEU HIS LEU MET MET THR
SEQRES 16 B 587 LYS ARG LEU ALA ALA LEU TYR LEU ARG LEU PRO GLY ASN
SEQRES 17 B 587 ASP GLU ARG ILE ARG ILE ASP GLY TRP PHE SER PRO GLY
SEQRES 18 B 587 GLY PHE ALA GLU GLU ASP ARG LEU TRP PRO LYS GLY ASP
SEQRES 19 B 587 SER ALA PHE SER GLY TYR GLN LEU LEU LEU GLU TYR PHE
SEQRES 20 B 587 THR PHE ARG GLU LYS PHE MET PHE VAL HIS LEU ASN GLY
SEQRES 21 B 587 LEU GLU ASN VAL SER LEU PRO ALA GLY ILE SER GLY PHE
SEQRES 22 B 587 ASP LEU GLU VAL VAL LEU SER GLN PRO TRP PRO ALA ASP
SEQRES 23 B 587 LEU PRO VAL THR ASP ASP ALA LEU CYS LEU HIS CYS VAL
SEQRES 24 B 587 PRO VAL ILE ASN LEU PHE THR LEU GLU ALA ASP PRO LEU
SEQRES 25 B 587 ILE ILE ASN GLY LEU GLU SER GLU TYR LEU LEU ARG PRO
SEQRES 26 B 587 LYS ARG LEU GLN ASP GLY TYR THR GLU ILE TYR SER VAL
SEQRES 27 B 587 ASP ALA VAL THR GLY SER GLY ARG THR GLY SER ALA GLU
SEQRES 28 B 587 TYR VAL PRO PHE THR SER PHE ARG HIS ARG GLY GLY MET
SEQRES 29 B 587 LEU ARG HIS ASP ALA PRO GLU ARG TYR TYR HIS THR ARG
SEQRES 30 B 587 VAL LYS ARG GLY VAL THR GLY MET TYR ASP THR TRP LEU
SEQRES 31 B 587 ILE LEU GLY GLY GLN ARG TRP GLU ALA ASP ARG MET PRO
SEQRES 32 B 587 GLU ARG GLU THR LEU SER LEU ARG ILE THR GLY THR ASN
SEQRES 33 B 587 GLY GLN LEU PRO ARG ARG ALA LEU GLN SER THR LEU LEU
SEQRES 34 B 587 ASP ARG CYS GLU GLN VAL LEU GLN ALA PRO VAL SER VAL
SEQRES 35 B 587 ARG ASN LEU CYS LYS PRO THR LEU PRO VAL TYR PRO PRO
SEQRES 36 B 587 THR GLU ASP ARG PHE HIS TRP ARG VAL MET SER HIS LEU
SEQRES 37 B 587 GLY THR GLY PHE LEU ASN MET LEU SER SER ALA GLU VAL
SEQRES 38 B 587 LEU ARG GLY THR LEU ALA LEU TYR ASN TRP ARG ASP ASP
SEQRES 39 B 587 GLU LEU ASN HIS ARG ARG LEU ASP ALA ILE LEU ALA VAL
SEQRES 40 B 587 GLN HIS HIS ARG ILE GLN ARG PHE GLU LYS GLY PHE LEU
SEQRES 41 B 587 LEU ARG GLY LEU ASP VAL GLU VAL THR LEU ASP GLY ASN
SEQRES 42 B 587 GLY PHE ALA GLY GLU GLY ASP ILE HIS LEU PHE GLY GLU
SEQRES 43 B 587 MET LEU ASN ARG PHE LEU ALA LEU TYR ALA ASP MET ASN
SEQRES 44 B 587 GLN PHE ASN GLN LEU THR LEU ILE VAL GLN PRO GLU GLY
SEQRES 45 B 587 LYS CYS ILE ARG TRP LYS GLU ASN HIS ASN PRO ARG LEU
SEQRES 46 B 587 PRO GLY
SEQRES 1 C 366 MET HIS PRO VAL GLU ARG LYS SER GLN SER ALA PRO ALA
SEQRES 2 C 366 ARG LEU ILE THR ARG TYR ARG LYS GLN LEU PRO TYR ILE
SEQRES 3 C 366 ASN PHE TYR ARG PHE CYS GLN LEU LEU GLU GLN SER GLN
SEQRES 4 C 366 PRO ASP GLN PRO PRO ILE GLY SER GLY TRP GLN ALA ARG
SEQRES 5 C 366 GLN GLU ALA VAL ARG PHE CYS PRO TYR PRO GLY MET GLY
SEQRES 6 C 366 PHE PRO ALA SER GLU ILE LYS ASP ALA VAL ILE PRO GLU
SEQRES 7 C 366 GLU SER HIS LEU PRO PRO ILE VAL HIS VAL THR PHE MET
SEQRES 8 C 366 GLY LEU TYR GLY VAL THR SER PRO LEU PRO ALA HIS TYR
SEQRES 9 C 366 ILE SER ASP ILE ALA GLN GLN ARG GLU GLY HIS GLU ALA
SEQRES 10 C 366 ALA ALA ASP PHE LEU ASP ILE PHE SER HIS ARG LEU ILE
SEQRES 11 C 366 THR GLN TYR TYR ARG ILE TRP ARG LYS TYR SER TYR PRO
SEQRES 12 C 366 ALA THR PHE GLU ALA GLY GLY GLN ASP LYS THR SER GLN
SEQRES 13 C 366 TYR LEU LEU GLY LEU ALA ARG LEU GLY ILE PRO GLY CYS
SEQRES 14 C 366 ALA GLN ASN ILE ALA THR PRO VAL SER ARG PHE LEU ALA
SEQRES 15 C 366 LEU LEU PRO LEU MET LEU LEU PRO GLY ARG THR ALA GLU
SEQRES 16 C 366 GLY LEU THR SER LEU VAL THR LEU LEU ALA PRO GLY THR
SEQRES 17 C 366 GLN ALA ARG VAL TRP HIS HIS ASP ARG ARG ARG ILE PRO
SEQRES 18 C 366 LEU LYS THR PRO LEU THR MET ARG VAL HIS HIS PRO VAL
SEQRES 19 C 366 SER LEU LYS SER ARG PRO VAL MET GLY ASP HIS ALA THR
SEQRES 20 C 366 ASP VAL ASN GLY GLN VAL LEU LEU GLN LEU SER THR GLN
SEQRES 21 C 366 THR GLY SER GLU VAL GLN GLY TRP LEU PRO GLY GLY HIS
SEQRES 22 C 366 LEU TYR SER ASP LEU LEU ALA LEU LEU HIS VAL TYR LEU
SEQRES 23 C 366 GLY SER ARG LEU ASP VAL ARG LEU GLN LEU CYS VAL GLU
SEQRES 24 C 366 ARG SER LEU LEU PRO ASP ALA ARG LEU SER CYS ARG PRO
SEQRES 25 C 366 ALA ALA GLY SER PRO GLN LEU GLY ARG THR ALA VAL MET
SEQRES 26 C 366 ARG THR GLN ALA LYS ILE ALA THR SER ALA ALA ARG VAL
SEQRES 27 C 366 MET THR ILE SER LEU GLY ARG TYR GLN ARG VAL GLN GLU
SEQRES 28 C 366 HIS TYR GLN ARG LYS GLU THR GLN GLU ASN GLY ASP TYR
SEQRES 29 C 366 ARG TRP
SEQRES 1 D 444 MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE
SEQRES 2 D 444 LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP
SEQRES 3 D 444 VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA
SEQRES 4 D 444 HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER
SEQRES 5 D 444 LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE
SEQRES 6 D 444 VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG
SEQRES 7 D 444 ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL
SEQRES 8 D 444 SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO
SEQRES 9 D 444 LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER
SEQRES 10 D 444 GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL
SEQRES 11 D 444 ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL
SEQRES 12 D 444 ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS
SEQRES 13 D 444 GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG
SEQRES 14 D 444 LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO
SEQRES 15 D 444 ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO
SEQRES 16 D 444 SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU
SEQRES 17 D 444 GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU
SEQRES 18 D 444 ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL
SEQRES 19 D 444 SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU
SEQRES 20 D 444 PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS
SEQRES 21 D 444 PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY
SEQRES 22 D 444 SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA
SEQRES 23 D 444 VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE
SEQRES 24 D 444 PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA
SEQRES 25 D 444 SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN
SEQRES 26 D 444 LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG
SEQRES 27 D 444 LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER
SEQRES 28 D 444 SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN
SEQRES 29 D 444 LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL
SEQRES 30 D 444 VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL
SEQRES 31 D 444 THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN
SEQRES 32 D 444 GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG
SEQRES 33 D 444 ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO
SEQRES 34 D 444 ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL
SEQRES 35 D 444 LEU ARG
SEQRES 1 E 444 MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE
SEQRES 2 E 444 LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP
SEQRES 3 E 444 VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA
SEQRES 4 E 444 HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER
SEQRES 5 E 444 LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE
SEQRES 6 E 444 VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG
SEQRES 7 E 444 ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL
SEQRES 8 E 444 SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO
SEQRES 9 E 444 LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER
SEQRES 10 E 444 GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL
SEQRES 11 E 444 ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL
SEQRES 12 E 444 ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS
SEQRES 13 E 444 GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG
SEQRES 14 E 444 LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO
SEQRES 15 E 444 ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO
SEQRES 16 E 444 SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU
SEQRES 17 E 444 GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU
SEQRES 18 E 444 ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL
SEQRES 19 E 444 SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU
SEQRES 20 E 444 PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS
SEQRES 21 E 444 PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY
SEQRES 22 E 444 SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA
SEQRES 23 E 444 VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE
SEQRES 24 E 444 PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA
SEQRES 25 E 444 SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN
SEQRES 26 E 444 LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG
SEQRES 27 E 444 LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER
SEQRES 28 E 444 SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN
SEQRES 29 E 444 LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL
SEQRES 30 E 444 VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL
SEQRES 31 E 444 THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN
SEQRES 32 E 444 GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG
SEQRES 33 E 444 ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO
SEQRES 34 E 444 ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL
SEQRES 35 E 444 LEU ARG
SEQRES 1 F 444 MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE
SEQRES 2 F 444 LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP
SEQRES 3 F 444 VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA
SEQRES 4 F 444 HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER
SEQRES 5 F 444 LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE
SEQRES 6 F 444 VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG
SEQRES 7 F 444 ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL
SEQRES 8 F 444 SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO
SEQRES 9 F 444 LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER
SEQRES 10 F 444 GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL
SEQRES 11 F 444 ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL
SEQRES 12 F 444 ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS
SEQRES 13 F 444 GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG
SEQRES 14 F 444 LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO
SEQRES 15 F 444 ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO
SEQRES 16 F 444 SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU
SEQRES 17 F 444 GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU
SEQRES 18 F 444 ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL
SEQRES 19 F 444 SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU
SEQRES 20 F 444 PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS
SEQRES 21 F 444 PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY
SEQRES 22 F 444 SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA
SEQRES 23 F 444 VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE
SEQRES 24 F 444 PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA
SEQRES 25 F 444 SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN
SEQRES 26 F 444 LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG
SEQRES 27 F 444 LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER
SEQRES 28 F 444 SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN
SEQRES 29 F 444 LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL
SEQRES 30 F 444 VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL
SEQRES 31 F 444 THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN
SEQRES 32 F 444 GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG
SEQRES 33 F 444 ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO
SEQRES 34 F 444 ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL
SEQRES 35 F 444 LEU ARG
SEQRES 1 G 444 MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE
SEQRES 2 G 444 LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP
SEQRES 3 G 444 VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA
SEQRES 4 G 444 HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER
SEQRES 5 G 444 LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE
SEQRES 6 G 444 VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG
SEQRES 7 G 444 ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL
SEQRES 8 G 444 SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO
SEQRES 9 G 444 LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER
SEQRES 10 G 444 GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL
SEQRES 11 G 444 ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL
SEQRES 12 G 444 ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS
SEQRES 13 G 444 GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG
SEQRES 14 G 444 LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO
SEQRES 15 G 444 ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO
SEQRES 16 G 444 SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU
SEQRES 17 G 444 GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU
SEQRES 18 G 444 ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL
SEQRES 19 G 444 SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU
SEQRES 20 G 444 PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS
SEQRES 21 G 444 PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY
SEQRES 22 G 444 SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA
SEQRES 23 G 444 VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE
SEQRES 24 G 444 PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA
SEQRES 25 G 444 SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN
SEQRES 26 G 444 LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG
SEQRES 27 G 444 LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER
SEQRES 28 G 444 SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN
SEQRES 29 G 444 LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL
SEQRES 30 G 444 VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL
SEQRES 31 G 444 THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN
SEQRES 32 G 444 GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG
SEQRES 33 G 444 ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO
SEQRES 34 G 444 ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL
SEQRES 35 G 444 LEU ARG
SEQRES 1 H 444 MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE
SEQRES 2 H 444 LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP
SEQRES 3 H 444 VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA
SEQRES 4 H 444 HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER
SEQRES 5 H 444 LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE
SEQRES 6 H 444 VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG
SEQRES 7 H 444 ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL
SEQRES 8 H 444 SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO
SEQRES 9 H 444 LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER
SEQRES 10 H 444 GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL
SEQRES 11 H 444 ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL
SEQRES 12 H 444 ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS
SEQRES 13 H 444 GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG
SEQRES 14 H 444 LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO
SEQRES 15 H 444 ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO
SEQRES 16 H 444 SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU
SEQRES 17 H 444 GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU
SEQRES 18 H 444 ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL
SEQRES 19 H 444 SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU
SEQRES 20 H 444 PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS
SEQRES 21 H 444 PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY
SEQRES 22 H 444 SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA
SEQRES 23 H 444 VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE
SEQRES 24 H 444 PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA
SEQRES 25 H 444 SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN
SEQRES 26 H 444 LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG
SEQRES 27 H 444 LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER
SEQRES 28 H 444 SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN
SEQRES 29 H 444 LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL
SEQRES 30 H 444 VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL
SEQRES 31 H 444 THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN
SEQRES 32 H 444 GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG
SEQRES 33 H 444 ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO
SEQRES 34 H 444 ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL
SEQRES 35 H 444 LEU ARG
SEQRES 1 I 444 MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE
SEQRES 2 I 444 LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP
SEQRES 3 I 444 VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA
SEQRES 4 I 444 HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER
SEQRES 5 I 444 LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE
SEQRES 6 I 444 VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG
SEQRES 7 I 444 ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL
SEQRES 8 I 444 SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO
SEQRES 9 I 444 LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER
SEQRES 10 I 444 GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL
SEQRES 11 I 444 ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL
SEQRES 12 I 444 ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS
SEQRES 13 I 444 GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG
SEQRES 14 I 444 LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO
SEQRES 15 I 444 ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO
SEQRES 16 I 444 SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU
SEQRES 17 I 444 GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU
SEQRES 18 I 444 ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL
SEQRES 19 I 444 SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU
SEQRES 20 I 444 PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS
SEQRES 21 I 444 PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY
SEQRES 22 I 444 SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA
SEQRES 23 I 444 VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE
SEQRES 24 I 444 PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA
SEQRES 25 I 444 SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN
SEQRES 26 I 444 LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG
SEQRES 27 I 444 LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER
SEQRES 28 I 444 SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN
SEQRES 29 I 444 LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL
SEQRES 30 I 444 VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL
SEQRES 31 I 444 THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN
SEQRES 32 I 444 GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG
SEQRES 33 I 444 ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO
SEQRES 34 I 444 ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL
SEQRES 35 I 444 LEU ARG
HELIX 1 AA1 THR A 5 LYS A 21 1 17
HELIX 2 AA2 VAL A 46 GLU A 51 1 6
HELIX 3 AA3 PHE A 55 ASP A 66 1 12
HELIX 4 AA4 LEU A 68 SER A 77 1 10
HELIX 5 AA5 GLU A 164 LEU A 173 1 10
HELIX 6 AA6 GLU A 183 THR A 195 1 13
HELIX 7 AA7 LEU A 229 ASP A 234 1 6
HELIX 8 AA8 PRO A 354 PHE A 358 5 5
HELIX 9 AA9 ASP A 430 VAL A 435 5 6
HELIX 10 AB1 HIS A 461 SER A 466 1 6
HELIX 11 AB2 SER A 466 GLY A 471 1 6
HELIX 12 AB3 SER A 478 THR A 485 1 8
HELIX 13 AB4 ASP A 494 ARG A 500 1 7
HELIX 14 AB5 GLU A 538 ALA A 553 1 16
HELIX 15 AB6 THR B 5 HIS B 27 1 23
HELIX 16 AB7 PRO B 28 ALA B 32 5 5
HELIX 17 AB8 MET B 33 LYS B 38 1 6
HELIX 18 AB9 VAL B 46 LEU B 68 1 23
HELIX 19 AC1 GLU B 70 LEU B 79 5 10
HELIX 20 AC2 PRO B 81 THR B 86 5 6
HELIX 21 AC3 ASP B 167 VAL B 171 5 5
HELIX 22 AC4 SER B 188 LYS B 196 1 9
HELIX 23 AC5 ARG B 197 ALA B 199 5 3
HELIX 24 AC6 GLY B 222 GLU B 226 5 5
HELIX 25 AC7 LEU B 229 ALA B 236 1 8
HELIX 26 AC8 LEU B 275 SER B 280 1 6
HELIX 27 AC9 ARG B 324 GLN B 329 1 6
HELIX 28 AD1 GLN B 329 GLU B 334 1 6
HELIX 29 AD2 THR B 356 ARG B 361 1 6
HELIX 30 AD3 ARG B 396 ARG B 401 5 6
HELIX 31 AD4 ARG B 459 VAL B 464 1 6
HELIX 32 AD5 VAL B 464 GLY B 469 1 6
HELIX 33 AD6 SER B 477 ARG B 483 1 7
HELIX 34 AD7 LEU B 486 ASN B 490 5 5
HELIX 35 AD8 ASP B 494 ASP B 502 1 9
HELIX 36 AD9 GLY B 537 PHE B 551 1 15
HELIX 37 AE1 GLN C 9 LEU C 15 1 7
HELIX 38 AE2 GLN C 22 ILE C 26 5 5
HELIX 39 AE3 ASN C 27 GLN C 39 1 13
HELIX 40 AE4 ILE C 45 ALA C 51 5 7
HELIX 41 AE5 PRO C 101 ALA C 109 1 9
HELIX 42 AE6 ARG C 112 ALA C 117 5 6
HELIX 43 AE7 ALA C 118 ASP C 123 1 6
HELIX 44 AE8 ASP C 123 LYS C 139 1 17
HELIX 45 AE9 THR C 154 GLY C 160 1 7
HELIX 46 AF1 ARG C 179 MET C 187 1 9
HELIX 47 AF2 GLU C 195 ALA C 205 1 11
HELIX 48 AF3 THR C 261 LEU C 269 1 9
HELIX 49 AF4 HIS C 273 HIS C 283 1 11
HELIX 50 AF5 MET D 15 ARG D 35 1 21
HELIX 51 AF6 SER D 194 MET D 218 1 25
HELIX 52 AF7 ARG D 225 ALA D 230 1 6
HELIX 53 AF8 SER D 235 SER D 245 1 11
HELIX 54 AF9 ALA D 246 LEU D 254 1 9
HELIX 55 AG1 HIS D 260 LEU D 275 1 16
HELIX 56 AG2 THR D 294 ASN D 297 5 4
HELIX 57 AG3 VAL D 298 GLU D 311 1 14
HELIX 58 AG4 MET E 15 SER E 32 1 18
HELIX 59 AG5 THR E 190 ALA E 193 5 4
HELIX 60 AG6 SER E 194 MET E 218 1 25
HELIX 61 AG7 ARG E 219 ASN E 222 5 4
HELIX 62 AG8 ALA E 232 ALA E 242 1 11
HELIX 63 AG9 ALA E 246 GLU E 252 1 7
HELIX 64 AH1 PRO E 261 THR E 277 1 17
HELIX 65 AH2 THR E 294 ASN E 297 5 4
HELIX 66 AH3 VAL E 298 LEU E 310 1 13
HELIX 67 AH4 MET F 15 VAL F 33 1 19
HELIX 68 AH5 LEU F 53 SER F 57 5 5
HELIX 69 AH6 SER F 194 ASN F 222 1 29
HELIX 70 AH7 ALA F 230 SER F 245 1 16
HELIX 71 AH8 ALA F 246 LEU F 253 1 8
HELIX 72 AH9 HIS F 260 LEU F 275 1 16
HELIX 73 AI1 LEU F 275 LEU F 280 1 6
HELIX 74 AI2 THR F 294 ASN F 297 5 4
HELIX 75 AI3 VAL F 298 LEU F 310 1 13
HELIX 76 AI4 PRO G 16 MET G 36 1 21
HELIX 77 AI5 GLN G 133 GLY G 137 5 5
HELIX 78 AI6 SER G 194 MET G 218 1 25
HELIX 79 AI7 MET G 218 ASN G 223 1 6
HELIX 80 AI8 ALA G 230 SER G 245 1 16
HELIX 81 AI9 ALA G 246 LEU G 253 1 8
HELIX 82 AJ1 HIS G 260 GLY G 273 1 14
HELIX 83 AJ2 THR G 294 ASN G 297 5 4
HELIX 84 AJ3 VAL G 298 GLU G 311 1 14
HELIX 85 AJ4 PRO G 354 CYS G 366 1 13
HELIX 86 AJ5 SER G 411 LEU G 419 1 9
HELIX 87 AJ6 MET H 15 ARG H 35 1 21
HELIX 88 AJ7 LEU H 53 SER H 57 5 5
HELIX 89 AJ8 SER H 194 ARG H 220 1 27
HELIX 90 AJ9 SER H 235 LEU H 254 1 20
HELIX 91 AK1 HIS H 260 LEU H 276 1 17
HELIX 92 AK2 ASN H 283 VAL H 287 5 5
HELIX 93 AK3 VAL H 298 LEU H 310 1 13
HELIX 94 AK4 ASP H 336 GLU H 341 5 6
HELIX 95 AK5 GLY H 355 GLN H 359 5 5
HELIX 96 AK6 ASP H 413 GLY H 422 1 10
HELIX 97 AK7 THR H 428 GLY H 433 5 6
HELIX 98 AK8 MET I 15 ALA I 24 1 10
HELIX 99 AK9 TRP I 25 ARG I 35 1 11
HELIX 100 AL1 SER I 89 SER I 92 5 4
HELIX 101 AL2 SER I 194 ARG I 220 1 27
HELIX 102 AL3 ALA I 230 ASP I 255 1 26
HELIX 103 AL4 HIS I 260 LEU I 276 1 17
HELIX 104 AL5 ASN I 283 VAL I 287 5 5
HELIX 105 AL6 VAL I 298 LEU I 310 1 13
SHEET 1 AA1 3 PRO A 300 ILE A 302 0
SHEET 2 AA1 3 LEU A 90 ALA A 94 -1 N SER A 91 O VAL A 301
SHEET 3 AA1 3 ARG A 443 ASN A 444 -1 O ARG A 443 N ALA A 94
SHEET 1 AA2 3 PHE A 305 LEU A 307 0
SHEET 2 AA2 3 SER A 409 GLY A 414 -1 O GLY A 414 N PHE A 305
SHEET 3 AA2 3 VAL A 338 THR A 342 -1 N ASP A 339 O ARG A 411
SHEET 1 AA3 4 ILE A 504 HIS A 509 0
SHEET 2 AA3 4 ASP A 525 THR A 529 -1 O ASP A 525 N HIS A 509
SHEET 3 AA3 4 LEU A 564 ILE A 567 1 O ILE A 567 N VAL A 528
SHEET 4 AA3 4 ILE A 575 ARG A 576 -1 O ILE A 575 N LEU A 566
SHEET 1 AA4 2 ILE A 512 ARG A 514 0
SHEET 2 AA4 2 LEU A 520 ARG A 522 -1 O LEU A 521 N GLN A 513
SHEET 1 AA5 2 THR B 147 THR B 148 0
SHEET 2 AA5 2 SER B 154 VAL B 155 -1 O VAL B 155 N THR B 147
SHEET 1 AA6 2 LEU B 203 ARG B 204 0
SHEET 2 AA6 2 GLY B 272 PHE B 273 -1 O GLY B 272 N ARG B 204
SHEET 1 AA7 3 PHE B 305 LEU B 307 0
SHEET 2 AA7 3 ARG B 411 ASN B 416 -1 O GLY B 414 N PHE B 305
SHEET 3 AA7 3 TYR B 336 THR B 342 -1 N SER B 337 O THR B 415
SHEET 1 AA8 2 TYR B 374 THR B 376 0
SHEET 2 AA8 2 LEU B 392 GLY B 394 -1 O GLY B 393 N HIS B 375
SHEET 1 AA9 2 LYS B 379 ARG B 380 0
SHEET 2 AA9 2 THR B 388 TRP B 389 -1 O TRP B 389 N LYS B 379
SHEET 1 AB1 2 GLN B 508 HIS B 510 0
SHEET 2 AB1 2 GLY B 523 ASP B 525 -1 O GLY B 523 N HIS B 510
SHEET 1 AB2 2 THR B 565 LEU B 566 0
SHEET 2 AB2 2 CYS B 574 ILE B 575 -1 O CYS B 574 N LEU B 566
SHEET 1 AB3 4 GLN C 209 TRP C 213 0
SHEET 2 AB3 4 LEU C 254 SER C 258 -1 O LEU C 254 N TRP C 213
SHEET 3 AB3 4 ARG C 293 GLU C 299 1 O ARG C 293 N LEU C 255
SHEET 4 AB3 4 ILE C 331 ARG C 337 -1 O SER C 334 N LEU C 296
SHEET 1 AB4 3 VAL D 44 ASP D 50 0
SHEET 2 AB4 3 ASN D 60 ARG D 67 -1 O ILE D 65 N VAL D 45
SHEET 3 AB4 3 LEU D 73 ASP D 75 -1 O ILE D 74 N VAL D 66
SHEET 1 AB5 4 THR D 151 ARG D 153 0
SHEET 2 AB5 4 VAL D 97 PRO D 104 -1 N VAL D 100 O ARG D 153
SHEET 3 AB5 4 TRP D 162 ARG D 172 -1 O LEU D 170 N VAL D 97
SHEET 4 AB5 4 TRP D 178 ARG D 180 -1 O CYS D 179 N VAL D 171
SHEET 1 AB6 2 ARG D 124 GLN D 133 0
SHEET 2 AB6 2 GLN D 140 HIS D 148 -1 O ARG D 147 N TRP D 125
SHEET 1 AB7 3 VAL E 44 PHE E 49 0
SHEET 2 AB7 3 ALA E 61 ARG E 67 -1 O ILE E 65 N ALA E 46
SHEET 3 AB7 3 LEU E 73 ASP E 75 -1 O ILE E 74 N VAL E 66
SHEET 1 AB8 3 THR E 151 MET E 154 0
SHEET 2 AB8 3 VAL E 97 PRO E 104 -1 N VAL E 100 O ARG E 153
SHEET 3 AB8 3 TRP E 162 LEU E 163 -1 O LEU E 163 N LEU E 103
SHEET 1 AB9 4 THR E 151 MET E 154 0
SHEET 2 AB9 4 VAL E 97 PRO E 104 -1 N VAL E 100 O ARG E 153
SHEET 3 AB9 4 PRO E 166 ARG E 172 -1 O VAL E 167 N ILE E 99
SHEET 4 AB9 4 TRP E 178 ARG E 180 -1 O CYS E 179 N VAL E 171
SHEET 1 AC1 2 LYS E 126 GLN E 133 0
SHEET 2 AC1 2 GLN E 140 ARG E 147 -1 O VAL E 145 N GLU E 128
SHEET 1 AC2 3 VAL F 44 PHE F 49 0
SHEET 2 AC2 3 ALA F 61 PHE F 68 -1 O THR F 62 N GLU F 48
SHEET 3 AC2 3 THR F 72 ASP F 75 -1 O ILE F 74 N VAL F 66
SHEET 1 AC3 2 GLU F 128 VAL F 132 0
SHEET 2 AC3 2 SER F 141 VAL F 145 -1 O SER F 141 N VAL F 132
SHEET 1 AC4 2 LEU F 170 ARG F 172 0
SHEET 2 AC4 2 TRP F 178 ARG F 180 -1 O CYS F 179 N VAL F 171
SHEET 1 AC5 3 VAL G 44 PHE G 49 0
SHEET 2 AC5 3 ALA G 61 ARG G 67 -1 O ILE G 65 N ALA G 46
SHEET 3 AC5 3 LEU G 73 ASP G 75 -1 O ILE G 74 N VAL G 66
SHEET 1 AC6 2 ARG G 58 LEU G 59 0
SHEET 2 AC6 2 CYS G 86 ASP G 87 -1 O CYS G 86 N LEU G 59
SHEET 1 AC7 4 THR G 151 MET G 154 0
SHEET 2 AC7 4 ASP G 98 ALA G 102 -1 N VAL G 100 O ARG G 153
SHEET 3 AC7 4 CYS G 165 ARG G 172 -1 O VAL G 167 N ILE G 99
SHEET 4 AC7 4 TRP G 178 ARG G 180 -1 O CYS G 179 N VAL G 171
SHEET 1 AC8 2 TRP G 125 ASN G 131 0
SHEET 2 AC8 2 GLU G 142 ARG G 147 -1 O VAL G 143 N VAL G 130
SHEET 1 AC9 5 VAL G 318 ILE G 321 0
SHEET 2 AC9 5 VAL G 435 VAL G 442 -1 O LEU G 439 N ILE G 321
SHEET 3 AC9 5 PHE G 345 SER G 351 -1 N ARG G 350 O LYS G 436
SHEET 4 AC9 5 TYR G 405 LEU G 410 -1 O PHE G 406 N LEU G 347
SHEET 5 AC9 5 VAL G 385 PRO G 389 -1 N ARG G 388 O ALA G 407
SHEET 1 AD1 4 GLN G 325 LYS G 326 0
SHEET 2 AD1 4 MET G 329 ALA G 333 -1 O MET G 329 N LYS G 326
SHEET 3 AD1 4 ARG G 423 TYR G 427 -1 O CYS G 424 N GLY G 332
SHEET 4 AD1 4 LYS G 367 GLY G 369 -1 N LYS G 367 O TYR G 427
SHEET 1 AD2 2 VAL H 44 PHE H 49 0
SHEET 2 AD2 2 ALA H 61 VAL H 66 -1 O ILE H 65 N VAL H 45
SHEET 1 AD3 4 THR H 151 MET H 154 0
SHEET 2 AD3 4 LEU H 96 ALA H 102 -1 N ALA H 102 O THR H 151
SHEET 3 AD3 4 CYS H 165 ARG H 172 -1 O LEU H 170 N VAL H 97
SHEET 4 AD3 4 TRP H 178 ARG H 180 -1 O CYS H 179 N VAL H 171
SHEET 1 AD4 2 TRP H 125 VAL H 132 0
SHEET 2 AD4 2 SER H 141 HIS H 148 -1 O VAL H 145 N GLU H 128
SHEET 1 AD5 2 GLU H 331 GLY H 332 0
SHEET 2 AD5 2 CYS H 424 THR H 425 -1 O CYS H 424 N GLY H 332
SHEET 1 AD6 2 PRO H 389 VAL H 390 0
SHEET 2 AD6 2 TYR H 405 PHE H 406 -1 O TYR H 405 N VAL H 390
SHEET 1 AD7 3 VAL I 44 ASP I 50 0
SHEET 2 AD7 3 ARG I 58 PHE I 68 -1 O ARG I 63 N GLU I 48
SHEET 3 AD7 3 THR I 72 ASP I 75 -1 O THR I 72 N PHE I 68
SHEET 1 AD8 3 VAL I 44 ASP I 50 0
SHEET 2 AD8 3 ARG I 58 PHE I 68 -1 O ARG I 63 N GLU I 48
SHEET 3 AD8 3 CYS I 86 ASP I 87 -1 O CYS I 86 N LEU I 59
SHEET 1 AD9 4 THR I 151 ARG I 153 0
SHEET 2 AD9 4 VAL I 97 PRO I 104 -1 N VAL I 100 O ARG I 153
SHEET 3 AD9 4 TRP I 162 ARG I 172 -1 O LEU I 163 N LEU I 103
SHEET 4 AD9 4 TRP I 178 ARG I 180 -1 O CYS I 179 N VAL I 171
SHEET 1 AE1 2 LYS I 126 VAL I 130 0
SHEET 2 AE1 2 VAL I 143 ARG I 147 -1 O VAL I 145 N GLU I 128
CISPEP 1 ILE B 313 ILE B 314 0 -1.17
CISPEP 2 GLY B 484 THR B 485 0 -1.59
CISPEP 3 ALA C 109 GLN C 110 0 13.96
CISPEP 4 ARG C 192 THR C 193 0 -3.55
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
