PMID:
     11503206
Authors:
     Lanne B, Potthast F, Hoglund A, Brockenhuus von Lowenhielm H, Nystrom AC, Nilsson F,
     Dahllof B.
Title:
     Thiourea enhances mapping of the proteome from murine white adipose tissue.
Journal:
     Proteomics. 2001 Jul;1(7):819-28. doi: 
Abstract:
     Adipose tissue imposes problems in two-dimensional (2-D) analysis due to its 
     extremely high content of fat. To improve protein separation detergents and 
     chaotropes were varied in the IEF step. The most important factor for obtaining 
     distinct spots in the 2-D gel was whether thiourea was included or not. Many high 
     molecular weight spots became resolved by using thiourea, while no spots 
     disappeared or showed inferior characteristics, thus approximately twice as many 
     spots were possible to quantify. Hydrophobic indices were compared for a set of 
     proteins that gave rise to sharper spots with proteins that were not improved on 
     the use of thiourea. The comparison did not give any statistically significant 
     difference between the two groups of proteins. One of the effects obtained by 
     inclusion of thiourea was that the dominating protein, serum albumin, appeared as 
     more condensed spots allowing other minor proteins to be detected. This work 
     resulted in a protocol which greatly enhances the resolution of proteins in 
     adipose tissue. A 2-D map of mouse white adipose tissue from epididymal fat pads 
     was constructed in which 140 spots were identified by mass spectrometry. This 
     work lays the ground for our further studies on white adipose tissue in metabolic 
     diseases such as obesity and dyslipidemia.

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