GenomeNet

Database: Pfam
Entry: PF00469
LinkDB: PF00469
Original site: PF00469 
#=GF ID   F-protein
#=GF AC   PF00469.21
#=GF DE   Negative factor, (F-Protein) or Nef
#=GF AU   Finn RD;0000-0001-8626-2148
#=GF SE   Pfam-B_128 (release 1.0)
#=GF GA   20.60 15.00;
#=GF TC   27.00 46.30;
#=GF NC   19.30 14.90;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF WK   Nef_(protein)
#=GF RN   [1]
#=GF RM   9351809
#=GF RT   The crystal structure of HIV-1 Nef protein bound to the Fyn
#=GF RT   kinase SH3 domain suggests a role for this complex in altered T
#=GF RT   cell receptor signalling 
#=GF RA   Arold S, Franken P, Strub M-P, Hoh F, Benichou S, Benarous R,
#=GF RA   Dumas C; 
#=GF RL   Structure 1997;5:1361-1372.
#=GF DR   INTERPRO; IPR001558;
#=GF DR   SCOP; 1avv; fa;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   Nef protein accelerates virulent progression of AIDS by its
#=GF CC   interaction with cellular proteins involved in signal
#=GF CC   transduction and host cell activation. Nef has been shown to 
#=GF CC   bind specifically to a subset of the Src kinase family.
#=GF SQ   27
#=GS A0A1B4WRK5_SIV/2-225  AC A0A1B4WRK5.1
#=GS Q87114_SIVSA/2-223    AC Q87114.1
#=GS NEF_HV2SB/2-235       AC P12447.3
#=GS NEF_HV2UC/2-217       AC Q76639.3
#=GS Q88019_SIV/2-92       AC Q88019.1
#=GS O11408_9PLVG/2-235    AC O11408.1
#=GS Q6R786_9HIV2/2-235    AC Q6R786.1
#=GS C7TQ74_9HIV1/2-214    AC C7TQ74.1
#=GS NEF_HV2BE/2-235       AC P18092.3
#=GS NEF_SIVEK/2-205       AC Q1A242.3
#=GS Q9WLL7_9HIV1/2-218    AC Q9WLL7.1
#=GS NEF_HV1ET/2-206       AC Q75009.3
#=GS NEF_SIVTN/7-195       AC Q8AIH4.3
#=GS C7TQ92_9HIV1/2-208    AC C7TQ92.1
#=GS NEF_HV2G1/2-234       AC P18038.3
#=GS NEF_SIVSP/2-233       AC P19501.3
#=GS B3CKG6_SIV/2-212      AC B3CKG6.1
#=GS NEF_HV1B1/2-205       AC P03404.4
#=GS NEF_HV1B1/2-205       DR PDB; 1ZEC A; 1-25;
#=GS NEF_HV1B1/2-205       DR PDB; 6CRI N; 6-204;
#=GS NEF_HV1B1/2-205       DR PDB; 6CRI Y; 6-204;
#=GS NEF_HV1B1/2-205       DR PDB; 6CRI Z; 6-204;
#=GS Q8AII3_SIV/2-181      AC Q8AII3.1
#=GS W8E7N7_9PLVG/2-204    AC W8E7N7.1
#=GS NEF_HV1AN/2-212       AC Q77378.3
#=GS NEF_HV1AN/2-212       DR PDB; 3WLB C; 1-10;
#=GS NEF_HV1H2/2-205       AC P04601.5
#=GS NEF_HV1H2/2-205       DR PDB; 4NEE K; 73-203;
#=GS NEF_HV1H2/2-205       DR PDB; 4NEE H; 74-203;
#=GS NEF_HV1H2/2-205       DR PDB; 3RL2 C; 1-10;
#=GS NEF_HV1H2/2-205       DR PDB; 4NEE C; 72-203;
#=GS NEF_HV1H2/2-205       DR PDB; 5HGH C; 1-10;
#=GS NEF_HV1H2/2-205       DR PDB; 5HGD F; 1-10;
#=GS NEF_HV1H2/2-205       DR PDB; 5HGD C; 1-10;
#=GS NEF_HV1H2/2-205       DR PDB; 4NEE E; 72-203;
#=GS NEF_HV2ST/2-234       AC P20868.3
#=GS NEF_HV1EL/2-206       AC P04604.3
#=GS A8E116_9HIV1/2-206    AC A8E116.1
#=GS NEF_SIVMB/2-205       AC Q1A260.3
#=GS P89910_SIVTA/2-216    AC P89910.1
A0A1B4WRK5_SIV/2-225             ....................GSGNSKPQHK.KQLALWRALHKTQPTRYGLLADPLIGQSSTLQEECGRS.VRESLIKKRptkGRMTPEG................RRLQEGDTW.EEWSDDE.EEIGFPVKPRVPLRQMTYKLAVDFSHFLKEKGGLEGIYYSERRDKILNLYALNEWGILDDWNAWTPGPGIRYPRCFGFCFKLVPVELHEEAQ....NCERHCLVHPAQMIEDPDgiNHGEVLVWKFDPMLAVEFKAGIHF------sdmha..
Q87114_SIVSA/2-223               ....................GGKSSKQQQR.HSLWLWSKLRQAPVIQYDMLADPLLGQSSHIQEECAKS.LRDGLIRQG...DSSRTEEg.............vkMKHQGRQPS.W-YDEDE.EEVGFPVRPCLPLRAMTYKLAIDFGHFLKEKGGLEGIYYSERRKKILDLYALNEWGIVDGWQNYTDGPGTRYPKCFGWCFKLVPVDLSEEAK....NSENHCLLHPAQVAYEDD..AWKETLVWKFDPLLAVDYVAWRLHPEQV--ps.....
NEF_HV2SB/2-235                  ....................GASGSKKRSR.PSRGLQERLLRARGGACGGLWDESEGGYSQFHEGSGRE.QKLPSCEGQ...RYQQGDFmntpwrtpatekekesYRQQNMD--.DVDSDDD.DLVGVSDTSRVPLRAMTYRMAVDMSDLIKDKGGLEGMYYSERRHRILDIYLEKEEGIIPDWQNYTHGLGVRYPMFFGWLWKLVPVTVPQEGE....DTETLCLMHSAQVSRFDD..PHGETLVWKFDPMLAHEYTTFILYPEEFGH.......
NEF_HV2UC/2-217                  ....................GSAGSKKQSK.QQRGLRERLLRTQEEPYGKLSEGQRKQSSRSPGGSDKD.LNSPSCEGR...NAPRAEG................GGQQDTD--.--DSDED.NEVGVYVRPNRPLRSMTYKMAIDMSHFIKEKGGLEGIYYSERRHRILDTYLENEEGIVSGWQNYTYGPGIRYPRTFGWLWKLVPVDIPEEER....GAETSCLVHPAQISSWDD..IHGETLAWRFDPLLAHDYVAFNRYPEEFG-y......
Q88019_SIV/2-92                  ....................GGAISMRRSR.PSGDLRQRLLRARGETYGRLLGEVEDGYSQSPGGLDKG.LSSLSCEGQ...KYNQGQYmntpwrnpaeereklaYRKQNMDD-.-I-----.--------------------------------------------------------------------------------------------....------------------..------------------------------de.....
O11408_9PLVG/2-235               ....................GGAISMRRSR.PSGDLRQRLLRARGETYGRLLGEVEDGYSQSPGGLDKG.LSSLSCEGQ...KYNQGQYmntpwrnpaeereklaYRKQNMD--.DIDEEDD.DLVGVSVRPKVPLRTMSYKLAIDMSHFIKEKGGLEGIYYSARRHRILDIYLEKEEGIIPDWQDYTSGPGIRYPKTFGWLWKLVPVNVSDEAQ....EDEEHYLMHPAQTSQWDD..PWGEVLAWKFDPTLAYTYEAYVRYPEEFG-s......
Q6R786_9HIV2/2-235               ....................GASGSKERSR.PSQGLRERLLRARGETCGGHCSGSGGEYLQSQEESGRE.QRSPSYEGQ...RYQQGDFmntpwrtpakegekelYKQQNRD--.DVDSDDD.DLVGVSVTPRVPLRELTHRLAIDVSHFIKEKGGLEGMYYSERRHRILDIYLEKEEGIIADWQNYTHGPGIRYPMFFGWLWKLVPVDVTRQEE....DDGTHCLLHPAQTSRFDD..PHGETLIWKFDPTLAHDYKAFILHPEEFGH.......
C7TQ74_9HIV1/2-214               ....................GGKWSKSSIV.GWPEVRERMRRAR---PARE----RMRSSSAAE--GVG.AASKDLEKY...GALTSSN................TPSTNPDCA.WVAAQEEeEEVGFPVRPQVPLRPMTYKAAFDLGYFLKEKGGLDGLIYSQQRKDILDLWVYNTQGFFPDWQNYTPGPGTRYPLTFGWCYKLVPVDPDEVEEa..nKEENNSLLHPMSLHGM-D..TEGEVLKWQFDSQLALKHRARELHPEFYKD.......
NEF_HV2BE/2-235                  ....................GASGSKKLSK.HSRGLRERLLRARGDGYGKQRDASGGEYSQFQEESGRE.QNSPSCEGQ...QYQQGEYmnspwrnpaterqkdlYRQQNMD--.DVDSDDD.DLIGVPVTPRVPRREMTYKLAIDMSHFIKEKGGLQGMFYSRRRHRILDIYLEKEEGIIPDWQNYTHGPGVRYPMYFGWLWKLVSVELSQEAE....EDEANCLVHPAQTSRHDD..EHGETLVWQFDSMLAYNYKAFTLYPEEFGH.......
NEF_SIVEK/2-205                  ....................GKVWSKSSLV.GWPQVIDRMRRQ--QDP-----------AEGVG-----.AVSQDLANR...GAITIRN................TKENNQALA.WLEEQKE.EAVGFPVCPQVPLRPMTYKEAFDLSFFLREKGGLEGLVWSTKRQEILDLWVYHTQGIFPDWQNYTPGPGVRYPLTFGWCFKLVPLSPEEVEEa..nQGDNNVLLHPMCQHGMED..PDKEVLVWRFDSSLARVHRARELHPEFYQ-n......
Q9WLL7_9HIV1/2-218               ....................GGKWSKSSIV.GWPEIRERMRRARAEPE-RMRRAQAEPAAAGVG-----.AVSQDLDKH...GAITINN................TAATNPDKT.WLEAQEEeEEVGFPVRPQVPLRPMTFKGALDLSHFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWDNYTPGPGERFPLCFGWCFKLVPVDPQEVEEa..tEGENTCLLHPISQHGMED..EEREVLKWKFDSRLAYKHVARELHPEFYKD.......
NEF_HV1ET/2-206                  ....................GGTMSKCSPV.GWPAIRERIRRA---APAAE----------GVG-----.AASRDLDKY...GALTSSN................TPANNPDCA.WLEAQEEeEEVGFPVRPQVPLRPMTYKAAFDLSLFLKEKGGLEGLIYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPSEVEEi..nEGENNCLLHPASLHGMED..EDREVLKWKFDSHLARRHMARELHPEYYKD.......
NEF_SIVTN/7-195                  rwpgarkaiedlhntssepv----------.-------------------------------------G.QASQDLQNK...GGLTTNT................LG-TSADVLeYSADHTE.EEVGFPVRPAVPMRPMTEKLAIDLSWFLKEKGGLDGLFFSPKRAAILDTWMYNTQGVFPDWQNYTPGPGIRYPLCRGWLFKLVPVDPPE---....DDEKNILLHPACSHGTTD..PDGETLIWRFDSSLARRHIARERYPEYFK-.......
C7TQ92_9HIV1/2-208               ....................GGKWSKSSIV.GWPAVRERMRRAIPAPAA-----------EGVG-----.AVSRDLEKH...GAITSSN................TPDTNPDCA.WVQAQEEeEEVGFPVRPQVPVRPMTYKAAFDLGFFLKEKGGLDGLIYSQKRKDILDLWVYNTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPTEVEEa..nKGENNSLLHPLSLHGMDD..SEGEVLMWKFDSSLARRHVARELHPEFYKD.......
NEF_HV2G1/2-234                  ....................GASGSKKHSK.HSQRLRERLLRAHGGGYVQQCNASGGEYSQSQEGSGKG.QKSPSCEGQ...QYRQGDFmntpwrtpaiegqkklYKQQNMD--.DIDSSDD.DLVGVPVTPRVPLRAMTYKLAVDMSHFIKKR-GLDGMFYSRDRHRILDLYLEKEEGIIPDWQNYTHGPGVRYPMCFGWLWKLVPVDVSQEAE....DDETNYLTHPAQTSRHDD..EHGETLLWRFDPTLAYDYKAFILHPEEFGH.......
NEF_SIVSP/2-233                  ....................GGVTSKKQRR.RGGNLYERLLQARGETYGRLWEGLEGEYSQSQDASGKG.LSSLSCEPQ...KYCEGQFmntpwrnpaterakldYRQQNMD--.--DVDSA.DLVGCPVSPRVPVRIMTYKLAIDMSHFIKEKGGLEGIYYSDRRHKILDLYLEKEEGIIPDWQNYTAGPGIRYPMFFGWLWKLVPVNVSDEAQ....EDETHYLMHPAQTSQWDD..PWGEVLAWKFDPKLAYNYKAFVEHPEEFG-s......
B3CKG6_SIV/2-212                 ................ggvf----SKDSVEqRMLRYRRRLLRV-------GRKERRRRYTRLAAERQAEaAASGELLPD...-SAQT--................LIRAEEDSS.KEDSSDD.DAIGFPVRPQVPLRQATFKILVDLSHLFKEKGGLEDIFWSPRREEIVNLYAYHEWGIVEGWQAYTDGPGVRYPKTFGWCWKLVPVHFSEESPnsdqGFEKNLLLHPAV-AESED..PWKEYLVWKFDPRLACDFVA----------grladg.
NEF_HV1B1/2-205                  ....................GGKWSKSSVI.GWPAVRERMRRA---EPAAD----------GVG-----.AASRDLEKH...GAITSSN................TAANNAACA.WLEAQEE.EKVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEPDKVEEa..nKGENTSLLHPVSLHGMDD..PEREVLEWRFDSRLAFHHVARELHPEYFKN.......
#=GR NEF_HV1B1/2-205       SS    ....................XXSSTHTTST.TTTHHHHHHHHS--------------------------.-X-------...-------................---------.-------.---SEE--TT---B---HHHHHHHHHHHHHH-S-TT-EE-HHHHHHHHHHHHHHT-B-TTSS-B--SSS-B-B-STT--EEEEE---------..----SSBSS----------..-----EEEEE-GGGTTS-HHHHH-STTT-X.......
Q8AII3_SIV/2-181                 ....................GGKSSKQQEE.KYLKYYKAMRRG----YGAE--GTNGDYQQLHA-----.-SEPLLGAL...STSQEEF................DREQKSS--.--STDEE.EETGFPVYPQCPVREPTYKDLVDMPHFLKEKGGLEGIWHSKRREEILDLYAQNEWGFIPTWQSYTDGPGIRYPKTFRFLFKLCPVAVPPDQE....NNECNKLLQSSQLGIQEE..------------------------------slgrea.
W8E7N7_9PLVG/2-204               ....................GGKWSKCGLG.EWPRVRERMRAE----PAAD----------GV-----G.AASRDLERH...GALTSSN................TTATNEACA.WLEAQED.EEVGFPVRPQVPLRPMTFKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVHHTQGYFPDWQNYTPGPGVRFPLTFGWCFKLVPVDPQTVEEn..nEGENNCLLHPMSQHGVDD..ADREVLMWKFDSSLARRHIARELHPEYYKD.......
NEF_HV1AN/2-212                  ....................GNALRKGKFE.GWAAVRERMRRTRTFPES-------EPCAPGVG-----.QISRELAAR...GGIPSSH................TPQNNAALA.FLESHQE.EEVGFPVAPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSHKRAEILDLWVYNTQGFFPDWQNYTPGPGTRFPLTFGWLFKLVPVSEEEAERl.gnTCERANLLHPACAHGFED..THKEILMWKFDRSLGNTHVAMITHPELFQ-k......
#=GR NEF_HV1AN/2-212       SS    ....................XXXXXXXXXX.XXXXXXXXXXXXXXXXXX-------XXXXXXXX-----.XXXXXXXXX...XXXXXXX................XXXXXXXXX.XXXXXXX.XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----S-----XXXXXXXXXXXXXXXXXXXXX.XXXXXXXXXXXXXXXXXXXX..XXXXXXXXXXXXXXXXXXXXXXXXXXXXX-X......
NEF_HV1H2/2-205                  ....................GGKWSKSSVI.GWPTVRERMRRA---EPAAD----------RVG-----.AASRDLEKH...GAITSSN................TAATNAACA.WLEAQEE.EEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKIEEa..nKGENTSLLHPVSLHGMDD..PEREVLEWRFDSRLAFHHVARELHPEYFKN.......
#=GR NEF_HV1H2/2-205       SS    ....................XXXXXXXXXX.XXXXXXXXXXXX---XXXXX----------XXX-----.XXXXXXXXX...XXXXXXX................XXXXXXXXX.XXXXXXX.XXXXXXXX--S-SB---HHHHHHHHHHHHHTS--TT-B--HHHHHHHHHHHHHHH-B-TTS--B--SSS-B-BSSTT--EEEEE--HHHHHHH..T-SS-SBTTSGGGGTSSSS..TT---EEEEE-SHHHHS-HHHHH-GGG-XX.......
NEF_HV2ST/2-234                  ....................GASGSKKRSE.PSRGLRERLLQTPGEASGGHWDKLGGEYLQSQEGSGRG.QKSPSCEGR...RYQQGDFmntpwra.paegekgsYKQQNMD--.DVDSDDD.DLVGVPVTPRVPLREMTYRLARDMSHLIKEKGGLEGLYYSDRRRRVLDIYLEKEEGIIGDWQNYTHGPGVRYPKFFGWLWKLVPVDVPQEGD....DSETHCLVHPAQTSRFDD..PHGETLVWRFDPTLAFSYEAFIRYPEEFG-y......
NEF_HV1EL/2-206                  ....................GGKWSKSSIV.GWPAIRERIRRT---NPAAD----------GVG-----.AVSRDLEKH...GAITSSN................TASTNADCA.WLEAQEEsDEVGFPVRPQVPLRPMTYKEALDLSHFLKEKGGLEGLIWSKKRQEILDLWVYNTQGIFPDWQNYTPGPGIRYPLTFGWCYELVPVDPQEVEEd..tEGETNSLLHPICQHGMED..PERQVLKWRFNSRLAFEHKAREMHPEFYK-n......
A8E116_9HIV1/2-206               ....................GGKWSKSRIG.GWPAIRERMRQT---PPAAG----------GVG-----.AVSQDLANR...GAVTSNN................TVVDNPDRA.WLEAQEEgEEVGFPVRPQVPTRPMTYKGALDLSHFLKEKGGLDGLIYSQQRQDILDLWVYNTQGYFPDWQNYTPGPGERFPLTFGWCFKLVPVDPQEVEAa..nEGENNSLLHPMCQHGMED..KDREVLIWRFDSRLAFRHRAREQHPEYYKD.......
NEF_SIVMB/2-205                  ....................GGKWSKSSLV.GWPEVRDRLRRT---QTAAE----------GVG-----.PVSRDLARH...GAITSRN................TSQTNETLA.WLEEVQN.EEVGFPVRPQVPLRPMTFKGAFDLSHFLKEKGGLEGLVYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPLEPEEVERa..nEGDNNILLHPICQHGQED..EAREVLVWTFDSRLALKHRARELHPEYYKD.......
P89910_SIVTA/2-216               ....................GGSNSKREQQ.GLLRLWRALRKAPVVRYGMLADPLIGQSSNIQEECDKN.WNGGSTR-R...GKSTPEG................RKLAADDTW.DDWEPEE.EEVGFPVRPRVPLRQMTYKLAVDFSHFLKEKGGLEGIYWSPKREQILNLYALNEWGIIDDWQAYSPGPGTRKPRCFGFCFELVPVDVSQEAQ....-DERHCLLHPAQIEWESD..PWKETLVWKFNPLLAVQYNP----------dsfkdmh
#=GC SS_cons                     ....................XXSSTHTTST.TTTHHHHHHHHS---XXXXX----------XXX-----.XXXXXXXXX...XXXXXXX................XXXXXXXXX.XXXXXXX.XXXSEEXXTTS-SB---HHHHHHHHHHHHHCSS-TT-EE-HHHHHHHHHHHHHHC-B-TTSS-B--SSS-B-BSSTT--EEEEE--HHHHHHH..T-SSSSBTTSGGGGTSSSS..TT---EEEEE-CHHCCS-HHHHH-CCCTXX.......
#=GC seq_cons                    ....................GuphSKpp.t.s..tlRERhhRs...t.ut..........pu.t......tsStsl-tp...sshpupt................htppN.D....l-up--.-cVGFPVpPpVPLRsMTYKhAlDhSHFLKEKGGLEGlhYSp+RpcILDLalhpppGhhPDWQNYTsGPGlRYPhsFGWhaKLVPV-ssc.tc....csEsssLLHPsphpth-D..sctEsLhW+FDspLAhcahAh.haPE.at........
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