Huang XY, Guo XJ, Shen J, Wang YF, Chen L, Xie J,
Wang NL, Wang FQ, Zhao C, Huo R, Lin M, Wang X,
Zhou ZM, Sha JH.
Construction of a proteome profile and functional analysis of the proteins
involved in the initiation of mouse spermatogenesis.
J Proteome Res. 2008 Aug;7(8):3435-46. doi: 10.1021/pr800179h. Epub 2008 Jun 27.
Spermatogenesis is a complex process of terminal differentiation wherein mature
sperm are produced. In the first wave of mouse spermatogenesis, different
spermatogenic cells appear at specific time points, and their appearance is
expected to be accompanied by changes in specific protein expression patterns. In
this study, we used 2D-PAGE and MALDI-TOF/TOF technology to construct a
comparative proteome profile for mouse testis at specific time points (days 0, 7,
14, 21, 28, and 60 postpartum). We identified 362 differential protein spots
corresponding to 257 different proteins. Further cluster analysis revealed 6
expression patterns, and bioinformatics analysis revealed that each pattern was
related to many specific cell processes. Among them, 28 novel proteins with
unknown functions neither in somatic cells nor germ cells were identified, 8 of
which were found to be uniquely or highly expressed in mouse testes via
comparison with the GNF SymAtlas database. Further, we randomly selected 7
protein spots and the above 8 novel proteins to verify the expression pattern via
Western blotting and RT-PCR, and 6 proteins with little information in testis
were further investigated to explore their cellular localization during
spermatogenesis by performing immunohistochemistry for the mouse testis tissue.
Taken together, the above results reveal an important proteome profile that is
functional during the first wave of mouse spermatogenesis, and they provide a
strong basis for further research.
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