{PDOC00813}
{PS01062; HMG_COA_LYASE}
{BEGIN}
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* Hydroxymethylglutaryl-coenzyme A lyase active site *
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3-hydroxy-3-methylglutaryl-coenzyme A lyase (HMG-CoA lyase or HL) (EC 4.1.3.4)
catalyzes the transformation of HMG-CoA into acetyl-CoA and acetoacetate. In
vertebrates it is a mitochondrial enyme which is involved in ketogenesis and
in leucine catabolism [1]. In some bacteria, such as Pseudomonas mevalonii,
it is involved in mevalonate catabolism (gene mvaB). A cysteine has been shown
[2], in mvaB, to be required for the activity of the enzyme. The region around
this residue is perfectly conserved and is used as a signature pattern.
-Consensus pattern: S-V-A-G-L-G-G-C-P-Y
[C is the active site residue]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: November 1995 / First entry.
[ 1] Mitchell G.A., Robert M.-F., Hruz P.W., Wang S., Fontaine G.,
Behnke C.E., Mende-Mueller L.M., Schappert K., Lee C., Gibson K.M.
"3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL). Cloning of human
and chicken liver HL cDNAs and characterization of a mutation causing
human HL deficiency."
J. Biol. Chem. 268:4376-4381(1993).
PubMed=8440722
[ 2] Hruz P.W., Narasimhan C., Miziorko H.M.
"3-Hydroxy-3-methylglutaryl coenzyme A lyase: affinity labeling of the
Pseudomonas mevalonii enzyme and assignment of cysteine-237 to the
active site."
Biochemistry 31:6842-6847(1992).
PubMed=1637819
{END}