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Database: PDB
Entry: 6IF3
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HEADER    ENDOCYTOSIS                             18-SEP-18   6IF3              
TITLE     COMPLEX STRUCTURE OF RAB35 AND ITS EFFECTOR ACAP2                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ARF-GAP WITH COILED-COIL, ANK REPEAT AND PH DOMAIN-        
COMPND   3 CONTAINING PROTEIN 2;                                                
COMPND   4 CHAIN: A;                                                            
COMPND   5 SYNONYM: CENTAURIN-BETA-2,CNT-B2;                                    
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: RAS-RELATED PROTEIN RAB-35;                                
COMPND   9 CHAIN: B;                                                            
COMPND  10 SYNONYM: GTP-BINDING PROTEIN RAY,RAS-RELATED PROTEIN RAB-1C;         
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ACAP2, CENTB2, KIAA0041;                                       
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 GENE: RAB35, RAB1C, RAY;                                             
SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  16 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)                                  
KEYWDS    RAB35, ACAP2, COMPLEX, CRYATAL STRUCTURE, ENDOCYTOSIS                 
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    L.LIN,J.ZHU,R.ZHANG                                                   
REVDAT   3   22-MAY-19 6IF3    1       JRNL                                     
REVDAT   2   10-APR-19 6IF3    1       JRNL                                     
REVDAT   1   03-APR-19 6IF3    0                                                
JRNL        AUTH   L.LIN,Y.SHI,M.WANG,C.WANG,J.ZHU,R.ZHANG                      
JRNL        TITL   RAB35/ACAP2 AND RAB35/RUSC2 COMPLEX STRUCTURES REVEAL        
JRNL        TITL 2 MOLECULAR BASIS FOR EFFECTOR RECOGNITION BY RAB35 GTPASE.    
JRNL        REF    STRUCTURE                     V.  27   729 2019              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   30905672                                                     
JRNL        DOI    10.1016/J.STR.2019.02.008                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.8.4_1496                                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 28.05                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 96.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 54479                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.174                           
REMARK   3   R VALUE            (WORKING SET) : 0.172                           
REMARK   3   FREE R VALUE                     : 0.205                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.060                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2759                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 28.0536 -  4.0677    1.00     2876   153  0.1684 0.2024        
REMARK   3     2  4.0677 -  3.2302    1.00     2768   133  0.1434 0.1695        
REMARK   3     3  3.2302 -  2.8223    1.00     2695   148  0.1506 0.1773        
REMARK   3     4  2.8223 -  2.5644    1.00     2686   170  0.1624 0.1845        
REMARK   3     5  2.5644 -  2.3807    1.00     2708   137  0.1650 0.1972        
REMARK   3     6  2.3807 -  2.2404    1.00     2648   134  0.1608 0.1961        
REMARK   3     7  2.2404 -  2.1283    1.00     2676   138  0.1666 0.1921        
REMARK   3     8  2.1283 -  2.0357    1.00     2672   137  0.1639 0.2095        
REMARK   3     9  2.0357 -  1.9573    1.00     2671   138  0.1725 0.1841        
REMARK   3    10  1.9573 -  1.8898    1.00     2641   140  0.1733 0.2123        
REMARK   3    11  1.8898 -  1.8307    1.00     2644   136  0.1872 0.2375        
REMARK   3    12  1.8307 -  1.7784    1.00     2627   147  0.2003 0.2505        
REMARK   3    13  1.7784 -  1.7316    1.00     2632   148  0.2078 0.2591        
REMARK   3    14  1.7316 -  1.6893    1.00     2668   144  0.2119 0.2569        
REMARK   3    15  1.6893 -  1.6509    1.00     2618   129  0.2192 0.2760        
REMARK   3    16  1.6509 -  1.6158    0.99     2571   159  0.2396 0.2947        
REMARK   3    17  1.6158 -  1.5835    0.97     2546   127  0.2529 0.2927        
REMARK   3    18  1.5835 -  1.5536    0.88     2328   132  0.2763 0.3113        
REMARK   3    19  1.5536 -  1.5259    0.82     2167   116  0.2979 0.3370        
REMARK   3    20  1.5259 -  1.5000    0.71     1878    93  0.3230 0.3538        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.180            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 21.600           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 19.55                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 27.04                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.011           2736                                  
REMARK   3   ANGLE     :  1.255           3724                                  
REMARK   3   CHIRALITY :  0.057            424                                  
REMARK   3   PLANARITY :  0.007            485                                  
REMARK   3   DIHEDRAL  : 17.529           1017                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 13                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 605 THROUGH 627 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -14.3041  45.9180  17.9145              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1330 T22:   0.1182                                     
REMARK   3      T33:   0.1567 T12:   0.0028                                     
REMARK   3      T13:  -0.0019 T23:  -0.0567                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.3914 L22:   4.2633                                     
REMARK   3      L33:   6.3957 L12:   0.2799                                     
REMARK   3      L13:   1.6572 L23:  -3.5403                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0623 S12:  -0.2604 S13:   0.2449                       
REMARK   3      S21:   0.2701 S22:  -0.0074 S23:  -0.0589                       
REMARK   3      S31:  -0.2844 S32:   0.0586 S33:   0.0621                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 628 THROUGH 637 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -17.1472  37.9661  22.1799              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1827 T22:   0.1279                                     
REMARK   3      T33:   0.1332 T12:  -0.0074                                     
REMARK   3      T13:   0.0182 T23:   0.0088                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.7825 L22:   4.3620                                     
REMARK   3      L33:   8.7329 L12:  -3.2294                                     
REMARK   3      L13:   0.2040 L23:  -0.1435                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3330 S12:  -0.0539 S13:  -0.1224                       
REMARK   3      S21:   0.4150 S22:   0.1032 S23:   0.1176                       
REMARK   3      S31:   0.1355 S32:   0.0734 S33:   0.1541                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 638 THROUGH 695 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -17.1138  35.6502   8.4218              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1182 T22:   0.1328                                     
REMARK   3      T33:   0.1202 T12:  -0.0042                                     
REMARK   3      T13:   0.0155 T23:  -0.0128                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.3589 L22:   2.3847                                     
REMARK   3      L33:   2.4888 L12:  -0.0481                                     
REMARK   3      L13:   0.0163 L23:   0.2734                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0074 S12:   0.0206 S13:   0.0377                       
REMARK   3      S21:   0.0823 S22:  -0.0027 S23:   0.0162                       
REMARK   3      S31:   0.0138 S32:   0.0084 S33:   0.0186                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 696 THROUGH 709 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -22.0379  25.1292   4.3302              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2158 T22:   0.1209                                     
REMARK   3      T33:   0.1087 T12:  -0.0270                                     
REMARK   3      T13:   0.0046 T23:  -0.0047                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.6149 L22:   6.5265                                     
REMARK   3      L33:   8.2157 L12:   1.1240                                     
REMARK   3      L13:  -3.7264 L23:  -0.9620                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2737 S12:   0.4115 S13:  -0.0007                       
REMARK   3      S21:  -0.2095 S22:   0.0393 S23:   0.1893                       
REMARK   3      S31:   0.3621 S32:  -0.3199 S33:   0.2468                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 710 THROUGH 738 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -16.3935  24.9009  -5.9817              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1349 T22:   0.1495                                     
REMARK   3      T33:   0.1099 T12:  -0.0183                                     
REMARK   3      T13:   0.0126 T23:  -0.0261                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.9549 L22:   6.2131                                     
REMARK   3      L33:   3.2310 L12:  -2.7895                                     
REMARK   3      L13:   0.6063 L23:   0.3599                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0369 S12:   0.0856 S13:  -0.3277                       
REMARK   3      S21:   0.1130 S22:  -0.0211 S23:   0.0493                       
REMARK   3      S31:   0.1532 S32:   0.1093 S33:   0.0461                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 739 THROUGH 757 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.9853  27.1752  -6.8363              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1785 T22:   0.2660                                     
REMARK   3      T33:   0.2752 T12:  -0.0108                                     
REMARK   3      T13:   0.0269 T23:  -0.0529                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.7798 L22:   1.9550                                     
REMARK   3      L33:   5.9989 L12:   1.1555                                     
REMARK   3      L13:  -0.0104 L23:  -2.6995                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0706 S12:   0.1998 S13:  -0.6184                       
REMARK   3      S21:  -0.2732 S22:   0.1189 S23:  -0.3310                       
REMARK   3      S31:   0.2667 S32:   0.3684 S33:   0.0637                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 758 THROUGH 769 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -17.3045  41.8542  -6.1466              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3757 T22:   0.6036                                     
REMARK   3      T33:   0.5403 T12:   0.0623                                     
REMARK   3      T13:   0.0540 T23:  -0.0664                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.4280 L22:   5.0653                                     
REMARK   3      L33:   7.6520 L12:  -5.1269                                     
REMARK   3      L13:  -5.7683 L23:   4.9660                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.9597 S12:   0.2078 S13:   1.6827                       
REMARK   3      S21:  -0.6184 S22:  -0.5498 S23:  -0.4172                       
REMARK   3      S31:  -1.0053 S32:  -1.3294 S33:  -0.5682                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 2 THROUGH 30 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -11.9856  15.0619 -18.0824              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1825 T22:   0.1568                                     
REMARK   3      T33:   0.0884 T12:   0.0186                                     
REMARK   3      T13:   0.0303 T23:  -0.0304                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.4103 L22:   4.1713                                     
REMARK   3      L33:   2.1586 L12:  -2.7839                                     
REMARK   3      L13:   2.0290 L23:  -1.4731                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1338 S12:  -0.1352 S13:   0.0486                       
REMARK   3      S21:   0.1262 S22:   0.0253 S23:  -0.0767                       
REMARK   3      S31:   0.0601 S32:  -0.0853 S33:   0.1192                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 31 THROUGH 64 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -14.8339  14.4451 -15.8791              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1652 T22:   0.1831                                     
REMARK   3      T33:   0.1529 T12:   0.0008                                     
REMARK   3      T13:   0.0128 T23:   0.0033                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.6289 L22:   3.9432                                     
REMARK   3      L33:   0.8443 L12:  -1.8893                                     
REMARK   3      L13:   0.0726 L23:   0.6944                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0058 S12:  -0.0522 S13:   0.0294                       
REMARK   3      S21:   0.1074 S22:  -0.0529 S23:   0.0238                       
REMARK   3      S31:   0.0863 S32:  -0.1077 S33:   0.0531                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 65 THROUGH 82 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -12.1797  29.7367 -18.7320              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1718 T22:   0.1499                                     
REMARK   3      T33:   0.1438 T12:  -0.0106                                     
REMARK   3      T13:   0.0157 T23:   0.0294                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.0004 L22:   5.2385                                     
REMARK   3      L33:   2.9292 L12:  -4.8753                                     
REMARK   3      L13:  -3.9579 L23:   3.8686                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0788 S12:   0.1848 S13:   0.2422                       
REMARK   3      S21:  -0.1374 S22:   0.0328 S23:  -0.2074                       
REMARK   3      S31:  -0.1491 S32:  -0.0378 S33:  -0.1485                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 83 THROUGH 109 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -14.4506  26.0365 -31.2741              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2549 T22:   0.1642                                     
REMARK   3      T33:   0.1363 T12:  -0.0034                                     
REMARK   3      T13:  -0.0032 T23:   0.0070                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.7903 L22:   1.7628                                     
REMARK   3      L33:   3.3215 L12:   1.6624                                     
REMARK   3      L13:   3.4988 L23:   1.3742                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3462 S12:   0.2634 S13:   0.3575                       
REMARK   3      S21:  -0.4878 S22:   0.0987 S23:   0.0635                       
REMARK   3      S31:  -0.4309 S32:   0.0178 S33:   0.2635                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 110 THROUGH 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -15.8756  17.8862 -35.8568              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2408 T22:   0.1922                                     
REMARK   3      T33:   0.1572 T12:  -0.0053                                     
REMARK   3      T13:  -0.0090 T23:  -0.0195                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7261 L22:   2.2145                                     
REMARK   3      L33:   3.5742 L12:  -0.8839                                     
REMARK   3      L13:  -1.4565 L23:   0.4259                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1248 S12:   0.5250 S13:  -0.0657                       
REMARK   3      S21:  -0.3380 S22:  -0.0616 S23:   0.0784                       
REMARK   3      S31:  -0.0786 S32:  -0.2810 S33:   0.1704                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 143 THROUGH 178 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.5950  10.0483 -27.0799              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2448 T22:   0.1666                                     
REMARK   3      T33:   0.1927 T12:   0.0445                                     
REMARK   3      T13:   0.0236 T23:  -0.0320                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.3212 L22:   1.8839                                     
REMARK   3      L33:   2.5960 L12:   0.9087                                     
REMARK   3      L13:   1.0147 L23:   0.3674                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0212 S12:   0.0490 S13:  -0.3420                       
REMARK   3      S21:   0.0266 S22:  -0.0354 S23:  -0.1055                       
REMARK   3      S31:   0.2173 S32:   0.2052 S33:   0.0057                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6IF3 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 24-SEP-18.                  
REMARK 100 THE DEPOSITION ID IS D_1300009092.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-NOV-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRF                               
REMARK 200  BEAMLINE                       : BL19U1                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9785                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 6M                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 54675                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 96.7                               
REMARK 200  DATA REDUNDANCY                : 10.40                              
REMARK 200  R MERGE                    (I) : 0.11700                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 5.2000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.53                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 68.8                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.58400                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 3TW8, 3JUE                                           
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 42.74                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.15                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: POTASSIUM SODIUM TARTRATE, PEG 3350,     
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       22.37050            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       72.00400            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       26.73400            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       72.00400            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       22.37050            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       26.73400            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2810 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15950 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -27.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A   597                                                      
REMARK 465     PRO A   598                                                      
REMARK 465     GLY A   599                                                      
REMARK 465     SER A   600                                                      
REMARK 465     SER A   601                                                      
REMARK 465     LYS A   602                                                      
REMARK 465     HIS A   603                                                      
REMARK 465     LEU A   604                                                      
REMARK 465     GLY A   740                                                      
REMARK 465     LEU A   741                                                      
REMARK 465     TYR A   742                                                      
REMARK 465     GLY A   743                                                      
REMARK 465     GLN A   744                                                      
REMARK 465     PRO A   745                                                      
REMARK 465     GLY A   746                                                      
REMARK 465     GLY B    -5                                                      
REMARK 465     PRO B    -4                                                      
REMARK 465     GLY B    -3                                                      
REMARK 465     SER B    -2                                                      
REMARK 465     GLU B    -1                                                      
REMARK 465     PHE B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     GLN B   179                                                      
REMARK 465     GLN B   180                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A 736    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 737    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 739    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 747    CG   OD1  OD2                                       
REMARK 470     GLU A 748    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 766    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 126    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 170    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B 178    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HOH B   461     O    HOH B   476              1.97            
REMARK 500   O    HOH A   930     O    HOH B   497              2.09            
REMARK 500   O    HOH A   957     O    HOH A   979              2.10            
REMARK 500   O    HOH B   448     O    HOH B   471              2.12            
REMARK 500   O    HOH A   962     O    HOH A   968              2.15            
REMARK 500   OD1  ASN B   175     O    HOH B   301              2.17            
REMARK 500   NH2  ARG A   770     O    HOH A   801              2.17            
REMARK 500   O    HOH B   339     O    HOH B   477              2.19            
REMARK 500   OE1  GLU B   166     O    HOH B   302              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   O    HOH A   949     O    HOH A   973     4465     2.16            
REMARK 500   NH1  ARG A   696     O    GLU A   766     4565     2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASP B 123       16.38   -140.41                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH A 988        DISTANCE =  6.15 ANGSTROMS                       
REMARK 525    HOH A 989        DISTANCE =  6.16 ANGSTROMS                       
REMARK 525    HOH A 990        DISTANCE =  7.06 ANGSTROMS                       
REMARK 525    HOH A 991        DISTANCE =  7.48 ANGSTROMS                       
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG B 202  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 SER B  22   OG                                                     
REMARK 620 2 THR B  40   OG1  83.6                                              
REMARK 620 3 GTP B 201   O2G 175.1  92.3                                        
REMARK 620 4 GTP B 201   O2B  90.2 173.7  93.8                                  
REMARK 620 5 HOH B 332   O    87.6  90.8  89.9  87.9                            
REMARK 620 6 HOH B 322   O    87.1  89.2  95.4  91.5 174.7                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue GTP B 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue MG B 202                  
DBREF  6IF3 A  601   770  UNP    Q15057   ACAP2_HUMAN    601    770             
DBREF  6IF3 B    1   180  UNP    Q15286   RAB35_HUMAN      1    180             
SEQADV 6IF3 GLY A  597  UNP  Q15057              EXPRESSION TAG                 
SEQADV 6IF3 PRO A  598  UNP  Q15057              EXPRESSION TAG                 
SEQADV 6IF3 GLY A  599  UNP  Q15057              EXPRESSION TAG                 
SEQADV 6IF3 SER A  600  UNP  Q15057              EXPRESSION TAG                 
SEQADV 6IF3 GLY B   -5  UNP  Q15286              EXPRESSION TAG                 
SEQADV 6IF3 PRO B   -4  UNP  Q15286              EXPRESSION TAG                 
SEQADV 6IF3 GLY B   -3  UNP  Q15286              EXPRESSION TAG                 
SEQADV 6IF3 SER B   -2  UNP  Q15286              EXPRESSION TAG                 
SEQADV 6IF3 GLU B   -1  UNP  Q15286              EXPRESSION TAG                 
SEQADV 6IF3 PHE B    0  UNP  Q15286              EXPRESSION TAG                 
SEQADV 6IF3 LEU B   67  UNP  Q15286    GLN    67 ENGINEERED MUTATION            
SEQRES   1 A  174  GLY PRO GLY SER SER LYS HIS LEU ASN PRO GLY LEU GLN          
SEQRES   2 A  174  LEU TYR ARG ALA SER TYR GLU LYS ASN LEU PRO LYS MET          
SEQRES   3 A  174  ALA GLU ALA LEU ALA HIS GLY ALA ASP VAL ASN TRP ALA          
SEQRES   4 A  174  ASN SER GLU GLU ASN LYS ALA THR PRO LEU ILE GLN ALA          
SEQRES   5 A  174  VAL LEU GLY GLY SER LEU VAL THR CYS GLU PHE LEU LEU          
SEQRES   6 A  174  GLN ASN GLY ALA ASN VAL ASN GLN ARG ASP VAL GLN GLY          
SEQRES   7 A  174  ARG GLY PRO LEU HIS HIS ALA THR VAL LEU GLY HIS THR          
SEQRES   8 A  174  GLY GLN VAL CYS LEU PHE LEU LYS ARG GLY ALA ASN GLN          
SEQRES   9 A  174  HIS ALA THR ASP GLU GLU GLY LYS ASP PRO LEU SER ILE          
SEQRES  10 A  174  ALA VAL GLU ALA ALA ASN ALA ASP ILE VAL THR LEU LEU          
SEQRES  11 A  174  ARG LEU ALA ARG MET ASN GLU GLU MET ARG GLU SER GLU          
SEQRES  12 A  174  GLY LEU TYR GLY GLN PRO GLY ASP GLU THR TYR GLN ASP          
SEQRES  13 A  174  ILE PHE ARG ASP PHE SER GLN MET ALA SER ASN ASN PRO          
SEQRES  14 A  174  GLU LYS LEU ASN ARG                                          
SEQRES   1 B  186  GLY PRO GLY SER GLU PHE MET ALA ARG ASP TYR ASP HIS          
SEQRES   2 B  186  LEU PHE LYS LEU LEU ILE ILE GLY ASP SER GLY VAL GLY          
SEQRES   3 B  186  LYS SER SER LEU LEU LEU ARG PHE ALA ASP ASN THR PHE          
SEQRES   4 B  186  SER GLY SER TYR ILE THR THR ILE GLY VAL ASP PHE LYS          
SEQRES   5 B  186  ILE ARG THR VAL GLU ILE ASN GLY GLU LYS VAL LYS LEU          
SEQRES   6 B  186  GLN ILE TRP ASP THR ALA GLY LEU GLU ARG PHE ARG THR          
SEQRES   7 B  186  ILE THR SER THR TYR TYR ARG GLY THR HIS GLY VAL ILE          
SEQRES   8 B  186  VAL VAL TYR ASP VAL THR SER ALA GLU SER PHE VAL ASN          
SEQRES   9 B  186  VAL LYS ARG TRP LEU HIS GLU ILE ASN GLN ASN CYS ASP          
SEQRES  10 B  186  ASP VAL CYS ARG ILE LEU VAL GLY ASN LYS ASN ASP ASP          
SEQRES  11 B  186  PRO GLU ARG LYS VAL VAL GLU THR GLU ASP ALA TYR LYS          
SEQRES  12 B  186  PHE ALA GLY GLN MET GLY ILE GLN LEU PHE GLU THR SER          
SEQRES  13 B  186  ALA LYS GLU ASN VAL ASN VAL GLU GLU MET PHE ASN CYS          
SEQRES  14 B  186  ILE THR GLU LEU VAL LEU ARG ALA LYS LYS ASP ASN LEU          
SEQRES  15 B  186  ALA LYS GLN GLN                                              
HET    GTP  B 201      32                                                       
HET     MG  B 202       1                                                       
HETNAM     GTP GUANOSINE-5'-TRIPHOSPHATE                                        
HETNAM      MG MAGNESIUM ION                                                    
FORMUL   3  GTP    C10 H16 N5 O14 P3                                            
FORMUL   4   MG    MG 2+                                                        
FORMUL   5  HOH   *390(H2 O)                                                    
HELIX    1 AA1 ASN A  605  GLU A  616  1                                  12    
HELIX    2 AA2 ASN A  618  HIS A  628  1                                  11    
HELIX    3 AA3 THR A  643  GLY A  652  1                                  10    
HELIX    4 AA4 SER A  653  ASN A  663  1                                  11    
HELIX    5 AA5 GLY A  676  GLY A  685  1                                  10    
HELIX    6 AA6 HIS A  686  ARG A  696  1                                  11    
HELIX    7 AA7 ASP A  709  ALA A  717  1                                   9    
HELIX    8 AA8 ASN A  719  SER A  738  1                                  20    
HELIX    9 AA9 ASP A  752  SER A  758  1                                   7    
HELIX   10 AB1 GLY B   20  ASN B   31  1                                  12    
HELIX   11 AB2 LEU B   67  ARG B   71  5                                   5    
HELIX   12 AB3 ILE B   73  ARG B   79  1                                   7    
HELIX   13 AB4 SER B   92  ASN B   98  1                                   7    
HELIX   14 AB5 ASN B   98  CYS B  110  1                                  13    
HELIX   15 AB6 ASP B  124  LYS B  128  5                                   5    
HELIX   16 AB7 GLU B  131  GLY B  143  1                                  13    
HELIX   17 AB8 ASN B  156  ASP B  174  1                                  19    
SHEET    1 AA1 6 VAL B  43  ILE B  52  0                                        
SHEET    2 AA1 6 GLU B  55  THR B  64 -1  O  LEU B  59   N  ARG B  48           
SHEET    3 AA1 6 HIS B   7  GLY B  15  1  N  HIS B   7   O  LYS B  58           
SHEET    4 AA1 6 GLY B  83  ASP B  89  1  O  ILE B  85   N  LEU B  12           
SHEET    5 AA1 6 CYS B 114  ASN B 120  1  O  ASN B 120   N  TYR B  88           
SHEET    6 AA1 6 GLN B 145  GLU B 148  1  O  GLN B 145   N  LEU B 117           
LINK         OG  SER B  22                MG    MG B 202     1555   1555  2.16  
LINK         OG1 THR B  40                MG    MG B 202     1555   1555  2.07  
LINK         O2G GTP B 201                MG    MG B 202     1555   1555  2.01  
LINK         O2B GTP B 201                MG    MG B 202     1555   1555  2.09  
LINK        MG    MG B 202                 O   HOH B 332     1555   1555  2.09  
LINK        MG    MG B 202                 O   HOH B 322     1555   1555  2.07  
SITE     1 AC1 26 SER B  17  GLY B  18  VAL B  19  GLY B  20                    
SITE     2 AC1 26 LYS B  21  SER B  22  SER B  23  PHE B  33                    
SITE     3 AC1 26 GLY B  35  TYR B  37  THR B  40  GLY B  66                    
SITE     4 AC1 26 ASN B 120  LYS B 121  ASP B 123  SER B 150                    
SITE     5 AC1 26 ALA B 151  LYS B 152   MG B 202  HOH B 301                    
SITE     6 AC1 26 HOH B 322  HOH B 327  HOH B 332  HOH B 333                    
SITE     7 AC1 26 HOH B 410  HOH B 437                                          
SITE     1 AC2  5 SER B  22  THR B  40  GTP B 201  HOH B 322                    
SITE     2 AC2  5 HOH B 332                                                     
CRYST1   44.741   53.468  144.008  90.00  90.00  90.00 P 21 21 21    4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.022351  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.018703  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.006944        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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