HEADER TRANSCRIPTION 24-MAY-19 6K4N
TITLE CRYO-EM STRUCTURE OF P300
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: HISTONE ACETYLTRANSFERASE P300;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: UNP RESIDUES 1046-1664;
COMPND 5 SYNONYM: P300 HAT,E1A-ASSOCIATED PROTEIN P300,HISTONE
COMPND 6 BUTYRYLTRANSFERASE P300,HISTONE CROTONYLTRANSFERASE P300,PROTEIN
COMPND 7 PROPIONYLTRANSFERASE P300;
COMPND 8 EC: 2.3.1.48,2.3.1.-;
COMPND 9 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: EP300, P300;
SOURCE 6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;
SOURCE 7 EXPRESSION_SYSTEM_TAXID: 7108
KEYWDS TRANSCRIPTIONAL COACTIVATOR, HAT, AUTO-INHIBITED CLOSED CONFORMATION,
KEYWDS 2 TRANSCRIPTION
EXPDTA ELECTRON MICROSCOPY
MDLTYP CA ATOMS ONLY, CHAIN A
AUTHOR R.GHOSH,S.ROY,J.SENGUPTA
REVDAT 5 27-MAR-24 6K4N 1 REMARK
REVDAT 4 06-NOV-19 6K4N 1 CRYST1
REVDAT 3 28-AUG-19 6K4N 1 JRNL
REVDAT 2 31-JUL-19 6K4N 1 JRNL
REVDAT 1 19-JUN-19 6K4N 0
SPRSDE 19-JUN-19 6K4N 5XZS
JRNL AUTH R.GHOSH,S.KAYPEE,M.SHASMAL,T.K.KUNDU,S.ROY,J.SENGUPTA
JRNL TITL TUMOR SUPPRESSOR P53-MEDIATED STRUCTURAL REORGANIZATION OF
JRNL TITL 2 THE TRANSCRIPTIONAL COACTIVATOR P300.
JRNL REF BIOCHEMISTRY V. 58 3434 2019
JRNL REFN ISSN 0006-2960
JRNL PMID 31314496
JRNL DOI 10.1021/ACS.BIOCHEM.9B00333
REMARK 2
REMARK 2 RESOLUTION. 9.80 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 SOFTWARE PACKAGES : EMAN2, UCSF CHIMERA, EMAN2, SPIDER,
REMARK 3 SPIDER, SPIDER
REMARK 3 RECONSTRUCTION SCHEMA : BACK PROJECTION
REMARK 3
REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT
REMARK 3 PDB ENTRY : 4BHW
REMARK 3 REFINEMENT SPACE : NULL
REMARK 3 REFINEMENT PROTOCOL : NULL
REMARK 3 REFINEMENT TARGET : NULL
REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL
REMARK 3
REMARK 3 FITTING PROCEDURE : NULL
REMARK 3
REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS
REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 9.800
REMARK 3 NUMBER OF PARTICLES : 16152
REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE
REMARK 3 CORRECTION
REMARK 3
REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL
REMARK 3
REMARK 3 OTHER DETAILS: NULL
REMARK 4
REMARK 4 6K4N COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 28-MAY-19.
REMARK 100 THE DEPOSITION ID IS D_1300012249.
REMARK 245
REMARK 245 EXPERIMENTAL DETAILS
REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE
REMARK 245 SPECIMEN TYPE : NULL
REMARK 245
REMARK 245 ELECTRON MICROSCOPE SAMPLE
REMARK 245 SAMPLE TYPE : PARTICLE
REMARK 245 PARTICLE TYPE : POINT
REMARK 245 NAME OF SAMPLE : P300 PROTEIN
REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL
REMARK 245 SAMPLE SUPPORT DETAILS : NULL
REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL
REMARK 245 SAMPLE BUFFER : NULL
REMARK 245 PH : 8.00
REMARK 245 SAMPLE DETAILS : NULL
REMARK 245
REMARK 245 DATA ACQUISITION
REMARK 245 DATE OF EXPERIMENT : NULL
REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL
REMARK 245 TEMPERATURE (KELVIN) : NULL
REMARK 245 MICROSCOPE MODEL : FEI POLARA 300
REMARK 245 DETECTOR TYPE : FEI EAGLE (4K X 4K)
REMARK 245 MINIMUM DEFOCUS (NM) : 1500.00
REMARK 245 MAXIMUM DEFOCUS (NM) : 4500.00
REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 NOMINAL CS : 2.00
REMARK 245 IMAGING MODE : BRIGHT FIELD
REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 1500.00
REMARK 245 ILLUMINATION MODE : FLOOD BEAM
REMARK 245 NOMINAL MAGNIFICATION : 59000
REMARK 245 CALIBRATED MAGNIFICATION : 78894
REMARK 245 SOURCE : FIELD EMISSION GUN
REMARK 245 ACCELERATION VOLTAGE (KV) : 300
REMARK 245 IMAGING DETAILS : NULL
REMARK 247
REMARK 247 ELECTRON MICROSCOPY
REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON
REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE
REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES
REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION
REMARK 247 OF THE STRUCTURE FACTORS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 LEU A 1520
REMARK 465 GLU A 1521
REMARK 465 GLN A 1522
REMARK 465 GLU A 1523
REMARK 465 GLU A 1524
REMARK 465 GLU A 1525
REMARK 465 GLU A 1526
REMARK 465 ARG A 1527
REMARK 465 LYS A 1528
REMARK 465 ARG A 1529
REMARK 465 GLU A 1530
REMARK 465 GLU A 1531
REMARK 465 ASN A 1532
REMARK 465 THR A 1533
REMARK 465 SER A 1534
REMARK 465 ASN A 1535
REMARK 465 GLU A 1536
REMARK 465 SER A 1537
REMARK 465 THR A 1538
REMARK 465 ASP A 1539
REMARK 465 VAL A 1540
REMARK 465 THR A 1541
REMARK 465 LYS A 1542
REMARK 465 GLY A 1543
REMARK 465 ASP A 1544
REMARK 465 SER A 1545
REMARK 465 LYS A 1546
REMARK 465 ASN A 1547
REMARK 465 ALA A 1548
REMARK 465 LYS A 1549
REMARK 465 LYS A 1550
REMARK 465 LYS A 1551
REMARK 465 ASN A 1552
REMARK 465 ASN A 1553
REMARK 465 LYS A 1554
REMARK 465 LYS A 1555
REMARK 465 THR A 1556
REMARK 465 SER A 1557
REMARK 465 LYS A 1558
REMARK 465 ASN A 1559
REMARK 465 LYS A 1560
REMARK 465 SER A 1561
REMARK 465 SER A 1562
REMARK 465 LEU A 1563
REMARK 465 SER A 1564
REMARK 465 ARG A 1565
REMARK 465 GLY A 1566
REMARK 465 ASN A 1567
REMARK 465 LYS A 1568
REMARK 465 LYS A 1569
REMARK 465 LYS A 1570
REMARK 465 PRO A 1571
REMARK 465 GLY A 1572
REMARK 465 MET A 1573
REMARK 465 PRO A 1574
REMARK 465 ASN A 1575
REMARK 465 VAL A 1576
REMARK 465 SER A 1577
REMARK 465 ASN A 1578
REMARK 465 ASP A 1579
REMARK 465 LEU A 1580
REMARK 465 SER A 1581
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE
REMARK 500 CA HIS A 1261 CA GLY A 1368 1.40
REMARK 500 CA SER A 1136 CA ARG A 1234 1.84
REMARK 500 CA THR A 1135 CA TYR A 1191 1.85
REMARK 500 CA GLN A 1256 CA ASP A 1367 2.10
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: EMD-6792 RELATED DB: EMDB
REMARK 900 CRYO-EM STRUCTURE OF P300
REMARK 900 RELATED ID: EMD-6791 RELATED DB: EMDB
REMARK 900 CRYO-EM STRUCTURE OF P300-P53 PROTEIN COMPLEX
DBREF 6K4N A 1046 1664 UNP Q09472 EP300_HUMAN 1046 1664
SEQRES 1 A 619 LYS LYS ILE PHE LYS PRO GLU GLU LEU ARG GLN ALA LEU
SEQRES 2 A 619 MET PRO THR LEU GLU ALA LEU TYR ARG GLN ASP PRO GLU
SEQRES 3 A 619 SER LEU PRO PHE ARG GLN PRO VAL ASP PRO GLN LEU LEU
SEQRES 4 A 619 GLY ILE PRO ASP TYR PHE ASP ILE VAL LYS SER PRO MET
SEQRES 5 A 619 ASP LEU SER THR ILE LYS ARG LYS LEU ASP THR GLY GLN
SEQRES 6 A 619 TYR GLN GLU PRO TRP GLN TYR VAL ASP ASP ILE TRP LEU
SEQRES 7 A 619 MET PHE ASN ASN ALA TRP LEU TYR ASN ARG LYS THR SER
SEQRES 8 A 619 ARG VAL TYR LYS TYR CYS SER LYS LEU SER GLU VAL PHE
SEQRES 9 A 619 GLU GLN GLU ILE ASP PRO VAL MET GLN SER LEU GLY TYR
SEQRES 10 A 619 CYS CYS GLY ARG LYS LEU GLU PHE SER PRO GLN THR LEU
SEQRES 11 A 619 CYS CYS TYR GLY LYS GLN LEU CYS THR ILE PRO ARG ASP
SEQRES 12 A 619 ALA THR TYR TYR SER TYR GLN ASN ARG TYR HIS PHE CYS
SEQRES 13 A 619 GLU LYS CYS PHE ASN GLU ILE GLN GLY GLU SER VAL SER
SEQRES 14 A 619 LEU GLY ASP ASP PRO SER GLN PRO GLN THR THR ILE ASN
SEQRES 15 A 619 LYS GLU GLN PHE SER LYS ARG LYS ASN ASP THR LEU ASP
SEQRES 16 A 619 PRO GLU LEU PHE VAL GLU CYS THR GLU CYS GLY ARG LYS
SEQRES 17 A 619 MET HIS GLN ILE CYS VAL LEU HIS HIS GLU ILE ILE TRP
SEQRES 18 A 619 PRO ALA GLY PHE VAL CYS ASP GLY CYS LEU LYS LYS SER
SEQRES 19 A 619 ALA ARG THR ARG LYS GLU ASN LYS PHE SER ALA LYS ARG
SEQRES 20 A 619 LEU PRO SER THR ARG LEU GLY THR PHE LEU GLU ASN ARG
SEQRES 21 A 619 VAL ASN ASP PHE LEU ARG ARG GLN ASN HIS PRO GLU SER
SEQRES 22 A 619 GLY GLU VAL THR VAL ARG VAL VAL HIS ALA SER ASP LYS
SEQRES 23 A 619 THR VAL GLU VAL LYS PRO GLY MET LYS ALA ARG PHE VAL
SEQRES 24 A 619 ASP SER GLY GLU MET ALA GLU SER PHE PRO TYR ARG THR
SEQRES 25 A 619 LYS ALA LEU PHE ALA PHE GLU GLU ILE ASP GLY VAL ASP
SEQRES 26 A 619 LEU CYS PHE PHE GLY MET HIS VAL GLN GLU TYR GLY SER
SEQRES 27 A 619 ASP CYS PRO PRO PRO ASN GLN ARG ARG VAL TYR ILE SER
SEQRES 28 A 619 TYR LEU ASP SER VAL HIS PHE PHE ARG PRO LYS CYS LEU
SEQRES 29 A 619 ARG THR ALA VAL TYR HIS GLU ILE LEU ILE GLY TYR LEU
SEQRES 30 A 619 GLU TYR VAL LYS LYS LEU GLY TYR THR THR GLY HIS ILE
SEQRES 31 A 619 TRP ALA CYS PRO PRO SER GLU GLY ASP ASP TYR ILE PHE
SEQRES 32 A 619 HIS CYS HIS PRO PRO ASP GLN LYS ILE PRO LYS PRO LYS
SEQRES 33 A 619 ARG LEU GLN GLU TRP TYR LYS LYS MET LEU ASP LYS ALA
SEQRES 34 A 619 VAL SER GLU ARG ILE VAL HIS ASP TYR LYS ASP ILE PHE
SEQRES 35 A 619 LYS GLN ALA THR GLU ASP ARG LEU THR SER ALA LYS GLU
SEQRES 36 A 619 LEU PRO TYR PHE GLU GLY ASP PHE TRP PRO ASN VAL LEU
SEQRES 37 A 619 GLU GLU SER ILE LYS GLU LEU GLU GLN GLU GLU GLU GLU
SEQRES 38 A 619 ARG LYS ARG GLU GLU ASN THR SER ASN GLU SER THR ASP
SEQRES 39 A 619 VAL THR LYS GLY ASP SER LYS ASN ALA LYS LYS LYS ASN
SEQRES 40 A 619 ASN LYS LYS THR SER LYS ASN LYS SER SER LEU SER ARG
SEQRES 41 A 619 GLY ASN LYS LYS LYS PRO GLY MET PRO ASN VAL SER ASN
SEQRES 42 A 619 ASP LEU SER GLN LYS LEU TYR ALA THR MET GLU LYS HIS
SEQRES 43 A 619 LYS GLU VAL PHE PHE VAL ILE ARG LEU ILE ALA GLY PRO
SEQRES 44 A 619 ALA ALA ASN SER LEU PRO PRO ILE VAL ASP PRO ASP PRO
SEQRES 45 A 619 LEU ILE PRO CYS ASP LEU MET ASP GLY ARG ASP ALA PHE
SEQRES 46 A 619 LEU THR LEU ALA ARG ASP LYS HIS LEU GLU PHE SER SER
SEQRES 47 A 619 LEU ARG ARG ALA GLN TRP SER THR MET CYS MET LEU VAL
SEQRES 48 A 619 GLU LEU HIS THR GLN SER GLN ASP
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
