Database: PubMedEntry: 6988414
Original site: 6988414
Bloch PL, Phillips TA, Neidhardt FC.
Protein identifications of O'Farrell two-dimensional gels: locations of 81
Escherichia coli proteins.
J Bacteriol. 1980 Mar;141(3):1409-20. doi: 10.1128/jb.141.3.1409-1420.1980.
The two-dimensional gel electrophoresis method of P. H. O'Farrell readily
resolves approximately 1,000 proteins from whole-cell homogenates. We have found
that the location of most individual proteins is sufficiently reproducible and
precise to permit different laboratories to exchange information about them. We
present the location of 81 Escherichia coli structural proteins, binding
proteins, enzymes, and factors, identified with the aid of purified proteins
supplied to us by many investigators.
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