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Database: PDB
Entry: 6GVL
LinkDB: 6GVL
Original site: 6GVL 
HEADER    STRUCTURAL PROTEIN                      21-JUN-18   6GVL              
TITLE     SECOND PAIR OF FIBRONECTIN TYPE III DOMAINS OF INTEGRIN BETA4 BOUND TO
TITLE    2 THE BULLOUS PEMPHIGOID ANTIGEN BP230 (BPAG1E)                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: INTEGRIN BETA-4;                                           
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: GP150;                                                      
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: DYSTONIN;                                                  
COMPND   8 CHAIN: B;                                                            
COMPND   9 SYNONYM: 230 KDA BULLOUS PEMPHIGOID ANTIGEN,230/240 KDA BULLOUS      
COMPND  10 PEMPHIGOID ANTIGEN,BULLOUS PEMPHIGOID ANTIGEN 1,BULLOUS PEMPHIGOID   
COMPND  11 ANTIGEN,DYSTONIA MUSCULORUM PROTEIN,HEMIDESMOSOMAL PLAQUE PROTEIN;   
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ITGB4;                                                         
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: MODIFIED PET15B;                          
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 SYNTHETIC: YES;                                                      
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606                                                 
KEYWDS    CYTOSKELETON, PLAKIN, HEMIDESMOSOME, STRUCTURAL PROTEIN               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.A.MANSO,M.GOMEZ-HERNANDEZ,N.ALONSO-GARCIA,J.M.DE PEREDA             
REVDAT   3   19-JUN-19 6GVL    1       JRNL                                     
REVDAT   2   01-MAY-19 6GVL    1       JRNL                                     
REVDAT   1   20-MAR-19 6GVL    0                                                
JRNL        AUTH   J.A.MANSO,M.GOMEZ-HERNANDEZ,A.CARABIAS,N.ALONSO-GARCIA,      
JRNL        AUTH 2 I.GARCIA-RUBIO,M.KREFT,A.SONNENBERG,J.M.DE PEREDA            
JRNL        TITL   INTEGRIN ALPHA 6 BETA 4 RECOGNITION OF A LINEAR MOTIF OF     
JRNL        TITL 2 BULLOUS PEMPHIGOID ANTIGEN BP230 CONTROLS ITS RECRUITMENT TO 
JRNL        TITL 3 HEMIDESMOSOMES.                                              
JRNL        REF    STRUCTURE                     V.  27   952 2019              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   31006587                                                     
JRNL        DOI    10.1016/J.STR.2019.03.016                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.05 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (DEV_2499: ???)                               
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.05                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 47.85                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 14722                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.225                           
REMARK   3   R VALUE            (WORKING SET) : 0.224                           
REMARK   3   FREE R VALUE                     : 0.236                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.780                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 703                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 47.8630 -  3.5052    1.00     2852   146  0.1918 0.2026        
REMARK   3     2  3.5052 -  2.7822    1.00     2845   118  0.2220 0.2368        
REMARK   3     3  2.7822 -  2.4306    1.00     2750   174  0.2805 0.2944        
REMARK   3     4  2.4306 -  2.2083    0.99     2811   119  0.3034 0.3426        
REMARK   3     5  2.2083 -  2.0501    0.99     2761   146  0.3406 0.3394        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.320            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 31.030           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 45.80                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.002           1716                                  
REMARK   3   ANGLE     :  0.496           2345                                  
REMARK   3   CHIRALITY :  0.045            266                                  
REMARK   3   PLANARITY :  0.003            313                                  
REMARK   3   DIHEDRAL  : 14.525           1028                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 1457:1551)                          
REMARK   3    ORIGIN FOR THE GROUP (A):   6.3558   1.8123  33.1052              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5092 T22:   0.5081                                     
REMARK   3      T33:   0.5259 T12:   0.0206                                     
REMARK   3      T13:  -0.0408 T23:   0.0091                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4001 L22:   1.0134                                     
REMARK   3      L33:   1.9434 L12:  -0.4162                                     
REMARK   3      L13:   0.0046 L23:   0.0245                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0224 S12:   0.0143 S13:  -0.3249                       
REMARK   3      S21:  -0.1359 S22:   0.0323 S23:   0.1358                       
REMARK   3      S31:   0.5017 S32:  -0.0739 S33:  -0.0000                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 1561:1666)                          
REMARK   3    ORIGIN FOR THE GROUP (A):   5.2438  21.5485  18.2930              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4097 T22:   0.3849                                     
REMARK   3      T33:   0.4342 T12:  -0.0040                                     
REMARK   3      T13:   0.0095 T23:   0.0011                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3440 L22:   0.5314                                     
REMARK   3      L33:   2.6295 L12:   0.1522                                     
REMARK   3      L13:   0.6258 L23:  -0.1337                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0466 S12:  -0.0379 S13:  -0.0116                       
REMARK   3      S21:  -0.2156 S22:  -0.0033 S23:   0.0233                       
REMARK   3      S31:   0.2408 S32:  -0.0478 S33:   0.0000                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: (CHAIN B AND RESID 27:35)                              
REMARK   3    ORIGIN FOR THE GROUP (A):  10.8428  30.7832  28.1819              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6254 T22:   0.7130                                     
REMARK   3      T33:   0.6937 T12:  -0.0436                                     
REMARK   3      T13:  -0.0533 T23:   0.0626                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1887 L22:   0.0836                                     
REMARK   3      L33:   0.1035 L12:  -0.1251                                     
REMARK   3      L13:  -0.0128 L23:  -0.0011                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0424 S12:  -0.8274 S13:   0.6594                       
REMARK   3      S21:   0.3310 S22:   0.1520 S23:   0.0215                       
REMARK   3      S31:  -0.8303 S32:   0.0402 S33:   0.0005                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: (CHAIN B AND RESID 36:49)                              
REMARK   3    ORIGIN FOR THE GROUP (A):   9.8185  19.0467  24.2516              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6248 T22:   0.6095                                     
REMARK   3      T33:   0.4726 T12:  -0.0208                                     
REMARK   3      T13:   0.0299 T23:  -0.0011                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0252 L22:   0.1221                                     
REMARK   3      L33:   0.3056 L12:  -0.0155                                     
REMARK   3      L13:  -0.0670 L23:   0.1645                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0320 S12:   0.0935 S13:   0.0695                       
REMARK   3      S21:   0.0491 S22:  -0.0072 S23:  -0.4522                       
REMARK   3      S31:   0.1956 S32:   0.4777 S33:   0.0010                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6GVL COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 21-JUN-18.                  
REMARK 100 THE DEPOSITION ID IS D_1200010593.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 30-JUL-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 4.2                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : DIAMOND                            
REMARK 200  BEAMLINE                       : I03                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97625                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 6M                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS MAY 1, 2016                    
REMARK 200  DATA SCALING SOFTWARE          : XSCALE MAY 1, 2016                 
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 14766                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.050                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.5                               
REMARK 200  DATA REDUNDANCY                : 6.700                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 11.7000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.05                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.10                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.90                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.280                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: FOURIER SYNTHESIS            
REMARK 200 SOFTWARE USED: PHENIX DEV_2499                                       
REMARK 200 STARTING MODEL: D_1200010415                                         
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 46.00                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.23                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 PHOSPHATE-CITRATE PH 4.2, 20% PEG    
REMARK 280  8000, 0.2 M NACL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K   
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       52.26000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       30.36500            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       52.26000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       30.36500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2850 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 11230 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -19.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A  1453                                                      
REMARK 465     SER A  1454                                                      
REMARK 465     HIS A  1455                                                      
REMARK 465     MET A  1456                                                      
REMARK 465     GLU A  1484                                                      
REMARK 465     ARG A  1485                                                      
REMARK 465     VAL A  1552                                                      
REMARK 465     HIS A  1553                                                      
REMARK 465     PRO A  1554                                                      
REMARK 465     GLN A  1555                                                      
REMARK 465     SER A  1556                                                      
REMARK 465     PRO A  1557                                                      
REMARK 465     LEU A  1558                                                      
REMARK 465     CYS A  1559                                                      
REMARK 465     PRO A  1560                                                      
REMARK 465     ASP B    26                                                      
REMARK 465     GLU B    50                                                      
REMARK 465     SER B    51                                                      
REMARK 465     PHE B    52                                                      
REMARK 465     ASP B    53                                                      
REMARK 465     GLY B    54                                                      
REMARK 465     HIS B    55                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A1482    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     CYS A1483    SG                                                  
REMARK 470     PRO A1486    N    CB   CG   CD                                   
REMARK 470     GLN A1535    CG   CD   OE1  NE2                                  
REMARK 470     GLN A1551    CG   CD   OE1  NE2                                  
REMARK 470     GLN A1612    CG   CD   OE1  NE2                                  
REMARK 470     GLU A1652    CG   CD   OE1  OE2                                  
REMARK 470     SER B  49    OG                                                  
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 6GVK   RELATED DB: PDB                                   
REMARK 900 SAME COMPLEX OBTAINED WITH A HIGH AFFINITY BETA4 MUTANT              
DBREF  6GVL A 1457  1666  UNP    P16144   ITB4_HUMAN    1527   1736             
DBREF  6GVL B   26    55  UNP    Q03001   DYST_HUMAN      26     55             
SEQADV 6GVL GLY A 1453  UNP  P16144              EXPRESSION TAG                 
SEQADV 6GVL SER A 1454  UNP  P16144              EXPRESSION TAG                 
SEQADV 6GVL HIS A 1455  UNP  P16144              EXPRESSION TAG                 
SEQADV 6GVL MET A 1456  UNP  P16144              EXPRESSION TAG                 
SEQRES   1 A  214  GLY SER HIS MET VAL PRO ASP THR PRO THR ARG LEU VAL          
SEQRES   2 A  214  PHE SER ALA LEU GLY PRO THR SER LEU ARG VAL SER TRP          
SEQRES   3 A  214  GLN GLU PRO ARG CYS GLU ARG PRO LEU GLN GLY TYR SER          
SEQRES   4 A  214  VAL GLU TYR GLN LEU LEU ASN GLY GLY GLU LEU HIS ARG          
SEQRES   5 A  214  LEU ASN ILE PRO ASN PRO ALA GLN THR SER VAL VAL VAL          
SEQRES   6 A  214  GLU ASP LEU LEU PRO ASN HIS SER TYR VAL PHE ARG VAL          
SEQRES   7 A  214  ARG ALA GLN SER GLN GLU GLY TRP GLY ARG GLU ARG GLU          
SEQRES   8 A  214  GLY VAL ILE THR ILE GLU SER GLN VAL HIS PRO GLN SER          
SEQRES   9 A  214  PRO LEU CYS PRO LEU PRO GLY SER ALA PHE THR LEU SER          
SEQRES  10 A  214  THR PRO SER ALA PRO GLY PRO LEU VAL PHE THR ALA LEU          
SEQRES  11 A  214  SER PRO ASP SER LEU GLN LEU SER TRP GLU ARG PRO ARG          
SEQRES  12 A  214  ARG PRO ASN GLY ASP ILE VAL GLY TYR LEU VAL THR CYS          
SEQRES  13 A  214  GLU MET ALA GLN GLY GLY GLY PRO ALA THR ALA PHE ARG          
SEQRES  14 A  214  VAL ASP GLY ASP SER PRO GLU SER ARG LEU THR VAL PRO          
SEQRES  15 A  214  GLY LEU SER GLU ASN VAL PRO TYR LYS PHE LYS VAL GLN          
SEQRES  16 A  214  ALA ARG THR THR GLU GLY PHE GLY PRO GLU ARG GLU GLY          
SEQRES  17 A  214  ILE ILE THR ILE GLU SER                                      
SEQRES   1 B   30  ASP SER ASN GLU ASN LEU LEU LEU VAL HIS CYS GLY PRO          
SEQRES   2 B   30  THR LEU ILE ASN SER CYS ILE SER PHE GLY SER GLU SER          
SEQRES   3 B   30  PHE ASP GLY HIS                                              
FORMUL   3  HOH   *46(H2 O)                                                     
SHEET    1 AA1 8 SER A1514  VAL A1517  0                                        
SHEET    2 AA1 8 SER A1473  GLN A1479 -1  N  VAL A1476   O  VAL A1515           
SHEET    3 AA1 8 ARG A1463  GLY A1470 -1  N  VAL A1465   O  SER A1477           
SHEET    4 AA1 8 CYS B  44  GLY B  48 -1  O  ILE B  45   N  PHE A1466           
SHEET    5 AA1 8 PHE A1654  GLU A1665 -1  N  GLU A1659   O  CYS B  44           
SHEET    6 AA1 8 PRO A1641  THR A1650 -1  N  PHE A1644   O  GLY A1660           
SHEET    7 AA1 8 ILE A1601  MET A1610 -1  N  VAL A1602   O  ARG A1649           
SHEET    8 AA1 8 THR A1618  ASP A1623 -1  O  PHE A1620   N  VAL A1606           
SHEET    1 AA2 6 SER A1514  VAL A1517  0                                        
SHEET    2 AA2 6 SER A1473  GLN A1479 -1  N  VAL A1476   O  VAL A1515           
SHEET    3 AA2 6 ARG A1463  GLY A1470 -1  N  VAL A1465   O  SER A1477           
SHEET    4 AA2 6 CYS B  44  GLY B  48 -1  O  ILE B  45   N  PHE A1466           
SHEET    5 AA2 6 PHE A1654  GLU A1665 -1  N  GLU A1659   O  CYS B  44           
SHEET    6 AA2 6 GLU B  29  LEU B  33  1  O  LEU B  32   N  THR A1663           
SHEET    1 AA3 5 HIS A1503  ILE A1507  0                                        
SHEET    2 AA3 5 GLY A1489  LEU A1496 -1  N  VAL A1492   O  LEU A1505           
SHEET    3 AA3 5 TYR A1526  GLN A1533 -1  O  ARG A1529   N  GLU A1493           
SHEET    4 AA3 5 ARG A1542  SER A1550 -1  O  GLY A1544   N  PHE A1528           
SHEET    5 AA3 5 PHE A1566  SER A1569 -1  O  THR A1567   N  GLU A1549           
SHEET    1 AA4 4 GLU A1628  VAL A1633  0                                        
SHEET    2 AA4 4 SER A1586  GLU A1592 -1  N  LEU A1589   O  LEU A1631           
SHEET    3 AA4 4 VAL A1578  SER A1583 -1  N  VAL A1578   O  SER A1590           
SHEET    4 AA4 4 THR B  39  LEU B  40 -1  O  THR B  39   N  PHE A1579           
CRYST1  104.520   60.730   40.780  90.00 113.71  90.00 C 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009568  0.000000  0.004201        0.00000                         
SCALE2      0.000000  0.016466  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.026782        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system