GenomeNet

Database: Pfam
Entry: Flavi_NS2A
LinkDB: Flavi_NS2A
Original site: Flavi_NS2A 
#=GF ID   Flavi_NS2A
#=GF AC   PF01005.20
#=GF DE   Flavivirus non-structural protein NS2A
#=GF AU   Finn RD;0000-0001-8626-2148
#=GF AU   Bateman A;0000-0002-6982-4660
#=GF SE   Pfam-B_156 (release 3.0)
#=GF GA   25.10 25.10;
#=GF TC   96.10 94.50;
#=GF NC   23.60 23.20;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF RN   [1]
#=GF RM   9636360
#=GF RT   Subcellular localization and some biochemical properties of the
#=GF RT   flavivirus Kunjin nonstructural proteins NS2A and NS4A. 
#=GF RA   Mackenzie JM, Khromykh AA, Jones MK, Westaway EG; 
#=GF RL   Virology 1998;245:203-215.
#=GF RN   [2]
#=GF RM   7474145
#=GF RT   Evidence that flavivirus NS1-NS2A cleavage is mediated by a
#=GF RT   membrane-bound host protease in the endoplasmic reticulum. 
#=GF RA   Falgout B, Markoff L; 
#=GF RL   J Virol 1995;69:7232-7243.
#=GF DR   INTERPRO; IPR000752;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   NS2A is a hydrophobic protein about 25 kDa is size.  NS2A is 
#=GF CC   cleaved from NS1 by a membrane bound host protease [2].  NS2A
#=GF CC   has been found to associate with the dsRNA within the vesicle
#=GF CC   packages.  It has also been found that NS2A associates with the 
#=GF CC   known replicase components and so NS2A has been postulated to be
#=GF CC   part of this replicase complex [1]. 
#=GF SQ   3
#=GS POLG_DEN1W/1138-1326  AC P17763.2
#=GS POLG_YEFV1/1139-1323  AC P03314.1
#=GS POLG_ZIKV/1154-1338   AC Q32ZE1.1
POLG_DEN1W/1138-1326             GLLCISIMIEEVMRSRWSRKMLMTGTLAVFLLLTMGQLTWNDLIRLCIMVG------ANASDKMgmgttYLALMATFRMRPMFAVGLLFRRLTS-REVLLLTVGLSLVasvelpnsleELGDGLAMGimmlklltdfqshQLWATLLSLTFVKTTFSLHYAWKTMAMILSIVSLF-PLCLSTT------------------------------sqkttwlpvllgsl................
POLG_YEFV1/1139-1323             GLVSMMIAMEVVLRKRQGPKQMLVGGVVLLGAMLVGQVTLLDLLKLTVAVGLHFHEMNNGGDAM.....YMALIAAFSIRPGLLIGFGLRTLWSPRERLVLTLGAAMV..........EIALGGVMG.............GLWKYLNAVSLCILTINAVASRKASNTILPLMALLTPVTMAEVRLAAMFFCAVVIIGVLHQNFKDTSMQKTIP..............................
POLG_ZIKV/1154-1338              GVLVILLMVQEGLKKRMTTKIIMSTSMAVLVVMILGGFSMSDLAKLVILMGATFAEMNTGGDVA.....HLALVAAFKVRPALLVSFIFRANWTPRESMLLALASCLL..........QTAISALEG.............DLMVLINGFALAWLAIRAMAVPRTDNIALPILAALTPLARGTL------------------------------lvawraglatcggimllslkgkgsvkknlp
#=GC seq_cons                    GLLsIhIMlEEVLRKRhosKhlMoGolAVLllMllGQlThsDLlKLsIhVGhpFtEMNNGGDsM.....YLALlAAF+lRPuLLVGFlFRsLWSPREsLLLTLGuuLV..........ElAlGulMG.............sLWshLNuloLshLTIsAhAuhKTsNhILPIlALLTPLshuTl................................thphh.shhGul................
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