#=GF ID   Cas9_C
#=GF AC   PF18525.5
#=GF DE   Cas9 C-terminal domain
#=GF AU   El-Gebali S;0000-0003-1378-5495
#=GF SE   ECOD:EUF02052
#=GF GA   25.00 25.00;
#=GF TC   25.50 50.40;
#=GF NC   24.20 17.20;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -E 1000 --cpu 4 -Z 75585367 HMM pfamseq
#=GF TP   Domain
#=GF RN   [1]
#=GF RM   24505130
#=GF RT   Structures of Cas9 endonucleases reveal RNA-mediated
#=GF RT   conformational activation.
#=GF RA   Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders
#=GF RA   C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E,
#=GF RA   Nogales E, Doudna JA;
#=GF RL   Science. 2014;343:1247997.
#=GF DR   INTERPRO; IPR041217;
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   This is the C-terminal domain of Cas9 enzymes found in
#=GF CC   actinobacteria [1].
#=GF SQ   43
#=GS E6KTN8_9ACTO/1063-1181      AC E6KTN8.1
#=GS U7MNV4_9CORY/186-293        AC U7MNV4.1
#=GS A0A2M9HGW5_9BIFI/1026-1148  AC A0A2M9HGW5.1
#=GS A0A1G9X1C3_9ACTO/982-1088   AC A0A1G9X1C3.1
#=GS A0A239J842_9MICO/985-1103   AC A0A239J842.1
#=GS F9PLJ4_9ACTO/990-1095       AC F9PLJ4.1
#=GS A0A095Y326_9ACTO/996-1103   AC A0A095Y326.1
#=GS A0LWB3_ACIC1/1009-1128      AC A0LWB3.1
#=GS A0A5B8NRZ4_9CORY/998-1111   AC A0A5B8NRZ4.1
#=GS A0A7G7CLW5_9CORY/993-1104   AC A0A7G7CLW5.1
#=GS U1SJC0_9BIFI/1017-1132      AC U1SJC0.1
#=GS A0A2N6UX38_9ACTO/1007-1119  AC A0A2N6UX38.1
#=GS CAS9_CORDI/977-1084         AC Q6NKI3.1
#=GS A0A1Q5Q1I4_9ACTO/979-1085   AC A0A1Q5Q1I4.1
#=GS A0A7K1J561_9BIFI/512-627    AC A0A7K1J561.1
#=GS A0A080N644_9BIFI/1114-1237  AC A0A080N644.1
#=GS A0A418USS3_9ACTO/984-1091   AC A0A418USS3.1
#=GS A0A5J5E318_9BIFI/995-1113   AC A0A5J5E318.1
#=GS A0A542SPU9_9MICO/998-1103   AC A0A542SPU9.1
#=GS A0A840DQS7_9MICO/615-731    AC A0A840DQS7.1
#=GS A0A4Q9UYW5_9ACTO/982-1090   AC A0A4Q9UYW5.1
#=GS A0A5N5RHT9_9BIFI/1015-1134  AC A0A5N5RHT9.1
#=GS A0A7T0PE07_9ACTO/994-1101   AC A0A7T0PE07.1
#=GS E0DGS4_9CORY/982-1089       AC E0DGS4.1
#=GS A0A7K1UER3_9MICC/1005-1108  AC A0A7K1UER3.1
#=GS A0A1Q5PL82_9ACTO/969-1076   AC A0A1Q5PL82.1
#=GS A0A2M9HRX4_9BIFI/1017-1136  AC A0A2M9HRX4.1
#=GS A0A087A6R2_9BIFI/1016-1136  AC A0A087A6R2.1
#=GS K0YTG1_9ACTO/1007-1114      AC K0YTG1.1
#=GS W5IIZ2_SCAIO/1036-1164      AC W5IIZ2.1
#=GS A0A1R4G7C0_9MICO/1021-1137  AC A0A1R4G7C0.1
#=GS A0A0F6QV12_9CORY/988-1095   AC A0A0F6QV12.1
#=GS C0W0W4_9ACTO/999-1105       AC C0W0W4.1
#=GS A0A6I0FGN0_9CORY/997-1104   AC A0A6I0FGN0.1
#=GS A0A3A9AXD7_9BACT/1117-1255  AC A0A3A9AXD7.1
#=GS A0A3G6J4Z7_9CORY/999-1104   AC A0A3G6J4Z7.1
#=GS W5Y2L4_9CORY/984-1100       AC W5Y2L4.1
#=GS A0A087EJZ4_9BIFI/1021-1136  AC A0A087EJZ4.1
#=GS A0A6I1GJF2_9BIFI/960-1079   AC A0A6I1GJF2.1
#=GS A0A7Y0HSP3_9BIFI/1022-1144  AC A0A7Y0HSP3.1
#=GS A0A7Z0VUF7_9ACTO/976-1080   AC A0A7Z0VUF7.1
#=GS A0A6B8W6G1_9CORY/985-1091   AC A0A6B8W6G1.1
#=GS A0A430FRV1_9BIFI/1020-1135  AC A0A430FRV1.1
E6KTN8_9ACTO/1063-1181                 .........GDEIQIDPT.........K..FPKQSIGKFLKECGPVS........SWRVSALDTPSKITLKPRLLSNEPL.............LKTSRVGghesd.............lvvaecVEK.IM......KKTG..WVVEINALCQSG...LIRVIRRNALGEVRTSPK..SGLPISLNL-r..
U7MNV4_9CORY/186-293                   .........DDELVIDTA.........K..FNTGQIAEMQSEFGQIR........RWRLDGFYSDTKFRLRPLQMSAEGL.............PEDSTDG........................AKK.VI......DKPG..WLPAVNKLLALG...NVTVVRRDALGRVRLISA..AHLPISWKV-d..
A0A2M9HGW5_9BIFI/1026-1148             .........GDEIELDLS.........A.aPLSGQIGEFMQFFDGMNdegrnacrRWVVDGFYSPSKLRLRPRMLASEGL.............KKLTEFGnie.................ipdgVDK.II......NRQG..WFPAIG-VIDRY...RPRIIRRNAFGEPRWTSS..SGLPVSWSW-p..
A0A1G9X1C3_9ACTO/982-1088              .........GDELEISVS.........R..FTTGQIGSFLAEYPDTT........RWRVHGFFNESRLRLRPSQMSSEGL.............ADDAPDA........................VRK.TL......SIPG..WLPAVN-VLCSS...HPAVIRRDALGRPRMTSA..SRLPVSWVV-e..
A0A239J842_9MICO/985-1103              .........GDELLLDMS.........S..QTKGQIGELLADYPGTV........RWRVSGFPHISKLRLRPRLLAAEGLpdpia..stndsdAEQTSSG........................TRM.IL......AGQG..WRPGVNVIFDSC...DAVVIRRDTLGRVRLSSH..AHLPASWSV-r..
F9PLJ4_9ACTO/990-1095                  .........GDELHIPVE.........T..FTTHKIGSFLKDYPNTT........RWRICGYDSETMLILKPRILSAEGL.............-NNSSDI........................AEY.VI......KNKG..WRVAINKL-AKV...HPTVVRRDALGRPRHSSR..SNLPVTWSI-e..
A0A095Y326_9ACTO/996-1103              .........GDELQIETD.........K..YLKGFVGEVLAQYPELT........SWRVAGFPDPAKLRLRPYLLSVEGL.............EKGSSKE........................LSD.LL......EGQG..WRPAINVLLASG...VVSVVRRNCLGEPRSNSQ..NSLPITQ---llr
A0LWB3_ACIC1/1009-1128                 .........GDELDFGPE.........GvtTAAGDLSMFLKYFPER-........HWVVTGFEDDKRINLKPAFLSAEQAev........lrtE-----Rsdrp...............dtlteAGE.ILa....qFFPR.cWRATVAKVLCHP...GLTVIRRTALGQPRWR-R..GHLPYSWRP-w..
A0A5B8NRZ4_9CORY/998-1111              .........DDELMLDTA.........K..IATEKVITLLDKFGDIR........RWRIRGFESATQLCLRPAQLSAEGL.............SKDALPD........................LKE.IIgspdggKTHG..WRSAINKIFGEA...DPVIIRRDVHGRVRVSSS..RGLPVTYRM-k..
A0A7G7CLW5_9CORY/993-1104              .........DDELHINPH.........P..FNSGQVGNFQQEIGPTT........RWRVDGFYSNSRFRLRPLQLSAEGL.............KDKNGVLky...................hadVAK.VV......DSRG..WITAVNVLWGAG...DVVVVRRDALGRVRLRSA..AHLPITWK--a..
U1SJC0_9BIFI/1017-1132                 .........GDELRLDLS.........Q.tDYTGQVQEFAQVLANVAeae..vytTWVVDGFCDPAKLRLRPSYVSSEGV.............KSFDEAE........................QSR.LLk....vIDKG..WRPAIN-ALSAY...YPTVVRRNGLGEIRWKSN..AGLPMSYRWH...
A0A2N6UX38_9ACTO/1007-1119             ........g-TELELDLS.........HsyFHKNAIGEFLNDFPDNK........RWVVKRHDSNSTITLRPLIISSEGL.............DKFDLSD.......................gSLQ.IIs....tKGKG..WKIAVNALLGNS...LVKKIHRNALGEERETSN..CNLPVTT---vlk
CAS9_CORDI/977-1084                    .........DDELVVDTS.........K..IATDQVKAVEAELGTIR........RWRVDGFFSPSKLRLRPLQMSKEGI.............KKESAPE........................LSK.II......DRPG..WLPAVNKLFSDG...NVTVVRRDSLGRVRLEST..AHLPVTWKVQ...
A0A1Q5Q1I4_9ACTO/979-1085              .........GDEIRLDAS.........Q..VAAGKIAEFMKEYPTIR........SWRLDGFFSNSKLRLRPILLAAEGL.............SENASEA........................SKA.II......DRPG..WVPAVNVVFGQL...GAQIVRRTTLGELRTREV..SGMPISWSV-...
A0A7K1J561_9BIFI/512-627               .........GDEIEVEFN.........D..QGNNAIASFKNFFEHELsvspdvvnKWVVAGLMEPTKIRLRPSILSQEGM.............DRIKEAHpvda................silhDVE.SI......FDKG..WRMAVG-TASKY...KMRVVRRNVFGEKRYTSK..N---------ic.
A0A080N644_9BIFI/1114-1237             .........DDELEIPAFanar.iteeQ.aINGSFTDRLLHSFPGTH........KFRFAGFSRNTEIAIRPVQLASEGL.............IETDENR........................KRQ.QLr....lTQPNteYSNSIKNVLKSGlhlKVNTLFQTGILVTRANSQ..GKQSIRFST-v..
A0A418USS3_9ACTO/984-1091              .........GDELEVPMS.........G.fSSKGKIGDFLVEYPDTR........RWRVHGFYSETRLRLRPSQIAAEGL.............PDDAPDA........................VHK.TL......GTPG..WVIAVN-ALGAS...HPRVVRRDTLGRPRTAST..AGLPVSWRI-e..
A0A5J5E318_9BIFI/995-1113              .........GDELHVDLS.........K.eVLGGQIKDFAGWCAAQGipg..ianSWVIDGLFAEAKLRLHPRTLASEGLvk........frdIQPIPDG........................VVK.IV......SGAG..WLPSID-VLGRM...GPAVIRRNVLGEPRWHSS..NGLPCSWSW-a..
A0A542SPU9_9MICO/998-1103              .........GDEIQVKVP.........-..IARGSIGEFLEAFPGIT........RWEVTGLESPTTLKVRPLLLSKEAT.............PADLSEN........................ATT.VI......-QKA..WRPKINVLMQMP...GLKVVRRDTLGRERQESA..AHLPVSWSP-q..
A0A840DQS7_9MICO/615-731               ........g-TELAFDSS.........AfeSGSGALKDFLTILPETN........TWTITGFYDTARITIKPRQLSAEGL.............ENQNNALlan..................nqaVCR.IL......GVKG..LQISLN-VLLQQ...TVEVIHRNALGEKRYGDAasNSLPTTVQ--lk.
A0A4Q9UYW5_9ACTO/982-1090              .........GDEMVIDIS.........NpkLISGQIKEMLDDFPGQN........RFRVAGFYSNARLRLRPAMLSAEGL.............KDQHCES........................SYK.II......DGGG..WFPAINVICQYG...SPHVIRRSALGVPRTHSK..HGLPESWH--a..
A0A5N5RHT9_9BIFI/1015-1134             .........GDEIEMDFS.........G.vKTDGQIGEYLLFFSQFGgge.iagkRWVVDGFFSNSRLRLHPRYIAAEGL.............AKAFEESai...................pdgVQK.IV......AGQG..WLPSVN-VAGAT...AICTIRRNAFGERRFLAK..GHMPCSWQWK...
A0A7T0PE07_9ACTO/994-1101              .........GDELRLDPS.........S..FNTHAIGTFFSEYPRCQ........SWRIAGFPSPSRFRLRPMVLSGEGL.............PEGAGEA........................IQT.IT......FDRG..WLSTVNTLLSTT...ELQVVRRNALGERRITQK..TSLPSSYV--lr.
E0DGS4_9CORY/982-1089                  .........DDELLIDTS.........S..FDTPGIVKLREEYGPVN........RWRLAGLTSVTRLNLRPLYLSKEGL.............KPNVNPE........................IKK.II......DNRS..WIITVNKLFSSG...HVKIIRRDALGRPRLHSA..AHLPICWEV-k..
A0A7K1UER3_9MICC/1005-1108             .........GDEIEVDVS.........MaaQGKDKFASFLRSTPEH-........RWTVSGLKMSRIISLRPLFLSSEGL.............DNNSDPL........................LRE.VV......ERTL..VNPGVV----LP...LVKIVRRDALGRPRHFS-..RHLPHSFS--pm.
A0A1Q5PL82_9ACTO/969-1076              .........DDELLVDTS.........K..HGSADMSVLTEEYGKVS........RWTIEGFFDSGRLRLRPAQLSSEGL.............SENSPNS........................IWK.LL......DRPG..WLASVNKLFENS...DTRLIRRDSLGNERVETL..SGLPVSWSL-e..
A0A2M9HRX4_9BIFI/1017-1136             .........GDEIEMDFS.........E.sDAGGQIGEYLQFFSQLNggd.iagtHWVVDGFFSNSRLRLHPRYIASEGM.............SKSFADLsv...................pdgVQK.IV......AGQG..WLPSVN-VVAET...AIRTVRRNAFGELRFSAK..SHMPCSWRWK...
A0A087A6R2_9BIFI/1016-1136             .........GDELRVEFP.........RetALNGQIGEFVDWCDGSDsas.taqcSWVLDGFFSNKQLRLHPRLLASEGLmk........lgkNQEIPDG........................VQK.VV......AKTG..WISTVD-VFGGF...SPTVIRRNTLGEARWRSQ..SGLPCSWRW-f..
K0YTG1_9ACTO/1007-1114                 .........GDELKIDLS.........G..FTTGQIETFNETYPQAT........RWEVTGFPTKTQLRLKPLLIASEGL.............ADDVPDD........................IVK.IV......KGPG..WWPAIDKLLKIG...NVVLYRRDTHGKPRVVSH..RSLPLSSKLQ...
W5IIZ2_SCAIO/1036-1164                 .........GDELVLDLKgdksndeggS..RLSGQVGEFVQKFEEDSkvnsqivnRWTVDGFPDEAKLRLRPSMFAGEGL.............KNMKSELve...................naaIKN.IL......SKTG..WRVSVD-VISKK...SPVVLRRNAFGEPRWKTN..AGLPVSYRWQ...
A0A1R4G7C0_9MICO/1021-1137             .........GDELLIDPY.........E.wRGANETGRFMDEWPER-........HWRLSGWEQLGVANIKPVLLSEEGI.............RKKAEDTsdrl...............vdvstLTK.KV......LKAS..LRITASQLWSTP...STQVIRRTATGEPRQNIS..SGLPTSWS--pi.
A0A0F6QV12_9CORY/988-1095              .........DDELLIDTS.........S..FNSGQIAEMQAEFGMVR........RWRLDGFVSPSKLRLRPLQMSGEGL.............APGASAG........................SRK.IV......DQPG..WRPAVNKLLSAG...NVTIIRRNSLGQVRLNSN..AHLPTTWKV-d..
C0W0W4_9ACTO/999-1105                  .........GDELLLDLS.........S..ETSGQIAELQQDFPGTT........HWTVAGFFSPSRLRLRPVYLAQEGL.............GEDVSEG........................SKS.II......AGQG..WRPAVNKVFGSA...MPEVIRRDGLGRKRRFSY..SGLPVSWQ--g..
A0A6I0FGN0_9CORY/997-1104              .........GDELIFDTE.........K..LATGQISRYLKEFGPTK........HWRIRGLNTDIQLRLRPAQLSAEGL.............TSETSSD........................SKK.II......DNPG..WRPTVNKIFSAG...NVTLIRRDIHGRVRITDH..NGLPTSVKI-s..
A0A3A9AXD7_9BACT/1117-1255             nsdsffdpe---------.........-..-KNKKINLIMRAFPECR........EFVVTGFGTNAKLTVRPAQISSENLpdykeywrdalgrQ-----KnkevnsdmesdsvefasgeftkeeLKT.LFe....aLEKG..VPLAIN-ALMQT...SPWVVYRNTLGQTRYKSN..NHMPTSWLV-e..
A0A3G6J4Z7_9CORY/999-1104              .........GDELHLNPT.........V..L-PKSVTPLLEEFGHLH........RWTIDGFPDERRIRLRSRLLSGEGA.............-ENLPEE........................CRK.VL......TGRG..VLTSVNALLAQG...ELMVVRRDALGRVRLGSR..ANLPVSWRL-d..
W5Y2L4_9CORY/984-1100                  .........DDELLIDPT.........P..FSDGQVGNAQQELGPIR........RWRIDGFPLNSKFRLRPIQMSTEGL.............DLKAPSGvkkd...............rfgpdVRK.IV......DSPG..WRPAVNKLFSSG...KVTVIRRDALGRPRLESA..AHLPISWKV-e..
A0A087EJZ4_9BIFI/1021-1136             .........GDEIRLNLG.........K.vNYTGQVKEYEEVLRDSSsph..iysSWVIDGYYSPSRLRLRPLFLSSEGI.............DKMPIEQ........................QGV.LGk....mFKPG..WLPAVD-VLSSF...EPEVLRRNGLGEPRWSSN..SGLPVSYRWR...
A0A6I1GJF2_9BIFI/960-1079              .........GDELLVDFS.........R.gKINGKNAKFVNWCNETGate.aiyeSWTLAGFDTNSKLKLRPTVLAKEGL.............SNVPEHVkv...................pveVGG.IL......GDSG..WRPSVD-MTGTY...MPIVIRRNALGELRWKSK..AGLPCSWRW-a..
A0A7Y0HSP3_9BIFI/1022-1144             .........GDTIRVDFA.........R.sSISGQIEELINFFDSGHdedcnaynSWVVSGFFSESRLRLRPQLLAAEGI.............SNLQKYNnie.................vpegMSK.IV......TGQG..WLPSVD-AIAKF...FPQVVRVNTFGESREKSR..HGLPTSWKW-c..
A0A7Z0VUF7_9ACTO/976-1080              .........GDTLRLEST.........D..FKAARIRTFFDTYPVR-........EWTVTGFESAGMMNLRPTLLSSEGL.............PDDAPDD........................VRV.VL......-KRC..WRVSVNDLMTVG...HPSVVRRNTLGEVRMK-D..TKLPATVRL-a..
A0A6B8W6G1_9CORY/985-1091              .........DDEIQVDMA.........T..FNTGQIGAAQEQLGEIT........SWNLDGFFSNSRLRLRPRLFSAEGL.............KDDAVSD........................VRK.VL......DTTG..WLPAINKVFGDG...KAVVIRRDSLGRVRLESA..AHLPVSWK--a..
A0A430FRV1_9BIFI/1020-1135             ........n-DVIELHFG.........E..AEKSQIGDFLDFARPDSsinpgianKWVLVGMPEAARLKLRPLYFASEGL.............QNIENVE........................KSVcQI......LGNG..WRPTFG-VVAEH...HARILRRNALGDQRITSE..AHLPVSWELQ...
#=GC seq_cons                          .........GDELplDhu.........p..hssGpIucahppasptp........+WplsGF.oso+LRLRPhhLSuEGL.............pcpssss........................spc.ll......sppG..WhsuVN.lhups...pspVlRRsuLGcsRhpSp..utLPsSWph....
//