Database: PubMedEntry: 11680886
Original site: 11680886
Tonella L, Hoogland C, Binz PA, Appel RD, Hochstrasser DF, Sanchez JC.
New perspectives in the Escherichia coli proteome investigation.
Proteomics. 2001 Mar;1(3):409-23. doi:
Escherichia coli is a model organism for biochemical and biological studies as it
is one of the best characterised prokaryote. Two-dimensional polyacrylamide gel
electrophoresis, computer image analysis and different protein identification
techniques gave rise, in 1995, to the Escherichia coli SWISS-2D PAGE database
(http://www.expasy.ch/ch2d/). In the E. coli 3.5-10 SWISS-2D PAGE map, 40% of the
E. coli proteome was displayed. The present study demonstrated that the use of
narrow range pH gradients is able to potentially display up to a few copies of
protein per E. coli cell. Moreover, the six new E. coli SWISS-2D PAGE maps (pH
4-5, 4.5-5.5, 5-6, 5.5-6.7, 6-9 and 6-11) presented here displayed altogether
more than 70% of the entire E. coli proteome.
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