LOCUS NC_031671 2773601 bp DNA linear CON 25-OCT-2016
DEFINITION Sugiyamaella lignohabitans strain CBS 10342 chromosome C, complete
sequence.
ACCESSION NC_031671
VERSION NC_031671.1
DBLINK BioProject: PRJNA342695
BioSample: SAMN04417247
Assembly: GCF_001640025.1
KEYWORDS RefSeq.
SOURCE Sugiyamaella lignohabitans
ORGANISM Sugiyamaella lignohabitans
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Trichomonascaceae;
Sugiyamaella.
REFERENCE 1 (bases 1 to 2773601)
AUTHORS Bellasio,M., Peymann,A., Valli,M., Sipitzky,M., Graf,A., Sauer,M.,
Marx,H. and Mattanovich,D.
TITLE Complete genome sequence and transcriptome regulation of the
pentose utilising yeast Sugiyamaella lignohabitans
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2773601)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (25-OCT-2016) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 2773601)
AUTHORS Peymann,A. and Graf,A.
TITLE Direct Submission
JOURNAL Submitted (18-FEB-2016) Department of Biotechnology, University of
Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna
1190, Austria
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to CP014500.
##Genome-Annotation-Data-START##
Annotation Provider :: INSDC submitter
Annotation Status :: Full annotation
Annotation Pipeline :: Eukaryotic Annotation Propagation Pipeline
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..2773601
/organism="Sugiyamaella lignohabitans"
/mol_type="genomic DNA"
/strain="CBS 10342"
/type_material="culture from holotype of Sugiyamaella
lignohabitans"
/db_xref="taxon:796027"
/chromosome="C"
gene <2548..>3243
/gene="RIP1"
/locus_tag="AWJ20_3707"
/db_xref="GeneID:30035758"
mRNA <2548..>3243
/gene="RIP1"
/locus_tag="AWJ20_3707"
/product="ubiquinol--cytochrome-c reductase catalytic
subunit RIP1"
/transcript_id="XM_018880729.1"
/db_xref="GeneID:30035758"
CDS 2548..3243
/gene="RIP1"
/locus_tag="AWJ20_3707"
/inference="similar to AA sequence:KEGG_Orthology:K00411"
/note="Ubiquinol-cytochrome-c reductase; a Rieske
iron-sulfur protein of the mitochondrial cytochrome bc1
complex; transfers electrons from ubiquinol to cytochrome
c1 during respiration; during import, Rip1p is first
imported into the mitochondrial matrix where it is
processed, acquires its Fe-S cluster, and is folded, then
is translocated into the inner membrane by the action of a
homo-oligomer of Bcs1p, and finally is delivered by Bcs1p
to Complex III for assembly; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005743 - mitochondrial inner membrane
[Evidence IEA,IEA]; GO_component: GO:0005750 -
mitochondrial respiratory chain complex III [Evidence IDA]
[PMID 10873857]; GO_component: GO:0005750 - mitochondrial
respiratory chain complex III [Evidence IDA] [PMID
3036836]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005739 -
mitochondrion [Evidence IPI] [PMID 16962558];
GO_component: GO:0070469 - respiratory chain [Evidence
IEA]; GO_function: GO:0051537 - 2 iron, 2 sulfur cluster
binding [Evidence IEA,IEA]; GO_function: GO:0051536 -
iron-sulfur cluster binding [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_function: GO:0016679 -
oxidoreductase activity, acting on diphenols and related
substances as donors [Evidence IEA]; GO_function:
GO:0008121 - ubiquinol-cytochrome-c reductase activity
[Evidence IEA,IEA]; GO_process: GO:0009060 - aerobic
respiration [Evidence IMP] [PMID 3036836]; GO_process:
GO:1902600 - hydrogen ion transmembrane transport
[Evidence IEA,IEA]; GO_process: GO:0006122 - mitochondrial
electron transport, ubiquinol to cytochrome c [Evidence
IDA] [PMID 3036836]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase catalytic
subunit RIP1"
/protein_id="XP_018733390.1"
/db_xref="GeneID:30035758"
/translation="MNTLLRATSSAARAALRTSQQSSAFAMASGSSRFVSNNNKPVGG
ASSYKVPDFTPYMKENRNEDSNRAFSYFMVGTMGVISAAAARATVQDFLTTMSASADV
LALAKVEVALGNIPEGKNIIVKWRGKPVFIRHRTPAEIHEANEVNVASLRDPQTDDQR
VKMPEWLVMVGICTHLGCVPIGESGDYGGWFCPCHGSHYDISGRIRKGPAPLNLEIPE
YEFIDNNEKVVIG"
gene <4851..>7451
/gene="STE11"
/locus_tag="AWJ20_3708"
/db_xref="GeneID:30035759"
mRNA <4851..>7451
/gene="STE11"
/locus_tag="AWJ20_3708"
/product="mitogen-activated protein kinase kinase kinase
STE11"
/transcript_id="XM_018880730.1"
/db_xref="GeneID:30035759"
CDS 4851..7451
/gene="STE11"
/locus_tag="AWJ20_3708"
/inference="similar to AA sequence:KEGG_Orthology:K11228"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
STE11"
/protein_id="XP_018733391.1"
/db_xref="GeneID:30035759"
/translation="MLTNRTWSSTDIKQWLESLHCGEYFQSFEDNHITGDVLLECDQT
ALKYMGIRKVGDRIKINQSLKELRSDYVKSLAEARGHSLFRYGPQSLEDLNRNSVSPF
AALSSSQPSKPEHSTPAEGYFSPKHSSSAGPGSALGILSGLSLGVPPNSSSSSRFPFR
RTAPNSAKSQYSTATTTTTVLSKTDSNGVQEIFSMDVVKQNNVKFIYPQGESKTVNIA
GCTTADEIKRKALQKIMADNVVNPVEWIVFVLLEDKFRHLGDVELMSIAQSSDSSERK
RLMLCAIGTSPSTKQLEKSKQIQVNSHLKLSSLSNIDSADSIAQATKPESKSISTNRL
SLAQSIAATSYDDDDLDDDDLEQDGASVTANARPRKVGRLSEHRPPSELISSNLAHFF
PSAPANDLEETIRNSRRFSKRMSRMSRISHRMSVASSTWGIPEDEEAPPPVPALPDHG
EQGTIMPGSLPSPYTSTTPTIPKPTTPTTAPVPTTEAPAAPEAKRPSLRSSIRKSVMP
RRYSSYESGNTNMLTALSNRISTMSLSKGGFSKSHNGGSEDQIHDGPTSDNEPETDAN
QEQHAPEVEDEDDEEDDNTFLDTVRKEESCPSKWIKGRLIGSGSFGTVYLGMNSFTGE
LMAVKQVEIPTEDTENGQRKKSMVDALQREMNLLRELQHKNIVQYLGSNSEGQFLNIF
LEYVPGGSVAMLLSNYGIFEESLIRNFVRQILEGLKYLHGQNIIHRDIKGANVLVDNK
GCIKISDFGISKKIETKLLTNNRVSLQGSVFWMAPEVVKQLPYTLKADIWSLGCLIVE
MFSGTHPFPEFTQMQVIFRLGNSGTPAIPDDCSDDAKDFLAKTFAIDHQKRPTAAELL
SHKFMDPIPQ"
gene complement(<7585..>9435)
/locus_tag="AWJ20_3709"
/db_xref="GeneID:30035760"
mRNA complement(<7585..>9435)
/locus_tag="AWJ20_3709"
/product="hypothetical protein"
/transcript_id="XM_018880731.1"
/db_xref="GeneID:30035760"
CDS complement(7585..9435)
/locus_tag="AWJ20_3709"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733392.1"
/db_xref="GeneID:30035760"
/translation="MNTDKTGGSTVYGPDHPDSQHYEDSDSAMATAIQQSIDSSNNNF
KAVEGNYYQKYGEQESGELQKFGPATRESYDSKQWGIVPYSEASSSTSTVAAGLQQAQ
GSTTPIIYEFRNGIPIVLPADDAIYIAPLMMIIHSIPLGRKALLNGGVDLVPDYGFDP
LWWLGEKLIDLQDGPIHNRERLMIEIQRYTAFLDGESRRPCALIAGVISEAGPRASKL
ILKSNSQDEKPIGRFLQDLVKFWGPDSEFANVFQSTAMQERPSSFEEEDGEVIQKFTN
LVTDVTVALQQSIYDFIDDLMSADGGAPTTFIKDISDIVNITIKRDDGNSGTGIEIPA
IWYPDRYSEEYFECVNLLHRRRTEFRKQLTDLNARRLEISSTKGKDISKLLNISYDYI
KEISEKASDEDKENFSLVEKELADVKNLYKHRKESIVEQVTQTQQIMLREANLFKGLL
SDEDKKYLAEIYPAAQSLPAMRPYRLRGLIISPTEFCFLRSQPADDDVDLVDLGNDGD
SEKSFRDPTWTKIVWGDLSENGSSELYVGQITQQDAINWAKQGSSGYKWQEVVAFYAN
DYATNVEAHKVALSEGLRKFIQADKQCLEDQVANVKEDEEIKVEHEEKSR"
gene complement(<10076..>13597)
/locus_tag="AWJ20_3710"
/db_xref="GeneID:30035762"
mRNA complement(<10076..>13597)
/locus_tag="AWJ20_3710"
/product="hypothetical protein"
/transcript_id="XM_018880733.1"
/db_xref="GeneID:30035762"
CDS complement(10076..13597)
/locus_tag="AWJ20_3710"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733393.1"
/db_xref="GeneID:30035762"
/translation="MGEEHGFKNGIDSQTDQPAYSPVEEGAYARPDGSRPGSRNRTED
STDGDVDSVPDSPSQALADFHDEWSISDPLLIKQAVIFPPSFFNPLRARNHDTEWDKK
IAQLRSLLTNDSSLLTSNNRNDSALDHVQLPYAHFIPSPQQSDISQYAVNSQVAQSLL
LSPKIKENRFQYSRGLLISYLSRDKRSSTWSDAITTVCCESPSLGTLQPTSKTHDNPI
NVIPNFPRAFLPPLFIYSAFAIYKDVVFTDTRCVRSRTGLVIAQVVQIMLSVFSAATI
TSVFFPQFCFSALYSFSALPAIIIMGSIENTLTLINSVSGTRPEISPTSRVAIAAQQA
FPKTVKAKLITAFLLSLVIMANLDKVINSIFLFSTLSVLIDLALHVTYFTAVLSVDIR
RLELAEVLLNPLDDEEPIVKHDSGTSSIPFFRQYQQLKGFRLRTFLYLNYLNPRQRYP
IVMASIVVIHMWLSLAGPLKDTATLQISSHFLDRTILFMNDLLNVHNYTVNIGSDVVP
LVIYNPIVLSFDDNSNCFHFSPFLSTTSSIIGGYSLFILFEFVLSLAIILLAAALILK
LLLPKRDCAEEDRAGLHSDSENKFSSKDLAGYHTLDVIQIVVNKSWIATVSLDHGICV
WNVCDGKRSGSKQDCKFRTLPPRLWPVSRVLVGLNSMLVIISAHNHVIEAWDFKNNKI
LYSIKDDELFPANSLLLLSFIVADQVVIITRSGAFVTISRTGTKETICPDLTFGRDVE
LIAATLLVTPKINDKVVCLFSDNSIVVVTHVAGTQWSFRSLDLLESLTAVSERRRGSK
DTVFYRPYPAYPPIEIAEASSPSFLVASNMNRASIPCTRPMEMKEKAHSVVSVPAIHM
VLVVSELKASLFDVHTGVIVRHFQLGLYKPDTIRVFHAQPTHCRFCGCASIESISVAY
YDQEDKGLVICHTVQIENRAKNNICLRVERDPRETRCLGFEAATEQVHWIDRVEGWDT
TDINMMMGVRRKENPQSSGSPSPDSGRRSPATGTERRDLSMSATTSVLRRISNKLSQG
ASSTSSASYSTPQPPTHGQKKPPLNITWEGWAMTGKGKVSYYNIPDVPGKPGSYNIQN
LSRDDYIPQASSRLLIRTVGPAVKYGKKSIAVAFGNVIKIMYFGNEEFHDEIIYGNRL
VGIPSYESAPPSNTPSASRRWKREIAY"
gene <16767..>17999
/gene="YDJ1"
/locus_tag="AWJ20_3711"
/db_xref="GeneID:30035763"
mRNA <16767..>17999
/gene="YDJ1"
/locus_tag="AWJ20_3711"
/product="type I HSP40 co-chaperone YDJ1"
/transcript_id="XM_018880734.1"
/db_xref="GeneID:30035763"
CDS 16767..17999
/gene="YDJ1"
/locus_tag="AWJ20_3711"
/inference="similar to AA sequence:KEGG_Orthology:K09503"
/note="Type I HSP40 co-chaperone; involved in regulation
of HSP90 and HSP70 functions; critical for determining
cell size at Start as a function of growth rate; involved
in protein translocation across membranes; member of the
DnaJ family; GO_component: GO:0072380 - TRC complex
[Evidence IDA] [PMID 20850366]; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005829 -
cytosol [Evidence IDA] [PMID 1869583]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 8144572];
GO_component: GO:0048471 - perinuclear region of cytoplasm
[Evidence IEA]; GO_component: GO:0048471 - perinuclear
region of cytoplasm [Evidence IDA] [PMID 1869583];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0001671 - ATPase activator activity
[Evidence IDA] [PMID 1400408]; GO_function: GO:0001671 -
ATPase activator activity [Evidence IDA] [PMID 15342786];
GO_function: GO:0031072 - heat shock protein binding
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0051082 - unfolded
protein binding [Evidence IEA]; GO_function: GO:0051082 -
unfolded protein binding [Evidence IDA] [PMID 9774392];
GO_process: GO:0006458 - 'de novo' protein folding
[Evidence IMP] [PMID 10567418]; GO_process: GO:0030433 -
ER-associated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 15252059]; GO_process:
GO:0030433 - ER-associated ubiquitin-dependent protein
catabolic process [Evidence IGI,IMP] [PMID 15342786];
GO_process: GO:0006457 - protein folding [Evidence IEA];
GO_process: GO:0042026 - protein refolding [Evidence IDA]
[PMID 9674429]; GO_process: GO:0045047 - protein targeting
to ER [Evidence IMP] [PMID 1473150]; GO_process:
GO:0006626 - protein targeting to mitochondrion [Evidence
IMP] [PMID 1729605]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0009408 -
response to heat [Evidence IEA]; GO_process: GO:0006950 -
response to stress [Evidence IEA]; GO_process: GO:0006810
- transport [Evidence IEA]"
/codon_start=1
/product="type I HSP40 co-chaperone YDJ1"
/protein_id="XP_018733394.1"
/db_xref="GeneID:30035763"
/translation="MVKDTKLYDILGVSPDATDSQIKKAYRVNALKYHPDKNQHSPEA
ADKFKEVSSAYEVLSDEQKRQVYDTYGMEGLSGQGGPDMGNAEDLFSHFFGGMGGMGG
MGGMFGGGAPSGPRKSKDIMHAIRATLEDLYKGKVSKLALKKTVICKTCDGKGGKEGA
VKTCPGCKGQGMKFVTRQMGNVIQRFQTVCPDCNGEGQIIDPKDRCKTCLGKKTTEEK
KILQVHIDKGMANGQKIVFQGEGDQGPGIIPGDVIFVVNEQPHEKFERRGDDLLYKVK
IDLLTALAGGSFGIKHLDGEWLKVEIIPGEVIAPGSFKLIEGKGMPSYRHHNFGNMIV
EFEVEFPKDNFATPEQLAALETVLPPRAKLEIPADVHAEEVILADLDPSKYQSGGAME
EDDDDMHAGGERVQCASQ"
gene complement(<18485..>19930)
/gene="MSG5"
/locus_tag="AWJ20_3712"
/db_xref="GeneID:30035764"
mRNA complement(<18485..>19930)
/gene="MSG5"
/locus_tag="AWJ20_3712"
/product="tyrosine/serine/threonine protein phosphatase
MSG5"
/transcript_id="XM_018880735.1"
/db_xref="GeneID:30035764"
CDS complement(18485..19930)
/gene="MSG5"
/locus_tag="AWJ20_3712"
/inference="similar to AA sequence:KEGG_Orthology:K11240"
/codon_start=1
/product="tyrosine/serine/threonine protein phosphatase
MSG5"
/protein_id="XP_018733395.1"
/db_xref="GeneID:30035764"
/translation="MKSPHISPPISPNSFSNPKSHRSSTRNRNASSLSISIPISPQKI
PPLESATYRRSSGASSFPEYSSGCTGTSSTSTDTCSGAGSGAGSNITPNVVYLDSDSN
SIYRASSSSSSTTTTTTLHYQQFPQFSSGSSLPNIGSTEPQNSNSYYYYQQPLKTSTN
TNFEVMPAENPYPNGPVLVREPNIYLYSEPSIQTASQFDVIVNVAREVPNPFEQTSRL
SKEISSPMAPPPPAPFVKGARSSSSSSSSFTSNLSCSPLSSTSTHTEDTCFSPTGYPE
NSPPSSVNSTPNLNEELSSENDCEEETDEMTLRDLSSPITQSSTTAAFGLASSESLNG
RTTPEYIYVPWDHTSKLTPDLARLTDLISSRSAEGKKILVHCQCGVSRSASLVVAYVM
RQDKCDLNTAYDHVKKRSPNISPNMTLMFQLIDWARMLKRNPENPGVSTSLGPSLSSP
PSSYDDDLIPSASGYSLASPPPSTPTTAVNH"
gene complement(<23143..>23877)
/gene="MIC26"
/locus_tag="AWJ20_3713"
/db_xref="GeneID:30035765"
mRNA complement(<23143..>23877)
/gene="MIC26"
/locus_tag="AWJ20_3713"
/product="Mic26p"
/transcript_id="XM_018880736.1"
/db_xref="GeneID:30035765"
CDS complement(23143..23877)
/gene="MIC26"
/locus_tag="AWJ20_3713"
/inference="similar to AA sequence:KEGG_Orthology:K17786"
/note="Component of the MICOS complex; MICOS (formerly
MINOS or MitOS) is a mitochondrial inner membrane complex
that extends into the intermembrane space and has a role
in the maintenance of crista junctions, inner membrane
architecture, and formation of contact sites to the outer
membrane; Mic26p is a non-essential component of the
complex; GO_component: GO:0061617 - MICOS complex
[Evidence IDA] [PMID 21944719]; GO_component: GO:0061617 -
MICOS complex [Evidence IDA] [PMID 21987634];
GO_component: GO:0061617 - MICOS complex [Evidence IDA]
[PMID 22009199]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0044284 - mitochondrial crista junction [Evidence IDA]
[PMID 22009199]; GO_component: GO:0005743 - mitochondrial
inner membrane [Evidence IEA,IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14562095];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0042407 -
cristae formation [Evidence IMP] [PMID 21987634];
GO_process: GO:0042407 - cristae formation [Evidence IMP]
[PMID 22009199]"
/codon_start=1
/product="Mic26p"
/protein_id="XP_018733396.1"
/db_xref="GeneID:30035765"
/translation="MKLLTGQKQLLTLLGAGIVSYSAIPVAMADSKKPIYDDEEKNEE
TVVVVQPEETQLINTEYVGNTPIKSSKFLEENIEVARKWVYEQSQTVQAKVDDSFQTY
LQAERSVTSTVSNLKDENEDILPGGIYILIGALSGSIFARRRGFVLRTVTPVIFGLGA
FSYFLPKTFNNTRALVWSFEQKSPAIADTHIQIQNQVESLAESAKSAAEQSQSSLESG
VHSVRQFVAESTGLQIGSSSSSNNKK"
gene complement(<24512..>26161)
/gene="FRE3"
/locus_tag="AWJ20_3714"
/db_xref="GeneID:30035766"
mRNA complement(<24512..>26161)
/gene="FRE3"
/locus_tag="AWJ20_3714"
/product="Fre3p"
/transcript_id="XM_018880737.1"
/db_xref="GeneID:30035766"
CDS complement(24512..26161)
/gene="FRE3"
/locus_tag="AWJ20_3714"
/note="Ferric reductase; reduces siderophore-bound iron
prior to uptake by transporters; expression induced by low
iron levels; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016021 - integral component
of membrane [Evidence ISS] [PMID 9726978]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 11120744]; GO_function: GO:0052851 - ferric-chelate
reductase (NADPH) activity [Evidence IEA]; GO_function:
GO:0000293 - ferric-chelate reductase activity [Evidence
IGI] [PMID 11120744]; GO_function: GO:0000293 -
ferric-chelate reductase activity [Evidence ISA] [PMID
9726978]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_process: GO:0006879 -
cellular iron ion homeostasis [Evidence IEP,IMP] [PMID
10341420]; GO_process: GO:0006879 - cellular iron ion
homeostasis [Evidence IEP,IMP] [PMID 9726978]; GO_process:
GO:0006811 - ion transport [Evidence IEA]; GO_process:
GO:0055072 - iron ion homeostasis [Evidence IEA];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]; GO_process: GO:0015891 - siderophore
transport [Evidence IMP] [PMID 9726978]"
/codon_start=1
/product="Fre3p"
/protein_id="XP_018733397.1"
/db_xref="GeneID:30035766"
/translation="MLAFWGIIILFKSIHNLFHRMGPYVLKYLDFPLSRRLRAEFFLP
PTGRYHHSTPKSLLGFTFNIPARIETIIISLFFIQNILLAFVGIETIENSVFYDSMSD
QYADSIGYRAGVICMAELVLLIVFAGRNNLLIYITGWEMNTFNVFHKWVARMTAMQAV
IHSIGYTIEYVQEDYYTEYFSGEPGTAWFIWGVVATILMCALITHSVHFFRSINYELF
LYGHITLTAVAIAGVWLHVIGMEGMNYTYAAIAIWAFDHVARIVRLLWSGVTSKASMK
LHGEHILEIKIDYSKRWKFYPGCHVYLHILRTNRFWQSHPFTVVTSPRPDDQGKLVIF
ARVKKGLTKHTANYLKDKPDQSAQVRVLVEGPYGGRHPLHHYETVVLIAGGVGITSIY
PYAAQLRENKDKAQRMVFIWVIRNEQPLSWFQDQLRHISEDPRIEVILFITGSNALVS
NEGSATSSTTNLDKSPLESEASSQTEKKSPLVSSSVFDIRAGTPDLRSRVAHDISEAA
GTVAFLVCGPNRMNDDVRMGIVDNIADSKHRVDYFEDSFSW"
gene <28670..>29821
/locus_tag="AWJ20_3715"
/db_xref="GeneID:30035767"
mRNA <28670..>29821
/locus_tag="AWJ20_3715"
/product="hypothetical protein"
/transcript_id="XM_018880738.1"
/db_xref="GeneID:30035767"
CDS 28670..29821
/locus_tag="AWJ20_3715"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733398.1"
/db_xref="GeneID:30035767"
/translation="MSQLFSSSDSCGPVNPLQNLGKHTGPTNSSQHERFVRENSAGPS
SFRSTPSPSLVNGTNTDFQAFASRGSPSQSGFNFHPGQGQAASQVPGQLPGQLPVQLA
RNGAPLDQRNIGFQAPQTTSSDWASEFSKLSVGSRPSQDLRMATSPLSMTAASSSYQQ
YQPQQPMLRSDLMYYQTSASVSQQPEHHQIHKLNEEQASFENAFAEVERSLNSESISQ
QDTTIQKETEQVESLQKVNNTELSGVAQDIINSINESSQSTNSSTASKMKSSNFMALM
DQLSKKNVVLEGTKFVDGVSGAEISADQLARQEDTANYKEPSLPEHLQPQNMSKAPQI
PLQDPFSFINQLNIDPSKSTPFELAQLMSPGVALSDWEEKYEEDPDWTQ"
gene complement(<31853..>32113)
/locus_tag="AWJ20_3716"
/db_xref="GeneID:30035768"
mRNA complement(<31853..>32113)
/locus_tag="AWJ20_3716"
/product="hypothetical protein"
/transcript_id="XM_018880739.1"
/db_xref="GeneID:30035768"
CDS complement(31853..32113)
/locus_tag="AWJ20_3716"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733399.1"
/db_xref="GeneID:30035768"
/translation="MTELARDMILKSQQIEVLIDSLPGIGISEQEQMARVRSLEEELK
QVNNEKADVLTEREELLKKCDELILEVAREKVNLEKSQSMHS"
gene complement(<40106..>41998)
/gene="POP2"
/locus_tag="AWJ20_3717"
/db_xref="GeneID:30035769"
mRNA complement(<40106..>41998)
/gene="POP2"
/locus_tag="AWJ20_3717"
/product="CCR4-NOT core DEDD family RNase subunit POP2"
/transcript_id="XM_018880740.1"
/db_xref="GeneID:30035769"
CDS complement(40106..41998)
/gene="POP2"
/locus_tag="AWJ20_3717"
/inference="similar to AA sequence:KEGG_Orthology:K12581"
/codon_start=1
/product="CCR4-NOT core DEDD family RNase subunit POP2"
/protein_id="XP_018733400.1"
/db_xref="GeneID:30035769"
/translation="MMRASGNYNNRSNGPNAGLMSLQMLQGQGGQIQGQPQNQLQQQQ
QHQQQGVMPLQQQQYINAGAGYKQMLPDQRDDHGDDQQQMLQAQAQAQAQAQAQAQAQ
AHAHAQQAHVHQAQAHAHAHAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQ
AQAQAQAQAQAQAQAQAQGQPNLMFQRMQARNQNINQMAAASLQAFGGVPNVGAGADI
GVGAGGLGNGLMSSSTGGGPLSNYNNTTDINAFNGSLPNLGLAGGMMNIANTGGAGGA
LGGPTGNLAAAGGLSAAAAGGSMKSSTIVNVWKWNLEDQMAAIRDLVERYKYISVDCK
FPGIVARPIGTFKSTSEYHYQTLRSNVDILQVIQIGLTFADEYGNHPVGVASTFQFNF
KFNVNEDMCSADGVELLRQSGIDFAKHETDGIDPFAFGELILTSGLTLDDTVSWITFH
SGYDLGYILSIMLNNEVPVEERGFLSTLRTYFPNVWDVKCITKTFKLSNKSQLSEVAD
DFQVRRLPMPQSSVNGGLSSLGGLSQAGNDAFVTSGCFFEIKRLIGDANINKVRNYLF
GLGDGFEDDPSQQSNNGTNNAAAAASAAILNAISGGATASASASHGVTNAANIFQFGK
MGGGAL"
gene <46241..>49117
/gene="SSK2"
/locus_tag="AWJ20_3718"
/db_xref="GeneID:30035770"
mRNA <46241..>49117
/gene="SSK2"
/locus_tag="AWJ20_3718"
/product="mitogen-activated protein kinase kinase kinase
SSK2"
/transcript_id="XM_018880741.1"
/db_xref="GeneID:30035770"
CDS 46241..49117
/gene="SSK2"
/locus_tag="AWJ20_3718"
/inference="similar to AA sequence:KEGG_Orthology:K11230"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
SSK2"
/protein_id="XP_018733401.1"
/db_xref="GeneID:30035770"
/translation="MSKEKKFATPPLKIDLSSARAAVPINQGFRSTTPTIHSPFLLSP
GSQSLPSSTSSSSTSLHALAGGSFLGTSHGANHGYNNNVNGTSTGNNTGSNTGNGNGT
GNGNSSIASIGHSNGISGPSSPGGVSHGSVGPGNSNGSTGLSRHRSIGSIPTTHRSRR
DSGKSLGASGSMHTGLTAPARVSPRQSPRSSPRSSPRPRDGTGKEEDGGSYFTLSRPG
TASSPVNIPGTSSSSNSKMSLSSQTSYSGSSFDFDGQVQRVNSSLRKYDAATAGSPTG
TQPINASSPSMQSNNNALLSPTVSPRPHSPIRALSNSYAPARGPSHQNVSSVKTKGKA
PQSPIQDDSLTSNKSNNSPKQQQQQQQQQSNSFHHRSTAHASGATTAAAAVTAAANSD
ESPIFAKRKDAHLSYLAQEPVYIHKMTNAAGDDYVKGLIGDNTNYEYLSDDDDTDSIT
EVQPEVIGFGGVGTSFDDEYTELGSSYPFLETTYNSNGPSTSSATNNEPAGSNSHKDR
DKATASIESDGSTPERWRERLEWQAMLASVLTGEVFRAEKRRIKQPKKSGNGIDEEEM
WLALRAKVSGRTMNEQKRVLEYSRSTVTDALERVQNFKIENPANITAEDALKQLNPVL
QLLYRCESLYRTLAVLKGENAIYGNADFQTKVDCLLSWKSITEAINQELELLRKWTGN
YDIDPSRVPEKSSPSEEGSRLSMIQEEKQTLVESILKQDNLSNIFDRRISNRLGPLIE
KAREATIKYSDMFLELGLPLFNNSLLHVMVFPITLIREIIQMRLTYARRLVNPTLMIV
DQMILDFQSIISHALVVMDRNLQYTSPILDKGWIFPTQIDESFNSTLLDSVNFYLDLL
NKKFLDGGRSSKSLFRAYKETEQLEQHYTFLLGVGRLVEGGDLAVAELITSLESKILT
RLLTYWEHQMKGPQSWAPTEIERWFTTTMEIIRSFQRKLLRFHK"
gene <49255..>51228
/gene="SSK2"
/locus_tag="AWJ20_3719"
/db_xref="GeneID:30035771"
mRNA <49255..>51228
/gene="SSK2"
/locus_tag="AWJ20_3719"
/product="mitogen-activated protein kinase kinase kinase
SSK2"
/transcript_id="XM_018880742.1"
/db_xref="GeneID:30035771"
CDS 49255..51228
/gene="SSK2"
/locus_tag="AWJ20_3719"
/inference="similar to AA sequence:KEGG_Orthology:K11230"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
SSK2"
/protein_id="XP_018733402.1"
/db_xref="GeneID:30035771"
/translation="MSLLRLSGHFLVYTGTFEKYGIYAIADPYLKDRPETVRAILSGY
SRIPKKDVEHVSYVMIFCIEEPLVWEGEIVNLDMPYFDIDMKPGRMRLITEGGSQELK
RTRKEVSALTGGGDVFDIAVDTKSHITRVDHEMARVRKLFFKLSTSVITSCILFRRRC
KPYGCQETVHNMFVFAREFGQRGISVMDANRRGTVAIKLINLCIEWVTFICDDCVPTE
NKTFRWTVVALEFAMVMTRGVNIVAISGDQFAKLRVKVADCMTLLISHFDITGAKSRA
AQQGNSDVLRRGKALNISVDDDDELVAMLREDMVKKLELLEQQRQDTHSVGKVLDDSS
GDTEFLTYLASAFSNLQIRWQQGRYIGGGTFGSVYAAVNLDTGGVMAVKEIRLQDTQS
IRHILKSIKDEMTVLEILSHPNVVQYYGVEVHRDRVFIFMEYCQGGSLAGLLEYGRIE
DEQVIQVYTLQMLEGLAYLHQSGIVHRDIKPENILLDHNGVIKFVDFGAAKVIAKTGK
TRQASATNKSNATTGGNKTKLNSMTGTPMYMSPEVITGADSGRHGSIDIWSLGCCVLE
MATGRRPWANLDNEWAIMYHIAAGHLPQLPSADQLSIEGQQFLMKTLERDPKKRPSAV
DLLSDPWMVSIRNVVLGYSAGSDDSAVSSPSEG"
gene <53001..>55439
/gene="GEF1"
/locus_tag="AWJ20_3720"
/db_xref="GeneID:30035773"
mRNA <53001..>55439
/gene="GEF1"
/locus_tag="AWJ20_3720"
/product="Gef1p"
/transcript_id="XM_018880744.1"
/db_xref="GeneID:30035773"
CDS 53001..55439
/gene="GEF1"
/locus_tag="AWJ20_3720"
/inference="similar to AA sequence:KEGG_Orthology:K05012"
/note="Voltage-gated chloride channel; localized to the
golgi, the endosomal system, and plasma membrane; involved
in cation homeostasis; highly homologous to vertebrate
voltage-gated chloride channels; modulates TBSV model (+)
RNA virus replication by regulating copper metabolism;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0005794 - Golgi apparatus [Evidence IDA]
[PMID 15710404]; GO_component: GO:0005797 - Golgi medial
cisterna [Evidence IDA] [PMID 9614122]; GO_component:
GO:0000139 - Golgi membrane [Evidence IEA]; GO_component:
GO:0034707 - chloride channel complex [Evidence IEA];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IDA] [PMID 17662057]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0005768 - endosome [Evidence IEA];
GO_component: GO:0005768 - endosome [Evidence IDA] [PMID
15710404]; GO_component: GO:0010008 - endosome membrane
[Evidence IEA]; GO_component: GO:0000324 - fungal-type
vacuole [Evidence IDA] [PMID 17662057]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 17662057]; GO_component: GO:0005774 - vacuolar
membrane [Evidence IEA]; GO_component: GO:0005773 -
vacuole [Evidence IEA]; GO_function: GO:0030554 - adenyl
nucleotide binding [Evidence IEA]; GO_function: GO:0005254
- chloride channel activity [Evidence IEA]; GO_function:
GO:0005216 - ion channel activity [Evidence IEA];
GO_function: GO:0005247 - voltage-gated chloride channel
activity [Evidence IEA]; GO_function: GO:0005247 -
voltage-gated chloride channel activity [Evidence IDA]
[PMID 12074596]; GO_process: GO:0006878 - cellular copper
ion homeostasis [Evidence IMP] [PMID 9520490]; GO_process:
GO:0006878 - cellular copper ion homeostasis [Evidence
IMP] [PMID 9614122]; GO_process: GO:0006879 - cellular
iron ion homeostasis [Evidence IMP] [PMID 9520490];
GO_process: GO:1902476 - chloride transmembrane transport
[Evidence IEA]; GO_process: GO:0006821 - chloride
transport [Evidence IEA,IEA]; GO_process: GO:0034220 - ion
transmembrane transport [Evidence IEA]; GO_process:
GO:0006811 - ion transport [Evidence IEA]; GO_process:
GO:0044070 - regulation of anion transport [Evidence IEA];
GO_process: GO:0055085 - transmembrane transport [Evidence
IEA]; GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Gef1p"
/protein_id="XP_018733403.1"
/db_xref="GeneID:30035773"
/translation="MSSDSRVHLESLPSGTSVANDSIPDSLVTEIRRYEDFSTIDWVE
AASRENTLRIRTYKNAYGKSINSRRPSGPIDPVSENAEDSNAFGFDVLKGTGARYGLL
VRQMYSAAQSWLVLALVGACIGLIAGFLSIVTELLSDFKTGYCTAGLYLNENFCCSGY
DESDICPEWHRWSSFSGINYVIYIVFSTFFAFLAAFFVYSFAPYAAGSGISEIKCIIA
GFIMNGFLDAKTLFIKSVTLPLTIASGLSVGKEGPSVHYAVCVGNTVAGLFPKYRVYK
TKMREVLSSCAAAGVAVAFGSPMGGVLFSLEEMTSSFQLKSMWRSYFCALVATATLAS
VNPFRTGQLVLFSVKYDHDWHFFEIIFFILIGLFGGFGGLFIIKWNLRVQAFRKKYLG
NYAIQEATLLAFITAVICYFNEFLRIDMTESMQILFHECDHGFEDNVTCQPSHRMYLA
TTLIFGMVIRTILTIISYGSKVPAGIFVPSLAIGALFGRFVGTVVWWLHDTFPDATFF
SSCPPDAPCITPGTYALLGSAALLSGVMNITVTVVVIIFEVTGALRYILPTMIVVGVT
KAVGDYFGPGGGGIADRTIVANGYPFLEDDGDDEHNHYANDPIELAMTKDPIQIPSKK
FTISHIQSLLDGSSHQSYPVVDIDDEDASITSSIPTKSSTTATTINQTTNYTNNYLIG
YIWRSELEYIVAHNRDTPDRYVSFDSLESSEDSLNFSRLVNFSPVVVQNNTPLSKVIA
LFSKVGPRIVYVERNKSGTLCGLLTRKDILKYQHKSDYAMAPPDRSAAEEMDRKIWEL
LSQAISKVQELVRR"
gene complement(<56054..>57070)
/locus_tag="AWJ20_3721"
/db_xref="GeneID:30035774"
mRNA complement(<56054..>57070)
/locus_tag="AWJ20_3721"
/product="hypothetical protein"
/transcript_id="XM_018880745.1"
/db_xref="GeneID:30035774"
CDS complement(56054..57070)
/locus_tag="AWJ20_3721"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733404.1"
/db_xref="GeneID:30035774"
/translation="MADTEEYSSRRSASPDRYDRQRSLSPRRDSAQDGSNEAGLPDDR
ERNGDSGAANDSSNGNDAATSGSASGPVDDDEAAKNEGTNLFVSGLARQVTEAKLEEL
FSKYGTVVKCQIMVDPHTRDSRGFGFVDMADVEGADAAIAGINGQDLLDRVVSVEKAK
RKRPRTPTPGKYFGPPKTRGAGSFRRGGYGGPSRYGGGDYRGGYRPRYDDRDRYGGPP
PPGGYDRYDRYDRYDRYDRYDRYDDRFRGPPPPRGPPGPSRYDDRDGYRDREYSSRYD
EGPRDRYAPPHRRGDNYRDRERRGPPPSGGPGGDSRDYDRPSYRDEPRGAGYSPERED
YRRD"
gene <58198..>59196
/gene="WHI2"
/locus_tag="AWJ20_3722"
/db_xref="GeneID:30035775"
mRNA <58198..>59196
/gene="WHI2"
/locus_tag="AWJ20_3722"
/product="Whi2p"
/transcript_id="XM_018880746.1"
/db_xref="GeneID:30035775"
CDS 58198..59196
/gene="WHI2"
/locus_tag="AWJ20_3722"
/note="Protein required for full activation of the general
stress response; required with binding partner Psr1p,
possibly through Msn2p dephosphorylation; regulates growth
during the diauxic shift; negative regulator of G1 cyclin
expression; GO_component: GO:0005575 - cellular_component
[Evidence ND]; GO_function: GO:0019211 - phosphatase
activator activity [Evidence IMP] [PMID 12090248];
GO_process: GO:0007015 - actin filament organization
[Evidence IMP] [PMID 15020461]; GO_process: GO:0007049 -
cell cycle [Evidence IEA]; GO_process: GO:0006897 -
endocytosis [Evidence IMP] [PMID 15020461]; GO_process:
GO:0000422 - mitochondrion degradation [Evidence IMP]
[PMID 21429936]; GO_process: GO:0040008 - regulation of
growth [Evidence IMP] [PMID 2204680]; GO_process:
GO:0009408 - response to heat [Evidence IMP] [PMID
12090248]; GO_process: GO:0009651 - response to salt
stress [Evidence IMP] [PMID 12090248]"
/codon_start=1
/product="Whi2p"
/protein_id="XP_018733405.1"
/db_xref="GeneID:30035775"
/translation="MSIQPSSVITQVSATVPSAALHPFVSYDEQEKSENIQLNLRGVI
FNISREELLRLPESILIGITNGALYDQNTIGGASLADANAATANTLSPECFKYTLDVF
RSAAKGLPRTLLSIEGSEEQGFDDAILGVDEDTEGKSANSGVAMDADGNPKTETVSDI
SEILRRRPAIIVLREDLDYYCLPNGPNVDAEGMRQIKHSCGEAILNQRRIFGGLRKGE
SENSPEHHLIAMLCSSGFQIDDDWGFRAREPHKTVVSSLALIRLKMVDEQHHEQSPET
QQELSTPQKLLLFWRKPARKCWWDSIELNDVPGVDGPVRVHIRRVWTLELSVIGVR"
gene complement(<59408..>60247)
/gene="COQ3"
/locus_tag="AWJ20_3723"
/db_xref="GeneID:30035776"
mRNA complement(<59408..>60247)
/gene="COQ3"
/locus_tag="AWJ20_3723"
/product="hexaprenyldihydroxybenzoate methyltransferase"
/transcript_id="XM_018880747.1"
/db_xref="GeneID:30035776"
CDS complement(59408..60247)
/gene="COQ3"
/locus_tag="AWJ20_3723"
/note="O-methyltransferase; catalyzes two different
O-methylation steps in ubiquinone (Coenzyme Q)
biosynthesis; component of a mitochondrial
ubiquinone-synthesizing complex; phosphoprotein;
GO_component: GO:0019898 - extrinsic component of membrane
[Evidence IDA] [PMID 10419476]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0005743 - mitochondrial inner membrane
[Evidence IDA] [PMID 10419476]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 10760477];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0008425 -
2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
activity [Evidence IEA]; GO_function: GO:0061543 -
3-demethylubiquinone-6 3-O-methyltransferase activity
[Evidence IMP] [PMID 10419476]; GO_function: GO:0008689 -
3-demethylubiquinone-9 3-O-methyltransferase activity
[Evidence IEA]; GO_function: GO:0004395 -
hexaprenyldihydroxybenzoate methyltransferase activity
[Evidence IEA]; GO_function: GO:0004395 -
hexaprenyldihydroxybenzoate methyltransferase activity
[Evidence IMP] [PMID 1885593]; GO_function: GO:0008168 -
methyltransferase activity [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0032259 - methylation [Evidence IEA];
GO_process: GO:0006744 - ubiquinone biosynthetic process
[Evidence IEA,IEA,IEA]; GO_process: GO:0006744 -
ubiquinone biosynthetic process [Evidence IDA] [PMID
10419476]; GO_process: GO:0006744 - ubiquinone
biosynthetic process [Evidence IMP] [PMID 1885593]"
/codon_start=1
/product="hexaprenyldihydroxybenzoate methyltransferase"
/protein_id="XP_018733406.1"
/db_xref="GeneID:30035776"
/translation="MSHHEKVIAANLSSFKDKAAEYDNENTQLISRVVATKVLQFRFD
NAPDTEETEFASNDFDFNDLDIPNVADELWDPESTRVLDFACGTGLVSQSLAPYCKEI
VGVDISPDMVGIFNQKIENQGIPESEMRALVLDLLATDDDSLGQFDAVVTSLSYHHIH
DTDKCTKRLAGKLAPNGRLYVIDIEKGSGGSVHGRMTDEEALKEGVAHRGGLEADELV
QTFKNAGLDQVKVTRAFRVKLWVPEDKVKSFSGITGETKVVNGKTLHCSKVNLMLVTG
RRK"
gene complement(<61623..>61943)
/locus_tag="AWJ20_3724"
/db_xref="GeneID:30035777"
mRNA complement(<61623..>61943)
/locus_tag="AWJ20_3724"
/product="hypothetical protein"
/transcript_id="XM_018880748.1"
/db_xref="GeneID:30035777"
CDS complement(61623..61943)
/locus_tag="AWJ20_3724"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733407.1"
/db_xref="GeneID:30035777"
/translation="MSGSRGEANFVFRCKMCKRESSATIKLTGKTYGPDNSGKPVKVA
EVDNRGLEFVEFIADGVFKCQGEESGYKFPEVNLDDGEWFDYDEKAGEEVSITEVKWE
FVKA"
gene complement(<62521..>65175)
/gene="PMT1"
/locus_tag="AWJ20_3725"
/db_xref="GeneID:30035778"
mRNA complement(<62521..>65175)
/gene="PMT1"
/locus_tag="AWJ20_3725"
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT1"
/transcript_id="XM_018880749.1"
/db_xref="GeneID:30035778"
CDS complement(62521..65175)
/gene="PMT1"
/locus_tag="AWJ20_3725"
/inference="similar to AA sequence:KEGG_Orthology:K00728"
/codon_start=1
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT1"
/protein_id="XP_018733408.1"
/db_xref="GeneID:30035778"
/translation="MDVHPPLAKMLYAVVGWFAGYDGEFEFSDIGVEYTTSENYVPYI
AMRTFPAVLGVLTIMLSYSTLRASGVKPLIALFGSALMTFDNALATQSRYILLDSPLV
FFIAITAYGFKKFENEVPFTKDWYRYLFLTGLGLGATVSSKWVGLFTIGWVGALTLYQ
LWWLLGDLNVSYKKFLAHFGARVVFLIIVPIVFYVGMFALHFICLSNTGDGASFMSPE
FQSTLQHTNLAHDIPADVAFGSEIALRHWNTQGGYLHSHDHMYATGSKQQQVTLYPHK
DHNNAWVLENATALEEYETDAHGNITATNYLKPPVFIKDGAIVKLRHKSTFRRLHSHD
VRPPVSEQEHHNEVSAYGYEGFDGDANDNFRVEILEKYSEPGVARERVRTLQTKFRLV
HTMTGCHLFSHAVKLPKWAFEQQEVTCIKGGTLPNTIWYVEDNVNAELGPDAELVSYR
KPSFFEKFIELNKVMWKVNAGLTDTHAWQSRPESWPLMQRGINFWVKDYRQVYLIGNG
VTWWTLSLLVVLYVSYKAFWVLSWQRNVIQFYNDPDYALYDSNVGSYLLGWAFHYFPS
FLMDRQLFLHHYLGSVFFGILALAQAWQFLTTRLIRNKLVGHALTVIFFSGALAWYIW
YSPLIYGLPWTKDLCNQSKFFDMDFSCDSFHGTYAEYDIITRKRSVHYNEKSSSSSPE
VAESVAAPEEAAAQNVEPVAQPNESSGIIEQPEYKEYDYDVENPSPVENPIPSETAED
EDNLRESSNDGEGVSEFEAAKSEAPSEFETTPAPVIKESPVSFTRTLKKITYRDEDGN
PIDRAAAEKLIEKYKKGAVSPAPAAPAAGSPAPEPESSEPAAEPAAEPVPEPVPEPEP
VEPAVEEAVVPEDVPDNVPEEETGAAEV"
gene <70237..>71922
/gene="HOL1"
/locus_tag="AWJ20_3726"
/db_xref="GeneID:30035779"
mRNA <70237..>71922
/gene="HOL1"
/locus_tag="AWJ20_3726"
/product="Hol1p"
/transcript_id="XM_018880750.1"
/db_xref="GeneID:30035779"
CDS 70237..71922
/gene="HOL1"
/locus_tag="AWJ20_3726"
/note="Putative transporter in the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; mutations in membrane-spanning domains permit
cation and histidinol uptake; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005886 - plasma
membrane [Evidence ISS] [PMID 8955402]; GO_function:
GO:0015665 - alcohol transmembrane transporter activity
[Evidence IMP] [PMID 2405251]; GO_function: GO:0022890 -
inorganic cation transmembrane transporter activity
[Evidence IMP] [PMID 8955402]; GO_process: GO:0006812 -
cation transport [Evidence IMP] [PMID 8955402];
GO_process: GO:0015850 - organic hydroxy compound
transport [Evidence IMP] [PMID 2405251]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Hol1p"
/protein_id="XP_018733409.1"
/db_xref="GeneID:30035779"
/translation="MGLLYFPRNDTHLDDGTEIPGTVKLVDVDHNLDVQHQGEKDVIL
VPQPTNSPDDPLNWTPFRKIITLIWPCYYTFMVAVLAAVPAISYPEMVTDLGIPLGNL
VSGTGYLFLFFGLGCLFWQPIALNFGRRPVYIYTLLIGGIACPLWLNHVSSSGEYYAN
RIICGFFLSSVESLGEVVVGDLYFTHQRGMGINIYMMSLLSGTFLGPLAGSFITGSMT
WKWLNNFLAIFCGMGVILSYIFMEETMFFRPAAAEDILSRAGVVHHAEEQEADMSTTS
SSNGNEKNVASTVQLNSGAGSIEELAPPARKTYFQRYPILFYRSPGQPNLFFQSVIRP
LQIFVYFLPVAWSGLVVSLALAWWSVAGGTIAAILAEPPYNFGSSALGLVYISPLVSS
IICGYIGGPLSDRFCLWMARHNNGVREPEFRFYIGILALILTPAGLWMYGIGAAHQAP
WPVIVIGYGFSTVGSTLGQSLPYTYIMDCYPRIGGDSMVTVILVRNIIGGFAFAYAIT
PWIATDGYQTTFIILGLLSIFFWLTCIPIIIFGKSLRRKTAQRYHQYAQTSSH"
gene <75187..>76836
/gene="MNN10"
/locus_tag="AWJ20_3727"
/db_xref="GeneID:30035780"
mRNA <75187..>76836
/gene="MNN10"
/locus_tag="AWJ20_3727"
/product="Mnn10p"
/transcript_id="XM_018880751.1"
/db_xref="GeneID:30035780"
CDS 75187..76836
/gene="MNN10"
/locus_tag="AWJ20_3727"
/inference="similar to AA sequence:KEGG_Orthology:K05531"
/note="Subunit of a Golgi mannosyltransferase complex;
complex mediates elongation of the polysaccharide mannan
backbone; membrane protein of the mannosyltransferase
family; other members of the complex are Anp1p, Mnn9p,
Mnn11p, and Hoc1p; GO_component: GO:0005794 - Golgi
apparatus [Evidence IEA,IEA]; GO_component: GO:0000136 -
alpha-1,6-mannosyltransferase complex [Evidence IDA] [PMID
10037752]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_function: GO:0000009 -
alpha-1,6-mannosyltransferase activity [Evidence IDA]
[PMID 10037752]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_function: GO:0016757 -
transferase activity, transferring glycosyl groups
[Evidence IEA]; GO_function: GO:0016758 - transferase
activity, transferring hexosyl groups [Evidence IEA];
GO_process: GO:0000917 - barrier septum assembly [Evidence
IMP] [PMID 16034811]; GO_process: GO:0000032 - cell wall
mannoprotein biosynthetic process [Evidence IMP] [PMID
10037752]; GO_process: GO:0006487 - protein N-linked
glycosylation [Evidence IMP] [PMID 23210626]; GO_process:
GO:0006487 - protein N-linked glycosylation [Evidence IMP]
[PMID 8991513]"
/codon_start=1
/product="Mnn10p"
/protein_id="XP_018733410.1"
/db_xref="GeneID:30035780"
/translation="MRLPQAAFLACASFAILATIWYMATFSTVDATKWKLPSSVGNSK
PNKVKIQKTATAPVDPILLPPKTKTLAKVPIPTTTTEVLTDTEMQIERLQEALRSKDK
KIADSVQQLKDKDRLYNEEQEKKDKEVAAKLDALKKAQASLNDQKEKVQKEKEEERKK
KEEEERKKKELEEEKKKKEEEEKKKEEEDRKSLTDAYYIIRDRMLLEEHKLLEPIEIP
DEERHNHYDKDNKLMPNGKKILLLSAFDGAGSTDNDLTDLFMSNREEYANYHGYTHLF
VNTTKYKKNDKLKRPAVWFKLPAIKEAFEKFPEVEWIWWVDTDIIIMNEEIDLAAHVL
NPYVLSNRLTYERPVNDLGDHFSGSLYPARDHVDVDNIDIIFTQDHLGINAGSFFIKR
SWFAEFVLDMWGDKFLIESKFPREEQDAFIYLFTNHKTVFHHTGLVPQRMMNAYHGYY
LHTANWQEGDLCIHFAGHNKGLGFEKNFAHYWGLRKRVPKEFQINVPEEEAKLKVIAD
KAQKEKEEQERQKKEKEEQEKKEKEEKEKQEKEEKEKNGKQ"
gene <78829..>81855
/gene="FPK1"
/locus_tag="AWJ20_3728"
/db_xref="GeneID:30035781"
mRNA <78829..>81855
/gene="FPK1"
/locus_tag="AWJ20_3728"
/product="serine/threonine protein kinase FPK1"
/transcript_id="XM_018880752.1"
/db_xref="GeneID:30035781"
CDS 78829..81855
/gene="FPK1"
/locus_tag="AWJ20_3728"
/inference="similar to AA sequence:KEGG_Orthology:K08286"
/codon_start=1
/product="serine/threonine protein kinase FPK1"
/protein_id="XP_018733411.1"
/db_xref="GeneID:30035781"
/translation="MHRNRSRAMTVPSRPMNGSSPSIVGSINPGGDFESGLNGNGNSN
GFRVVSAGSSSSSSNLPQKLKNLFRLSSFSNSNSVGGESSGSGAGHLTTGTHHTGSGT
ELSSGNPNTTDSSPAIGSENTPELSLAHTGDDADASDSPDTPNPVANDSTNWGFKSSP
GPSDILAKPPKRSFLGRLRSPSSPSPMLLTPDPEEGQESSPPPLPKGLSVSAQASPSL
RPVNAPSDTVASANPYFSYQGQPAHAQQREEQQQHLHQGHGQAQKAQLQHSQQHYSSN
QHQNDHQHPNQNLHQQHQQQSQPTQNTSRPQNRRNNSHSLFSFSSFLGDHSNSNHNHG
RNTNTNKELASSTNDENNGASDLKSDSTVSAGSDGGYIVNGANHSTTNASTPLAENDI
TIVALPAEDHEEPLAPPSKGIFSRNLRRVASAPNEIKNMLLNSPQVRPADGPSGTIPS
GGINGTGNQASSNTNTSDNATAIGADNSRPDQGTGISSHSISTSNNGLKANKSSSTGL
TTTTFSKARSPSTPKVYNRSYSRSSIKICDVEVTPSSFDKIKLLGKGDVGKVYLVREK
KTNHLYAMKVLSKREMIKRNKIKRALAEQEILATSNHPFIVTLYHSFQSDEHLYLCME
YCMGGEFFRALQTREGKSLKEDDARFYAAEVTAALEYLHLMGYIYRDLKPENILLHQS
GHIMLSDFDLSKQSGSEAAPTIVNGSGRSGGGSSSNLPAIDTKACIADFRTNSFVGTE
EYIAPEVIRGNGHTSAVDWWTLGILLFEMLFGTTPFKGKNRNATFANILKSDVNFYDV
QPVSSNCKSIIRKLLIKDENKRLGSRAGASDIKGHPFFKTTQWALLRNRVPPIIPVQG
KGRDAVNFRKMKESGSVDFDEETPYDANANSTHSSAGDPSEPSNFAPDPFSNFNSITL
HHDGGDADGNAYYDDSDAEEEETTTAAPSGRQSPLANPSSTHTSVPGSPKVYNGFGSD
METDISTHHSVFSGAGSSSPSLFKPYFKGFTTSLKN"
gene complement(<81989..>83152)
/gene="FEX2"
/locus_tag="AWJ20_3729"
/db_xref="GeneID:30035782"
mRNA complement(<81989..>83152)
/gene="FEX2"
/locus_tag="AWJ20_3729"
/product="Fex2p"
/transcript_id="XM_018880753.1"
/db_xref="GeneID:30035782"
CDS complement(81989..83152)
/gene="FEX2"
/locus_tag="AWJ20_3729"
/note="Protein involved in fluoride export; nearly
identical to FEX1, and deletion of both proteins results
in a large increase in fluoride sensitivity compared with
the single mutant; contains two FEX domains connected by a
linker; part of a widespread family of conserved fluoride
export proteins; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA,IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008150 -
biological_process [Evidence ND]"
/codon_start=1
/product="Fex2p"
/protein_id="XP_018733412.1"
/db_xref="GeneID:30035782"
/translation="MDEVAESSDPSTGSSVRGHVEPKGEEQYRRFRRHVFVTVISNKY
IDVVVLHLLLAFFGITGVLARVGLTRLTSFPGSQNGGVLWSNFGGCLLMGILVRSEAL
FGDLIVIEQMAPLDVVEHGASGNVLRRTYTRKEKIPLYIGLATGVCGSITSFSTFMIE
LFQLSALQPPTGVSYPNPGYGVMAFLSYLIITLTVSFGGYFCGFHVADGIEKFFPISL
ATYTLIIEVLGAMLGLTAWVAVIVLAILIPQWRYWTFACIFGPFGVYARFWSARLFNK
ITHKFLVGTFIANMIATAILCALIILQRGQTPSHGLIIKSKLQCQAISALQDGFCGNF
STISSFIAELVAFRSKRNAYIYGGISIGGGFSIVVVILGSYAWTRGISAVAAC"
gene <84401..>84823
/gene="GCN3"
/locus_tag="AWJ20_3730"
/db_xref="GeneID:30035784"
mRNA <84401..>84823
/gene="GCN3"
/locus_tag="AWJ20_3730"
/product="translation initiation factor eIF2B subunit
alpha"
/transcript_id="XM_018880755.1"
/db_xref="GeneID:30035784"
CDS 84401..84823
/gene="GCN3"
/locus_tag="AWJ20_3730"
/inference="similar to AA sequence:KEGG_Orthology:K03239"
/note="Alpha subunit of translation initiation factor
eIF2B; guanine-nucleotide exchange factor for eIF2;
activity subsequently regulated by phosphorylated eIF2;
positive regulator of GCN4 expression; assembles into
filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells
approach stationary phase and under cytosolic
acidification and starvation conditions; GO_component:
GO:0005851 - eukaryotic translation initiation factor 2B
complex [Evidence IMP] [PMID 8099443]; GO_component:
GO:0005851 - eukaryotic translation initiation factor 2B
complex [Evidence IDA] [PMID 8336705]; GO_component:
GO:0005851 - eukaryotic translation initiation factor 2B
complex [Evidence IDA,IPI] [PMID 8506384]; GO_component:
GO:0032045 - guanyl-nucleotide exchange factor complex
[Evidence IDA] [PMID 8506384]; GO_function: GO:0030234 -
enzyme regulator activity [Evidence IMP] [PMID 8099443];
GO_function: GO:0030234 - enzyme regulator activity
[Evidence IGI,IMP,IPI] [PMID 8887689]; GO_function:
GO:0030234 - enzyme regulator activity [Evidence IMP]
[PMID 9032257]; GO_function: GO:0005085 -
guanyl-nucleotide exchange factor activity [Evidence IDA]
[PMID 8506384]; GO_function: GO:0003743 - translation
initiation factor activity [Evidence IEA]; GO_function:
GO:0003743 - translation initiation factor activity
[Evidence IMP] [PMID 8099443]; GO_function: GO:0003743 -
translation initiation factor activity [Evidence IDA]
[PMID 8336705]; GO_process: GO:0044237 - cellular
metabolic process [Evidence IEA]; GO_process: GO:0006417 -
regulation of translation [Evidence IEA]; GO_process:
GO:0006446 - regulation of translational initiation
[Evidence IMP] [PMID 8099443]; GO_process: GO:0006446 -
regulation of translational initiation [Evidence IDA]
[PMID 8506384]; GO_process: GO:0006446 - regulation of
translational initiation [Evidence IGI,IPI] [PMID
8887689]; GO_process: GO:0006446 - regulation of
translational initiation [Evidence IMP] [PMID 9032257];
GO_process: GO:0006412 - translation [Evidence IEA];
GO_process: GO:0006413 - translational initiation
[Evidence IEA]"
/codon_start=1
/product="translation initiation factor eIF2B subunit
alpha"
/protein_id="XP_018733413.1"
/db_xref="GeneID:30035784"
/translation="MARILRENGIPVCQIPDSAVGYVIDKVDKIFFGAEGVAESGGVI
NQMGSYQIAVLAKTTNTPVYVLAESHKFVRLYPLSPDDLPTEYPLEFTTKVDGERDIS
ESPQVDFTPHQYVTALITDLGTLTPSGVSEELIKIWFD"
gene complement(<85030..>85836)
/gene="RPC37"
/locus_tag="AWJ20_3731"
/db_xref="GeneID:30035785"
mRNA complement(<85030..>85836)
/gene="RPC37"
/locus_tag="AWJ20_3731"
/product="Rpc37p"
/transcript_id="XM_018880756.1"
/db_xref="GeneID:30035785"
CDS complement(85030..85836)
/gene="RPC37"
/locus_tag="AWJ20_3731"
/inference="similar to AA sequence:KEGG_Orthology:K14721"
/note="RNA polymerase III subunit C37; GO_component:
GO:0005666 - DNA-directed RNA polymerase III complex
[Evidence IDA] [PMID 10611227]; GO_component: GO:0005666 -
DNA-directed RNA polymerase III complex [Evidence IDA]
[PMID 3905793]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA,IEA]; GO_function: GO:0003899 -
DNA-directed RNA polymerase activity [Evidence IEA,IEA];
GO_function: GO:0001056 - RNA polymerase III activity
[Evidence IDA] [PMID 3905793]; GO_process: GO:0042797 -
tRNA transcription from RNA polymerase III promoter
[Evidence IDA] [PMID 3905793]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Rpc37p"
/protein_id="XP_018733414.1"
/db_xref="GeneID:30035785"
/translation="MAAEDEETGLFMDDNESDDMAAVAESDVDMKDEEDPVSEAKQDA
GLDDEDDPVVREFPVYSSQVLGKKLHILQYPTRSALRPLVAAQGTGIINSRLKPKSGI
IEVDIPIDTSKFYDAEKGDRWNKVDRQTFSGVLKKGQGRYMAGVFRDGELHVSSIESV
AQLRPQFKYFDKHVNSEKEANRMIRTDPTKPREARTIQMSAKSSGDFAPKYSGALGAR
KIADEESFVDIQWFDRDSNESWDMADKFVTTNKRILDSSTTLKDYTDKLQ"
gene <86579..>88897
/gene="DBP7"
/locus_tag="AWJ20_3732"
/db_xref="GeneID:30035786"
mRNA <86579..>88897
/gene="DBP7"
/locus_tag="AWJ20_3732"
/product="Dbp7p"
/transcript_id="XM_018880757.1"
/db_xref="GeneID:30035786"
CDS 86579..88897
/gene="DBP7"
/locus_tag="AWJ20_3732"
/inference="similar to AA sequence:KEGG_Orthology:K14806"
/note="Putative ATP-dependent RNA helicase of the DEAD-box
family; involved in ribosomal biogenesis; required at
post-transcriptional step for efficient
retrotransposition; essential for growth under anaerobic
conditions; GO_component: GO:0005730 - nucleolus [Evidence
IEA]; GO_component: GO:0005730 - nucleolus [Evidence IDA]
[PMID 9582098]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0004004 -
ATP-dependent RNA helicase activity [Evidence ISS] [PMID
10322435]; GO_function: GO:0008026 - ATP-dependent
helicase activity [Evidence IEA]; GO_function: GO:0003723
- RNA binding [Evidence IEA]; GO_function: GO:0004386 -
helicase activity [Evidence IEA,IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0003676 - nucleic acid binding [Evidence
IEA]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_process: GO:0006200 - ATP catabolic
process [Evidence IEA]; GO_process: GO:0000463 -
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
9582098]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA]"
/codon_start=1
/product="Dbp7p"
/protein_id="XP_018733415.1"
/db_xref="GeneID:30035786"
/translation="MSDDGMLLNFSTEPSTSAVRKPVGSKVQGGRWKDRLNAKRAQVK
QYEKENPEVRVARVAKERAIKEKAEIVRQKRTAGRNGGKKPPGTGTSEASGSGAGAGA
GSGSGSNGSSRARMTNFKKPTAAQQKQHPGVNLNPEDKNTFVSSLFSSNPSLAENDAE
DKPVESHEASNAPLKDDTTFEGLGVNSALTLQLSTKMKIPTPTRIQRAALPPLISSNR
DLFIQAQTGSGKTLAYALPIIHRLMSTPDLTRESGLFALILTPTRELSTQIYSVLEKL
VGACHWLVPGIVIGGEKKKSEKARIRKGINILVATPGRLCDHFDNTESLDLSQIRWVV
LDEGDRLMELGFEEAITKILTKIGKTSMIHDTAKRWPQLPNKRVNILCSATMRDDVKR
LGELSLSHAEWVTPDSVGEDSKKHLRSDANDNHEHIAPAQLIQQSAIVPAKLRLVTLA
GLLKAITSAEKGSRTIVFFSCSDSVDFHFKAFTKGGTNRLKQRTSNNHDSDDEDDTSS
ATQPTVMTSVDLDNTVIHKLHGSLNQQLRTSTLSSFSAKDGAANGESYILFCTDVASR
GLDLPHITQVIEYDPPFGMDDHLHRVGRTARAGKEGTSILFLLPGAEEKYLDVIKPNH
PNGIHPRSYEDILKKGFGSKWDVDATTWHLNIERWLLEDASALQLAKRGYTSHIRAYT
THLSSEREIFNLRALHLGHLAKSFALRETPGKMSKQPSSKTDKTKPSSKKTKSSSGVA
KPQPRGDADGNRRRMLAVAKSHAGMSEFNIGY"
gene <93699..>95492
/locus_tag="AWJ20_3733"
/db_xref="GeneID:30035787"
mRNA <93699..>95492
/locus_tag="AWJ20_3733"
/product="hypothetical protein"
/transcript_id="XM_018880758.1"
/db_xref="GeneID:30035787"
CDS 93699..95492
/locus_tag="AWJ20_3733"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733416.1"
/db_xref="GeneID:30035787"
/translation="MATLIPQFPVGNNSNHNTSGTSAPSENQILARLDAYLPALNTGL
MDGKIQDVIIKHPLVRTLLVPECDLEARRRCIALISKALAAATPVLPLILQDHQAKQD
QKKKRQKSNAGSRKADANAQADSPRIPKLSLDAQSRLITIVASMAERDPLIRFWMNHH
VQYQARSQAQAQAQSATPEEGSRKRKYSTPAGSGAAAAAAASSAVGGSGMSKHLEMST
FDLYLKIKTSKELLADLADRIQGAVCKAFWDCWDEIFITLAEVIGAENLQQADKGRIF
RPCGDAAIYLNNLKVTKKRQVEQKRRLSMLEGNTSANTRPGHQAFYMGNNNMTTVSPK
NILGQQPGASPIQVTGQIPSQFSSQNQNIPPLVPASPFSPDQFLNEFTLNSSIPGTTA
TTTAAASSIPTPSASGASMAFPFPLSPEKSSGSPDSTSSMILGINTASAVSNVPTPPG
STNGSTSSHPNPFNIASASTSVNGSKPLSGSDANDLEFMMQDISYDSKFDQYLGAFTT
LDDADQQSGHQANPSHTPSSNTAANSSTSPAATTKPLPSNASDISTPQTSAGSPTNTP
GGSHLNLRQRIKLMEEGRMPEENPSPSNIVW"
gene <98087..>100627
/locus_tag="AWJ20_3734"
/db_xref="GeneID:30035788"
mRNA <98087..>100627
/locus_tag="AWJ20_3734"
/product="hypothetical protein"
/transcript_id="XM_018880759.1"
/db_xref="GeneID:30035788"
CDS 98087..100627
/locus_tag="AWJ20_3734"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733417.1"
/db_xref="GeneID:30035788"
/translation="MSSSIAASGGSSRTFSKSELDHSTSSYAVLGTSPISDGGLLNSK
GFGPENGQQSRINMEGSNQRGENYDPRKTVNDGGTTVTYKLHKSCDYCRHRKIKCVRR
PGSEICDHCRICEVECTFSRKMPSKKRKLRSARIVASLRDRDRLGYEGSGIGSADDTN
DSMGMSIRGRGSVSEEAVDNGNFIAKEESTASPKDRNHLTAVKIEPESADSEVGSGEG
AIGNATVPGVPSSVKGVSVSTPIMSYTQQGTGMTPLTAGGWYYEPFFDLIYSAKASPM
EDPSNGKSGAPSPLPAESVNEESSAFPQASEGLQGNSKTDVFAIHGHEVHSTNHVIPT
TNHGVRGDHDDTHANNRKQTENNYPSINGSTDSPHSQYTGSPNIAEMALSGYASRPNT
ETNSNNNINTNGNANTSSNEPISASVSVTTPGGTAHALPLDNNSNLTSLAIIATEHNA
SLTVPSNHSRRLPEDPQAVRDAYTLYIEPCTPFFPPEFYDASDMSTLARCCMSLASFS
SPNSVAPVGSIAMLYEVVDQLVAQETVWNEFSLSAVFCVLVRYKLPYDRVSRAINQFI
KKGPQHYPSPGINYNLLIGALSAYAWNSLVAYDNLRPPNNDEAEIIDIYETVTSNISE
DTFAYHFLYVSTLLCRFKVLMILPDEVYSPESLASGSPQHDDAWYSTRKSLIRLEYEL
LLWPVRLPKHLTVVKDDLIATPGALILHVLHNTVLLAYYIQAIRHKDPYGRMLSILPV
PGLLQFLCGMAASSFKCQPDIIEKWPIIRDCLVITARLMLELYNQTEYENCKVALALY
QDSQTAPGLYNSIQKVLAAVDWAANDKDGAALYWIFRDVRSMSLQHLL"
gene complement(<101761..>102183)
/gene="HHT2"
/locus_tag="AWJ20_3735"
/db_xref="GeneID:30035789"
mRNA complement(<101761..>102183)
/gene="HHT2"
/locus_tag="AWJ20_3735"
/product="histone H3"
/transcript_id="XM_018880760.1"
/db_xref="GeneID:30035789"
CDS complement(101761..102183)
/gene="HHT2"
/locus_tag="AWJ20_3735"
/inference="similar to AA sequence:KEGG_Orthology:K11253"
/note="Histone H3; core histone protein required for
chromatin assembly, part of heterochromatin-mediated
telomeric and HM silencing; one of two identical histone
H3 proteins (see HHT2); regulated by acetylation,
methylation, and phosphorylation; H3K14 acetylation plays
an important role in the unfolding of strongly positioned
nucleosomes during repair of UV damage; GO_component:
GO:0043505 - centromere-specific nucleosome [Evidence IDA]
[PMID 22693454]; GO_component: GO:0005694 - chromosome
[Evidence IEA,IEA]; GO_component: GO:0000788 - nuclear
nucleosome [Evidence TAS] [PMID 2275823]; GO_component:
GO:0000786 - nucleosome [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 11914276];
GO_component: GO:0031298 - replication fork protection
complex [Evidence IDA] [PMID 16531994]; GO_function:
GO:0003677 - DNA binding [Evidence IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence TAS] [PMID 2275823];
GO_function: GO:0046982 - protein heterodimerization
activity [Evidence IEA]; GO_process: GO:0006281 - DNA
repair [Evidence IEA]; GO_process: GO:0006974 - cellular
response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0006333 - chromatin assembly or disassembly
[Evidence TAS] [PMID 2275823]; GO_process: GO:0070911 -
global genome nucleotide-excision repair [Evidence IMP]
[PMID 21460225]; GO_process: GO:0007094 - mitotic spindle
assembly checkpoint [Evidence IGI] [PMID 19917722];
GO_process: GO:0006334 - nucleosome assembly [Evidence
IEA]; GO_process: GO:0009303 - rRNA transcription
[Evidence IMP] [PMID 16002464]; GO_process: GO:0043935 -
sexual sporulation resulting in formation of a cellular
spore [Evidence IMP] [PMID 20713519]"
/codon_start=1
/product="histone H3"
/protein_id="XP_018733418.1"
/db_xref="GeneID:30035789"
/translation="MARTKSTVARKSTGGKAPRKQIASKAARKSAPSTAAPGGVKKPH
RYKPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVE
AYLVSLFEDTNLCAIHAKRVTIQKKDIQLARRLRGERI"
gene <103937..>104248
/gene="HHF2"
/locus_tag="AWJ20_3736"
/db_xref="GeneID:30035790"
mRNA <103937..>104248
/gene="HHF2"
/locus_tag="AWJ20_3736"
/product="histone H4"
/transcript_id="XM_018880761.1"
/db_xref="GeneID:30035790"
CDS 103937..104248
/gene="HHF2"
/locus_tag="AWJ20_3736"
/inference="similar to AA sequence:KEGG_Orthology:K11254"
/note="Histone H4; core histone protein required for
chromatin assembly and chromosome function; one of two
identical histone proteins (see also HHF2); contributes to
telomeric silencing; N-terminal domain involved in
maintaining genomic integrity; GO_component: GO:0005694 -
chromosome [Evidence IEA,IEA]; GO_component: GO:0000788 -
nuclear nucleosome [Evidence TAS] [PMID 2275823];
GO_component: GO:0000786 - nucleosome [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_component: GO:0031298 - replication fork protection
complex [Evidence IDA] [PMID 16531994]; GO_function:
GO:0003677 - DNA binding [Evidence IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence TAS] [PMID 2275823];
GO_function: GO:0046982 - protein heterodimerization
activity [Evidence IEA]; GO_process: GO:0006333 -
chromatin assembly or disassembly [Evidence TAS] [PMID
2275823]; GO_process: GO:0034729 - histone H3-K79
methylation [Evidence IMP] [PMID 18158898]; GO_process:
GO:0006334 - nucleosome assembly [Evidence IEA];
GO_process: GO:0043935 - sexual sporulation resulting in
formation of a cellular spore [Evidence IMP] [PMID
20713519]; GO_process: GO:0061587 - transfer RNA
gene-mediated silencing [Evidence IMP] [PMID 23707796]"
/codon_start=1
/product="histone H4"
/protein_id="XP_018733419.1"
/db_xref="GeneID:30035790"
/translation="MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGV
KRISALIYEETRGVLKSFLESVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFG
G"
gene <104583..>105596
/gene="RKI1"
/locus_tag="AWJ20_3737"
/db_xref="GeneID:30035791"
mRNA <104583..>105596
/gene="RKI1"
/locus_tag="AWJ20_3737"
/product="ribose-5-phosphate isomerase RKI1"
/transcript_id="XM_018880762.1"
/db_xref="GeneID:30035791"
CDS 104583..105596
/gene="RKI1"
/locus_tag="AWJ20_3737"
/inference="similar to AA sequence:KEGG_Orthology:K01807"
/note="Ribose-5-phosphate ketol-isomerase; catalyzes the
interconversion of ribose 5-phosphate and ribulose
5-phosphate in the pentose phosphate pathway; participates
in pyridoxine biosynthesis; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0016853 - isomerase activity [Evidence
IEA]; GO_function: GO:0004751 - ribose-5-phosphate
isomerase activity [Evidence IEA,IEA]; GO_function:
GO:0004751 - ribose-5-phosphate isomerase activity
[Evidence IMP,ISS] [PMID 8929392]; GO_process: GO:0006098
- pentose-phosphate shunt [Evidence IEA]; GO_process:
GO:0006098 - pentose-phosphate shunt [Evidence IGI] [PMID
8929392]; GO_process: GO:0009052 - pentose-phosphate
shunt, non-oxidative branch [Evidence IEA]; GO_process:
GO:0008615 - pyridoxine biosynthetic process [Evidence
IMP] [PMID 14690456]"
/codon_start=1
/product="ribose-5-phosphate isomerase RKI1"
/protein_id="XP_018733420.1"
/db_xref="GeneID:30035791"
/translation="MYGRALGLGQGGGGARAPLSRIGRTPAGLVRPGACAASGLVVSR
AGGCGEGLGVKDVSSGGKRPSSIQRSLLHRLRPGPSCVFIRSMSLRLGEISLVEKAKR
AAAYKAVDEHFPADAKVVGIGSGSTVIYAVERIAQLAELRGIDLSKVTFVPTGFQSKQ
LIIGAGLNVASIDQFSVGDLDIVIDGADEVDDSLNCIKGGGACLFQEKLVGQCARKFV
IVADSSKKSHKLGSKWIQGVPIEVIPMAYPKVEADLLRLGAKQITLRQGGKAKAGPVV
TDNGNFILDTHFGSIEPSEVRNLDIKIKLLVGVVETGLFDYGAVAYFGETDGTASTFT
RPE"
gene complement(<108260..>109582)
/gene="APM1"
/locus_tag="AWJ20_3738"
/db_xref="GeneID:30035792"
mRNA complement(<108260..>109582)
/gene="APM1"
/locus_tag="AWJ20_3738"
/product="Apm1p"
/transcript_id="XM_018880763.1"
/db_xref="GeneID:30035792"
CDS complement(108260..109582)
/gene="APM1"
/locus_tag="AWJ20_3738"
/inference="similar to AA sequence:KEGG_Orthology:K12393"
/note="Mu1-like medium subunit of the AP-1 complex; binds
clathrin; involved in clathrin-dependent Golgi protein
sorting; the AP-1 complex is the clathrin-associated
protein complex; GO_component: GO:0030121 - AP-1 adaptor
complex [Evidence IPI,ISS] [PMID 10564262]; GO_component:
GO:0030131 - clathrin adaptor complex [Evidence IEA];
GO_component: GO:0030665 - clathrin-coated vesicle
membrane [Evidence IEA]; GO_component: GO:0005905 - coated
pit [Evidence IEA,IEA]; GO_component: GO:0016023 -
cytoplasmic membrane-bounded vesicle [Evidence IEA];
GO_component: GO:0031410 - cytoplasmic vesicle [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0030276 - clathrin binding [Evidence
IPI,ISS] [PMID 10564262]; GO_process: GO:0006896 - Golgi
to vacuole transport [Evidence IMP] [PMID 17003107];
GO_process: GO:0006886 - intracellular protein transport
[Evidence IEA]; GO_process: GO:0015031 - protein transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA,IEA]; GO_process: GO:0016192 -
vesicle-mediated transport [Evidence IEA]; GO_process:
GO:0016192 - vesicle-mediated transport [Evidence IPI,ISS]
[PMID 10564262]"
/codon_start=1
/product="Apm1p"
/protein_id="XP_018733421.1"
/db_xref="GeneID:30035792"
/translation="MASAIFFLDLKGKHLLSRNYRGDIPMSAVDKFPILLMEAEEETS
IVPPTISHEGVNYLYIQHNNLYLLALTKRNSNAAQILLFLHKIVEVLTEYFKELEEES
IRDNFVVIYELLDEMMDFGYPQTTDTKILQEYITQESHALDVQARPPVAVTNAVSWRS
EGITYRKNEAFLDVVESVNLLISASGQVLRSEILGSVQMKCFLSGMPELRLGLNDKVI
FNHQSNTTGQSIGSSSGGGSSGKVIEMEDVKFHQCVRLSRFENDRTISFIPPDGEFEL
MSYRLNTNVKPLILVDCRVTNFSSSRIEIIVKARAQFKKRSNANNVEITIPVPDDADS
PKFKTTAGNVKWHPEKSAIIWRIKQFGGGREFNMRAELGLPSVKDSDYANAKIKPPIT
VNFTIPYFTTSGIQVRYLKIIEPKLQYQSYPWVRYITKSGNDYTIRMS"
gene <108522..>108920
/locus_tag="AWJ20_3739"
/db_xref="GeneID:30035793"
mRNA <108522..>108920
/locus_tag="AWJ20_3739"
/product="hypothetical protein"
/transcript_id="XM_018880764.1"
/db_xref="GeneID:30035793"
CDS 108522..108920
/locus_tag="AWJ20_3739"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733422.1"
/db_xref="GeneID:30035793"
/translation="MMADFSGCHFTFPAVVLNLGESASSGTGIVISTLLAFDRFLNCA
RALTMISIRDEEKLVTRQSTRISGLTLVFRRYDMSSNSPSGGINEIVRSFSNRDRRTH
WWNLTSSISMTFPDEPPPEDDPMLCPVVLD"
gene complement(<111418..>113187)
/locus_tag="AWJ20_3740"
/db_xref="GeneID:30035795"
mRNA complement(<111418..>113187)
/locus_tag="AWJ20_3740"
/product="hypothetical protein"
/transcript_id="XM_018880766.1"
/db_xref="GeneID:30035795"
CDS complement(111418..113187)
/locus_tag="AWJ20_3740"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733423.1"
/db_xref="GeneID:30035795"
/translation="MVKIALAPAPPQPLASKRPVAAGTVPQNNVPRIAAKKPLVAPSP
RAVLSAAASNAVSSIPTNNPVKPVLTTASALASSTRSRTSVSAASPVAVSSPTATAAS
SPVDTTGAAPVSKISTSKVWVLPPRPKPGRKPSVDTPQNKRKAQNRAAQRAFRERRAA
MVGELEEKLNELTKERDAREARLEAQLKAVTRENENLKRSVRDMQAQLAKQNVSYQMA
SPPMSSHESPMDILDRVLEERLPVPDKSRTNSSSDACGVCIKDDCICESVGIKARRTS
NQPVSAVSMSTTRSSISSITGTSSAFKTNDQSNAAATLVASSVTAPAVSLKRNQPESN
DDEPYEIDFTEMFSRPAKKQAPPLLVPSPVPVPKHRTSSTRTVLKPPADRCGFCSDGN
PCMCAEAEAEAQAAGDGGHFINDIEEDNTLPPIVLNNMWSSSYQNTNKLPALHPGPSR
EVPPSQQPPSPQVALPAASVSSITAPSYECTGNPGTCMQCQTDPMSTLFCTTLASRPE
KGDSEKKKAVTTPSTPTEKTRAGSSSFISASAAYRTLSRHKDFRSADFGALVGKLNTR
GMQVEVNSVANVLRELDRKLYSS"
gene <115364..>117727
/gene="PET127"
/locus_tag="AWJ20_3741"
/db_xref="GeneID:30035796"
mRNA <115364..>117727
/gene="PET127"
/locus_tag="AWJ20_3741"
/product="Pet127p"
/transcript_id="XM_018880767.1"
/db_xref="GeneID:30035796"
CDS 115364..117727
/gene="PET127"
/locus_tag="AWJ20_3741"
/note="Protein with a role in 5'-end processing of
mitochondrial RNAs; located in the mitochondrial membrane;
GO_component: GO:0005740 - mitochondrial envelope
[Evidence IDA] [PMID 9111353]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 16823961];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0000964 - mitochondrial RNA 5'-end
processing [Evidence IMP] [PMID 18086665]; GO_process:
GO:0000964 - mitochondrial RNA 5'-end processing [Evidence
IMP] [PMID 9111353]"
/codon_start=1
/product="Pet127p"
/protein_id="XP_018733424.1"
/db_xref="GeneID:30035796"
/translation="MLRTGGLGSLRLRIYSEYAPKFLSPGNGLFYTRAFSLSSTVLKD
IDGLDSLHLLEKETYRDIKRQRPAKGKKSPPGPNTRPGSITPKHFGKKRPSRAPPSRV
TLGQREPGAKPTFRSRREYEKKNEEQVNRNRQNSSAKVPKPSSAEVQEYIAKSPEQWK
SRRTLTDPKRFSKDNAGSSSETTNQKISPANSPFPNFFPHETPYFRSFELDSSQLSYE
PLEVPDQPPVPKLAHNLDRVLFSPGVHVLKDRRSNVYNFTPYLENIMSIKDFDFDFIA
KYVPSGKDSILSKLAKENGKKYTGSTSSLTSVLSQFHHLLSHRRKPHTMALSKYFQSQ
QVAFSAIQTQPVSIFLRYNPNTDTHSIDSDKSYSGDIIVSVLGHQLEAMLTTEEEEFR
NYHKSLSHKLDSSKKGGLNTYNYTQSGDFLMRSQLDCYDERLPGTGTFDLKTRAVCAV
RHDMDYTQIHDGSDYQITKIDGEFESFSREWFELIRSTMFKYSLQARIGRMDGIFLAY
HNVRRMFGFQYVPLSEIDQIYHTSDLIESQQDLRDIDPSNVSSFVPLDEQIATSGPII
AENEFKLSLKMFNEILDKIVASHEPAASFNLVFHTVSTGRMQVFVKPMLEKDIEVIQE
TGCDTEPKTGEEYSNNSDPHTILMHGNKNPEVLPPDVKVYQVDIRTYVNDKFVPGDKY
PHLSSDSDVWKVQCEMSQLRQQGAERRFSRTFSMHKIENRITSVPQTERLDEEQEETI
RNEALSKLSPPNAKQGYLRFLGAKGERLQREWEAQHGHKEKHVWRPS"
gene complement(<117861..>118880)
/gene="THI73"
/locus_tag="AWJ20_3742"
/db_xref="GeneID:30035797"
mRNA complement(<117861..>118880)
/gene="THI73"
/locus_tag="AWJ20_3742"
/product="Thi73p"
/transcript_id="XM_018880768.1"
/db_xref="GeneID:30035797"
CDS complement(117861..118880)
/gene="THI73"
/locus_tag="AWJ20_3742"
/note="Putative plasma membrane permease; proposed to be
involved in carboxylic acid uptake and repressed by
thiamine; substrate of Dbf2p/Mob1p kinase; transcription
is altered if mitochondrial dysfunction occurs;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IDA] [PMID 16850348]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISS] [PMID 10869563];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence IEA,IEA]; GO_function: GO:0005215 -
transporter activity [Evidence ISS] [PMID 10869563];
GO_process: GO:0055085 - transmembrane transport [Evidence
IEA]; GO_process: GO:0006810 - transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence ISS] [PMID
10869563]"
/codon_start=1
/product="Thi73p"
/protein_id="XP_018733425.1"
/db_xref="GeneID:30035797"
/translation="MWYKKAEQGKRIGAFYVMNSVTLIAAGIISYAASFAKTAFASWR
IFLLCMGLVTVFCGICVFLFLPDSIVRSKGFTDEEKVAALLRVKDEQSGTQNSKLKRY
QMVEAVSDPKVWFVVLSVFLFSIPNGAITVFNSIIINSYGFGSQETLIVGAPAGVVTG
AAVIIIQYYSDKTQNRTLISIWYFIPSIVGLAIMIALGDREKTLSMKAGLLIASYLSQ
VFGGGLALFLSWNASNIAGHSKKALVNALTFIAFPLGNILGTQTFQNKDAPMYIPGKI
SIMACLCAQVVVSTVWFFVNKYYNKKKQTFLDTLNVYEYEGLRMKMEFSDETDMRNPF
FKYTK"
gene complement(<120325..>120753)
/gene="HHO1"
/locus_tag="AWJ20_3743"
/db_xref="GeneID:30035798"
mRNA complement(<120325..>120753)
/gene="HHO1"
/locus_tag="AWJ20_3743"
/product="histone H1"
/transcript_id="XM_018880769.1"
/db_xref="GeneID:30035798"
CDS complement(120325..120753)
/gene="HHO1"
/locus_tag="AWJ20_3743"
/inference="similar to AA sequence:KEGG_Orthology:K11275"
/note="Histone H1, linker histone with roles in meiosis
and sporulation; decreasing levels early in sporulation
may promote meiosis, and increasing levels during
sporulation facilitate compaction of spore chromatin;
binds to promoters and within genes in mature spores; may
be recruited by Ume6p to promoter regions, contributing to
transcriptional repression outside of meiosis; suppresses
DNA repair involving homologous recombination;
GO_component: GO:0005694 - chromosome [Evidence IEA,IEA];
GO_component: GO:0000790 - nuclear chromatin [Evidence
IDA] [PMID 22586276]; GO_component: GO:0000788 - nuclear
nucleosome [Evidence IPI] [PMID 11574687]; GO_component:
GO:0000786 - nucleosome [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 11914276];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
9046096]; GO_function: GO:0003677 - DNA binding [Evidence
IEA,IEA]; GO_function: GO:0003677 - DNA binding [Evidence
IPI] [PMID 11574687]; GO_process: GO:0030261 - chromosome
condensation [Evidence IMP] [PMID 22586276]; GO_process:
GO:0045910 - negative regulation of DNA recombination
[Evidence IGI,IMP] [PMID 12820979]; GO_process: GO:0031936
- negative regulation of chromatin silencing [Evidence
IGI] [PMID 19017647]; GO_process: GO:0006334 - nucleosome
assembly [Evidence IEA]; GO_process: GO:0006355 -
regulation of transcription, DNA-templated [Evidence IMP]
[PMID 11574687]"
/codon_start=1
/product="histone H1"
/protein_id="XP_018733426.1"
/db_xref="GeneID:30035798"
/translation="MVKDAILQLKERSGSSRQAIKKYIQSTHSISAANFDSLLNAAIR
KGVVSGDFVQPKGPSGPVKLQKKEVVKPAKKPVAKKTETKPAAKAKAKTTTKPKTVAK
KPAAKKTEKKPAVKATKAKATASKVKKAAPKKKATATKAK"
gene <126947..>128770
/gene="SEF1"
/locus_tag="AWJ20_3744"
/db_xref="GeneID:30035799"
mRNA <126947..>128770
/gene="SEF1"
/locus_tag="AWJ20_3744"
/product="Sef1p"
/transcript_id="XM_018880770.1"
/db_xref="GeneID:30035799"
CDS 126947..128770
/gene="SEF1"
/locus_tag="AWJ20_3744"
/note="Putative transcription factor; has homolog in
Kluyveromyces lactis; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_component: GO:0005634
- nucleus [Evidence IEA,IEA,IEA]; GO_function: GO:0003677
- DNA binding [Evidence IEA,IEA]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND];
GO_function: GO:0000981 - sequence-specific DNA binding
RNA polymerase II transcription factor activity [Evidence
IEA]; GO_function: GO:0008270 - zinc ion binding [Evidence
IEA]; GO_process: GO:0008150 - biological_process
[Evidence ND]; GO_process: GO:0006357 - regulation of
transcription from RNA polymerase II promoter [Evidence
IEA]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA,IEA];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA,IEA]"
/codon_start=1
/product="Sef1p"
/protein_id="XP_018733427.1"
/db_xref="GeneID:30035799"
/translation="MKAKACTECRQNKLRCLYPPSGIGSCQRCFKKELACVFDPKFRR
VSKRKLAERKSATGSAESEIRSNGVSSSSSTTNLNPSIPSSAPPLHSIAITQDGPTGE
PPKTSLDILDMPEQLEVTPCKSDKIIEGSISITSEDVTRLFNIFLKNHLYYMPLDICL
DCEAIYARCELLFWTILALAGRDFPLISHIQEKVKQLLKDTLFEAVRSVEVVQALLLI
SGWPLPYKTQLDDRSFTCSTLALQYSMRLGLHRPTFAHEFSSRPEVLQFGSFKRMTTW
MGCFLADGREAIRLGLPTFLGADYTLRQSFQDVRIPETLRNLCILAEHTASMNKTLGS
GGENLTGLLEPSPRLELVKQYIGKLDSLFATDQFLRTRNTYIEVVYLTARLQVLSYIL
TEDILSWSVDALEFVNIALHDASRVIRVIAHLPGLEFAPTMVFRYIIYAALVLLRILR
TCEAVALDRKSILSDLTSVYQTLDRLHSGLRNDEHILMTKNFLQIYSNLKDVKVTQPI
KSRGPANIVYDAVRITREAQANHLRQQEKGLLTRQQGNQTEEHNRQENTSFLASLLQN
GGFGAVPNENGETAMLPADPTMVPEIDDQWFNEIFNLVYYM"
gene <129030..>129977
/gene="HIS2"
/locus_tag="AWJ20_3745"
/db_xref="GeneID:30035800"
mRNA <129030..>129977
/gene="HIS2"
/locus_tag="AWJ20_3745"
/product="histidinol-phosphatase"
/transcript_id="XM_018880771.1"
/db_xref="GeneID:30035800"
CDS 129030..129977
/gene="HIS2"
/locus_tag="AWJ20_3745"
/inference="similar to AA sequence:KEGG_Orthology:K04486"
/note="Histidinolphosphatase; catalyzes the eighth step in
histidine biosynthesis; mutations cause histidine
auxotrophy and sensitivity to Cu, Co, and Ni salts;
transcription is regulated by general amino acid control;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0004401 -
histidinol-phosphatase activity [Evidence IEA,IEA];
GO_function: GO:0004401 - histidinol-phosphatase activity
[Evidence IMP] [PMID 14190241]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_process: GO:0008652
- cellular amino acid biosynthetic process [Evidence IEA];
GO_process: GO:0016311 - dephosphorylation [Evidence
IEA,IEA]; GO_process: GO:0000105 - histidine biosynthetic
process [Evidence IEA,IEA,IEA]; GO_process: GO:0000105 -
histidine biosynthetic process [Evidence IMP] [PMID
14190241]"
/codon_start=1
/product="histidinol-phosphatase"
/protein_id="XP_018733428.1"
/db_xref="GeneID:30035800"
/translation="MHSHHSHSSDHCQHASSPLDDMIQRAIDERLLVLCLTEHMPRID
PNHLYPEEIKSNTTVEQLKCVFDTYYKRARQAQIEYKDRIDLLVGFESETISDEYLDY
IDELRKQYKFDMVVGSVHHVSRIPIDYNEETWIRALDATVGTLHSKTDNISDESLQKY
FEEYFDLQYLMLKRLKPSVVGHFDLIKLCAPKGVVPESLQSWPSVWEKITRNVKLAVS
QGGLFEVNSAAVRKGWDTPYPGRDIASLIHELGGEFCLSDDSHQVGHVAQNYKACIRY
LKELGVERVYYLKTKRLADTISIIKDSVSLQELDEWATL"
gene complement(<130088..>131074)
/locus_tag="AWJ20_3746"
/db_xref="GeneID:30035801"
mRNA complement(<130088..>131074)
/locus_tag="AWJ20_3746"
/product="putative ribonuclease H1"
/transcript_id="XM_018880772.1"
/db_xref="GeneID:30035801"
CDS complement(130088..131074)
/locus_tag="AWJ20_3746"
/codon_start=1
/product="putative ribonuclease H1"
/protein_id="XP_018733429.1"
/db_xref="GeneID:30035801"
/translation="MPSQSQKYASCWAGLRNVSGSNIDNRIKWLDRLDSFRLFSQVCE
DDSVSSDDDGTDDGYFSRDEEDYSDNDSFSDDDNLGYEDSGDLFGYSTSRIKIYAVKR
GREKGIFSELQTALDATYRFSHCAMKSFTNFQAACDYMKYRYYAVQKGHVKGIFRLWA
NAVEASAMYPGSRMKSFKSLKLAKLFLALTDKSSVVFTDGTIVDGPDRGPWYGIFYGP
EHPENEFRRQPGKIQTVERAKIKAISRVLTKTLMCGQKYQQIHSSCKSTVDALTSNES
PLPNRDLILNCKVKLKDLAAIGVRLQIIGDQNKDKIDEYREVRELINKAARQ"
gene complement(<132003..>134147)
/locus_tag="AWJ20_3747"
/db_xref="GeneID:30035802"
mRNA complement(<132003..>134147)
/locus_tag="AWJ20_3747"
/product="proline--tRNA ligase"
/transcript_id="XM_018880773.1"
/db_xref="GeneID:30035802"
CDS complement(132003..134147)
/locus_tag="AWJ20_3747"
/inference="similar to AA sequence:KEGG_Orthology:K01881"
/note="Prolyl-tRNA synthetase; N-terminal domain shows
weak homology to prokaryotic posttransfer editing domain,
but does not possess posttransfer editing activity; may
interact with ribosomes, based on co-purification
experiments; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IDA] [PMID 16702403]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0002161 -
aminoacyl-tRNA editing activity [Evidence IEA];
GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
[Evidence IEA,IEA]; GO_function: GO:0016874 - ligase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_function:
GO:0004827 - proline-tRNA ligase activity [Evidence
IEA,IEA]; GO_function: GO:0004827 - proline-tRNA ligase
activity [Evidence IDA] [PMID 16864571]; GO_process:
GO:0006433 - prolyl-tRNA aminoacylation [Evidence IEA];
GO_process: GO:0006433 - prolyl-tRNA aminoacylation
[Evidence IDA] [PMID 16864571]; GO_process: GO:0006450 -
regulation of translational fidelity [Evidence IEA];
GO_process: GO:0006418 - tRNA aminoacylation for protein
translation [Evidence IEA]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="proline--tRNA ligase"
/protein_id="XP_018733430.1"
/db_xref="GeneID:30035802"
/translation="MSSLIKDLEGLSLTHEAPANNEEWASILAKSDVAKFAGPYELTK
VLVFKPKTAKTATPVPVVVVALNSTQTASNIVAKAAGVKEPRLANKDLINEFFGTEPQ
NVSPISITKENASKLKILIDSKAAEKSTLLAIHTDSNAKTAFITGDKLIEAYESIAAD
VTVVDFAKEAAAAAASATPTAGSGADKSAKSGSAGAVAKASANEGKIDGAALIGITSD
KELDFPGWYQQIVTKAELLDYYDVSGCYIMRPGSYSIWEKVQNYLDTRIKSIGVQNAY
FPMFVSSRVLEKEKDHIEGFAPEVAWVTKAGQSDLEEPLAIRPTSETVMYPYYAKWIR
SYRDLPLRLNQWNSVVRWEFKHPQPFLRTREFLWQEGHTAHLTKEAAAEEVLQILEWY
AGVYEELLAVPVIKGTKTDKEKFAGGLYTTTCEGYIPTTGRGIQGATSHALGQNFSKM
FNIVVEDPEGSDKPKINVWQNSWGLSTRVIGVMVMIHSDNKGLVLPPRVASNQVAVIP
VGITAKTTSDERDAIINGAKNVEKTIGDAGVRVFGDYRDNYSPGWKFADAELKGVPLR
VEFGPADLKKNQVVAVRRDNGVKTVIPIASLADEIPKLLETIQADLYTKAKKQFDEHI
VPVTTVAEMTPLLNKKNILLAPFCGEEECEDSIKETTARRDDDEEVDEKAPSMGAKSL
CIPFEQPELKEGTKCVNCDRLAKAHTLFGRSY"
gene <134668..>136368
/gene="DED81"
/locus_tag="AWJ20_3748"
/db_xref="GeneID:30035803"
mRNA <134668..>136368
/gene="DED81"
/locus_tag="AWJ20_3748"
/product="asparagine--tRNA ligase DED81"
/transcript_id="XM_018880774.1"
/db_xref="GeneID:30035803"
CDS 134668..136368
/gene="DED81"
/locus_tag="AWJ20_3748"
/inference="similar to AA sequence:KEGG_Orthology:K01893"
/note="Cytosolic asparaginyl-tRNA synthetase; required for
protein synthesis, catalyzes the specific attachment of
asparagine to its cognate tRNA; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0005829
- cytosol [Evidence IC] [PMID 9605503]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0004812 - aminoacyl-tRNA ligase activity [Evidence
IEA,IEA]; GO_function: GO:0004816 - asparagine-tRNA ligase
activity [Evidence IEA,IEA]; GO_function: GO:0004816 -
asparagine-tRNA ligase activity [Evidence IDA,ISS] [PMID
9605503]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0003676 - nucleic acid
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_process:
GO:0006421 - asparaginyl-tRNA aminoacylation [Evidence
IEA]; GO_process: GO:0006421 - asparaginyl-tRNA
aminoacylation [Evidence IDA,IGI,ISS] [PMID 9605503];
GO_process: GO:0006418 - tRNA aminoacylation for protein
translation [Evidence IEA]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="asparagine--tRNA ligase DED81"
/protein_id="XP_018733431.1"
/db_xref="GeneID:30035803"
/translation="MSESIEKPLESLSVSGAAVYVNEATGIDGATADGSEKSPYQTAA
YALYSSKSENILVAKKLEDGTVSYDKITPSAIKKARKGADGLAKKAEKAAKQAEAQQL
RDAEEAKKIEAAKSISLKQDPTLPKAKVIKLRVAEQHRLQRVQVNGWIHRLRVQKGVA
FIVLRDGTGFLQCVLSGDLANAYQTSTLTLESTVSIYGVIKPVPEGQSAPGGHELIAD
YYDIVGLAPSGDEAFTNKVQESADPSILLDQRHLALRGETLSAVMKVRATLLRAVREV
YEEEGLLEVTPPCMVQTQVEGGSTLFGFQYYGEEAYLTQSSQLYLETCVPALGDVYCI
QESFRAERSHTRRHLSEYTHIEAELGFLSFEDLLDHIERVISRTVAKVLADPVAGPLV
KQLNPNFEPPKTPFMRLEYVKALDWLNEHGIPNEDGEKFKFGDDIAEAAERKMTDIIG
VPILLNRFPLEIKSFYMKKCADDPRVTESVDVLMPGVGEITGGSMRIDNLEELLAGFK
REGINPEPYYWFSDQRKYGSFPHGGYGLGTERILAWLCDRFTVRDCSLYPRFTGRCKP
"
gene complement(<137503..>138333)
/gene="SPS19"
/locus_tag="AWJ20_3749"
/db_xref="GeneID:30035804"
mRNA complement(<137503..>138333)
/gene="SPS19"
/locus_tag="AWJ20_3749"
/product="Sps19p"
/transcript_id="XM_018880775.1"
/db_xref="GeneID:30035804"
CDS complement(137503..138333)
/gene="SPS19"
/locus_tag="AWJ20_3749"
/inference="similar to AA sequence:KEGG_Orthology:K17738"
/note="Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary
enzyme of fatty acid beta-oxidation; homodimeric enzyme
required for growth and sporulation on petroselineate
medium; expression induced during late sporulation and in
the presence of oleate; GO_component: GO:0005782 -
peroxisomal matrix [Evidence IDA] [PMID 9268358];
GO_component: GO:0005777 - peroxisome [Evidence IEA,IEA];
GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
(NADPH) activity [Evidence IEA]; GO_function: GO:0008670 -
2,4-dienoyl-CoA reductase (NADPH) activity [Evidence
IDA,ISS] [PMID 9268358]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_process:
GO:0030437 - ascospore formation [Evidence IEP,IMP] [PMID
7969036]; GO_process: GO:0009062 - fatty acid catabolic
process [Evidence IDA,ISS] [PMID 9268358]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence IEA];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="Sps19p"
/protein_id="XP_018733432.1"
/db_xref="GeneID:30035804"
/translation="MTAPVLFKNMFSLAGKTAVITGGSGGLGLSVSKYLLQAGASLAL
IDNNLPKLEPAAADLSKWYNEPVNKVEYEHKQIISSWACDISDATQVESTIQAIRAHH
AKPLDILINAAGYCENISAVEYPALNIKRIVDVNLNGSLFMAREFAKSLIADNHPGSI
ILVASMSGSIINYPQPQTPYNMTKAGVIHLAKSLASEWAAHNIRVNSLSPGYILTPLT
KAIIDSDARLKAEWESKVPLGRMADPSEFAGPIIYMSSAASSYMTGHDLVVDGGYTFW
"
gene complement(<143207..>144166)
/locus_tag="AWJ20_3750"
/db_xref="GeneID:30035806"
mRNA complement(<143207..>144166)
/locus_tag="AWJ20_3750"
/product="hypothetical protein"
/transcript_id="XM_018880777.1"
/db_xref="GeneID:30035806"
CDS complement(143207..144166)
/locus_tag="AWJ20_3750"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733433.1"
/db_xref="GeneID:30035806"
/translation="MPTVVLPNHTLLSFSDTGRPHTGQYETVVLCHGVGSNIRVFESL
NEVVRSGQVKIRLLAYNQRGYSGSSPLPKGNLKKEEYALEHATDLVEFLEYANEDLKL
PKPPIVVGWSKGTNLLIAIACQSFLPTELRQRGLKYSSAFVLYEPPSSIFALKPDPAF
TKAMTPPEGMSKEEQARTLPQRFEKSFGVYYEYPDPNSIEGAKPATNVSEPAAVRELI
LSAKEPHMIPHGFTWAIGSAKYQRQAVQEALTAKLDIPVLVVWNGMTAPYCKQAALEA
HKMGGRVVQLWNGGNHYAFVGEPRRWLTSILTAVHDSSSSSKL"
gene complement(<144714..>145349)
/gene="PRE5"
/locus_tag="AWJ20_3751"
/db_xref="GeneID:30035807"
mRNA complement(<144714..>145349)
/gene="PRE5"
/locus_tag="AWJ20_3751"
/product="proteasome core particle subunit alpha 6"
/transcript_id="XM_018880778.1"
/db_xref="GeneID:30035807"
CDS complement(144714..145349)
/gene="PRE5"
/locus_tag="AWJ20_3751"
/inference="similar to AA sequence:KEGG_Orthology:K02725"
/note="Alpha 6 subunit of the 20S proteasome; protein
abundance increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IC] [PMID
9087403]; GO_component: GO:0042175 - nuclear outer
membrane-endoplasmic reticulum membrane network [Evidence
IC] [PMID 9087403]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IC] [PMID 9087403]; GO_component: GO:0000502 -
proteasome complex [Evidence IEA]; GO_component:
GO:0005839 - proteasome core complex [Evidence IEA];
GO_component: GO:0019773 - proteasome core complex,
alpha-subunit complex [Evidence IEA]; GO_component:
GO:0019773 - proteasome core complex, alpha-subunit
complex [Evidence IDA] [PMID 9087403]; GO_component:
GO:0034515 - proteasome storage granule [Evidence IDA]
[PMID 18504300]; GO_function: GO:0004175 - endopeptidase
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0003674
- molecular_function [Evidence ND]; GO_function:
GO:0008233 - peptidase activity [Evidence IEA];
GO_function: GO:0004298 - threonine-type endopeptidase
activity [Evidence IEA,IEA]; GO_process: GO:0010499 -
proteasomal ubiquitin-independent protein catabolic
process [Evidence IDA] [PMID 19162040]; GO_process:
GO:0043161 - proteasome-mediated ubiquitin-dependent
protein catabolic process [Evidence IDA] [PMID 11545745];
GO_process: GO:0043161 - proteasome-mediated
ubiquitin-dependent protein catabolic process [Evidence
IDA] [PMID 19029916]; GO_process: GO:0006508 - proteolysis
[Evidence IEA]; GO_process: GO:0051603 - proteolysis
involved in cellular protein catabolic process [Evidence
IEA]; GO_process: GO:0006511 - ubiquitin-dependent protein
catabolic process [Evidence IEA]"
/codon_start=1
/product="proteasome core particle subunit alpha 6"
/protein_id="XP_018733434.1"
/db_xref="GeneID:30035807"
/translation="MTAKLVYNRPLAVSRAVYAIADKAQSNTQQYGRRPYGVGLLIAG
FDETGPHLYEFLPSGSVLEYVGTAIGARSQAARTYLERNYEKFGDSSLEELVVHGLNA
LRDTLAQDKELTSKNTSIAVVGLDTPFKLLDDDEVVPWLEKLDSVSRSSHPVQTEEQP
ATETQPEPAAATTSTDAPADADAPADAAGAAGSDSAAEPRPDDDSNMDTTQ"
gene complement(<147370..>148341)
/gene="ERV29"
/locus_tag="AWJ20_3752"
/db_xref="GeneID:30035808"
mRNA complement(<147370..>148341)
/gene="ERV29"
/locus_tag="AWJ20_3752"
/product="Erv29p"
/transcript_id="XM_018880779.1"
/db_xref="GeneID:30035808"
CDS complement(147370..148341)
/gene="ERV29"
/locus_tag="AWJ20_3752"
/note="Protein localized to COPII-coated vesicles;
involved in vesicle formation and incorporation of
specific secretory cargo; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0030134 - ER to Golgi transport vesicle [Evidence IDA]
[PMID 11157978]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0006888 - ER to Golgi vesicle-mediated
transport [Evidence IPI] [PMID 11157978]"
/codon_start=1
/product="Erv29p"
/protein_id="XP_018733435.1"
/db_xref="GeneID:30035808"
/translation="MSNRYQMPLDGGDVKTRRQASGGISGYDSAPMGGANFSSYPGAA
ASSPSFARRAHEVSEKIDNFLGTVGTPVKPYLPGIGRFLIIATFVEDAFRIVTQWSDQ
VGYIWNVRGLPHIIAVLFLSINVVAMLIGSFSVVFKKRLEIGVGALLFVVVSQALVYG
LIFKFSFFFRNVSLVGGLLIVLSDAFVRDRRSLSLPGLPVLEDKDRSKYLQLAGRILL
IFLFIAYMVTRNWTVIGGFFNVIGLVACIMVVVGFKARLSASLLVVLLSVQNLLTNPY
WRYASRNPARDLLRYEYFQTLSIVGGLILLVSSGAGRISIDEKKKIY"
gene <149580..>152039
/locus_tag="AWJ20_3753"
/db_xref="GeneID:30035809"
mRNA <149580..>152039
/locus_tag="AWJ20_3753"
/product="hypothetical protein"
/transcript_id="XM_018880780.1"
/db_xref="GeneID:30035809"
CDS 149580..152039
/locus_tag="AWJ20_3753"
/inference="similar to AA sequence:KEGG_Orthology:K15631"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733436.1"
/db_xref="GeneID:30035809"
/translation="MPEAAEVLDGYGYDGKIDEIRKLYYGQLDKNGNDSTVYLDHAGT
TLYPTSIVDKFSATLVKNIYGNPHSKSPSSQNTERWIERARGQFLGLFGADVRQYSVV
FTANSTAAVKLIGEGLSTLPNSKYTYLKDSHTSLVGLRNLASSFEVTGDIEQYLLSLE
RQLEKENSTHSNSDLEVDDFNGNGHKSTSGNSELLSWPAQSNFSGKRYPCEEWLNRVR
FINRNNSSSDHRLYTLLDAAAISSSKPIDLLKLNPDFVIISCYKIFGFPDLGALIVKN
EVLPQFFANRKYFGGGTLDSLASETDFVARKSLTGSQALEDGTLPFHSIIGLSIAIDE
YKRIYGSFDNISLHTSSLAGYLHRQLERLKYSNGQPLVHIYGPNTEEYKIGEHQGPIV
AFNLKFPSGEWIGYSDLETIASSQNINLRTGTLCNSGSASTWMNISDSEIISNYSSGH
VCGDTNDIMNGKPTGAVRVSLGACSSIGDVDALINCLTDFYLKPFNQELAKAPLTSAK
SLTTTARITALSIYPIKSCAAYNISTPWVVGANGLKWDREFCLVNMSSYSVLSLKKYP
KMVHILPKIDGDRLLIRYNDNEISVPLRLLPSNSNSETTLKVCGNNIFAQIYTDATVV
KFFTSALGIDCTLARIHNDPQPATNTEITREQPGTMVNSSPILLISQSSVNKLSSNTL
NSSSSDDDQRSRHQPVDRQIFRANITISGPTPYSEDEWTAIQTDTVNFKVCIKSGIPT
PDSSEARGATGSGAEPQPPEATTTRPILTGQLLGKCQRCNMVCVDASTGQRNQAPFKA
LHRTRKENVSYSRKKGRKRHC"
gene <153893..>154890
/gene="UBI4"
/locus_tag="AWJ20_3754"
/db_xref="GeneID:30035810"
mRNA join(<153893..154414,154870..>154890)
/gene="UBI4"
/locus_tag="AWJ20_3754"
/product="ubiquitin"
/transcript_id="XM_018880781.1"
/db_xref="GeneID:30035810"
CDS join(153893..154414,154870..154890)
/gene="UBI4"
/locus_tag="AWJ20_3754"
/inference="similar to AA sequence:KEGG_Orthology:K08770"
/note="Ubiquitin; becomes conjugated to proteins, marking
them for selective degradation via the ubiquitin-26S
proteasome system; essential for the cellular stress
response; encoded as a polyubiquitin precursor comprised
of 5 head-to-tail repeats; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IC] [PMID 8982460];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0043008 - ATP-dependent protein binding
[Evidence TAS] [PMID 8982460]; GO_function: GO:0031386 -
protein tag [Evidence ISS] [PMID 3038523]; GO_process:
GO:0006281 - DNA repair [Evidence IEA]; GO_process:
GO:0030437 - ascospore formation [Evidence TAS] [PMID
8982460]; GO_process: GO:0006974 - cellular response to
DNA damage stimulus [Evidence IEA]; GO_process: GO:0016579
- protein deubiquitination [Evidence TAS] [PMID 8982460];
GO_process: GO:0006513 - protein monoubiquitination
[Evidence TAS] [PMID 8982460]; GO_process: GO:0000209 -
protein polyubiquitination [Evidence TAS] [PMID 8982460];
GO_process: GO:0016567 - protein ubiquitination [Evidence
IMP] [PMID 8035826]"
/codon_start=1
/product="ubiquitin"
/protein_id="XP_018733437.1"
/db_xref="GeneID:30035810"
/translation="MQIFVKTLTGKTITLEVESSDTIDNVKTKIQDKEGIPPDQQRLI
FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV
KTKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL
TGKTITLEVESSDTYRQRQD"
gene <159786..>161393
/gene="INO1"
/locus_tag="AWJ20_3755"
/db_xref="GeneID:30035811"
mRNA <159786..>161393
/gene="INO1"
/locus_tag="AWJ20_3755"
/product="inositol-3-phosphate synthase INO1"
/transcript_id="XM_018880782.1"
/db_xref="GeneID:30035811"
CDS 159786..161393
/gene="INO1"
/locus_tag="AWJ20_3755"
/inference="similar to AA sequence:KEGG_Orthology:K01858"
/note="Inositol-3-phosphate synthase; involved in
synthesis of inositol phosphates and inositol-containing
phospholipids; transcription is coregulated with other
phospholipid biosynthetic genes by Ino2p and Ino4p, which
bind the UASINO DNA element; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_function:
GO:0004512 - inositol-3-phosphate synthase activity
[Evidence IEA,IEA]; GO_function: GO:0004512 -
inositol-3-phosphate synthase activity [Evidence IMP]
[PMID 7016881]; GO_function: GO:0016853 - isomerase
activity [Evidence IEA]; GO_process: GO:0006021 - inositol
biosynthetic process [Evidence IEA,IEA,IEA]; GO_process:
GO:0006021 - inositol biosynthetic process [Evidence IMP]
[PMID 7016881]; GO_process: GO:0006629 - lipid metabolic
process [Evidence IEA]; GO_process: GO:0008654 -
phospholipid biosynthetic process [Evidence IEA,IEA]"
/codon_start=1
/product="inositol-3-phosphate synthase INO1"
/protein_id="XP_018733438.1"
/db_xref="GeneID:30035811"
/translation="MAPSVNTHSAATAASQAPGYGKSGFTTVEVQTDKVRVADDAIYS
TYAYENAVVEKVDGKYKVKPTVQEYEFKLDTKVPKTGLLLVGLGGNNGSTLTAAVTAN
RHNISFNTKNGKIEPNYFGSVTQAATIKLGIDANGKDVYAPFNEIVPLLHPNDFVIGG
WDISKLNLAGAMQRAQVLDYDLQRQLVKHLEQIVPLPSIYYPDFIAANQSERADNLIP
GEDKWAHVEHIRKDIREFKEKNDLDKVIVLWTANTERYADIIEGVNDTADNLLKAVKE
SHSEVSPSTIFAIASILEGVPYINGSPQNTFVPGCIELAEKHNAFIGGDDFKSGQTKL
KSVLAQYLVDAGIRPVSVASYNHLGNNDGYNLSAPKQFRSKEISKASVIDDMIESNQI
LYNDKYGKTVDHCIVIKYVPAVGDSKVAMDEYHSELMLGGHNTVSIHNVCEDSLLATP
LIIDLVVVSEFLSRVTYKKKNSEEDYHQFHSVLSFLSYWLKAPLTRPGFDPVNGLNKQ
RAGLENLIRGLVGLAPHNDLKLEERTV"
gene complement(<160002..>160460)
/locus_tag="AWJ20_3756"
/db_xref="GeneID:30035812"
mRNA complement(<160002..>160460)
/locus_tag="AWJ20_3756"
/product="myo-inositol-1-phosphate synthase"
/transcript_id="XM_018880783.1"
/db_xref="GeneID:30035812"
CDS complement(160002..160460)
/locus_tag="AWJ20_3756"
/codon_start=1
/product="myo-inositol-1-phosphate synthase"
/protein_id="XP_018733439.1"
/db_xref="GeneID:30035812"
/translation="MGPLVFTRNQVISTLRLVSGDEIWVVDRRQGNNLLQVLDQLTLK
VIVKNLSTLHSTSQIELRDIPTANDEVIRVQKRDNLVERSINILSISINTKLDGSSLS
NRTEIVGLNLTILGIERDVVTVGSDSSSQGRTVITTKTNEKQTGLGDLGV"
gene complement(<162215..>164302)
/gene="KIP2"
/locus_tag="AWJ20_3757"
/db_xref="GeneID:30035813"
mRNA complement(<162215..>164302)
/gene="KIP2"
/locus_tag="AWJ20_3757"
/product="Kip2p"
/transcript_id="XM_018880784.1"
/db_xref="GeneID:30035813"
CDS complement(162215..164302)
/gene="KIP2"
/locus_tag="AWJ20_3757"
/note="Kinesin-related motor protein involved in mitotic
spindle positioning; stabilizes microtubules by targeting
Bik1p to the plus end; Kip2p levels are controlled during
the cell cycle; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005881 - cytoplasmic
microtubule [Evidence IDA] [PMID 9693366]; GO_component:
GO:0005856 - cytoskeleton [Evidence IEA,IEA];
GO_component: GO:0005871 - kinesin complex [Evidence IEA];
GO_component: GO:0005871 - kinesin complex [Evidence TAS]
[PMID 9153752]; GO_component: GO:0005874 - microtubule
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0008017 - microtubule binding [Evidence IEA];
GO_function: GO:0003777 - microtubule motor activity
[Evidence IEA]; GO_function: GO:0003777 - microtubule
motor activity [Evidence TAS] [PMID 9153752]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0051301 - cell division [Evidence IEA];
GO_process: GO:0008152 - metabolic process [Evidence IEA];
GO_process: GO:0007018 - microtubule-based movement
[Evidence IEA]; GO_process: GO:0007067 - mitotic nuclear
division [Evidence IEA]; GO_process: GO:0007026 - negative
regulation of microtubule depolymerization [Evidence IMP]
[PMID 9693366]; GO_process: GO:0030473 - nuclear migration
along microtubule [Evidence IMP] [PMID 9693366]"
/codon_start=1
/product="Kip2p"
/protein_id="XP_018733440.1"
/db_xref="GeneID:30035813"
/translation="MSMSISSSPNKRPPVRPPSVLSLNGMGMPSPSHSRPQTPRLSRR
PASSLSLRSPTPSLRRPATPSQYGPMSGSGSAVSPSMSYTGNIKVSVRVKPNVISQQP
ESHTAAAVAASSWIVDPANSCISTKEVGDFNFDHVFDGAGLPNSHIYQNSVEPLVEQV
MQGFHGTVFAYGMTGSGKTFSMQGTRNDPGIIPLAAESIFNHIDNDPSRSYKVTLGYL
EIYNEHLNDLLTPGDKTNPGEETIKLRDDVLRGVRAMGLKEVDVRSSQELLDYIAQGD
SIRRTEGTDFNSRSSRSHAVVQISIESIPNSDSTGVRSHQSQHQQLQRLQSTLYLCDL
AGSERAVSQSERRKEGAFINKSLLTLGTVIARLSSNNNAGHIPYRDSKLTRLLQPALS
GKSLVSVLCTIQTTAYSYIETLSTLRFAARAKNIVVSAKRNDDADPSAKTVERLLQQV
AAQKLEIEQLRANGSSGSSSSATSTNGSGLGIGYASSSGSSSGLPSPTLPSMAHMSQL
EAENRILHERVEHLSRLCDDSRLEDILGVTADESDLDGDSNKHLQQIEEYKSYIAHLE
KQLYNQEINRSINGRETPSESGQSHLSSGSHYNDIIRDLKEEIEELRESNRDKDRIIN
ALRASNKRKENLAASLSSTAANSLAYSRYYGSNTVSPPPIAEDRADTAKENIPSDIVP
TSKLSEISLETNR"
gene <165741..>166356
/gene="TPM2"
/locus_tag="AWJ20_3758"
/db_xref="GeneID:30035814"
mRNA join(<165741..165758,165913..>166356)
/gene="TPM2"
/locus_tag="AWJ20_3758"
/product="tropomyosin TPM2"
/transcript_id="XM_018880785.1"
/db_xref="GeneID:30035814"
CDS join(165741..165758,165913..166356)
/gene="TPM2"
/locus_tag="AWJ20_3758"
/inference="similar to AA sequence:KEGG_Orthology:K17945"
/note="Minor isoform of tropomyosin; binds to and
stabilizes actin cables and filaments, which direct
polarized cell growth and the distribution of several
organelles; appears to have distinct and also overlapping
functions with Tpm1p; TPM2 has a paralog, TPM1, that arose
from the whole genome duplication; GO_component:
GO:0032432 - actin filament bundle [Evidence IDA] [PMID
9864365]; GO_component: GO:0000142 - cellular bud neck
contractile ring [Evidence IDA] [PMID 9864365];
GO_component: GO:0005737 - cytoplasm [Evidence IEA];
GO_component: GO:0005856 - cytoskeleton [Evidence
IEA,IEA]; GO_function: GO:0003786 - actin lateral binding
[Evidence TAS] [PMID 9153752]; GO_process: GO:0051017 -
actin filament bundle assembly [Evidence IGI] [PMID
9864365]; GO_process: GO:0007015 - actin filament
organization [Evidence TAS] [PMID 10652251]; GO_process:
GO:0008154 - actin polymerization or depolymerization
[Evidence TAS] [PMID 10652251]; GO_process: GO:0007118 -
budding cell apical bud growth [Evidence TAS] [PMID
10652251]; GO_process: GO:0007119 - budding cell isotropic
bud growth [Evidence TAS] [PMID 10652251]; GO_process:
GO:0030010 - establishment of cell polarity [Evidence TAS]
[PMID 10652251]; GO_process: GO:0006887 - exocytosis
[Evidence TAS] [PMID 10652251]; GO_process: GO:0008298 -
intracellular mRNA localization [Evidence TAS] [PMID
10652251]; GO_process: GO:0000001 - mitochondrion
inheritance [Evidence TAS] [PMID 10652251]; GO_process:
GO:0000011 - vacuole inheritance [Evidence TAS] [PMID
10652251]; GO_process: GO:0016192 - vesicle-mediated
transport [Evidence TAS] [PMID 10652251]"
/codon_start=1
/product="tropomyosin TPM2"
/protein_id="XP_018733441.1"
/db_xref="GeneID:30035814"
/translation="MDKLRETEAAQEKADEANDKVKTLEQENLQKDQEIASLTHKNSL
LEQEVEELESKLNDAKGAAEEGAAHGTQNESLTKKISLLEEELENSDKNLRETTEKLR
QTDVKAEHFERKVTSLEQERDDWEKKFEELSEKYSAAKAELDEITQQLESI"
gene <166994..>168097
/locus_tag="AWJ20_3759"
/db_xref="GeneID:30035815"
mRNA <166994..>168097
/locus_tag="AWJ20_3759"
/product="hypothetical protein"
/transcript_id="XM_018880786.1"
/db_xref="GeneID:30035815"
CDS 166994..168097
/locus_tag="AWJ20_3759"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733442.1"
/db_xref="GeneID:30035815"
/translation="MEVCTRSGVYRGVGCNNARTGFGFRRFLSSANWSPCNDGNIATI
LHVRKLSTLLKKKNRTDSEFTQLTKHSVSRNTLHHSSNIRNGLDQGRRTIDPVGTIRH
ARTYATYRGPGRGGLISSVWNLVPAPVRAFTVVAGTAAGFFFISVPLLIICGPPLALG
LWLYTRRMKRIANELYHQRWNNLGSYHLTFEDQLDQTAKSQNPADDLFTILMNKAGGA
TSDAERIARDRILDAVESDEQNLAGLLNIKNQRDIDNLGLTGLEGIQQDFRGSSQGFQ
EKMEIRTLGLVDKAQSPPRRLANVTLVIQSDGYRNKKMRVELEVLAGYLRSSERIILD
ANPVGDKSSGSQSSSYAQDTIIEVDPSHYRRTK"
gene <173456..>179983
/gene="ACC1"
/locus_tag="AWJ20_3760"
/db_xref="GeneID:30035817"
mRNA <173456..>179983
/gene="ACC1"
/locus_tag="AWJ20_3760"
/product="acetyl-CoA carboxylase ACC1"
/transcript_id="XM_018880788.1"
/db_xref="GeneID:30035817"
CDS 173456..179983
/gene="ACC1"
/locus_tag="AWJ20_3760"
/inference="similar to AA sequence:KEGG_Orthology:K11262"
/note="Acetyl-CoA carboxylase, biotin containing enzyme;
catalyzes carboxylation of cytosolic acetyl-CoA to form
malonyl-CoA and regulates histone acetylation by
regulating the availablity of acetyl-CoA; required for de
novo biosynthesis of long-chain fatty acids; ACC1 has a
paralog, HFA1, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IDA] [PMID 9438137]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0003989 - acetyl-CoA
carboxylase activity [Evidence IEA,IEA]; GO_function:
GO:0003989 - acetyl-CoA carboxylase activity [Evidence
IMP,ISS] [PMID 8098706]; GO_function: GO:0004075 - biotin
carboxylase activity [Evidence IEA,IEA]; GO_function:
GO:0004075 - biotin carboxylase activity [Evidence ISS]
[PMID 1350093]; GO_function: GO:0004075 - biotin
carboxylase activity [Evidence IMP] [PMID 6108218];
GO_function: GO:0003824 - catalytic activity [Evidence
IEA,IEA]; GO_function: GO:0016874 - ligase activity
[Evidence IEA,IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA,IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006633
- fatty acid biosynthetic process [Evidence IEA,IEA];
GO_process: GO:0006631 - fatty acid metabolic process
[Evidence IEA]; GO_process: GO:0006629 - lipid metabolic
process [Evidence IEA]; GO_process: GO:0042759 -
long-chain fatty acid biosynthetic process [Evidence IMP]
[PMID 6103540]; GO_process: GO:2001295 - malonyl-CoA
biosynthetic process [Evidence IEA]; GO_process:
GO:0008152 - metabolic process [Evidence IEA,IEA];
GO_process: GO:0006998 - nuclear envelope organization
[Evidence TAS] [PMID 9442897]; GO_process: GO:0006606 -
protein import into nucleus [Evidence IMP] [PMID
12730220]"
/codon_start=1
/product="acetyl-CoA carboxylase ACC1"
/protein_id="XP_018733443.1"
/db_xref="GeneID:30035817"
/translation="MATPEDLEANAEYIRMADQYVEVPGGTNNNNYANVELIVDIAER
TNVQAVWAGWGHASENPHLPESLAASPKKIVFIGPPGSAMRSLGDKISSTIVAQHAKV
PCIPWSGTGVDQVSVDKESGLVSVPDDVYIKGCCTTPEDGLEKAKKIGFPVMIKASEG
GGGKGIRKVEHEDKFISLYKQVEAEIPGSPIFIMQLAGNARHLEVQLLADQYGNNISL
FGRDCSVQRRHQKIIEEAPVTIARPDTFTAMEKAAVRLGQLVGYVSAGTVEYLYSHAD
DKFYFLELNPRLQVEHPTTEMVTGVNLPAAQLQIAMGIPLHNIRDIRLFYGVDPKTQT
PIDFDFSTELSKKSQRRPVPKGHTTACRITSEDPGEGFKPSSGMMHELNFRSSSNVWG
YFSVGNQGGIHSFSDSQFGHIFAFGENRQASRQHMVVALKELSIRGDFRTTVEYLIKL
LETPDFEDNTITTGWLDELISKKLTAERPDPYLAVICGAVTKAHVQCEGIINDYKTSL
EKGQAPSKETLKSLFAIDFIYEGERYKFTATRSSQDSYTLFINGSRVDVGVRSLSDGG
LLVSIGGKSHSVYWKEEVGATRVSVDAKTCLLEVENDPTQLRTPSPGKLVRFLVESGE
RVKAGQPYAEVEVMKMYMPLVAAEDGIVQLIKQPGSTLEAGDILGILALDDPSRVKHA
KPFEGQLPEYGPSSLIGNKPSQRFYVLHHILENILSGFDNQVVMNSTLKELIEVLRNP
ELPYGEWSQQASALHSRLPAKLDANLSGIVDKAKQRKAEFPAKQLLKAIEKAAPEKAS
EAGIYQSVVEPLTKICVRYSEGLVAHEYHVIANLLQEYIDVESLFSGSHSREEDVILR
LREENKDNLRKVVMKVWSHSRVGAKNNLILAILSEYQPSKVDSSLIAAHIRPVLKNLV
ELESRATAKVVLKAREILIQCALPSLKERSDQLEHILRSSVVESSYGEVGLERRTPSY
EVLREVVDSRYTVFDVLIQFFTHPDPYIVLAALELYVRRASRAYVIPELTYHMDSSSP
AVCYKFRLPNLNNAAYNALASASAGSSNVSSPDGASSPVRTHFSRAISISDLSFLIGK
NDTSPLRTGVVAPAASLDDIDEALSRALELFPRSNGGLEIKVNGGSLAQQRGVPSAAG
SNNGSNGQKNPLTNICSVVVSDSDPDASDDDILDRIADVLEDNKDDLVACSVRRLTFL
IGGDVGVYPKYFTFRGPDYQEDKTIRHIEPALAFQLELGRLSNFEIKPVFTDNRNIHI
YEAVAKNSSSDKRFFTRGIVTPGRIRDDISTPSYLISEANRLMTDILDALEIVETSNT
DLNHIFINFTAVFTLDPKQVEDAFGGFLERFGRRLWRLRVTGAEIRIMISDPTSGATF
PLRAMINNVSGYVVKTDMYAEVKNNKGDWVYKSLGATPGSMHSRPVNTPYPTKEWLQP
KRYKAHLMGTTYCYDFPELFRQAIISQWKKVPNAKVPEDVFSYSELIIDETEQLSEIT
REPGANNVGMVAWKCFAKTVEYPAGRKFVVIANDITYKIGSFGPQEDNFFNKVTELAR
AEGIPRIYLSANSGARIGVAEEIIPLFSVAWNDAENPSKGFRYLYLTPEHYEQLNKSA
KKTVSCEHIVEDGEVRYKIEFIIGAEDGLGVECLKGSGLIAGATSRAYKDIFTITLVT
CRSVGIGAYLVRLGQRAIQIEGQPIILTGAPAINKLLGREVYSSNLQLGGTQIMYRNG
VSHLTANDDLAGVEKIVNWMSYVPAQRNLPVPILSLGDNWDRDVEFVPSKSEPYDVRW
LIAGRENGDHFEAGLFDKGSFQETLSGWAKGVVVGRARLGGIPMGVIGVETRAVENMI
PADPANPDSTEVMVQEAGQVWYPNSAFKTAQAINDFNHGEQLPLMILANWRGFSGGQR
DMYNEVLKYGSYIVDALVDFKQPIMTYIPPTGELRGGSWVVVDPTINSDMMEMYADAE
ARAGVLEPEGMVGIKFRRDKLLSTMKRLDPKYAELCREAERKDITPAETSSIKVKLVE
REKLLLPIYQQISVQFADLHDRAGRMEAKNTIRKVLDWQNARRFFFWRVRRRVNEEYL
ISRIAKALPKSTRLEQLARLKSLYTGHVENPESDREVAEWLEANAQKLTAAISQLAAD
SSVQQIISTLRSATGETAVRAVTEAFKTLSASDREAVLKSLQS"
gene complement(<177241..>177642)
/locus_tag="AWJ20_3761"
/db_xref="GeneID:30035818"
mRNA complement(<177241..>177642)
/locus_tag="AWJ20_3761"
/product="hypothetical protein"
/transcript_id="XM_018880789.1"
/db_xref="GeneID:30035818"
CDS complement(177241..177642)
/locus_tag="AWJ20_3761"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733444.1"
/db_xref="GeneID:30035818"
/translation="MSVLTTYPDTLLIMARRGKVAPEVGSEIMIRISAPVTRRRHRRR
PNLSKNPPKASSTCLGSKVKTAVKLMKMWLRSVLEVSTISRASRMSVMRRLASEIRYD
GVEISSLIRPGVTIPLVKNLLSEDEFLATAS"
gene complement(<180252..>182000)
/gene="ERT1"
/locus_tag="AWJ20_3762"
/db_xref="GeneID:30035819"
mRNA complement(<180252..>182000)
/gene="ERT1"
/locus_tag="AWJ20_3762"
/product="Ert1p"
/transcript_id="XM_018880790.1"
/db_xref="GeneID:30035819"
CDS complement(180252..182000)
/gene="ERT1"
/locus_tag="AWJ20_3762"
/note="Transcriptional regulator of nonfermentable carbon
utilization; GFP-fusion protein localizes to cytoplasm,
nucleus; null mutation affects periodicity of
transcriptional and metabolic oscillation; plays role in
restricting Ty1 transposition; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003677 - DNA binding [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0043565 - sequence-specific DNA
binding [Evidence IDA] [PMID 19111667]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 19158363]; GO_function: GO:0000981 -
sequence-specific DNA binding RNA polymerase II
transcription factor activity [Evidence IEA]; GO_function:
GO:0004871 - signal transducer activity [Evidence IEA];
GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
GO_process: GO:0008150 - biological_process [Evidence ND];
GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
GO_process: GO:0006357 - regulation of transcription from
RNA polymerase II promoter [Evidence IEA]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA,IEA]; GO_process: GO:0007165 - signal
transduction [Evidence IEA]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Ert1p"
/protein_id="XP_018733445.1"
/db_xref="GeneID:30035819"
/translation="MNNTSLQRDTRAVVSLGLGNNGQGPKNDTGLRPQLQSQPLVEDW
DTSSSNSMTPILDSSVGMMSSDNSNGNTDRKEVQKNSTHNNSSGGVRKERKEKRPKTS
RACIHCQTAHVTCDSGRPCKRCIKKGLESSCRDGNRKRAKYLAGEDPTLIPESNVGIP
NIANASLSPLNPLSSSDNRLLPSIPDNLLTGNPGNMGPMAGVTGQLGNGLTGTIPLSA
NGQNNGSPPIGPVGSSQNQNSFTPIPQSLEDPFLHNDMSQYSFGSRATNLEYSILSNI
LRTNIESQHPQLGNQGNQTTTSTPESDISLQGTYFPELKISDFEALDSGSNPLLLPGS
QQQQQQQPHQRQSLQKQQQKPQQTQQQELPLPQQKTELNQSNSVLQTQLSNDVIPHRF
NSPEEVYQYVRSPFPYTQGFHELIAYLRSRFDKRHLMHMARAMAAYRPSFIATTKLLK
EQDLIFMEQCFQRTLMEFEKIIASSGTPTIIWRRTGQIAAVGKEFCILTGWSPERLVS
NTTFIVELMDDKSVTDYFDVFSRLAFGDSRGVSMTECTLLKPNGEKVPTACTWTVKRD
VFDIPMMIIGNFLPIL"
gene <185275..>187482
/gene="AAP1"
/locus_tag="AWJ20_3763"
/db_xref="GeneID:30035820"
mRNA <185275..>187482
/gene="AAP1"
/locus_tag="AWJ20_3763"
/product="Aap1p"
/transcript_id="XM_018880791.1"
/db_xref="GeneID:30035820"
CDS 185275..187482
/gene="AAP1"
/locus_tag="AWJ20_3763"
/inference="similar to AA sequence:KEGG_Orthology:K13721"
/note="Arginine/alanine amino peptidase; overproduction
stimulates glycogen accumulation; AAP1 has a paralog,
APE2, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0004177 -
aminopeptidase activity [Evidence IEA]; GO_function:
GO:0004177 - aminopeptidase activity [Evidence ISS] [PMID
8100228]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0008237 -
metallopeptidase activity [Evidence IEA,IEA]; GO_function:
GO:0008233 - peptidase activity [Evidence IEA];
GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
GO_process: GO:0005977 - glycogen metabolic process
[Evidence IMP] [PMID 8100228]; GO_process: GO:0006508 -
proteolysis [Evidence IEA,IEA]; GO_process: GO:0006508 -
proteolysis [Evidence IMP] [PMID 8100228]"
/codon_start=1
/product="Aap1p"
/protein_id="XP_018733446.1"
/db_xref="GeneID:30035820"
/translation="MEATDCRRAFPSFDEPALKATFDITLVADKELTALSNMDVKEEK
ILESGKKATSFNTSPVMSTYLVAFIVGELSYVESNYFRVPVRVYATPGMEDRCKFSAE
LGARTLEFFEKKFDVPYPLPKMDMVGIHDFSAGAMENWGLVTYRVVDLLFDEENDNAN
TKQRVAEVVQHELAHQWFGNLVTMDWWDGLWLNEGFATWMSWYSCNSFYPDWKVWETY
VGDNLQSCLALDSLRSSHPVEVPVKRADEVNQIFDAISYSKGSCIVKMVSIILGEDTF
IRGISRYLKRHQYGNTETGDLWKALSEESGLDVAGIMDTWTSKVGFPVLTVSEDGNTI
HVRQNRFLATGDVKPEEDTTIYPLSLALRNKSGKVENINLDKREISIDLSKYDASSAF
YKLNADQAGLYRTLYPPERVVKLSQAGADNLLSVEDRVGLIADTASLSVSGYQKTSAL
LDLISLWKNEKEPNVWMEMLKRFGAIRKTWIFQPESTTDALKQLLGELVLPKVNELGW
NFHAEESLLQQQLKGTLFSSAVSSENDKIVKEALSLFEKYASGDAKAVHPNIRAAVFG
AAAKYGDEATWQRLVDVYTSPKSSVEGLAAITALGGAKNVDLQKKTIKLTLDGTVRSQ
DVFYVLNGLSTNVAGIQILWDWLREEWDAITKKFPANMGLLLNIIYICTGSLATPQHL
KEFDEFFKSKDTKGFDKAVSISRDRLVSRIAWLERDVKDVEEWLAKHQSSTKL"
gene <194525..>195049
/locus_tag="AWJ20_3764"
/db_xref="GeneID:30035821"
mRNA <194525..>195049
/locus_tag="AWJ20_3764"
/product="hypothetical protein"
/transcript_id="XM_018880792.1"
/db_xref="GeneID:30035821"
CDS 194525..195049
/locus_tag="AWJ20_3764"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733447.1"
/db_xref="GeneID:30035821"
/translation="MSHSGWADSVIPTESVANTDSPWAEIESENKSENKSEDAPGREV
VSSATGPSGRSNTPVIRINSSSSTSSSQPDNALTVEGDDDIEVTDAIEGQNEHPFSLS
PDLNRSPGQIGVVSSCESSPDAVHTSGDDAHILQKENDDLRTATSESDNIIMELNMDT
TVRYLSECWDDIVG"
gene <195959..>201064
/gene="RIM15"
/locus_tag="AWJ20_3765"
/db_xref="GeneID:30035822"
mRNA <195959..>201064
/gene="RIM15"
/locus_tag="AWJ20_3765"
/product="protein kinase RIM15"
/transcript_id="XM_018880793.1"
/db_xref="GeneID:30035822"
CDS 195959..201064
/gene="RIM15"
/locus_tag="AWJ20_3765"
/inference="similar to AA sequence:KEGG_Orthology:K12767"
/codon_start=1
/product="protein kinase RIM15"
/protein_id="XP_018733448.1"
/db_xref="GeneID:30035822"
/translation="MEDSDAVPAPPLVLCRICERHYTTWWFERHSELCMIEHRAASEL
EETHEAVLEHRLVITGLLGALELRMNFHVFDSPLTPSGSTSSYGSVTGRSGSSSGSGS
GSIGLGPSAGGGSGSGGVPSLEYRGYTLQLPLFPSDLVSNITSSGPGVTNNAVNIPNT
GGSVGSIGSLPSSLGSPGPVTVGSAGSIPINGSPPRSPRLASSPLSSGSNRRALQRPG
AIPISASSVPHPFMGKRSPIRIMELLLDLCDAAMQIKNPEIIIDEHNEEGEIRVHAHR
SEIKIYEVLNWMPPSSIEDPGLALLCDDTMKFAREKAAAALRMGNTLLYSEKIRRETE
LQVQQVIQDIGEKVATERDYDDSMLVDYNSDSRNSQVFFDSDRSSSGRGFRENRLGGV
GRKGSNGGASTGSNAGSGIFSNAYLQADVLPNSGNATPPRVPRESNISPYQLPAQPTP
TSMLQMQRLNLEDSDSGEHRELSPSPSSFSPSAPAAAPAQPPAPAPSQIPLATPSAAS
SPLSQIAHLATAAPASPAAFTTAPAVQGQPSHIASPSMAAPRPIAPLSGNTLTPKLLL
NINTSMEHEAISPTRRLSTSDGGYAGYGSAFGSGITPTNVANISSPGGVLLRDRFVTR
SRGSGGSSIGSHGSMDINSEDLPTNIGELDLNSPVHSGGTSSGSSSNTASAGPMKLSA
RKSMTNMSSARSFGSPITNLQRTRTSGGPAPGPIPAVASTVLASNSPSTPGTSPLLFP
RDFDRHHHRQSSAASDFTRAPVSPLLTSTVPPMKVSQPSIRDYEIISPISKGAFGSVY
LSKKKVTGEYFAIKVLKKADMIAKNQVMNVKNERAIMMSQADSPYVAKLFATFQSKEY
LFLVMEYLNGGDCAALVKALNGVPADWCQQYIAEIVVITENLHQRGIVHRDLKPDNLL
IDNRGHLKLSDFGLSRMGAVGRHTRMKDGQTSAPRGGSGSMGTGTATASATTSTGGTV
IAAGSGTSTGSGNGGTSTPSGLESPADINALLARSSVFGSINNNATNAFGITPGITGA
HENISLVPGYFNYARSASSSYFYDHKSNVRTDSSDSERARDHNSPTPDPSGKNSTNSS
MAVDETSTTTNVSQVVKSSSRTSSSSGSTPRVVLYDPDDTSSRRFVGTPDYLAPETIQ
GNGQDEMSDWWSVGCIIFEFWYGYPPFHADSPEKVFRNILARNIQWPSELLEDKPGTD
LANDLISKLLAVDQKQRLGANGVDEIRQHPFFSKDLDWDHLYDDDQAVFVPAVDNPES
TDYFDSRGAVMTEFPDDSAVAGDDDNSMAIESDSSDNNSSKQTGTSPLSTKKEPRMKM
LPLHIPPHVHDSTRIRRLSEPSLADDFGSFSFKNLPVLEKANKEVISKIKSETESGRQ
RGLSVSAKGSPTSSKVITPAWCSPGQPSSSSPGTFPNTSTSGTHGSSMSSSISNIATH
TGSVASSTSDDIFADSESPSRRLQQDLHLSISSIGSKGGDLDSESGSPRHKRHSSSYR
RLSNMESSPELGEQYRKQSLASASRNYQVFDMSPSSSDNEDSRGAALLRVQKRRQLSR
RMSSFALNVEPSPRALDILVCDGNPVWRYTTEKMLVSLGCRVVTVATGEEAIRRSVGD
VIFDIIFVEYKIGKWSGVDITRLIHSTLNPNKSTPVVVMTNLIKEAEETVKQAQEENK
TVFSAVIEKPPSEQKLVTALQKVVNWRPKERKK"
gene complement(<201367..>203100)
/gene="SIS2"
/locus_tag="AWJ20_3766"
/db_xref="GeneID:30035823"
mRNA complement(<201367..>203100)
/gene="SIS2"
/locus_tag="AWJ20_3766"
/product="phosphopantothenoylcysteine decarboxylase
complex subunit SIS2"
/transcript_id="XM_018880794.1"
/db_xref="GeneID:30035823"
CDS complement(201367..203100)
/gene="SIS2"
/locus_tag="AWJ20_3766"
/note="Negative regulatory subunit of protein phosphatase
1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of
phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p,
Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein
Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and
Vhs3p); SIS2 has a paralog, VHS3, that arose from the
whole genome duplication; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IC,IPI] [PMID 9636153]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 7705654];
GO_component: GO:0005634 - nucleus [Evidence IPI] [PMID
9636153]; GO_component: GO:0071513 -
phosphopantothenoylcysteine decarboxylase complex
[Evidence IDA] [PMID 19915539]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0004633
- phosphopantothenoylcysteine decarboxylase activity
[Evidence ISS] [PMID 10592175]; GO_function: GO:0004633 -
phosphopantothenoylcysteine decarboxylase activity
[Evidence IDA,IMP] [PMID 19915539]; GO_function:
GO:0004864 - protein phosphatase inhibitor activity
[Evidence IEA]; GO_function: GO:0004864 - protein
phosphatase inhibitor activity [Evidence IDA] [PMID
9636153]; GO_process: GO:0015937 - coenzyme A biosynthetic
process [Evidence IEA]; GO_process: GO:0015937 - coenzyme
A biosynthetic process [Evidence ISS] [PMID 10592175];
GO_process: GO:0043086 - negative regulation of catalytic
activity [Evidence IEA]; GO_process: GO:0007346 -
regulation of mitotic cell cycle [Evidence IGI] [PMID
10022927]; GO_process: GO:0009651 - response to salt
stress [Evidence IGI,IMP] [PMID 9636153]"
/codon_start=1
/product="phosphopantothenoylcysteine decarboxylase
complex subunit SIS2"
/protein_id="XP_018733449.1"
/db_xref="GeneID:30035823"
/translation="MSEVGSDQNTLAQQDQFRHQQQQQQQPQQQQDQQKEQQVPSLKT
DSIGETGLIGASGVATTNTGIRPPASTQSSADGTAKAKKEKPNFKLDIMPPSYFQQQS
QNASQQAPAESILVKDTSRLSIDSGRAKGATLLPPPNNSTSHNKQPSMHAHFLVNDVV
GHQGNSRSRSASGSDRGSDSDFARSAVIESIIKGENKGLNISGGPAGSSNTSLNASRS
GSVSGAASGGPSNATSGTSSTHNNGSVAIGGVVSGGNNNGLNLDSTLKSRQGSTVDAI
GPDSVVGTLDGIPASTAAGAAQTTASATSNSDPRLPQDDGRIHILIGATGSVSTGKLR
QIIHKLEEIYTKPKVSIQVILTKAAEKFVSRGEIPSNIRIWRDSDEWANWRGRSDAVV
HIELRRWADILVVAPLSANTLGKIAIGLCDNLLTNVIRAWDTQYPILIAPAMSSYAYN
HPATKRHIKTIKEEMKWIETLKPVEKVVGSYGGIGMGGMMDWNEIVDKTIMKLGGYPE
NDLEDEDDESIDRTKDDDDDDDDDDDDEDDDEDDDDDNDGQTKRDDRARDVDSDDDTD
SRRRRNSVSSF"
gene <206522..>208168
/gene="PHO80"
/locus_tag="AWJ20_3767"
/db_xref="GeneID:30035824"
mRNA <206522..>208168
/gene="PHO80"
/locus_tag="AWJ20_3767"
/product="Pho80p"
/transcript_id="XM_018880795.1"
/db_xref="GeneID:30035824"
CDS 206522..208168
/gene="PHO80"
/locus_tag="AWJ20_3767"
/inference="similar to AA sequence:KEGG_Orthology:K06654"
/note="Cyclin; interacts with cyclin-dependent kinase
Pho85p; regulates the response to nutrient levels and
environmental conditions, including the response to
phosphate limitation and stress-dependent calcium
signaling; GO_component: GO:0000307 - cyclin-dependent
protein kinase holoenzyme complex [Evidence IPI] [PMID
8108735]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 9853758]; GO_function: GO:0016538 -
cyclin-dependent protein serine/threonine kinase regulator
activity [Evidence IDA] [PMID 8108735]; GO_function:
GO:0019901 - protein kinase binding [Evidence IEA];
GO_process: GO:0006875 - cellular metal ion homeostasis
[Evidence IMP] [PMID 20429018]; GO_process: GO:0050849 -
negative regulation of calcium-mediated signaling
[Evidence IGI] [PMID 16455487]; GO_process: GO:0016242 -
negative regulation of macroautophagy [Evidence IMP] [PMID
20417603]; GO_process: GO:0045936 - negative regulation of
phosphate metabolic process [Evidence IGI] [PMID 3915785];
GO_process: GO:0000122 - negative regulation of
transcription from RNA polymerase II promoter [Evidence
IGI] [PMID 3915785]; GO_process: GO:0000079 - regulation
of cyclin-dependent protein serine/threonine kinase
activity [Evidence IEA]; GO_process: GO:0000079 -
regulation of cyclin-dependent protein serine/threonine
kinase activity [Evidence IDA] [PMID 8108735]; GO_process:
GO:0032880 - regulation of protein localization [Evidence
IMP] [PMID 16455487]"
/codon_start=1
/product="Pho80p"
/protein_id="XP_018733450.1"
/db_xref="GeneID:30035824"
/translation="MSGPGNSDSVNGALAAISVPATAPVTASGSEEHIQQKGHVPETA
STSAGLGGATDSTAPRTGAHDLSQNGKGVSAEAVNGASVVNQQQQLLQQECQRQEQQQ
EEQQIQQIQQQQQQQQQQQQQQLQQQQHQQQQTSSQTTHVATPSTPLSPRNVKFVSRN
FYDCPMNDLVRLVSSMLQELVSLNDALPFKTDQLTRFHSRSAPGISIQDYLIRIIKFC
TLEKSIVLSIIYFIDLLCTTYSKFNINSLTVHRFMITAAMVGSKGLCDSFCTNSHYAK
VGGVSKVELNLLEVEFLTRVDYRIVPKVELLNQYYESMVARLEGVYAFPAEPSPASAL
VSSPPHAGLTPPPFDHDVPSAPATFNGGNGSNTTPTHYSFGHLSHISTPNRASSQPPE
FTQTPTAQSNPISDSNMTNSAHHHRSVKVTLREAALSLSKLMLPSDIARKEGRKNRSS
VLKRRFSSSFSGSNNSAPDPVTTQSNETDLATVVENDMPVAPAVPADITASLMYDPEG
YPSPRKKPNIIDSVPSGPTDVFDEADVDLPMTPKRPKPTP"
gene <209713..>210915
/gene="ECT1"
/locus_tag="AWJ20_3768"
/db_xref="GeneID:30035825"
mRNA <209713..>210915
/gene="ECT1"
/locus_tag="AWJ20_3768"
/product="ethanolamine-phosphate cytidylyltransferase"
/transcript_id="XM_018880796.1"
/db_xref="GeneID:30035825"
CDS 209713..210915
/gene="ECT1"
/locus_tag="AWJ20_3768"
/inference="similar to AA sequence:KEGG_Orthology:K00967"
/note="Ethanolamine-phosphate cytidylyltransferase;
catalyzes the second step of phosphatidylethanolamine
biosynthesis; involved in the maintenance of plasma
membrane; similar to mammalian CTP: phosphocholine
cytidylyl-transferases; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003824 - catalytic activity [Evidence
IEA]; GO_function: GO:0004306 - ethanolamine-phosphate
cytidylyltransferase activity [Evidence IEA]; GO_function:
GO:0004306 - ethanolamine-phosphate cytidylyltransferase
activity [Evidence IDA,IMP,ISS] [PMID 8982874];
GO_function: GO:0016779 - nucleotidyltransferase activity
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0009058 -
biosynthetic process [Evidence IEA]; GO_process:
GO:0006629 - lipid metabolic process [Evidence IEA];
GO_process: GO:0006646 - phosphatidylethanolamine
biosynthetic process [Evidence IEA]; GO_process:
GO:0006646 - phosphatidylethanolamine biosynthetic process
[Evidence IMP] [PMID 8982874]; GO_process: GO:0008654 -
phospholipid biosynthetic process [Evidence IEA]"
/codon_start=1
/product="ethanolamine-phosphate cytidylyltransferase"
/protein_id="XP_018733451.1"
/db_xref="GeneID:30035825"
/translation="MTHSEINSTETTSTPLREDRYWVDGCFDFTHHGHAGAIRQARKL
ANELYVGIHTDEEIYVNKGPVVMKLEERVTAVEACKWCTKSVPGAPYVTDPEVLDSYG
CKYVVHGDDITTDADGNDCYQVVKDMGRFVVVKRTPNISTTDLVDRMLSSRTTHHIES
ITPSNYSSHVLINDEDSLTRFKDYATAVDAKAPWSGVYVSVDDKTSVLVEPAAGVADK
LKKNAFYVDGGFDLFFMGHIEFLRLVHEEAQRSNAVVVVGLHDDATVNSVKGENYPIM
NQFERALCVLQCKYVDSVVIGAPFTPDEPFINSLSPHMTIKQVLHGPTPIVGEKGTKT
VDPYVYVKEQGIYKQIGPHKYDDVSSNKIVDRVLEYREQYEERQRKKGVKAVNERDLE
AIEKATAK"
gene <214505..>217816
/gene="CHS1"
/locus_tag="AWJ20_3769"
/db_xref="GeneID:30035826"
mRNA <214505..>217816
/gene="CHS1"
/locus_tag="AWJ20_3769"
/product="chitin synthase CHS1"
/transcript_id="XM_018880797.1"
/db_xref="GeneID:30035826"
CDS 214505..217816
/gene="CHS1"
/locus_tag="AWJ20_3769"
/inference="similar to AA sequence:KEGG_Orthology:K00698"
/note="Chitin synthase I; requires activation from
zymogenic form in order to catalyze the transfer of
N-acetylglucosamine (GlcNAc) to chitin; required for
repairing the chitin septum during cytokinesis;
transcription activated by mating factor; GO_component:
GO:0045009 - chitosome [Evidence IDA] [PMID 8970154];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 8970154]; GO_function: GO:0004100 -
chitin synthase activity [Evidence IEA,IEA]; GO_function:
GO:0004100 - chitin synthase activity [Evidence IDA] [PMID
7952171]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_function: GO:0016757 - transferase
activity, transferring glycosyl groups [Evidence IEA];
GO_function: GO:0016758 - transferase activity,
transferring hexosyl groups [Evidence IEA]; GO_process:
GO:0006031 - chitin biosynthetic process [Evidence IEA];
GO_process: GO:0007109 - cytokinesis, completion of
separation [Evidence IMP] [PMID 2523889]"
/codon_start=1
/product="chitin synthase CHS1"
/protein_id="XP_018733452.1"
/db_xref="GeneID:30035826"
/translation="MTPNTGPESSGGHAHNQYYSYDDNPDPYGHQDQEEGDRVPLNPS
GGPDGLGHMPSPNPYQGGMDQSGMGFGNHDVHGGPPGGPQGYAEDDQYYEYPIGQQHH
EPVDNQYYDPYAHTAATPGVGVGPIPGMNPMNHVPPPMPGPPPAPIPPPQPAMPYDTH
PINPYSNPPTNPYGNPFENEPAFESQYNLPQEPESYLLKPVYPPPQPLHHAMDTNHSP
FSSYFGSTNDDVDDFHPFPVQGDQYALNTYEDDDDTEDSDSSDGRDDSFAYQPPVAPQ
PIPAGAAVSGMPLPSGPTPPPIMGAPLPVAPPPNMQPRRAKTMKRVRLFKGNLVLDCP
VSGTLLSQFPDDLEGQREFTHMRYSAATCDPKDFKLNGFTMRQNCYTHPRATELFIVI
TIYNESDILLGKTLQGVFRNIKHLSSRTRSKTWGKDAWKKVVVCVVADGREKLNPRAR
ALMAALGVYQDGLAKNMVNDRPVDAHIYEYTTRVGISHVDTTVKLVTEKTVPVQMIFC
LKEHNKKKINSHRWFFQAFGPILNPNICVLLDAGTQPGHDSIYHLWKAFDVNPHVGGA
CGEITAGLGTGFRKLLNPLVAAQNFEYKMSNILDKPLESVFGFISVLPGAFSAYRYVA
LQNEPLEKYFKGETLHDSGAGIFTANMYLAEDRILCYELVAKRGDRWLLKYVKSAHAE
TDVPDKLDELVLQRRRWLNGSFFAAVYAQVHMFAIWRSSHSILRKLSLHLEFLYQMVS
MLFSWFSIGNYFLVFRILTSSLSDSSLGFAPGKILSVLFLWIYAGCLVTIFVLSFGNR
PSGTAHFYYAIVAFFAFLMAYLMFAAIYISVKSVEYAICANGGFSPALVVKNQTFRDL
VISILSTYALYFISSFLFFEPWHMFTSFLQYLLISPSYINVLNVYAFCNIHDISWGTK
GDTSQKMDLGVAKLKTTQSKKEILEVDVPTSKEEINSSYLNQISLLRDEAKEEKKVVS
KEDKNIDYYAQFRSAVVLSWIFTNVALVAVVLNTAGLNVFNSNSNSSNPNSGTLKRGL
DMGIELLKRQTPAAPASPAPAAGAGSGSCSSIGVGEDVQTQIYLSVILWSVAALAAFR
FIGSVWYLVLRLMGR"
gene <218027..>218737
/gene="dtd"
/locus_tag="AWJ20_3770"
/db_xref="GeneID:30035828"
mRNA <218027..>218737
/gene="dtd"
/locus_tag="AWJ20_3770"
/product="D-tyrosyl-tRNA(Tyr) deacylase"
/transcript_id="XM_018880799.1"
/db_xref="GeneID:30035828"
CDS 218027..218737
/gene="dtd"
/locus_tag="AWJ20_3770"
/note="Fungal Genetics Stock Center 12543"
/codon_start=1
/product="D-tyrosyl-tRNA(Tyr) deacylase"
/protein_id="XP_018733453.1"
/db_xref="GeneID:30035828"
/translation="MLVYSRFALRRAYTSKCIHEKLFHTSRTLLSEVNPVYPDDSHLT
KIKKPGMLFSEPIDPNLRMGYNPLYSSPVGGQIAFTKRWSIGLSLIGSYVGYLVHDVT
GASDLAGIIGAAVLIAPLPLVQFFAGNYVTNIYRLYRKDEPQTYENLTKDETIVLERI
GLFGRKTYATAVKVQDLRLVNKRFGWVNWEYKDPKTNFSTKFYVADNIGGMKMDRIWG
IIEKNSGVDNGRSFLNEP"
gene <222301..>223590
/locus_tag="AWJ20_3771"
/db_xref="GeneID:30035829"
mRNA <222301..>223590
/locus_tag="AWJ20_3771"
/product="hypothetical protein"
/transcript_id="XM_018880800.1"
/db_xref="GeneID:30035829"
CDS 222301..223590
/locus_tag="AWJ20_3771"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733454.1"
/db_xref="GeneID:30035829"
/translation="MTQVSYLRALIVGAGSRGRAYTRSLRHLDYIRVTGVAEPSGPRR
KKLINYCHQVYPDKVAITEYSDWKESLADSGNYDCVIICVLDDMHREVAVAFANAGKH
MLCEKPLATTLEDCEAIVKAVKENNVLLAIGHVLRYSPHNIALKELLDSGVVGEIINI
NHTEPVGWYHFAHSYVRGNWRREDTTTFSLMAKCCHDIDVLMWLLGYENLRRVSSFGS
LSYFKKENKPEAAGDRCMSCPIEESCAYSAKKIYYDDLAAGRKAWKTKTITDIEDPVH
LKKALEEGPYGICVFDADNDVCDNQVVSLDFGKATATMTMIAFSEEVCFRKMCIYGTK
GEISTDSRTIKLFDFATRTTKSFTPDTDEESGHGGGDKGLATAFAEALKDVLLHGSSI
ENATTKHIKCTPEEVLGSHKVVFLAEQARLNGQVISL"
gene <223899..>226607
/gene="UTP14"
/locus_tag="AWJ20_3772"
/db_xref="GeneID:30035830"
mRNA <223899..>226607
/gene="UTP14"
/locus_tag="AWJ20_3772"
/product="Utp14p"
/transcript_id="XM_018880801.1"
/db_xref="GeneID:30035830"
CDS 223899..226607
/gene="UTP14"
/locus_tag="AWJ20_3772"
/inference="similar to AA sequence:KEGG_Orthology:K14567"
/note="Subunit of U3-containing Small Subunit (SSU)
processome complex; involved in production of 18S rRNA and
assembly of small ribosomal subunit; GO_component:
GO:0005730 - nucleolus [Evidence IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 12068309];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0032040 - small-subunit
processome [Evidence IEA]; GO_component: GO:0032040 -
small-subunit processome [Evidence IDA] [PMID 12068309];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_process: GO:0000480 - endonucleolytic
cleavage in 5'-ETS of tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15489292]; GO_process: GO:0000447 - endonucleolytic
cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and
LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000472 - endonucleolytic cleavage to
generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0006364 - rRNA processing [Evidence
IEA,IEA]; GO_process: GO:0042254 - ribosome biogenesis
[Evidence IEA]"
/codon_start=1
/product="Utp14p"
/protein_id="XP_018733455.1"
/db_xref="GeneID:30035830"
/translation="MAKKGVKKRQNRRALSNRAQNAFNLAQDEEDSRLMRGMEPDIEN
DSDREGEDGEKPAYRIGEVASEDDEDIDSDEALGSDDEEIFESHRRKSNKNDDDDEED
SDDDGYKSVDESEFVSLSEVWDLDDKDERETSGKDKGSSKERLPNKIDENNLLKLDDE
ILSDSESEADSESQDDSESEGGSDSEGGSSGSDSVLSDEFSDLDEDDIDEEKLANLKN
MISGMSEEALAEATRKAKKQRLQMMNIQESEFALPVGGQKLSLSDLTAGVTTEKKQDS
GLKLLENNSKQTLAVPLAKRIQQKNDRKAAYELTKEEVSKWEETVKKNREAEHLQFPI
NPAPQVSKVAAHAPIEPSTELEKKVNNLLTESNLSEEKSVSTFEELAPSKLTMEEVKR
RRNELRLMRELMFREEQKARRIKKIKSKSYRKVHKKERERERMMVEGDEESDRESHDI
ERARERMSLKHKNTGKWAKRMVEQGFTKDKETRGEMEEMLRRGEDLRKKILDDQDNES
DSQEIESAGDDDDDEDDETINEQKESLGKGLLAMKFMKDAEQKERQINKAQREALRNA
KNLDDFEDLKDETEDAVNQVVNAGRRKFAPGTSEARDEMLDELEKARDETIIDEEKSL
ENVITKAFDSRTNSRTKSKSKHSKSQEPDEAEEEEANPWLTISSNSKQKSKKVNIVDK
NSSTQSKSQDKLRKEKEKLRSSSNDDSGDIDMNEVLRIVDPLASDNEEGDDDASEAVA
EGQNDMKRVPRKGLTFKQRELVKRAFAGDDVVEEFQEEKRQKIADEGDKEIDVTLPGW
GSWGGSSIKKKKKVIKKVQGIQADKRQDARLKNVIINEKVNKKSSKYNASSVPFPYEN
REQYERSLRMPIGREWSSRDTFQKLTKPRVIVKQGTVIDPIKAPFK"
gene <232657..>235095
/gene="XBP1"
/locus_tag="AWJ20_3773"
/db_xref="GeneID:30035831"
mRNA <232657..>235095
/gene="XBP1"
/locus_tag="AWJ20_3773"
/product="Xbp1p"
/transcript_id="XM_018880802.1"
/db_xref="GeneID:30035831"
CDS 232657..235095
/gene="XBP1"
/locus_tag="AWJ20_3773"
/note="Transcriptional repressor; binds to promoter
sequences of the cyclin genes, CYS3, and SMF2; expression
is induced by stress or starvation during mitosis, and
late in meiosis; member of the Swi4p/Mbp1p family;
potential Cdc28p substrate; relative distribution to the
nucleus increases upon DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
22842922]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 22842922]; GO_component: GO:0005634 - nucleus
[Evidence IDA,IPI] [PMID 9343412]; GO_function: GO:0003677
- DNA binding [Evidence IEA,IEA]; GO_function: GO:0043565
- sequence-specific DNA binding [Evidence IDA] [PMID
19111667]; GO_function: GO:0043565 - sequence-specific DNA
binding [Evidence IDA] [PMID 19158363]; GO_function:
GO:0003700 - sequence-specific DNA binding transcription
factor activity [Evidence IEA]; GO_function: GO:0003700 -
sequence-specific DNA binding transcription factor
activity [Evidence IDA] [PMID 9343412]; GO_process:
GO:0042149 - cellular response to glucose starvation
[Evidence IMP] [PMID 9343412]; GO_process: GO:0034605 -
cellular response to heat [Evidence IMP] [PMID 9343412];
GO_process: GO:0034599 - cellular response to oxidative
stress [Evidence IMP] [PMID 9343412]; GO_process:
GO:0071472 - cellular response to salt stress [Evidence
IMP] [PMID 9343412]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA,IEA];
GO_process: GO:0006950 - response to stress [Evidence
IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Xbp1p"
/protein_id="XP_018733456.1"
/db_xref="GeneID:30035831"
/translation="MSWTPFNGPSGQHEPHGLSDASNQLNGPRRPLPLDSQRAGDASN
QSIQYTRSPQTVPLIAPARNVHGRDLLNPTGSSGTGVAPAQPGQHPPSPDLESTSHSD
LHLQHQQQHQQQHPYQPSHQRLGSISYQQEHISNQELHLHHQKQNSHQLTGHQNSHIL
PSHPRQHLDHQLDHQLDHQHHLHVHGHGPLAVGDSSHAHILPNITGNGHNGHHKSHSD
MTNMTRAGQITPPPGHNMSGVAVSASSPLPSSASLSAKPHGLANNRKQSLASSSPKAP
PKQRVSRTSHLEPANSPGFPVNYPIYPMVDIGTIPVQQVPLLPLPGSIVMKRYATSVD
QRNFLTVYEYMVNNQWVIWDYFTGYVHLTGLWKAVGNSKADIVRLVDNSPDLEPVIKR
VRGGFLKIQGTWLPYSVARTLASRTCYHIRYALIPIFGPDFPESCLQPHQPGFGQLQL
TTTENPRRRRKRSSVGSISGITGLTSVSTANGVSNGAGAGTGGPGAGELSPNPQSPVQ
RKRRQSELAPSTQPFNSHGLASPRSSHASIMNPVLITPAGSKNDNQRQGMSDSNRIMS
LSMTSSSGSTATAGLSPLRHHRHSKSIGSIDTSSLAIGGSIVPRLELNRPSYFKRPAY
DEDSQAIEFSSSSDDDDDYEAHLVKRAKHDHSDSIHDTTGRTSKPASGSGPVQVPHSP
HHRYILPPPMPVKVVFADETELVESPDEFLAVLQATRSLQQLSAGKAGRRWSLNPAST
TASGGSFECGGKLWRWDGGQKLTSTGLAADGDSSPKSQVVERVKSLPDRRYSHGDEPI
RQRSNVMNINGLIS"
gene complement(<235754..>236602)
/gene="RSM10"
/locus_tag="AWJ20_3774"
/db_xref="GeneID:30035832"
mRNA complement(<235754..>236602)
/gene="RSM10"
/locus_tag="AWJ20_3774"
/product="mitochondrial 37S ribosomal protein RSM10"
/transcript_id="XM_018880803.1"
/db_xref="GeneID:30035832"
CDS complement(235754..236602)
/gene="RSM10"
/locus_tag="AWJ20_3774"
/inference="similar to AA sequence:KEGG_Orthology:K02946"
/note="Mitochondrial ribosomal protein of the small
subunit; has similarity to E. coli S10 ribosomal protein;
essential for viability, unlike most other mitoribosomal
proteins; GO_component: GO:0005763 - mitochondrial small
ribosomal subunit [Evidence IPI] [PMID 11278769];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA,IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IEA]; GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IPI] [PMID 11278769]; GO_process:
GO:0032543 - mitochondrial translation [Evidence IC] [PMID
11278769]; GO_process: GO:0006412 - translation [Evidence
IEA]"
/codon_start=1
/product="mitochondrial 37S ribosomal protein RSM10"
/protein_id="XP_018733457.1"
/db_xref="GeneID:30035832"
/translation="MNSVRFLAAQCRLATRGVYTPAVRGFRSVSAANSASTPASSQPA
SEPVRARMSPSGPYVDDTESYSSEHLYPKEGEYVQSSTEEAYGTGRPVPINVELNHYA
PIKHKLTHGHRVADVHLRSFTTQNLDFYADFLLRVAFYLRIPVKGVTPLPNKTEKWTV
IRSPFAHAKSKENFQRITHKRLIQLYDANPEVVQVFLATAREYSIGGVGVKATLYHKE
GLGVIDKLDLPETGAESSSLNPLSLENVDLGSADSEVARAVLDILEDPVFKPLMDEAK
PTQKAE"
gene <237120..>237908
/gene="BMT2"
/locus_tag="AWJ20_3775"
/db_xref="GeneID:30035833"
mRNA <237120..>237908
/gene="BMT2"
/locus_tag="AWJ20_3775"
/product="Bmt2p"
/transcript_id="XM_018880804.1"
/db_xref="GeneID:30035833"
CDS 237120..237908
/gene="BMT2"
/locus_tag="AWJ20_3775"
/inference="similar to AA sequence:KEGG_Orthology:K18849"
/note="Nucleolar S-adenosylmethionine-dependent rRNA
methyltransferase; methylates adenine (m1A) of the large
subunit (LSU) rRNA at position 2142; belongs to Rossmann
fold superfamily; green fluorescent protein (GFP)-fusion
protein localizes to the nucleolus; YBR141C is not an
essential gene; GO_component: GO:0005730 - nucleolus
[Evidence IEA,IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 23558746]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_function:
GO:0008757 - S-adenosylmethionine-dependent
methyltransferase activity [Evidence ISM] [PMID 19351663];
GO_function: GO:0008168 - methyltransferase activity
[Evidence IEA]; GO_function: GO:0016433 - rRNA (adenine)
methyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0016433 - rRNA (adenine) methyltransferase
activity [Evidence IMP] [PMID 23558746]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0008150 - biological_process [Evidence ND];
GO_process: GO:0032259 - methylation [Evidence IEA];
GO_process: GO:0031167 - rRNA methylation [Evidence
IEA,IEA]"
/codon_start=1
/product="Bmt2p"
/protein_id="XP_018733458.1"
/db_xref="GeneID:30035833"
/translation="MMKRQASLQAQLDKTIDPAERAGLQDEISRISKALNEEGGLDKY
QQASIAGQSTTRGGDSSKILVDWLKDELKTEDQLRTDLSLLEVGCLSVKNACSQSKLF
SSIERIDLNSQDPLIKKQDFMERPLPRQNQKETDRFDVISLSLVLNYVPDAVTRGQML
YRTTQFLKPPSTPGRPLPALFLVLPSPCTNNSRYLTEERLLQILQTLGYTLTQRKEAK
KVVYWLLTLEKPPLTPSQAAKLKGRFKKKLLNDGKIRNNFAVVI"
gene complement(<238833..>240815)
/gene="AVT1"
/locus_tag="AWJ20_3776"
/db_xref="GeneID:30035834"
mRNA complement(<238833..>240815)
/gene="AVT1"
/locus_tag="AWJ20_3776"
/product="Avt1p"
/transcript_id="XM_018880805.1"
/db_xref="GeneID:30035834"
CDS complement(238833..240815)
/gene="AVT1"
/locus_tag="AWJ20_3776"
/inference="similar to AA sequence:KEGG_Orthology:K15015"
/note="Vacuolar transporter; imports large neutral amino
acids into the vacuole; member of a family of seven S.
cerevisiae genes (AVT1-7) related to vesicular
GABA-glycine transporters; GO_component: GO:0000324 -
fungal-type vacuole [Evidence IDA] [PMID 11274162];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IMP] [PMID 11274162]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005774 -
vacuolar membrane [Evidence IEA]; GO_component: GO:0005773
- vacuole [Evidence IEA]; GO_function: GO:0015186 -
L-glutamine transmembrane transporter activity [Evidence
IMP] [PMID 11274162]; GO_function: GO:0015188 -
L-isoleucine transmembrane transporter activity [Evidence
IMP] [PMID 11274162]; GO_function: GO:0005302 - L-tyrosine
transmembrane transporter activity [Evidence IMP] [PMID
11274162]; GO_function: GO:0005215 - transporter activity
[Evidence ISS] [PMID 11274162]; GO_process: GO:0006865 -
amino acid transport [Evidence IEA]; GO_process:
GO:0006865 - amino acid transport [Evidence IMP] [PMID
11274162]; GO_process: GO:0006810 - transport [Evidence
IEA]; GO_process: GO:0007034 - vacuolar transport
[Evidence IMP] [PMID 11274162]"
/codon_start=1
/product="Avt1p"
/protein_id="XP_018733459.1"
/db_xref="GeneID:30035834"
/translation="MPGTNSQIPSSPDRNGSAAHGSSAWHGGSSTSGLPSSATYGTPG
LGTGGTTTTGGGIGSGNLTSSRRSSFFNGAGGGLNSIEHFASSYTRAQSFLAIEPPSE
ISRQRSFFDETGSPVIPSSFASRYRPHFDEGTDFTDAIDEDGYESSTYLDDNEDASGH
ISTGGTEPSQTDHLLGHNESLYGSYKSTSTSGGYLNIPTHNRRHPRSRRLSLYTAEPD
QGTHILGESQAISTGTEQDPVIIQKVEDEDGHITTVIAGQSTAPQTVFNSVNILIGIG
LLALPLGFRYAGWVIGCILLVISAASTFYSAKLLAKCLDTDSTIVTYADVAYAAFGSR
ARILVSMLFTIELMGAGVSLVVLFSDSLHSLVPSISQLQWKFVAFAILTPPCFLPLRI
LSFSSIMGITATFALVVIVLFDGFYKADSPGSLLHPATTWMWPEKWSLLPMSIGIFMA
PWGGHAVFPNIYRDMRHPQKYGQCLKTTYKITFLVDASMGVLGFLMFGQDVMDEVTKS
ILLTPGYPESLNYLITLLIATIPLAKTPLNARPIVSTLDILFGIDKVEFGDKTSHHFG
ARIGKFLVRVSVVASFVLMAIAFPDFDRIIAFFGSLLCVTICMILPLSFYLKLYAGRI
SKFELILDYILLVFFSILAIVGTIWCMLPADLIGRE"
gene <242840..>243553
/locus_tag="AWJ20_3777"
/db_xref="GeneID:30035835"
mRNA <242840..>243553
/locus_tag="AWJ20_3777"
/product="hypothetical protein"
/transcript_id="XM_018880806.1"
/db_xref="GeneID:30035835"
CDS 242840..243553
/locus_tag="AWJ20_3777"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733460.1"
/db_xref="GeneID:30035835"
/translation="MILASLELVRLQLPTDPWQQDAARARQQAVRKGQNPSFWFGPTG
YRAVEYKEWKRRMDQALNKAQNVGKSVNLAKEVYSEIRENNRQLSHKILEELKRENEQ
DAQTRENKALIELKEEEGHDIASSSSASTSNSNINSSTSDSRNLNTNNNNNSNGKSAT
GEKSRTDSLSVDSLMSAIESLSTTKSDEWDALEPWEKLRLETDIAVRIMPHTRGVQEQ
AADKSLSINLSLDDDSPST"
gene complement(<243867..>245744)
/locus_tag="AWJ20_3778"
/db_xref="GeneID:30035836"
mRNA complement(<243867..>245744)
/locus_tag="AWJ20_3778"
/product="hypothetical protein"
/transcript_id="XM_018880807.1"
/db_xref="GeneID:30035836"
CDS complement(243867..245744)
/locus_tag="AWJ20_3778"
/inference="similar to AA sequence:KEGG_Orthology:K03510"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733461.1"
/db_xref="GeneID:30035836"
/translation="MSKVVISLDLDAFYCAVEQHYDPTLAGDVPFIVYQKNCVATLSY
AARNLGLKKLSTVSEAVKKVPGIRLVNGESLAKYRAEGKKILDFVSGLFGGCVPMERL
GLEELRIDITKVVDYNMENLRNTGLLDIIRNSGAEVGDESPEGIYLFMTDGDTPTNDS
GISGRRVIGGGGGLDGSEADIDKTPSSDPFFCENFFFNVPAKVFPLDRNNALPTFTDS
EEHLRCYIGGQLASYIMERLSMDMGYNCSIGLGPNKTIAKMACSLNKPKGLTIVLPEH
VQDYLDSTFVSAIPGFGHKTVRRIAEDIAFSDRPGNGLFSTFKRENENPTSAAPIRDS
RTPTPDSPLATNSNEAKLSSVEVSPPTLQKPVSIEASPSSNKPVSVETSPSSKKPIST
IKSSGGVEKLELHALMDREFGRHLTVYDVRTNLHRTDFVRLFGQKQGSVLWDLMHGID
NSPVKQASDIPTQISIEDTYKRIDTFELVKEAIVSLTNSLLYQIHCDLIDSGDLTWRV
FPTTFRLTMSRGDFNPYAVRKSKSTRLPPSFFDEMLSISSTPDPTPLSYEVLSRKLFQ
DPILALYNQLSTARAGEKLNVRLLNVAVTNFDPTRPTATRSKPSSRQSRGVMDRFLNL
K"
gene <247766..>248209
/gene="YPT7"
/locus_tag="AWJ20_3779"
/db_xref="GeneID:30035837"
mRNA <247766..>248209
/gene="YPT7"
/locus_tag="AWJ20_3779"
/product="Rab family GTPase YPT7"
/transcript_id="XM_018880808.1"
/db_xref="GeneID:30035837"
CDS 247766..248209
/gene="YPT7"
/locus_tag="AWJ20_3779"
/inference="similar to AA sequence:KEGG_Orthology:K07897"
/note="Rab family GTPase; GTP-binding protein of the rab
family; required for homotypic fusion event in vacuole
inheritance, for endosome-endosome fusion; interacts with
the cargo selection/retromer complex for retrograde
sorting; similar to mammalian Rab7; GO_component:
GO:0000324 - fungal-type vacuole [Evidence IDA] [PMID
11853670]; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 22593205]; GO_component:
GO:0005741 - mitochondrial outer membrane [Evidence IDA]
[PMID 16407407]; GO_component: GO:0030906 - retromer
complex, inner shell [Evidence IPI] [PMID 22593205];
GO_component: GO:0005773 - vacuole [Evidence IEA,IEA];
GO_function: GO:0005525 - GTP binding [Evidence IEA,IEA];
GO_function: GO:0003924 - GTPase activity [Evidence IDA]
[PMID 11210571]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006896 - Golgi to
vacuole transport [Evidence TAS] [PMID 9714809];
GO_process: GO:0006897 - endocytosis [Evidence IMP] [PMID
1473149]; GO_process: GO:0034727 - piecemeal
microautophagy of nucleus [Evidence IMP] [PMID 18701704];
GO_process: GO:0072665 - protein localization to vacuole
[Evidence IMP] [PMID 22593205]; GO_process: GO:0015031 -
protein transport [Evidence IEA,IEA]; GO_process:
GO:0032889 - regulation of vacuole fusion, non-autophagic
[Evidence IMP] [PMID 11598008]; GO_process: GO:0032889 -
regulation of vacuole fusion, non-autophagic [Evidence
IMP] [PMID 7489715]; GO_process: GO:0042147 - retrograde
transport, endosome to Golgi [Evidence IPI] [PMID
22593205]; GO_process: GO:0007264 - small GTPase mediated
signal transduction [Evidence IEA]; GO_process: GO:0006810
- transport [Evidence IEA]; GO_process: GO:0000011 -
vacuole inheritance [Evidence IDA] [PMID 7489715];
GO_process: GO:0016192 - vesicle-mediated transport
[Evidence IDA] [PMID 9015302]"
/codon_start=1
/product="Rab family GTPase YPT7"
/protein_id="XP_018733462.1"
/db_xref="GeneID:30035837"
/translation="MQIWDTAGQERFQSLGVAFYRGADCCVLMYDVNNAKSFEALENW
RDEFLIQANPRDPDSFPFVVIGNKIDVEDSKRAVSTKRAQAFCASKGNLPYFETSAKE
ATGVEQAFEVVARNALQQEDAQDFSQEYSDAININLDSDSSSCAC"
gene <250075..>252407
/gene="ACS2"
/locus_tag="AWJ20_3780"
/db_xref="GeneID:30035839"
mRNA join(<250075..250118,250484..>252407)
/gene="ACS2"
/locus_tag="AWJ20_3780"
/product="acetate--CoA ligase ACS2"
/transcript_id="XM_018880810.1"
/db_xref="GeneID:30035839"
CDS join(250075..250118,250484..252407)
/gene="ACS2"
/locus_tag="AWJ20_3780"
/inference="similar to AA sequence:KEGG_Orthology:K01895"
/note="Acetyl-coA synthetase isoform; along with Acs1p,
acetyl-coA synthetase isoform is the nuclear source of
acetyl-coA for histone acetylation; mutants affect global
transcription; required for growth on glucose; expressed
under anaerobic conditions; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005829 -
cytosol [Evidence IDA] [PMID 16857587]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 9711835];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
19618123]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 16857587]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 19618123]; GO_function: GO:0016208 -
AMP binding [Evidence IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0003987 -
acetate-CoA ligase activity [Evidence IEA,IEA];
GO_function: GO:0003987 - acetate-CoA ligase activity
[Evidence IDA] [PMID 8910545]; GO_function: GO:0016880 -
acid-ammonia (or amide) ligase activity [Evidence IDA]
[PMID 18305111]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016874 - ligase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006085
- acetyl-CoA biosynthetic process [Evidence IDA] [PMID
8910545]; GO_process: GO:0019427 - acetyl-CoA biosynthetic
process from acetate [Evidence IEA]; GO_process:
GO:0016573 - histone acetylation [Evidence IMP] [PMID
16857587]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0006090 - pyruvate
metabolic process [Evidence IEA]; GO_process: GO:0001302 -
replicative cell aging [Evidence IMP] [PMID 19618123]"
/codon_start=1
/product="acetate--CoA ligase ACS2"
/protein_id="XP_018733463.1"
/db_xref="GeneID:30035839"
/translation="MTALLRQGCYSKLWRKHPSKTHLSSFDHYSQLYKESIENPDKFF
GDAARELLSWDRDFKTVSSGGFEHGDVAWFLEGQLNASYNCVDRHAFANPEKIALIYE
ADDAKDSRNVTYGELLRQVSKLAAVLTSFGVKKGDTVAIYLPNIPEAIVALLAVTRIG
AIHSVIFAGFSSGSLKDRINDAKCKVIITSDEGRRGGKTVYTKKIVDDSLKNAPSIEK
VLVFKRTGSEIPWTEGRDFWWHEELDKQRAYSPPVAVNSEDPLFLLYTSGSTGSPKGV
VHSTAGYLLGAAITAKHVFDIQEKDILFTAGDVGWITGHTYALYAPLLLGATSVIFEG
TPAYPNLSRYWQIIEKYKVTQFYVAPTALRLLKRGGEEFITGHDLSSLRTLGSVGEPI
AGEIWEWYHELIGNSECHIADTYWQTETGSHIIAPLAGITPMKPGSASFPFFGIDLAI
IDPVTGKELVGNDVEGVLAVKKPWPSIARSVWGAHQRYLDTYLKPYPGLYFTGDGAAR
DHEGFYWIRGRVDDVVNVSGHRLSTAEIEASLIEHHSVAESAVVGVSDDLTGQAVIAF
VSLKDGFDHTQEIKKELIMHVRKEIGPFAAPKTVIVIDDLPKTRSGKIMRRILRKIVS
NEADSIGDISTLNNPQSVDQIIAAVDIQLKK"
gene <253907..>254410
/locus_tag="AWJ20_3781"
/db_xref="GeneID:30035840"
mRNA <253907..>254410
/locus_tag="AWJ20_3781"
/product="Usp (universal stress protein) family protein"
/transcript_id="XM_018880811.1"
/db_xref="GeneID:30035840"
CDS 253907..254410
/locus_tag="AWJ20_3781"
/codon_start=1
/product="Usp (universal stress protein) family protein"
/protein_id="XP_018733464.1"
/db_xref="GeneID:30035840"
/translation="MALEERQYRFEGERFLRQILAKNVNNKKLSLIVEFPVGKIEDMI
QRMIQIYEPAILVVGTRGRSMDGFKGLLLPGSVSKYCLQHSPVPVIVVRGIQKLAKRK
AKRDADPARRAYREMLYQAQLPAMKLYPQGSTGKQLAPTIPAFLRPDHYGSRSATPSR
PSTPAGH"
gene complement(<254505..>255671)
/gene="RAD10"
/locus_tag="AWJ20_3782"
/db_xref="GeneID:30035841"
mRNA complement(<254505..>255671)
/gene="RAD10"
/locus_tag="AWJ20_3782"
/product="Rad10p"
/transcript_id="XM_018880812.1"
/db_xref="GeneID:30035841"
CDS complement(254505..255671)
/gene="RAD10"
/locus_tag="AWJ20_3782"
/inference="similar to AA sequence:KEGG_Orthology:K10849"
/note="Single-stranded DNA endonuclease (with Rad1p);
cleaves single-stranded DNA during nucleotide excision
repair and double-strand break repair; subunit of
Nucleotide Excision Repair Factor 1 (NEF1); homolog of
human ERCC1 protein; GO_component: GO:0000110 -
nucleotide-excision repair factor 1 complex [Evidence IPI]
[PMID 8621533]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 9852079]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0003684 -
damaged DNA binding [Evidence IEA]; GO_function:
GO:0004519 - endonuclease activity [Evidence IEA,IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0004518 - nuclease activity
[Evidence IEA]; GO_function: GO:0003697 - single-stranded
DNA binding [Evidence IDA] [PMID 1741062]; GO_function:
GO:0000014 - single-stranded DNA endodeoxyribonuclease
activity [Evidence IDA] [PMID 8253764]; GO_process:
GO:0006277 - DNA amplification [Evidence IMP] [PMID
23271978]; GO_process: GO:0006281 - DNA repair [Evidence
IEA,IEA]; GO_process: GO:0000733 - DNA strand renaturation
[Evidence IDA] [PMID 1741062]; GO_process: GO:0006974 -
cellular response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0000736 - double-strand break repair via
single-strand annealing, removal of nonhomologous ends
[Evidence IMP] [PMID 7891718]; GO_process: GO:0000710 -
meiotic mismatch repair [Evidence IMP] [PMID 11514439];
GO_process: GO:0006312 - mitotic recombination [Evidence
IMP] [PMID 2188090]; GO_process: GO:0006296 -
nucleotide-excision repair, DNA incision, 5'-to lesion
[Evidence IDA] [PMID 7559571]; GO_process: GO:0000735 -
removal of nonhomologous ends [Evidence IMP] [PMID
7891718]"
/codon_start=1
/product="Rad10p"
/protein_id="XP_018733465.1"
/db_xref="GeneID:30035841"
/translation="MEADRTAAILKAAAALRGKSGNNNGSSNSNGASSGSAPPSNQAT
PGGSNSNSAPAPPLATSTVARTGIQISGTNIPILNSRQTGASATSTSYSSGSSTAANG
SGSGPSKRSYGASSVPGPSGHYRAANSGMSTYSRASPTTSSSLPTNTTSASSATPGST
STAKANTTSSIIVNAKKQKQNPVLKHIRGVGYYMDDIVPDFITGHNSCALFLSIKYHN
IHKEYIYKRIQQLNKAFEHRVLLVLVDVDNPAAALLELDIISLRAGLQMIITWNMEEA
GKYLVLLKQLENASATGISGQKKESYKEQLIEVMNRISRSSINKTDAVTLVANFGSLK
NAILDGGDNVELIHGWGSTKAKVFRDAINEPFINHRQYDMKPYKSESQVDSELP"
gene <258775..>259539
/locus_tag="AWJ20_3783"
/db_xref="GeneID:30035842"
mRNA <258775..>259539
/locus_tag="AWJ20_3783"
/product="hypothetical protein"
/transcript_id="XM_018880813.1"
/db_xref="GeneID:30035842"
CDS 258775..259539
/locus_tag="AWJ20_3783"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733466.1"
/db_xref="GeneID:30035842"
/translation="MSSNTIYFVSGGNRGIGFALVKKLSSLANSVVITTARSPAKATD
LKDWQKEHSNVHVLKYDASVHNDATELATQIEKLTGGIDVFVANSGISEEPMTVLETP
IESWTRLYAINTLGPIVLFKALYPLIAKRDTRKVVFISSGLGSQKNMSDAFGTSSYGQ
SKSALNFTAKELSLELAKENFVVVPVHPGLVNTEMGSGYVDNLGKNSGEFADVLRANV
MITTEQSADGIANVIATLSKEDSGKFLGYDRSTLEW"
gene complement(<261086..>261400)
/gene="DTD1"
/locus_tag="AWJ20_3784"
/db_xref="GeneID:30035843"
mRNA complement(<261086..>261400)
/gene="DTD1"
/locus_tag="AWJ20_3784"
/product="Dtd1p"
/transcript_id="XM_018880814.1"
/db_xref="GeneID:30035843"
CDS complement(261086..261400)
/gene="DTD1"
/locus_tag="AWJ20_3784"
/inference="similar to AA sequence:KEGG_Orthology:K07560"
/note="D-Tyr-tRNA(Tyr) deacylase; functions in protein
translation, may affect nonsense suppression via
alteration of the protein synthesis machinery; ubiquitous
among eukaryotes; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_function:
GO:0097358 - D-leucyl-tRNA(Leu) deacylase activity
[Evidence IMP] [PMID 10918062]; GO_function: GO:0051500 -
D-tyrosyl-tRNA(Tyr) deacylase activity [Evidence IMP]
[PMID 10766779]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0016788 -
hydrolase activity, acting on ester bonds [Evidence IEA];
GO_process: GO:0019478 - D-amino acid catabolic process
[Evidence IEA]; GO_process: GO:1900832 - D-leucine
catabolic process [Evidence IMP] [PMID 10918062];
GO_process: GO:1900829 - D-tyrosine catabolic process
[Evidence IMP] [PMID 10918062]; GO_process: GO:0006399 -
tRNA metabolic process [Evidence IMP] [PMID 10918062]"
/codon_start=1
/product="Dtd1p"
/protein_id="XP_018733467.1"
/db_xref="GeneID:30035843"
/translation="MPNFQEKVLTNKSVSQFTLLAKTKKGAKPDFHAAAKGDHARTLY
NKVLEEVAKGLPGGQSRVGDGVFGAMMDVALVNDGPVTIQLDSKETKSGNILQPSPTP
PQ"
gene <262458..>265166
/gene="pof10"
/locus_tag="AWJ20_3785"
/db_xref="GeneID:30035844"
mRNA <262458..>265166
/gene="pof10"
/locus_tag="AWJ20_3785"
/product="F-box protein Pof10"
/transcript_id="XM_018880815.1"
/db_xref="GeneID:30035844"
CDS 262458..265166
/gene="pof10"
/locus_tag="AWJ20_3785"
/codon_start=1
/product="F-box protein Pof10"
/protein_id="XP_018733468.1"
/db_xref="GeneID:30035844"
/translation="MHSQASGSTDTPKTSEIATESATVSADTTATSSQHVSQEYEPEA
VESATADSSFDNSNITSDGSSAEDEATDLDQITDNAIKISGYERHLDQDVDEVIPSHR
RSNVTSNLPFQSLSSDRKLKGKTSNNPFAVPPSVAENLPNEILVSVFSYLSPGCLDAA
SLVCRKWHQAASEASWRASFLRHFSSAKFNRVTSSLKWRTELMTRLDYLHKWKKGHCN
NLSFNALIWNISHVFCDFEASRITAFSIWRGIGTIADPTRGKVANQRVFTNKMLQAVA
DESCVDGSRFGLIYGFFSDGRVQATLFSHETRLRDYVTMKGPRHFGDVSAVWINKLES
PRTTNKVAALSAGEDGHVFQWEFNTCKLVRDFAVCPQYTDGEDEEGKPKIVKSRRIIH
IQCDSKDKIVCINSHGEVFASVGGSEFVLIGCFIHPVVQDDTSAVSVVSYFDVDFSSN
YVVYAHGQYAMRFKLTLDKSKFESVHFSLGGMSPHGYITAISMDHTTFPQSDLSTDTP
GLNFRYVAAATSDNLVFVWLLQDNADENGSIAPLRRSESPFQVSEESQIGGVLPNEIP
EFRPSISAIALNSMVLLLGSYNGVTVALDLLTGEFLRVVSARFSKRALNLRATEDPNS
VSLWPITHLEIDPDPANPHGIIVVRSAIQYFDLGADLRKSTIKKKGVQKKRNARVPYE
GQGLSRARLSEDIEMDLEMMELEDEEIDEARQHRQHLDGYLAHDLSEEDQLKYAMLLS
EEAAKKESQPKTEEEEDEEMRRAIDLSIAESGAAASLPSSSQTNEKASSSKHLDSPPP
SYDDMDEDLKLAIELSRAEAGTSDAESLYAVPNYINRGESSTHNQSDQEQLDLELKLA
IEMSKEDSGYISDSQLYDPPSAGANPDYDSDLELAMRLSLSEANKS"
gene complement(<265219..>267531)
/locus_tag="AWJ20_3786"
/db_xref="GeneID:30035845"
mRNA complement(<265219..>267531)
/locus_tag="AWJ20_3786"
/product="hypothetical protein"
/transcript_id="XM_018880816.1"
/db_xref="GeneID:30035845"
CDS complement(265219..267531)
/locus_tag="AWJ20_3786"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733469.1"
/db_xref="GeneID:30035845"
/translation="MLSASISRDFTTASLVCNLGYTLQSMACGFFVNAPTMPVYVRWT
RWIAYVYYAFGTVVSNQFNGFLGDCPYDLADDRCDPYTGEYILQTLGFPKNWILVPMM
VNIGWAVGFHILAYLLLKYVRVSVAVSKTFTVKKIEEVAAVPSTRSDSARSEITVTLN
DIRLSVIRRQRLGLTTREIEILRGISATFVPGSINAILGPSGSGKSSLLNFMADRLHS
SFNQKYMSTGDIIFNDKLPSRSLVRSLCSYVTQEDDGLLPSLTVRETLYYAADLRLPK
TMSKQEKRARANEIIMKMGLKDCADTLIGGEFVKGISGGEKRRVTISIQLLNDPKILL
LDEPTSGLDSFIASSILQVLKALAEEGRTIICTIHQPRSDLFAQFGNILLLAKGGRVA
YNGQSTQMLDYFASLNHPCPPLTNPADFVLDLVSVNLQSEEKESISRLKVTKLLDHWQ
NVEDPALERPLVDAHLGLAAAEAEFSSQVREPSKFLPAYQTLLRRSTLNFSRSPHMIV
ARIMQVAGIGIIFALFFSPLKDDMIGVTNRLGLVQEITALYFVGMLNNMAIYPYDRSV
FYREHDDNVYGVLPFFLVYLTLELPFECITSLIFAVFLVLVPGLPRTPELFFACAYCA
LAIVNCGESIGIAFNTLFLHEGFAVNLISVVLSIGCLMAGIMSLEMPGFLKGVNWLSP
LKYAVSVMINMSFDGQTFNCVGQAIDADGGCEFDSGAKVLENYGLKANVPAYLGGIAV
AAVVYRVLALVVLKINRLKLGIKTLSRNSR"
gene complement(<267669..>269339)
/locus_tag="AWJ20_3787"
/db_xref="GeneID:30035846"
mRNA complement(<267669..>269339)
/locus_tag="AWJ20_3787"
/product="hypothetical protein"
/transcript_id="XM_018880817.1"
/db_xref="GeneID:30035846"
CDS complement(267669..269339)
/locus_tag="AWJ20_3787"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733470.1"
/db_xref="GeneID:30035846"
/translation="MSEASSSQYLDDIGQLTYEESTLSPIDAGFSPVGSKGQEKEVEQ
NIEIDSSPLSTVQSTGLTLSSIDKVTVNVRNLSLTVAPTDPASLLGRLLKVKSALGGG
SKSEEDAESSTGTSLVETWHDNEKTIDSNAPDVLSGSRKKILQNISVDVPAGSMMAII
GGSGSGKTSLLNVMAYRMGSRNLHCEGSVTFNGNPNINHVRNAYVMQQDVLQPNLTCR
ETLVYAANLRLPSSTSRQQRADLVEEIILELGLKECANTLVGDTAHKGLSGGEKRRLS
IGIQLLANPSVLFLDEPTTGLDANSAFLLVETCKKLVLKGRTIIMSIHQPRSDIFFLF
DSVTILTRGQAVYSGSVRDVLPYIEQLGYNFPTHMNPADFLIDLSAIDTRTAEQEVKT
KRVVNRLLAAWIANQRFGPVMQPDSVPPSSSAALAKAPLWREIAVLTKRSFIVSMRDP
MGLVGLLLECVLMGTICGWIFFKLDGSISGIRSMEGLLYTSCAAQGYLILLYETYRLC
GPDLRVFDREYNDGSVGPFGYLISRRLSKMFTEDLYVPLIYSVSNILF"
gene <274120..>275625
/gene="GPR1"
/locus_tag="AWJ20_3788"
/db_xref="GeneID:30035847"
mRNA <274120..>275625
/gene="GPR1"
/locus_tag="AWJ20_3788"
/product="Gpr1p"
/transcript_id="XM_018880818.1"
/db_xref="GeneID:30035847"
CDS 274120..275625
/gene="GPR1"
/locus_tag="AWJ20_3788"
/inference="similar to AA sequence:KEGG_Orthology:K12762"
/note="Plasma membrane G protein coupled receptor (GPCR);
interacts with the heterotrimeric G protein alpha subunit,
Gpa2p, and with Plc1p; sensor that integrates nutritional
signals with the modulation of cell fate via PKA and cAMP
synthesis; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 10655215]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 9388468];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9524122]; GO_function: GO:0004930 - G-protein
coupled receptor activity [Evidence IEA]; GO_function:
GO:0004930 - G-protein coupled receptor activity [Evidence
IPI] [PMID 9388468]; GO_function: GO:0004930 - G-protein
coupled receptor activity [Evidence IMP,IPI,ISS] [PMID
9524122]; GO_function: GO:0005536 - glucose binding
[Evidence IMP] [PMID 15494315]; GO_function: GO:0004871 -
signal transducer activity [Evidence IEA]; GO_process:
GO:0007186 - G-protein coupled receptor signaling pathway
[Evidence IEA]; GO_process: GO:0007186 - G-protein coupled
receptor signaling pathway [Evidence IPI] [PMID 9388468];
GO_process: GO:0007186 - G-protein coupled receptor
signaling pathway [Evidence IMP,IPI] [PMID 9524122];
GO_process: GO:0051594 - detection of glucose [Evidence
IMP] [PMID 15494315]; GO_process: GO:0009731 - detection
of sucrose stimulus [Evidence IMP] [PMID 15494315];
GO_process: GO:0010255 - glucose mediated signaling
pathway [Evidence IGI,IMP] [PMID 10361302]; GO_process:
GO:0010255 - glucose mediated signaling pathway [Evidence
IMP] [PMID 15494315]; GO_process: GO:0010255 - glucose
mediated signaling pathway [Evidence IMP] [PMID 9813141];
GO_process: GO:0009757 - hexose mediated signaling
[Evidence IMP] [PMID 10655215]; GO_process: GO:0009757 -
hexose mediated signaling [Evidence IGI,IMP] [PMID
9813141]; GO_process: GO:0001403 - invasive growth in
response to glucose limitation [Evidence IMP] [PMID
12150916]; GO_process: GO:0007124 - pseudohyphal growth
[Evidence IMP] [PMID 10655215]; GO_process: GO:0001302 -
replicative cell aging [Evidence IMP] [PMID 15722108];
GO_process: GO:0007165 - signal transduction [Evidence
IEA]; GO_process: GO:0009745 - sucrose mediated signaling
[Evidence IMP] [PMID 15494315]"
/codon_start=1
/product="Gpr1p"
/protein_id="XP_018733471.1"
/db_xref="GeneID:30035847"
/translation="MSDYYDSDTSKVNNYSAYQALVLRWVSVSSSCLSIVFGLAALYF
YTNMKTKVFRHHLILLLLLFDFGKAVVLLWYPARVLLVASAYDNINFCDVVGFFTSAF
IEGADLAVLALAIHTALLIFKKYKGPEGGLYRYRYWVYALTIIIPLIMAALVFVEGRK
SYRPYITWCYLPVRPLWYRLVLSWIPRYIIIVTIVSIYVAIYIYVKLQYREVVRNFSQ
SQSYVDKYDAKLVGKGIRPRFRRGWRMFKAGTLSFLSYFPGFAFLATSNENITDSPSQ
PIDVQANTIAEFQRASMADFQFRRSVIERQIRSIFVYPTAYVFLWLAPFALQCLQFRS
DLTKSTVFWVNAIAAFMQPFNCVVDTIAFFIRETPWRNREERVFKKETGRKILRGLSI
RRTDGCKWNSSRSPSSPSSDKPSRNTQESDLYTPDIKGGYDVVELQTRGRQYSNDDIM
TGGSSSTNYSPKHTVYPPFPGNELVRSDNLDAESDADIDIFEFLKGPPPHP"
gene complement(<275748..>276599)
/gene="RKM5"
/locus_tag="AWJ20_3789"
/db_xref="GeneID:30035848"
mRNA complement(<275748..>276599)
/gene="RKM5"
/locus_tag="AWJ20_3789"
/product="Rkm5p"
/transcript_id="XM_018880819.1"
/db_xref="GeneID:30035848"
CDS complement(275748..276599)
/gene="RKM5"
/locus_tag="AWJ20_3789"
/note="Protein lysine methyltransferase; monomethylates
Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member
of the seven beta-strand methyltransferase superfamily;
orthologs only found among fungal species; GO_component:
GO:0005575 - cellular_component [Evidence ND];
GO_function: GO:0008757 - S-adenosylmethionine-dependent
methyltransferase activity [Evidence ISM] [PMID 19351663];
GO_function: GO:0008757 - S-adenosylmethionine-dependent
methyltransferase activity [Evidence IDA] [PMID 21460220];
GO_function: GO:0008168 - methyltransferase activity
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0032259 -
methylation [Evidence IEA]; GO_process: GO:0006479 -
protein methylation [Evidence IDA] [PMID 21460220]"
/codon_start=1
/product="Rkm5p"
/protein_id="XP_018733472.1"
/db_xref="GeneID:30035848"
/translation="MLTQDLEPVDEDSLYDHVYELIVSRAGSSQSELGLGYVSRTDLV
EISVGDQELEIKQSLSMLNSSQESSTTGAVIWKVSPLFADWLLTETSLFNKTIVNSSQ
TVVELGTGVAGIVAATLGQKVGRYIATDQEHILKLLSYNIENNTASSKISSKKRVKAA
AVPSQISVCEYDWEHLETFGNFKDGIEGLGSNGTIIACDTIYNDYLIPHFVNALLTVA
KTMGPEHNIIIAQQLRTHEILEASLSQLLEAGFNVWSVPDELLSKKLRNGFAVHYLQL
PLEDKSF"
gene complement(<276882..>277388)
/gene="HOM2"
/locus_tag="AWJ20_3790"
/db_xref="GeneID:30035850"
mRNA complement(<276882..>277388)
/gene="HOM2"
/locus_tag="AWJ20_3790"
/product="aspartate-semialdehyde dehydrogenase"
/transcript_id="XM_018880821.1"
/db_xref="GeneID:30035850"
CDS complement(276882..277388)
/gene="HOM2"
/locus_tag="AWJ20_3790"
/inference="similar to AA sequence:KEGG_Orthology:K00133"
/note="Aspartic beta semi-aldehyde dehydrogenase;
catalyzes the second step in the common pathway for
methionine and threonine biosynthesis; expression
regulated by Gcn4p and the general control of amino acid
synthesis; GO_component: GO:0005737 - cytoplasm [Evidence
IEA]; GO_component: GO:0005737 - cytoplasm [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 16622836];
GO_function: GO:0003942 -
N-acetyl-gamma-glutamyl-phosphate reductase activity
[Evidence IEA]; GO_function: GO:0051287 - NAD binding
[Evidence IEA]; GO_function: GO:0050661 - NADP binding
[Evidence IEA]; GO_function: GO:0004073 -
aspartate-semialdehyde dehydrogenase activity [Evidence
IEA,IEA]; GO_function: GO:0004073 - aspartate-semialdehyde
dehydrogenase activity [Evidence IDA] [PMID 4145650];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA]; GO_function: GO:0016620 - oxidoreductase
activity, acting on the aldehyde or oxo group of donors,
NAD or NADP as acceptor [Evidence IEA]; GO_function:
GO:0046983 - protein dimerization activity [Evidence IEA];
GO_process: GO:0006531 - aspartate metabolic process
[Evidence IEA]; GO_process: GO:0008652 - cellular amino
acid biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0006520 - cellular amino acid metabolic process
[Evidence IEA]; GO_process: GO:0009090 - homoserine
biosynthetic process [Evidence IMP] [PMID 4380684];
GO_process: GO:0009097 - isoleucine biosynthetic process
[Evidence IEA]; GO_process: GO:0009085 - lysine
biosynthetic process [Evidence IEA]; GO_process:
GO:0009089 - lysine biosynthetic process via
diaminopimelate [Evidence IEA,IEA]; GO_process: GO:0009086
- methionine biosynthetic process [Evidence IEA,IEA];
GO_process: GO:0009086 - methionine biosynthetic process
[Evidence IMP] [PMID 4380684]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0009088 - threonine biosynthetic process
[Evidence IEA,IEA,IEA]; GO_process: GO:0009088 - threonine
biosynthetic process [Evidence IMP] [PMID 4380684]"
/codon_start=1
/product="aspartate-semialdehyde dehydrogenase"
/protein_id="XP_018733473.1"
/db_xref="GeneID:30035850"
/translation="MDILDNIIPYISGEEDKLETEPRKILGAVSSDKVSFSIIPENEM
KISATTTRVPVTDGHTACVSIKFAKQPAPSIAEIEKVLSEYTCEAQQLGCHSAPAHAI
DVLSQPNRPQPRLDRDRGNGYTVSVGRIRPDPVLDVKFVALSHNTVLGAAGSGILIAE
LLLAKNLL"
gene <278542..>281805
/gene="HOS4"
/locus_tag="AWJ20_3791"
/db_xref="GeneID:30035851"
mRNA <278542..>281805
/gene="HOS4"
/locus_tag="AWJ20_3791"
/product="Hos4p"
/transcript_id="XM_018880822.1"
/db_xref="GeneID:30035851"
CDS 278542..281805
/gene="HOS4"
/locus_tag="AWJ20_3791"
/note="Subunit of the Set3 complex; complex is a
meiotic-specific repressor of sporulation specific genes
that contains deacetylase activity; potential Cdc28p
substrate; GO_component: GO:0034967 - Set3 complex
[Evidence IDA] [PMID 11711434]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0016575 -
histone deacetylation [Evidence IDA] [PMID 11711434];
GO_process: GO:0045835 - negative regulation of meiosis
[Evidence IPI] [PMID 11711434]"
/codon_start=1
/product="Hos4p"
/protein_id="XP_018733474.1"
/db_xref="GeneID:30035851"
/translation="MASDNVASSDTGPVNDEGSTGSGNGNSTSPSGGGSNSGPPSSGW
RFSASTASTKPGAVINKRTLAEYNSSLSTKNQATQAKQQTNQENLTEQKSESNKGHRE
TRENYDRKVGDDMENDDSTAETDDAHEADSSEAETVILEESKEPKRRNLSVVESGNGS
NDSGVKIKIRKLSDASEGRSAHSKSNKPLKRLQKRIHVSDDDDDDEYSGDKDDSENEN
DNDDHEHRNEQRHNRQPFQRAGGASGTLSDSDDWNDPRGRVKSRSRDTSRNSSSSTAA
NANRKVKSVVTAKMRKDGRSHVKDTDDGKHSRSVSPAVRSGNHNVTASGLTMPVQPIR
KDGSPIRDASGRLLLQKLCDKGDFERSKALIEMGVDVNDRDYAGNTALHDAALKGHLN
IVELLLDNGAIIDIRSGISDLDTPLIDATSRGHVEVVKLLLSRGADPRIFNAQGKTAL
DFLSQDDEHFNTLESLLKEYSIKMRNKPRRGSDTGGAEDENNGGNGSAANNSAYDAAL
HATPGTFPDAYFEGRRGGGNYSDGGYSSSGSQSGHRQKHSHNNQVSHGHNNHHHHHNR
SHSPSHTHNHGTGHHTTSGDADESSSLSHDEGSKSSSSHTRRRGGARSQSIRNDLLWM
DLTTKTGREQVYRRAADGDLQYVGRSLEEGWQPDADCLVLAAKHGHTEVVGLLLAFGV
SSDQLNDEGVTALHETVGRGHMDTIRLLLDSGADPCWVDAQGRSYLDLAKEALGSDDE
ETILIRDAVDKKKKIPGKKDQTSSSSSSSASNLIKSKSDVEKRRKVSDDHEIPPDRIK
KRKLQKVEKIDKAEKAEKAEKSEKAKSRMESPARVKEEPSSDVRSRQLDEKAKAEAAE
SAARELRKQQEAAAEKKAREEAALAEKKRLEEIERERLKELERKRAEEAEIKRAEEAE
KARQAREAERRAEEEKRRIQKEKERELARKREIIAAQKERERKEREFQMLKSLEEEEN
RRKEKIKREQELEAERLRQLQIEEERKKALEAEQELDRQRMLQLQQEAEARKNFPYAI
RMATYGPKQLDEMMSFFPILVHSVVPDTSYFLDIQVSLLLGLPNVHQSCKLYVAVQT"
gene complement(<282424..>283662)
/locus_tag="AWJ20_3792"
/db_xref="GeneID:30035852"
mRNA complement(<282424..>283662)
/locus_tag="AWJ20_3792"
/product="hypothetical protein"
/transcript_id="XM_018880823.1"
/db_xref="GeneID:30035852"
CDS complement(282424..283662)
/locus_tag="AWJ20_3792"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733475.1"
/db_xref="GeneID:30035852"
/translation="MANLQNSSSLESSMSGSFIVSSTASVATETYQRAAKLFLTKSFV
DSLNTLEPLLADAKELRRTGQLSEKTWIKSWKLYFVLLDAAARQQEEASTRSPSSSSS
SSSSSISGTNSVSSSWPKKVRLELVKKITDGSLWKQVENEFGGSLSSAPPDIVFGLTS
LTIRHGTTNNSILSMVSESLELYLSSMSDLVQSFDDTYRHEYLKVLDLYILKVLPLRN
EYDLAREMIESSQFYDSHVKEEKRRQLIKSEENLVKKKQKKEEEDSKRKQMEALREEA
KRKHERLVQQQRQQQKQLQQQQQQQQQLEQKTSLEHQQSSVNSLSSSGLGPRRTASPT
PPRSSNPVRSFDSLIFYWRRRATAIVQSAMVWKIIAFFIIIVLSVSNPLARERARRIV
NNVWRKLSETARMGFKVSFV"
gene <287753..>288880
/gene="FBP26"
/locus_tag="AWJ20_3793"
/db_xref="GeneID:30035853"
mRNA <287753..>288880
/gene="FBP26"
/locus_tag="AWJ20_3793"
/product="Fbp26p"
/transcript_id="XM_018880824.1"
/db_xref="GeneID:30035853"
CDS 287753..288880
/gene="FBP26"
/locus_tag="AWJ20_3793"
/inference="similar to AA sequence:KEGG_Orthology:K19029"
/note="Fructose-2,6-bisphosphatase, required for glucose
metabolism; protein abundance increases in response to DNA
replication stress; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_function: GO:0003873 -
6-phosphofructo-2-kinase activity [Evidence IKR] [PMID
9032445]; GO_function: GO:0003873 -
6-phosphofructo-2-kinase activity [Evidence IKR] [PMID
9032446]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0004331 -
fructose-2,6-bisphosphate 2-phosphatase activity [Evidence
IEA,IEA]; GO_function: GO:0004331 -
fructose-2,6-bisphosphate 2-phosphatase activity [Evidence
IMP] [PMID 1322693]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0016311
- dephosphorylation [Evidence IEA,IEA]; GO_process:
GO:0006003 - fructose 2,6-bisphosphate metabolic process
[Evidence IEA]; GO_process: GO:0006000 - fructose
metabolic process [Evidence IEA]; GO_process: GO:0006006 -
glucose metabolic process [Evidence IMP] [PMID 1322693];
GO_process: GO:0008152 - metabolic process [Evidence IEA]"
/codon_start=1
/product="Fbp26p"
/protein_id="XP_018733476.1"
/db_xref="GeneID:30035853"
/translation="MEAAQPSALFFDAHNEEGARLRRQAAIRAIEDMLKWFSEEDGVV
GILDATNSTKQRRQWIQDLLVKQGIEPMFVESWCDDQELIMHNIMDVKTCSPDYTGQD
PDEAAKDFMERIKKYEEVYETINDESLTYAKIINVNSQVIINRIQSYVQSRIVYYLMN
LHIKRRCIYLSRHGESEFNLTGQLGGDSNLSPRGDLYAHKLPQLVKEAVGDQPLTVWT
STLKRTQQTARFLPYRQMQWKALDELDAGVCDGMTYEEIKQEYPEDFEARDANKFEYR
YRGGESYRDVVARLEPIIMELERQENIMIVTHQAVLRCIYAYFMNVPQDRSPWMDVPL
HTLIRLEPRPYSTLETRVPANIPAVSTYREKGSTVLAAPTK"
gene <289346..>291289
/gene="SRS2"
/locus_tag="AWJ20_3794"
/db_xref="GeneID:30035854"
mRNA <289346..>291289
/gene="SRS2"
/locus_tag="AWJ20_3794"
/product="DNA helicase SRS2"
/transcript_id="XM_018880825.1"
/db_xref="GeneID:30035854"
CDS 289346..291289
/gene="SRS2"
/locus_tag="AWJ20_3794"
/inference="similar to AA sequence:KEGG_Orthology:K03657"
/codon_start=1
/product="DNA helicase SRS2"
/protein_id="XP_018733477.1"
/db_xref="GeneID:30035854"
/translation="MSEQDPGDASLAGGAIGYDLISGQALESKAWGIEEDLNDQQLRA
VQLPVDANIQVIAGPGTGKTKTLVNRLLYILTEYNVPPEKVLVLTFTNRAIHIFRESL
FKLAPAIAPQVEIHTFHSYCQSLISDHWEEVGLRPGWTVADDMDQEYILTEVIKETVG
SHTTAREFRVLSNKLHTYKRSLLNKSAKRDSKLDEILTKYETRLAGCNMLDYDGILKL
GYRLAEKYSKAPESDKNKYFSALLIDEFQDANLFQWDLTCLIAKGSASITIVGDPDQS
IFSFQGASSNLLDIMKKQLSDVRTVVLTQNYRSSQEIVDAASAMIREDSHFLGDEQMP
VGLFTGPKPIFKKSLDSTSETVWVANTIHHLTHKENVEPSSIAVLVRTNGRKDSVAAG
LRQRGIEVAIYGPLSLLKSPYIRPLYSFLKFIQNFEQDIQLLYMLRNPNKILSPKQIS
EAQSFSRSRGLPLWEALQQNQLWLRQPTTRVNKSKKPHGPPDLDQFISVINKSADIIH
SNVHDSESLVEGLQLVSQYLSWPTRYGDKAHVKQQELFDLITSIAPLLKDESTSLSSS
LLAHTRLQDIAAKPGEVVVSTIHNAKGRLQHCLRRLGLRPRPRGSCFAGECWERRRND
SSAARGAAGSGAEPQPPEAPYRM"
gene complement(<291885..>292643)
/locus_tag="AWJ20_3795"
/db_xref="GeneID:30035855"
mRNA complement(<291885..>292643)
/locus_tag="AWJ20_3795"
/product="hypothetical protein"
/transcript_id="XM_018880826.1"
/db_xref="GeneID:30035855"
CDS complement(291885..292643)
/locus_tag="AWJ20_3795"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733478.1"
/db_xref="GeneID:30035855"
/translation="MSKVYFISGGNRGIGFGFVQELSQRKDVTVIASVRNAAKATELN
AWIKEHPNIHTVNYDGNVDGSALEAAKEVESITGGKGIDVFIANAAISDAHYSVLDLP
EKNWLEHYKINTLAPLLLFKAFYPLLKKKDTRQVIFISSDAGSIAATPKMSFSAYGQS
KAALNYSTKELSFELEEEKFTIIALNPGPVLSDMGTFSREEMKKISVAFSHKHWDTAP
SVKESVDELLLVIDKLTPADNGKFFHHDGTIYPW"
gene complement(<293054..>294529)
/gene="STL1"
/locus_tag="AWJ20_3796"
/db_xref="GeneID:30035856"
mRNA complement(<293054..>294529)
/gene="STL1"
/locus_tag="AWJ20_3796"
/product="glucose-inactivated glycerol proton symporter
STL1"
/transcript_id="XM_018880827.1"
/db_xref="GeneID:30035856"
CDS complement(293054..294529)
/gene="STL1"
/locus_tag="AWJ20_3796"
/codon_start=1
/product="glucose-inactivated glycerol proton symporter
STL1"
/protein_id="XP_018733479.1"
/db_xref="GeneID:30035856"
/translation="MFAIPWFDSNPEGFFKLKGSRLIWATAILSSLGFSMIGYGNALM
GGIINEEPFKQRFDYPSSSEIGFITAIYGVGAFVGSILTSLVGDSFGRRKSIAIGAIV
MILGSLVQSSAITLRSLSVGRFVSGTGLGFLNSTIPVLQNEVSPHGERGMYVAVYCTT
LNVGILVAYWVNFSLRNLGSDKSWRLPILLQIFILAPILALTVLTCESPRWLLLKDRD
SNALQVLANLENSEPDDPLVEDKFHSLKESVVWDTVDAEQSSFWQDFYNPNDIIATKR
LWISLSVQIFQQLGGINVLIYYCPILFEDSIKLSSQTSSQLSVLLQIWLVVTSMVPWS
LLDRVGRRPLILGGISGQGASMLMQAIFIYQLNIHGNSNDSEKSSLSIWAILSIVTLF
TFEASFAIGMQASVWVYCSEILPLRLRGRGTSLATSANWATNSVLVHLAPVVLEKLNY
ASFLILAALNFTFLPVAYLAFCETKGLSLEQVDQLFLEHQD"
gene <296215..>298728
/gene="MPS1"
/locus_tag="AWJ20_3797"
/db_xref="GeneID:30035857"
mRNA <296215..>298728
/gene="MPS1"
/locus_tag="AWJ20_3797"
/product="serine/threonine/tyrosine protein kinase MPS1"
/transcript_id="XM_018880828.1"
/db_xref="GeneID:30035857"
CDS 296215..298728
/gene="MPS1"
/locus_tag="AWJ20_3797"
/inference="similar to AA sequence:KEGG_Orthology:K08866"
/codon_start=1
/product="serine/threonine/tyrosine protein kinase MPS1"
/protein_id="XP_018733480.1"
/db_xref="GeneID:30035857"
/translation="MSVNDGEHHEGDDSVSSSDGSFMPPVLSDYANQLLFEDKERQSR
LQYLQQQESYQDSRGANVGYQQSHHYNQGSVGLPNGDYSPVDKMVISPQVPKTSRREL
SGSPHMNSSGGISSSSLGTSPLESSDKISNASTTPGIRPAATLFTRAPAADMTSLRTT
TMRSKRFGSNAFNKRLGKPLNFKGLPSANPNTTSNSTSTAISSMAQLSVSSSTATSSN
PASNTASMHQPLPATTTSFSAGTSSMSIPATSLATTTATRPANIYSSLSGVDEDRNPN
LSPSTKRSTTRIRFAEEHQEIAHDSPRRSYLLRHSKSSDNNSQYHDSDIGEANSSVSS
NSSKRDRRDWSSRATTPVGDTSRHSSSSKSDHSQYDANPQQGSLSQFRAQDERPVLQS
ISPNIPVNPGNRFGSKVPPPKLENWANVKTSSLFSSNELHEYHNNHHNHHEVQEPERP
APPVPTDRADRAARIERFEKAAEQQSVAQLHAAHNLGTDRPRQSSDRSKTKKNMMAIN
NRLYQRLELLGRGGSSKVYKVQSNEGKVFAVKKVTFEDVDESVISGFKGEIDLLQRLR
NEDRVVKLVDYEMHDTSVYLVMECGEIDLAHVLSARLNSQLDISFVRFYATEMLRCVA
AVHNQGIVHSDLKPANFLLVKGMLKIIDFGIANVVPDYTANIHRDTQIGTPNYMAPEA
LLDANQFGPGSGTETAAKFKVGKPSDVWSCGCIIYQMVYGKPPYAAYSGAQRMLAIMN
PKVQIQYPKHGLGGVRVSRDAIETIRGCLHRDPAARMTVEEVLEGSFLNPQAVDRSFI
RTLLEHAVQYGATRKEPILPREVDMLTDDIWKKIQLSNR"
gene <302689..>303480
/gene="RAS2"
/locus_tag="AWJ20_3798"
/db_xref="GeneID:30035858"
mRNA <302689..>303480
/gene="RAS2"
/locus_tag="AWJ20_3798"
/product="Ras family GTPase RAS2"
/transcript_id="XM_018880829.1"
/db_xref="GeneID:30035858"
CDS 302689..303480
/gene="RAS2"
/locus_tag="AWJ20_3798"
/inference="similar to AA sequence:KEGG_Orthology:K07827"
/note="GTP-binding protein; regulates nitrogen starvation
response, sporulation, and filamentous growth;
farnesylation and palmitoylation required for activity and
localization to plasma membrane; homolog of mammalian Ras
proto-oncogenes; RAS2 has a paralog, RAS1, that arose from
the whole genome duplication; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IDA] [PMID
22575457]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 22575457]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 23127800]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 11914276]; GO_component: GO:0005886 - plasma
membrane [Evidence IDA] [PMID 16622836]; GO_component:
GO:0005886 - plasma membrane [Evidence IDA] [PMID
20162532]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 23127800]; GO_function: GO:0005525 -
GTP binding [Evidence IEA,IEA]; GO_function: GO:0005525 -
GTP binding [Evidence IDA] [PMID 6438624]; GO_function:
GO:0003924 - GTPase activity [Evidence IDA] [PMID
8106517]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_process: GO:0006184 - GTP catabolic
process [Evidence IEA]; GO_process: GO:0007190 -
activation of adenylate cyclase activity [Evidence IDA]
[PMID 8106517]; GO_process: GO:0030437 - ascospore
formation [Evidence IMP] [PMID 2558958]; GO_process:
GO:0045762 - positive regulation of adenylate cyclase
activity [Evidence IGI] [PMID 2981630]; GO_process:
GO:0045762 - positive regulation of adenylate cyclase
activity [Evidence IGI] [PMID 3891097]; GO_process:
GO:0000411 - positive regulation of transcription by
galactose [Evidence IMP] [PMID 16292676]; GO_process:
GO:0097271 - protein localization to bud neck [Evidence
IGI] [PMID 12782684]; GO_process: GO:0007124 -
pseudohyphal growth [Evidence IMP] [PMID 1547504];
GO_process: GO:0032880 - regulation of protein
localization [Evidence IMP] [PMID 15917658]; GO_process:
GO:0001302 - replicative cell aging [Evidence IMP] [PMID
12839995]; GO_process: GO:0001302 - replicative cell aging
[Evidence IMP] [PMID 8034612]; GO_process: GO:0007165 -
signal transduction [Evidence IEA]; GO_process: GO:0007264
- small GTPase mediated signal transduction [Evidence
IEA]"
/codon_start=1
/product="Ras family GTPase RAS2"
/protein_id="XP_018733481.1"
/db_xref="GeneID:30035858"
/translation="MSRRESNPLYKLIVLGDGGVGKTALTIQLCLNHFVETYDPTIED
SYRKQVMIDDQPATLEVLDTAGQEEYTALRDQWIRDGEGFILVYSITSRSSFSRIKNY
YSQIERVKDEDPFAIVLVGNKSDRKGERQVSEQDGEQLARELGDNVAFFESSAKLNIN
IEKAFFECARKCRVIRHPETNPQGGPNSNNNLADVHSGYVDQGLGHGGNALGNQSSQN
AGQNQKAPANQAHNRPSHNAGTSSQSHGRSDTRKAQKKKSKCIVM"
gene complement(<306722..>308389)
/gene="YHK8"
/locus_tag="AWJ20_3799"
/db_xref="GeneID:30035859"
mRNA complement(<306722..>308389)
/gene="YHK8"
/locus_tag="AWJ20_3799"
/product="Yhk8p"
/transcript_id="XM_018880830.1"
/db_xref="GeneID:30035859"
CDS complement(306722..308389)
/gene="YHK8"
/locus_tag="AWJ20_3799"
/note="Presumed antiporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; expression of gene is up-regulated in cells
exhibiting reduced susceptibility to azoles; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISS]
[PMID 9046086]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA,IEA]; GO_function: GO:0015297 -
antiporter activity [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND];
GO_function: GO:0005215 - transporter activity [Evidence
IEA]; GO_process: GO:0015893 - drug transport [Evidence
IEP] [PMID 12697649]; GO_process: GO:0006811 - ion
transport [Evidence IEA]; GO_process: GO:0015992 - proton
transport [Evidence IEA]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA,IEA]"
/codon_start=1
/product="Yhk8p"
/protein_id="XP_018733482.1"
/db_xref="GeneID:30035859"
/translation="MAEPESGIRRSRSNSFSHHESSGPTASSPTLSMNSSNPDLMSKK
VFNEDPEKQRDLKTDDDDVEAAPKPLTDDTTAENPVIPFDSTTPTHDPNCVYWDGLQD
KENARRMSFARKWFICIILTSTAGCLTALSSAWTSAVDPMVERFHSSAEVGDLGLTLF
LFGLGIGPLIAAPLSEKFGRQPVYLISLFLFFVFQFPTAFAHNMASALVGRYLCALGG
SAFMSNVPGTFSDLFEKHQLALPVTVFTFGPFLGPGIGPVISGPITQHVGYRWVFYVF
LIWTFVLLVTIALTVPETYPPILLAKKAKRLRKETGNDAIYAPIEKSERSFWRSLRHS
CSMPVILISHEPVLDILCFYTGFLLAIIYLFFVAFPYVFTKVYHFQVEFVGLSFTGII
VGMALAASTYPFWRHFYEKELAKNGGVANPEMRLPQMCFGAVIIPIGLFMFAWTAYAE
VHWIVPMVASGIFAFGSYFTFNGIITYTVETYHRYAASATAANVFIRCAIAGSAPLFG
VQMLEGMGIHWGISLLGFVAVLLCPSAFIFRIYGKTLRMKSKYAIKV"
gene complement(<311367..>311768)
/gene="ESS1"
/locus_tag="AWJ20_3800"
/db_xref="GeneID:30035862"
mRNA complement(<311367..>311768)
/gene="ESS1"
/locus_tag="AWJ20_3800"
/product="peptidylprolyl isomerase ESS1"
/transcript_id="XM_018880832.1"
/db_xref="GeneID:30035862"
CDS complement(311367..311768)
/gene="ESS1"
/locus_tag="AWJ20_3800"
/inference="similar to AA sequence:KEGG_Orthology:K09578"
/note="Peptidylprolyl-cis/trans-isomerase (PPIase);
specific for phosphorylated serine and threonine residues
N-terminal to proline; regulates phosphorylation of the
RNAP II large subunit (Rpo21p) C-terminal domain (CTD);
associates with phospho-Ser5 form of RNAP II in vivo;
regulates phosphorylation of Ser7 within CTD; present
along entire coding length of genes; represses initiation
of CUTs; required for efficient termination of mRNA
transcription and trimethylation of histone H3;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IC] [PMID
10531363]; GO_component: GO:0005634 - nucleus [Evidence
IC] [PMID 10899126]; GO_component: GO:0005634 - nucleus
[Evidence IC] [PMID 10899127]; GO_component: GO:0005634 -
nucleus [Evidence IC] [PMID 11708797]; GO_component:
GO:0005634 - nucleus [Evidence IC] [PMID 14704159];
GO_function: GO:0000993 - RNA polymerase II core binding
[Evidence IPI] [PMID 10531363]; GO_function: GO:0000993 -
RNA polymerase II core binding [Evidence IPI] [PMID
10899126]; GO_function: GO:0016853 - isomerase activity
[Evidence IEA,IEA]; GO_function: GO:0003755 -
peptidyl-prolyl cis-trans isomerase activity [Evidence
IEA,IEA]; GO_function: GO:0003755 - peptidyl-prolyl
cis-trans isomerase activity [Evidence IDA,IMP] [PMID
15728580]; GO_function: GO:0003755 - peptidyl-prolyl
cis-trans isomerase activity [Evidence IDA] [PMID
9867817]; GO_process: GO:0080182 - histone H3-K4
trimethylation [Evidence IGI,IMP] [PMID 22778132];
GO_process: GO:0031064 - negative regulation of histone
deacetylation [Evidence IMP,IPI] [PMID 10899127];
GO_process: GO:2000059 - negative regulation of protein
ubiquitination involved in ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 19597489];
GO_process: GO:0000122 - negative regulation of
transcription from RNA polymerase II promoter [Evidence
IGI,IMP] [PMID 14704159]; GO_process: GO:0045899 -
positive regulation of RNA polymerase II transcriptional
preinitiation complex assembly [Evidence IGI] [PMID
15166139]; GO_process: GO:2000749 - positive regulation of
chromatin silencing at rDNA [Evidence IMP] [PMID
10899127]; GO_process: GO:0035307 - positive regulation of
protein dephosphorylation [Evidence IDA] [PMID 11904169];
GO_process: GO:0035307 - positive regulation of protein
dephosphorylation [Evidence IMP] [PMID 19854134];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IGI,IPI] [PMID 10899126]; GO_process: GO:0006457 - protein
folding [Evidence IEA]; GO_process: GO:0000413 - protein
peptidyl-prolyl isomerization [Evidence IDA,IMP] [PMID
15728580]; GO_process: GO:1901407 - regulation of
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 22778132]; GO_process: GO:0000083 -
regulation of transcription involved in G1/S transition of
mitotic cell cycle [Evidence IMP] [PMID 11708797];
GO_process: GO:0000117 - regulation of transcription
involved in G2/M transition of mitotic cell cycle
[Evidence IMP] [PMID 11708797]; GO_process: GO:0006369 -
termination of RNA polymerase II transcription [Evidence
IMP] [PMID 14704159]; GO_process: GO:0006369 - termination
of RNA polymerase II transcription [Evidence IGI,IMP]
[PMID 19854134]; GO_process: GO:0006369 - termination of
RNA polymerase II transcription [Evidence IMP] [PMID
22778132]"
/codon_start=1
/product="peptidylprolyl isomerase ESS1"
/protein_id="XP_018733483.1"
/db_xref="GeneID:30035862"
/translation="MAKNYSQNASGAAAAQGASGDSEKIRASHLLVKHKDSRRPSSWK
EANISRTKEEALSIILAHEARIRSGAATLSELAVSESDCSSARRGGDLGYFGRGEMQK
EFEDAAFALKPGELSRVVETSSGLHLIERTG"
gene <313836..>314834
/locus_tag="AWJ20_3801"
/db_xref="GeneID:30035863"
mRNA <313836..>314834
/locus_tag="AWJ20_3801"
/product="hypothetical protein"
/transcript_id="XM_018880833.1"
/db_xref="GeneID:30035863"
CDS 313836..314834
/locus_tag="AWJ20_3801"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733484.1"
/db_xref="GeneID:30035863"
/translation="MAPHLLVYAGTSYDKDQLVKVPVNKGDDASYIELDCPHAVDGVV
RLAIRIKNFKDVRSSKHDPPEQEEIEEAVVVEEGSETDPKASVKDMSRSSSTSSNASN
SSRTSTSSRLKNYLKKKKSRSPSPSPADSPYFEQENHKQDQLSIQFSIRFADPVAGDN
LLFGNDFDEPIRDILPYGFSLGYKMFQWYIDPSVEGDPMADKPYLYGRALTSINAIHE
NDSPDIKWDGYVNGDKFLNPGSAPEKVKQMSSSERMTYFRDKSHRQSFDFVANNLYTF
DFFTPYLTLGDSFGVQLPGFKFDVGKYCGGQPLRYVLKNLLDDSIYLVVVFSLVNE"
gene <315717..>316349
/locus_tag="AWJ20_3802"
/db_xref="GeneID:30035864"
mRNA <315717..>316349
/locus_tag="AWJ20_3802"
/product="hypothetical protein"
/transcript_id="XM_018880834.1"
/db_xref="GeneID:30035864"
CDS 315717..316349
/locus_tag="AWJ20_3802"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733485.1"
/db_xref="GeneID:30035864"
/translation="MEIREREVEALKDRESAARKEATALSDGVVVWKDTCETVSDLER
RLSLLPKQDRSDVKKISTMLNETLARLTRNLDVSEDNKWSLLSVAISQEVEALKQGIE
LVESMSAPPSSTSSSKVNSPPTAVSRILPKISHQVPNTALSSLEEPSKSHIIVPTPKT
SPSPSFTNAVTGLNINNVSAEPISSSPTQSSTNAFNSVAQLLGNYETKTD"
gene complement(<316646..>319810)
/locus_tag="AWJ20_3803"
/db_xref="GeneID:30035865"
mRNA complement(<316646..>319810)
/locus_tag="AWJ20_3803"
/product="hypothetical protein"
/transcript_id="XM_018880835.1"
/db_xref="GeneID:30035865"
CDS complement(316646..319810)
/locus_tag="AWJ20_3803"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733486.1"
/db_xref="GeneID:30035865"
/translation="MSDSNPPPLPAGAGDTSSAPVDIEAQLKALLAGLGQDADGDKNA
DNNTNFSTGEHESEEQSEDAATEAARKLIENALYSESSNLEAGVHPTADEQRETEPVE
EPQQVDPVSDSTRPSASADMESLLLEQTLAEVQKALADDQGLSGQDSESQPETNVRST
QDIESSKTVQDSHIAIADQEPDTKENDEQNTDVDQGSLNALIQNALTALDDQPERERQ
DNQDDVPQAPKPLHEEPTVLEHPSSKEQTQHDTFSSDSGASRPSGEPDQAALASAIAN
ALAGVGTRADDTDGSVTETSLQDQIAAAVASVSQEILPVHEDTNQHDTAAITEALVSS
LSGEGANHNQEPDVLHIQQELQRILANDVSQQVSADTESHPQQASQTEANEDGEPEFD
ADAAAASVAEALAKALQQGAGDIDGESGNASAEETELAAQKALMEQLSSALREESAGH
IQGQTGDEQHSVEGVDVQAMAELLSGSDLESTLSSILSGMNDPPQQEEPPRSNLSIAE
TLAISRSNIEKSREKLAKRIDPMLNRRGYLQSWSVRRQQHQQQQQRQHQEQPRQTIQP
QQDADIDPLLQFSSETLPSQEEQSSDQIESGDGAEVPADSMLAALLLAKQMIGDEEAT
SEGQDLSLNEDAMEAVQQALQALSKSIQEEGASGTTTENNLTSDSTTAGNTPIFSSLD
QFRTTRKYKPRRYRANAVLTPEDKERIRMDNRERKKRWRSNNNIRNKDNDLRARLHRR
ATNMFGPEDSPEKKSWFDKEFQKRREKRMLREMQLDPDFQRDDENSNGFVNINSNNNA
NGNSSSAESLRAALEILGKTEGLNEGLAQTVDSLVKDPQLLKTLMGDLFGNEESNNVS
NNTSTATNYTFQPHANVNKPIAGGGIMSTFKIRKTESQPRAVQSRPPTAQPQFPSKYP
DRGLTTLVRRAMKSRGRNTGGFQQPSQSTQPSVPRVLTNVPPLPNMSAPDPVAAIAGA
LSMRADNFSDVQQLSQPPQSLKRKPSLETISVTVSPKPPTYFGKPASRPSSVLQPAKK
FKTMDNRAKAFGFPPLLSAMSTKR"
gene complement(<320293..>320403)
/locus_tag="AWJ20_3804"
/db_xref="GeneID:30035866"
tRNA complement(<320293..>320403)
/locus_tag="AWJ20_3804"
/product="tRNA-Leu"
/db_xref="GeneID:30035866"
gene <321912..>323276
/locus_tag="AWJ20_3805"
/db_xref="GeneID:30035867"
mRNA <321912..>323276
/locus_tag="AWJ20_3805"
/product="hypothetical protein"
/transcript_id="XM_018880836.1"
/db_xref="GeneID:30035867"
CDS 321912..323276
/locus_tag="AWJ20_3805"
/inference="similar to AA sequence:KEGG_Orthology:K13099"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733487.1"
/db_xref="GeneID:30035867"
/translation="MVSNSATFDKRNPLQLRDVDEDDEIDPNDQEAALLRQDIEGSRP
KSKRNAVKLDYDSDSSDDGASRRNKVHKQKKKTQKSSENKDGDSGSDDDMFSDNDNDD
LKEADAIDSDTERRESKKKAKFLDMDKFEKQEELEDLPENSDSDPDDSGSDVNEANVD
IDYFVNAGSPTTTDNAVLDSKPNKVKNAKKTEPKMESFHLRTDLEEGNFDQDGNFIRN
AMDTNAHQDAWLDGLSKHDIKVARQAHQYREKHNVEHGGVDDEESTKFVPKCDVIAAL
LSYLQTGESPLDALQRLNSAAPKKRLGRKSASHAPLKGDIKTDAKESIRIQAIESISE
NSNKLLQEFGLTDIYDKTREELLRQYQKETGEVYVDLKRKRSESSLDGDDDTNDRGVD
GTSTQSSLWEFRWDESDEIHGPYTSEQMASWVEYNYFDERVSVRQVSQGPLDFVPYDQ
VTFL"
gene <323630..>324391
/locus_tag="AWJ20_3806"
/db_xref="GeneID:30035868"
mRNA <323630..>324391
/locus_tag="AWJ20_3806"
/product="hypothetical protein"
/transcript_id="XM_018880837.1"
/db_xref="GeneID:30035868"
CDS 323630..324391
/locus_tag="AWJ20_3806"
/inference="similar to AA sequence:KEGG_Orthology:K05019"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733488.1"
/db_xref="GeneID:30035868"
/translation="MPFAIQTIQSAPLDSEFTPLQEHQSYTPASFSLEENPILYLKST
AAKIETSKPLPQVPNATVITGDIYISSSYLTIWIPDHSVGVTIPYQCITLHAIQSGGE
SVYLQIESEGLVEGNPSFDDAPIMELTISLEKENDPTIRDLYNALSECSSLHLDVSEG
DEEDIDGFEGMGEGDDGWITAESLISGQGDDDVSLDASKENGHGPAAALEVAVADAES
VRAGQRRGREENEDEEVDAEEESVEAEESKWRRTN"
gene complement(<327407..>329305)
/gene="CMP2"
/locus_tag="AWJ20_3807"
/db_xref="GeneID:30035869"
mRNA complement(<327407..>329305)
/gene="CMP2"
/locus_tag="AWJ20_3807"
/product="calcineurin catalytic subunit A"
/transcript_id="XM_018880838.1"
/db_xref="GeneID:30035869"
CDS complement(327407..329305)
/gene="CMP2"
/locus_tag="AWJ20_3807"
/inference="similar to AA sequence:KEGG_Orthology:K04348"
/note="Calcineurin A; one isoform (the other is Cna1p) of
the catalytic subunit of calcineurin, a
Ca++/calmodulin-regulated protein phosphatase which
regulates Crz1p (a stress-response transcription factor),
the other calcineurin subunit is CNB1; regulates the
function of Aly1p alpha-arrestin; CMP2 has a paralog,
CNA1, that arose from the whole genome duplication;
GO_component: GO:0005955 - calcineurin complex [Evidence
IPI] [PMID 10887154]; GO_component: GO:0005955 -
calcineurin complex [Evidence IDA] [PMID 1321337];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0004723 - calcium-dependent
protein serine/threonine phosphatase activity [Evidence
IDA] [PMID 1321337]; GO_function: GO:0005516 - calmodulin
binding [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA,IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0004721 - phosphoprotein phosphatase
activity [Evidence IEA,IEA]; GO_process: GO:0000754 -
adaptation of signaling pathway by response to pheromone
involved in conjugation with cellular fusion [Evidence
IMP] [PMID 1651503]; GO_process: GO:0006873 - cellular ion
homeostasis [Evidence IMP] [PMID 8798496]; GO_process:
GO:0016311 - dephosphorylation [Evidence IEA,IEA];
GO_process: GO:0008152 - metabolic process [Evidence
IEA,IEA]"
/codon_start=1
/product="calcineurin catalytic subunit A"
/protein_id="XP_018733489.1"
/db_xref="GeneID:30035869"
/translation="MSDERAKKLENAIKAFQEKKPLPKVDFSVYKGDDGKELRTDERV
IKTTPAPATKIPTDDELFLPGTSNPRLPNLAFLKDHLFREGRLHEEQAIYIINSATKI
IMEEPTLLEIDPPVTLCGDIHGQYYDLMKLFEVGGDPRNTKYLFLGDYVDRGYFSIEC
VLYLWALKLHYPKSVFFLRGNHECRHLTDYFTFKLECKHKYSLNIYEACMMSFCTLPL
AAVVNKQFFCVHGGLSPELHTLDDVRKLDRFREPPTHGLMCDLLWADPIENFGEEDNN
RSFIHNNVRGCSYFYTYKAACDFLVKNNLLSIIRAHEAQNAGYRAYLESRNTGFPSVM
TIFSAPNYLDTYNNKAAVVKYENNVLNIRQFKHSPHPYWLPNFMDVFTWSLPFVGEKI
TDMLISILNICTREELEEDTVRYIQEKGNTSKYGGIFGRKSPRSPRSPRSPSSPRSPR
SPTSPRSPKKPIAGQTQQYDSDEDLPVKIPEEVEPEEDYFQDAQSTAMDRDDDDLDQE
DDEDEDDLEVRRDRFKNKILAIGRLSRMFQVLRQESESVSEFKATTGTMLPAGTLMSG
AEGIRKAITTFDEARRVDLINEQLPPTADEKAQENERQRKEALNRAMKEADADVKIRN
LARRLTGE"
gene <330504..>331115
/gene="TSR2"
/locus_tag="AWJ20_3808"
/db_xref="GeneID:30035870"
mRNA <330504..>331115
/gene="TSR2"
/locus_tag="AWJ20_3808"
/product="Tsr2p"
/transcript_id="XM_018880839.1"
/db_xref="GeneID:30035870"
CDS 330504..331115
/gene="TSR2"
/locus_tag="AWJ20_3808"
/inference="similar to AA sequence:KEGG_Orthology:K14800"
/note="Protein with a potential role in pre-rRNA
processing; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0000462 -
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
12837249]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]"
/codon_start=1
/product="Tsr2p"
/protein_id="XP_018733490.1"
/db_xref="GeneID:30035870"
/translation="MSEPLASVQGEGANLVLSDPKHQSRFELGVCMAVYQWDNLQTAV
DNQWGGADSEDKRDWMVGSVVELFETSNYVDSDDIEDRLLGIMEDEFGVSIEDNTATP
VAVSIIQLYKECAEGNFSHVDELYAKYQEKESKRKAGLLPVNKVQTEGSDEEDEDEEE
GDDDEEMNDAPASSSSSDPSEPQGPIVDEDGFELVQKKGNRRR"
gene complement(<331283..>333655)
/locus_tag="AWJ20_3809"
/db_xref="GeneID:30035871"
mRNA complement(<331283..>333655)
/locus_tag="AWJ20_3809"
/product="hypothetical protein"
/transcript_id="XM_018880840.1"
/db_xref="GeneID:30035871"
CDS complement(331283..333655)
/locus_tag="AWJ20_3809"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733491.1"
/db_xref="GeneID:30035871"
/translation="MGNTDSKLRFRTHISRLFELDNISADDRYYWVKYWTEPNSVFDV
FTLLSSADIRKTRDGNLANLETLIKVVYFRLKELRNSRSFPHNDIAPAKELLNCVRIL
TKILPFVYEKDTDEIREWEEKVFWQTTSDKTSDTSHLQGIAPDLINITLDLLFIEGFT
IPIGQANSSIWEPGVGSNTVQQPTHEMLSNRVEILRFLLTLVSDCLYQNQVDVAVKGS
KYLTYVVTNPDKRVVMTVLCSLLNTSLRYSPAGKVPYDHMLISDRNRQLVIYSVQLLV
VLLVYPIPEVPDFSIGSSSRLIPKSGSGRKQDINMYRYFLSKIRHPEDFQFIIDNLLK
LISQPILLTSSLLPGSQTEVQWVTELLALFWELTQCNRKFRDYLYSGSATDFMIFLLA
YVQEKYQDASQREVVRLSAYILLYLSSDSAFVKLLRYKVEVSSGSDDINGNLRRKYIS
SKSKMSIFSGTYGDYLINQLLHPLLSSGSNNDPMFDFLAPTFMEIIYNISPFTINISY
STASNLVQLFSNLISSNYIITASIKEFLLLNILKVFSNLIHFNFRSNKNLLFVILKNE
SKFRFLHNSTKDGDSDGPFVIEDEDGGIDGPSEASSSKSKGKQPVKAASSTPMVSAKD
SSSRVSDLWTLVEPIIMIVEDLRRQIPYTEYISTTNTQETKVQPHIIIDSIGNIPSVR
GVDLDQHEPADFEPIRFNWNSISLGWYESIIWGDIFRNESQLILSTINEVATSLVSPR
IGSVGVWNGTNIKLFRLQEAARQGPSLLRPKGAVDAVAESVLQQFGKLTH"
gene complement(<334250..>334331)
/locus_tag="AWJ20_3810"
/db_xref="GeneID:30035873"
tRNA complement(<334250..>334331)
/locus_tag="AWJ20_3810"
/product="tRNA-Ser"
/db_xref="GeneID:30035873"
gene <334574..>335491
/gene="CDA1"
/locus_tag="AWJ20_3811"
/db_xref="GeneID:30035874"
mRNA <334574..>335491
/gene="CDA1"
/locus_tag="AWJ20_3811"
/product="chitin deacetylase CDA1"
/transcript_id="XM_018880842.1"
/db_xref="GeneID:30035874"
CDS 334574..335491
/gene="CDA1"
/locus_tag="AWJ20_3811"
/inference="similar to AA sequence:KEGG_Orthology:K01452"
/note="Chitin deacetylase; together with Cda2p involved in
the biosynthesis ascospore wall component, chitosan;
required for proper rigidity of the ascospore wall;
GO_component: GO:0005631 - chitosan layer of spore wall
[Evidence ISS] [PMID 8940152]; GO_component: GO:0005628 -
prospore membrane [Evidence IDA] [PMID 24390141];
GO_function: GO:0003824 - catalytic activity [Evidence
IEA]; GO_function: GO:0004099 - chitin deacetylase
activity [Evidence IEA]; GO_function: GO:0004099 - chitin
deacetylase activity [Evidence IDA,ISS] [PMID 8940152];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016810 - hydrolase activity, acting
on carbon-nitrogen (but not peptide) bonds [Evidence IEA];
GO_process: GO:0030476 - ascospore wall assembly [Evidence
IDA,IMP] [PMID 8940152]; GO_process: GO:0005975 -
carbohydrate metabolic process [Evidence IEA,IEA];
GO_process: GO:0006032 - chitin catabolic process
[Evidence IEA]; GO_process: GO:0000272 - polysaccharide
catabolic process [Evidence IEA]; GO_process: GO:0030435 -
sporulation resulting in formation of a cellular spore
[Evidence IEA]"
/codon_start=1
/product="chitin deacetylase CDA1"
/protein_id="XP_018733492.1"
/db_xref="GeneID:30035874"
/translation="MTRLAFFVLIAITASAADITTARAAAAVVAVNSPLQAPLAPSPS
DPFPLWLQELTGQTRWPGETPPYIPMPNIDLSQVPNEIPRRDLGVCQGIERSHCSFDC
YRCVAVDDISTCPTLSQTFDDGPSMHTPKLLMELEYKTTFFTQGINVVRYPDIFRQQH
MAGHLLATHTWSHAHLPGLSNEDIVAQLQWSIWAMNATAGIVPKYFRPPYGAIDDRVR
SISRQFGLVAVFWDRDSYDWRLNDDSKLVEEVVKDIVSWKQESQGGLILEHDSTVKTV
NAGINVSRMLGPSQLTVAECINSNWYQNQ"
gene <337686..>339938
/gene="TMT1"
/locus_tag="AWJ20_3812"
/db_xref="GeneID:30035875"
mRNA <337686..>339938
/gene="TMT1"
/locus_tag="AWJ20_3812"
/product="Ubiquinone/menaquinone biosynthesis
methyltransferase ubiE"
/transcript_id="XM_018880843.1"
/db_xref="GeneID:30035875"
CDS 337686..339938
/gene="TMT1"
/locus_tag="AWJ20_3812"
/inference="similar to AA sequence:KEGG_Orthology:K13621"
/codon_start=1
/product="Ubiquinone/menaquinone biosynthesis
methyltransferase ubiE"
/protein_id="XP_018733493.1"
/db_xref="GeneID:30035875"
/translation="MSSMLTLILPAAAVLALALIITNPSAKAVAQFAWNCFLKPIGHV
GSDQKSALESFYKGQAKIYDTTRKKLLKGRHTMLSLSVSHLKKSKDIVWIDFGGGTGW
NIEKMNEVVPITSFKEIHLVDLSPSLCEVAQNRFAARGWSNVHVHCIDATTFQLPNKE
TRADLITLSYSLSMIPSYYSMIDRICQLVHSDSIFAVVDFYVQSNATLNGKATSVGGV
LLRHVNWLSRTFWRLWFEFDRVYLDSSRRDYLEYRFGTIKSLNMRNKSLGGIPYYIWI
GCDKDRSQDFMYRLNALATESPYLAPSNASSDALANQEQIQIRSKGYEATVINMEHNL
PYPSFYYQSEVWRIYYDAENPHYHQFNNQYIYAFTWEDPREDINLLNLGPSDVVLAIT
SAGDNILTYAAMENPPKRIHGVDLNPHQNHLMELKLASLRCLPFEDVWKIFGDGKHED
FMKILVTKLSPHLSSHALQFWIKNGKKYLDPKTKGLYDSGSTRWAIRIARWLFFALGL
ESDVKRLLKTQTIKEQLKVWNESIRPALFNPIFCKLILGNPVFLWKALGVPVNQANMI
EGGILQYIVDTIDPLISRSLISDDNYFYHLCLTGSYSPKNCPDYITKKGYFNLAKRKG
ALDTIRLHTDTINDVSDRLAPGTLTVAIIMDHMDWFEPTGTLAVEEIQALNKSLAKGG
RVMLRSSSKTPWYIKTFESQGFSCKSAATRFSGTSIDRVNMYASTWICTKLYNTAEKE
RSGSIKPLEL"
gene <341366..>342292
/locus_tag="AWJ20_3813"
/db_xref="GeneID:30035876"
mRNA <341366..>342292
/locus_tag="AWJ20_3813"
/product="hypothetical protein"
/transcript_id="XM_018880844.1"
/db_xref="GeneID:30035876"
CDS 341366..342292
/locus_tag="AWJ20_3813"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733494.1"
/db_xref="GeneID:30035876"
/translation="MDSRITEVQVSLHDDTVSVVSPKEITSRDIAKVLDKEGFDVIVT
DVSEDPEESHHGSSSIWLPSRIRYWQKSRAHLEKCDQCRISKASSESSRSSLKKALHY
FEKEKDITDGSSVIHEEDEDMVVVMDASSDATQFRAIYSIGDMTCAACTTAISTTVKE
QMPELLDIGVDLINKSAVALVPNKQVALKIQEIINDIGYTCELVELTPVESNKSWRLT
ASISGMSCMSCVNAIKDQVGPLPYLQEADISLLTNSGTFTINDLKMADELKEKIQDMG
YDYDLIEVNEVDYAKNKTSRTVNLSIKGMFCE"
gene <342657..>344816
/gene="CCC2"
/locus_tag="AWJ20_3814"
/db_xref="GeneID:30035877"
mRNA <342657..>344816
/gene="CCC2"
/locus_tag="AWJ20_3814"
/product="Cu(2+)-transporting P-type ATPase CCC2"
/transcript_id="XM_018880845.1"
/db_xref="GeneID:30035877"
CDS 342657..344816
/gene="CCC2"
/locus_tag="AWJ20_3814"
/note="Cu(+2)-transporting P-type ATPase; required for
export of copper from the cytosol into an extracytosolic
compartment; has similarity to human proteins involved in
Menkes and Wilsons diseases; protein abundance increases
in response to DNA replication stress; affects TBSV model
(+)RNA virus replication by regulating copper metabolism;
GO_component: GO:0005794 - Golgi apparatus [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0012510 - trans-Golgi network
transport vesicle membrane [Evidence IDA] [PMID 9325307];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0019829 - cation-transporting ATPase
activity [Evidence IEA]; GO_function: GO:0019829 -
cation-transporting ATPase activity [Evidence IDA] [PMID
15078884]; GO_function: GO:0019829 - cation-transporting
ATPase activity [Evidence IMP] [PMID 7708696];
GO_function: GO:0005507 - copper ion binding [Evidence
IEA]; GO_function: GO:0005507 - copper ion binding
[Evidence IDA] [PMID 17961510]; GO_function: GO:0043682 -
copper-transporting ATPase activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA,IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA,IEA]; GO_process: GO:0006812 -
cation transport [Evidence IEA]; GO_process: GO:0006879 -
cellular iron ion homeostasis [Evidence IMP] [PMID
7708696]; GO_process: GO:0060003 - copper ion export
[Evidence IMP] [PMID 7708696]; GO_process: GO:0006825 -
copper ion transport [Evidence IEA,IEA]; GO_process:
GO:0006811 - ion transport [Evidence IEA]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0030001 - metal ion transport [Evidence IEA];
GO_process: GO:0055085 - transmembrane transport [Evidence
IMP] [PMID 7708696]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Cu(2+)-transporting P-type ATPase CCC2"
/protein_id="XP_018733495.1"
/db_xref="GeneID:30035877"
/translation="MDLVPKGSKLRTYFEQPIWAGGVSRSTWALLFLSTPVYFFAADL
FHTKALKEIWSLWRGGASWKRRLFRFGSMNLLMSLGITIAYFASIALLILSATSQPKE
VMGYTSTYFDSVVFLAFFLLMGRCIDAYSKAQTASAVSMLGNLRPDTVNVIEPEQGQT
VLSDYLHNESKAVSIDMVEVGDYVKVLPGERCSVDGIIVDGQSTFDESTLTGESRPVK
HGVGEEIFAGTINTGTGAVVARIKAIEEGSLLNSIVSIVREGQLHRAPIERVAEKITG
IFVPVVTLIAVLTWVIWLALGLSGSLPQRYLDIDVGGWTVWSLQFAISVFVVACPCGI
GLAAPTALFVGSGLAAKYGILARGGGEAFQEGSNIDIICFDKTGTLTEGGEPKITDHH
FTGTISLELLQIANTMEGASTHPLGIAVRNFVQITLEEAKNDTVALYSPAVKKIEEIP
GMGLEAEIDGPEIAKVLLGNERLLEKYNSKVDDETNDLMNKWKAQGKSVILVATSKDR
NNFEIRLVMGAADAVRPESKEVIEALTSRGIECWMISGDNEITAKAVAAQVSIRPENV
IAGVLPAEKSDKVTWLQKSGNSKRTPGGARAIVAMVGDGVNDAPSLSIADVGIAIGSG
SDLALTSAKFVLLKSDLYSLLNLLDISRVVFRRVKFNFAWALIYNLIGIPVAAGVIYP
YHNSRLSPAWASLAMALSSVSVVTSSLLLKLYRPKGQ"
gene complement(<345007..>345756)
/gene="GPM1"
/locus_tag="AWJ20_3815"
/db_xref="GeneID:30035878"
mRNA complement(<345007..>345756)
/gene="GPM1"
/locus_tag="AWJ20_3815"
/product="phosphoglycerate mutase GPM1"
/transcript_id="XM_018880846.1"
/db_xref="GeneID:30035878"
CDS complement(345007..345756)
/gene="GPM1"
/locus_tag="AWJ20_3815"
/inference="similar to AA sequence:KEGG_Orthology:K01834"
/note="Tetrameric phosphoglycerate mutase; mediates the
conversion of 3-phosphoglycerate to 2-phosphoglycerate
during glycolysis and the reverse reaction during
gluconeogenesis; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 3332961]; GO_component: GO:0005758 -
mitochondrial intermembrane space [Evidence IEA];
GO_component: GO:0005758 - mitochondrial intermembrane
space [Evidence IDA] [PMID 22984289]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16962558]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016868 -
intramolecular transferase activity, phosphotransferases
[Evidence IEA]; GO_function: GO:0016853 - isomerase
activity [Evidence IEA]; GO_function: GO:0004619 -
phosphoglycerate mutase activity [Evidence IEA];
GO_function: GO:0004619 - phosphoglycerate mutase activity
[Evidence IDA] [PMID 1386023]; GO_function: GO:0004619 -
phosphoglycerate mutase activity [Evidence IMP] [PMID
3033435]; GO_process: GO:0006094 - gluconeogenesis
[Evidence IMP] [PMID 3033435]; GO_process: GO:0006096 -
glycolytic process [Evidence IEA,IEA,IEA]; GO_process:
GO:0006096 - glycolytic process [Evidence IMP] [PMID
11015729]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]"
/codon_start=1
/product="phosphoglycerate mutase GPM1"
/protein_id="XP_018733496.1"
/db_xref="GeneID:30035878"
/translation="MSTHKLILVRHGQSDWNEKNLFTGWVDVKLSEVGIKEATRAGEL
LKESGLKPDILYTSKLSRAIQTANIALDNADRLFIDVKRNWRLNERHYGALQGKDKAQ
TLAQYGKEQFMTWRRSFDVPPPPIADDSEFSQAHDERYKDVPKEDLPKTESLELVIKR
LLPYWQSDISKDLLAGKTVIVVAHGNSLRALVKHLDNISDEDIAGLNIPTGIPLVYEL
DDNLKPVKKAEYLDPEAAEAGAAAVAAQGSK"
gene <349711..>350178
/gene="YPT1"
/locus_tag="AWJ20_3816"
/db_xref="GeneID:30035879"
mRNA <349711..>350178
/gene="YPT1"
/locus_tag="AWJ20_3816"
/product="Rab family GTPase YPT1"
/transcript_id="XM_018880847.1"
/db_xref="GeneID:30035879"
CDS 349711..350178
/gene="YPT1"
/locus_tag="AWJ20_3816"
/note="Rab family GTPase; involved in the ER-to-Golgi step
of the secretory pathway; complex formation with the Rab
escort protein Mrs6p is required for prenylation of Ypt1p
by protein geranylgeranyltransferase type II
(Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates
unfolded protein response (UPR) by promoting the decay of
HAC1 RNA; GO_component: GO:0005794 - Golgi apparatus
[Evidence IEA]; GO_component: GO:0000139 - Golgi membrane
[Evidence IEA]; GO_component: GO:0000139 - Golgi membrane
[Evidence IDA] [PMID 10747087]; GO_component: GO:0005795 -
Golgi stack [Evidence IDA] [PMID 12802060]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0031410 - cytoplasmic vesicle [Evidence IDA] [PMID
23129774]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IDA] [PMID 10747087]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0000407 -
pre-autophagosomal structure [Evidence IDA] [PMID
20375281]; GO_component: GO:0005802 - trans-Golgi network
[Evidence IDA] [PMID 20059749]; GO_function: GO:0005525 -
GTP binding [Evidence IEA,IEA]; GO_function: GO:0003924 -
GTPase activity [Evidence IDA] [PMID 3311726];
GO_function: GO:0003924 - GTPase activity [Evidence IDA]
[PMID 9447979]; GO_function: GO:0000149 - SNARE binding
[Evidence IDA,IPI] [PMID 18388317]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0090114 - COPII-coated vesicle budding
[Evidence IMP] [PMID 15689495]; GO_process: GO:0032258 -
CVT pathway [Evidence IMP] [PMID 20375281]; GO_process:
GO:0006888 - ER to Golgi vesicle-mediated transport
[Evidence IMP] [PMID 15689495]; GO_process: GO:0006888 -
ER to Golgi vesicle-mediated transport [Evidence IMP]
[PMID 2504726]; GO_process: GO:0006888 - ER to Golgi
vesicle-mediated transport [Evidence IMP] [PMID 7593181];
GO_process: GO:0048194 - Golgi vesicle budding [Evidence
IGI] [PMID 18388317]; GO_process: GO:0048211 - Golgi
vesicle docking [Evidence IMP] [PMID 9545229]; GO_process:
GO:0035494 - SNARE complex disassembly [Evidence IMP]
[PMID 9157884]; GO_process: GO:0034498 - early endosome to
Golgi transport [Evidence IMP] [PMID 20059749];
GO_process: GO:0032456 - endocytic recycling [Evidence
IMP] [PMID 20059749]; GO_process: GO:0016236 -
macroautophagy [Evidence IMP] [PMID 20375281]; GO_process:
GO:1990261 - pre-mRNA catabolic process [Evidence IMP]
[PMID 22844259]; GO_process: GO:0006461 - protein complex
assembly [Evidence IDA] [PMID 8603910]; GO_process:
GO:0015031 - protein transport [Evidence IEA,IEA];
GO_process: GO:1900101 - regulation of endoplasmic
reticulum unfolded protein response [Evidence IMP] [PMID
22844259]; GO_process: GO:0006890 - retrograde
vesicle-mediated transport, Golgi to ER [Evidence IGI,IMP]
[PMID 18388317]; GO_process: GO:0007264 - small GTPase
mediated signal transduction [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]; GO_process:
GO:0016192 - vesicle-mediated transport [Evidence IEA]"
/codon_start=1
/product="Rab family GTPase YPT1"
/protein_id="XP_018733497.1"
/db_xref="GeneID:30035879"
/translation="MKLQIWDTAGQEHFRAVTRSYFRNAAGCILVYDVTRRQTFDQVS
MWLNDIREQAHEEINICLVGNKSDLEDQRQVPYEEAKQWADSNGISHFLETSAKTGDN
VLEAYTSVAQSIHSKIVGGRYNLQDKGHGVKANNSGLINLNVDASTKRKGGCC"
gene <350571..>352538
/gene="PUF6"
/locus_tag="AWJ20_3817"
/db_xref="GeneID:30035880"
mRNA <350571..>352538
/gene="PUF6"
/locus_tag="AWJ20_3817"
/product="Puf6p"
/transcript_id="XM_018880848.1"
/db_xref="GeneID:30035880"
CDS 350571..352538
/gene="PUF6"
/locus_tag="AWJ20_3817"
/inference="similar to AA sequence:KEGG_Orthology:K14844"
/note="Pumilio-homology domain protein; binds the 3' UTR
of ASH1 mRNA and represses its translation, resulting in
proper asymmetric localization of ASH1 mRNA; required at
post-transcriptional step for efficient
retrotransposition; absence results in decreased Ty1
Gag:GFP protein levels; co-sediments with the 60S
ribosomal subunit and is required for its biogenesis;
GO_component: GO:0005934 - cellular bud tip [Evidence
IEA]; GO_component: GO:0015934 - large ribosomal subunit
[Evidence IDA] [PMID 19806183]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003723 - RNA binding [Evidence IEA,IEA];
GO_function: GO:0003730 - mRNA 3'-UTR binding [Evidence
IDA] [PMID 15198983]; GO_function: GO:0003729 - mRNA
binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0000900 - translation repressor activity, nucleic acid
binding [Evidence IDA] [PMID 15198983]; GO_process:
GO:0017148 - negative regulation of translation [Evidence
IDA] [PMID 15198983]; GO_process: GO:0006417 - regulation
of translation [Evidence IEA]; GO_process: GO:0042273 -
ribosomal large subunit biogenesis [Evidence IMP] [PMID
19806183]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Puf6p"
/protein_id="XP_018733498.1"
/db_xref="GeneID:30035880"
/translation="MGKTAVKLSTKRKAVAVESRKPVTNGKLKKAKKEEEVVEESEEN
EDDFASSDDESINLSSDDSDEDELDKDDDEEDELDQDEEVDEEGNGSEDGEEDEEGLA
DDGSKTARSKEQRAEQKKLQQERKLQRSGGVEIQQIKNIWERLRVKTGVPTEVRKKLV
DQAWELCHDKVKELVFKHDASRVVQTIFKYADKEKRLAITKALKGSYVELAKSSYGKY
LLVKLLHYGSSDVRDGVLSEMHGSFRKLIKHKEGAYVIEDAYRDYSTAAHKSQIISEF
YGSEFAIFKDIAKDKSLEDIIKENPDKRPYLMKNLNEVITSAVNKGSIGFTIIHAAML
EYVKNIDATGSEREVFIDLITEQFAEIVHTNEGSQVACRVLSIATAKERKGLVRSLKP
FASKLASDEYGYLVLIALFRSVDDTVLVSKSFTADLKENINSLIVSKTGRRAFLYLLL
GLSPRYFTPTIIKKFNQLDELKAATSKKDDEVRRTELLKAFSPLFFEAISANTRTILK
ESLGAQFVAEALLYGIGEDDRQKALESVASAFGDSSPDADNGTHLIHEPFSTRLLRTL
VQDGHWDAKEKRANVVSSPTNFKALLLTKVLEFPAEWATGDGSFVVVSLVENLESADK
ATLVKVLKKHKKAITAASADNKGAKLLVEHL"
gene complement(<352859..>353602)
/gene="HAP3"
/locus_tag="AWJ20_3818"
/db_xref="GeneID:30035881"
mRNA complement(<352859..>353602)
/gene="HAP3"
/locus_tag="AWJ20_3818"
/product="Hap3p"
/transcript_id="XM_018880849.1"
/db_xref="GeneID:30035881"
CDS complement(352859..353602)
/gene="HAP3"
/locus_tag="AWJ20_3818"
/note="Subunit of the Hap2p/3p/4p/5p CCAAT-binding
complex; complex is heme-activated and glucose-repressed;
complex is a transcriptional activator and global
regulator of respiratory gene expression; contains
sequences contributing to both complex assembly and DNA
binding; GO_component: GO:0016602 - CCAAT-binding factor
complex [Evidence IDA,IPI] [PMID 2832951]; GO_component:
GO:0005622 - intracellular [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence IEA,IEA]; GO_function:
GO:0000978 - RNA polymerase II core promoter proximal
region sequence-specific DNA binding [Evidence IDA] [PMID
7828851]; GO_function: GO:0001077 - RNA polymerase II core
promoter proximal region sequence-specific DNA binding
transcription factor activity involved in positive
regulation of transcription [Evidence IDA] [PMID 2832951];
GO_function: GO:0046982 - protein heterodimerization
activity [Evidence IEA]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IEA]; GO_process:
GO:0000436 - carbon catabolite activation of transcription
from RNA polymerase II promoter [Evidence IDA] [PMID
2832951]; GO_process: GO:0043457 - regulation of cellular
respiration [Evidence IMP] [PMID 2182199]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA,IEA]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Hap3p"
/protein_id="XP_018733499.1"
/db_xref="GeneID:30035881"
/translation="MDKSPPDSSKNFELREQDKWLPIANVSRIMKNALPESAKVSKEA
KECVQECVSEFVSFITSEASEKCSAEKRKTVNGEDILFAMTSLGFENYAEVLKIYLAK
YREFQVLKQEREGDRKPSGGSGAGLGRLGNGGSSVSVGLDEDEDDDMDSGSGGVVMDN
ASESLAADGAVAEMLNTDGEHDQEHAEIKVEGQSGDGSEGAVATTADTYTNYDDSTYT
TNDHAPAATDDPSIYEDYGQYTNVDMNQF"
gene complement(<354966..>356045)
/locus_tag="AWJ20_3819"
/db_xref="GeneID:30035882"
mRNA complement(<354966..>356045)
/locus_tag="AWJ20_3819"
/product="hypothetical protein"
/transcript_id="XM_018880850.1"
/db_xref="GeneID:30035882"
CDS complement(354966..356045)
/locus_tag="AWJ20_3819"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733500.1"
/db_xref="GeneID:30035882"
/translation="MVGEAPKLELQSLEFEKRMVLLTYSLALMMLAETAVTSRYGDGN
QDNDRKWKLATSHLIKSQSIVWYLVANSNSYKGQPIPPQLQNQTGFITGLQTLIGGSL
HLLIIYKDLERELQESSDGYNSSSTTTTSRNLLSRVAIYAADKFGAASQLISSSSSSF
SSSGGTGSDLSSSGSTEPTKSKMRSAMSRIAPSSHNGSSSSSSSSHNSSFSLGNSSDT
LSGWLSEARKFCIADTYKLSAIDQAQKGQTGQAIGFLTAALELLGANSKLKKVVSSTA
KPGTIQYQQDQLKQTILPILHSYTTENNNYTFQTIPKASELESNRPSGRQVVAAEQPW
VPPSSLISDSVSETLTPSSSSRTYY"
gene <357300..>360764
/gene="VPS3"
/locus_tag="AWJ20_3820"
/db_xref="GeneID:30035884"
mRNA <357300..>360764
/gene="VPS3"
/locus_tag="AWJ20_3820"
/product="CORVET complex subunit VPS3"
/transcript_id="XM_018880852.1"
/db_xref="GeneID:30035884"
CDS 357300..360764
/gene="VPS3"
/locus_tag="AWJ20_3820"
/codon_start=1
/product="CORVET complex subunit VPS3"
/protein_id="XP_018733501.1"
/db_xref="GeneID:30035884"
/translation="MESDPYIIASKQRTHSKKRRPVSQIVILPTISRALVLSQSTTSV
FTLPEFAPCSNVGSMRDVNALSRDIDDFTVLNERGDSEFAEVTVFSKSAIRLVKVFGE
SLKLAGNIEYPGVLNGVRRSSYALVVNNESYDLLDLKHVRKVPLFPLRAAGEDEDQDS
ETREEDTYAQKQPGTTTETAGTEGETNASPTVEQSAETEPVGTTLTNKQVPEIAKEEG
DDSKTGTEESKTITETSAQSTTEEVDKAKDELTEPGSEADKKSNVETDPTNTIKVEED
AGMEVSVDSTTEVTTEPSTQPAPEVKVGETATSEGSTTEPSTQVATELKIGGTATTEG
SHIQRPRAGKGHAPAVSIHSIQSTGSVESAESKPSSSPVSTTSATFPSMEGVPTPTTE
VEGSKSDHSATGKSKSKKHADPIKPIIVPVASDEFLVTSGTSAVDRAMGLVVNVDGEI
SRGTIAWSKYPSSVAVDYPYVAAVIDNQVQLHSLHDQKLVQTVEFPSKPLVTTVLAPY
KTPYTVLVNKIVLAPLAVEDIKSPDVVSRMSSERDTACKISICSSTLFAYTPEAGVEC
LIPTPRFLSLEKLVLVNGQVDDVYDEIERMDFSTELAVTQLQYLNLLIGLYYLREANH
TPSHFSDVTKTWLVASLDPRIMIFIFDKKNVKGEVWVFNGVISFIETLQKTYSSSMSK
LSENVVSFLKTFYKDWFKKREMASLGLADKENVFKSLELSYLAFLLKCEEKSKASQHD
LIHAIDNEIVLATDESIELLRQQKKFFYLSHMYNKLKMPSELLSCWKEMLLETNPAFA
NGETTMAEYLINECEDEGLVWDYGAWLVQTYPKTGIKVFTSPDLRVNINENQVVAVLK
SMNNDEAWRQYLKFLVYEKNQTLFASDLATVMLLDIVDKISKNPDVRKSLMDNYDIYK
ALPVPKPAYIPFLRNKLQNSQPVVPTKSKGLLLKSTIAAPAASLSPFEEVMKMRLELM
ELVDSTSDFDYTSLMHLSQDEPDTLLIEQCVVFGHLGIHEQCLDILCNRLTDYKTAID
YCIYGRIPISMTILEPLDSQIQKDLFRQLFLRYLKVPDPGTRIACTRILLEQWGNRLD
VSLVLSNLPDNWPVEVVSGYLSQFFKRTMADQNQASVDRSLARSENLFISQQLLELSK
IAYTPSDI"
gene complement(<360932..>361576)
/locus_tag="AWJ20_3821"
/db_xref="GeneID:30035885"
mRNA complement(<360932..>361576)
/locus_tag="AWJ20_3821"
/product="hypothetical protein"
/transcript_id="XM_018880853.1"
/db_xref="GeneID:30035885"
CDS complement(360932..361576)
/locus_tag="AWJ20_3821"
/inference="similar to AA sequence:KEGG_Orthology:K03247"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733502.1"
/db_xref="GeneID:30035885"
/translation="MFAYQAENADSVVLVYDTAKSNQGGLALRAYRLSNSYLNAKKDG
KFTTENLVKNELSYSNIFQELPIDVKNSHLVTLYLHSAKEATENVKDYDDLAISVDQF
LEQNIEGIFDSVDEFHYDQGNYNYYQRQLTREKLKIQQWQQKRKAENTALEQQGKPLL
STDDYKTLFRLPEEPSRLDNLLISAQINQYCTQIEEFGATVVPKLFATQKVLEG"
gene <362367..>363341
/gene="PRP18"
/locus_tag="AWJ20_3822"
/db_xref="GeneID:30035886"
mRNA <362367..>363341
/gene="PRP18"
/locus_tag="AWJ20_3822"
/product="Prp18p"
/transcript_id="XM_018880854.1"
/db_xref="GeneID:30035886"
CDS 362367..363341
/gene="PRP18"
/locus_tag="AWJ20_3822"
/inference="similar to AA sequence:KEGG_Orthology:K12817"
/note="Splicing factor and component of snRNP U5; factor
involved in the positioning of the 3' splice site during
the second catalytic step of splicing; interacts with
Slu7p; GO_component: GO:0071021 - U2-type
post-spliceosomal complex [Evidence IDA] [PMID 12212850];
GO_component: GO:0046540 - U4/U6 x U5 tri-snRNP complex
[Evidence IDA] [PMID 8474454]; GO_component: GO:0005682 -
U5 snRNP [Evidence IDA] [PMID 8474454]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005681 - spliceosomal complex [Evidence IEA,IEA];
GO_function: GO:0000386 - second spliceosomal
transesterification activity [Evidence IDA] [PMID
12212850]; GO_function: GO:0000386 - second spliceosomal
transesterification activity [Evidence IGI] [PMID
17626844]; GO_process: GO:0008380 - RNA splicing [Evidence
IEA,IEA]; GO_process: GO:0000350 - generation of catalytic
spliceosome for second transesterification step [Evidence
IDA] [PMID 12212850]; GO_process: GO:0006397 - mRNA
processing [Evidence IEA]; GO_process: GO:0000398 - mRNA
splicing, via spliceosome [Evidence IMP] [PMID 12403466];
GO_process: GO:0071048 - nuclear retention of unspliced
pre-mRNA at the site of transcription [Evidence IGI,IMP]
[PMID 14718167]"
/codon_start=1
/product="Prp18p"
/protein_id="XP_018733503.1"
/db_xref="GeneID:30035886"
/translation="MDFSALLAKEIAKKKAQAAAVTNLKTESADGPGQKPKSYVRKAE
LEELREKEYRKEQEAKQAAARKRQLEQELEHEREKENEAKRLKRRGNDGSNSNKSETE
RSDSEIIARLEELNEPTSVPGESEKGRKNRLARIEKKLELQERLRKEEEEDARTDPTI
VPQDIKDNEPKVYIQMRVYIKQLLRKWELYLEQNQEETKSIYKEASKDIEILLGHLRN
KSLSKQIFPSLATLLYYLQTKKFRDANDTYIQLSIGNVAWPIGVIGVAIHERSAQSKI
TGIHNTSSEGSVQIAHIMSDDETRRWLTAVKRLITFCEKTSKTSSVTD"
gene complement(<363718..>364554)
/gene="SGT1"
/locus_tag="AWJ20_3823"
/db_xref="GeneID:30035887"
mRNA complement(<363718..>364554)
/gene="SGT1"
/locus_tag="AWJ20_3823"
/product="Sgt1p"
/transcript_id="XM_018880855.1"
/db_xref="GeneID:30035887"
CDS complement(363718..364554)
/gene="SGT1"
/locus_tag="AWJ20_3823"
/inference="similar to AA sequence:KEGG_Orthology:K12795"
/note="Cochaperone protein; regulates activity of adenylyl
cyclase Cyr1p; involved in kinetochore complex assembly;
associates with the SCF (Skp1p/Cdc53p/F box protein)
ubiquitin ligase complex; acts as a linker between Skp1p
and HSP90 complexes; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0000151 - ubiquitin ligase complex [Evidence IDA] [PMID
10445024]; GO_function: GO:0051087 - chaperone binding
[Evidence ISS] [PMID 12456005]; GO_function: GO:0051087 -
chaperone binding [Evidence IDA] [PMID 16945921];
GO_function: GO:0030674 - protein binding, bridging
[Evidence IDA] [PMID 16945921]; GO_process: GO:0019933 -
cAMP-mediated signaling [Evidence IGI,IPI] [PMID
12456005]; GO_process: GO:0007049 - cell cycle [Evidence
IEA]; GO_process: GO:0051382 - kinetochore assembly
[Evidence IDA] [PMID 10445024]; GO_process: GO:0006461 -
protein complex assembly [Evidence IGI,ISS] [PMID
12456005]; GO_process: GO:0006461 - protein complex
assembly [Evidence IDA] [PMID 15090617]; GO_process:
GO:0016567 - protein ubiquitination [Evidence TAS] [PMID
12456005]; GO_process: GO:0051726 - regulation of cell
cycle [Evidence TAS] [PMID 11847307]"
/codon_start=1
/product="Sgt1p"
/protein_id="XP_018733504.1"
/db_xref="GeneID:30035887"
/translation="MKIQSHLKSVEDEDDLIVSIQDIPKIQDLSDDESGRKEVEKALD
VQPEKQSGISVSTPEYPAPDTVRHDWFQMGNKCDVSIFIKNSPKEDVTVEFTESSATI
KFPLASGQLYEYNIGPFSHEIEPSKSTCKVFSTKIELGIVKKVNTSWATLLSPEGERP
LNNKVPLSLDEMRTALEKTAKDSSNSSSEASTTNSTRPLVPSFEKNWEKMAAEALGDE
EQQPDDFFKQLYGNADDDTRKAMLKSYTESGGTTLSTNWEDVKKAPVPVSPPSGMEAK
KF"
gene <365586..>367052
/gene="NOB1"
/locus_tag="AWJ20_3824"
/db_xref="GeneID:30035888"
mRNA <365586..>367052
/gene="NOB1"
/locus_tag="AWJ20_3824"
/product="Nob1p"
/transcript_id="XM_018880856.1"
/db_xref="GeneID:30035888"
CDS 365586..367052
/gene="NOB1"
/locus_tag="AWJ20_3824"
/inference="similar to AA sequence:KEGG_Orthology:K11883"
/note="Protein involved in proteasomal and 40S ribosomal
subunit biogenesis; required for cleavage of the 20S
pre-rRNA to generate the mature 18S rRNA; cleavage is
activated by Fun12p, a GTPase and translation initiation
factor; relocalizes from nucleus to nucleolus upon DNA
replication stress; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 12588997]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 22842922]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IEA,IEA];
GO_component: GO:0005730 - nucleolus [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
12502737]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 12588997]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22842922]; GO_component: GO:0030688 -
preribosome, small subunit precursor [Evidence IDA] [PMID
12588997]; GO_function: GO:0004521 - endoribonuclease
activity [Evidence IDA] [PMID 19801658]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0004518 - nuclease activity
[Evidence IEA]; GO_function: GO:0070181 - small ribosomal
subunit rRNA binding [Evidence IDA] [PMID 19706509];
GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
IDA] [PMID 19801658]; GO_process: GO:0000462 - maturation
of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IEP,IMP] [PMID 12588997];
GO_process: GO:0000462 - maturation of SSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 15388878]; GO_process:
GO:0090305 - nucleic acid phosphodiester bond hydrolysis
[Evidence IEA]; GO_process: GO:0043248 - proteasome
assembly [Evidence IMP,IPI] [PMID 12502737]; GO_process:
GO:0042274 - ribosomal small subunit biogenesis [Evidence
IDA] [PMID 12588997]; GO_process: GO:0042274 - ribosomal
small subunit biogenesis [Evidence IPI] [PMID 12628929];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA]"
/codon_start=1
/product="Nob1p"
/protein_id="XP_018733505.1"
/db_xref="GeneID:30035888"
/translation="MASINSLILDAGPLLTQTFSDLHSRAEKFFTTPSVYNEIKDERA
RQNLLLWGEELVVRQPKPSSIAAVSEFSKKTGDYAVLSSTDMHILALCYELEVELNGG
DWRLRKVPGQKNINGAKEVKHSETQKSESTDSSVSKVKESNEQTAEPVSDLTKTESKK
ESSETTATEPEANPEETEDDGWSVVAAKPRKQKKGKKRNDLWSKPAQVPVIPSEPTTT
DSAQPESEVDNQQITEATATEESQPPAEENVETQDSFVEEIIEEDYDDEDADDWITTE
NLQETLEKAGGDKLEKVETKTMKAAMSTGDFAMQNVALQMGLNLVNPTNGKHIKRVKN
YMLRCHACFKLTAFPKDGRPRQFCPRCGGATLLRCTVQVSDSGNIQVFLKKNMQWSSR
GDKFSLPTPQSRNARKQRHESDPILLREDQKEYQKAVKDSMWKKRQNEKLLDEWIGTG
SADSVGSPFAISGYKRDATKHTGVRVGRGRYVNDSKRK"
gene <371591..>372541
/gene="RSR1"
/locus_tag="AWJ20_3825"
/db_xref="GeneID:30035889"
mRNA <371591..>372541
/gene="RSR1"
/locus_tag="AWJ20_3825"
/product="Ras family GTPase RSR1"
/transcript_id="XM_018880857.1"
/db_xref="GeneID:30035889"
CDS 371591..372541
/gene="RSR1"
/locus_tag="AWJ20_3825"
/inference="similar to AA sequence:KEGG_Orthology:K07836"
/note="GTP-binding protein of the Ras superfamily;
required for bud site selection, morphological changes in
response to mating pheromone, and efficient cell fusion;
localized to the plasma membrane; significantly similar to
mammalian Rap GTPases; GO_component: GO:0005935 - cellular
bud neck [Evidence IDA] [PMID 15282802]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 8723349]; GO_function: GO:0005525 - GTP binding
[Evidence IEA,IEA]; GO_function: GO:0003924 - GTPase
activity [Evidence IDA] [PMID 1910037]; GO_function:
GO:0003924 - GTPase activity [Evidence TAS] [PMID
9759491]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_function: GO:0004871 - signal
transducer activity [Evidence TAS] [PMID 9891811];
GO_process: GO:0006184 - GTP catabolic process [Evidence
IEA]; GO_process: GO:0007120 - axial cellular bud site
selection [Evidence TAS] [PMID 2065354]; GO_process:
GO:0007120 - axial cellular bud site selection [Evidence
TAS] [PMID 2690082]; GO_process: GO:0007121 - bipolar
cellular bud site selection [Evidence TAS] [PMID 2065354];
GO_process: GO:0007121 - bipolar cellular bud site
selection [Evidence TAS] [PMID 2690082]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0051301 - cell division [Evidence IEA]; GO_process:
GO:0000282 - cellular bud site selection [Evidence TAS]
[PMID 9759491]; GO_process: GO:0007165 - signal
transduction [Evidence IEA]; GO_process: GO:0007264 -
small GTPase mediated signal transduction [Evidence IEA];
GO_process: GO:0007264 - small GTPase mediated signal
transduction [Evidence TAS] [PMID 9891811]"
/codon_start=1
/product="Ras family GTPase RSR1"
/protein_id="XP_018733506.1"
/db_xref="GeneID:30035889"
/translation="MRDYKVVVLGAGGVGKSALTVQFVQGVYLESYDPTIEDSYRKQC
LIDNRSCTLEILDSAGVEQFTAMRELYIKNGEGFILVYSVTNESSLQELLELREQVIR
IKDNANVPIVLVGNKCDLMDDRVVPAKVGVQVADSWGKVPFYETSAKYRLNVEEVFVD
LVRQIMRRDSGFGSSLGMRQGSIDESTKKYILGHGHNNSSNSHYYSSHGRQLSHNGSN
NASSVALAAATHTASNSTSSTFSSRSNSHTQTHSAASLEGFDHAFLTTDPAAKSTRNK
LLRHKPSNRKLSGKASTLSLRRNHANPASAGQKDKDCLIM"
gene complement(<373013..>374818)
/locus_tag="AWJ20_3826"
/db_xref="GeneID:30035890"
mRNA complement(<373013..>374818)
/locus_tag="AWJ20_3826"
/product="methylcrotonoyl-CoA carboxylase beta chain,
mitochondrial precursor"
/transcript_id="XM_018880858.1"
/db_xref="GeneID:30035890"
CDS complement(373013..374818)
/locus_tag="AWJ20_3826"
/inference="similar to AA sequence:KEGG_Orthology:K01969"
/codon_start=1
/product="methylcrotonoyl-CoA carboxylase beta chain,
mitochondrial precursor"
/protein_id="XP_018733507.1"
/db_xref="GeneID:30035890"
/translation="MLQWSLRSISSLGHGVRSSSGLHFGLARCTSISGVHGVKIESRL
ASTASLFASKYQTESLQTLPTAISNTSPEFQEKAAAMNELISEIQAIHNHVTDVNEIP
EKIRTKHLARKKLLPRDRIAALIDPGTSFMDLSLLAGHELYEGEDIRGGGIITGIGIV
QGVKCLIIANDSTVKGGTYYPITVRKHLRAQEIAQENHMPCIYLSDSGGANLPHQSDV
FPAHNHFGHIFYQQSRMSSMGIPQLALVLGPCTAGGAYVPAMSDESIIVRGTGHIFLA
GPPLVKAATGEVVSAEDLGGGEMHASVSGVVDYLAETEEEGIALLRQSVGNLNWPKVE
TLASSAQPEKTIDEPLYPPEELGGVVGTNLKVAYNVREVIARIVDGSRFSEFKKKYGT
SLVCGWSEIWGHKVGILANNGPLFSDSSLKGAHFIQLCKQRDIPLVFLQNISGFMVGS
DAERGGIAKNGAKLVNAVVGAGVEKYTVIIGGSYGAGNYGMCGRAYKPRFLWSWPNAR
TAVMGAEQLASVMDSVGSASNKAKSDALRDRIERESLVKFGSARLWDDGVILPQQTRQ
YLGQALEIRAQSRVVQATRFAQKAAAPHDFGIWRM"
gene <375670..>376845
/locus_tag="AWJ20_3827"
/db_xref="GeneID:30035891"
mRNA <375670..>376845
/locus_tag="AWJ20_3827"
/product="isovaleryl-CoA dehydrogenase, mitochondrial
precursor"
/transcript_id="XM_018880859.1"
/db_xref="GeneID:30035891"
CDS 375670..376845
/locus_tag="AWJ20_3827"
/inference="similar to AA sequence:KEGG_Orthology:K00253"
/note="Fungal Genetics Stock Center 12071"
/codon_start=1
/product="isovaleryl-CoA dehydrogenase, mitochondrial
precursor"
/protein_id="XP_018733508.1"
/db_xref="GeneID:30035891"
/translation="MSSRAYSAAAALDVEELREATIAFCKEEITREVAAATDSNNSFP
NELWPKLGEAGLLGITASPEYGGLGQGYLAHSVVIEELSRASGSIALSYAAHSNLCIN
QLTLHTTPEQGAKYLPDLISGKKIGALAMSEAGSGSDVVSMKTTATLSDDGSGYYLNG
TKMWITNGPDADTLVVYAKTDPKAGSKGITTFIVEKGMPGFSCTRKLDKLGMRGSNTG
ELVFDNVFIPKENVLGKVGGGVYVLMSGLDLERLVLAAGPVGLMKAALDETLPYVHTR
LQFGQPIAHNQLIQGKLADMYTKYATARAFLYETARAADAGHKDTKDCAAVILYAAER
ATEVCLDAIQCLGGMGYVNETVPGRLLRDAKLYEIGAGTSEIRRMVIGRAFNKEYRQ"
gene <380125..>383535
/gene="OSH2"
/locus_tag="AWJ20_3828"
/db_xref="GeneID:30035892"
mRNA <380125..>383535
/gene="OSH2"
/locus_tag="AWJ20_3828"
/product="oxysterol-binding protein related protein OSH2"
/transcript_id="XM_018880860.1"
/db_xref="GeneID:30035892"
CDS 380125..383535
/gene="OSH2"
/locus_tag="AWJ20_3828"
/note="Member of an oxysterol-binding protein family with
seven members; in S. cerevisiae, family members have
overlapping, redundant functions in sterol metabolism and
collectively perform a function essential for viability;
OSH2 has a paralog, SWH1, that arose from the whole genome
duplication; GO_component: GO:0005935 - cellular bud neck
[Evidence IDA] [PMID 11408574]; GO_component: GO:0032541 -
cortical endoplasmic reticulum [Evidence IDA] [PMID
20008566]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IDA] [PMID 12727870]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 11408574]; GO_function: GO:0005545 -
1-phosphatidylinositol binding [Evidence ISS] [PMID
11238399]; GO_function: GO:0008289 - lipid binding
[Evidence IEA]; GO_function: GO:0008289 - lipid binding
[Evidence IDA] [PMID 20008566]; GO_function: GO:0008142 -
oxysterol binding [Evidence ISS] [PMID 11238399];
GO_function: GO:0015248 - sterol transporter activity
[Evidence IDA] [PMID 20008566]; GO_process: GO:0035621 -
ER to Golgi ceramide transport [Evidence IMP] [PMID
24213531]; GO_process: GO:0006897 - endocytosis [Evidence
IGI] [PMID 15173322]; GO_process: GO:0006887 - exocytosis
[Evidence IGI] [PMID 17004323]; GO_process: GO:0006869 -
lipid transport [Evidence IEA]; GO_process: GO:0030011 -
maintenance of cell polarity [Evidence IGI] [PMID
17004323]; GO_process: GO:0015918 - sterol transport
[Evidence IGI] [PMID 16585271]; GO_process: GO:0015918 -
sterol transport [Evidence IDA] [PMID 20008566];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="oxysterol-binding protein related protein OSH2"
/protein_id="XP_018733509.1"
/db_xref="GeneID:30035892"
/translation="MYGRQEIALLLLNQPQINDTIVNSKGKQPVELSKTPQIAEAMQI
ARAQYVEKVAFLLKKYLDEENTAALEELLSIPRASALLDINGQDPVTGGTVLHDFVRK
KNKKMVEFILSHGGDPFKRDFKGVLPIEVTKDDTIKKMLKAAKKEQTVIIGNGDKAGG
SGPGGAVAGGPRVSGESPNMKGYLKKWTNFTGGYKLRWFVLENGVLSYYKRQDDIQRA
CRGSVNLKEAKLHLDSSEKLRFEVVIRGSNSKFHLKGNHPVETNRWVWALQNAIQYAK
DQDRLKNGSGTALSASSTGSTDLRRSGTSGSVSSARTNATTSTTATGVTRSGSAGAAA
RGDENSTLTVPNTSSGSSRISSSSRRSPSSSSHSRSHSASEMFASTNSKIISGTMNLH
DDAESRGLGGIGLNSSTKLVDDILPEDEDVVDDDDDDYSEGLTDPESFDEEIGNGSGL
KWGRFEDSITLEANAIRDLLTLIRAKNQKNIDNQPGGLTSKELDDSLSAMSASIETLE
SVAKQHAKQFNAQERYYQQKIDQAETKERLWAKNLRDLELEHQKIQGDLHSALQKRKE
ATKLLREAQSAQARVTSPSDNSGVSTTAAAAGSAMAGVVAGVGAGIAATTASSDEGST
SKTGDSVPPNPTITAAGQASKSDINRPTITSNEESDDSDDDDDEFFDTLPEVTDTLDE
KGASLATIHESTSAETLAVESDLTSVQEKKKQLITGQDSFAGYEDGPRKRLKLDADDR
PKISLWGVLKSLIGKDMTKMTLPVSFNECTNLLQRSAEDMEYTDILDRGAKIVDDPAL
RLAHVAAFAASSYSSTINRIAKPFNPLLGETFEYCRPDCGYRLFSEQVSHHPPIGALY
AEAPKWDFYGASNVKSQFYGRSFDINPLGRWYLTLRPDAGAGVEEELYSFKKVTSSVV
GIITGSPIVDNYGDMDIINHTLGYKCTVNFKPRGWSGKNAYELKGRIVSPSGKEEWIV
GGRWNDKIIARRIVKGQESTGDGVKLPAGDSNAPTSSANINAILLWKVHDRPPAPFNL
TAFAITLNDLPDKLARYIAPTDTRLRPDQRAMEEGRYDAAADEKKRVEEKQRAARRLR
EQSGVEYKPLWFKKDVHPVTGEEYYTPTRDYWQYRQNKSIKTIAPDIF"
gene <387080..>388555
/gene="MEP3"
/locus_tag="AWJ20_3829"
/db_xref="GeneID:30035893"
mRNA <387080..>388555
/gene="MEP3"
/locus_tag="AWJ20_3829"
/product="ammonium permease MEP3"
/transcript_id="XM_018880861.1"
/db_xref="GeneID:30035893"
CDS 387080..388555
/gene="MEP3"
/locus_tag="AWJ20_3829"
/inference="similar to AA sequence:KEGG_Orthology:K03320"
/note="Ammonium permease of high capacity and low
affinity; belongs to a ubiquitous family of cytoplasmic
membrane proteins that transport only ammonium (NH4+);
expression is under the nitrogen catabolite repression
regulation ammonia permease; MEP3 has a paralog, MEP1,
that arose from the whole genome duplication;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence TAS] [PMID 11486013]; GO_component: GO:0005886 -
plasma membrane [Evidence ISS] [PMID 9234685];
GO_function: GO:0008519 - ammonium transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0008519 - ammonium transmembrane transporter activity
[Evidence IDA,IMP,ISS] [PMID 9234685]; GO_process:
GO:0072488 - ammonium transmembrane transport [Evidence
IEA]; GO_process: GO:0015696 - ammonium transport
[Evidence IEA,IEA]; GO_process: GO:0015696 - ammonium
transport [Evidence IMP,ISS] [PMID 9234685]; GO_process:
GO:0019740 - nitrogen utilization [Evidence IMP] [PMID
9234685]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="ammonium permease MEP3"
/protein_id="XP_018733510.1"
/db_xref="GeneID:30035893"
/translation="MHSYKVAEDLNRARLGIVARGDSSTNFNFDTGDVGYLMVSTCMV
FIMIPGLGFLYSGLARRKSALSMIWFTLSAGCVGIFQWYFWGYSLTFSPTATNGYIGN
LYQFGMRHVLGGDATYPELLFAGYQGMFQAVTVAIVMGGAAERGRLFPAMVVTFLWAT
LVYCPLACWAWGPNGWAGAQWGVLDFAGGGPVEIGSGVAGLAYSIALGRRKEQLLLNF
RPHNVSLVTLGTVLLWFGWLGFNGGSALGANLRAVYAVWNSNLCAAFGAMAWCLLDFR
LEKKWSTVAICSGIISGLVAATPAAGNIPMWASVILGATAGIVCNFSTQIKYLIHADD
SMDTFAEHAMAGIIGLLFNGLFAETWIIGLDGVTVHPGGWMSHNWKQLYKQFAYLCAC
MGYCFVVTLLICYAVNYIPGCHLRVTEDGERGGIDEDQIGEFAYDYVEIRRNYDSKQF
DEPIIYEQRVEEPEHPPEEIEEAEEHSSTTKTNPPQEKASA"
gene complement(<389005..>390252)
/gene="csn2"
/locus_tag="AWJ20_3830"
/db_xref="GeneID:30035895"
mRNA complement(<389005..>390252)
/gene="csn2"
/locus_tag="AWJ20_3830"
/product="COP9/signalosome complex subunit Csn2"
/transcript_id="XM_018880863.1"
/db_xref="GeneID:30035895"
CDS complement(389005..390252)
/gene="csn2"
/locus_tag="AWJ20_3830"
/inference="similar to AA sequence:KEGG_Orthology:K12176"
/codon_start=1
/product="COP9/signalosome complex subunit Csn2"
/protein_id="XP_018733511.1"
/db_xref="GeneID:30035895"
/translation="MTSPVITKNYAEKSLNNIIERITCDDLDFMDKIYTTTLDKLKAK
QENDRLWIRTSLKLANLYISRELVDKAQDLVNELMEVCHQTNSGSDTIMSTYLLEIYA
LEIQVCMLTGNIKRLKELYSKTLNVTTAVLHPRILGVIRECGGKMHMQEKKWDIARED
FFESFKNYDEAGSLHRTQVLKYYVLACMLSESGINPFESQETKPYKDDPQVSVIVQLV
DVFQKSDVEGFNELMKTHRAEIMSDSFFANFLDDIVLSIRSLGVLNLVKPYRRVSLDH
IQNILGVDQATLVEIIVGLILDDRLPNTKIDLTNGFIDLNITNQSASVSSSKQDTRFS
TSTSSNNVAPLPSRYYESDRDSAQQQEQQNLVRASSDAVAMAGLSSTSNTSTTTSHTD
SQRPVSLAHWLGSLERLHSAIHA"
gene complement(<391496..>394201)
/gene="NAM2"
/locus_tag="AWJ20_3831"
/db_xref="GeneID:30035896"
mRNA complement(<391496..>394201)
/gene="NAM2"
/locus_tag="AWJ20_3831"
/product="leucine--tRNA ligase NAM2"
/transcript_id="XM_018880864.1"
/db_xref="GeneID:30035896"
CDS complement(391496..394201)
/gene="NAM2"
/locus_tag="AWJ20_3831"
/inference="similar to AA sequence:KEGG_Orthology:K01869"
/note="Mitochondrial leucyl-tRNA synthetase; also has a
direct role in splicing of several mitochondrial group I
introns; indirectly required for mitochondrial genome
maintenance; GO_component: GO:0005759 - mitochondrial
matrix [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 1990003]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0002161 -
aminoacyl-tRNA editing activity [Evidence IEA];
GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
[Evidence IEA,IEA]; GO_function: GO:0004823 - leucine-tRNA
ligase activity [Evidence IEA,IEA]; GO_function:
GO:0004823 - leucine-tRNA ligase activity [Evidence IDA]
[PMID 1990003]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0003729 - mRNA binding
[Evidence IPI] [PMID 11142386]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_process:
GO:0000372 - Group I intron splicing [Evidence IMP] [PMID
12486008]; GO_process: GO:0000372 - Group I intron
splicing [Evidence IMP] [PMID 2277640]; GO_process:
GO:0006429 - leucyl-tRNA aminoacylation [Evidence IEA];
GO_process: GO:0006429 - leucyl-tRNA aminoacylation
[Evidence IDA] [PMID 1990003]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IGI] [PMID 16777356];
GO_process: GO:0006450 - regulation of translational
fidelity [Evidence IEA]; GO_process: GO:0006418 - tRNA
aminoacylation for protein translation [Evidence IEA];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="leucine--tRNA ligase NAM2"
/protein_id="XP_018733512.1"
/db_xref="GeneID:30035896"
/translation="MFRVIQQYPRLVPGCALNYGARFYSESAGLLSTQLDEKWKKIWK
SHPPRADAATSDTSKGKYFVLSMFPYPSGILHIGHLRVYTISDVLARYRRLSGYDVIH
PMGWDAFGLPAENAAIERNVDPAVWTYSNIEKMRAQMDIMLADLDWDKEVITCSPEYY
KFTQKLFIMLYESGYAYRKEAVVNWDPIEKTVLANEQVDNEGRSWRSGAIVEKKKLEQ
WFLAITKLAPQLLDDLKVLDKWPKKVKTMQRNWIGKSEGAEIQFLFNPSSENGLISKL
GLDRVSAFTTRPDTLFGVDYVALSLNHPVSEWYSKQHPQLAKFLEEAKNLPEDSKAGF
KIPGLFLSNPLTPDTYDIPVFVAPYVIGDYGHGAVMGCPGHDLRDNAFWAENNPGVEP
SVVVRPASDTVAPGEIFAGKDGILNEKSGQFAGLTSKEGGSAIIDYVSKLNGLASKTT
QLKLRDWLISRQRFWGAPIPMIHCETDCGIVPVPEEDLPVLLPDGLGAPLSTSEEFTK
TTCPKCGGPAHRDTDTMDTFMDSSWYFFRYTDPKNPNQLFDYEKASTLMPVDLYIGGV
EHAILHLLYSRFISKFLAETGAWSGGDLNGEPIKRLVTQGMVHGRTLIDGETGRFLKP
EEVDESNPQVGPIIKSTGKVAEISSEKMSKSKFNGADPVKCITSHGADATRAHILFQA
PVSDVLNWEEQRIVGIERWLARVQSAVDSTIEKGLDTTSELDVSKLSAEDVKLWNEVQ
KKIEQITAALHEDLSMNTLISDYMKLTRTILNGLESPTTSPAVLYKSTKTLLQLISPV
TPANAEEGWEKLLKADGKEWTTIFNQRWPQPSPIEVTIKASGKHRIIINGKSVFELEI
TDTSISDDELIALIRSNGGDEHLAGKTIKRVIRPDGKPAVSLIAA"
gene <396173..>397399
/gene="NHX1"
/locus_tag="AWJ20_3832"
/db_xref="GeneID:30035897"
mRNA <396173..>397399
/gene="NHX1"
/locus_tag="AWJ20_3832"
/product="bifunctional K:H/Na:H antiporter NHX1"
/transcript_id="XM_018880865.1"
/db_xref="GeneID:30035897"
CDS 396173..397399
/gene="NHX1"
/locus_tag="AWJ20_3832"
/inference="similar to AA sequence:KEGG_Orthology:K12041"
/note="Na+/H+ and K+/H+ exchanger; required for
intracellular sequestration of Na+ and K+; located in the
vacuole and late endosome compartments; required for
osmotolerance to acute hypertonic shock and for vacuolar
fusion; ortholog of human NHE9, which is linked to autism;
GO_component: GO:0005769 - early endosome [Evidence IDA]
[PMID 22210050]; GO_component: GO:0005768 - endosome
[Evidence IEA]; GO_component: GO:0010008 - endosome
membrane [Evidence IEA]; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
20826459]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0005770 - late endosome
[Evidence IDA] [PMID 20709757]; GO_component: GO:0005770 -
late endosome [Evidence IDA] [PMID 22210050];
GO_component: GO:0005770 - late endosome [Evidence IDA]
[PMID 9694857]; GO_component: GO:0031902 - late endosome
membrane [Evidence IEA]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005802 -
trans-Golgi network [Evidence IDA] [PMID 22210050];
GO_function: GO:0015297 - antiporter activity [Evidence
IEA]; GO_function: GO:0015386 - potassium:proton
antiporter activity [Evidence IMP] [PMID 15635088];
GO_function: GO:0015385 - sodium:proton antiporter
activity [Evidence IEA]; GO_function: GO:0015385 -
sodium:proton antiporter activity [Evidence IMP] [PMID
10998367]; GO_function: GO:0015385 - sodium:proton
antiporter activity [Evidence IMP] [PMID 15635088];
GO_function: GO:0015299 - solute:proton antiporter
activity [Evidence IEA]; GO_process: GO:0006812 - cation
transport [Evidence IEA]; GO_process: GO:0030004 -
cellular monovalent inorganic cation homeostasis [Evidence
IMP] [PMID 10589731]; GO_process: GO:1902600 - hydrogen
ion transmembrane transport [Evidence IEA]; GO_process:
GO:0006811 - ion transport [Evidence IEA]; GO_process:
GO:0071805 - potassium ion transmembrane transport
[Evidence IMP] [PMID 15635088]; GO_process: GO:0006813 -
potassium ion transport [Evidence IEA]; GO_process:
GO:0006885 - regulation of pH [Evidence IEA]; GO_process:
GO:0035725 - sodium ion transmembrane transport [Evidence
IMP] [PMID 10998367]; GO_process: GO:0035725 - sodium ion
transmembrane transport [Evidence IMP] [PMID 15635088];
GO_process: GO:0006814 - sodium ion transport [Evidence
IEA,IEA]; GO_process: GO:0055085 - transmembrane transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]; GO_process: GO:0007035 - vacuolar
acidification [Evidence IMP] [PMID 10589731]"
/codon_start=1
/product="bifunctional K:H/Na:H antiporter NHX1"
/protein_id="XP_018733513.1"
/db_xref="GeneID:30035897"
/translation="MTALVLKHSHVRRFPQIESCLVLLFAYASYFFSNGCHMSGIVSL
LFCGITLKHYAYYNMSRRTQIAIKYIFQLLAQLSENFIFVYLGLSLFTQEELIFKPVL
IIVTALGICVSRWAAVFPLSKLINFASRARATRILRGSSLGSASGASASLTDEIPQPY
QMMLFWAGLRGAVGVALAAGLEGEHGPSLRATVLVIVVLTVIVFGGTTARMLEILGIR
TGVIEDVESDDEFDIEAVLDSRHQREEGFGYNSRSGNLSATHLIGGGVGSTSSPGDLG
NISGGFYQPGSRSGSVTRLRRSGSYHDDQLDAANIHGNNNEFDSDVDSDVSDLPPMAG
SRSAAGSSSVALDNLGPLNSSNGGSGASGDLAANSSGTSFLRAEDRARWFREFDEQVL
KPVLLDSSPGANSPNR"
gene <399759..>402416
/gene="SMC6"
/locus_tag="AWJ20_3833"
/db_xref="GeneID:30035898"
mRNA <399759..>402416
/gene="SMC6"
/locus_tag="AWJ20_3833"
/product="DNA repair protein SMC6"
/transcript_id="XM_018880866.1"
/db_xref="GeneID:30035898"
CDS 399759..402416
/gene="SMC6"
/locus_tag="AWJ20_3833"
/note="Component of the SMC5-SMC6 complex; this complex
plays a key role in the removal of X-shaped DNA structures
that arise between sister chromatids during DNA
replication and repair; homologous to S. pombe rad18;
GO_component: GO:0030915 - Smc5-Smc6 complex [Evidence
IEA]; GO_component: GO:0030915 - Smc5-Smc6 complex
[Evidence IDA] [PMID 15738391]; GO_component: GO:0005694 -
chromosome [Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 11927594]; GO_component: GO:0035861 -
site of double-strand break [Evidence IDA] [PMID
16892052]; GO_function: GO:0005524 - ATP binding [Evidence
IEA]; GO_function: GO:0019789 - SUMO ligase activity
[Evidence IDA] [PMID 15738391]; GO_function: GO:0003684 -
damaged DNA binding [Evidence IDA] [PMID 16793545];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_process: GO:0006310 - DNA recombination [Evidence
IEA]; GO_process: GO:0006281 - DNA repair [Evidence
IEA,IEA]; GO_process: GO:0006281 - DNA repair [Evidence
IDA] [PMID 11927594]; GO_process: GO:0006281 - DNA repair
[Evidence IMP] [PMID 15738391]; GO_process: GO:0006974 -
cellular response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0051304 - chromosome separation [Evidence
IMP] [PMID 15793567]; GO_process: GO:0000724 -
double-strand break repair via homologous recombination
[Evidence IEA]; GO_process: GO:0000724 - double-strand
break repair via homologous recombination [Evidence IMP]
[PMID 16892052]; GO_process: GO:0071139 - resolution of
recombination intermediates [Evidence IMP] [PMID
20159973]"
/codon_start=1
/product="DNA repair protein SMC6"
/protein_id="XP_018733514.1"
/db_xref="GeneID:30035898"
/translation="MDTRQEPVKGDESLTVSPPPAKRFRSQRFNDDNDNSSSPSPVDS
ESLTSSFTSPEDANNDGAGPSNLSQDLDLDQYEGERDQTISPTIGIDGTPEPEQSVEI
QRGSREMEGIARSPQSQSAGKIISVQMINFMCHSNLTISLGRRINFIVGKNGSGKSAV
LTAIIIGLGGRASDTDRGSSISDVIKEGQQMASIRIKFDNVGKLSFRHEIYGDFFTLE
RAISRTERAGHSNYNVYTQSGKKMKISRTDILEMLSISHFSIDIDNQFAILTQDQART
FLVARTQEEMYHLLMRGLRVTQSYHNANVSNNNITATESDLKAKRTDIKELKEKVKNL
ELRRNNNLRIEEAMREVARYTAMGLWFRHDRQMEKISQLEERNDELKREQAEKQDEAD
AYSKRAQELVVDTDSQVSELRDQRDKSSAERLEAINRRRELSDKLDSLKQNQVNVNTE
KNKNEQHKVELESELNRLTTSEGGKEYLEAQIETVNLKINELKEEEMALVNEAENFES
KIKEASDLVKSLQGEVKKYESSILSEKSQLAKIQEGSQVSVDAAYGVNMANVLKEISM
NSSFHQKPIGPLGQFVSLKIPTWGPMLEHHLRHTLAAFWTKNTEDRNLLLKILAKYKM
TNTVIVKDVDMFDYSWSSPSKEYTTVLDALEFKDESVKRIFIDQYQIHRILLIEDRAE
ADRVMYSHPKNALVAFAKQKSIDGSQSRDGFQVGANRGGSSSSILRGAPVNKTPRMGV
DLRYRVKELEDSIRSTQSQCTRVRSKLDDATRALGESKESSRQTTRRLSSLRHEMRTL
DEKRDDLQTQLEDYDNGKVLEVQGEIENCKQRIETYILQFEKIHADMLETQEDVRLAD
TIIAEADKIHDELCNRIETIRVCIAVLIL"
gene <402676..>403341
/gene="SMC6"
/locus_tag="AWJ20_3834"
/db_xref="GeneID:30035899"
mRNA <402676..>403341
/gene="SMC6"
/locus_tag="AWJ20_3834"
/product="DNA repair protein SMC6"
/transcript_id="XM_018880867.1"
/db_xref="GeneID:30035899"
CDS 402676..403341
/gene="SMC6"
/locus_tag="AWJ20_3834"
/note="Component of the SMC5-SMC6 complex; this complex
plays a key role in the removal of X-shaped DNA structures
that arise between sister chromatids during DNA
replication and repair; homologous to S. pombe rad18;
GO_component: GO:0030915 - Smc5-Smc6 complex [Evidence
IEA]; GO_component: GO:0030915 - Smc5-Smc6 complex
[Evidence IDA] [PMID 15738391]; GO_component: GO:0005694 -
chromosome [Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 11927594]; GO_component: GO:0035861 -
site of double-strand break [Evidence IDA] [PMID
16892052]; GO_function: GO:0005524 - ATP binding [Evidence
IEA]; GO_function: GO:0019789 - SUMO ligase activity
[Evidence IDA] [PMID 15738391]; GO_function: GO:0003684 -
damaged DNA binding [Evidence IDA] [PMID 16793545];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_process: GO:0006310 - DNA recombination [Evidence
IEA]; GO_process: GO:0006281 - DNA repair [Evidence
IEA,IEA]; GO_process: GO:0006281 - DNA repair [Evidence
IDA] [PMID 11927594]; GO_process: GO:0006281 - DNA repair
[Evidence IMP] [PMID 15738391]; GO_process: GO:0006974 -
cellular response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0051304 - chromosome separation [Evidence
IMP] [PMID 15793567]; GO_process: GO:0000724 -
double-strand break repair via homologous recombination
[Evidence IEA]; GO_process: GO:0000724 - double-strand
break repair via homologous recombination [Evidence IMP]
[PMID 16892052]; GO_process: GO:0071139 - resolution of
recombination intermediates [Evidence IMP] [PMID
20159973]"
/codon_start=1
/product="DNA repair protein SMC6"
/protein_id="XP_018733515.1"
/db_xref="GeneID:30035899"
/translation="MEKDMTIENLAQKILELRATMETLEKENGCDMVELVKQYTEANT
ALLNAKKEYDNLNRVYSVLIGSSKNRESKYEAIVTATLDSIKKGFKVNLGMRGFNGKL
IIDRTEENLIMDIDPVKSAVTRKVETLSGGEKSFAQIALLISIWRAMPSKLLALDEFD
VFMDNANRKQSLDMVIKSLGNRYNAQSIVITPQDMEIDDVQLKNNDITIQRMPDPVRS
APH"
gene complement(<403435..>405690)
/gene="USO1"
/locus_tag="AWJ20_3835"
/db_xref="GeneID:30035900"
mRNA complement(<403435..>405690)
/gene="USO1"
/locus_tag="AWJ20_3835"
/product="Uso1p"
/transcript_id="XM_018880868.1"
/db_xref="GeneID:30035900"
CDS complement(403435..405690)
/gene="USO1"
/locus_tag="AWJ20_3835"
/note="Essential protein involved in vesicle-mediated ER
to Golgi transport; binds membranes and functions during
vesicle docking to the Golgi; required for assembly of the
ER-to-Golgi SNARE complex; GO_component: GO:0012507 - ER
to Golgi transport vesicle membrane [Evidence IDA] [PMID
17192843]; GO_component: GO:0005794 - Golgi apparatus
[Evidence IEA]; GO_component: GO:0000139 - Golgi membrane
[Evidence IEA,IEA]; GO_component: GO:0000139 - Golgi
membrane [Evidence IDA] [PMID 17192843]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0031410 - cytoplasmic vesicle [Evidence IEA];
GO_component: GO:0030659 - cytoplasmic vesicle membrane
[Evidence IEA]; GO_component: GO:0005856 - cytoskeleton
[Evidence IEA,IEA]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0008565
- protein transporter activity [Evidence IEA]; GO_process:
GO:0006888 - ER to Golgi vesicle-mediated transport
[Evidence IMP] [PMID 8603910]; GO_process: GO:0048211 -
Golgi vesicle docking [Evidence IDA] [PMID 9545229];
GO_process: GO:0048193 - Golgi vesicle transport [Evidence
IEA]; GO_process: GO:0035493 - SNARE complex assembly
[Evidence IMP] [PMID 8603910]; GO_process: GO:0006886 -
intracellular protein transport [Evidence IEA];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA];
GO_process: GO:0048280 - vesicle fusion with Golgi
apparatus [Evidence IEA]"
/codon_start=1
/product="Uso1p"
/protein_id="XP_018733516.1"
/db_xref="GeneID:30035900"
/translation="MFRTELHKLLSSTIGRDQYVVKFKKLRENPVLKEFDVDDIFSYE
RDKTGLPQVYLDSAYVDLLKENSARIQKALDRDPTVEPNSRVSLDMYESLQSEHLDIC
RELEELKEKTTGLEHSTSEQIENLKETISKTTGLLQESEKNLKAAKSEKESLELAHTK
SIKDMEELEVKYNKLDENFKQISDSLQKANRAVEEHKQKEKQLISQLGNMTRDKEKAE
GGINKMNRELFSLTRAKEDADKENKTLQKQIQDLQKQILKSQESDSKLKSQLKEMTAS
RDKILRESKESLEAAKKFELQVKASSAELVQVKTQFQELSDSFTEVETLKETLISKLQ
SAAQQIEQLKSRKAELGTEIEKLNNEKANLSSELDKSKLLNSELKSKVDAIALDKDSR
GKEIESLSRRIVEFETAKSKLEDELKISENTTEALTKEKQESELLKTALREEINQLKI
ELDELRAENNTLKLEAANLVENGNPKQAEELEKKYKDQVSEMEFKLKNLSDELSAAKQ
KDSEDKDTQLSTLEDEISSLEKEKSILEKVNSAKLASLEKAHSTEKDSLEQQHVKEKD
NLHKDFSDQKSILEKEFVKQKEEILSSHENEKETLEKAHRKAIEENKKILFASHTDAM
EKLRNELQKSQSAEAEKLKDLYEKSQQENIALSRKFEEQVAKLESENKNLTQKLQLGE
KSKDKPTVDMVPKSDLDDLMLLLNDLDEQKKKYKKQLLKLGADVTDSEDDSEDDDDEE
EGDDEGDEDGN"
gene complement(<405732..>407753)
/gene="USO1"
/locus_tag="AWJ20_3836"
/db_xref="GeneID:30035901"
mRNA complement(<405732..>407753)
/gene="USO1"
/locus_tag="AWJ20_3836"
/product="Uso1p"
/transcript_id="XM_018880869.1"
/db_xref="GeneID:30035901"
CDS complement(405732..407753)
/gene="USO1"
/locus_tag="AWJ20_3836"
/note="Essential protein involved in vesicle-mediated ER
to Golgi transport; binds membranes and functions during
vesicle docking to the Golgi; required for assembly of the
ER-to-Golgi SNARE complex; GO_component: GO:0012507 - ER
to Golgi transport vesicle membrane [Evidence IDA] [PMID
17192843]; GO_component: GO:0005794 - Golgi apparatus
[Evidence IEA]; GO_component: GO:0000139 - Golgi membrane
[Evidence IEA,IEA]; GO_component: GO:0000139 - Golgi
membrane [Evidence IDA] [PMID 17192843]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0031410 - cytoplasmic vesicle [Evidence IEA];
GO_component: GO:0030659 - cytoplasmic vesicle membrane
[Evidence IEA]; GO_component: GO:0005856 - cytoskeleton
[Evidence IEA,IEA]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0008565
- protein transporter activity [Evidence IEA]; GO_process:
GO:0006888 - ER to Golgi vesicle-mediated transport
[Evidence IMP] [PMID 8603910]; GO_process: GO:0048211 -
Golgi vesicle docking [Evidence IDA] [PMID 9545229];
GO_process: GO:0048193 - Golgi vesicle transport [Evidence
IEA]; GO_process: GO:0035493 - SNARE complex assembly
[Evidence IMP] [PMID 8603910]; GO_process: GO:0006886 -
intracellular protein transport [Evidence IEA];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA];
GO_process: GO:0048280 - vesicle fusion with Golgi
apparatus [Evidence IEA]"
/codon_start=1
/product="Uso1p"
/protein_id="XP_018733517.1"
/db_xref="GeneID:30035901"
/translation="MLSGFLSQTQPKPQTASVTITKLCDRLQHSTLLEDRRAAVLGLK
GFSREYRETVAAGGLRGLISALHRDSEDAETCRATLETLLILFMPDELSEDQATTTNG
NSTGSGRQARRPLEQNSRFTRSRGKKYSSPLLKPNHNPDNISLWLTDEFTQKQDNIIT
LLELVEKTDDFFTKLYALQLLSAIISNRPLRSQECILAAPLGISSLVACLDDYRDVIR
NEAILLLLYLVSEHFDMQKLVAFEGTFDKLFSIIDIEGGLNGGVVVEEAISLLSALLR
YNVSNQHHFRETNCVPKLVSLLENKDTLDQLSSDDSGSLSNAIIGTLELCRLFVIEDH
WSTPVNQQALCKEGLLFTVLRLAFGVSTPMPVRCSAFLTAAALVRGNSPLQEEFLAID
VPYFDLSIAFNVEKEPLIIPVSLALLNWVLLSTSVHTFDLRVAAQLCLSSALQGNIQA
KLSFLKDQMASYPGNMSEVADELEIKDKVESRSTSTSPNLEQIETNAPKIVGDAKPQP
DYPEGSANLFSALVDYDPDARLNPYKPWFAAVILINLFEDADISNPSHYNTESHEVRD
FVRGLMIGDASIGEEVVSCIQAMSGMLVTSLRYPDPRISLGYLMLLSIWLYDDTKAVS
DFLGESGTIQALISSATHSSNNDPLVEALSTVLLGIVYDFSSAESPIPR"
gene <412571..>414010
/locus_tag="AWJ20_3837"
/db_xref="GeneID:30035902"
mRNA <412571..>414010
/locus_tag="AWJ20_3837"
/product="hypothetical protein"
/transcript_id="XM_018880870.1"
/db_xref="GeneID:30035902"
CDS 412571..414010
/locus_tag="AWJ20_3837"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733518.1"
/db_xref="GeneID:30035902"
/translation="MTEGSPGLLQDQKYDTSRNIIILDTSHYAQSAMLSSDPPVTDHE
NGHALPTNGSTIYTSESYQSRPQQPAPDNPPERPPQGELIIRDRINPFATVPGTLLPP
ECTVKHQDLELIHHYSFHTDNIIMMQSAREKPIWERVVPKVSFAFPYLSDALLAISAL
HLQHCSDGSRDLRNYALQKYYNAMSSSQVLDITNSFEPKFLTSCFLMMLAFSFDDVID
LISFNGKPDVFALSRGPYILAERLFPFLSKGPLRGLLRQVPGEDNAFAKAMELRLPAY
ENLYSVLSMLSRPSVSRSSQSSSDSSHRYSIPEDAIPMANFDFVEVKSEVETSSSNSS
VTTSEGNFPTSVVDTYASHMHEHSILKNELDRLICIVKASIYFNAEPLIITWITSVSN
DFANLARRNNSFAKVIIAYYMAALHTCQGVFWIGGRPAREVIKIANSLPSEWTDLMSK
PLQIVQRQYVSLTDLLELSGWVSDTPGHL"
gene complement(<414180..>420110)
/gene="UTP20"
/locus_tag="AWJ20_3838"
/db_xref="GeneID:30035903"
mRNA complement(<414180..>420110)
/gene="UTP20"
/locus_tag="AWJ20_3838"
/product="Utp20p"
/transcript_id="XM_018880871.1"
/db_xref="GeneID:30035903"
CDS complement(414180..420110)
/gene="UTP20"
/locus_tag="AWJ20_3838"
/inference="similar to AA sequence:KEGG_Orthology:K14772"
/note="Component of the small-subunit (SSU) processome;
SSU processome is involved in the biogenesis of the 18S
rRNA; GO_component: GO:0030686 - 90S preribosome [Evidence
IDA] [PMID 12150911]; GO_component: GO:0030686 - 90S
preribosome [Evidence IDA] [PMID 17652137]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
17652137]; GO_component: GO:0005730 - nucleolus [Evidence
IEA]; GO_component: GO:0005730 - nucleolus [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 15590835]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 17652137]; GO_component:
GO:0005654 - nucleoplasm [Evidence IDA] [PMID 17652137];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0030688 - preribosome, small subunit
precursor [Evidence IDA] [PMID 17652137]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0032040 - small-subunit processome
[Evidence IDA] [PMID 15590835]; GO_function: GO:0003729 -
mRNA binding [Evidence IDA] [PMID 23222640]; GO_process:
GO:0000480 - endonucleolytic cleavage in 5'-ETS of
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 15590835]; GO_process:
GO:0000480 - endonucleolytic cleavage in 5'-ETS of
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 17652137]; GO_process:
GO:0000447 - endonucleolytic cleavage in ITS1 to separate
SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic
rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 15590835]; GO_process: GO:0000447 -
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
17652137]; GO_process: GO:0000472 - endonucleolytic
cleavage to generate mature 5'-end of SSU-rRNA from
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15590835]; GO_process: GO:0000472 - endonucleolytic
cleavage to generate mature 5'-end of SSU-rRNA from
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
17652137]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA]"
/codon_start=1
/product="Utp20p"
/protein_id="XP_018733519.1"
/db_xref="GeneID:30035903"
/translation="MIINIVESLRSPSHDVRYLAIQVIIFIYQAMRLEVPKLITDCKL
IDEIPLDVSNARNFGLYIRNLSNSYAGTGADTLLDLIIPRFFFGLLTAKFQPIWEASV
EAISKISEKNGEIIWSLAYEWIVYEPKISPVFGMFDDIILDEPEQSATVECSNLSQVI
NHSVSSFYKYSNSQNFSKLSIRDSLDKIDFPPTVRGQIIKVLASIPRVAEKHSRLLVP
FLLWDINSDDGEDSEETETPLSSTWSVKDRIALLDLFSLFGNPKTLYKSEEVYERYLY
LLGNRSLAVQKIALKCLLTFKNPQIRKYRDNLEGMLDDTQFKDELTNLVRYSGEEGTI
HEDDRAALLPIVIRIIFGRAQIAKTGGVKQGRRFAVVNSLSNVEPEYLRLFIDLSANR
LEHEGFLKGSYDGVLTINPEFSHFQSSDLRLRRELGFVTMLEEILEQLGDRVHVAWDI
IIESLILCLYRANQAHTDLNEQDEDEPTDSVIGKNIRSIRQLGFKCLNALFSILPGSN
WAGCFDIIYSNFISNRMAKFAEENLQQPSSLMRIFVTLSSEKSLVRFLTWDNCAIIKS
FFACLSYDEVKDSVVDVIMDTMNNILELERSEDTETLSVTGVPIVLNRMPYLFERQSN
HQLLEKEAQLLVKLMTSGYEASDDIRKQLIEVSLAALEKNTTLVRVSVKVSVLQTLSV
LLMDGRSTLEECTSAYYTLSKLFRQFTNRQARENLAAVFDVFGDQFDVFKTVSKLVVD
LNSYSAKRLDLPDFDRRLSAFAVINETKFDKLTAMEWLPLLYNMLFFMKDPEELALRT
NATYSVKRFIDGLASRTNEEDVSSYVQLLDDVVIPVVKTSLRDSNDIFRTQFIDVLGH
IVKAAKWYSKMNDMKCLLFDGDEEANFFNNINHLQIHRRRRAVRRLSKLASKGSLTDT
NIAHYILPIIEHFVGGTEGDAHNLSEDSIKCIGVLARHLTWNQYRAITKRYIAYLNSR
PESIKATVKLVETASDALISPVDETDMDLSLLEEDEDEDTTMAGSEDISPAVVNSVEG
AVKLADCLPSVERLSAFITEDIVPTMQKVLNKKNDDDTLTLRIPLALPIVKFIKALPE
ALIELKLPGVLTGLCQILRAKSQELRDMIRSTLGRIARILGTKYLVFIIKELKGALRR
GAQLHILGYSLHSLLSELKDIILPRDLDVSATLIMDIIMEDTFGVTGGEKDEEGYTSD
MKEVKQYKSYDSGQILASNVGLGTFSELVEPVKTLLLYEKLNLKMEKKVEELLRRFAL
GLYHNIDASKQEVLVMCFELYEMVNNVSTEIEKKKKIDSESHIRDEQKREAEEHFMIQ
LDSRHWGEQNTIRIGNLHIITKFVIEMIRTVLGKNEHMMTAENVAGFMPMLDEGLNGN
YEDVQIASLRLLAHIIKLPIPDVSGRVVSLGRRTLWLIKSSTSTNTELCQACLRFISV
CIRQKKDFSLKEKELAYCLNRLKPDIEEPDRQGITLTFIRAVLSRQIMMPEVYDIMDQ
VAQVMVTNQSNVTREACRSAYFQFLTEYPQGKGRLTKQLKFLVANLEYPAVSGRLSVM
EIIHLLLAKVTDDQVQEVIASFFVALVLVLINDEESQCRETAAALIGELLDISKDEQL
SLIEKYCTTWLTNRENELLLRGGFQVTGIYCAKFGVRKNPKLFKLAISVLEKLLEDAK
SDSNVVVQWETLYQGLQFVAKTVQNSNGLKILFSSNYQQLWDLIESSLLFPHAWVRLA
SSRLIGKLYSVYSGGKNCEITISTDGLQSTAFKFVRQLGAASISEDLGIQIVKNLVFI
SMQWEKSEETIYFVKPRSSNEDEDEDDDTATEERIPVIRWLIQKISSVLRAEKNFKEM
IVSKKASIQLLASIIQLLSVEKLSEMAEDIIMAFYIYSISEEDRTRDLKELCLEALNM
VQKKLGTTKYVQAYSNVQQQVSERRLERKSKRAIQSVSAPDVYARKKLRKNLAKREKR
KHSKDENGYYHSKKSKNA"
gene complement(<420190..>421350)
/locus_tag="AWJ20_3839"
/db_xref="GeneID:30035904"
mRNA complement(<420190..>421350)
/locus_tag="AWJ20_3839"
/product="hypothetical protein"
/transcript_id="XM_018880872.1"
/db_xref="GeneID:30035904"
CDS complement(420190..421350)
/locus_tag="AWJ20_3839"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733520.1"
/db_xref="GeneID:30035904"
/translation="MSFLLRKSTGSSLDLIIDHILDDVISHPKADFVTSVANLFSDSI
KSSGNNLHSRTSEILSALLRSCKKHVNQSLVVVDVSSAVLVALLHHVRQETAHILYTE
SMTFVDSMLGEKELSDNQIILAQTVIRDLSGLRKGSRVSDWTPLFGKFLSILGRITEA
SSQQVLISTLTASVSLLQSANFESTTKYCSPLVQELYRLLGQEYFLSFCESMVEYNPT
VFSNHLIVYVQRFIKEYRSDVSSVHLLLTKLESAGLVNRTSQPVPGKLFTAPDSAFSK
SLEQKVQFPKLDSVNGLYDLFIALDIFAIAVIPTDKLSKSLPKLLERIILEVNESNIA
WKKALVGKTLSLVNEPSVAAEMIDTIKESFSELSDSKIFLEGFLNLWRANKG"
gene complement(<422530..>424269)
/gene="URA7"
/locus_tag="AWJ20_3840"
/db_xref="GeneID:30035906"
mRNA complement(<422530..>424269)
/gene="URA7"
/locus_tag="AWJ20_3840"
/product="CTP synthase URA7"
/transcript_id="XM_018880874.1"
/db_xref="GeneID:30035906"
CDS complement(422530..424269)
/gene="URA7"
/locus_tag="AWJ20_3840"
/inference="similar to AA sequence:KEGG_Orthology:K01937"
/note="Major CTP synthase isozyme (see also URA8);
catalyzes the ATP-dependent transfer of the amide nitrogen
from glutamine to UTP, forming CTP, the final step in de
novo biosynthesis of pyrimidines; involved in phospholipid
biosynthesis; capable of forming cytoplasmic filaments
termed cytoophidium, especially during conditions of
glucose depletion; URA7 has a paralog, URA8, that arose
from the whole genome duplication; GO_component:
GO:0097268 - cytoophidium [Evidence IDA] [PMID 20713603];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0005524 - ATP binding [Evidence
IEA]; GO_function: GO:0003883 - CTP synthase activity
[Evidence IEA,IEA]; GO_function: GO:0003883 - CTP synthase
activity [Evidence IMP,ISS] [PMID 1753946]; GO_function:
GO:0003883 - CTP synthase activity [Evidence IDA] [PMID
8075080]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0044210 - 'de novo'
CTP biosynthetic process [Evidence IEA]; GO_process:
GO:0006241 - CTP biosynthetic process [Evidence IDA] [PMID
8075080]; GO_process: GO:0006541 - glutamine metabolic
process [Evidence IEA]; GO_process: GO:0008654 -
phospholipid biosynthetic process [Evidence IMP] [PMID
9668079]; GO_process: GO:0019856 - pyrimidine nucleobase
biosynthetic process [Evidence IMP] [PMID 1753946];
GO_process: GO:0006221 - pyrimidine nucleotide
biosynthetic process [Evidence IEA,IEA]"
/codon_start=1
/product="CTP synthase URA7"
/protein_id="XP_018733521.1"
/db_xref="GeneID:30035906"
/translation="MKYVVVSGGVISGIGKGVIASSTGLLFKTMGLKATSIKIDPYMN
IDAGTMSPLEHGEVFVLNDGGEVDLDLGNYERYLNVTLTRDHNITTGKVYKQVIEKER
RGDYLGKTVQIVPHLTNAIQDWIEKVAHIPVDGDNEVPDVCIIELGGTVGDIESAPFV
EALRQFQFRVGPENFALIHVSLVPVIHGEKKTKPTQAAIKDLRSLGLTPDIIACRCSE
ELDQAAIDKISMFCHVGPNQVLGVHDVNSTYHVPLLLHDQKLMEFFSKRFKLDELKLS
SERIAKGQDLWTRWNSITQSHDRSFQKVTIALVGKYTNLQDSYISVVKALEHSALRCE
LKLEIVWVEASDLEIESSVTNKINYHKAWGSLCSADGILVPGGFGTRGTEGMIAAARW
ARENLVPYLGICLGFQVGVIDYARHVLNMKTNSIELDETIEDPVIVYMPEVDKINMGG
TMRLGIRPAVFQESTENSVLRRLYGSAPTVMERHRHRYEVNPEHIDELEKSGLHFVAK
DEKAERMEVFELKNHPYFVGTQYHPEYLSRVLDPSKPLLGLVAAAGGKLESILAATVD
HHSDQAKGPVTDF"
gene <423019..>423783
/locus_tag="AWJ20_3841"
/db_xref="GeneID:30035907"
mRNA <423019..>423783
/locus_tag="AWJ20_3841"
/product="hypothetical protein"
/transcript_id="XM_018880875.1"
/db_xref="GeneID:30035907"
CDS 423019..423783
/locus_tag="AWJ20_3841"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733522.1"
/db_xref="GeneID:30035907"
/translation="MTSIINDTNLETKTNTEVRNQIFTGPASSSNHTLSTSGTKSTRN
KNTISRAETAPGLVVVDLVSNGAFDLKIGSFDPNNLELKLASQSRVLKSLDNRNITVL
KIGIFTDQGNSDLLETTVMGLGNTIPTSPQILTLSNTLRRQFKFIELKTLGKEFHQLL
VVQKKRNMVSRIDIMDTQNLVRTDVTEHRDLVNSSLVKLFRTSTGNNIGCQTKGSQIL
DSSLSRFGLLLTMYNRNKRNVNQSKVLRTDSELKLT"
gene <425685..>426581
/gene="YPR1"
/locus_tag="AWJ20_3842"
/db_xref="GeneID:30035908"
mRNA <425685..>426581
/gene="YPR1"
/locus_tag="AWJ20_3842"
/product="trifunctional aldehyde reductase/carbonyl
reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1"
/transcript_id="XM_018880876.1"
/db_xref="GeneID:30035908"
CDS 425685..426581
/gene="YPR1"
/locus_tag="AWJ20_3842"
/inference="similar to AA sequence:KEGG_Orthology:K18097"
/note="NADPH-dependent aldo-keto reductase; reduces
multiple substrates including 2-methylbutyraldehyde and
D,L-glyceraldehyde, expression is induced by osmotic and
oxidative stress; functionally redundant with other
aldo-keto reductases; protein abundance increases in
response to DNA replication stress; YPR1 has a paralog,
GCY1, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0004032 -
alditol:NADP+ 1-oxidoreductase activity [Evidence IDA,ISS]
[PMID 11306085]; GO_function: GO:0004090 - carbonyl
reductase (NADPH) activity [Evidence IDA] [PMID 19016485];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_process: GO:0042843 - D-xylose
catabolic process [Evidence IDA] [PMID 12271459];
GO_process: GO:0019568 - arabinose catabolic process
[Evidence IDA,IMP] [PMID 12271459]; GO_process: GO:0034599
- cellular response to oxidative stress [Evidence IGI]
[PMID 17919749]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="trifunctional aldehyde reductase/carbonyl
reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1"
/protein_id="XP_018733523.1"
/db_xref="GeneID:30035908"
/translation="MSAATLNTSTATLNDGNKIPYVGFGTWQVNDETDGYDALIYALK
HGYRHIDTAFAYKNEAVVGKAVRDSGIPREEIFVVSKLWGKDHRDPAAALDKSLKLLG
LDYVDLYLMHWPVPFKNIEDKDEIDEDWNHVKTWELMQKLPKSKVKSIGVSNYDTALL
EELSKAPTTTVVPVINQVELHPYLAQDKLRDYCNEHKIVLEAYCPLGKGTKLLEEPIV
VELAEKYKVSPGQIALSWGIARGYVVIPKSSTPSRIEANLHTVKLSKEDEEKLTSLSK
TNPQRRVRPGWKTKILFNDDDF"
gene <427253..>428629
/gene="RMD1"
/locus_tag="AWJ20_3843"
/db_xref="GeneID:30035909"
mRNA <427253..>428629
/gene="RMD1"
/locus_tag="AWJ20_3843"
/product="Rmd1p"
/transcript_id="XM_018880877.1"
/db_xref="GeneID:30035909"
CDS 427253..428629
/gene="RMD1"
/locus_tag="AWJ20_3843"
/note="Cytoplasmic protein required for sporulation;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0008150 - biological_process
[Evidence ND]; GO_process: GO:0007126 - meiotic nuclear
division [Evidence IEA]; GO_process: GO:0030435 -
sporulation resulting in formation of a cellular spore
[Evidence IEA]"
/codon_start=1
/product="Rmd1p"
/protein_id="XP_018733524.1"
/db_xref="GeneID:30035909"
/translation="MSEQTPLLAPQTSAGSAMSRNQMPLGVAQGTTELMSQDIANQHH
LQQKGQSQEHQGSSGSTVTRTGPSSTNNQFHRHNNPNINTTPIRPDYPPVINGTGAGV
RRNGPQRTSYTTQKLKLLPDEPVSTDDDDISISEGDVYSQVARIKDRPARKDAERLGK
AHRSMLPRVTAYCTAGSYRINDLSRYFLGRKSHGTAPKLFDECLYTPYRYRRDNGSSS
TNTSPTLSTAPDPTSDETIVSSEADSQPSDFIRLDDEGGEIDVSYGRSELFLFDYGVT
VFWGFTEAEEKRFLKELARFETEKLADEDVQIEEFNYYITKSYQPRIYNDFITLRDNT
NYMIKLSISHAIAQSVKISLFEELVDNTIEDTQDFPQEIALTGKINMNRKNIMKSIGE
LFILRISKLKTVNEATKAIKYTNNQQILICTDQFWTRLNLCGQSHILSPFIKLPVDIL
KSTREFRS"
gene <434804..>436258
/locus_tag="AWJ20_3844"
/db_xref="GeneID:30035910"
mRNA <434804..>436258
/locus_tag="AWJ20_3844"
/product="hypothetical protein"
/transcript_id="XM_018880878.1"
/db_xref="GeneID:30035910"
CDS 434804..436258
/locus_tag="AWJ20_3844"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733525.1"
/db_xref="GeneID:30035910"
/translation="MKLTVGLVATLWVQLQLTQGLQPVIRDYSNPSTPASQFRLAYNG
AEGVTVSWNSPSQINSPQVWYGEDPNNLVASSPGTSTTFSTATSWDNHVTIGNLKPNT
KYYYRVTGLADNFDVVGQNFYFTTARTTGDRTPYSIGFIADLGIVIGNLFGKQIPDTF
DSILSARDSLDFLWHSGDFGYADDWLWEELTGAYPLNLDGGVATYNNIMNSYYDQFVN
VTKNTPYMVGPGNHEADCIEPDLDYITNKFDLTICPSGQRNFTYHRNHFAMPTAEQAQ
DYFQNLWYSWNHGMTHFVQINTETDLAAGTTAPDEPGGDGGLDAGPFGFPNQQLQWLE
ADLAAVDRTKTPWLVVAGHRPWYSASTVCTECQEAFENLVIKYKVDLVMAGHVHYYER
DAPVANGVVDPNGLNNPSAPWYITNGAAGNFEGHSSNSGKNPSYTSIINNSDYGWSKL
TFHNSTHLTHDFISSETDQVLDTATLYKDHGLGW"
gene complement(<437248..>439506)
/gene="CDC4"
/locus_tag="AWJ20_3845"
/db_xref="GeneID:30035911"
mRNA complement(<437248..>439506)
/gene="CDC4"
/locus_tag="AWJ20_3845"
/product="SCF ubiquitin ligase complex subunit CDC4"
/transcript_id="XM_018880879.1"
/db_xref="GeneID:30035911"
CDS complement(437248..439506)
/gene="CDC4"
/locus_tag="AWJ20_3845"
/inference="similar to AA sequence:KEGG_Orthology:K03361"
/note="F-box protein required for both the G1/S and G2/M
phase transitions; modular substrate specificity factor
which associates with core SCF (Cdc53p, Skp1p and
Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as
a ubiquitin-protein ligase directing ubiquitination of
cyclin-dependent kinase (CDK) phosphorylated substrates,
such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p;
GO_component: GO:0019005 - SCF ubiquitin ligase complex
[Evidence IDA,IPI] [PMID 9346238]; GO_component:
GO:0019005 - SCF ubiquitin ligase complex [Evidence
IDA,IPI] [PMID 9346239]; GO_component: GO:0043224 -
nuclear SCF ubiquitin ligase complex [Evidence IDA] [PMID
11080155]; GO_component: GO:0016363 - nuclear matrix
[Evidence IDA] [PMID 2244914]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 11080155]; GO_function:
GO:0050815 - phosphoserine binding [Evidence IDA] [PMID
23314252]; GO_function: GO:0043130 - ubiquitin binding
[Evidence IDA] [PMID 21070969]; GO_function: GO:0061630 -
ubiquitin protein ligase activity [Evidence IDA] [PMID
9346238]; GO_function: GO:0004842 - ubiquitin-protein
transferase activity [Evidence IDA] [PMID 9346239];
GO_process: GO:0000082 - G1/S transition of mitotic cell
cycle [Evidence IMP] [PMID 10409741]; GO_process:
GO:0000086 - G2/M transition of mitotic cell cycle
[Evidence IGI,IMP] [PMID 10409741]; GO_process: GO:0000086
- G2/M transition of mitotic cell cycle [Evidence IGI]
[PMID 7954792]; GO_process: GO:0031146 - SCF-dependent
proteasomal ubiquitin-dependent protein catabolic process
[Evidence IDA] [PMID 9346238]; GO_process: GO:0031146 -
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [Evidence IDA] [PMID 9346239];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0051301 - cell division [Evidence IEA];
GO_process: GO:0007126 - meiotic nuclear division
[Evidence IMP] [PMID 328339]; GO_process: GO:0007067 -
mitotic nuclear division [Evidence IEA]; GO_process:
GO:0016567 - protein ubiquitination [Evidence IEA];
GO_process: GO:0042787 - protein ubiquitination involved
in ubiquitin-dependent protein catabolic process [Evidence
IDA] [PMID 9346238]; GO_process: GO:0042787 - protein
ubiquitination involved in ubiquitin-dependent protein
catabolic process [Evidence IDA] [PMID 9346239];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="SCF ubiquitin ligase complex subunit CDC4"
/protein_id="XP_018733526.1"
/db_xref="GeneID:30035911"
/translation="MSEQDPLSLGHISRPAVAEQSFAAVMSGSNGSAGSGSVNEPSGH
SSIASSSSSAIIDHFNEEFKSPLTPAPSPTAHHHYSYSNSSSPNTSSHSPNSRNFHQL
QQEWNLDESQHYNYHLHQLRSRRTGGGRLDSVVGQDFPSAHDDYQKESSTSKALSLVE
SVRSQFGSLDDEGRSLFLGELLAMCGRAVLSETLDYISPMLKRDPMVVLPSELALKVL
SYVDDPLSLARASQVSKTWYLMISDDLTWKELCKTHHFRRLSAAVNVAMSGDNLRGRV
PSVVGVNSGIHPGTVVPGSSPTGSVSGQSDLTEAFYNPNATKRPVPTSYRSHFKQQYL
LNSAWASGGRLAAKYVISIPGVVTSLLMEGQYIVIALDNSKIFVFREDGRLLRALFGH
VMGVWALALRGHTLVSGGCDRDVRVWDLRSGGCSQILKGHSSTVRCLQMVDEDTAISG
SRDATIRIWDVKKGICKRVLEGHDASVRCVEVHGDICVSGSYDFTAKVWRISDGTLLH
TLVGHISQIYSLAFDGKRIATGSLDASIRIWDPETGLCLAFLQGHTSLVGQLQMKGNI
LVSGGSDGAIRVWDLENYTCLQRFTAHDNSVTTLQFDEERIVSGGSDGKVEVWELATG
KHIRSLSGPFDAVWRVAFKDEKIAILASRQNMIHMELMSFTPTVDRADSTATPPTIEF
RSTDGYGRRRGHSTKPSDDPGMSPTMVPVGRESPEHTMTDRPSGTVVTAPTAAATPGQ
PRQDDDAMDISA"
gene <442775..>443782
/locus_tag="AWJ20_3846"
/db_xref="GeneID:30035912"
mRNA <442775..>443782
/locus_tag="AWJ20_3846"
/product="hypothetical protein"
/transcript_id="XM_018880880.1"
/db_xref="GeneID:30035912"
CDS 442775..443782
/locus_tag="AWJ20_3846"
/inference="similar to AA sequence:KEGG_Orthology:K11205"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733527.1"
/db_xref="GeneID:30035912"
/translation="MSLIFYTGNTLDQTLLNDQKLSGRIINSIQAWRSNRSYSDPLKG
SRNSTANGNSSGTAATIQTTAAVTSSTTTASTSTTTTDSSTTITSSTTNSTSTNTTAV
IVPPISFHKPTAGCPNADLTAKLFLPANATPNQAHIAINEMLDIIGPLIPAGSVDGDA
LIKIFIISLEGLSFSADSDEEQNGNSASSSVSPSVEKIADIWKQVVSAVQTRYPKLVE
KYGIAELSTEKLTKLLAHLEAESAVLPSVDHINAADCCALPPGLISIAKQRGIRLLAH
HDPEEMLSQDALNRVLEAYNPELSSSSYRWNWILRLTYIAHDRQVLAGNDYLVSVSPS
E"
gene <447425..>448225
/gene="BXI1"
/locus_tag="AWJ20_3847"
/db_xref="GeneID:30035913"
mRNA <447425..>448225
/gene="BXI1"
/locus_tag="AWJ20_3847"
/product="Bxi1p"
/transcript_id="XM_018880881.1"
/db_xref="GeneID:30035913"
CDS 447425..448225
/gene="BXI1"
/locus_tag="AWJ20_3847"
/inference="similar to AA sequence:KEGG_Orthology:K06890"
/note="Protein involved in apoptosis; variously described
as containing a BCL-2 homology (BH3) domain or as a member
of the BAX inhibitor family; reported to promote apoptosis
under some conditions and to inhibit it in others;
localizes to ER and vacuole; may link the unfolded protein
response to apoptosis via regulation of calcium-mediated
signaling; translocates to mitochondria under
apoptosis-inducing conditions in a process involving Mir1p
and Cor1p; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IDA] [PMID 21673967];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IEA]; GO_component: GO:0000324 - fungal-type
vacuole [Evidence IDA] [PMID 14562095]; GO_component:
GO:0000324 - fungal-type vacuole [Evidence IDA] [PMID
21673659]; GO_component: GO:0000324 - fungal-type vacuole
[Evidence IDA] [PMID 21673967]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0031966 -
mitochondrial membrane [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 21673659];
GO_component: GO:0005774 - vacuolar membrane [Evidence
IEA]; GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0006915 - apoptotic process [Evidence
IEA]; GO_process: GO:0006915 - apoptotic process [Evidence
IMP] [PMID 21673659]; GO_process: GO:0006915 - apoptotic
process [Evidence IMP] [PMID 21673967]; GO_process:
GO:0019722 - calcium-mediated signaling [Evidence IMP]
[PMID 21673967]; GO_process: GO:0030968 - endoplasmic
reticulum unfolded protein response [Evidence IMP] [PMID
21673967]"
/codon_start=1
/product="Bxi1p"
/protein_id="XP_018733528.1"
/db_xref="GeneID:30035913"
/translation="MSTPSAPPPQYTAPGNGAQSSEPLLVPGEAPPRRQEDDNIPDDF
KYSTSVAECTLPIRHAFLRKVYTILFGQLVVTAAVGAVISQNSSVSHWVLTHIWTFYV
AIFGAMGLMIGAYVKQRSYPTNMLFLGGFTLLESYCVGIISSLYDTKIVIQAVVLTLV
IFGGLSLFALQTKYDFSGWQSYLGAALWGLIGFGLISIFMPYSSGVELAYSVVGALVF
AGYIVVDTQLIMRRYHPEDEVAAAIALYLDIINLFLNILRILNEMNRD"
gene complement(<448477..>450570)
/gene="PHO11"
/locus_tag="AWJ20_3848"
/db_xref="GeneID:30035914"
mRNA complement(<448477..>450570)
/gene="PHO11"
/locus_tag="AWJ20_3848"
/product="acid phosphatase PHO11"
/transcript_id="XM_018880882.1"
/db_xref="GeneID:30035914"
CDS complement(448477..450570)
/gene="PHO11"
/locus_tag="AWJ20_3848"
/inference="similar to AA sequence:KEGG_Orthology:K01078"
/codon_start=1
/product="acid phosphatase PHO11"
/protein_id="XP_018733529.1"
/db_xref="GeneID:30035914"
/translation="MTDHQSIENEEQSAFLARSSLDDGLDLDHELDHEFELDPSSTGS
PYDEESSIETGDNGIAKVGSRNNASSLDTTGSSGASITKGRKPKVNFDLDETYVQYDE
AYKGIKGRSKTTTNSKGNISRHFDYSSSVNPIPPSPTSHRRGNNRLSSRKSSKLTTKL
IIMLVLPALVLFILFFAVSSSASNPPRNASPVVAPTGLTYPSGFNMKQNWGSASPYFD
TGVSYTGIDSTKAFERSARCSLRQSHVLHRHAERYPTDGMTARLSATAEKLMKMPPPP
SKQFEWISKWNYTLGADLLTARGVGTEFKSGSDYWGSHGVALYNATSNGNNELFYSPK
LNVYENGTSRPVPVLRATTQSRIETSARAWAAGFFGVYGGQIYNVVENSHDGSKNNNG
DGTGWNSPVKPDSPVNYVSDAGIYKLVLQAENHGNNNTLASYYACPNDNSDNIGKEKK
YAWIDNYLAPAAVRLNKVFPDYGNMTAGEVYDLQNFCAFETAAYGSSRFCELFTESEW
RGFEYAGDLSFYYDSSFGSPVGASMGAGYLVELLARLQNKSVIAELNKGVNLTLDNDP
ATFPLGQPFYLDMTHDSIIISVLTAIGFDFINKELPTDKLLAPRQFVASRLVPFGARL
IFDILECDDSTSSDSPPDLNYYVRVSLNSRVLPLGSLHDCPQSEDGFCPLDKFASSLQ
RQISSIDFVKACYGK"
gene complement(<451234..>452847)
/locus_tag="AWJ20_3849"
/db_xref="GeneID:30035915"
mRNA complement(<451234..>452847)
/locus_tag="AWJ20_3849"
/product="hypothetical protein"
/transcript_id="XM_018880883.1"
/db_xref="GeneID:30035915"
CDS complement(451234..452847)
/locus_tag="AWJ20_3849"
/inference="similar to AA sequence:KEGG_Orthology:K13076"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733530.1"
/db_xref="GeneID:30035915"
/translation="MKDDLLSREDVEVLIAEGNLVVIYHDRALKLNNWITKHPGGDKA
VLHMVGRDASDEIDVYHSDKTLKSLPAFQIGRVQLPWRNFLPPIQGGTFRTRAELDSD
EGVSLSDPDQRIPRWEKTDMKKSTRWTKQEQRTHEIIENFDRKLVESDREKYPPVDLD
TQTNIISKFRDLHKELREEGFFQCNYWGYVREASRISTLFILARVFFTWRYQSYGTIW
LTLSAICLGLAWHQLTFIAHDAGHLGITHDYQIDNIVGVIIADYIGGLSIGWWKRNHN
VHHFVTNDPVHDPDIQHLPFFAVSSKFFGNIKSTYYDRVLDYDIFAKTLLKVQNYMYY
PILCFGRFNLYRLSWEYLILGLGPRKGKGAWLRYFELVGMAFFFYWFFYRVVTCSLHS
ASERWLYVMVSHIVTMPLHVQITLSHFGMSTSDLGIDESFAQRQIRTTMDVDCPPWLD
YIHGGLQFQAIHHLFPRMPRHNLRAAQPKVIKFCDDLGLHYTIYGFTKGNKVVISKLA
DVAKQARILADCNAFCQKEMLSELGIHKT"
gene complement(<453209..>455224)
/locus_tag="AWJ20_3850"
/db_xref="GeneID:30035917"
mRNA complement(<453209..>455224)
/locus_tag="AWJ20_3850"
/product="Peptide-N4-(N-acetyl-beta-
glucosaminyl)asparagineamidase A"
/transcript_id="XM_018880885.1"
/db_xref="GeneID:30035917"
CDS complement(453209..455224)
/locus_tag="AWJ20_3850"
/codon_start=1
/product="Peptide-N4-(N-acetyl-beta-
glucosaminyl)asparagineamidase A"
/protein_id="XP_018733531.1"
/db_xref="GeneID:30035917"
/translation="MSISAWFTGNTATGYIALSDDNDRNRINTRKRNLALAAAIAVGV
VIITVGMFHDYRWHDGIYRPLPGRGRHGGIHHGGSHHGGISNLWSKQLLLSFDDNSDI
GKPAHGSQKVEDVFQVYAPPEDIGELVLNKTLIDNYVFDNSYGKPFVVSYTAPDQEFT
HVRLTLNATTYGRQFDRLAQVYLGGSEIWRTSTAEPGHRNISFSYVKDVSKYASLFKT
SDIDFTFALNNIVDEVYTGPITVSLSAQYFNVPETRSTSVAELLKKDVTSPDQVIAIA
PPTGLSWSAPSQKISTKLPALPKDAARVVVDVFASGNADEEFWYSHLLDPYSDWFPNG
ANEFGKHGPTRLVEVHINGVLAGVAIPFPVIYTGGYSPPLWSKIVGTNTYDVPSYQID
ITPFLPYLWSESGSAISLRVVNGDKSESAKTLISEDWIVSANVLVWQVPGVQGSGVSD
IPKNKDSLTRIVSPRYDSRVSQIIAFSDSISTSARLEYIGTDGEKEDLTVGWQQKLAL
SNVQSVSNGGDKHEIVFSSSTANKLVVDDDFEAVNAQFKESHGLVLNLKYDSFEDSFK
ISYVRGVSTNDALGLTSAGQNGTSAFAFTGPRQVSGHGDLETNYTRQSYITDEKYHRH
VRAANGSVLFDHVDCSKPGLYSVDSVSPIESLIQLDGLDLSFFASNE"
gene <455956..>456753
/gene="URA3"
/locus_tag="AWJ20_3851"
/db_xref="GeneID:30035918"
mRNA <455956..>456753
/gene="URA3"
/locus_tag="AWJ20_3851"
/product="orotidine-5'-phosphate decarboxylase"
/transcript_id="XM_018880886.1"
/db_xref="GeneID:30035918"
CDS 455956..456753
/gene="URA3"
/locus_tag="AWJ20_3851"
/inference="similar to AA sequence:KEGG_Orthology:K01591"
/note="Orotidine-5'-phosphate (OMP) decarboxylase;
catalyzes the sixth enzymatic step in the de novo
biosynthesis of pyrimidines, converting OMP into uridine
monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a
toxic compound; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 18812321]; GO_function: GO:0016831 -
carboxy-lyase activity [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0016829 - lyase activity [Evidence IEA];
GO_function: GO:0004590 - orotidine-5'-phosphate
decarboxylase activity [Evidence IEA,IEA]; GO_function:
GO:0004590 - orotidine-5'-phosphate decarboxylase activity
[Evidence IDA] [PMID 2061334]; GO_function: GO:0004590 -
orotidine-5'-phosphate decarboxylase activity [Evidence
IMP] [PMID 4550660]; GO_function: GO:0004590 -
orotidine-5'-phosphate decarboxylase activity [Evidence
IMP] [PMID 5651325]; GO_process: GO:0044205 - 'de novo'
UMP biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic
process [Evidence IEA]; GO_process: GO:0006207 - 'de novo'
pyrimidine nucleobase biosynthetic process [Evidence IMP]
[PMID 4550660]; GO_process: GO:0006207 - 'de novo'
pyrimidine nucleobase biosynthetic process [Evidence IMP]
[PMID 5651325]; GO_process: GO:0006222 - UMP biosynthetic
process [Evidence IDA] [PMID 2061334]; GO_process:
GO:0006222 - UMP biosynthetic process [Evidence IMP] [PMID
4550660]; GO_process: GO:0006222 - UMP biosynthetic
process [Evidence IMP] [PMID 5651325]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0006221 - pyrimidine nucleotide biosynthetic process
[Evidence IEA]"
/codon_start=1
/product="orotidine-5'-phosphate decarboxylase"
/protein_id="XP_018733532.1"
/db_xref="GeneID:30035918"
/translation="MSNTTLSYEARAAKHPSAVASKLLKLMAEKKTNLCASLDVRTTK
EFLDLLEKVGPYIAVVKSHIDIIDDFTYEGTIVPLLALAKKHNFLIFEDRKFADIGST
VKAQYSGGVYKIAKWSDITNAHSVPGAGIVNGLEEAAKETTSEERGLLMLAELSTKGS
LATGEYTAKTVEIARQNSQFVFGFIAQSRMPEQGDEDWLILTPGVGLDDKGDGLGQQY
RTVDQVVSTGTDVIIVGRGLFGKGRDPVAESKRYRDAGWAAYEKRLA"
gene complement(<458453..>460072)
/gene="DBP3"
/locus_tag="AWJ20_3852"
/db_xref="GeneID:30035919"
mRNA complement(<458453..>460072)
/gene="DBP3"
/locus_tag="AWJ20_3852"
/product="RNA-dependent ATPase DBP3"
/transcript_id="XM_018880887.1"
/db_xref="GeneID:30035919"
CDS complement(458453..460072)
/gene="DBP3"
/locus_tag="AWJ20_3852"
/inference="similar to AA sequence:KEGG_Orthology:K14811"
/note="RNA-Dependent ATPase, member of DExD/H-box family;
involved in cleavage of site A3 within the ITS1 spacer
during rRNA processing; not essential for growth, but
deletion causes severe slow-growth phenotype;
GO_component: GO:0005730 - nucleolus [Evidence IEA];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
9032262]; GO_component: GO:0005634 - nucleus [Evidence
IEA]; GO_component: GO:0030687 - preribosome, large
subunit precursor [Evidence IDA] [PMID 22083961];
GO_function: GO:0005524 - ATP binding [Evidence IEA,IEA];
GO_function: GO:0004004 - ATP-dependent RNA helicase
activity [Evidence IDA] [PMID 23153376]; GO_function:
GO:0008026 - ATP-dependent helicase activity [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence
IEA]; GO_function: GO:0008186 - RNA-dependent ATPase
activity [Evidence IDA] [PMID 18975973]; GO_function:
GO:0008186 - RNA-dependent ATPase activity [Evidence IDA]
[PMID 23153376]; GO_function: GO:0004386 - helicase
activity [Evidence IEA,IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0003676
- nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0006200 - ATP catabolic process [Evidence
IEA]; GO_process: GO:0000464 - endonucleolytic cleavage in
ITS1 upstream of 5.8S rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 9032262]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA]"
/codon_start=1
/product="RNA-dependent ATPase DBP3"
/protein_id="XP_018733533.1"
/db_xref="GeneID:30035919"
/translation="MSTEKEIKSSKRSLEEAEKNESTKKLKVKSDKKDHKEEKNKKEK
KEKKDKKEKKEKKDKKDKKEKKEKKEKKDKEEKKESKSDDTVSSEQTAVPAAAAAAAA
PSSAEVTKFLADNSVKIEDDSNSNIQPCLSFDSLPVESKIISILKKFPKPSPIQAASW
PFLLAGRDVVGVAETGSGKTFAFSVPVVNHIIQEKKKGVRALVVSPTRELAMQIYDSL
VTLTNVTDISAVCVYGGVPKEEQRQGLKKASIVIATPGRLKDLIEEGSADLSKVEYLV
LDEADRMLEKGFEEDIKSIVSNTNSDGRQTIMFTATWPPEVRKLASTFMNNAVKVYIG
ERDELAANKRITQLVEVIDPFRKEQRLLQLLKEHQSGAKKDDKILVFALYKKEATRVE
RLLQNRGFALAAIHGDLGQAQRTKALEDFKTGRSKILLATDVAARGLDIPAVKVVINL
TFPLTAEDYVHRIGRTGRAGQTGLAITLFTEQEKHLSGALINVLRGANQPVPDELLKF
GSHTKKKEHSAYGAFFKDVDSTKKATKVKFD"
gene <461851..>463677
/gene="ERO1"
/locus_tag="AWJ20_3853"
/db_xref="GeneID:30035920"
mRNA <461851..>463677
/gene="ERO1"
/locus_tag="AWJ20_3853"
/product="Ero1p"
/transcript_id="XM_018880888.1"
/db_xref="GeneID:30035920"
CDS 461851..463677
/gene="ERO1"
/locus_tag="AWJ20_3853"
/note="Thiol oxidase required for oxidative protein
folding in the ER; essential for maintaining proper redox
balance in ER; feedback regulation of Ero1p occurs via
reduction and oxidation of Ero1p regulatory bonds; reduced
Pdi1p activates Ero1p by direct reduction of Ero1p
regulatory bonds; depletion of thiol substrates and
accumulation of oxidized Pdi1p results in inactivation of
Ero1p by both Pdi1p-mediated oxidation and autonomous
oxidation of Ero1p regulatory bonds; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IEA,IEA];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 9659914]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA]; GO_function: GO:0016671 - oxidoreductase
activity, acting on a sulfur group of donors, disulfide as
acceptor [Evidence IEA]; GO_function: GO:0003756 - protein
disulfide isomerase activity [Evidence IEA]; GO_function:
GO:0003756 - protein disulfide isomerase activity
[Evidence IMP] [PMID 10982384]; GO_function: GO:0016972 -
thiol oxidase activity [Evidence IDA] [PMID 16407158];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IGI] [PMID
11584268]; GO_process: GO:0006457 - protein folding
[Evidence IMP] [PMID 9659913]"
/codon_start=1
/product="Ero1p"
/protein_id="XP_018733534.1"
/db_xref="GeneID:30035920"
/translation="MSKIFYLTLLATVLASSSPFPSPPLEASVASQTVYDFLPIASDS
PVKDTLGGATYQQVEDTNFRLRPVLKELTKTDYFKYYKVDLSPELCPFNDDELGMCGN
RACAVDVIDDESEVPEFWRSQYLGKLAKDSVTTDRLWSPDDDAVASKLCRANGVSVGH
EYYNVLPSPSPPSKDDYLDSELEDTNYCYPEDESLSGPGTYVSLPDNPERFTGYGGPH
ANKVWRAVYQENCFGYFGDSNQDSGTSNAVSSGDGFSAGQLQPSLPAAGSLLSSVLMD
SNRYERQEVGGESGRQALNAEKQCVEQRLFYRILSGLQSSVSSHLCYEYLNQTTGEWV
PNLDCFMSRVGNHQERLENLYFNYALVSRAVSKLRNYIDDLHFNNQDASADQATRRQL
LRLIKSSSPVTYSRAQANDTNTPTSASSSQQKLLLFNETAIFSSPEGRALKSEFRKRV
RNVSALMSCVGCDRCRLWGKLQTAGYGTALKLLFELPENPADDPELCSTVMSTFRRSE
LVALINTLDRLSKSIEAVTYFRNELQSQAVEAEKLAERSEFQQAWDSELEGAWTALKF
VFRSYVELPKNLWTLFIHYSAIYWNRFIGRDALTAQYYHLDL"
gene complement(<464898..>465311)
/gene="RRT12"
/locus_tag="AWJ20_3854"
/db_xref="GeneID:30035921"
mRNA complement(<464898..>465311)
/gene="RRT12"
/locus_tag="AWJ20_3854"
/product="Rrt12p"
/transcript_id="XM_018880889.1"
/db_xref="GeneID:30035921"
CDS complement(464898..465311)
/gene="RRT12"
/locus_tag="AWJ20_3854"
/note="Probable subtilisin-family protease; role in
formation of the dityrosine layer of spore walls;
localizes to the spore wall and also the nuclear envelope
and ER region in mature spores; GO_component: GO:0005619 -
ascospore wall [Evidence IDA] [PMID 19779569];
GO_component: GO:0005635 - nuclear envelope [Evidence IDA]
[PMID 19779569]; GO_component: GO:0031160 - spore wall
[Evidence IEA]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0042802 -
identical protein binding [Evidence IEA]; GO_function:
GO:0008233 - peptidase activity [Evidence IEA];
GO_function: GO:0004252 - serine-type endopeptidase
activity [Evidence IBA,IEA]; GO_function: GO:0008236 -
serine-type peptidase activity [Evidence IEA];
GO_function: GO:0008236 - serine-type peptidase activity
[Evidence ISS] [PMID 10592175]; GO_process: GO:0030476 -
ascospore wall assembly [Evidence IMP] [PMID 19779569];
GO_process: GO:0043086 - negative regulation of catalytic
activity [Evidence IEA]; GO_process: GO:0006508 -
proteolysis [Evidence IBA,IEA,IEA]; GO_process: GO:0030435
- sporulation resulting in formation of a cellular spore
[Evidence IEA]"
/codon_start=1
/product="Rrt12p"
/protein_id="XP_018733535.1"
/db_xref="GeneID:30035921"
/translation="MSLGTPKNNILNRAVQQLVDMGVPVVVAAGNSDSNACRFSPSSA
QGAFVVGALDDRLDTAATFTNWGECVDAFASGVNVESISIFNNSPVKYSGTSVSSPIA
AGLIAYFMGMGDSGEVATQRVSKLISIILSRLGPN"
gene complement(<466442..>467893)
/gene="RRD1"
/locus_tag="AWJ20_3855"
/db_xref="GeneID:30035922"
mRNA complement(<466442..>467893)
/gene="RRD1"
/locus_tag="AWJ20_3855"
/product="peptidylprolyl isomerase RRD1"
/transcript_id="XM_018880890.1"
/db_xref="GeneID:30035922"
CDS complement(466442..467893)
/gene="RRD1"
/locus_tag="AWJ20_3855"
/inference="similar to AA sequence:KEGG_Orthology:K17605"
/note="Peptidyl-prolyl cis/trans-isomerase; activator of
the phosphotyrosyl phosphatase activity of PP2A; involved
in G1 phase progression, microtubule dynamics, bud
morphogenesis and DNA repair; required for rapid reduction
of Sgs1p levels in response to rapamycin; subunit of the
Tap42p-Sit4p-Rrd1p complex; protein increases in abundance
and relative distribution to the nucleus increases upon
DNA replication stress; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 15150670]; GO_component:
GO:0000790 - nuclear chromatin [Evidence IDA] [PMID
21129186]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 15150670]; GO_function: GO:0016853 - isomerase
activity [Evidence IEA]; GO_function: GO:0003755 -
peptidyl-prolyl cis-trans isomerase activity [Evidence
IEA,IEA]; GO_function: GO:0003755 - peptidyl-prolyl
cis-trans isomerase activity [Evidence IDA] [PMID
16380387]; GO_function: GO:0019211 - phosphatase activator
activity [Evidence IEA]; GO_function: GO:0008601 - protein
phosphatase type 2A regulator activity [Evidence IMP]
[PMID 12952889]; GO_process: GO:0006281 - DNA repair
[Evidence IMP] [PMID 15150670]; GO_process: GO:0006281 -
DNA repair [Evidence IMP] [PMID 9705277]; GO_process:
GO:0000082 - G1/S transition of mitotic cell cycle
[Evidence IPI] [PMID 11134337]; GO_process: GO:0030472 -
mitotic spindle organization in nucleus [Evidence IMP]
[PMID 11262194]; GO_process: GO:0043085 - positive
regulation of catalytic activity [Evidence IEA];
GO_process: GO:0006457 - protein folding [Evidence IEA];
GO_process: GO:0000413 - protein peptidyl-prolyl
isomerization [Evidence IEA,IEA]; GO_process: GO:0043618 -
regulation of transcription from RNA polymerase II
promoter in response to stress [Evidence IMP,IPI] [PMID
21129186]; GO_process: GO:0006970 - response to osmotic
stress [Evidence IMP] [PMID 10660069]"
/codon_start=1
/product="peptidylprolyl isomerase RRD1"
/protein_id="XP_018733536.1"
/db_xref="GeneID:30035922"
/translation="MTENIYNGSIVSGTIGTPGSGGSGSSEASVSSTLPSGTGKSQPL
LAPEDPFGTGETYSVEFPIHPSSYSTPGKKIHDQSDLIYFQQSIAYNRIQNLITSISQ
AVIQKFPADINSSSIHPVFKSIIEILQTLSKWCDEIEPVIGPRRFGNIAFRQWHTVLE
NRSGQLLTEKLAEPLAFSTIGDEMSDKVSAENSPLTELIPYFQGSFGSKQRLDFGTGH
ELSFLAFIGGLILLGVIDQPSGEDILLVFETYFEVIRKLVLRYTLEPAGSHGVWGLDD
HFHLPYILGSAQLVDPQSNGIPKPSSIISKDVPTPRSVLDKASVSKYARTNLYFKAID
FVYQVKRGPFYEHSPILYDITGVPTWNKIYRGMIKMYNAEVLGKFPVVQHFIFGSALY
PWKHINGNGLKSHSGEEEKAAPRKDASAVPSMVSSVMPLSATVSGVRPRASNAPRTSI
NPITGSSIGEPSTGSPTGGGNIPGPTKASWAKK"
gene <468646..>473577
/gene="NUP192"
/locus_tag="AWJ20_3856"
/db_xref="GeneID:30035923"
mRNA <468646..>473577
/gene="NUP192"
/locus_tag="AWJ20_3856"
/product="Nup192p"
/transcript_id="XM_018880891.1"
/db_xref="GeneID:30035923"
CDS 468646..473577
/gene="NUP192"
/locus_tag="AWJ20_3856"
/inference="similar to AA sequence:KEGG_Orthology:K14310"
/note="Essential subunit of the inner ring of the nuclear
pore complex (NPC); contributes to nucleocytoplasmic
transport; homologous to human NUP205; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0031965 - nuclear membrane [Evidence IEA];
GO_component: GO:0005643 - nuclear pore [Evidence
IEA,IEA,IEA]; GO_component: GO:0005643 - nuclear pore
[Evidence IDA] [PMID 10684247]; GO_component: GO:0044611 -
nuclear pore inner ring [Evidence IDA] [PMID 18046406];
GO_component: GO:0044615 - nuclear pore nuclear basket
[Evidence IDA] [PMID 10428845]; GO_component: GO:0005634 -
nucleus [Evidence IEA]; GO_function: GO:0017056 -
structural constituent of nuclear pore [Evidence IMP]
[PMID 10428845]; GO_process: GO:0051028 - mRNA transport
[Evidence IEA]; GO_process: GO:0006999 - nuclear pore
organization [Evidence IMP] [PMID 10428845]; GO_process:
GO:0006999 - nuclear pore organization [Evidence IMP]
[PMID 11121302]; GO_process: GO:0006913 -
nucleocytoplasmic transport [Evidence IC] [PMID 10684247];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Nup192p"
/protein_id="XP_018733537.1"
/db_xref="GeneID:30035923"
/translation="MLSEELNIDEVVAAEMVYNSSDDLNRLDMSPLQAGVALFHVRRQ
YILDLIRYFLVESGSDDRISDEFRKIVKSWSANGNDAALDSILEALKSVEEAYAELNE
KEKRGQFLGQMNNAEFVQLLKLRRDFLLREHDYLGQILCGLVQNRMVDAKGFAKLISK
VQTLSEYDTMFVHYIPSLLLYMSTFDPALTGTGYSVDFEEAYNGYKELSGDAQGWKLD
YWKGALSLAMLSGFSGCCRADTGDENGSGTGSNLIKYNISYELSILDPTKQAIDIGGI
EFLMILAANTSQTLSKKPHFYDYRPSVQNKVPSLKRLGDFSDEFLALLLQELERLVES
VVTNLADILKEMRLNEEDAFLSLTSVNNEFDHEEVDMPGADLERFFIFVSYLYSDRPD
ASINFWLDPESSLYGFTTWARQSHISFMAATLCDMLASLASGKECANAVYQLLKDDSD
SEENGEMGTKINAAAKGFSMGLAKLDNGNNNMLKRMNNTSSSYRLTWDVIFDALDYYA
KQLQPHSAAPSSSLSMGGLGAANGRQNALVLVTDMPELDDDIVLLLSAYFRLITVTIS
NDPEARLEFTTAVGGKYISTLFELLHSQTSLYGSILMTLAGFAKTDNEQIRYALWTAV
DNWLFNSTVYSPDGTVIAPSLPPRERLAQLFRSSSDIVGLITLLEALMSPLPQSARQG
FPFPQDLGSKYRYPGIWPYVDFIINEVFLSSASPTFTQDERLFIQLPCLLFIQYSLEL
LDPDVTRLSSTLGINPDIVVRSPSFLSYLYNHPSAPTMSHLFSSKIYSILISIASAGI
DEIGELGPDEPLVQAVVHSIRIMNDILDLQQIFLDVIVKEFKHGSSANGPAVDGVPTV
PPLQIVTHGLKAFEDVLLFNLPTVIHLALYIGSSNIELASRSLQLLQRLSLSSQFVST
VSTDARVKKNRLLSTLETADESNRIKEGFLEQLERPVDSYVEFNNSGSLPAIEIKLAL
LDFIICNLKTKSREPTTAHFLLGFKTTGDGKLDLDDGRCGIATGVSSFNSILSLLQYG
IENISGTDVEYGASKLSNACMEIILLLVQDPLSSQPILQLLRDTDFALSSLMAEPVVG
QDSLWNGQPYESTAAFSTIVSFFNHRSSLLEYVSLEIHESAELGSLSLVLRYVDSLVN
LDVTKKLSVNHQDSEHSAAMLSLLDVLEMPSRDQEPLQESSLEFFGSSVLNQIFSILG
NSIDPYANDLDTDNESAMSELNHLLKLKGLEFVANGKIQSLEDGVFKAAVEDIVSFMY
NSRTGGLIRDTQLKCLRSWSKLALVLVNDANVPSTKLITFILEIFQTLIPKLVDYSSS
DIAFAEVLASLLVSLQHIYQNEAKVGHGNSELNTAGYDRSHSLFRASLESIQTPLATP
ELRADLYIICYQYLKSTLDSPQAQQVIQVVRAAGDRLLETICGDALSGEGVTRLVALI
FLEVLFSLSSKTRSTFVQDGLVRYNLLLLLVKSIQRTDHEISNRDSLSPSELFFELKV
FNATLCFLLQVARTRSGASQLIQSGLFDILDGCQFLNIDLDVGIEISSPIDHRAGAVS
DLKISFYEILVPVFQILCAVLLSMGAENEPVITRVRRFLEGHQQLVVAIMKKDMLQSS
RPPSGDESSEVGLDDLVKLTVLLVSLTEFVPNL"
gene complement(<473749..>476502)
/gene="NSP1"
/locus_tag="AWJ20_3857"
/db_xref="GeneID:30035924"
mRNA complement(join(<473749..475815,476488..>476502))
/gene="NSP1"
/locus_tag="AWJ20_3857"
/product="FG-nucleoporin NSP1"
/transcript_id="XM_018880892.1"
/db_xref="GeneID:30035924"
CDS complement(join(473749..475815,476488..476502))
/gene="NSP1"
/locus_tag="AWJ20_3857"
/inference="similar to AA sequence:KEGG_Orthology:K14306"
/codon_start=1
/product="FG-nucleoporin NSP1"
/protein_id="XP_018733538.1"
/db_xref="GeneID:30035924"
/translation="MLRQTPFAFGSSTTPAGNPSGGNAFSFGNASNNTASGTTASTGT
GLFGTNTTGTGLFGSKPAATTTAPSGTGLFGATAGSTNSGTGSTGLFGGASTGTAAKP
LFGAATSTTPATSGTGLFGASTNNNASSTPASGTPSLFGSSTTGTSNASKPLFGSVAT
STTSSEASKPLFGAATATSQPAPSLFGGAASSTTTNTTSSGLTKPLFGTAASTGTSTE
PAKPLFGAATTTSATTEPAKPLFGAATTSTSSSQPAKPLFGAATTSTSSSQPAKPLFG
AAATGTSSEPAKPLFGAATTGTSSEPAKPLFGAAATGASTEPAKPLFGAAASTATTSA
EPAKPLFGAASTSTSTPLFGAKPATSEPAKPLFGAATTNTATSSKPAETAKPLFGAAT
TTSTSTTEPAKPLFGAPAATSSSTTNTTSSEPAKPLFGGATSTAKTTEPAKPLFGAAP
TTTSTTTATTSAPTTSGTKAASASSTTAPATKPEEIAPSLYLKNKSFEDIIAKWTSTL
AQNTADFQSQAISIAGWDRVLVENGERISEVFSEVVLAEQNQGKVDQMLQYISRQQDD
LETLLEGYEKQASHFLSDMAGPEGMQPVDQEREKAYRVAEDLNEKLESMDSNLASVIE
EINKVSSDLTRAKDDDALTQIVKILNSHLSSLRWIDSNTSLLQEKLDKIHTLESQTRD
HLNESQRFGAY"
gene <477310..>480333
/gene="GCV2"
/locus_tag="AWJ20_3858"
/db_xref="GeneID:30035925"
mRNA <477310..>480333
/gene="GCV2"
/locus_tag="AWJ20_3858"
/product="glycine decarboxylase subunit P"
/transcript_id="XM_018880893.1"
/db_xref="GeneID:30035925"
CDS 477310..480333
/gene="GCV2"
/locus_tag="AWJ20_3858"
/inference="similar to AA sequence:KEGG_Orthology:K00281"
/note="P subunit of the mitochondrial glycine
decarboxylase complex; glycine decarboxylase is required
for the catabolism of glycine to 5,10-methylene-THF;
expression is regulated by levels of 5,10-methylene-THF in
the cytoplasm; GO_component: GO:0005960 - glycine cleavage
complex [Evidence ISS] [PMID 8830251]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0004375
- glycine dehydrogenase (decarboxylating) activity
[Evidence IEA,IEA]; GO_function: GO:0004375 - glycine
dehydrogenase (decarboxylating) activity [Evidence IDA]
[PMID 320197]; GO_function: GO:0004375 - glycine
dehydrogenase (decarboxylating) activity [Evidence IDA]
[PMID 8830251]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA]; GO_function: GO:0030170 -
pyridoxal phosphate binding [Evidence IEA]; GO_process:
GO:0006546 - glycine catabolic process [Evidence IEA];
GO_process: GO:0019464 - glycine decarboxylation via
glycine cleavage system [Evidence IEP,IMP] [PMID 8830251];
GO_process: GO:0006544 - glycine metabolic process
[Evidence IEA]; GO_process: GO:0006730 - one-carbon
metabolic process [Evidence IGI] [PMID 10871621];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]"
/codon_start=1
/product="glycine decarboxylase subunit P"
/protein_id="XP_018733539.1"
/db_xref="GeneID:30035925"
/translation="MRFLIRSSQRLVAGRYAVNTTSRVFTRGALIGSRSYSSQIPSSS
PLEPLDTYLRRHNGSSSPKSIETLLDKVGYKSVDSFVESVVPDGILSRRPLQISPENG
LSESQLTSRLREIASENKLYRSFIGRGYYGTIVPPVIQRNILECPEWYTSYTPYQPEI
AQGRLESLMNFQTLVADLTGMSVANASLLDEATAGAEAMAMGFHALRGKRKVYYVDEN
VHSQTISVLKTRAKTLGIEVVIGLPKDEAEFKSSFGTLIQYPGANGNIESPEYYQQLS
DKVHGSGGVLAVATDLLALTVLKAPSSFGADIVFGNSQRFGVPFGYGGPHAAFFAVAD
SEKRKMPGRLIGRSKDRLGNPAFRLALQTREQHIRREKATSNICTAQALLANMAAMYA
VYHGPEGLKNIANRVYSLTTYLASQIESSSSHSLVNNKWFDTLTVKLGGVTADEFLAK
ARSEYQINLFKVDDSSVSVALDETVTAKDVSDLVSLFTGSTVGLTTLSAADVQYTSVP
EDLARTGKILQFAVFNTHHSETELLRYIHHLQSKDLSLANSMIPLGSCTMKLNATVQM
APVTWPEFGQIHPFAPIDQAKGYQTLVSELEADLADITGFDATSLQPNSGAQGEFAGL
KVIRAYLDSIGQGHRDICIIPVSAHGTNPASAAMAGFKVVSVKCTNNGELDLVDLEAK
AEKYKDKLAATMITYPSTYGVFEPGVKRAIEIVHSHGGQVYMDGANMNAQIGLTSPGE
IGADVCHLNLHKTFCIPHGGGGPGVGPICVKSHLQPFLPSHDTVAVTSASSDGRAVDS
VSAAPYGSASILPISWAYIKLMGASGLKKATELALLNANYMKARVGEHFPVAYTNAEG
RCAHEFIVDLRGFKSTSGIEAIDVAKRLQDYGFHAPTMSWPVPNTLMIEPTESESIAE
LDRFCDALISIRKEIADIEEGRVPRENNVLKNAPHSQQDLLTSEWDRPYTREQAAYPL
PYLREAKFWPSVTRLDDNYGDLNLFCSCDPVEAIE"
gene <482919..>483815
/gene="TRM7"
/locus_tag="AWJ20_3859"
/db_xref="GeneID:30035926"
mRNA <482919..>483815
/gene="TRM7"
/locus_tag="AWJ20_3859"
/product="Trm7p"
/transcript_id="XM_018880894.1"
/db_xref="GeneID:30035926"
CDS 482919..483815
/gene="TRM7"
/locus_tag="AWJ20_3859"
/inference="similar to AA sequence:KEGG_Orthology:K14864"
/note="2'-O-ribose methyltransferase; methylates the
2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at
positions C32 and N34 of the tRNA anticodon loop; crucial
biological role likely modification of tRNA-Phe; interacts
with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34
substrate tRNAs, respectively; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11927565]; GO_function:
GO:0008168 - methyltransferase activity [Evidence
IEA,IEA]; GO_function: GO:0008175 - tRNA methyltransferase
activity [Evidence IEA]; GO_function: GO:0008175 - tRNA
methyltransferase activity [Evidence IDA] [PMID 11927565];
GO_function: GO:0008175 - tRNA methyltransferase activity
[Evidence IMP,ISS] [PMID 9917067]; GO_function: GO:0016740
- transferase activity [Evidence IEA]; GO_process:
GO:0001510 - RNA methylation [Evidence IEA]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence IMP] [PMID
11927565]; GO_process: GO:0032259 - methylation [Evidence
IEA,IEA]; GO_process: GO:0030488 - tRNA methylation
[Evidence IDA] [PMID 11927565]; GO_process: GO:0008033 -
tRNA processing [Evidence IEA,IEA]"
/codon_start=1
/product="Trm7p"
/protein_id="XP_018733540.1"
/db_xref="GeneID:30035926"
/translation="MGKSSKDKRDLYYRKAKEEGWRARSAFKLLQLDSQFHLLKGLNR
VVDLCAAPGSWSQVLSRSLPEDSAKIVAVDLQPMAPIKGVTSIQADITHPKTLKRILD
IFGGEPADFVCSDGAPDVTGLHDLDEYIQAQLILSALQLATALLRPGGAFVAKIFRGR
DIDLMYFQLGLLFERVTCAKPRSSRGSSLEAFIVCQGYKPRPGWVPSIINEVFSTEEY
FKKMGIERPLIVDLDGEYEEEERVVAPFVACGDLSEYDSDATYTIERSTARVSLDPVQ
SPTAPPYKTAIELKRKGIIKRT"
gene complement(<484050..>485798)
/gene="KRS1"
/locus_tag="AWJ20_3860"
/db_xref="GeneID:30035928"
mRNA complement(<484050..>485798)
/gene="KRS1"
/locus_tag="AWJ20_3860"
/product="lysine--tRNA ligase KRS1"
/transcript_id="XM_018880896.1"
/db_xref="GeneID:30035928"
CDS complement(484050..485798)
/gene="KRS1"
/locus_tag="AWJ20_3860"
/inference="similar to AA sequence:KEGG_Orthology:K04567"
/note="Lysyl-tRNA synthetase; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0005737
- cytoplasm [Evidence IDA] [PMID 330225]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0004812 - aminoacyl-tRNA ligase activity [Evidence
IEA,IEA]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0004824 - lysine-tRNA
ligase activity [Evidence IEA,IEA]; GO_function:
GO:0004824 - lysine-tRNA ligase activity [Evidence IDA]
[PMID 3888626]; GO_function: GO:0004824 - lysine-tRNA
ligase activity [Evidence IMP] [PMID 7628447];
GO_function: GO:0003729 - mRNA binding [Evidence IDA]
[PMID 23222640]; GO_function: GO:0003676 - nucleic acid
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_process:
GO:0006430 - lysyl-tRNA aminoacylation [Evidence IEA];
GO_process: GO:0006430 - lysyl-tRNA aminoacylation
[Evidence IDA] [PMID 3888626]; GO_process: GO:0006430 -
lysyl-tRNA aminoacylation [Evidence IMP] [PMID 7628447];
GO_process: GO:0006418 - tRNA aminoacylation for protein
translation [Evidence IEA]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="lysine--tRNA ligase KRS1"
/protein_id="XP_018733541.1"
/db_xref="GeneID:30035928"
/translation="MSDSAVKEATEQLQKTYLDEETGEQVSKTELKKRQKAREVAKKR
AEKAAAAAEKAAASGEAKPKAKRDDDINPNQYFELRSTQIKALKETKAPNPYPHKFFV
TIKINEFVEQYKHLKRGEELKDVEVSLAGRIHVKRESGTQLKFYVLHGEGTQVQVVAQ
AVGEETLPEIHDLLRRGDIVGVKGYPYRTSPKKGGEGEVSVFVKEIVLLTPSLRMLPT
DHYGFKDLETRYRQRYLDLIMNNSTRDKFITRSKIISYIRKFFDSRDFVEVETPMMNQ
IAGGATAKPFITHHNDLNMDLFMRIAPELYLKELVVGGLERVYEIGRQFRNEGIDMTH
NPEFTTCEFYQAYADVYDLMDTTELLFSEMVKEITGSYKVEYQPQGPEGKTWEIDFSR
PWKRLDMIETLEEKLNVKFPPGDQLHTAETNEFLRGVLAKVGLECSPPLTNARMLDKL
VGEYLEETCINPTFIFGHPQMMSPLAKYHRSRPGLCERFEVFVGTKEIANAYTELNDP
FDQRDRFEEQARQKDQGDDEAQLIDETFCQALEYGLPPTGGWGCGIDRIAMFLTNSNS
IKEVLLFPAMKPEVNN"
gene <486426..>487697
/gene="TFG2"
/locus_tag="AWJ20_3861"
/db_xref="GeneID:30035929"
mRNA <486426..>487697
/gene="TFG2"
/locus_tag="AWJ20_3861"
/product="transcription factor IIF subunit TFG2"
/transcript_id="XM_018880897.1"
/db_xref="GeneID:30035929"
CDS 486426..487697
/gene="TFG2"
/locus_tag="AWJ20_3861"
/inference="similar to AA sequence:KEGG_Orthology:K03139"
/note="TFIIF (Transcription Factor II) middle subunit;
involved in both transcription initiation and elongation
of RNA polymerase II; homologous to human RAP30;
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005674 - transcription factor TFIIF
complex [Evidence IEA]; GO_component: GO:0005674 -
transcription factor TFIIF complex [Evidence IDA] [PMID
12242279]; GO_component: GO:0005674 - transcription factor
TFIIF complex [Evidence IDA] [PMID 7995524]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0000991 - core RNA polymerase II binding
transcription factor activity [Evidence IDA] [PMID
14749386]; GO_function: GO:0001139 - core RNA polymerase
II recruiting transcription factor activity [Evidence IC]
[PMID 7995524]; GO_function: GO:0004386 - helicase
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0000166
- nucleotide binding [Evidence IEA]; GO_process:
GO:0051123 - RNA polymerase II transcriptional
preinitiation complex assembly [Evidence IC] [PMID
7995524]; GO_process: GO:0001111 - promoter clearance from
RNA polymerase II promoter [Evidence IC] [PMID 7995524];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0006368 -
transcription elongation from RNA polymerase II promoter
[Evidence IDA] [PMID 18362165]; GO_process: GO:0006367 -
transcription initiation from RNA polymerase II promoter
[Evidence IEA]; GO_process: GO:0006367 - transcription
initiation from RNA polymerase II promoter [Evidence IDA]
[PMID 1331084]; GO_process: GO:0006367 - transcription
initiation from RNA polymerase II promoter [Evidence IDA]
[PMID 18362165]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0001174 -
transcriptional start site selection at RNA polymerase II
promoter [Evidence IMP] [PMID 15572698]; GO_process:
GO:0001174 - transcriptional start site selection at RNA
polymerase II promoter [Evidence IMP] [PMID 20033062]"
/codon_start=1
/product="transcription factor IIF subunit TFG2"
/protein_id="XP_018733542.1"
/db_xref="GeneID:30035929"
/translation="MSAVDVKEEPGSPGFEDDVSLSPSSPRPSGSLDDQDVPTRDGAE
EEEEIFEDSLDLDTSGASSKVWLVRLPKFLMERWKDVDKISDSELGKVRIRNQQGNEP
WKVKLVLNDTPETADIPHEYDISLVKQVVDNTFVFTEKDLPKFSKPQDKETTNPTTAA
ANRLAALNKAKNKYNNRKNYFKNSSPGVGSDGNNGNSSDSGISSPRFVPYVKTIPKKT
ALVGTACHECLVMPSMKDPNYAKVVSQRKALESESSAAKVTFLNEMPGVNAATFGQSL
RNKPGAFIRSTKKDIAKSSEGKATRIPKNELLDLLFKLFEEYDYWSLKGLRERTKQPE
TYLKEVLDTMAILIKKGPYAMKYTLKPEFKQIKGKEGSLSEYMERTGANGDADNEPNG
NRNENGNASGNDEPTIEDDEDDDDVEMETIL"
gene <490103..>491218
/locus_tag="AWJ20_3862"
/db_xref="GeneID:30035930"
mRNA <490103..>491218
/locus_tag="AWJ20_3862"
/product="AraC family transcriptional regulator"
/transcript_id="XM_018880898.1"
/db_xref="GeneID:30035930"
CDS 490103..491218
/locus_tag="AWJ20_3862"
/codon_start=1
/product="AraC family transcriptional regulator"
/protein_id="XP_018733543.1"
/db_xref="GeneID:30035930"
/translation="MSDGSSSLSSYASPVTPSIGSKSTYENSFQDIDTFRVKLLCFMD
SEIKKRQFLDQIRGRCSIDLKALVEWESPLTVSQKKVEVEKLLPDGIKDDDSVSPTGA
VKWVTPDFVVVPRSPKRIFCRPHCPSRKHKPLESVRLQFPKSIYSAMKDYGLAPCGRC
RPLDSHYLPAQSRLLQSVELMKEKGYKAKLIDLARKAGMSKHHYDRSFSAKFELPPGK
FQKMKLAQLSKDEEQIDKHSDEPCSAPGTSSTTSTGSDMEDAGDFADFESRFKDPLQI
PSGELTFFSDSEPSSITEETIGTEEDFSSCEMLNSMEWFTNYTKEIVREEVNIAVFYN
SNEVGCNLEQPMTFEPESDIFSQGFYDFSCDFILNYN"
gene <491935..>493533
/locus_tag="AWJ20_3863"
/db_xref="GeneID:30035931"
mRNA <491935..>493533
/locus_tag="AWJ20_3863"
/product="hypothetical protein"
/transcript_id="XM_018880899.1"
/db_xref="GeneID:30035931"
CDS 491935..493533
/locus_tag="AWJ20_3863"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733544.1"
/db_xref="GeneID:30035931"
/translation="MVEATKTVSGSVPDLGTGSDPGALNNVIDLVSQLSEDGLAQLQK
KIELLKSRSGGTAGSSSSPSSSTTNIKSSTSTTVAGTLPTDAAASGTPPTASSTPIPP
TPSITTLDGSEKWGSKVIITTYPGQQGVKSVPIKWGAKDPLERGPVIVSRTTGSLKRR
NAIGAYSGSYSIYHALAVAINDLPVDHKPDFTNAQPPVDIGPFPQWEGVDRIVSMDPF
GHIAPWVYSDLAAKENLDIRPTIAVTKAHMQLAEIRDEVKSGKLKVDGKIVLNEQGDL
AVTKVAVEPVWYLPGVAKRFGIDELTLRRALFEDTGGSYPELITRGDIKIFLPPIGGL
TAYIFGDPAKVSDPNSKLALRIHDECNGSDVFGSDICTCRPYLLFGISEAVKEAQNGG
SGVVIYFRKEGRALGEVTKYLVYNARKRGGDNAAEYFKRTENIAGVKDMRFQVLMPDI
LHFLGITKIDRMLSMSNMKHDAIVEQGIKIIERVPIPDELIPPDSRVEIDAKIFSGYF
TTGKIVTAEDLQQVKGRQWEDIDH"
gene <494277..>496028
/gene="KIN3"
/locus_tag="AWJ20_3864"
/db_xref="GeneID:30035932"
mRNA <494277..>496028
/gene="KIN3"
/locus_tag="AWJ20_3864"
/product="serine/threonine protein kinase KIN3"
/transcript_id="XM_018880900.1"
/db_xref="GeneID:30035932"
CDS 494277..496028
/gene="KIN3"
/locus_tag="AWJ20_3864"
/inference="similar to AA sequence:KEGG_Orthology:K08857"
/codon_start=1
/product="serine/threonine protein kinase KIN3"
/protein_id="XP_018733545.1"
/db_xref="GeneID:30035932"
/translation="MVRPDEFEPLEVIGRGSFGQIRKVRRITNGEILARKEISYKSMN
QKEKAQLIAEFRILKSLVHPNIVQYLHHEHLVDEHIVHLYMEYCGGGDLAGLISQCRS
SNTLVPENVIWGVFTQLIMALYRCHYNEDPPPVPEDLFGNDSDTPPPPNPSTVILHRD
IKPDNVFLDENYSVKLGDFGLAKMLDQENRFARTYVGTPYYMSPEVLLDEPSTPSSDV
WSLGCVIYELCALHPPFQAKTHMALSQKIREGAFDDIPSCYSSTLSRTITACLNTNPL
QRPTTATLLRLDIIKLCRKEREILTRERELKAIEQILIEREHQMEQQLEQQRFQMEQE
IEQEMYNRLEQRLAGVLEAEVEKRVEIALKSVMANNNNPNQVAVSPIDTSMLVAPSSP
IPGMSTGRNVRGPRSIRDAIISPLRDMSNSQYRTTAIDSPLSYYPKNASNSNINSSNS
SNSSSNNTVNVNIEGLGLSDGSGTSSTNSGSSVSSTSSTGTSSDPTANWITSKKDPQR
VAPHYQAMHSSPGIGAAAAKARLLNTKGSTPGMLGRVAVQLQREAQDTTQIWDENIHG
DNMPSPFLRRWERRTVS"
gene complement(<496266..>497540)
/gene="ACT1"
/locus_tag="AWJ20_3865"
/db_xref="GeneID:30035933"
mRNA complement(<496266..>497540)
/gene="ACT1"
/locus_tag="AWJ20_3865"
/product="actin"
/transcript_id="XM_018880901.1"
/db_xref="GeneID:30035933"
CDS complement(496266..497540)
/gene="ACT1"
/locus_tag="AWJ20_3865"
/inference="similar to AA sequence:KEGG_Orthology:K11400"
/note="Actin; structural protein involved in cell
polarization, endocytosis, and other cytoskeletal
functions; GO_component: GO:0031011 - Ino80 complex
[Evidence IPI] [PMID 10952318]; GO_component: GO:0031011 -
Ino80 complex [Evidence IPI] [PMID 24034245];
GO_component: GO:0035267 - NuA4 histone acetyltransferase
complex [Evidence IDA] [PMID 10911987]; GO_component:
GO:0035267 - NuA4 histone acetyltransferase complex
[Evidence IPI] [PMID 15485911]; GO_component: GO:0000812 -
Swr1 complex [Evidence IDA] [PMID 14645854]; GO_component:
GO:0000812 - Swr1 complex [Evidence IDA] [PMID 14690608];
GO_component: GO:0000812 - Swr1 complex [Evidence IDA]
[PMID 16299513]; GO_component: GO:0030479 - actin cortical
patch [Evidence IDA] [PMID 3967297]; GO_component:
GO:0030479 - actin cortical patch [Evidence IDA] [PMID
6365930]; GO_component: GO:0030479 - actin cortical patch
[Evidence IDA] [PMID 8163554]; GO_component: GO:0005884 -
actin filament [Evidence IDA] [PMID 6217414];
GO_component: GO:0032432 - actin filament bundle [Evidence
IDA] [PMID 3967297]; GO_component: GO:0032432 - actin
filament bundle [Evidence IDA] [PMID 6365930];
GO_component: GO:0032432 - actin filament bundle [Evidence
IDA] [PMID 8163554]; GO_component: GO:0000142 - cellular
bud neck contractile ring [Evidence IDA] [PMID 3967297];
GO_component: GO:0000142 - cellular bud neck contractile
ring [Evidence IDA] [PMID 8163554]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005856 - cytoskeleton [Evidence IEA,IEA]; GO_function:
GO:0005524 - ATP binding [Evidence IEA]; GO_function:
GO:0004402 - histone acetyltransferase activity [Evidence
IDA] [PMID 10911987]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_function: GO:0005200 -
structural constituent of cytoskeleton [Evidence IDA]
[PMID 6217414]; GO_process: GO:0006281 - DNA repair
[Evidence IDA] [PMID 16135807]; GO_process: GO:0000916 -
actomyosin contractile ring contraction [Evidence IDA,IMP]
[PMID 9732290]; GO_process: GO:0030476 - ascospore wall
assembly [Evidence IDA] [PMID 17118118]; GO_process:
GO:0007119 - budding cell isotropic bud growth [Evidence
TAS] [PMID 10652251]; GO_process: GO:0034599 - cellular
response to oxidative stress [Evidence IGI] [PMID
17287397]; GO_process: GO:0001300 - chronological cell
aging [Evidence IMP] [PMID 15024029]; GO_process:
GO:0006897 - endocytosis [Evidence IMP] [PMID 8590801];
GO_process: GO:0030010 - establishment of cell polarity
[Evidence IGI] [PMID 9864365]; GO_process: GO:0000132 -
establishment of mitotic spindle orientation [Evidence
TAS] [PMID 10652251]; GO_process: GO:0006887 - exocytosis
[Evidence TAS] [PMID 10652251]; GO_process: GO:0031505 -
fungal-type cell wall organization [Evidence TAS] [PMID
10652251]; GO_process: GO:0016573 - histone acetylation
[Evidence IDA] [PMID 10911987]; GO_process: GO:0000001 -
mitochondrion inheritance [Evidence TAS] [PMID 10652251];
GO_process: GO:0009306 - protein secretion [Evidence
IGI,IMP] [PMID 10793147]; GO_process: GO:0000011 - vacuole
inheritance [Evidence IGI,IMP] [PMID 8978821]; GO_process:
GO:0030050 - vesicle transport along actin filament
[Evidence TAS] [PMID 10652251]"
/codon_start=1
/product="actin"
/protein_id="XP_018733546.1"
/db_xref="GeneID:30035933"
/translation="MTRVKPPQADDVTSIVIENGSTWTRVGYSGDVTPKHYIPTVYAK
DADGKHYYGEEVDVPRPNTDIFSPMHDGCVQDWDAMSSFWKWIYSDKLFIEPNELPLV
TVEPSWNSLANKQKALEVAFEDLQVPVFSLVKAPIATAYESARPTSLIIDIGSAVASV
TPVIDGSLVPKTVFHSRFAGDFVNLHILTYLQTKNITITPSYLVRKKSVLDPGQPVST
PDLYEYPDIKPSFHAYQTSKVLHAFKETTSQVSNVPFTSASPFARIGRPFEFPDGFNM
IFGPERLTTAEPLLKPTQFPLPQVNLPENSVGSAPGNTIMHGLGDLLYLSLSKLDITP
EVLITLLNNIIICGGTSLISGLTDRIKEEILHMFPTYAPRFYIPEQESHRKNVVWTGA
SVLAGLGTFDQTWITKQEYDEFGAELAEKRFK"
gene <498378..>500636
/gene="PRT1"
/locus_tag="AWJ20_3866"
/db_xref="GeneID:30035934"
mRNA <498378..>500636
/gene="PRT1"
/locus_tag="AWJ20_3866"
/product="Prt1p"
/transcript_id="XM_018880902.1"
/db_xref="GeneID:30035934"
CDS 498378..500636
/gene="PRT1"
/locus_tag="AWJ20_3866"
/inference="similar to AA sequence:KEGG_Orthology:K03253"
/note="eIF3b subunit of the eukaryotic translation
initiation factor 3 (eIF3); subunit of the core complex of
eIF3; essential for translation; part of a subcomplex
(Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and
tRNA(i)Met to ribosomes; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0005737
- cytoplasm [Evidence IPI] [PMID 9722586]; GO_component:
GO:0010494 - cytoplasmic stress granule [Evidence IDA]
[PMID 19470581]; GO_component: GO:0016282 - eukaryotic 43S
preinitiation complex [Evidence IEA]; GO_component:
GO:0033290 - eukaryotic 48S preinitiation complex
[Evidence IEA]; GO_component: GO:0033290 - eukaryotic 48S
preinitiation complex [Evidence IDA] [PMID 17242201];
GO_component: GO:0005852 - eukaryotic translation
initiation factor 3 complex [Evidence IEA,IEA];
GO_component: GO:0005852 - eukaryotic translation
initiation factor 3 complex [Evidence IDA,IPI] [PMID
9660829]; GO_component: GO:0005852 - eukaryotic
translation initiation factor 3 complex [Evidence IDA,IPI]
[PMID 9671501]; GO_component: GO:0043614 - multi-eIF
complex [Evidence IDA] [PMID 11018020]; GO_component:
GO:0043614 - multi-eIF complex [Evidence IDA] [PMID
15838098]; GO_function: GO:0003723 - RNA binding [Evidence
IEA,IEA,IEA]; GO_function: GO:0003676 - nucleic acid
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0003743
- translation initiation factor activity [Evidence
IEA,IEA,IEA]; GO_function: GO:0003743 - translation
initiation factor activity [Evidence IDA,IPI,ISS] [PMID
7876188]; GO_function: GO:0003743 - translation initiation
factor activity [Evidence IDA] [PMID 9671501];
GO_function: GO:0031369 - translation initiation factor
binding [Evidence IEA]; GO_process: GO:0001731 - formation
of translation preinitiation complex [Evidence IEA];
GO_process: GO:0006446 - regulation of translational
initiation [Evidence IEA]; GO_process: GO:0006412 -
translation [Evidence IEA]; GO_process: GO:0006413 -
translational initiation [Evidence IEA,IEA,IEA];
GO_process: GO:0006413 - translational initiation
[Evidence IMP] [PMID 3029094]; GO_process: GO:0006413 -
translational initiation [Evidence IDA] [PMID 9671501]"
/codon_start=1
/product="Prt1p"
/protein_id="XP_018733547.1"
/db_xref="GeneID:30035934"
/translation="MPEATKNDHPHNEEEELDIDFSDLEAKYATNPDDGLETFVVVDG
APIVPAAKVEVLTKVLLKLFRSVGNIKAEDGFFMPMDEATGKSKGYVFIEYETPQQAQ
EAVKTYHQKKLDQRHTLLVNKLGDVERYGFAGNVASEYVEPTIEPFKPQEHLKSWLTD
SQGRDQMVLHRGDNVGVFYNRKSQTPEQVVDRQHWTEKYVRWSPKGTYLVSIHRQGVA
LWGGDSWNRLGRFAHNDVRLIDFSPNEKYLISLSMEPISIPPPDSPARAACPFKEQDE
GNHVVVWDVKTMLPLRSFPLLPGEGPSAASKNGPPAPEKKIVWPMFKWSSNSKYFARV
IPDEALAIYEAPSMGLLDKKSIKIPGIVDFEFAPAPVKLEGHKATAATEDGGVAAGEQ
ILCYWTPEMNNQTARVSIMDVGTRKVIRSRNLFNVSGCRLHWQDQGKYLCVKVDRHTK
TKKSTFTNLEFFRLCDKDIPVEVLELKETVINFQWEPKSDRFVLISFLDQGPLATAPV
ANRNTVAFYAFERSKGSQGTWKPIKVQDKKATNSIYWSPKGRFVIAGTVRPSSACEFE
FWDFDHEGDVKAAGAEDLPANLVNIGSCEHYGMTDLEWDPSGRFVVTSSSFWRHAIEN
GYKMWDFRGSLVREEAIEKFKSFLWRPRPASILPKEKRKQVNKNLKSYSRRFDEEDAM
EESEASRQLILKRREALAEWREWRAEMEAHLHELGLTPESDLIAAADGDDDEVIEEIR
EEILEEKEEEIE"
gene complement(<504870..>505979)
/gene="IMD4"
/locus_tag="AWJ20_3867"
/db_xref="GeneID:30035935"
mRNA complement(<504870..>505979)
/gene="IMD4"
/locus_tag="AWJ20_3867"
/product="IMP dehydrogenase IMD4"
/transcript_id="XM_018880903.1"
/db_xref="GeneID:30035935"
CDS complement(504870..505979)
/gene="IMD4"
/locus_tag="AWJ20_3867"
/inference="similar to AA sequence:KEGG_Orthology:K00088"
/note="Inosine monophosphate dehydrogenase; catalyzes the
rate-limiting step in the de novo synthesis of GTP; member
of a four-gene family in S. cerevisiae, constitutively
expressed; IMD4 has a paralog, IMD3, that arose from the
whole genome duplication; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0005737
- cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 23222640];
GO_function: GO:0003938 - IMP dehydrogenase activity
[Evidence IEA,IEA,IEA]; GO_function: GO:0003938 - IMP
dehydrogenase activity [Evidence ISS] [PMID 11003640];
GO_function: GO:0030554 - adenyl nucleotide binding
[Evidence IEA]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0003729 - mRNA
binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA,IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_process: GO:0006177 - GMP
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]; GO_process: GO:0006164 - purine nucleotide
biosynthetic process [Evidence IEA,IEA]"
/codon_start=1
/product="IMP dehydrogenase IMD4"
/protein_id="XP_018733548.1"
/db_xref="GeneID:30035935"
/translation="MPGKLVGIVTSRDIQFHDNDSTPVSDIMTKRSELVTASHGIDLA
SANDILRKSKKGKLPIVDSDGNIIALLSRTDLKKNHNYPLASKSADSKQLICGAAIGT
RPDDRERLTKLAAAGLDLVILDSSQGNSVFQIEMIQWIKENYPHIEVVAGNVVTREQA
ASLIDAGADGLRIGMGSGSICITQEVMAVGRPQATAVYRVTEFANKFGVPCIADGGVQ
NIGHITKALALGASCVMMGGLLAGTTESPGQYFYREGQRLKAYRGMGSIEAMEKTGSN
DNAATSRYFSESDAVLVAQGVSGSVVDRGSITKFIPYLMSGLQHGLQDIGVRSVGELR
EKVDAGLVRFEHRTPSAQLEGGVHSLHSYEKRLHN"
gene complement(<506268..>506783)
/gene="IMD4"
/locus_tag="AWJ20_3868"
/db_xref="GeneID:30035936"
mRNA complement(<506268..>506783)
/gene="IMD4"
/locus_tag="AWJ20_3868"
/product="IMP dehydrogenase IMD4"
/transcript_id="XM_018880904.1"
/db_xref="GeneID:30035936"
CDS complement(506268..506783)
/gene="IMD4"
/locus_tag="AWJ20_3868"
/inference="similar to AA sequence:KEGG_Orthology:K00088"
/note="Inosine monophosphate dehydrogenase; catalyzes the
rate-limiting step in the de novo synthesis of GTP; member
of a four-gene family in S. cerevisiae, constitutively
expressed; IMD4 has a paralog, IMD3, that arose from the
whole genome duplication; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0005737
- cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 23222640];
GO_function: GO:0003938 - IMP dehydrogenase activity
[Evidence IEA,IEA,IEA]; GO_function: GO:0003938 - IMP
dehydrogenase activity [Evidence ISS] [PMID 11003640];
GO_function: GO:0030554 - adenyl nucleotide binding
[Evidence IEA]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0003729 - mRNA
binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA,IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_process: GO:0006177 - GMP
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]; GO_process: GO:0006164 - purine nucleotide
biosynthetic process [Evidence IEA,IEA]"
/codon_start=1
/product="IMP dehydrogenase IMD4"
/protein_id="XP_018733549.1"
/db_xref="GeneID:30035936"
/translation="MSPAVTKLSATQALAHLKEYEELDGLDVRTLMDPNIRGGLTYND
FLLLPGKIDFPSSIVNLESKISRNISLKLPLVSSPMDTVTESEMAIHMALLGGIGIIH
HNCTPDEQAAMVRKVKKYENGFISDPVVVAPTKTVGEVRALKESLGFAGFPVTGKCIF
QLVDLSGSDLI"
gene complement(<507742..>508815)
/gene="PEP4"
/locus_tag="AWJ20_3869"
/db_xref="GeneID:30035937"
mRNA complement(<507742..>508815)
/gene="PEP4"
/locus_tag="AWJ20_3869"
/product="proteinase A"
/transcript_id="XM_018880905.1"
/db_xref="GeneID:30035937"
CDS complement(507742..508815)
/gene="PEP4"
/locus_tag="AWJ20_3869"
/inference="similar to AA sequence:KEGG_Orthology:K01381"
/codon_start=1
/product="proteinase A"
/protein_id="XP_018733550.1"
/db_xref="GeneID:30035937"
/translation="MSRGSSGGEQKPLINENNVDIDLEIAAAKGGVDVPLTNYVNAQY
FTEISLGNPPQQFKVVLDTGSSNLWVPSSECGSIACYLHEKYDSSASSSYKSNGSDFE
IHYGSGSVSGFVSQDTLKIGSLTIPNQDFAEITSEPGLAFAFGKFDGILGLAYDTISV
NHIVPPIYNAIKDKLLDENKFSFHLGDTAKSAAGGTATFGGIDEDLFEGKLTYLPLRR
LAYWEVPFDSIALGNEVADLEDTGAAIDTGTSLLALPSGLAEILNAQIGAKKSWSGQY
TIDCAARDTLPDLTLNFAGYNFTLTAYDYTLEISGSCASAFTPLDIPAPIGPLAIIGD
SFLRRYYSVYDIDNNAVGLAKAK"
gene complement(<512551..>514062)
/locus_tag="AWJ20_3870"
/db_xref="GeneID:30035939"
mRNA complement(<512551..>514062)
/locus_tag="AWJ20_3870"
/product="ser/thr/tyr protein kinase RAD53"
/transcript_id="XM_018880907.1"
/db_xref="GeneID:30035939"
CDS complement(512551..514062)
/locus_tag="AWJ20_3870"
/codon_start=1
/product="ser/thr/tyr protein kinase RAD53"
/protein_id="XP_018733551.1"
/db_xref="GeneID:30035939"
/translation="MNHNIGTGRFLLKPLQDINLSKNGIDFIGSLLTVDAQHRPNATV
CLKLPWMMETEESFNSGESVLEELPITPARQAPAPPRPLPPIPPSAAQLAADSPVRST
VRPAAVTPGKVSGLPAPESPAVDPGPADLQSSYNEELPISSLQIRSSVEPEIPDNTDS
NDKSEQEKALKDYESLEAEYNSLPALAECDSDSDSQEIKEHVEAQLSQRSNKYGDSSM
QVESSFDSGIHLRNGLASSPGHKVLPFQQPGVRDFDSIGNADQPPPGAYMRLDTLPNS
IPFRDIYVTRPVFTLGRVPDNDVQVEERRVSKRHCEIIRVPHSGSGVKQEIWLVDRSS
NGCFLNGELIGAGKKALIESGDTICLFIDMDRDNKRKSIFHTHKHICSSDSLTYYAGH
ITLGFSANILDADDEGYLKRCQLIQEGGQPVTAAEPAVLATPSEDSDSSPIRKQLHSY
TDAPNNNNATDTRPNLSRLPSKKRAFGVSISCFAGATRVWGGAPAAGGSTTSH"
gene <517514..>519562
/locus_tag="AWJ20_3871"
/db_xref="GeneID:30035940"
mRNA join(<517514..517627,518288..>519562)
/locus_tag="AWJ20_3871"
/product="hypothetical protein"
/transcript_id="XM_018880908.1"
/db_xref="GeneID:30035940"
CDS join(517514..517627,518288..519562)
/locus_tag="AWJ20_3871"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733552.1"
/db_xref="GeneID:30035940"
/translation="MTTLDYEEPLTSTVRPNTAAFLTVRFIKSFPYRTVKNHRRLTAQ
TRGTKVTAEVLDSRTETVDNGSEKTNSVGQVTSDGSETSNKATKTGSGTTTGNGGERR
NRGQLTSTQTRRGVTKVSTEVLKSRTEAVDNGSEKTDSVRQVTSNGIETSNKATKTSS
GTTTGNGGERRNRGQLASTQTRRGVTKVSTEVLKSGTETVDNGSEKTNSVGQVTSDGS
ETSNKATKTGSGTTTGNGGERRNRGQLTSTQTRRGVTKVSTEVLKSRTETVDNGLKET
DSVGQVTSDGSEASNKATKTGSGTTTGNGGKRGNGGERRNRGQLTTAQTRSTKVTAEV
LKSRTETVDNGLEKTDSVGQVTSDGSETSNETTKTGSGTTTGNGGNGGERRNRGQLTT
AQTRSTKVTTEVLKSRTETVNNRLKETDSVGQVTSDGIKTSNETTKTSGSTTAGDLGH
RSSVNSCKRKGN"
gene complement(<518294..>519583)
/locus_tag="AWJ20_3872"
/db_xref="GeneID:30035941"
mRNA complement(<518294..>519583)
/locus_tag="AWJ20_3872"
/product="putative spidroin-1-like"
/transcript_id="XM_018880909.1"
/db_xref="GeneID:30035941"
CDS complement(518294..519583)
/locus_tag="AWJ20_3872"
/codon_start=1
/product="putative spidroin-1-like"
/protein_id="XP_018733553.1"
/db_xref="GeneID:30035941"
/translation="MRSTSYSLVALSLAAVNAAPMPQVPGGGAAAGLGGLVAGLDPVT
GDLSNTVGLLEPVVDSLGPALEHLGGDLGAPGLSRRQLPSVPSLPSVPSVPGGGAAAG
LGGLVAGLAPVTGDLSNTVGLLEPVVDSLGPALEHLGGDLGAPGLSRRQLPSVPSLPS
VPSLPSVPGGGAAAGLGGLVAGLAPVTGDLSNTVGLLEPVVDSLGPALEDLGADLGDA
SSGLSARQLPSVPSLPSVPGGGAAAGLGGLVAGLAPVTGDLSNTVGLLAPVVDSLGPA
LEDLGADLGDASSGLSARQLPSVPSLPSVPGGGAAAGLGGLVAGLDPVTGDLSNTVGL
LAPVVDSLGPALEDLGADLGDASSGLSARQLPSVPSLPSVPGGGAAAGLGGLVAGLAP
VTGDLSNTVGLLAPVVDSLGPAVEHLGGDLGAPGLSR"
gene complement(<521620..>522333)
/locus_tag="AWJ20_3873"
/db_xref="GeneID:30035942"
mRNA complement(<521620..>522333)
/locus_tag="AWJ20_3873"
/product="hypothetical protein"
/transcript_id="XM_018880910.1"
/db_xref="GeneID:30035942"
CDS complement(521620..522333)
/locus_tag="AWJ20_3873"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733554.1"
/db_xref="GeneID:30035942"
/translation="MRSTTYSLATALTLSVVLAAPAPQELPSVPGLPSLPGLPSLPGA
SGLPSVPGVSGLPSVPGVSGLPSVPGVSGLPSLPGVSGLPSLPGVSGLPSLPGASGVE
GLVGGLLDTVVGLLEPIIATLDPAAAAQAEAALANAGLKVRRQLPTGGAAALDGAGNE
VLDAVASIVEPLVAVLDPSAETQKGIFGIEKRDFHHGEHHGNHHGDHSHPHPPTPSPN
SEVDNLVDGLDSTIDSLNL"
gene <521975..>522265
/locus_tag="AWJ20_3874"
/db_xref="GeneID:30035943"
mRNA <521975..>522265
/locus_tag="AWJ20_3874"
/product="hypothetical protein"
/transcript_id="XM_018880911.1"
/db_xref="GeneID:30035943"
CDS 521975..522265
/locus_tag="AWJ20_3874"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733555.1"
/db_xref="GeneID:30035943"
/translation="MMGSRRPTTVSRRPPTRPSTPLAPGREGRPETPGREGRPETPGR
EGRPETPGTEGRPETPGTEGRPETPGTEGRPEAPGREGRPGREGRPGTEGSS"
gene <525204..>526226
/gene="MPM1"
/locus_tag="AWJ20_3875"
/db_xref="GeneID:30035944"
mRNA <525204..>526226
/gene="MPM1"
/locus_tag="AWJ20_3875"
/product="Mpm1p"
/transcript_id="XM_018880912.1"
/db_xref="GeneID:30035944"
CDS 525204..526226
/gene="MPM1"
/locus_tag="AWJ20_3875"
/note="Mitochondrial intermembrane space hypothetical
protein; GO_component: GO:0019898 - extrinsic component of
membrane [Evidence IDA] [PMID 11791739]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005758 - mitochondrial intermembrane space [Evidence
IDA] [PMID 22984289]; GO_component: GO:0005758 -
mitochondrial intermembrane space [Evidence IDA] [PMID
22993211]; GO_component: GO:0031966 - mitochondrial
membrane [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 11791739];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 9874242]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0008150 - biological_process [Evidence ND]"
/codon_start=1
/product="Mpm1p"
/protein_id="XP_018733556.1"
/db_xref="GeneID:30035944"
/translation="MTKDDDNRPGVNSEKDDTAKAWSELSGTLDEVWDLTDRTFGRLT
NLASVYGDKVQGALDDNWRNWLDADMIWRSSWKNDGSEGGNGSDDGENNESWHRRFHR
VRDCRKDSKMWGYPVPSQRQYDKCIENNGTSVWSRDGVWRCLFPESNANPMAIANAKD
VAASASASSGSSLENHQWFTDYSVYLDWRRAMRKAEAAKRESEWNRIKERKEKLFSFP
SDSDIESSARSTSISSGNSSGNNGSTAVSATSSAAKQITESEAEDQGKNVISKSIVVE
SVWRDGKLQTRQVVKKWYDDNTVSVKETTNSNANSNSNPALTSGTSEDGLNHHSNDSK
GGWFWK"
gene <527614..>528468
/gene="GLC8"
/locus_tag="AWJ20_3876"
/db_xref="GeneID:30035945"
mRNA <527614..>528468
/gene="GLC8"
/locus_tag="AWJ20_3876"
/product="Glc8p"
/transcript_id="XM_018880913.1"
/db_xref="GeneID:30035945"
CDS 527614..528468
/gene="GLC8"
/locus_tag="AWJ20_3876"
/inference="similar to AA sequence:KEGG_Orthology:K16833"
/note="Regulatory subunit of protein phosphatase 1
(Glc7p); involved in glycogen metabolism and chromosome
segregation; proposed to regulate Glc7p activity via
conformational alteration; ortholog of the mammalian
protein phosphatase inhibitor 2; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
11914276]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0008047 -
enzyme activator activity [Evidence IDA] [PMID 12127071];
GO_function: GO:0004864 - protein phosphatase inhibitor
activity [Evidence IEA]; GO_process: GO:0005975 -
carbohydrate metabolic process [Evidence IEA]; GO_process:
GO:0007059 - chromosome segregation [Evidence IMP] [PMID
7565759]; GO_process: GO:0005978 - glycogen biosynthetic
process [Evidence TAS] [PMID 9584086]; GO_process:
GO:0005977 - glycogen metabolic process [Evidence IEA];
GO_process: GO:0043086 - negative regulation of catalytic
activity [Evidence IEA]; GO_process: GO:0043666 -
regulation of phosphoprotein phosphatase activity
[Evidence IEA]; GO_process: GO:0009966 - regulation of
signal transduction [Evidence IEA]"
/codon_start=1
/product="Glc8p"
/protein_id="XP_018733557.1"
/db_xref="GeneID:30035945"
/translation="MSSPSPTGSPRSPHFTTERRRSSASSNVKPKSILRNAPPPGVYN
SNPGSPSVDSPAAFDRQAVLENTRANAKLNNIGAEIKRRNTLESGDQLTGDLDSLHNN
DHNNNSSNSTTSSNSSTAGVDQDSERAKQEHLKWDEANLYLTEQEKAATMKITEPKTP
YQGAVGDSEYYLPDEEDDVVKTRDNRILSVDELDGFSLGESVAPPAQVATQNDRILYR
ENDSNDAEDEDDEDEDDEDEEEEEEELTPEERHRRFEELRKSHYHMKAAALHTSVPAE
DDDEDDEN"
gene <531013..>532137
/gene="TUB2"
/locus_tag="AWJ20_3877"
/db_xref="GeneID:30035946"
mRNA <531013..>532137
/gene="TUB2"
/locus_tag="AWJ20_3877"
/product="beta-tubulin"
/transcript_id="XM_018880914.1"
/db_xref="GeneID:30035946"
CDS 531013..532137
/gene="TUB2"
/locus_tag="AWJ20_3877"
/inference="similar to AA sequence:KEGG_Orthology:K07375"
/note="Beta-tubulin; associates with alpha-tubulin (Tub1p
and Tub3p) to form tubulin dimer, which polymerizes to
form microtubules; mutation in human ortholog is
associated with congenital fibrosis of the extraocular
muscles (CFEOM) with polymicrogyria; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005881 - cytoplasmic microtubule [Evidence IC] [PMID
9488492]; GO_component: GO:0005856 - cytoskeleton
[Evidence IEA,IEA]; GO_component: GO:0005828 - kinetochore
microtubule [Evidence IC] [PMID 9488492]; GO_component:
GO:0005874 - microtubule [Evidence IEA,IEA]; GO_component:
GO:0005880 - nuclear microtubule [Evidence IC] [PMID
9488492]; GO_component: GO:0043234 - protein complex
[Evidence IEA]; GO_component: GO:0005816 - spindle pole
body [Evidence IDA] [PMID 9585415]; GO_component:
GO:0045298 - tubulin complex [Evidence IDA,IPI] [PMID
9488492]; GO_function: GO:0005525 - GTP binding [Evidence
IEA,IEA]; GO_function: GO:0003924 - GTPase activity
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0000166
- nucleotide binding [Evidence IEA]; GO_function:
GO:0005200 - structural constituent of cytoskeleton
[Evidence IEA]; GO_process: GO:0006184 - GTP catabolic
process [Evidence IEA]; GO_process: GO:0045143 -
homologous chromosome segregation [Evidence IC] [PMID
9488492]; GO_process: GO:0007017 - microtubule-based
process [Evidence IEA]; GO_process: GO:0000070 - mitotic
sister chromatid segregation [Evidence IC] [PMID 9488492];
GO_process: GO:0030473 - nuclear migration along
microtubule [Evidence IC] [PMID 9488492]; GO_process:
GO:0051258 - protein polymerization [Evidence IEA];
GO_process: GO:0046677 - response to antibiotic [Evidence
IEA]"
/codon_start=1
/product="beta-tubulin"
/protein_id="XP_018733558.1"
/db_xref="GeneID:30035946"
/translation="MDAVRSGPFGNLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNV
LDIVRREAEGCDSLQGFQIAHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVVPSPK
VSDTVVEPYNATLSINELVNYADETFCIDNEALYNICQQTLKMPQPTHGDLNQLISAV
MSGVTTCLRYPGQLNSDLRKLAVNLVPFPRLHFFTVGFAPLSAIGSKSFRSLTVPELT
QQMFDAKNMMAAADPRLGRYLTVAAFFRGKVSMKEVEDQMRSVQERNSAYFVEWIPNN
VQSAICSIPPKGLKMSATFVGNSTSIQELFKRVGDQFSAMFRRKAFLHWYINEGMDEM
EFTEAESNMNDLVSEYQQYQQAAAYEEELDYEDEAPIDEM"
gene <540215..>541075
/gene="CCC1"
/locus_tag="AWJ20_3878"
/db_xref="GeneID:30035947"
mRNA <540215..>541075
/gene="CCC1"
/locus_tag="AWJ20_3878"
/product="Ccc1p"
/transcript_id="XM_018880915.1"
/db_xref="GeneID:30035947"
CDS 540215..541075
/gene="CCC1"
/locus_tag="AWJ20_3878"
/note="Putative vacuolar Fe2+/Mn2+ transporter; suppresses
respiratory deficit of yfh1 mutants, which lack the
ortholog of mammalian frataxin, by preventing
mitochondrial iron accumulation; relative distribution to
the vacuole decreases upon DNA replication stress;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0005794 - Golgi apparatus [Evidence IDA]
[PMID 8866476]; GO_component: GO:0000139 - Golgi membrane
[Evidence IEA]; GO_component: GO:0000324 - fungal-type
vacuole [Evidence IDA] [PMID 23708375]; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 11390404]; GO_component: GO:0000329 - fungal-type
vacuole membrane [Evidence IDA] [PMID 22842922];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005774 - vacuolar membrane [Evidence
IEA]; GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0015093 - ferrous iron transmembrane
transporter activity [Evidence IMP] [PMID 11390404];
GO_function: GO:0005384 - manganese ion transmembrane
transporter activity [Evidence IMP] [PMID 11390404];
GO_process: GO:0006874 - cellular calcium ion homeostasis
[Evidence IGI,IMP] [PMID 7941738]; GO_process: GO:0006879
- cellular iron ion homeostasis [Evidence IGI,IMP] [PMID
10713071]; GO_process: GO:0030026 - cellular manganese ion
homeostasis [Evidence IGI,IMP] [PMID 8866476]; GO_process:
GO:0015684 - ferrous iron transport [Evidence IMP] [PMID
11390404]; GO_process: GO:0006828 - manganese ion
transport [Evidence IMP] [PMID 11390404]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Ccc1p"
/protein_id="XP_018733559.1"
/db_xref="GeneID:30035947"
/translation="MLVLLKNAIFGSNQDDRRSLLPTARSYDSVNTIGCDNCQDDDTC
SRSSSATASSFMESGIANPRVMSDIIIGLSDGLTVPFALTAGLSSLGDSKLVITGGLA
ELVSGAISMGLGGYLAARSESEYYEHQVAKEKATYAESPCLATQSIQEVLAEYKLSPE
TIKSFVADLEKSQEDTVNFIIRFARGLEEPASGREFTSAMTIGLAYFMGGFVPLIPYF
FANDVVTGLYISAIVMAITLFLFGSIKTVISLGKECGRSQILSNGVKMTLTGGVAAAA
AWGLVRAIDN"
gene complement(<541768..>546084)
/locus_tag="AWJ20_3879"
/db_xref="GeneID:30035948"
mRNA complement(<541768..>546084)
/locus_tag="AWJ20_3879"
/product="hypothetical protein"
/transcript_id="XM_018880916.1"
/db_xref="GeneID:30035948"
CDS complement(541768..546084)
/locus_tag="AWJ20_3879"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733560.1"
/db_xref="GeneID:30035948"
/translation="MSFFYEGDQLHYRKDKVSEENVSSWTVVDLPYRAAGIVPDLPSF
TAFLTQSFTLIPLLEVDLEIDGIQLLSLRKVVGPGTEMAIPKHLNTKSPNGFLKLQSL
DVESFQVKIKYMNVTQMSNDSLFGKSGNLFSFGKKLFSSLVTSSSNPSDITEVVCFLR
KVSGTIDVSVPASFAAKMKATVMKAPAKKAYISMLAYNKEEKELSELKPPLSDYIFPK
EFNDAKIYIGFPTKQSTSMRSHICMNQLIPTMERTAVDMSNQHVKDWNQQMLYISGVL
ARAIYENEMSRIAMTYSTSQVTDMLESACYIINRFEFERSTPDPNVGDLIAAGFWRSS
AKLSLPSEKGIKLSNEIRVADVNTSFIQQVPVLPYKVVSECPKFIERATYLGYLNKLS
VQDVSEDIQGHTLSIQNFKELILWLLGELKHKNITFERLKSVLDIAVVVLPGEGAGET
LFLRGVNSYQNKFVVEEKMPLPPTCAPFKFIKDIPWTTLHELGWEELELVDWLEYIVE
QKSSLPLEQNISISPDFAILVLQKISTNWNSFTTPTKRRVISLLHNETCIPTQLGMKL
PSESYLMDIPLFPNLPVKSPHLTADYKFLFTLGLRESVDMAFVLKLLHDPENSAVKWS
TSDMVKYLTDNRKSLKHSDWQLLKEKAFFEADDDNSKVLYKAGELYAPDPTLKKLGFR
TLKWNFWSNSGPEATLLFDIGLLRYPDEAALFKSAVSSESKLKIAIDYFVTNSAKNKY
SAQRAALIGEKIIPCNLNGQVVYRAPKDCFIDSQVGEFGLPVVRDQLLVTEGWKFGIR
KNPSMSELVNALRSKVPPSIAAAESMFTYLSTLVSFISPDHIKQLQSVAFIPIAVGQK
IKYQYPLKTFLEPLEKDAHEDLFFKEFFDFVGMPASATPFLLKVGVRYNPSTLEMAEA
AVDDPMSMYGLASSSDKYESFLYRLAEAWPELSKNYSLVSKMRTSRFLLALSYKGESC
EDGDDDGYSYRQKASYSLASASEIVIVNDVISFNLFKSYLKVAPQDTKLEKFYAVLGS
HQLSSVVETSVLLGNKLNSEDPVIAQRIGNIKSRIVERVKLFIEGTRERVKIRPNAMK
SLTIIPVSKIQLRRRLNASSIRSDVVSESIKAFMQQKVLYVDLEDFDWYDVSQALIRE
ILEKPSPDSIIVLELQLKSDLRSLQRKGFNVDRLMKQKMEEDIRLREQRQKAKEEARQ
KLQEQNRLKSEEAQRASDSNSEHPKSQALVPPTQPVPPSPQPMPDLISKSLPSQPEPT
TVDNVPGSFPSGERDPLIPQPSPEPQFQPLSQSSSLPIPQPKSSGEGRTGSKLLGGLF
NLTKKTEQLKPDYSKEPMDVLRKGLSSTKPFTQGKIKADMHVDVEPPKEMMSCDDGVA
QDLRLVTQLKGGLKCFVKSSEPNFTPVQLEEAARFQHILLACSKVSTTHPSLSKPRTT
PY"
gene <548349..>550058
/gene="MUP1"
/locus_tag="AWJ20_3880"
/db_xref="GeneID:30035950"
mRNA <548349..>550058
/gene="MUP1"
/locus_tag="AWJ20_3880"
/product="Mup1p"
/transcript_id="XM_018880918.1"
/db_xref="GeneID:30035950"
CDS 548349..550058
/gene="MUP1"
/locus_tag="AWJ20_3880"
/note="High affinity methionine permease; integral
membrane protein with 13 putative membrane-spanning
regions; also involved in cysteine uptake; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0005887 - integral component of plasma membrane
[Evidence ISS] [PMID 8893857]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA,IEA]; GO_function: GO:0000102 -
L-methionine secondary active transmembrane transporter
activity [Evidence IDA] [PMID 8893857]; GO_function:
GO:0015171 - amino acid transmembrane transporter activity
[Evidence IEA]; GO_process: GO:0003333 - amino acid
transmembrane transport [Evidence IEA]; GO_process:
GO:0006865 - amino acid transport [Evidence IEA];
GO_process: GO:0042883 - cysteine transport [Evidence IDA]
[PMID 11330701]; GO_process: GO:0044690 - methionine
import [Evidence IGI,IMP] [PMID 8893857]; GO_process:
GO:0015821 - methionine transport [Evidence IDA] [PMID
11330701]; GO_process: GO:0000101 - sulfur amino acid
transport [Evidence IMP] [PMID 8893857]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Mup1p"
/protein_id="XP_018733561.1"
/db_xref="GeneID:30035950"
/translation="MSAKYTRIDSGAGGSLGRPELGHGDSQISLELESLASSDEGSIR
SVSSRGLSPLDSPIRSPEIRDDDNDPMSYGMQFHGRSMSVSTMGEHMINLPSTNDPFL
GETAVPLEKQKRLTLFHGLSLVIGLQIGSGIFASPNQINSNAGSVGMSLIIWVAGGLL
AWTGASSYAELGSAIPLNGSSQAYLNQIYGSLPSFLFSWTALTILKPGSTAIIAIIFS
EYFCRALGSSPVFTNYWFQKFIAIIGLAIVVAINCFSTRLGARAGNFFLVIKIGVLLA
ITIIGIVASFHFHDTNALTGADSRGLFADSSKSIGNYAIALYAGLWAYDGWDNVNYVS
AEMKNPRRDLPRVIHIAMPVVIVAYFLANIAYYAVMSEEEIEKTTSVAITFATKTMGP
VAGIIFAILISLSCIGALNATIFSSARLVYSSAEEGFLPSMFCKLHPVRSTPVNALLL
QSAIACIFILVGEFHSLLTFYGFAGYSFYFLTVSGVIVLRIREPDLDRPYKTYISTPI
LFCCVALFLVSRTLFEKPIESLFVALFIASGLPIYIWRFGLLSQASVPSFDDILRKLK
IRR"
gene complement(<550308..>552002)
/locus_tag="AWJ20_3881"
/db_xref="GeneID:30035951"
mRNA complement(<550308..>552002)
/locus_tag="AWJ20_3881"
/product="hypothetical protein"
/transcript_id="XM_018880919.1"
/db_xref="GeneID:30035951"
CDS complement(550308..552002)
/locus_tag="AWJ20_3881"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733562.1"
/db_xref="GeneID:30035951"
/translation="MYRVNSTTVGAGTGHGMSPQPTGGGSARKVYVRQDRGADLGTGS
GLGSGIGINPDGNGMGGSLQSPLPAAVKSTGEIGSKGMFVRANSDTKVRPGSGSVKLK
AASVPVVATVSTSNSGIGIGAESVGGNGNVFSSNTPGSRASSVVSDRQPRPQLRQRVS
VLDLKPELHYNASVSSKSSVSSLNHNQVQSQNVNPSLDGRRSSNDSGSAGVNAYNKRL
MSPSVSGTNTPVISPTYKPLIGTKDSKVLKYSPTANVSAAAENLPSHVAVSSGANDQA
SDSFTPNLTESSTLASSSSSFPSLSSPSPSSSFASAPAGLLPSPSLHQGSTLPESSPK
DHSEILVKLPDKSANIISKAIQQESEQSSVSESMTLVPVLQPSDVTSLSAVSKARAER
KIKDLEITNTSLLSINRYLEKKIHRQKKDIERLSTRRTSDRSSMGRRTTRNTEHGSDG
DSDNDSDNESRYSGDYENSDLDESSLSSDFSDDEYSELDSSFIDTTLLDEVQERTSAH
ISFLKSAEKFDTMLRQCLIISDSLLNDAMSSLDFTVKPSDIGKKVVAVDAGESDDP"
gene complement(<552510..>553592)
/gene="ACT1"
/locus_tag="AWJ20_3882"
/db_xref="GeneID:30035952"
mRNA complement(<552510..>553592)
/gene="ACT1"
/locus_tag="AWJ20_3882"
/product="actin"
/transcript_id="XM_018880920.1"
/db_xref="GeneID:30035952"
CDS complement(552510..553592)
/gene="ACT1"
/locus_tag="AWJ20_3882"
/inference="similar to AA sequence:KEGG_Orthology:K05692"
/note="Actin; structural protein involved in cell
polarization, endocytosis, and other cytoskeletal
functions; GO_component: GO:0031011 - Ino80 complex
[Evidence IPI] [PMID 10952318]; GO_component: GO:0031011 -
Ino80 complex [Evidence IPI] [PMID 24034245];
GO_component: GO:0035267 - NuA4 histone acetyltransferase
complex [Evidence IDA] [PMID 10911987]; GO_component:
GO:0035267 - NuA4 histone acetyltransferase complex
[Evidence IPI] [PMID 15485911]; GO_component: GO:0000812 -
Swr1 complex [Evidence IDA] [PMID 14645854]; GO_component:
GO:0000812 - Swr1 complex [Evidence IDA] [PMID 14690608];
GO_component: GO:0000812 - Swr1 complex [Evidence IDA]
[PMID 16299513]; GO_component: GO:0030479 - actin cortical
patch [Evidence IDA] [PMID 3967297]; GO_component:
GO:0030479 - actin cortical patch [Evidence IDA] [PMID
6365930]; GO_component: GO:0030479 - actin cortical patch
[Evidence IDA] [PMID 8163554]; GO_component: GO:0005884 -
actin filament [Evidence IDA] [PMID 6217414];
GO_component: GO:0032432 - actin filament bundle [Evidence
IDA] [PMID 3967297]; GO_component: GO:0032432 - actin
filament bundle [Evidence IDA] [PMID 6365930];
GO_component: GO:0032432 - actin filament bundle [Evidence
IDA] [PMID 8163554]; GO_component: GO:0000142 - cellular
bud neck contractile ring [Evidence IDA] [PMID 3967297];
GO_component: GO:0000142 - cellular bud neck contractile
ring [Evidence IDA] [PMID 8163554]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005856 - cytoskeleton [Evidence IEA,IEA]; GO_function:
GO:0005524 - ATP binding [Evidence IEA]; GO_function:
GO:0004402 - histone acetyltransferase activity [Evidence
IDA] [PMID 10911987]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_function: GO:0005200 -
structural constituent of cytoskeleton [Evidence IDA]
[PMID 6217414]; GO_process: GO:0006281 - DNA repair
[Evidence IDA] [PMID 16135807]; GO_process: GO:0000916 -
actomyosin contractile ring contraction [Evidence IDA,IMP]
[PMID 9732290]; GO_process: GO:0030476 - ascospore wall
assembly [Evidence IDA] [PMID 17118118]; GO_process:
GO:0007119 - budding cell isotropic bud growth [Evidence
TAS] [PMID 10652251]; GO_process: GO:0034599 - cellular
response to oxidative stress [Evidence IGI] [PMID
17287397]; GO_process: GO:0001300 - chronological cell
aging [Evidence IMP] [PMID 15024029]; GO_process:
GO:0006897 - endocytosis [Evidence IMP] [PMID 8590801];
GO_process: GO:0030010 - establishment of cell polarity
[Evidence IGI] [PMID 9864365]; GO_process: GO:0000132 -
establishment of mitotic spindle orientation [Evidence
TAS] [PMID 10652251]; GO_process: GO:0006887 - exocytosis
[Evidence TAS] [PMID 10652251]; GO_process: GO:0031505 -
fungal-type cell wall organization [Evidence TAS] [PMID
10652251]; GO_process: GO:0016573 - histone acetylation
[Evidence IDA] [PMID 10911987]; GO_process: GO:0000001 -
mitochondrion inheritance [Evidence TAS] [PMID 10652251];
GO_process: GO:0009306 - protein secretion [Evidence
IGI,IMP] [PMID 10793147]; GO_process: GO:0000011 - vacuole
inheritance [Evidence IGI,IMP] [PMID 8978821]; GO_process:
GO:0030050 - vesicle transport along actin filament
[Evidence TAS] [PMID 10652251]"
/codon_start=1
/product="actin"
/protein_id="XP_018733563.1"
/db_xref="GeneID:30035952"
/translation="MCKAGFAGDDAPRAVFPSIVGRARHPGMMVGMNQKDSYVGDEAQ
SKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPVNPKSNRE
KMTQIVFETFNAPAFYVSIQAVLSLYASGRTTGIVLDSGDGVTHVVPIYAGFSLPHAI
MRINLAGRDLTDNLMKLLTERGYSFTTTAEREIVREIKEKLCYVALDFEQELQTASQS
SSLEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESPGIHETTFNSIMKCDVDVRK
DLYGNIVMSGGTTMYPGIAERMQKEITALAPSSMKIKIIAPPERKYSVWIGGSILASL
GTFQQMWISKQEYDESGPSIVHRKCF"
gene <559176..>559388
/locus_tag="AWJ20_3883"
/db_xref="GeneID:30035953"
mRNA <559176..>559388
/locus_tag="AWJ20_3883"
/product="hypothetical protein"
/transcript_id="XM_018880921.1"
/db_xref="GeneID:30035953"
CDS 559176..559388
/locus_tag="AWJ20_3883"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733564.1"
/db_xref="GeneID:30035953"
/translation="MAIPLPEKELTKSADNATGSNTSNLDGTAKEPYPTSIPTIIGNV
NISKEEAERLYEERIEDEYAKREGGA"
gene <562802..>566446
/gene="PMC1"
/locus_tag="AWJ20_3884"
/db_xref="GeneID:30035954"
mRNA <562802..>566446
/gene="PMC1"
/locus_tag="AWJ20_3884"
/product="calcium-transporting ATPase PMC1"
/transcript_id="XM_018880922.1"
/db_xref="GeneID:30035954"
CDS 562802..566446
/gene="PMC1"
/locus_tag="AWJ20_3884"
/inference="similar to AA sequence:KEGG_Orthology:K01537"
/note="Vacuolar Ca2+ ATPase involved in depleting cytosol
of Ca2+ ions; prevents growth inhibition by activation of
calcineurin in the presence of elevated concentrations of
calcium; similar to mammalian PMCA1a; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 24124599]; GO_component: GO:0000329 - fungal-type
vacuole membrane [Evidence IDA] [PMID 7507493];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005774 - vacuolar membrane [Evidence
IEA]; GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0005524 - ATP binding [Evidence IEA,IEA];
GO_function: GO:0005388 - calcium-transporting ATPase
activity [Evidence IEA,IEA]; GO_function: GO:0005388 -
calcium-transporting ATPase activity [Evidence IMP] [PMID
10431803]; GO_function: GO:0005388 - calcium-transporting
ATPase activity [Evidence IGI] [PMID 7507493];
GO_function: GO:0019829 - cation-transporting ATPase
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA,IEA];
GO_process: GO:0070588 - calcium ion transmembrane
transport [Evidence IEA]; GO_process: GO:0006816 - calcium
ion transport [Evidence IEA]; GO_process: GO:0006816 -
calcium ion transport [Evidence IMP] [PMID 15280017];
GO_process: GO:0006816 - calcium ion transport [Evidence
IMP] [PMID 7507493]; GO_process: GO:0006812 - cation
transport [Evidence IEA]; GO_process: GO:0006874 -
cellular calcium ion homeostasis [Evidence IMP] [PMID
7507493]; GO_process: GO:0006811 - ion transport [Evidence
IEA]; GO_process: GO:0008152 - metabolic process [Evidence
IEA]; GO_process: GO:0055085 - transmembrane transport
[Evidence IMP] [PMID 7507493]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="calcium-transporting ATPase PMC1"
/protein_id="XP_018733565.1"
/db_xref="GeneID:30035954"
/translation="MAAIATQEGKDWSADAKKILEEHREAEFEKKLPIGGGDVGFRYS
PDQLNSLVDPKNAFVLREMGGIEGLAGGLGVDLNTGLTDQQATDSTRLDVYGRNVLPE
RKTKSLLKLMWIALQDKVLIILSVAAVVSLALGIYETVGEPPEYDDQGHKLPKVDWVE
GVAILVAVVIVVTVGAVNDWQKERQFAKLNKKKEDRKVKAIRNGKPSEISVYDVVVGD
ILLVEPGEMIPTDGILVNGYNIKCDESSASGESDTMKKHPAKEVVDAIDHKSQGLNPQ
SKELDAFMLSGAKVLEGTGQFLTIAVGEKSLHGRTMMSLQDEPEATPLQEKLNNIAEG
IAKLGFAAAIVLFVALMIRFFVQLPGSVKTPSQKGEEFMTIFITAVTIVVVAVPEGLP
LAVTLALAFATTRMVKDNNLVRVLKSCETMGGATTICSDKTGTLTQNRMSVVAATVGF
KSCEASIDGPADDQEVPVGDKSLEKSHDKNYDTASEDVLSPSQLVGILPETQFTTLLD
SIALNSSAFEDNSPEATEPFLGSKTETALLGFARNYMNLGELLKYRQAADIVQVFPFD
SAKKFMATIVRTSSGSYQVLLKGASEVVLSRCTQILDTDSFTNTNLTAEHLEFINNQI
NLYASKSLRTIGLVYKDLPADWSPDAPYALEENTLFKDMTFLTLVGIKDPLRPGVKQA
VADCKTAGVVVRMVTGDNINTARAIALDCGILTSANNEDEIVMEGPEFRKLNPFELNS
VVPKLRVLARSSPQDKRKLVKFLKSLDETVAVTGDGTNDAPALKLADIGFSMGIAGTE
VAKEASDVILMDDNFGSIVKAIKWGRTVNDAVKKFLQFQLTVNVTAVLLTFISAVADS
DDTSVLTAVQLLWVNLIMDTFAALALATDPPSDSVLHRKPDNRKASLITVTMWKMILG
QAIFQLIVTFVLHFAGSQIFNVDTSVGRNKEQLSAMVFNTFVWLQFFNMFVNRRLDNH
QNIFEGVHRNIFFILIAAIIGGMQIVIMFVGGAAFSVVKQTGAMWGTALICGMISIPA
GVILRLVPDEWVAKLYPARAVNWAMSLLPRKKSSKGPEIIVDEESTMGDPQQYVWPAG
IEQVRRELVFLKTVRGGRVLQLNFKPKSIYNSWKEALTPSSASTNSINSPHSPSSTLA
PNSYFTDYHSNSNNGGNFLSVPGRRKGSNIAALAVVPAVVGGAIGGWSPELSTNPPPP
SSPDSPKNKK"
gene complement(<563746..>564084)
/locus_tag="AWJ20_3885"
/db_xref="GeneID:30035955"
mRNA complement(<563746..>564084)
/locus_tag="AWJ20_3885"
/product="hypothetical protein"
/transcript_id="XM_018880923.1"
/db_xref="GeneID:30035955"
CDS complement(563746..564084)
/locus_tag="AWJ20_3885"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733566.1"
/db_xref="GeneID:30035955"
/translation="MVVAPPIVSQDFNTRTRLLSLTIRVVANARARVTANGRPSGTAT
TTIVTAVMKIVINSSPFCEGVLTDPGNWTKKRIIRATNKTIAAAKPSLAIPSAILFSF
SCKGVASGSS"
gene complement(<564754..>565350)
/locus_tag="AWJ20_3886"
/db_xref="GeneID:30035956"
mRNA complement(<564754..>565350)
/locus_tag="AWJ20_3886"
/product="hypothetical protein"
/transcript_id="XM_018880924.1"
/db_xref="GeneID:30035956"
CDS complement(564754..565350)
/locus_tag="AWJ20_3886"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733567.1"
/db_xref="GeneID:30035956"
/translation="MNVNRTAVTLTVNWNWRNFLTASLTVRPHLMALTILPKLSSMRI
TSDASLATSVPAIPIEKPISASLSAGASLVPSPVTATVSSKDFRNLTSFRLSCGDDLA
RTRSLGTTLLSSKGLSFRNSGPSMTISSSLLAEVKIPQSRAMARAVLILSPVTIRTTT
PAVLQSATACLTPGRRGSLIPTRVKKVMSLNKVFSSSA"
gene complement(<567989..>569179)
/gene="YPS1"
/locus_tag="AWJ20_3887"
/db_xref="GeneID:30035957"
mRNA complement(<567989..>569179)
/gene="YPS1"
/locus_tag="AWJ20_3887"
/product="Yps1p"
/transcript_id="XM_018880925.1"
/db_xref="GeneID:30035957"
CDS complement(567989..569179)
/gene="YPS1"
/locus_tag="AWJ20_3887"
/note="Aspartic protease; member of the yapsin family of
proteases involved in cell wall growth and maintenance;
attached to the plasma membrane via a
glycosylphosphatidylinositol (GPI) anchor; YPS1 has a
paralog, MKC7, that arose from the whole genome
duplication; GO_component: GO:0031225 - anchored component
of membrane [Evidence IEA]; GO_component: GO:0009277 -
fungal-type cell wall [Evidence IDA] [PMID 10383953];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_function: GO:0004190 - aspartic-type
endopeptidase activity [Evidence IEA,IEA]; GO_function:
GO:0004190 - aspartic-type endopeptidase activity
[Evidence IDA] [PMID 8389368]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0008233
- peptidase activity [Evidence IEA]; GO_process:
GO:0031505 - fungal-type cell wall organization [Evidence
IGI] [PMID 16087741]; GO_process: GO:0016485 - protein
processing [Evidence IDA] [PMID 8626758]; GO_process:
GO:0006508 - proteolysis [Evidence IEA,IEA]"
/codon_start=1
/product="Yps1p"
/protein_id="XP_018733568.1"
/db_xref="GeneID:30035957"
/translation="MFFQAPVAILTLATLCAAAPANHKGYLSAKTKLHHVSHGNFTKR
SSSYETPLGNDIFAYVISFEVGTPPQPIEAAIDTGSADLWIYTTESGQTFPFDTSSSS
TYQVLNDDFQINYVSGSASGDWAIDTVNVAGASLTNQQFGSVSVNQNMGCVFGIGYQA
TEYTDTKYTNIPQNLVDQGYTAHNAYSLYLDDIDAGEGAILFGAVDSDKYSGQLYTVP
ITSSSSLQVTVSGVTGDNGDSLGGEVTAVLDSGTSLTYLPQDTADSIANYLGATFDSS
LGAYLLSDVPTSSITYSFSGALVTVPGSELAIPSSDFGFSNTQPYTLSIFPNSESADI
TLLGQSFLRSAYVVYDLDSNQISIAQSSWNSDSSNIHVITDSVPGAVSAPGLKNPINH
LHHQ"
gene <570186..>570258
/locus_tag="AWJ20_3888"
/db_xref="GeneID:30035958"
tRNA <570186..>570258
/locus_tag="AWJ20_3888"
/product="tRNA-Arg"
/db_xref="GeneID:30035958"
gene complement(<570577..>571596)
/locus_tag="AWJ20_3889"
/db_xref="GeneID:30035959"
mRNA complement(<570577..>571596)
/locus_tag="AWJ20_3889"
/product="carbonyl reductase (NADPH-dependent)"
/transcript_id="XM_018880926.1"
/db_xref="GeneID:30035959"
CDS complement(570577..571596)
/locus_tag="AWJ20_3889"
/note="Putative dihydrokaempferol 4-reductase;
GO_component: GO:0005575 - cellular_component [Evidence
ND]; GO_function: GO:0004090 - carbonyl reductase (NADPH)
activity [Evidence ISA] [PMID 19577617]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0050662 - coenzyme binding [Evidence IEA];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0044237 -
cellular metabolic process [Evidence IEA]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence IEA]"
/codon_start=1
/product="carbonyl reductase (NADPH-dependent)"
/protein_id="XP_018733569.1"
/db_xref="GeneID:30035959"
/translation="MVKVLLTGGSGFIAAHVLDILIEKGYEVVTTVRSQQKGQKILDA
HPKVSKDKLSYVIVEDIAQPGAFDEAVKSEPGFDAVIHTASPFHFNVVDPVKDLLDPA
VNGTVGILKAIKAHAPTVKRVAITSSFASIINASQHPETYSEKDWNPVTWEQATDPKD
PATTYRGSKTFAEKAAWDFVEKEKPNFTITTLCPPLVLGPIVHYLNSLDAINTSNARV
RDFIQGKYKGQSELPPTGTFIWVDVRDLALSHVLAIEKSEAENQRFFITAGYFSNKKI
VEAIRKSHPELSDKLPLSSAKDDTPEGVYGYDNSKSIKLLGLKYSPFDAAIKDTVDSL
LEVGA"
gene <573547..>574593
/gene="ATG3"
/locus_tag="AWJ20_3890"
/db_xref="GeneID:30035961"
mRNA <573547..>574593
/gene="ATG3"
/locus_tag="AWJ20_3890"
/product="Atg3p"
/transcript_id="XM_018880928.1"
/db_xref="GeneID:30035961"
CDS 573547..574593
/gene="ATG3"
/locus_tag="AWJ20_3890"
/inference="similar to AA sequence:KEGG_Orthology:K08343"
/note="E2-like enzyme; involved in autophagy and the
cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role
in formation of Atg8p-phosphatidylethanolamine conjugates,
which are involved in membrane dynamics during autophagy
and Cvt; interaction with Atg8p is regulated by its
acetylation by Esa1p (catalytic subunit of NuA4 histone
acetyltransferase complex) while attenuation of Atg3
acetylation is mediated by histone deacetylase Rpd3p;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005829 - cytosol [Evidence
IDA] [PMID 11149920]; GO_function: GO:0019776 - Atg8
ligase activity [Evidence IMP,ISS] [PMID 11100732];
GO_process: GO:0006501 - C-terminal protein lipidation
[Evidence IMP] [PMID 11100732]; GO_process: GO:0006501 -
C-terminal protein lipidation [Evidence IDA,IMP] [PMID
15277523]; GO_process: GO:0032258 - CVT pathway [Evidence
IMP] [PMID 9023185]; GO_process: GO:0000045 - autophagic
vacuole assembly [Evidence IMP] [PMID 11149920];
GO_process: GO:0006914 - autophagy [Evidence IEA];
GO_process: GO:0044805 - late nucleophagy [Evidence IMP]
[PMID 22768199]; GO_process: GO:0000422 - mitochondrion
degradation [Evidence IMP] [PMID 19793921]; GO_process:
GO:0034727 - piecemeal microautophagy of nucleus [Evidence
IMP] [PMID 18701704]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Atg3p"
/protein_id="XP_018733570.1"
/db_xref="GeneID:30035961"
/translation="MLRSTLSSFRDWREYLTPAYHVSSFKETGQITPEEFVEAGDYLV
YKFPTWSWAGAPASKRRDFLPEDKQVLVTKHVPSHLRAGAFTDGAGDELDVIVDGADG
GDGDDGWVSTAGSNHGSASLKSGSSKVIDREQTGSGPVTESDTASIEEIIDIDEFEEL
DEDDQIYRPDDGGDTSKSSGANNSTRSPNRTYNLFITYSTSYRVPKFYLSGFNADGNP
LSPNEMFEDIVVDYRDKTVTIEKAPFEEDLTLISVHPCRHANVMKILLDRAENRLRQK
QAVEHDELLKGISKVGLADSTVDDWEEIGSNEPPTTSELQQTAIRVDQYLVIFLKFIA
SVTPGIELDNTMVM"
gene <576039..>579665
/gene="PDS5"
/locus_tag="AWJ20_3891"
/db_xref="GeneID:30035962"
mRNA <576039..>579665
/gene="PDS5"
/locus_tag="AWJ20_3891"
/product="Pds5p"
/transcript_id="XM_018880929.1"
/db_xref="GeneID:30035962"
CDS 576039..579665
/gene="PDS5"
/locus_tag="AWJ20_3891"
/inference="similar to AA sequence:KEGG_Orthology:K11267"
/note="Protein involved in sister chromatid condensation
and cohesion; colocalizes with cohesin on chromosomes, may
function as a protein-protein interaction scaffold; also
required during meiosis; relocalizes to the cytosol in
response to hypoxia; GO_component: GO:0000794 - condensed
nuclear chromosome [Evidence IDA] [PMID 11062262];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
22932476]; GO_component: GO:0000798 - nuclear cohesin
complex [Evidence IDA] [PMID 22901742]; GO_component:
GO:0034990 - nuclear mitotic cohesin complex [Evidence
IDA] [PMID 22912589]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22842922]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 22932476]; GO_function:
GO:0005198 - structural molecule activity [Evidence ISS]
[PMID 11137006]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0051301 - cell division
[Evidence IEA]; GO_process: GO:0006302 - double-strand
break repair [Evidence IMP] [PMID 11448778]; GO_process:
GO:0006302 - double-strand break repair [Evidence IMP]
[PMID 19736318]; GO_process: GO:0007126 - meiotic nuclear
division [Evidence IMP] [PMID 15819623]; GO_process:
GO:0007076 - mitotic chromosome condensation [Evidence
IMP] [PMID 11062262]; GO_process: GO:0007067 - mitotic
nuclear division [Evidence IEA]; GO_process: GO:0007064 -
mitotic sister chromatid cohesion [Evidence IMP] [PMID
11062262]; GO_process: GO:0007064 - mitotic sister
chromatid cohesion [Evidence IMP] [PMID 14623866];
GO_process: GO:0007129 - synapsis [Evidence IMP] [PMID
19736318]"
/codon_start=1
/product="Pds5p"
/protein_id="XP_018733571.1"
/db_xref="GeneID:30035962"
/translation="MIESIFNIFYDVVQKEQIDGLEFLLTAILSQLINELETPPRPIT
KIVLSHLLRGEPQTNKDQEEEDGETNLPLAKKKKKPVAGRNKNDDQGNTVISFVSRGY
NVSKTVCMDNIDIMTRVVNQYVADILNPILLHTSNNSSSTGSEDLTEDIEKLGNLVIA
VWKAVPELLVNVLGQLEQNMSIENTALRYMSTRVIGEIICFEASRINFITEYNAIFKT
WIGRYRDVSPKIRINWANTVPNFVKNQTNHTIVLESVIPFLGLLEDPDEKVRIAVCKA
LANVDIDTIATTYATVVEQPLAALADRLRDKKSLVRLAAFQAVGKIYDEAYEQIAAGN
LQISDVSSWIPSHILGLLYVNDLEINEHLDWVLFDYIYPFEQLSGSDSEEAALARARR
LLTIVKALNEESFKAFTAISSRQVAVARYLNAYLEFCKQYHDAAGITRSGLKKSRQSN
GGNENSNENPSSPANRDELSNKINSLIKWVASVYPDSEKVHDHLTAFFKSNDRQAFKL
FGICINPDSDYKSVYDSYHSLMDRLSKSSSLNSIEPTIKSILYRSAFILFNKTNVSAI
INISRSATAQDSVNLKDVSYIILKEIAAIQPGLIKSQSQDISLIIENASPGDKGLVDT
LKTARLLFGKFPDLVPSAPAVWRSLLNIAKTGSIKEAKHATNVLDFAPKKNTLLTKLV
EGLDVILSGPYEDVPENFSTVLSCIAQLYYHNPQVIEPYSGLVISFLLRHVLLSNKAR
PAKDDDPDWVDDSEVNRGSLIERIEGPTSLEATGKLDEHLQRKLLALRIMVNRARSPY
LDEDSADEVAKPILNLLMTLLVHNGELNTKDEDTPPHYKSRLRLEACVKILKLSQTGH
CLKRIGPAELNVLGVLIRDSQLQVRDRFILKLTTLLSDGSIPERFLCLVFFVAGESNK
MLSNYCIRWIKARAARQVKSNSSSTALENSFVRFLHVLAHSGTGEEYEEGEDDNEQLL
TSLSRAAKYIIFFLKLIATESNISLLLYLSQRIKEYKDVVSQDSDSESSSSGLYTVAD
LAQICVLEMKKTRGWVPDPWPGKISLPADSFEPIESTEEAQRVFRAVYIPDNIDAQLR
ESISKEFHHHAKVKPEPTEASNGRGTSAPKKRTSQGSSSSSVKTKKPKAKAATKFKSL
TRASEESVAPRRSSRVATKKVNYMVENSDNEDEENEEEEEESNSEEDEEEEGSDDGND
TDQD"
gene complement(<579889..>580893)
/gene="RCO1"
/locus_tag="AWJ20_3892"
/db_xref="GeneID:30035963"
mRNA complement(<579889..>580893)
/gene="RCO1"
/locus_tag="AWJ20_3892"
/product="Rco1p"
/transcript_id="XM_018880930.1"
/db_xref="GeneID:30035963"
CDS complement(579889..580893)
/gene="RCO1"
/locus_tag="AWJ20_3892"
/note="Essential component of the Rpd3S histone
deacetylase complex; interacts with Eaf3p; GO_component:
GO:0032221 - Rpd3S complex [Evidence IDA] [PMID 16286007];
GO_component: GO:0032221 - Rpd3S complex [Evidence IDA]
[PMID 16286008]; GO_component: GO:0000118 - histone
deacetylase complex [Evidence IDA] [PMID 16286008];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0008270
- zinc ion binding [Evidence IEA]; GO_process: GO:0016568
- chromatin modification [Evidence IEA]; GO_process:
GO:0016575 - histone deacetylation [Evidence IMP] [PMID
16286007]; GO_process: GO:0060195 - negative regulation of
antisense RNA transcription [Evidence IMP] [PMID
21248844]; GO_process: GO:0030174 - regulation of
DNA-dependent DNA replication initiation [Evidence IMP]
[PMID 19417103]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006368 - transcription elongation from RNA polymerase
II promoter [Evidence IGI] [PMID 19948887]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA];
GO_process: GO:0061587 - transfer RNA gene-mediated
silencing [Evidence IMP] [PMID 23707796]"
/codon_start=1
/product="Rco1p"
/protein_id="XP_018733572.1"
/db_xref="GeneID:30035963"
/translation="MNQLGEYEDEIPKVNKPKATSKDENDPLKLLDKNGRPRMCYRCN
ESALRGTMVSCDVCPLNWHVDCLPSPTWTIGSRWKCPNHADQATKLPRRPRKYKVVDT
VLQRGFKNDGNVEVLDSSDEEEEGFVQDIPFFDNYDTRIGVASRINKGSEKTFLSNGV
LYRLPSRGIKLDFIEAVHDSYDEPYPDSIRSDILVALDELATKSLDVQQAVRNLAYLQ
SDGDPSVKVAIGKENINVLVSAALEELPDSNPAPQGPSVYIKPDTGSPSPPRHTNGRS
SARSSLAGSVTDSDRDALTSNSSVDDEVLDSERDQLRIIKRLMQIKGKDKLVKFLLEK
"
gene complement(<583659..>585271)
/gene="MAP2"
/locus_tag="AWJ20_3893"
/db_xref="GeneID:30035964"
mRNA complement(join(<583659..584782,585259..>585271))
/gene="MAP2"
/locus_tag="AWJ20_3893"
/product="Map2p"
/transcript_id="XM_018880931.1"
/db_xref="GeneID:30035964"
CDS complement(join(583659..584782,585259..585271))
/gene="MAP2"
/locus_tag="AWJ20_3893"
/note="Methionine aminopeptidase; catalyzes the
cotranslational removal of N-terminal methionine from
nascent polypeptides; function is partially redundant with
that of Map1p; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14562095]; GO_function:
GO:0004177 - aminopeptidase activity [Evidence IEA,IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA,IEA]; GO_function: GO:0070006 -
metalloaminopeptidase activity [Evidence IEA];
GO_function: GO:0070006 - metalloaminopeptidase activity
[Evidence IGI,ISA] [PMID 8618900]; GO_function: GO:0008235
- metalloexopeptidase activity [Evidence IEA];
GO_function: GO:0008233 - peptidase activity [Evidence
IEA]; GO_process: GO:0070084 - protein initiator
methionine removal [Evidence IEA]; GO_process: GO:0035551
- protein initiator methionine removal involved in protein
maturation [Evidence IMP] [PMID 11811952]; GO_process:
GO:0006508 - proteolysis [Evidence IEA,IEA]"
/codon_start=1
/product="Map2p"
/protein_id="XP_018733573.1"
/db_xref="GeneID:30035964"
/translation="MSTEPDYSLSNSDVVTKYKTAGDISAKVLANIKALAVEGAKIID
LTTEGDRQLLEETSKIYKGKKDIIKGIAFPTSVSPNNVVAHLSPLPTDKEAEITLKAG
DVVKITLGAQVDGFASIVGDTVVVGGGEVSGPVADAIAAAWYATEAAIRTIKPGNKNW
DVTKIVDKVTSEYGVKALEGMLSNEQSRNVVDGKKRIILNPSESQRREFETFTFEEGE
VYGVDILVSTGDGKSRADETPTTVYKKADITYQLKLRTSRATFSEVQKKAGSFPFSLR
SLDDAKKARMGLQECQNHGLIIPYEVFYEKEGQTVVQFFTTIALTKTGTIKFAGPVTP
DFAKIKTDKKVTDESVLDVLSKPLKASKKNKKKAADAAAAPASA"
gene <597377..>598729
/gene="ARG7"
/locus_tag="AWJ20_3894"
/db_xref="GeneID:30035965"
mRNA <597377..>598729
/gene="ARG7"
/locus_tag="AWJ20_3894"
/product="glutamate N-acetyltransferase"
/transcript_id="XM_018880932.1"
/db_xref="GeneID:30035965"
CDS 597377..598729
/gene="ARG7"
/locus_tag="AWJ20_3894"
/inference="similar to AA sequence:KEGG_Orthology:K00620"
/note="Mitochondrial ornithine acetyltransferase;
catalyzes the fifth step in arginine biosynthesis; also
possesses acetylglutamate synthase activity, regenerates
acetylglutamate while forming ornithine; GO_component:
GO:0005759 - mitochondrial matrix [Evidence IEA,IEA];
GO_component: GO:0005759 - mitochondrial matrix [Evidence
IDA] [PMID 205532]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0004042 - acetyl-CoA:L-glutamate N-acetyltransferase
activity [Evidence IEA,IEA]; GO_function: GO:0004042 -
acetyl-CoA:L-glutamate N-acetyltransferase activity
[Evidence IDA,IGI] [PMID 9428669]; GO_function: GO:0003824
- catalytic activity [Evidence IEA]; GO_function:
GO:0004358 - glutamate N-acetyltransferase activity
[Evidence IEA,IEA,IEA]; GO_function: GO:0004358 -
glutamate N-acetyltransferase activity [Evidence IDA]
[PMID 9428669]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_function: GO:0016746 -
transferase activity, transferring acyl groups [Evidence
IEA]; GO_process: GO:0006526 - arginine biosynthetic
process [Evidence IEA,IEA,IEA,IEA]; GO_process: GO:0006526
- arginine biosynthetic process [Evidence TAS] [PMID
12603335]; GO_process: GO:0008652 - cellular amino acid
biosynthetic process [Evidence IEA]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0006592 - ornithine biosynthetic process [Evidence IDA]
[PMID 9428669]"
/codon_start=1
/product="glutamate N-acetyltransferase"
/protein_id="XP_018733574.1"
/db_xref="GeneID:30035965"
/translation="MLSAVRSGRLIQRLAVGGRGPRQFSTSTVALVADKSKYVPSSGI
YPQGYKVGAIHCGVKKNAEALDLSVLVSETPCSGAAVFTTNIFQAAPVQVSKKVLSST
KGTGIRSIIVNSGCANAVTGEGGLKDAEAMVSTTDRVVGVTSGPASLVMSTGVIGQRL
PIDKILNGIPSLIESNLGTDHNAWVTCARGICTTDTFPKLTSKEFSIGSNTYRIAGLA
KGAGMIHPNMATLLGFFVTDAPVSQDALHDALKYATDRSFNSISVDGDMSTNDTIAAL
ANGAAGGPVITKDSDSYEVLRSVITEFAEELAQLVVRDGEGATKFVTIKVEDALNFAD
AKQVASSIATSSLVKTALYGKDANWGRILCATGYAGVPVEPFKTSVSFIPTDGTAELK
LLVNGEPEQVDEERASEILALEDLEIKVSLGTGGGASAKFWTCDLSHEYVTINGDYRS
"
gene complement(<597652..>598059)
/locus_tag="AWJ20_3895"
/db_xref="GeneID:30035966"
mRNA complement(<597652..>598059)
/locus_tag="AWJ20_3895"
/product="Ecm40p"
/transcript_id="XM_018880933.1"
/db_xref="GeneID:30035966"
CDS complement(597652..598059)
/locus_tag="AWJ20_3895"
/codon_start=1
/product="Ecm40p"
/protein_id="XP_018733575.1"
/db_xref="GeneID:30035966"
/translation="MLGWIIPAPLARPAMRYVLDPMENSLDVSLGNVSVVQMPRAQVT
HALWSVPRFDSMSDGMPFRILSIGNLWPITPVDMTNEAGPDVTPTTLSVVETMASASF
KPPSPVTALAQPLLTIIDRIPVPLVLDRTFLET"
gene complement(<602256..>604001)
/locus_tag="AWJ20_3896"
/db_xref="GeneID:30035967"
mRNA complement(<602256..>604001)
/locus_tag="AWJ20_3896"
/product="hypothetical protein"
/transcript_id="XM_018880934.1"
/db_xref="GeneID:30035967"
CDS complement(602256..604001)
/locus_tag="AWJ20_3896"
/inference="similar to AA sequence:KEGG_Orthology:K15029"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733576.1"
/db_xref="GeneID:30035967"
/translation="MEDYEYDDFEAGNETIAVGEHVYDEAALLRQQEALMEAARKAAY
VPEAIKEFILYFQKCLHEKNVYELLSCYDNSFRKLTEKFYKNLPWPSPELAVAPIVNN
DQTFLLFYKELYYRHLYAKGQPTIEQRLDSYHNYCTLFDFIINSETPTDIELPTQWCW
DMIDEFIYQFNSFSIFRSRLIKKGNASQADLELLEKNVGVWGAYPVLNVLYSLAGKAN
ITAQLKATKNGQDATQVAGPFGSKPLYNTLGYFSLIGLLRVHTLLGDYALALKTLEDV
QLNKRALLSRVTGAHYTTYYYVGFCYLMLRRYADAIKSFSHILVFISRTKNINRNAQF
DNVNKKSDQMYALLAICVALCPTRLDDIIHTGMREKYGEQLSKMNRGGPDALKTFEEL
FVFAAPKFISPTPPDFEHPEYNVEPLQHHLKIFLETVKTANLGYTLKNYLNLYATMDI
RKLSGFLETDVELLRSALVSFKIKNKQTKWTEGALLDGESVNVSEIDIALDNDLIHIA
ESKDGRKFADWFIRNTLKNYTAQEFIANPDSKQDQQHDKSKKQHNRPKKDTNNTKKDS
TPAPSTSSSAPVAAK"
gene complement(<605282..>605665)
/gene="SNU13"
/locus_tag="AWJ20_3897"
/db_xref="GeneID:30035968"
mRNA complement(<605282..>605665)
/gene="SNU13"
/locus_tag="AWJ20_3897"
/product="RNA binding protein SNU13"
/transcript_id="XM_018880935.1"
/db_xref="GeneID:30035968"
CDS complement(605282..605665)
/gene="SNU13"
/locus_tag="AWJ20_3897"
/inference="similar to AA sequence:KEGG_Orthology:K12845"
/note="RNA binding protein; part of U3 snoRNP involved in
rRNA processing, part of U4/U6-U5 tri-snRNP involved in
mRNA splicing, similar to human 15.5K protein;
GO_component: GO:0046540 - U4/U6 x U5 tri-snRNP complex
[Evidence IDA] [PMID 10377396]; GO_component: GO:0046540 -
U4/U6 x U5 tri-snRNP complex [Evidence IDA] [PMID
10449419]; GO_component: GO:0031428 - box C/D snoRNP
complex [Evidence IDA] [PMID 11081632]; GO_component:
GO:0005730 - nucleolus [Evidence IEA,IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 10684247];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA,IEA]; GO_component: GO:0032040 -
small-subunit processome [Evidence IDA] [PMID 12068309];
GO_component: GO:0005681 - spliceosomal complex [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence
IEA,IEA]; GO_function: GO:0003723 - RNA binding [Evidence
IMP,ISS] [PMID 14730029]; GO_function: GO:0003723 - RNA
binding [Evidence IDA] [PMID 17332014]; GO_function:
GO:0003723 - RNA binding [Evidence IPI] [PMID 17631273];
GO_process: GO:0008380 - RNA splicing [Evidence IEA];
GO_process: GO:0006397 - mRNA processing [Evidence IEA];
GO_process: GO:0000398 - mRNA splicing, via spliceosome
[Evidence IPI] [PMID 10377396]; GO_process: GO:0000398 -
mRNA splicing, via spliceosome [Evidence IMP] [PMID
11720284]; GO_process: GO:0000398 - mRNA splicing, via
spliceosome [Evidence IMP] [PMID 14730029]; GO_process:
GO:0000462 - maturation of SSU-rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 14730029]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA,IEA]"
/codon_start=1
/product="RNA binding protein SNU13"
/protein_id="XP_018733577.1"
/db_xref="GeneID:30035968"
/translation="MSTNADPRAFPLASSDVTQQILDIVQQATHLRQLKKGANEATKT
LNRGISEFIVMAADASPIEIVLHLPLLCEDKNVPYVFVPSKIALGRACGVSRPVIAAS
VTSNDASQLRDQINGIKDVIERLLI"
gene <606664..>608376
/locus_tag="AWJ20_3898"
/db_xref="GeneID:30035969"
mRNA <606664..>608376
/locus_tag="AWJ20_3898"
/product="hypothetical protein"
/transcript_id="XM_018880936.1"
/db_xref="GeneID:30035969"
CDS 606664..608376
/locus_tag="AWJ20_3898"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733578.1"
/db_xref="GeneID:30035969"
/translation="MDSSARLTAYTIRYVLSPREYRKLVARLNTNVTRILPAPEKIDA
SAVRSGDGVQSIVRSQSRLFAASYGLLGLINAFILKRRGLNIPALRIATSLTAISGIY
KVLYRVLTLLLQRTGILPRHGLSAKTTGSSTKSRVDSSPSPGTTDDPESTCSDAGYLD
NSYKITKLLVRAGYFTKRTLKKTKHLPPLLPALLSGLVAGSAFALFPESAQGYAAIYL
STRTLEFVYNYLDDMGYLEFKPRILKSWAIFPFAFSQLFYTFIFHPDACPNIFRRIML
RLSDGYIPTKPTGYPIDRPWPNPDQVVDGIATIARARYPKFVSPILFPNLDTTVPGLE
SAEPVLSLAHPAMSTLTGALSHPTQPSEFRTYTELVLSKYSSIYKYVFGAYVVLGLLK
WRAHSKASSKTSEKNKLSSTDKASKKAAPSLPGILLSSVANTARTTTFIVMTAASSWY
GIGLSQQVLNNKFIPVLRFRIIGFLAGLWAFADQVNGRARYMFAVRLALRSQWNVLSK
DRRYAPLLKNGAAALFALSFAVLMAIFDLSPKSIPGPSLRKTLNWIKTNQYTDVTAEK
EKDI"
gene <613092..>614648
/gene="APE1"
/locus_tag="AWJ20_3899"
/db_xref="GeneID:30035970"
mRNA <613092..>614648
/gene="APE1"
/locus_tag="AWJ20_3899"
/product="Ape1p"
/transcript_id="XM_018880937.1"
/db_xref="GeneID:30035970"
CDS 613092..614648
/gene="APE1"
/locus_tag="AWJ20_3899"
/inference="similar to AA sequence:KEGG_Orthology:K01268"
/note="Vacuolar aminopeptidase yscI; zinc
metalloproteinase that belongs to the peptidase family
M18; often used as a marker protein in studies of
autophagy and cytosol to vacuole targeting (CVT) pathway;
protein increases in abundance and relative distribution
to cytoplasmic foci increases upon DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
22842922]; GO_component: GO:0000324 - fungal-type vacuole
[Evidence IDA] [PMID 1400574]; GO_component: GO:0005773 -
vacuole [Evidence IEA,IEA]; GO_function: GO:0004177 -
aminopeptidase activity [Evidence IEA,IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0070006 - metalloaminopeptidase
activity [Evidence IDA] [PMID 3882418]; GO_function:
GO:0008237 - metallopeptidase activity [Evidence IEA];
GO_function: GO:0008233 - peptidase activity [Evidence
IEA]; GO_function: GO:0008270 - zinc ion binding [Evidence
IEA]; GO_process: GO:0006508 - proteolysis [Evidence
IEA,IEA]; GO_process: GO:0007039 - vacuolar protein
catabolic process [Evidence ISS] [PMID 2651436];
GO_process: GO:0007039 - vacuolar protein catabolic
process [Evidence ISS] [PMID 2689224]"
/codon_start=1
/product="Ape1p"
/protein_id="XP_018733579.1"
/db_xref="GeneID:30035970"
/translation="MTSFRANPAFKNARQRKASSPESFEIVDSVSTNGNGSSESEKAT
IRGEFDPKLSSTQYTDLFLDFMNTSPTTYHAVNSVAEVLEAQGFVYLSERESWEDKIT
KSNRFYTTRNGSSLLAFVVGKDWAPGKGAAIIGSHIDALTGKVKPISKKTPVEGYEQI
GTAPYSGAFNSTWWDRDLGIGGRIITRSKDTKKIESKLVRIPYPVARIPTLAPHFGAV
ASGPFNPETQMTPIIGLIGDEDPELVATADEKRSPLIGKHSLRLLRALSKHSGIPVGD
FLQLDLEVYDTHPGTVGGLDKEFVFCPRIDDKLCSFAAVYGLLESLEKVDDNSSLSFV
ALYDDEEIGSLLRQGAESNLLEGTIDRLLALHGSSDEELKRLTYANSFLISADVIHAV
NPNFDNVYLEHHKPKLNVGVTVSFDPNGHMTTDAVSTAFVEEIARRTDNVLQAFQIRN
DSRSGGTIGPKLSSKTGLRAIDMGIPQLSMHSIRATTGSKDVYLGVKFFKAFFEKWEE
VDVLFKLGDL"
gene complement(<614915..>616768)
/gene="AMF1"
/locus_tag="AWJ20_3900"
/db_xref="GeneID:30035973"
mRNA complement(<614915..>616768)
/gene="AMF1"
/locus_tag="AWJ20_3900"
/product="Amf1p"
/transcript_id="XM_018880940.1"
/db_xref="GeneID:30035973"
CDS complement(614915..616768)
/gene="AMF1"
/locus_tag="AWJ20_3900"
/note="Putative paralog of ATR1; but not required for
boron tolerance; member of the DHA2 family of drug:H+
antiporters; YOR378W is not an essential gene;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0008150 - biological_process
[Evidence ND]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Amf1p"
/protein_id="XP_018733580.1"
/db_xref="GeneID:30035973"
/translation="MTQLLEKKNGDVESLLQKEAASLDKTGSTGSGVESETQFDHQKT
SKELVGGDGGGLENGLDHRLEHGMDSNLDSDPHGLDHEPDGGDSSEGGDEGSNGPESH
RPNISYSREYLLILVIISAQLMTQASVAQGLSIGREIGLHFNTTDAGQLSWYVAGFSL
TAGTFIVSSGRLGEIYGHKNLFVIGYAWLFVFTLIAGISSYSNSSIFFSVCRGLQGLG
CAILLPNGLAIFGMLYPAGSRKNIVFALFGAVAPGGFVLGAIFSTLLAKHRWDFTFYV
GSIVCFLLTIAAYVIIPNYENKSNHDRQPFDILGALTGIGGLMLFNVAWNQGPVVGWS
DPYVYILLILGVLLFVAFGIVETRVSHPLVPVSTMKSSTILILTAVACGWACFGIWLY
YTWQLFTVLRQQTPLLAAVEMLPALFGGFLASGAAAFLFPRIPVSVLMAMALVGFCVP
SILLATNRVHETYWAASFVATVIAPFGKCFGDLPPAAWALPQTPLCSASQSGLDCGGS
NNCVFYVSNFVAGMDISFPAATILLSLSVPKESQGIAASLVATVVNYAISIGLGIAGT
VVRYTTPGTDEQSLLNAVRYSAYLGIGLSSLGLIFALIAVWQDRKNLSRPH"
gene <619297..>620592
/gene="DAL5"
/locus_tag="AWJ20_3901"
/db_xref="GeneID:30035974"
mRNA <619297..>620592
/gene="DAL5"
/locus_tag="AWJ20_3901"
/product="allantoate permease"
/transcript_id="XM_018880941.1"
/db_xref="GeneID:30035974"
CDS 619297..620592
/gene="DAL5"
/locus_tag="AWJ20_3901"
/note="Allantoate permease; ureidosuccinate permease; also
transports dipeptides, though with lower affinity than for
allantoate and ureidosuccinate; expression is constitutive
but sensitive to nitrogen catabolite repression;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0015124 -
allantoate transmembrane transporter activity [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0042936 -
dipeptide transporter activity [Evidence IMP] [PMID
16429164]; GO_function: GO:0042936 - dipeptide transporter
activity [Evidence IGI,IMP] [PMID 17693598]; GO_function:
GO:0005215 - transporter activity [Evidence IEA];
GO_process: GO:0015719 - allantoate transport [Evidence
IMP] [PMID 3275614]; GO_process: GO:0042938 - dipeptide
transport [Evidence IMP] [PMID 16429164]; GO_process:
GO:0042938 - dipeptide transport [Evidence IGI,IMP] [PMID
17693598]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA,IEA]; GO_process: GO:0042939 -
tripeptide transport [Evidence IMP] [PMID 16429164]"
/codon_start=1
/product="allantoate permease"
/protein_id="XP_018733581.1"
/db_xref="GeneID:30035974"
/translation="MGFKEDLNLVGNDFSFLGMFFYVGFLAAELPTQWLAQRISFLGK
YLGINVTLWGVVLACHAACNSYAQVAVLRTLLGIFEACVAPILVLIICMWYKKQEQGK
RVACFYVMNSFTSVAAGLIAYGASFTKSSFASWRIFLLCMGLVTVVCGVLVFLYLPDS
PTKAKGFTDEEKLAVLLRIKDDQSGTQNNKLKGYQMLEAVSDPKVWLVVLSVMLISIP
NGALSIFSSLIVNSFGFSAQETLIVGVPGGLLSGFWLLAVQYYSDKTQNRTLMNLCYL
LPAILGLALMVGLENREKTTAMKTGLLISNYLSQTFGGTLALILSWNASNVAGHSKKV
LVNALTFIAFPLGNIIGTQTFQAKDAPMYMPGKAAILGCLSLQCFITVGWYFLNQFYN
NKKQRVLDEMDPEQRETMRRQMEFSDETDMRNPFFRYTK"
gene <623341..>626019
/gene="PAT1"
/locus_tag="AWJ20_3902"
/db_xref="GeneID:30035975"
mRNA <623341..>626019
/gene="PAT1"
/locus_tag="AWJ20_3902"
/product="Pat1p"
/transcript_id="XM_018880942.1"
/db_xref="GeneID:30035975"
CDS 623341..626019
/gene="PAT1"
/locus_tag="AWJ20_3902"
/inference="similar to AA sequence:KEGG_Orthology:K12617"
/note="Deadenylation-dependent mRNA-decapping factor; also
required for faithful chromosome transmission, maintenance
of rDNA locus stability, and protection of mRNA 3'-UTRs
from trimming; associated with topoisomerase II; binds to
mRNAs under glucose starvation, most often in the 3' UTR;
functionally linked to Pab1p; forms cytoplasmic foci upon
DNA replication stress; phosphorylation by PKA inhibits P
body foci formation; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 22842922]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 23706738]; GO_component:
GO:0000932 - cytoplasmic mRNA processing body [Evidence
IEA]; GO_component: GO:0000932 - cytoplasmic mRNA
processing body [Evidence IDA,IMP] [PMID 12730603];
GO_component: GO:0010494 - cytoplasmic stress granule
[Evidence IDA] [PMID 23222640]; GO_component: GO:0022627 -
cytosolic small ribosomal subunit [Evidence IDA] [PMID
10779343]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 23706738]; GO_function: GO:0003723 - RNA
binding [Evidence IEA]; GO_function: GO:0003682 -
chromatin binding [Evidence IDA] [PMID 23706738];
GO_function: GO:0003729 - mRNA binding [Evidence IDA,IDA]
[PMID 23222640]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0051301 - cell division
[Evidence IEA]; GO_process: GO:0033962 - cytoplasmic mRNA
processing body assembly [Evidence IGI,IMP] [PMID
18981231]; GO_process: GO:0000290 -
deadenylation-dependent decapping of nuclear-transcribed
mRNA [Evidence IMP,IPI] [PMID 10913177]; GO_process:
GO:0001731 - formation of translation preinitiation
complex [Evidence IMP] [PMID 20832728]; GO_process:
GO:0006397 - mRNA processing [Evidence IEA]; GO_process:
GO:0045947 - negative regulation of translational
initiation [Evidence IDA,IMP] [PMID 20832728]; GO_process:
GO:0006417 - regulation of translation [Evidence IEA];
GO_process: GO:0006446 - regulation of translational
initiation [Evidence IGI,IMP] [PMID 10779343]"
/codon_start=1
/product="Pat1p"
/protein_id="XP_018733582.1"
/db_xref="GeneID:30035975"
/translation="MSFFGVKTASSGGEAAVYEFDDTYDDNLGAALEETNDDFNDETF
GDFGDGVGNDFDFAGQTANVADTIDEEQFTFARNAGGKLGSVSGAGGAGGSGLNLLPS
TTGSVPSLQPIASLWGTAGVDKKPEPQPAAQQQQPQQPQQPKILSLEEVEAQMLGKSQ
QQLPHGVLPQPPQGFPPQGFPPQGFFPQGFPPQGFPPQGFPGQGFPQVPQGYPPQGFP
PQGYPLPQDPAVLMSSGYYGSGAGPTPGQPGLGQPGQPGQPPHQQQIPGQQQLQQQQQ
QPQQQQQAQFQQPPAGQSVQADSAIKQIEGQLAQTSVQDQGQSQGQRPQGAHGQEANA
QNNAQGNAQTPYSLPQQVQQGQQPVVPLNQVINEDLAQSEAEHQRMFEKSRKIAEIVK
YNGLMSQWDKNFIMRIQLQQMVTADPYNEDFYYQVHSAIQARNNPQQPLNEFARTYLL
QRGQRGNGRFRRHNDNPLQRMQQQVQRAVAAAKEHPKKEQIAPEGALGKISVGGGKQP
RKALNVAGSASNIPSSPSTSSSAKVEALKKLTLTSSTSQSGTYTKKGLLRSIENVYTI
LLEIESVERSRPSEDFNDQQQQQHQQQAAEDVADSDSSPAATPASVVSYEERLQTWQD
KMQTLVDQLWNELQVLEPIDSANNQPFILMLSHDKGKKVIPRIFRHLSQKQRLTVLTR
IVAHIESLDVVKDGDYGSSGTLSAKTRESIELFSQTVLPPLVHLVSESSYDVVIGLLE
IMLNSSNMVHVSSTKIGLAFLTVLISRAELISQEESEAATPSAAATRDLANWQATFNT
LFTKVQGHLSSIFPPRNVDNSYVWHFLASLALAAKLEHQRIIVDEVREMIFGTMNEAK
ALPVELGVSKIANLNLFLNVMGLNATTTEITELSSQ"
gene <626975..>627202
/gene="ACP1"
/locus_tag="AWJ20_3903"
/db_xref="GeneID:30035976"
mRNA <626975..>627202
/gene="ACP1"
/locus_tag="AWJ20_3903"
/product="Acp1p"
/transcript_id="XM_018880943.1"
/db_xref="GeneID:30035976"
CDS 626975..627202
/gene="ACP1"
/locus_tag="AWJ20_3903"
/inference="similar to AA sequence:KEGG_Orthology:K03955"
/note="Mitochondrial matrix acyl carrier protein; involved
in biosynthesis of octanoate, which is a precursor to
lipoic acid; activated by phosphopantetheinylation
catalyzed by Ppt2p; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0070469 - respiratory
chain [Evidence IEA]; GO_function: GO:0000036 - ACP
phosphopantetheine attachment site binding involved in
fatty acid biosynthetic process [Evidence ISS] [PMID
8394042]; GO_process: GO:0006633 - fatty acid biosynthetic
process [Evidence IEA,IEA,IEA]; GO_process: GO:0006631 -
fatty acid metabolic process [Evidence IEA]; GO_process:
GO:0006629 - lipid metabolic process [Evidence IEA];
GO_process: GO:0009107 - lipoate biosynthetic process
[Evidence IMP] [PMID 9187370]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]"
/codon_start=1
/product="Acp1p"
/protein_id="XP_018733583.1"
/db_xref="GeneID:30035976"
/translation="MARSIETNMLQIADPSKITPSANFSTDLGLDSLDVVDALFFIEE
EFNIEIPDHEADEIHTVAQAVDYILAQPDGK"
gene <629147..>630802
/gene="PCS60"
/locus_tag="AWJ20_3904"
/db_xref="GeneID:30035977"
mRNA <629147..>630802
/gene="PCS60"
/locus_tag="AWJ20_3904"
/product="Pcs60p"
/transcript_id="XM_018880944.1"
/db_xref="GeneID:30035977"
CDS 629147..630802
/gene="PCS60"
/locus_tag="AWJ20_3904"
/note="Oxalyl-CoA synthetase; capable of catalyzing
conversion of oxalate to oxalyl-CoA; catalyzes first step
in pathway of oxalate degradation that functions to
protect yeast from inhibitory effects of oxalate;
peroxisomal protein that binds mRNA; localizes to both
peroxisomal peripheral membrane and matrix, expression is
highly inducible by oleic acid; similar to E. coli long
chain acyl-CoA synthetase; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11914276]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005782 - peroxisomal
matrix [Evidence IEA]; GO_component: GO:0005782 -
peroxisomal matrix [Evidence IDA] [PMID 8841414];
GO_component: GO:0005778 - peroxisomal membrane [Evidence
IEA]; GO_component: GO:0005778 - peroxisomal membrane
[Evidence IDA] [PMID 8841414]; GO_component: GO:0005777 -
peroxisome [Evidence IEA]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0016874
- ligase activity [Evidence IEA]; GO_function: GO:0003729
- mRNA binding [Evidence IDA] [PMID 20844764];
GO_function: GO:0050203 - oxalate-CoA ligase activity
[Evidence IDA,IMP] [PMID 24291261]; GO_process: GO:0008152
- metabolic process [Evidence IEA]; GO_process: GO:0033611
- oxalate catabolic process [Evidence IDA,IMP] [PMID
24291261]"
/codon_start=1
/product="Pcs60p"
/protein_id="XP_018733584.1"
/db_xref="GeneID:30035977"
/translation="MSTSSSTPSSPPVIHKSPWGEVPDLFVGNIAQFVRESPHINRKL
DKKILIDAKTNQAIRGRQLFNFTARLSWVLRTKYKIEYDDVVCLFAPNSVWTPIIHHG
VLSRGAILSPANIAYNPEELHYQIKTANAKLALVGDAHLETARKALKVGPNNCVKIVP
ISRLIKDIKFAKGVENPVPLEGDVAKDKIAYLCFSSGTSGTPKGVMTTHYNMTSNAQQ
QFYCFNKALLNGNNVYGAVLPMSHIFGLTKFVYSTLYVASTIVVFEKFDFPLLLSSIL
KYNINVLHVVPPILVLFAKSPLVDKYAIKGRLNHIFTGAAPAGKEIIALAEARTGAHI
SQGYGLTESSPVTHLYSYDEQAYDQNSIGWLIASCEARLVTDEGTDAAVGERGELWIR
GPNIMKGYLNNPAATAETLTSDGFLKTGDVAIRVADGQYYIVDRKKELIKSKGHQVAP
AELEALLLDHPDVVDVAVVGVHVSELGTELPRAFLVIREKTDPLEIKKWFDSKVARHK
RLWGGIVVLDAIPKSPSGKILRRLLRDRKDDKAIGFTLAEPKL"
gene complement(<631041..>632960)
/gene="DBP6"
/locus_tag="AWJ20_3905"
/db_xref="GeneID:30035978"
mRNA complement(<631041..>632960)
/gene="DBP6"
/locus_tag="AWJ20_3905"
/product="Dbp6p"
/transcript_id="XM_018880945.1"
/db_xref="GeneID:30035978"
CDS complement(631041..632960)
/gene="DBP6"
/locus_tag="AWJ20_3905"
/inference="similar to AA sequence:KEGG_Orthology:K14807"
/note="Essential protein involved in ribosome biogenesis;
putative ATP-dependent RNA helicase of the DEAD-box
protein family; GO_component: GO:0005730 - nucleolus
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 9528757]; GO_component: GO:0005634 -
nucleus [Evidence IEA]; GO_component: GO:0030687 -
preribosome, large subunit precursor [Evidence IDA] [PMID
15126390]; GO_component: GO:0030687 - preribosome, large
subunit precursor [Evidence IDA] [PMID 17145778];
GO_function: GO:0005524 - ATP binding [Evidence IEA,IEA];
GO_function: GO:0004004 - ATP-dependent RNA helicase
activity [Evidence ISS] [PMID 10322435]; GO_function:
GO:0008026 - ATP-dependent helicase activity [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence
IEA]; GO_function: GO:0004386 - helicase activity
[Evidence IEA,IEA]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0003676 - nucleic
acid binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006200
- ATP catabolic process [Evidence IEA]; GO_process:
GO:0000466 - maturation of 5.8S rRNA from tricistronic
rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 9528757]; GO_process: GO:0000463 - maturation
of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID 9528757];
GO_process: GO:0006364 - rRNA processing [Evidence IEA];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA]"
/codon_start=1
/product="Dbp6p"
/protein_id="XP_018733585.1"
/db_xref="GeneID:30035978"
/translation="MFLATKRFDPNFTSQREEDDQPEPKKVKLDKELIKKDVVPSSLS
SENNPAKSNTPIETNSLDSSHVSSSKSVLEESDDSESEYEVDPKYSAVFKRFQDIASK
AAKNNVRTSRLADQEAISKDSENNQEEPDEEEIPEVNELVPLPQIARKKESSKKHKEP
PKWITEPIIIPLNASKPFAEYEKEHLLSARMVKVLQSLNYESAFAVQTGVCPVLLKDS
QSIAPDPLPDVLVNAYTGSGKTLAYGIPIVEALSKRVVPRIRALILLPTRPLIQQVRN
VLESLAKGTSLRVMICRTDRPFKEEQALISSHTPDILITTPGRLVDHIRNNTQGFSLN
HLQYLVVDEADRLLNQSFQEWVSVLVNALEAAKPKESLAKVWNRPPQKLIFSATLTRD
PGKLAALNITTTPTPRIFVLGEEPVVNNGHTNTTSGPSVHEDRQRIMMDYEFSLPEKL
QEHHIQVKSASNKPLVLLQLLINHSLHSDTLIFVKSNQAAARLARLIQLIDEELFHLN
LSVERCSGELELSQRRKMLKKFADGEIGILVCTDLIARGIDIASVKAVINYDLPVGKR
EYVHRVGRTARAGNSGAAWNLTVGSGERKFFNSITTSIFRTGEIESEVIKPSPDHNDG
YQKALSRLEQEVFAK"
gene <635645..>637453
/gene="MSS4"
/locus_tag="AWJ20_3906"
/db_xref="GeneID:30035979"
mRNA <635645..>637453
/gene="MSS4"
/locus_tag="AWJ20_3906"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018880946.1"
/db_xref="GeneID:30035979"
CDS 635645..637453
/gene="MSS4"
/locus_tag="AWJ20_3906"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733586.1"
/db_xref="GeneID:30035979"
/translation="MGAFFFSNGDYLPSNGTHKVPRRPVLSDVHCGVSVPKYGACAQL
ENRHPARRSAFAYHKVLILECSDDSGVSVGSDETLNEPTVWKPVDIVAEQAYCEFAPI
DNDSDNSSEWSFEAGDEAAFTTRQIESAWSEENTEAIWSAPAASWALSDCPIEIDEND
PSPESHGDITLVESSLEASRKYLAESEEITPVNVAETPCAIADIPSSSSLIDSLVAEI
IAVATLYKDLPQLIVSGGLQAEPDSELPNTAPLAPSKSIAQTLSGDLVIQVEEVDKSK
TPLLLKRAKAKLTSFAGHAAPILKSTLMFNGVQTAAYATPLSSQEALTMENKNYRLDG
GAIIKAYSPIVYQDIRTLCGIDYHEFLDSFVLQSDLTKTKSPGKSGSDFLFTPNGKYI
IKTIKRKEHKVIANADFLADYYNHIKAHPSTQLPFYLGNYTLVADGKKTHFIIMKNLL
QKETDLIYDLKGSSHDRRAGPRKDNRGRVVFKDLDWTDKHEAISMSQKDRDKLLVQVS
KDVDFLKRHNIMDYSLLVGFQSDTRTCEQQPVIGMIDTLCPFSWRKRAETVSKSLFFG
SSAIDVVHPEKYGARFLNFVQSAVVPEPSRSVSTRY"
gene complement(<655603..>658500)
/gene="POL90"
/locus_tag="AWJ20_3907"
/db_xref="GeneID:30035980"
mRNA complement(<655603..>658500)
/gene="POL90"
/locus_tag="AWJ20_3907"
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 1"
/transcript_id="XM_018880947.1"
/db_xref="GeneID:30035980"
CDS complement(655603..658500)
/gene="POL90"
/locus_tag="AWJ20_3907"
/note="polyprotein of non-LTR retrotransposon Zorro 1 and
repeat region-related DNA"
/codon_start=1
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 1"
/protein_id="XP_018733587.1"
/db_xref="GeneID:30035980"
/translation="MSEDIHPVEYYKTLLPQIPELIIIAGDFNLHHPMWQSTEQVTRE
ANSWADWVNDNGLLLATVPNVPTHIFGNTIDLTFATPNITITPEKSEDVGSDHYLQKW
RIDTNPLDIELLQTYGGRYNYKRADWEKFEKVLDSRAALITIPKKLRGYSNRKSTHKV
ADRYATQLIGLLCDCLDEAVPKLKIVPRSKRWWTPELRQAKRDAVRARRRARSQPTEE
NRRLRQQANEEYASKILEAKTNTWNSYLASCRGTDIYDALKMIKPREPVPIMPALRLE
TGELAQTFEEQEEELYRGLFPQVKPSTRRLLKVVKTGQWKSLRMQEIEDAIRDQAPNK
APGPDGVKTVAIKRAWTVIRFRKLLKGLLQYCVRVGYHPKCFREGITVVIPKRKRDPA
LARSYRPITLLSTLGKVLEKIMQRRLTALTSGMLPVDQYGGRHGYSAVEAAHKLVHHA
EKRMKRDQVTSVLAIDIKGAFDHVHRDTLLNTMGGMGLPTAVQNWVYQFMRGRRTSLV
IDGKRTKERRIATGIPQGSPISPLLFLIYTSPLYPLIKSMGGKVIGFIDDITIYVSST
RYAENTAKLSNILARCHEWATSAHAFIDYGEKLGFMHIAKRVTPKGCRRLILPTGERI
QATSSLKLLGVTIDRQLKFEEHAKSVINKGQSALNVIRRLGGVTRGVTGATMRCLYKS
CVRSILEYASPIWYNRIRKGLKDAIQRIQNAALRSILGAYKPTAIVSLHRDAEISPIE
HRMLETEQYYLVRLHRDLHRYNPHRIRARKLYSGTMLGDRLDELYRTVEVTDIKKDRY
RLWKPPWASRDSEAISKASSQKKSIRKEIRSQCYQKWEIEYTQSPKGAFYRSFTAPRL
YSKKHANALRPFLYESPRGELSKLVQLRTGMGAMGSFFQRFRISNRRYDCRCGVEETV
EHILRDCPLTEQHRPILRDASRELDLPSLLDTRKGLKAVASFLKANPSLLS"
gene <661076..>661161
/locus_tag="AWJ20_3908"
/db_xref="GeneID:30035981"
tRNA <661076..>661161
/locus_tag="AWJ20_3908"
/product="tRNA-Pro"
/db_xref="GeneID:30035981"
gene <664264..>665214
/locus_tag="AWJ20_3909"
/db_xref="GeneID:30035982"
mRNA <664264..>665214
/locus_tag="AWJ20_3909"
/product="hypothetical protein"
/transcript_id="XM_018880948.1"
/db_xref="GeneID:30035982"
CDS 664264..665214
/locus_tag="AWJ20_3909"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733588.1"
/db_xref="GeneID:30035982"
/translation="MRSTSYTLALLSLGLASAAPVPQVPGGGAAAGLGGLVAGLGPVT
NDLSNTVGDLKPAVASLEPAVEDLGADLGDASSSLSTRQVPGGGAAAGLGGLVAGLGP
VTNDLSNTVGDLKPAVASLQPAVEDLGADLGDASSSLSTRQVPGGGAAAGLGGLVAGL
GPVTNDLSNTVGDLKPAVASLQPAVEDLGADLGDASSSLSTRQVPGGGAAAGLGGLVA
GLGPVTNDLSNTVGDLKPAVASLEPAVEDLGADLGDASSSLSTRQVPGGGAAAGLGGL
VAGLGPVTNDLSNTVEDLKPAVASLEPAVEDLGADLGASS"
gene <665497..>668793
/gene="NUP85"
/locus_tag="AWJ20_3910"
/db_xref="GeneID:30035984"
mRNA <665497..>668793
/gene="NUP85"
/locus_tag="AWJ20_3910"
/product="Nup85p"
/transcript_id="XM_018880950.1"
/db_xref="GeneID:30035984"
CDS 665497..668793
/gene="NUP85"
/locus_tag="AWJ20_3910"
/inference="similar to AA sequence:KEGG_Orthology:K14304"
/note="Subunit of the Nup84p subcomplex of the nuclear
pore complex (NPC); contributes to nucleocytoplasmic
transport and NPC biogenesis and is involved in
establishment of a normal nucleocytoplasmic concentration
gradient of the GTPase Gsp1p; also plays roles in several
processes that may require localization of genes or
chromosomes at the nuclear periphery, including
double-strand break repair, transcription and chromatin
silencing; homologous to human NUP85 aka NUP75;
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0031965 -
nuclear membrane [Evidence IEA]; GO_component: GO:0005643
- nuclear pore [Evidence IEA,IEA]; GO_component:
GO:0005643 - nuclear pore [Evidence IDA] [PMID 10684247];
GO_component: GO:0031080 - nuclear pore outer ring
[Evidence IDA] [PMID 11823431]; GO_component: GO:0031080 -
nuclear pore outer ring [Evidence IDA] [PMID 18046406];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_function: GO:0017056 - structural constituent of
nuclear pore [Evidence IMP] [PMID 22331846]; GO_process:
GO:0006406 - mRNA export from nucleus [Evidence IMP] [PMID
8565072]; GO_process: GO:0006406 - mRNA export from
nucleus [Evidence IMP] [PMID 8816998]; GO_process:
GO:0051028 - mRNA transport [Evidence IEA]; GO_process:
GO:0031081 - nuclear pore distribution [Evidence IMP]
[PMID 8565072]; GO_process: GO:0031081 - nuclear pore
distribution [Evidence IMP] [PMID 8816998]; GO_process:
GO:0045893 - positive regulation of transcription,
DNA-templated [Evidence IDA] [PMID 15817685]; GO_process:
GO:0006606 - protein import into nucleus [Evidence IMP]
[PMID 12730220]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0000055 -
ribosomal large subunit export from nucleus [Evidence IMP]
[PMID 11071906]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Nup85p"
/protein_id="XP_018733589.1"
/db_xref="GeneID:30035984"
/translation="MDYNRLFQNKDRDAGDQESSSEPKPFSFNLGGDSTTGNALQNKP
SGVGFNGLFSKQPENSYIDAQPLETNKLPDIGMVEHSIFGASATGAPPSFGAQSTETK
SLFSNSSSSGSSQPPLFGGFGRSNNDQVSREQSSVVTNSSTQGNSIFGISTNNNNSSS
SQTPSLFNKNTGPTFTSTSLFGSGSHEASVGSSGFNIQKPLSFSATSSATSESTQAPK
SEKTEDLSSTLAGKNEGFKFGSGEKSGNSWSFSSTKANETTAGQKNDSRIGTGNTNIF
NSISTGTNDSIFGQNSTAITNTGSVALPAFNLKGSASQSASDNISSQTARPSLFASSN
SNEESRFGATDSGAPLAVPSIGLDLLSQVTSLDTNYPGRDTIESIDSGFLDYEPNMDS
NNLETSQPSVSEFSTAPEFTLEDAPIQNGGLEHWHNMNRTLKFTISTVGNGGVAWIDR
RNRDKQSIQNEPTGDIIQEEKKLFSANIPSQIPNSPGYIQFVKDCYNIVKECQDEIES
DSSGPVNVVCKHYFNLLYNRILESLNYVEDDTQMMDVSFIVYCFLVVYFEANVKSESE
PFAKAFSKWANTVDEGSSPEDAAEIMRMSPPTKHPLYWDFVHDRASRGLLEVCASTLQ
EANSKEMAADVRESIHYATQLLRSYPNEEGSEYKFHQWKRQCNIAHGHAQDIADPALR
IQLVRLFDILRGHKATILEQNSNSWFVALSALVMFNDPSRSRLKEYFDIVQAEIPIDS
LLKWESGCAAVISGDILVAIQKLESLDSCVATAVAQLCQMRGLLDDYGIVRINNEPSV
RDWLVLSHGQNCITSEIPELEKIGINILVDLDIPQSRATLAEYLPRYVAHDVEELEWV
LSTANELGLHDTERQIHRIAARNYAFNGCFLEAFFELDKAKDASALQALSWQLFEDSL
VFNEPAAEDLTIQAVLPNSGVELSPLVHEIMAPFAVLNEAFELLRSEKYIQAGGRILA
LLRFPYLPGRFVAPLLSMIISLLSHNKQPRSFTSTDLVLVMDTLDMWEKQATNTKDHV
DTSGYKEGLAIIERAVHTAKTEETLPLKNNDWRALLLSIPVEKIPDFIISTGRIVLAR
EVSRAYLSGL"
gene <670651..>672309
/locus_tag="AWJ20_3911"
/db_xref="GeneID:30035985"
mRNA <670651..>672309
/locus_tag="AWJ20_3911"
/product="hypothetical protein"
/transcript_id="XM_018880951.1"
/db_xref="GeneID:30035985"
CDS 670651..672309
/locus_tag="AWJ20_3911"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733590.1"
/db_xref="GeneID:30035985"
/translation="MKKLWDSLKSNARYQAIVIGSGLVGLLYVILTSGLSLMSIKALV
IALSYCYALIMALWLMGHGLVNVPRELWLRADVNVRLNNDYRHARSVCDAFADAQSSY
VDVAAEITSIARFKDGSQHLEWIDELVEEVETTAFTIHSSAIAGHPTVNVDRSLLNEQ
YLSTLAARYYKYRARMIRHRASWQKLLKDCSIAEDIVNASRDTTNKELIFRYGRTMLP
AKLARFYYVNINMYFVRTFAVLLAILSVAIIWSELVHGTVASLVNLVISVTYGVGQQL
VSSIILGYMCIASFASLARIRVFNIYGLVHKGSDLSSLLFYASYACRLTVPLSYNYLT
LIGSRESVFEEFLGKSINLTPLGKAFNDWLPRLILLPIIFTLFHVYDKVKDYFSFELG
FDDDDEIGALNSSVIEGKELVNRALSDPNFRFAIDGAAPDSLSGANRGSPALNSRSSN
EFGGIRASRDISRSSLLPTHNLSSSPSSRTFGGATSPRPWSSPQVNEFEEPTRLTESM
NNVRSFFGNISQRVQSGISNIRQGDSVPRWARLSQSEDQDSQIL"
gene complement(<672479..>673576)
/locus_tag="AWJ20_3912"
/db_xref="GeneID:30035986"
mRNA complement(<672479..>673576)
/locus_tag="AWJ20_3912"
/product="hypothetical protein"
/transcript_id="XM_018880952.1"
/db_xref="GeneID:30035986"
CDS complement(672479..673576)
/locus_tag="AWJ20_3912"
/inference="similar to AA sequence:KEGG_Orthology:K13118"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733591.1"
/db_xref="GeneID:30035986"
/translation="MNNGSERRLVKVERDGASSESHPASDSPAGELIEFKKWPQVESS
EGGKMRLVVQQAESESITTNQTDGYRLKRPEKVIAEDEYSESLSNIISRDYFGGMPPD
TSVTPSVSLDEYQAKYTSEDNDSFNSLLEAQIQEQREKFSWLWKSNKLDSGHVESTKR
IMDKQQARFITDRAFTESERATENLGLTDRRPTRLRSNVAKDAKNALMFAPEVIGEKN
NKRPAHEVKPKKISRENTRLDISHNAKSSHNLPAVHSAVDVITPPTVNSYGFVTENTY
SYEPPAQSRRDNIHDRILKNEAEKKRQKKEESIFSFNSRSGRITKTPVSSRGASGSQV
SGTPLSPAAARLMGRVKSTKMSGFDFTPKRK"
gene <673943..>675442
/gene="ESA1"
/locus_tag="AWJ20_3913"
/db_xref="GeneID:30035987"
mRNA <673943..>675442
/gene="ESA1"
/locus_tag="AWJ20_3913"
/product="NuA4 histone acetyltransferase complex catalytic
subunit ESA1"
/transcript_id="XM_018880953.1"
/db_xref="GeneID:30035987"
CDS 673943..675442
/gene="ESA1"
/locus_tag="AWJ20_3913"
/inference="similar to AA sequence:KEGG_Orthology:K11304"
/note="Catalytic subunit of the histone acetyltransferase
complex (NuA4); acetylates four conserved internal lysines
of histone H4 N-terminal tail and can acetylate histone
H2A; required for cell cycle progression and
transcriptional silencing at the rDNA locus and regulation
of autophagy; GO_component: GO:0035267 - NuA4 histone
acetyltransferase complex [Evidence IPI] [PMID 10487762];
GO_component: GO:0035267 - NuA4 histone acetyltransferase
complex [Evidence IDA] [PMID 10911987]; GO_component:
GO:0035267 - NuA4 histone acetyltransferase complex
[Evidence IPI] [PMID 15485911]; GO_component: GO:0032777 -
Piccolo NuA4 histone acetyltransferase complex [Evidence
IDA] [PMID 12782659]; GO_component: GO:0000790 - nuclear
chromatin [Evidence IDA] [PMID 10911987]; GO_component:
GO:0005724 - nuclear telomeric heterochromatin [Evidence
IDA] [PMID 10911987]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_function: GO:0010485 - H4 histone
acetyltransferase activity [Evidence IDA] [PMID 12110674];
GO_function: GO:0004402 - histone acetyltransferase
activity [Evidence IEA]; GO_function: GO:0004402 - histone
acetyltransferase activity [Evidence IMP] [PMID 10487762];
GO_function: GO:0004402 - histone acetyltransferase
activity [Evidence IDA] [PMID 17274630]; GO_function:
GO:0034212 - peptide N-acetyltransferase activity
[Evidence IMP] [PMID 23050233]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_function:
GO:0016747 - transferase activity, transferring acyl
groups other than amino-acyl groups [Evidence IEA];
GO_process: GO:0006281 - DNA repair [Evidence IMP] [PMID
12353039]; GO_process: GO:0006281 - DNA repair [Evidence
IDA] [PMID 16135807]; GO_process: GO:0006354 -
DNA-templated transcription, elongation [Evidence IDA,IMP]
[PMID 15949446]; GO_process: GO:0016568 - chromatin
modification [Evidence IEA]; GO_process: GO:0000183 -
chromatin silencing at rDNA [Evidence IGI,IMP] [PMID
16436512]; GO_process: GO:0016573 - histone acetylation
[Evidence IDA] [PMID 10082517]; GO_process: GO:0016573 -
histone acetylation [Evidence IDA] [PMID 10487762];
GO_process: GO:0018394 - peptidyl-lysine acetylation
[Evidence IMP] [PMID 23050233]; GO_process: GO:0016239 -
positive regulation of macroautophagy [Evidence IMP] [PMID
22539722]; GO_process: GO:0032968 - positive regulation of
transcription elongation from RNA polymerase II promoter
[Evidence IGI,IMP] [PMID 19822662]; GO_process: GO:0051726
- regulation of cell cycle [Evidence IMP] [PMID 10082517];
GO_process: GO:0034401 - regulation of transcription by
chromatin organization [Evidence IMP] [PMID 11867538];
GO_process: GO:0006357 - regulation of transcription from
RNA polymerase II promoter [Evidence IMP] [PMID 11036083];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA,IEA]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="NuA4 histone acetyltransferase complex catalytic
subunit ESA1"
/protein_id="XP_018733592.1"
/db_xref="GeneID:30035987"
/translation="MTDGTPSRTASVSPGPGSGTNGEGTPAPTLPAPTITITSTQQVR
VGFKCYVEKNGEKRLAEILSTHVRKGNLEFYVHYEDFNKRLDEWVSESRIDLSQPVTL
PRPEKPKKSGSETPKAGPHNKVGKKSTKKTTKAAAAAAAAAARHSGVSTPKNKKIGAG
KENEEPVSTPGNADVMDLDDIKVNTQQDPDDFNREEEIEKLRTSGSMTQNAHEVARVR
NVSKIIMGENEIEPWYFSPYPIELTEEDQIYICEFSLSYFGSKKQFERFRSKSTLRHP
PGNEIYRDENVSFFEIDGRRQRTWCRNLCLLSKLFLDHKTLYYDVDPFLFYCMTKRDE
MGHHLVGYFSKEKESAEGYNVACILTLPQYQRHGYGRVLIDFSYNLSKKEGKVGSPEK
PLSDLGLLSYRAYWADSIVELLIESGKNDTTIEEISSSTAMTTTDIIHTLQTLGMLKY
YKGQHIICLTEAVVEKYDRQKARKRIRIDPSKLLWKPPVFTAAQLRFAW"
gene complement(<675743..>676891)
/gene="RRD2"
/locus_tag="AWJ20_3914"
/db_xref="GeneID:30035988"
mRNA complement(<675743..>676891)
/gene="RRD2"
/locus_tag="AWJ20_3914"
/product="peptidylprolyl isomerase RRD2"
/transcript_id="XM_018880954.1"
/db_xref="GeneID:30035988"
CDS complement(675743..676891)
/gene="RRD2"
/locus_tag="AWJ20_3914"
/inference="similar to AA sequence:KEGG_Orthology:K17605"
/note="Peptidyl-prolyl cis/trans-isomerase; also activates
the phosphotyrosyl phosphatase activity of protein
phosphatase 2A (PP2A); regulates G1 phase progression, the
osmoresponse, microtubule dynamics; subunit of the
Tap42p-Pph21p-Rrd2p complex; protein abundance increases
in response to DNA replication stress; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0000159 - protein phosphatase type 2A complex [Evidence
IDA] [PMID 17550305]; GO_function: GO:0016853 - isomerase
activity [Evidence IEA]; GO_function: GO:0003755 -
peptidyl-prolyl cis-trans isomerase activity [Evidence
IEA,IEA]; GO_function: GO:0003755 - peptidyl-prolyl
cis-trans isomerase activity [Evidence IDA] [PMID
16380387]; GO_function: GO:0019211 - phosphatase activator
activity [Evidence IEA]; GO_function: GO:0008601 - protein
phosphatase type 2A regulator activity [Evidence IGI,ISS]
[PMID 10660069]; GO_function: GO:0008601 - protein
phosphatase type 2A regulator activity [Evidence IMP]
[PMID 12952889]; GO_process: GO:0030472 - mitotic spindle
organization in nucleus [Evidence IMP] [PMID 11262194];
GO_process: GO:0043085 - positive regulation of catalytic
activity [Evidence IEA]; GO_process: GO:0006457 - protein
folding [Evidence IEA]; GO_process: GO:0000413 - protein
peptidyl-prolyl isomerization [Evidence IEA,IEA];
GO_process: GO:0006970 - response to osmotic stress
[Evidence IGI,IMP] [PMID 10660069]"
/codon_start=1
/product="peptidylprolyl isomerase RRD2"
/protein_id="XP_018733593.1"
/db_xref="GeneID:30035988"
/translation="MTDEKLETTEVVGSEPTNGFRVPVKRILSEKDMDLFKNSITKTE
LLGFVKDLNHAVTGITNDAEISGVAPERAVSRIVEILEKVTELIDAHPAADQTSRFGK
PEFRDLYDDIQRHSSQWIKEIVETDEKYCDGKDGNDSLVQELATYFNESWGNRQRIDY
GSGHELNFLCFLYCLKSLQLIVESEYTAVVVKIFVLYLRIMRRIQNTYWLEPAGSHGV
WGLDDYHFLPFLFGSSQLSTHKHLRPMSIHDPDIIEMYHKKYMYLGCIKFINDVKTAS
LRWHSPLIDDISGVKKWSKVNEGMLKMYNAEVLGKLPIMQHFMFGSLLPAPAGVSPES
EHSASEDDHGHVHIPNTWADCCGIKVPSAIAASEMDISGGLRRAIPFD"
gene <677182..>677685
/gene="ALG13"
/locus_tag="AWJ20_3915"
/db_xref="GeneID:30035989"
mRNA <677182..>677685
/gene="ALG13"
/locus_tag="AWJ20_3915"
/product="N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase catalytic subunit ALG13"
/transcript_id="XM_018880955.1"
/db_xref="GeneID:30035989"
CDS 677182..677685
/gene="ALG13"
/locus_tag="AWJ20_3915"
/inference="similar to AA sequence:KEGG_Orthology:K07432"
/note="Catalytic component of UDP-GlcNAc transferase;
required for the second step of dolichyl-linked
oligosaccharide synthesis; anchored to the ER membrane via
interaction with Alg14p; similar to bacterial and human
glycosyltransferases; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0043541 - UDP-N-acetylglucosamine transferase complex
[Evidence IPI] [PMID 16100110]; GO_component: GO:0043541 -
UDP-N-acetylglucosamine transferase complex [Evidence IPI]
[PMID 16100113]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14690591]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 16100110]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 16100113];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA,IEA]; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IDA] [PMID 14562095]; GO_component: GO:0042406 -
extrinsic component of endoplasmic reticulum membrane
[Evidence IDA] [PMID 16100110]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14690591]; GO_function:
GO:0004577 - N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase activity [Evidence IEA];
GO_function: GO:0004577 -
N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase activity [Evidence
IMP,ISS] [PMID 15615718]; GO_function: GO:0004577 -
N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase activity [Evidence
IDA,IMP,ISS] [PMID 16100113]; GO_function: GO:0030246 -
carbohydrate binding [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_function: GO:0016757 - transferase activity,
transferring glycosyl groups [Evidence IEA]; GO_function:
GO:0016758 - transferase activity, transferring hexosyl
groups [Evidence IEA]; GO_process: GO:0005975 -
carbohydrate metabolic process [Evidence IEA]; GO_process:
GO:0006488 - dolichol-linked oligosaccharide biosynthetic
process [Evidence IMP,ISS] [PMID 15615718]; GO_process:
GO:0006488 - dolichol-linked oligosaccharide biosynthetic
process [Evidence IDA,IMP,ISS] [PMID 16100113];
GO_process: GO:0030259 - lipid glycosylation [Evidence
IEA]"
/codon_start=1
/product="N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase catalytic subunit ALG13"
/protein_id="XP_018733594.1"
/db_xref="GeneID:30035989"
/translation="MSVLVTSGATVPFEKLIRSTLSDGVLHQLAKQGYRQITVQYGSG
KAIFDESVSSCHDPKLSIDGFDFTTDMAREIAKYDLVISHAGTGSILDALRANKKLIA
VINTSLMDNHQAEIAHEFESQGYLVSTDTESISAALQRLDSTTLKQLPPADSLKLAAI
INEEAGL"
gene complement(<677907..>678563)
/gene="ISA2"
/locus_tag="AWJ20_3916"
/db_xref="GeneID:30035990"
mRNA complement(<677907..>678563)
/gene="ISA2"
/locus_tag="AWJ20_3916"
/product="Isa2p"
/transcript_id="XM_018880956.1"
/db_xref="GeneID:30035990"
CDS complement(677907..678563)
/gene="ISA2"
/locus_tag="AWJ20_3916"
/note="Protein required for maturation of mitochondrial
[4Fe-4S] proteins; functions in a complex with Isa1p and
possibly Iba57p; localizes to the mitochondrial
intermembrane space, overexpression of ISA2 suppresses
grx5 mutations; GO_component: GO:0005758 - mitochondrial
intermembrane space [Evidence IDA] [PMID 10805735];
GO_component: GO:0005759 - mitochondrial matrix [Evidence
IEA]; GO_component: GO:0005739 - mitochondrion [Evidence
IEA]; GO_component: GO:0005739 - mitochondrion [Evidence
IDA] [PMID 16823961]; GO_function: GO:0005506 - iron ion
binding [Evidence IDA] [PMID 21987576]; GO_process:
GO:0009102 - biotin biosynthetic process [Evidence
IGI,IMP] [PMID 17259550]; GO_process: GO:0016226 -
iron-sulfur cluster assembly [Evidence IGI,IMP] [PMID
10805735]"
/codon_start=1
/product="Isa2p"
/protein_id="XP_018733595.1"
/db_xref="GeneID:30035990"
/translation="MASLYRPLVQTHSGLLAGALRLTAARATPISKRSVHSLIGSGLQ
RTQPLLTYNASAVASRSVRRIVSSPTLGPKSATKITNPIKSENGQILRVDVSQKAAEK
LNLLKSQDNNPDLALRISVESGGCHGFQYIYSLKTTKDIDSSNDSVFERDGAKVIIDA
TSLEIIQQSTIDYTTELIGSQFKVINSPFATSSCGCGSSFAFDPAAAAAAAAAATSSK
"
gene <679192..>680208
/locus_tag="AWJ20_3917"
/db_xref="GeneID:30035991"
mRNA <679192..>680208
/locus_tag="AWJ20_3917"
/product="hypothetical protein"
/transcript_id="XM_018880957.1"
/db_xref="GeneID:30035991"
CDS 679192..680208
/locus_tag="AWJ20_3917"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733596.1"
/db_xref="GeneID:30035991"
/translation="MGVIILDGTQLEEPVSAYHIPGDKGGPLFILIPGNPGVCQYYSE
YLDELKGLLPSYEFLCASHRGMDALLNPKGSVGKTTPYKLTDQVEHLCNVLEWATARA
TSSDPRDVIIMGHSMGAWLLQRVLVRFANSPKIRFKMAGLLTPTIRDLAGSPKGVKLS
WILDTVTQYPGYVMARTVQALYKTLPNFAFGYAIKLGLGGWSSTPSHAIVSTIAMLSR
PSILVQVGQLAADEISHIKSDLPEFWDASIATDRGFAIWAYFAQNDPWVSDTTRDEII
SEHSHLPSVHFDIAKGDPEDAIAHSFCLRQSSRFARITAYRISITPGLEPTKPQPPTI
SSQL"
gene complement(<682735..>684348)
/gene="PHO8"
/locus_tag="AWJ20_3918"
/db_xref="GeneID:30035992"
mRNA complement(<682735..>684348)
/gene="PHO8"
/locus_tag="AWJ20_3918"
/product="alkaline phosphatase PHO8"
/transcript_id="XM_018880958.1"
/db_xref="GeneID:30035992"
CDS complement(682735..684348)
/gene="PHO8"
/locus_tag="AWJ20_3918"
/inference="similar to AA sequence:KEGG_Orthology:K01077"
/codon_start=1
/product="alkaline phosphatase PHO8"
/protein_id="XP_018733597.1"
/db_xref="GeneID:30035992"
/translation="MADETLLPAWRRGGRSQEHNKTMVFLGIWATLATFLALYYSPGH
FSDLFKSHQSPKRNVIFFVSDGMGPASLSLTRSFRQYRDNLPIDDILHLDDHFIGHSR
TRSSDSLVTDSAAGATAFACGFKTYNGAIGVFPDTDPCGTVLEGAKLRGYHTGMVVTT
RITDATPASFSAHVLHREEEDLIAEHQLGMYPLGRMVDLMIGGGRCHFLPGSVDGGCR
HDSRNLVEEAVEDGWQYVENREQYDLLKGGNNISLPLLALLAPGDIPYDLDRDDSVYP
SLEDTTRTAIRALELATKHSDKGFFLLVEGSRIDHAGHQNDPAAQVREVLAFDRAFQA
AIEFSKKSNVETIVVSTSDHETGGLATARQLTPSYPDYLWYPEALDNATHSAEYLGHA
LRHYNGKDVYDFIKTDILKEGLGIPEPTEEQIKLLADNKKRSEDHIANIISLRSQTGW
STHGHSAVDVNVYGFSGHKRANRRILKTLGGSNENTDIGKFLADYLDVDVSKVTDLLQ
QDDITTDDLDDPHGLDSYHAHIMAVTNGL"
gene complement(<685842..>687503)
/gene="ARO8"
/locus_tag="AWJ20_3919"
/db_xref="GeneID:30035993"
mRNA complement(<685842..>687503)
/gene="ARO8"
/locus_tag="AWJ20_3919"
/product="bifunctional 2-aminoadipate
transaminase/aromatic-amino-acid:2-oxoglutarate
transaminase"
/transcript_id="XM_018880959.1"
/db_xref="GeneID:30035993"
CDS complement(685842..687503)
/gene="ARO8"
/locus_tag="AWJ20_3919"
/inference="similar to AA sequence:KEGG_Orthology:K00838"
/note="Aromatic aminotransferase I; expression is
regulated by general control of amino acid biosynthesis;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0047536 - 2-aminoadipate
transaminase activity [Evidence IEA]; GO_function:
GO:0047536 - 2-aminoadipate transaminase activity
[Evidence IDA] [PMID 19342587]; GO_function: GO:0080130 -
L-phenylalanine:2-oxoglutarate aminotransferase activity
[Evidence IEA]; GO_function: GO:0008793 -
aromatic-amino-acid:2-oxoglutarate aminotransferase
activity [Evidence IEA]; GO_function: GO:0008793 -
aromatic-amino-acid:2-oxoglutarate aminotransferase
activity [Evidence IDA] [PMID 9491082]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0030170 - pyridoxal phosphate binding
[Evidence IEA]; GO_function: GO:0008483 - transaminase
activity [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0019509 - L-methionine salvage from methylthioadenosine
[Evidence IEA]; GO_process: GO:0009094 - L-phenylalanine
biosynthetic process [Evidence IEA]; GO_process:
GO:0009072 - aromatic amino acid family metabolic process
[Evidence IGI,IMP] [PMID 9491083]; GO_process: GO:0009058
- biosynthetic process [Evidence IEA]; GO_process:
GO:0019878 - lysine biosynthetic process via aminoadipic
acid [Evidence IEA]; GO_process: GO:0006571 - tyrosine
biosynthetic process [Evidence IEA]"
/codon_start=1
/product="bifunctional 2-aminoadipate
transaminase/aromatic-amino-acid:2-oxoglutarate
transaminase"
/protein_id="XP_018733598.1"
/db_xref="GeneID:30035993"
/translation="MVTNNEIGLPHYSYFPYDTLEAAVAQPHRFSGSDKGRAQSSHIV
VPKVDSSGVNASPLDIATVLQYGTAQGIPVLYEWLKEFTTKNLHPNIPYDGGADIILS
CGNTDGMHKTLATLANDWSPARNDVSEREGLLVEKFCYMSAVDSAKFKGLNIVPVDID
YQGLLADSLEFVLANWDYSIGKRPHLLYTVSTGQNPTGGVVSVERRKEIYKVCSKYDV
VIIEDEPYWFLQYPAADEEDPNPPSTDPGSTDGHAGTKKSSGYEFLDSLVQSYLEIDT
DGRVVRLDTFSKNVAPGCRLGWITAQPAVIERITRMTEGSTQQPSGFVQSMIAKLVGA
GAPNSSAVSETGPGSFVSTLARSKREPVPEAASWSFDGWVQWLSGLRDQYQRRMVVMC
QALDDAKISVDEMSDEQGWTSVSNLENVYYDFDWPRAGMFVWLKVHLDKHPLASNSYS
NEELSTALWVWMTRAPNLVLVSPGRMFSPAESLIEDASLYFRLCFAAIAEDELKASSQ
RIASAIHSFWSVDEDTVEELIDSTRDIESNDLNFDPTSFKSVFGC"
gene complement(<687566..>687775)
/locus_tag="AWJ20_3920"
/db_xref="GeneID:30035995"
mRNA complement(<687566..>687775)
/locus_tag="AWJ20_3920"
/product="hypothetical protein"
/transcript_id="XM_018880961.1"
/db_xref="GeneID:30035995"
CDS complement(687566..687775)
/locus_tag="AWJ20_3920"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733599.1"
/db_xref="GeneID:30035995"
/translation="MAPPRDLSHYYSENAKNRQGSEFKKFYKYFAIKGIGNLAGGKLC
ESPVSTPKARALQEIAWTPDRTAGA"
gene complement(<688549..>689145)
/gene="ARL3"
/locus_tag="AWJ20_3921"
/db_xref="GeneID:30035996"
mRNA complement(<688549..>689145)
/gene="ARL3"
/locus_tag="AWJ20_3921"
/product="Arf family GTPase ARL3"
/transcript_id="XM_018880962.1"
/db_xref="GeneID:30035996"
CDS complement(688549..689145)
/gene="ARL3"
/locus_tag="AWJ20_3921"
/inference="similar to AA sequence:KEGG_Orthology:K07952"
/note="ARF-like small GTPase of the RAS superfamily;
required for recruitment of Arl1p, a GTPase that regulates
membrane traffic, to the Golgi apparatus; NatC-catalyzed
N-terminal acetylation regulates Golgi membrane
association mediated by interaction with membrane
receptor, Sys1p; similar to ADP-ribosylation factor and
orthologous to mammalian ARFRP1; GO_component: GO:0005794
- Golgi apparatus [Evidence IEA,IEA]; GO_component:
GO:0005794 - Golgi apparatus [Evidence IDA] [PMID
15077113]; GO_component: GO:0005794 - Golgi apparatus
[Evidence IDA] [PMID 15077114]; GO_component: GO:0005622 -
intracellular [Evidence IEA]; GO_component: GO:0042175 -
nuclear outer membrane-endoplasmic reticulum membrane
network [Evidence IDA] [PMID 9920936]; GO_function:
GO:0005525 - GTP binding [Evidence IEA,IEA]; GO_function:
GO:0003924 - GTPase activity [Evidence IDA,ISS] [PMID
9920936]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_process: GO:0043001 - Golgi to plasma
membrane protein transport [Evidence IMP] [PMID 16926193];
GO_process: GO:0006886 - intracellular protein transport
[Evidence IMP] [PMID 9920936]; GO_process: GO:0033365 -
protein localization to organelle [Evidence IMP] [PMID
12620189]; GO_process: GO:0015031 - protein transport
[Evidence IEA]; GO_process: GO:0007264 - small GTPase
mediated signal transduction [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Arf family GTPase ARL3"
/protein_id="XP_018733600.1"
/db_xref="GeneID:30035996"
/translation="MYHLARSLYANFTRKEEYAVLILGLDNAGKTTLLERLKTEYSNT
KGLAPDKIAPTVGQNVAHITKNGVMLKIWDLGGQEALRTLWESYYTEAHAVVFVVDST
DRTRIEQCRDALDKIVSSESIEGTPILMLANKQDQEDRLEVEDIKEIFNKIAEKMSAR
DSRVLPISALDGTGVEDATEWLVSRLVRNKQQRPPKLR"
gene <690026..>690946
/gene="ADE1"
/locus_tag="AWJ20_3922"
/db_xref="GeneID:30035997"
mRNA <690026..>690946
/gene="ADE1"
/locus_tag="AWJ20_3922"
/product="phosphoribosylaminoimidazolesuccinocarboxamide
synthase"
/transcript_id="XM_018880963.1"
/db_xref="GeneID:30035997"
CDS 690026..690946
/gene="ADE1"
/locus_tag="AWJ20_3922"
/inference="similar to AA sequence:KEGG_Orthology:K01923"
/note="N-succinyl-5-aminoimidazole-4-carboxamide ribotide
synthetase; required for 'de novo' purine nucleotide
biosynthesis; red pigment accumulates in mutant cells
deprived of adenine; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_function: GO:0004639 -
phosphoribosylaminoimidazolesuccinocarboxamide synthase
activity [Evidence IEA,IEA]; GO_function: GO:0004639 -
phosphoribosylaminoimidazolesuccinocarboxamide synthase
activity [Evidence IDA] [PMID 1756975]; GO_function:
GO:0004639 -
phosphoribosylaminoimidazolesuccinocarboxamide synthase
activity [Evidence IMP] [PMID 5767024]; GO_function:
GO:0004639 -
phosphoribosylaminoimidazolesuccinocarboxamide synthase
activity [Evidence IMP] [PMID 5767025]; GO_process:
GO:0006189 - 'de novo' IMP biosynthetic process [Evidence
IEA]; GO_process: GO:0006189 - 'de novo' IMP biosynthetic
process [Evidence IDA] [PMID 1756975]; GO_process:
GO:0006189 - 'de novo' IMP biosynthetic process [Evidence
IMP] [PMID 5767024]; GO_process: GO:0006189 - 'de novo'
IMP biosynthetic process [Evidence IMP] [PMID 5767025];
GO_process: GO:0006164 - purine nucleotide biosynthetic
process [Evidence IEA,IEA]; GO_process: GO:0006164 -
purine nucleotide biosynthetic process [Evidence IDA]
[PMID 1756975]; GO_process: GO:0006164 - purine nucleotide
biosynthetic process [Evidence IMP] [PMID 5767024];
GO_process: GO:0006164 - purine nucleotide biosynthetic
process [Evidence IMP] [PMID 5767025]"
/codon_start=1
/product="phosphoribosylaminoimidazolesuccinocarboxamide
synthase"
/protein_id="XP_018733601.1"
/db_xref="GeneID:30035997"
/translation="MSVVTTDLQGTLPLIARGKVRDLYQLSDDKLLFVATDRISAFDV
ILNNGIPSKGKLLTGLSEFWFELLADVTKNHLVPGVNSHNLAKALPQSVSSNDELISQ
LIGRSMVVNKYKVLPLEVIVRGYITGSAWAEYKKSGTVHGIPQKAGLQESEAFETPIY
TPSTKAEQGEHDENIHPDEAAKIVGPELAAKLEKLALKLYTTARDYALSKGIIIADTK
FEFGTDENGDIVLLDEVLTPDSSRFWRKDTYALGKSQDSYDKQFVRDWLISNNLKAVD
NVTIPDEIVEKTKAKYVEAYETLTGNKWVE"
gene <691740..>693089
/gene="PTC6"
/locus_tag="AWJ20_3923"
/db_xref="GeneID:30035998"
mRNA <691740..>693089
/gene="PTC6"
/locus_tag="AWJ20_3923"
/product="type 2C protein phosphatase PTC6"
/transcript_id="XM_018880964.1"
/db_xref="GeneID:30035998"
CDS 691740..693089
/gene="PTC6"
/locus_tag="AWJ20_3923"
/inference="similar to AA sequence:KEGG_Orthology:K19708"
/note="Mitochondrial type 2C protein phosphatase (PP2C);
has similarity to mammalian PP1Ks; involved in mitophagy;
null mutant is sensitive to rapamycin and has decreased
phosphorylation of the Pda1 subunit of pyruvate
dehydrogenase; GO_component: GO:0005758 - mitochondrial
intermembrane space [Evidence IEA]; GO_component:
GO:0005758 - mitochondrial intermembrane space [Evidence
IDA] [PMID 17166847]; GO_component: GO:0005759 -
mitochondrial matrix [Evidence IEA]; GO_component:
GO:0005759 - mitochondrial matrix [Evidence IDA] [PMID
18180296]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_function: GO:0004741 -
[pyruvate dehydrogenase (lipoamide)] phosphatase activity
[Evidence IEA]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0004721
- phosphoprotein phosphatase activity [Evidence IEA];
GO_function: GO:0004722 - protein serine/threonine
phosphatase activity [Evidence IDA] [PMID 17002782];
GO_process: GO:0016236 - macroautophagy [Evidence IGI]
[PMID 17166847]; GO_process: GO:0000422 - mitochondrion
degradation [Evidence IMP] [PMID 17166847]; GO_process:
GO:0043085 - positive regulation of catalytic activity
[Evidence IMP] [PMID 18180296]; GO_process: GO:0006470 -
protein dephosphorylation [Evidence IMP] [PMID 18180296]"
/codon_start=1
/product="type 2C protein phosphatase PTC6"
/protein_id="XP_018733602.1"
/db_xref="GeneID:30035998"
/translation="MLRRPRSYDPASIHPVPTNVPKLARSVSAYYTVESDNHSKARIN
LLKNPSYLGHYTSRVNRTYNEDRYYAGVLKLPVGSSLTSKYVRQKFTHHDRQVFNFAV
FDGHGGSECSQFLQDHLAEYVENCDLSSGTALRDLYRKNVGGYWRAWGPELDRYVSRL
TAVDDFQLRLPLSFLKADYEFSEKFERAGSTATSVYLYSDEESISANGDGTNLIGKTA
AANDSEDAVDYSRTPKAFWDQDQVTTLVVAHVGDTRCILCDHHGNVHQLTTNHHPSTS
VENTRLKRYATSFFTDSFGEERFGSFANTRAFGDFAAKAKGITAEPEIVECRIGDKRK
IKSLSSTYPRHIKTFTGDEAFLVLMSDGVTEMISDQEVVDLVINTANKAGSGRGTPQD
AAKEIVHYAAAVGGDDNATCMVIRLSGWGKWDTWYDRTAPRREEKLSSYSVRAPRRT"
gene complement(<702558..>705455)
/gene="POL90"
/locus_tag="AWJ20_3924"
/db_xref="GeneID:30035999"
mRNA complement(<702558..>705455)
/gene="POL90"
/locus_tag="AWJ20_3924"
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 1"
/transcript_id="XM_018880965.1"
/db_xref="GeneID:30035999"
CDS complement(702558..705455)
/gene="POL90"
/locus_tag="AWJ20_3924"
/note="polyprotein of non-LTR retrotransposon Zorro 1 and
repeat region-related DNA"
/codon_start=1
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 1"
/protein_id="XP_018733603.1"
/db_xref="GeneID:30035999"
/translation="MSEDIHPVEYYKTLLPQIPELIIIAGDFNLHHPMWQSTEQVTRE
ANSWADWVNDNGLLLATVPNVPTHIFGNTIDLTFATPNITITPEKSEDVGSDHYLQKW
RIDTNPLDIELLQTYGGRYNYKRADWEKFEKVLDSRAALITIPKKLRGYSNRKSTHKV
ADRYATQLIGLLCDCLDEAVPKLKIVPRSKRWWTPELRQAKRDAVRARRRARSQPTEE
NRRLRQQANEEYASKILEAKTNTWNSYLASCRGTDIYDALKMIKPREPVPIMPALRLE
TGELAQTFEEQEEELYRGLFPQVKPSTRRLLKVVKTGQWKSLRMQEIEDAIRDQAPNK
APGPDGVKTVAIKRAWAVIRFRKLLKGLLQYCVRVGYHPKCFREGITVVIPKRKRDPA
LARSYRPITLLSTLGKVLEKIMQRRLTALTSGMLPVDQYGGRHGYSAVEAAHKLVHHA
EKRMKRDQVTSVLAIDIKGAFDHVHRDTLLNTMGGMGLPTAVQNWVYQFMRGRRTSLV
IDGKRTKERRIATGIPQGSPISPLLFLIYTSPLYPLIKSMGGKVIGFIDDITIYVSST
RYAENTAKLSNILARCHEWATSAHAFIDYGEKLGFMHIAKRVTPKGCRRLILPTGERI
QATSSLKLLGVTIDRQLKFEEHAKSVINKGQSALNVIRRLGGVTRGVTGATMRCLYKS
CVRSILEYASPIWYNRIRKGLKDAIQRIQNAALRSILGAYKPTAIVSLHRDAEIPPVE
HRMLETEQYYLVRLHRDLHRYNPHRIRARKLYFGTMLGDRLDELYRTVEVTDIKKDRY
RLWKPPWASRDSEAISKASSQKKSIRKEIRSQCYQKWEIEYTQSPKGAFYRSFTAPRL
YSTKHANALRPFLYESPRGELSKLVQLRTGMGAMGSFFQRFRISNRRYDCRCGVEETV
EHILRDCPLTEQHRPILRDASRELDLPSLLDTRKGLKAVASFLKANPSLLS"
gene complement(<717614..>718165)
/locus_tag="AWJ20_3925"
/db_xref="GeneID:30036000"
mRNA complement(<717614..>718165)
/locus_tag="AWJ20_3925"
/product="hypothetical protein"
/transcript_id="XM_018880966.1"
/db_xref="GeneID:30036000"
CDS complement(717614..718165)
/locus_tag="AWJ20_3925"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733604.1"
/db_xref="GeneID:30036000"
/translation="MTDNYHASNDLLPGKASDVNDQSNNRHEEQFQDTAAKATNGIKH
EPQASNLEPLPPKKIRRKASTLPPPVARKDKGAALSLEEKKANHIASEQRRRQAIRQE
FDKICELVPDLDLSKSRSEVVVLERTVSFLLHLMEENKALRELATSHSIELPADLAAD
TDNRRIIPANSASGYETSTHSRS"
gene <718557..>718674
/locus_tag="AWJ20_3926"
/db_xref="GeneID:30036001"
tRNA <718557..>718674
/locus_tag="AWJ20_3926"
/product="tRNA-Pro"
/db_xref="GeneID:30036001"
gene complement(<719248..>719544)
/locus_tag="AWJ20_3927"
/db_xref="GeneID:30036002"
mRNA complement(<719248..>719544)
/locus_tag="AWJ20_3927"
/product="hypothetical protein"
/transcript_id="XM_018880967.1"
/db_xref="GeneID:30036002"
CDS complement(719248..719544)
/locus_tag="AWJ20_3927"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733605.1"
/db_xref="GeneID:30036002"
/translation="MSSAASKRLIKELSAFSHDPNPALDALQLQSEDDLFTWLAVLRG
PADSPYEGQYENDLNALHCVFAFYLLTQRRWTLAYTDKGSTKLSSSTADDAISY"
gene <720516..>724226
/gene="AOH2"
/locus_tag="AWJ20_3928"
/db_xref="GeneID:30036003"
mRNA <720516..>724226
/gene="AOH2"
/locus_tag="AWJ20_3928"
/product="xanthine dehydrogenase/oxidase"
/transcript_id="XM_018880968.1"
/db_xref="GeneID:30036003"
CDS 720516..724226
/gene="AOH2"
/locus_tag="AWJ20_3928"
/inference="similar to AA sequence:KEGG_Orthology:K00106"
/codon_start=1
/product="xanthine dehydrogenase/oxidase"
/protein_id="XP_018733606.1"
/db_xref="GeneID:30036003"
/translation="MSLYALLRNCYNRHPTQQEISEAFDGNLCRCTGYKPILDAANSF
VEGGGIVSKENGNAETNGTSTGSGGCCRMSNDQACACMRNQKSPNGFPLKEHNFHEQA
RGQSGSPVELIFPPGLKKFEPVPLFYGNERKVWFRPLTKRQLLEIKDRYPKAKLVGGA
TEIQIEIKMKQMDYNVSVFANDIDELKQFHYDEERKGVEFGANIDLSTLEHRLNEMTS
LIGTEKSQIYQEIVKQLKYFAGRQIRNVATPAGNIATASPISDLNPVLVAADAVLTVE
SLSDGIQEIPISKFFIGYRKTLLPDGGIISKIFVPESLPQREVVRAFKQAKRKDDDIA
IVTACLRLVVDKDFKITKARLAYGGVAPMTVRALKTEERLLGMEVSDSTSDAIISLSV
NSLNNDDFKNITFGVPGGMAIFRRTLVTSFFYKFYQTVLQSFGFIRTLDGDAVEEVTR
NFPIGKRDLDNAFEKKVLGKSNPHMSALKQVTGEATYVDDIPPFHKELFGVQVMSTRA
HARIVSVDYSPIFEIDGVVGYANVDDIVSKQANMWGHFEDGKEEFFADGNVRYIGQCI
GLVVAKDRETAARGARAVVVEYEDIDPKVITIEDAIKYNSFFNLERKIVKGDVESAFQ
TAAHIVEGTTRIGAQEHFYLETHGCIAVPEEDNEMKIYTSGQAPNATQVSAAQVLGIP
ANRIVARVKRLGGGFGGKESRCCQISSIAAVAARKFKKPVRIILTRSEDMLTMGQRHP
FLIRWKVGLDKNFRFIGLKSKLYANAGWSLDLTAGVVERALFHMDNCYDFQNCHFEGF
ACKTNTASNTAFRGFGGPQGMFGAESLIYEVAETLGIEPDTLRELNYFRPGSSTAYQQ
PMNEDFTVPLLAKQVKEQANFQQLRAEVDAFNKEHKWIKRGLAHVPTAFGVSFGALFL
NQAGALIHIYEDGSVLLAHGGTEMGQGLFTKMAMVAAEELDVPLESVFTSESATNTVA
NASPTAASASSDLNGMAVKDACDQLNKRLKPYREKFGPEATMKELAHAAYFDRVNLSA
NGFYKTPDIGYVWGDPNPKPAFFYHTQGAAVSMVEVNTLTGDWSCLRTDIKMDVGRPL
NQAIDYGQIEGAYIQGQGLFTMEESLWLQSGALFTRGPGAYKIPGFRDIPQIFNVGIL
QDRPFKHLKTINRSKGIGEPPLFLGSTVFFAIRDALLAARKQNGKTEPLVGVQSPLTS
ERIRNLCGDSLVDLSIQAVQPTMDQKEFFVVA"
gene complement(<725372..>726289)
/gene="SAY1"
/locus_tag="AWJ20_3929"
/db_xref="GeneID:30036004"
mRNA complement(<725372..>726289)
/gene="SAY1"
/locus_tag="AWJ20_3929"
/product="Say1p"
/transcript_id="XM_018880969.1"
/db_xref="GeneID:30036004"
CDS complement(725372..726289)
/gene="SAY1"
/locus_tag="AWJ20_3929"
/note="Sterol deacetylase; component of the sterol
acetylation/deacetylation cycle along with Atf2p; active
both in the endoplasmic reticulum (ER) and in lipid
droplets; integral membrane protein with active site in
the ER lumen; green fluorescent protein (GFP)-fusion
protein localizes to the ER; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IDA] [PMID
14562095]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IDA] [PMID 24868093]; GO_component:
GO:0005788 - endoplasmic reticulum lumen [Evidence IDA]
[PMID 18034159]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IDA] [PMID 18034159]; GO_component:
GO:0005811 - lipid particle [Evidence IDA] [PMID
24868093]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0034084 - steryl
deacetylase activity [Evidence IDA,IMP] [PMID 18034159];
GO_process: GO:0009636 - response to toxic substance
[Evidence IMP] [PMID 18034159]; GO_process: GO:0034210 -
sterol deacetylation [Evidence IMP] [PMID 18034159];
GO_process: GO:0016125 - sterol metabolic process
[Evidence IMP] [PMID 18034159]"
/codon_start=1
/product="Say1p"
/protein_id="XP_018733607.1"
/db_xref="GeneID:30036004"
/translation="MPTSAAKVLGWTMKKPFKNYGKKYASDGFTAYWLVELDDRQPED
PVLIFCHGGGFVFPLSLPQVWLLTAIVRAFPSVRLNVLILDYSLSPEHRYPTQIEEIA
SLYRHLLDVEACENIVLAGESCGGNLILALLAHLKHGFPTASPTTSKCKDTAVKGAIL
ISPWVNLAIEAVVHDADSSYVKNAHEDILNTERLCFWANEYCPDKLTRQTSPWVSPVL
LIKENIGFWENVLPARTLITWGEEELMKDDVARFASMTNISNAFEVPNGVHVDVLFQS
KSPVYDKIIAFLEGVFEAKSVDSSTSTIL"
gene <728241..>729293
/locus_tag="AWJ20_3930"
/db_xref="GeneID:30036006"
mRNA <728241..>729293
/locus_tag="AWJ20_3930"
/product="hypothetical protein"
/transcript_id="XM_018880971.1"
/db_xref="GeneID:30036006"
CDS 728241..729293
/locus_tag="AWJ20_3930"
/inference="similar to AA sequence:KEGG_Orthology:K00459"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733608.1"
/db_xref="GeneID:30036006"
/translation="MATNWLFTELKLRIPVIQAPMAGVSSSSMVVAASKAGILGSLGG
GMMQPEVLRKEIEAIRNQTDRPFNINLFVIDNYEQGFDVRTKDIQWLKDYYKQEGVEF
NLPKSFAPKFQDQFETLLELAPPVASFAFGILSAEQVDACHERGIRVIGTATSGEEAV
AWEKVGADAICAQGTEAGGHRGNFINLDDPGLGLFALIPEIKRQVKIPVIAAGGIMSG
QGIVASRLLGADVAQLGTYFLASTEAGIPSGYLKSLAGQKSGSNTSLTKGFSGRYARG
IVNKFMNTSKEHSIYPYPIQNALTQPLRKATLAKGDAESYSLWSGQGLYQVKEGESIS
DLVDSLEREISQTKLP"
gene <729621..>731246
/gene="COQ6"
/locus_tag="AWJ20_3931"
/db_xref="GeneID:30036007"
mRNA <729621..>731246
/gene="COQ6"
/locus_tag="AWJ20_3931"
/product="putative N,N-dimethylaniline monooxygenase COQ6"
/transcript_id="XM_018880972.1"
/db_xref="GeneID:30036007"
CDS 729621..731246
/gene="COQ6"
/locus_tag="AWJ20_3931"
/inference="similar to AA sequence:KEGG_Orthology:K06126"
/note="Putative flavin-dependent monooxygenase; involved
in ubiquinone (Coenzyme Q) biosynthesis; localizes to the
matrix face of the mitochondrial inner membrane in a large
complex with other ubiquinone biosynthetic enzymes; human
COX6 can rescue a yeast cox6 mutant and is implicated in
steroid-resistant nephrotic syndrome (SRNS); GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence
IEA,IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IDA] [PMID 12721307]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0004499 -
N,N-dimethylaniline monooxygenase activity [Evidence ISA]
[PMID 12721307]; GO_function: GO:0050660 - flavin adenine
dinucleotide binding [Evidence IEA]; GO_function:
GO:0004497 - monooxygenase activity [Evidence IEA,IEA];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_function: GO:0016709 -
oxidoreductase activity, acting on paired donors, with
incorporation or reduction of molecular oxygen, NAD(P)H as
one donor, and incorporation of one atom of oxygen
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0006744 - ubiquinone biosynthetic process
[Evidence IEA,IEA,IEA]; GO_process: GO:0006744 -
ubiquinone biosynthetic process [Evidence IMP] [PMID
21944752]; GO_process: GO:0006744 - ubiquinone
biosynthetic process [Evidence IMP] [PMID 9266513]"
/codon_start=1
/product="putative N,N-dimethylaniline monooxygenase COQ6"
/protein_id="XP_018733609.1"
/db_xref="GeneID:30036007"
/translation="MSGLIRLGWRPLSRARFIRKPSPAVGSHSKLLASRSQWGLIRHL
STVGPILQAEPSSIDNQSIDASKTDKAGAVNEQKVSYHDVVIIGGGPAGLTLFTALKN
SPVTSNLDIVLVEGSSLERLRSWSLPENLYENRVSSLTPRSVEFLKSIGAWDHVHHER
VQDYHEMKVWDGCSDSRIEFDPYILGENNEIAWMIENSNLQNSLLSRLAELDSSASVN
NVVDSAKVKSISRQPSEQNNKQDGQVSLGAATWDSSSWPVVELENGRKLSARLLVGAD
GGNSPVRTFAGIQSRGWDYERHGLVASVKLEWEDFRSIAWQRFLVTGPIALLPLPNGF
CSLVWSTTPENAQHLKSLTPAAFCAMVNAAFRLGPVDLKYLHTITDEATLIDEIEWRI
QNTNIVDEDNNVPVPISEVLDKSRASFPLKMKHADTYVSERVALVGDAAHTTHPLAGQ
GLNMGQSDVANLVQAIETATKRGLDIGSLLALEPYWASSYWSNHIKLGVVDKLHKLYS
SDFGPLVQLRSWGLDFFNESPFLKRLLMSNASK"
gene <731869..>733311
/locus_tag="AWJ20_3932"
/db_xref="GeneID:30036008"
mRNA <731869..>733311
/locus_tag="AWJ20_3932"
/product="hypothetical protein"
/transcript_id="XM_018880973.1"
/db_xref="GeneID:30036008"
CDS 731869..733311
/locus_tag="AWJ20_3932"
/inference="similar to AA sequence:KEGG_Orthology:K12735"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733610.1"
/db_xref="GeneID:30036008"
/translation="MVVKNIASNSDEPSLLAGSQFIVTLGDGLDSLDSRAVVFGRVEE
GYDTLDTISNAICDSDGRPLKDIRIKHTYILEDPFPDPNGLDELVRPNSPEPSEKQLS
TVRIGDDNDESNEDEENAENKHKEQDARAQALTLEMVGDLPFAEVKPMENVLFVCKLN
PVTNDEDLELIFSRFGPIISCEVIRDKDTGDSLQYAFIEFEDQEACERAYLKMDGVLI
DDNRIHVDFSQSVSRLSDGWRNETNAKRKNVHGSKFISNASTSVKSRNVDQRDIKRQR
LRDGNVNSTRYDGRESRRYGMDETHYERKVDSRYEDKKTHESYRQDERYEEDRLDKQY
HRKHDKSDNKRDERRGNRRDDRRDDKRDDRRDDQRDDRRDDRRDDRREDRRDDRRDDR
RDHRRDHRRDDGRDEKSDDRRSHRRDDTKYDRSDSTKDREDRSYRDRERYGHSRDSYR
DRRDRDQGRHARRDEGRHNRHNSDRTQSRD"
gene complement(<733458..>734279)
/locus_tag="AWJ20_3933"
/db_xref="GeneID:30036009"
mRNA complement(<733458..>734279)
/locus_tag="AWJ20_3933"
/product="hypothetical protein"
/transcript_id="XM_018880974.1"
/db_xref="GeneID:30036009"
CDS complement(733458..734279)
/locus_tag="AWJ20_3933"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733611.1"
/db_xref="GeneID:30036009"
/translation="MGSPSFIHKPFPISQITPSTIVLGSSGFKNTTASAEDTSVGTEG
SIRTTSSDITEGSQDSLSDRNTDISSLSSTESASGTHADSKTNDFLATAVQTNIAINM
ASSATIRSNSQESDHNESASENNNDGNIRSTANSTTHSAANSSPASPLPSSPTIMNTI
LGPATQKTSSTITIPFSDTQIEASSVLVTSSDPPERCTSPRRDNVAIEKIGREKVQVL
RKEAQLIQESLTDILERIIKVKEDYDRLSNENRFLQDYIGNLMSTSNILNKAGHA"
gene <736051..>737364
/gene="TGL2"
/locus_tag="AWJ20_3934"
/db_xref="GeneID:30036010"
mRNA <736051..>737364
/gene="TGL2"
/locus_tag="AWJ20_3934"
/product="Tgl2p"
/transcript_id="XM_018880975.1"
/db_xref="GeneID:30036010"
CDS 736051..737364
/gene="TGL2"
/locus_tag="AWJ20_3934"
/inference="similar to AA sequence:KEGG_Orthology:K01046"
/note="Triacylglycerol lipase that is localized to the
mitochondria; has lipolytic activity towards
triacylglycerols and diacylglycerols when expressed in E.
coli; GO_component: GO:0005739 - mitochondrion [Evidence
IDA] [PMID 19959834]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0016788 -
hydrolase activity, acting on ester bonds [Evidence IEA];
GO_function: GO:0004806 - triglyceride lipase activity
[Evidence IEA]; GO_function: GO:0004806 - triglyceride
lipase activity [Evidence IDA,IMP] [PMID 9544243];
GO_process: GO:0006505 - GPI anchor metabolic process
[Evidence IEA]; GO_process: GO:0006886 - intracellular
protein transport [Evidence IEA]; GO_process: GO:0016042 -
lipid catabolic process [Evidence IEA]; GO_process:
GO:0006629 - lipid metabolic process [Evidence IEA];
GO_process: GO:0019433 - triglyceride catabolic process
[Evidence IMP] [PMID 19959834]"
/codon_start=1
/product="Tgl2p"
/protein_id="XP_018733612.1"
/db_xref="GeneID:30036010"
/translation="MMSSTDLLLAYSAASQQRYQKETDDSGNSRARLMVVLKGVGVNG
GIAAVAGSAAKHLSFLCKVGGPYGLGLFNTTTPARFYSDNNGSGTGKGGNPNDQIDGL
KNRKEIEGDPANVRDGNLRKEESSDSTVIGKPGSSKSPPKTLLLKHEFENLKAKYTPT
KYPIILCHGLFGFDTLKSPVPIIVKDLSYWHGIKEALVDAGAKVLSASVPPVGPIETR
AEYLAQVIERWVKEENLDNGNGESDEPLKLNLIGHSMGGLDARYMISVLKPPNIKVLS
LTTIVTPHQGTSFADHIFKENPLYITKLLKAIACVGGVEEPSGIKQLTTEHMQNAFNP
HVPDDKDVYYSSYGARIGSRWTTVLKYSADYIKSVEGDNDGLVSVESAKWGEYLGTIE
NCDHLDVVNLVGVTHWLKWATFQKSSFNAIALYLHIMDSLAKKGF"
gene complement(<737539..>738930)
/gene="ALD5"
/locus_tag="AWJ20_3935"
/db_xref="GeneID:30036011"
mRNA complement(<737539..>738930)
/gene="ALD5"
/locus_tag="AWJ20_3935"
/product="aldehyde dehydrogenase (NAD(P)(+)) ALD5"
/transcript_id="XM_018880976.1"
/db_xref="GeneID:30036011"
CDS complement(737539..738930)
/gene="ALD5"
/locus_tag="AWJ20_3935"
/inference="similar to AA sequence:KEGG_Orthology:K00128"
/note="Mitochondrial aldehyde dehydrogenase; involved in
regulation or biosynthesis of electron transport chain
components and acetate formation; activated by K+;
utilizes NADP+ as the preferred coenzyme; constitutively
expressed; GO_component: GO:0005759 - mitochondrial matrix
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0004029 - aldehyde dehydrogenase (NAD) activity
[Evidence IDA] [PMID 9473035]; GO_function: GO:0004030 -
aldehyde dehydrogenase [NAD(P)+] activity [Evidence IEA];
GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
activity [Evidence IDA] [PMID 9473035]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA,IEA];
GO_function: GO:0016620 - oxidoreductase activity, acting
on the aldehyde or oxo group of donors, NAD or NADP as
acceptor [Evidence IEA]; GO_process: GO:0019413 - acetate
biosynthetic process [Evidence IMP] [PMID 15256563];
GO_process: GO:0006068 - ethanol catabolic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="aldehyde dehydrogenase (NAD(P)(+)) ALD5"
/protein_id="XP_018733613.1"
/db_xref="GeneID:30036011"
/translation="MLRGILRSQSSQWTKGFRQTVRQYSSSKSSDIADKAVESAFKSS
ATWKNVPVEEKRDIFYNASKLLKERREELVKLTNQEVGIPSLFAGFFVDDAVDFMTTM
GDLVTNSGYGQFLPPVKGRQPIVVREPIGPVFGIVPWNAPTILGLRAAACPLAAGCSV
VVKTSEYTPLTQRLTLQTLTDAGVPEGVVNILEVPLEDSPAVVEQVISDFRIRKVNFT
GSGRVGAIVAGLAGTHLKPILLELGGKAAAIVDETADFETAARNIVLGAWANSGQICM
STERIFVTKEAADGFNKAITKVSHEELNQDYLQCRQISPVNSKRIEQLAKDAVSKGAT
SIVNGIPKAKDGDVSKSILTNVSQDSELFDSETFAPLAFFNIVDSVDQAIDLVNSSKF
GLNSSVWTADTAKGIEIARKINSGTVHINGNTVFDHGSVPHGGVKNSGFGRFNGQWGI
DEFTVIKTITYPV"
gene <740233..>741807
/locus_tag="AWJ20_3936"
/db_xref="GeneID:30036012"
mRNA <740233..>741807
/locus_tag="AWJ20_3936"
/product="hypothetical protein"
/transcript_id="XM_018880977.1"
/db_xref="GeneID:30036012"
CDS 740233..741807
/locus_tag="AWJ20_3936"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733614.1"
/db_xref="GeneID:30036012"
/translation="MTLKSETSLKSVNVKLEGIAKTVIQVTEEVNDRGKKRETKTRDE
IEIHHVLYKTQTVFPPEDVRKSSTSNQFTLPPGSYTYAFRFKIPVNSDCRQNAASSAS
NSWGNGSNGWTPGGPGGSFAGGAFSSIANGRIGSSISNSINNVAGPGNKLLVTGRGID
IARAPSLQSHDGGVLPPSLSGIENSASIKYFVKVTVVRASMLKMNTRSHKPFIFLPLD
PPRLYDSSKMAFVRRQITVGTSVTSPGGSKGRSTSSSGGGGFFSSLLSSQKVSSFMGV
NTWPGETLSFTFEMRFPSVGTFVPLRPLPIELYALFPANPTTMTERKAIYISSFSVFL
YARTFIRAHEHNKEIPLQLNVCDKKNLRLAVSLADAQPVTRNGQTVFELRLPSDLLNG
VQFPSYVSPTFTTCNIARNYSIGVEMGFYCVQGIPVDHVGLLSDIIVRSGVSAPSENT
SSALPPPPPSRPVQSDPPPEKLKYHTNAEDTQENGELPSYNEVVAATVGTSSSTQPEP
RRKFEQAPDYYKISKE"
gene complement(<742105..>743130)
/locus_tag="AWJ20_3937"
/db_xref="GeneID:30036013"
mRNA complement(<742105..>743130)
/locus_tag="AWJ20_3937"
/product="hypothetical protein"
/transcript_id="XM_018880978.1"
/db_xref="GeneID:30036013"
CDS complement(742105..743130)
/locus_tag="AWJ20_3937"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733615.1"
/db_xref="GeneID:30036013"
/translation="MGSGAEPQSPEAYNNNPTLAAGEGVYLDRDSSGDKGLCMHVPLK
RISVRNNCKAGVRLAHFSKVHGLFQYSQRFNVTGSRSFSVDIFLPLIFAQYLLASGYW
SHSHLNLKDNIRRNYKMTNDTASSYSDEYKVIRNHPYVIELLDSGNYEVNHAYDVPDD
QKDHSLTAGTLIGKDLISHTPLALFLRSDLITERQESELIVFYHLGSRLCGHKGIIHG
GLLATLIDETFCRCGFPLLPNKLGVTATLDIQYLAPTKAESIIVVRAKTTKVEGRKVW
IEGAVSALPYPDAYESGHELTPAVKAKVLLVEPRWVEKLPKFSEGKTELATEEGTSEA
QQQQPTA"
gene <746553..>748787
/gene="ORC1"
/locus_tag="AWJ20_3938"
/db_xref="GeneID:30036014"
mRNA <746553..>748787
/gene="ORC1"
/locus_tag="AWJ20_3938"
/product="origin recognition complex subunit 1"
/transcript_id="XM_018880979.1"
/db_xref="GeneID:30036014"
CDS 746553..748787
/gene="ORC1"
/locus_tag="AWJ20_3938"
/inference="similar to AA sequence:KEGG_Orthology:K02603"
/note="Largest subunit of the origin recognition complex;
involved in directing DNA replication by binding to
replication origins; also involved in transcriptional
silencing; exhibits ATPase activity; ORC1 has a paralog,
SIR3, that arose from the whole genome duplication;
GO_component: GO:0031261 - DNA replication preinitiation
complex [Evidence IDA] [PMID 9554851]; GO_component:
GO:0005664 - nuclear origin of replication recognition
complex [Evidence IDA,IMP] [PMID 9372948]; GO_component:
GO:0005656 - nuclear pre-replicative complex [Evidence
IDA] [PMID 16824194]; GO_component: GO:0005656 - nuclear
pre-replicative complex [Evidence IDA] [PMID 9335335];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11168584]; GO_component: GO:0000808 - origin recognition
complex [Evidence IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA,IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IDA] [PMID 17825064]; GO_function:
GO:0005524 - ATP binding [Evidence IMP] [PMID 9038340];
GO_function: GO:0016887 - ATPase activity [Evidence IMP]
[PMID 9038340]; GO_function: GO:0003677 - DNA binding
[Evidence IEA]; GO_function: GO:0003688 - DNA replication
origin binding [Evidence IDA] [PMID 16824194];
GO_function: GO:0003682 - chromatin binding [Evidence
IEA]; GO_function: GO:0003682 - chromatin binding
[Evidence IDA] [PMID 11168584]; GO_function: GO:0017111 -
nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_process: GO:0006260 - DNA replication
[Evidence IEA,IEA]; GO_process: GO:0006270 - DNA
replication initiation [Evidence IMP] [PMID 16716188];
GO_process: GO:0030466 - chromatin silencing at silent
mating-type cassette [Evidence IDA] [PMID 12897051];
GO_process: GO:0030466 - chromatin silencing at silent
mating-type cassette [Evidence IDA,IGI] [PMID 16581798];
GO_process: GO:0006267 - pre-replicative complex assembly
involved in nuclear cell cycle DNA replication [Evidence
IDA] [PMID 16824194]; GO_process: GO:0006267 -
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [Evidence IMP] [PMID 9335335]"
/codon_start=1
/product="origin recognition complex subunit 1"
/protein_id="XP_018733616.1"
/db_xref="GeneID:30036014"
/translation="MSDEDEWKYIYPSSENDAPDDESPRKRRTGLRNTSVLSPIGVRR
LADEQEFYVGDCVLLDSGSDTPYVGIIESFFFSEKGFMETRTIWFRRFSEITRQSARK
NAPENLEGELFATPDMGRDSLDILLEPAIVLSYSEYQNRIAATSDTPIEEKNTFFCRR
TFNSDRGLFGKEDFDWSTVYAGRGSDMLQLLANLEDLTPSTRKKTATPRASPAKKQPP
KKKIIELSSSDEEDELLSGSDSDEFFDTAEDKAGIRTSPSPTPSKRKRTSASEPKTPT
KSRKKASISNTPKTPKSSTVSPVKKRSTATPRRKLNPLQPTAVHALPVRMSPTKQVPT
SPHKFARDVLHVATVPDSLPCRESEFSQIFLALESAISSESGTCVYISGTPGTGKTAT
VREVIAQLQLRVEDEELPAFKFVEINGMKLTNPHTAYEILYENISNKKAAAATAMVLL
EKQFKSSSDNNSMPVVVLMDELDQLVTKNQGVMYNFFNWPTFSHSKLIVVAVANTMDL
PERMLSNKISSRLGLTRIQFPGYSHEQLREIISTRLANLPEGAGIVEKDAIEFASRKI
AGVSGDARRALDICRRAVELAQPEEEEISDTLNDGEKGSLDEHKVTIDHIKKAIAEST
SSPTHTFLRGLSMSAKILVCAMLSRVRRSGLIDNPLGDVLQETERIVKLSNDSEKLTA
VLFNNNRVRIAGFQYALNELIEGGVLLQQMLRGERSANIRLSIGEEYVKSAFKNDLQV
AGML"
gene complement(<748898..>749689)
/gene="SEC14"
/locus_tag="AWJ20_3939"
/db_xref="GeneID:30036015"
mRNA complement(<748898..>749689)
/gene="SEC14"
/locus_tag="AWJ20_3939"
/product="phosphatidylinositol/phosphatidylcholine
transfer protein SEC14"
/transcript_id="XM_018880980.1"
/db_xref="GeneID:30036015"
CDS complement(748898..749689)
/gene="SEC14"
/locus_tag="AWJ20_3939"
/note="Phosphatidylinositol/phosphatidylcholine transfer
protein; involved in regulating PtdIns, PtdCho, and
ceramide metabolism, products of which regulate
intracellular transport and UPR; has a role in
localization of lipid raft proteins; functionally
homologous to mammalian PITPs; SEC14 has a paralog,
YKL091C, that arose from the whole genome duplication;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0005794 - Golgi apparatus [Evidence IDA]
[PMID 1997207]; GO_component: GO:0000139 - Golgi membrane
[Evidence IEA]; GO_component: GO:0000139 - Golgi membrane
[Evidence IDA] [PMID 7816798]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 1997207]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 12869188];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
2466847]; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_function: GO:0008525 -
phosphatidylcholine transporter activity [Evidence
IDA,IMP] [PMID 10488334]; GO_function: GO:0008525 -
phosphatidylcholine transporter activity [Evidence IDA]
[PMID 10848624]; GO_function: GO:0008525 -
phosphatidylcholine transporter activity [Evidence IDA]
[PMID 2407740]; GO_function: GO:0008526 -
phosphatidylinositol transporter activity [Evidence
IDA,IMP] [PMID 10848624]; GO_function: GO:0008526 -
phosphatidylinositol transporter activity [Evidence
IDA,IMP] [PMID 2215682]; GO_function: GO:0008526 -
phosphatidylinositol transporter activity [Evidence IDA]
[PMID 2407740]; GO_function: GO:0005215 - transporter
activity [Evidence IEA]; GO_process: GO:0043001 - Golgi to
plasma membrane protein transport [Evidence IMP] [PMID
10848624]; GO_process: GO:0043001 - Golgi to plasma
membrane protein transport [Evidence IMP] [PMID 19129178];
GO_process: GO:0043001 - Golgi to plasma membrane protein
transport [Evidence IMP] [PMID 6754086]; GO_process:
GO:0006896 - Golgi to vacuole transport [Evidence IGI,IMP]
[PMID 18753406]; GO_process: GO:0048194 - Golgi vesicle
budding [Evidence IDA] [PMID 9553090]; GO_process:
GO:0048194 - Golgi vesicle budding [Evidence IDA] [PMID
9736710]; GO_process: GO:0030437 - ascospore formation
[Evidence IMP] [PMID 14528019]; GO_process: GO:2001246 -
negative regulation of phosphatidylcholine biosynthetic
process [Evidence IDA,IMP] [PMID 7816798]; GO_process:
GO:1901352 - negative regulation of phosphatidylglycerol
biosynthetic process [Evidence IMP] [PMID 12869188];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IGI,IMP] [PMID 10567405]; GO_process:
GO:0015914 - phospholipid transport [Evidence IDA] [PMID
10848624]; GO_process: GO:0015914 - phospholipid transport
[Evidence IDA,IMP] [PMID 2215682]; GO_process: GO:0015914
- phospholipid transport [Evidence IDA] [PMID 2407740];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA,IEA]"
/codon_start=1
/product="phosphatidylinositol/phosphatidylcholine
transfer protein SEC14"
/protein_id="XP_018733617.1"
/db_xref="GeneID:30036015"
/translation="MYIACEKWREEFGTNTILEDFVYEEKPQVAKYYPQYYHKTDKDG
RPVYIEQLGAVKISEMYKITSQERMLKNLVWEYEAFVRYRLPATSRQRGYLVETSCTI
LDLKGCSLSTASSVYSYLREASAIGQNYYPERMGKFYLLNAPFGFATVFSMIKGFLDP
VTVEKIHILGSKYQSELLSQIPAENLPAKFGGKSVSAGSVELADDGPWRDMQFVGPEG
LAPKSSVSVLSSIAGTPSPSSAASSRPPSRAASISSKRSQKLQPQ"
gene <751932..>753830
/gene="NUP84"
/locus_tag="AWJ20_3940"
/db_xref="GeneID:30036017"
mRNA <751932..>753830
/gene="NUP84"
/locus_tag="AWJ20_3940"
/product="Nup84p"
/transcript_id="XM_018880982.1"
/db_xref="GeneID:30036017"
CDS 751932..753830
/gene="NUP84"
/locus_tag="AWJ20_3940"
/inference="similar to AA sequence:KEGG_Orthology:K14301"
/note="Subunit of the Nup84p subcomplex of the nuclear
pore complex (NPC); contributes to nucleocytoplasmic
transport and NPC biogenesis; also plays roles in several
processes that may require localization of genes or
chromosomes at the nuclear periphery, including
double-strand break repair, transcription and chromatin
silencing; homologous to human NUP107; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0031965 - nuclear
membrane [Evidence IEA]; GO_component: GO:0005643 -
nuclear pore [Evidence IEA,IEA,IEA]; GO_component:
GO:0005643 - nuclear pore [Evidence IDA] [PMID 10684247];
GO_component: GO:0031080 - nuclear pore outer ring
[Evidence IDA] [PMID 11823431]; GO_component: GO:0031080 -
nuclear pore outer ring [Evidence IDA] [PMID 18046406];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_function: GO:0017056 - structural constituent of
nuclear pore [Evidence IMP] [PMID 22331846]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IMP] [PMID 12482937]; GO_process: GO:0030466 -
chromatin silencing at silent mating-type cassette
[Evidence IDA] [PMID 21818277]; GO_process: GO:0006302 -
double-strand break repair [Evidence IGI] [PMID 15725626];
GO_process: GO:0006302 - double-strand break repair
[Evidence IMP] [PMID 16418532]; GO_process: GO:0006302 -
double-strand break repair [Evidence IMP] [PMID 17538013];
GO_process: GO:0006406 - mRNA export from nucleus
[Evidence IMP] [PMID 8565072]; GO_process: GO:0031990 -
mRNA export from nucleus in response to heat stress
[Evidence IMP] [PMID 18258809]; GO_process: GO:0051028 -
mRNA transport [Evidence IEA]; GO_process: GO:0035392 -
maintenance of chromatin silencing at telomere [Evidence
IMP] [PMID 16418532]; GO_process: GO:0031081 - nuclear
pore distribution [Evidence IMP] [PMID 8565072];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 22110603]; GO_process: GO:0045893 - positive
regulation of transcription, DNA-templated [Evidence
IDA,IGI,IMP] [PMID 15817685]; GO_process: GO:0000973 -
posttranscriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [Evidence IMP] [PMID 20932479];
GO_process: GO:0006606 - protein import into nucleus
[Evidence IMP] [PMID 12730220]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0034398 -
telomere tethering at nuclear periphery [Evidence IMP]
[PMID 16418532]; GO_process: GO:0006810 - transport
[Evidence IEA,IEA]"
/codon_start=1
/product="Nup84p"
/protein_id="XP_018733618.1"
/db_xref="GeneID:30036017"
/translation="MGASALSTGLGANKGLFNNTGSRFRTDTSGISKDPNLVTELDAD
APLRSKKTLCPEDEEADRIAFKYVFELLRARKYEEALDICEKTGNWSLKVTISGHNDY
IDPEIDGGLLSNIDDSEPRGAEAKVLWQYMCRQLARQAQNIDAYERGIYGFLGGDLDS
VLKLCDTWETQFIAYMSHLMVCDADAALVSDKRLAPGLAISPSAIKANHSSSSAQKVL
DILSNSSNPVVAVQSEHMIRTLMGAVINGTVGYLVEEASGILRQVMTGAQGSSPLTDN
PYILRIFVHLILFLQSIDVPVGDAENVSVILCGYIELLRLQNMGTIAPLYIAYLPDDI
AIDTYSVLLATITEQDIRKEHLQLGHKYGLDMANTIRAAVERVFSENEHLYDDQEFTD
ISTTVSESDVKLYQSIEWFIDSNMWPDAVHSMAALYRRFLSAGRVGAAREFGNRISVS
YVVKRYDGHMIGSGHSLTKVSTDSQNELAGIERIQVLEFERLLNCINDIAKWDLHVQV
PDQSSKPWRMDALAIVDSISSQVYDLCLNWFRQEEFELDQRIQRLRSIYLPYIILELG
RVLISAQKVHSGFLKQAAELAVVIADEKQGLYRLFLASNQLESLLTIVATAVTDGMAR
GESGIFDA"
gene complement(<754109..>754190)
/locus_tag="AWJ20_3941"
/db_xref="GeneID:30036018"
tRNA complement(<754109..>754190)
/locus_tag="AWJ20_3941"
/product="tRNA-Ser"
/db_xref="GeneID:30036018"
gene complement(<754376..>756945)
/gene="TOP1"
/locus_tag="AWJ20_3942"
/db_xref="GeneID:30036019"
mRNA complement(join(<754376..756696,756882..>756945))
/gene="TOP1"
/locus_tag="AWJ20_3942"
/product="DNA topoisomerase 1"
/transcript_id="XM_018880983.1"
/db_xref="GeneID:30036019"
CDS complement(join(754376..756696,756882..756945))
/gene="TOP1"
/locus_tag="AWJ20_3942"
/inference="similar to AA sequence:KEGG_Orthology:K03163"
/note="Topoisomerase I; nuclear enzyme that relieves
torsional strain in DNA by cleaving and re-sealing the
phosphodiester backbone; relaxes both positively and
negatively supercoiled DNA; functions in replication,
transcription, and recombination; role in processing
ribonucleoside monophosphates in genomic DNA into
irreversible single-strand breaks; GO_component:
GO:0005694 - chromosome [Evidence IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IPI] [PMID 10967121];
GO_component: GO:0005654 - nucleoplasm [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
6088500]; GO_component: GO:0031298 - replication fork
protection complex [Evidence IDA] [PMID 16531994];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0003677 - DNA binding [Evidence IEA,IEA];
GO_function: GO:0003916 - DNA topoisomerase activity
[Evidence IEA]; GO_function: GO:0003917 - DNA
topoisomerase type I activity [Evidence IEA,IEA];
GO_function: GO:0003917 - DNA topoisomerase type I
activity [Evidence IMP] [PMID 2989818]; GO_function:
GO:0003917 - DNA topoisomerase type I activity [Evidence
IDA] [PMID 6088500]; GO_function: GO:0003918 - DNA
topoisomerase type II (ATP-hydrolyzing) activity [Evidence
IEA]; GO_function: GO:0031490 - chromatin DNA binding
[Evidence IBA]; GO_function: GO:0016853 - isomerase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006200
- ATP catabolic process [Evidence IEA]; GO_process:
GO:0006271 - DNA strand elongation involved in DNA
replication [Evidence IMP] [PMID 2549254]; GO_process:
GO:0006265 - DNA topological change [Evidence IEA];
GO_process: GO:0006265 - DNA topological change [Evidence
IMP] [PMID 2989818]; GO_process: GO:0006265 - DNA
topological change [Evidence IDA] [PMID 6088500];
GO_process: GO:0006333 - chromatin assembly or disassembly
[Evidence IMP] [PMID 9199287]; GO_process: GO:0000183 -
chromatin silencing at rDNA [Evidence IMP] [PMID
10082585]; GO_process: GO:0007076 - mitotic chromosome
condensation [Evidence IGI,IMP] [PMID 8895658];
GO_process: GO:0007097 - nuclear migration [Evidence
IGI,IMP] [PMID 8895658]; GO_process: GO:0000019 -
regulation of mitotic recombination [Evidence IMP] [PMID
2902925]; GO_process: GO:0006357 - regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 1660829]; GO_process: GO:0006368 -
transcription elongation from RNA polymerase II promoter
[Evidence IMP] [PMID 2840207]"
/codon_start=1
/product="DNA topoisomerase 1"
/protein_id="XP_018733619.1"
/db_xref="GeneID:30036019"
/translation="MPEPIMMDSDMSDDDIPLVSRVASYVEVSDDDDDDDLVLAKRTT
TATKRKAISVKTEVNGSPPTKKRASTTVKKEKTTTASKAKKASTATVNGKAKKEVTSK
TNGRVKKETGSTASGKTKKEPGAGSKSATIKKEEEEEGTPAIDDEDQDETFRWWEAQN
AEDGSDKWNTLEHNGVMFPPPYEPLPSHVRMKYDGKRVELDPVAEEVAGFFGAMLNTD
HAKNPTFTANFFNDFVEIVNSSGGAKVNNKKIELKSFEKCDFTEIFEYYDQQRQLKKA
IPSSEKKRLKAEKDRMEEKYKTCLLDGRKQPVGNFRIEPPGLFRGRGAHPKTGKLKAR
VQPEQVTINIGAEAKVPEPPAGHQWAEVRHDKTVSWLAMWRENINGAFKYVFLAQSSS
LKGMSDFKKFEKARELKNHVERIRADYRRELKDEVMLNRQRATAMYLIDVLALRAGGE
KGEDEADTVGCCSLRYEHVTLQPPNIVTFDFLGKDSIRYFQEVVVDKQVFKNLKIFKR
APKTIGDDIFDRLDPPLLNKHLQNYMPGLTAKVFRTYNASYTMQKQLDLIENKGSVNE
KVVAFNAANREVAILCNHQRTVAKSHEASVGKMEDRILELKYSIIKTKKMMLQLDPSL
KKKEKAYFEDIDSISKEDVIKIHQKILEREKDRIEKKFIRENEKLKAEGEEIMPEGEL
KKRLEAIDIMEKEFAAELKSGKPDIKGNATVEKLKVQVEKLEERITNTQLQLKDKEDN
STVALGTSKMNYIDPRLTVMFSKKFNVPIEKLFTKTLREKFAWAIHSADADWKF"
gene complement(<757564..>758625)
/gene="CBP3"
/locus_tag="AWJ20_3943"
/db_xref="GeneID:30036020"
mRNA complement(<757564..>758625)
/gene="CBP3"
/locus_tag="AWJ20_3943"
/product="Cbp3p"
/transcript_id="XM_018880984.1"
/db_xref="GeneID:30036020"
CDS complement(757564..758625)
/gene="CBP3"
/locus_tag="AWJ20_3943"
/inference="similar to AA sequence:KEGG_Orthology:K17662"
/note="Mitochondrial protein required for assembly of
cytochrome bc1 complex; forms a complex with Cbp6p that
binds to mt ribosomes near the polypeptide tunnel exit and
promotes efficient translation of the COB mRNA;
Cbp3p-Cbp6p complex also interacts with newly synthesized
cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp
into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is
sequestered if assembly of Complex III is blocked,
downregulating COB mRNA translation; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 23509072]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0032592 - integral component of mitochondrial membrane
[Evidence IDA] [PMID 11522252]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0031966 -
mitochondrial membrane [Evidence IEA]; GO_component:
GO:0005761 - mitochondrial ribosome [Evidence IDA] [PMID
21670217]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 11522252]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0043022 - ribosome binding [Evidence IDA,IPI] [PMID
21670217]; GO_process: GO:0034551 - mitochondrial
respiratory chain complex III assembly [Evidence IMP]
[PMID 11522252]; GO_process: GO:0034551 - mitochondrial
respiratory chain complex III assembly [Evidence IMP]
[PMID 15759116]; GO_process: GO:0070131 - positive
regulation of mitochondrial translation [Evidence IMP]
[PMID 21670217]"
/codon_start=1
/product="Cbp3p"
/protein_id="XP_018733620.1"
/db_xref="GeneID:30036020"
/translation="MAGVFVGSLRNAGRLAINNSISARSAGASLRAGVSTISSSHNGR
FGIRGYSSTPAEDASKEKGADAVSEPATPTPVTTPPFIAKPGSLKDQILKQYQVVDST
YNVKLPAWQNMLGENLVKTFNLDMDRIRSGPVAGALFKDMCKAQAFYQKEGDKITMTP
RAKFYYETLGMPQTFNQWYQVTLLHVWILFVRMRSMPRKYCREYQQKLVNSIFEDIDF
RLREEIKINSDRMVNNYKKGFNDQLRGSIFAYDEGFYSDDTVLAGALWRNLFESKKDI
DMTHIEQLVHYVRTQLYVLDRMSDLDFAAGRFQFIDPSLRYQPLTEGEDKEIKSIVES
NRKESTDAASRSTLSKEGW"
gene <758904..>759806
/gene="EMC2"
/locus_tag="AWJ20_3944"
/db_xref="GeneID:30036021"
mRNA <758904..>759806
/gene="EMC2"
/locus_tag="AWJ20_3944"
/product="Emc2p"
/transcript_id="XM_018880985.1"
/db_xref="GeneID:30036021"
CDS 758904..759806
/gene="EMC2"
/locus_tag="AWJ20_3944"
/note="Member of conserved ER transmembrane complex;
required for efficient folding of proteins in the ER; null
mutant displays induction of the unfolded protein
response; homologous to worm Y57G7A.10/EMC-2, fly CG17556,
human TTC35; GO_component: GO:0072546 - ER membrane
protein complex [Evidence IDA] [PMID 19325107];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA,IEA]; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IDA] [PMID 14562095]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0034975 -
protein folding in endoplasmic reticulum [Evidence IGI]
[PMID 19325107]"
/codon_start=1
/product="Emc2p"
/protein_id="XP_018733621.1"
/db_xref="GeneID:30036021"
/translation="MSSETKEKLLTSMANASYYNASPRDLESLHAESRQYLSKAQHLI
PEEQLLGLLEQHFYLALLTCKDSEAKLVLQRLTDRITEDPSRVAILKAQYIEATDGPE
AAQKFLSARPTGDLRAFKRKTVILKQKGDIKTYIKELLRYVEEVAPNDSEAWAELAEA
YVSIGQYPQAIHALEEVLISAPQASNIFARLGEVFLIEATTSSPNKVTSDQLTLLIHS
AQHFLRSVELSPDYIRGWCGALIASSKILAWPKLAAEESTKYTKISKIAKKQTQRLIK
TAKEAAISDEDIAAAKIIIAQYEN"
gene complement(<760023..>760913)
/gene="TIP41"
/locus_tag="AWJ20_3945"
/db_xref="GeneID:30036022"
mRNA complement(<760023..>760913)
/gene="TIP41"
/locus_tag="AWJ20_3945"
/product="Tip41p"
/transcript_id="XM_018880986.1"
/db_xref="GeneID:30036022"
CDS complement(760023..760913)
/gene="TIP41"
/locus_tag="AWJ20_3945"
/inference="similar to AA sequence:KEGG_Orthology:K17607"
/note="Protein that interacts with Tap42p, which regulates
PP2A; component of the TOR (target of rapamycin) signaling
pathway; protein abundance increases in response to DNA
replication stress; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11914276]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0009968 - negative regulation of
signal transduction [Evidence IEA]; GO_process: GO:0007165
- signal transduction [Evidence IMP,IPI] [PMID 11741537]"
/codon_start=1
/product="Tip41p"
/protein_id="XP_018733622.1"
/db_xref="GeneID:30036022"
/translation="MDHITDKENRQGVPVFQTPRNGFAVPSPSQTTRVWTSPDNEWKV
TTVKKPILNSSEIDAATEKLGIPIPEMIFGNNQVKIENVPSGLCICFSALDALDLVDK
TGSRDGLVKVAYAKEWMDSREKSPHINDDVKGIVKPFDWTYTSAYKGDTTVKGVLADP
WVEDKDVILPMELLSRPDPILFFDDVCLYEDELGDNGIANFSVKIRVMPERLLLLSRL
FIRVDDVLFRVRDTRIFVEFSQGVLVREYKAHEETYDKVKKRVPIGSQDYSQNLRDSN
WVVKNIACSKTVHEIIELRK"
gene complement(<765914..>767086)
/locus_tag="AWJ20_3946"
/db_xref="GeneID:30036023"
mRNA complement(<765914..>767086)
/locus_tag="AWJ20_3946"
/product="hypothetical protein"
/transcript_id="XM_018880987.1"
/db_xref="GeneID:30036023"
CDS complement(765914..767086)
/locus_tag="AWJ20_3946"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733623.1"
/db_xref="GeneID:30036023"
/translation="MKSSKIFPFKGERKKGGDGVELNREIANVEAPIAQITSTHAGSM
LGHRTEQKYPPLESIISNQSSAPYTLSNLQEYLRTIHCQEPLDLILMGYQFSTLFTSD
ETSISIAEKADKINEVRQLWNKIVSTFLLDSSPQEVNLPGELREHFYLIPVSQSVTTE
NYKHLQQAIEYAKDIIKENNFMLFIRNTQTMGALKANTRAKIQLNTSFMDQDHSIGHG
RGYSIGSSPTLNNPPHPLGCPQSNERSLRAASLAVPSKRNHRCISPTFPQIVDDGNLI
TTTTTSTFPSTLNANVEEDISRWTLQEPVRMFNRSPSPGTGKSPGASPGRTISPSPTP
SPRLSPVPASKSKKDLKNGPGVDSVTAVNASTFPHLLKRMSGKLKIRQQSSAHTPP"
gene <769234..>770232
/gene="GOR1"
/locus_tag="AWJ20_3947"
/db_xref="GeneID:30036024"
mRNA <769234..>770232
/gene="GOR1"
/locus_tag="AWJ20_3947"
/product="Gor1p"
/transcript_id="XM_018880988.1"
/db_xref="GeneID:30036024"
CDS 769234..770232
/gene="GOR1"
/locus_tag="AWJ20_3947"
/note="Glyoxylate reductase; null mutation results in
increased biomass after diauxic shift; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0051287 -
NAD binding [Evidence IEA]; GO_function: GO:0048037 -
cofactor binding [Evidence IEA]; GO_function: GO:0047964 -
glyoxylate reductase activity [Evidence IEA]; GO_function:
GO:0047964 - glyoxylate reductase activity [Evidence IMP]
[PMID 17173333]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA]; GO_function: GO:0016616 -
oxidoreductase activity, acting on the CH-OH group of
donors, NAD or NADP as acceptor [Evidence IEA];
GO_function: GO:0016616 - oxidoreductase activity, acting
on the CH-OH group of donors, NAD or NADP as acceptor
[Evidence ISS] [PMID 9341119]; GO_process: GO:0009436 -
glyoxylate catabolic process [Evidence IMP] [PMID
17173333]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence ISS] [PMID 9341119]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="Gor1p"
/protein_id="XP_018733624.1"
/db_xref="GeneID:30036024"
/translation="MTAIKNQILLLGSVSLAKKEYQELSELAELISYTSSSREEFIAD
LKTKYKEITAIYSTFAGEKAVGGIDEEIVSHFPPSLKYICHNGAGYDLVQVEYLTKRG
IQLSNTPDAVANATADTAVYLILSALRNFSRLSEELRKGNWLGTVPLAREPKDKVLGI
LGMGSIGHLVHEKVIPLGFSKVIYYNRHQLPKEKEKGAEYVTFDELLAQSDVLFVSVP
LNAGTRHIINAEALAKAKDGVVVVNTARGAVIDEEALVQALESGKVSNVGLDVFEFEP
KIHPGLLANPNAVLLPHVGTHTVESRTEMELTVIRNIRSALTTGKVIDVVPEQQKK"
gene complement(<770433..>771488)
/locus_tag="AWJ20_3948"
/db_xref="GeneID:30036025"
mRNA complement(<770433..>771488)
/locus_tag="AWJ20_3948"
/product="hypothetical protein"
/transcript_id="XM_018880989.1"
/db_xref="GeneID:30036025"
CDS complement(770433..771488)
/locus_tag="AWJ20_3948"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733625.1"
/db_xref="GeneID:30036025"
/translation="MASSKDIHSTLLAEIANLLKILDHEGGNLDHAVTHKVENSLNAI
KDISSLWQAHTTKLALAFKPPISGEAALKCLEEAGKLPRLLVAAYNAIDEDDKTGLMK
PEVKDHIDMLFLAENSFLEELKEAVVRERLLLQNLRPASADSNARTLVSVGEVWESCK
KLQALIDNGPVAVLQGKITAYSGIIRDAVEEFKELMEEEREDSDDENNDDDVDKPPSA
VSDSEEVDDFEEFNFSAPSKPLSKELRQVGIKWKDMFIRLPTVTEVLCKTLCKRLENT
ESTKIIKINTCIKSISEATDDLVFGFSDNSITDVKEGFVVVTKELETIADLTTRPDDN
FSTQLRAYIEKFKANNS"
gene complement(<776972..>777658)
/locus_tag="AWJ20_3949"
/db_xref="GeneID:30036026"
mRNA complement(<776972..>777658)
/locus_tag="AWJ20_3949"
/product="hypothetical protein"
/transcript_id="XM_018880990.1"
/db_xref="GeneID:30036026"
CDS complement(776972..777658)
/locus_tag="AWJ20_3949"
/note="expressed protein"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733626.1"
/db_xref="GeneID:30036026"
/translation="MPLEQRYDTLQDNYIVRLHRNVDPENPVNAESTWWRKHMEHNEL
VQRLYEVLPKEEIFQDRIRRRKPPWKKEDMECESKAWKVKSELKKKIYQRHHTIWETQ
YRTSAKGEFYRSYTLPRLYNADHKNPLRYFLNECSKNELSKLVQLRTAKGAFGMFFKR
FKINNRPHQCECGEEEDVKHLLCECPVTENHRQILRDASAMLDLKVLLDSKKGLKAVL
AFLAKAPQLL"
gene <781841..>782137
/locus_tag="AWJ20_3950"
/db_xref="GeneID:30036028"
mRNA <781841..>782137
/locus_tag="AWJ20_3950"
/product="hypothetical protein"
/transcript_id="XM_018880992.1"
/db_xref="GeneID:30036028"
CDS 781841..782137
/locus_tag="AWJ20_3950"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733627.1"
/db_xref="GeneID:30036028"
/translation="MDPNSQPAFSSTVYFVPSGSSGHGLNLVKTLASVGNSVVITTAA
NPEMSIDLQNWVKNHSNVHVLKYDGSNESDADDVANQIIELTGGIDVVVNTRTW"
gene <783804..>787265
/gene="PBP1"
/locus_tag="AWJ20_3951"
/db_xref="GeneID:30036029"
mRNA <783804..>787265
/gene="PBP1"
/locus_tag="AWJ20_3951"
/product="Pbp1p"
/transcript_id="XM_018880993.1"
/db_xref="GeneID:30036029"
CDS 783804..787265
/gene="PBP1"
/locus_tag="AWJ20_3951"
/note="Component of glucose deprivation induced stress
granules; involved in P-body-dependent granule assembly;
similar to human ataxin-2; interacts with Pab1p to
regulate mRNA polyadenylation; interacts with Mkt1p to
regulate HO translation; protein increases in abundance
and relative distribution to the nucleus increases upon
DNA replication stress; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11914276]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
22842922]; GO_component: GO:0010494 - cytoplasmic stress
granule [Evidence IDA] [PMID 18981231]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 11914276]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_component: GO:0005844 - polysome [Evidence IDA] [PMID
15082763]; GO_function: GO:0003729 - mRNA binding
[Evidence IDA] [PMID 23222640]; GO_process: GO:0006378 -
mRNA polyadenylation [Evidence IGI,IMP,IPI] [PMID
9819425]; GO_process: GO:0045727 - positive regulation of
translation [Evidence IMP,IPI] [PMID 15082763];
GO_process: GO:0034063 - stress granule assembly [Evidence
IMP] [PMID 18981231]"
/codon_start=1
/product="Pbp1p"
/protein_id="XP_018733628.1"
/db_xref="GeneID:30036029"
/translation="MSTTASRGGRQPSVSGRNGSNGGGRRFNGGGGGNSGQGSSSSAN
GNGINSGTNLAAVDPPLVRGGPGGRASLSGPSTTSGPGSNATAGSGSGAGSRGSGGIG
PKNAWSKPLQSSVTTTSGGSSGSGTTVSNNNSNSNSGALSNVAGSNESNSQNHSSAAA
ATPASSAFSSGSTESVASGDPEKHMNDRLTFLLAKSIGCTAIVTVASGHRYKGILAGA
LTDSDLGVVLRFAEKTSSVPGIEDEDAAADPNGNSGTSGTTGNKASGNNSTSAAGDDG
KNKRNGSEEQKRFDKLVIAPKDCVDIFIENPDLTPDVKSETPANSVSSGINKSSGAFK
TDTDISGRRNGLGGERELQRWTPDSNIELGVTLEETAGSNSGGWDQFSVNKQKFGVAS
TYDEHYYTTAIDKSHPEYQERARRAEQIAAEITKSGHGGNVHLAEERGLAIDDSGLDE
EDKYSGVDRGQQKRDSLGQTSGQSQRVPVQKLFQQAHREKLQGQGQAQTGYEPDQRQT
PSPPVPSGSTKAKKYTPPQFRAPTGVKGVPGVPYDPAIVSSSLASPDGSLAPRTQDMS
ARVGKSDDKSNTPTAVAPAATASATASAAPVVQAANSEGANASSNTSASDSPSGGIEK
ELAGNFRQFVSVEVQRLNQKKQYLQKREKSERLHDFKKFSEDYKINAPVPLDLVPILA
KGKDKQEEIVQRAAANAAAAHSPKTSPSTSKTSTVAPALPATGSPRGTSTESSPAITN
NTHPSTLDGATSTGTKAVLPNKPTTKPSTVQPTPAVPAVPVPVSTAPVTTSAPASTPA
TGTPVPSAAAAEKPKFNFKVPGFKPNPSAHSFTPFGAKGSPSPVASQISSPQTKVTAP
VPETGSATGRAPVATATANSATSGTATPNSFFGGRQPGAKSRQNKFNPFLEAKSHHKP
DETFTIERAFATPPTWTPGVEKSYSEVLTSLKISSPRIGGFFPPGSGGIGSPRPSVTG
FAPSASPMGMSGAANLVAMMPATAFDEVGNPIMTTSPPPIPGQMMPAGMVPVGYPQYV
PQYGAPQYYGRPPPMGFPMGYLPPQAGYGSPRMHQAMMVPPGQPSNGYYPPQHQFIPQ
QRTHHNNNNNYSRRGSAGPGNHGGGGGGPPTSLPPQMQGQFPPPPASNALNSKSPTPT
TPVEEEK"
gene <787754..>789199
/locus_tag="AWJ20_3952"
/db_xref="GeneID:30036030"
mRNA <787754..>789199
/locus_tag="AWJ20_3952"
/product="hypothetical protein"
/transcript_id="XM_018880994.1"
/db_xref="GeneID:30036030"
CDS 787754..789199
/locus_tag="AWJ20_3952"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733629.1"
/db_xref="GeneID:30036030"
/translation="MDKLITELIEQPKEGSHFRDPGLGPLPSAQNLDKARKVIAETSN
KLPLEGLGIEKVAGILTNDILPALPNQSSHYYGFVTGGATPAARLADHLAVWADTMPQ
VHLPEESVSTDVEDAALKLLLDLLKLERTQWLGRTITTGATASNVLALACARDWLVGS
DEASIAEHGFANALKASGVQDIKIYVCKSHSSIGKAASIVGLGRKSVVEISDAAHLWN
FDIAKLEEQLISDKSKSIRSIICANFGEVNTGQYTQDIKELRRLCDQYKAWLHIDGAF
GVYLRALADKYDSAYHQLAQRVTAGLELADSITGDSHKTLNVPYDSGVFFTKHKELLQ
HIFHNPGAVYLSGTSDSIPSPLNIGIENSRRFRALAVYSTLLAYGEAGYSQFIERIIR
FSQRLSAWIHQSPDYELLLESPKDAISVVLFRAKDTEANKTLKQRINQSGKIYVSGTV
WNGSPAVRIAPCNWNIADGDEQIVINVLSTF"
gene <789406..>791118
/gene="RRN7"
/locus_tag="AWJ20_3953"
/db_xref="GeneID:30036031"
mRNA <789406..>791118
/gene="RRN7"
/locus_tag="AWJ20_3953"
/product="Rrn7p"
/transcript_id="XM_018880995.1"
/db_xref="GeneID:30036031"
CDS 789406..791118
/gene="RRN7"
/locus_tag="AWJ20_3953"
/inference="similar to AA sequence:KEGG_Orthology:K15219"
/note="Component of the core factor (CF) rDNA
transcription factor complex; CF is required for
transcription of 35S rRNA genes by RNA polymerase I and is
composed of Rrn6p, Rrn7p, and Rrn11p; GO_component:
GO:0070860 - RNA polymerase I core factor complex
[Evidence IDA] [PMID 7958901]; GO_component: GO:0070860 -
RNA polymerase I core factor complex [Evidence IDA] [PMID
8702872]; GO_component: GO:0070860 - RNA polymerase I core
factor complex [Evidence IDA] [PMID 8887672];
GO_component: GO:0005730 - nucleolus [Evidence IEA];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
22362748]; GO_component: GO:0005634 - nucleus [Evidence
IEA]; GO_function: GO:0003677 - DNA binding [Evidence
IEA]; GO_function: GO:0001164 - RNA polymerase I CORE
element sequence-specific DNA binding [Evidence IDA] [PMID
18451108]; GO_function: GO:0001187 - RNA polymerase I CORE
element sequence-specific DNA binding transcription factor
recruiting transcription factor activity [Evidence IC];
GO_function: GO:0017025 - TBP-class protein binding
[Evidence IDA,IPI] [PMID 8702872]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_process: GO:0006355
- regulation of transcription, DNA-templated [Evidence
IEA]; GO_process: GO:0006361 - transcription initiation
from RNA polymerase I promoter [Evidence NAS] [PMID
7958901]; GO_process: GO:0042790 - transcription of
nuclear large rRNA transcript from RNA polymerase I
promoter [Evidence IDA,IMP] [PMID 7958901]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Rrn7p"
/protein_id="XP_018733630.1"
/db_xref="GeneID:30036031"
/translation="MSSIWVRGPVCGVDNCRSRLWRSVDGRKVCQYGHVKEGIEVGDD
DDDFLAFGRVISRPSSRRNSSAQEKEHALIGAAAQALYFQCLQVVLRQQVKWLIEEKG
VPSELEGVVRSLWTILVNQTKFGVSNMNSSRASSAAKSDASQDESEKYTTHNDEDTGN
DEDEDDYDDDGDEENNYRNDSDTERERRGRRKIKTEKLPEIKFGHLVVICYLGCVTIR
SPVYLYDFQKWIERLEFPFIRALAYLPLEMSQKVVGTHYRGLLSPEIPRDGKLHGLLA
ELGSFYIHSGVTFPPQPVEPLLFRMIKDLLLPPEIYVASEKLAEALEISLLVSISNHY
YPQFETALFGVIIIAVRLCYGVGDEWTRVPKSYSTPASKTIDWDLWCDIMRKIWVEDD
EFFDVNESDICYWDPEKSERYLSWVENSLLILPRFRSEYKRLLQLFPLGKYDKSGNPM
STKAFEPRQGHPRSDLAIHAGEKEIEELYRIVQSTTRNVECHYPEDVLDKIKVKGVLL
SPGARFETYRQNDKLPLFMTIVCHVGSKLCGVSFDSVKRLIHDLDRKCNKLDFSAMSE
QSFY"
gene complement(<791308..>792243)
/gene="CDC21"
/locus_tag="AWJ20_3954"
/db_xref="GeneID:30036032"
mRNA complement(<791308..>792243)
/gene="CDC21"
/locus_tag="AWJ20_3954"
/product="thymidylate synthase"
/transcript_id="XM_018880996.1"
/db_xref="GeneID:30036032"
CDS complement(791308..792243)
/gene="CDC21"
/locus_tag="AWJ20_3954"
/inference="similar to AA sequence:KEGG_Orthology:K00560"
/note="Thymidylate synthase; required for de novo
biosynthesis of pyrimidine deoxyribonucleotides;
expression is induced at G1/S; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 8132557]; GO_function:
GO:0008168 - methyltransferase activity [Evidence IEA];
GO_function: GO:0004799 - thymidylate synthase activity
[Evidence IEA,IEA]; GO_function: GO:0004799 - thymidylate
synthase activity [Evidence IMP] [PMID 334734];
GO_function: GO:0004799 - thymidylate synthase activity
[Evidence IDA] [PMID 6287238]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0006231 - dTMP biosynthetic process [Evidence IEA];
GO_process: GO:0006231 - dTMP biosynthetic process
[Evidence IMP] [PMID 334734]; GO_process: GO:0006231 -
dTMP biosynthetic process [Evidence IMP] [PMID 794696];
GO_process: GO:0006235 - dTTP biosynthetic process
[Evidence IEA]; GO_process: GO:0032259 - methylation
[Evidence IEA]; GO_process: GO:0009165 - nucleotide
biosynthetic process [Evidence IEA]"
/codon_start=1
/product="thymidylate synthase"
/protein_id="XP_018733631.1"
/db_xref="GeneID:30036032"
/translation="MTSDESLTTGGPVKTNTENVEERAYLDLCKDIIEQGEFRADRTG
TGTYSLFAPNQLRFSLRNGTLPLLTTKRVYFKGVLEELLWFVKGSTDSKLLSDRGVHI
WDGNGSREFLDKRGLEHRREGDLGPVYGFQWRHFGAKYEDCDTDYSGKGVDQLAEVIR
KIKETPYDRRIIMSAWNPADMNDMALPPCHVFSQFYVSFRNGKPELSCQLYQRSCDMG
LGVPFNIASYALLTKMIAKVCGIECGDFIHTMGDAHVYADHVEALKVQIARTPTDFPT
LRFARDITDIDDFKYEDFVVEGYKPQSKIEMKMSV"
gene complement(<792503..>792573)
/locus_tag="AWJ20_3955"
/db_xref="GeneID:30036033"
tRNA complement(<792503..>792573)
/locus_tag="AWJ20_3955"
/product="tRNA-Gly"
/db_xref="GeneID:30036033"
gene <798789..>802895
/gene="GIP3"
/locus_tag="AWJ20_3956"
/db_xref="GeneID:30036034"
mRNA join(<798789..799029,799108..>802895)
/gene="GIP3"
/locus_tag="AWJ20_3956"
/product="protein phosphatase regulator GIP3"
/transcript_id="XM_018880997.1"
/db_xref="GeneID:30036034"
CDS join(798789..799029,799108..802895)
/gene="GIP3"
/locus_tag="AWJ20_3956"
/codon_start=1
/product="protein phosphatase regulator GIP3"
/protein_id="XP_018733632.1"
/db_xref="GeneID:30036034"
/translation="MTANESTLNGVDHSSPINISPSARNPLLDSTSSLSRSVTSSQLE
LEPTHNGVSNTDVDWLSRGSSLKDRGQYPLPNSVAGGRVRTRSNSLPPVPSGERLPPQ
LGRSKATTGVSVEGGQASSKELGGSDQSGENIINNKKKIPAAAAAASTIEEDEEAEES
SEKGPPLSPTSTTLRNPAPGPGYANQAVTAHSKLSQGLRSSKTMPIALEKTQVGSELT
RSISVSSAALGKSPSDKLPSTSPSNARRGSWFHNLSSKFSSSSSHNNNGSSVVSNQRR
ASTSGLKNTVPGSTLSTGSVKDSPSSGLGLDVESHSNLAHAHVAGGNIPPSANPIFSP
SYTNNRNLPTNRILDTHPPRTSSPKLSPEIAVGGTAQSPSPSSAFPFKQEHNRSNSVS
STGSLVNRLRRLSTRSNGSQSDLISTQFKAESQQVRVILNKNHSKKPCKIPELEGVLP
PTVRFKAEVFETDPPQQIPARNPRTGNITFGPNGEINREPLPPNGYPMPLYVLPRNYG
ASTLAASQSAYESAIRVANSVKSSGGVSIFKSRNSLSSTTSFSSNNTEYDEEKDVSES
DRTQSLKIDKPMHSAERMGSGSTNTTTASTGDSSVEDDDLDEQGKELTLETIYTRCCH
LREILPIAATLKQIKDRKPPLEVIKMMNPRPTLIEILSLSDFLSVVPVRMLIFDNVSL
STEMFRIILSSVVTSDHLERLSLRNLILDDKGWKLFCAFFSENSSLVKLDLSIEKVNS
KQPSDRSLLDWDLFTKALVARGGVEELILNGCVVPSDELAPLIFNGCSLSTKRLGLAL
NDLLPEDLQVIMKWAADPSCVCEGLDLGGNLMDQSWDAIKEILKGKCLKFLSLNSTGL
ANVTKANEIFGGVCSHSNLIMLDLSSNPDLFPKFTHVLAKVLPTFTDLRRIHLESNNL
SPEDVMLLADAFARCPKLVHISVLDNRRLDSTACAALAIATHISNTIYRVESDNDLWP
VILQRRLAHNCLVNMELTAGRITQQEKDSDDDGLFGKEDLMNTGNYIAMAAEELLKSI
KEDDDNDSEDAQGHLVVSEMVRRAKQVRVKIRKTLDELFEKRRSGVGLVVEEKERLVR
LCFLDGTLEKVLDKYNQTKLYKHENQNEESTKGSGNDVSTQNRMGTTSLSGKSVPPLN
MPPLLPVDREVVLSTTVSTHPDFTIAPVSEYPEVQSLSRRSSVTSLHRKEQELEEGEL
HRFGTFIRSHRDSHTDLSDDDEDEDAHVVDSEEDIRRRSSDEGTGEELRQKILAAKGS
QSVSELIQQLQQMHGPELDRILAAGKDKSLSERDDILLRLNHAARPRSAQASTPPEDT
KLLAGDYVTLDSVVDDIARALT"
gene complement(<803096..>803986)
/gene="RPL5"
/locus_tag="AWJ20_3957"
/db_xref="GeneID:30036035"
mRNA complement(<803096..>803986)
/gene="RPL5"
/locus_tag="AWJ20_3957"
/product="ribosomal 60S subunit protein L5"
/transcript_id="XM_018880998.1"
/db_xref="GeneID:30036035"
CDS complement(803096..803986)
/gene="RPL5"
/locus_tag="AWJ20_3957"
/inference="similar to AA sequence:KEGG_Orthology:K02932"
/note="Ribosomal 60S subunit protein L5; homologous to
mammalian ribosomal protein L5 and bacterial L18; binds 5S
rRNA and is required for 60S subunit assembly;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0022625 - cytosolic large ribosomal
subunit [Evidence IDA] [PMID 11983894]; GO_component:
GO:0005622 - intracellular [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0005840 - ribosome [Evidence IEA,IEA];
GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
GO_function: GO:0008097 - 5S rRNA binding [Evidence IDA]
[PMID 8764831]; GO_function: GO:0003723 - RNA binding
[Evidence IEA]; GO_function: GO:0019843 - rRNA binding
[Evidence IEA]; GO_function: GO:0003735 - structural
constituent of ribosome [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IC] [PMID 11983894]; GO_process: GO:0002181 - cytoplasmic
translation [Evidence IC] [PMID 11983894]; GO_process:
GO:0000027 - ribosomal large subunit assembly [Evidence
IMP] [PMID 8474444]; GO_process: GO:0006412 - translation
[Evidence IEA]"
/codon_start=1
/product="ribosomal 60S subunit protein L5"
/protein_id="XP_018733633.1"
/db_xref="GeneID:30036035"
/translation="MAFQKIEKTAAYHSRYQTPFRRRREGKTDYYARKRLVTQHKAKY
NSPKYRLVIRFTKKDIIAQVVSSQITGDVVLSAAYAHELPRYGIKHGLTNWSAAYAVG
LLIARRTLQKLGLDETYQGDEEASGEYSLTEAVEDGPRPFKVFLDVGLTRTTTGNRVF
GAMKGASDGGLYIPHSAKRFPGFDIETEELDADVLRKYIVGGHVAEYMEELADDDEER
YRTLFKSYIEDDIEADGVEDIYTEAHEKIRADPSFVPSNKKSKEEYKAISLKYKQKKL
TSEERKAKVAAKIAEFQANN"
gene complement(<807826..>808116)
/locus_tag="AWJ20_3958"
/db_xref="GeneID:30036036"
mRNA complement(<807826..>808116)
/locus_tag="AWJ20_3958"
/product="putative amidotransferase"
/transcript_id="XM_018880999.1"
/db_xref="GeneID:30036036"
CDS complement(807826..808116)
/locus_tag="AWJ20_3958"
/note="Putative glutamine amidotransferase; has
Aft1p-binding motif in the promoter; may be involved in
copper and iron homeostasis; YLR126C is not an essential
protein; relocalizes from nucleus to cytoplasmic foci upon
DNA replication stress; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 22842922];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22842922]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0006878 - cellular
copper ion homeostasis [Evidence IEP,ISS] [PMID 14534306];
GO_process: GO:0006879 - cellular iron ion homeostasis
[Evidence IEP,ISS] [PMID 14534306]; GO_process: GO:0006541
- glutamine metabolic process [Evidence IEA]"
/codon_start=1
/product="putative amidotransferase"
/protein_id="XP_018733634.1"
/db_xref="GeneID:30036036"
/translation="MEMHRDIVPDLPINTELLFSNDICYNQGFYRKDSILTVQGHPEF
NEDIINKIVDVRADTGVISPELANDARNRSGDRNDGPGLAKVMVKFITEGLE"
gene complement(<808285..>808620)
/locus_tag="AWJ20_3959"
/db_xref="GeneID:30036037"
mRNA complement(<808285..>808620)
/locus_tag="AWJ20_3959"
/product="amidotransferase (predicted)"
/transcript_id="XM_018881000.1"
/db_xref="GeneID:30036037"
CDS complement(808285..808620)
/locus_tag="AWJ20_3959"
/codon_start=1
/product="amidotransferase (predicted)"
/protein_id="XP_018733635.1"
/db_xref="GeneID:30036037"
/translation="MSHIAILEADTPMPSVAEVHGSYGDIFKRLLLNAKLDPKSVIKA
YDVVTKQEYPNSLDGVDAILITGSKHNAFGDDEWIIKLVNFTKQAIDAGIKVVGTYRI
YSWFPASYS"
gene complement(<809345..>810463)
/locus_tag="AWJ20_3960"
/db_xref="GeneID:30036039"
mRNA complement(<809345..>810463)
/locus_tag="AWJ20_3960"
/product="hypothetical protein"
/transcript_id="XM_018881002.1"
/db_xref="GeneID:30036039"
CDS complement(809345..810463)
/locus_tag="AWJ20_3960"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733636.1"
/db_xref="GeneID:30036039"
/translation="MTVLLFMSMTLKFWQIFDYKWIVLSYIITGIFGSGVLSAVMVSS
YVTDSIKPRDRATAMGYVTACVLTGKALGPLISSHVIRCMNGKLIGVPLVACLFDAAA
LVWWKFTPESRQLSVNTKSMPMSRREEGSALSLALDRLNIFRSLYTIKNIAASQPNPA
SKNIFILISIDLLFEIIISGRANIAILYPEMKFGWTAVEIGYLQSATAVYRIVLLVLL
IPHFIKLLQKYYDEDEEELTGQIAMQEHCITRSQKVIIAIGNCFECVGYMGYGIATKG
LEYSISYFISTTGVFSKPAVQSGLLNLIPADQVGEFLGAKGVLDGITGLMFSSIGLSF
YSYTVARNPAIIFYVCTFGYLIVVLLASRLRLNRQLHL"
gene complement(<811717..>812742)
/gene="YKU70"
/locus_tag="AWJ20_3961"
/db_xref="GeneID:30036040"
mRNA complement(<811717..>812742)
/gene="YKU70"
/locus_tag="AWJ20_3961"
/product="Yku70p"
/transcript_id="XM_018881003.1"
/db_xref="GeneID:30036040"
CDS complement(811717..812742)
/gene="YKU70"
/locus_tag="AWJ20_3961"
/inference="similar to AA sequence:KEGG_Orthology:K10884"
/note="Subunit of the telomeric Ku complex
(Yku70p-Yku80p); involved in telomere length maintenance,
structure and telomere position effect; required for
localization of telomerase ribonucleoprotein to nucleus
via interaction with the TLC1 guide RNA; relocates to
sites of double-strand cleavage to promote nonhomologous
end joining during DSB repair; GO_component: GO:0043564 -
Ku70:Ku80 complex [Evidence IEA]; GO_component: GO:0043564
- Ku70:Ku80 complex [Evidence IDA] [PMID 8754818];
GO_component: GO:0005694 - chromosome [Evidence IEA];
GO_component: GO:0000781 - chromosome, telomeric region
[Evidence IEA,IEA]; GO_component: GO:0000790 - nuclear
chromatin [Evidence TAS] [PMID 10367891]; GO_component:
GO:0005635 - nuclear envelope [Evidence IDA] [PMID
10638763]; GO_component: GO:0005724 - nuclear telomeric
heterochromatin [Evidence TAS] [PMID 12080091];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0004003 - ATP-dependent DNA helicase
activity [Evidence IEA]; GO_function: GO:0003677 - DNA
binding [Evidence IEA,IEA]; GO_function: GO:0003723 - RNA
binding [Evidence IDA] [PMID 12975323]; GO_function:
GO:0003684 - damaged DNA binding [Evidence IEA];
GO_function: GO:0003684 - damaged DNA binding [Evidence
TAS] [PMID 10367891]; GO_function: GO:0004386 - helicase
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0000166
- nucleotide binding [Evidence IEA]; GO_function:
GO:0042162 - telomeric DNA binding [Evidence IEA];
GO_process: GO:0032508 - DNA duplex unwinding [Evidence
IEA]; GO_process: GO:0006310 - DNA recombination [Evidence
IEA]; GO_process: GO:0006281 - DNA repair [Evidence IEA];
GO_process: GO:0006974 - cellular response to DNA damage
stimulus [Evidence IEA]; GO_process: GO:0006333 -
chromatin assembly or disassembly [Evidence IDA] [PMID
11553718]; GO_process: GO:0006342 - chromatin silencing
[Evidence IDA,IMP] [PMID 9501103]; GO_process: GO:0030466
- chromatin silencing at silent mating-type cassette
[Evidence IGI,IMP] [PMID 18791224]; GO_process: GO:0000727
- double-strand break repair via break-induced replication
[Evidence IGI,IMP] [PMID 17321803]; GO_process: GO:0000724
- double-strand break repair via homologous recombination
[Evidence IMP] [PMID 11016833]; GO_process: GO:0006303 -
double-strand break repair via nonhomologous end joining
[Evidence IEA]; GO_process: GO:0006303 - double-strand
break repair via nonhomologous end joining [Evidence IMP]
[PMID 10908335]; GO_process: GO:0097552 - mitochondrial
double-strand break repair via homologous recombination
[Evidence IMP] [PMID 22214610]; GO_process: GO:0000723 -
telomere maintenance [Evidence IEA]; GO_process:
GO:0000723 - telomere maintenance [Evidence IMP] [PMID
10818099]"
/codon_start=1
/product="Yku70p"
/protein_id="XP_018733637.1"
/db_xref="GeneID:30036040"
/translation="MQQFDDASNIKSSRRCFIVTDNEDPSFGNSQQRTAAKTNAEELV
NKNVIIEPAFLSHGDSKGEGSNFDMSQFWDSITFTKPMDDEENADVEPVPSSYLVSDL
TLKTSLLSRRSPKRALFTNRIELAPGIEIGVKGYLTFSKQLFVKDRYVYTQGEEKQIV
KSEVKITDDESVNTVSPDEIIRAYEFGDSYIYFTPEQVNQIRNLGRPLIRIIGFKSPS
FVPPEYNKRHSMFVYPSDSKLTGSIRAFHSLYQCMRQKGKVAIAWAIIRSNSIPEPCA
LFPVNEEVETKGSLTVQVTPPGFQLVILPYADDIRARPMSTITEGKAERIYNSFNRNK
LTINSPK"
gene complement(<812890..>813144)
/locus_tag="AWJ20_3962"
/db_xref="GeneID:30036041"
mRNA complement(<812890..>813144)
/locus_tag="AWJ20_3962"
/product="ATP-dependent DNA helicase II subunit 1"
/transcript_id="XM_018881004.1"
/db_xref="GeneID:30036041"
CDS complement(812890..813144)
/locus_tag="AWJ20_3962"
/codon_start=1
/product="ATP-dependent DNA helicase II subunit 1"
/protein_id="XP_018733638.1"
/db_xref="GeneID:30036041"
/translation="MLELRHHESFDEPKSAVVLALEAVYEAIKSRLIVSPGDKSGIIL
YGTKKTSDAAFAWCNILMPLGAPSASQLRRVRRIIESKFH"
gene <814621..>820464
/gene="POL2"
/locus_tag="AWJ20_3963"
/db_xref="GeneID:30036042"
mRNA <814621..>820464
/gene="POL2"
/locus_tag="AWJ20_3963"
/product="DNA polymerase epsilon catalytic subunit"
/transcript_id="XM_018881005.1"
/db_xref="GeneID:30036042"
CDS 814621..820464
/gene="POL2"
/locus_tag="AWJ20_3963"
/inference="similar to AA sequence:KEGG_Orthology:K02324"
/codon_start=1
/product="DNA polymerase epsilon catalytic subunit"
/protein_id="XP_018733639.1"
/db_xref="GeneID:30036042"
/translation="MAYDIETTKAPLKFPDSAVDKIMMISYMIDGEGFLITNREIVSK
DIEDFEYTPKPEYKGVFTIFNEETEKELLVRFFDHIKEEKPTVIATFNGDFFDWPFVE
ARAAFHGIDMYQEIGFQKDAEEEYKSTHCVHMDCFRWVKRDSYLPQGSQGLKAVTTAK
LGYNPIEVDPEMMTPYAIEQPQVMAEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNP
DEVLRKGTGTLCEMLLMVQAYKNGILLPHKHKDPAERFYDGHLIESETYVGGHVESLE
AGVFRSDIPTDFNIDTTAIDELLKDLDSALNFTIEVEAKKKVSDITNYDEVKADVTAA
LIKLKTDPKRHERPSIYHVDVASMYPNIMTTNRLQPDSMISEEDCASCDFNRPGKTCD
RRLPWAWRGEFFPTKKDEYLMIRNSLQNERFPGRFPDSPSRDFKDLSSTEQAAAIKKR
ITEYSKKVYHKVKVTETIEREAIICQRENPFYVNTVRDFRDRRYEFKGLQKVWKRKVD
DIPASDVSGREEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTG
ATIIQMARSLVERIGRPLELDTDGIWCILPKTFPEDFTFNLTDGKKLPISYPCVMLNH
LVHAKFTNHQYQILEDSTTLRYKTISDNSIFFEVDGPYKAMILPTSKEEDKNLKKRYA
VFNPDGSLAELKGFEVKRRGELRLIKAFQSQVFSVFLKGTTLTECYAEVGKVANSWLD
ILDSKGKTLEEDDLIDLISENRSMSKTLQEYEGMKSTSICTARRLAEFLGSQMVKDKG
LACKYIISKKPLGAAITDRAVPVAIFSAETSVKSHYLKKWLKDPGLDDYDPRSILDWD
YYRERLASTIQKMVTIPAALQQVSNPVSRIQHPDWLLKRISTKNDKLKQKKLSSFFST
GSKPMTDASNKVNQLSGSILNNDRIHDIEDLTLGDARVTTAKIGKVAMKRKGNNSEAQ
SVALQEQFVSLSLEMPSPEEDYGEWLRYHKKKWAIQKQSRQNRQHLFGDSSRKIGVSG
LIMQQTETAYSNSWQFLQFCPSEKGGEARAFVYINDKIQTIRINVGRRLYVNFRRATP
SVYELPNCKVEKVNHTLPNGRTSDHLYRLHMSESAYETEMAKADSVLKHANIEGIYES
QLGAEDRSLLELGCTTILDNSKPGLLGAGLEKGFSLEWLKPYNEVYLLNSKLNYLFLC
HLVSHELQVFALVPSWSSQAFVFVLRPSRSAQGLPNLSKSYEEFLKESNVSMDPGSPI
FNYQDQLVFEESYFDDISKLYKKLGQAITKLHSENATKAILCIQSPQAGRLKKLLRPI
NDFPNIEIRSSTMAIPQIGWQTAAAKRICKSYLSLRSWIGLYCRLSRYSDVPIGNLKG
DDIRYLIDVIYARRLQQSNIVLWWSNSPIPDNGGSEKDSILPLIDPVSLPTVNNSGVY
SKICIDLQVRNLSVNTILTAAIINEAEGADLAGSIVQGDGSSNSTPFVENAFSTPALA
VLSNLVKEWWNQALANDETADDMVNCFISWVSSSNSFLYDQTLYYHVQNLSKKAFIQL
VREFRKAGSQLVFADQKRFVLMTPKKILENTYSYANFLIKTIRSKPLFNFLDISLTEY
WEVLVWMDDVNFCGKSCKSLSASGKDTVQTFFNWHIGRFLPPLLQTEFEESVLSFMEK
YCDYRDQFQKKQQETLGVRQTQISSTALQRVSTDEDNNDSEIDNHFANGIFEAIRPQL
VKRVKQLLKIYLDGKSNPDIAVQFEFPVLAGSRLQLSNPITQFVKSLCAVYGLSKELN
LESRTLRRNLLSILGIKEFTEEASFKNPSASLIIDSVVCENCYFVCNLDLCRQDDLVR
KDTVGNTVYYSWACENCGKDYNRIVLEERLIQNIQKLVTSYQIQDLKCEKCKKIKSDE
LSVRCECSGNWVETIPSSHVLKSIETYDRVAQFYELKFLQDVTSHLI"
gene complement(<820501..>820794)
/gene="RAD14"
/locus_tag="AWJ20_3964"
/db_xref="GeneID:30036043"
mRNA complement(<820501..>820794)
/gene="RAD14"
/locus_tag="AWJ20_3964"
/product="Rad14p"
/transcript_id="XM_018881006.1"
/db_xref="GeneID:30036043"
CDS complement(820501..820794)
/gene="RAD14"
/locus_tag="AWJ20_3964"
/inference="similar to AA sequence:KEGG_Orthology:K10847"
/note="Protein that recognizes and binds damaged DNA
during NER; subunit of Nucleotide Excision Repair Factor 1
(NEF1); contains zinc finger motif; homolog of human XPA
protein; NER stands for nucleotide excision repair;
GO_component: GO:0000110 - nucleotide-excision repair
factor 1 complex [Evidence IPI] [PMID 8621533];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
9852079]; GO_function: GO:0003677 - DNA binding [Evidence
IEA]; GO_function: GO:0003684 - damaged DNA binding
[Evidence IEA]; GO_function: GO:0003684 - damaged DNA
binding [Evidence IDA] [PMID 23925126]; GO_function:
GO:0003684 - damaged DNA binding [Evidence IDA] [PMID
8516285]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0008270 - zinc ion binding
[Evidence IDA] [PMID 8516285]; GO_process: GO:0006281 -
DNA repair [Evidence IEA]; GO_process: GO:0006974 -
cellular response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0006289 - nucleotide-excision repair
[Evidence IEA]; GO_process: GO:0000715 -
nucleotide-excision repair, DNA damage recognition
[Evidence IDA] [PMID 8516285]"
/codon_start=1
/product="Rad14p"
/protein_id="XP_018733640.1"
/db_xref="GeneID:30036043"
/translation="MMLYLRYQVEEYAFKKWGSPEGLDKEYERREAAKKQRKEKKFLD
KLKDMRKKTRAEAITRHADERHEHEWSAPMDGLQGMVSRRCKVCGMTTEEIVF"
gene <822754..>823404
/gene="ORC5"
/locus_tag="AWJ20_3965"
/db_xref="GeneID:30036044"
mRNA <822754..>823404
/gene="ORC5"
/locus_tag="AWJ20_3965"
/product="origin recognition complex subunit 5"
/transcript_id="XM_018881007.1"
/db_xref="GeneID:30036044"
CDS 822754..823404
/gene="ORC5"
/locus_tag="AWJ20_3965"
/inference="similar to AA sequence:KEGG_Orthology:K02607"
/note="Subunit of the origin recognition complex (ORC);
ORC directs DNA replication by binding to replication
origins and is also involved in transcriptional silencing;
GO_component: GO:0031261 - DNA replication preinitiation
complex [Evidence IDA] [PMID 9554851]; GO_component:
GO:0005664 - nuclear origin of replication recognition
complex [Evidence IDA,IMP] [PMID 9372948]; GO_component:
GO:0005656 - nuclear pre-replicative complex [Evidence
IDA] [PMID 16824194]; GO_component: GO:0005656 - nuclear
pre-replicative complex [Evidence IDA] [PMID 9335335];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_component: GO:0000808 - origin recognition complex
[Evidence IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IMP] [PMID 9038340]; GO_function: GO:0003688 -
DNA replication origin binding [Evidence IDA] [PMID
16824194]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_process: GO:0006260 - DNA replication
[Evidence IEA,IEA]; GO_process: GO:0006270 - DNA
replication initiation [Evidence IMP] [PMID 16716188];
GO_process: GO:0006270 - DNA replication initiation
[Evidence IGI,IMP] [PMID 7579692]; GO_process: GO:0030466
- chromatin silencing at silent mating-type cassette
[Evidence IDA] [PMID 12897051]; GO_process: GO:0006267 -
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [Evidence IDA] [PMID 16824194];
GO_process: GO:0031938 - regulation of chromatin silencing
at telomere [Evidence IMP] [PMID 16980387]"
/codon_start=1
/product="origin recognition complex subunit 5"
/protein_id="XP_018733641.1"
/db_xref="GeneID:30036044"
/translation="MDTLVKMWPVFVDPILKGKYKISEFVRIYKENIYLFTGDAVVAD
RLVDLEAEDLIAMVKTRREIATQAKAYELPIQSKYVLCAAYLASYNPPRYDVRFFSKA
KDARAKRRDTGRRKSLKINPRSLAAPAFDLERMLAILHSIIPTDEEEKNVRAGSHIDI
GVQIATLTTLKLIIRTSNSDPLDSRTRWKVNASWTLVKRLADDINFPIEEFLLESE"
gene complement(<823526..>825286)
/gene="VPS27"
/locus_tag="AWJ20_3966"
/db_xref="GeneID:30036045"
mRNA complement(<823526..>825286)
/gene="VPS27"
/locus_tag="AWJ20_3966"
/product="ESCRT-0 subunit protein VPS27"
/transcript_id="XM_018881008.1"
/db_xref="GeneID:30036045"
CDS complement(823526..825286)
/gene="VPS27"
/locus_tag="AWJ20_3966"
/inference="similar to AA sequence:KEGG_Orthology:K12182"
/note="Endosomal protein that forms a complex with Hse1p;
required for recycling Golgi proteins, forming lumenal
membranes and sorting ubiquitinated proteins destined for
degradation; has Ubiquitin Interaction Motifs which bind
ubiquitin (Ubi4p); GO_component: GO:0033565 - ESCRT-0
complex [Evidence IPI] [PMID 12055639]; GO_component:
GO:0005768 - endosome [Evidence IEA]; GO_component:
GO:0005768 - endosome [Evidence IDA] [PMID 12055639];
GO_component: GO:0010008 - endosome membrane [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA,IEA]; GO_function: GO:0032266 -
phosphatidylinositol-3-phosphate binding [Evidence IDA]
[PMID 9702203]; GO_function: GO:0043130 - ubiquitin
binding [Evidence IDA] [PMID 12055639]; GO_function:
GO:0043130 - ubiquitin binding [Evidence IDA] [PMID
20150893]; GO_process: GO:0006886 - intracellular protein
transport [Evidence IEA]; GO_process: GO:0045324 - late
endosome to vacuole transport [Evidence IMP] [PMID
12055639]; GO_process: GO:0045053 - protein retention in
Golgi apparatus [Evidence IMP] [PMID 8649377]; GO_process:
GO:0006623 - protein targeting to vacuole [Evidence
IMP,IPI] [PMID 12055639]"
/codon_start=1
/product="ESCRT-0 subunit protein VPS27"
/protein_id="XP_018733642.1"
/db_xref="GeneID:30036045"
/translation="MSWFSSVVSIDERIEKATSESLPSGEQDLALNLEICDLIRSKTV
PPKDAMRSLKKRLNHKNPNVQLLTLHLIDICIKNGGSHFLTEIASREFMDSFVSVLQP
SSGLVNRDVKELMLEYLQNWANAFEGQIQLVYVNKVYEKLKKDGFHFPSGAKISSSFI
DSSAPPEWVDADECMECNTPFSFVNRKHHCRNCGGVYIQKHCNNYTTLPHYGINQPVR
VCDSCFNKLRGSSSGGPSSRVHNSNDQVRSHTTSNNRDLSSVDDDNDSDLQRALKLSL
EESQNPGLTKPSPNRAPTNHLVQNDDDDEDMKAAIAASLRDMENEGKAIPSGTDTTNP
SNNLYQLAPPSVSIADVEPKSVENTVLSIVDDEFTSVDAEIIHRYVQIVESLQRNPSA
FAIRDTKLELLHNNVLSLRPKLSKDLRRTVEKCDQLEDLHGKLTAIVRYYDKLLEDRF
SYSMKKYGHMDEGNQYTSSVVSSQMPGGQYHQYQTGSGPVTYPEGNSPYYPPVQDSDP
HYGVNTETYYQQQHDGPLPSEPPLPEGNAGYQGFYPALDERGNISQNQSIQDPDNQSS
EPNYSKPGTKEEEEPILIEL"
gene <825831..>826754
/gene="IST1"
/locus_tag="AWJ20_3967"
/db_xref="GeneID:30036046"
mRNA join(<825831..825899,826146..>826754)
/gene="IST1"
/locus_tag="AWJ20_3967"
/product="Ist1p"
/transcript_id="XM_018881009.1"
/db_xref="GeneID:30036046"
CDS join(825831..825899,826146..826754)
/gene="IST1"
/locus_tag="AWJ20_3967"
/inference="similar to AA sequence:KEGG_Orthology:K19476"
/note="Protein with positive role in the multivesicular
body sorting pathway; functions and forms a complex with
Did2p; recruitment to endosomes is mediated by the
Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with
Vps4p; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 18032584]; GO_component: GO:0005768 - endosome
[Evidence IEA,IEA]; GO_component: GO:0005768 - endosome
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005768 -
endosome [Evidence IDA] [PMID 18032584]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0032511 - late endosome to vacuole transport via
multivesicular body sorting pathway [Evidence IGI] [PMID
18032584]; GO_process: GO:0015031 - protein transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Ist1p"
/protein_id="XP_018733643.1"
/db_xref="GeneID:30036046"
/translation="MAVNRLRLIEQKETALAKQARRESRDCDPGLEEAVKTIIYSAQR
TEVKELHQIREIFVHKFGKDFAQDSIDNLSNSIPEKVTKRLSTVPPSEELVTLYLAEI
ARAYHVPFSGLQLEGEDITEHDESGDEGPPSGRVPEEALSTGGSQTARVKVRRLSQVD
KLPNPSAPKSPVSVSTPAPTTDNPHPVLRVSTSGASQKGANGKANSADEQELDALRNR
FEALRRR"
gene complement(<826881..>827792)
/gene="TAM41"
/locus_tag="AWJ20_3968"
/db_xref="GeneID:30036047"
mRNA complement(<826881..>827792)
/gene="TAM41"
/locus_tag="AWJ20_3968"
/product="Tam41p"
/transcript_id="XM_018881010.1"
/db_xref="GeneID:30036047"
CDS complement(826881..827792)
/gene="TAM41"
/locus_tag="AWJ20_3968"
/inference="similar to AA sequence:KEGG_Orthology:K17807"
/note="Mitochondrial phosphatidate cytidylyltransferase
(CDP-DAG synthase); required for cardiolipin biosynthesis;
viability of null mutant is strain-dependent; mRNA is
targeted to the bud; mutant displays defect in
mitochondrial protein import, likely due to altered
membrane lipid composition; GO_component: GO:0031314 -
extrinsic component of mitochondrial inner membrane
[Evidence IDA] [PMID 16790493]; GO_component: GO:0031314 -
extrinsic component of mitochondrial inner membrane
[Evidence IDA] [PMID 16943180]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0005759 - mitochondrial matrix [Evidence
IEA]; GO_component: GO:0005759 - mitochondrial matrix
[Evidence IDA] [PMID 16790493]; GO_component: GO:0005759 -
mitochondrial matrix [Evidence IDA] [PMID 16943180];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14690591]; GO_function: GO:0004605 -
phosphatidate cytidylyltransferase activity [Evidence
IDA,IMP] [PMID 23623749]; GO_process: GO:0032049 -
cardiolipin biosynthetic process [Evidence IMP] [PMID
19114592]; GO_process: GO:0032049 - cardiolipin
biosynthetic process [Evidence IGI] [PMID 20485265];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Tam41p"
/protein_id="XP_018733644.1"
/db_xref="GeneID:30036047"
/translation="MKQYPEHYSSLRFLGSGAVSALQDYVGSGVYFNPFVELNGLKIK
YGVVNIDTLLSDLQGWETLYLAGRLHKPVKILRDEPKVRFVNQANLISVLRTSLLLLP
EKFNEFELYKMIAGISYMGDPRMQFGENPNKVHNIVSNQFLNFRKLYSPLMDDLPNLS
ISSGESQVVSDGNQKVEAVNLIQDMSPLKRGNMVFRLPSEFRSKIYARYASKLNSPEL
AMASKSNSVNKNDPSVKSVSTDFDRQIADDKDLSLEVARAIRYTVSWPSLTQSAKGLL
TAGLVKSMKYSYEKISKYRLGKQAIIK"
gene <828540..>828628
/locus_tag="AWJ20_3969"
/db_xref="GeneID:30036048"
tRNA <828540..>828628
/locus_tag="AWJ20_3969"
/product="tRNA-Phe"
/db_xref="GeneID:30036048"
gene complement(<832165..>833886)
/gene="DCR2"
/locus_tag="AWJ20_3970"
/db_xref="GeneID:30036050"
mRNA complement(<832165..>833886)
/gene="DCR2"
/locus_tag="AWJ20_3970"
/product="Dcr2p"
/transcript_id="XM_018881012.1"
/db_xref="GeneID:30036050"
CDS complement(832165..833886)
/gene="DCR2"
/locus_tag="AWJ20_3970"
/note="Phosphoesterase; involved in downregulation of the
unfolded protein response (UPR), at least in part via
dephosphorylation of Ire1p; dosage-dependent positive
regulator of the G1/S phase transition through control of
the timing of START; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_component: GO:0005737
- cytoplasm [Evidence IEA,IEA]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA,IEA]; GO_function:
GO:0016788 - hydrolase activity, acting on ester bonds
[Evidence IMP,ISS] [PMID 15590836]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0004721 - phosphoprotein phosphatase
activity [Evidence IDA,IMP] [PMID 17673172]; GO_function:
GO:0042578 - phosphoric ester hydrolase activity [Evidence
IDA] [PMID 16990850]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0030968 - endoplasmic
reticulum unfolded protein response [Evidence IGI,IMP]
[PMID 16990850]; GO_process: GO:0006470 - protein
dephosphorylation [Evidence IDA] [PMID 16990850];
GO_process: GO:0006470 - protein dephosphorylation
[Evidence IDA,IMP] [PMID 17673172]; GO_process: GO:0007089
- traversing start control point of mitotic cell cycle
[Evidence IMP] [PMID 15590836]"
/codon_start=1
/product="Dcr2p"
/protein_id="XP_018733645.1"
/db_xref="GeneID:30036050"
/translation="MALLPRFWVRQLLKLGISIFVLSLLIVFVDFYFNFLPTSFQTRL
PLHQSTAVIVDLKVQSCLTVSGCAEPNGWYKIPKNLYLNTKWVRKGFVYVKRVEETEL
QDDHKVVLDLALAHSTEANGHGIPLYIVKDILGQATSEKEETEASRDAIRAIDDDEAA
KHGWTLRDEDSGLWVKQGKYHRSESVTGVDVLFGKDSVDPRPNWSLKPGFLFRDSGKE
AFASLTIRRGVENKPSKPVLRVNPSGKFKILQVADLHFSTGEGICRDTFPSDSAGNCL
ADPRTLDFIKLVLDQETPDFVVMTGDQIFGDTAPDAESALLKAVAPFIEREIPYAMVF
GNHDAQGSLSKEDLMSIIATLPFSLSEAGPEDVDGIGNYVLQALSPKNDHPAISFYFL
DSHSHVALPKGGSTYDHIKSNQLKFIENTSTKIKELQKDYTHIPLSMAFFHIPIPEYR
KTNVNPVVGSHREPVMGPTNDEGTRDVLAKVGVSVVSVGHDHVNDYCMFDKPDREGET
GIWLCYGGAVGEGGYAGYGGYVRRLRLFEIDTQSASIKSWKLTREQPELKLDEQTLVS
NGHVSNE"
gene complement(<834153..>834722)
/gene="FCF1"
/locus_tag="AWJ20_3971"
/db_xref="GeneID:30036051"
mRNA complement(<834153..>834722)
/gene="FCF1"
/locus_tag="AWJ20_3971"
/product="Fcf1p"
/transcript_id="XM_018881013.1"
/db_xref="GeneID:30036051"
CDS complement(834153..834722)
/gene="FCF1"
/locus_tag="AWJ20_3971"
/inference="similar to AA sequence:KEGG_Orthology:K14566"
/note="Putative PINc domain nuclease; required for early
cleavages of 35S pre-rRNA and maturation of 18S rRNA;
component of the SSU (small subunit) processome involved
in 40S ribosomal subunit biogenesis; copurifies with
Faf1p; GO_component: GO:0005730 - nucleolus [Evidence
IEA]; GO_component: GO:0005730 - nucleolus [Evidence IDA]
[PMID 16762320]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 16769905]; GO_component: GO:0005634 -
nucleus [Evidence IEA]; GO_component: GO:0032040 -
small-subunit processome [Evidence IEA]; GO_component:
GO:0032040 - small-subunit processome [Evidence IDA,IPI]
[PMID 16769905]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0000480 -
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 16762320]; GO_process: GO:0000480 - endonucleolytic
cleavage in 5'-ETS of tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
16769905]; GO_process: GO:0000447 - endonucleolytic
cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and
LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 16762320];
GO_process: GO:0000447 - endonucleolytic cleavage in ITS1
to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 16769905]; GO_process:
GO:0000472 - endonucleolytic cleavage to generate mature
5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[Evidence IMP] [PMID 16762320]; GO_process: GO:0000472 -
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 16769905]; GO_process: GO:0006364 - rRNA
processing [Evidence IEA]; GO_process: GO:0042254 -
ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Fcf1p"
/protein_id="XP_018733646.1"
/db_xref="GeneID:30036051"
/translation="MGKAKKTRKFAQVKRVITSKDQRIKKNQEQQKPVDQDKELVKEI
PQVSSALFFQYNEAIKPPYQVLVDTNFINFSIQKKLDLVRGMMDTLYARCIPIVTDCV
MAELEKLGPKYRIALRMARDPRVQRLTCSHKGTYADDCLVHRIMQHKCYIVATNDADL
KRRIRKVPGVPLMSVGAHSYVIERLPDAF"
gene <835067..>837961
/gene="PEP5"
/locus_tag="AWJ20_3972"
/db_xref="GeneID:30036052"
mRNA <835067..>837961
/gene="PEP5"
/locus_tag="AWJ20_3972"
/product="tethering complex subunit PEP5"
/transcript_id="XM_018881014.1"
/db_xref="GeneID:30036052"
CDS 835067..837961
/gene="PEP5"
/locus_tag="AWJ20_3972"
/codon_start=1
/product="tethering complex subunit PEP5"
/protein_id="XP_018733647.1"
/db_xref="GeneID:30036052"
/translation="MNVTISTKITNLQDPDVTAVAPGRDTIFLATSKGVIKEFDSSFR
LVQSFVAYSEPDWNITHLVFLEGTSLLMTVASKTNHPLTAHLWNLDKIDKQSNGPHCH
TTITVMNGSNTFPLSAFCISSDHSILGFGYADGTVILVRGDIVRDRGSRQRVVYRSNT
PVTGLEFYEGDENGSVVLMIATVSQILTVPTTGRNSGKPDIILDKKGAALGCVTMVKG
YRTQKDRLAVARDGEIAYYTPVMRGASIALDLPKKSIYSFGHYFLVTSILSADNSSVE
ASNSFSNFVGADTTRIFIIDTLNNFIAYTEQMAAGIKFIFTQWDKLHVLGSDGILYVF
KEKDIDTRLAILKTNNLYDIAIELSTKMDLERNKILRLEREYADFLYKGDQSAEALEH
YITSIELGQTSEVILKYRDSQKIDYLTRYLEALHSEGVATKQHTTLLFNSYAKLNNLE
KLTQFVEGSQNKESIAIDYETAIRICCQAGYFTLAVYLAQRSGDTDLAVQIKLRDLKD
YKGCLEYIRSLDVTDALRILIQFSRELLDTFPVETTALLVSLFTGKFSPTETELDINA
RREIQPQTFEGSSDSITAPVLQSYRALVNYMSNVGSNAPVGSDDASHVNGRSIPTYQP
PRPRLIFSSFIDHPNEFVIFLEECLESYDKFEANEKDRADLLSTLFEMYLTLSRKATD
HDTRHGWESKARSLAMTSKTHIDHNTILLLSHLASYHDGKLLAWDAREGYEVDLFRAC
IASGNVQEAIEILHKYGEHEHELFPLALNFFISSPSVLEEAGEEFDYVLKKIKEEQIM
TPLQVIQTISVNSVATIGHIRQYLVDIIDAEKQDIDRHTKLAESYRSETRSKQEQISK
ILNDPSIVQYTVCASCGSALDLPVVHFSCKHSYHQRCLANNLIGSRSIGGSEEPVCPR
CLPELENIRAIRRSHEDVADRNDLFEIALHGSDNKFKVVTDFISRGALTG"
gene complement(<838154..>840124)
/locus_tag="AWJ20_3973"
/db_xref="GeneID:30036053"
mRNA complement(<838154..>840124)
/locus_tag="AWJ20_3973"
/product="arginine--tRNA ligase"
/transcript_id="XM_018881015.1"
/db_xref="GeneID:30036053"
CDS complement(838154..840124)
/locus_tag="AWJ20_3973"
/inference="similar to AA sequence:KEGG_Orthology:K01887"
/note="Arginyl-tRNA synthetase; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; YDR341C has a paralog, MSR1, that
arose from the whole genome duplication; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0004812 -
aminoacyl-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0004814 - arginine-tRNA ligase activity
[Evidence IEA,IEA]; GO_function: GO:0004814 -
arginine-tRNA ligase activity [Evidence IDA] [PMID
9622124]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA,IEA]; GO_process: GO:0006420 -
arginyl-tRNA aminoacylation [Evidence IEA]; GO_process:
GO:0006420 - arginyl-tRNA aminoacylation [Evidence IDA]
[PMID 9622124]; GO_process: GO:0006418 - tRNA
aminoacylation for protein translation [Evidence IEA];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="arginine--tRNA ligase"
/protein_id="XP_018733648.1"
/db_xref="GeneID:30036053"
/translation="MSELHTLTERLNSLGLGSALTPFPGSDPVNNPFDLFRSYISEEL
SKITGVSRDLVYPALEWTNTPDRGDLVIPIPRLRVKGTPPIELAKKWAAEFPENSYLK
AVVANGPFIQFFFNPSVLFNLVLKDILTLNDDFGNSTIGKGKRTVVEFSSPNIAKPFH
AGHLRSTIIGGFISNLHEKLGYEVIRLNYLGDWGKQFGILAVGFKRYGSEQELESNPI
NHLFNVYVRINKDIEAEKEASTNAYYEDHPNEKPQEKVEVEEKKPQEIKKGDKKPELA
TPAVPGVPAFVPTSATDDEARSYFKKMEDGDEEALALWKKFRDLSIVKYKDTYKRLNI
EYDSYSGESQVSKEVMQKATKLFEEKNLLTEDKGAKLIDLTPYNKKLGKAIVQKKDGT
SLYLTRDVGAAMDRYEKYKFDKMIYVIASQQDLHTAQFFKILDLMGFEWASRLQHVNF
GMVQGMSTRKGTVVFLDNILEETKDAMHDVMKKNEAKYSQVENPDEIADLVGIAAVMI
QDMQAKRINNYAFNWSRMLSFEGDTGAYLQYAHSRLCSIERNAESLVDLSTLHEADFS
LLKEPIAEELVRILAQYPDILLASLRSLEPSTVVTYLFKLTHCVSSCYDILWVAGQTP
ELAKARLALYSSARRVLSNGMELLNLTPVKRM"
gene <840468..>841616
/locus_tag="AWJ20_3974"
/db_xref="GeneID:30036054"
mRNA <840468..>841616
/locus_tag="AWJ20_3974"
/product="hypothetical protein"
/transcript_id="XM_018881016.1"
/db_xref="GeneID:30036054"
CDS 840468..841616
/locus_tag="AWJ20_3974"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733649.1"
/db_xref="GeneID:30036054"
/translation="MSILEILAWIVLAKRDKCEAGGSFLDRITFALALHFYKFCTFVD
CLIHGLSRTEVLISRVREPQKTHVLKQLIWNVWAHSQLQDLQGEWSNEQELPLVGKTD
NIDSSFVGLLTDVPQSIGLVYEDLLERAANPTTWSDGKDRYIEIYNSVFQATNEAEID
IEESVILSSGLRHRRKTGRALSANKTTEEAVPAPKPHNRGNLDWVALGFQGSDPATDF
RGSGKFGEECFYRFCTVTNPKVVLRTIMLQSGSFNSGPVVLSKPWYSVALVSIHLSQF
ILTLIQRDTTRLAFYRGVLLSLSLESLNGANKLVDIEDCIYTLHSTLLTKFHKTWMEA
VAKGDITSVMDTEPFLENFKTTMTEWLWLEHSWAVDPEDTYEILEFSE"
gene complement(<841645..>843585)
/locus_tag="AWJ20_3975"
/db_xref="GeneID:30036055"
mRNA complement(<841645..>843585)
/locus_tag="AWJ20_3975"
/product="gephyrin"
/transcript_id="XM_018881017.1"
/db_xref="GeneID:30036055"
CDS complement(841645..843585)
/locus_tag="AWJ20_3975"
/inference="similar to AA sequence:KEGG_Orthology:K15376"
/codon_start=1
/product="gephyrin"
/protein_id="XP_018733650.1"
/db_xref="GeneID:30036055"
/translation="MFRVHLIIVSDTCSKDPSKDRVIPAVSEIINLRSDEYVLKGTSI
IPDEKTFIQSEISSVARDSKIDLIITSGGTGFGVRDVTPEAVAPLLDRQAPGLVQMMI
QESLKITPMAALSRPVAGVMGKCIVITLPGSPKGACENLTSIFPILSHALSLACGSSA
TPHGVSQKLQNVNSSHDVGHHKHNHPHNGACEHSHKGPKFYNRSNDLSRPVTERARQS
PYPMISVDEALDVINATVPTPRVEERNIDQTLIGYVIAENIYSTVNVPGYRASIVDGY
AVGNYSFPNTYDVSDISHANSLASSTRLESNTIVRVTTGAPVPNGTFAVIPVESTYVE
SSRVDSNGKEEEARVFIDVDEIVKEGDNIREPGSDVEINDLILSEGTEITAVGGEIGL
LSSVGLRTVNVYRKPIIGIMSTGDEVVDPSSCSSLIGGQVWDSNRPSLITACKGNNFE
VVDLGIVEDVANRLESSIRQAYSKRGVDIIITTGGVSMGELDLLKPTIERALNGIIHF
GRVAMKPGKPTTFGSFGNKLIFGLPGNPASALVTFHLFVLPCLRLWSGRSKNRYKLPT
VKVKLGEAFYLDPRPEYQRAKILQNDDGCLIAYSTGRQRSSRVGSMYTANGLLCLPSS
TNQSSPILPEGNVIEAIMIGPL"
gene <844164..>845768
/gene="PUT4"
/locus_tag="AWJ20_3976"
/db_xref="GeneID:30036056"
mRNA <844164..>845768
/gene="PUT4"
/locus_tag="AWJ20_3976"
/product="proline permease PUT4"
/transcript_id="XM_018881018.1"
/db_xref="GeneID:30036056"
CDS 844164..845768
/gene="PUT4"
/locus_tag="AWJ20_3976"
/inference="similar to AA sequence:KEGG_Orthology:K16261"
/note="Proline permease; required for high-affinity
transport of proline; also transports the toxic proline
analog azetidine-2-carboxylate (AzC); PUT4 transcription
is repressed in ammonia-grown cells; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence ISS]
[PMID 10654085]; GO_function: GO:0015193 - L-proline
transmembrane transporter activity [Evidence IGI] [PMID
14968425]; GO_function: GO:0015193 - L-proline
transmembrane transporter activity [Evidence IMP] [PMID
3552672]; GO_function: GO:0015171 - amino acid
transmembrane transporter activity [Evidence IEA];
GO_function: GO:0015175 - neutral amino acid transmembrane
transporter activity [Evidence IDA] [PMID 10654085];
GO_process: GO:0003333 - amino acid transmembrane
transport [Evidence IEA]; GO_process: GO:0006865 - amino
acid transport [Evidence IEA,IEA]; GO_process: GO:0015812
- gamma-aminobutyric acid transport [Evidence IDA,IGI]
[PMID 8455553]; GO_process: GO:0015804 - neutral amino
acid transport [Evidence IDA] [PMID 10654085]; GO_process:
GO:0015824 - proline transport [Evidence IDA] [PMID
10654085]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA,IEA]"
/codon_start=1
/product="proline permease PUT4"
/protein_id="XP_018733651.1"
/db_xref="GeneID:30036056"
/translation="MKNTENKVATFEYNVGIEEGSVEVVEVFKEEQRLEKRLEQRHIQ
MIALVGVIGTGLFLSSGSSLQKAGPLGMLLSYLTIAIIVSLNQLAMAETAALMPSTAS
SIRQAQHFIDPAMSFAYGWLQMWSIIVPSELVATAVIVSYWTDLSSGIWLTVFIVLSV
LSNCWSVRVYGELEFFFAILKILLILGLILAGLVLDLGGVPGQPRLGFHYWKDPGVFA
SFMKPGSAGKAIGFFSVLNSAVYSFGGVQNIAYLSGETKNPRRNIPKAAKRILYRVVS
FYMITLFIMSLIVPYNDPRISNGSGNASGSPFVVAFEQAGIKALPSIVNAIVLTSAWS
AANTGLVAGSRTLFSLANNGQAPKIFLRTNKRGLPWVGTIVTALFMPLSYMTLGSSSS
IVFGYLVNVTSSILLINWILISAIHYRLNRALEVQGYSRSDLPFHIRGGKALGLISGI
SSSLLLLIGGFTIFIKWDTADFVSAYVTIPLFVGFFTFWKLFKKTKWIRYEDIDLKSL
FQDVELNPEPPEEPLRGWKRLQFLWA"
gene <846185..>847399
/gene="SEN2"
/locus_tag="AWJ20_3977"
/db_xref="GeneID:30036057"
mRNA <846185..>847399
/gene="SEN2"
/locus_tag="AWJ20_3977"
/product="Sen2p"
/transcript_id="XM_018881019.1"
/db_xref="GeneID:30036057"
CDS 846185..847399
/gene="SEN2"
/locus_tag="AWJ20_3977"
/inference="similar to AA sequence:KEGG_Orthology:K15322"
/note="Subunit of the tRNA splicing endonuclease; tRNA
splicing endonuclease is composed of Sen2p, Sen15p,
Sen34p, and Sen54p; Sen2p contains the active site for
tRNA 5' splice site cleavage and has similarity to Sen34p
and to Archaeal tRNA splicing endonuclease; GO_component:
GO:0012505 - endomembrane system [Evidence IEA];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005741 - mitochondrial outer membrane
[Evidence IEA,IEA]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA,IMP] [PMID
12925762]; GO_component: GO:0005741 - mitochondrial outer
membrane [Evidence IDA] [PMID 16407407]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0000214 - tRNA-intron endonuclease
complex [Evidence IEA]; GO_component: GO:0000214 -
tRNA-intron endonuclease complex [Evidence IDA] [PMID
9200603]; GO_function: GO:0016829 - lyase activity
[Evidence IEA]; GO_function: GO:0004518 - nuclease
activity [Evidence IEA]; GO_function: GO:0003676 - nucleic
acid binding [Evidence IEA]; GO_function: GO:0000213 -
tRNA-intron endonuclease activity [Evidence IEA];
GO_function: GO:0000213 - tRNA-intron endonuclease
activity [Evidence IMP] [PMID 2182322]; GO_function:
GO:0000213 - tRNA-intron endonuclease activity [Evidence
IDA] [PMID 9200603]; GO_process: GO:0090501 - RNA
phosphodiester bond hydrolysis [Evidence IEA]; GO_process:
GO:0090502 - RNA phosphodiester bond hydrolysis,
endonucleolytic [Evidence IEA]; GO_process: GO:0090305 -
nucleic acid phosphodiester bond hydrolysis [Evidence
IEA]; GO_process: GO:0008033 - tRNA processing [Evidence
IEA]; GO_process: GO:0006388 - tRNA splicing, via
endonucleolytic cleavage and ligation [Evidence IEA];
GO_process: GO:0000379 - tRNA-type intron splice site
recognition and cleavage [Evidence IMP] [PMID 2182322];
GO_process: GO:0000379 - tRNA-type intron splice site
recognition and cleavage [Evidence IDA] [PMID 9200603]"
/codon_start=1
/product="Sen2p"
/protein_id="XP_018733652.1"
/db_xref="GeneID:30036057"
/translation="MKKSLNQLYGSPLPLVVRPLPPIIPHNPLSWIFWLWEYWFPEPE
YNLYDGILHALVLPSETTFYVRIDNGSDINSLWSMGFFGKGTLSRSEPSWYTRTARRL
GLQGGEQMTSEEVTAARREERKKFKMMRAQAEAEELARRKKLDEMKEVWAGQDSDSVS
TLVDEESKLTSTGVASEAGDSKHELTRTLEESQPAQAAAVLVESSASPEVSGNHATVI
RFEDQSIIDSNNKLIQQEYLQLMPQEAFFLSYGLGVLRITDHVTGKPLTSEELLTRFV
SANPRFLSQYAVYHHYRSLGWCVRSGVKFGSELLLYKRGPPFSHAEFCIRILTSPGDE
QHDWWWNTSIGRVVGGVKKGLVFCYVQGNDTENTAKILDASEGYSIKDILGSYQIREV
VYRRWIATRNRD"
gene <850123..>850623
/locus_tag="AWJ20_3978"
/db_xref="GeneID:30036058"
mRNA <850123..>850623
/locus_tag="AWJ20_3978"
/product="hypothetical protein"
/transcript_id="XM_018881020.1"
/db_xref="GeneID:30036058"
CDS 850123..850623
/locus_tag="AWJ20_3978"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733653.1"
/db_xref="GeneID:30036058"
/translation="MNKKRATVYFVSTLAVFAGIEAGCIIPFTRLYEKGIEWPTTLVG
IIAAVLLGVGLLPPYWEIYKRGGQVVGINFIFLTIDSGGALFSTLSLVFQKGSLDILG
CVLYIVILVLEGGIFLSQAIWLLRTRIVRRAASEERVCCDEVGSGLKGDKLVLSEAIP
DENEIV"
gene complement(<850722..>851177)
/locus_tag="AWJ20_3979"
/db_xref="GeneID:30036059"
mRNA complement(<850722..>851177)
/locus_tag="AWJ20_3979"
/product="hypothetical protein"
/transcript_id="XM_018881021.1"
/db_xref="GeneID:30036059"
CDS complement(850722..851177)
/locus_tag="AWJ20_3979"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733654.1"
/db_xref="GeneID:30036059"
/translation="MSPSVLARVISGKLELDVKSLKVNPAKLENFKRHKVKDVDYPAV
IRGDDKDLVLGTVVYNLSEEEIIKLDMFEGEEYERLVLDVLDIAESRQVKAGVYVWVD
SQDRLLEEEWDFGYFVNHKMENWISDELKEMDKEAEARGGRFWDTTQCI"
gene complement(<852101..>852478)
/locus_tag="AWJ20_3980"
/db_xref="GeneID:30036061"
mRNA complement(<852101..>852478)
/locus_tag="AWJ20_3980"
/product="splicing factor, SYF2 family"
/transcript_id="XM_018881023.1"
/db_xref="GeneID:30036061"
CDS complement(852101..852478)
/locus_tag="AWJ20_3980"
/codon_start=1
/product="splicing factor, SYF2 family"
/protein_id="XP_018733655.1"
/db_xref="GeneID:30036061"
/translation="MSKSSSSPDLKLSREERFKSLRKRANESLKANRSEVYEEHKRSK
VDEKLQARLQRQREAAEFELAELESKETGEDFERKRVWDWTIEESEKWDQKKNKVEKS
IESSVFSGKFAISRIVLVQILGS"
gene <855167..>856948
/locus_tag="AWJ20_3981"
/db_xref="GeneID:30036062"
mRNA <855167..>856948
/locus_tag="AWJ20_3981"
/product="hypothetical protein"
/transcript_id="XM_018881024.1"
/db_xref="GeneID:30036062"
CDS 855167..856948
/locus_tag="AWJ20_3981"
/inference="similar to AA sequence:KEGG_Orthology:K12864"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733656.1"
/db_xref="GeneID:30036062"
/translation="MSSTDQIFKVSIGILRALKRVDLGSGANSRQVASGPAPSDESTK
TDGFEDDPRFYGNDLESSLQGQVLDYIELKEKAIGQLAGADSFHNRSLLKKVVARFVG
ARETNETLRVKHPDEPLQFLESEEELFERLKDLTILADVGLYREMSEMDDLKDGLLSL
LFHENSDIVVQLFVVLLELLQPDSETDVSSIHNFYRILINGGLLKGIEQYLIRLQKPN
QEAQEDDVLGRRVEEEKATLDLLSSLAEIDEFILNFSNCSDLFDSLITRIQNKEPGDL
VSENRLSCALIVQSLLSNGPLAKVLITASSVESILNVTSTYIYKDPEPYETDKLFSEC
VFDVLSSISVNSRDDFLNFEGPELMIKLLRDANWAVGPALRVLKTSVRDDDGRIANLI
IESGGLKHLFKLLYKGSKGEVDDVISVLANLLHWLPLESPGRIRVIGKLIEKDYRAVR
LLLRKRRKFCNLIGELRSRLSDSALEEKMEIITELEEAGVDKQNRIDIILAWLTVEST
RIGRDVIKLLSSEPGNLFQSLRSYISELNLIIRAETEEPAMPKDELEFLRMEEEMVSS
LVSQMKHSLQPGYVNLQQGSYKKIISQ"
gene <857672..>860077
/gene="SNF5"
/locus_tag="AWJ20_3982"
/db_xref="GeneID:30036063"
mRNA <857672..>860077
/gene="SNF5"
/locus_tag="AWJ20_3982"
/product="Snf5p"
/transcript_id="XM_018881025.1"
/db_xref="GeneID:30036063"
CDS 857672..860077
/gene="SNF5"
/locus_tag="AWJ20_3982"
/inference="similar to AA sequence:KEGG_Orthology:K11648"
/note="Subunit of the SWI/SNF chromatin remodeling
complex; involved in transcriptional regulation; functions
interdependently in transcriptional activation with Snf2p
and Snf6p; relocates to the cytosol under hypoxic
conditions; GO_component: GO:0016514 - SWI/SNF complex
[Evidence IDA] [PMID 18644858]; GO_component: GO:0016514 -
SWI/SNF complex [Evidence IDA] [PMID 8016655];
GO_component: GO:0016514 - SWI/SNF complex [Evidence IDA]
[PMID 8127913]; GO_component: GO:0016514 - SWI/SNF complex
[Evidence IDA,IMP] [PMID 8159677]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 22932476];
GO_component: GO:0000228 - nuclear chromosome [Evidence
IEA]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 2233708]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22932476]; GO_function: GO:0015616 -
DNA translocase activity [Evidence IDA] [PMID 17188033];
GO_function: GO:0001102 - RNA polymerase II activating
transcription factor binding [Evidence IPI] [PMID
11865042]; GO_function: GO:0001102 - RNA polymerase II
activating transcription factor binding [Evidence IMP,IPI]
[PMID 14580348]; GO_process: GO:0000436 - carbon
catabolite activation of transcription from RNA polymerase
II promoter [Evidence IGI] [PMID 14580348]; GO_process:
GO:0006338 - chromatin remodeling [Evidence IEA];
GO_process: GO:0006338 - chromatin remodeling [Evidence
IGI,IMP] [PMID 1459453]; GO_process: GO:0000724 -
double-strand break repair via homologous recombination
[Evidence IMP] [PMID 16024655]; GO_process: GO:0045944 -
positive regulation of transcription from RNA polymerase
II promoter [Evidence IMP] [PMID 1339306]; GO_process:
GO:0045944 - positive regulation of transcription from RNA
polymerase II promoter [Evidence IGI] [PMID 1901413];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 3542227]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0005987 - sucrose catabolic process
[Evidence IMP] [PMID 6392017]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Snf5p"
/protein_id="XP_018733657.1"
/db_xref="GeneID:30036063"
/translation="MSFPFEQQYNQFMVDESSGTQFNSDNGGSANMLISQVPGNTSNQ
QLMQQSSQPSQQQQFRNSFQFQGQPGSQMAGPRQNQGPAAVEQTRNVSMNQGQFHVNT
PQSQLQPQSSQGPVNQAKYAQFLQQQLQHQQHQTPALAGQADGLQTPPSAMVRGSQTN
QNAMVSGANPGNVQFQMSQYAPNTSGPQNQRINLGQSGSLGTKFAGSSALPGNPGDSA
PGGPVLSASGGPNAAGVHGIHPVPSNIGAPVVPITGHQPSDRSNIPPRPALPNEILSA
VKELPNSTLEEKTLIELLTKDAEQELIFAKQEQRHAGLIYRKQVEYDYYDQVRQLRQV
QPSAIFGDGYSGYGNSWTGSRMNLIYPRDRKRGKRESSELFLSMEQLEYIASIPELLA
PIRLDIDLDKYRLRDTFTWNLNEKLISVDLFAQNLAEDYNIPLSYAPLIANNINEVLN
DFHPHAFVEAAEDQDQASSGLHSSSATGEESSGTVITSVNTSTTSSNPAGDVTVTTTT
NDGISENLTPNGYATNPTLSNRNDDMRITIKLDITVGQHNLVDQFEWDLNCMDNNPEQ
FAEAMCRDLSLSGEFATAIAHAIREQTQLFTKTLFLVGHQFDGRPIEDDDIVRELCPP
VTSDAFLRNTSFIKDFSPVLFEIPHVELDRQDKDRDRDSRRKRRQGRAGRRGGPTLPD
LRETVRTFRTPVYSSLLPGGIDKVLELVRKQATKAEESDDDDDTPRPVTVGRRGRPPA
HAQLSGVSTFRASPAALPSSSSRMASPQFRYHQQIQSVEDHYLVKLRIPPRIRDEVNR
ISL"
gene complement(<860200..>861897)
/gene="HTS1"
/locus_tag="AWJ20_3983"
/db_xref="GeneID:30036064"
mRNA complement(<860200..>861897)
/gene="HTS1"
/locus_tag="AWJ20_3983"
/product="histidine--tRNA ligase"
/transcript_id="XM_018881026.1"
/db_xref="GeneID:30036064"
CDS complement(860200..861897)
/gene="HTS1"
/locus_tag="AWJ20_3983"
/inference="similar to AA sequence:KEGG_Orthology:K01892"
/note="Cytoplasmic and mitochondrial histidine tRNA
synthetase; efficient mitochondrial localization requires
both a presequence and an amino-terminal sequence;
mutations in human ortholog HARS2 are associated with
Perrault syndrome; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IMP] [PMID 3521891]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IMP]
[PMID 3521891]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0004812 -
aminoacyl-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
[Evidence IDA] [PMID 16741232]; GO_function: GO:0004821 -
histidine-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0003729 - mRNA binding [Evidence IDA]
[PMID 23222640]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA,IEA]; GO_process: GO:0006427 -
histidyl-tRNA aminoacylation [Evidence IEA]; GO_process:
GO:0006427 - histidyl-tRNA aminoacylation [Evidence IMP]
[PMID 1459448]; GO_process: GO:0006427 - histidyl-tRNA
aminoacylation [Evidence IDA] [PMID 16741232]; GO_process:
GO:0032543 - mitochondrial translation [Evidence IGI]
[PMID 16777356]; GO_process: GO:0006418 - tRNA
aminoacylation for protein translation [Evidence IEA];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="histidine--tRNA ligase"
/protein_id="XP_018733658.1"
/db_xref="GeneID:30036064"
/translation="MRSFNRINSIRPYFESGLKSSITSASKVLAAYQMSTESTAPVET
AVPAKVRAPKAPAAPQFTLKTPKGTKDWADKDMTIRSAIFETITKVFKRHGGVTIDTP
VFELREILSGKYGEDSKLIYNLEDQGGELTSLRYDLTVPFARFVAMNGIQNIKRYHIA
KVYRRDQPAMTKGRMREFYQCDFDIAGSYENMVADSEILSIAAEGLTALGIEDFTIKV
NHRKILDGIFQHCGVKEQDVRKVSSAVDKLDKAPWSEVKKEMVVEKGQPEEVADRIGE
YVLIKGTISDVINKLKADSALSSNASAAQGIADMETALPYLEAFGVTKHISFDLSLAR
GLDYYTGLIYEAVTAASAPPEPSSKEAQSSNANKKSKKAKKLDDDDDNSENVGVGSIL
AGGRYDNLVGMFAGTSKKNASIPCVGVSFGVERLFSLIKARPGLYDNARSTSTQVFLM
AFGGGPTWTGFLNERLSVAKLLWDGGVEAEFMYKTKPKTPKQFEAAEKSGCPFAVILG
QEEYPKGIVKVKELGMGSDADQGTDVPLEQLVDFVKAKIAARNNGIDSALQLLRLDN"
gene <862339..>863472
/gene="TFA1"
/locus_tag="AWJ20_3984"
/db_xref="GeneID:30036065"
mRNA <862339..>863472
/gene="TFA1"
/locus_tag="AWJ20_3984"
/product="Tfa1p"
/transcript_id="XM_018881027.1"
/db_xref="GeneID:30036065"
CDS 862339..863472
/gene="TFA1"
/locus_tag="AWJ20_3984"
/inference="similar to AA sequence:KEGG_Orthology:K03136"
/note="TFIIE large subunit; involved in recruitment of RNA
polymerase II to the promoter, activation of TFIIH, and
promoter opening; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005673 -
transcription factor TFIIE complex [Evidence IDA] [PMID
1429681]; GO_component: GO:0005673 - transcription factor
TFIIE complex [Evidence IDA] [PMID 7961670]; GO_function:
GO:0000993 - RNA polymerase II core binding [Evidence IPI]
[PMID 11779853]; GO_function: GO:0000993 - RNA polymerase
II core binding [Evidence IDA] [PMID 8702741];
GO_function: GO:0001097 - TFIIH-class transcription factor
binding [Evidence IDA] [PMID 8702741]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0043565 - sequence-specific DNA binding
[Evidence IEA]; GO_function: GO:0003697 - single-stranded
DNA binding [Evidence IDA] [PMID 9271406]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006366 - transcription
from RNA polymerase II promoter [Evidence IDA] [PMID
1331084]; GO_process: GO:0006366 - transcription from RNA
polymerase II promoter [Evidence IDA] [PMID 1429681];
GO_process: GO:0006366 - transcription from RNA polymerase
II promoter [Evidence IDA] [PMID 7961670]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IMP] [PMID 9188494]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 9271406]; GO_process: GO:0006367 -
transcription initiation from RNA polymerase II promoter
[Evidence IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0001113 -
transcriptional open complex formation at RNA polymerase
II promoter [Evidence IDA,IGI] [PMID 10973956]"
/codon_start=1
/product="Tfa1p"
/protein_id="XP_018733659.1"
/db_xref="GeneID:30036065"
/translation="MDALLIHGVLTDDELWHMLGINKKELRALCAKLKENHLLTDHVQ
REEGPAQRPITKTYYYIHYTETIDAIKWKMHSIVKKLKEEVGAESQPQGYVCDTCKSR
YATIDVIGNFNSDRGGFVCDVCDSLLREDDSSAESQAQQEKLGRLMSQIDPIIDALRQ
IDEVHIPENTFQSSLAHALPAPSSTGPTTATTTSSAIKFAKSANQGGVNGTTSLQVNI
TSDKESAEIERKNREERARLAEENALPTWHLESTVGKSLYDSTNSSATSGDKMDANAP
ILSDRSTIKLEDSDKPATSADNIVSSVNSTTNSVAEDKASEEALASYYAQLAARQEAS
DDEDEDDEEDEDEDEDDDAEFEDVITADVKSTIDDEDDDDDED"
gene complement(<864284..>866941)
/locus_tag="AWJ20_3985"
/db_xref="GeneID:30036066"
mRNA complement(<864284..>866941)
/locus_tag="AWJ20_3985"
/product="hypothetical protein"
/transcript_id="XM_018881028.1"
/db_xref="GeneID:30036066"
CDS complement(864284..866941)
/locus_tag="AWJ20_3985"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733660.1"
/db_xref="GeneID:30036066"
/translation="MTTRSNKKRQKEDMKFSIGYSKDVYENPTGSASVVGDTSHNYEE
ETVEESDEDVDMQDMQDNSNSPRNNRNGNCRNIDPTMAVLPPNSKGIEGVFPEIINSF
VGFPVTYATNGPAPYTSESSLAVITTPKFTECQLNIIEIRRLAHTQNLLLKTKKVSEP
GNTHVAQRVKGHGILTFNEACIYYYDHTSREAIANCPYYISKATGETYPESIIFCSPV
TTSRYIIGSESGSLYYVDISFHENKSTVEDSHSSNGVKLDIEISCFPLAIISGANNVI
HLGGSYFMVTSHVNAPMLIELDVTRLNEGLFEEFVEGSTIVPDFVTPYRVIYQLDNLG
PILDFYVQNDVSTSANQIIACSAGGTIKRITCGNLPSGVSEFVPVDHIQNIWSGVDAH
GQLLIVVGCIGETRIFVLDENDPFFVKSELLEYNGLDLNTETLYFGTMETGTTVFLTE
SNIVVFPRRGVEILNPISLTPSNDGSKLVKAEIWKDMIVVTTRNTLLLFRYRADTLSL
EQISDISLVTVGHEISDLSFVSGYILVSFWHCTELHLYRVSDKKNKIIHVRTSDLADP
SCLLSKVALKSPGIRTVLLRGSMSHEDEIMDKLKAYVGLSDGKIIVFEDLTLSKGKDY
ILGRFPVGLVAHNELLFAISDTLSVSIDTDYNFKSIGGIEASPRTVLKVSEDEGSFAV
ALGKGFQLIQLNDLQDVQIDEIDLSIKSPTQPLPLSVKPIDDHLALVLWENRILETVD
TKKMKSVGEFCLLFNEEFSSLGIVEKDGFKYCYIGGEREAKKPLFSTIDWENYNVSRS
VATKGNDALKFGVVRRYMLSADGMPVPDSLQTVQTNGAVYSMFTDGDVALLGVQSSVS
KNRMNGISSASSNSLADYPSTIQKQRDLS"
gene complement(<868717..>869025)
/gene="VMA3"
/locus_tag="AWJ20_3986"
/db_xref="GeneID:30036067"
mRNA complement(<868717..>869025)
/gene="VMA3"
/locus_tag="AWJ20_3986"
/product="H(+)-transporting V0 sector ATPase subunit c"
/transcript_id="XM_018881029.1"
/db_xref="GeneID:30036067"
CDS complement(868717..869025)
/gene="VMA3"
/locus_tag="AWJ20_3986"
/inference="similar to AA sequence:KEGG_Orthology:K02155"
/note="Proteolipid subunit c of the V0 domain of vacuolar
H(+)-ATPase; dicyclohexylcarbodiimide binding subunit;
required for vacuolar acidification and important for
copper and iron metal ion homeostasis; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016021 - integral component of membrane [Evidence IDA]
[PMID 15252052]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0033179 -
proton-transporting V-type ATPase, V0 domain [Evidence
IEA]; GO_component: GO:0033177 - proton-transporting
two-sector ATPase complex, proton-transporting domain
[Evidence IEA]; GO_component: GO:0005774 - vacuolar
membrane [Evidence IEA]; GO_component: GO:0000220 -
vacuolar proton-transporting V-type ATPase, V0 domain
[Evidence TAS] [PMID 10224039]; GO_component: GO:0000220 -
vacuolar proton-transporting V-type ATPase, V0 domain
[Evidence TAS] [PMID 9442887]; GO_component: GO:0005773 -
vacuole [Evidence IEA]; GO_function: GO:0015078 - hydrogen
ion transmembrane transporter activity [Evidence IEA];
GO_function: GO:0015078 - hydrogen ion transmembrane
transporter activity [Evidence TAS] [PMID 12482875];
GO_process: GO:0015991 - ATP hydrolysis coupled proton
transport [Evidence IEA]; GO_process: GO:0006878 -
cellular copper ion homeostasis [Evidence IMP] [PMID
9434348]; GO_process: GO:0006879 - cellular iron ion
homeostasis [Evidence IMP] [PMID 9434348]; GO_process:
GO:0006897 - endocytosis [Evidence IMP] [PMID 2145283];
GO_process: GO:0006811 - ion transport [Evidence IEA];
GO_process: GO:0006623 - protein targeting to vacuole
[Evidence IMP] [PMID 2145283]; GO_process: GO:0015992 -
proton transport [Evidence IEA]; GO_process: GO:0015992 -
proton transport [Evidence TAS] [PMID 12482875];
GO_process: GO:0006810 - transport [Evidence IEA];
GO_process: GO:0007035 - vacuolar acidification [Evidence
TAS] [PMID 10224039]; GO_process: GO:0007035 - vacuolar
acidification [Evidence TAS] [PMID 9442887]; GO_process:
GO:0007033 - vacuole organization [Evidence IMP] [PMID
2145283]"
/codon_start=1
/product="H(+)-transporting V0 sector ATPase subunit c"
/protein_id="XP_018733661.1"
/db_xref="GeneID:30036067"
/translation="MAGIIAIYGLVVSVLVTGSLKQQQALYTGFIQLGAGLSVGLAGL
AAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQDVTC
"
gene complement(<870547..>871992)
/gene="PRP46"
/locus_tag="AWJ20_3987"
/db_xref="GeneID:30036068"
mRNA complement(<870547..>871992)
/gene="PRP46"
/locus_tag="AWJ20_3987"
/product="Prp46p"
/transcript_id="XM_018881030.1"
/db_xref="GeneID:30036068"
CDS complement(870547..871992)
/gene="PRP46"
/locus_tag="AWJ20_3987"
/inference="similar to AA sequence:KEGG_Orthology:K12862"
/note="Member of the NineTeen Complex (NTC); this complex
contains Prp19p and stabilizes U6 snRNA in catalytic forms
of the spliceosome containing U2, U5, and U6 snRNAs;
GO_component: GO:0000974 - Prp19 complex [Evidence IPI]
[PMID 12088152]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005681 -
spliceosomal complex [Evidence IEA]; GO_component:
GO:0005681 - spliceosomal complex [Evidence IDA] [PMID
11884590]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0008380 - RNA splicing
[Evidence IEA]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0006397 - mRNA processing
[Evidence IEA]; GO_process: GO:0000398 - mRNA splicing,
via spliceosome [Evidence IMP] [PMID 12554883]"
/codon_start=1
/product="Prp46p"
/protein_id="XP_018733662.1"
/db_xref="GeneID:30036068"
/translation="MSSEYIGRLTSQLFPDSPLESVLDVEYTGVPRLRIRRKLVDYNE
VKDLPQELADKLAQANAKKGKSTNGGRIKKRIQREKVNLGQDADVTTAKLIETVSEND
RNSAAKSGTGSSDLAVVVQTKTPLQKQELPSINRNNFSAESAVSVRSPLHAVHAQVRP
TWHPPWKLKRVLAGHQGWVRSVCVDPDNKFFATGSADRTIKIWDLASGRLRVTLTGHI
MAVRGLVISPRHPYLFSAGEDKMVKCWDLEHNKVVRQYHGHLSSVYSVDLHPTLDLLV
TAGRDSVARVWDIRTRHPVHVLTGHKATVSQVRCQSADPQVITTSMDSTVRLWDLAAG
KTQTVLTHHKKSVRALTLHPTEFTFATGSPDNIKQWKFPEGAFMHNFAPPQNAIINTL
SVNEDGVMFAGGDDGSMGFFDWSSGYKFQQFDTVAVPGSLSAESGIFASTFDRSGLRL
ITCEADKSVKIWAEDNEATEETHPLQWKPTL"
gene <884483..>885475
/gene="NUS1"
/locus_tag="AWJ20_3988"
/db_xref="GeneID:30036069"
mRNA <884483..>885475
/gene="NUS1"
/locus_tag="AWJ20_3988"
/product="Nus1p"
/transcript_id="XM_018881031.1"
/db_xref="GeneID:30036069"
CDS 884483..885475
/gene="NUS1"
/locus_tag="AWJ20_3988"
/inference="similar to AA sequence:KEGG_Orthology:K19177"
/note="Putative prenyltransferase; required for cell
viability; proposed to be involved in protein trafficking
because tet-repressible mutant shows accumulation of
hypoglycosylated forms of CPY; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IDA] [PMID
11086160]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0005811 - lipid particle [Evidence
IEA,IEA]; GO_component: GO:0005811 - lipid particle
[Evidence IDA] [PMID 10515935]; GO_component: GO:0005811 -
lipid particle [Evidence IDA] [PMID 14690591];
GO_component: GO:0005811 - lipid particle [Evidence IDA]
[PMID 24390141]; GO_component: GO:0005811 - lipid particle
[Evidence IDA] [PMID 24868093]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005635 -
nuclear envelope [Evidence IDA] [PMID 14690591];
GO_component: GO:0031965 - nuclear membrane [Evidence
IEA]; GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_function: GO:0008834 -
di-trans,poly-cis-decaprenylcistransferase activity
[Evidence IEA]; GO_function: GO:0004659 -
prenyltransferase activity [Evidence ISS] [PMID 14690591];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_function: GO:0016765 - transferase activity,
transferring alkyl or aryl (other than methyl) groups
[Evidence IEA]; GO_process: GO:0006486 - protein
glycosylation [Evidence IMP] [PMID 16943325]"
/codon_start=1
/product="Nus1p"
/protein_id="XP_018733663.1"
/db_xref="GeneID:30036069"
/translation="MSDSGDIGAFASAVRQTPETYRSRGQPRRVSNAIARAMVSSGAT
KDSRLEPDAPLSSHLTYLVFRFILQIFYFCFSGYQWVLYYYHRLRLRISSVAVYHNRT
PQLIRSDVVSLEKIPGHVATVLNLKNESEEGGGIDGLLHEVGELAAWCVGAGISVLTV
YEKRGILKSMPIQDVNRAITRKLENYFGISNTPKFRLVIPHLHSQSSIRPGDNGDDAT
DEKFDLVINLISLTDGRTSIVELSKTLADMAMEHKLSSRDLSLSLIDRHLRDILEEPD
LVILFSPDIDLQGFPPWQIRLSEIFYLPDNDEVSYVVFLKALQKYAKCKINVGR"
gene <885790..>888651
/gene="NPR3"
/locus_tag="AWJ20_3989"
/db_xref="GeneID:30036070"
mRNA <885790..>888651
/gene="NPR3"
/locus_tag="AWJ20_3989"
/product="Npr3p"
/transcript_id="XM_018881032.1"
/db_xref="GeneID:30036070"
CDS 885790..888651
/gene="NPR3"
/locus_tag="AWJ20_3989"
/note="Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p
complexes; Npr2/3 complex mediates downregulation of TORC1
activity upon amino acid limitation; SEA complex is a
coatomer-related complex that associates dynamically with
the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required
for non-nitrogen-starvation (NNS)-induced autophagy;
required for Npr2p phosphorylation and Iml1p-Npr2p
interaction; null mutant shows delayed meiotic DNA
replication and double-strand break repair; GO_component:
GO:1990130 - Iml1 complex [Evidence IDA] [PMID 21900499];
GO_component: GO:0035859 - Seh1-associated complex
[Evidence IDA] [PMID 21454883]; GO_component: GO:0097042 -
extrinsic component of fungal-type vacuolar membrane
[Evidence IDA] [PMID 21454883]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005774 -
vacuolar membrane [Evidence IEA]; GO_component: GO:0005773
- vacuole [Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0034198 -
cellular response to amino acid starvation [Evidence IMP]
[PMID 19521502]; GO_process: GO:0007126 - meiotic nuclear
division [Evidence IEA]; GO_process: GO:0007126 - meiotic
nuclear division [Evidence IMP] [PMID 18069899];
GO_process: GO:0032007 - negative regulation of TOR
signaling [Evidence IMP] [PMID 19521502]; GO_process:
GO:0015031 - protein transport [Evidence IEA]; GO_process:
GO:0007124 - pseudohyphal growth [Evidence IMP] [PMID
24603354]; GO_process: GO:2000785 - regulation of
autophagic vacuole assembly [Evidence IMP] [PMID
21900499]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Npr3p"
/protein_id="XP_018733664.1"
/db_xref="GeneID:30036070"
/translation="MSYLNLPNPCLSAVLLTVSTHSGPQFVFNYPPVPTTIDTSLLKD
RETELLSDSESDSEWSYSSDDSNDGSDSLANEEVIQESDGGSGDSNGRSVNGTTPEPQ
YRGMPGSLLERLERPRGSTIRRHHRNLSVSQRRRSSSSVGTAVGSLKGLQKKKAHKRG
LGLSSTGNGRDTSARANGTAPHTNGNTTKNNTVTVNSKSGSGEGNIQDAEDLDITKVV
GFDTDFLGELLCPQRAMCNMRFEMCVDDTVFLGLPIHVQADGQWRKKKFSKAPRSVPS
TLMENGGINGLKARKRKTGARRARLGADGDRRLNLSGDEHGNIESTGPNETNVLSDEN
ESDASDISDVSDVGSDVSDSDGDGDGDDGTKVAQEQGEIRPGGSHPNRKADNLSASIS
SLPEFSDPQSPMRMFQVTFVMTPPVTEYKFRVDQMYYFVLANFVKTLRTEQAKSNYVW
NEVNTMLKIRDKALSTVDGNVDDGNNNGETKKKRLSPYELCDEMIEKSSLALAIAQLF
NAIDISDIANLRINNKLRSFQIPIEYEFTRLPAITEPYLSRSYLTSISDFDNIINEGV
AVGSGSSQFNVYCTILLLDSPDRIIKDIGVDPYSPLANFIRDINPTVTLQTLANTSGV
EVGHVIELMRSLIYWRKARPIAPLHHRNIYAVSPLASIGQIYKYIPLFKEEFPALPSL
PRMLSMLSTGKPRSYAFHIPSRDHRDVYLNALGWLFKYGFVMQLRTFVWLRVTKKIKM
AVNRDIQNEEQLRESIREAESTNNVQDENGGDLKNTDTSTDNTSKRDPDNIPSTNNTT
DNKATTSNTAHATTTTLISEVGGIKITSTTTPSASSNHLNELNTTTDAAGSTAGGKFS
SSSSRSDVSDMSAAAEDWKQDTILLDPTTATLLQKKWINKITESKPAEVVALFNRVVK
YFNGHTPMESVLLVENFPRQDFRKVLSTFDEFLIVVRHW"
gene complement(<888921..>889427)
/gene="UBC8"
/locus_tag="AWJ20_3990"
/db_xref="GeneID:30036072"
mRNA complement(<888921..>889427)
/gene="UBC8"
/locus_tag="AWJ20_3990"
/product="E2 ubiquitin-conjugating protein UBC8"
/transcript_id="XM_018881034.1"
/db_xref="GeneID:30036072"
CDS complement(888921..889427)
/gene="UBC8"
/locus_tag="AWJ20_3990"
/inference="similar to AA sequence:KEGG_Orthology:K10576"
/note="Ubiquitin-conjugating enzyme that regulates
gluconeogenesis; negatively regulates gluconeogenesis by
mediating the glucose-induced ubiquitination of
fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme
that catalyzes the ubiquitination of histones in vitro;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
10811607]; GO_function: GO:0005524 - ATP binding [Evidence
IEA]; GO_function: GO:0016881 - acid-amino acid ligase
activity [Evidence IEA]; GO_function: GO:0016874 - ligase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0004842
- ubiquitin-protein transferase activity [Evidence IEA];
GO_function: GO:0004842 - ubiquitin-protein transferase
activity [Evidence IDA] [PMID 1869573]; GO_process:
GO:0045721 - negative regulation of gluconeogenesis
[Evidence IMP] [PMID 12686616]; GO_process: GO:0043161 -
proteasome-mediated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 12686616]; GO_process:
GO:0016567 - protein ubiquitination [Evidence IEA];
GO_process: GO:0042787 - protein ubiquitination involved
in ubiquitin-dependent protein catabolic process [Evidence
IMP] [PMID 10811607]"
/codon_start=1
/product="E2 ubiquitin-conjugating protein UBC8"
/protein_id="XP_018733665.1"
/db_xref="GeneID:30036072"
/translation="MSDYEVTLVNDNMQEFFVKFKGPSETPFDGGAWKIHVELPDQYP
YKSPSIGFVNKIFHPNIDELSGSVCLDVINQTWSPMFDMVNIFEVFLPQLLRYPNPTD
PLNGEASALLLREPKAYEKRVQEYVKLYASGEALNNTDDNSDSDMEMSSVGSFDSDDD
EAAGQMEV"
gene <890923..>892407
/gene="SPO7"
/locus_tag="AWJ20_3991"
/db_xref="GeneID:30036073"
mRNA <890923..>892407
/gene="SPO7"
/locus_tag="AWJ20_3991"
/product="Nem1-Spo7 phosphatase regulatory subunit SPO7"
/transcript_id="XM_018881035.1"
/db_xref="GeneID:30036073"
CDS 890923..892407
/gene="SPO7"
/locus_tag="AWJ20_3991"
/codon_start=1
/product="Nem1-Spo7 phosphatase regulatory subunit SPO7"
/protein_id="XP_018733666.1"
/db_xref="GeneID:30036073"
/translation="MNNIGISDLDESAETRPTQPSGGSEDERVYEEQSSIEIGEITSP
RFTGTEQFGDDVIEGYNNNSSLPALDNQESYLNLNSNSNRDIDTQRASHITSHNQGRN
VASTDSAGIYRSPTNLTSDDTPVDQDISSPIQSSGPVSESTYTTANYIKSAIESNNST
PSSSAPNTALPTPSPTPTPSLTPSPKPIVPAVDNDNPGIDTGAALPTAPVSSGTGASS
TGATETVARRRRKSSTTKSGSKSHSRNSSSISSISAEPIPPNNPTQIFRNLLIMEESF
RDQYIALRRTRRKNVIFFSFLMTAAVYFTYSVFVEPSIYRAVSFFDRLMFLVSIISVG
LFYLTGLYNQAFVLAPKFVHNANKGIRAFNIKLVVVKPTWTESVIRLLWDPVYTAKKG
GLIKLILSSRVFTTDTIETWDLYRERYWLKENERARRRVQNQTLSTSTPAAAASGLGR
KKNTTVSSDGSTALPRRSRLRNTAKTASSHLTVSTGVPSAPSAI"
gene <893079..>896186
/gene="GPI13"
/locus_tag="AWJ20_3992"
/db_xref="GeneID:30036074"
mRNA <893079..>896186
/gene="GPI13"
/locus_tag="AWJ20_3992"
/product="Gpi13p"
/transcript_id="XM_018881036.1"
/db_xref="GeneID:30036074"
CDS 893079..896186
/gene="GPI13"
/locus_tag="AWJ20_3992"
/inference="similar to AA sequence:KEGG_Orthology:K05288"
/note="ER membrane localized phosphoryltransferase; adds
phosphoethanolamine onto the third mannose residue of the
glycosylphosphatidylinositol (GPI) anchor precursor;
similar to human PIG-O protein; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence ISS] [PMID
10793139]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0051377 - mannose-ethanolamine
phosphotransferase activity [Evidence IMP] [PMID
12441642]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_function: GO:0016772 - transferase
activity, transferring phosphorus-containing groups
[Evidence IMP] [PMID 10793139]; GO_process: GO:0006506 -
GPI anchor biosynthetic process [Evidence IEA,IEA];
GO_process: GO:0006506 - GPI anchor biosynthetic process
[Evidence IMP] [PMID 10793139]; GO_process: GO:0006506 -
GPI anchor biosynthetic process [Evidence IMP] [PMID
12441642]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]"
/codon_start=1
/product="Gpi13p"
/protein_id="XP_018733667.1"
/db_xref="GeneID:30036074"
/translation="MSSESVLPNSVPPTSNGNNISPNVLAREAKRKQQAADRRVQKAE
ALRKVNGAWIGAFGITIWVIVLQVAGLLLFKNGFLLSRPVFEDHSECSVLPETQDNIV
SQPSKGCWTEPSFDKAILIVIDALRFDFTIPSDKASPSHFENKLPFLYNEFINNPQNS
LLLKFMADPPTTTLQRLKGLTTGSLPTFIDAGSNFAGTDIAEDNWLAQATNDNKTIAF
VGDDTWTALFPNQFGNISFPYDSLNVWDLDSVDNGVIEHIFPIINGNYGHWDIAVGHL
LGVDHAGHKYGPNHNGMKDKLEQMNQFLENLVASISDDTLLVVMGDHGMDLKGDHGGE
SLLELEAALWMYSKKPFFGRKYGSDIYNTSDSGKNFRSVAQIDFVPTFSLLMGLPIPF
NNLGAPIVEAFIGPDSKNYQALARAEALTAGQIHRYTEKSTSASSAFSESTIQELWHN
VLSADSIGAGLSAAVAYQTAVIDHYREKWIKFHLPDIIRGVLIITASLLSVVIAASNI
NGSNMLQTAKRVTISALSFSAVGFVYRISQDLVYSVLATTSLGIVLGLVWTSRASHAV
SSSSPAPTPTPRSNTQEGQDEQGEEADLGTKKVKETKENSKLSAVPSVWTILAIFLSL
VHAVVFTSNSFTVWESSILNYLLATFGFVAFVYARRLDDVIRRTIATWHCVAFIVLTK
ISSFSVLCREEHGIGCKSTFYQDGSSISSPYALVGLVIVSLALPRIIVSFYKTSESYH
GAAPIWLSIGLQTIMTLSGLFWFLDGAETFNWKVAAAIDHELLHTVKILIARIGLGAS
LVAGNYGWLKGTLCCQLDFEGKRPILKGYYNIYGSFAFLFVLNFFAATLIVNKPMGGI
ILSVLIYHSMTLMEILHLLQIKESIIGPVVFGLLGSAYFFSTGHQATIPSVQWDVGFI
ASKTISFPLTHIAIFLNSLGPFIITALIVPIVPLWKVGPFKSGEAAFTRALKSALAFI
AYHTVVALSSMIWATYFRRHLMVWKIFAPRFMLAAVTLPAVGVSIALALLAVGYTVRQ
VSNIF"
gene complement(<896334..>897368)
/locus_tag="AWJ20_3993"
/db_xref="GeneID:30036075"
mRNA complement(<896334..>897368)
/locus_tag="AWJ20_3993"
/product="hypothetical protein"
/transcript_id="XM_018881037.1"
/db_xref="GeneID:30036075"
CDS complement(896334..897368)
/locus_tag="AWJ20_3993"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733668.1"
/db_xref="GeneID:30036075"
/translation="MDDANGGIGDELDGISLRDISAVRYVRHHEWLELVVGTAIPTHQ
IQIPKIFTPIDADNSSPWSFNDSDSVKAKLESIENEIKQLESGAKSGWVEKPEEAAKS
DFYRAGIAKLREDFGSVSKDADYYGDITLKIETKFGARVIEKKRLRRVGVEVGDEVID
EDEEDKPETKKPALGEDKPQTVEKSDSQQASEISDVSNKSEDFDIKDSGDSNGTGPNI
NKTLQTGTSSTANESENVQTNGDSIGQESTPNSTTEVSDTNDESNFSQSVKTSVPQEY
DQQSEDQPVSEVQSDLLSLDQGEELLLGDDNLVDLDGNNDNVGEDIYMEGTSFEDAEL
GDVLGSLPES"
gene complement(<897589..>900696)
/locus_tag="AWJ20_3994"
/db_xref="GeneID:30036076"
mRNA complement(<897589..>900696)
/locus_tag="AWJ20_3994"
/product="hypothetical protein"
/transcript_id="XM_018881038.1"
/db_xref="GeneID:30036076"
CDS complement(897589..900696)
/locus_tag="AWJ20_3994"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733669.1"
/db_xref="GeneID:30036076"
/translation="MSGNGGANNGIIIETGVIGGDGKVTSTISPPIAVPAGIEASGNS
DNNSVVKGEFSDPNLITLCIPLRYTYLTRPSETTEADYDGRKKIIVYETKSTSLWREA
DEDDKNKQFVKSMSGTQFIDCLPSLRAAVNQINNEKNSLVTARMGEDRLLENIVTVVL
DGPREEVNKQRAKLNRGYFQVERWDIGISKQLVSDSLNEQGDFKSALYLGLEEVAEYC
GVSIFVTSFTDKALFSVNSTEENNYLKHQYDRFDGDDYHILIYGDQDAARFANIRMRV
ILSKLANGSDFIADLVPLELSLQPLVAGPQAVNLHSIQEQASVDIFTMDYLPEVLGSL
NPQLFDGEAIVRDVDQLYIVGPSSANVELAKFLIEQVKTRTQMVVKDCVMSHAKLDLL
ISRYRNKIRDIMNRFATFVQLPYFSSNRSLVRVMGTTASSVEASIQELMRMAADFYEA
NYWIHGGKSDEEGILVRPNGVLNPKSIEALQKIAASNNCIVTTQNNTAFEIIGYGSDT
KRAIAMLKSLSLWDDDISRQNVQISYQLEVSLDLKSFMAGKKYGKMLRIMSNSNVWIK
FIPFNEYNFFVVLTADDYSSSDSGIQLLEDEYPSEISFHIPDTYHKEIIGAGGSGIQA
IMRKHNVFIKFGNSLELYPNAIAQHKLNNVVIRCPSKNAKSMTLARDELLISVRDRVA
EHYNTFVHMPRSHRRIILNENCGIIRQIESKTNSIIQIPDHDNYSDIENDLIEIRGLG
TSSEEASQLLRTALPTDYEFRVAHSSKFTSLVNEKQGDFYTKIVVPFRIAFQFEVMVY
NQVKSKKGDETAPPYHRIVISHSHEHIMGLDEAIQELTTFLRERELDIIDRSRVKDDP
IEYGSAATLGHKRANSALNVNHNRIPQKNLPHSLPTGPAAVSGGHSIPTGPTTPRRLR
YESKQLPPGVSYGDAFTSYDDRSGYYDSRSSAMYETGLGVAPVGSYRDHPLSRQLEAE
TNRQFQSRAYDGVSESAGNFPSGFTDRWQDDEVYEPRRRDRSPPAAPKYNESTIRKRG
YGSYY"
gene complement(<901035..>903077)
/locus_tag="AWJ20_3995"
/db_xref="GeneID:30036077"
mRNA complement(<901035..>903077)
/locus_tag="AWJ20_3995"
/product="hypothetical protein"
/transcript_id="XM_018881039.1"
/db_xref="GeneID:30036077"
CDS complement(901035..903077)
/locus_tag="AWJ20_3995"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733670.1"
/db_xref="GeneID:30036077"
/translation="MPPSTLQLRCYSQWIRRKPAGRSCLFCGYGIMEQRRGIVVVPRH
PKYRAVTPAPTRTGYRDLAPDPDTALPHDTNNINHSRYNNQLRTESESKVRDLTNSER
AWPSIDVETRLDFQEFKMAALSLSFDMSEDLSYRMAPTNEPLESIASNHAISDQEDIT
TLQLSKHSEINLVEQYDNVLNSLINDHLDIFTNYFASNLSSETSISSMAIVQLASQEI
NSDYLRVRAKRSKLKRSPCPASLYNNFLRQLYWNGDGGPPSPRYIFQAYKRLPYPPPR
SINPVHLETLIAFFMGHASRSDPTMYLTILSDIAESGMPISIHEHSAALISLGKQLDK
TDKKHHAQILIEERVADLESQGSIGRNNDSQQELNKGLDTRDPLKFGTNDSNVHALNA
LLSIALKSGSSSSAVEKIFSELSGRADRFTRVLMLSYYGKKRDGRQVKTQYRNIIDSG
DIIDMPVLNLVMKYFLLCGYVEEAESMLHKLVRQKSKAEGSQIMGSHSHRKLESQLRS
RVTIMDYLLKILRDKNIDVGRRYSVPLVPDFYTFGNFFQYYCLGVGNFNKSVEILRLM
TEEAGYLPDKHIFINFYKAFYLHHHNMANEWTLQRLNYITELVCHYELLQAEKHGEHF
CTTNMAVAAFRAYRMLAPTSNTDFDALERQLSKKGSRKRFYNVFTTLKKLSTTRLE"
gene <907621..>908304
/gene="SVP26"
/locus_tag="AWJ20_3996"
/db_xref="GeneID:30036078"
mRNA <907621..>908304
/gene="SVP26"
/locus_tag="AWJ20_3996"
/product="Svp26p"
/transcript_id="XM_018881040.1"
/db_xref="GeneID:30036078"
CDS 907621..908304
/gene="SVP26"
/locus_tag="AWJ20_3996"
/note="Integral membrane protein of the early Golgi
apparatus and ER; involved in COP II vesicle transport;
may also function to promote retention of proteins in the
early Golgi compartment; GO_component: GO:0030134 - ER to
Golgi transport vesicle [Evidence IDA] [PMID 16957051];
GO_component: GO:0005794 - Golgi apparatus [Evidence
IEA,IEA]; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IDA] [PMID 14562095]; GO_component: GO:0030173 -
integral component of Golgi membrane [Evidence IDA] [PMID
16107716]; GO_component: GO:0030176 - integral component
of endoplasmic reticulum membrane [Evidence IDA] [PMID
16957051]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_function: GO:0097020 - COPII adaptor activity
[Evidence IMP,IPI] [PMID 20236934]; GO_process: GO:0006888
- ER to Golgi vesicle-mediated transport [Evidence
IDA,IMP] [PMID 16957051]; GO_process: GO:0031505 -
fungal-type cell wall organization [Evidence IGI,IMP]
[PMID 16107716]; GO_process: GO:0006486 - protein
glycosylation [Evidence IMP] [PMID 16107716]; GO_process:
GO:0045053 - protein retention in Golgi apparatus
[Evidence IMP] [PMID 16107716]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Svp26p"
/protein_id="XP_018733671.1"
/db_xref="GeneID:30036078"
/translation="MVVLTLLSYLGTLSAFVFLTMAVASGLYYLSEQVEEHTVWTKTV
LTRAIYGIIGIHVLLLIFDGFPWLLTVGSIATHILYLQNLKRFPFIQLYSGIFISSCI
AVVANHWLWFRYFSDPSIPPAIILIERPDYAGPTHPPFSQVASFFGICVWLIPFSLFI
SLSVGDNVLPTSYSTNFDNDQDESGAAKAKRRSAGLAKVAVEWFYKVIADIGRKFGYE
IETGKGPLI"
gene <909451..>910419
/gene="PRS2"
/locus_tag="AWJ20_3997"
/db_xref="GeneID:30036079"
mRNA <909451..>910419
/gene="PRS2"
/locus_tag="AWJ20_3997"
/product="ribose phosphate diphosphokinase subunit PRS2"
/transcript_id="XM_018881041.1"
/db_xref="GeneID:30036079"
CDS 909451..910419
/gene="PRS2"
/locus_tag="AWJ20_3997"
/inference="similar to AA sequence:KEGG_Orthology:K00948"
/note="5-phospho-ribosyl-1(alpha)-pyrophosphate
synthetase, synthesizes PRPP; which is required for
nucleotide, histidine, and tryptophan biosynthesis; one of
five related enzymes, which are active as heteromultimeric
complexes; PRS2 has a paralog, PRS4, that arose from the
whole genome duplication; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_function:
GO:0005524 - ATP binding [Evidence IEA]; GO_function:
GO:0016301 - kinase activity [Evidence IEA]; GO_function:
GO:0000287 - magnesium ion binding [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_function: GO:0004749 - ribose phosphate
diphosphokinase activity [Evidence IEA,IEA]; GO_function:
GO:0004749 - ribose phosphate diphosphokinase activity
[Evidence IGI] [PMID 15280369]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0006015 - 5-phosphoribose 1-diphosphate biosynthetic
process [Evidence IEA]; GO_process: GO:0006015 -
5-phosphoribose 1-diphosphate biosynthetic process
[Evidence IMP] [PMID 10212224]; GO_process: GO:0006015 -
5-phosphoribose 1-diphosphate biosynthetic process
[Evidence IGI] [PMID 15280369]; GO_process: GO:0044249 -
cellular biosynthetic process [Evidence IEA]; GO_process:
GO:0031505 - fungal-type cell wall organization [Evidence
IPI] [PMID 15470112]; GO_process: GO:0009165 - nucleotide
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]; GO_process:
GO:0009156 - ribonucleoside monophosphate biosynthetic
process [Evidence IEA]"
/codon_start=1
/product="ribose phosphate diphosphokinase subunit PRS2"
/protein_id="XP_018733672.1"
/db_xref="GeneID:30036079"
/translation="MSVSTNSIKLLAGNSHPELAKLVSQKLGVELSKIQVSQYSNQET
SVTIGESVRDEDVFILQTGCGEVNDYLMELLIIIHACKTASARRITAVIPNFPYARQD
KKDKGRAPITAKLMANMLQTAGCNHVITMDLHASQIQGFFEVPVDNLYAEPTVLRYIR
DQVDYSNSVIVSPDAGGAKRAAALADRLDLNFALIHKERQKANEVSRMVLVGYVQDKV
CILVDDMADTCGTLVKAAETLISHGAKKVIAIVTHGIFSGDAVNRINNSPLDKVVSTN
TVPHDNIQAQCPKLDHIDISPTLAEAIRRLHNGESVSYLFTHAPLG"
gene complement(<910743..>912932)
/gene="SLY41"
/locus_tag="AWJ20_3998"
/db_xref="GeneID:30036080"
mRNA complement(<910743..>912932)
/gene="SLY41"
/locus_tag="AWJ20_3998"
/product="Sly41p"
/transcript_id="XM_018881042.1"
/db_xref="GeneID:30036080"
CDS complement(910743..912932)
/gene="SLY41"
/locus_tag="AWJ20_3998"
/inference="similar to AA sequence:KEGG_Orthology:K15283"
/note="Protein involved in ER-to-Golgi transport;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IDA] [PMID 23613772];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_function: GO:0005215 - transporter activity
[Evidence IEA]; GO_process: GO:0006888 - ER to Golgi
vesicle-mediated transport [Evidence IGI] [PMID 1903839];
GO_process: GO:0006810 - transport [Evidence IEA,IEA]"
/codon_start=1
/product="Sly41p"
/protein_id="XP_018733673.1"
/db_xref="GeneID:30036080"
/translation="MSTLSILHVQDFNKTISVDSSQYGETVSRTQTPLQAKWDIVPIR
DCNSSVLLSPRAENLPSPSRHNYPPRRTRSQTELVPASSGLAKSVSSPGTSISSPSTP
ANIGIPQAGYVAIFNLPDPSLTVMAGGGARSATATTSGTGPISGTASQLSSSATFSLR
GGGMDPGSSGTRARTNSGPPRPAIIKTTFVDQNNSSNGSDDTSSSPLTLNSASSGTSI
GTGFHNVEAVTGSVHGVSGKNPHVRHRDSLSLNGSSAQNFGVMPVTSGTKTATGSAAS
AGGAYEPGSLTGPVNGSVFSSDSSEETLVAALSPVGAPPGGLSSSFIPNHNNQHLHHI
HNQRATSGLLPLAGGNAARRHRSKSFSETVSETSQEAIRQLAPPISLPLVLTCIAWYS
TSAMSNTLNKSILTAFPYPVSLSMVQFTLAASFGISTMKLAQISPDIALALPKGIISP
GRVGFRPPTKAILMATAPMGMFQLSGHILSHMSTSLIPVSLVHTIKALSPLFTVAAYR
LLFGVSYKRSTYLSLIPLTLGVIMTCSVEFSSHFMGLVYALIAALIFVSQNMFSKKLL
TSGNTGGDESTKLDKLNVLCYCSSLAFIFTFPLWFFTEGIGLLGDYSRSSGNFFGASS
NGVDAGSIMSTSGLMLAFLINGFVHFAQNLLAFQVLGMVSPVTYSVASLIKRIVVITV
AIIWFGQRVTGTQGWGILLTFLGLYLYDRCGGDKTKNYEPKSSVLPK"
gene complement(<914141..>916000)
/gene="FRA1"
/locus_tag="AWJ20_3999"
/db_xref="GeneID:30036081"
mRNA complement(<914141..>916000)
/gene="FRA1"
/locus_tag="AWJ20_3999"
/product="Fra1p"
/transcript_id="XM_018881043.1"
/db_xref="GeneID:30036081"
CDS complement(914141..916000)
/gene="FRA1"
/locus_tag="AWJ20_3999"
/inference="similar to AA sequence:KEGG_Orthology:K01262"
/note="Protein involved in negative regulation of iron
regulon transcription; forms an iron independent complex
with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to
repress transcription of iron regulon and is defective in
spore formation; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005829 -
cytosol [Evidence IDA] [PMID 18281282]; GO_function:
GO:0004177 - aminopeptidase activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA,IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0008237 - metallopeptidase
activity [Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0008233
- peptidase activity [Evidence IEA]; GO_process:
GO:0034396 - negative regulation of transcription from RNA
polymerase II promoter in response to iron [Evidence
IGI,IMP] [PMID 18281282]; GO_process: GO:0006508 -
proteolysis [Evidence IEA]"
/codon_start=1
/product="Fra1p"
/protein_id="XP_018733674.1"
/db_xref="GeneID:30036081"
/translation="MVDTSARLSALRSQLSKNNIGYYIVLTQDEHQSEYTSNADDRRE
FISGFTGSAGTAVIGHSKAVLATDGRYFLQAASQLDSNWELLKQGVKGVPTWQEWVAT
EATKTGSNIAVDPKLISYSEVKSLKSLLATKGGKAELISLEDNLIDLVWANDRPSRPL
TPVKILHSEYTGKSFQEKLTQLKDEIIKKNGTAFVLSALDDVAWLFNLRGEDIPYNPV
FFAYAIVTADGKASLYIDSNKVTEKVAEYLKSGNVSIYPYEKAFEDAAELGAALLRIN
KSTSGEKKKLIIPNAASWALVEALGGPDSVQMIESPVTVAKSVKNSTEVEGAKLAHIK
DGVAVIRYLAWLEKALTVDKLVLSDYEAAQKSLEFRSQMENFQGLSFETISSSGPKAA
VIHYSPATDSKEIVKLDQIYLLDSGGQYLEGTTDTTRTVHYGEPSDDEKRSYTLVLKG
HIALARAVFPEGSNGYMLDTLARQHLWKYGLDYRHGTGHGIGAFLNVHEGPMGIGFRP
AYQNAPLQVGNVISNEPGYYEDGKYGIRIESVVVVKEVKTPFNFGDTKFFGFETITQV
PLCQKLLDLSLLDVEEKQWINEYHASVYKTVRPYFSEEDPALEWLRRETLPIF"
gene complement(<916502..>917815)
/gene="ERG11"
/locus_tag="AWJ20_4000"
/db_xref="GeneID:30036085"
mRNA complement(<916502..>917815)
/gene="ERG11"
/locus_tag="AWJ20_4000"
/product="sterol 14-demethylase"
/transcript_id="XM_018881047.1"
/db_xref="GeneID:30036085"
CDS complement(916502..917815)
/gene="ERG11"
/locus_tag="AWJ20_4000"
/inference="similar to AA sequence:KEGG_Orthology:K05917"
/note="Lanosterol 14-alpha-demethylase; catalyzes the C-14
demethylation of lanosterol to form 4,4''-dimethyl
cholesta-8,14,24-triene-3-beta-ol in the ergosterol
biosynthesis pathway; member of the cytochrome P450
family; associated and coordinately regulated with the
P450 reductase Ncp1p; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IDA] [PMID 11914276];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 15951236]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_function:
GO:0020037 - heme binding [Evidence IEA]; GO_function:
GO:0005506 - iron ion binding [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0004497 - monooxygenase activity [Evidence
IEA,IEA]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA]; GO_function: GO:0016705 -
oxidoreductase activity, acting on paired donors, with
incorporation or reduction of molecular oxygen [Evidence
IEA]; GO_function: GO:0008398 - sterol 14-demethylase
activity [Evidence IEA]; GO_function: GO:0008398 - sterol
14-demethylase activity [Evidence IDA] [PMID 9087488];
GO_process: GO:0070988 - demethylation [Evidence IEA];
GO_process: GO:0006696 - ergosterol biosynthetic process
[Evidence IMP] [PMID 200835]; GO_process: GO:0006629 -
lipid metabolic process [Evidence IEA]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]; GO_process: GO:0006694 - steroid biosynthetic
process [Evidence IEA]; GO_process: GO:0008202 - steroid
metabolic process [Evidence IEA]; GO_process: GO:0016126 -
sterol biosynthetic process [Evidence IEA]"
/codon_start=1
/product="sterol 14-demethylase"
/protein_id="XP_018733675.1"
/db_xref="GeneID:30036085"
/translation="MKIHTGRVQTTFDMNIITVHPENIKTILATNFKDYSLGVRYQQL
FPLLGNGIFTLSGEGWKHSRAMLRPQFSREQVSQIDTLRAHVSQLIHNFKTKSKNGTE
FFDAQVQFHNLTIDTATEFLFGESTDSLADSDKKVQGPTRLVSATDFAEHFTAALDVL
ALRTHVGPLYWLVDGWKFRNSIDICHNFVTYFVNKALQNPLEKGTETTGRYVFINELT
KETRDPIVIRDQAFNILLAGRDTTASLLSFIVFYLARDKRVWNTLRQAVLEEFGTEVE
SITFESLKRCTYLNYVINEVLRLHPIVPINFRTAIRDTVLPKGGGPDGNDTLFVPKGT
KIVYTVYTTQRLKEFWGEDSEEFRPERWAEGKSHTWDYLPFNGGPRICIGQQFALTET
GFTLVRIIQTFKDIIYQGSPSYENLSQWTKLTASVADGVWVSFVE"
gene <920607..>923210
/gene="KAP122"
/locus_tag="AWJ20_4001"
/db_xref="GeneID:30036086"
mRNA <920607..>923210
/gene="KAP122"
/locus_tag="AWJ20_4001"
/product="Kap122p"
/transcript_id="XM_018881048.1"
/db_xref="GeneID:30036086"
CDS 920607..923210
/gene="KAP122"
/locus_tag="AWJ20_4001"
/note="Karyopherin beta; responsible for import of the
Toa1p-Toa2p complex into the nucleus; binds to
nucleoporins Nup1p and Nup2p; may play a role in
regulation of pleiotropic drug resistance; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 10684247];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0005635 -
nuclear envelope [Evidence IEA]; GO_component: GO:0005643
- nuclear pore [Evidence IDA] [PMID 10684247];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_function: GO:0008565 - protein transporter activity
[Evidence IMP,IPI] [PMID 10525531]; GO_process: GO:0006606
- protein import into nucleus [Evidence IDA,IMP] [PMID
10525531]; GO_process: GO:0008361 - regulation of cell
size [Evidence IMP] [PMID 12089449]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Kap122p"
/protein_id="XP_018733676.1"
/db_xref="GeneID:30036086"
/translation="MITFDDKDVDQLGSQLLAQLSAEDLNLILRFCKTLLDDYKSNSA
SDLKNAEVQATLISNLPYFLKIAQISFIGVEGAGGVNSSDNKVVELLLQCLETTSTWI
NHFLKYTEAEAQLKHFVPWFLTHLEASFSLQDDSIFKEICDMVVEMFRYNQNVWDRST
RERLYQFTFSVSPTILPPLLTLTEGLDDRNISYCRLVISIAEFLTPKYLQDPDNNGSF
KQLLELLIAFSSIQPTPMVEDTMAMEILEFWNGFVEEIVGGGYLSSEQPQTGYINQII
GIYWNRIMLPPDNITNTWEKDTWQEFLSFRRDFCDFLELAYPIVSSKLFGLLVNSIVE
NIQQSHKTDGTRDPSSMNWRQIECSLYCLNGLSEMIGTSGDEYSTVQLVFNSSLWEDL
SSCNSLRVRQTAVSLIGSFDTFFERPEGQPYLTTTLSYLFKSLSIPDLSLTASRSIQK
LCASSRKHLVSLLPTLMDLYCSRQYYKTTGTVAHERTVNAIACVIQALDNLEEKAQYA
DRLVSILVEQLPVTIENKSQLTDPEVMLLVVSLLKCIVNIGKGLRIPEETASPNREEL
QSLATFWNTYSSSTREKIVGTIQWLTMDAIPFSQDPQICEVCCDIFKSGFSEIVPNPF
VFDPDTILTFISAKHQYGPPTCYTTLIELSSCLVTSSSVGESTLATEYISELLQTFFS
STHENDYEPDIQIGNMQLLTQIYSHYPDSFLSNQSALPFLIGFATEMLASNDRFVIRA
TSKFWTTVITKDNARNETSRTRRSEILHSAGPLIVDKVVGKISGEAIRSDLECYTDVI
KKLVNKYPLESKPWFVNSIINNPRPDLAKQDLSLRTRFLTKIFNLRGSRETNEVVKNF
WSDCKGIANYT"
gene complement(<923609..>924694)
/gene="MAK32"
/locus_tag="AWJ20_4002"
/db_xref="GeneID:30036087"
mRNA complement(<923609..>924694)
/gene="MAK32"
/locus_tag="AWJ20_4002"
/product="Mak32p"
/transcript_id="XM_018881049.1"
/db_xref="GeneID:30036087"
CDS complement(923609..924694)
/gene="MAK32"
/locus_tag="AWJ20_4002"
/note="Protein necessary for stability of L-A
dsRNA-containing particles; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0003674
- molecular_function [Evidence ND]; GO_process: GO:0044419
- interspecies interaction between organisms [Evidence
IMP] [PMID 3551911]"
/codon_start=1
/product="Mak32p"
/protein_id="XP_018733677.1"
/db_xref="GeneID:30036087"
/translation="MGESMRREDVTPVFTSLGMFIIDEIHFTDAPSVYNVIGGAGTFG
AIGARLFLPSPTESQKVGWIVDMGSDFPGSVREEIERWQTGVVLRSDASRLTTRGWNQ
YADNEERLFKYLAPKLRITAADIIANPPLIKSKSFHLICSPNRCEELTRHIVDASNYD
PRAIIIWEPVPDECKPETYGECIKVLRSPGLVSIFSPNAKEAAQFFGLAEPMTKKDIE
DVVDRFVNALNDSRIEPGTNSNMCKLIVLRCGSLGCLVCYTDISNRQPYKWFPAYHNP
ANEDYRVIDPTGGGNSFVGGFATGYVISGGDIVEAAVYGNVAAGLVIEQIGLPILGKD
QDGNEIWNGETISTRIARYRQRSELPS"
gene complement(<925146..>927719)
/gene="NUP116"
/locus_tag="AWJ20_4003"
/db_xref="GeneID:30036088"
mRNA complement(<925146..>927719)
/gene="NUP116"
/locus_tag="AWJ20_4003"
/product="FG-nucleoporin NUP116"
/transcript_id="XM_018881050.1"
/db_xref="GeneID:30036088"
CDS complement(925146..927719)
/gene="NUP116"
/locus_tag="AWJ20_4003"
/inference="similar to AA sequence:KEGG_Orthology:K14297"
/codon_start=1
/product="FG-nucleoporin NUP116"
/protein_id="XP_018733678.1"
/db_xref="GeneID:30036088"
/translation="MFGATNTSPFGATNNATNTGTGLFGSTNNNTATSPFGASTGTQS
SLFGAKPATATGGMFGSNTATTTNNAFGGTSNTGAFGSSGGFGTNTNTNSTSPFGSTF
GANNNSTTNTGGGLFGQQAAKPAFGGFGATNNTATTSPFGTTTNNTSGGLFGQNNNAG
NTGTGLFGANNTNTSSPFGSASSTTNAPAASSGFGGFGGTATQQQNKPAFGFGNSGTT
NTTTTSPFGQSTTNTGTGLFGQTNTANTGGSGLFGNTSSQNTNTGTGLFGQNNTAGGA
TGTSGTGLFGQSNNNATSNTASPFGGFGAANNQQNTGATTGGLFSNAAKPAGSLFGGG
ATTTAPATSGLFGGSTPGATATGSFGVDGGANSAKPSLFGTAPAATAKPTTGLFGGAN
NPLGTRGTFGAPNDTTPRFGLAGNTSAQQQGTSLFGQAAKPSLFSQPSNASTNGGLSL
SGSTGATQLNNSQLLASVDQNPYGNNPLFTSVNGQQAQSQPGTPVATPLSTTPSSNRK
KQGIVSAYKLAPKPLFSPRPRHVGSGSPGNRSSIMGSSATGADDSINGDRTLGKLTRT
GSNSPNAVANSVYSLHADEAILSSNAFSPHGSSVRRLIIDRKRTPNNSLLLTDGRSVA
DESSQLLTPGPSNTSVLPGSIASLDLSETTIPGTPSPNGALRTSVSPEKPFNRGMNNN
GVANDSATTASAPAKPKPVLPDNEIVDEHGYWISPSQEKLDHMSLKELEGVKNFTVGR
KGFGFVRFDVPIDLSQYSSVFKQIPGRIVIFGKKSCVIYPDTSSKPAPGSELNVKATI
SLEGVWIMTKDTREPVKDPNHPLYDKHLQRLKNLKFTTFVSWDKETGTWVFKIDPSAE
S"
gene complement(<927802..>928641)
/gene="NUP116"
/locus_tag="AWJ20_4004"
/db_xref="GeneID:30036089"
mRNA complement(<927802..>928641)
/gene="NUP116"
/locus_tag="AWJ20_4004"
/product="FG-nucleoporin NUP116"
/transcript_id="XM_018881051.1"
/db_xref="GeneID:30036089"
CDS complement(927802..928641)
/gene="NUP116"
/locus_tag="AWJ20_4004"
/inference="similar to AA sequence:KEGG_Orthology:K14297"
/codon_start=1
/product="FG-nucleoporin NUP116"
/protein_id="XP_018733679.1"
/db_xref="GeneID:30036089"
/translation="MFGNTNRVGGFGTSNTTNTTGSGGLFGSSPSTGAFGSSTTGQAT
SPFGQAASSTGGTGLFGQTTNNSGGGLFGSSTSGQTSNVFGSANSANNASSTSPFGQA
NTGASGGLFGATKPTTGFGGFSNTATNSSPFGQSTAATSFGSSANNNQNQGTSVVPFE
PFSEKDTTKANMSNYYQSITSMPQYQNFSFEVSIRYYIYNGPIIELVLRIYSLKLHKF
NHRYGNHKIVQLNCGPTSCFQLTVNGLNGQKISSKFDSLLTHKGIEVSGLSTKSSLWK
RWC"
gene <929516..>930553
/gene="RAD27"
/locus_tag="AWJ20_4005"
/db_xref="GeneID:30036090"
mRNA <929516..>930553
/gene="RAD27"
/locus_tag="AWJ20_4005"
/product="multifunctional nuclease RAD27"
/transcript_id="XM_018881052.1"
/db_xref="GeneID:30036090"
CDS 929516..930553
/gene="RAD27"
/locus_tag="AWJ20_4005"
/inference="similar to AA sequence:KEGG_Orthology:K04799"
/note="5' to 3' exonuclease, 5' flap endonuclease;
required for Okazaki fragment processing and maturation,
for long-patch base-excision repair and large loop repair
(LLR), ribonucleotide excision repair; member of the S.
pombe RAD2/FEN1 family; relocalizes to the cytosol in
response to hypoxia; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 22932476]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA,IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 19699691];
GO_component: GO:0005730 - nucleolus [Evidence IEA];
GO_component: GO:0005654 - nucleoplasm [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 19699691]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22932476]; GO_function: GO:0008409 -
5'-3' exonuclease activity [Evidence IEA]; GO_function:
GO:0008409 - 5'-3' exonuclease activity [Evidence IDA]
[PMID 9166764]; GO_function: GO:0017108 - 5'-flap
endonuclease activity [Evidence IEA]; GO_function:
GO:0017108 - 5'-flap endonuclease activity [Evidence IDA]
[PMID 11825897]; GO_function: GO:0003677 - DNA binding
[Evidence IEA,IEA]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0004519 -
endonuclease activity [Evidence IEA]; GO_function:
GO:0004527 - exonuclease activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016788 - hydrolase activity, acting
on ester bonds [Evidence IEA]; GO_function: GO:0000287 -
magnesium ion binding [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0004518 - nuclease activity [Evidence
IEA,IEA]; GO_process: GO:0000737 - DNA catabolic process,
endonucleolytic [Evidence IEA]; GO_process: GO:0006281 -
DNA repair [Evidence IEA,IEA]; GO_process: GO:0006260 -
DNA replication [Evidence IEA]; GO_process: GO:0043137 -
DNA replication, removal of RNA primer [Evidence IEA];
GO_process: GO:0043137 - DNA replication, removal of RNA
primer [Evidence IDA] [PMID 16837458]; GO_process:
GO:0006284 - base-excision repair [Evidence IEA];
GO_process: GO:0006286 - base-excision repair, base-free
sugar-phosphate removal [Evidence IGI,IMP] [PMID 9927726];
GO_process: GO:0006974 - cellular response to DNA damage
stimulus [Evidence IEA]; GO_process: GO:0006303 -
double-strand break repair via nonhomologous end joining
[Evidence IDA] [PMID 15342630]; GO_process: GO:0000734 -
gene conversion at mating-type locus, DNA repair synthesis
[Evidence IMP] [PMID 10025407]; GO_process: GO:0035753 -
maintenance of DNA trinucleotide repeats [Evidence
IGI,IMP] [PMID 16079237]; GO_process: GO:0090305 - nucleic
acid phosphodiester bond hydrolysis [Evidence
IEA,IEA,IEA]; GO_process: GO:0001302 - replicative cell
aging [Evidence IMP] [PMID 12024027]"
/codon_start=1
/product="multifunctional nuclease RAD27"
/protein_id="XP_018733680.1"
/db_xref="GeneID:30036090"
/translation="MSLYQFLIAVRQQDGQQLTNEDGETTSHLIGIFYRTLKIVENGI
KPVYVFDGKPPEMKGSELAKRLVRREEAEKQAAELKETGTAEDVSRFERRTVRVSKEQ
NEEAQKLLKLMGIPFVLAPGEAEAQCAALAAADKVFASASEDMDTLTFGTPFLLRHLT
ASDQKKMPIAEVNLKKAMEGLEMSKDEFIDLCILLGCDYCDPIRGVGPTTALKLIQEH
RSIEKIKAALASKESSKVQIPDDWPYEEVRKLFHSPDVMPPSEIDLKWGVPDVEGLVD
FLVKEKGFSEDRVRNAAAKLTKGAKSTPQGRLTDFFKPKPVDPAKAAEMAKRKKAAAA
ADNKVKKKARK"
gene <931642..>932619
/gene="APN1"
/locus_tag="AWJ20_4006"
/db_xref="GeneID:30036091"
mRNA <931642..>932619
/gene="APN1"
/locus_tag="AWJ20_4006"
/product="DNA-(apurinic or apyrimidinic site) lyase APN1"
/transcript_id="XM_018881053.1"
/db_xref="GeneID:30036091"
CDS 931642..932619
/gene="APN1"
/locus_tag="AWJ20_4006"
/inference="similar to AA sequence:KEGG_Orthology:K10771"
/note="Major apurinic/apyrimidinic endonuclease; 3'-repair
diesterase; involved in repair of DNA damage by oxidation
and alkylating agents; also functions as a 3'-5'
exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine;
genetically interacts with NTG1 to maintain mitochondrial
genome integrity; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 11238901]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 7690756]; GO_function:
GO:0017005 - 3'-tyrosyl-DNA phosphodiesterase activity
[Evidence IDA] [PMID 12397185]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0003906 -
DNA-(apurinic or apyrimidinic site) lyase activity
[Evidence IEA]; GO_function: GO:0003906 - DNA-(apurinic or
apyrimidinic site) lyase activity [Evidence IDA] [PMID
12767225]; GO_function: GO:0003906 - DNA-(apurinic or
apyrimidinic site) lyase activity [Evidence IMP,ISS] [PMID
1693433]; GO_function: GO:0008311 - double-stranded DNA
3'-5' exodeoxyribonuclease activity [Evidence IDA] [PMID
16024777]; GO_function: GO:0004519 - endonuclease activity
[Evidence IEA]; GO_function: GO:0016829 - lyase activity
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0008081 -
phosphoric diester hydrolase activity [Evidence IDA] [PMID
3056935]; GO_function: GO:0008270 - zinc ion binding
[Evidence IEA]; GO_process: GO:0000737 - DNA catabolic
process, endonucleolytic [Evidence IEA]; GO_process:
GO:0006281 - DNA repair [Evidence IEA,IEA]; GO_process:
GO:0006284 - base-excision repair [Evidence IDA] [PMID
9295360]; GO_process: GO:0006974 - cellular response to
DNA damage stimulus [Evidence IEA]; GO_process: GO:0090305
- nucleic acid phosphodiester bond hydrolysis [Evidence
IEA]"
/codon_start=1
/product="DNA-(apurinic or apyrimidinic site) lyase APN1"
/protein_id="XP_018733681.1"
/db_xref="GeneID:30036091"
/translation="MHPGSTLGSDKPSAIKQIGDSINEMHGKTEFVKIVLENMTGQDR
IVGSNIADLRDIITYVKNKERVGVCIDTCHSFCAGYDLRTENTFNKFWDLFDKTVGYK
YLSGIHLNDSKFPFDSKRDVHYNIGLGFLGLEAFRLIMNKEELQNIPLILETPIEHED
GKTPDDDPRADEIALLRWLVGKEKDDKEVLDKSIQLQKLGAKVREKNLLVVDKKKPIS
SKKPVKNDGTITFAKAKKVKTEVQERNLENQETALESELELKDEQATIVKAEAKDGPD
PEVDQTDVAAIGAASVPELSVDDNKLTSRKRATRVSTSTRKTKVRKPSA"
gene <933560..>935002
/locus_tag="AWJ20_4007"
/db_xref="GeneID:30036092"
mRNA <933560..>935002
/locus_tag="AWJ20_4007"
/product="arrestin (or S-antigen), N-terminal domain
protein"
/transcript_id="XM_018881054.1"
/db_xref="GeneID:30036092"
CDS 933560..935002
/locus_tag="AWJ20_4007"
/codon_start=1
/product="arrestin (or S-antigen), N-terminal domain
protein"
/protein_id="XP_018733682.1"
/db_xref="GeneID:30036092"
/translation="MTYLHRPQAPPYIETDQTVLADDDNSFDASHNLPEYTSTTTCEI
TTNPQRSIYDEVLRVSIDTEVVASRRNIFDVGDVLTGSVTVAPIKNMEFYLLLVDLVG
TEMSIRGKGYAETRYTRVFSLARYTLSQRTFSGVMEQGSKYKFTYSMIIPDKIPHERC
VCGNNQHSQVPPSIGRPFELTTPCGGEHLVSEQFGRILYQVRAKLVKPETLWKSSAPE
FWSEGRKYLVIRPSYPPPMLEQLCDVNGKPLRLPVYMSSKSLKKGLVRKTDIGKIELR
TSEALDVYLGVPHTNVLNLEVLYTPSPNLSGVFPPPVEIENVSYQVDSLAFSSTQSMV
TYPSHESVIMKTDPCTILSKHTLLRQKLTISKPAWTVSTPKDPSEPRSYLTKLTLPFT
FPVYHSRVVDTYFSCLTSRQYELIVSVGFGHSIGSHRLTVPVLVSSAKDVTLSVAPSF
SSIDTTSNLSTYTDLSIPSYQETFDTVDSS"
gene <937968..>939551
/locus_tag="AWJ20_4008"
/db_xref="GeneID:30036093"
mRNA <937968..>939551
/locus_tag="AWJ20_4008"
/product="hypothetical protein"
/transcript_id="XM_018881055.1"
/db_xref="GeneID:30036093"
CDS 937968..939551
/locus_tag="AWJ20_4008"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733683.1"
/db_xref="GeneID:30036093"
/translation="MTIRPLDQLTEDPPSYIEPTQPLLEITDQDDPISSATTDLPRYI
SGTTCEIAPCNTTSIYEPVVGVGIDPSPTRRDPLSLDLGDVITGKVVITPRKSMDFSI
LLVDLVGEEISLRDRGYSESHHKRVFTVARYEIPHQHISGPLEEGFVYTFTYSLVVPE
RLPREFCPCGTGNPIHSQLPPSYGLPLENYTPRNHEENEECARVLYRVRARLYKNLRN
SREETLKIWAQGHKTVSIRSSLYPPLVLEELKDSNGNILRLPVYSTSKQLSHGLIRKV
AIGTVELKTSEPLNIYLNNPSTKLVSLDVIYTPPSDQKSTSPLEIHHVSYKVNALTFA
SIEAMAEIPSRQSVMSNKKSPVYMSKDNILRQNLTICKPLWRLNENNDSKTKGNSYVT
TLKLPFSFPVTNKKVVSTYFSCLTSRQYELTVTVNFGSSIGSHSITIPVLVTNYKSRE
SSVASVYSVEDISPLRSDASSYFSYSIPTYQESVGSSTASSTGAITPPTPGHNFTWGG
GARRIARTCEQAAMIIASI"
gene <940062..>940955
/gene="YPR1"
/locus_tag="AWJ20_4009"
/db_xref="GeneID:30036094"
mRNA <940062..>940955
/gene="YPR1"
/locus_tag="AWJ20_4009"
/product="trifunctional aldehyde reductase/carbonyl
reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1"
/transcript_id="XM_018881056.1"
/db_xref="GeneID:30036094"
CDS 940062..940955
/gene="YPR1"
/locus_tag="AWJ20_4009"
/note="NADPH-dependent aldo-keto reductase; reduces
multiple substrates including 2-methylbutyraldehyde and
D,L-glyceraldehyde, expression is induced by osmotic and
oxidative stress; functionally redundant with other
aldo-keto reductases; protein abundance increases in
response to DNA replication stress; YPR1 has a paralog,
GCY1, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0004032 -
alditol:NADP+ 1-oxidoreductase activity [Evidence IDA,ISS]
[PMID 11306085]; GO_function: GO:0004090 - carbonyl
reductase (NADPH) activity [Evidence IDA] [PMID 19016485];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_process: GO:0042843 - D-xylose
catabolic process [Evidence IDA] [PMID 12271459];
GO_process: GO:0019568 - arabinose catabolic process
[Evidence IDA,IMP] [PMID 12271459]; GO_process: GO:0034599
- cellular response to oxidative stress [Evidence IGI]
[PMID 17919749]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="trifunctional aldehyde reductase/carbonyl
reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1"
/protein_id="XP_018733684.1"
/db_xref="GeneID:30036094"
/translation="MAPTLNKQTVTLNDGSKLPVVGYGTYAVTDETNGYDALLEALKK
GYRHIDTAFIYNNEEVVGKAVKDSGIPRDEIFIVTKIWNNDHQDPAHALERSLRLMEL
DYVDLLLIHWPVPQKAEGGETVVDKEWSHVKTWQLLQELPREKVRSIGVSNYDTVLLE
ELLNAPSTKVLPSVNQVELHPYLPQDKLRDYCQKKKIVLEAYCPLGSGAKLLENPLLK
ELAEKYEATPAQIALSWGISRGYVVLTKSSTPSRIESNIQTVELSPEDITKITNLSKT
HPHRIVVPTWAKGVTFHDDDF"
gene <941544..>943850
/locus_tag="AWJ20_4010"
/db_xref="GeneID:30036096"
mRNA <941544..>943850
/locus_tag="AWJ20_4010"
/product="hypothetical protein"
/transcript_id="XM_018881058.1"
/db_xref="GeneID:30036096"
CDS 941544..943850
/locus_tag="AWJ20_4010"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733685.1"
/db_xref="GeneID:30036096"
/translation="MSTDEGLPPYQVVDERQAWSTNNDNVPASTNAVDGGVTEDKPVS
LNLTENTSGGLPTPDQCIAHLALLEAFYMLRFDIANRDGLFGISNSMAEKFTGDEKTR
AMASLCEKRWATYLVRAEERFSKWWATLPRESPMPTQSEIIANPTVFSYIPITGTKIP
FTRDNLPPADVLMVWHSYMLNPRNYLQDCIRQGMRDVWRTGMPWKALYSCISGDSVYK
SSDGAKNNFKNLTGCEWDNLDDPPVKTLQCPNCKVDIQVPWTSGSLGDDLSKPFENSR
GYGDKNFAVQCYSCSLYINHPFLRLQKFRTDFLLLATKDVPMPGTLLTRDGYPVSSRP
PSESHQSYFFINRLLMFQMNSFDQICDPAITLKEQRQGDLQFEMSSVRKLLEMCLVRR
DIVRKVNSGVVNTQKGLINGKIAVRRMMACYWENSSPFSIDLVGAMTRQGTFIEKMHN
IDWLHSPTVSSTMKYALIKYSRFMDIIATNPSRVAVPTLDVDLAWHTHQLSPSDYYHY
SDKTTHKFIDHDDKIEESKLSDAYEWTCKTYQRLYKEPYSSCMCWYCEAIRETNRSIL
HSRSDAKVDEKLFNHSDISADPNKNPHISAHNAVQTMVVDTVKRAHLRRKEQKMKQLE
KAYMLAQKRAKKKGRNLPDRNYDPYYSSYWGWGYPIIFPLGYAPFFGYPLGGEYLYPS
NPACMSADPGAVGNCCNGTCGGNVAQGGCAGMTGACAGGTVGACAGGGGVGGACGGGL
GGCGGGGGGGGGCGGGGGGGCGGGGGSC"
gene complement(<946717..>948507)
/gene="RFA1"
/locus_tag="AWJ20_4011"
/db_xref="GeneID:30036097"
mRNA complement(<946717..>948507)
/gene="RFA1"
/locus_tag="AWJ20_4011"
/product="replication factor A subunit protein RFA1"
/transcript_id="XM_018881059.1"
/db_xref="GeneID:30036097"
CDS complement(946717..948507)
/gene="RFA1"
/locus_tag="AWJ20_4011"
/inference="similar to AA sequence:KEGG_Orthology:K07466"
/codon_start=1
/product="replication factor A subunit protein RFA1"
/protein_id="XP_018733686.1"
/db_xref="GeneID:30036097"
/translation="MTELTVCFAEFGRTSNPSEVVPKFPQLKLQVLKVKLIQGAEPSQ
NRMRLTVSDGVHYSQAIVNLNEEKGGAQSDISVGSVIEVKSYDSHRLQTGKIVFVLYE
WDVVGVESPIGHPTSLEANTEAKANDSMSPAPAPVAPVVAPPAPAPVTKPKAKPAAAP
GNFTAIIPIDILSPYHNKWTIRARVSNKSDIRTFTNARGEGKLFNVTLVDESGEIRAT
GFSQQVDAFYDLLQEGKVYYISKCRVQVAKKQFSNVQNDFELVFDRDSQIELCTDEVD
VPKIKFDFTSIKDLGTADINGTVDFIGVLKSINPAEQITSKNTGRPYDKRDVVLVDEG
DYSIRATFWGKSAVDFSTPLESVIAIKGAKISDFNGRNISILNSSTVSVDPDVPEAHA
LKGWYSTNGRDKTFEQLPGPSGATSTGNSDDKNRTTIEKVISDQLGMTDQTDYFTIKG
TISYIRNENMWYPSCTNADCNKKVIQDTTDEWRCEKCNITIAEPNYRYIISMIVSDST
GQIWVSSFDDIGNIVIGMSAKELYQLQEQDLNGFTEVITKALYKEYVLRCRARKDNYN
DQVSVRYSAVRCTPVDYAHETQLLLQQLGI"
gene <952156..>954954
/locus_tag="AWJ20_4012"
/db_xref="GeneID:30036098"
mRNA <952156..>954954
/locus_tag="AWJ20_4012"
/product="hypothetical protein"
/transcript_id="XM_018881060.1"
/db_xref="GeneID:30036098"
CDS 952156..954954
/locus_tag="AWJ20_4012"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733687.1"
/db_xref="GeneID:30036098"
/translation="MDYNSQPAGGYPQPQTGVAGAGSNRSTAISHNGMPRSRHQQQLE
DMLLSPGGGVDSHMDDMIQRPGSRANVNVNGPSNTGPGYLPRSASAMSFGRRISTSSV
TSGSGRNSTANNAGFFGWGKKKFSNQDFDDDDDEGGTGAIGKAEDVPDISMNELTGLR
DRDRYPTFGGAPGTPGGPTSPGTGVPSNGRTMSISSTMSGGGFDTTPVIPTLRMGSGT
AGPQGTGQYRKNLTNSLKQAIRSSDGRLVGLSQSSPGQGPVPGPGYGLSGPNGMSGGN
GYPMQQQRPYTQHQRPPSLQNSRPNSDPRLRSLTGPPNHDFIGPPHNGGSARTMSMGG
GIGYFPPNANGIVGPSGPGAQGVIGGSPTMSGSQGFGPGPGPNVGLGQGAGRPTQRLS
RPPSRLSQQHAPMVTASTMTDPIDLGDLPSLPREPGTNANKSSNSSTNSTDSTVDGTV
TTATTMSSVTGSPIKAIPLSAGNNSADLNELRALNKALLDEVRLVTSELADSIRRELG
LSTHEDDSSIDNVSISSSNGDNEIIEARQRAQKLVILERELDLERRKRMVAQQKHEVD
EQHQLLTSNQYKIIELEKELAEKNRLYANERRESEMLRDNVSALTNEYEELQLETNQL
KNGILPELKSHVADLEVLTAAGNPIELLKQIEELKVENKKLQGLVEENSMRGPLGEKI
KAVESQRDALREALRSLKERNDHEIRQSTERVRQLEGKLEKEKVITNQMQRKLVQTRS
PSAYVALNSPYEVPSSANNHHTLSTSSSSGSLNSGLNGSNNVDVGLASSTGLGQNTGG
SGSTLSIPKRRGIGNLPGITPPAMSFSEMRPASPSPVNFEISQDPAWIDYVNPSKLPV
SQAHARQFSSTSYSSQASNSPTSETASLFKTPTYRSNASNSPTTGLSASSLSISNGRT
EHLPLSLAPAAATATTSS"
gene <970817..>972613
/gene="SAS10"
/locus_tag="AWJ20_4013"
/db_xref="GeneID:30036099"
mRNA <970817..>972613
/gene="SAS10"
/locus_tag="AWJ20_4013"
/product="Sas10p"
/transcript_id="XM_018881061.1"
/db_xref="GeneID:30036099"
CDS 970817..972613
/gene="SAS10"
/locus_tag="AWJ20_4013"
/inference="similar to AA sequence:KEGG_Orthology:K14767"
/note="Subunit of U3-containing Small Subunit (SSU)
processome complex; involved in production of 18S rRNA and
assembly of small ribosomal subunit; disrupts silencing
when overproduced; mutant has increased aneuploidy
tolerance; essential gene; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 12068309]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9611201];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0032040 - small-subunit
processome [Evidence IDA] [PMID 12068309]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0000480 - endonucleolytic cleavage in 5'-ETS of
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 15489292]; GO_process:
GO:0000447 - endonucleolytic cleavage in ITS1 to separate
SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic
rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 15489292]; GO_process: GO:0000472 -
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 15489292]; GO_process: GO:0000462 - maturation
of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID 12068309];
GO_process: GO:0000462 - maturation of SSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 12837249]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA]; GO_process:
GO:0042254 - ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Sas10p"
/protein_id="XP_018733688.1"
/db_xref="GeneID:30036099"
/translation="MARKSRGINTSSRRDERDEEELDLDEIDQFHKNQEEILLDQAGL
ARKGRSGLDEEMEMSDEEVLGLDNDEEEDEEDMEMDDDALYGGNNEEDEEEEGWGSSK
NNYYGADEPQDEESAKQEEQEALRLQKAHLAELAEDDYYEDEDLDEWKESAMATESDL
LDSTGGVHHEALPEQDPTLLSETERLNLLQTWYPEVLPFIGELEKYSGLLESLKKTVK
ESENKIASIKFAALSSYLGTLSAYFALFVGTIENGKVSLKDHDVVKGILKARELWRLA
SNLDDEQDESVKNKSKSNSGSSVAVTNASSSDTADDTIWQSASEGYSSEDETATINSK
SSATKRKRAALDRNESGSDSDSEFSVGAPVVKHVKRTRGIKSDYREEDIDEVSIADKL
ARKKSLRFYTSKIDQQTNKANNGREKFSGDADLPYKERHFERQQRLMEEARKRGLKTS
GGAYDPADDFGSDDDNDRSTAKLVNDGFSEDYYNTIKAASEKRKEEKRNNHLLAKQAA
KEGRLEELASELATDGDKRAINYQILKNKGLTPKRNKDNRNSRVKKRKRYEAAKKKLP
SVRQVYKAPTSSYQGEQTGIKKNLIRSVRFAN"
gene <973080..>973805
/locus_tag="AWJ20_4014"
/db_xref="GeneID:30036100"
mRNA <973080..>973805
/locus_tag="AWJ20_4014"
/product="hypothetical protein"
/transcript_id="XM_018881062.1"
/db_xref="GeneID:30036100"
CDS 973080..973805
/locus_tag="AWJ20_4014"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733689.1"
/db_xref="GeneID:30036100"
/translation="MSSSKAFKSHYEQDERWTAVDEYTNGHLHNPDTHGNSKVLSEAL
SNSIDKGLPDISTFPSYGKLLALQAKLAGADYALEVGTLGGYTSIWLATENPNIKIVT
LEVNQHHADVALENLRRAGVADRVEIIVGPALETLPKLAEEIAAGKRQKFGFTFIDAD
KTNNWNYFDWAVKISRSRAVIVVDNVVRNGTIVAKEKLNDPNVSGARNVIERVGKDNR
VDAVVQQTVGEKNYDGFLLAIVK"
gene <976058..>976879
/gene="AIM2"
/locus_tag="AWJ20_4015"
/db_xref="GeneID:30036101"
mRNA join(<976058..976133,976176..>976879)
/gene="AIM2"
/locus_tag="AWJ20_4015"
/product="protein AIM2"
/transcript_id="XM_018881063.1"
/db_xref="GeneID:30036101"
CDS join(976058..976133,976176..976879)
/gene="AIM2"
/locus_tag="AWJ20_4015"
/codon_start=1
/product="protein AIM2"
/protein_id="XP_018733690.1"
/db_xref="GeneID:30036101"
/translation="MRKPAITPRPAGALPQTPLLFWLGTTGESVGRLIDLNGVETYIS
GEKNASSKKAVLFLTDVFGHKYINNQLLADEYAKAGYLVVVPDLFKGDPRSPDSVPGA
DPNELRTKWLPNHTPEITRPIVEKAVEGLKKEYSPEFVGTVGYCFGARYVLQQLATDN
VNAGAIAHPSGVTIEDVRAVRKPLLIIGAETDGAYTPELQKQTEDVLREIKATYFTTL
ASGVSHGFAVRGDITNPIVKFAKEKALRDTVDWFNAFNSRK"
gene <977848..>979965
/gene="mei2"
/locus_tag="AWJ20_4016"
/db_xref="GeneID:30036102"
mRNA <977848..>979965
/gene="mei2"
/locus_tag="AWJ20_4016"
/product="RNA-binding protein involved in meiosis Mei2"
/transcript_id="XM_018881064.1"
/db_xref="GeneID:30036102"
CDS 977848..979965
/gene="mei2"
/locus_tag="AWJ20_4016"
/codon_start=1
/product="RNA-binding protein involved in meiosis Mei2"
/protein_id="XP_018733691.1"
/db_xref="GeneID:30036102"
/translation="MNSLELTRYSLNASVSGPSLDTSSGRIVSTCLRHSSSGNDANNS
PVRFAVGHEVHMATSEGKFGSIQTGVQAVDSSQPGINSRADSLRQIPISDYISCGMHE
KTREPSPTSTSLRSLWFSGPQPVSSSESSRPFNPIGFGSNIYPRDESLSPCQNNHTLF
SLGSVIDSLGLVSGPLVRLEHRIEPIDTSSICADSERQTRLVKVSNLPINPTDQNTVS
FSLSPQSVLESCIAAGGTVPGSTFIEGSTVVALYYDLRDSVRLVDKLRERLAESNRSS
HRVTGNWANIQVAFIPVHEFDIVYRASTFLEHLALHKKVAVHLVIKENMRYCGYTTES
IGQILTKSIGDIRYIRKLPTTYAVVFVCEFFDIRARLNCINATREESSTLPFYCLSSY
SSENNMVKSTEAVSGVSQNQEQQLQPNENICYPHHQLNKSGTIASKPPISIYSTNSPT
SASKRSFASVASSGLNRNNEFVLRGSSDHSGIRPVPDLSGKTLKGKGNENPFGISQKH
FGFQIVDQNSRNGDSTSWEDSQEQIISTTATDTTLPAPIASSSPVLAPKSVAPLLTTI
LSPLSSSQMNRMNADTTSGISGSIVTRAAVPSKSIIDLDRIEQGLDTRKTIMIRNIPN
KIDQQMLKEYIDVTNRNTYDFLYLRIDFNNKCNVGYAFISFIDPRHIITFARARAGTR
WNKFNSAKICDMSYAAIYVESPL"
gene <981659..>983995
/gene="HSL7"
/locus_tag="AWJ20_4017"
/db_xref="GeneID:30036103"
mRNA <981659..>983995
/gene="HSL7"
/locus_tag="AWJ20_4017"
/product="Hsl7p"
/transcript_id="XM_018881065.1"
/db_xref="GeneID:30036103"
CDS 981659..983995
/gene="HSL7"
/locus_tag="AWJ20_4017"
/inference="similar to AA sequence:KEGG_Orthology:K02516"
/note="Protein arginine N-methyltransferase; exhibits
septin and Hsl1p-dependent bud neck localization and
periodic Hsl1p-dependent phosphorylation; required along
with Hsl1p for bud neck recruitment, phosphorylation, and
degradation of Swe1p; relocalizes away from bud neck upon
DNA replication stress; GO_component: GO:0005935 -
cellular bud neck [Evidence IEA]; GO_component: GO:0005935
- cellular bud neck [Evidence IDA] [PMID 10490648];
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 10805747]; GO_component: GO:0005935 - cellular
bud neck [Evidence IDA] [PMID 22842922]; GO_component:
GO:0032174 - cellular bud neck septin collar [Evidence
IDA] [PMID 23389636]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 22842922]; GO_function: GO:0008469 -
histone-arginine N-methyltransferase activity [Evidence
IEA]; GO_function: GO:0008168 - methyltransferase activity
[Evidence IEA,IEA]; GO_function: GO:0035241 -
protein-arginine omega-N monomethyltransferase activity
[Evidence IDA] [PMID 16426232]; GO_function: GO:0035241 -
protein-arginine omega-N monomethyltransferase activity
[Evidence IDA] [PMID 18515076]; GO_function: GO:0035243 -
protein-arginine omega-N symmetric methyltransferase
activity [Evidence IDA] [PMID 18515076]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0000086 - G2/M transition of mitotic cell
cycle [Evidence IGI,IMP] [PMID 10490630]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0051301 - cell division [Evidence IEA]; GO_process:
GO:0044257 - cellular protein catabolic process [Evidence
IMP] [PMID 23042131]; GO_process: GO:0034969 - histone
arginine methylation [Evidence IEA]; GO_process:
GO:0032259 - methylation [Evidence IEA]; GO_process:
GO:0007067 - mitotic nuclear division [Evidence IEA];
GO_process: GO:0006479 - protein methylation [Evidence
IEA]; GO_process: GO:0051726 - regulation of cell cycle
[Evidence IMP] [PMID 8647431]"
/codon_start=1
/product="Hsl7p"
/protein_id="XP_018733692.1"
/db_xref="GeneID:30036103"
/translation="MSDGTSTTSTVSMKPPLFVGVNLPAKHGVSDHLVVEAALDQGYD
MVTSRLTSRSYRKRIQALFHELEMVTPSPDEHLGNQGSNNNHGAFSFSQHQASHSHSH
SYPHRIFSDGADLSPYNSPSMSHSNGVNGARDTAIVSRQSSEPPIQQLPTGTLVEAEI
PAPELEDVVVHPGDHVSNTIALAAPWVEIDSKNPRVANLSLQVLSHELKYATFCGLPY
VIISGPKRRTNVIQYAQALNTLLMANPTLQVSIHLPLTEEFTKSATLVPPSDYLSIWE
VWNTIRNTCNYPHNISVALQLPRHTLPDYVVNRWFAEPVSMIIVSSRSFVRNAKGYPV
LPKSSQTVLNKFFKKNPFLIIEDPQDSTSFVGGDTAFLLYLRHLYQTSPPPSIIEGFA
AGYTDYLQAPLQPLIDNLDSAMYEVFERDPIKYNQYEKAIFHALLDRPGKEVVIGVVG
AGRGPLIDRVLKAASAANRTVKVLALEKNDSAYIYLLKRQAQDWGSAVQIIRGDMRTW
VPPPNTPAVNILVSELLGSFGDNELSPECLDGAQRLLDPVNGITIPSSYSSHITPIFS
PKLFASAWSLSAANSSGSQSGNGPATNSHSIASIRASRPGMCSADALHSPYVVMMEQV
DFVSEKIIQAWTFTHPIQNMLPNNQHNVRKCKATFPVPHKTVVHGIAGYFEAVLYKDI
HLSIRPDTIEAKSKDMVSWFPIWFPLSQPLVLGDDSEVDVSIWRLSDGRSVWYEWAAE
GYATVTMPDTGRGTSVRRIRSGSTAFHNSGGKYSSMTL"
gene complement(<990502..>993891)
/gene="RRP5"
/locus_tag="AWJ20_4018"
/db_xref="GeneID:30036104"
mRNA complement(<990502..>993891)
/gene="RRP5"
/locus_tag="AWJ20_4018"
/product="Rrp5p"
/transcript_id="XM_018881066.1"
/db_xref="GeneID:30036104"
CDS complement(990502..993891)
/gene="RRP5"
/locus_tag="AWJ20_4018"
/inference="similar to AA sequence:KEGG_Orthology:K14792"
/note="RNA binding protein involved in synthesis of both
18S and 5.8S rRNAs; component of both the ribosomal small
subunit (SSU) processosome and the 90S preribosome; acts
as part of a Mak21p-Noc2p-Rrp5p module that associates
with nascent pre-rRNA during transcription and has a role
in bigenesis of the large ribosomal subunit; has binding
preference for single stranded tracts of U's; relocalizes
from nucleolus to nucleus upon DNA replication stress;
GO_component: GO:0030686 - 90S preribosome [Evidence IDA]
[PMID 12150911]; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 22842922]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 8896463]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0032040 - small-subunit processome
[Evidence IDA] [PMID 12068309]; GO_function: GO:0003723 -
RNA binding [Evidence IEA]; GO_function: GO:0003729 - mRNA
binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0008266 - poly(U) RNA binding [Evidence IDA] [PMID
16428605]; GO_process: GO:0006396 - RNA processing
[Evidence IEA]; GO_process: GO:0000480 - endonucleolytic
cleavage in 5'-ETS of tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
8896463]; GO_process: GO:0000447 - endonucleolytic
cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and
LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 8896463]; GO_process:
GO:0000464 - endonucleolytic cleavage in ITS1 upstream of
5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID 8896463];
GO_process: GO:0000472 - endonucleolytic cleavage to
generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 8896463]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA]; GO_process:
GO:0042254 - ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Rrp5p"
/protein_id="XP_018733693.1"
/db_xref="GeneID:30036104"
/translation="MKTPGSIVVLQPKGAVVEFFGGVRGYLPVAEISEAFIKDPKDHF
RLGQSVAVRVLSVDPEAERLLVSCRTDSSNSQAQLESLGPGTVVSASIVEKTKDGVVV
NVEPQNVRGVIPLGHLTDGNDTRAQMKSLKVGENLESLVILERNFSKRFVMLSAKKSL
VEDARAGKFPSDISQISVGDKLHGYVKNATIKGLFVGFAGSLTGLALKQDLSTEFVSD
PAEKFSPMQSVECYVVNIDDAIGRFQLSLLAPKPKTLKKETSSGGFTPVDEAVTSIDE
FVPGKITKAQIVSVKETQLNVKLAENVQGRIDVSQIYDDYKSIPNSKDPLGEFKKGEI
VDVKIIGYHDARNHTFLAISHRSASHLVYELSAKKSDLNSSSYKPLTLEDVKVGSSWL
AFINNLTVDFAWVNLSPTVRGRIPLFDLTNNADELEDVEAHYPIGSAIECSVIEVDIK
TSNLKLSVRAHNGTAVNDYSDVKVGQTLPTLVTKVTETLVFVKLGANISGAAYISDAL
DDYSADLKLTYLPHQLVKAKIIDVDTSNKRISVTLRPSQTEKDAEKPVDQHIQSIEDV
QKGSIVRAFVKNVADKGLFVSLGRNITARIQIKNLSDSYLKDWKKYYTVGQVVTGKVL
SVDEHNSRVELSLKESAVTGKDPREVSGLGSINENDIFDGVITKVEDFGVFVKLDGTS
ITGLCHKSEVADVPLQDMSKVFSEGDKVKAKILKIDTEKRRVSLGLKASYFTELESDV
EMEEFDVNNDEDDDDDDELDNSVAFEDDSEEEEEGDESDDESDEDNGIAVTASKKSSN
GLSVGFDWTGTNVLDEMNTKEDGDDNEDDDSSNEEEGRSRKRRKRSKFVEDKTGTLNT
KLPQSIQDFERLLVGSPNSSIMWMNYMAFQLQLSEIEKAREIAQRALKTINFREEQER
LNVWIALLNLENSFGTNQTAEDTFKEAIQFMDSKTIHMKYAGILSQSEGKAKKAEELY
DVAMKKFGGEDMSIWITYANFLFDHDNAAKARSLLDRSLRMLPARNHKEIITKFAQLE
YTKGEAERGRTLFEGLISSYPRRIDLWIVYLDYEIKNGDKETVEKLFERVIGVKLTMK
QAKFFFKKWLGFEDKLGDSKKVDYVKAKASEYVSSRTEQEE"
gene complement(<994013..>995830)
/gene="RRP5"
/locus_tag="AWJ20_4019"
/db_xref="GeneID:30036105"
mRNA complement(join(<994013..995530,995642..>995830))
/gene="RRP5"
/locus_tag="AWJ20_4019"
/product="Rrp5p"
/transcript_id="XM_018881067.1"
/db_xref="GeneID:30036105"
CDS complement(join(994013..995530,995642..995830))
/gene="RRP5"
/locus_tag="AWJ20_4019"
/inference="similar to AA sequence:KEGG_Orthology:K14792"
/note="RNA binding protein involved in synthesis of both
18S and 5.8S rRNAs; component of both the ribosomal small
subunit (SSU) processosome and the 90S preribosome; acts
as part of a Mak21p-Noc2p-Rrp5p module that associates
with nascent pre-rRNA during transcription and has a role
in bigenesis of the large ribosomal subunit; has binding
preference for single stranded tracts of U's; relocalizes
from nucleolus to nucleus upon DNA replication stress;
GO_component: GO:0030686 - 90S preribosome [Evidence IDA]
[PMID 12150911]; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 22842922]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 8896463]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0032040 - small-subunit processome
[Evidence IDA] [PMID 12068309]; GO_function: GO:0003723 -
RNA binding [Evidence IEA]; GO_function: GO:0003729 - mRNA
binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0008266 - poly(U) RNA binding [Evidence IDA] [PMID
16428605]; GO_process: GO:0006396 - RNA processing
[Evidence IEA]; GO_process: GO:0000480 - endonucleolytic
cleavage in 5'-ETS of tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
8896463]; GO_process: GO:0000447 - endonucleolytic
cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and
LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 8896463]; GO_process:
GO:0000464 - endonucleolytic cleavage in ITS1 upstream of
5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID 8896463];
GO_process: GO:0000472 - endonucleolytic cleavage to
generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 8896463]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA]; GO_process:
GO:0042254 - ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Rrp5p"
/protein_id="XP_018733694.1"
/db_xref="GeneID:30036105"
/translation="MSFKRKRDEESAGVAPVSQPPSALRMEDTAFPRGGASTVLTPLE
IKQATNEATRDLFTVRFYWASESNAANGKKKSKKQHRNKVHQKSEAGSDKAGEKHKIK
VESLSFGKLTAGSVVLGQVVQINTLDIALSLPNNLLGYVPITNISAKLSERISKAEDD
DESGSEDEDNEDSDLPQLSQLFKVGQWLRALVVENEKVNNKKRIELSIDPLQVNEEIE
TDDLVAGIAVQGSVQSVEDHGIIVDIGRDDVSGFLSNKELSHAGIEASSLGVGQVLLL
TILSKSANGRTVTLTASATAKKMTIVKAVTLNKSLLAGTLVEVLITDIRSNGLVGKVF
GSIDATIDIVHSGIYDRAVLEQNFKEGKVVKGRVIYSPPAAVSESDDENSTKVFVSLL
ETTIELKVAEDGDALPIGQILEAKVKIVDANGLYVDIGTKSLGFVHVSRVADKRIEDL
SIEEAYSVGTTHPARILNYNYADNLYIVSTEKSVIEQKYLRVEDVSVGEKVTGTVDRI
LPKGGVLIKLWEGFSGIANDIHLSDIKLAHPEKKFKPGMKVTARVSSIICFKVDCGYL
RY"
gene <996712..>997326
/gene="MXR1"
/locus_tag="AWJ20_4020"
/db_xref="GeneID:30036107"
mRNA <996712..>997326
/gene="MXR1"
/locus_tag="AWJ20_4020"
/product="Mxr1p"
/transcript_id="XM_018881069.1"
/db_xref="GeneID:30036107"
CDS 996712..997326
/gene="MXR1"
/locus_tag="AWJ20_4020"
/inference="similar to AA sequence:KEGG_Orthology:K07304"
/note="Methionine-S-sulfoxide reductase; involved in the
response to oxidative stress; protects iron-sulfur
clusters from oxidative inactivation along with MXR2;
involved in the regulation of lifespan; reduced activity
of human homolog implicated in Alzheimer disease;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 20799725]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_function:
GO:0016671 - oxidoreductase activity, acting on a sulfur
group of donors, disulfide as acceptor [Evidence IEA];
GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
reductase activity [Evidence IEA,IEA]; GO_function:
GO:0008113 - peptide-methionine (S)-S-oxide reductase
activity [Evidence IDA,IMP] [PMID 15141092]; GO_function:
GO:0008113 - peptide-methionine (S)-S-oxide reductase
activity [Evidence IDA,IMP,ISS] [PMID 9275166];
GO_process: GO:0034599 - cellular response to oxidative
stress [Evidence IGI,IMP] [PMID 11929995]; GO_process:
GO:0034599 - cellular response to oxidative stress
[Evidence IMP] [PMID 9275166]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0030091 - protein repair [Evidence IEA];
GO_process: GO:0006979 - response to oxidative stress
[Evidence IEA]"
/codon_start=1
/product="Mxr1p"
/protein_id="XP_018733695.1"
/db_xref="GeneID:30036107"
/translation="MASGNFFPKLLVRLLSSSSPKMSATKISPTLDTVGYQVATVGAG
CFWGVEHIYRKHFGNGKGLIDARVGYSGGTTTNPSYKQVCTSGTGHAEALQITFDPSL
VTYDTLIDFFFRMHDPTQLNRQGPDQGTQYRSVIFTHDDEQQKVAEEVKERMQKTFYK
DPIVTKIEPIEAFWDAETYHQLYLHKNPTGYECPSHYVRTKPQL"
gene <999749..>1000363
/locus_tag="AWJ20_4021"
/db_xref="GeneID:30036108"
mRNA <999749..>1000363
/locus_tag="AWJ20_4021"
/product="hypothetical protein"
/transcript_id="XM_018881070.1"
/db_xref="GeneID:30036108"
CDS 999749..1000363
/locus_tag="AWJ20_4021"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733696.1"
/db_xref="GeneID:30036108"
/translation="MAVVGISGYAVYAFDVLVAHLEGRAPVTLAQCLAAVPGSPPLTG
KSPLFVTWNTIAKDGDASLRGCIGTFQEEELESGVASYSLTAALEDPRFRPIQKRELP
HLECGVTILKNFESAANPMDWEIGVHGIKGVFTHRGRRTSATFLPDVAVEQGWDKETT
LVYLADKANAHPDSTISLTRYQGEKSAIRYEEYLDILKQIDARE"
gene complement(<1001193..>1002584)
/locus_tag="AWJ20_4022"
/db_xref="GeneID:30036109"
mRNA complement(<1001193..>1002584)
/locus_tag="AWJ20_4022"
/product="hypothetical protein"
/transcript_id="XM_018881071.1"
/db_xref="GeneID:30036109"
CDS complement(1001193..1002584)
/locus_tag="AWJ20_4022"
/inference="similar to AA sequence:KEGG_Orthology:K01443"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733697.1"
/db_xref="GeneID:30036109"
/translation="MPRISTSTPNTSGYVDSDADDSLVSPNSPSSVFSQEDKFLTGVT
APIIRFINATLCEEGKSYPNSQLWVDAESGVILKSSIDTEFSDDQEPSPEEVTTIDLN
GDILSPGFIDAQLNGAFGMDFCDVNYGDGSIKAFEEGVAYIGRQLFKTGVTSYCPTLP
STYPHVYQKVLPSLKPKRISTAAESLGYHLEGPFLSPKKPGCHPRDAIVTAPKHFQSF
EEIYGADNIDGAAIITLAAEQEGVMESIPDLTKRGITVSLGHSVLDYDGGCKAVNLGA
KMITHVYNAMTQPHHREPGLFGLLGAPNKDLPDSSCQLTKPYYGIIVDGVHVHRSGVA
VAYHAHPEGCCLVTDAMFPMGLPSGTYTWGEQTIVKDDLLLFLNGTNTIAGSGVELDT
CLRNLMAWAEIPIETALKTVTTHPARVLGVENKKGTLRAKADADLTILDAAGNIKHVY
KLGQCVYRAADKK"
gene <1004474..>1007383
/locus_tag="AWJ20_4023"
/db_xref="GeneID:30036110"
mRNA <1004474..>1007383
/locus_tag="AWJ20_4023"
/product="hypothetical protein"
/transcript_id="XM_018881072.1"
/db_xref="GeneID:30036110"
CDS 1004474..1007383
/locus_tag="AWJ20_4023"
/inference="similar to AA sequence:KEGG_Orthology:K01207"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733698.1"
/db_xref="GeneID:30036110"
/translation="MDFEFSDQLPVQLTELGKAIGQMFLVGFAEDYVTDETRELITKY
HIGSIMLRAKNLRDAETTRNLILELQQIAHDAGHEHPLLIAIDQENGMLNNLNDEEYL
SQFPGPMAIVATKSLNLARQVAHATGQQLKSLGVNWLLGPVLDVLTNSTNRLLGVRTM
GDDAQEVTRFSLAFMEGYESAGICQCGKHFPGYGNATVDSTVSLPVVPESIEQLETSS
LIPFREAIKANIDAIMVGGCALPKIAMNEMHACLSERVVRELLREDMGYDGVIVSECL
EMDALYENVGVRQGAVMTAIAGCDVVLVCSSYRLQIEAISGIFGAVTSGILEHEAIYN
SAKRVAKLKSKHLSWQEALNPKPLSYLKELKKTHLQLSKTAYQQSITLVRDHSKCIPL
SESIERDSDIVLLTPLVTPLVSTKHDANLDHLLLGENVFQEFGRSLARLHTGKVLHAS
YTANGVISMHDQLIERAQAVIIVTTDAHRNMYQVGFTKHVGIICAQQRKPVIAIAASS
PYDLALDRAIGTYLCLYEFTNEALDTTAKVLFGQLPARGHFPGSGLYQRTQNRPTTNG
NRTNRNKWLVEKWSHERDMKRLKTLWENCFSDRHLGPGVGGAFQLLLTSGQTHFIVRN
SSTNNLYGFCATWVYEKQGIGCIAMIFVEPSRRGMSIGRSLHDRAVKYLIRERNVSSV
KIGCSIPSFFHGIPTNNYGPLMKWFQEAGWVIDVNLAQVLAADSSDTDPILPQQTFNS
TYPQPTNSISHPHSGHSALHLTDGSIDLGDTVSNPTDGSTITVLSTMLLTSLSEWSPS
KTLIRQLEVLGIKFDICLNEDRFSEIMAFANSPKYAQFEGLTELYREALSSGDSKIIV
ALDPVKKTVVGSIILFTRGSSIASYMPWIVEFEDARVGGLCGVITDPQYNKELESVFN
CGLVGCGVRQFKLQGFERCVIDRATGHQAEGYAENGFYTLRRYLQVSNVAQSFVV"
gene <1009856..>1011643
/gene="HXK2"
/locus_tag="AWJ20_4024"
/db_xref="GeneID:30036111"
mRNA <1009856..>1011643
/gene="HXK2"
/locus_tag="AWJ20_4024"
/product="hexokinase 2"
/transcript_id="XM_018881073.1"
/db_xref="GeneID:30036111"
CDS 1009856..1011643
/gene="HXK2"
/locus_tag="AWJ20_4024"
/inference="similar to AA sequence:KEGG_Orthology:K00844"
/note="Hexokinase isoenzyme 2; catalyzes phosphorylation
of glucose in the cytosol; predominant hexokinase during
growth on glucose; functions in the nucleus to repress
expression of HXK1 and GLK1 and to induce expression of
its own gene; antiapoptotic;
phosphorylation/dephosphorylation at serine-14 by protein
kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates
nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog,
HXK1, that arose from the whole genome duplication;
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
11311123]; GO_component: GO:0005829 - cytosol [Evidence
IDA] [PMID 9563516]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16962558];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11311123]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 9563516]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0004396 -
hexokinase activity [Evidence IEA,IEA]; GO_function:
GO:0004396 - hexokinase activity [Evidence IDA] [PMID
12611889]; GO_function: GO:0004396 - hexokinase activity
[Evidence IDA] [PMID 332086]; GO_function: GO:0004396 -
hexokinase activity [Evidence IMP] [PMID 6341351];
GO_function: GO:0016301 - kinase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0016773 - phosphotransferase
activity, alcohol group as acceptor [Evidence IEA];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_process: GO:0005975 - carbohydrate metabolic
process [Evidence IEA]; GO_process: GO:0046835 -
carbohydrate phosphorylation [Evidence IEA,IEA];
GO_process: GO:0032445 - fructose import [Evidence IGI]
[PMID 6300872]; GO_process: GO:0006000 - fructose
metabolic process [Evidence IMP] [PMID 6300872];
GO_process: GO:0046323 - glucose import [Evidence IGI]
[PMID 6300872]; GO_process: GO:0006006 - glucose metabolic
process [Evidence IMP] [PMID 6300872]; GO_process:
GO:0006096 - glycolytic process [Evidence IEA,IEA];
GO_process: GO:0006096 - glycolytic process [Evidence IDA]
[PMID 332086]; GO_process: GO:0006096 - glycolytic process
[Evidence IDA,IMP] [PMID 8917466]; GO_process: GO:0019318
- hexose metabolic process [Evidence IEA]; GO_process:
GO:0006013 - mannose metabolic process [Evidence IDA]
[PMID 332086]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0016310 - phosphorylation
[Evidence IEA]; GO_process: GO:0008361 - regulation of
cell size [Evidence IMP] [PMID 12089449]; GO_process:
GO:0046015 - regulation of transcription by glucose
[Evidence IDA] [PMID 11311123]; GO_process: GO:0001302 -
replicative cell aging [Evidence IMP] [PMID 15722108]"
/codon_start=1
/product="hexokinase 2"
/protein_id="XP_018733699.1"
/db_xref="GeneID:30036111"
/translation="MNTIVPLSTRGPSSPEGSITASTTSTTPSSSPPSSSKSAPSSAG
FLSPPASPQPEKHAASGIMGSSTVSTSTTTARTSSRTATKSNLISGTVLDGLNDNLHR
LVTPALSPTSSDRNNDENVHEIDHEIDISLPTPVSSTGDHFKLFASSYDLLSLDAVAK
RTTSHLTRVLGTNHHTPTMIPTCMTCLPSGKEIGTFLSVDLGGSTFRVAFVCLKGPGK
QSEIVYMSVVPVPDSVKQGSGNDFFLWMAHEVKKGVDKVLPNQPKSVPINMGISWSFP
FVQTAMHTAIIEKMGKGYNVADEISGWNLGKSFENAFEKLDLNIKVTAIVNDGGACMI
SRAYADNATKISLILGTGINAGIMVPSHLIGEAKLESIASGKPAPTYMINTEISMLDD
TIVPASIWDVELDAAIERPGFQPLETKVSGRYLGEISRLIIRDLVLNNTLCGGILPEK
LDVPYSLESSLMSDIEEHFRNSRLDLARDAFLRAHPHPYLSDSDLDQICDVFVNVSNR
SAAFTAAVLVGLADIIYQNTAHSSAESQSCIISYTGTVIEKYPLFRKRCQQFLDSLCA
SRGIQLRLESSENGSIFGPVIAAAMNHCN"
gene complement(<1013454..>1015280)
/gene="MSS4"
/locus_tag="AWJ20_4025"
/db_xref="GeneID:30036112"
mRNA complement(<1013454..>1015280)
/gene="MSS4"
/locus_tag="AWJ20_4025"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881074.1"
/db_xref="GeneID:30036112"
CDS complement(1013454..1015280)
/gene="MSS4"
/locus_tag="AWJ20_4025"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733700.1"
/db_xref="GeneID:30036112"
/translation="MGAFFLTNGDYRPSNGTHKVPRHPVLSDVHCGVSVPKYGACAQL
ENRRPARRSAFAYHKVLILECSPDDSGVSVGSDETLNEPTVWKPIDIVSEQAYCNSAP
IDNDDDNSSEWSFETEDEAAFTTRQIETASSKEDTEATWSAPAASWALSDCPIEIDEN
DFTPESHGEVTLAESPLETSHSDLAESEVITPAKVDEQTTWPTADAPAPTIEGSCSID
SLVAEIIAVANLFKDLPQLIVSGGLQAGPESELPKTAPLALSKPIAQTPSDDLVIQVE
EVDKSKTPLLLTRAKAKLTSFIGHAAPISKSTLMFNGVQTATYATPLSSQEALSMENK
KYRLDGGAIIKVYSPTVYQNIRTLCGIDYHEFLNSFVLQSDLTKTKSPGKSGSDFLFT
PDGKYIIKTIKRKEHNVIANADFLADYYNHVKAHPGTQLPFYLGNYTLVAGGKKTHFV
IMKNLLQKETDLIYDLKGSSHDRRAGPRKDNRGRVVFKDLDWTDKHEAISMSQEDRDK
LLVQVSKDVGLLKRHNIMDYSLLVGFQSDTRTCEQQPVIGMIDTLCPFSWRKRAETTA
KGLFFGRSAVDVVHPQKYGARFLNFVQSAVVPGPGRSDSTRC"
gene complement(<1030228..>1031409)
/locus_tag="AWJ20_4026"
/db_xref="GeneID:30036113"
mRNA complement(<1030228..>1031409)
/locus_tag="AWJ20_4026"
/product="homogentisate 1,2-dioxygenase"
/transcript_id="XM_018881075.1"
/db_xref="GeneID:30036113"
CDS complement(1030228..1031409)
/locus_tag="AWJ20_4026"
/inference="similar to AA sequence:KEGG_Orthology:K00451"
/codon_start=1
/product="homogentisate 1,2-dioxygenase"
/protein_id="XP_018733701.1"
/db_xref="GeneID:30036113"
/translation="MRPAVAHKGYTDLPDKPDMESVFLSTNPKVHVSPTQLAWSPFDI
PTEKTTFVDGLKTVAGSGEPSLREGLATHVYTANASMKNQAFVNQDGEFLIMPQQGRV
DVQTEFGWLYVQPGEFLVVQRGIRFSVNLPDGPSRGYILEIWGSNFELPDLGPLGGYG
MANARDFLYPQASYDLEPVSNFEIITKLNGKFFKSTQDHTPYDVVAWHGNYVPYKYDM
TKFVNIGSISVDHIDPSVFTIMTAKSRDPNAALADVMIFMPRWDVASHTYRPPYYHRN
VASELMGLIYGDYGGRSDSFQPGSVSYETGMTPHGVAYPEFKAASENPPPTQQISLGS
MAFMFESSRPFTITDYAWKSDKIHFHETGMWDDLVNNFTLHLEEVKKLTNGKHEHGVN
H"
gene <1032590..>1033435
/locus_tag="AWJ20_4027"
/db_xref="GeneID:30036114"
mRNA <1032590..>1033435
/locus_tag="AWJ20_4027"
/product="Mediator of RNA polymerase II transcription
subunit 18"
/transcript_id="XM_018881076.1"
/db_xref="GeneID:30036114"
CDS 1032590..1033435
/locus_tag="AWJ20_4027"
/inference="similar to AA sequence:KEGG_Orthology:K15136"
/codon_start=1
/product="Mediator of RNA polymerase II transcription
subunit 18"
/protein_id="XP_018733702.1"
/db_xref="GeneID:30036114"
/translation="MMYQQLSLVGSLKDAEIKITLHTLAALSGMKPTRIFNHHLVWIP
TYPFNPVLAAGEVNQIEQYRITCDTSLVDPASEDEYLPPAGFKENKDFLQTMPWKMII
SEVPEAGKRQVTSQSILESAIVEGSPFEFMEKMGYKFSHEYWSKGSEFVFGNIVVRIF
RICVKDQITAPTTEDQNLADTTEGSGTADNAMVIDDDSFGDKITAETALASQKAAYQP
LKLLDKSGQWSIKAFLSVKQLTDLESIITGTAQLEKFQAEMAGLFELKMADRNSYDTR
LRANR"
gene complement(<1033650..>1035482)
/gene="NOP7"
/locus_tag="AWJ20_4028"
/db_xref="GeneID:30036115"
mRNA complement(<1033650..>1035482)
/gene="NOP7"
/locus_tag="AWJ20_4028"
/product="Nop7p"
/transcript_id="XM_018881077.1"
/db_xref="GeneID:30036115"
CDS complement(1033650..1035482)
/gene="NOP7"
/locus_tag="AWJ20_4028"
/inference="similar to AA sequence:KEGG_Orthology:K14843"
/note="Component of several different pre-ribosomal
particles; forms a complex with Ytm1p and Erb1p that is
required for maturation of the large ribosomal subunit;
required for exit from G0 and the initiation of cell
proliferation; GO_component: GO:0070545 - PeBoW complex
[Evidence IDA,IPI] [PMID 16287855]; GO_component:
GO:0005730 - nucleolus [Evidence IEA,IEA,IEA];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
10684247]; GO_component: GO:0005654 - nucleoplasm
[Evidence IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 10684247]; GO_component: GO:0030687 -
preribosome, large subunit precursor [Evidence IEA];
GO_component: GO:0030687 - preribosome, large subunit
precursor [Evidence IDA] [PMID 11583614]; GO_component:
GO:0030687 - preribosome, large subunit precursor
[Evidence IDA] [PMID 23212245]; GO_function: GO:0003729 -
mRNA binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0043021 - ribonucleoprotein complex binding [Evidence
IEA]; GO_process: GO:0044786 - cell cycle DNA replication
[Evidence IMP] [PMID 12110181]; GO_process: GO:0000466 -
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IEA];
GO_process: GO:0000463 - maturation of LSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IEA]; GO_process: GO:0000462 -
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
12837249]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042273 - ribosomal large
subunit biogenesis [Evidence IEA]; GO_process: GO:0042273
- ribosomal large subunit biogenesis [Evidence IDA] [PMID
11911362]; GO_process: GO:0042273 - ribosomal large
subunit biogenesis [Evidence IDA] [PMID 16888624];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA,IEA]"
/codon_start=1
/product="Nop7p"
/protein_id="XP_018733703.1"
/db_xref="GeneID:30036115"
/translation="MRIKKKHQAGAAKNFITRTQAVKKLQVSLADFRRLCIFKGIYPR
EPRNKKKANKGSTKPTTFYYTKDIQYLLHEPVLNKFREHKIFARKLSRALGRGEVGDA
KRLDDNRPRYKLDHIIKERYPTFNDALRDLDDPLCMLFLFAAMPATNSVNYKVIEDAD
RLCNQWMAYVARERLLRKVFVSIKGVYYQARVKGQDILWLVPFKFPQNIPDDIDFRIM
LTFLEFYTTLLQFVLYRLYTESGLVYPPQINASKMKGVGGISAYLLESRQNGLSLLGA
KASENTNGKATTNGATPNLKMDRIAQADEEADHDGDEQEEDENDAANESALDTFQPIP
ATETAASDILAQPAPESEREGQLFSNFTFFVGREVSLDIVEFLIIAFDGKVISESAID
ELIDNENEESSNDATEAYKKIDLSTVTHQICDRPTVPNKVPGRTYIQPQWIFDSINKG
QLLPVANYAPGEALPPHLSPWGDAANYDPEAALEDGEGESSSEAEEEETIEGEALPDE
DEEEVDEEAEEALERQRDLEKEAAGIKFSEQQSSAESKKRKKKTAADKEQEEKDLRKI
MMSNKQKKLYNKMQYGIDKKQAREEELKKKRRKIEATKEKLKSS"
gene complement(<1038269..>1039882)
/gene="STL1"
/locus_tag="AWJ20_4029"
/db_xref="GeneID:30036116"
mRNA complement(<1038269..>1039882)
/gene="STL1"
/locus_tag="AWJ20_4029"
/product="glucose-inactivated glycerol proton symporter
STL1"
/transcript_id="XM_018881078.1"
/db_xref="GeneID:30036116"
CDS complement(1038269..1039882)
/gene="STL1"
/locus_tag="AWJ20_4029"
/codon_start=1
/product="glucose-inactivated glycerol proton symporter
STL1"
/protein_id="XP_018733704.1"
/db_xref="GeneID:30036116"
/translation="MVDISGGIRSRTNTGGLTGRRLRWAITLVATIGFSLFGYDQGLM
SGQITAPQFNKEFPATAGTSSHATVIQGAVTSCYELGCFFGAMFALFKGEKIGRRPLI
LLGSSIIILGTVISVTAFGPHWGLGQFVVGRVITGLGNGINTSQIPIWQSEISKAENR
GRLVNLEGSVIAVGTFIAYWLDYGFSFTNTSVQWRFPVAFQIFFAGLLFFGMWSLPES
PRWLISQGRHEEAISILAALNNTDINDEQIIAEARVVMEVVSRNKDQASIREVFTGGK
TQHFRRMLIGSSTQFFQQFTGCNSSIYYSTVLMKTSMNVSDSLALILGGVFATVYALS
TIPSFFLIDSIGRRKLFIIGALGQGISFTITFACLIPNTMEASKGAIVGLYLFIFFFA
FTILPLPWVYPPEINPTRTRTVATGFSTCTNWLSNFVIVMITPIFVTNTRWGCYLFFA
LINFLFVPIIYFFYPETAGRSLEEMDIIFAKGYVEKKPYFRVAQQLPSLTPEQVENEA
ISLGLYEAPSEKGDADYVDDITNSETTSS"
gene complement(<1042292..>1043908)
/gene="SGA1"
/locus_tag="AWJ20_4030"
/db_xref="GeneID:30036118"
mRNA complement(<1042292..>1043908)
/gene="SGA1"
/locus_tag="AWJ20_4030"
/product="glucan 1,4-alpha-glucosidase"
/transcript_id="XM_018881080.1"
/db_xref="GeneID:30036118"
CDS complement(1042292..1043908)
/gene="SGA1"
/locus_tag="AWJ20_4030"
/inference="similar to AA sequence:KEGG_Orthology:K01178"
/note="Intracellular sporulation-specific glucoamylase;
involved in glycogen degradation; induced during
starvation of a/a diploids late in sporulation, but
dispensable for sporulation; GO_component: GO:0000324 -
fungal-type vacuole [Evidence IDA] [PMID 2493265];
GO_component: GO:0005628 - prospore membrane [Evidence
IDA] [PMID 24390141]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0004339 - glucan
1,4-alpha-glucosidase activity [Evidence IEA,IEA];
GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
activity [Evidence IDA] [PMID 2493265]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0016798 - hydrolase activity, acting on
glycosyl bonds [Evidence IEA]; GO_function: GO:0004553 -
hydrolase activity, hydrolyzing O-glycosyl compounds
[Evidence IEA]; GO_process: GO:0030437 - ascospore
formation [Evidence IEP] [PMID 350852]; GO_process:
GO:0005975 - carbohydrate metabolic process [Evidence
IEA]; GO_process: GO:0005980 - glycogen catabolic process
[Evidence IMP] [PMID 11486014]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0000272 -
polysaccharide catabolic process [Evidence IEA];
GO_process: GO:0005976 - polysaccharide metabolic process
[Evidence IEA]; GO_process: GO:0030435 - sporulation
resulting in formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="glucan 1,4-alpha-glucosidase"
/protein_id="XP_018733705.1"
/db_xref="GeneID:30036118"
/translation="MLVEKFLILLASCSVTSSLPIYESLSSFVGFTSPDEQKLLNGVD
ATRSSSSKGGHRVPGSLINEIEYEDWLKTETDLAFDRVISNIGGYGQGLDDVLPGAVI
ASPSKSKPNYYYIWTRDAAISINSLITRYDDQKGQNETLRSIINDYLNSSAIMQHVDN
PSGTFENLEGLGEPKFMVDGRPFTSTWGRPQRDGPPLRASTMINYVNSEVRNNNLQYK
DFEDLYYNVIRPDLDYVVQHWKDKSFDLWEEVYGNHFFTSMVQLKSLIHGAEIAHELG
DKISFYVFRGVASELREYIRQYWDSSRKHLVETLDSKERSGLDTALFLGSIHAIDLYN
WDGIDTDELVFPPYSDEVIASIDHLVNDMRYRFPINARRLDEFSKQGKNNSLAGVGIG
RYPEDVYDGADVTTGNPWFLCTATVSQTLYLLADHLITRPDSYKLVISDITKPFYSLF
LSDNENFDSPDFSIARTNEAYTALVKRIIEYADSFLDVIREHHANDGSMSEQFSRYDG
YMRGATHLTWSYSAFWAASRQRNLTLSRLY"
gene <1046236..>1049613
/gene="ARK1"
/locus_tag="AWJ20_4031"
/db_xref="GeneID:30036119"
mRNA <1046236..>1049613
/gene="ARK1"
/locus_tag="AWJ20_4031"
/product="serine/threonine protein kinase ARK1"
/transcript_id="XM_018881081.1"
/db_xref="GeneID:30036119"
CDS 1046236..1049613
/gene="ARK1"
/locus_tag="AWJ20_4031"
/inference="similar to AA sequence:KEGG_Orthology:K08853"
/codon_start=1
/product="serine/threonine protein kinase ARK1"
/protein_id="XP_018733706.1"
/db_xref="GeneID:30036119"
/translation="MQKQAPPGAYPPGTQLTVGSHAVTIKSYISEGGFAHVYVVKISP
STLEGEIACLKRVVVPDKIHLNLLRAEVDAMKRLKGHPNIVRYIDSHAARLNAQGGLP
ASAQAQLQAQGHGQGGSQVYEVFLLMEYCSGNGLIDFMNTRLREQLTESEILKITSDV
TYGLACMHYLQPPLIHRDLKIENILISGDGTYKLCDFGSVSPILRPPRNPGEYHILEQ
DIQRHTTAQYRSPEMIDIYRGHPIDEKSDIWALGVFLYKLCYYTTPFEREGSLAILHA
SFSFPPKPVYSDRLKGVISALLREDPRARPNVYQVLKEICAMRGLEVPIKDIYSGATS
TAATMVQPTTSVQLAHPAQDNQNHSLSASQQQPDNVSTAQGIVRSIPKEQQQRQWVPE
VTPMFRGRITVQQNNSMPSLDKFDNGDENHESRGSFTTGLASAANFDDDPFSSLDSSA
KQQRAKTQPVTPVHSSTPQEFTFDEADIESRFPSVEDLARELERTPFEFAQAHKPIQQ
QTNLQARTQPQVQKQTQTAPQPQFKPQMQSQLRSQPEGQVSQEMPRQVQSQQAIPQPA
TRLEQSTTSVSRSSRDSTPGEPLKTKPPPTMRKPGTTGTPSGYSAYPLKSASPPMPAP
KKLSQTSQPKPEKPALNTMPSGLSINISSASSGSDSEPTPSESYSKPLPRPPPHQIRP
RPLSMYSQSNTDAYFEASYNSVNQSPSPVIDQSQKFSYLPEDSNAIANTGNANHTGSL
IDTMPPEDREQLKVILTGLSQRSNTVVLDNSENHIDSNVDFLKALDNENTGSRPHHHR
KPSQSQYLYPSGTGDGSGGVGNGGSTGGSGWMNRSSSTSRRSGSTSRHSKKPSMTSLK
GKIGDAFKKFDNAAGAYRSTSDSSMMGPPVAAPRTVSAGSNPGGYRSSSRMSYTAESP
GDYAFSPKKEDDEFKYPVSSSTSVKHGPPHVHMHRSKSRNNGSSSIQSRVQQFLSRSE
SPPKKTASGYGKYTEEERQVSSSTHSPSPPNDTSSMPPPPPSKQYAAQKLGREVHREI
KRASMESTRDWINSDGSGRRNSKMHLPGRTDSQHSRKLSNISSESSGSNSGSKRPPVR
PKKPAHLQSPRRTVDKVQKAIDDDWEASFNQKYPSLG"
gene <1055708..>1056874
/gene="PCP1"
/locus_tag="AWJ20_4032"
/db_xref="GeneID:30036120"
mRNA <1055708..>1056874
/gene="PCP1"
/locus_tag="AWJ20_4032"
/product="Pcp1p"
/transcript_id="XM_018881082.1"
/db_xref="GeneID:30036120"
CDS 1055708..1056874
/gene="PCP1"
/locus_tag="AWJ20_4032"
/inference="similar to AA sequence:KEGG_Orthology:K09650"
/note="Mitochondrial serine protease; required for the
processing of various mitochondrial proteins and
maintenance of mitochondrial DNA and morphology; belongs
to the rhomboid-GlpG superfamily of intramembrane
peptidases; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IEA,IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IDA] [PMID
12774122]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0008233 -
peptidase activity [Evidence IEA]; GO_function: GO:0004252
- serine-type endopeptidase activity [Evidence IEA];
GO_function: GO:0004252 - serine-type endopeptidase
activity [Evidence IMP] [PMID 12774122]; GO_function:
GO:0004252 - serine-type endopeptidase activity [Evidence
IMP] [PMID 20558178]; GO_function: GO:0008236 -
serine-type peptidase activity [Evidence IEA]; GO_process:
GO:0006508 - proteolysis [Evidence IEA,IEA]; GO_process:
GO:0010821 - regulation of mitochondrion organization
[Evidence IMP] [PMID 11907266]; GO_process: GO:0010821 -
regulation of mitochondrion organization [Evidence IMP]
[PMID 12707284]; GO_process: GO:0006465 - signal peptide
processing [Evidence IMP] [PMID 12417197]"
/codon_start=1
/product="Pcp1p"
/protein_id="XP_018733707.1"
/db_xref="GeneID:30036120"
/translation="MRNLSSNLSRSAFHVSGQLFGISRQKAFSSEAIGQVLIRGVAAR
HYSRNVTSSFSTLGSKYEAFTRSHTSINTPEKSVSVKHFTAGFFTNTFHTQNGPFLQS
FTKSVSPRQSILFMATHRDHGYGGSNGGSSYGRGGNWGKFNRYKNILVNHRVPLLFTG
AVLTVTTFIFPVLFQMPGMGVVKQNPQLVVYALIGLNVAGFLAWKTKQGSRFMYRYGL
LHKDSQFNKWSMIGSAFSHQEFWHIGINMYVLYNFGTTVASWVGTEQFLAAYIDGAVI
SSLASLMLPVLVRRFQNIPSLGASGAVFTTFGLFSYLAPHAKLALMFIPLPFGAWTVF
LGTMAYNGAGLFMRFGLSDYAGHLGGSIVGIIWGYYLTQRARRIRQRRSVINYR"
gene complement(<1069797..>1072009)
/gene="MSS4"
/locus_tag="AWJ20_4033"
/db_xref="GeneID:30036121"
mRNA complement(join(<1069797..1071562,1071847..>1072009))
/gene="MSS4"
/locus_tag="AWJ20_4033"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881083.1"
/db_xref="GeneID:30036121"
CDS complement(join(1069797..1071562,1071847..1072009))
/gene="MSS4"
/locus_tag="AWJ20_4033"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733708.1"
/db_xref="GeneID:30036121"
/translation="MLALDTGLFPVSTPAGSPAGVPAAAGCRLQVAATGYVNQDYVVG
ADINIIFRAPCSHRVPRRPVLSDVNHGIVVPKYGACAQQENRRLARRSAFAYHKVLIL
ECSPDDSGVSVGSDETLNEPTVWKPIDIVAEQAYCSSAPIDNDDDNSSEWSFETGDEA
AFTTGQFGTTWSEEDTEATWSAPAASWALSDCPIEIDENESTPESHGEVTLVESPLET
SRSDLAESEEITPSKVAEVPCAIADIPTSNSSIDSLVAEIIAVATLYKGLPQLVVSGG
LQADSEFELPKTAPLALSKPIAQTPSDDLVIQVEEVDKSKTTLLLTRAKAKLTSFTGH
ASPILKSTLMFNGVQTAVYATPLSSHETLTMENKKYRLDGGAIIKAYSPIVYQSIRTL
CGIDCHEFLNSFVLQSDLTKTKSPGKSGSDFLFTPDGKYIIKTIKRKEHNVIANSEFL
ADYYNHVKAQSSTQLPFYLGNYTLVAGGKKTHFVIMKNLLQKETQLIYDLKGSTHDRR
AGPRKDNRGRVVFKDLDWTDKHEAIFMSQEDRDKLMVQVSKDVDFLKQHNIMDYSLLV
GLQSDTRTCQQQPVIGMIDTLCPFSWRKRAETISKSLIFGSSAIDVVHPSKYGARFLN
FVQSAVVPRPGRSVSTRC"
gene complement(<1073340..>1074926)
/gene="MSS4"
/locus_tag="AWJ20_4034"
/db_xref="GeneID:30036122"
mRNA complement(<1073340..>1074926)
/gene="MSS4"
/locus_tag="AWJ20_4034"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881084.1"
/db_xref="GeneID:30036122"
CDS complement(1073340..1074926)
/gene="MSS4"
/locus_tag="AWJ20_4034"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733709.1"
/db_xref="GeneID:30036122"
/translation="MYHYDIPLRRSLANYARPVRYMARNLIKFTDHLYTCSYLLPEVN
IPQLLWDRSVQSKFHSANELAVKYLESKGYCNTYVCEKLCEKKTQELVRKARLSQSSA
WSTLTSAIADLNETIEIARDQIDRSEVELWRAIRGVEKHLNFVLTLGATVSTESIKKA
ESHLSGRLSTWETARAPRPKKRVPSATKSSTNPRDTRGTRPSSSHQQKVSIPTTAKRS
YEKKTPTRSLRTAILFDGVYRCVCTSLTPGVSHISSPTIYAVAGGTIEGHSQDLFNTI
RRLQGIRTSDYLSAFDLNSAGIAIKSPGRSGSDLLYTENGKYIIKTIPSSEHEVLIDL
LPSYVEHLERHKNSLLPVFLGHYTHEDNGKRTHFVVMKNLFSGHGLVDKVFDLKGSTY
NRAVDENTDNVGRLVYKDIEWMKHKETIQMNEEERTKFMIQLMNDIAFLKRNNIFDYS
MLVGKYDDSKKGVMGIIDILTPYNKPQALKRTALGLLHDPTTLSCMKPCDYAARFFNF
MESKVVPAVITGRVSRHHKH"
gene complement(<1078022..>1078540)
/gene="UBC6"
/locus_tag="AWJ20_4035"
/db_xref="GeneID:30036123"
mRNA complement(<1078022..>1078540)
/gene="UBC6"
/locus_tag="AWJ20_4035"
/product="E2 ubiquitin-conjugating protein UBC6"
/transcript_id="XM_018881085.1"
/db_xref="GeneID:30036123"
CDS complement(1078022..1078540)
/gene="UBC6"
/locus_tag="AWJ20_4035"
/inference="similar to AA sequence:KEGG_Orthology:K04554"
/note="Ubiquitin-conjugating enzyme involved in ERAD;
located at the cytosolic side of the ER membrane; tail
region contains a transmembrane segment at the C-terminus;
substrate of the ubiquitin-proteasome pathway;
ER-associated protein degradation is also known as ERAD;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IDA] [PMID
8396728]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0016881 -
acid-amino acid ligase activity [Evidence IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0004842 - ubiquitin-protein
transferase activity [Evidence IEA]; GO_function:
GO:0004842 - ubiquitin-protein transferase activity
[Evidence IMP] [PMID 18191224]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence IDA,IMP]
[PMID 8396728]; GO_process: GO:0030433 - ER-associated
ubiquitin-dependent protein catabolic process [Evidence
IMP] [PMID 18191224]; GO_process: GO:0030433 -
ER-associated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 8393731]; GO_process:
GO:0030433 - ER-associated ubiquitin-dependent protein
catabolic process [Evidence IGI] [PMID 8396728];
GO_process: GO:0030433 - ER-associated ubiquitin-dependent
protein catabolic process [Evidence IGI,IMP] [PMID
8641272]; GO_process: GO:0030433 - ER-associated
ubiquitin-dependent protein catabolic process [Evidence
IGI,IMP] [PMID 8781238]; GO_process: GO:0006513 - protein
monoubiquitination [Evidence IDA,IMP] [PMID 8396728];
GO_process: GO:0000209 - protein polyubiquitination
[Evidence IMP] [PMID 19345192]; GO_process: GO:0016567 -
protein ubiquitination [Evidence IEA]"
/codon_start=1
/product="E2 ubiquitin-conjugating protein UBC6"
/protein_id="XP_018733710.1"
/db_xref="GeneID:30036123"
/translation="MMTPSGRFKTSTRLCLSISDYHPKTWNPAWSVSTILTGLLSFMV
SDESTAGSMISTDEQKRKLARESLAWNVKYNVNFNIHFPEVAKKNSADLVAQQAQEAL
AASANSTASQKPNGVTQSGTATLVQAGAGQETEKTATTNTSGFSLGQKALCVVVVVAS
YVLASHLIQVYR"
gene complement(<1081739..>1081837)
/locus_tag="AWJ20_4036"
/db_xref="GeneID:30036124"
tRNA complement(<1081739..>1081837)
/locus_tag="AWJ20_4036"
/product="tRNA-Ser"
/db_xref="GeneID:30036124"
gene complement(<1082163..>1083047)
/locus_tag="AWJ20_4037"
/db_xref="GeneID:30036125"
mRNA complement(<1082163..>1083047)
/locus_tag="AWJ20_4037"
/product="U4/U6.U5 small nuclear ribonucleoprotein"
/transcript_id="XM_018881086.1"
/db_xref="GeneID:30036125"
CDS complement(1082163..1083047)
/locus_tag="AWJ20_4037"
/codon_start=1
/product="U4/U6.U5 small nuclear ribonucleoprotein"
/protein_id="XP_018733711.1"
/db_xref="GeneID:30036125"
/translation="MSRQSRASAADFMDYDADSYSADKKDELDSISESRPSDSSKDGD
ENTNDPRSRSREYGRDSDRYGDRSGSRETVRRATRYERDDRDSKRRSERSYSPYRGDS
RRYDRRDDRITDRGDRGDRSGRYERDETPRGRGRYEDSRYDDRYYDPKREIEEDRGDR
RERGRDFGRNRDSYRRYNSARSRSRSRSGEFDSKTKTSNGLSKSRYDSYRDNKAGNDT
DKKQQIDNTPATEAAPEDPEELMRKMMGFSGFSTTKGQKVVGADVSGIAKTAKSSTFR
QYMNRTRGFNRDLSPTRE"
gene <1083338..>1084726
/locus_tag="AWJ20_4038"
/db_xref="GeneID:30036126"
mRNA <1083338..>1084726
/locus_tag="AWJ20_4038"
/product="hypothetical protein"
/transcript_id="XM_018881087.1"
/db_xref="GeneID:30036126"
CDS 1083338..1084726
/locus_tag="AWJ20_4038"
/inference="similar to AA sequence:KEGG_Orthology:K15053"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733712.1"
/db_xref="GeneID:30036126"
/translation="MSLIDTVLANKLFTDRRRVSLYSNFRKLKDSNIDGYEANIAAWK
ILLTDALDQGNFEDATAIPGGPGLLEALYDPDNGRPLALDVVIDELVADKVLIPLPIY
MAEKSSIYSQGAWLTKMLNPKVVTSWVLSYTKVWDSSWKSIGPKAELGTLKKEKYISI
QALETIGNAIWANLENEHGITASYTNGVFTKEMLTSLLSEVSVTKKIHNTVQTSRVLL
SPTDIEVVLKYLVRDKNKLISDGQIYKVNSRNASPLITQKDIAIANLRSTILQVTDRV
NGLSDQIADCDLKARNALERKNKPVAVYALKSKKLAESSQVKALDMLSNLELLVGKID
DATDQAEIVSALSSGAEILTTLNSTIGGAEKVGDLMDQLRDQADETDEIGREISSMVG
RSVDEDEVESELDLMLAEERQKQLDKLPVPPTNSTHATPAKDSQEDPAIASLSDELSA
TSLEPRKKVLAE"
gene complement(<1084955..>1086989)
/gene="UBX7"
/locus_tag="AWJ20_4039"
/db_xref="GeneID:30036127"
mRNA complement(join(<1084955..1086664,1086873..>1086989))
/gene="UBX7"
/locus_tag="AWJ20_4039"
/product="Ubx7p"
/transcript_id="XM_018881088.1"
/db_xref="GeneID:30036127"
CDS complement(join(1084955..1086664,1086873..1086989))
/gene="UBX7"
/locus_tag="AWJ20_4039"
/note="UBX (ubiquitin regulatory X) domain-containing
protein; interacts with Cdc48p; UBX7 has a paralog, UBX6,
that arose from the whole genome duplication;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA,IEA]; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005635 -
nuclear envelope [Evidence IDA] [PMID 14755638];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IMP] [PMID
14755638]; GO_process: GO:0006511 - ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 14755638]"
/codon_start=1
/product="Ubx7p"
/protein_id="XP_018733713.1"
/db_xref="GeneID:30036127"
/translation="MSNSSSSLFRVSFRRFAMGGQAREPASGEFYPKRAREPGAESYI
QTKFVPLEVVAGSQQYVFFSQVFPVAKIPSIFIINQSVVVDRFDSEVDAQEFLDRLAQ
ADQGLVRGVRDVSGNTSGAGNDTGSTANVQNQSSLDSSNGSSLDQNAIAAAAAAAVAA
LTGRSGSASGLPAGSSASVPSVSSVTPSAGSDSSNSTGAGNSTADDSAVTRGSDAATD
RSASTSHNNTISTSGSGSTTTSTNSASNSGANDVSSSSPEPTNPPVPRKEKSSTPTNS
VAASASTNTSNTHSSSTSSSSGLSDTQRYQIQLRKQRQADAEERKRILRLLETDREER
RAQRLHHQQEQQDVPLAAESTTIPQKKKQTRSLSLSQSNQCALVIRLFDGQALKNIFN
NSDTLDIVRKWVDENRTDSEDPYQFYQTFPKRSFGPGEENSTLLELDLCPSSTLILKP
AENVLNAYSSSYSPSAWLQGGTRTISDAIYTFLGIGYKPPPPAPSEPSDEPSRSASPV
PNPRRSGYSTPLPTPRPSDNTNPSSPSPPKSENPAPATLLSANSFSQPRDDVHPSKPN
SISSSRSSSAVNVRTLHDNDDAKDDRLTYNGNQLNLEDDNNS"
gene complement(<1087593..>1089275)
/gene="PDC1"
/locus_tag="AWJ20_4040"
/db_xref="GeneID:30036129"
mRNA complement(<1087593..>1089275)
/gene="PDC1"
/locus_tag="AWJ20_4040"
/product="indolepyruvate decarboxylase 1"
/transcript_id="XM_018881090.1"
/db_xref="GeneID:30036129"
CDS complement(1087593..1089275)
/gene="PDC1"
/locus_tag="AWJ20_4040"
/inference="similar to AA sequence:KEGG_Orthology:K01568"
/note="Major of three pyruvate decarboxylase isozymes; key
enzyme in alcoholic fermentation; decarboxylates pyruvate
to acetaldehyde; involved in amino acid catabolism;
subject to glucose-, ethanol-, and autoregulation;
activated by phosphorylation in response to glucose
levels; N-terminally propionylated in vivo; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
2665820]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 16850348]; GO_function: GO:0047433 -
branched-chain-2-oxoacid decarboxylase activity [Evidence
IMP] [PMID 9546164]; GO_function: GO:0016831 -
carboxy-lyase activity [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA,IEA];
GO_function: GO:0016829 - lyase activity [Evidence IEA];
GO_function: GO:0000287 - magnesium ion binding [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0004737 - pyruvate
decarboxylase activity [Evidence IEA]; GO_function:
GO:0004737 - pyruvate decarboxylase activity [Evidence
IDA] [PMID 23423327]; GO_function: GO:0004737 - pyruvate
decarboxylase activity [Evidence IDA] [PMID 2404950];
GO_function: GO:0030976 - thiamine pyrophosphate binding
[Evidence IEA]; GO_process: GO:0006559 - L-phenylalanine
catabolic process [Evidence IEA]; GO_process: GO:0006559 -
L-phenylalanine catabolic process [Evidence IGI] [PMID
12499363]; GO_process: GO:0000955 - amino acid catabolic
process via Ehrlich pathway [Evidence IEA]; GO_process:
GO:0000949 - aromatic amino acid family catabolic process
to alcohol via Ehrlich pathway [Evidence IGI] [PMID
12499363]; GO_process: GO:0009083 - branched-chain amino
acid catabolic process [Evidence IEA]; GO_process:
GO:0019655 - glucose catabolic process to ethanol
[Evidence IDA] [PMID 2404950]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0006090 -
pyruvate metabolic process [Evidence IDA] [PMID 2404950];
GO_process: GO:0006569 - tryptophan catabolic process
[Evidence IEA]; GO_process: GO:0006569 - tryptophan
catabolic process [Evidence IGI] [PMID 12499363]"
/codon_start=1
/product="indolepyruvate decarboxylase 1"
/protein_id="XP_018733714.1"
/db_xref="GeneID:30036129"
/translation="MAQLHCQLTQLTQLTILTKRQVFGVPGDFNLALLDHIYEVEGIR
WAGNTNELNAGYAADGYSRIKRLAALVTTFGVGELSALAAVAGSYAEHVGLLHVVGVP
SMSAQNKHLLLHHTLGNGDFTVFKSMSGRITEHASVITDITTAAEQIDKAIRTAYIYQ
RPVYLAIPANLSDVKLDADLLRQPINLELEPNPPEAEAEVIEVVAEMVLKADHPILLI
DACASRHNVMKEVAEIARVTQFPVFTTPMGKGSFDEHDPRFGGVYVGSLTLPEVAKVV
EHADLILSFGALLSDFNTGSFSYHYDTKNIVEFHSDSTKIRSATYPEVKMQLILDKVA
KRLSALPQKKLCEVPPYPPLIKATPESPLKQEWLWTRLTSWLRKGDIIVSETGTSNFG
IIQTKFPQGAVGISQVLWGSIGYSVGALVGATMAASEVDPSRRVILFVGDGSLQLTVT
EISTLVRWGLKPYIFILNNDGYTIERLIRGEKATYNDVHMWKYSKLLDLFNAKDHDAL
QVSTTGELDDLFNDAKFASPTKIRTIELFLPQMDAPTNLIKQAKITAATNSE"
gene complement(<1092052..>1093860)
/gene="MSS4"
/locus_tag="AWJ20_4041"
/db_xref="GeneID:30036130"
mRNA complement(<1092052..>1093860)
/gene="MSS4"
/locus_tag="AWJ20_4041"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881091.1"
/db_xref="GeneID:30036130"
CDS complement(1092052..1093860)
/gene="MSS4"
/locus_tag="AWJ20_4041"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733715.1"
/db_xref="GeneID:30036130"
/translation="MGASFFTNGDYCSSNGGVLSDVNRGIVVPKYGACAQQENRRAAR
RSAFAYHKVLILECSDDDSGVSVGSDETLNEPTVWKPIDIVSEQPYCEESSHTYSAPV
DNDDVNCSEWSFEAGDEAAFTTRQIESARSEEDTEATWSAPAAYWALSDCPIEVDESD
SPIESHGDATLVELPLETTSSDLAESEEITPAKVAETPCAIADIPTSNSSIDSLIAEI
ISVATLYKDLPQLIVSGGHKADSEFELLKTAPLALSKPITHTPSDNLVIQVEEVDKSK
TPLLLTRAKAKLTSFTGRTAPITKSTLMFNGIQTAVYATPLSSHENLTMENKKYRLDG
GAIIKAYSPIVYQNIRTLCGIDCHEFLNSFVLQSDLTKTKSPGKSGSDFLFTPDGKYI
IKTIKRKEHNVIANAEFLADYYNHVKAHPSTQLPFYLGNYTLVAGGKKTHFVIMKNLL
QKETQLIYDLKGSTHDRRAGPRKDNRGRVVFKDLDWTDKHEAISMSQEDRDKLMVQVS
KDVDFLKRHNIMDYSLLVGLQSDTRTCQQQPVIGMIDTLCPFSWRKRAETISKSLIFG
SSAIDVVHPSKYGARFLNFVQSAVVPRPGRSVSIRC"
gene complement(<1096481..>1096882)
/gene="PMU1"
/locus_tag="AWJ20_4042"
/db_xref="GeneID:30036131"
mRNA complement(<1096481..>1096882)
/gene="PMU1"
/locus_tag="AWJ20_4042"
/product="Pmu1p"
/transcript_id="XM_018881092.1"
/db_xref="GeneID:30036131"
CDS complement(1096481..1096882)
/gene="PMU1"
/locus_tag="AWJ20_4042"
/note="Putative phosphomutase; contains a region
homologous to the active site of phosphomutases;
overexpression suppresses the histidine auxotrophy of an
ade3 ade16 ade17 triple mutant and the temperature
sensitivity of a tps2 mutant; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0016853 - isomerase activity [Evidence
IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0008150 - biological_process
[Evidence ND]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]"
/codon_start=1
/product="Pmu1p"
/protein_id="XP_018733716.1"
/db_xref="GeneID:30036131"
/translation="MIYTWDGILLDPPPLVVDDLRETIGLEPCNKRRSRTHISTRFGH
VVNIEDSFPEEDTTWRPDHRETPLEHSIRTKRFLTRLFDSDWHSPTPDDYVSVTSHMG
TINSFLLVINHRPFTVLPGGMIPVIIRADRV"
gene <1098756..>1100837
/gene="CDC5"
/locus_tag="AWJ20_4043"
/db_xref="GeneID:30036132"
mRNA <1098756..>1100837
/gene="CDC5"
/locus_tag="AWJ20_4043"
/product="polo kinase CDC5"
/transcript_id="XM_018881093.1"
/db_xref="GeneID:30036132"
CDS 1098756..1100837
/gene="CDC5"
/locus_tag="AWJ20_4043"
/inference="similar to AA sequence:KEGG_Orthology:K06660"
/note="Polo-like kinase; controls targeting and activation
of Rho1p at cell division site via Rholp guanine
nucleotide exchange factors; regulates Spc72p; also
functions in adaptation to DNA damage during meiosis; has
similarity to Xenopus Plx1 and S. pombe Plo1p; possible
Cdc28p substrate; GO_component: GO:0005935 - cellular bud
neck [Evidence IDA] [PMID 10594031]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9819423];
GO_component: GO:0000922 - spindle pole [Evidence IDA]
[PMID 10594031]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0008047 - enzyme
activator activity [Evidence IDA] [PMID 16763112];
GO_function: GO:0016301 - kinase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0004672 - protein kinase activity
[Evidence IEA]; GO_function: GO:0004672 - protein kinase
activity [Evidence IDA] [PMID 12637549]; GO_function:
GO:0004672 - protein kinase activity [Evidence IDA] [PMID
16319894]; GO_function: GO:0004672 - protein kinase
activity [Evidence IDA,IMP] [PMID 17122856]; GO_function:
GO:0004672 - protein kinase activity [Evidence IDA,IMP]
[PMID 8321244]; GO_function: GO:0004674 - protein
serine/threonine kinase activity [Evidence IEA,IEA];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_function: GO:0016772 - transferase activity,
transferring phosphorus-containing groups [Evidence IEA];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0051301 - cell division [Evidence IEA];
GO_process: GO:0007067 - mitotic nuclear division
[Evidence IEA]; GO_process: GO:0016310 - phosphorylation
[Evidence IEA]; GO_process: GO:0010696 - positive
regulation of spindle pole body separation [Evidence
IGI,IMP] [PMID 18500339]; GO_process: GO:0008104 - protein
localization [Evidence IMP] [PMID 16763112]; GO_process:
GO:0006468 - protein phosphorylation [Evidence IEA];
GO_process: GO:0006468 - protein phosphorylation [Evidence
IDA] [PMID 16319894]; GO_process: GO:0006468 - protein
phosphorylation [Evidence IDA,IMP] [PMID 17122856];
GO_process: GO:0006468 - protein phosphorylation [Evidence
IDA,IMP] [PMID 8321244]; GO_process: GO:0000712 -
resolution of meiotic recombination intermediates
[Evidence IMP] [PMID 12717442]; GO_process: GO:0090306 -
spindle assembly involved in meiosis [Evidence IMP] [PMID
20237423]; GO_process: GO:0070194 - synaptonemal complex
disassembly [Evidence IGI] [PMID 18832066]"
/codon_start=1
/product="polo kinase CDC5"
/protein_id="XP_018733717.1"
/db_xref="GeneID:30036132"
/translation="MIYAAKTVAKASLKSSKTKEKLLAEIKIHKSMHHPNIVQFVDCF
EDDDNVYILLEICENQSLMEMLRARSRLTEEETKYYIVQILGAVRYMHKRRVLHRDLK
LGNIFIGDNMDVKIGDFGLATVLASDSDRRRTICGTPNYIAPEVLFGKKVGHSYEADI
WSVGIILYTMLFGKPPFQTKEIEDIYDRIANLSYSFPEKVKVSEDAKDLIRKLLVTDP
VERLSIEKVLEHPFFEYQFPAMVPSFALTKKPIYAAMTEHDARKNFINCQVQAKIRLA
ESTEVASTDVKPIKIDERAATSAQNSQTENGPAAVLPTSLSPASTKDKYKMVIVPRKS
GLDLKQQQQQKLKLTGEYAGKAVEGNKQTAEQNQDRAYDRHSASPRPRWTRAVTALAT
GNRNQTDKQVAVEQRVTRSATAPPKTGQTNTASRTAVAAAAAAAAAPAASAVSGRTTH
DGKSMLYRTLKTLTIAINSYESKETLAPAQSSPRVPAVFISKWVDFSDKYGLAYQTSE
SNDGVLFRNGTVLLMNPENETYLMLDKDDTTEKPLVCKWTGKAIYPSTENASEHKVIR
LINTFHRYMQDHLQKSNYGIQRASDDLSVNPVFVTYYERQPEYVMLCLNNGTFQFNFP
DHMKLIVSSGASQIDLIDNDQRLHTWSIQEALAFTHRAQHKKLVSDPAYTLIHKLAMC
RTAVKKEYNSL"
gene <1104184..>1104855
/gene="SIN3"
/locus_tag="AWJ20_4044"
/db_xref="GeneID:30036133"
mRNA <1104184..>1104855
/gene="SIN3"
/locus_tag="AWJ20_4044"
/product="transcriptional regulator SIN3"
/transcript_id="XM_018881094.1"
/db_xref="GeneID:30036133"
CDS 1104184..1104855
/gene="SIN3"
/locus_tag="AWJ20_4044"
/codon_start=1
/product="transcriptional regulator SIN3"
/protein_id="XP_018733718.1"
/db_xref="GeneID:30036133"
/translation="MPGHPSPPPPPPPPPPSQSHNMLSIQSLSGAGGPPPPASQSNRS
PPVGPTAALAQQQQQQPSSGPGTSSVGPLPPQQSPQLQTVPPPPSQASGLSSLAQLGG
AAAGTAAQGPPGGHGPPPPSQTASQTGPAPGGLGQPQIPPTSPNQQHQQQQQSAGLPP
SAAQVLQPSPIQSAQQIAEAAAYRPLNVKDALSYLDQVKVQFQNQPDVYNHFLDIMKD
FKSHR"
gene <1105161..>1109414
/gene="SIN3"
/locus_tag="AWJ20_4045"
/db_xref="GeneID:30036134"
mRNA <1105161..>1109414
/gene="SIN3"
/locus_tag="AWJ20_4045"
/product="transcriptional regulator SIN3"
/transcript_id="XM_018881095.1"
/db_xref="GeneID:30036134"
CDS 1105161..1109414
/gene="SIN3"
/locus_tag="AWJ20_4045"
/inference="similar to AA sequence:KEGG_Orthology:K11644"
/codon_start=1
/product="transcriptional regulator SIN3"
/protein_id="XP_018733719.1"
/db_xref="GeneID:30036134"
/translation="MDPNPIRVTTPMGTTSRPDGEPTIPYPGDGRWGGPPQGPGQGLP
PVAGQPQLQGPGQVQVLEQQQVQPPGVPGQQPQGPHPQYQRVPSVTELGPGGAGGLPP
QLQQPQQPPQQPGQQQPQYPGYLAEQQQQQQQQQQQQQQQQQQQQQQQQQQQHPMGPP
DPQTQPFNSNGAMSELHAAANGGRPPMMTEQQRRQGGGPVEFNHAINYVNKIKNRFAS
QPEIYKNFLEILQTYQREQLRIAEVYTQVTLLFKEAPDLLEDFKQFLPDTSQPQGQMG
PGAGGQEAMRLPPVGNFAPPGSIGAGGPAAAIGRDAGIGPGGKKKKGGATNAEQAYQQ
QQQQQQQQLQMQQQQQAISQQYGGYNIDSNNGVPVSSLRSGGPTIAGTGRGGKGKGAG
GNMMIREPSPTLVPAIPEPLPPPSRSGGLHDEIAFFDKVKKFIGNKQTYNEFLKIINL
FTQKIIDKNVLVERVDGFIGNNKELMDWFKRFVKYEGKPLHIENIVHKKHNLDLSLCR
SYGPSYRLLPKSETLMPCSGRDEMCWEVLNDEWVVHPTWASEDSGFVSHRKNQYEEIL
YRVEEERHEYDYYIGSNLRTIQTLETIANRIANLTNEEKAIFKLQPKLGTDSTIYQKI
IRKIYDKEKGLEVIEALHENPAVAVPIVLRRLKQKDEEWKRAHREWNKVWRETEQKIF
YKSLDHIGLTFKQTDKKLLTTRSLVSEITTVKAEQNSKRKNPALPLPKSQLVFKMADN
DVLLDILRLVLSFLEHGGSYSSNDRERMEAFFRSFLELFFSLPPNLISERFPENEGRK
SSSPEAEDESKTDGGAAATAAGAVSNGSTGAGGSKKRAREPSGDLLRDVLKKSRFNGR
ADLSPSAEVELDTETDSPAETPVPSAEDTTGAENTNIPRFGDSEDAWLKHAQTSPSPE
LGAKNDNQKREVFNLFANTTIYVFVRILQILYDRLEEVKGYESLVSEEIANSFNVDFA
NDLGLYDTKLADMGLRFDSKDCYGQLLSLSERLIEGEVEHQWFEEAIRQAYRNRAYKF
YTIDKIVQALVKHVHAIVGDQKSSDVLVLFQADRVEPLSDIKKQIAYRMQVKKIIGSD
ESLFRIDWNDEEKRIGFQFLGNYDLTMRDIKKAEDKWNYYLTSYMMSVPTEGVALDRI
KAPLLYRSMIELVDENYPFVIIEQGLMARVCMNTYKLFFEAGTVDFFTRPGVDNSVLR
TPAVLEGRKNRWSSIIDGPDGWKSGLSETDIKNTEDRFKIWEEQGPEAFEKWEAPVVA
VEPEATANGTSDEQTNQTEAAVVPTGETTVDEAKPLEAPADAMEVETTSNGVKAADDT
TSEPAAGEPSSEKSEVVAPESKLTTDDEPKPKLDASSEVKSENDSKPEPSAGEQEASA
AAATETSSPSSAPTSTTNPAGDADQQPPKAADGDVEMSG"
gene complement(<1112750..>1113700)
/gene="PFA4"
/locus_tag="AWJ20_4046"
/db_xref="GeneID:30036135"
mRNA complement(<1112750..>1113700)
/gene="PFA4"
/locus_tag="AWJ20_4046"
/product="Pfa4p"
/transcript_id="XM_018881096.1"
/db_xref="GeneID:30036135"
CDS complement(1112750..1113700)
/gene="PFA4"
/locus_tag="AWJ20_4046"
/inference="similar to AA sequence:KEGG_Orthology:K18932"
/note="Palmitoyltransferase with autoacylation activity;
required for palmitoylation of amino acid permeases
containing a C-terminal Phe-Trp-Cys site; required for
modification of Chs3p; member of the DHHC family of
putative palmitoyltransferases; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IDA] [PMID
16647879]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0016409 - palmitoyltransferase activity
[Evidence IMP] [PMID 16818716]; GO_function: GO:0019706 -
protein-cysteine S-palmitoyltransferase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_function: GO:0016746 - transferase
activity, transferring acyl groups [Evidence IEA];
GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
GO_process: GO:0018345 - protein palmitoylation [Evidence
IMP] [PMID 16751107]; GO_process: GO:0018345 - protein
palmitoylation [Evidence IMP] [PMID 16818716]"
/codon_start=1
/product="Pfa4p"
/protein_id="XP_018733720.1"
/db_xref="GeneID:30036135"
/translation="MTSPGSPTSDFKPLPGEWTRWCIKCNGYKPERTHHCRQCKKCVL
KMDHHCPWTYNCVGHANMPHFVRFLAWVLISASYAFYHVWARGFFLYSKRHLPLSTYD
VTKTEIAFTIVLIPVVSFVLFSVGLLSIRVAWNMVEGQTQIETWEVERIETLVRRKLV
QNVEFPYDLDPWTNVANAMGGNNPLAWMWPFGGPVGDGMHFEKNEAADDGSVWPPDHR
DQGPPRSGSAGASSSPSGDIGIRTAAPRGHYPRTLGYMRHRGNGEYAEDDGDDGYDSD
SEDEEVERLPEENDFYKRDQWMNFEGESIADFGVDVDTES"
gene <1114610..>1116007
/gene="ARG4"
/locus_tag="AWJ20_4047"
/db_xref="GeneID:30036136"
mRNA <1114610..>1116007
/gene="ARG4"
/locus_tag="AWJ20_4047"
/product="argininosuccinate lyase ARG4"
/transcript_id="XM_018881097.1"
/db_xref="GeneID:30036136"
CDS 1114610..1116007
/gene="ARG4"
/locus_tag="AWJ20_4047"
/inference="similar to AA sequence:KEGG_Orthology:K01755"
/note="Argininosuccinate lyase; catalyzes the final step
in the arginine biosynthesis pathway; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 205532];
GO_function: GO:0004056 - argininosuccinate lyase activity
[Evidence IEA,IEA]; GO_function: GO:0004056 -
argininosuccinate lyase activity [Evidence IDA] [PMID
4578972]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0016829 - lyase activity
[Evidence IEA]; GO_process: GO:0006526 - arginine
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0042450 - arginine biosynthetic process via ornithine
[Evidence IEA]; GO_process: GO:0042450 - arginine
biosynthetic process via ornithine [Evidence IDA] [PMID
4578972]; GO_process: GO:0008652 - cellular amino acid
biosynthetic process [Evidence IEA]"
/codon_start=1
/product="argininosuccinate lyase ARG4"
/protein_id="XP_018733721.1"
/db_xref="GeneID:30036136"
/translation="MSSEQPAELKLWGGRFTGGTDPLMELYNASLPYDKKMYVADLEG
TRVYTSALHKVGLLNDHELSEIHRGLKLVEAEWAEGKFVEKPGDEDIHTANERRLGEL
IGRDISGKVHTGRSRNDQVATDMRLYVREELAKIRAILVGLIKVFVSRAEKEMDILMP
GYTHLQRAQPIRWSHWLSSHATYLANDLERLDQVIKRVNRSPLGAGALAGHPYGVDRE
YIADNLGFESVIGNSLTAVADRDFVVETLFWGSLVLNHLSKFSEDLIIYSTAEFGFIK
LADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLAGFMMSLKATPSTYNKDLQEDKE
PLFDCLTTVSHSLSIATGVISTLSVDAAKMRAALTMDMLATDLADYLVRKGVPFRETH
HISGEAVATSEKLGLSGIDQLTLEQYKQIDARFDADVVNVFNFETSVERRTATGGTAK
SAVLAQLKNIASSLN"
gene <1117079..>1118902
/gene="MSS4"
/locus_tag="AWJ20_4048"
/db_xref="GeneID:30036137"
mRNA <1117079..>1118902
/gene="MSS4"
/locus_tag="AWJ20_4048"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881098.1"
/db_xref="GeneID:30036137"
CDS 1117079..1118902
/gene="MSS4"
/locus_tag="AWJ20_4048"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733722.1"
/db_xref="GeneID:30036137"
/translation="MGAFFFTNGDYCPSNGTHKVPRRPVLSDVHCGVSVPKYGACAQL
ESRRPARRSAFAYHKVLILECSPDDSGVSVGSDETLNEPTIWKPVDIVSEQAYCEESS
NISSAPIDNDDDNSSEWSFETGDEAAFTTRQIETAWSEEDTEATWYAPAASWALSGFP
IEIYENDSTPESHGEVTLVELPLETPRSDLAESEEITPAKVAETPCAIADIPTSNPSI
DSLVAEIIAVATLYKNLPQLIVSGGLQADSEFELPKTAPLALSKPIAQTPSDDLVIEE
VDKSKTPLLLTRAKAKLTSFTGHASPITKSTLMFNGIQTAVYATPLSSHENLTMENKK
YRLDGGAIIKAYSPIIYQSIRTLCGIDCHEFLNSFVLQSDLTKTKSPGKSGSDFLFTP
DGKYIIKTIKRKEHNVIANAEFLADYYNHVKAHPSSQLPFYLGNYTLVAGGKKTHFVI
MKNLLQKETQLIYDLKGSTHDRRAGARKDNRGRVVFKDLDWTDKDEAISMSQEDRDKL
MVQVSKDVDFLKRHNIMDYSLLVGLQSDTRTCQQQPVIGMIDTLCPFSWRKKAETISK
SLIFGSSAIDVVHPSKYGARFLNFVQSAVVPGPGRSVSTRC"
gene <1120783..>1121862
/locus_tag="AWJ20_4049"
/db_xref="GeneID:30036138"
mRNA <1120783..>1121862
/locus_tag="AWJ20_4049"
/product="hypothetical protein"
/transcript_id="XM_018881099.1"
/db_xref="GeneID:30036138"
CDS 1120783..1121862
/locus_tag="AWJ20_4049"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733723.1"
/db_xref="GeneID:30036138"
/translation="MVDTTKVQVLLVGSGGVGTIASLALEQSHQVEVTSVIRSDYDIV
VEKGFTIESIDYGNLTGWRPTRVVKTVQEAVNVANGRPYDYIVVCTKVLPEIFKTEDL
IRPAVQDAKTIIVLIQNGIDIEKSVSEAFPANVVLSGVSMIGSTNYGGKIVQFEHDKL
GVGYYSSETTDSKHSKDQLEAEAKKFVDLYSVACKNCTYYPDLKRARWRKLVYNSTIN
TICALTQVDTGRAYLSQLDHALIYPAMAEIITIAETALGEPLDKGIDKIMLESDDGFY
YKPSMQVDVDKGNPIEVEAILGNPLRTAKELNIKTPVLTVVYNLLRALQFRLMESRGY
ITVPENPSRKVSAPLLEPTTLPIEP"
gene <1124805..>1126031
/gene="IME2"
/locus_tag="AWJ20_4050"
/db_xref="GeneID:30036140"
mRNA <1124805..>1126031
/gene="IME2"
/locus_tag="AWJ20_4050"
/product="protein kinase IME2"
/transcript_id="XM_018881101.1"
/db_xref="GeneID:30036140"
CDS 1124805..1126031
/gene="IME2"
/locus_tag="AWJ20_4050"
/inference="similar to AA sequence:KEGG_Orthology:K12765"
/codon_start=1
/product="protein kinase IME2"
/protein_id="XP_018733724.1"
/db_xref="GeneID:30036140"
/translation="MDCMDGTLQDFILSQDGIPPDPSIYQNILKQVLKGLDHIHSHQF
IHRDIKPENILITGDISGPNLVVKVADFNLSKDLSDGPDDAFTSYIGTRWYRAPEVLI
KCERYSTAIDIWAFAAVAYEMATCCVLFEGRDQIHQLKLQVQLLGAPGPNSVCGEFQP
LTNFLEEMSNDAYNEIIEVCDYLNFEPEYLRLFSGDDFWQQLSDECRGDIGSTVFGRK
EKRADDENDPENQISSFNSFSGLNRFCTINHGGKLVPSYTGLSDFNKLLLPILRWDPS
TRPTAGQLLCHPILADEYDHTAGPLTAVPPLVLERTDTAESDDEDQDDDYNIQAPNMI
NRPPLSLAEPNNAPHPQPSNSKNKTSAPFNPRLAQIAAAPNENSEEPFDDNHPSHILS
PNHLYNYWASPYSFHY"
gene complement(<1126288..>1126947)
/gene="TEM1"
/locus_tag="AWJ20_4051"
/db_xref="GeneID:30036141"
mRNA complement(<1126288..>1126947)
/gene="TEM1"
/locus_tag="AWJ20_4051"
/product="Ras family GTPase TEM1"
/transcript_id="XM_018881102.1"
/db_xref="GeneID:30036141"
CDS complement(1126288..1126947)
/gene="TEM1"
/locus_tag="AWJ20_4051"
/inference="similar to AA sequence:KEGG_Orthology:K06682"
/note="GTP-binding protein of the Ras superfamily;
involved in termination of M-phase; controls actomyosin
and septin dynamics during cytokinesis; GO_component:
GO:0005816 - spindle pole body [Evidence IDA] [PMID
19154724]; GO_function: GO:0005525 - GTP binding [Evidence
IEA,IEA]; GO_function: GO:0003924 - GTPase activity
[Evidence IDA] [PMID 12048186]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0005515
- protein binding [Evidence IPI] [PMID 10688190];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0051301 - cell division [Evidence IEA];
GO_process: GO:0007067 - mitotic nuclear division
[Evidence IEA]; GO_process: GO:0007096 - regulation of
exit from mitosis [Evidence TAS] [PMID 16039591];
GO_process: GO:0007264 - small GTPase mediated signal
transduction [Evidence IEA]"
/codon_start=1
/product="Ras family GTPase TEM1"
/protein_id="XP_018733725.1"
/db_xref="GeneID:30036141"
/translation="MSTSQPSHGSTGASGSEPIDLAVPQKNSVSLKVGMIGDAQIGKT
SLMVRYVEGSFDEDYIQTLGVNFMEKSVNIRNTEIKFSIWDLGGQREFVNMLPLVSND
AVAILFMFDLTRKSTLNSIKEWYRQARGFNKTAIPFLVGTKFDAFAEMPDADQEEISR
QAKKFAKAMKASLVFCSTKEMINVQKIFKIVLSKAFQLKLNMNEYTETGEPLLIYQQW
S"
gene <1128006..>1128641
/locus_tag="AWJ20_4052"
/db_xref="GeneID:30036142"
mRNA <1128006..>1128641
/locus_tag="AWJ20_4052"
/product="hypothetical protein"
/transcript_id="XM_018881103.1"
/db_xref="GeneID:30036142"
CDS 1128006..1128641
/locus_tag="AWJ20_4052"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733726.1"
/db_xref="GeneID:30036142"
/translation="MIPLTETGETDADDSVTVMSKRLFHSLRPLGKNPYYFYDLAKKQ
PRQSFDPGHYSFGNLASTEERARQVFGDLGSRDASRMDALHKARTIAGVRVPEKPEEP
TNCCMSGCVNCVWEMYKDDLEDWRAKRKEAKHRLLTDPKYASTKWPSDFGPEPPSRSI
ETNISEEAGELPLDDDEAWDGVDVNIKVFVETEKRLHALRRQRAATSSASL"
gene <1131271..>1132494
/gene="ILV5"
/locus_tag="AWJ20_4053"
/db_xref="GeneID:30036143"
mRNA <1131271..>1132494
/gene="ILV5"
/locus_tag="AWJ20_4053"
/product="ketol-acid reductoisomerase"
/transcript_id="XM_018881104.1"
/db_xref="GeneID:30036143"
CDS 1131271..1132494
/gene="ILV5"
/locus_tag="AWJ20_4053"
/inference="similar to AA sequence:KEGG_Orthology:K00053"
/note="Acetohydroxyacid reductoisomerase and mtDNA binding
protein; involved in branched-chain amino acid
biosynthesis and maintenance of wild-type mitochondrial
DNA; found in mitochondrial nucleoids; GO_component:
GO:0042645 - mitochondrial nucleoid [Evidence IDA] [PMID
10869431]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 18193418]; GO_function: GO:0050662 -
coenzyme binding [Evidence IEA]; GO_function: GO:0003690 -
double-stranded DNA binding [Evidence IDA] [PMID
18023287]; GO_function: GO:0004455 - ketol-acid
reductoisomerase activity [Evidence IEA,IEA]; GO_function:
GO:0004455 - ketol-acid reductoisomerase activity
[Evidence IMP] [PMID 14124940]; GO_function: GO:0004455 -
ketol-acid reductoisomerase activity [Evidence IMP] [PMID
3027658]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_function: GO:0016616 -
oxidoreductase activity, acting on the CH-OH group of
donors, NAD or NADP as acceptor [Evidence IEA];
GO_process: GO:0009082 - branched-chain amino acid
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0009082 - branched-chain amino acid biosynthetic
process [Evidence IMP] [PMID 14124940]; GO_process:
GO:0008652 - cellular amino acid biosynthetic process
[Evidence IEA,IEA]; GO_process: GO:0009097 - isoleucine
biosynthetic process [Evidence IEA]; GO_process:
GO:0000002 - mitochondrial genome maintenance [Evidence
IGI] [PMID 18826960]; GO_process: GO:0000002 -
mitochondrial genome maintenance [Evidence IMP] [PMID
7621838]; GO_process: GO:0055114 - oxidation-reduction
process [Evidence IEA,IEA]; GO_process: GO:0009099 -
valine biosynthetic process [Evidence IEA]"
/codon_start=1
/product="ketol-acid reductoisomerase"
/protein_id="XP_018733727.1"
/db_xref="GeneID:30036143"
/translation="MSSKMLRTAARAARNARIATAPSEKRAFSALAKAAIARPTIAAT
TRVASGVASVEARRGLKQINFGGTEETVYERADWPREKLLDYFKNDTLALIGYGSQGH
GQGLNLRDNGLNVIVGVRKDGASWQQAIDDGWVPGTNLFDVNEAINKGTIVMNLLSDA
AQSETWPQIKPLLTEGKTLYFSHGFSPVFKDLTKVDTPKNIDVILVAPKGSGRTVRTL
FKEGRGINSSYAVWQDVTGKAEEKAQALAVAVGSGYVYKTTFEREVHSDLYGERGCLM
GGIHGMFLAQYEVLRERGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARR
GALDWSPRFKDALKPVFNQLYDSVLDGSETKRSLEFNSQKDYREKFEKELQEIRDLEI
WRVGKEVRKLRPENN"
gene <1135285..>1137090
/gene="MSS4"
/locus_tag="AWJ20_4054"
/db_xref="GeneID:30036144"
mRNA <1135285..>1137090
/gene="MSS4"
/locus_tag="AWJ20_4054"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881105.1"
/db_xref="GeneID:30036144"
CDS 1135285..1137090
/gene="MSS4"
/locus_tag="AWJ20_4054"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018733728.1"
/db_xref="GeneID:30036144"
/translation="MGTFFLTNGDYRPSNGTHKVPRRPVLSDVHCGISVPKYGACAQQ
ENRRPARRSAFAYHKVLILECSDDSGVSVGSDETLNEPTVWKPIDIVAEQTYCEFAPI
YNDDDNSSEWSFETGDEAAFTTRQIETAWSEEDTEATWSAPAASWALSGCPIETDENE
YTPESHGEVTLVESPSETSRSDLAESEEITPAKMAETPCAIADITTSSSSIDSLIAEI
ISVATLYKDLPQLIVSGGLQADSEFELLKTAPLALSKPITHTPSDDLVIQVEEVDKSK
TPLLLTRAKAKLTSFSGRTAPITKSTLMFNGVQTAVYATPLSSHETLTMENKKYRLDG
GAIIKAYSPIVYQSIRSLCGIDCHEFLNSFVLQSDLTKTKSPGKSGSDFLFTPDGKYI
IKTIKRKEHNVIANSEFLADYYNHMKAHPSTQLPFYLGNYTLVAGGKKTHFIIMKNLL
QKETQLIYDLKGSTHDRRAGPRKDNRGRVVFKDLDWTDKHEAISMSREDRDKLLVQVS
EDVEFLKRHNIMDYSLLVGLQSGTRTCQQPVIGMIDTLCPFSWRKKAETISKSLIFGS
SAIDVVHPSKYGARFLNFVQSAVVPGPDRSVSIRC"
gene complement(<1138124..>1140604)
/gene="ERB1"
/locus_tag="AWJ20_4055"
/db_xref="GeneID:30036145"
mRNA complement(<1138124..>1140604)
/gene="ERB1"
/locus_tag="AWJ20_4055"
/product="Erb1p"
/transcript_id="XM_018881106.1"
/db_xref="GeneID:30036145"
CDS complement(1138124..1140604)
/gene="ERB1"
/locus_tag="AWJ20_4055"
/inference="similar to AA sequence:KEGG_Orthology:K14824"
/note="Constituent of 66S pre-ribosomal particles; forms a
complex with Nop7p and Ytm1p that is required for
maturation of the large ribosomal subunit; required for
maturation of the 25S and 5.8S ribosomal RNAs; homologous
to mammalian Bop1; GO_component: GO:0070545 - PeBoW
complex [Evidence IDA,IPI] [PMID 16287855]; GO_component:
GO:0005730 - nucleolus [Evidence IEA,IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 14562095];
GO_component: GO:0005654 - nucleoplasm [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_component: GO:0030687 - preribosome, large
subunit precursor [Evidence IEA]; GO_component: GO:0030687
- preribosome, large subunit precursor [Evidence IDA]
[PMID 11583614]; GO_component: GO:0030687 - preribosome,
large subunit precursor [Evidence IDA] [PMID 17443350];
GO_component: GO:0030687 - preribosome, large subunit
precursor [Evidence IDA] [PMID 23212245]; GO_function:
GO:0003674 - molecular_function [Evidence ND];
GO_function: GO:0043021 - ribonucleoprotein complex
binding [Evidence IEA]; GO_process: GO:0000466 -
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IEA];
GO_process: GO:0000463 - maturation of LSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IEA]; GO_process: GO:0006364 - rRNA
processing [Evidence IEA,IEA]; GO_process: GO:0006364 -
rRNA processing [Evidence IMP] [PMID 11522832];
GO_process: GO:0042273 - ribosomal large subunit
biogenesis [Evidence IEA]; GO_process: GO:0042273 -
ribosomal large subunit biogenesis [Evidence IPI] [PMID
16287855]; GO_process: GO:0042254 - ribosome biogenesis
[Evidence IEA,IEA]"
/codon_start=1
/product="Erb1p"
/protein_id="XP_018733729.1"
/db_xref="GeneID:30036145"
/translation="MAKRKASELKQAESRVDSAKTKDEEEDVLALSGGLVAESDDDSD
AEDRELEKLLGEEADEEDENEDDEEEEENEDEDDEEEDDEDEEEDEEDVDPRILKDLE
NIKFRSLSDDSEDESNVRYEPGSDGKLRPIRPEIDPVYDSDDTDGEETNTIGNIPISA
YDKLPHIGYDIDGKRVMRPASASALDTLLESIDLPEGWTGLLDKNTGGGLKLSAEELD
LIKKIQRGENPDDSVNPYEDTIEYFSSQTEVMPLTAIPEPKRRFIPSKHEAKRVMKIV
RAIREGRIVLKSAEEKAKELEEATHYDIWADNADEIPDHIMTLRAPKLPPPTHDESYN
PPEEYLPDEEEIEQWKNMDPADRERNYVPKKYGSLRRVPGYSEGVRERFERSLDLYLA
PRVRRQKLNIDPDSLIPDLPSPKDLRPFPIKCATIFRGHVGKVRSVSFDPSGQFVATG
GDDGTVRVWELLTGRELWRLDVLKHDNYDNQDESDDEDDEDQMKERASGEDHVEAVEW
NPQTGTGLLAISAGENIYLVVPPVFDVEIENRGREIIEAGWGYASNGASGPSQVKSKD
LREDGDDDEDEVEAAAAEAKKKIFAKWTKPVAKLSDNGVGVVVRCKRTVKKLSWHRRG
DYFVTVSPEGANSAVLIHQLSKHSSQSPFRKSKGIVQDAMFHPFRPHLYVGTQRYVRI
YDLAAQVLTKKLLPGVRWLSSFDIHPRGDNVITSSYDKRMTWHDLDLSSKPYKTLRYH
EKAVRDVSFHKGGLPLFCSASDDGNINVFHGSVYDDMMKNPLLVPLKVLKGHEIKNSL
GILNVVWHPKEAWLLSAGADGTARLWTA"
gene <1141005..>1141865
/gene="HSH49"
/locus_tag="AWJ20_4056"
/db_xref="GeneID:30036146"
mRNA <1141005..>1141865
/gene="HSH49"
/locus_tag="AWJ20_4056"
/product="Hsh49p"
/transcript_id="XM_018881107.1"
/db_xref="GeneID:30036146"
CDS 1141005..1141865
/gene="HSH49"
/locus_tag="AWJ20_4056"
/inference="similar to AA sequence:KEGG_Orthology:K12831"
/note="U2-snRNP associated splicing factor; similar to the
mammalian splicing factor SAP49; proposed to function as a
U2-snRNP assembly factor along with Hsh155p and binding
partner Cus1p; contains two RNA recognition motifs (RRM);
GO_component: GO:0005686 - U2 snRNP [Evidence IDA] [PMID
15565172]; GO_component: GO:0005686 - U2 snRNP [Evidence
IDA] [PMID 16314500]; GO_component: GO:0071004 - U2-type
prespliceosome [Evidence IDA] [PMID 16618970];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0003723 - RNA binding [Evidence IEA];
GO_function: GO:0003723 - RNA binding [Evidence IDA] [PMID
9436903]; GO_function: GO:0003676 - nucleic acid binding
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006397 - mRNA
processing [Evidence IEA]; GO_process: GO:0000398 - mRNA
splicing, via spliceosome [Evidence IPI] [PMID 16314500];
GO_process: GO:0000398 - mRNA splicing, via spliceosome
[Evidence IMP,IPI] [PMID 9436903]"
/codon_start=1
/product="Hsh49p"
/protein_id="XP_018733730.1"
/db_xref="GeneID:30036146"
/translation="MSNRLARAGERNPALTVYVGNLHEEVDEELLYELGIQAGPVVNV
HIPLDRVNGKNSGFGFLEYRSEKDVDYAAQILNGIMLFGQPLRVNKSQQEKANSIDVG
AQVFVGSLDPLVDEKTLSDTFSVFGRLIRPATIMRDENGRSRGFGFISFADFESSDRA
IESMNDQYLMNRKVQLNYAFKKDGKGERHGDEAERLLAAEAKKHNYSLTLGEGGVSSP
GNSSIPKGPRASHSSRDDGPESDSDGDVLNDLSQSKKPRNRGRKRQPRTKHRDDLSGA
NAIPTGPKRG"
gene complement(<1142101..>1143663)
/gene="STE50"
/locus_tag="AWJ20_4057"
/db_xref="GeneID:30036147"
mRNA complement(<1142101..>1143663)
/gene="STE50"
/locus_tag="AWJ20_4057"
/product="Ste50p"
/transcript_id="XM_018881108.1"
/db_xref="GeneID:30036147"
CDS complement(1142101..1143663)
/gene="STE50"
/locus_tag="AWJ20_4057"
/note="Protein involved in mating response;
invasive/filamentous growth, and osmotolerance, acts as an
adaptor that links G protein-associated Cdc42p-Ste20p
complex to the effector Ste11p to modulate signal
transduction; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 9742096]; GO_function: GO:0032093 -
SAM domain binding [Evidence IDA] [PMID 16337230];
GO_function: GO:0019887 - protein kinase regulator
activity [Evidence IDA,IPI] [PMID 10397774]; GO_process:
GO:0000161 - MAPK cascade involved in osmosensory
signaling pathway [Evidence IMP,IPI] [PMID 9742096];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0007050 - cell cycle arrest [Evidence IEA];
GO_process: GO:0034605 - cellular response to heat
[Evidence IMP] [PMID 12455951]; GO_process: GO:0007232 -
osmosensory signaling pathway via Sho1 osmosensor
[Evidence IMP] [PMID 11370856]; GO_process: GO:0000750 -
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [Evidence IMP,IPI] [PMID
8793874]; GO_process: GO:0019236 - response to pheromone
[Evidence IEA]; GO_process: GO:0007165 - signal
transduction [Evidence IEA]; GO_process: GO:0001402 -
signal transduction involved in filamentous growth
[Evidence IMP,IPI] [PMID 9738877]"
/codon_start=1
/product="Ste50p"
/protein_id="XP_018733731.1"
/db_xref="GeneID:30036147"
/translation="MAAFSSQRHSIGNSPLSSDSVMTWDCDTVGKWVSNLGLSSYAQS
FIDNKISGDVLIYLDHEDLIDVGVKSVGHRLTILKSIYQLIVDGNIEVDHSHYVPPTV
VERNSLGLSLSDSVLPGSGAATGSSNGIGSSNGASSSTNGAGNTGSHGPVDMSSSTNS
IYSNNRAGVGSMSDVWAEKIITSFEVRDERISYAEAEIKRLMDGYVRLREDLLPIFRI
VKESKPLPTPDAPLPSPGNTLTAGPSASQSQTPSSHGNTHAQPSPTIVPYANSPTPTS
PPAFMTTFPPGSSNPVNSINNNINTSERGPLSASSISGHPSSASLQLQSNNNSASSSN
LGVTASNQKKTGSSSKGMSPSMAPVNIKSPTGNLSEYYDDAATIRPTARVTRKASLNG
IAPSFNNSSASTPISSAGGSSSSLNTPNSSSSTEPFKSFRVSMDDPCYKVLPAALRKY
KINGDPRQYALLVCYGDQERLLGLEEKPLIIFKELQDAGKRPVFMLRHIEGSHSGAQA
GVVVSGTPGGVL"
gene complement(<1148799..>1151465)
/locus_tag="AWJ20_4058"
/db_xref="GeneID:30036148"
mRNA complement(<1148799..>1151465)
/locus_tag="AWJ20_4058"
/product="hypothetical protein"
/transcript_id="XM_018881109.1"
/db_xref="GeneID:30036148"
CDS complement(1148799..1151465)
/locus_tag="AWJ20_4058"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733732.1"
/db_xref="GeneID:30036148"
/translation="MSGELGSWADSDPESPPPVPRGSSGGGASVGASSSQENGGDGGG
DGDTSQVSSESLDLNNSGSTGTSELWTDRSNGFDAQSNRLRRKTGFSQLPMASEGDLG
PADTGSHVRSDVDGSSDPQSDNRAGSGAGGDIGTRRYNNGSGKGVDSAAESGLLYVTG
GSSNHQPVANDSTGDKFVSDAKGSGDASKGNVAGSNREVGYGSMGDSLSGSGHVNRAN
RVFGSNTGSGVNRVNGANSSNGVNSNDASNGGNRETGSKGVNGVNGVNGRNSGNGANG
VDDSNSSDGANGTVRLSKSQSPRVLGPKGFVKDVETSLEQEIINESSPLLHRLEDSLL
GDSGNTGYTGGVSEDPDEPDSSSPYDDPLNSLSIRSLRVLLYVLTTLWSGWITLLIIN
VFSHISWLDEPSVSSAFTQLDMALMSLAQVISSLMFFRNTNSADRSIEYVTTAIVLLD
LVVVLAVPSQRIKNGSKGGLITTVGSLVTFVLTPLTDRVVEYAGKRRDEGRIHQSVRG
LPINMSSFSQYARMTALFFIRGLILVSVALISTNMVLETYDSAVLQPPGKIIPISLQN
ITYSPIPSPTPSPPYSASFFSPFSIYDESTPNANNNDTTTINNSTIGIHIYCTDINKD
TTNPNLTVLLETGDSPALSFSSWALELHERGDIAQVCYWDRPGFGFSDQAPSPLSAGV
VANILFETLNRTLDEELPSKYVLVSHDVGGLYSRIFASRHCSSIHSMVLVDNLHEDLF
FRRNSPWQGFKYWIQGVFASLGYRNAFGIIFKREGPFHRVYGSAYQRQPKYFFAKLQE
QLSARTRTKSEIIDSNASLASDIPILVITSSASIRTDSEWSDKQRQLTKLTSNTLAWE
ILDGPHDLWRNDATKHELQKLLLDDIKYSRRS"
gene <1152565..>1153131
/locus_tag="AWJ20_4059"
/db_xref="GeneID:30036149"
mRNA <1152565..>1153131
/locus_tag="AWJ20_4059"
/product="hypothetical protein"
/transcript_id="XM_018881110.1"
/db_xref="GeneID:30036149"
CDS 1152565..1153131
/locus_tag="AWJ20_4059"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733733.1"
/db_xref="GeneID:30036149"
/translation="MGEPDSLPENLHSVGVKPIIDGQIFKVEGATLVSHYTPGHTDDH
MVFWLEEEEALFSADNVLGGSTTVFSDLKVYLETLNKMAKIGNGKLGKIYPGHGPVIY
DGPQVIKDYISHRKAREDQILELLNGSSEPLSLSDIAAELYKDISAEASAYIERGVLL
HLDKLLQENRAFKDPDSGEWTSLSRAKL"
gene complement(<1155794..>1157035)
/gene="PSA1"
/locus_tag="AWJ20_4060"
/db_xref="GeneID:30036151"
mRNA complement(<1155794..>1157035)
/gene="PSA1"
/locus_tag="AWJ20_4060"
/product="mannose-1-phosphate guanylyltransferase"
/transcript_id="XM_018881112.1"
/db_xref="GeneID:30036151"
CDS complement(1155794..1157035)
/gene="PSA1"
/locus_tag="AWJ20_4060"
/inference="similar to AA sequence:KEGG_Orthology:K00966"
/note="GDP-mannose pyrophosphorylase (mannose-1-phosphate
guanyltransferase); synthesizes GDP-mannose from GTP and
mannose-1-phosphate in cell wall biosynthesis; required
for normal cell wall structure; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11914276]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_function: GO:0005525 - GTP binding [Evidence IEA];
GO_function: GO:0004475 - mannose-1-phosphate
guanylyltransferase activity [Evidence IEA]; GO_function:
GO:0004475 - mannose-1-phosphate guanylyltransferase
activity [Evidence IDA,IMP] [PMID 9195935]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0016779 - nucleotidyltransferase activity
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA,IEA]; GO_process: GO:0009298 -
GDP-mannose biosynthetic process [Evidence IEA];
GO_process: GO:0009298 - GDP-mannose biosynthetic process
[Evidence IDA,IMP] [PMID 9195935]; GO_process: GO:0009058
- biosynthetic process [Evidence IEA]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0000032 - cell wall mannoprotein biosynthetic process
[Evidence IMP] [PMID 11055399]; GO_process: GO:0006486 -
protein glycosylation [Evidence IMP] [PMID 9195935]"
/codon_start=1
/product="mannose-1-phosphate guanylyltransferase"
/protein_id="XP_018733734.1"
/db_xref="GeneID:30036151"
/translation="MSTKALILVGGGTRGTRFRPISLDTPKVLFPVAGKPMLSHAIDA
VATLPSVKEIILFGFYEEYVFRDFIHDTVRQYPHLSIKYLREYKAMGTAGGLYHFRDE
ILKGSPQHFYVIHADVCSSFPLKELATFFEEKKAKAVILGTRVPAKIANNFGAIVADE
DQKVVHYVEKPESHISTLINAGVYLFDKSFFQVMKEARQKKVQDANDHVYFSEDQNDQ
DILRLEQDVLVKFAEREELYVYETKDFWRQVKEAGSAVAVNSLYLQQLFQSNPKAPGL
AAPSANIVPPVFIDPLAEVDVSAKLGPDVSIGPHAKIGKGARIKDSIILEHAQVKDNG
VVLHSILCSGTKVGQWSRVEGSPNNHNTYTATTEKDGVRMQTVSILATNVSVADEVHV
QNTIVLPHKEIKNDVKNEVIM"
gene complement(<1160690..>1162867)
/gene="YME1"
/locus_tag="AWJ20_4061"
/db_xref="GeneID:30036152"
mRNA complement(<1160690..>1162867)
/gene="YME1"
/locus_tag="AWJ20_4061"
/product="i-AAA protease YME1"
/transcript_id="XM_018881113.1"
/db_xref="GeneID:30036152"
CDS complement(1160690..1162867)
/gene="YME1"
/locus_tag="AWJ20_4061"
/inference="similar to AA sequence:KEGG_Orthology:K08955"
/note="Catalytic subunit of the i-AAA protease complex;
complex is located in the mitochondrial inner membrane;
responsible for degradation of unfolded or misfolded
mitochondrial gene products; serves as a nonconventional
translocation motor to pull PNPase into the intermembrane
space; also has a role in intermembrane space protein
folding; mutation causes an elevated rate of mitochondrial
turnover; GO_component: GO:0031942 - i-AAA complex
[Evidence IDA] [PMID 16267274]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0005743 - mitochondrial inner membrane
[Evidence IDA] [PMID 8688560]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 11914276];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0004176 -
ATP-dependent peptidase activity [Evidence IMP] [PMID
15772085]; GO_function: GO:0004176 - ATP-dependent
peptidase activity [Evidence IGI] [PMID 8688560];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0004222 -
metalloendopeptidase activity [Evidence IEA]; GO_function:
GO:0008237 - metallopeptidase activity [Evidence IEA];
GO_function: GO:0017111 - nucleoside-triphosphatase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_function:
GO:0008233 - peptidase activity [Evidence IEA];
GO_process: GO:0006515 - misfolded or incompletely
synthesized protein catabolic process [Evidence IMP] [PMID
18843051]; GO_process: GO:0006457 - protein folding
[Evidence IMP] [PMID 22993211]; GO_process: GO:0045041 -
protein import into mitochondrial intermembrane space
[Evidence IDA,IMP] [PMID 16966379]; GO_process: GO:0051604
- protein maturation [Evidence IMP] [PMID 16966379];
GO_process: GO:0006508 - proteolysis [Evidence IEA,IEA]"
/codon_start=1
/product="i-AAA protease YME1"
/protein_id="XP_018733735.1"
/db_xref="GeneID:30036152"
/translation="MLSRSRFALGPLRMVASTGRRNAVMSRSQIRSFWTPFSQTSSSS
SLSASPSTPATSATHLLSIKEQSANRSLTNPMVQADFYRNLLASNYPHLVVSRYETPG
VGRNSECDALYVQALKVIGHTEKAEAIERSLMAQAQAQAQAQAQAAAAVSASAGFPSG
PVVGSTTTPDGFNSVLNSPVGRGTRTDPVHIIINETALSSVLKWLKWLIPMGLAGWGL
IVGLTFFTESASILKGAGSDIGSSKLDGANGSQSNVKFSDVHGVDEARAELEEIVDFL
KDPSKFTGLGGKLPKGILLTGPPGTGKTLLARAVAGEAGVPFFFMSGSEFDELYVGVG
AKRVRELFAAARAKAPAIVFIDELDAIGSKRNPKDHAYSKQTLNQLLVDLDGFSQTTG
VIFIAATNFPEMLDKALTRPGRFDKIVHVELPDVRGRVAILKHHMKKVETAVDVDPTT
IARGTSGFSGADIMNLVNQAAIFASQQKAVSVDMSHFEWAKDKILLGAARKTMVLTEE
SRRNTAFHEAGHAIMALYTPGATSLYKATILPRGSALGITFQLPEMDKYDQSKKELLA
RLDVCMGGKIAEEMVNGPENVTSGCSSDLKNATQVARAMVTSFGMCDTIGPVELAENW
SDWSAETRHLAEQEIRNLLVTSENRTREMLKEKYVELERLANGLLEYETLDKDEIEKV
VQGLKIEREKTVTNTVVRGNDIKDERSKGGTKVGLAGPGVKLG"
gene <1161813..>1162091
/locus_tag="AWJ20_4062"
/db_xref="GeneID:30036153"
mRNA <1161813..>1162091
/locus_tag="AWJ20_4062"
/product="hypothetical protein"
/transcript_id="XM_018881114.1"
/db_xref="GeneID:30036153"
CDS 1161813..1162091
/locus_tag="AWJ20_4062"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018733736.1"
/db_xref="GeneID:30036153"
/translation="MAGALARAAANNSRTRLAPTPTYNSSNSEPLIKKKGTPASPATA
LARRVLPVPGGPVRRIPLGSLPPKPVNLEGSFRKSTISSSSARASSTP"
gene complement(<1170305..>1171174)
/locus_tag="AWJ20_4063"
/db_xref="GeneID:30036154"
mRNA complement(<1170305..>1171174)
/locus_tag="AWJ20_4063"
/product="S-formylglutathione hydrolase"
/transcript_id="XM_018881115.1"
/db_xref="GeneID:30036154"
CDS complement(1170305..1171174)
/locus_tag="AWJ20_4063"
/inference="similar to AA sequence:KEGG_Orthology:K01070"
/note="Esterase that can function as an
S-formylglutathione hydrolase; non-essential intracellular
esterase; may be involved in the detoxification of
formaldehyde, which can be metabolized to
S-formylglutathione; similar to human esterase D;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
10427036]; GO_function: GO:0018738 - S-formylglutathione
hydrolase activity [Evidence IEA,IEA]; GO_function:
GO:0018738 - S-formylglutathione hydrolase activity
[Evidence IDA] [PMID 10427036]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_process: GO:0046294
- formaldehyde catabolic process [Evidence IEA];
GO_process: GO:0046294 - formaldehyde catabolic process
[Evidence IMP] [PMID 10427036]"
/codon_start=1
/product="S-formylglutathione hydrolase"
/protein_id="XP_018733737.1"
/db_xref="GeneID:30036154"
/translation="MAGFKTNSTIASCGGKLLKLNHESKVLKSAMDVNVFLPPAAVDG
KKKVPVLFFLSGLTCTPQNCSEKGFFQSAAAQHGIAIVYPDTSPRGSDHPGENDSWDF
GSGAGFYINATEKPWSDNYNMYDYVVSELPEALFEEFPQLDADRVSITGHSMGGHGAL
TLFLRNPGKYKSVSAFSPISNPINAPWGIKAFSGYFGEQNKAKWAEHDATELIKKYSG
PSPEILIDVGTGDQFYKDKQLLPENLVEAANDSANYKGKVNLRLQPDYDHSYYFISSF
AKDHVEHAAKYLL"
gene complement(<1171610..>1173238)
/gene="DMA2"
/locus_tag="AWJ20_4064"
/db_xref="GeneID:30036155"
mRNA complement(<1171610..>1173238)
/gene="DMA2"
/locus_tag="AWJ20_4064"
/product="ubiquitin-conjugating protein DMA2"
/transcript_id="XM_018881116.1"
/db_xref="GeneID:30036155"
CDS complement(1171610..1173238)
/gene="DMA2"
/locus_tag="AWJ20_4064"
/note="Ubiquitin-protein ligase (E3); controls septin
dynamics and spindle position checkpoint (SPOC) with
ligase Dma1p by regulating recruitment of Elm1p to bud
neck; regulates levels of eIF2 subunit Gcd11p, as well as
abundance, localization, and ubiquitination of Cdk
inhibitory kinase Swe1p; ortholog of human RNF8, similar
to human Chfr; contains FHA and RING finger domains; DMA2
has a paralog, DMA1, that arose from the whole genome
duplication; GO_component: GO:0032177 - cellular bud neck
split septin rings [Evidence IMP] [PMID 23442799];
GO_component: GO:0005934 - cellular bud tip [Evidence IMP]
[PMID 23442799]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_function: GO:0016874 -
ligase activity [Evidence IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0004842
- ubiquitin-protein transferase activity [Evidence
IDA,IMP] [PMID 18202552]; GO_function: GO:0008270 - zinc
ion binding [Evidence IEA]; GO_process: GO:0007049 - cell
cycle [Evidence IEA]; GO_process: GO:0051301 - cell
division [Evidence IEA]; GO_process: GO:0032186 - cellular
bud neck septin ring organization [Evidence IGI] [PMID
22570619]; GO_process: GO:0000132 - establishment of
mitotic spindle orientation [Evidence IGI] [PMID
15146058]; GO_process: GO:0000132 - establishment of
mitotic spindle orientation [Evidence IGI] [PMID
22570619]; GO_process: GO:0007067 - mitotic nuclear
division [Evidence IEA]; GO_process: GO:0031578 - mitotic
spindle orientation checkpoint [Evidence IGI] [PMID
15146058]; GO_process: GO:0031578 - mitotic spindle
orientation checkpoint [Evidence IGI] [PMID 22570619];
GO_process: GO:0051865 - protein autoubiquitination
[Evidence IDA] [PMID 18202552]; GO_process: GO:0097271 -
protein localization to bud neck [Evidence IGI] [PMID
22570619]; GO_process: GO:0042787 - protein ubiquitination
involved in ubiquitin-dependent protein catabolic process
[Evidence IGI] [PMID 21562220]; GO_process: GO:0000921 -
septin ring assembly [Evidence IGI] [PMID 15146058];
GO_process: GO:0000921 - septin ring assembly [Evidence
IGI] [PMID 22570619]"
/codon_start=1
/product="ubiquitin-conjugating protein DMA2"
/protein_id="XP_018733738.1"
/db_xref="GeneID:30036155"
/translation="MIRQIRPLDIQSARLTSSDTISVPLPEGSSPMASPSSNASTPTS
PVNDLSSTLNANSINNNNGGHYATLVNAPAGLNHGEPGPVVSHSSNITNGNSGDVTLN
LFESSSGSTPSSPHFIASDSTTPVPGSTVVAGLGGLGGPRGSISNNSNNAGIGTTRAD
GSRDVIVTPTTYNASDASQTQHQHHQHTHSRPHSHSHSRSHSRSLSYTHQTQLEQQEQ
QQNGSQEIEDGFPNQIEIPSPEQMQVLSQVHASDSSTPSPDPAKYPYSIRLSPFVDHS
SSMPAFYTGAVEREGRDGTVIKIGRFDNKRDQRTQGDENPHVSPIVFKSKVVSRSHAE
FLVKDGGQWFVRDVRSSSGTFLNHIRLS 