Database: PubMedEntry: 12429849
Original site: 12429849
Scherl A, Coute Y, Deon C, Calle A, Kindbeiter K, Sanchez JC,
Greco A, Hochstrasser D, Diaz JJ.
Functional proteomic analysis of human nucleolus.
Mol Biol Cell. 2002 Nov;13(11):4100-9. doi: 10.1091/mbc.e02-05-0271.
The notion of a "plurifunctional" nucleolus is now well established. However,
molecular mechanisms underlying the biological processes occurring within this
nuclear domain remain only partially understood. As a first step in elucidating
these mechanisms we have carried out a proteomic analysis to draw up a list of
proteins present within nucleoli of HeLa cells. This analysis allowed the
identification of 213 different nucleolar proteins. This catalog complements that
of the 271 proteins obtained recently by others, giving a total of approximately
350 different nucleolar proteins. Functional classification of these proteins
allowed outlining several biological processes taking place within nucleoli.
Bioinformatic analyses permitted the assignment of hypothetical functions for 43
proteins for which no functional information is available. Notably, a role in
ribosome biogenesis was proposed for 31 proteins. More generally, this functional
classification reinforces the plurifunctional nature of nucleoli and provides
convincing evidence that nucleoli may play a central role in the control of gene
expression. Finally, this analysis supports the recent demonstration of a
coupling of transcription and translation in higher eukaryotes.
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