#=GF ID FokI_dom_2
#=GF AC PF02980.20
#=GF DE FokI, recognition domain, subdomain 2
#=GF PI FokI_C;
#=GF AU Griffiths-Jones SR;0000-0001-6043-807X
#=GF SE Structural domain
#=GF GA 25.00 25.00;
#=GF TC 26.00 63.20;
#=GF NC 24.90 20.90;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP Domain
#=GF CL CL0123
#=GF RN [1]
#=GF RM 9724743
#=GF RT Structure of FokI has implications for DNA cleavage.
#=GF RA Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK;
#=GF RL Proc Natl Acad Sci U S A. 1998;95:10564-10569.
#=GF RN [2]
#=GF RM 10508668
#=GF RT Type II restriction endonucleases: structural, functional and
#=GF RT evolutionary relationships.
#=GF RA Kovall RA, Matthews BW;
#=GF RL Curr Opin Chem Biol 1999;3:578-583.
#=GF RN [3]
#=GF RM 9214510
#=GF RT Structure of the multimodular endonuclease FokI bound to DNA.
#=GF RA Wah DA, Hirsch JA, Dorner LF, Schildkraut I, Aggarwal AK;
#=GF RL Nature. 1997;388:97-100.
#=GF DR INTERPRO; IPR004233;
#=GF DR SCOP; 2fok; fa;
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC Type IIS restriction endonuclease FokI is a member of an unusual
#=GF CC class of bipartite restriction enzymes that recognises the
#=GF CC double-stranded DNA sequence 5'-GGATG-3' and cleave DNA
#=GF CC phosphodiester groups 9 base pairs away on this strand and 13
#=GF CC base pairs away on the complementary strand [1]. FokI contains
#=GF CC amino- and carboxy-terminal domains corresponding to the DNA-
#=GF CC recognition and cleavage functions, respectively. The
#=GF CC recognition domain is made of three smaller subdomains (D1, D2
#=GF CC and D3) which are evolutionarily related to the
#=GF CC helix-turn-helix-containing DNA-binding domain of the catabolite
#=GF CC gene activator protein CAP [3]. This entry represents the
#=GF CC subdomain D2 of FokI. D1 and D2 make almost all of the
#=GF CC base-specific contacts with the DNA [3].
#=GF SQ 31
#=GS A0A1K1PVB9_RUMFL/148-283 AC A0A1K1PVB9.1
#=GS A0A174M7L2_9CLOT/147-281 AC A0A174M7L2.1
#=GS A0A1Q2MCL2_9BACT/143-285 AC A0A1Q2MCL2.1
#=GS I3DCT9_9PAST/154-285 AC I3DCT9.1
#=GS A0A8J6TVX0_9FIRM/165-298 AC A0A8J6TVX0.1
#=GS D9R4Y5_LACSW/147-282 AC D9R4Y5.1
#=GS A0A137SQ27_9FIRM/147-281 AC A0A137SQ27.1
#=GS G8PBE9_PEDCP/181-320 AC G8PBE9.1
#=GS A0A0F2J3F6_9BACT/144-283 AC A0A0F2J3F6.1
#=GS A0A1Y1RMG1_9MICC/155-294 AC A0A1Y1RMG1.1
#=GS A0A1Y4GKR9_9BACT/157-293 AC A0A1Y4GKR9.1
#=GS A0A7Y4LB84_9BURK/155-287 AC A0A7Y4LB84.1
#=GS A0A6S6XGQ4_9FIRM/157-293 AC A0A6S6XGQ4.1
#=GS A0A419T8E6_9FIRM/147-282 AC A0A419T8E6.1
#=GS A0A0V8JLM2_9BACI/150-288 AC A0A0V8JLM2.1
#=GS A0A0R1ZT34_9LACO/181-321 AC A0A0R1ZT34.1
#=GS A0A1V4I7R7_9FIRM/153-288 AC A0A1V4I7R7.1
#=GS C6JKY8_FUSVA/147-281 AC C6JKY8.1
#=GS A0A1I6BRX5_9BACI/150-288 AC A0A1I6BRX5.1
#=GS A0A1M7IV76_9FIRM/157-293 AC A0A1M7IV76.1
#=GS A0A3E2NAJ0_9CLOT/147-282 AC A0A3E2NAJ0.1
#=GS A0A1Y4GEU6_9BACT/157-293 AC A0A1Y4GEU6.1
#=GS R9JR57_9FIRM/147-282 AC R9JR57.1
#=GS R6AWC6_9BACT/163-298 AC R6AWC6.1
#=GS A0A1C0BUS6_9FIRM/156-287 AC A0A1C0BUS6.1
#=GS A0A172Q519_9STRE/155-286 AC A0A172Q519.1
#=GS V9H5A9_9CLOT/153-288 AC V9H5A9.1
#=GS R6MYT0_9CLOT/157-294 AC R6MYT0.1
#=GS A0A0D4CKH1_LIMMU/156-289 AC A0A0D4CKH1.1
#=GS A0A1G4FI67_9FIRM/123-247 AC A0A1G4FI67.1
#=GS E0NM75_9FIRM/147-281 AC E0NM75.1
A0A1K1PVB9_RUMFL/148-283 ..EKEILTEAFLSYPPVCRILSLLEDGK.......SMTKFEIGCQLGFVGEAGFTSIPQALILQGLNTA..TTT..DERN...KLLSDTEGTSDKYVRTICAWL..KSMR..WVMQVSKE.VTADLGYMTHTDIIN.QS........YQITLEGKKILKYATGTSK................
A0A174M7L2_9CLOT/147-281 .e-EETLTTAFLSYPPVCRVLSLLAAGD.......HLTKFEIGARLGFIGEAGFTSIPQHMILQGLSET..A-E..EDRA...KLLQDTEGTSDKYVRTICSWL..KQMG..WVEQAAKT.AAEDAGTRRYEGTIP.QS........YRLTLKGRTVFKHTTGVSK................
A0A1Q2MCL2_9BACT/143-285 kf--EIIKHSLLSYPPVTRILELLNVQYqns.qepSLTKYEIGRELGFKGEAGFTSYSQKTVVHALSCA..ESN..PERT...KIKNNWEGSSDKYARMISKWLchNQVG..WVQTARKK.ITVQIGEKKFTSQL-.KS........YQITLEGIKIFKLSRA---hsr.............
I3DCT9_9PAST/154-285 el--DILRTALLAYPPATQILSLLEENG......dYCTKYYLGSKLGFRGEKGFTSYNEDIMYDWLASS..S-A..VEKK...KIKSDIEGTSDKYARGICNWL..KNVG..FVQ--TKQ.VK-----RKFQNGQE.ESl.....qgYKITGRGIIALHRSIGHSS................
A0A8J6TVX0_9FIRM/165-298 ..EQEVFTQALLSYPPVYRVLEILSDQK.......PKTKFEIGAQLGFQGEMGFTSIPQEIFVYDYSTA..PAA..KQ-Q...DIKQNEEGDSDKYARTIANWL..SQMK..WVKPMYKD.VTETYLGTTYTITL-.RA........WQITRAGELALKKSKGGS-s...............
D9R4Y5_LACSW/147-282 .e-AFALKNAFLSYPPVCRVLSLLGEEG.......HLTKFEIGARLGFIGEAGFTSIPQYMILQGLTEA..EAR..EEKT...KLLQDTEGTSDKYVRTICSWL..LQMG..WVIKVSKE.VTASIGGRAYTGTIP.QS........YQLTLKGRTILKHVTGVSK................
A0A137SQ27_9FIRM/147-281 .d-KEILISAFLSYPPAVRILTLLESGD.......HLTKFELGKQLGGLGEAGFTSIPQDLYIQAIELA..SE-..TDKA...SIRSNTEGSADKYARMISGWL..SKVG..LVQRVEKE.VSTKIGNVEYMVNIG.HS........FRITLNGIKELKRARGLS-s...............
G8PBE9_PEDCP/181-320 ..ETEIFQQTFISYPYAVGFLKALQGTK.......GLNKFQLGERFGFAGEAGFTSFGEELYINGMIEA..YEN..NDQKlisQIRSNWESTSDKYIRGLANVL..KKLN..LVNIDAQV.IP-------YTNKQT.NEkeefaipiYKITGIGRQQLGIALGKSS................
A0A0F2J3F6_9BACT/144-283 ey--KIYEEALLSYPPVSRIINLIAQAGen...ndVLTKFEIGKSLGFIGEEGFTSISQNLFVKEISSA..L-K..EEKI...KIRSNWEGDSDKYARMICSWL..TQLKfpWIERVRKK.INIDFAGKKYSYTL-.QA........YTITRKGYEIRKMISGSSK................
A0A1Y1RMG1_9MICC/155-294 nd--EYLLQAICSYPPATRVMNILENAKndkgeyiPLSKFQIGSQLGFKGEPGYTSYSHELAEQALIES..TSP..KEQN...SLRQDFEGTSDKYARMIASWL..TKLR..LVQSVQTK.IPNSLGEKIST---Y.QR........YLLTAKGVQQIKRINGNSS................
A0A1Y4GKR9_9BACT/157-293 ..EKELLINAALAYPPVVRVLSLLSETEd.....tHLTKFEIGRQLGFIGEDGFTSMPQKVFVRSLANA..EA-..TERS...TMKANWEGSSDKYARMIAKWL..ENLG..LVMRVSKE.VTVWLGEKSYTDTIA.HS........YIITAPGITALRRSVGKSR................
A0A7Y4LB84_9BURK/155-287 .e-RQVLKKALLAYPPATQILTLLSQTPt.....qFRTKFALGEHLGFRGEKGFSSYNEELILEWLARE..TDP..AQKK...KIRTDVEGTSDKYARGICSWL..IKVG..LVEQDDFV.TKVN-----NTTVKF.PG........YRITGSGIHALKQSQGNSS................
A0A6S6XGQ4_9FIRM/157-293 ..ERELLTEAALAYPPAVRILDLLARDG......aHLTKFEIGQKLGFVGENGFTSLPQPVLIRSLAGT..ESP..KEKN...KMKTDWDGSSDKYARMIASWL..TKLG..LVEQVPKQ.VTVTAGGRTYTETIG.QA........YLITARGITMRNRALGKSR................
A0A419T8E6_9FIRM/147-282 .e-AQVLKTAFLSYPPVCRVMTLLGEEV.......HLTKFEIGARLGFIGEAGFTSIPQYMILEGLLEA..KTK..EDKT...KLLQDTEGTSDKYVRTICSWL..MQMG..WISKVPKE.VTSNAYGSLSSATIP.QS........YKLTLKGRTVLKHTTGVSR................
A0A0V8JLM2_9BACI/150-288 ..EKIILIEALSSYPPAIRILTLLENGE.......HLTKFDLGKQLGFSGESGFTSLPQNLFLDALANA..PSSppTIKN...SIRSDWEGSADKYARMISGWL..VKMG..LVAQGRKNfVVPTVGGSTRNEYIS.HA........FKITKDGLDVLRRAKGASK................
A0A0R1ZT34_9LACO/181-321 el--TILQLSFMSYPYAVAFLKSLEDSK......eGLNKFQLGESFGFAGEAGFTSFGEELYVSALTEA..YED..GDRArikKIKSNWESTSDKYIRGIASLL..SKLK..LISIVDQL.YSYESPKTGRTSHFSlPA........YKLTGYGRENLGIALGRSK................
A0A1V4I7R7_9FIRM/153-288 ..EKDILIKAILSYPPAVRVLDLLSNGD.......HLTKYDIGRMLGFSGEAGFTSLPQNILINALAIC..DNN..EECN...RMKSDWDGSSDKYARMIGSWL..SKLG..LVSKISKN.FNVIVDGVTKTVSIA.HA........FKITPEGLRQLRRARGVN-k...............
C6JKY8_FUSVA/147-281 ..ENEILVTAFLSYPPAVRILNLLESGD.......ILTKFEIGKQLGGLGEAGFTSIPQDLYIQAIET-..TND..STKS...KIRSNTEGSADKYARMICSWL..LKVG..LVQKVKKE.VKTIIGSIEYKTKIG.HS........YKITLNGLKELKRTKGVSS................
A0A1I6BRX5_9BACI/150-288 ..EKAILIEALSSYPPAIRILTLLEKGD.......HLTKFELGKQLGFNGESGFTSLPQDLFLDSLATApsSPS..NIKS...SIRSDWEGSADKYARMIAGWL..SKMG..LVTQPRKDfVVPTVGGHTRNEYIS.HS........YKITKEGLDVIRRAKGGSK................
A0A1M7IV76_9FIRM/157-293 ..ETELLTRAALSYPPAVRILRLLADED......aHLTKFELGKQLGFIGEGGFTSLPQSVLLRTLAIT..KDP..SDIN...KMKADWEGSSDKYARMIAKWL..AKLG..LVEAVPKT.FQVPIQSEVYPVTIG.QA........YQITAKGYMALNHTNGKSS................
A0A3E2NAJ0_9CLOT/147-282 ..EEEVLKTALLSYPPVCRVLSLLMKEG.......HMTKFEIGAKLGFIGEAGFTSIPQSMIVEGLGAA..HEK..EEKT...KLLQDTEGTSDKYVRTICSWL..ISVG..WVKKIPKE.VPGLYGSGENTEVIP.QS........YQLTLKGRTICNHITGGSR................
A0A1Y4GEU6_9BACT/157-293 ..EKDLLINAALAYPPAVRVLSLLSETEd.....tHLTKFEIGRQLGFIGEDGFTSMPQKVFVRSLANA..EA-..TERR...TMKANWEGSSDKYARMIAKWL..ENLG..LVTRVPKE.ITVWSGEKSYTDTIA.HS........YTITAAGITALRRSIGKSR................
R9JR57_9FIRM/147-282 .e-EDILKRAFLSYPPVCRIMLLLENSE.......PLTKFEIGEQLGFIGEAGFTSIPQHLILQGLSEI..SDA..KERN...KLLSDTEGTSDKYARTICSWL..KQLG..WVTQVNKT.VSTILNEEEYSDTIA.QS........YQITLKGLRMIKHIKGISK................
R6AWC6_9BACT/163-298 ..KNTILGRAYLSYPPVCRVLGLLASKG.......HLTKFEIGSQLGFTDEAGFTSFPQNIWVQAYEEA..SDD..KEKK...TLRSDTEGSSDKYARMICGWL..EAIG..WVNKKPKT.VKENIGAKSYTCIIT.SA........FEITDLGIANYNKALGKSK................
A0A1C0BUS6_9FIRM/156-287 ei--NILRNSLLSYPPATQVLNILESTT......rPVSKFYIGNQLGFSGEKGFTSYDESLMFDWFN-S..GTN..EEQK...KIKSDIEGTSDKYARMISTWL..EKVG..FVQKNSTV.VN-TINGKKA---GF.QE........FSITGRGSHALRKAHGSSK................
A0A172Q519_9STRE/155-286 ei--AILQQALLAYPPATQILSLLKEHG......gYCTKYYLGEKLGFRGEKGFTSYNEDIMFDWLGAA..-TQ..SERK...SIKSDIEGTSDKYARGICNWL..KNVG..LVE--TKT.VKRLLDNGMVD--GF.QG........YKINARGIHLLNQSQGSSK................
V9H5A9_9CLOT/153-288 .e-STILEEAMLSYPPVARVLTLLSNGE.......HLTKYEIGKKLGFVGEAGFTSLPLNVLIMTLATT..DEP..KEKN...KIKTDWDGSSDKYARMISGWL..VKLG..LLVQRPKL.VTVDFGGELYSETIG.HA........YMITDRGLKAVRRLLGIN-k...............
R6MYT0_9CLOT/157-294 ..EKELLTSAVLAYPPAVRILSLLGEGEg.....aHLTKFELGKQLGFVGEDGFTSLPQTVLVRSLASS..KDA..KEKN...KMKTDWDGSSDKYARMIAKWL..EKLG..LVKQEAKP.VTVTLAGRKYTESIG.QS........YVITGLGITALNRTLGKSR................
A0A0D4CKH1_LIMMU/156-289 ..ERTILREAMLSYPPASRVMSLLADAK......ePMTKFAIGSQLGFIGEKGFTSYDEDLMIEWLKSA..NDA..NEIT...KIRSDVEGTSDKYARMICSWL..AKLK..YVTKKSTK.YT-SMAGKQITGF--.QK........YALTGEGLHAIKQANGSSK................
A0A1G4FI67_9FIRM/123-247 ei--EIFRKGILTNPPVVRVLALLNENRks..grgAMTKYDVGAELGFVGDIGFTHYDAEFVVASNK--..---..----...-SFNDMEGDSDKWARTIISWL..KQVG..WVIDGEKF.NCYGKQLRSYTTKT-.--........-------------------dvdrvlqytvkstiky
E0NM75_9FIRM/147-281 .d-KEILISAFLSYPPAVRILTLLENGD.......HLTKFELGKQLGGLGEAGFTSIPQDLYIQAIELA..-AD..KDKA...SIRSNTEGSADKYARMISGWL..SKVG..LIQRIGKE.VSTKIGDVEYKVNIG.HS........FRITLNGIKELKRAMGLS-s...............
#=GC seq_cons ...p-ILppAhLSYPPssRlLsLLppsc.......aLTKFEIGppLGFhGEsGFTShPQplhlpuLspu..pss..p-+s...+l+oDhEGoSDKYARhIsuWL..pplG..hVppssKp.VssphusppaospIs.pu........YpITtcGlphL++upGtSp................
//
