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Database: PROSITE(DOC)
Entry: PDOC00753
LinkDB: PDOC00753
Original site: PDOC00753 
{PDOC00753}
{PS00977; FAD_G3PDH_1}
{PS00978; FAD_G3PDH_2}
{BEGIN}
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* FAD-dependent glycerol-3-phosphate dehydrogenase signatures *
***************************************************************

FAD-dependent glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) (GPD) catalyzes
the conversion  of  glycerol-3-phosphate  into  dihydroxyacetone phosphate. In
bacteria [1]  it  is  associated  with  the utilization of glycerol coupled to
respiration. In  Escherichia coli, two isozymes are known: one expressed under
anaerobic conditions (gene glpA) and one in aerobic conditions (gene glpD). In
eukaryotes, a mitochondrial form of GPD participates in the glycerol phosphate
shuttle in conjunction with  an NAD-dependent cytoplasmic GPD (EC 1.1.1.8) [2,
3].

These enzymes  are  proteins  of  about  60  to 70 Kd which contain a probable
FAD-binding domain in their N-terminal extremity. The mammalian enzyme differs
from  the  bacterial  or  yeast proteins by having an  EF-hand calcium-binding
region (See <PDOC00018>) in its C-terminal extremity.

We developed  two  signature patterns. One based on the first half of the FAD-
binding  domain and one which corresponds to a conserved region in the central
part of these enzymes.

-Consensus pattern: [IV]-G-G-G-x(2)-G-[STACV]-G-x-[AT]-x-[DQ]-x(3)-[RAS]-G
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Consensus pattern: G-G-K-x(2)-[GSTE]-Y-R-x(2)-A
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Expert(s) to contact by email:
           Brown L.J.; ljbrown2@facstaff.wisc.edu

-Last update: December 2004 / Pattern and text revised.

[ 1] Austin D., Larson T.J.
     "Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol
     3-phosphate dehydrogenase of Escherichia coli K-12."
     J. Bacteriol. 173:101-107(1991).
     PubMed=1987111
[ 2] Roennow B., Kielland-Brandt M.C.
     Yeast 9:1121-1130(1993).
[ 3] Brown L.J., McDonald M.J., Lehn D.A., Moran S.M.
     "Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase
     cDNA. Evidence for EF-hand calcium-binding domains."
     J. Biol. Chem. 269:14363-14366(1994).
     PubMed=8182039
{END}
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