Database: PubMed
Entry: 8740185
LinkDB: 8740185
Original site: 8740185 
     Lutomski D, Caron M, Cornillot JD, Bourin P, Dupuy C, Pontet M,
     Bladier D, Joubert-Caron R.
     Identification of different galectins by immunoblotting after two-dimensional 
     polyacrylamide get electrophoresis with immobilized pH gradients.
     Electrophoresis. 1996 Mar;17(3):600-6. doi: 10.1002/elps.1150170331.
     Vertebrate soluble beta-galactoside-binding lectins form a growing protein family 
     that recently have been named galectins. Seven different galectins have been 
     sequenced and characterized in mammals, and there is compelling evidence for the 
     existence of other members of this lectin family. Three among six galectins are 
     homodimers with (i) an identical subunit of a relative molecular mass of about 
     14500, and (ii) amino acid sequence homologies giving rise to possible 
     immunochemical cross-reactivities. They are indistinguishable from each other by 
     conventional sodium dodecyl sulfate-polyacrylamide gel electrophoresis 
     (SDS-PAGE), even when followed by immunoblotting. However, their different 
     isoelectric points allow their identification using isoelectric focusing and 
     two-dimensional (2-D) polyacrylamide gel electrophoresis. A strategy was 
     developed to identify these galectins in crude extracts from cells and tissues, 
     based on the two-dimensional electrophoresis with immobilized pH gradient 
     (IPG-Dalt) analysis of the specific spots of purified galectins and of the spots 
     of crude extracts, after silver staining. In addition, 2-D immunoblotting using 
     anti-galectin 1 (Gal-1) and anti carbohydrate-binding protein 15 (CPB15) 
     antibodies were performed on brain and leukemia cells (HL60) allowing an 
     identification of related polypeptides. Our results indicate that the use of 
     IPG-Dalt provides a suitable reproducibility and allows the detection of 
     galectins or other galactoside-binding proteins even at basic pIs.

This page is constructed based on the NCBI service.
  All links  
No link information was found.   
DBGET integrated database retrieval system