GenomeNet

Database: PubMed
Entry: 8740179
LinkDB: 8740179
Original site: 8740179 
PMID:
     8740179
Authors:
     Pasquali C, Frutiger S, Wilkins MR, Hughes GJ, Appel RD, Bairoch A,
     Schaller D, Sanchez JC, Hochstrasser DF.
Title:
     Two-dimensional gel electrophoresis of Escherichia coli homogenates: the
     Escherichia coli SWISS-2DPAGE database.
Journal:
     Electrophoresis. 1996 Mar;17(3):547-55. doi: 10.1002/elps.1150170325.
Abstract:
     Numerous Escherichia coli proteins have already been characterized by
     two-dimensional gel electrophoresis (2-D PAGE), using carrier ampholytes in the
     first dimension (VanBogelen, R. A., Sankar, P., Clark, R. L., Bogan, J. A. and
     Neidhardt, F. C., Electrophoresis 1992, 13, 1014-1054). We present here a
     reference protein map of E. coli obtained with immobilized pH gradients (IPG) and
     available in a SWISS-2DPAGE format. Out of the protein spots identified in the E.
     coli gene protein database by Neidhardt's group, 153 have been identified in the 
     E. coli gene protein database by Neihardt's group, 153 have been identified on
     the E. coli SWISS-2DPAGE database map by gel comparison and most of them were
     confirmed either by the analysis of amino acid composition (AAC) and/or
     N-terminal microsequencing. Additionally, five as yet unsequenced proteins were
     found. The E. coli SWISS-2DPAGE database is part of the ExPASy molecular biology 
     server accessible through the Word Wide Web network.

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