#=GF ID DUF6409
#=GF AC PF19947.3
#=GF DE Family of unknown function (DUF6409)
#=GF AU Williams LS;0000-0001-5551-8526
#=GF AU Finn RD;0000-0001-8626-2148
#=GF AU Fragoso S;0000-0001-7018-3891
#=GF SE Manual
#=GF GA 27.20 27.20;
#=GF TC 29.30 29.10;
#=GF NC 26.60 26.50;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 75585367 --cpu 4 -E 1000 HMM pfamseq
#=GF TP Family
#=GF WK Domain_of_unknown_function
#=GF RN [1]
#=GF RM 16766657
#=GF RT Amplification of the entire kanamycin biosynthetic gene cluster
#=GF RT during empirical strain improvement of Streptomyces
#=GF RT kanamyceticus.
#=GF RA Yanai K, Murakami T, Bibb M;
#=GF RL Proc Natl Acad Sci U S A. 2006;103:9661-9666.
#=GF DR INTERPRO; IPR045638;
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC This entry represents a member of a biosynthetic gene cluster
#=GF CC (BGC). This BGC (BGC0000703) is described by MIBiG as an example
#=GF CC of the following biosynthetic class, saccharide, in particular
#=GF CC the kanamycin biosynthetic gene cluster from Streptomyces
#=GF CC kanamyceticus [1].
#=GF SQ 24
#=GS A0A0K2B1Y7_STRA7/1-109 AC A0A0K2B1Y7.1
#=GS A0A291W5E8_9ACTN/1-125 AC A0A291W5E8.1
#=GS A0A2U8UWH6_9CAUD/1-124 AC A0A2U8UWH6.1
#=GS G2GF05_9ACTN/1-121 AC G2GF05.1
#=GS A0A1V4A2W4_9ACTN/1-132 AC A0A1V4A2W4.1
#=GS A0A0E3M322_9CAUD/1-120 AC A0A0E3M322.1
#=GS A0A117RN63_9ACTN/1-132 AC A0A117RN63.1
#=GS A0A4S2T562_9ACTN/1-118 AC A0A4S2T562.1
#=GS A0A8J3CLD5_9PSEU/1-127 AC A0A8J3CLD5.1
#=GS A0A6B2RMR7_9ACTN/1-132 AC A0A6B2RMR7.1
#=GS A0A1G9F3S1_9ACTN/1-132 AC A0A1G9F3S1.1
#=GS M3C8I3_9ACTN/1-118 AC M3C8I3.1
#=GS A0A2U8UWR0_9CAUD/1-124 AC A0A2U8UWR0.1
#=GS A0A6N7L0W1_9ACTN/322-443 AC A0A6N7L0W1.1
#=GS A0A6I6SHM9_9ACTN/1-132 AC A0A6I6SHM9.1
#=GS A0A7V8SUB6_9ACTN/1-132 AC A0A7V8SUB6.1
#=GS A0A429NGD2_9ACTN/1-127 AC A0A429NGD2.1
#=GS A0A125RNY5_9CAUD/1-121 AC A0A125RNY5.1
#=GS A0A1Q5LKG9_9ACTN/1-132 AC A0A1Q5LKG9.1
#=GS A0A4R7M9U7_9ACTN/5-130 AC A0A4R7M9U7.1
#=GS A0A7H8NAS6_9ACTN/1-133 AC A0A7H8NAS6.1
#=GS A0A4D6E4K7_9CAUD/1-120 AC A0A4D6E4K7.1
#=GS A0A7X6HWZ7_9ACTN/1-132 AC A0A7X6HWZ7.1
#=GS A0A646KTB2_STRJU/1-134 AC A0A646KTB2.1
A0A0K2B1Y7_STRA7/1-109 .....mn-------AT..TNTI.EAGTIVRAPRVVATVSTGV.RKGLVVDTFG....---.DSAFLVWFYGAGDA.KT.ET.....R-..LVMKREMTEV.GSV.....GDLSERMLSRLLGGMTEDG.PARAMGFKIHRLRAQ----------lrsarr.........................................
A0A291W5E8_9ACTN/1-125 .......MTTTRQNLT..VKDF.PAATLVLAGPWRRAQQLDS.RPAIVVGPFG....---.SMTLLVWYFTVGVP.EP.GD.....TV.qAMFPNELTPL.DDTlttmhPDAFRDIVRSVHRGRFAYG.DGGVLQTAVLRAWLQRSGLAYAAPE...............................................
A0A2U8UWH6_9CAUD/1-124 ..mtata--TATATAT..AAKF.ETGDVVWGRPVRQGKAQAR.RKGLVLGLFA....TDP.NQ-LVVWWFGQGAA.GM.DT.....TT..LAFARELTKS.GDI.....FDMGAVQAAKLARGCYRYE.RAHSVGRMLERHARRMKSLGASFPA...............................................
G2GF05_9ACTN/1-121 .......MTATATITS..QDVL.EAGTVVRCPRYVEGEDTGP.RKAVVLGLFR...eGDP.TGGYRVYFYTLGAA.GFaEH.....TV.gLAFRREMHPL.GTV.....EDMSERTLVRIWRGLGDFP.GSSTARYRVQRMGLR----------lrnarl.........................................
A0A1V4A2W4_9ACTN/1-132 .......MLTTDQKPT..VQDF.PTATLVIAGPWFRGHQLDT.RPAVVVGPFGgpefADE.ATMSVVWYFTLGAP.QP.GD.....SV.qAMFPHELTPL.DDTlttmhQDTFRDIVRDLRRGRFAYD.DGDALRAAVRQAWRERTGLADADPA...............................................
A0A0E3M322_9CAUD/1-120 .......MTATA-TAT..AAKF.ETGDVVWGRPVRQGKAQAR.RKGIVLGLFA....TDP.NQ-LVVWWFGQGAA.GM.DT.....TT..LAFARELTKS.GDI.....FDMGAVQAAKLARGCYRYE.RAHSVGRMLERHARRMKSLGASFPA...............................................
A0A117RN63_9ACTN/1-132 .......MVTTDKKPT..VQDF.PTATLVLAGPWFRGHQLDT.RPAVVVGPFGgpefADE.ATMAVVWFFTLGAP.EP.GD.....SV.qAMFPHELTPL.DDTlttmnQDVFRDIVCGLRRGRFAYD.DGDALRAAVRQAWRERTGLADADPA...............................................
A0A4S2T562_9ACTN/1-118 .......MTVAIASAT..QTVL.EPGTVVRTPRYDEGKNTGV.RRAIVLGLWN...eEEP.KGGYKVWFYTLGAP.SF.AP.....RTlgMAFANEPIVV.STV.....EEMSER-------------.-------------------------tllrlyvqlrdynlrslagygavehrirkag................
A0A8J3CLD5_9PSEU/1-127 .....mq------TTT..VPQLpEKGSVITARPWQDGQRQAKaVRGLVVQHVH...tENPtDAYIVVWFPALGAP.AV.GDpdttpTV.qAILPREIDT-.---.....-------------------.-------------------------vatldqlpatwvittertarhtrrtggydiglaagvplerlartyra
A0A6B2RMR7_9ACTN/1-132 .......MLTTDQKPT..VRDF.PTATLVIAGPWHRGHQQDT.RPAVVVGPFGgpefADE.ATMSVVWFFTLGAP.EP.GD.....SV.qAMHPHELTPL.DDTlttmnPDVFRDIVCGLRRGRFAYD.DGDALRTAVRQAWRERTGLADADPA...............................................
A0A1G9F3S1_9ACTN/1-132 .......MLTTDKKPT..VQDF.PTATLVIGGPWFRGHQLDT.RPAVVVGPFGgpefADE.ATMSVVWFFTLGAP.QP.GD.....SV.qAMFPHELTPL.DDTlttmhGDTFRDIVRSLRRGRFAYD.DGDALRAAVRQAWRERTGLTDADPA...............................................
M3C8I3_9ACTN/1-118 .....mt-------AT..HQTY.TAGDVVTGRPVRHMEPQAR.RKGIVLRLFG....TDP.SAGYIVWWYGMGPA.SM.KT.....TS..LMFPRELTPA.GTL.....DDLSEAVLRRIERGCGTYG.DAHGVGLAASRRRVQRR--------narreaaqa......................................
A0A2U8UWR0_9CAUD/1-124 ..mtata--TATATAT..AAKF.ETGDVVWGRPVRQGKAQAR.RKGLVLGLFA....TDP.NQ-LVVWWFGQGAA.GM.DT.....TT..LAFARELTKS.GDI.....FDMGAVQAAKLARGCYRYE.RAHSVGRMLERHARRMKSLGASFPA...............................................
A0A6N7L0W1_9ACTN/322-443 .....vm--NTATAP-..VAAL.AVGTVLYCKRVKQAEVLGT.GKAIVLGRLG....ADE.FAPYIVWFYTEGPAvQG.DD.....STvsLAFPREVERTvGRF.....EDFSLTTLRAIAKGSEDFS.GAVEVWRIARAELRRRA--------ehraa..........................................
A0A6I6SHM9_9ACTN/1-132 .......MLTTDQKPT..VQDF.PTATLVIGGPWFRGHQLDT.RPAVVVGPFGgpefADE.ATMSVVWFFTLGAP.QP.GD.....SV.qAMFPHELTPL.DDTlttmhQDTFRDIVRSLRRGRFAYD.DGDALRAAVRQAWRERAGLADADPA...............................................
A0A7V8SUB6_9ACTN/1-132 .......MLTTDQKPT..VQDF.PTATLVIAGPWFRGHQLDT.RPAVVVGPFGgpefADE.ATMSVVWYFTLGAP.EP.GD.....SV.qAMFPHELTTL.DDTlatmhQDTFRDIVRNLRRGRFAYD.DGDALRAAVREAWRERTGLADADPA...............................................
A0A429NGD2_9ACTN/1-127 ....mkp---SS--PT..AEDF.PTATLVLAGPWSRGRRLES.RPGVVVGPFG...vAEE.AVCVLVWYFTLGAP.EP.GT.....TV.qAMLPDELTPL.DDTlttmhEDVFRDLVRSLRRGRIAHG.DGEALYAAVCQAWLRRIGLADAAS-g..............................................
A0A125RNY5_9CAUD/1-121 .......MQNATATTG..-RRF.ETGDVVFGRPIFEGKIQPR.RKGVVLGAWT....EGD.TKRVVVWWYGLGAA.SG.DT.....SS..LMWNDELTPA.GDI.....FDTSARMAAKLFEGAYTYP.RARFVCQLLRNHARRMESLGAKHPV...............................................
A0A1Q5LKG9_9ACTN/1-132 .......MLTTDQKPT..VQDF.PTATLVIAGPWFRGHQLDT.RPAVVVGPFGgpefADE.ATMSVVWFFTLGAP.QP.GD.....SV.qAMFPHELTPL.DDTlttmhQDTFRDIVRSLRRGRFAYD.DGDALRAAVRQAWRERAGLADADPA...............................................
A0A4R7M9U7_9ACTN/5-130 anrterp------VPT..VAEL.VPGTLVTVRPLPGFDRIPA.SCGVVLDAWG....---.-DCTLVWFWGLGLP.VA.GK.....SV.hAIFPREITVL.TTTletvpSGAFESIAAG---------.-------------------------ldtlkgqswypselqplierveevrarraaqlaer............
A0A7H8NAS6_9ACTN/1-133 .......MLTASQKPT..VQDF.PAATLVIGGPWFRSRQLDT.RPAVVVGPFGgpefADK.ATMAVVWFFTLGAP.QP.GD.....TL.qAMFPHELTPL.DDTlttmhQDAFRDIVRSLRRGRFAYDdDGDALRAAVRQAWRERTGLADADPA...............................................
A0A4D6E4K7_9CAUD/1-120 .......MTNTTATKV.tAAEL.VAGTIVWGRPVREGKAQSR.RKGLVLGSFG....---.DSAVIVWWYGEGAA.SG.ST.....TT..MAFPRELTRD.GDM.....FDLSARVARKLAKGCYGFE.RAASVGWSLTRHAARMASLGASQPV...............................................
A0A7X6HWZ7_9ACTN/1-132 .......MLTTPHKRT..VQDF.PTATLALAAPWFRAHQLDT.RPALVVGPFGgpecADE.ATMAVVWFFTMGAP.EP.GG.....SL.qAMFPHELTPI.DDTlttmhEDTFRDIVRSLRRGRFAYD.DGGALCDAVRRAWRERAGLADADS-a..............................................
A0A646KTB2_STRJU/1-134 .......MTTTYQTPVttAQDF.PAATFVLGGPWERGNQIDP.RPAVVVGSFGgrerAAE.TSPLVVWFFSGGAP.EP.GP.....TV.qAMFPHELTPI.GDTlttmdGQAFRVLAASLHRGRFAYD.DGDELRAAVRQAGRERTGLTDADPA...............................................
#=GC seq_cons .......MhsostpsT..spcF.ssuTlVhusPahcG+phss.R.ulVlGsFG....sD..sshhVVWaFolGAP.ps.us.....ol.phhFP+ELTsl.sDs.....tDhhccllppLtRGpasYs.cucultttlcpthRchtuLusu.P................................................
//
