Wang L, Zhu YF, Guo XJ, Huo R, Ma X, Lin M,
Zhou ZM, Sha JH.
A two-dimensional electrophoresis reference map of human ovary.
J Mol Med (Berl). 2005 Oct;83(10):812-21. doi: 10.1007/s00109-005-0676-y. Epub
The ovary plays a central role in oogenesis and gonadal hormone secretion.
Proteomic analysis is a valuable approach for gaining an increased understanding
of the molecular nature of the ovary. In this work, two-dimensional
electrophoresis for protein separation followed by matrix-assisted laser
desorption/ionization mass spectrometry and database searches, identified 231
protein spots corresponding to 138 individual proteins that were found in gels
representing both the follicular and luteal phases. The data were used to
construct a database online (http://reprod.njmu.edu.cn/2d). The identified
proteins were functionally classified into seven groups: (1) cell
signaling/communication, (2) cell division, (3) gene/protein expression, (4)
metabolism, (5) cell structure and motility, (6) cell/organism defense, and (7)
unclassified. Among the proteins identified, 47% had not been previously reported
in the human ovary. In addition, a number of disease-related proteins were
identified in this protein map, including some cancer- and polycystic ovarian
syndrome-related proteins. Two proteins with phosphorylation were verified by
Western blot analysis. Comparison of protein abundance between follicular and
luteal stages produced seven protein spots that had been identified in our
database. This study provides a preliminary reference map of normal human ovary
that will form a basis for comparative studies on normal and pathological
conditions of the human ovary and may serve as a potential tool for clinical
diagnosis, therapeutics, and prognosis.
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