GenomeNet

Database: PDB
Entry: 6HUG
LinkDB: 6HUG
Original site: 6HUG 
HEADER    MEMBRANE PROTEIN                        08-OCT-18   6HUG              
TITLE     CRYOEM STRUCTURE OF HUMAN FULL-LENGTH ALPHA1BETA3GAMMA2L GABA(A)R IN  
TITLE    2 COMPLEX WITH PICROTOXIN AND MEGABODY MB38.                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT ALPHA-1;          
COMPND   3 CHAIN: A, D;                                                         
COMPND   4 SYNONYM: GABA(A) RECEPTOR SUBUNIT ALPHA-1;                           
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 OTHER_DETAILS: POTENTIAL SIGNAL PEPTIDE: MKKSPGLSDY LWAWTLFLST       
COMPND   7 LTGRSYG FLAG TAG: DYKDDDDK;                                          
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT BETA-3;           
COMPND  10 CHAIN: B, E;                                                         
COMPND  11 SYNONYM: GABA(A) RECEPTOR SUBUNIT BETA-3;                            
COMPND  12 ENGINEERED: YES;                                                     
COMPND  13 MOL_ID: 3;                                                           
COMPND  14 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT GAMMA-2;          
COMPND  15 CHAIN: C;                                                            
COMPND  16 SYNONYM: GABA(A) RECEPTOR SUBUNIT GAMMA-2;                           
COMPND  17 ENGINEERED: YES;                                                     
COMPND  18 OTHER_DETAILS: LINKER SEQUENCE: GGSGGSGGSGK 1D4 TAG: TETSQVAPA;      
COMPND  19 MOL_ID: 4;                                                           
COMPND  20 MOLECULE: MEGABODY MB38;                                             
COMPND  21 CHAIN: G;                                                            
COMPND  22 ENGINEERED: YES;                                                     
COMPND  23 OTHER_DETAILS: 6HIS TAG: HHHHHH EPEA TAG: EPEA                       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: BOS TAURUS;                                     
SOURCE   3 ORGANISM_COMMON: BOVINE;                                             
SOURCE   4 ORGANISM_TAXID: 9913;                                                
SOURCE   5 GENE: GABRA1;                                                        
SOURCE   6 EXPRESSION_SYSTEM: HOMO SAPIENS;                                     
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 9606;                                       
SOURCE   8 EXPRESSION_SYSTEM_CELL_LINE: HEK293SGNTI-;                           
SOURCE   9 EXPRESSION_SYSTEM_ATCC_NUMBER: 3022;                                 
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_COMMON: HUMAN;                                              
SOURCE  13 ORGANISM_TAXID: 9606;                                                
SOURCE  14 GENE: GABRB3;                                                        
SOURCE  15 EXPRESSION_SYSTEM: HOMO SAPIENS;                                     
SOURCE  16 EXPRESSION_SYSTEM_TAXID: 9606;                                       
SOURCE  17 EXPRESSION_SYSTEM_CELL_LINE: HEK293SGNTI-;                           
SOURCE  18 EXPRESSION_SYSTEM_ATCC_NUMBER: 3022;                                 
SOURCE  19 MOL_ID: 3;                                                           
SOURCE  20 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  21 ORGANISM_COMMON: HUMAN;                                              
SOURCE  22 ORGANISM_TAXID: 9606;                                                
SOURCE  23 GENE: GABRG2;                                                        
SOURCE  24 EXPRESSION_SYSTEM: HOMO SAPIENS;                                     
SOURCE  25 EXPRESSION_SYSTEM_TAXID: 9606;                                       
SOURCE  26 EXPRESSION_SYSTEM_CELL_LINE: HEK293SGNTI-;                           
SOURCE  27 EXPRESSION_SYSTEM_ATCC_NUMBER: 3022;                                 
SOURCE  28 MOL_ID: 4;                                                           
SOURCE  29 ORGANISM_SCIENTIFIC: LAMA GLAMA;                                     
SOURCE  30 ORGANISM_TAXID: 9844;                                                
SOURCE  31 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  32 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    GABAAR, PTX, MEMBRANE, CHANNEL, NANOBODY, MEGABODY, CYS-LOOP, PLGIC,  
KEYWDS   2 INHIBITION, SIGNALLING, CNS, NEURONS, CHLORIDE, ION, GABA,           
KEYWDS   3 PICROTOXIN, MEMBRANE PROTEIN                                         
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    S.MASIULIS,R.DESAI,T.UCHANSKI,I.SERNA MARTIN,D.LAVERTY,D.KARIA,       
AUTHOR   2 T.MALINAUSKAS,Z.JASENKO,E.PARDON,A.KOTECHA,J.STEYAERT,K.W.MILLER,    
AUTHOR   3 A.R.ARICESCU                                                         
REVDAT   3   23-JAN-19 6HUG    1       JRNL                                     
REVDAT   2   16-JAN-19 6HUG    1       JRNL                                     
REVDAT   1   02-JAN-19 6HUG    0                                                
JRNL        AUTH   S.MASIULIS,R.DESAI,T.UCHANSKI,I.SERNA MARTIN,D.LAVERTY,      
JRNL        AUTH 2 D.KARIA,T.MALINAUSKAS,J.ZIVANOV,E.PARDON,A.KOTECHA,          
JRNL        AUTH 3 J.STEYAERT,K.W.MILLER,A.R.ARICESCU                           
JRNL        TITL   GABAARECEPTOR SIGNALLING MECHANISMS REVEALED BY STRUCTURAL   
JRNL        TITL 2 PHARMACOLOGY.                                                
JRNL        REF    NATURE                        V. 565   454 2019              
JRNL        REFN                   ISSN 0028-0836                               
JRNL        PMID   30602790                                                     
JRNL        DOI    10.1038/S41586-018-0832-5                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.10 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : EPU, GCTF, UCSF CHIMERA, RELION,          
REMARK   3                            RELION, RELION, RELION, PHENIX            
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : NULL                                
REMARK   3   REFINEMENT SPACE             : NULL                                
REMARK   3   REFINEMENT PROTOCOL          : FLEXIBLE FIT                        
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : NULL                                             
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 3.100                          
REMARK   3   NUMBER OF PARTICLES               : 56269                          
REMARK   3   CTF CORRECTION METHOD             : PHASE FLIPPING AND AMPLITUDE   
REMARK   3                                       CORRECTION                     
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 6HUG COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 08-OCT-18.                  
REMARK 100 THE DEPOSITION ID IS D_1200012207.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : NULL                              
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : HUMAN FULL-LENGTH HETEROMERIC     
REMARK 245                                    ALPHA1BETA3GAMMA2L GABA(A)R IN    
REMARK 245                                    COMPLEX WITH PICROTOXIN AND       
REMARK 245                                    MEGABODY MB38.; HUMAN FULL-       
REMARK 245                                    LENGTH HETEROMERIC                
REMARK 245                                    ALPHA1BETA3GAMMA2L GABA(A)R IN    
REMARK 245                                    COMPLEX WITH PICROTOXIN AND       
REMARK 245                                    MEGABODY MB38.; HUMAN FULL-       
REMARK 245                                    LENGTH HETEROMERIC                
REMARK 245                                    ALPHA1BETA3GAMMA2L GABA(A)R IN    
REMARK 245                                    COMPLEX WITH PICROTOXIN AND       
REMARK 245                                    MEGABODY MB38.                    
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 0.10                              
REMARK 245   SAMPLE SUPPORT DETAILS         : 3.5 UL OF 0.1 MG/ML PROTEIN       
REMARK 245                                    SOLUTION WAS APPLIED ON A GRID    
REMARK 245                                    IN THE VITROBOT MKIV CHAMBER      
REMARK 245                                    SET TO 95% RH AT 14.5 DEGC FOR    
REMARK 245                                    30S AND THEN BLOTTED FOR 5.5 S    
REMARK 245                                    AND PLUNGED                       
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.60                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : NULL                           
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : NULL                           
REMARK 245   MICROSCOPE MODEL                  : FEI TITAN KRIOS                
REMARK 245   DETECTOR TYPE                     : FEI FALCON III (4K X 4K)       
REMARK 245   MINIMUM DEFOCUS (NM)              : 500.00                         
REMARK 245   MAXIMUM DEFOCUS (NM)              : 700.00                         
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : 2.70                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 30.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : 75000                          
REMARK 245   CALIBRATED MAGNIFICATION          : NULL                           
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 300                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: HEXAMERIC                         
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: HEXAMERIC                  
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 41570 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 72630 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -79.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, G                      
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ASP A    -7                                                      
REMARK 465     TYR A    -6                                                      
REMARK 465     LYS A    -5                                                      
REMARK 465     ASP A    -4                                                      
REMARK 465     ASP A    -3                                                      
REMARK 465     ASP A    -2                                                      
REMARK 465     ASP A    -1                                                      
REMARK 465     LYS A     0                                                      
REMARK 465     GLN A     1                                                      
REMARK 465     PRO A     2                                                      
REMARK 465     SER A     3                                                      
REMARK 465     LEU A     4                                                      
REMARK 465     GLN A     5                                                      
REMARK 465     ASP A     6                                                      
REMARK 465     GLU A     7                                                      
REMARK 465     LEU A     8                                                      
REMARK 465     LYS A     9                                                      
REMARK 465     VAL A   322                                                      
REMARK 465     VAL A   323                                                      
REMARK 465     PRO A   324                                                      
REMARK 465     GLU A   325                                                      
REMARK 465     LYS A   326                                                      
REMARK 465     PRO A   327                                                      
REMARK 465     LYS A   328                                                      
REMARK 465     LYS A   329                                                      
REMARK 465     VAL A   330                                                      
REMARK 465     LYS A   331                                                      
REMARK 465     ASP A   332                                                      
REMARK 465     PRO A   333                                                      
REMARK 465     LEU A   334                                                      
REMARK 465     ILE A   335                                                      
REMARK 465     LYS A   336                                                      
REMARK 465     LYS A   337                                                      
REMARK 465     ASN A   338                                                      
REMARK 465     ASN A   339                                                      
REMARK 465     THR A   340                                                      
REMARK 465     TYR A   341                                                      
REMARK 465     ALA A   342                                                      
REMARK 465     PRO A   343                                                      
REMARK 465     THR A   344                                                      
REMARK 465     ALA A   345                                                      
REMARK 465     THR A   346                                                      
REMARK 465     SER A   347                                                      
REMARK 465     TYR A   348                                                      
REMARK 465     THR A   349                                                      
REMARK 465     PRO A   350                                                      
REMARK 465     ASN A   351                                                      
REMARK 465     LEU A   352                                                      
REMARK 465     ALA A   353                                                      
REMARK 465     ARG A   354                                                      
REMARK 465     GLY A   355                                                      
REMARK 465     ASP A   356                                                      
REMARK 465     PRO A   357                                                      
REMARK 465     GLY A   358                                                      
REMARK 465     LEU A   359                                                      
REMARK 465     ALA A   360                                                      
REMARK 465     THR A   361                                                      
REMARK 465     ILE A   362                                                      
REMARK 465     ALA A   363                                                      
REMARK 465     LYS A   364                                                      
REMARK 465     SER A   365                                                      
REMARK 465     ALA A   366                                                      
REMARK 465     THR A   367                                                      
REMARK 465     ILE A   368                                                      
REMARK 465     GLU A   369                                                      
REMARK 465     PRO A   370                                                      
REMARK 465     LYS A   371                                                      
REMARK 465     GLU A   372                                                      
REMARK 465     VAL A   373                                                      
REMARK 465     LYS A   374                                                      
REMARK 465     PRO A   375                                                      
REMARK 465     GLU A   376                                                      
REMARK 465     THR A   377                                                      
REMARK 465     LYS A   378                                                      
REMARK 465     PRO A   379                                                      
REMARK 465     PRO A   380                                                      
REMARK 465     GLU A   381                                                      
REMARK 465     PRO A   382                                                      
REMARK 465     LYS A   383                                                      
REMARK 465     GLU A   419                                                      
REMARK 465     PRO A   420                                                      
REMARK 465     GLN A   421                                                      
REMARK 465     LEU A   422                                                      
REMARK 465     LYS A   423                                                      
REMARK 465     ALA A   424                                                      
REMARK 465     PRO A   425                                                      
REMARK 465     THR A   426                                                      
REMARK 465     PRO A   427                                                      
REMARK 465     HIS A   428                                                      
REMARK 465     GLN A   429                                                      
REMARK 465     MET B   -24                                                      
REMARK 465     CYS B   -23                                                      
REMARK 465     SER B   -22                                                      
REMARK 465     GLY B   -21                                                      
REMARK 465     LEU B   -20                                                      
REMARK 465     LEU B   -19                                                      
REMARK 465     GLU B   -18                                                      
REMARK 465     LEU B   -17                                                      
REMARK 465     LEU B   -16                                                      
REMARK 465     LEU B   -15                                                      
REMARK 465     PRO B   -14                                                      
REMARK 465     ILE B   -13                                                      
REMARK 465     TRP B   -12                                                      
REMARK 465     LEU B   -11                                                      
REMARK 465     SER B   -10                                                      
REMARK 465     TRP B    -9                                                      
REMARK 465     THR B    -8                                                      
REMARK 465     LEU B    -7                                                      
REMARK 465     GLY B    -6                                                      
REMARK 465     THR B    -5                                                      
REMARK 465     ARG B    -4                                                      
REMARK 465     GLY B    -3                                                      
REMARK 465     SER B    -2                                                      
REMARK 465     GLU B    -1                                                      
REMARK 465     PRO B     0                                                      
REMARK 465     ARG B     1                                                      
REMARK 465     SER B     2                                                      
REMARK 465     VAL B     3                                                      
REMARK 465     ASN B     4                                                      
REMARK 465     ASP B     5                                                      
REMARK 465     PRO B     6                                                      
REMARK 465     GLY B     7                                                      
REMARK 465     GLN B   314                                                      
REMARK 465     LYS B   315                                                      
REMARK 465     LYS B   316                                                      
REMARK 465     LEU B   317                                                      
REMARK 465     ALA B   318                                                      
REMARK 465     GLU B   319                                                      
REMARK 465     LYS B   320                                                      
REMARK 465     THR B   321                                                      
REMARK 465     ALA B   322                                                      
REMARK 465     LYS B   323                                                      
REMARK 465     ALA B   324                                                      
REMARK 465     LYS B   325                                                      
REMARK 465     ASN B   326                                                      
REMARK 465     ASP B   327                                                      
REMARK 465     ARG B   328                                                      
REMARK 465     SER B   329                                                      
REMARK 465     LYS B   330                                                      
REMARK 465     SER B   331                                                      
REMARK 465     GLU B   332                                                      
REMARK 465     SER B   333                                                      
REMARK 465     ASN B   334                                                      
REMARK 465     ARG B   335                                                      
REMARK 465     VAL B   336                                                      
REMARK 465     ASP B   337                                                      
REMARK 465     ALA B   338                                                      
REMARK 465     HIS B   339                                                      
REMARK 465     GLY B   340                                                      
REMARK 465     ASN B   341                                                      
REMARK 465     ILE B   342                                                      
REMARK 465     LEU B   343                                                      
REMARK 465     LEU B   344                                                      
REMARK 465     THR B   345                                                      
REMARK 465     SER B   346                                                      
REMARK 465     LEU B   347                                                      
REMARK 465     GLU B   348                                                      
REMARK 465     VAL B   349                                                      
REMARK 465     HIS B   350                                                      
REMARK 465     ASN B   351                                                      
REMARK 465     GLU B   352                                                      
REMARK 465     MET B   353                                                      
REMARK 465     ASN B   354                                                      
REMARK 465     GLU B   355                                                      
REMARK 465     VAL B   356                                                      
REMARK 465     SER B   357                                                      
REMARK 465     GLY B   358                                                      
REMARK 465     GLY B   359                                                      
REMARK 465     ILE B   360                                                      
REMARK 465     GLY B   361                                                      
REMARK 465     ASP B   362                                                      
REMARK 465     THR B   363                                                      
REMARK 465     ARG B   364                                                      
REMARK 465     ASN B   365                                                      
REMARK 465     SER B   366                                                      
REMARK 465     ALA B   367                                                      
REMARK 465     ILE B   368                                                      
REMARK 465     SER B   369                                                      
REMARK 465     PHE B   370                                                      
REMARK 465     ASP B   371                                                      
REMARK 465     ASN B   372                                                      
REMARK 465     SER B   373                                                      
REMARK 465     GLY B   374                                                      
REMARK 465     ILE B   375                                                      
REMARK 465     GLN B   376                                                      
REMARK 465     TYR B   377                                                      
REMARK 465     ARG B   378                                                      
REMARK 465     LYS B   379                                                      
REMARK 465     GLN B   380                                                      
REMARK 465     SER B   381                                                      
REMARK 465     MET B   382                                                      
REMARK 465     PRO B   383                                                      
REMARK 465     ARG B   384                                                      
REMARK 465     GLU B   385                                                      
REMARK 465     GLY B   386                                                      
REMARK 465     HIS B   387                                                      
REMARK 465     GLY B   388                                                      
REMARK 465     ARG B   389                                                      
REMARK 465     PHE B   390                                                      
REMARK 465     LEU B   391                                                      
REMARK 465     GLY B   392                                                      
REMARK 465     ASP B   393                                                      
REMARK 465     ARG B   394                                                      
REMARK 465     SER B   395                                                      
REMARK 465     LEU B   396                                                      
REMARK 465     PRO B   397                                                      
REMARK 465     HIS B   398                                                      
REMARK 465     LYS B   399                                                      
REMARK 465     LYS B   400                                                      
REMARK 465     THR B   401                                                      
REMARK 465     HIS B   402                                                      
REMARK 465     LEU B   403                                                      
REMARK 465     ARG B   404                                                      
REMARK 465     ARG B   405                                                      
REMARK 465     ARG B   406                                                      
REMARK 465     SER B   407                                                      
REMARK 465     SER B   408                                                      
REMARK 465     GLN B   409                                                      
REMARK 465     LEU B   410                                                      
REMARK 465     LYS B   411                                                      
REMARK 465     ILE B   412                                                      
REMARK 465     LYS B   413                                                      
REMARK 465     ILE B   414                                                      
REMARK 465     PRO B   415                                                      
REMARK 465     ASP B   416                                                      
REMARK 465     LEU B   417                                                      
REMARK 465     ASN B   448                                                      
REMARK 465     MET C   -38                                                      
REMARK 465     SER C   -37                                                      
REMARK 465     SER C   -36                                                      
REMARK 465     PRO C   -35                                                      
REMARK 465     ASN C   -34                                                      
REMARK 465     ILE C   -33                                                      
REMARK 465     TRP C   -32                                                      
REMARK 465     SER C   -31                                                      
REMARK 465     THR C   -30                                                      
REMARK 465     GLY C   -29                                                      
REMARK 465     SER C   -28                                                      
REMARK 465     SER C   -27                                                      
REMARK 465     VAL C   -26                                                      
REMARK 465     TYR C   -25                                                      
REMARK 465     SER C   -24                                                      
REMARK 465     THR C   -23                                                      
REMARK 465     PRO C   -22                                                      
REMARK 465     VAL C   -21                                                      
REMARK 465     PHE C   -20                                                      
REMARK 465     SER C   -19                                                      
REMARK 465     GLN C   -18                                                      
REMARK 465     LYS C   -17                                                      
REMARK 465     MET C   -16                                                      
REMARK 465     THR C   -15                                                      
REMARK 465     VAL C   -14                                                      
REMARK 465     TRP C   -13                                                      
REMARK 465     ILE C   -12                                                      
REMARK 465     LEU C   -11                                                      
REMARK 465     LEU C   -10                                                      
REMARK 465     LEU C    -9                                                      
REMARK 465     LEU C    -8                                                      
REMARK 465     SER C    -7                                                      
REMARK 465     LEU C    -6                                                      
REMARK 465     TYR C    -5                                                      
REMARK 465     PRO C    -4                                                      
REMARK 465     GLY C    -3                                                      
REMARK 465     PHE C    -2                                                      
REMARK 465     THR C    -1                                                      
REMARK 465     SER C     0                                                      
REMARK 465     GLN C     1                                                      
REMARK 465     LYS C     2                                                      
REMARK 465     SER C     3                                                      
REMARK 465     ASP C     4                                                      
REMARK 465     ASP C     5                                                      
REMARK 465     ASP C     6                                                      
REMARK 465     TYR C     7                                                      
REMARK 465     GLU C     8                                                      
REMARK 465     ASP C     9                                                      
REMARK 465     TYR C    10                                                      
REMARK 465     ALA C    11                                                      
REMARK 465     SER C    12                                                      
REMARK 465     ASN C    13                                                      
REMARK 465     LYS C    14                                                      
REMARK 465     THR C    15                                                      
REMARK 465     TRP C    16                                                      
REMARK 465     VAL C    17                                                      
REMARK 465     LEU C    18                                                      
REMARK 465     THR C    19                                                      
REMARK 465     PRO C    20                                                      
REMARK 465     LYS C    21                                                      
REMARK 465     VAL C    22                                                      
REMARK 465     PRO C    23                                                      
REMARK 465     GLU C    24                                                      
REMARK 465     GLY C    25                                                      
REMARK 465     LYS C   325                                                      
REMARK 465     PRO C   326                                                      
REMARK 465     SER C   327                                                      
REMARK 465     LYS C   328                                                      
REMARK 465     ASP C   329                                                      
REMARK 465     LYS C   330                                                      
REMARK 465     ASP C   331                                                      
REMARK 465     LYS C   332                                                      
REMARK 465     LYS C   333                                                      
REMARK 465     LYS C   334                                                      
REMARK 465     LYS C   335                                                      
REMARK 465     ASN C   336                                                      
REMARK 465     PRO C   337                                                      
REMARK 465     LEU C   338                                                      
REMARK 465     LEU C   339                                                      
REMARK 465     ARG C   340                                                      
REMARK 465     MET C   341                                                      
REMARK 465     PHE C   342                                                      
REMARK 465     SER C   343                                                      
REMARK 465     PHE C   344                                                      
REMARK 465     LYS C   345                                                      
REMARK 465     ALA C   346                                                      
REMARK 465     PRO C   347                                                      
REMARK 465     THR C   348                                                      
REMARK 465     ILE C   349                                                      
REMARK 465     ASP C   350                                                      
REMARK 465     ILE C   351                                                      
REMARK 465     ARG C   352                                                      
REMARK 465     PRO C   353                                                      
REMARK 465     ARG C   354                                                      
REMARK 465     SER C   355                                                      
REMARK 465     ALA C   356                                                      
REMARK 465     THR C   357                                                      
REMARK 465     ILE C   358                                                      
REMARK 465     GLN C   359                                                      
REMARK 465     MET C   360                                                      
REMARK 465     ASN C   361                                                      
REMARK 465     ASN C   362                                                      
REMARK 465     ALA C   363                                                      
REMARK 465     THR C   364                                                      
REMARK 465     HIS C   365                                                      
REMARK 465     LEU C   366                                                      
REMARK 465     GLN C   367                                                      
REMARK 465     GLU C   368                                                      
REMARK 465     ARG C   369                                                      
REMARK 465     ASP C   370                                                      
REMARK 465     GLU C   371                                                      
REMARK 465     GLU C   372                                                      
REMARK 465     TYR C   373                                                      
REMARK 465     GLY C   374                                                      
REMARK 465     TYR C   375                                                      
REMARK 465     GLU C   376                                                      
REMARK 465     CYS C   377                                                      
REMARK 465     LEU C   378                                                      
REMARK 465     ASP C   379                                                      
REMARK 465     GLY C   380                                                      
REMARK 465     LYS C   381                                                      
REMARK 465     ASP C   382                                                      
REMARK 465     CYS C   383                                                      
REMARK 465     ALA C   384                                                      
REMARK 465     SER C   385                                                      
REMARK 465     PHE C   386                                                      
REMARK 465     PHE C   387                                                      
REMARK 465     CYS C   388                                                      
REMARK 465     CYS C   389                                                      
REMARK 465     PHE C   390                                                      
REMARK 465     GLU C   391                                                      
REMARK 465     ASP C   392                                                      
REMARK 465     CYS C   393                                                      
REMARK 465     ARG C   394                                                      
REMARK 465     THR C   395                                                      
REMARK 465     GLY C   396                                                      
REMARK 465     ALA C   397                                                      
REMARK 465     TRP C   398                                                      
REMARK 465     ARG C   399                                                      
REMARK 465     HIS C   400                                                      
REMARK 465     GLY C   401                                                      
REMARK 465     ARG C   402                                                      
REMARK 465     ILE C   403                                                      
REMARK 465     HIS C   404                                                      
REMARK 465     ILE C   405                                                      
REMARK 465     GLY C   437                                                      
REMARK 465     GLY C   438                                                      
REMARK 465     SER C   439                                                      
REMARK 465     GLY C   440                                                      
REMARK 465     GLY C   441                                                      
REMARK 465     SER C   442                                                      
REMARK 465     GLY C   443                                                      
REMARK 465     GLY C   444                                                      
REMARK 465     SER C   445                                                      
REMARK 465     GLY C   446                                                      
REMARK 465     LYS C   447                                                      
REMARK 465     THR C   448                                                      
REMARK 465     GLU C   449                                                      
REMARK 465     THR C   450                                                      
REMARK 465     SER C   451                                                      
REMARK 465     GLN C   452                                                      
REMARK 465     VAL C   453                                                      
REMARK 465     ALA C   454                                                      
REMARK 465     PRO C   455                                                      
REMARK 465     ALA C   456                                                      
REMARK 465     ASP D    -7                                                      
REMARK 465     TYR D    -6                                                      
REMARK 465     LYS D    -5                                                      
REMARK 465     ASP D    -4                                                      
REMARK 465     ASP D    -3                                                      
REMARK 465     ASP D    -2                                                      
REMARK 465     ASP D    -1                                                      
REMARK 465     LYS D     0                                                      
REMARK 465     GLN D     1                                                      
REMARK 465     PRO D     2                                                      
REMARK 465     SER D     3                                                      
REMARK 465     LEU D     4                                                      
REMARK 465     GLN D     5                                                      
REMARK 465     ASP D     6                                                      
REMARK 465     GLU D     7                                                      
REMARK 465     LEU D     8                                                      
REMARK 465     SER D   321                                                      
REMARK 465     VAL D   322                                                      
REMARK 465     VAL D   323                                                      
REMARK 465     PRO D   324                                                      
REMARK 465     GLU D   325                                                      
REMARK 465     LYS D   326                                                      
REMARK 465     PRO D   327                                                      
REMARK 465     LYS D   328                                                      
REMARK 465     LYS D   329                                                      
REMARK 465     VAL D   330                                                      
REMARK 465     LYS D   331                                                      
REMARK 465     ASP D   332                                                      
REMARK 465     PRO D   333                                                      
REMARK 465     LEU D   334                                                      
REMARK 465     ILE D   335                                                      
REMARK 465     LYS D   336                                                      
REMARK 465     LYS D   337                                                      
REMARK 465     ASN D   338                                                      
REMARK 465     ASN D   339                                                      
REMARK 465     THR D   340                                                      
REMARK 465     TYR D   341                                                      
REMARK 465     ALA D   342                                                      
REMARK 465     PRO D   343                                                      
REMARK 465     THR D   344                                                      
REMARK 465     ALA D   345                                                      
REMARK 465     THR D   346                                                      
REMARK 465     SER D   347                                                      
REMARK 465     TYR D   348                                                      
REMARK 465     THR D   349                                                      
REMARK 465     PRO D   350                                                      
REMARK 465     ASN D   351                                                      
REMARK 465     LEU D   352                                                      
REMARK 465     ALA D   353                                                      
REMARK 465     ARG D   354                                                      
REMARK 465     GLY D   355                                                      
REMARK 465     ASP D   356                                                      
REMARK 465     PRO D   357                                                      
REMARK 465     GLY D   358                                                      
REMARK 465     LEU D   359                                                      
REMARK 465     ALA D   360                                                      
REMARK 465     THR D   361                                                      
REMARK 465     ILE D   362                                                      
REMARK 465     ALA D   363                                                      
REMARK 465     LYS D   364                                                      
REMARK 465     SER D   365                                                      
REMARK 465     ALA D   366                                                      
REMARK 465     THR D   367                                                      
REMARK 465     ILE D   368                                                      
REMARK 465     GLU D   369                                                      
REMARK 465     PRO D   370                                                      
REMARK 465     LYS D   371                                                      
REMARK 465     GLU D   372                                                      
REMARK 465     VAL D   373                                                      
REMARK 465     LYS D   374                                                      
REMARK 465     PRO D   375                                                      
REMARK 465     GLU D   376                                                      
REMARK 465     THR D   377                                                      
REMARK 465     LYS D   378                                                      
REMARK 465     PRO D   379                                                      
REMARK 465     PRO D   380                                                      
REMARK 465     GLU D   381                                                      
REMARK 465     PRO D   382                                                      
REMARK 465     LYS D   383                                                      
REMARK 465     GLU D   419                                                      
REMARK 465     PRO D   420                                                      
REMARK 465     GLN D   421                                                      
REMARK 465     LEU D   422                                                      
REMARK 465     LYS D   423                                                      
REMARK 465     ALA D   424                                                      
REMARK 465     PRO D   425                                                      
REMARK 465     THR D   426                                                      
REMARK 465     PRO D   427                                                      
REMARK 465     HIS D   428                                                      
REMARK 465     GLN D   429                                                      
REMARK 465     MET E   -24                                                      
REMARK 465     CYS E   -23                                                      
REMARK 465     SER E   -22                                                      
REMARK 465     GLY E   -21                                                      
REMARK 465     LEU E   -20                                                      
REMARK 465     LEU E   -19                                                      
REMARK 465     GLU E   -18                                                      
REMARK 465     LEU E   -17                                                      
REMARK 465     LEU E   -16                                                      
REMARK 465     LEU E   -15                                                      
REMARK 465     PRO E   -14                                                      
REMARK 465     ILE E   -13                                                      
REMARK 465     TRP E   -12                                                      
REMARK 465     LEU E   -11                                                      
REMARK 465     SER E   -10                                                      
REMARK 465     TRP E    -9                                                      
REMARK 465     THR E    -8                                                      
REMARK 465     LEU E    -7                                                      
REMARK 465     GLY E    -6                                                      
REMARK 465     THR E    -5                                                      
REMARK 465     ARG E    -4                                                      
REMARK 465     GLY E    -3                                                      
REMARK 465     SER E    -2                                                      
REMARK 465     GLU E    -1                                                      
REMARK 465     PRO E     0                                                      
REMARK 465     ARG E     1                                                      
REMARK 465     SER E     2                                                      
REMARK 465     VAL E     3                                                      
REMARK 465     ASN E     4                                                      
REMARK 465     ASP E     5                                                      
REMARK 465     PRO E     6                                                      
REMARK 465     GLY E     7                                                      
REMARK 465     ARG E   313                                                      
REMARK 465     GLN E   314                                                      
REMARK 465     LYS E   315                                                      
REMARK 465     LYS E   316                                                      
REMARK 465     LEU E   317                                                      
REMARK 465     ALA E   318                                                      
REMARK 465     GLU E   319                                                      
REMARK 465     LYS E   320                                                      
REMARK 465     THR E   321                                                      
REMARK 465     ALA E   322                                                      
REMARK 465     LYS E   323                                                      
REMARK 465     ALA E   324                                                      
REMARK 465     LYS E   325                                                      
REMARK 465     ASN E   326                                                      
REMARK 465     ASP E   327                                                      
REMARK 465     ARG E   328                                                      
REMARK 465     SER E   329                                                      
REMARK 465     LYS E   330                                                      
REMARK 465     SER E   331                                                      
REMARK 465     GLU E   332                                                      
REMARK 465     SER E   333                                                      
REMARK 465     ASN E   334                                                      
REMARK 465     ARG E   335                                                      
REMARK 465     VAL E   336                                                      
REMARK 465     ASP E   337                                                      
REMARK 465     ALA E   338                                                      
REMARK 465     HIS E   339                                                      
REMARK 465     GLY E   340                                                      
REMARK 465     ASN E   341                                                      
REMARK 465     ILE E   342                                                      
REMARK 465     LEU E   343                                                      
REMARK 465     LEU E   344                                                      
REMARK 465     THR E   345                                                      
REMARK 465     SER E   346                                                      
REMARK 465     LEU E   347                                                      
REMARK 465     GLU E   348                                                      
REMARK 465     VAL E   349                                                      
REMARK 465     HIS E   350                                                      
REMARK 465     ASN E   351                                                      
REMARK 465     GLU E   352                                                      
REMARK 465     MET E   353                                                      
REMARK 465     ASN E   354                                                      
REMARK 465     GLU E   355                                                      
REMARK 465     VAL E   356                                                      
REMARK 465     SER E   357                                                      
REMARK 465     GLY E   358                                                      
REMARK 465     GLY E   359                                                      
REMARK 465     ILE E   360                                                      
REMARK 465     GLY E   361                                                      
REMARK 465     ASP E   362                                                      
REMARK 465     THR E   363                                                      
REMARK 465     ARG E   364                                                      
REMARK 465     ASN E   365                                                      
REMARK 465     SER E   366                                                      
REMARK 465     ALA E   367                                                      
REMARK 465     ILE E   368                                                      
REMARK 465     SER E   369                                                      
REMARK 465     PHE E   370                                                      
REMARK 465     ASP E   371                                                      
REMARK 465     ASN E   372                                                      
REMARK 465     SER E   373                                                      
REMARK 465     GLY E   374                                                      
REMARK 465     ILE E   375                                                      
REMARK 465     GLN E   376                                                      
REMARK 465     TYR E   377                                                      
REMARK 465     ARG E   378                                                      
REMARK 465     LYS E   379                                                      
REMARK 465     GLN E   380                                                      
REMARK 465     SER E   381                                                      
REMARK 465     MET E   382                                                      
REMARK 465     PRO E   383                                                      
REMARK 465     ARG E   384                                                      
REMARK 465     GLU E   385                                                      
REMARK 465     GLY E   386                                                      
REMARK 465     HIS E   387                                                      
REMARK 465     GLY E   388                                                      
REMARK 465     ARG E   389                                                      
REMARK 465     PHE E   390                                                      
REMARK 465     LEU E   391                                                      
REMARK 465     GLY E   392                                                      
REMARK 465     ASP E   393                                                      
REMARK 465     ARG E   394                                                      
REMARK 465     SER E   395                                                      
REMARK 465     LEU E   396                                                      
REMARK 465     PRO E   397                                                      
REMARK 465     HIS E   398                                                      
REMARK 465     LYS E   399                                                      
REMARK 465     LYS E   400                                                      
REMARK 465     THR E   401                                                      
REMARK 465     HIS E   402                                                      
REMARK 465     LEU E   403                                                      
REMARK 465     ARG E   404                                                      
REMARK 465     ARG E   405                                                      
REMARK 465     ARG E   406                                                      
REMARK 465     SER E   407                                                      
REMARK 465     SER E   408                                                      
REMARK 465     GLN E   409                                                      
REMARK 465     LEU E   410                                                      
REMARK 465     LYS E   411                                                      
REMARK 465     ILE E   412                                                      
REMARK 465     LYS E   413                                                      
REMARK 465     ILE E   414                                                      
REMARK 465     PRO E   415                                                      
REMARK 465     ASP E   416                                                      
REMARK 465     LEU E   417                                                      
REMARK 465     THR E   418                                                      
REMARK 465     ASN E   448                                                      
REMARK 465     THR G    14                                                      
REMARK 465     LYS G    15                                                      
REMARK 465     THR G    16                                                      
REMARK 465     THR G    17                                                      
REMARK 465     THR G    18                                                      
REMARK 465     SER G    19                                                      
REMARK 465     VAL G    20                                                      
REMARK 465     ILE G    21                                                      
REMARK 465     ASP G    22                                                      
REMARK 465     THR G    23                                                      
REMARK 465     THR G    24                                                      
REMARK 465     ASN G    25                                                      
REMARK 465     ASP G    26                                                      
REMARK 465     ALA G    27                                                      
REMARK 465     GLN G    28                                                      
REMARK 465     ASN G    29                                                      
REMARK 465     LEU G    30                                                      
REMARK 465     LEU G    31                                                      
REMARK 465     THR G    32                                                      
REMARK 465     GLN G    33                                                      
REMARK 465     ALA G    34                                                      
REMARK 465     GLN G    35                                                      
REMARK 465     THR G    36                                                      
REMARK 465     ILE G    37                                                      
REMARK 465     VAL G    38                                                      
REMARK 465     ASN G    39                                                      
REMARK 465     THR G    40                                                      
REMARK 465     LEU G    41                                                      
REMARK 465     LYS G    42                                                      
REMARK 465     ASP G    43                                                      
REMARK 465     TYR G    44                                                      
REMARK 465     CYS G    45                                                      
REMARK 465     PRO G    46                                                      
REMARK 465     ILE G    47                                                      
REMARK 465     LEU G    48                                                      
REMARK 465     ILE G    49                                                      
REMARK 465     ALA G    50                                                      
REMARK 465     LYS G    51                                                      
REMARK 465     SER G    52                                                      
REMARK 465     SER G    53                                                      
REMARK 465     SER G    54                                                      
REMARK 465     SER G    55                                                      
REMARK 465     ASN G    56                                                      
REMARK 465     GLY G    57                                                      
REMARK 465     GLY G    58                                                      
REMARK 465     THR G    59                                                      
REMARK 465     ASN G    60                                                      
REMARK 465     ASN G    61                                                      
REMARK 465     ALA G    62                                                      
REMARK 465     ASN G    63                                                      
REMARK 465     THR G    64                                                      
REMARK 465     PRO G    65                                                      
REMARK 465     SER G    66                                                      
REMARK 465     TRP G    67                                                      
REMARK 465     GLN G    68                                                      
REMARK 465     THR G    69                                                      
REMARK 465     ALA G    70                                                      
REMARK 465     GLY G    71                                                      
REMARK 465     GLY G    72                                                      
REMARK 465     GLY G    73                                                      
REMARK 465     LYS G    74                                                      
REMARK 465     ASN G    75                                                      
REMARK 465     SER G    76                                                      
REMARK 465     CYS G    77                                                      
REMARK 465     ALA G    78                                                      
REMARK 465     THR G    79                                                      
REMARK 465     PHE G    80                                                      
REMARK 465     GLY G    81                                                      
REMARK 465     ALA G    82                                                      
REMARK 465     GLU G    83                                                      
REMARK 465     PHE G    84                                                      
REMARK 465     SER G    85                                                      
REMARK 465     ALA G    86                                                      
REMARK 465     ALA G    87                                                      
REMARK 465     SER G    88                                                      
REMARK 465     ASP G    89                                                      
REMARK 465     MET G    90                                                      
REMARK 465     ILE G    91                                                      
REMARK 465     ASN G    92                                                      
REMARK 465     ASN G    93                                                      
REMARK 465     ALA G    94                                                      
REMARK 465     GLN G    95                                                      
REMARK 465     LYS G    96                                                      
REMARK 465     ILE G    97                                                      
REMARK 465     VAL G    98                                                      
REMARK 465     GLN G    99                                                      
REMARK 465     GLU G   100                                                      
REMARK 465     THR G   101                                                      
REMARK 465     GLN G   102                                                      
REMARK 465     GLN G   103                                                      
REMARK 465     LEU G   104                                                      
REMARK 465     SER G   105                                                      
REMARK 465     ALA G   106                                                      
REMARK 465     ASN G   107                                                      
REMARK 465     GLN G   108                                                      
REMARK 465     PRO G   109                                                      
REMARK 465     LYS G   110                                                      
REMARK 465     ASN G   111                                                      
REMARK 465     ILE G   112                                                      
REMARK 465     THR G   113                                                      
REMARK 465     GLN G   114                                                      
REMARK 465     PRO G   115                                                      
REMARK 465     HIS G   116                                                      
REMARK 465     ASN G   117                                                      
REMARK 465     LEU G   118                                                      
REMARK 465     ASN G   119                                                      
REMARK 465     LEU G   120                                                      
REMARK 465     ASN G   121                                                      
REMARK 465     SER G   122                                                      
REMARK 465     PRO G   123                                                      
REMARK 465     SER G   124                                                      
REMARK 465     SER G   125                                                      
REMARK 465     LEU G   126                                                      
REMARK 465     THR G   127                                                      
REMARK 465     ALA G   128                                                      
REMARK 465     LEU G   129                                                      
REMARK 465     ALA G   130                                                      
REMARK 465     GLN G   131                                                      
REMARK 465     LYS G   132                                                      
REMARK 465     MET G   133                                                      
REMARK 465     LEU G   134                                                      
REMARK 465     LYS G   135                                                      
REMARK 465     ASN G   136                                                      
REMARK 465     ALA G   137                                                      
REMARK 465     GLN G   138                                                      
REMARK 465     SER G   139                                                      
REMARK 465     GLN G   140                                                      
REMARK 465     ALA G   141                                                      
REMARK 465     GLU G   142                                                      
REMARK 465     ILE G   143                                                      
REMARK 465     LEU G   144                                                      
REMARK 465     LYS G   145                                                      
REMARK 465     LEU G   146                                                      
REMARK 465     ALA G   147                                                      
REMARK 465     ASN G   148                                                      
REMARK 465     GLN G   149                                                      
REMARK 465     VAL G   150                                                      
REMARK 465     GLU G   151                                                      
REMARK 465     SER G   152                                                      
REMARK 465     ASP G   153                                                      
REMARK 465     PHE G   154                                                      
REMARK 465     ASN G   155                                                      
REMARK 465     LYS G   156                                                      
REMARK 465     LEU G   157                                                      
REMARK 465     SER G   158                                                      
REMARK 465     SER G   159                                                      
REMARK 465     GLY G   160                                                      
REMARK 465     HIS G   161                                                      
REMARK 465     LEU G   162                                                      
REMARK 465     LYS G   163                                                      
REMARK 465     ASP G   164                                                      
REMARK 465     TYR G   165                                                      
REMARK 465     ILE G   166                                                      
REMARK 465     GLY G   167                                                      
REMARK 465     LYS G   168                                                      
REMARK 465     CYS G   169                                                      
REMARK 465     ASP G   170                                                      
REMARK 465     ALA G   171                                                      
REMARK 465     SER G   172                                                      
REMARK 465     ALA G   173                                                      
REMARK 465     ILE G   174                                                      
REMARK 465     SER G   175                                                      
REMARK 465     SER G   176                                                      
REMARK 465     ALA G   177                                                      
REMARK 465     ASN G   178                                                      
REMARK 465     MET G   179                                                      
REMARK 465     THR G   180                                                      
REMARK 465     MET G   181                                                      
REMARK 465     GLN G   182                                                      
REMARK 465     ASN G   183                                                      
REMARK 465     GLN G   184                                                      
REMARK 465     LYS G   185                                                      
REMARK 465     ASN G   186                                                      
REMARK 465     ASN G   187                                                      
REMARK 465     TRP G   188                                                      
REMARK 465     GLY G   189                                                      
REMARK 465     ASN G   190                                                      
REMARK 465     GLY G   191                                                      
REMARK 465     CYS G   192                                                      
REMARK 465     ALA G   193                                                      
REMARK 465     GLY G   194                                                      
REMARK 465     VAL G   195                                                      
REMARK 465     GLU G   196                                                      
REMARK 465     GLU G   197                                                      
REMARK 465     THR G   198                                                      
REMARK 465     GLN G   199                                                      
REMARK 465     SER G   200                                                      
REMARK 465     LEU G   201                                                      
REMARK 465     LEU G   202                                                      
REMARK 465     LYS G   203                                                      
REMARK 465     THR G   204                                                      
REMARK 465     SER G   205                                                      
REMARK 465     ALA G   206                                                      
REMARK 465     ALA G   207                                                      
REMARK 465     ASP G   208                                                      
REMARK 465     PHE G   209                                                      
REMARK 465     ASN G   210                                                      
REMARK 465     ASN G   211                                                      
REMARK 465     GLN G   212                                                      
REMARK 465     THR G   213                                                      
REMARK 465     PRO G   214                                                      
REMARK 465     GLN G   215                                                      
REMARK 465     ILE G   216                                                      
REMARK 465     ASN G   217                                                      
REMARK 465     GLN G   218                                                      
REMARK 465     ALA G   219                                                      
REMARK 465     GLN G   220                                                      
REMARK 465     ASN G   221                                                      
REMARK 465     LEU G   222                                                      
REMARK 465     ALA G   223                                                      
REMARK 465     ASN G   224                                                      
REMARK 465     THR G   225                                                      
REMARK 465     LEU G   226                                                      
REMARK 465     ILE G   227                                                      
REMARK 465     GLN G   228                                                      
REMARK 465     GLU G   229                                                      
REMARK 465     LEU G   230                                                      
REMARK 465     GLY G   231                                                      
REMARK 465     ASN G   232                                                      
REMARK 465     ASN G   233                                                      
REMARK 465     PRO G   234                                                      
REMARK 465     PHE G   235                                                      
REMARK 465     ARG G   236                                                      
REMARK 465     ALA G   237                                                      
REMARK 465     SER G   238                                                      
REMARK 465     GLY G   239                                                      
REMARK 465     GLY G   240                                                      
REMARK 465     GLY G   241                                                      
REMARK 465     SER G   242                                                      
REMARK 465     GLY G   243                                                      
REMARK 465     GLY G   244                                                      
REMARK 465     GLY G   245                                                      
REMARK 465     GLY G   246                                                      
REMARK 465     SER G   247                                                      
REMARK 465     GLY G   248                                                      
REMARK 465     LYS G   249                                                      
REMARK 465     LEU G   250                                                      
REMARK 465     SER G   251                                                      
REMARK 465     ASP G   252                                                      
REMARK 465     THR G   253                                                      
REMARK 465     TYR G   254                                                      
REMARK 465     GLU G   255                                                      
REMARK 465     GLN G   256                                                      
REMARK 465     LEU G   257                                                      
REMARK 465     SER G   258                                                      
REMARK 465     ARG G   259                                                      
REMARK 465     LEU G   260                                                      
REMARK 465     LEU G   261                                                      
REMARK 465     THR G   262                                                      
REMARK 465     ASN G   263                                                      
REMARK 465     ASP G   264                                                      
REMARK 465     ASN G   265                                                      
REMARK 465     GLY G   266                                                      
REMARK 465     THR G   267                                                      
REMARK 465     ASN G   268                                                      
REMARK 465     SER G   269                                                      
REMARK 465     LYS G   270                                                      
REMARK 465     THR G   271                                                      
REMARK 465     SER G   272                                                      
REMARK 465     ALA G   273                                                      
REMARK 465     GLN G   274                                                      
REMARK 465     ALA G   275                                                      
REMARK 465     ILE G   276                                                      
REMARK 465     ASN G   277                                                      
REMARK 465     GLN G   278                                                      
REMARK 465     ALA G   279                                                      
REMARK 465     VAL G   280                                                      
REMARK 465     ASN G   281                                                      
REMARK 465     ASN G   282                                                      
REMARK 465     LEU G   283                                                      
REMARK 465     ASN G   284                                                      
REMARK 465     GLU G   285                                                      
REMARK 465     ARG G   286                                                      
REMARK 465     ALA G   287                                                      
REMARK 465     LYS G   288                                                      
REMARK 465     THR G   289                                                      
REMARK 465     LEU G   290                                                      
REMARK 465     ALA G   291                                                      
REMARK 465     GLY G   292                                                      
REMARK 465     GLY G   293                                                      
REMARK 465     THR G   294                                                      
REMARK 465     THR G   295                                                      
REMARK 465     ASN G   296                                                      
REMARK 465     SER G   297                                                      
REMARK 465     PRO G   298                                                      
REMARK 465     ALA G   299                                                      
REMARK 465     TYR G   300                                                      
REMARK 465     GLN G   301                                                      
REMARK 465     ALA G   302                                                      
REMARK 465     THR G   303                                                      
REMARK 465     LEU G   304                                                      
REMARK 465     LEU G   305                                                      
REMARK 465     ALA G   306                                                      
REMARK 465     LEU G   307                                                      
REMARK 465     ARG G   308                                                      
REMARK 465     SER G   309                                                      
REMARK 465     VAL G   310                                                      
REMARK 465     LEU G   311                                                      
REMARK 465     GLY G   312                                                      
REMARK 465     LEU G   313                                                      
REMARK 465     TRP G   314                                                      
REMARK 465     ASN G   315                                                      
REMARK 465     SER G   316                                                      
REMARK 465     MET G   317                                                      
REMARK 465     GLY G   318                                                      
REMARK 465     TYR G   319                                                      
REMARK 465     ALA G   320                                                      
REMARK 465     VAL G   321                                                      
REMARK 465     ILE G   322                                                      
REMARK 465     CYS G   323                                                      
REMARK 465     GLY G   324                                                      
REMARK 465     GLY G   325                                                      
REMARK 465     TYR G   326                                                      
REMARK 465     THR G   327                                                      
REMARK 465     LYS G   328                                                      
REMARK 465     SER G   329                                                      
REMARK 465     PRO G   330                                                      
REMARK 465     GLY G   331                                                      
REMARK 465     GLU G   332                                                      
REMARK 465     ASN G   333                                                      
REMARK 465     ASN G   334                                                      
REMARK 465     GLN G   335                                                      
REMARK 465     LYS G   336                                                      
REMARK 465     ASP G   337                                                      
REMARK 465     PHE G   338                                                      
REMARK 465     HIS G   339                                                      
REMARK 465     TYR G   340                                                      
REMARK 465     THR G   341                                                      
REMARK 465     ASP G   342                                                      
REMARK 465     GLU G   343                                                      
REMARK 465     ASN G   344                                                      
REMARK 465     GLY G   345                                                      
REMARK 465     ASN G   346                                                      
REMARK 465     GLY G   347                                                      
REMARK 465     THR G   348                                                      
REMARK 465     THR G   349                                                      
REMARK 465     ILE G   350                                                      
REMARK 465     ASN G   351                                                      
REMARK 465     CYS G   352                                                      
REMARK 465     GLY G   353                                                      
REMARK 465     GLY G   354                                                      
REMARK 465     SER G   355                                                      
REMARK 465     THR G   356                                                      
REMARK 465     ASN G   357                                                      
REMARK 465     SER G   358                                                      
REMARK 465     ASN G   359                                                      
REMARK 465     GLY G   360                                                      
REMARK 465     THR G   361                                                      
REMARK 465     HIS G   362                                                      
REMARK 465     SER G   363                                                      
REMARK 465     TYR G   364                                                      
REMARK 465     ASN G   365                                                      
REMARK 465     GLY G   366                                                      
REMARK 465     THR G   367                                                      
REMARK 465     ASN G   368                                                      
REMARK 465     THR G   369                                                      
REMARK 465     LEU G   370                                                      
REMARK 465     LYS G   371                                                      
REMARK 465     ALA G   372                                                      
REMARK 465     ASP G   373                                                      
REMARK 465     LYS G   374                                                      
REMARK 465     ASN G   375                                                      
REMARK 465     VAL G   376                                                      
REMARK 465     SER G   377                                                      
REMARK 465     LEU G   378                                                      
REMARK 465     SER G   379                                                      
REMARK 465     ILE G   380                                                      
REMARK 465     GLU G   381                                                      
REMARK 465     GLN G   382                                                      
REMARK 465     TYR G   383                                                      
REMARK 465     GLU G   384                                                      
REMARK 465     LYS G   385                                                      
REMARK 465     ILE G   386                                                      
REMARK 465     HIS G   387                                                      
REMARK 465     GLU G   388                                                      
REMARK 465     ALA G   389                                                      
REMARK 465     TYR G   390                                                      
REMARK 465     GLN G   391                                                      
REMARK 465     ILE G   392                                                      
REMARK 465     LEU G   393                                                      
REMARK 465     SER G   394                                                      
REMARK 465     LYS G   395                                                      
REMARK 465     ALA G   396                                                      
REMARK 465     LEU G   397                                                      
REMARK 465     LYS G   398                                                      
REMARK 465     GLN G   399                                                      
REMARK 465     ALA G   400                                                      
REMARK 465     GLY G   401                                                      
REMARK 465     LEU G   402                                                      
REMARK 465     ALA G   403                                                      
REMARK 465     PRO G   404                                                      
REMARK 465     LEU G   405                                                      
REMARK 465     ASN G   406                                                      
REMARK 465     SER G   407                                                      
REMARK 465     LYS G   408                                                      
REMARK 465     GLY G   409                                                      
REMARK 465     GLU G   410                                                      
REMARK 465     LYS G   411                                                      
REMARK 465     LEU G   412                                                      
REMARK 465     GLU G   413                                                      
REMARK 465     ALA G   414                                                      
REMARK 465     HIS G   415                                                      
REMARK 465     VAL G   416                                                      
REMARK 465     THR G   417                                                      
REMARK 465     THR G   418                                                      
REMARK 465     SER G   419                                                      
REMARK 465     LYS G   420                                                      
REMARK 465     TYR G   421                                                      
REMARK 465     HIS G   530                                                      
REMARK 465     HIS G   531                                                      
REMARK 465     HIS G   532                                                      
REMARK 465     HIS G   533                                                      
REMARK 465     HIS G   534                                                      
REMARK 465     HIS G   535                                                      
REMARK 465     GLU G   536                                                      
REMARK 465     PRO G   537                                                      
REMARK 465     GLU G   538                                                      
REMARK 465     ALA G   539                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OG   SER D   276     NZ   LYS E   274              2.11            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A 111       32.80   -140.57                                   
REMARK 500    LEU A 143       31.19    -88.06                                   
REMARK 500    MET A 148       50.05    -91.93                                   
REMARK 500    ARG A 173     -159.40   -146.88                                   
REMARK 500    SER A 276       57.29    -93.70                                   
REMARK 500    LYS A 312       -6.54     71.47                                   
REMARK 500    LYS B 103      117.40   -160.88                                   
REMARK 500    LEU B 140       31.08    -95.63                                   
REMARK 500    LYS C  94      -11.33     73.13                                   
REMARK 500    PRO C 159       43.16   -101.85                                   
REMARK 500    THR C 193       -7.84     73.50                                   
REMARK 500    ARG C 197       51.97    -93.49                                   
REMARK 500    SER C 286       56.52   -103.23                                   
REMARK 500    LYS D 105      -64.34    -93.75                                   
REMARK 500    LEU D 143       36.00    -99.09                                   
REMARK 500    MET D 148       56.19    -96.94                                   
REMARK 500    ARG D 173     -159.71   -147.70                                   
REMARK 500    LEU D 194       51.11    -94.94                                   
REMARK 500    LEU D 277      144.36   -170.04                                   
REMARK 500    LYS D 312       -9.79     74.09                                   
REMARK 500    SER D 390       77.62     60.53                                   
REMARK 500    PRO E 144       40.31   -100.69                                   
REMARK 500    ALA G 497     -168.76   -160.91                                   
REMARK 500    LEU G 510       -7.76     68.55                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PIO A 501                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue RI5 C 501                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PIO D 501                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG A    
REMARK 800  502 through MAN A 510 bound to ASN A 111                            
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG B    
REMARK 800  501 through NAG B 502 bound to ASN B 80                             
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG B    
REMARK 800  503 through MAN B 508 bound to ASN B 149                            
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG C    
REMARK 800  502 through NAG C 503 bound to ASN C 208                            
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG D    
REMARK 800  502 through MAN D 506 bound to ASN D 111                            
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC9                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG E    
REMARK 800  501 through NAG E 502 bound to ASN E 80                             
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Poly-Saccharide residues NAG E    
REMARK 800  503 through MAN E 508 bound to ASN E 149                            
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-0275   RELATED DB: EMDB                              
REMARK 900 CRYOEM STRUCTURE OF HUMAN FULL-LENGTH ALPHA1BETA3GAMMA2L GABA(A)R    
REMARK 900 IN COMPLEX WITH PICROTOXIN AND MEGABODY MB38.                        
DBREF  6HUG A    1   429  UNP    P08219   GBRA1_BOVIN     28    456             
DBREF  6HUG B  -24   448  UNP    P28472   GBRB3_HUMAN      1    473             
DBREF  6HUG C  -38   436  UNP    P18507   GBRG2_HUMAN      1    475             
DBREF  6HUG D    1   429  UNP    P08219   GBRA1_BOVIN     28    456             
DBREF  6HUG E  -24   448  UNP    P28472   GBRB3_HUMAN      1    473             
DBREF  6HUG G    1   539  PDB    6HUG     6HUG             1    539             
SEQADV 6HUG ASP A   -7  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG TYR A   -6  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG LYS A   -5  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP A   -4  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP A   -3  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP A   -2  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP A   -1  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG LYS A    0  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG GLY C  437  UNP  P18507              LINKER                         
SEQADV 6HUG GLY C  438  UNP  P18507              LINKER                         
SEQADV 6HUG SER C  439  UNP  P18507              LINKER                         
SEQADV 6HUG GLY C  440  UNP  P18507              LINKER                         
SEQADV 6HUG GLY C  441  UNP  P18507              LINKER                         
SEQADV 6HUG SER C  442  UNP  P18507              LINKER                         
SEQADV 6HUG GLY C  443  UNP  P18507              LINKER                         
SEQADV 6HUG GLY C  444  UNP  P18507              LINKER                         
SEQADV 6HUG SER C  445  UNP  P18507              LINKER                         
SEQADV 6HUG GLY C  446  UNP  P18507              LINKER                         
SEQADV 6HUG LYS C  447  UNP  P18507              LINKER                         
SEQADV 6HUG THR C  448  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG GLU C  449  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG THR C  450  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG SER C  451  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG GLN C  452  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG VAL C  453  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG ALA C  454  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG PRO C  455  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG ALA C  456  UNP  P18507              EXPRESSION TAG                 
SEQADV 6HUG ASP D   -7  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG TYR D   -6  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG LYS D   -5  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP D   -4  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP D   -3  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP D   -2  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG ASP D   -1  UNP  P08219              EXPRESSION TAG                 
SEQADV 6HUG LYS D    0  UNP  P08219              EXPRESSION TAG                 
SEQRES   1 A  437  ASP TYR LYS ASP ASP ASP ASP LYS GLN PRO SER LEU GLN          
SEQRES   2 A  437  ASP GLU LEU LYS ASP ASN THR THR VAL PHE THR ARG ILE          
SEQRES   3 A  437  LEU ASP ARG LEU LEU ASP GLY TYR ASP ASN ARG LEU ARG          
SEQRES   4 A  437  PRO GLY LEU GLY GLU ARG VAL THR GLU VAL LYS THR ASP          
SEQRES   5 A  437  ILE PHE VAL THR SER PHE GLY PRO VAL SER ASP HIS ASP          
SEQRES   6 A  437  MET GLU TYR THR ILE ASP VAL PHE PHE ARG GLN SER TRP          
SEQRES   7 A  437  LYS ASP GLU ARG LEU LYS PHE LYS GLY PRO MET THR VAL          
SEQRES   8 A  437  LEU ARG LEU ASN ASN LEU MET ALA SER LYS ILE TRP THR          
SEQRES   9 A  437  PRO ASP THR PHE PHE HIS ASN GLY LYS LYS SER VAL ALA          
SEQRES  10 A  437  HIS ASN MET THR MET PRO ASN LYS LEU LEU ARG ILE THR          
SEQRES  11 A  437  GLU ASP GLY THR LEU LEU TYR THR MET ARG LEU THR VAL          
SEQRES  12 A  437  ARG ALA GLU CYS PRO MET HIS LEU GLU ASP PHE PRO MET          
SEQRES  13 A  437  ASP ALA HIS ALA CYS PRO LEU LYS PHE GLY SER TYR ALA          
SEQRES  14 A  437  TYR THR ARG ALA GLU VAL VAL TYR GLU TRP THR ARG GLU          
SEQRES  15 A  437  PRO ALA ARG SER VAL VAL VAL ALA GLU ASP GLY SER ARG          
SEQRES  16 A  437  LEU ASN GLN TYR ASP LEU LEU GLY GLN THR VAL ASP SER          
SEQRES  17 A  437  GLY ILE VAL GLN SER SER THR GLY GLU TYR VAL VAL MET          
SEQRES  18 A  437  THR THR HIS PHE HIS LEU LYS ARG LYS ILE GLY TYR PHE          
SEQRES  19 A  437  VAL ILE GLN THR TYR LEU PRO CYS ILE MET THR VAL ILE          
SEQRES  20 A  437  LEU SER GLN VAL SER PHE TRP LEU ASN ARG GLU SER VAL          
SEQRES  21 A  437  PRO ALA ARG THR VAL PHE GLY VAL THR THR VAL LEU THR          
SEQRES  22 A  437  MET THR THR LEU SER ILE SER ALA ARG ASN SER LEU PRO          
SEQRES  23 A  437  LYS VAL ALA TYR ALA THR ALA MET ASP TRP PHE ILE ALA          
SEQRES  24 A  437  VAL CYS TYR ALA PHE VAL PHE SER ALA LEU ILE GLU PHE          
SEQRES  25 A  437  ALA THR VAL ASN TYR PHE THR LYS ARG GLY TYR ALA TRP          
SEQRES  26 A  437  ASP GLY LYS SER VAL VAL PRO GLU LYS PRO LYS LYS VAL          
SEQRES  27 A  437  LYS ASP PRO LEU ILE LYS LYS ASN ASN THR TYR ALA PRO          
SEQRES  28 A  437  THR ALA THR SER TYR THR PRO ASN LEU ALA ARG GLY ASP          
SEQRES  29 A  437  PRO GLY LEU ALA THR ILE ALA LYS SER ALA THR ILE GLU          
SEQRES  30 A  437  PRO LYS GLU VAL LYS PRO GLU THR LYS PRO PRO GLU PRO          
SEQRES  31 A  437  LYS LYS THR PHE ASN SER VAL SER LYS ILE ASP ARG LEU          
SEQRES  32 A  437  SER ARG ILE ALA PHE PRO LEU LEU PHE GLY ILE PHE ASN          
SEQRES  33 A  437  LEU VAL TYR TRP ALA THR TYR LEU ASN ARG GLU PRO GLN          
SEQRES  34 A  437  LEU LYS ALA PRO THR PRO HIS GLN                              
SEQRES   1 B  473  MET CYS SER GLY LEU LEU GLU LEU LEU LEU PRO ILE TRP          
SEQRES   2 B  473  LEU SER TRP THR LEU GLY THR ARG GLY SER GLU PRO ARG          
SEQRES   3 B  473  SER VAL ASN ASP PRO GLY ASN MET SER PHE VAL LYS GLU          
SEQRES   4 B  473  THR VAL ASP LYS LEU LEU LYS GLY TYR ASP ILE ARG LEU          
SEQRES   5 B  473  ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS VAL GLY MET          
SEQRES   6 B  473  ASN ILE ASP ILE ALA SER ILE ASP MET VAL SER GLU VAL          
SEQRES   7 B  473  ASN MET ASP TYR THR LEU THR MET TYR PHE GLN GLN TYR          
SEQRES   8 B  473  TRP ARG ASP LYS ARG LEU ALA TYR SER GLY ILE PRO LEU          
SEQRES   9 B  473  ASN LEU THR LEU ASP ASN ARG VAL ALA ASP GLN LEU TRP          
SEQRES  10 B  473  VAL PRO ASP THR TYR PHE LEU ASN ASP LYS LYS SER PHE          
SEQRES  11 B  473  VAL HIS GLY VAL THR VAL LYS ASN ARG MET ILE ARG LEU          
SEQRES  12 B  473  HIS PRO ASP GLY THR VAL LEU TYR GLY LEU ARG ILE THR          
SEQRES  13 B  473  THR THR ALA ALA CYS MET MET ASP LEU ARG ARG TYR PRO          
SEQRES  14 B  473  LEU ASP GLU GLN ASN CYS THR LEU GLU ILE GLU SER TYR          
SEQRES  15 B  473  GLY TYR THR THR ASP ASP ILE GLU PHE TYR TRP ARG GLY          
SEQRES  16 B  473  GLY ASP LYS ALA VAL THR GLY VAL GLU ARG ILE GLU LEU          
SEQRES  17 B  473  PRO GLN PHE SER ILE VAL GLU HIS ARG LEU VAL SER ARG          
SEQRES  18 B  473  ASN VAL VAL PHE ALA THR GLY ALA TYR PRO ARG LEU SER          
SEQRES  19 B  473  LEU SER PHE ARG LEU LYS ARG ASN ILE GLY TYR PHE ILE          
SEQRES  20 B  473  LEU GLN THR TYR MET PRO SER ILE LEU ILE THR ILE LEU          
SEQRES  21 B  473  SER TRP VAL SER PHE TRP ILE ASN TYR ASP ALA SER ALA          
SEQRES  22 B  473  ALA ARG VAL ALA LEU GLY ILE THR THR VAL LEU THR MET          
SEQRES  23 B  473  THR THR ILE ASN THR HIS LEU ARG GLU THR LEU PRO LYS          
SEQRES  24 B  473  ILE PRO TYR VAL LYS ALA ILE ASP MET TYR LEU MET GLY          
SEQRES  25 B  473  CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU GLU TYR ALA          
SEQRES  26 B  473  PHE VAL ASN TYR ILE PHE PHE GLY ARG GLY PRO GLN ARG          
SEQRES  27 B  473  GLN LYS LYS LEU ALA GLU LYS THR ALA LYS ALA LYS ASN          
SEQRES  28 B  473  ASP ARG SER LYS SER GLU SER ASN ARG VAL ASP ALA HIS          
SEQRES  29 B  473  GLY ASN ILE LEU LEU THR SER LEU GLU VAL HIS ASN GLU          
SEQRES  30 B  473  MET ASN GLU VAL SER GLY GLY ILE GLY ASP THR ARG ASN          
SEQRES  31 B  473  SER ALA ILE SER PHE ASP ASN SER GLY ILE GLN TYR ARG          
SEQRES  32 B  473  LYS GLN SER MET PRO ARG GLU GLY HIS GLY ARG PHE LEU          
SEQRES  33 B  473  GLY ASP ARG SER LEU PRO HIS LYS LYS THR HIS LEU ARG          
SEQRES  34 B  473  ARG ARG SER SER GLN LEU LYS ILE LYS ILE PRO ASP LEU          
SEQRES  35 B  473  THR ASP VAL ASN ALA ILE ASP ARG TRP SER ARG ILE VAL          
SEQRES  36 B  473  PHE PRO PHE THR PHE SER LEU PHE ASN LEU VAL TYR TRP          
SEQRES  37 B  473  LEU TYR TYR VAL ASN                                          
SEQRES   1 C  495  MET SER SER PRO ASN ILE TRP SER THR GLY SER SER VAL          
SEQRES   2 C  495  TYR SER THR PRO VAL PHE SER GLN LYS MET THR VAL TRP          
SEQRES   3 C  495  ILE LEU LEU LEU LEU SER LEU TYR PRO GLY PHE THR SER          
SEQRES   4 C  495  GLN LYS SER ASP ASP ASP TYR GLU ASP TYR ALA SER ASN          
SEQRES   5 C  495  LYS THR TRP VAL LEU THR PRO LYS VAL PRO GLU GLY ASP          
SEQRES   6 C  495  VAL THR VAL ILE LEU ASN ASN LEU LEU GLU GLY TYR ASP          
SEQRES   7 C  495  ASN LYS LEU ARG PRO ASP ILE GLY VAL LYS PRO THR LEU          
SEQRES   8 C  495  ILE HIS THR ASP MET TYR VAL ASN SER ILE GLY PRO VAL          
SEQRES   9 C  495  ASN ALA ILE ASN MET GLU TYR THR ILE ASP ILE PHE PHE          
SEQRES  10 C  495  ALA GLN THR TRP TYR ASP ARG ARG LEU LYS PHE ASN SER          
SEQRES  11 C  495  THR ILE LYS VAL LEU ARG LEU ASN SER ASN MET VAL GLY          
SEQRES  12 C  495  LYS ILE TRP ILE PRO ASP THR PHE PHE ARG ASN SER LYS          
SEQRES  13 C  495  LYS ALA ASP ALA HIS TRP ILE THR THR PRO ASN ARG MET          
SEQRES  14 C  495  LEU ARG ILE TRP ASN ASP GLY ARG VAL LEU TYR THR LEU          
SEQRES  15 C  495  ARG LEU THR ILE ASP ALA GLU CYS GLN LEU GLN LEU HIS          
SEQRES  16 C  495  ASN PHE PRO MET ASP GLU HIS SER CYS PRO LEU GLU PHE          
SEQRES  17 C  495  SER SER TYR GLY TYR PRO ARG GLU GLU ILE VAL TYR GLN          
SEQRES  18 C  495  TRP LYS ARG SER SER VAL GLU VAL GLY ASP THR ARG SER          
SEQRES  19 C  495  TRP ARG LEU TYR GLN PHE SER PHE VAL GLY LEU ARG ASN          
SEQRES  20 C  495  THR THR GLU VAL VAL LYS THR THR SER GLY ASP TYR VAL          
SEQRES  21 C  495  VAL MET SER VAL TYR PHE ASP LEU SER ARG ARG MET GLY          
SEQRES  22 C  495  TYR PHE THR ILE GLN THR TYR ILE PRO CYS THR LEU ILE          
SEQRES  23 C  495  VAL VAL LEU SER TRP VAL SER PHE TRP ILE ASN LYS ASP          
SEQRES  24 C  495  ALA VAL PRO ALA ARG THR SER LEU GLY ILE THR THR VAL          
SEQRES  25 C  495  LEU THR MET THR THR LEU SER THR ILE ALA ARG LYS SER          
SEQRES  26 C  495  LEU PRO LYS VAL SER TYR VAL THR ALA MET ASP LEU PHE          
SEQRES  27 C  495  VAL SER VAL CYS PHE ILE PHE VAL PHE SER ALA LEU VAL          
SEQRES  28 C  495  GLU TYR GLY THR LEU HIS TYR PHE VAL SER ASN ARG LYS          
SEQRES  29 C  495  PRO SER LYS ASP LYS ASP LYS LYS LYS LYS ASN PRO LEU          
SEQRES  30 C  495  LEU ARG MET PHE SER PHE LYS ALA PRO THR ILE ASP ILE          
SEQRES  31 C  495  ARG PRO ARG SER ALA THR ILE GLN MET ASN ASN ALA THR          
SEQRES  32 C  495  HIS LEU GLN GLU ARG ASP GLU GLU TYR GLY TYR GLU CYS          
SEQRES  33 C  495  LEU ASP GLY LYS ASP CYS ALA SER PHE PHE CYS CYS PHE          
SEQRES  34 C  495  GLU ASP CYS ARG THR GLY ALA TRP ARG HIS GLY ARG ILE          
SEQRES  35 C  495  HIS ILE ARG ILE ALA LYS MET ASP SER TYR ALA ARG ILE          
SEQRES  36 C  495  PHE PHE PRO THR ALA PHE CYS LEU PHE ASN LEU VAL TYR          
SEQRES  37 C  495  TRP VAL SER TYR LEU TYR LEU GLY GLY SER GLY GLY SER          
SEQRES  38 C  495  GLY GLY SER GLY LYS THR GLU THR SER GLN VAL ALA PRO          
SEQRES  39 C  495  ALA                                                          
SEQRES   1 D  437  ASP TYR LYS ASP ASP ASP ASP LYS GLN PRO SER LEU GLN          
SEQRES   2 D  437  ASP GLU LEU LYS ASP ASN THR THR VAL PHE THR ARG ILE          
SEQRES   3 D  437  LEU ASP ARG LEU LEU ASP GLY TYR ASP ASN ARG LEU ARG          
SEQRES   4 D  437  PRO GLY LEU GLY GLU ARG VAL THR GLU VAL LYS THR ASP          
SEQRES   5 D  437  ILE PHE VAL THR SER PHE GLY PRO VAL SER ASP HIS ASP          
SEQRES   6 D  437  MET GLU TYR THR ILE ASP VAL PHE PHE ARG GLN SER TRP          
SEQRES   7 D  437  LYS ASP GLU ARG LEU LYS PHE LYS GLY PRO MET THR VAL          
SEQRES   8 D  437  LEU ARG LEU ASN ASN LEU MET ALA SER LYS ILE TRP THR          
SEQRES   9 D  437  PRO ASP THR PHE PHE HIS ASN GLY LYS LYS SER VAL ALA          
SEQRES  10 D  437  HIS ASN MET THR MET PRO ASN LYS LEU LEU ARG ILE THR          
SEQRES  11 D  437  GLU ASP GLY THR LEU LEU TYR THR MET ARG LEU THR VAL          
SEQRES  12 D  437  ARG ALA GLU CYS PRO MET HIS LEU GLU ASP PHE PRO MET          
SEQRES  13 D  437  ASP ALA HIS ALA CYS PRO LEU LYS PHE GLY SER TYR ALA          
SEQRES  14 D  437  TYR THR ARG ALA GLU VAL VAL TYR GLU TRP THR ARG GLU          
SEQRES  15 D  437  PRO ALA ARG SER VAL VAL VAL ALA GLU ASP GLY SER ARG          
SEQRES  16 D  437  LEU ASN GLN TYR ASP LEU LEU GLY GLN THR VAL ASP SER          
SEQRES  17 D  437  GLY ILE VAL GLN SER SER THR GLY GLU TYR VAL VAL MET          
SEQRES  18 D  437  THR THR HIS PHE HIS LEU LYS ARG LYS ILE GLY TYR PHE          
SEQRES  19 D  437  VAL ILE GLN THR TYR LEU PRO CYS ILE MET THR VAL ILE          
SEQRES  20 D  437  LEU SER GLN VAL SER PHE TRP LEU ASN ARG GLU SER VAL          
SEQRES  21 D  437  PRO ALA ARG THR VAL PHE GLY VAL THR THR VAL LEU THR          
SEQRES  22 D  437  MET THR THR LEU SER ILE SER ALA ARG ASN SER LEU PRO          
SEQRES  23 D  437  LYS VAL ALA TYR ALA THR ALA MET ASP TRP PHE ILE ALA          
SEQRES  24 D  437  VAL CYS TYR ALA PHE VAL PHE SER ALA LEU ILE GLU PHE          
SEQRES  25 D  437  ALA THR VAL ASN TYR PHE THR LYS ARG GLY TYR ALA TRP          
SEQRES  26 D  437  ASP GLY LYS SER VAL VAL PRO GLU LYS PRO LYS LYS VAL          
SEQRES  27 D  437  LYS ASP PRO LEU ILE LYS LYS ASN ASN THR TYR ALA PRO          
SEQRES  28 D  437  THR ALA THR SER TYR THR PRO ASN LEU ALA ARG GLY ASP          
SEQRES  29 D  437  PRO GLY LEU ALA THR ILE ALA LYS SER ALA THR ILE GLU          
SEQRES  30 D  437  PRO LYS GLU VAL LYS PRO GLU THR LYS PRO PRO GLU PRO          
SEQRES  31 D  437  LYS LYS THR PHE ASN SER VAL SER LYS ILE ASP ARG LEU          
SEQRES  32 D  437  SER ARG ILE ALA PHE PRO LEU LEU PHE GLY ILE PHE ASN          
SEQRES  33 D  437  LEU VAL TYR TRP ALA THR TYR LEU ASN ARG GLU PRO GLN          
SEQRES  34 D  437  LEU LYS ALA PRO THR PRO HIS GLN                              
SEQRES   1 E  473  MET CYS SER GLY LEU LEU GLU LEU LEU LEU PRO ILE TRP          
SEQRES   2 E  473  LEU SER TRP THR LEU GLY THR ARG GLY SER GLU PRO ARG          
SEQRES   3 E  473  SER VAL ASN ASP PRO GLY ASN MET SER PHE VAL LYS GLU          
SEQRES   4 E  473  THR VAL ASP LYS LEU LEU LYS GLY TYR ASP ILE ARG LEU          
SEQRES   5 E  473  ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS VAL GLY MET          
SEQRES   6 E  473  ASN ILE ASP ILE ALA SER ILE ASP MET VAL SER GLU VAL          
SEQRES   7 E  473  ASN MET ASP TYR THR LEU THR MET TYR PHE GLN GLN TYR          
SEQRES   8 E  473  TRP ARG ASP LYS ARG LEU ALA TYR SER GLY ILE PRO LEU          
SEQRES   9 E  473  ASN LEU THR LEU ASP ASN ARG VAL ALA ASP GLN LEU TRP          
SEQRES  10 E  473  VAL PRO ASP THR TYR PHE LEU ASN ASP LYS LYS SER PHE          
SEQRES  11 E  473  VAL HIS GLY VAL THR VAL LYS ASN ARG MET ILE ARG LEU          
SEQRES  12 E  473  HIS PRO ASP GLY THR VAL LEU TYR GLY LEU ARG ILE THR          
SEQRES  13 E  473  THR THR ALA ALA CYS MET MET ASP LEU ARG ARG TYR PRO          
SEQRES  14 E  473  LEU ASP GLU GLN ASN CYS THR LEU GLU ILE GLU SER TYR          
SEQRES  15 E  473  GLY TYR THR THR ASP ASP ILE GLU PHE TYR TRP ARG GLY          
SEQRES  16 E  473  GLY ASP LYS ALA VAL THR GLY VAL GLU ARG ILE GLU LEU          
SEQRES  17 E  473  PRO GLN PHE SER ILE VAL GLU HIS ARG LEU VAL SER ARG          
SEQRES  18 E  473  ASN VAL VAL PHE ALA THR GLY ALA TYR PRO ARG LEU SER          
SEQRES  19 E  473  LEU SER PHE ARG LEU LYS ARG ASN ILE GLY TYR PHE ILE          
SEQRES  20 E  473  LEU GLN THR TYR MET PRO SER ILE LEU ILE THR ILE LEU          
SEQRES  21 E  473  SER TRP VAL SER PHE TRP ILE ASN TYR ASP ALA SER ALA          
SEQRES  22 E  473  ALA ARG VAL ALA LEU GLY ILE THR THR VAL LEU THR MET          
SEQRES  23 E  473  THR THR ILE ASN THR HIS LEU ARG GLU THR LEU PRO LYS          
SEQRES  24 E  473  ILE PRO TYR VAL LYS ALA ILE ASP MET TYR LEU MET GLY          
SEQRES  25 E  473  CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU GLU TYR ALA          
SEQRES  26 E  473  PHE VAL ASN TYR ILE PHE PHE GLY ARG GLY PRO GLN ARG          
SEQRES  27 E  473  GLN LYS LYS LEU ALA GLU LYS THR ALA LYS ALA LYS ASN          
SEQRES  28 E  473  ASP ARG SER LYS SER GLU SER ASN ARG VAL ASP ALA HIS          
SEQRES  29 E  473  GLY ASN ILE LEU LEU THR SER LEU GLU VAL HIS ASN GLU          
SEQRES  30 E  473  MET ASN GLU VAL SER GLY GLY ILE GLY ASP THR ARG ASN          
SEQRES  31 E  473  SER ALA ILE SER PHE ASP ASN SER GLY ILE GLN TYR ARG          
SEQRES  32 E  473  LYS GLN SER MET PRO ARG GLU GLY HIS GLY ARG PHE LEU          
SEQRES  33 E  473  GLY ASP ARG SER LEU PRO HIS LYS LYS THR HIS LEU ARG          
SEQRES  34 E  473  ARG ARG SER SER GLN LEU LYS ILE LYS ILE PRO ASP LEU          
SEQRES  35 E  473  THR ASP VAL ASN ALA ILE ASP ARG TRP SER ARG ILE VAL          
SEQRES  36 E  473  PHE PRO PHE THR PHE SER LEU PHE ASN LEU VAL TYR TRP          
SEQRES  37 E  473  LEU TYR TYR VAL ASN                                          
SEQRES   1 G  539  GLN VAL GLN LEU GLN GLU SER GLY GLY GLY LEU VAL GLN          
SEQRES   2 G  539  THR LYS THR THR THR SER VAL ILE ASP THR THR ASN ASP          
SEQRES   3 G  539  ALA GLN ASN LEU LEU THR GLN ALA GLN THR ILE VAL ASN          
SEQRES   4 G  539  THR LEU LYS ASP TYR CYS PRO ILE LEU ILE ALA LYS SER          
SEQRES   5 G  539  SER SER SER ASN GLY GLY THR ASN ASN ALA ASN THR PRO          
SEQRES   6 G  539  SER TRP GLN THR ALA GLY GLY GLY LYS ASN SER CYS ALA          
SEQRES   7 G  539  THR PHE GLY ALA GLU PHE SER ALA ALA SER ASP MET ILE          
SEQRES   8 G  539  ASN ASN ALA GLN LYS ILE VAL GLN GLU THR GLN GLN LEU          
SEQRES   9 G  539  SER ALA ASN GLN PRO LYS ASN ILE THR GLN PRO HIS ASN          
SEQRES  10 G  539  LEU ASN LEU ASN SER PRO SER SER LEU THR ALA LEU ALA          
SEQRES  11 G  539  GLN LYS MET LEU LYS ASN ALA GLN SER GLN ALA GLU ILE          
SEQRES  12 G  539  LEU LYS LEU ALA ASN GLN VAL GLU SER ASP PHE ASN LYS          
SEQRES  13 G  539  LEU SER SER GLY HIS LEU LYS ASP TYR ILE GLY LYS CYS          
SEQRES  14 G  539  ASP ALA SER ALA ILE SER SER ALA ASN MET THR MET GLN          
SEQRES  15 G  539  ASN GLN LYS ASN ASN TRP GLY ASN GLY CYS ALA GLY VAL          
SEQRES  16 G  539  GLU GLU THR GLN SER LEU LEU LYS THR SER ALA ALA ASP          
SEQRES  17 G  539  PHE ASN ASN GLN THR PRO GLN ILE ASN GLN ALA GLN ASN          
SEQRES  18 G  539  LEU ALA ASN THR LEU ILE GLN GLU LEU GLY ASN ASN PRO          
SEQRES  19 G  539  PHE ARG ALA SER GLY GLY GLY SER GLY GLY GLY GLY SER          
SEQRES  20 G  539  GLY LYS LEU SER ASP THR TYR GLU GLN LEU SER ARG LEU          
SEQRES  21 G  539  LEU THR ASN ASP ASN GLY THR ASN SER LYS THR SER ALA          
SEQRES  22 G  539  GLN ALA ILE ASN GLN ALA VAL ASN ASN LEU ASN GLU ARG          
SEQRES  23 G  539  ALA LYS THR LEU ALA GLY GLY THR THR ASN SER PRO ALA          
SEQRES  24 G  539  TYR GLN ALA THR LEU LEU ALA LEU ARG SER VAL LEU GLY          
SEQRES  25 G  539  LEU TRP ASN SER MET GLY TYR ALA VAL ILE CYS GLY GLY          
SEQRES  26 G  539  TYR THR LYS SER PRO GLY GLU ASN ASN GLN LYS ASP PHE          
SEQRES  27 G  539  HIS TYR THR ASP GLU ASN GLY ASN GLY THR THR ILE ASN          
SEQRES  28 G  539  CYS GLY GLY SER THR ASN SER ASN GLY THR HIS SER TYR          
SEQRES  29 G  539  ASN GLY THR ASN THR LEU LYS ALA ASP LYS ASN VAL SER          
SEQRES  30 G  539  LEU SER ILE GLU GLN TYR GLU LYS ILE HIS GLU ALA TYR          
SEQRES  31 G  539  GLN ILE LEU SER LYS ALA LEU LYS GLN ALA GLY LEU ALA          
SEQRES  32 G  539  PRO LEU ASN SER LYS GLY GLU LYS LEU GLU ALA HIS VAL          
SEQRES  33 G  539  THR THR SER LYS TYR GLY SER LEU ARG VAL SER CYS ALA          
SEQRES  34 G  539  ALA SER GLY ARG THR PHE THR THR TYR ILE MET ALA TRP          
SEQRES  35 G  539  PHE ARG GLN ALA PRO GLY LYS GLU ARG GLU PHE LEU ALA          
SEQRES  36 G  539  ALA MET ASP GLN GLY ARG ILE GLN TYR TYR GLY ASP SER          
SEQRES  37 G  539  VAL ARG GLY ARG PHE THR ILE SER ARG ASP TYR ALA LYS          
SEQRES  38 G  539  ASN SER VAL ASP LEU GLN LEU ASP GLY LEU ARG PRO GLU          
SEQRES  39 G  539  ASP THR ALA VAL TYR TYR CYS ALA ALA GLY ALA GLY PHE          
SEQRES  40 G  539  TRP GLY LEU ARG THR ALA SER SER TYR HIS TYR TRP GLY          
SEQRES  41 G  539  GLN GLY THR GLN VAL THR VAL SER SER HIS HIS HIS HIS          
SEQRES  42 G  539  HIS HIS GLU PRO GLU ALA                                      
HET    PIO  A 501      47                                                       
HET    NAG  A 502      14                                                       
HET    NAG  A 503      14                                                       
HET    BMA  A 504      11                                                       
HET    MAN  A 505      11                                                       
HET    MAN  A 506      11                                                       
HET    MAN  A 507      11                                                       
HET    MAN  A 508      11                                                       
HET    MAN  A 509      11                                                       
HET    MAN  A 510      11                                                       
HET    NAG  B 501      14                                                       
HET    NAG  B 502      14                                                       
HET    NAG  B 503      14                                                       
HET    NAG  B 504      14                                                       
HET    BMA  B 505      11                                                       
HET    MAN  B 506      11                                                       
HET    MAN  B 507      11                                                       
HET    MAN  B 508      11                                                       
HET    RI5  C 501      21                                                       
HET    NAG  C 502      14                                                       
HET    NAG  C 503      14                                                       
HET    PIO  D 501      47                                                       
HET    NAG  D 502      14                                                       
HET    NAG  D 503      14                                                       
HET    BMA  D 504      11                                                       
HET    MAN  D 505      11                                                       
HET    MAN  D 506      11                                                       
HET    NAG  E 501      14                                                       
HET    NAG  E 502      14                                                       
HET    NAG  E 503      14                                                       
HET    NAG  E 504      14                                                       
HET    BMA  E 505      11                                                       
HET    MAN  E 506      11                                                       
HET    MAN  E 507      11                                                       
HET    MAN  E 508      11                                                       
HETNAM     PIO [(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,          
HETNAM   2 PIO  3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-           
HETNAM   3 PIO  PHOSPHORYL]OXY-PROPYL] OCTANOATE                                
HETNAM     NAG N-ACETYL-D-GLUCOSAMINE                                           
HETNAM     BMA BETA-D-MANNOSE                                                   
HETNAM     MAN ALPHA-D-MANNOSE                                                  
HETNAM     RI5 (1AR,2AR,3S,6R,6AS,8AS,8BR,9R)-2A-HYDROXY-8B-METHYL-9-           
HETNAM   2 RI5  (PROP-1-EN-2-YL)HEXAHYDRO-3,6-METHANO-1,5,7-                    
HETNAM   3 RI5  TRIOXACYCLOPENTA[IJ]CYCLOPROPA[A]AZULENE-4,8(3H)-DIONE          
HETSYN     PIO DIOCTANOYL L-ALPHA-PHOSPHATIDYL-D-MYO-INOSITOL 4,5-              
HETSYN   2 PIO  DIPHOSPHATE                                                     
HETSYN     RI5 PICROTOXIN                                                       
FORMUL   7  PIO    2(C25 H49 O19 P3)                                            
FORMUL   8  NAG    14(C8 H15 N O6)                                              
FORMUL   8  BMA    4(C6 H12 O6)                                                 
FORMUL   8  MAN    14(C6 H12 O6)                                                
FORMUL  11  RI5    C15 H16 O6                                                   
HELIX    1 AA1 ASP A   10  ARG A   21  1                                  12    
HELIX    2 AA2 GLU A   73  LYS A   76  5                                   4    
HELIX    3 AA3 ASN A   87  SER A   92  1                                   6    
HELIX    4 AA4 HIS A  142  PHE A  146  5                                   5    
HELIX    5 AA5 ILE A  223  THR A  230  1                                   8    
HELIX    6 AA6 THR A  230  SER A  244  1                                  15    
HELIX    7 AA7 PHE A  245  LEU A  247  5                                   3    
HELIX    8 AA8 SER A  251  SER A  276  1                                  26    
HELIX    9 AA9 THR A  284  PHE A  310  1                                  27    
HELIX   10 AB1 SER A  390  TYR A  415  1                                  26    
HELIX   11 AB2 MET B    9  LYS B   21  1                                  13    
HELIX   12 AB3 LYS B   70  ALA B   73  5                                   4    
HELIX   13 AB4 ASP B   84  ALA B   88  5                                   5    
HELIX   14 AB5 ASP B  139  TYR B  143  5                                   5    
HELIX   15 AB6 ILE B  218  THR B  225  1                                   8    
HELIX   16 AB7 THR B  225  SER B  236  1                                  12    
HELIX   17 AB8 TRP B  237  ILE B  242  5                                   6    
HELIX   18 AB9 ALA B  246  LEU B  272  1                                  27    
HELIX   19 AC1 LYS B  279  GLY B  308  1                                  30    
HELIX   20 AC2 ASP B  419  TYR B  446  1                                  28    
HELIX   21 AC3 VAL C   27  LEU C   35  1                                   9    
HELIX   22 AC4 ARG C   85  LYS C   88  5                                   4    
HELIX   23 AC5 THR C  240  VAL C  253  1                                  14    
HELIX   24 AC6 SER C  254  ILE C  257  5                                   4    
HELIX   25 AC7 ALA C  261  THR C  281  1                                  21    
HELIX   26 AC8 ILE C  282  LYS C  285  5                                   4    
HELIX   27 AC9 THR C  294  SER C  322  1                                  29    
HELIX   28 AD1 LYS C  409  TYR C  435  1                                  27    
HELIX   29 AD2 ASP D   10  ARG D   21  1                                  12    
HELIX   30 AD3 GLU D   73  LYS D   76  5                                   4    
HELIX   31 AD4 ASN D   87  SER D   92  1                                   6    
HELIX   32 AD5 GLU D  174  VAL D  179  1                                   6    
HELIX   33 AD6 ILE D  223  THR D  230  1                                   8    
HELIX   34 AD7 THR D  230  VAL D  243  1                                  14    
HELIX   35 AD8 SER D  244  LEU D  247  5                                   4    
HELIX   36 AD9 SER D  251  SER D  276  1                                  26    
HELIX   37 AE1 THR D  284  TYR D  309  1                                  26    
HELIX   38 AE2 SER D  390  TYR D  415  1                                  26    
HELIX   39 AE3 MET E    9  LEU E   20  1                                  12    
HELIX   40 AE4 LYS E   70  ALA E   73  5                                   4    
HELIX   41 AE5 ASP E   84  LEU E   91  5                                   8    
HELIX   42 AE6 GLY E  170  ALA E  174  5                                   5    
HELIX   43 AE7 ILE E  218  THR E  225  1                                   8    
HELIX   44 AE8 THR E  225  TRP E  237  1                                  13    
HELIX   45 AE9 VAL E  238  TRP E  241  5                                   4    
HELIX   46 AF1 ALA E  246  LEU E  272  1                                  27    
HELIX   47 AF2 LYS E  279  GLY E  308  1                                  30    
HELIX   48 AF3 VAL E  420  TYR E  446  1                                  27    
HELIX   49 AF4 ARG G  492  THR G  496  5                                   5    
HELIX   50 AF5 THR G  512  TYR G  516  5                                   5    
SHEET    1 AA1 6 VAL A  83  ARG A  85  0                                        
SHEET    2 AA1 6 LYS A 117  THR A 122 -1  O  ILE A 121   N  LEU A  84           
SHEET    3 AA1 6 THR A 126  GLU A 138 -1  O  LEU A 128   N  ARG A 120           
SHEET    4 AA1 6 GLU A  59  LYS A  71 -1  N  GLN A  68   O  TYR A 129           
SHEET    5 AA1 6 THR A  39  PHE A  50 -1  N  ASP A  44   O  ARG A  67           
SHEET    6 AA1 6 VAL A 167  TRP A 171  1  O  GLU A 170   N  THR A  43           
SHEET    1 AA2 5 VAL A 108  ALA A 109  0                                        
SHEET    2 AA2 5 THR A 126  GLU A 138 -1  O  THR A 134   N  VAL A 108           
SHEET    3 AA2 5 GLU A  59  LYS A  71 -1  N  GLN A  68   O  TYR A 129           
SHEET    4 AA2 5 THR A  39  PHE A  50 -1  N  ASP A  44   O  ARG A  67           
SHEET    5 AA2 5 VAL A 179  VAL A 181  1  O  VAL A 180   N  VAL A  47           
SHEET    1 AA310 THR A  99  PHE A 101  0                                        
SHEET    2 AA310 ALA A 150  SER A 159 -1  O  GLY A 158   N  PHE A 100           
SHEET    3 AA310 GLU A 209  ARG A 221 -1  O  PHE A 217   N  CYS A 153           
SHEET    4 AA310 TYR A 191  GLN A 204 -1  N  GLY A 201   O  VAL A 212           
SHEET    5 AA310 ILE G 462  TYR G 465 -1  O  GLN G 463   N  ILE A 202           
SHEET    6 AA310 ARG G 451  MET G 457 -1  N  ALA G 456   O  TYR G 464           
SHEET    7 AA310 ILE G 439  GLN G 445 -1  N  ARG G 444   O  GLU G 452           
SHEET    8 AA310 ALA G 497  GLY G 504 -1  O  GLY G 504   N  ILE G 439           
SHEET    9 AA310 GLN G 524  VAL G 527 -1  O  VAL G 525   N  ALA G 497           
SHEET   10 AA310 LEU G  11  VAL G  12  1  N  VAL G  12   O  THR G 526           
SHEET    1 AA4 4 LEU B  81  LEU B  83  0                                        
SHEET    2 AA4 4 MET B 115  LEU B 118 -1  O  ILE B 116   N  LEU B  83           
SHEET    3 AA4 4 THR B 123  ALA B 135 -1  O  LEU B 125   N  ARG B 117           
SHEET    4 AA4 4 ASP B 101  VAL B 106 -1  N  LYS B 102   O  THR B 133           
SHEET    1 AA5 6 LEU B  81  LEU B  83  0                                        
SHEET    2 AA5 6 MET B 115  LEU B 118 -1  O  ILE B 116   N  LEU B  83           
SHEET    3 AA5 6 THR B 123  ALA B 135 -1  O  LEU B 125   N  ARG B 117           
SHEET    4 AA5 6 ASP B  56  ARG B  68 -1  N  LEU B  59   O  THR B 132           
SHEET    5 AA5 6 VAL B  36  SER B  51 -1  N  ASP B  43   O  TYR B  62           
SHEET    6 AA5 6 ILE B 164  TRP B 168  1  O  GLU B 165   N  VAL B  38           
SHEET    1 AA6 4 THR B  96  PHE B  98  0                                        
SHEET    2 AA6 4 GLU B 147  SER B 156 -1  O  GLU B 155   N  TYR B  97           
SHEET    3 AA6 4 ALA B 204  ARG B 216 -1  O  LEU B 208   N  ILE B 154           
SHEET    4 AA6 4 PHE B 186  VAL B 199 -1  N  VAL B 189   O  ARG B 213           
SHEET    1 AA7 4 VAL C  95  LEU C  98  0                                        
SHEET    2 AA7 4 ARG C 129  TRP C 134 -1  O  LEU C 131   N  LEU C  98           
SHEET    3 AA7 4 ARG C 138  GLU C 150 -1  O  LEU C 140   N  ARG C 132           
SHEET    4 AA7 4 SER C 116  ALA C 121 -1  N  LYS C 118   O  ASP C 148           
SHEET    1 AA8 6 VAL C  95  LEU C  98  0                                        
SHEET    2 AA8 6 ARG C 129  TRP C 134 -1  O  LEU C 131   N  LEU C  98           
SHEET    3 AA8 6 ARG C 138  GLU C 150 -1  O  LEU C 140   N  ARG C 132           
SHEET    4 AA8 6 GLU C  71  TYR C  83 -1  N  ILE C  74   O  ILE C 147           
SHEET    5 AA8 6 THR C  51  ILE C  62 -1  N  ASN C  60   O  ASP C  75           
SHEET    6 AA8 6 ILE C 179  VAL C 190  1  O  GLU C 189   N  MET C  57           
SHEET    1 AA9 4 THR C 111  PHE C 113  0                                        
SHEET    2 AA9 4 GLU C 162  SER C 171 -1  O  SER C 170   N  PHE C 112           
SHEET    3 AA9 4 ASP C 219  ARG C 231 -1  O  LEU C 229   N  HIS C 163           
SHEET    4 AA9 4 PHE C 201  LYS C 214 -1  N  ARG C 207   O  TYR C 226           
SHEET    1 AB1 6 VAL D  83  ARG D  85  0                                        
SHEET    2 AB1 6 LYS D 117  THR D 122 -1  O  ILE D 121   N  LEU D  84           
SHEET    3 AB1 6 THR D 126  GLU D 138 -1  O  LEU D 128   N  ARG D 120           
SHEET    4 AB1 6 GLU D  59  LYS D  71 -1  N  ILE D  62   O  VAL D 135           
SHEET    5 AB1 6 THR D  39  SER D  54 -1  N  LYS D  42   O  SER D  69           
SHEET    6 AB1 6 VAL D 167  TRP D 171  1  O  GLU D 170   N  THR D  43           
SHEET    1 AB2 5 VAL D 108  ALA D 109  0                                        
SHEET    2 AB2 5 THR D 126  GLU D 138 -1  O  THR D 134   N  VAL D 108           
SHEET    3 AB2 5 GLU D  59  LYS D  71 -1  N  ILE D  62   O  VAL D 135           
SHEET    4 AB2 5 THR D  39  SER D  54 -1  N  LYS D  42   O  SER D  69           
SHEET    5 AB2 5 VAL D 180  VAL D 181  1  O  VAL D 180   N  VAL D  47           
SHEET    1 AB3 4 THR D  99  PHE D 101  0                                        
SHEET    2 AB3 4 ALA D 150  SER D 159 -1  O  GLY D 158   N  PHE D 100           
SHEET    3 AB3 4 TYR D 210  ARG D 221 -1  O  MET D 213   N  PHE D 157           
SHEET    4 AB3 4 TYR D 191  VAL D 203 -1  N  GLY D 201   O  VAL D 212           
SHEET    1 AB4 4 LEU E  81  LEU E  83  0                                        
SHEET    2 AB4 4 ARG E 114  LEU E 118 -1  O  ILE E 116   N  LEU E  83           
SHEET    3 AB4 4 THR E 123  ALA E 135 -1  O  LEU E 125   N  ARG E 117           
SHEET    4 AB4 4 ASP E 101  VAL E 106 -1  N  LYS E 102   O  THR E 133           
SHEET    1 AB5 6 LEU E  81  LEU E  83  0                                        
SHEET    2 AB5 6 ARG E 114  LEU E 118 -1  O  ILE E 116   N  LEU E  83           
SHEET    3 AB5 6 THR E 123  ALA E 135 -1  O  LEU E 125   N  ARG E 117           
SHEET    4 AB5 6 ASP E  56  ARG E  68 -1  N  LEU E  59   O  THR E 132           
SHEET    5 AB5 6 VAL E  36  SER E  51 -1  N  GLY E  39   O  TYR E  66           
SHEET    6 AB5 6 ILE E 164  TRP E 168  1  O  GLU E 165   N  VAL E  38           
SHEET    1 AB6 4 THR E  96  PHE E  98  0                                        
SHEET    2 AB6 4 GLU E 147  SER E 156 -1  O  GLU E 155   N  TYR E  97           
SHEET    3 AB6 4 ALA E 204  ARG E 216 -1  O  LEU E 208   N  ILE E 154           
SHEET    4 AB6 4 PHE E 186  VAL E 199 -1  N  VAL E 198   O  TYR E 205           
SHEET    1 AB7 4 GLN G   5  GLU G   6  0                                        
SHEET    2 AB7 4 LEU G 424  ALA G 429 -1  O  ALA G 429   N  GLN G   5           
SHEET    3 AB7 4 SER G 483  LEU G 488 -1  O  LEU G 488   N  LEU G 424           
SHEET    4 AB7 4 THR G 474  ASP G 478 -1  N  THR G 474   O  GLN G 487           
SSBOND   1 CYS A  139    CYS A  153                          1555   1555  2.03  
SSBOND   2 CYS B  136    CYS B  150                          1555   1555  2.04  
SSBOND   3 CYS C  151    CYS C  165                          1555   1555  2.04  
SSBOND   4 CYS D  139    CYS D  153                          1555   1555  2.03  
SSBOND   5 CYS E  136    CYS E  150                          1555   1555  2.03  
SSBOND   6 CYS G  428    CYS G  501                          1555   1555  2.03  
LINK         ND2 ASN A 111                 C1  NAG A 502     1555   1555  1.44  
LINK         ND2 ASN B  80                 C1  NAG B 501     1555   1555  1.44  
LINK         ND2 ASN B 149                 C1  NAG B 503     1555   1555  1.44  
LINK         ND2 ASN C 208                 C1  NAG C 502     1555   1555  1.44  
LINK         ND2 ASN D 111                 C1  NAG D 502     1555   1555  1.51  
LINK         ND2 ASN E  80                 C1  NAG E 501     1555   1555  1.44  
LINK         ND2 ASN E 149                 C1  NAG E 503     1555   1555  1.51  
LINK         O4  NAG A 502                 C1  NAG A 503     1555   1555  1.38  
LINK         O4  NAG A 503                 C1  BMA A 504     1555   1555  1.38  
LINK         O3  BMA A 504                 C1  MAN A 508     1555   1555  1.24  
LINK         O6  BMA A 504                 C1  MAN A 505     1555   1555  1.38  
LINK         O3  MAN A 505                 C1  MAN A 507     1555   1555  1.38  
LINK         O6  MAN A 505                 C1  MAN A 506     1555   1555  1.38  
LINK         O2  MAN A 508                 C1  MAN A 509     1555   1555  1.44  
LINK         O2  MAN A 509                 C1  MAN A 510     1555   1555  1.44  
LINK         O4  NAG B 501                 C1  NAG B 502     1555   1555  1.38  
LINK         O4  NAG B 503                 C1  NAG B 504     1555   1555  1.38  
LINK         O4  NAG B 504                 C1  BMA B 505     1555   1555  1.38  
LINK         O3  BMA B 505                 C1  MAN B 507     1555   1555  1.38  
LINK         O6  BMA B 505                 C1  MAN B 506     1555   1555  1.38  
LINK         O3  MAN B 506                 C1  MAN B 508     1555   1555  1.38  
LINK         O4  NAG C 502                 C1  NAG C 503     1555   1555  1.38  
LINK         O4  NAG D 502                 C1  NAG D 503     1555   1555  1.44  
LINK         O4  NAG D 503                 C1  BMA D 504     1555   1555  1.44  
LINK         O3  BMA D 504                 C1  MAN D 506     1555   1555  1.38  
LINK         O6  BMA D 504                 C1  MAN D 505     1555   1555  1.38  
LINK         O4  NAG E 501                 C1  NAG E 502     1555   1555  1.38  
LINK         O4  NAG E 503                 C1  NAG E 504     1555   1555  1.44  
LINK         O4  NAG E 504                 C1  BMA E 505     1555   1555  1.44  
LINK         O3  BMA E 505                 C1  MAN E 507     1555   1555  1.19  
LINK         O6  BMA E 505                 C1  MAN E 506     1555   1555  1.44  
LINK         O3  MAN E 506                 C1  MAN E 508     1555   1555  1.38  
CISPEP   1 MET A  112    THR A  113          0        -0.66                     
CISPEP   2 PHE A  146    PRO A  147          0         3.30                     
CISPEP   3 VAL B  109    THR B  110          0         2.58                     
CISPEP   4 TYR B  143    PRO B  144          0         2.39                     
CISPEP   5 ILE C  124    THR C  125          0         6.30                     
CISPEP   6 PHE C  158    PRO C  159          0         1.63                     
CISPEP   7 MET D  112    THR D  113          0        -2.46                     
CISPEP   8 PHE D  146    PRO D  147          0        -0.74                     
CISPEP   9 VAL E  109    THR E  110          0         8.42                     
CISPEP  10 TYR E  143    PRO E  144          0         1.47                     
SITE     1 AC1 11 ARG A 249  THR A 306  PHE A 310  LYS A 312                    
SITE     2 AC1 11 ARG A 313  ASN A 387  SER A 388  SER A 390                    
SITE     3 AC1 11 LYS A 391  ILE A 392  LEU A 395                               
SITE     1 AC2 11 VAL A 257  THR A 261  ILE B 255  THR B 256                    
SITE     2 AC2 11 SER C 267  THR C 271  VAL D 257  THR D 261                    
SITE     3 AC2 11 ALA E 252  THR E 256  LEU E 259                               
SITE     1 AC3  9 ARG D 249  PHE D 310  LYS D 312  ARG D 313                    
SITE     2 AC3  9 ASN D 387  SER D 388  SER D 390  LYS D 391                    
SITE     3 AC3  9 ILE D 392                                                     
SITE     1 AC4 11 ASN A 111  THR A 113  SER C 100  ASN C 101                    
SITE     2 AC4 11 VAL C 103  GLY C 104  LYS C 105  TRP C 123                    
SITE     3 AC4 11 PRO C 127  ASN D  28  NAG D 502                               
SITE     1 AC5  3 PRO B  78  ASN B  80  HIS B 119                               
SITE     1 AC6  6 ASN B 149  ARG B 192  SER B 195  ARG B 196                    
SITE     2 AC6  6 SER B 211  ARG B 213                                          
SITE     1 AC7  3 TRP C 183  SER C 187  ASN C 208                               
SITE     1 AC8  5 LEU A  89  MAN A 507  ASN D 111  PRO D 115                    
SITE     2 AC8  5 ASP E  89                                                     
SITE     1 AC9  3 PRO E  78  ASN E  80  HIS E 119                               
SITE     1 AD1  5 ASN E 149  GLU E 190  ARG E 192  ARG E 196                    
SITE     2 AD1  5 SER E 211                                                     
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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