GenomeNet

Database: PDB
Entry: 6GJ3
LinkDB: 6GJ3
Original site: 6GJ3 
HEADER    STRUCTURAL PROTEIN                      15-MAY-18   6GJ3              
TITLE     THE BASEPLATE COMPLEX FROM THE TYPE VI SECRETION SYSTEM               
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TSSG;                                                      
COMPND   3 CHAIN: C;                                                            
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: TSSK;                                                      
COMPND   7 CHAIN: D, E, F, G, H, I;                                             
COMPND   8 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   3 ORGANISM_TAXID: 562;                                                 
SOURCE   4 GENE: IMPH, ECDEC6A_3652;                                            
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 MOL_ID: 2;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   9 ORGANISM_TAXID: 562;                                                 
SOURCE  10 GENE: ECDEC6A_3669;                                                  
SOURCE  11 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  12 EXPRESSION_SYSTEM_TAXID: 469008                                      
KEYWDS    SECRETION, BASEPLATE, COMPLEX, STRUCTURAL PROTEIN                     
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    C.RAPISARDA,R.FRONZES                                                 
REVDAT   4   18-DEC-19 6GJ3    1       SCALE                                    
REVDAT   3   05-DEC-18 6GJ3    1       JRNL                                     
REVDAT   2   24-OCT-18 6GJ3    1       JRNL                                     
REVDAT   1   17-OCT-18 6GJ3    0                                                
JRNL        AUTH   Y.CHERRAK,C.RAPISARDA,R.PELLARIN,G.BOUVIER,B.BARDIAUX,       
JRNL        AUTH 2 F.ALLAIN,C.MALOSSE,M.REY,J.CHAMOT-ROOKE,E.CASCALES,          
JRNL        AUTH 3 R.FRONZES,E.DURAND                                           
JRNL        TITL   BIOGENESIS AND STRUCTURE OF A TYPE VI SECRETION BASEPLATE.   
JRNL        REF    NAT MICROBIOL                 V.   3  1404 2018              
JRNL        REFN                   ESSN 2058-5276                               
JRNL        PMID   30323254                                                     
JRNL        DOI    10.1038/S41564-018-0260-1                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    4.30 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : EPU, GCTF, UCSF CHIMERA, COOT,            
REMARK   3                            CRYOSPARC, RELION, RELION, PHENIX         
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : NULL                                
REMARK   3   REFINEMENT SPACE             : REAL                                
REMARK   3   REFINEMENT PROTOCOL          : RIGID BODY FIT                      
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : NULL                                             
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 4.300                          
REMARK   3   NUMBER OF PARTICLES               : 32504                          
REMARK   3   CTF CORRECTION METHOD             : PHASE FLIPPING AND AMPLITUDE   
REMARK   3                                       CORRECTION                     
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 6GJ3 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 15-MAY-18.                  
REMARK 100 THE DEPOSITION ID IS D_1200010050.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : NULL                              
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : COMPLEX OF TSSK, TSSF AND TSSG,   
REMARK 245                                    COMPONENTS OF THE TYPE VI         
REMARK 245                                    SECRETION SYSTEM BASEPLATE        
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 0.20                              
REMARK 245   SAMPLE SUPPORT DETAILS         : NULL                              
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : NULL                           
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : NULL                           
REMARK 245   MICROSCOPE MODEL                  : FEI TITAN KRIOS                
REMARK 245   DETECTOR TYPE                     : GATAN K2 SUMMIT (4K X 4K)      
REMARK 245   MINIMUM DEFOCUS (NM)              : 700.00                         
REMARK 245   MAXIMUM DEFOCUS (NM)              : 2000.00                        
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : 2.70                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 1.50                           
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : 130000                         
REMARK 245   CALIBRATED MAGNIFICATION          : NULL                           
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 300                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: HEPTAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: HEPTAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 39800 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 103710 ANGSTROM**2                      
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -211.0 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D, E, F, G, H, I                   
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET C   100                                                      
REMARK 465     PRO C   101                                                      
REMARK 465     ALA C   102                                                      
REMARK 465     HIS C   103                                                      
REMARK 465     TYR C   104                                                      
REMARK 465     ILE C   105                                                      
REMARK 465     SER C   106                                                      
REMARK 465     ASP C   107                                                      
REMARK 465     ILE C   108                                                      
REMARK 465     ALA C   109                                                      
REMARK 465     GLN C   110                                                      
REMARK 465     GLN C   111                                                      
REMARK 465     ARG C   112                                                      
REMARK 465     GLU C   113                                                      
REMARK 465     GLY C   114                                                      
REMARK 465     HIS C   115                                                      
REMARK 465     GLU C   116                                                      
REMARK 465     ALA C   117                                                      
REMARK 465     ALA C   118                                                      
REMARK 465     ALA C   119                                                      
REMARK 465     ASP C   120                                                      
REMARK 465     PHE C   121                                                      
REMARK 465     LEU C   122                                                      
REMARK 465     ASP C   123                                                      
REMARK 465     ILE C   124                                                      
REMARK 465     PHE C   125                                                      
REMARK 465     SER C   126                                                      
REMARK 465     HIS C   127                                                      
REMARK 465     ARG C   128                                                      
REMARK 465     LEU C   129                                                      
REMARK 465     ILE C   130                                                      
REMARK 465     THR C   131                                                      
REMARK 465     GLN C   132                                                      
REMARK 465     TYR C   133                                                      
REMARK 465     TYR C   134                                                      
REMARK 465     ARG C   135                                                      
REMARK 465     ILE C   136                                                      
REMARK 465     TRP C   137                                                      
REMARK 465     ARG C   138                                                      
REMARK 465     LYS C   139                                                      
REMARK 465     TYR C   140                                                      
REMARK 465     SER C   141                                                      
REMARK 465     TYR C   142                                                      
REMARK 465     PRO C   143                                                      
REMARK 465     ALA C   144                                                      
REMARK 465     THR C   145                                                      
REMARK 465     PHE C   146                                                      
REMARK 465     GLU C   147                                                      
REMARK 465     ALA C   148                                                      
REMARK 465     GLY C   149                                                      
REMARK 465     GLY C   150                                                      
REMARK 465     GLN C   151                                                      
REMARK 465     ASP C   152                                                      
REMARK 465     LYS C   153                                                      
REMARK 465     THR C   154                                                      
REMARK 465     SER C   155                                                      
REMARK 465     GLN C   156                                                      
REMARK 465     TYR C   157                                                      
REMARK 465     LEU C   158                                                      
REMARK 465     LEU C   159                                                      
REMARK 465     GLY C   160                                                      
REMARK 465     LEU C   161                                                      
REMARK 465     ALA C   162                                                      
REMARK 465     ARG C   163                                                      
REMARK 465     LEU C   164                                                      
REMARK 465     GLY C   165                                                      
REMARK 465     ILE C   166                                                      
REMARK 465     PRO C   167                                                      
REMARK 465     GLY C   168                                                      
REMARK 465     CYS C   169                                                      
REMARK 465     ALA C   170                                                      
REMARK 465     GLN C   171                                                      
REMARK 465     ASN C   172                                                      
REMARK 465     ILE C   173                                                      
REMARK 465     ALA C   174                                                      
REMARK 465     THR C   175                                                      
REMARK 465     PRO C   176                                                      
REMARK 465     VAL C   177                                                      
REMARK 465     SER C   178                                                      
REMARK 465     ARG C   179                                                      
REMARK 465     PHE C   180                                                      
REMARK 465     LEU C   181                                                      
REMARK 465     ALA C   182                                                      
REMARK 465     LEU C   183                                                      
REMARK 465     LEU C   184                                                      
REMARK 465     PRO C   185                                                      
REMARK 465     LEU C   186                                                      
REMARK 465     MET C   187                                                      
REMARK 465     LEU C   188                                                      
REMARK 465     LEU C   189                                                      
REMARK 465     PRO C   190                                                      
REMARK 465     GLY C   191                                                      
REMARK 465     ARG C   192                                                      
REMARK 465     THR C   193                                                      
REMARK 465     ALA C   194                                                      
REMARK 465     GLU C   195                                                      
REMARK 465     GLY C   196                                                      
REMARK 465     LEU C   197                                                      
REMARK 465     THR C   198                                                      
REMARK 465     SER C   199                                                      
REMARK 465     LEU C   200                                                      
REMARK 465     VAL C   201                                                      
REMARK 465     THR C   202                                                      
REMARK 465     LEU C   203                                                      
REMARK 465     LEU C   204                                                      
REMARK 465     ALA C   205                                                      
REMARK 465     PRO C   206                                                      
REMARK 465     GLY C   207                                                      
REMARK 465     THR C   208                                                      
REMARK 465     GLN C   209                                                      
REMARK 465     ALA C   210                                                      
REMARK 465     ARG C   211                                                      
REMARK 465     VAL C   212                                                      
REMARK 465     TRP C   213                                                      
REMARK 465     HIS C   214                                                      
REMARK 465     GLY C   251                                                      
REMARK 465     GLN C   252                                                      
REMARK 465     VAL C   253                                                      
REMARK 465     LEU C   254                                                      
REMARK 465     LEU C   255                                                      
REMARK 465     GLN C   256                                                      
REMARK 465     LEU C   257                                                      
REMARK 465     SER C   258                                                      
REMARK 465     THR C   259                                                      
REMARK 465     GLN C   260                                                      
REMARK 465     THR C   261                                                      
REMARK 465     GLY C   262                                                      
REMARK 465     SER C   263                                                      
REMARK 465     GLU C   264                                                      
REMARK 465     VAL C   265                                                      
REMARK 465     GLN C   266                                                      
REMARK 465     GLY C   267                                                      
REMARK 465     TRP C   268                                                      
REMARK 465     LEU C   269                                                      
REMARK 465     PRO C   270                                                      
REMARK 465     GLY C   271                                                      
REMARK 465     GLY C   272                                                      
REMARK 465     HIS C   273                                                      
REMARK 465     LEU C   274                                                      
REMARK 465     TYR C   275                                                      
REMARK 465     SER C   276                                                      
REMARK 465     ASP C   277                                                      
REMARK 465     LEU C   278                                                      
REMARK 465     LEU C   279                                                      
REMARK 465     ALA C   280                                                      
REMARK 465     LEU C   281                                                      
REMARK 465     LEU C   282                                                      
REMARK 465     HIS C   283                                                      
REMARK 465     VAL C   284                                                      
REMARK 465     TYR C   285                                                      
REMARK 465     LEU C   286                                                      
REMARK 465     GLY C   287                                                      
REMARK 465     SER C   288                                                      
REMARK 465     ARG C   289                                                      
REMARK 465     LEU C   290                                                      
REMARK 465     ASP C   291                                                      
REMARK 465     VAL C   292                                                      
REMARK 465     ARG C   293                                                      
REMARK 465     LEU C   294                                                      
REMARK 465     GLN C   295                                                      
REMARK 465     LEU C   296                                                      
REMARK 465     CYS C   297                                                      
REMARK 465     VAL C   298                                                      
REMARK 465     GLU C   299                                                      
REMARK 465     ARG C   300                                                      
REMARK 465     THR C   327                                                      
REMARK 465     GLN C   328                                                      
REMARK 465     ALA C   329                                                      
REMARK 465     LYS C   330                                                      
REMARK 465     ILE C   331                                                      
REMARK 465     THR C   332                                                      
REMARK 465     THR C   333                                                      
REMARK 465     SER C   334                                                      
REMARK 465     ALA C   335                                                      
REMARK 465     ALA C   336                                                      
REMARK 465     ARG C   337                                                      
REMARK 465     VAL C   338                                                      
REMARK 465     MET C   339                                                      
REMARK 465     THR C   340                                                      
REMARK 465     ILE C   341                                                      
REMARK 465     SER C   342                                                      
REMARK 465     LEU C   343                                                      
REMARK 465     GLY C   344                                                      
REMARK 465     ARG C   345                                                      
REMARK 465     TYR C   346                                                      
REMARK 465     GLN C   347                                                      
REMARK 465     ARG C   348                                                      
REMARK 465     VAL C   349                                                      
REMARK 465     GLN C   350                                                      
REMARK 465     GLU C   351                                                      
REMARK 465     HIS C   352                                                      
REMARK 465     TYR C   353                                                      
REMARK 465     GLN C   354                                                      
REMARK 465     ARG C   355                                                      
REMARK 465     LYS C   356                                                      
REMARK 465     GLU C   357                                                      
REMARK 465     THR C   358                                                      
REMARK 465     GLN C   359                                                      
REMARK 465     GLU C   360                                                      
REMARK 465     ASN C   361                                                      
REMARK 465     GLY C   362                                                      
REMARK 465     ASP C   363                                                      
REMARK 465     TYR C   364                                                      
REMARK 465     ARG C   365                                                      
REMARK 465     TRP C   366                                                      
REMARK 465     ALA D   312                                                      
REMARK 465     SER D   313                                                      
REMARK 465     LEU D   314                                                      
REMARK 465     PRO D   315                                                      
REMARK 465     SER D   316                                                      
REMARK 465     ARG D   317                                                      
REMARK 465     VAL D   318                                                      
REMARK 465     VAL D   319                                                      
REMARK 465     PHE D   320                                                      
REMARK 465     ILE D   321                                                      
REMARK 465     GLU D   322                                                      
REMARK 465     LEU D   323                                                      
REMARK 465     LYS D   324                                                      
REMARK 465     GLN D   325                                                      
REMARK 465     LYS D   326                                                      
REMARK 465     GLY D   327                                                      
REMARK 465     VAL D   328                                                      
REMARK 465     MET D   329                                                      
REMARK 465     TRP D   330                                                      
REMARK 465     GLU D   331                                                      
REMARK 465     GLY D   332                                                      
REMARK 465     ALA D   333                                                      
REMARK 465     LEU D   334                                                      
REMARK 465     HIS D   335                                                      
REMARK 465     ASP D   336                                                      
REMARK 465     ALA D   337                                                      
REMARK 465     ARG D   338                                                      
REMARK 465     LEU D   339                                                      
REMARK 465     ARG D   340                                                      
REMARK 465     GLU D   341                                                      
REMARK 465     GLY D   342                                                      
REMARK 465     ALA D   343                                                      
REMARK 465     ASP D   344                                                      
REMARK 465     PHE D   345                                                      
REMARK 465     TRP D   346                                                      
REMARK 465     LEU D   347                                                      
REMARK 465     SER D   348                                                      
REMARK 465     VAL D   349                                                      
REMARK 465     ARG D   350                                                      
REMARK 465     SER D   351                                                      
REMARK 465     SER D   352                                                      
REMARK 465     MET D   353                                                      
REMARK 465     PRO D   354                                                      
REMARK 465     GLY D   355                                                      
REMARK 465     HIS D   356                                                      
REMARK 465     GLU D   357                                                      
REMARK 465     LEU D   358                                                      
REMARK 465     GLN D   359                                                      
REMARK 465     THR D   360                                                      
REMARK 465     LYS D   361                                                      
REMARK 465     PHE D   362                                                      
REMARK 465     PRO D   363                                                      
REMARK 465     GLN D   364                                                      
REMARK 465     LEU D   365                                                      
REMARK 465     CYS D   366                                                      
REMARK 465     LYS D   367                                                      
REMARK 465     ALA D   368                                                      
REMARK 465     GLY D   369                                                      
REMARK 465     SER D   370                                                      
REMARK 465     PRO D   371                                                      
REMARK 465     ASP D   372                                                      
REMARK 465     ASP D   373                                                      
REMARK 465     VAL D   374                                                      
REMARK 465     SER D   375                                                      
REMARK 465     GLU D   376                                                      
REMARK 465     VAL D   377                                                      
REMARK 465     VAL D   378                                                      
REMARK 465     ASN D   379                                                      
REMARK 465     VAL D   380                                                      
REMARK 465     ALA D   381                                                      
REMARK 465     LEU D   382                                                      
REMARK 465     SER D   383                                                      
REMARK 465     GLY D   384                                                      
REMARK 465     VAL D   385                                                      
REMARK 465     ILE D   386                                                      
REMARK 465     ILE D   387                                                      
REMARK 465     ARG D   388                                                      
REMARK 465     PRO D   389                                                      
REMARK 465     VAL D   390                                                      
REMARK 465     THR D   391                                                      
REMARK 465     HIS D   392                                                      
REMARK 465     VAL D   393                                                      
REMARK 465     PRO D   394                                                      
REMARK 465     ALA D   395                                                      
REMARK 465     ALA D   396                                                      
REMARK 465     ILE D   397                                                      
REMARK 465     PRO D   398                                                      
REMARK 465     LEU D   399                                                      
REMARK 465     ARG D   400                                                      
REMARK 465     LEU D   401                                                      
REMARK 465     GLU D   402                                                      
REMARK 465     ASN D   403                                                      
REMARK 465     GLN D   404                                                      
REMARK 465     TYR D   405                                                      
REMARK 465     PHE D   406                                                      
REMARK 465     ALA D   407                                                      
REMARK 465     LEU D   408                                                      
REMARK 465     ASP D   409                                                      
REMARK 465     LEU D   410                                                      
REMARK 465     SER D   411                                                      
REMARK 465     THR D   412                                                      
REMARK 465     ASP D   413                                                      
REMARK 465     ALA D   414                                                      
REMARK 465     ALA D   415                                                      
REMARK 465     ARG D   416                                                      
REMARK 465     ALA D   417                                                      
REMARK 465     MET D   418                                                      
REMARK 465     LEU D   419                                                      
REMARK 465     ASP D   420                                                      
REMARK 465     ALA D   421                                                      
REMARK 465     GLY D   422                                                      
REMARK 465     ARG D   423                                                      
REMARK 465     CYS D   424                                                      
REMARK 465     THR D   425                                                      
REMARK 465     PHE D   426                                                      
REMARK 465     TYR D   427                                                      
REMARK 465     THR D   428                                                      
REMARK 465     PRO D   429                                                      
REMARK 465     ALA D   430                                                      
REMARK 465     SER D   431                                                      
REMARK 465     LEU D   432                                                      
REMARK 465     GLY D   433                                                      
REMARK 465     ASP D   434                                                      
REMARK 465     VAL D   435                                                      
REMARK 465     LYS D   436                                                      
REMARK 465     LEU D   437                                                      
REMARK 465     GLU D   438                                                      
REMARK 465     LEU D   439                                                      
REMARK 465     PHE D   440                                                      
REMARK 465     ALA D   441                                                      
REMARK 465     VAL D   442                                                      
REMARK 465     LEU D   443                                                      
REMARK 465     ARG D   444                                                      
REMARK 465     THR D   445                                                      
REMARK 465     ALA E   312                                                      
REMARK 465     SER E   313                                                      
REMARK 465     LEU E   314                                                      
REMARK 465     PRO E   315                                                      
REMARK 465     SER E   316                                                      
REMARK 465     ARG E   317                                                      
REMARK 465     VAL E   318                                                      
REMARK 465     VAL E   319                                                      
REMARK 465     PHE E   320                                                      
REMARK 465     ILE E   321                                                      
REMARK 465     GLU E   322                                                      
REMARK 465     LEU E   323                                                      
REMARK 465     LYS E   324                                                      
REMARK 465     GLN E   325                                                      
REMARK 465     LYS E   326                                                      
REMARK 465     GLY E   327                                                      
REMARK 465     VAL E   328                                                      
REMARK 465     MET E   329                                                      
REMARK 465     TRP E   330                                                      
REMARK 465     GLU E   331                                                      
REMARK 465     GLY E   332                                                      
REMARK 465     ALA E   333                                                      
REMARK 465     LEU E   334                                                      
REMARK 465     HIS E   335                                                      
REMARK 465     ASP E   336                                                      
REMARK 465     ALA E   337                                                      
REMARK 465     ARG E   338                                                      
REMARK 465     LEU E   339                                                      
REMARK 465     ARG E   340                                                      
REMARK 465     GLU E   341                                                      
REMARK 465     GLY E   342                                                      
REMARK 465     ALA E   343                                                      
REMARK 465     ASP E   344                                                      
REMARK 465     PHE E   345                                                      
REMARK 465     TRP E   346                                                      
REMARK 465     LEU E   347                                                      
REMARK 465     SER E   348                                                      
REMARK 465     VAL E   349                                                      
REMARK 465     ARG E   350                                                      
REMARK 465     SER E   351                                                      
REMARK 465     SER E   352                                                      
REMARK 465     MET E   353                                                      
REMARK 465     PRO E   354                                                      
REMARK 465     GLY E   355                                                      
REMARK 465     HIS E   356                                                      
REMARK 465     GLU E   357                                                      
REMARK 465     LEU E   358                                                      
REMARK 465     GLN E   359                                                      
REMARK 465     THR E   360                                                      
REMARK 465     LYS E   361                                                      
REMARK 465     PHE E   362                                                      
REMARK 465     PRO E   363                                                      
REMARK 465     GLN E   364                                                      
REMARK 465     LEU E   365                                                      
REMARK 465     CYS E   366                                                      
REMARK 465     LYS E   367                                                      
REMARK 465     ALA E   368                                                      
REMARK 465     GLY E   369                                                      
REMARK 465     SER E   370                                                      
REMARK 465     PRO E   371                                                      
REMARK 465     ASP E   372                                                      
REMARK 465     ASP E   373                                                      
REMARK 465     VAL E   374                                                      
REMARK 465     SER E   375                                                      
REMARK 465     GLU E   376                                                      
REMARK 465     VAL E   377                                                      
REMARK 465     VAL E   378                                                      
REMARK 465     ASN E   379                                                      
REMARK 465     VAL E   380                                                      
REMARK 465     ALA E   381                                                      
REMARK 465     LEU E   382                                                      
REMARK 465     SER E   383                                                      
REMARK 465     GLY E   384                                                      
REMARK 465     VAL E   385                                                      
REMARK 465     ILE E   386                                                      
REMARK 465     ILE E   387                                                      
REMARK 465     ARG E   388                                                      
REMARK 465     PRO E   389                                                      
REMARK 465     VAL E   390                                                      
REMARK 465     THR E   391                                                      
REMARK 465     HIS E   392                                                      
REMARK 465     VAL E   393                                                      
REMARK 465     PRO E   394                                                      
REMARK 465     ALA E   395                                                      
REMARK 465     ALA E   396                                                      
REMARK 465     ILE E   397                                                      
REMARK 465     PRO E   398                                                      
REMARK 465     LEU E   399                                                      
REMARK 465     ARG E   400                                                      
REMARK 465     LEU E   401                                                      
REMARK 465     GLU E   402                                                      
REMARK 465     ASN E   403                                                      
REMARK 465     GLN E   404                                                      
REMARK 465     TYR E   405                                                      
REMARK 465     PHE E   406                                                      
REMARK 465     ALA E   407                                                      
REMARK 465     LEU E   408                                                      
REMARK 465     ASP E   409                                                      
REMARK 465     LEU E   410                                                      
REMARK 465     SER E   411                                                      
REMARK 465     THR E   412                                                      
REMARK 465     ASP E   413                                                      
REMARK 465     ALA E   414                                                      
REMARK 465     ALA E   415                                                      
REMARK 465     ARG E   416                                                      
REMARK 465     ALA E   417                                                      
REMARK 465     MET E   418                                                      
REMARK 465     LEU E   419                                                      
REMARK 465     ASP E   420                                                      
REMARK 465     ALA E   421                                                      
REMARK 465     GLY E   422                                                      
REMARK 465     ARG E   423                                                      
REMARK 465     CYS E   424                                                      
REMARK 465     THR E   425                                                      
REMARK 465     PHE E   426                                                      
REMARK 465     TYR E   427                                                      
REMARK 465     THR E   428                                                      
REMARK 465     PRO E   429                                                      
REMARK 465     ALA E   430                                                      
REMARK 465     SER E   431                                                      
REMARK 465     LEU E   432                                                      
REMARK 465     GLY E   433                                                      
REMARK 465     ASP E   434                                                      
REMARK 465     VAL E   435                                                      
REMARK 465     LYS E   436                                                      
REMARK 465     LEU E   437                                                      
REMARK 465     GLU E   438                                                      
REMARK 465     LEU E   439                                                      
REMARK 465     PHE E   440                                                      
REMARK 465     ALA E   441                                                      
REMARK 465     VAL E   442                                                      
REMARK 465     LEU E   443                                                      
REMARK 465     ARG E   444                                                      
REMARK 465     THR E   445                                                      
REMARK 465     ALA F   312                                                      
REMARK 465     SER F   313                                                      
REMARK 465     LEU F   314                                                      
REMARK 465     PRO F   315                                                      
REMARK 465     SER F   316                                                      
REMARK 465     ARG F   317                                                      
REMARK 465     VAL F   318                                                      
REMARK 465     VAL F   319                                                      
REMARK 465     PHE F   320                                                      
REMARK 465     ILE F   321                                                      
REMARK 465     GLU F   322                                                      
REMARK 465     LEU F   323                                                      
REMARK 465     LYS F   324                                                      
REMARK 465     GLN F   325                                                      
REMARK 465     LYS F   326                                                      
REMARK 465     GLY F   327                                                      
REMARK 465     VAL F   328                                                      
REMARK 465     MET F   329                                                      
REMARK 465     TRP F   330                                                      
REMARK 465     GLU F   331                                                      
REMARK 465     GLY F   332                                                      
REMARK 465     ALA F   333                                                      
REMARK 465     LEU F   334                                                      
REMARK 465     HIS F   335                                                      
REMARK 465     ASP F   336                                                      
REMARK 465     ALA F   337                                                      
REMARK 465     ARG F   338                                                      
REMARK 465     LEU F   339                                                      
REMARK 465     ARG F   340                                                      
REMARK 465     GLU F   341                                                      
REMARK 465     GLY F   342                                                      
REMARK 465     ALA F   343                                                      
REMARK 465     ASP F   344                                                      
REMARK 465     PHE F   345                                                      
REMARK 465     TRP F   346                                                      
REMARK 465     LEU F   347                                                      
REMARK 465     SER F   348                                                      
REMARK 465     VAL F   349                                                      
REMARK 465     ARG F   350                                                      
REMARK 465     SER F   351                                                      
REMARK 465     SER F   352                                                      
REMARK 465     MET F   353                                                      
REMARK 465     PRO F   354                                                      
REMARK 465     GLY F   355                                                      
REMARK 465     HIS F   356                                                      
REMARK 465     GLU F   357                                                      
REMARK 465     LEU F   358                                                      
REMARK 465     GLN F   359                                                      
REMARK 465     THR F   360                                                      
REMARK 465     LYS F   361                                                      
REMARK 465     PHE F   362                                                      
REMARK 465     PRO F   363                                                      
REMARK 465     GLN F   364                                                      
REMARK 465     LEU F   365                                                      
REMARK 465     CYS F   366                                                      
REMARK 465     LYS F   367                                                      
REMARK 465     ALA F   368                                                      
REMARK 465     GLY F   369                                                      
REMARK 465     SER F   370                                                      
REMARK 465     PRO F   371                                                      
REMARK 465     ASP F   372                                                      
REMARK 465     ASP F   373                                                      
REMARK 465     VAL F   374                                                      
REMARK 465     SER F   375                                                      
REMARK 465     GLU F   376                                                      
REMARK 465     VAL F   377                                                      
REMARK 465     VAL F   378                                                      
REMARK 465     ASN F   379                                                      
REMARK 465     VAL F   380                                                      
REMARK 465     ALA F   381                                                      
REMARK 465     LEU F   382                                                      
REMARK 465     SER F   383                                                      
REMARK 465     GLY F   384                                                      
REMARK 465     VAL F   385                                                      
REMARK 465     ILE F   386                                                      
REMARK 465     ILE F   387                                                      
REMARK 465     ARG F   388                                                      
REMARK 465     PRO F   389                                                      
REMARK 465     VAL F   390                                                      
REMARK 465     THR F   391                                                      
REMARK 465     HIS F   392                                                      
REMARK 465     VAL F   393                                                      
REMARK 465     PRO F   394                                                      
REMARK 465     ALA F   395                                                      
REMARK 465     ALA F   396                                                      
REMARK 465     ILE F   397                                                      
REMARK 465     PRO F   398                                                      
REMARK 465     LEU F   399                                                      
REMARK 465     ARG F   400                                                      
REMARK 465     LEU F   401                                                      
REMARK 465     GLU F   402                                                      
REMARK 465     ASN F   403                                                      
REMARK 465     GLN F   404                                                      
REMARK 465     TYR F   405                                                      
REMARK 465     PHE F   406                                                      
REMARK 465     ALA F   407                                                      
REMARK 465     LEU F   408                                                      
REMARK 465     ASP F   409                                                      
REMARK 465     LEU F   410                                                      
REMARK 465     SER F   411                                                      
REMARK 465     THR F   412                                                      
REMARK 465     ASP F   413                                                      
REMARK 465     ALA F   414                                                      
REMARK 465     ALA F   415                                                      
REMARK 465     ARG F   416                                                      
REMARK 465     ALA F   417                                                      
REMARK 465     MET F   418                                                      
REMARK 465     LEU F   419                                                      
REMARK 465     ASP F   420                                                      
REMARK 465     ALA F   421                                                      
REMARK 465     GLY F   422                                                      
REMARK 465     ARG F   423                                                      
REMARK 465     CYS F   424                                                      
REMARK 465     THR F   425                                                      
REMARK 465     PHE F   426                                                      
REMARK 465     TYR F   427                                                      
REMARK 465     THR F   428                                                      
REMARK 465     PRO F   429                                                      
REMARK 465     ALA F   430                                                      
REMARK 465     SER F   431                                                      
REMARK 465     LEU F   432                                                      
REMARK 465     GLY F   433                                                      
REMARK 465     ASP F   434                                                      
REMARK 465     VAL F   435                                                      
REMARK 465     LYS F   436                                                      
REMARK 465     LEU F   437                                                      
REMARK 465     GLU F   438                                                      
REMARK 465     LEU F   439                                                      
REMARK 465     PHE F   440                                                      
REMARK 465     ALA F   441                                                      
REMARK 465     VAL F   442                                                      
REMARK 465     LEU F   443                                                      
REMARK 465     ARG F   444                                                      
REMARK 465     THR F   445                                                      
REMARK 465     THR G   445                                                      
REMARK 465     THR H   445                                                      
REMARK 465     ALA I   312                                                      
REMARK 465     SER I   313                                                      
REMARK 465     LEU I   314                                                      
REMARK 465     PRO I   315                                                      
REMARK 465     SER I   316                                                      
REMARK 465     ARG I   317                                                      
REMARK 465     VAL I   318                                                      
REMARK 465     VAL I   319                                                      
REMARK 465     PHE I   320                                                      
REMARK 465     ILE I   321                                                      
REMARK 465     GLU I   322                                                      
REMARK 465     LEU I   323                                                      
REMARK 465     LYS I   324                                                      
REMARK 465     GLN I   325                                                      
REMARK 465     LYS I   326                                                      
REMARK 465     GLY I   327                                                      
REMARK 465     VAL I   328                                                      
REMARK 465     MET I   329                                                      
REMARK 465     TRP I   330                                                      
REMARK 465     GLU I   331                                                      
REMARK 465     GLY I   332                                                      
REMARK 465     ALA I   333                                                      
REMARK 465     LEU I   334                                                      
REMARK 465     HIS I   335                                                      
REMARK 465     ASP I   336                                                      
REMARK 465     ALA I   337                                                      
REMARK 465     ARG I   338                                                      
REMARK 465     LEU I   339                                                      
REMARK 465     ARG I   340                                                      
REMARK 465     GLU I   341                                                      
REMARK 465     GLY I   342                                                      
REMARK 465     ALA I   343                                                      
REMARK 465     ASP I   344                                                      
REMARK 465     PHE I   345                                                      
REMARK 465     TRP I   346                                                      
REMARK 465     LEU I   347                                                      
REMARK 465     SER I   348                                                      
REMARK 465     VAL I   349                                                      
REMARK 465     ARG I   350                                                      
REMARK 465     SER I   351                                                      
REMARK 465     SER I   352                                                      
REMARK 465     MET I   353                                                      
REMARK 465     PRO I   354                                                      
REMARK 465     GLY I   355                                                      
REMARK 465     HIS I   356                                                      
REMARK 465     GLU I   357                                                      
REMARK 465     LEU I   358                                                      
REMARK 465     GLN I   359                                                      
REMARK 465     THR I   360                                                      
REMARK 465     LYS I   361                                                      
REMARK 465     PHE I   362                                                      
REMARK 465     PRO I   363                                                      
REMARK 465     GLN I   364                                                      
REMARK 465     LEU I   365                                                      
REMARK 465     CYS I   366                                                      
REMARK 465     LYS I   367                                                      
REMARK 465     ALA I   368                                                      
REMARK 465     GLY I   369                                                      
REMARK 465     SER I   370                                                      
REMARK 465     PRO I   371                                                      
REMARK 465     ASP I   372                                                      
REMARK 465     ASP I   373                                                      
REMARK 465     VAL I   374                                                      
REMARK 465     SER I   375                                                      
REMARK 465     GLU I   376                                                      
REMARK 465     VAL I   377                                                      
REMARK 465     VAL I   378                                                      
REMARK 465     ASN I   379                                                      
REMARK 465     VAL I   380                                                      
REMARK 465     ALA I   381                                                      
REMARK 465     LEU I   382                                                      
REMARK 465     SER I   383                                                      
REMARK 465     GLY I   384                                                      
REMARK 465     VAL I   385                                                      
REMARK 465     ILE I   386                                                      
REMARK 465     ILE I   387                                                      
REMARK 465     ARG I   388                                                      
REMARK 465     PRO I   389                                                      
REMARK 465     VAL I   390                                                      
REMARK 465     THR I   391                                                      
REMARK 465     HIS I   392                                                      
REMARK 465     VAL I   393                                                      
REMARK 465     PRO I   394                                                      
REMARK 465     ALA I   395                                                      
REMARK 465     ALA I   396                                                      
REMARK 465     ILE I   397                                                      
REMARK 465     PRO I   398                                                      
REMARK 465     LEU I   399                                                      
REMARK 465     ARG I   400                                                      
REMARK 465     LEU I   401                                                      
REMARK 465     GLU I   402                                                      
REMARK 465     ASN I   403                                                      
REMARK 465     GLN I   404                                                      
REMARK 465     TYR I   405                                                      
REMARK 465     PHE I   406                                                      
REMARK 465     ALA I   407                                                      
REMARK 465     LEU I   408                                                      
REMARK 465     ASP I   409                                                      
REMARK 465     LEU I   410                                                      
REMARK 465     SER I   411                                                      
REMARK 465     THR I   412                                                      
REMARK 465     ASP I   413                                                      
REMARK 465     ALA I   414                                                      
REMARK 465     ALA I   415                                                      
REMARK 465     ARG I   416                                                      
REMARK 465     ALA I   417                                                      
REMARK 465     MET I   418                                                      
REMARK 465     LEU I   419                                                      
REMARK 465     ASP I   420                                                      
REMARK 465     ALA I   421                                                      
REMARK 465     GLY I   422                                                      
REMARK 465     ARG I   423                                                      
REMARK 465     CYS I   424                                                      
REMARK 465     THR I   425                                                      
REMARK 465     PHE I   426                                                      
REMARK 465     TYR I   427                                                      
REMARK 465     THR I   428                                                      
REMARK 465     PRO I   429                                                      
REMARK 465     ALA I   430                                                      
REMARK 465     SER I   431                                                      
REMARK 465     LEU I   432                                                      
REMARK 465     GLY I   433                                                      
REMARK 465     ASP I   434                                                      
REMARK 465     VAL I   435                                                      
REMARK 465     LYS I   436                                                      
REMARK 465     LEU I   437                                                      
REMARK 465     GLU I   438                                                      
REMARK 465     LEU I   439                                                      
REMARK 465     PHE I   440                                                      
REMARK 465     ALA I   441                                                      
REMARK 465     VAL I   442                                                      
REMARK 465     LEU I   443                                                      
REMARK 465     ARG I   444                                                      
REMARK 465     THR I   445                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ASN D 222    CG   OD1  ND2                                       
REMARK 470     ASN D 223    CG   OD1  ND2                                       
REMARK 470     ARG D 225    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG E 225    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASN F 159    CG   OD1  ND2                                       
REMARK 470     LEU H 334    CG   CD1  CD2                                       
REMARK 470     HIS H 335    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU H 347    CG   CD1  CD2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU G 314   C   -  N   -  CA  ANGL. DEV. =  15.8 DEGREES          
REMARK 500    LEU I  14   CA  -  CB  -  CG  ANGL. DEV. =  16.2 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG C 219     -140.20   -136.88                                   
REMARK 500    ILE C 220      -58.16    -27.88                                   
REMARK 500    PRO C 221      118.68    -33.97                                   
REMARK 500    THR C 227       -4.52     65.30                                   
REMARK 500    LYS C 237      -52.70   -120.67                                   
REMARK 500    MET C 242     -167.06   -164.10                                   
REMARK 500    PRO C 304     -166.18    -78.63                                   
REMARK 500    ASP C 305     -154.65   -137.33                                   
REMARK 500    ALA C 306       20.20    -63.66                                   
REMARK 500    ARG C 307      171.96    -55.96                                   
REMARK 500    LEU C 308     -159.70    -77.21                                   
REMARK 500    ARG C 311       55.18   -161.19                                   
REMARK 500    ALA C 314       49.62    -95.25                                   
REMARK 500    ALA D  39       47.37   -142.78                                   
REMARK 500    ALA D  46       68.34     60.52                                   
REMARK 500    SER D  57       51.65    -94.18                                   
REMARK 500    ASN D  81     -168.96   -115.36                                   
REMARK 500    SER D  95     -163.20    -78.96                                   
REMARK 500    ALA D 108      -60.47   -100.79                                   
REMARK 500    ASN D 112       85.10   -154.58                                   
REMARK 500    LEU D 113      143.34   -172.95                                   
REMARK 500    LEU D 135       35.05    -93.20                                   
REMARK 500    ASN D 149       77.40   -100.25                                   
REMARK 500    GLN D 157     -165.64    -79.16                                   
REMARK 500    VAL D 167      -62.69   -123.51                                   
REMARK 500    VAL D 231      -64.78   -121.75                                   
REMARK 500    LEU D 275       30.11    -92.57                                   
REMARK 500    VAL D 298      -64.89   -120.31                                   
REMARK 500    PRO E  41     -166.95    -70.89                                   
REMARK 500    SER E  89       48.97    -85.20                                   
REMARK 500    ARG E  94       32.35    -88.72                                   
REMARK 500    SER E  95     -169.60    -77.16                                   
REMARK 500    TRP E 125       68.07     61.68                                   
REMARK 500    ALA E 155       47.97    -90.12                                   
REMARK 500    ALA E 160     -156.74   -149.25                                   
REMARK 500    PRO E 257       44.15    -85.10                                   
REMARK 500    ARG E 259      149.35   -173.25                                   
REMARK 500    VAL E 298      -57.72   -121.28                                   
REMARK 500    ASP F  10       44.48    -87.16                                   
REMARK 500    PRO F  69        3.89    -64.49                                   
REMARK 500    ASN F  81      161.47    179.61                                   
REMARK 500    GLU F 134      -17.41   -140.10                                   
REMARK 500    ASN F 159       32.22   -141.39                                   
REMARK 500    ALA F 161     -163.57    -79.36                                   
REMARK 500    LEU F 163      -70.77    -28.99                                   
REMARK 500    THR F 164       17.55     46.60                                   
REMARK 500    PRO F 257       42.80    -85.88                                   
REMARK 500    ASP F 285       38.21    -99.55                                   
REMARK 500    VAL F 298      -63.49   -121.58                                   
REMARK 500    ARG G  35      -72.86    -76.48                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     109 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 ARG C  219     ILE C  220                 -128.09                    
REMARK 500 ILE C  220     PRO C  221                 -114.89                    
REMARK 500 LYS C  237     SER C  238                 -132.71                    
REMARK 500 ASP C  305     ALA C  306                 -139.32                    
REMARK 500 LEU C  308     SER C  309                 -135.24                    
REMARK 500 CYS C  310     ARG C  311                  127.03                    
REMARK 500 ARG E   78     ALA E   79                  146.13                    
REMARK 500 THR E  190     LEU E  191                 -138.73                    
REMARK 500 HIS F   40     PRO F   41                  146.26                    
REMARK 500 TRP F  162     LEU F  163                 -124.15                    
REMARK 500 SER F  196     LEU F  197                  147.99                    
REMARK 500 HIS G   40     PRO G   41                  131.68                    
REMARK 500 GLN G  133     GLU G  134                 -133.90                    
REMARK 500 ALA G  160     ALA G  161                  141.92                    
REMARK 500 MET G  256     PRO G  257                 -132.05                    
REMARK 500 LEU H   56     SER H   57                 -133.33                    
REMARK 500 LEU H  105     LEU H  106                 -143.57                    
REMARK 500 LYS H  361     PHE H  362                  145.98                    
REMARK 500 PRO I   41     TRP I   42                 -142.14                    
REMARK 500 LEU I   56     SER I   57                 -134.39                    
REMARK 500 GLN I  133     GLU I  134                 -147.50                    
REMARK 500 GLU I  139     GLN I  140                 -148.10                    
REMARK 500 PRO I  186     PRO I  187                 -134.09                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-0010   RELATED DB: EMDB                              
REMARK 900 THE BASEPLATE COMPLEX FROM THE TYPE VI SECRETION SYSTEM              
DBREF  6GJ3 C  100   366  UNP    H4UNW2   H4UNW2_ECOLX     1    267             
DBREF  6GJ3 D    1   445  UNP    H4UNX9   H4UNX9_ECOLX     1    445             
DBREF  6GJ3 E    1   445  UNP    H4UNX9   H4UNX9_ECOLX     1    445             
DBREF  6GJ3 F    1   445  UNP    H4UNX9   H4UNX9_ECOLX     1    445             
DBREF  6GJ3 G    1   445  UNP    H4UNX9   H4UNX9_ECOLX     1    445             
DBREF  6GJ3 H    1   445  UNP    H4UNX9   H4UNX9_ECOLX     1    445             
DBREF  6GJ3 I    1   445  UNP    H4UNX9   H4UNX9_ECOLX     1    445             
SEQADV 6GJ3 THR C  332  UNP  H4UNW2    ALA   233 CONFLICT                       
SEQADV 6GJ3 LEU D  202  UNP  H4UNX9    ALA   202 CONFLICT                       
SEQADV 6GJ3 LEU E  202  UNP  H4UNX9    ALA   202 CONFLICT                       
SEQADV 6GJ3 LEU F  202  UNP  H4UNX9    ALA   202 CONFLICT                       
SEQADV 6GJ3 LEU G  202  UNP  H4UNX9    ALA   202 CONFLICT                       
SEQADV 6GJ3 LEU H  202  UNP  H4UNX9    ALA   202 CONFLICT                       
SEQADV 6GJ3 LEU I  202  UNP  H4UNX9    ALA   202 CONFLICT                       
SEQRES   1 C  267  MET PRO ALA HIS TYR ILE SER ASP ILE ALA GLN GLN ARG          
SEQRES   2 C  267  GLU GLY HIS GLU ALA ALA ALA ASP PHE LEU ASP ILE PHE          
SEQRES   3 C  267  SER HIS ARG LEU ILE THR GLN TYR TYR ARG ILE TRP ARG          
SEQRES   4 C  267  LYS TYR SER TYR PRO ALA THR PHE GLU ALA GLY GLY GLN          
SEQRES   5 C  267  ASP LYS THR SER GLN TYR LEU LEU GLY LEU ALA ARG LEU          
SEQRES   6 C  267  GLY ILE PRO GLY CYS ALA GLN ASN ILE ALA THR PRO VAL          
SEQRES   7 C  267  SER ARG PHE LEU ALA LEU LEU PRO LEU MET LEU LEU PRO          
SEQRES   8 C  267  GLY ARG THR ALA GLU GLY LEU THR SER LEU VAL THR LEU          
SEQRES   9 C  267  LEU ALA PRO GLY THR GLN ALA ARG VAL TRP HIS HIS ASP          
SEQRES  10 C  267  ARG ARG ARG ILE PRO LEU LYS THR PRO LEU THR MET ARG          
SEQRES  11 C  267  VAL HIS HIS PRO VAL SER LEU LYS SER ARG PRO VAL MET          
SEQRES  12 C  267  GLY ASP HIS ALA THR ASP VAL ASN GLY GLN VAL LEU LEU          
SEQRES  13 C  267  GLN LEU SER THR GLN THR GLY SER GLU VAL GLN GLY TRP          
SEQRES  14 C  267  LEU PRO GLY GLY HIS LEU TYR SER ASP LEU LEU ALA LEU          
SEQRES  15 C  267  LEU HIS VAL TYR LEU GLY SER ARG LEU ASP VAL ARG LEU          
SEQRES  16 C  267  GLN LEU CYS VAL GLU ARG SER LEU LEU PRO ASP ALA ARG          
SEQRES  17 C  267  LEU SER CYS ARG PRO ALA ALA GLY SER PRO GLN LEU GLY          
SEQRES  18 C  267  ARG THR ALA VAL MET ARG THR GLN ALA LYS ILE THR THR          
SEQRES  19 C  267  SER ALA ALA ARG VAL MET THR ILE SER LEU GLY ARG TYR          
SEQRES  20 C  267  GLN ARG VAL GLN GLU HIS TYR GLN ARG LYS GLU THR GLN          
SEQRES  21 C  267  GLU ASN GLY ASP TYR ARG TRP                                  
SEQRES   1 D  445  MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE          
SEQRES   2 D  445  LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP          
SEQRES   3 D  445  VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA          
SEQRES   4 D  445  HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER          
SEQRES   5 D  445  LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE          
SEQRES   6 D  445  VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG          
SEQRES   7 D  445  ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL          
SEQRES   8 D  445  SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO          
SEQRES   9 D  445  LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER          
SEQRES  10 D  445  GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL          
SEQRES  11 D  445  ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL          
SEQRES  12 D  445  ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS          
SEQRES  13 D  445  GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG          
SEQRES  14 D  445  LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO          
SEQRES  15 D  445  ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO          
SEQRES  16 D  445  SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU          
SEQRES  17 D  445  GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU          
SEQRES  18 D  445  ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL          
SEQRES  19 D  445  SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU          
SEQRES  20 D  445  PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS          
SEQRES  21 D  445  PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY          
SEQRES  22 D  445  SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA          
SEQRES  23 D  445  VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE          
SEQRES  24 D  445  PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA          
SEQRES  25 D  445  SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN          
SEQRES  26 D  445  LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG          
SEQRES  27 D  445  LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER          
SEQRES  28 D  445  SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN          
SEQRES  29 D  445  LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL          
SEQRES  30 D  445  VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL          
SEQRES  31 D  445  THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN          
SEQRES  32 D  445  GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG          
SEQRES  33 D  445  ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO          
SEQRES  34 D  445  ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL          
SEQRES  35 D  445  LEU ARG THR                                                  
SEQRES   1 E  445  MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE          
SEQRES   2 E  445  LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP          
SEQRES   3 E  445  VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA          
SEQRES   4 E  445  HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER          
SEQRES   5 E  445  LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE          
SEQRES   6 E  445  VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG          
SEQRES   7 E  445  ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL          
SEQRES   8 E  445  SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO          
SEQRES   9 E  445  LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER          
SEQRES  10 E  445  GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL          
SEQRES  11 E  445  ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL          
SEQRES  12 E  445  ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS          
SEQRES  13 E  445  GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG          
SEQRES  14 E  445  LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO          
SEQRES  15 E  445  ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO          
SEQRES  16 E  445  SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU          
SEQRES  17 E  445  GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU          
SEQRES  18 E  445  ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL          
SEQRES  19 E  445  SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU          
SEQRES  20 E  445  PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS          
SEQRES  21 E  445  PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY          
SEQRES  22 E  445  SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA          
SEQRES  23 E  445  VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE          
SEQRES  24 E  445  PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA          
SEQRES  25 E  445  SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN          
SEQRES  26 E  445  LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG          
SEQRES  27 E  445  LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER          
SEQRES  28 E  445  SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN          
SEQRES  29 E  445  LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL          
SEQRES  30 E  445  VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL          
SEQRES  31 E  445  THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN          
SEQRES  32 E  445  GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG          
SEQRES  33 E  445  ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO          
SEQRES  34 E  445  ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL          
SEQRES  35 E  445  LEU ARG THR                                                  
SEQRES   1 F  445  MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE          
SEQRES   2 F  445  LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP          
SEQRES   3 F  445  VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA          
SEQRES   4 F  445  HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER          
SEQRES   5 F  445  LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE          
SEQRES   6 F  445  VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG          
SEQRES   7 F  445  ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL          
SEQRES   8 F  445  SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO          
SEQRES   9 F  445  LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER          
SEQRES  10 F  445  GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL          
SEQRES  11 F  445  ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL          
SEQRES  12 F  445  ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS          
SEQRES  13 F  445  GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG          
SEQRES  14 F  445  LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO          
SEQRES  15 F  445  ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO          
SEQRES  16 F  445  SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU          
SEQRES  17 F  445  GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU          
SEQRES  18 F  445  ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL          
SEQRES  19 F  445  SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU          
SEQRES  20 F  445  PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS          
SEQRES  21 F  445  PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY          
SEQRES  22 F  445  SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA          
SEQRES  23 F  445  VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE          
SEQRES  24 F  445  PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA          
SEQRES  25 F  445  SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN          
SEQRES  26 F  445  LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG          
SEQRES  27 F  445  LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER          
SEQRES  28 F  445  SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN          
SEQRES  29 F  445  LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL          
SEQRES  30 F  445  VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL          
SEQRES  31 F  445  THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN          
SEQRES  32 F  445  GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG          
SEQRES  33 F  445  ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO          
SEQRES  34 F  445  ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL          
SEQRES  35 F  445  LEU ARG THR                                                  
SEQRES   1 G  445  MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE          
SEQRES   2 G  445  LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP          
SEQRES   3 G  445  VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA          
SEQRES   4 G  445  HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER          
SEQRES   5 G  445  LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE          
SEQRES   6 G  445  VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG          
SEQRES   7 G  445  ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL          
SEQRES   8 G  445  SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO          
SEQRES   9 G  445  LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER          
SEQRES  10 G  445  GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL          
SEQRES  11 G  445  ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL          
SEQRES  12 G  445  ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS          
SEQRES  13 G  445  GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG          
SEQRES  14 G  445  LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO          
SEQRES  15 G  445  ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO          
SEQRES  16 G  445  SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU          
SEQRES  17 G  445  GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU          
SEQRES  18 G  445  ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL          
SEQRES  19 G  445  SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU          
SEQRES  20 G  445  PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS          
SEQRES  21 G  445  PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY          
SEQRES  22 G  445  SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA          
SEQRES  23 G  445  VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE          
SEQRES  24 G  445  PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA          
SEQRES  25 G  445  SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN          
SEQRES  26 G  445  LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG          
SEQRES  27 G  445  LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER          
SEQRES  28 G  445  SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN          
SEQRES  29 G  445  LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL          
SEQRES  30 G  445  VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL          
SEQRES  31 G  445  THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN          
SEQRES  32 G  445  GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG          
SEQRES  33 G  445  ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO          
SEQRES  34 G  445  ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL          
SEQRES  35 G  445  LEU ARG THR                                                  
SEQRES   1 H  445  MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE          
SEQRES   2 H  445  LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP          
SEQRES   3 H  445  VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA          
SEQRES   4 H  445  HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER          
SEQRES   5 H  445  LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE          
SEQRES   6 H  445  VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG          
SEQRES   7 H  445  ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL          
SEQRES   8 H  445  SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO          
SEQRES   9 H  445  LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER          
SEQRES  10 H  445  GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL          
SEQRES  11 H  445  ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL          
SEQRES  12 H  445  ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS          
SEQRES  13 H  445  GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG          
SEQRES  14 H  445  LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO          
SEQRES  15 H  445  ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO          
SEQRES  16 H  445  SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU          
SEQRES  17 H  445  GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU          
SEQRES  18 H  445  ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL          
SEQRES  19 H  445  SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU          
SEQRES  20 H  445  PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS          
SEQRES  21 H  445  PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY          
SEQRES  22 H  445  SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA          
SEQRES  23 H  445  VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE          
SEQRES  24 H  445  PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA          
SEQRES  25 H  445  SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN          
SEQRES  26 H  445  LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG          
SEQRES  27 H  445  LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER          
SEQRES  28 H  445  SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN          
SEQRES  29 H  445  LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL          
SEQRES  30 H  445  VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL          
SEQRES  31 H  445  THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN          
SEQRES  32 H  445  GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG          
SEQRES  33 H  445  ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO          
SEQRES  34 H  445  ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL          
SEQRES  35 H  445  LEU ARG THR                                                  
SEQRES   1 I  445  MET LYS ILE TYR ARG PRO LEU TRP GLU ASP GLY ALA PHE          
SEQRES   2 I  445  LEU MET PRO GLN GLN PHE GLN GLN GLN ALA ALA TRP ASP          
SEQRES   3 I  445  VAL HIS LEU ALA ASP SER VAL ALA ARG MET GLY LEU ALA          
SEQRES   4 I  445  HIS PRO TRP GLY VAL VAL ALA ALA GLU PHE ASP ASP SER          
SEQRES   5 I  445  LEU LEU PRO LEU SER ARG LEU ASN ALA THR ARG LEU ILE          
SEQRES   6 I  445  VAL ARG PHE PRO ASP GLY THR LEU ILE ASP THR GLU ARG          
SEQRES   7 I  445  ALA ASP ASN LEU PRO PRO VAL CYS ASP LEU SER THR VAL          
SEQRES   8 I  445  SER ASP ARG SER LEU VAL ASP ILE VAL LEU ALA LEU PRO          
SEQRES   9 I  445  LEU LEU ASN ALA ASN GLY GLY ASN LEU ASP ASN GLY SER          
SEQRES  10 I  445  GLU SER GLU ARG PRO ARG ARG TRP LYS SER GLU ARG VAL          
SEQRES  11 I  445  ASN VAL GLN GLU LEU ALA GLY HIS GLU GLN SER GLU VAL          
SEQRES  12 I  445  ALA VAL LEU ARG HIS ASN LEU THR LEU ARG MET ALA HIS          
SEQRES  13 I  445  GLN GLU ASN ALA ALA TRP LEU THR CYS PRO VAL THR ARG          
SEQRES  14 I  445  LEU VAL ARG ASP ALA GLN GLY GLN TRP CYS ARG ASP PRO          
SEQRES  15 I  445  ARG PHE ILE PRO PRO LEU LEU THR LEU SER ALA SER PRO          
SEQRES  16 I  445  SER LEU MET THR GLU LEU LEU GLU LEU LEU HIS HIS LEU          
SEQRES  17 I  445  GLN ALA ARG ARG GLN ARG LEU MET SER MET ARG ARG GLU          
SEQRES  18 I  445  ASN ASN ALA ARG LEU ALA ASP PHE ALA VAL ALA ASP VAL          
SEQRES  19 I  445  SER LEU PHE TRP LEU LEU ASN ALA LEU ASN SER ALA GLU          
SEQRES  20 I  445  PRO VAL LEU LYS GLU LEU LEU ASP MET PRO TYR ARG HIS          
SEQRES  21 I  445  PRO GLU LEU LEU TYR ARG GLU LEU ALA ARG LEU ALA GLY          
SEQRES  22 I  445  SER LEU LEU THR PHE SER LEU GLU HIS ASN VAL ASP ALA          
SEQRES  23 I  445  VAL PRO ALA TYR HIS HIS GLU THR PRO GLU ASN VAL PHE          
SEQRES  24 I  445  PRO PRO LEU LEU SER LEU LEU ASN ARG LEU LEU GLU ALA          
SEQRES  25 I  445  SER LEU PRO SER ARG VAL VAL PHE ILE GLU LEU LYS GLN          
SEQRES  26 I  445  LYS GLY VAL MET TRP GLU GLY ALA LEU HIS ASP ALA ARG          
SEQRES  27 I  445  LEU ARG GLU GLY ALA ASP PHE TRP LEU SER VAL ARG SER          
SEQRES  28 I  445  SER MET PRO GLY HIS GLU LEU GLN THR LYS PHE PRO GLN          
SEQRES  29 I  445  LEU CYS LYS ALA GLY SER PRO ASP ASP VAL SER GLU VAL          
SEQRES  30 I  445  VAL ASN VAL ALA LEU SER GLY VAL ILE ILE ARG PRO VAL          
SEQRES  31 I  445  THR HIS VAL PRO ALA ALA ILE PRO LEU ARG LEU GLU ASN          
SEQRES  32 I  445  GLN TYR PHE ALA LEU ASP LEU SER THR ASP ALA ALA ARG          
SEQRES  33 I  445  ALA MET LEU ASP ALA GLY ARG CYS THR PHE TYR THR PRO          
SEQRES  34 I  445  ALA SER LEU GLY ASP VAL LYS LEU GLU LEU PHE ALA VAL          
SEQRES  35 I  445  LEU ARG THR                                                  
HELIX    1 AA1 MET D   15  ARG D   35  1                                  21    
HELIX    2 AA2 THR D  190  ALA D  193  5                                   4    
HELIX    3 AA3 SER D  194  MET D  218  1                                  25    
HELIX    4 AA4 SER D  235  SER D  245  1                                  11    
HELIX    5 AA5 ALA D  246  MET D  256  1                                  11    
HELIX    6 AA6 HIS D  260  LEU D  275  1                                  16    
HELIX    7 AA7 THR D  294  ASN D  297  5                                   4    
HELIX    8 AA8 VAL D  298  LEU D  310  1                                  13    
HELIX    9 AA9 MET E   15  VAL E   33  1                                  19    
HELIX   10 AB1 THR E  190  ALA E  193  5                                   4    
HELIX   11 AB2 SER E  194  MET E  218  1                                  25    
HELIX   12 AB3 LEU E  236  ALA E  242  1                                   7    
HELIX   13 AB4 ALA E  246  LYS E  251  1                                   6    
HELIX   14 AB5 PRO E  261  THR E  277  1                                  17    
HELIX   15 AB6 THR E  294  ASN E  297  5                                   4    
HELIX   16 AB7 VAL E  298  LEU E  310  1                                  13    
HELIX   17 AB8 PRO F   16  ARG F   35  1                                  20    
HELIX   18 AB9 LEU F   53  SER F   57  5                                   5    
HELIX   19 AC1 SER F  194  ASN F  222  1                                  29    
HELIX   20 AC2 ALA F  230  ASN F  244  1                                  15    
HELIX   21 AC3 ALA F  246  LYS F  251  1                                   6    
HELIX   22 AC4 PRO F  261  LEU F  275  1                                  15    
HELIX   23 AC5 THR F  294  ASN F  297  5                                   4    
HELIX   24 AC6 VAL F  298  LEU F  310  1                                  13    
HELIX   25 AC7 PRO G   16  MET G   36  1                                  21    
HELIX   26 AC8 PRO G  195  MET G  218  1                                  24    
HELIX   27 AC9 MET G  218  ASN G  223  1                                   6    
HELIX   28 AD1 ALA G  230  SER G  245  1                                  16    
HELIX   29 AD2 ALA G  246  LEU G  253  1                                   8    
HELIX   30 AD3 HIS G  260  LEU G  276  1                                  17    
HELIX   31 AD4 THR G  294  ASN G  297  5                                   4    
HELIX   32 AD5 VAL G  298  LEU G  310  1                                  13    
HELIX   33 AD6 PRO G  354  CYS G  366  1                                  13    
HELIX   34 AD7 SER G  411  ASP G  420  1                                  10    
HELIX   35 AD8 MET H   15  ARG H   35  1                                  21    
HELIX   36 AD9 SER H  194  ASN H  223  1                                  30    
HELIX   37 AE1 VAL H  231  LEU H  254  1                                  24    
HELIX   38 AE2 HIS H  260  LEU H  275  1                                  16    
HELIX   39 AE3 ASN H  283  VAL H  287  5                                   5    
HELIX   40 AE4 VAL H  298  LEU H  310  1                                  13    
HELIX   41 AE5 ASP H  336  GLU H  341  5                                   6    
HELIX   42 AE6 THR H  412  GLY H  422  1                                  11    
HELIX   43 AE7 MET I   15  ALA I   24  1                                  10    
HELIX   44 AE8 TRP I   25  ARG I   35  1                                  11    
HELIX   45 AE9 ASP I   51  LEU I   56  5                                   6    
HELIX   46 AF1 SER I   89  SER I   92  5                                   4    
HELIX   47 AF2 SER I  194  ARG I  220  1                                  27    
HELIX   48 AF3 ALA I  224  ASP I  228  5                                   5    
HELIX   49 AF4 ALA I  230  SER I  245  1                                  16    
HELIX   50 AF5 ALA I  246  LEU I  254  1                                   9    
HELIX   51 AF6 HIS I  260  LEU I  276  1                                  17    
HELIX   52 AF7 ASN I  283  VAL I  287  5                                   5    
HELIX   53 AF8 VAL I  298  LEU I  310  1                                  13    
SHEET    1 AA1 3 VAL D  44  PHE D  49  0                                        
SHEET    2 AA1 3 ALA D  61  ARG D  67 -1  O  ILE D  65   N  VAL D  45           
SHEET    3 AA1 3 LEU D  73  ASP D  75 -1  O  ILE D  74   N  VAL D  66           
SHEET    1 AA2 4 THR D 151  ARG D 153  0                                        
SHEET    2 AA2 4 VAL D  97  ALA D 102 -1  N  VAL D 100   O  ARG D 153           
SHEET    3 AA2 4 CYS D 165  ARG D 172 -1  O  LEU D 170   N  VAL D  97           
SHEET    4 AA2 4 TRP D 178  ARG D 180 -1  O  CYS D 179   N  VAL D 171           
SHEET    1 AA3 2 ARG D 124  ASN D 131  0                                        
SHEET    2 AA3 2 GLU D 142  HIS D 148 -1  O  VAL D 145   N  SER D 127           
SHEET    1 AA4 3 VAL E  44  PHE E  49  0                                        
SHEET    2 AA4 3 ALA E  61  ARG E  67 -1  O  ILE E  65   N  ALA E  46           
SHEET    3 AA4 3 LEU E  73  ASP E  75 -1  O  ILE E  74   N  VAL E  66           
SHEET    1 AA5 3 THR E 151  MET E 154  0                                        
SHEET    2 AA5 3 VAL E  97  PRO E 104 -1  N  VAL E 100   O  ARG E 153           
SHEET    3 AA5 3 TRP E 162  LEU E 163 -1  O  LEU E 163   N  LEU E 103           
SHEET    1 AA6 4 THR E 151  MET E 154  0                                        
SHEET    2 AA6 4 VAL E  97  PRO E 104 -1  N  VAL E 100   O  ARG E 153           
SHEET    3 AA6 4 PRO E 166  ASP E 173 -1  O  VAL E 167   N  ILE E  99           
SHEET    4 AA6 4 GLN E 177  CYS E 179 -1  O  GLN E 177   N  ASP E 173           
SHEET    1 AA7 2 LYS E 126  GLN E 133  0                                        
SHEET    2 AA7 2 GLN E 140  ARG E 147 -1  O  SER E 141   N  VAL E 132           
SHEET    1 AA8 3 VAL F  44  PHE F  49  0                                        
SHEET    2 AA8 3 ALA F  61  VAL F  66 -1  O  ILE F  65   N  VAL F  45           
SHEET    3 AA8 3 ILE F  74  ASP F  75 -1  O  ILE F  74   N  VAL F  66           
SHEET    1 AA9 2 GLU F 128  VAL F 132  0                                        
SHEET    2 AA9 2 SER F 141  VAL F 145 -1  O  VAL F 143   N  VAL F 130           
SHEET    1 AB1 2 LEU F 170  ASP F 173  0                                        
SHEET    2 AB1 2 GLN F 177  ARG F 180 -1  O  CYS F 179   N  VAL F 171           
SHEET    1 AB2 3 VAL G  44  ASP G  50  0                                        
SHEET    2 AB2 3 ARG G  58  ARG G  67 -1  O  ILE G  65   N  VAL G  45           
SHEET    3 AB2 3 LEU G  73  ASP G  75 -1  O  ILE G  74   N  VAL G  66           
SHEET    1 AB3 3 VAL G  44  ASP G  50  0                                        
SHEET    2 AB3 3 ARG G  58  ARG G  67 -1  O  ILE G  65   N  VAL G  45           
SHEET    3 AB3 3 CYS G  86  ASP G  87 -1  O  CYS G  86   N  LEU G  59           
SHEET    1 AB4 3 THR G 151  MET G 154  0                                        
SHEET    2 AB4 3 ASP G  98  ALA G 102 -1  N  VAL G 100   O  ARG G 153           
SHEET    3 AB4 3 CYS G 165  ARG G 169 -1  O  VAL G 167   N  ILE G  99           
SHEET    1 AB5 2 TRP G 125  ASN G 131  0                                        
SHEET    2 AB5 2 GLU G 142  ARG G 147 -1  O  VAL G 143   N  VAL G 130           
SHEET    1 AB6 4 VAL G 318  ILE G 321  0                                        
SHEET    2 AB6 4 VAL G 435  VAL G 442 -1  O  LEU G 439   N  ILE G 321           
SHEET    3 AB6 4 PHE G 345  SER G 351 -1  N  ARG G 350   O  LYS G 436           
SHEET    4 AB6 4 TYR G 405  ALA G 407 -1  O  PHE G 406   N  LEU G 347           
SHEET    1 AB7 4 GLN G 325  LYS G 326  0                                        
SHEET    2 AB7 4 MET G 329  ALA G 333 -1  O  MET G 329   N  LYS G 326           
SHEET    3 AB7 4 ARG G 423  TYR G 427 -1  O  PHE G 426   N  TRP G 330           
SHEET    4 AB7 4 LYS G 367  GLY G 369 -1  N  LYS G 367   O  TYR G 427           
SHEET    1 AB8 3 VAL H  44  PHE H  49  0                                        
SHEET    2 AB8 3 ALA H  61  ARG H  67 -1  O  ILE H  65   N  VAL H  45           
SHEET    3 AB8 3 LEU H  73  ILE H  74 -1  O  ILE H  74   N  VAL H  66           
SHEET    1 AB9 4 THR H 151  MET H 154  0                                        
SHEET    2 AB9 4 LEU H  96  ALA H 102 -1  N  VAL H 100   O  ARG H 153           
SHEET    3 AB9 4 CYS H 165  ARG H 172 -1  O  LEU H 170   N  VAL H  97           
SHEET    4 AB9 4 TRP H 178  ARG H 180 -1  O  CYS H 179   N  VAL H 171           
SHEET    1 AC1 2 TRP H 125  VAL H 132  0                                        
SHEET    2 AC1 2 SER H 141  HIS H 148 -1  O  ARG H 147   N  LYS H 126           
SHEET    1 AC2 2 ILE H 387  VAL H 390  0                                        
SHEET    2 AC2 2 TYR H 405  LEU H 408 -1  O  TYR H 405   N  VAL H 390           
SHEET    1 AC3 3 VAL I  44  PHE I  49  0                                        
SHEET    2 AC3 3 ALA I  61  PHE I  68 -1  O  ILE I  65   N  ALA I  46           
SHEET    3 AC3 3 THR I  72  LEU I  73 -1  O  THR I  72   N  PHE I  68           
SHEET    1 AC4 2 ARG I  58  LEU I  59  0                                        
SHEET    2 AC4 2 CYS I  86  ASP I  87 -1  O  CYS I  86   N  LEU I  59           
SHEET    1 AC5 4 THR I 151  ARG I 153  0                                        
SHEET    2 AC5 4 VAL I  97  PRO I 104 -1  N  VAL I 100   O  ARG I 153           
SHEET    3 AC5 4 TRP I 162  ARG I 172 -1  O  VAL I 167   N  ILE I  99           
SHEET    4 AC5 4 TRP I 178  ARG I 180 -1  O  CYS I 179   N  VAL I 171           
SHEET    1 AC6 2 LYS I 126  SER I 127  0                                        
SHEET    2 AC6 2 LEU I 146  ARG I 147 -1  O  ARG I 147   N  LYS I 126           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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