LOCUS NC_031673 2277569 bp DNA linear CON 25-OCT-2016
DEFINITION Sugiyamaella lignohabitans strain CBS 10342 chromosome D, complete
sequence.
ACCESSION NC_031673
VERSION NC_031673.1
DBLINK BioProject: PRJNA342695
BioSample: SAMN04417247
Assembly: GCF_001640025.1
KEYWORDS RefSeq.
SOURCE Sugiyamaella lignohabitans
ORGANISM Sugiyamaella lignohabitans
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Trichomonascaceae;
Sugiyamaella.
REFERENCE 1 (bases 1 to 2277569)
AUTHORS Bellasio,M., Peymann,A., Valli,M., Sipitzky,M., Graf,A., Sauer,M.,
Marx,H. and Mattanovich,D.
TITLE Complete genome sequence and transcriptome regulation of the
pentose utilising yeast Sugiyamaella lignohabitans
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2277569)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (25-OCT-2016) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 2277569)
AUTHORS Peymann,A. and Graf,A.
TITLE Direct Submission
JOURNAL Submitted (18-FEB-2016) Department of Biotechnology, University of
Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna
1190, Austria
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to CP014502.
##Genome-Annotation-Data-START##
Annotation Provider :: INSDC submitter
Annotation Status :: Full annotation
Annotation Pipeline :: Eukaryotic Annotation Propagation Pipeline
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..2277569
/organism="Sugiyamaella lignohabitans"
/mol_type="genomic DNA"
/strain="CBS 10342"
/type_material="culture from holotype of Sugiyamaella
lignohabitans"
/db_xref="taxon:796027"
/chromosome="D"
gene <2427..>2521
/locus_tag="AWJ20_4604"
/db_xref="GeneID:30036755"
tRNA <2427..>2521
/locus_tag="AWJ20_4604"
/product="tRNA-Lys"
/db_xref="GeneID:30036755"
gene complement(<9063..>9809)
/locus_tag="AWJ20_4605"
/db_xref="GeneID:30036756"
mRNA complement(<9063..>9809)
/locus_tag="AWJ20_4605"
/product="hypothetical protein"
/transcript_id="XM_018881687.1"
/db_xref="GeneID:30036756"
CDS complement(9063..9809)
/locus_tag="AWJ20_4605"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736140.1"
/db_xref="GeneID:30036756"
/translation="MSFYQAKAILDRFGLSHGDTKNVKSKDQASDASKEQKGKKRHPQ
KQSGQSQPENQEDSYQGQEDSVPGVTANKFERTAQPGLEAQGQRLPSTFIPTRPPYTP
YEPKWYDRILDLLVGEDEQSPNSRYALICQNCRMHNGLCQFGEKPQFVVYYCPHCGMQ
NGEEEHPEDDPKESKEAGRQNVKADEGRSEQESTKKVDAEIDIKNGYREYREGNGSED
EANYPNEPVNIKRKTSQKSVRQRKSRSQKE"
gene complement(<10902..>14051)
/gene="POL92"
/locus_tag="AWJ20_4606"
/db_xref="GeneID:30036757"
mRNA complement(<10902..>14051)
/gene="POL92"
/locus_tag="AWJ20_4606"
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 3"
/transcript_id="XM_018881688.1"
/db_xref="GeneID:30036757"
CDS complement(10902..14051)
/gene="POL92"
/locus_tag="AWJ20_4606"
/note="allele of CaO19.8194"
/codon_start=1
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 3"
/protein_id="XP_018736141.1"
/db_xref="GeneID:30036757"
/translation="MSELDIILVQEPGLNKGGTVIQHSSWVAVHSGEGKQRTNAITYI
SAKLRGRLPVIMDRGLTNENMVSITLGNLTITNIYNRQSEELHPLKQWCHRVEGDDSG
NLRVIAGDFNLHHPLWQTNALASRDSKMWVDWASDHDLMLASRADEATHMAGNTIDLV
FTDSRINTTMRESLDVGSDHFMLKWNLHTELVQAELVCTPTQGFNYKKADWEVFAEEV
NRLSKQMDVPSSLTTSKTTENCLKSADKFAERIVAILQEALKISTPKLNITFRSKRWW
TPELKQLKLRVQRLRRRAKRYPTEAHIKERQEANARYKRELMIAKAKNWNGFLSQVTT
QNVWLALKLVKPTENQVSLLPALRIDDDKHTQTYGEQISELFNSLFPSVPPSTKGVKP
VEASANWPKLRKKEVQSALQDQASYKAPGPDGVKTKAIKEAWKQKSFRKLVKGLFSYC
LRVGYHPKIFRKGLTVVLPKPKRDPTLARSYRPITLLSTLGKVLEKIFQRRLTALTAT
CIPRQQFGGRHGFSAVDALTKLSEDIQYNLSRSKVTSILAIDIKGAFDNVHRDSLLST
MEQMNLPVPVQNWVYHFMCGRRTSLMVDGKVTRERKINTGIPQGSPISPLLFLIYSSP
LYSIVQENGGKVIGFVDDITIYVTGKSRDENTKRLSNILQKCHEWATSAHTAIDYGEK
LGFMHVDNQRTSKKRQLRLVLPSGERRQASPTLKLLGVTLDSQWKFSKHVDAIAIKGK
MVLGIIRRLGGITWGVTGASMRNLYQGCVRPILEYASPVWYPKITKQEREQLQRIQNT
GLRAILGGYHQTPIDCLHRDTDIMPLEQRYDTLQDNYIVRLHRNVDPENPVNAESTWW
RKHMEHNELVQRLYEVLPKEEIFQDRIRRRKPPWKKEDMECESKAWKVKSELKKKIYQ
RHHTIWETQYRTSAKGEFYRSYTLPRLYNADHKNPLRYFLNECSKNELSKLVQLRTAK
GAFGMFFKRFKINNRPHQCECGEEEDVKHLLCECPVTENHRQILRDASATLDLKVLLD
SKKGLKAVLAFLAKAPQLL"
gene <16060..>16131
/locus_tag="AWJ20_4607"
/db_xref="GeneID:30036758"
tRNA <16060..>16131
/locus_tag="AWJ20_4607"
/product="tRNA-Gln"
/db_xref="GeneID:30036758"
gene <16659..>18428
/gene="STR2"
/locus_tag="AWJ20_4608"
/db_xref="GeneID:30036759"
mRNA <16659..>18428
/gene="STR2"
/locus_tag="AWJ20_4608"
/product="Str2p"
/transcript_id="XM_018881689.1"
/db_xref="GeneID:30036759"
CDS 16659..18428
/gene="STR2"
/locus_tag="AWJ20_4608"
/inference="similar to AA sequence:KEGG_Orthology:K01739"
/note="Cystathionine gamma-synthase, converts cysteine
into cystathionine; STR2 has a paralog, YML082W, that
arose from the whole genome duplication; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0003962 - cystathionine
gamma-synthase activity [Evidence IEA]; GO_function:
GO:0003962 - cystathionine gamma-synthase activity
[Evidence IMP] [PMID 10821189]; GO_function: GO:0030170 -
pyridoxal phosphate binding [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0008652 - cellular amino acid biosynthetic
process [Evidence IEA]; GO_process: GO:0009086 -
methionine biosynthetic process [Evidence IEA];
GO_process: GO:0006790 - sulfur compound metabolic process
[Evidence IMP] [PMID 10821189]; GO_process: GO:0019346 -
transsulfuration [Evidence IMP] [PMID 10821189]"
/codon_start=1
/product="Str2p"
/protein_id="XP_018736142.1"
/db_xref="GeneID:30036759"
/translation="MPSIDSPPTAVGCPIPSNTPHAVSVTLPSWAANVAYEEGENWVA
SKMSSGYPRFFVHHRIQALSDEIESSYGREGEKSLIFPSYATACRCRDFIKRYSTLQP
CPVRIVQLSTPKPSEGQDDGSSIQAHIAVVFFPQSEYSVARKYWQHTGEGVSSRLGEY
FLEHWGDNNPHNGGGNGANTSSGKGSYKDFHRRSSQSPVLEGLSENSNSNADSNKEQS
TFVEERFGRNLDLSFAPAAKLALRRRIAGKITESGTSASRHGNFEELSEDDVYLYATG
MTSIYSAHRAVLEALGDGKKKSVCFGFPYTDTLKILEKWGPGVHFYGNGEQPDLAALE
KLLEDGEQIVALFCEFPSNPLLKSPDLVNIRRLADKYNFAVVVDETVGNFVNIHVLPY
SDIVVSSLTKVFSGESNVMGGSLVLNPRGKHFKALKDTLGRQYEDNFWAEDAIYLERN
SRDFAKRNEKININTEAVTELLRQSPIIKDLFYPKYVSTKEYYDTCRVDGGGYGGLFS
IVFHNPQHAKNFFDRMVMAKGPSLGTNFTLSCPYTIIAHYLELDFVEKFGVDRHLVRI
SVGLEETEELLERFRKGLEACVE"
gene complement(<20297..>20980)
/locus_tag="AWJ20_4609"
/db_xref="GeneID:30036760"
mRNA complement(<20297..>20980)
/locus_tag="AWJ20_4609"
/product="hypothetical protein"
/transcript_id="XM_018881690.1"
/db_xref="GeneID:30036760"
CDS complement(20297..20980)
/locus_tag="AWJ20_4609"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736143.1"
/db_xref="GeneID:30036760"
/translation="MPEEFVPAWKKLGLKVKQTDPLAVGIVGTEQNENVKRKRKEPKQ
ETDANAEKGPKKPPKRVKLPKNERPPPPEADQLAYLRQYHEDKENWKFSKQKQNWILK
NLYTIEESYAEALDSYVDGLVGGARDRVVEEARKVISEWNEFMTKEDEEEKEKEESTS
KEVAEKEEKIEESNAESSKSETSENSKPKSKTQEPPQPVAPSESKAIKARHFLELLVG
EKPKLEFID"
gene <22086..>23129
/gene="DAL2"
/locus_tag="AWJ20_4610"
/db_xref="GeneID:30036762"
mRNA <22086..>23129
/gene="DAL2"
/locus_tag="AWJ20_4610"
/product="allantoicase"
/transcript_id="XM_018881692.1"
/db_xref="GeneID:30036762"
CDS 22086..23129
/gene="DAL2"
/locus_tag="AWJ20_4610"
/inference="similar to AA sequence:KEGG_Orthology:K01477"
/note="Allantoicase; converts allantoate to urea and
ureidoglycolate in the second step of allantoin
degradation; expression sensitive to nitrogen catabolite
repression and induced by allophanate, an intermediate in
allantoin degradation; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0004037
- allantoicase activity [Evidence IEA,IEA]; GO_function:
GO:0004037 - allantoicase activity [Evidence ISS] [PMID
1916277]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_process: GO:0000256 - allantoin
catabolic process [Evidence IEA,IEA]; GO_process:
GO:0000256 - allantoin catabolic process [Evidence IMP]
[PMID 1916277]; GO_process: GO:0006144 - purine nucleobase
metabolic process [Evidence IEA]"
/codon_start=1
/product="allantoicase"
/protein_id="XP_018736144.1"
/db_xref="GeneID:30036762"
/translation="MSVSQVSLEEFEKRTAAYVNVINTHLETEIIAFSDEFFAEATNL
ITSTPPVREAGRYTEKGAWYDGWETRRHNTEEADWVIIKVGVNSARIAAVEVDTAFFN
GNHAPAISVEGVYLEGKVNQSSFDTVQWQPIISHVECGPSQRHFFARDDGLTKQSFNY
LRLRMYPDGGIARFRAYGSPVPVFPDDKSVVLDLASISNGGSAVAWSDKRFSGPDNLL
LPGRGHDMSDGWETARSRTKGHVDWAIIKLGAPARISKVVVDTAYFLGNFPAAVKVEA
INLDPSSEISATDSAWKDIVGETPAGPGKEHEFSLANGLATITKDELFTHVKLVMIPD
GGVKRLRIFGTRV"
gene complement(<25250..>27241)
/gene="MNS1"
/locus_tag="AWJ20_4611"
/db_xref="GeneID:30036763"
mRNA complement(<25250..>27241)
/gene="MNS1"
/locus_tag="AWJ20_4611"
/product="mannosyl-oligosaccharide 1,2-alpha-mannosidase"
/transcript_id="XM_018881693.1"
/db_xref="GeneID:30036763"
CDS complement(25250..27241)
/gene="MNS1"
/locus_tag="AWJ20_4611"
/inference="similar to AA sequence:KEGG_Orthology:K01230"
/note="Alpha-1,2-mannosidase; involved in ER-associated
protein degradation (ERAD); catalyzes the removal of one
mannose residue from a glycosylated protein, converting
the modification from Man9GlcNAc to Man8GlcNAc; catalyzes
the last step in glycoprotein maturation in the ER and is
critical for ER protein degradation; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IEA];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 8864657]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_function: GO:0005509 - calcium ion
binding [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0016798
- hydrolase activity, acting on glycosyl bonds [Evidence
IEA]; GO_function: GO:0004571 - mannosyl-oligosaccharide
1,2-alpha-mannosidase activity [Evidence IEA,IEA];
GO_function: GO:0004571 - mannosyl-oligosaccharide
1,2-alpha-mannosidase activity [Evidence IDA] [PMID
12090241]; GO_function: GO:0004571 -
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[Evidence IMP] [PMID 1714453]; GO_function: GO:0004571 -
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[Evidence IMP] [PMID 8439291]; GO_function: GO:0004571 -
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[Evidence IDA] [PMID 8910350]; GO_process: GO:0030433 -
ER-associated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 8905927]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0035977 - protein deglycosylation involved in
glycoprotein catabolic process [Evidence IMP] [PMID
9732283]; GO_process: GO:0006486 - protein glycosylation
[Evidence IEA]"
/codon_start=1
/product="mannosyl-oligosaccharide 1,2-alpha-mannosidase"
/protein_id="XP_018736145.1"
/db_xref="GeneID:30036763"
/translation="MTSSSSEDENIFEDCEKTEPGLASTSSSASPNITSAMWRPNTER
RPLPMYKDKPEKTTRRESPSVRKIRKTVGFIVFFTGILYLVYNIGKATAPLPGRGRTY
KDILSWGSKPKEPETWPEKQQAVKNAFIDSWNGYKKYAWGKDVYRPVSKTGVNMGPKP
LGWIIVDSLDTLHIMGLKDQLKEARTWVHKDLDYNMDYEVNTFETTIRMLGGMLSAHY
LTKDDMYLDKAADLGNRLLGAFDSISGIPYSSVNLHSSKGKKSHTDLGAASTAEAATL
QLEFKYLAKLTGESLYWEKVEKILSIMDANKVRSGLVPIYIQPDTGQFSSRVIRLGSR
GDSYYEYLIKQYLQTNEEEPIYKEMYDESVQGIKDHLIKQSTPNKLTFIGELNTGVGG
KLSTKMDHLVCFAGGMLAIGATNGLPLDDARRKGFWSTVREADIQLAMELTHTCYEMY
SQTQTGLAPEIVYFNTDKDSTSDFTIKRNDGHNLQRPETVESLFILWRLTKNPIYREW
GWNIFKSFEHWTKLQGDAGYASINDVRNTPPAFRNNMESFWLSETLKYLYLLFDDSDE
LLPLTDVVFNTEAHPFPKFSMDPLFKTGWSRSKKATAPTPGNAAADEPLQKPIDQTDK
KPKPISKDDRKEASNAEARAEDIPVRQVGAADTAPSISE"
gene complement(<28542..>29183)
/locus_tag="AWJ20_4612"
/db_xref="GeneID:30036764"
mRNA complement(<28542..>29183)
/locus_tag="AWJ20_4612"
/product="peptidase family M20 protein"
/transcript_id="XM_018881694.1"
/db_xref="GeneID:30036764"
CDS complement(28542..29183)
/locus_tag="AWJ20_4612"
/inference="similar to AA sequence:KEGG_Orthology:K01438"
/codon_start=1
/product="peptidase family M20 protein"
/protein_id="XP_018736146.1"
/db_xref="GeneID:30036764"
/translation="MMFNSITYAVALASVFSNVVAEKQSTFSASTLKVNQELLDLHRD
LIQLKSVSGYEIDAAVYLQNYLESNNFTVELETVYGDSNRKNVYAYYGKERNTKALLT
SHIDTVPPYFNYSVVDNRIYGRGSVDAKSCVSSQIVALSQLFKDGEVEEGDVALLFVV
DEEVQGRGMVYASDNLGVDSWEHVIFGEPTENRLGVGHKGMASAQLKAHGKAA"
gene <30080..>30913
/locus_tag="AWJ20_4613"
/db_xref="GeneID:30036765"
mRNA <30080..>30913
/locus_tag="AWJ20_4613"
/product="hypothetical protein"
/transcript_id="XM_018881695.1"
/db_xref="GeneID:30036765"
CDS 30080..30913
/locus_tag="AWJ20_4613"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736147.1"
/db_xref="GeneID:30036765"
/translation="MPLELSAIDVLVTDWDETVTSEDTMAVLSQAAYDHKTNFHPPWE
YFGDMYYKDYEKYACNYNERRGSLQEEAEFLSGLKQVELESVTRVENSGLFKDVPESM
VRSQASKVKIRQGWWDVLSLATKNKVPIVILSVNWSDVLIEEVLSLHGFDTTNSSIRI
YANKIQVDDETGLATGKLGGHPILGSAGIRTAQDKLDILKKIRSQKLSGKATESIAYF
GDSSTDTLALLEADLGVVIHKNSLASRLEGLGVAILRKEHDKTDNNQVYYLCEWSDLL
T"
gene <32007..>33749
/gene="POL4"
/locus_tag="AWJ20_4614"
/db_xref="GeneID:30036766"
mRNA <32007..>33749
/gene="POL4"
/locus_tag="AWJ20_4614"
/product="Pol4p"
/transcript_id="XM_018881696.1"
/db_xref="GeneID:30036766"
CDS 32007..33749
/gene="POL4"
/locus_tag="AWJ20_4614"
/inference="similar to AA sequence:KEGG_Orthology:K10981"
/note="DNA polymerase IV; undergoes pair-wise interactions
with Dnl4p-Lif1p and Rad27p to mediate repair of DNA
double-strand breaks by non-homologous end joining (NHEJ);
homologous to mammalian DNA polymerase beta; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence TAS] [PMID 9745046];
GO_function: GO:0003677 - DNA binding [Evidence IEA];
GO_function: GO:0003887 - DNA-directed DNA polymerase
activity [Evidence IEA,IEA,IEA]; GO_function: GO:0003887 -
DNA-directed DNA polymerase activity [Evidence TAS] [PMID
9745046]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0016779 -
nucleotidyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_process: GO:0006281 - DNA repair [Evidence
IEA,IEA]; GO_process: GO:0006261 - DNA-dependent DNA
replication [Evidence IEA,IEA,IEA]; GO_process: GO:0006974
- cellular response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0006302 - double-strand break repair
[Evidence IDA] [PMID 12235149]; GO_process: GO:0006303 -
double-strand break repair via nonhomologous end joining
[Evidence IDA,IMP] [PMID 10438542]; GO_process: GO:0006303
- double-strand break repair via nonhomologous end joining
[Evidence IDA] [PMID 15342630]"
/codon_start=1
/product="Pol4p"
/protein_id="XP_018736148.1"
/db_xref="GeneID:30036766"
/translation="MVPSRPSSKLYQIKSKKYDEYGARVTSSFDKSVSHVLIDPEWDG
SRTLKYLHISAGDIRPEIDKGTIHFVRDSWPSACIENKQLIPESPDYTVQLYSPAPSP
ETTPEPEVSQKLVQTVISPEKPPKLKKRDISTSSNTSLSPEPDTPKQTAIDDLNPVEA
SGANTSIENKAEKKAQDGSGNDELDSVIQEAELQEHAARFLSDDEIEHALQTQRKKYS
APYSRPKRTSDGRVLKGNWDTFLCMKAGEDNIAKPDGPNALVINILSLMLHHYEAEGD
QWRTLAYRKAIATLKKQTVPVRTAAQAVKLPGIGSRLADKIEEINKTGDLKRLKYTQG
EGKFEVLELLKRIYGVGNKTANKWYSEGIRTLDDVRERKDLTANQQTSLDHYEDFAER
IPRDNVTKHFERVRDAVHEIDPEIEATAMGSYRRLQPDCGDIDIILTKRNASRHDLTV
TLAKLLDVLFEQGFAKYTFGGIEANSMRWLGASAHPDDGNKWRRMDILLVPWEERGAA
FIYYTGNDIFNRSMRLLAQKKGYRLNEKGLFLAPHDRKSAGAVGPLVCGEDEEKIFET
LGVPNRPPHKRIIG"
gene <37366..>38799
/gene="SCS2"
/locus_tag="AWJ20_4615"
/db_xref="GeneID:30036767"
mRNA join(<37366..37399,37772..>38799)
/gene="SCS2"
/locus_tag="AWJ20_4615"
/product="phosphatidylinositol-binding protein SCS2"
/transcript_id="XM_018881697.1"
/db_xref="GeneID:30036767"
CDS join(37366..37399,37772..38799)
/gene="SCS2"
/locus_tag="AWJ20_4615"
/codon_start=1
/product="phosphatidylinositol-binding protein SCS2"
/protein_id="XP_018736149.1"
/db_xref="GeneID:30036767"
/translation="MDISPEVLVFHAPFSEQATKPLVLKNTSDRKLAYKIKTTAPKLY
CVRPNASVIEAGQTIEVSIIRQAKDQPKPTEKSKDKFLVLTAPVSDEILENSDISKLW
ATLEETSKDTIVNKKIKVNYEFGTDSTGSSGSHHGETGAATSGSVGSGSLSSAATGTS
ESGDSSRSSGLPVNSHASALAAATSQSEGAGNTSIIDPSQYGTPLRGGHSDNAGAGSV
LGGGSSGDGAGSSPVNATAAGIVAAAAGGAATGSSLSNGSSGESTLKSRNIPSSADTD
RASQRAQEQISSQSKELKAAAAANSSSSTSGSANEPGFKSSQPGSSTAVRQQPSSGVP
LHLVLALALVAFFIGWKLF"
gene complement(<39107..>40453)
/gene="MAP2"
/locus_tag="AWJ20_4616"
/db_xref="GeneID:30036768"
mRNA complement(<39107..>40453)
/gene="MAP2"
/locus_tag="AWJ20_4616"
/product="Map2p"
/transcript_id="XM_018881698.1"
/db_xref="GeneID:30036768"
CDS complement(39107..40453)
/gene="MAP2"
/locus_tag="AWJ20_4616"
/inference="similar to AA sequence:KEGG_Orthology:K01265"
/note="Methionine aminopeptidase; catalyzes the
cotranslational removal of N-terminal methionine from
nascent polypeptides; function is partially redundant with
that of Map1p; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14562095]; GO_function:
GO:0004177 - aminopeptidase activity [Evidence IEA,IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA,IEA]; GO_function: GO:0070006 -
metalloaminopeptidase activity [Evidence IEA];
GO_function: GO:0070006 - metalloaminopeptidase activity
[Evidence IGI,ISA] [PMID 8618900]; GO_function: GO:0008235
- metalloexopeptidase activity [Evidence IEA];
GO_function: GO:0008233 - peptidase activity [Evidence
IEA]; GO_process: GO:0070084 - protein initiator
methionine removal [Evidence IEA]; GO_process: GO:0035551
- protein initiator methionine removal involved in protein
maturation [Evidence IMP] [PMID 11811952]; GO_process:
GO:0006508 - proteolysis [Evidence IEA,IEA]"
/codon_start=1
/product="Map2p"
/protein_id="XP_018736150.1"
/db_xref="GeneID:30036768"
/translation="MGSTEIANGSEKLADKVEDLKIEESTKNVPKSEQAGDEDDDDEG
DDEVAAEGGEEGAAKKKKKKKKNNKKKKKTAGGSGAVPTSQTEPPTVGLSNLFKDNVY
PEGEIVEYKDDNHYRTTSEEKRYLERASFDAFNDLRKAAEAHRQVRQYAQKTIKPGMT
MIEIADLIENATRALTEGNGDSKKAGPGFPTGLSLNHVAAHYTPNKGDTTVLKYEDVM
KVDIGVHVNGRILDSAFTLTFDDKYDNLLAAVRDATNTGVREAGIDVRLGDIGAAIQE
VMESYEVELDGKTFQVKPIRNLNGHNINPFEIHGGKSVPIVKSHDQTKMEEGETFAIE
TFGSTGKGYVHDEGEVSHYAKRTNIGHVPLRLNSAKNLLAVIDKHFGTLPFCRRYLDH
AGEEKYLLALNNLVKSGIVQDYPPLVDIPGSYTAQYEHTILLRPTVKEVVSRGDDY"
gene complement(<41803..>43146)
/gene="MAL31"
/locus_tag="AWJ20_4617"
/db_xref="GeneID:30036769"
mRNA complement(<41803..>43146)
/gene="MAL31"
/locus_tag="AWJ20_4617"
/product="Mal31p"
/transcript_id="XM_018881699.1"
/db_xref="GeneID:30036769"
CDS complement(41803..43146)
/gene="MAL31"
/locus_tag="AWJ20_4617"
/note="Maltose permease; high-affinity maltose transporter
(alpha-glucoside transporter); encoded in the MAL3 complex
locus; member of the 12 transmembrane domain superfamily
of sugar transporters; functional in genomic reference
strain S288C; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA,IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA,IEA]; GO_function: GO:0005352 -
alpha-glucoside:proton symporter activity [Evidence ISS]
[PMID 18179544]; GO_function: GO:0022891 -
substrate-specific transmembrane transporter activity
[Evidence IEA]; GO_function: GO:0022857 - transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0005215 - transporter activity [Evidence IEA];
GO_process: GO:0000017 - alpha-glucoside transport
[Evidence ISS] [PMID 18179544]; GO_process: GO:0008643 -
carbohydrate transport [Evidence IEA]; GO_process:
GO:0000023 - maltose metabolic process [Evidence IEA];
GO_process: GO:0055085 - transmembrane transport [Evidence
IEA]; GO_process: GO:0006810 - transport [Evidence
IEA,IEA]"
/codon_start=1
/product="Mal31p"
/protein_id="XP_018736151.1"
/db_xref="GeneID:30036769"
/translation="MSPDTEKTHHVVDEQDVGSISSIKAVNVILENTKELLEEERQLG
VIDAMKQYPKAMFWSVFFSVAVIMTGYDAQLVTSFYAMPSFIAKYGQYYNGNKEISAA
WQSGLGMGNPIGQLLGSLVVAWPSEKWGRKRVLIVCNLLIAALIFMQFFANNIQVLCV
GEILAGLLWGCFVVIAPSYASEVSPLALRGILTAFINMAFVIGQFVAQGVAAGLESRT
DKWAYKAPFAIQWIWCLILFVGLPFAPESPWYLVRKGRRDEAKKALQRLAWDKAGDGV
SLEKTLNVIEQTDLLERELHTSSTYMDCFKGSNLRRTEICCMVYLIQVICGNPLMGYC
NYFFIQAGLDNQKAFYMGVGTTAIGFVGTALTWVVLSYAGRRTIFNYGLSVMTLLLFI
IAILDCAPSYSSNLGFSWAQASLLDVWTFLYQLTVGPLTFVIIGEISSTKLVSIL"
gene <44455..>45597
/gene="DES1"
/locus_tag="AWJ20_4618"
/db_xref="GeneID:30036770"
mRNA <44455..>45597
/gene="DES1"
/locus_tag="AWJ20_4618"
/product="sphingolipid delta-4 desaturase"
/transcript_id="XM_018881700.1"
/db_xref="GeneID:30036770"
CDS 44455..45597
/gene="DES1"
/locus_tag="AWJ20_4618"
/inference="similar to AA sequence:KEGG_Orthology:K04712"
/codon_start=1
/product="sphingolipid delta-4 desaturase"
/protein_id="XP_018736152.1"
/db_xref="GeneID:30036770"
/translation="MTMSEVKVQASGTAGHSSDGGLSNKSRVKETSEDVENIKIRREN
EFLFTYQEEPHRSRRMEIIKAHPEVTKLVGHEPLTKWIVVGVVALQFSTAWFMRGASF
SSWYFWAAAYFIGATCNSNIFLAIHELSHNLAFKKPLHNKLFAIFANIPIGIPYSAGF
GPYHQLHHKHMGEPDYDTDLPTSLEAMFLSSVAGKAFFATFQLLFYAIRPMCVIKVNM
TWVHYLNIAVQLAVDALFIKLVCHDSSGSLDLMPFYYLLLSAFLAGSLHPIAGHFIAE
HYVLDAPKEYDLKKTALPETYSYYGWLNVFVYNAGYHNEHHDFPYIPWSRIKTLHTIA
SEFYDPLPSYTSWIYVIYRFVFDENVSLWCRVKRSNIKANKGDDQP"
gene <48199..>49290
/locus_tag="AWJ20_4619"
/db_xref="GeneID:30036771"
mRNA <48199..>49290
/locus_tag="AWJ20_4619"
/product="cyclin Pch1"
/transcript_id="XM_018881701.1"
/db_xref="GeneID:30036771"
CDS 48199..49290
/locus_tag="AWJ20_4619"
/codon_start=1
/product="cyclin Pch1"
/protein_id="XP_018736153.1"
/db_xref="GeneID:30036771"
/translation="MSSSISSDSNATTGSPVSSNGSSTTLVNDSSSANTTSNSTHDTK
DVDALDSSKKPERTTKERVDSGKPNHKPETLVPGQNVWIFSMAQVAKSPSAKDGIPTK
HEFIQRAKGVNFIIVTGTQLRLPPMTIYAAALFLHRFYLRHSLKKFHIYEVGAACLFL
ATKVEESNRRLKDVAIACTRVASKDMNKVVDEQSKDYWKWRDTIIYNEELALEALCFD
LTLKSPYEICTELMIKHEMELVEPVRKAGQHFIHDSCKTVLGLIYSAEVIAAAAFYWA
VRSTGTKIDLKPKNGEENKLLGVKKQHVYDAVNMMADFYSTLQETVLSSERKNKYTRM
TPSNKRKASDSSSSTSKKNKPDEAQTVPV"
gene <50474..>53815
/gene="RAD9"
/locus_tag="AWJ20_4620"
/db_xref="GeneID:30036773"
mRNA <50474..>53815
/gene="RAD9"
/locus_tag="AWJ20_4620"
/product="chromatin-binding protein RAD9"
/transcript_id="XM_018881703.1"
/db_xref="GeneID:30036773"
CDS 50474..53815
/gene="RAD9"
/locus_tag="AWJ20_4620"
/inference="similar to AA sequence:KEGG_Orthology:K06661"
/codon_start=1
/product="chromatin-binding protein RAD9"
/protein_id="XP_018736154.1"
/db_xref="GeneID:30036773"
/translation="MSISGGSWFTDDLEIERDHDDKTQPVGGRGDIDQQTQIVNNQIP
NVLLGPFPSFGIGETKPRDSDSTHSNLSNSSKSSARTQKDEPIDLPSGETDVVSDPDP
VAVQPGPESNVTQKLSSMGPSSDEPVGSPSPIALKEGIDGVSRYRRYSGPVSPIRGEA
ANAIARFNSSPGGFESDHEETMNDRDIGGNFVATGLGASQATQLIDTANYESSPLFKG
HKSSQGVVLNFEELASSPLKVRNASVTSTQNDVNVVNNGVGNGIGNFGAIGEGSKQSN
ASSAEDDSIHIHTAFFAAEDSQLQKRRKAINKLKNQEDTAIQIDDEDVVHLNIDKAKS
TRDSQDKPGTVKADKPDILSLDVDPSPSHISDSIQGSQGASSATQIITGVKYSQITRN
TATANSQFQETQRVRLPNAIPNALLISRDQWMSTVDDSDNNDSQERGFTHPASLNTQG
SGRNSQPVITQVLGTPRQEMRDREREKSFKAASSSSKEEDIDMEEAITSDHEEKNVDK
EDEEEEKGPEEIITSSPEKYDDETPVRRGRGRSKRKIEVETPVVSRFKKPRTGNAELQ
PMLGEMHTDIQGGVSASNDRMAQNDSTPDGDNEAEWESEQQKDTDVQSDAVYTNESDQ
IVTSKRTYAKHIVPSPTTGSTEKSVENVVWGVWGTKTWYAVKLVDTTANGLFEQGGAN
TGTLSVRFGNGDVTDLTTDKIRRFDIQVGDKVKLDNDRRPNFEVVELLQRPPVDGSVD
ETVVTTTTSPDGAWVGYEGVCFVKVQHTKTNKQTTVPIRLLYMSGTQISSYLSRRQLH
KNAFQDGSNTLAASLRQQSKALERLTLTNNTSQSSNQFNQFTAIDSGGAKTQPTDHGV
AQRGILSDCVFTISAIDNTKELEALTKTIIRNGGVVLNGGLQELFSVDYSTLEIKYNH
TRNNFTFGGVLAKFAVRTPKYLEGLALGWPCLSFKFIIDSIAEGEMIQDWEPYLLSAG
VSSALDQAYCSYNVRHFHQNWLEGATLADQFINRRLILGRNSILADRIYLHNPDSKDT
AILFLLVAMAPQDKLELVNSRAEVPSGSVLIDMGRKPSTTSQASTSRPQLTQLRSGTS
TTNHPKTIIYNREWIIQCLINQRIV"
gene <58577..>62152
/gene="TDA9"
/locus_tag="AWJ20_4621"
/db_xref="GeneID:30036774"
mRNA <58577..>62152
/gene="TDA9"
/locus_tag="AWJ20_4621"
/product="Tda9p"
/transcript_id="XM_018881704.1"
/db_xref="GeneID:30036774"
CDS 58577..62152
/gene="TDA9"
/locus_tag="AWJ20_4621"
/note="Transcription factor that regulates acetate
production; green fluorescent protein (GFP)-fusion protein
localizes to the nucleus; null mutant is sensitive to
expression of the top1-T722A allele; not an essential
gene; TDA9 has a paralog, RSF2, that arose from the whole
genome duplication; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA,IEA]; GO_function:
GO:0003676 - nucleic acid binding [Evidence IEA];
GO_function: GO:0043565 - sequence-specific DNA binding
[Evidence IDA] [PMID 16785442]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IDA,IDA] [PMID
19111667]; GO_function: GO:0043565 - sequence-specific DNA
binding [Evidence IDA] [PMID 19158363]; GO_process:
GO:0019413 - acetate biosynthetic process [Evidence IMP]
[PMID 23240040]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Tda9p"
/protein_id="XP_018736155.1"
/db_xref="GeneID:30036774"
/translation="MDMSVDVGVGRSDPATAAAAAASVSATAASTAGASSGSRRPTEP
IPAKSAQVKTDKPRPHVCATCTRSFARLEHLKRHERSHTKEKPFQCPVCERCFARRDL
LLRHKQKLHASFEPGRSNSTGGTSTVGGGIGVGGPRGGTKRKSSSVSSASAAAAAVSA
ATARGLGGINSMVVNSSPSSAPRSASLGFDANSNGHVSTAPYSNLLKSQLGGINGGRP
HDTSASGNGTSTNSSSSGSGSAVSSTSPFSAFNGRPRPASFSAASASSYSKYKDIEVL
NSQMYPSAPPEVGFSTPQLLPASLGNLDIPLEDLFDSDPLFINPQLLGQEDKNDGGSG
QPGQSGQAHGQTTGQSSGQQSGQYDPQQQQQQQQHQQQQQQQQQHTPSMGPLDSATGA
MSQTPQQQQQQQGQMQLSTPTQSHGQDAGSNHQPQQGQGQIHNFLQQHAANQAFNESL
YTADTRSSISSNYKLEEDLSPNSVLAMEHDDFSWMPPSSDNYGRPTPQSILNSSSFSP
PPNSSNNNYHLSGMNVANSESPLSLVGGNGQVNLSPNEGSGHMNSDFDHHMSPGIHED
MRDHKAFSLHIDGMDFWNNNNGINNSQSSFDENTLNLNGGNITGMGMRVNRGQVITPQ
LRLHILATLSTPTPFSSSQSPQLPSTLELQRYINSYTENFGKHMPFLHHSLEFTPDNV
PLALGMAAIGALYTFEHASSSNIFEISRSCIHVYLESRREKRGSGDSDGFNSNKSTPI
WLVQALVLGIIYGLFSGEPLANEIAVAQANAVISLAKSAGLHLPPSKYIQQPSPDLEN
AHIEEKWRYFIAAQERIRTMHVVHMISCLLATSYNVVSSLKNEDMKCGSPCDESLWTS
TSSSEWWETISKKGLEDINPQQYFEGPNFNDYLQQLLQKNTLVGKIPQFTLLSLLYAV
HYEIYQRRHTHDSYILNNRVTPSQHEITWLESQKLNIESILRAWETTWSLSPLASLSP
SSQYGPLMSDAIPLSSLAHVRVYVNLSKVKEAFWKRDFNAMNHELDNLAVPTYYESVT
NGSPSSPHKFDGLLEAASYAADAISLWEKHSIKWTLETTASQTFIHTLVSLFDCGLAV
SEFIHRLEIKPQHEWSEDETMLVNRIGKIFYRVLDVVGHDSLDPNDTWARPLNEDGTL
TRPGKLSVLTLLIVAKMLCKAYIWPFALVMGDALQFRSRHLSAFA"
gene complement(<62322..>66023)
/locus_tag="AWJ20_4622"
/db_xref="GeneID:30036775"
mRNA complement(<62322..>66023)
/locus_tag="AWJ20_4622"
/product="hypothetical protein"
/transcript_id="XM_018881705.1"
/db_xref="GeneID:30036775"
CDS complement(62322..66023)
/locus_tag="AWJ20_4622"
/inference="similar to AA sequence:KEGG_Orthology:K17263"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736156.1"
/db_xref="GeneID:30036775"
/translation="MAAGSTEVSSGTISILPMAIRSVILNVTATPSVAHILAESILPG
LYTGSNSGLLLNNNSEHSLDSMDILIDLIKRFGSSISAAEIVKTENVLLQIISGEQGI
IKKRAVTAMGLLARYLTDKNWEKLTAYLIDGLTESSDLSQLRILIQLAGILARSEPTR
FKNDLRPVIDLIIRKGLDVNDEGITGGRNVHRDEDFDDEEEEDNADDDEEANNPELLD
LREASLASLEIIVGLGYQAVETQLNDILAISAYYIKYNPNIIDDEDDQDEDIDDEDED
IDDNDDDVEMEGHDGGEDSEFEVSDDEDAFSDDDDQSWKLRRYAAKLAAAVAIYIPTQ
LPRIYSQLLPPLVSMYASEKESTVKTEVISCLSEFFKAASDKGLYYSSKLSSNIATRR
SSDISMATDMDPKLFLERHYLRKLVRVSLSELHSSTNTVILQSILTGILGNLVSVLHT
RPSIQTSKIIRSLISLSKTQPGLMIDMLQLVSGIIRAQTTQELTDSLPQLLEIIVLGI
YDKYYKVCGAALDVVLDLVAIHSDQSIKVDISVLLPALIEKAKSTSLDLDTRSKAVKA
LGQFIAKNDLSGSNIDTGCKLILDLLNNEGLRLTSVESISEIASGATVRQVSSKWIIS
SLEGLGNFLNQSSRTLRIGSLNAILSVTNRQPSSDQATVQALANIGQIILNHGKSILT
SSPDAVALDLVSKILTNLVPFISVSEASEFTIQTINQTSSQTAVQISDSILGLLAAVV
QHSTDAAALNAVYEELCRPRHVASASAGSSIGTNQNVELAAKALAVVITEGQMVDKIP
SFETVVAQNGEDLKWSLLVLGYVGKLLPYTTSLEVLYDELNNNARDEITKTVIANSLG
LIISNNVNNYLPDLLNRLKAAGSNTIHLHLLIIREIVSGAKTADVEAYSDDIWNSLFT
SLDAVSSSGIGKVALAASTAAAGIVPNAEASVVSGGDLILAECLGRLSILNPRVFLPQ
LQVQLASEKPSVRVTVLSAVKFTFGQSHDTYDDLLRPIIADFLALVDDDDTGIRQVAL
SALVSAIHNKSHLLLPHLARLLPLLYRETRINTSLIHTVQMGPFKHKVDNGLELRKTA
YETIYTLVSTLNAERLQSVGMIDELLDQVVLGSGLSDEHDIKVLSCVIIGRLASTCLS
KVIAAGRLHQITTRYTTLLGVVVKENAIKQEFEKHNEIVRNIERSSTQINDAISQAIS
KGDASNLPSDLELGEWESFYRTKVLKKSV"
gene complement(<66204..>66434)
/locus_tag="AWJ20_4623"
/db_xref="GeneID:30036776"
mRNA complement(<66204..>66434)
/locus_tag="AWJ20_4623"
/product="hypothetical protein"
/transcript_id="XM_018881706.1"
/db_xref="GeneID:30036776"
CDS complement(66204..66434)
/locus_tag="AWJ20_4623"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736157.1"
/db_xref="GeneID:30036776"
/translation="MQASRSYVIQASLDKLQDIDPDLRFMGFSDLNNEITNPDNAGLF
SADVQLTRNVINAILSKLEDPITEVQNQAMKW"
gene <69670..>71322
/locus_tag="AWJ20_4624"
/db_xref="GeneID:30036777"
mRNA <69670..>71322
/locus_tag="AWJ20_4624"
/product="hypothetical protein"
/transcript_id="XM_018881707.1"
/db_xref="GeneID:30036777"
CDS 69670..71322
/locus_tag="AWJ20_4624"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736158.1"
/db_xref="GeneID:30036777"
/translation="MTVASDVYNLSETYAEEPRPAKCIVIGAGVSGICFTYKAKQIME
NFDFVIYEKNPEVGGTWYESRYPGCSCDIPSHSYTYSWTANPNWSRLYVGSEEIFDYF
QGLAEKYGVYEKTKFETMVIGAKWLSDRDQWEVEVKDLKTGTVTKDYAEFLMNCGGLL
NNWKWPDIPGFHDFKGTKLHSAKWDKSVDVTNKRVAVIGSGSSAIQIVPSIQPSVSHL
VSFNRSPNWVTEEFSSELAFNGRETTYDEETRKKWAENREEFLKWRRRVEDTVNRGAE
LSLKGSELNEEVTSKARELMEKRLDYDPVLCKALIPEFPVGCKRITPGYLYLESLVEK
NVEVVSVGIKHVTENGIVDNNGKLHELDIIVAATGFDTTFTPRFPIIGLDGIDLRDKW
TKGPAAAYMSVAVPQLPNYFMTAGPNSPISNGSLIGTFETQIGYAMKLMQKVQKQGIR
YMVPKEDATREFNYHKDVAMQRYTYSSNCTSWYKSSKPGAPISGPWPGSSIHFRQIMQ
TPRYEDYDYAYQTDNRFQYLGIGRSRREYEGKDLGWYVAFDE"
gene <73083..>74557
/gene="PGK1"
/locus_tag="AWJ20_4625"
/db_xref="GeneID:30036778"
mRNA join(<73083..73152,73371..>74557)
/gene="PGK1"
/locus_tag="AWJ20_4625"
/product="phosphoglycerate kinase"
/transcript_id="XM_018881708.1"
/db_xref="GeneID:30036778"
CDS join(73083..73152,73371..74557)
/gene="PGK1"
/locus_tag="AWJ20_4625"
/inference="similar to AA sequence:KEGG_Orthology:K00927"
/note="3-phosphoglycerate kinase; catalyzes transfer of
high-energy phosphoryl groups from the acyl phosphate of
1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme
in glycolysis and gluconeogenesis; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16962558]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 16622836];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0016301 - kinase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0004618 - phosphoglycerate kinase
activity [Evidence IEA,IEA]; GO_function: GO:0004618 -
phosphoglycerate kinase activity [Evidence IDA] [PMID
6254992]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0006094 - gluconeogenesis
[Evidence IMP] [PMID 400791]; GO_process: GO:0006096 -
glycolytic process [Evidence IEA,IEA,IEA]; GO_process:
GO:0006096 - glycolytic process [Evidence IMP] [PMID
400791]; GO_process: GO:0016310 - phosphorylation
[Evidence IEA]"
/codon_start=1
/product="phosphoglycerate kinase"
/protein_id="XP_018736159.1"
/db_xref="GeneID:30036778"
/translation="MSLSNKLTIKDLDLKGKRVFIRVDVDFNVPLDGKTITNNQRIVA
ALPSIKYAKEQGAKAIILASHLGRPNGEVNEKYSLKPVADELSKLLDTKVAFLHDSVG
PEVEAYVNKADNGEIILLENVRFHIEEEGSAKKDGQKIKADPAKVKEFREQLTKLADV
YVNDAFGTSHRAHSSVVGLEVPQRAAGFLVSKELSFFAKALENPERPFLAILGGAKIS
DKIQLIDNLITKVNSLIIGGGMAFTFKKTLDNVEIGESLFDEAGSKVVASLVEKAKAN
NVELVFPVDYVTADKFDKDANVGSATDKSGIPSGWQGLDCGPESIKLYEAAISKAKTI
LWNGPPGVFEFEKFANGSKQTLNAVVKAAQNGSTVIIGGGDTATVAKKFGAEDKVSHV
STGGGASLELLEGKVLPGVAALSDKE"
gene <76568..>79138
/gene="GYP5"
/locus_tag="AWJ20_4626"
/db_xref="GeneID:30036779"
mRNA <76568..>79138
/gene="GYP5"
/locus_tag="AWJ20_4626"
/product="Gyp5p"
/transcript_id="XM_018881709.1"
/db_xref="GeneID:30036779"
CDS 76568..79138
/gene="GYP5"
/locus_tag="AWJ20_4626"
/note="GTPase-activating protein (GAP) for yeast Rab
family members; involved in ER to Golgi trafficking;
exhibits GAP activity toward Ypt1p that is stimulated by
Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p
and Rvs167p; involved in recruiting Rvs167p to the bud tip
during polarized growth; relocalizes from bud neck to
cytoplasm upon DNA replication stress; GYP5 has a paralog,
GYL1, that arose from the whole genome duplication;
GO_component: GO:0005798 - Golgi-associated vesicle
[Evidence IDA] [PMID 15331637]; GO_component: GO:0005933 -
cellular bud [Evidence IEA]; GO_component: GO:0005935 -
cellular bud neck [Evidence IEA]; GO_component: GO:0005935
- cellular bud neck [Evidence IDA] [PMID 15331637];
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 22842922]; GO_component: GO:0005934 - cellular
bud tip [Evidence IDA] [PMID 15331637]; GO_component:
GO:0005934 - cellular bud tip [Evidence IDA] [PMID
22842922]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 22842922]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 12189143]; GO_component: GO:0000131 -
incipient cellular bud site [Evidence IDA] [PMID
15331637]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 15331637]; GO_function: GO:0005096 -
GTPase activator activity [Evidence IEA]; GO_function:
GO:0005097 - Rab GTPase activator activity [Evidence IEA];
GO_function: GO:0005097 - Rab GTPase activator activity
[Evidence IGI,IPI,ISS] [PMID 12189143]; GO_function:
GO:0005097 - Rab GTPase activator activity [Evidence
IGI,IPI] [PMID 15802519]; GO_process: GO:0006888 - ER to
Golgi vesicle-mediated transport [Evidence IGI,IPI] [PMID
15802519]; GO_process: GO:0006887 - exocytosis [Evidence
IEA]; GO_process: GO:0043547 - positive regulation of
GTPase activity [Evidence IEA]; GO_process: GO:0032851 -
positive regulation of Rab GTPase activity [Evidence IEA];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:0032313 - regulation of Rab GTPase activity
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]; GO_process: GO:0016192 - vesicle-mediated
transport [Evidence IEA]; GO_process: GO:0016192 -
vesicle-mediated transport [Evidence IGI] [PMID 12189143]"
/codon_start=1
/product="Gyp5p"
/protein_id="XP_018736160.1"
/db_xref="GeneID:30036779"
/translation="MQHQNLESVSITDSSVDFAKSVIVTKTKEISSVEDDVCSIVDDA
SSESTRFDVCSENEKALASETPAVVQFKTQAETESEAEAEAEADSRSDSEFTLNSPSI
SVDLYQSEANDEANDDACDKGMKADGSAAVSAVSSARSRSGTMSEVASIASDTRSVAA
SFAPSVASASPGAIDWESLTDIEMRMVTESDSLKLIFARRDFLLVQEAEYMSDNEGDE
STETMARPDISCEEVREILLQAHSVIDSSSLVPGSQASATSLYPWKDIPMLKIADPSF
YDTNELALWSAIISDYRKTSVKVPKYTSMMVYSGIPPALRGRVWKAMANAYSPTLLSL
YDSLAAESTHFAKIIARDLNRTFPEISLFREKSGNGQAMLGRVMRAYSAYDIQVGYCQ
GLTFLVGPLLLHMNDRDAFCVMVRLMEDYDLRSMFTADMAGVPLRMYQFEYLLADILP
ELYEHFQKLGISTIYVSQWFLSMFAVTCPLSMLVRIFDLIFCEGAIPTLMRTSLAIMK
RNQAQLLEFDDEEPILELLLGKSLWEVYNLDADLLCSDITSIDAAQVLHGLEVAERGY
LSMGKLERARSRKNRGKIFGRGLNLTPKSPNKTPSSASASAQSSPSKLDKKPSRFFKW
TSAATETPVINYPVDEEDTTMITATNKRGHKTLSTASTIKRTLSQGSLSSGSTMSSNL
DDVFDSSNDDDSDDIDAQSAVVSTLDIKQPKSLSVSHSNPTLTRSPSELQLSQSLEIE
RKERQVDKDIFQSLLAQIPSEVIQSNPQLMSLSNELCQRLQISNDALNNTTPNTSCSS
CDRLLLDLATARTNEALARQELDLLQDEMAHLKCEYPSVPVPKPQPTIHKSPSKWNLW
"
gene <80554..>81120
/gene="VPS29"
/locus_tag="AWJ20_4627"
/db_xref="GeneID:30036780"
mRNA <80554..>81120
/gene="VPS29"
/locus_tag="AWJ20_4627"
/product="Vps29p"
/transcript_id="XM_018881710.1"
/db_xref="GeneID:30036780"
CDS 80554..81120
/gene="VPS29"
/locus_tag="AWJ20_4627"
/inference="similar to AA sequence:KEGG_Orthology:K18467"
/note="Subunit of the membrane-associated retromer
complex; endosomal protein; essential for
endosome-to-Golgi retrograde transport; forms a subcomplex
with Vps35p and Vps26p that selects cargo proteins for
endosome-to-Golgi retrieval; GO_component: GO:0005768 -
endosome [Evidence IPI] [PMID 9700157]; GO_component:
GO:0030904 - retromer complex [Evidence IMP,IPI] [PMID
9700157]; GO_component: GO:0030906 - retromer complex,
inner shell [Evidence IPI] [PMID 9700157]; GO_function:
GO:0008565 - protein transporter activity [Evidence
IMP,IPI] [PMID 9700157]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0042147 -
retrograde transport, endosome to Golgi [Evidence IPI]
[PMID 9700157]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Vps29p"
/protein_id="XP_018736161.1"
/db_xref="GeneID:30036780"
/translation="MSPSLPLNKVVTHGQLRIGFVAGHSVIPNSDPDALLITARQLDV
DMLIWGGSHRVEAYQLEGKFFISPGSATGAFTTGWPEEDVDDETDAANGADVDNTDDH
TTESNGTNSTKPPTSTDNDGQTTTTIDSDNKTGDNDDDADDDEHLDSTTPSFCLLDIQ
GSVCVLYIYSLIDGEVKVDKVSYRKDSP"
gene complement(<82014..>83429)
/gene="SHM2"
/locus_tag="AWJ20_4628"
/db_xref="GeneID:30036781"
mRNA complement(<82014..>83429)
/gene="SHM2"
/locus_tag="AWJ20_4628"
/product="glycine hydroxymethyltransferase SHM2"
/transcript_id="XM_018881711.1"
/db_xref="GeneID:30036781"
CDS complement(82014..83429)
/gene="SHM2"
/locus_tag="AWJ20_4628"
/inference="similar to AA sequence:KEGG_Orthology:K00600"
/note="Cytosolic serine hydroxymethyltransferase; converts
serine to glycine plus 5,10 methylenetetrahydrofolate;
major isoform involved in generating precursors for
purine, pyrimidine, amino acid, and lipid biosynthesis;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
12700234]; GO_component: GO:0005737 - cytoplasm [Evidence
IMP] [PMID 8132653]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 19053807];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 16622836]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0004372 - glycine
hydroxymethyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0004372 - glycine hydroxymethyltransferase
activity [Evidence IMP] [PMID 8132653]; GO_function:
GO:0004372 - glycine hydroxymethyltransferase activity
[Evidence IMP] [PMID 9398220]; GO_function: GO:0030170 -
pyridoxal phosphate binding [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0006563 - L-serine metabolic process
[Evidence IEA]; GO_process: GO:0006544 - glycine metabolic
process [Evidence IEA]; GO_process: GO:0006730 -
one-carbon metabolic process [Evidence IEA]; GO_process:
GO:0006730 - one-carbon metabolic process [Evidence IMP]
[PMID 8132653]; GO_process: GO:0006730 - one-carbon
metabolic process [Evidence IGI,IMP] [PMID 9398220];
GO_process: GO:0035999 - tetrahydrofolate interconversion
[Evidence IEA]"
/codon_start=1
/product="glycine hydroxymethyltransferase SHM2"
/protein_id="XP_018736162.1"
/db_xref="GeneID:30036781"
/translation="MSSYALSESHRHLLEEHVAEFDPEVAGIMKDEIARQRESIVLIA
SENFTSRAVFDALGSPMCNKYSEGYPGARYYGGNQHIDRIETLCQNRALEAFHVTADK
WGVNVQTLSGSPANLQVYQAIMKPHERLMGLDLPHGGHLSHGYQTDARKISAVSTYFE
TMPYRVDLETGLIDYDMLEKTAVLFRPKVLVAGTSAYCRLIDYKRMREIADKVGAYLV
VDMAHISGLIAAQVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSVNAKTGKEV
LYDLEGPINFSVFPGHQGGPHNHTITALAVALKQANTPEFVAYQKQVLKNAKALEEAF
KALNYTLVSNGTDSHMVLVSLRDKGIDGARIETVCELINIALNKNSIPGDKSALVPGG
VRIGAPAMSTRGLGEEDFKRIVSYIDAAVKYSIDVQSNLPKEANKLKDFKATVAKDED
GRLAKLKAEITAWAQEFPLPV"
gene complement(<84309..>85184)
/locus_tag="AWJ20_4629"
/db_xref="GeneID:30036782"
mRNA complement(<84309..>85184)
/locus_tag="AWJ20_4629"
/product="hypothetical protein"
/transcript_id="XM_018881712.1"
/db_xref="GeneID:30036782"
CDS complement(84309..85184)
/locus_tag="AWJ20_4629"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736163.1"
/db_xref="GeneID:30036782"
/translation="MGFSPNRIEAALKNAASDSLEDCIQWLEENEAKFEASEKFHQQL
NADTSTAASGDHSGEPAESSADGAAASETEPPKPLTEEEKAAKLELLRQKAAIRKAEQ
AKKDLEEQKKNEVIRRKHDQESIRVKEELKKKEAMQAAARRRQEAKEDALAKKRIKDL
IEADKRERLEKKQQLSSNPPPSLSSSSSSPSNAPTSSSTTSQPKPKPVYTESKLRFRI
QGIASAQPNLMKTFPIDATLGSVAETLATELAIPANSIVLTTTFPTRTLTSADFSLTL
KQADLVNASVILKQA"
gene <88864..>91776
/locus_tag="AWJ20_4630"
/db_xref="GeneID:30036784"
mRNA <88864..>91776
/locus_tag="AWJ20_4630"
/product="hypothetical protein"
/transcript_id="XM_018881714.1"
/db_xref="GeneID:30036784"
CDS 88864..91776
/locus_tag="AWJ20_4630"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736164.1"
/db_xref="GeneID:30036784"
/translation="MVLQNGAANGGVQGAAKVKLEQGPESTRPPVSGVSGRTPVVGGG
AVTTINSRHAGSVPGSGSVQSSGGDGGAGVIRSREISGSAAGSGPSSSGPGSGGLRTR
GRGGTTVSILNVDPETDGVAPQSFTVVSSLLQETYWGQNKSDEPDIGGPLGNSSGSAG
SNGSAGNGTSNGTDSGSAGNGVTIGDVNGNGSANGSASASTHGTGASNSISGSPVVTA
IGASTGGSTTHGVLSSNSTPGVSSAMDVDGATNGGGASKAVGGASIGILPDYNFQEYL
SYEPTDPPDEHTADTLINSFLAGSQGRFPYIDRNVLQQTHKSRFDPPPNMDCPRYRFA
SFTLYTAYAISSVSYTPPPGEHPEYEPFRYYATALHHAKLCKSDNIMWKITALLMCAM
FQMRTDKDKGCHYDMVRRAMKICVEVGLHKEVGLENMSKREQEMCRRLFWSVYSMERL
FAISTGRPFVMDEKEINIPFPCDMDDIDLLDEAKIIEHEKYRDSGEVRPKTSMTFIIY
IWTLRRLESDLVQAIYRVDRSIEERFSHVDFYLNKLKQWKESNRVTTPKEQNITNTAY
SRDIRLLLQPFLGRLSPDSDLFQMCVRETGHICQIFRDFYRKKESGFTTMAMHTNFVA
GLTLVYCLWLAKDSSILPILENVRSCTAALYVLTERSRLCMNYRDTFENLVSTTMKHI
IEHRRQKRQRREMEELEHQRRLEQQELEKQQEIERLHKEEEEHRKMRERIEQERIEMV
RQRMRQQQQQQQQQQQQQQQQQQQLQLVKQEPDDLAKSITTSPTPLDDNKHPPLTRIT
SALSTGSISSTSSTFPVIDRSPYLPSNNCATGIGGSVNGLPTTTPMPAPIQIAGPAIP
DTAASFAQLQTQGAPPPAITPLPGTLAFNGPSGMELQKDWIEAYGYDDTLYNMIQDIS
SWTKQVGDATSQAAAVSGAAIVANGTVAATSAETGGWFNDNSTDNAWMNISDFGYS"
gene <92091..>93134
/gene="STI1"
/locus_tag="AWJ20_4631"
/db_xref="GeneID:30036785"
mRNA <92091..>93134
/gene="STI1"
/locus_tag="AWJ20_4631"
/product="Hsp90 cochaperone STI1"
/transcript_id="XM_018881715.1"
/db_xref="GeneID:30036785"
CDS 92091..93134
/gene="STI1"
/locus_tag="AWJ20_4631"
/note="Hsp90 cochaperone; interacts with the Ssa group of
the cytosolic Hsp70 chaperones and activates Ssa1p ATPase
activity; interacts with Hsp90 chaperones and inhibits
their ATPase activity; homolog of mammalian Hop;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IPI] [PMID
12525481]; GO_function: GO:0042030 - ATPase inhibitor
activity [Evidence IDA] [PMID 21170051]; GO_function:
GO:0030544 - Hsp70 protein binding [Evidence IDA] [PMID
12716905]; GO_function: GO:0051879 - Hsp90 protein binding
[Evidence IDA] [PMID 12525481]; GO_function: GO:0051879 -
Hsp90 protein binding [Evidence IDA] [PMID 9927435];
GO_function: GO:0003729 - mRNA binding [Evidence IDA]
[PMID 21124907]; GO_process: GO:0006457 - protein folding
[Evidence IGI,IMP] [PMID 8972212]; GO_process: GO:0006950
- response to stress [Evidence IEA]"
/codon_start=1
/product="Hsp90 cochaperone STI1"
/protein_id="XP_018736165.1"
/db_xref="GeneID:30036785"
/translation="MAADAESFKAEGNSYYSQGLNQEAVDSYTKAIGISNSNPVYYSN
RAAAYMKLGRWQAALDDCNKGLSFSHDVKLGSKLYWRQGICHRELGSLSLAEESFNEG
LRRDPSNSVLREELARLQDILSSAPVAQTDSGLSEIKNNDSTASPINASESETTIPIQ
VVDTLPDIFSEPSAKVISHDDSNKTSHLHPLESIPTATDEKRNVQDIASPSASTSAQP
ALPLQIPSPLTFYNLMQLMRTPKSQLPQVYDHIFFNVPPSRYAEIHQTGGIDYEVIEF
VLDSIAFVATNAQDKNWASRAVDILEGLAHCNRFSMSKMFVSTKRVQEVQRLLAESKL
DASTLSRINDLYT"
gene <93618..>94421
/gene="YIH1"
/locus_tag="AWJ20_4632"
/db_xref="GeneID:30036786"
mRNA <93618..>94421
/gene="YIH1"
/locus_tag="AWJ20_4632"
/product="Yih1p"
/transcript_id="XM_018881716.1"
/db_xref="GeneID:30036786"
CDS 93618..94421
/gene="YIH1"
/locus_tag="AWJ20_4632"
/note="Negative regulator of eIF2 kinase Gcn2p; competes
with Gcn2p for binding to Gcn1p; may contribute to
regulation of translation in response to starvation via
regulation of Gcn2p; binds to monomeric actin and to
ribosomes and polyribosomes; ortholog of mammalian IMPACT;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005844 - polysome
[Evidence IDA] [PMID 22404850]; GO_component: GO:0005840 -
ribosome [Evidence IDA] [PMID 22404850]; GO_function:
GO:0003779 - actin binding [Evidence IEA]; GO_function:
GO:0003785 - actin monomer binding [Evidence IDA] [PMID
15126500]; GO_function: GO:0004860 - protein kinase
inhibitor activity [Evidence IMP] [PMID 15126500];
GO_function: GO:0043022 - ribosome binding [Evidence IDA]
[PMID 22404850]; GO_process: GO:0001933 - negative
regulation of protein phosphorylation [Evidence IMP] [PMID
15126500]; GO_process: GO:0006417 - regulation of
translation [Evidence IEA]"
/codon_start=1
/product="Yih1p"
/protein_id="XP_018736166.1"
/db_xref="GeneID:30036786"
/translation="MSNRSMSEIENEVEAINSIYEGCMSEWAPSIYDVHIPDSSVVVR
LSFPSVYPEKAPAVMAVVKGRQELSPQLESLISSLFVVGEVIVFDFIESCREFLEQEG
EGPDGADSGSYIDSPEASDVANGRDTGVDPTSSWIASEPVTDRKSLFIARVCRVESAQ
EAYDNIALLKTDKKISKATHNITAYRIKRSDGVSVQDCDDDGEDAAGGRLMHLLTLTD
SWNVVVCVSRWYGGIKLGPDRFKHINSTARDALVKGGFISISKNSSKKH"
gene <95708..>98530
/gene="PWP2"
/locus_tag="AWJ20_4633"
/db_xref="GeneID:30036787"
mRNA <95708..>98530
/gene="PWP2"
/locus_tag="AWJ20_4633"
/product="Pwp2p"
/transcript_id="XM_018881717.1"
/db_xref="GeneID:30036787"
CDS 95708..98530
/gene="PWP2"
/locus_tag="AWJ20_4633"
/inference="similar to AA sequence:KEGG_Orthology:K14558"
/note="Conserved 90S pre-ribosomal component; essential
for proper endonucleolytic cleavage of the 35 S rRNA
precursor at A0, A1, and A2 sites; contains eight
WD-repeats; PWP2 deletion leads to defects in cell cycle
and bud morphogenesis; GO_component: GO:0030686 - 90S
preribosome [Evidence IDA] [PMID 12150911]; GO_component:
GO:0030686 - 90S preribosome [Evidence IDA] [PMID
15231838]; GO_component: GO:0034388 - Pwp2p-containing
subcomplex of 90S preribosome [Evidence IDA] [PMID
15231838]; GO_component: GO:0034388 - Pwp2p-containing
subcomplex of 90S preribosome [Evidence IDA] [PMID
17515605]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 8804409]; GO_component: GO:0005730 - nucleolus
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 12068309]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 12150911]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0032040 - small-subunit processome
[Evidence IDA] [PMID 12068309]; GO_function: GO:0003729 -
mRNA binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0030515 - snoRNA binding [Evidence IPI] [PMID
12068309]; GO_process: GO:0007109 - cytokinesis,
completion of separation [Evidence IMP] [PMID 8804409];
GO_process: GO:0000480 - endonucleolytic cleavage in
5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15231838];
GO_process: GO:0000480 - endonucleolytic cleavage in
5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000480 - endonucleolytic cleavage in
5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15590835];
GO_process: GO:0000447 - endonucleolytic cleavage in ITS1
to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 15231838]; GO_process:
GO:0000447 - endonucleolytic cleavage in ITS1 to separate
SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic
rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 15489292]; GO_process: GO:0000447 -
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15590835]; GO_process: GO:0000472 - endonucleolytic
cleavage to generate mature 5'-end of SSU-rRNA from
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15231838]; GO_process: GO:0000472 - endonucleolytic
cleavage to generate mature 5'-end of SSU-rRNA from
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15489292]; GO_process: GO:0000472 - endonucleolytic
cleavage to generate mature 5'-end of SSU-rRNA from
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15590835]; GO_process: GO:0030010 - establishment of cell
polarity [Evidence IMP] [PMID 8804409]; GO_process:
GO:0000462 - maturation of SSU-rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 12068309]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA]"
/codon_start=1
/product="Pwp2p"
/protein_id="XP_018736167.1"
/db_xref="GeneID:30036787"
/translation="MSLDFKLSNLLGTVYRQGNLLFTPDGTSILSPVGNRVSCFDLVN
SSSFTFTYEHRKNISRIALNPQATLMLTVDEDGRGILVNFVRRTTIHHFNFKERVSDI
QFSPDGRHFAVAAGRHIQVWKTPNRNDDRQFAPFVRHRIYTGHYADVTSITWSNDSRF
FLTCSKDLTARVYSLIATDSSAATILAGHKDSVISAFFSQDQETIYTVSKDGALFEWK
YISKPKRLGGSTQSKEEAEDEEQDEETDLDANGNPIEYKFRWRIVNKHFFMQDNAKVR
CAAFHAPTNLLVVGFSTGIFGLYELPEFTNLQILNISQNDIDFVTINKTGEWLAFGAS
KLGQLLVWEWQSESYIIKQQGHYDAMNSLVYSPDGSKIVTASDDGKIKVWDAISGFAI
VTFTEHTSAVTSLQFSKKGSVLFSASLDGSIRAWDLIRYRNFRTFTSPHRIQFTSLAI
DPSGEVVCAGSLDDFDIHLWSVQTSQLLDRLSGHQGPVSSLCFGNEGGLLVSGSWDKT
VRIWSIFGRTSNVEPLQLQSEVMSVAMRPDSKRVAVATTDGQLSFWDPELGKQVGNID
GREDIAGGRHLTDRFSAANSQRSKHFTTLCYSADGSSILAGGNSKYIALYDVANEVLL
TRFTISRNMAIDGTVDQLNSKNMTESGPLGLIDTSGEASDLEDRIDNTLPGATRGDAS
VRKVRPAIRTTSIQFSPTGRSFAAASTEGLLLYSVDELLIFDPFDLDIDVTVDTTLEK
LAEKDYLIALVMAFRLGERSIIHQVYESIPFQDIELISRDLPQVYLERLLRFISSISE
ESPHIEFNLLWIQHLITYHGRFMVQRRHEFASAMRGLQKFVSRIGKDIIKLSRTNIYL
IRYLTGGVATIDSSLLEGMDVDSDEQENREVVPTNSMGVEHEEDEDEDEEDGWFGPES
RTEGINGKSATSGSDDEGVSEAEEDE"
gene <99978..>101096
/gene="CTR86"
/locus_tag="AWJ20_4634"
/db_xref="GeneID:30036788"
mRNA <99978..>101096
/gene="CTR86"
/locus_tag="AWJ20_4634"
/product="Ctr86p"
/transcript_id="XM_018881718.1"
/db_xref="GeneID:30036788"
CDS 99978..101096
/gene="CTR86"
/locus_tag="AWJ20_4634"
/inference="similar to AA sequence:KEGG_Orthology:K19323"
/note="Essential hypothetical protein; with orthologs in
Ashbya gossypii and Candida albicans; similar to human
ATXN10, mutations in which cause spinocerebellar ataxia
type 10; codon usage corresponds to that observed for
yeast genes expressed at low levels; relative distribution
to the nucleus increases upon DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 22842922]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22842922]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0008652 -
cellular amino acid biosynthetic process [Evidence IEA];
GO_process: GO:0009088 - threonine biosynthetic process
[Evidence IEA]"
/codon_start=1
/product="Ctr86p"
/protein_id="XP_018736168.1"
/db_xref="GeneID:30036788"
/translation="MCNEMTTSLKTEVLESPLEIVLKGSNTKFITAFKELLLHPVYSR
PTLQFIESSVKLAPNRARVLAESEHGRLIFIQLLKQTESWYSGEDNFYLHLLNSIISQ
LLSADLGNLFLSTFSEPIVNEDERLSLLKIVDAAIDSLLERKSTTLDCQPLLEILFRD
FTTASAQAIPFIRSADTSPEYSDSLLSQLTNIWNRLIVLLDIYQTILDNQPSLKPEIL
KDNIIEELVSLLGEAQKSLPRRSKLADLEKSASKSSSGNVSDHDDLDPKDFPLIKGKI
IYLLGTLVQENKQVQDLVRQLQGLELVLTNCIIDLNNPFIRERSILCLRYLLANNPEN
QDFVAKMEAKETVSEDALHEAGYETELVNGKLALKKRT"
gene complement(<101252..>103252)
/locus_tag="AWJ20_4635"
/db_xref="GeneID:30036789"
mRNA complement(<101252..>103252)
/locus_tag="AWJ20_4635"
/product="hypothetical protein"
/transcript_id="XM_018881719.1"
/db_xref="GeneID:30036789"
CDS complement(101252..103252)
/locus_tag="AWJ20_4635"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736169.1"
/db_xref="GeneID:30036789"
/translation="MSSSRTSRARHGRKRRLGNSQPSAGLRFTRISCAVIWCLLAGAP
IFGFSAFKQVLVANGVYREFCTPQEVEWWSEKGSHGPLLCTEQDYRLNSMFAYALAIS
GLLVLPVSAILDVYGPRVCGILASILLTFASLSLRAGTSITVGPYLDGYILGYILLGC
ASPPLFMSSLQLSTSFPKYGGSILVLLTAAMDGSSAVFLAFKLADRNGYIGSISQFFT
WYLFVPAFVFISQFVIMPSRGFKTVAELQRIIEVSSPFTLAGKSRGDEENNADDDGYS
YKWYRQQNNGHNGSRGDSTGAGNSYSFPEIASSQNEDSTPGEIDFTGAYRDNIDGNRA
TSGINVSSTVPRSESLSPNTSTINDGDDAEGENSPLLQHGDSSETFDDNDEYNNILAD
EEEAVANLNLVKDVTEVSGVWGALHNARVSQQLTSMWFILIILFSAVTTLHINYFIAS
IWKSYNYRLDGDTTSGSREVKLILDYFDFALPIGSILSVPFIGFILDNMSTLRAFTII
YVLAISIGFLQLVSGSVVAGCLGITLFVPYRSLLFTAIPDYTVNIFGFHTFGLVYGLI
LAVSGLANLLILQFNSGPLKMHHRLADPNSTNELLLGLTALVGLVLIMYIWLQGKKIR
RKQLEDEAESAPIQQMPEARITTNHDCRSGTCDDCVCEANVN"
gene complement(<104652..>106517)
/locus_tag="AWJ20_4636"
/db_xref="GeneID:30036790"
mRNA complement(<104652..>106517)
/locus_tag="AWJ20_4636"
/product="hypothetical protein"
/transcript_id="XM_018881720.1"
/db_xref="GeneID:30036790"
CDS complement(104652..106517)
/locus_tag="AWJ20_4636"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736170.1"
/db_xref="GeneID:30036790"
/translation="MTGDSSSDVLDVVNQLRAKENDLTFVASTVVPVFMSSRQRFEKL
QPEIIEAIVNTPVSTYTVPWIFALWAVLENESLSWRFVQLFRQFMTSFRADHARLAGF
ALAELLERIDSTGSSEYISPPYAIGIATGLRRGVGKLNHEVRGESDQRPLELSRLHSS
LAYWSVISKRYIFAKDMLAPTNRYVRPAKDYPISQIDVLFFHYYSSIVLIAEKRYFQA
AQYLTVVLNVPTVDERISSDLNRIQCDLRIASYAKWVCISLLHSGHVPSLSNLISDEL
VQSIRKHCEEYDTLSTLLRGNFHDKNMATSLEAIQEYINTQSDKFANDDNLDLVQLCF
EKYKRDYVRTLQTKVSSSMSIDYLDSRMGPLNPSQKQQHKGKLTLNTRTDSNCDHSNE
SHDNDNSDTGANNVSNSINESVYDYVNNLILSGQTDGEYKLLDAADKPPHLISTEPTS
KALSHMNQHMLQMIDSIINTAQDLDATMYDLVASGKFRSLLSKLSSKRPHLASVYAEF
NEESLGDGYGGLPSQFGLAARGSGQINIPGLEAAMFGRRRPDSMNQIQISSGTGRRRG
PGTIKSSARRRQAAPRMRQGSNHRSRHESTGSASSSEDAYDPMERDSENDDSTES"
gene <108419..>109786
/gene="PRO2"
/locus_tag="AWJ20_4637"
/db_xref="GeneID:30036791"
mRNA <108419..>109786
/gene="PRO2"
/locus_tag="AWJ20_4637"
/product="glutamate-5-semialdehyde dehydrogenase"
/transcript_id="XM_018881721.1"
/db_xref="GeneID:30036791"
CDS 108419..109786
/gene="PRO2"
/locus_tag="AWJ20_4637"
/inference="similar to AA sequence:KEGG_Orthology:K00147"
/note="Gamma-glutamyl phosphate reductase; catalyzes the
second step in proline biosynthesis; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0050661 - NADP
binding [Evidence IEA]; GO_function: GO:0004350 -
glutamate-5-semialdehyde dehydrogenase activity [Evidence
IEA,IEA]; GO_function: GO:0004350 -
glutamate-5-semialdehyde dehydrogenase activity [Evidence
IDA] [PMID 12513997]; GO_function: GO:0004350 -
glutamate-5-semialdehyde dehydrogenase activity [Evidence
IGI] [PMID 2824433]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0016620 - oxidoreductase activity, acting on the
aldehyde or oxo group of donors, NAD or NADP as acceptor
[Evidence IEA]; GO_process: GO:0055129 - L-proline
biosynthetic process [Evidence IEA]; GO_process:
GO:0008652 - cellular amino acid biosynthetic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0006561 - proline biosynthetic process
[Evidence IEA,IEA]; GO_process: GO:0006561 - proline
biosynthetic process [Evidence TAS] [PMID 11084050];
GO_process: GO:0006561 - proline biosynthetic process
[Evidence IDA] [PMID 12513997]"
/codon_start=1
/product="glutamate-5-semialdehyde dehydrogenase"
/protein_id="XP_018736171.1"
/db_xref="GeneID:30036791"
/translation="MAENLAKSARKASNILKTLSNDKRTSALKVIHDSLRDSKDEIIE
ANKLDLKLAEEADLPDSLIRRLDLAKGDKYDSMLQGILDVAALEDPVGRISLSRHLDD
GLDLYRVSCPVGVLLVIFEARPEVIANITALAIKSGNAAILKGGKESLHTFSAMATAV
KKALAKTSIPPDAIHLVSTREQVGELLHQDKYIDLVIPRGSNELVRQIKASTRIAVLG
HADGICSIYVHDDADGEMAGKIIVDSKVNYPAGCNAVETVLINKNLLSTSNPAGQAKV
TSIIEQLVDAKVKVKIAPEIEAFLTSGDNSSPSTIAIPCPQIEPATEADFDREFLSLT
IAIKSITDIDEAIAHINEHGSHHTDAIITASKDKAEKFLKGVDSAGVFWNASTRFADG
FRFGFGTEVGVSTNKLHARGPVGLEGLMSYQYQIKGSGQIVGDYAGSGGSKRYLHQDF
TVDNN"
gene complement(<109973..>112303)
/gene="DRS2"
/locus_tag="AWJ20_4638"
/db_xref="GeneID:30036792"
mRNA complement(<109973..>112303)
/gene="DRS2"
/locus_tag="AWJ20_4638"
/product="aminophospholipid-translocating P4-type ATPase
DRS2"
/transcript_id="XM_018881722.1"
/db_xref="GeneID:30036792"
CDS complement(109973..112303)
/gene="DRS2"
/locus_tag="AWJ20_4638"
/inference="similar to AA sequence:KEGG_Orthology:K14802"
/note="Trans-golgi network aminophospholipid translocase
(flippase); maintains membrane lipid asymmetry in
post-Golgi secretory vesicles; contributes to
clathrin-coated vesicle formation and endocytosis; subject
to auto-inhibition by its C-terminal tail; mutations in
human homolog ATP8B1 result in liver disease;
GO_component: GO:0005794 - Golgi apparatus [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005802 - trans-Golgi network
[Evidence IDA] [PMID 10601336]; GO_component: GO:0005802 -
trans-Golgi network [Evidence IDA] [PMID 12221123];
GO_component: GO:0005802 - trans-Golgi network [Evidence
IDA] [PMID 15090616]; GO_function: GO:0005524 - ATP
binding [Evidence IEA,IEA]; GO_function: GO:0019829 -
cation-transporting ATPase activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0000287 - magnesium ion binding
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_function:
GO:0004012 - phospholipid-translocating ATPase activity
[Evidence IEA,IEA]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IGI]
[PMID 15090616]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IMP]
[PMID 15249668]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence
IGI,IMP] [PMID 16452632]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IMP]
[PMID 8633245]; GO_process: GO:0006812 - cation transport
[Evidence IEA]; GO_process: GO:0032456 - endocytic
recycling [Evidence IMP] [PMID 24272750]; GO_process:
GO:0006897 - endocytosis [Evidence IGI] [PMID 12631737];
GO_process: GO:0006897 - endocytosis [Evidence IGI] [PMID
16195350]; GO_process: GO:0006886 - intracellular protein
transport [Evidence IGI] [PMID 12221123]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0045332 - phospholipid translocation [Evidence IMP]
[PMID 15249668]; GO_process: GO:0045332 - phospholipid
translocation [Evidence IMP] [PMID 16452632]; GO_process:
GO:0015914 - phospholipid transport [Evidence IEA];
GO_process: GO:0006892 - post-Golgi vesicle-mediated
transport [Evidence IGI,IMP] [PMID 10601336]; GO_process:
GO:0000028 - ribosomal small subunit assembly [Evidence
IMP] [PMID 8247005]"
/codon_start=1
/product="aminophospholipid-translocating P4-type ATPase
DRS2"
/protein_id="XP_018736172.1"
/db_xref="GeneID:30036792"
/translation="MYYKDTDTPAVCRTSSLVEELGQIEYIFSDKTGTLTRNIMEFKS
CSIAGRSYASEIPEDRKLTVVDGVEVGFRSFDAMIEDMDEPELGQVVQEFFTLLASCH
TVIPELKDNGKIKYQAASPDEGALVEGAASVGFKFTIRRPKFITVDIRGQDYEYELLN
ICEFNSTRKRMSTIFRCPDGKIRLYCKGADTVILERLAPENPFVTDTMAHLEEFAGEG
LRTLCLAMRVVPDDEYQEWARIFDEAATSMDNRAQKLDDAAELIERDLFLLGATAIED
KLQEGVPETIHTLQDAGIKIWVLTGDRQETAINIGMSCKLLSEDMNLLIINEENYEAV
RDNIAKKVAAIRGNQVSSAEYDTLALVIDGKSLGFALEPSLEKDFLDLAVICKAVICC
RVSPLQKALVVKLVKRHMKSLLLAIGDGANDVSMIQAAHVGVGISGMEGMQAARSADV
SIGQFKYLRKLLLVHGSWSYHRLSRAILYSFYKNIALYMTQFWYVFANGFSGQSIYES
WTITFYNVLCTVLPPFILGIFDQFISARLLDRYPQLYQLGQRSAFLNVRHFWEWVLNG
FYHSIILYIGSVYAYRYGQVLSNGLIADHWTWGTALFTACVLTTLAKAALVTNMWNKY
TVIAIPGSFFIWLGFFPAYATIAPLVNVSDEYRGVLPHLYPTLVFWATILILPCLCLI
RDFAWKYYKRMYKPESYHHVQEIQKYNIADYRPRMEQFQKAIRKVRQVQRMRKQRGFA
FSQADEGAGQATRVVRAYDTTQSRGQYGEMKPARQQ"
gene complement(<112477..>114270)
/gene="DRS2"
/locus_tag="AWJ20_4639"
/db_xref="GeneID:30036793"
mRNA complement(<112477..>114270)
/gene="DRS2"
/locus_tag="AWJ20_4639"
/product="aminophospholipid-translocating P4-type ATPase
DRS2"
/transcript_id="XM_018881723.1"
/db_xref="GeneID:30036793"
CDS complement(112477..114270)
/gene="DRS2"
/locus_tag="AWJ20_4639"
/inference="similar to AA sequence:KEGG_Orthology:K14802"
/note="Trans-golgi network aminophospholipid translocase
(flippase); maintains membrane lipid asymmetry in
post-Golgi secretory vesicles; contributes to
clathrin-coated vesicle formation and endocytosis; subject
to auto-inhibition by its C-terminal tail; mutations in
human homolog ATP8B1 result in liver disease;
GO_component: GO:0005794 - Golgi apparatus [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005802 - trans-Golgi network
[Evidence IDA] [PMID 10601336]; GO_component: GO:0005802 -
trans-Golgi network [Evidence IDA] [PMID 12221123];
GO_component: GO:0005802 - trans-Golgi network [Evidence
IDA] [PMID 15090616]; GO_function: GO:0005524 - ATP
binding [Evidence IEA,IEA]; GO_function: GO:0019829 -
cation-transporting ATPase activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0000287 - magnesium ion binding
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_function:
GO:0004012 - phospholipid-translocating ATPase activity
[Evidence IEA,IEA]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IGI]
[PMID 15090616]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IMP]
[PMID 15249668]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence
IGI,IMP] [PMID 16452632]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IMP]
[PMID 8633245]; GO_process: GO:0006812 - cation transport
[Evidence IEA]; GO_process: GO:0032456 - endocytic
recycling [Evidence IMP] [PMID 24272750]; GO_process:
GO:0006897 - endocytosis [Evidence IGI] [PMID 12631737];
GO_process: GO:0006897 - endocytosis [Evidence IGI] [PMID
16195350]; GO_process: GO:0006886 - intracellular protein
transport [Evidence IGI] [PMID 12221123]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0045332 - phospholipid translocation [Evidence IMP]
[PMID 15249668]; GO_process: GO:0045332 - phospholipid
translocation [Evidence IMP] [PMID 16452632]; GO_process:
GO:0015914 - phospholipid transport [Evidence IEA];
GO_process: GO:0006892 - post-Golgi vesicle-mediated
transport [Evidence IGI,IMP] [PMID 10601336]; GO_process:
GO:0000028 - ribosomal small subunit assembly [Evidence
IMP] [PMID 8247005]"
/codon_start=1
/product="aminophospholipid-translocating P4-type ATPase
DRS2"
/protein_id="XP_018736173.1"
/db_xref="GeneID:30036793"
/translation="MANYHGRGQAGNIPDEFSLIDHPSVPDPEPENPFDSRNASTTQI
GHTSTSAPLAGHDEDLDLFNGDSYHTNTTGNGGTRNGISASNGGIQQTSSNPGDWDYL
TPVDSSPYYDSPYNNASNPYLDGTGIVSGHDQDDFIMDSGSGIGSGSGRHDTIGLTHQ
ATGKHYGAKGGISGFYSNVRSKLGLNPAYTEMDLPLTEHATAGGAGRGGSGAATGIGS
GSRVGFSGGAGGSGSGGRGANEDRTETANDYDIRKFYYKLLGKKMPDASSLGPRIVTL
NDPAANQLGYKGNHISTTKYNAFTFIPKFLFEQFSKYANLFFLCTALIQQVPNVTPTN
RYTTIGTLAVVLLVSAFKELVEDVKRSNADKELNNSKVEVLNTNGEFETRRWVKLSVG
DIVRIKSEEPFPADILLLASSEPEGLCYIETANLDGETNLKIKQSRVETSTLVSTQDL
SRLRGRILSEQPNSSLYTYEATLELTGQDKMALAPDQLLLRGATLRNTPWIHGVVVFT
GHETKLMRNATAAPIKRTAVEHMINLQIIFLFSILLILAIVSSMGNVIKIKVTASTMW
YLQLEGTSLVKQFFQDLLTYWVLFSNLVPIR"
gene <121028..>122083
/gene="MNN10"
/locus_tag="AWJ20_4640"
/db_xref="GeneID:30036795"
mRNA <121028..>122083
/gene="MNN10"
/locus_tag="AWJ20_4640"
/product="Mnn10p"
/transcript_id="XM_018881725.1"
/db_xref="GeneID:30036795"
CDS 121028..122083
/gene="MNN10"
/locus_tag="AWJ20_4640"
/inference="similar to AA sequence:KEGG_Orthology:K05531"
/note="Subunit of a Golgi mannosyltransferase complex;
complex mediates elongation of the polysaccharide mannan
backbone; membrane protein of the mannosyltransferase
family; other members of the complex are Anp1p, Mnn9p,
Mnn11p, and Hoc1p; GO_component: GO:0005794 - Golgi
apparatus [Evidence IEA,IEA]; GO_component: GO:0000136 -
alpha-1,6-mannosyltransferase complex [Evidence IDA] [PMID
10037752]; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_function: GO:0000009 -
alpha-1,6-mannosyltransferase activity [Evidence IDA]
[PMID 10037752]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_function: GO:0016757 -
transferase activity, transferring glycosyl groups
[Evidence IEA]; GO_function: GO:0016758 - transferase
activity, transferring hexosyl groups [Evidence IEA];
GO_process: GO:0000917 - barrier septum assembly [Evidence
IMP] [PMID 16034811]; GO_process: GO:0000032 - cell wall
mannoprotein biosynthetic process [Evidence IMP] [PMID
10037752]; GO_process: GO:0006487 - protein N-linked
glycosylation [Evidence IMP] [PMID 23210626]; GO_process:
GO:0006487 - protein N-linked glycosylation [Evidence IMP]
[PMID 8991513]"
/codon_start=1
/product="Mnn10p"
/protein_id="XP_018736174.1"
/db_xref="GeneID:30036795"
/translation="MFKSEKGWKSRRPSSTSFWNFSGSSPMSPASSHNRVAAPAYRPL
LNFLLRRKILIVIFIFILLLSLFTAVPFLPHFSFGSANYVIILAANEGGGVMQWKGAR
EWSVERSSIANKKQYAERHGYNLAIKDVSAKKRYAHEWRESWEKVDIIKETMRQFPNA
EWFWWLDLHTYIMEPQISLDSHIFRNLYNETYRDASYFNPLSIPIEIPYVDYNQPIDL
VISQDCGGFNLGSFFIRRSEWTEKLLDIWWDPVFYEQKHMDWEHKEQDALETLYRNQA
WIRGRVGFIPLRKMNSFPPGACSDMADDSRLFYNEKERDFVVNMAGCEWGRDCWGEME
HYKALSKKLHQKKFWFF"
gene complement(<123523..>124443)
/gene="ENV9"
/locus_tag="AWJ20_4641"
/db_xref="GeneID:30036796"
mRNA complement(<123523..>124443)
/gene="ENV9"
/locus_tag="AWJ20_4641"
/product="Env9p"
/transcript_id="XM_018881726.1"
/db_xref="GeneID:30036796"
CDS complement(123523..124443)
/gene="ENV9"
/locus_tag="AWJ20_4641"
/note="Protein proposed to be involved in vacuolar
functions; mutant shows defect in CPY processing and
defects in vacuolar morphology; has similarity to
oxidoreductases, found in lipid particles; required for
replication of Brome mosaic virus in S. cerevisiae, a
model system for studying replication of positive-strand
RNA viruses in their natural hosts; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0005811 - lipid particle [Evidence
IEA,IEA]; GO_component: GO:0005811 - lipid particle
[Evidence IDA] [PMID 14562095]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence ISS] [PMID
8972580]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]; GO_process:
GO:0006624 - vacuolar protein processing [Evidence IMP]
[PMID 21912603]; GO_process: GO:0007033 - vacuole
organization [Evidence IMP] [PMID 21912603]"
/codon_start=1
/product="Env9p"
/protein_id="XP_018736175.1"
/db_xref="GeneID:30036796"
/translation="MVFFGPCWSVDSLPDFSGKSVFITGGNTGIGKHTVTELARKNMG
KIYLAGRNPTRCEAAIAEIKKEVPNANIEYVKLDVSKLDDVKQVAEKFVNEHTSLNYL
VNNAGIMATPYELNNDGYEMQFATNYMGHALLTRIMLPLLEKTAKDGDEVKIINVSSF
AHNFAPRVGINFEDVNLTKGSVWDRYGQSKLANILLNNELTKRYPQIKSYSLHPGFVR
TDLYDGMNSTYGILGRIANAVTKPFYITPYNGAITTLYCFSVEANDKAGEYLVPYGKV
GSLTRNAKNQELSSKLWDWTTNELTNKHYI"
gene <126168..>130211
/gene="SWI1"
/locus_tag="AWJ20_4642"
/db_xref="GeneID:30036797"
mRNA <126168..>130211
/gene="SWI1"
/locus_tag="AWJ20_4642"
/product="Swi1p"
/transcript_id="XM_018881727.1"
/db_xref="GeneID:30036797"
CDS 126168..130211
/gene="SWI1"
/locus_tag="AWJ20_4642"
/inference="similar to AA sequence:KEGG_Orthology:K11771"
/note="Subunit of the SWI/SNF chromatin remodeling
complex; regulates transcription by remodeling chromatin;
required for transcription of many genes, including ADH1,
ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+];
human homolog ARID1A is a candidate tumor suppressor gene
in breast cancer; GO_component: GO:0016514 - SWI/SNF
complex [Evidence IDA] [PMID 18644858]; GO_component:
GO:0016514 - SWI/SNF complex [Evidence IDA] [PMID
8016655]; GO_component: GO:0016514 - SWI/SNF complex
[Evidence IDA] [PMID 8127913]; GO_component: GO:0016514 -
SWI/SNF complex [Evidence IDA,IMP] [PMID 8159677];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
3143101]; GO_function: GO:0003677 - DNA binding [Evidence
IEA,IEA]; GO_function: GO:0015616 - DNA translocase
activity [Evidence IDA] [PMID 17188033]; GO_function:
GO:0001102 - RNA polymerase II activating transcription
factor binding [Evidence IPI] [PMID 11865042];
GO_function: GO:0001102 - RNA polymerase II activating
transcription factor binding [Evidence IMP,IPI] [PMID
14580348]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_process: GO:0043044 - ATP-dependent
chromatin remodeling [Evidence IDA] [PMID 8016655];
GO_process: GO:0006261 - DNA-dependent DNA replication
[Evidence IMP] [PMID 10198436]; GO_process: GO:0000436 -
carbon catabolite activation of transcription from RNA
polymerase II promoter [Evidence IGI] [PMID 14580348];
GO_process: GO:0044109 - cellular alcohol catabolic
process [Evidence IMP] [PMID 1339306]; GO_process:
GO:0031496 - positive regulation of mating type switching
[Evidence IMP] [PMID 6436497]; GO_process: GO:0045944 -
positive regulation of transcription from RNA polymerase
II promoter [Evidence IMP] [PMID 1339306]; GO_process:
GO:0045944 - positive regulation of transcription from RNA
polymerase II promoter [Evidence IMP] [PMID 3542227];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 8016655]; GO_process: GO:0061412 - positive
regulation of transcription from RNA polymerase II
promoter in response to amino acid starvation [Evidence
IMP] [PMID 10549298]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0005987 - sucrose catabolic process
[Evidence IMP] [PMID 1339306]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Swi1p"
/protein_id="XP_018736176.1"
/db_xref="GeneID:30036797"
/translation="MDPSSPATDVLQSQQGTIQPSALYSPSLFSNMSPQMENASPALS
NSGANSGGNPTNANNSNISMNSPFMGGISQGRSHSDAASVSPHQRPTPLPAQLLQEVH
SGSNSPMIGQQQGFQGQSQQQQQQANQMQNAGGRLSTGTQPDNGLGSDISQQFNGGND
LGLMYQQQMLGQQQQQTNQNQNQNRMFPNQNQASNQNQNQGLNQNQNSNQNLPLNFNP
NQGQNQNQHGPGSGPNQMPGQVPGQIPPGQVPGQGGLGNQGFLSTLTPAQLQPIRNQL
RLLSPQNQEEFIRRLGMLPPQNLIQAIQQLASQQQKMAAAQNSRNQAQARNQMNQRQP
NQQLNQLNNQQNQPNVPPGQQNQFQFVMQQNRQKSPQVNVNQPSQIPPNQKYPAGGPS
GPMGPNNSGTSGPGGLNMSSAAASTMSKADQFVKSLFEFMQRRGTPINSFPTVGESRV
HPFALYATVMKFGGSAKVTRAQQWPNVTQALGYMPSMSEEVSRIYNNILRPFEEFLLT
SQLRNKGLKANLPGQAGQVPMGQVPSPSPAQGPIKGPGMVPGQVAGPGSNQDSNQGPN
VPQTPFGQQAQPSPQVPQSQMPMSMIPMQMGQTPQTQIQQQQPPSGLRQSATPQPQTK
ARQTSQSRGKQTASPIPVHMPGPMQSPAVGGSPEVQAKLTPRRTPSSRKSSTASSPAL
VPVPALPAASPTPIIKPKHFVPHENRKYAPKKRLVEKHGGYDIRVLAGIGREIENIRS
DFPFTHELGTFDVHAITMAVNSTIAGEVRQALDKLMVITADPRCYFSLVESPGLLTGL
AECGLVITKGLASSKPDGKALATTKHYDVNDSLFGEGVKSNIDAVFESYKSKLGKEDV
DLVIDVDTLTGVEVGRVEDDKPTEKIQEDDISVHKKAEGQESKDTINEKASEQDGLKP
FGFTNYLTLLKKTSSENEELEFDYKTPEIDQFWVTASVDRLLSITTILRNLSFVDQNR
SILINEPTSREFLFGLLYSLAENANLLPNSKQTLDLMKDLVTIFSNLAPVLILDSAKD
ALVLLLFILAFTPTPIPLTTSKDGIVFGRYTPVVHRYLPYAVDILAKVLPRDPPNRDL
FESIFLGTCTNFEYLQLLQRYFNGRPADPYELMTCAFGLVISTVPHDDLSVIPRGLEL
ARPLLHQSLLVAEMLAEMIKFEKPTRSNTNGTVDADEDNDIEMSESDKSFGKEKEQNN
LSDFLKEFKEKKANYNLAYEWLSASDGFGHALLRAACALGAVVGNGRARDEERNPFAR
ITQRSITVLRILGKKAMEYDSQDGALTGSEKAIVQLPSGVLPTIEALLGAMLASEMDK
YVVGQICRFCDEGNEHLIKASSSLERP"
gene complement(<130280..>133468)
/gene="RAD1"
/locus_tag="AWJ20_4643"
/db_xref="GeneID:30036798"
mRNA complement(<130280..>133468)
/gene="RAD1"
/locus_tag="AWJ20_4643"
/product="ssDNA endodeoxyribonuclease RAD1"
/transcript_id="XM_018881728.1"
/db_xref="GeneID:30036798"
CDS complement(130280..133468)
/gene="RAD1"
/locus_tag="AWJ20_4643"
/inference="similar to AA sequence:KEGG_Orthology:K10848"
/codon_start=1
/product="ssDNA endodeoxyribonuclease RAD1"
/protein_id="XP_018736177.1"
/db_xref="GeneID:30036798"
/translation="MSEFEEDDENDLFVRQENDFYQSQSLIEAVENERNGTTVEGYTY
DGTNLSQAETPSHESKGPTNGLKTTTTTSGNHIITLHETEHESATLRDDIYNSEELFK
SVVLSTPLSFQKTIIRDMLADDALLVLGRGLGLHLIAANILHALDVAGTTTAKSIADE
STIENGTRQSRSSLVLLIGTNDIENSRISQELWQLSQLADPLTSCHRGLTIVSNETTT
LNQREKLYKRGGIFSVRYQALVVDMLSGAIDCSKVTGIVAFDSDRRVDKHYLEWVIRL
YRSKNTRGFIKALTENPEGFGTSGTLQPLAAKLKNLSIKRVLLWPRYHIEVTDSLLQT
GRGRKRNEIIEIQVEKSEAVRRIQTAIMDSIESCISRIKRTTASIKVISTEEWNLDNA
LSMKLSRQVRFELRPYWHLLSYDARHAVGDLAVLEQLLDDVLSLDSVAFLQRLIEISK
SATKSTRDVSQWLLEDAGSMLFQLAKERVYGKKSKDSTRPAIIEELPKWYHLMNILEE
ISAEKSNSNPDETGGPVLIMCKEKRTVTQLKAYIDTVGKAKNEPQNDNESIPPGQIYM
KERLQFYIDLNKMLLQKRRSCAPRGENLTGVASTGESATNTGNDSTSSNRAPFSRNKR
RRVRGGSVVASAAGRSVSPSLSLPEFEETIAEDTPIDADGLDDDIMRLISQDHVEEDK
DILHISEVFSRLQVMDSGQLVVIQQYDRFNDDSMLAELRPSYIIMYEPDTSFFRRIEV
TRASNPDRQIKAYFMFHKESVEEQRFLSEIRREKDAFTRLIREKANMAIHLETEKDTA
ARLTNNSTLLRNFSTRIAGGGQLGFNTNKPRVVVDVRELKAPLPAVLDFNNVEVVPMT
LTVGDYILSPEICVERKSITDFIQSMKSGRLHEQCTAMCRYYEFPAVLIEFDDLDSFS
FNSVGNSGPQPRSMGTADIDRFLSENDSLRTQISLLILQHPKLSLLWSPSPNDSAEIF
ARLKNERDEPDPALCARIGVSGGGNSIDYNIAAIDTLLAMPGITTNNYALITRKVPNI
RTLCSMSEEDISAIIGKEAAAKLYRFFNETVQ"
gene <134318..>136012
/gene="PEX29"
/locus_tag="AWJ20_4644"
/db_xref="GeneID:30036799"
mRNA <134318..>136012
/gene="PEX29"
/locus_tag="AWJ20_4644"
/product="Pex29p"
/transcript_id="XM_018881729.1"
/db_xref="GeneID:30036799"
CDS 134318..136012
/gene="PEX29"
/locus_tag="AWJ20_4644"
/note="Peroxisomal integral membrane peroxin; involved in
the regulation of peroxisomal size, number and
distribution; genetic interactions suggest that Pex28p and
Pex29p act at steps upstream of those mediated by Pex30p,
Pex31p, and Pex32p; forms ER foci upon DNA replication
stress; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005778 - peroxisomal membrane
[Evidence IEA]; GO_component: GO:0005778 - peroxisomal
membrane [Evidence IDA] [PMID 12707309]; GO_component:
GO:0005777 - peroxisome [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0007031 - peroxisome organization [Evidence IEA];
GO_process: GO:0007031 - peroxisome organization [Evidence
IDA,IGI,IMP] [PMID 12707309]"
/codon_start=1
/product="Pex29p"
/protein_id="XP_018736178.1"
/db_xref="GeneID:30036799"
/translation="MVSNSQDEKPLSDTLSSLWNNFINTSPNSSQSARDDESLFKSDS
DTQANAENTDIGIDMVKPEVQDDGSIGIVPATRNLGTASAVVSPVIAKTGSTEGTLLT
RKGDPIESLRSLPITNNEKSNSSERPRSSLLSMASASTSNWLNPIKPEKVLEMLFESW
AGSATQDLTTNRGRYQANLAPLSLPITFRNYNDFQSRCGIVYEMRYDIQAILEWKNPS
GTLSYLIVYSFACLHPRLFILCPFVYLLLGIMAPAYIAVHPGPRSGSLADMHSMPFSG
PPLHDPVIPKPVSQFSPEFLANVLDTQYAIGDAVKLFDKTVDWLSTFAYFTNEEWSSV
IYVAILFSTAIIYFSSPFLFTYIQWRYIFLSGGWVAMGTSYLKNVGIHRNMELFLAEF
KKSAMEKLPDSTDLSRYFEIKIPRLDVPRNFRFKEESELISKEVQIFEIQKFDVVESC
WQQSVFWGSPYAHMKEPCESLPDGQLPNKIESVLPPMDWNFETDAWERDYDPQEWVDN
HYLLQSRIFIDHDEKWVYDAEDGQKTNTYRRRRWSRVVTRTSDITPKPSTSLSKTE"
gene complement(<136122..>140093)
/gene="ADE6"
/locus_tag="AWJ20_4645"
/db_xref="GeneID:30036800"
mRNA complement(<136122..>140093)
/gene="ADE6"
/locus_tag="AWJ20_4645"
/product="phosphoribosylformylglycinamidine synthase"
/transcript_id="XM_018881730.1"
/db_xref="GeneID:30036800"
CDS complement(136122..140093)
/gene="ADE6"
/locus_tag="AWJ20_4645"
/inference="similar to AA sequence:KEGG_Orthology:K01952"
/note="Formylglycinamidine-ribonucleotide
(FGAM)-synthetase; catalyzes a step in the 'de novo'
purine nucleotide biosynthetic pathway; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0003824 - catalytic activity [Evidence
IEA]; GO_function: GO:0016874 - ligase activity [Evidence
IEA]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_function: GO:0004642 -
phosphoribosylformylglycinamidine synthase activity
[Evidence IEA,IEA]; GO_function: GO:0004642 -
phosphoribosylformylglycinamidine synthase activity
[Evidence IMP] [PMID 9331966]; GO_process: GO:0006189 -
'de novo' IMP biosynthetic process [Evidence IEA,IEA];
GO_process: GO:0006189 - 'de novo' IMP biosynthetic
process [Evidence IC] [PMID 9331966]; GO_process:
GO:0006541 - glutamine metabolic process [Evidence IEA];
GO_process: GO:0006164 - purine nucleotide biosynthetic
process [Evidence IEA]; GO_process: GO:0006164 - purine
nucleotide biosynthetic process [Evidence IMP] [PMID
1743513]"
/codon_start=1
/product="phosphoribosylformylglycinamidine synthase"
/protein_id="XP_018736179.1"
/db_xref="GeneID:30036800"
/translation="MMLTLPGPVAYSPFRVSGLINNINTAVNSSAVIGLRTVYVHYVS
VKDGSDLAKNNEKLTVLNSLLEYDSKPDVNEPLTKVLVDAITSENQQIPDNAYLLRVV
PRKGTISPWSSKATNILHNCGLVDDVERVERGLALIIQVRKGFPLDEHLKSGVFLDFI
YDRMTQSILERIPEKADFFATHEPKPLVSVPRNGIAAANKSLGLALDQPEIDYLTSSF
PNRDPTDVELFMFAQVNSEHCRHKIFNADWKIDGEAKPYSLFKMIRNTHEKNPKYTIS
AYSDNASVLEGHEATYYAPDPKTKEYQQIKETVHFLTKVETHNHPTAVSPFPGAATGS
GGEIRDEGAVGRGSKSKAGLSGYSVSDLLIPDFKQPWELDVGKPAHISSPLDIMLEAP
IGSAAFNNEFGRPAIGGYFRTLTTSVTNAAGKQEIRGFHKPIMLAGGMGSIRPQFALK
NRKITPGAALIVLGGPSMLIGLGGGAASSVSSGEGSVDLDFASVQRGNPEMQRRAQMV
IDACVALDGANPIQSIHDVGAGGLSNALTELVHDNDLGARFELRDVPNVEPGMSPMEI
WCCEAQERYVLGVSPSDLDTFKAICERERAIYAVVGYATSEQRLVLHDRLLDCTPIDM
EMSVLFGKPPKLSRVATTRQLKLEHFETSSISIKDAADRVLKLPAVGSKSFLITIGDR
SVTGLIAREQMVGPWQVPVADVAVTITSHGAGIHTGEAFGIGERPTVALINAGASAKL
SVAESLLNIFASDTTLERIRLSANWMAAPAHEGEGAALYEAVQAIGLDLCPELGIAIP
VGKDSMSMKMKWDKKEVTSPMSVVITAFGPVNDVRRTWTPQLERIEGSKLVLVDLGAG
KNRLGGSAVAQVYGQIGNEAPDVDAKLLKSFLHALETLHKKDDLVLAYHDRSDGGLLV
TALEMAFAGRVGLHLDVEDPSSDLVASLFNEELGAVFQVVDVEGFKAVLLEAGVPESV
ISVVGTVKSAKDQAISLRFNGQPAWEESRAALQQTWSLTSYKMQRLRDNPKAADQEFS
NIADNSDPGLSFKLTFENTPIPANLLTHKPKVAILREQGVNGHVEMAYAMQQAGFTPI
DVHMSDLMSKQVSLNEFVGFAACGGFSYGDVLGAGAGWAKSVLFHDNLKKEFSHFFND
RKDTFAIGVCNGCQFLSRIKSLLPGADNWPSFERNLSEQYEARTVVVEIVDDSAPGTE
PSIFLNSMRGSRFPVAVAHGEGYASFDSEDHLKKFSHDNLAVVRYVDNYGKVTEKYPF
NPNGSPEGITGIRTPNGRVLAMMPHPERVVIKEANSWYPKDLDFEDGPWLRLFRNARQ
WVN"
gene <140749..>141036
/locus_tag="AWJ20_4646"
/db_xref="GeneID:30036801"
mRNA <140749..>141036
/locus_tag="AWJ20_4646"
/product="hypothetical protein"
/transcript_id="XM_018881731.1"
/db_xref="GeneID:30036801"
CDS 140749..141036
/locus_tag="AWJ20_4646"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736180.1"
/db_xref="GeneID:30036801"
/translation="MVQTALKKSSSLGPTKSGKVSKHSKNPKKAAPKQIAPKKRGAVE
DKKIAKKHSAALTSATEKLLASRVGHLELLKGTRRELEKKEKEKKKKEAKK"
gene <142946..>143905
/gene="TWF1"
/locus_tag="AWJ20_4647"
/db_xref="GeneID:30036802"
mRNA <142946..>143905
/gene="TWF1"
/locus_tag="AWJ20_4647"
/product="Twf1p"
/transcript_id="XM_018881732.1"
/db_xref="GeneID:30036802"
CDS 142946..143905
/gene="TWF1"
/locus_tag="AWJ20_4647"
/inference="similar to AA sequence:KEGG_Orthology:K08870"
/note="Twinfilin; highly conserved actin
monomer-sequestering protein involved in regulation of the
cortical actin cytoskeleton; coordinates actin filament
severing and monomer sequestering at sites of rapid actin
turnover; composed of two cofilin-like regions, localizes
actin monomers to sites of rapid filament assembly;
GO_component: GO:0030479 - actin cortical patch [Evidence
TAS] [PMID 10652251]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005856 - cytoskeleton
[Evidence IEA,IEA]; GO_component: GO:0005622 -
intracellular [Evidence IEA]; GO_component: GO:0043332 -
mating projection tip [Evidence IDA] [PMID 19053807];
GO_function: GO:0003779 - actin binding [Evidence
IEA,IEA]; GO_function: GO:0051015 - actin filament binding
[Evidence IDA] [PMID 16569665]; GO_function: GO:0003785 -
actin monomer binding [Evidence IDA] [PMID 9700161];
GO_process: GO:0044396 - actin cortical patch organization
[Evidence IMP] [PMID 16569665]; GO_process: GO:0030042 -
actin filament depolymerization [Evidence IDA] [PMID
16569665]; GO_process: GO:0007015 - actin filament
organization [Evidence IDA,IMP] [PMID 16569665];
GO_process: GO:0051014 - actin filament severing [Evidence
IDA,IMP] [PMID 16569665]; GO_process: GO:0030837 -
negative regulation of actin filament polymerization
[Evidence IEA]; GO_process: GO:0042989 - sequestering of
actin monomers [Evidence IDA] [PMID 9700161]"
/codon_start=1
/product="Twf1p"
/protein_id="XP_018736181.1"
/db_xref="GeneID:30036802"
/translation="MANLAESCEPGKVIPKQSDRFEDDFDGIQRQLNAKEANYVAVKQ
NGPDAKFVFISFVPDAAPVRSKMLYASSSNTIYRELGGTQRFATSLFWTDLSEVSSSG
WNAHVKHEQATAPMTEEERSLQEVLHREVDQMIGTGERKNEIHRIQLSGSSRSGTGLS
LAVDDAVHEAFKQLHSAEPGSALGLHVDMDRESVIVGKPLSAVHADKLGDQFIDGSKP
QYTIYKHASPTGETDKIVFIYTCPSGSKIKERMVYASNRESVVSFARTHVDVIKVVEA
TDGSDISHTQLNDELYPESSETSSTSSTPRSGGFARPKRPGRR"
gene complement(<144144..>144980)
/gene="SFC1"
/locus_tag="AWJ20_4648"
/db_xref="GeneID:30036803"
mRNA complement(<144144..>144980)
/gene="SFC1"
/locus_tag="AWJ20_4648"
/product="Sfc1p"
/transcript_id="XM_018881733.1"
/db_xref="GeneID:30036803"
CDS complement(144144..144980)
/gene="SFC1"
/locus_tag="AWJ20_4648"
/inference="similar to AA sequence:KEGG_Orthology:K15100"
/note="Mitochondrial succinate-fumarate transporter;
transports succinate into and fumarate out of the
mitochondrion; required for ethanol and acetate
utilization; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IEA,IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence ISS] [PMID
7908717]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0005469 -
succinate:fumarate antiporter activity [Evidence IDA]
[PMID 9395087]; GO_process: GO:0015741 - fumarate
transport [Evidence IDA] [PMID 9395087]; GO_process:
GO:0015744 - succinate transport [Evidence IDA] [PMID
9395087]; GO_process: GO:0055085 - transmembrane transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Sfc1p"
/protein_id="XP_018736182.1"
/db_xref="GeneID:30036803"
/translation="MQLKRREVGPDGKKVKPVGFIRTGIRIVQKETPLGLYKGLGAVI
AGITPKMAIRFSTYEYLKEKLTPVDKPISTLNTFVAGVGAGLTEAVLIVNPTEVVKIR
LQAQAHSLVDPVTGPKYRNAAHCVYTVIREEGIGAMYRGVVLTAARQASNQGVNFTVY
SGLKAKLQELQPEYQMLPSWQTSIIGLISGALGPLSNAPLDTIKTRMQREGGHSTESG
LARFTRIAKQLIQQEGTAALYRGITPRIMRVAPGQAVTFTIYEMARGQLNKIPIFNQT
PA"
gene <150734..>152857
/gene="RSC9"
/locus_tag="AWJ20_4649"
/db_xref="GeneID:30036804"
mRNA <150734..>152857
/gene="RSC9"
/locus_tag="AWJ20_4649"
/product="Rsc9p"
/transcript_id="XM_018881734.1"
/db_xref="GeneID:30036804"
CDS 150734..152857
/gene="RSC9"
/locus_tag="AWJ20_4649"
/inference="similar to AA sequence:KEGG_Orthology:K11763"
/note="Component of the RSC chromatin remodeling complex;
DNA-binding protein involved in the synthesis of rRNA and
in transcriptional repression and activation of genes
regulated by the Target of Rapamycin (TOR) pathway;
GO_component: GO:0016586 - RSC complex [Evidence IDA]
[PMID 10619019]; GO_component: GO:0016586 - RSC complex
[Evidence IDA] [PMID 11931764]; GO_component: GO:0016586 -
RSC complex [Evidence IDA] [PMID 12183366]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence IEA]; GO_function:
GO:0015616 - DNA translocase activity [Evidence IDA] [PMID
12183366]; GO_function: GO:0015616 - DNA translocase
activity [Evidence IDA] [PMID 16455496]; GO_function:
GO:0015616 - DNA translocase activity [Evidence IDA] [PMID
17188033]; GO_function: GO:0015616 - DNA translocase
activity [Evidence IDA] [PMID 17918861]; GO_function:
GO:0003682 - chromatin binding [Evidence IPI] [PMID
11931764]; GO_process: GO:0043044 - ATP-dependent
chromatin remodeling [Evidence IDA,IDA] [PMID 12183366];
GO_process: GO:0000086 - G2/M transition of mitotic cell
cycle [Evidence IMP] [PMID 17542652]; GO_process:
GO:0070301 - cellular response to hydrogen peroxide
[Evidence IDA] [PMID 11931764]; GO_process: GO:0016568 -
chromatin modification [Evidence IEA]; GO_process:
GO:0006338 - chromatin remodeling [Evidence IPI] [PMID
11931764]; GO_process: GO:0006337 - nucleosome disassembly
[Evidence IDA] [PMID 16492771]; GO_process: GO:0006364 -
rRNA processing [Evidence IEA]; GO_process: GO:0009303 -
rRNA transcription [Evidence IMP] [PMID 11931764];
GO_process: GO:0006357 - regulation of transcription from
RNA polymerase II promoter [Evidence IMP] [PMID 11931764];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA,IEA]; GO_process: GO:0006368 -
transcription elongation from RNA polymerase II promoter
[Evidence IDA] [PMID 17081996]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0061587 - transfer RNA gene-mediated silencing
[Evidence IMP] [PMID 23707796]"
/codon_start=1
/product="Rsc9p"
/protein_id="XP_018736183.1"
/db_xref="GeneID:30036804"
/translation="MSSPGGRVLRHHQTRPNYGSSSTPNTRISGISGPSKTPGGISTP
GTNNIPVHVRGGTVMSGLTVSNGLTPSTPGTPGTPSPSTPGLLGSAHSVTPQPTGDVP
AHMIPPASMAVYTPQNAPHLYQSTVYQRINPSQQPYIGMGMRGVDFIVRISMSLQCGI
EDEVSWALKALLELSVRQPGLIQFKNNTSIPLLLFDKIARSSIFAAPVSSTILNDSTS
APGPGLDSEVDSFTPSNTADDLQVGISSDIGGNTTVTTGNGPESSTDSAPITVPDLNS
PFYGSADSDFFQLHKTIEALLILRNASLDPENAHFLANSPFCNEIVLRGLQFMIAGND
PKRPNSTTSSNPAVAEISSYCIDIAESISFHMTPDSPSNPLFATICKIISASTDRSTL
IPTIRCLSRLMIRDDKNIVGSVPLPIVSSILRYLLVTDDEELMSASLDFLYQYTARRE
NVSRLINISDVENGVNNKDAHLNLEIVTTHLSRLLTYKIEPQPVADYIRLPRKTKEPV
PTEPPKLPAWILKELYAMEEPDRATHWIRTCYIDDEDGEVTQISLWKAYEAQFETFAR
ESGRRLLPAVDFIKNVTSAFRTSSAMVVQLPGGQKKFIIKGIRPREYPIAPSVLNAPK
QPDPKPTDQANSIVREPSAVCVTSALVLQNIARSPAGRTLLRPRVEEFIGSYTVNLAL
GSYIDGLLDIVSRSGEHDLDDEDHK"
gene <155378..>155926
/locus_tag="AWJ20_4650"
/db_xref="GeneID:30036806"
mRNA <155378..>155926
/locus_tag="AWJ20_4650"
/product="hypothetical protein"
/transcript_id="XM_018881736.1"
/db_xref="GeneID:30036806"
CDS 155378..155926
/locus_tag="AWJ20_4650"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736184.1"
/db_xref="GeneID:30036806"
/translation="MKFSTIAIATVSAATVVNASYSYSPQVNDFLNKIDIAKAQVYDQ
AGSLRDDLIDSWSDSQLKEWLDAHSLYDVHTSATKDVKGYAASLKEQVLAHKDVLVED
IKSYQAAAAANAQPYIGKSKEFVADTGAKAGEVGAQAQVHAQTLLGHVQQHLYTQYEN
VKSYAWDLYKRATAYSKSKVDL"
gene complement(<158555..>159265)
/locus_tag="AWJ20_4651"
/db_xref="GeneID:30036807"
mRNA complement(<158555..>159265)
/locus_tag="AWJ20_4651"
/product="hypothetical protein"
/transcript_id="XM_018881737.1"
/db_xref="GeneID:30036807"
CDS complement(158555..159265)
/locus_tag="AWJ20_4651"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736185.1"
/db_xref="GeneID:30036807"
/translation="MFESKQLNSRDLWTACALSGKLLRDPIVSDYKGRLYNKESILEY
LLTPEKFGASQRAKVTYITGIKDIVELKITRESKSQTWICPLTEKDIVKESASQRFVY
IAECGHLMTEKAIRQMTMVDNAKDHGNGTANGSSAESSLNHGDRDNKKEYCPVCDTEY
IPVNLVTVNPSIPELVKKNEDRMKLLAELGLTHSLKPKKKKEKEKKKQKSSTEKKRKS
LTTSDDVPAKKTKVPVDT"
gene <160555..>162177
/locus_tag="AWJ20_4652"
/db_xref="GeneID:30036808"
mRNA <160555..>162177
/locus_tag="AWJ20_4652"
/product="hypothetical protein"
/transcript_id="XM_018881738.1"
/db_xref="GeneID:30036808"
CDS 160555..162177
/locus_tag="AWJ20_4652"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736186.1"
/db_xref="GeneID:30036808"
/translation="MILCQFPRLMALLLPLLALIYGETNTCPAQTCLVSSSGGKFNGI
CQASFSEFLGIPYAQPPTGNLRFANAVQYTPNSSPRNAKTLPPYCPQTSTTVTSTHED
CLYLNVYTPSTKASNEVLFWIHGGSFIQGGSADPVFNGSQLAQAENIVVVTVNYRLGA
LGFYDSPETGTNFGLTDVVMALKWVNENIASYGGKPDSVTIAGESSGATVVRTLLMTP
SAKGLFSRAIIQSDPQAYSANNSTVSQDGIAPIIDQSLGCSTNSGEDLITCLRNASVS
DILSAQMNVYSELTNIPGVNSVYPFGPNVNSENLPMDLTPALTSQSPIVNPVEVMIGV
VKDEGLAAVSQVFGSAGMTPDIYQYALAAQLGPVGAGKILSSSLFSTDINSSTYPQGI
NYELQLSSIATEYEFSCPSQMTAQLYQTSSHKYTYFYQFVEGIQYPDNSGFALCSNGA
VCHEDDLYLVFGTYPNPTSLSSSQTSLVKEVQTRWGSFVKNGNPNCNGYSNWTPSSGQ
NLNVQLLGGQGSISSIDQAQCQFLDTQLGYYW"
gene complement(<162426..>162887)
/gene="RCF1"
/locus_tag="AWJ20_4653"
/db_xref="GeneID:30036809"
mRNA complement(<162426..>162887)
/gene="RCF1"
/locus_tag="AWJ20_4653"
/product="Rcf1p"
/transcript_id="XM_018881739.1"
/db_xref="GeneID:30036809"
CDS complement(162426..162887)
/gene="RCF1"
/locus_tag="AWJ20_4653"
/note="Cytochrome c oxidase subunit; required for assembly
of the Complex III-Complex IV supercomplex, and for
assembly of Cox13p and Rcf2p into cytochrome c oxidase;
similar to Rcf2p, and either Rcf1p or Rcf2p is required
for late-stage assembly of the Cox12p and Cox13p subunits
and for cytochrome c oxidase activity; required for growth
under hypoxic conditions; member of the hypoxia induced
gene family; C. elegans and human orthologs are functional
in yeast; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA]; GO_component: GO:0031305 -
integral component of mitochondrial inner membrane
[Evidence IDA] [PMID 22310663]; GO_component: GO:0031305 -
integral component of mitochondrial inner membrane
[Evidence IDA] [PMID 22342701]; GO_component: GO:0031305 -
integral component of mitochondrial inner membrane
[Evidence IDA] [PMID 22405070]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0031966 -
mitochondrial membrane [Evidence IEA]; GO_component:
GO:0097249 - mitochondrial respiratory chain supercomplex
[Evidence IDA] [PMID 22310663]; GO_component: GO:0097249 -
mitochondrial respiratory chain supercomplex [Evidence
IDA] [PMID 22342701]; GO_component: GO:0097249 -
mitochondrial respiratory chain supercomplex [Evidence
IDA] [PMID 22405070]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14562095];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0033617 -
mitochondrial respiratory chain complex IV assembly
[Evidence IGI] [PMID 22310663]; GO_process: GO:0097250 -
mitochondrial respiratory chain supercomplex assembly
[Evidence IMP] [PMID 22342701]; GO_process: GO:0097250 -
mitochondrial respiratory chain supercomplex assembly
[Evidence IMP] [PMID 22405070]"
/codon_start=1
/product="Rcf1p"
/protein_id="XP_018736187.1"
/db_xref="GeneID:30036809"
/translation="MSSVPPNSDYTSLTGEEDILDKLARKAKEQPVVPIGMLLTCGAL
YMSARALRGGNSKLANRMFYWRVGLQGFTVAALVIGGYYYGKPNRPTDRDSILRRTAK
EREDIWIKELERVDEMDKERKARADAVALDKKERLRRQFEEARAASGKKDE"
gene complement(<163421..>163678)
/gene="GUK1"
/locus_tag="AWJ20_4654"
/db_xref="GeneID:30036810"
mRNA complement(<163421..>163678)
/gene="GUK1"
/locus_tag="AWJ20_4654"
/product="guanylate kinase"
/transcript_id="XM_018881740.1"
/db_xref="GeneID:30036810"
CDS complement(163421..163678)
/gene="GUK1"
/locus_tag="AWJ20_4654"
/inference="similar to AA sequence:KEGG_Orthology:K00942"
/note="Guanylate kinase; converts GMP to GDP; required for
growth and mannose outer chain elongation of cell wall
N-linked glycoproteins; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0004385 - guanylate kinase activity
[Evidence IEA,IEA]; GO_function: GO:0004385 - guanylate
kinase activity [Evidence IDA] [PMID 1334480];
GO_function: GO:0016301 - kinase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0046037 - GMP metabolic
process [Evidence TAS] [PMID 11035032]; GO_process:
GO:0046939 - nucleotide phosphorylation [Evidence
IEA,IEA]; GO_process: GO:0016310 - phosphorylation
[Evidence IEA]; GO_process: GO:0006163 - purine nucleotide
metabolic process [Evidence IEA]"
/codon_start=1
/product="guanylate kinase"
/protein_id="XP_018736188.1"
/db_xref="GeneID:30036810"
/translation="MQGVKSVKKTDLNARFLFIAPPSVETLKSRLEGRGTETQESLNN
RLNQALAELEYSKEPGAHDKIIVNDDLEKAYAELKAFVTSE"
gene <164583..>165614
/locus_tag="AWJ20_4655"
/db_xref="GeneID:30036811"
mRNA <164583..>165614
/locus_tag="AWJ20_4655"
/product="hypothetical protein"
/transcript_id="XM_018881741.1"
/db_xref="GeneID:30036811"
CDS 164583..165614
/locus_tag="AWJ20_4655"
/inference="similar to AA sequence:KEGG_Orthology:K08502"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736189.1"
/db_xref="GeneID:30036811"
/translation="MNISIPGYSETSPTYFNIQISFDRTTYHVKRRYSEFVKFVEAIE
EEMGEQAPVDLPGKKWIGTKNREFLDDRRRALELFLRALAKKDEWRESLAFMDFLEVS
KHAKSESSVAAKARGDWLKMESEASSLIQKALKSKQLMASGIGGPTEAAEHRRLVVLS
RSKIKHLESVLSSDNSLGDGEYRRRRDIVQNLKLALRNGENTSSSPFGSNMSSGAGNG
ISSAFNQTSSLSGASGTGVSSPLSSRSPSRTSTPTRILGAPAPETGRTRQLNNSGLLA
LQKSDMEEQDRTVESLRQTIEQQKRLGLAINEELTFQNQLLDELDDETHRVNSRLNQA
KRRTGNLAN"
gene <168708..>170060
/gene="CSR1"
/locus_tag="AWJ20_4656"
/db_xref="GeneID:30036812"
mRNA <168708..>170060
/gene="CSR1"
/locus_tag="AWJ20_4656"
/product="Csr1p"
/transcript_id="XM_018881742.1"
/db_xref="GeneID:30036812"
CDS 168708..170060
/gene="CSR1"
/locus_tag="AWJ20_4656"
/note="Phosphatidylinositol transfer protein; has a
potential role in regulating lipid and fatty acid
metabolism under heme-depleted conditions; interacts
specifically with thioredoxin peroxidase; may have a role
in oxidative stress resistance; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
12869188]; GO_component: GO:0005768 - endosome [Evidence
IEA,IEA]; GO_component: GO:0005768 - endosome [Evidence
IDA] [PMID 10848624]; GO_component: GO:0005768 - endosome
[Evidence IDA] [PMID 12869188]; GO_component: GO:0005811 -
lipid particle [Evidence IDA] [PMID 17803462];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005628 -
prospore membrane [Evidence IDA] [PMID 24390141];
GO_function: GO:0008525 - phosphatidylcholine transporter
activity [Evidence IDA] [PMID 10848624]; GO_function:
GO:0008526 - phosphatidylinositol transporter activity
[Evidence IDA] [PMID 10848624]; GO_process: GO:0043001 -
Golgi to plasma membrane protein transport [Evidence IGI]
[PMID 10848624]; GO_process: GO:0006893 - Golgi to plasma
membrane transport [Evidence IGI] [PMID 16126894];
GO_process: GO:0016042 - lipid catabolic process [Evidence
IEA]; GO_process: GO:0006629 - lipid metabolic process
[Evidence IEA]; GO_process: GO:0006869 - lipid transport
[Evidence IEA]; GO_process: GO:0045717 - negative
regulation of fatty acid biosynthetic process [Evidence
IDA,IGI,IMP] [PMID 17803462]; GO_process: GO:1901352 -
negative regulation of phosphatidylglycerol biosynthetic
process [Evidence IGI] [PMID 12869188]; GO_process:
GO:0046488 - phosphatidylinositol metabolic process
[Evidence IGI] [PMID 16262726]; GO_process: GO:0009395 -
phospholipid catabolic process [Evidence IEA]; GO_process:
GO:0015914 - phospholipid transport [Evidence IDA] [PMID
10848624]; GO_process: GO:2001247 - positive regulation of
phosphatidylcholine biosynthetic process [Evidence IGI]
[PMID 12869188]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Csr1p"
/protein_id="XP_018736190.1"
/db_xref="GeneID:30036812"
/translation="MSTAAKGTLPGRAGNLTTEQEHKLKEFWAQVLIYTGSAPESLAT
QLTSYSVTSGAAAKKKGGFFSKKDKHVDDIATQSELFRAALPKLTPETFIKAVRFMSR
GDNVDDLFLRFLRARKWDVAKALEMFATTISWRVEFGVDELLRTGEEQCVKDKEDGVI
LQFKTGKAILRGKDKTGRPIVDVHVKLHDPKAQSERDIEKFTIFLIEASRLCLNDPND
TAAVIFDLTDFAISNMDYAAVKFIITCFERHYPECLGFLFIHNAPWVFQGIWNVIKGW
IDPVVASKISFTRNHNDLVKLIDDKEIPKSMGGSRPWDFEYLPPVPGENQKLEDHETR
DKILAERVNLFKELEQVTVQWISATGEQSNALEAKRSQIIAEVRKNYFLLDPYVRARS
AYDRDGTLAEFKQQLHQPELEAAAQAAKTGKAPSLADEKNDQTRVKTAPAAAPAAVTA
"
gene <172055..>172846
/locus_tag="AWJ20_4657"
/db_xref="GeneID:30036813"
mRNA <172055..>172846
/locus_tag="AWJ20_4657"
/product="hypothetical protein"
/transcript_id="XM_018881743.1"
/db_xref="GeneID:30036813"
CDS 172055..172846
/locus_tag="AWJ20_4657"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736191.1"
/db_xref="GeneID:30036813"
/translation="MNWNIEYKPLVEQDARVFGGLDRDMVQQTLQMVVSFVDNFGLSL
LELTNDDCVVQDSILSFYENLPKVCLVRITPKIVIIGYEMAARLVFSQSPFATSRVCG
VIASYREICTNSKNRDDPAVSTFNSLVMDTCNILWRDNAFSESVNSDQFGLTKEFIEA
LDKQCQEKSLSFKALYSITNSPLFIWVSMLYLRLKEHENFAATEVYTGQITRERFQEM
SKAGNWLDIEYDDFRVGLLSKLEDAGYEGINVLLYTSLKQLMDRR"
gene <174040..>174744
/gene="MCH5"
/locus_tag="AWJ20_4658"
/db_xref="GeneID:30036814"
mRNA <174040..>174744
/gene="MCH5"
/locus_tag="AWJ20_4658"
/product="Mch5p"
/transcript_id="XM_018881744.1"
/db_xref="GeneID:30036814"
CDS 174040..174744
/gene="MCH5"
/locus_tag="AWJ20_4658"
/note="Plasma membrane riboflavin transporter; facilitates
the uptake of vitamin B2; required for FAD-dependent
processes; sequence similarity to mammalian
monocarboxylate permeases, however mutants are not
deficient in monocarboxylate transport; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 16204239]; GO_function: GO:0008028 - monocarboxylic
acid transmembrane transporter activity [Evidence IGI,IMP]
[PMID 11536335]; GO_function: GO:0032217 - riboflavin
transporter activity [Evidence IGI] [PMID 16204239];
GO_process: GO:0032218 - riboflavin transport [Evidence
IGI] [PMID 16204239]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Mch5p"
/protein_id="XP_018736192.1"
/db_xref="GeneID:30036814"
/translation="MSEKKNAESVMKPQCDENYADGGVGVGAQFDIDSQSVRSSGINV
EVINNSGDEEKTVGDKPIPEAVPVVGPPPNGGLQAYLAVLGGFFALFNSWGLINTFGA
FETYYQEDLLSHEDPSTIAWIGSIQGYFVVAAAIVFGRMVDAGYIQSLLYVGTFLLTF
GMMMTSISSEFYQIFLAQGVVVGIGCCCMFVVSVSVVASYFSTRRAFFVGIVASGVSL
MVVNDGVREIITRLAG"
gene <174814..>175548
/gene="MCH5"
/locus_tag="AWJ20_4659"
/db_xref="GeneID:30036815"
mRNA <174814..>175548
/gene="MCH5"
/locus_tag="AWJ20_4659"
/product="Mch5p"
/transcript_id="XM_018881745.1"
/db_xref="GeneID:30036815"
CDS 174814..175548
/gene="MCH5"
/locus_tag="AWJ20_4659"
/note="Plasma membrane riboflavin transporter; facilitates
the uptake of vitamin B2; required for FAD-dependent
processes; sequence similarity to mammalian
monocarboxylate permeases, however mutants are not
deficient in monocarboxylate transport; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 16204239]; GO_function: GO:0008028 - monocarboxylic
acid transmembrane transporter activity [Evidence IGI,IMP]
[PMID 11536335]; GO_function: GO:0032217 - riboflavin
transporter activity [Evidence IGI] [PMID 16204239];
GO_process: GO:0032218 - riboflavin transport [Evidence
IGI] [PMID 16204239]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Mch5p"
/protein_id="XP_018736193.1"
/db_xref="GeneID:30036815"
/translation="MVRELITRIGFGWATRVVAFTIFGLSILPCLFLKGRLPPRKSGP
LIDYASFKDIPFILYCIACFFGFMGQYIPIFFIQSYCLEIGIDSNLAFYMTSILNAGS
VLGRILPNFIADKTGPLNMLLPCTVACAILAYCWDFISVKGGVIAFSVLFGFFSGTFV
SLPPACIASMTAEMNMLGTRMGMAFFLCGFGILIGSPIGGALVTRDDGAFLYAAMFCA
ACMTLSSVFMFMTRVALSGPKVMVKC"
gene complement(<177061..>177663)
/gene="PMU1"
/locus_tag="AWJ20_4660"
/db_xref="GeneID:30036817"
mRNA complement(<177061..>177663)
/gene="PMU1"
/locus_tag="AWJ20_4660"
/product="Pmu1p"
/transcript_id="XM_018881747.1"
/db_xref="GeneID:30036817"
CDS complement(177061..177663)
/gene="PMU1"
/locus_tag="AWJ20_4660"
/note="Putative phosphomutase; contains a region
homologous to the active site of phosphomutases;
overexpression suppresses the histidine auxotrophy of an
ade3 ade16 ade17 triple mutant and the temperature
sensitivity of a tps2 mutant; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0016853 - isomerase activity [Evidence
IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0008150 - biological_process
[Evidence ND]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]"
/codon_start=1
/product="Pmu1p"
/protein_id="XP_018736194.1"
/db_xref="GeneID:30036817"
/translation="MAQDYWSLLNGDGKIVWGPDAELTPLGIQQAKDNHTAWKEQISK
KIRLPQAYYSSPFTRSLDTMLYTWKGISLDFDNALRPLVMEDLREDIGVHTCDKRRTR
SFIEGRFGSIVDIEPQLTEEDELWSTQHRETHSEHDARSRDFLNYVFDLDWKSETPVD
YVSVTSHSGTTNSLLSVIGHRRFQLGTGGMIPVIVRAQKL"
gene <181907..>182890
/gene="HSP30"
/locus_tag="AWJ20_4662"
/db_xref="GeneID:30036818"
mRNA <181907..>182890
/gene="HSP30"
/locus_tag="AWJ20_4662"
/product="Hsp30p"
/transcript_id="XM_018881748.1"
/db_xref="GeneID:30036818"
CDS 181907..182890
/gene="HSP30"
/locus_tag="AWJ20_4662"
/note="Negative regulator of the H(+)-ATPase Pma1p;
stress-responsive protein; hydrophobic plasma membrane
localized; induced by heat shock, ethanol treatment, weak
organic acid, glucose limitation, and entry into
stationary phase; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA,IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence IDA] [PMID 1535043]; GO_component:
GO:0005886 - plasma membrane [Evidence IDA] [PMID
16622836]; GO_component: GO:0005886 - plasma membrane
[Evidence ISS] [PMID 8319300]; GO_function: GO:0005216 -
ion channel activity [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEP] [PMID 10206703]; GO_process: GO:0071361 -
cellular response to ethanol [Evidence IEP] [PMID
10206703]; GO_process: GO:0034605 - cellular response to
heat [Evidence IEP] [PMID 10206703]; GO_process:
GO:0070301 - cellular response to hydrogen peroxide
[Evidence IEP] [PMID 10206703]; GO_process: GO:0071470 -
cellular response to osmotic stress [Evidence IEP] [PMID
10206703]; GO_process: GO:0034220 - ion transmembrane
transport [Evidence IEA]; GO_process: GO:0006811 - ion
transport [Evidence IEA]; GO_process: GO:0032780 -
negative regulation of ATPase activity [Evidence IMP]
[PMID 9250391]; GO_process: GO:0006950 - response to
stress [Evidence IEA]"
/codon_start=1
/product="Hsp30p"
/protein_id="XP_018736195.1"
/db_xref="GeneID:30036818"
/translation="MSILVPRGNEALKLNPPFGLDIHITTHGSDWYWTVFSLFALASL
GGLVFSRIGPQTGSKFFYYNYMFSTFVLSIAYYTMASDLGWTGIQAEFNHATVDDGGL
VPGIRQIFYTRYVGWFLAFPPLVANFAVLSALPISTSFFTVLTVEVFVVSLLIGSLIH
STYKWGYFVFAVVSILLTAYNLFFSFRRAATSNVSHITGAVKTVTFGVSAVAGLMFLY
PLSWGLSEGGNVIQPDSEAVFYGVIDICMFIAVPTFFIFTTSTIEAESLGLRNFNTPL
FHSDNLAAEKEARYSGDTAVSNFAPTQPAAGTEPVSVPEPVATNPAAANAV"
gene complement(<183038..>184132)
/gene="NTG2"
/locus_tag="AWJ20_4663"
/db_xref="GeneID:30036819"
mRNA complement(<183038..>184132)
/gene="NTG2"
/locus_tag="AWJ20_4663"
/product="bifunctional N-glycosylase/AP lyase NTG2"
/transcript_id="XM_018881749.1"
/db_xref="GeneID:30036819"
CDS complement(183038..184132)
/gene="NTG2"
/locus_tag="AWJ20_4663"
/inference="similar to AA sequence:KEGG_Orthology:K10773"
/note="DNA N-glycosylase and apurinic/apyrimidinic (AP)
lyase; involved in base excision repair, localizes to the
nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose
from the whole genome duplication; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 10471279];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
19029246]; GO_function: GO:0051539 - 4 iron, 4 sulfur
cluster binding [Evidence IEA,IEA]; GO_function:
GO:0003906 - DNA-(apurinic or apyrimidinic site) lyase
activity [Evidence IEA]; GO_function: GO:0003906 -
DNA-(apurinic or apyrimidinic site) lyase activity
[Evidence IDA] [PMID 14500818]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0016787
- hydrolase activity [Evidence IEA]; GO_function:
GO:0016798 - hydrolase activity, acting on glycosyl bonds
[Evidence IEA]; GO_function: GO:0051536 - iron-sulfur
cluster binding [Evidence IEA]; GO_function: GO:0016829 -
lyase activity [Evidence IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0000703
- oxidized pyrimidine nucleobase lesion DNA N-glycosylase
activity [Evidence IDA,ISS] [PMID 9020769]; GO_process:
GO:0000737 - DNA catabolic process, endonucleolytic
[Evidence IEA]; GO_process: GO:0006281 - DNA repair
[Evidence IEA,IEA]; GO_process: GO:0006284 - base-excision
repair [Evidence IEA]; GO_process: GO:0006284 -
base-excision repair [Evidence IDA] [PMID 14500818];
GO_process: GO:0006285 - base-excision repair, AP site
formation [Evidence IDA] [PMID 9826748]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]"
/codon_start=1
/product="bifunctional N-glycosylase/AP lyase NTG2"
/protein_id="XP_018736196.1"
/db_xref="GeneID:30036819"
/translation="MPPRLRSRGLPASTVERASKRVKKEVAVKKETELTDGQLIKSEN
DGSTEVSTKSTVVVKTASEIEDGTLVTSVKSESSEILDKKSFVPPTLNYDPFKIIDIS
KIKSVSDKEPKNWRDIYSHVQKMRANTMAPVDTMGCERLPEAISSTITPAVHRYQLLI
ALMLSAQTKDEVNAQAMATLRRELSPVGGLTIDGILATQESEIDRMIFKVGFHSRKAK
YIKLSTQILRDQYDNDIPKTIEDMVSLPGVGPKMAHLLMHRAWGVAEGIGVDVHVHRL
ANMWGWTGKNSKLLPTPERTREALESWLPKELWVDINPLLVGFGQTICIPRGRKCDLC
TLAPTGLCKNVDRSKFKKVKRPIPDIEDLI"
gene complement(<184427..>186664)
/gene="CCR4"
/locus_tag="AWJ20_4664"
/db_xref="GeneID:30036820"
mRNA complement(<184427..>186664)
/gene="CCR4"
/locus_tag="AWJ20_4664"
/product="CCR4-NOT core exoribonuclease subunit CCR4"
/transcript_id="XM_018881750.1"
/db_xref="GeneID:30036820"
CDS complement(184427..186664)
/gene="CCR4"
/locus_tag="AWJ20_4664"
/inference="similar to AA sequence:KEGG_Orthology:K12603"
/codon_start=1
/product="CCR4-NOT core exoribonuclease subunit CCR4"
/protein_id="XP_018736197.1"
/db_xref="GeneID:30036820"
/translation="MQQYQMTGGKDGGGGAGAGAGAVNGGAIGGAIGSALGLGNPSNS
GSANNGSAAASEWMSSTHWQQQMQMAQLSRQTANSPHSYARSAAVASRTASGQNPIPL
TVTELAVQLVQQNQNQTQAAKNAAAGGNAVVGRPAGGMLNPHQRKEQLEEERQRRINE
DINKQFWTAIDLSGQGLTNMTPKLFNYNFLQKLYLNHNKLTSLPASVTKLGQLKVLDL
SDNLLTDLPAEIGLMYTLRYLFLFDNKLQELPYALGTLFQLEVLGLDGNPLSDHTREI
MAKEGTRGVIIDLRERAPVTIESPPREWLLFEEDKNNSKPRKDERTPQQLQQSRDNKD
KSNSKDDKKDGSSSANESDSSSVAGTSASSVTTTTNTSANSAVSNGPPSQSDFSIMSY
NTLCDRMATPQLYAYTPSWALGWNYRKERLLEEIVSIGTDIICLQEVDKISYDDLWVP
KLSKLGYTALHWPKGRARTMPESEAKKVDGCAMFYKHEKIKLIERHNIDYNLLALRKD
DFKKTADIYNRVMNKDNIAMINVFEQVQTGRLMIVANTHLHWDPAFKDVKLVQVALLL
EELEKVVDKYTSNSTPEGAKYPKLTDGKNIPLVVCGDYNSTTDSAVYQLFAQGKIEGT
HEDLEGRVYGKITDEGTTHKLGLKSAYSEIGELPFTNYTPNFVEVIDYIWYSTNSLSV
GGLLGQIDPEYTKHFVAFPNPHHPSDHIPLAAQFNFKKVKDAPPKHTSTPNFGNTSYG
SSRKT"
gene <189102..>189683
/gene="TSA1"
/locus_tag="AWJ20_4665"
/db_xref="GeneID:30036821"
mRNA <189102..>189683
/gene="TSA1"
/locus_tag="AWJ20_4665"
/product="thioredoxin peroxidase TSA1"
/transcript_id="XM_018881751.1"
/db_xref="GeneID:30036821"
CDS 189102..189683
/gene="TSA1"
/locus_tag="AWJ20_4665"
/inference="similar to AA sequence:KEGG_Orthology:K03386"
/note="Thioredoxin peroxidase; acts as both a
ribosome-associated and free cytoplasmic antioxidant;
self-associates to form high-molecular weight chaperone
complex under oxidative stress; deletion causes mutator
phenotype; protein abundance increases and forms
cytoplasmic foci during DNA replication stress; chaperone
activity is essential for growth under zinc deficiency;
required for telomere length maintenance; TSA1 has a
paralog, TSA2, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 10681558]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 22842922]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 8344960];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
18271751]; GO_component: GO:0005844 - polysome [Evidence
IDA] [PMID 18271751]; GO_function: GO:0016209 -
antioxidant activity [Evidence IEA,IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA,IEA];
GO_function: GO:0004601 - peroxidase activity [Evidence
IEA]; GO_function: GO:0051920 - peroxiredoxin activity
[Evidence IEA,IEA]; GO_function: GO:0051920 -
peroxiredoxin activity [Evidence IDA] [PMID 17210445];
GO_function: GO:0043022 - ribosome binding [Evidence IDA]
[PMID 18271751]; GO_function: GO:0008379 - thioredoxin
peroxidase activity [Evidence IDA,IMP] [PMID 7961686];
GO_function: GO:0008379 - thioredoxin peroxidase activity
[Evidence IDA,IMP] [PMID 9799566]; GO_function: GO:0051082
- unfolded protein binding [Evidence IMP] [PMID 16251355];
GO_process: GO:0000077 - DNA damage checkpoint [Evidence
IGI] [PMID 19851444]; GO_process: GO:0042262 - DNA
protection [Evidence IMP] [PMID 19543365]; GO_process:
GO:0045454 - cell redox homeostasis [Evidence IDA,IMP]
[PMID 8344960]; GO_process: GO:0045454 - cell redox
homeostasis [Evidence IMP] [PMID 9799566]; GO_process:
GO:0034599 - cellular response to oxidative stress
[Evidence IGI] [PMID 15051715]; GO_process: GO:0034599 -
cellular response to oxidative stress [Evidence IMP] [PMID
18271751]; GO_process: GO:0034599 - cellular response to
oxidative stress [Evidence IDA,IMP] [PMID 8344960];
GO_process: GO:0061077 - chaperone-mediated protein
folding [Evidence IMP] [PMID 24022485]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]; GO_process: GO:0006457 - protein folding
[Evidence IMP] [PMID 16251355]; GO_process: GO:0001302 -
replicative cell aging [Evidence IMP] [PMID 20729566];
GO_process: GO:0033194 - response to hydroperoxide
[Evidence IMP] [PMID 15210711]"
/codon_start=1
/product="thioredoxin peroxidase TSA1"
/protein_id="XP_018736198.1"
/db_xref="GeneID:30036821"
/translation="MVATVRHPAPAFTKKAVVNGVFEDVSLEQYKGKWVILAFIPLAF
TFVCPTEIIAYSDAVKEFADRDAVVLFASTDSEFSLLAWDNVSKKDGGLGNVKIPLVA
DTNHSLSRDYGVLVEEEGIALRGIFLIDPNQVVRQITINDTPVGRSVPETLRLLDAFQ
FTEKHGEVCPANWQKGDATIDTSKASEYFQKQA"
gene complement(<189995..>193624)
/gene="VAC7"
/locus_tag="AWJ20_4666"
/db_xref="GeneID:30036822"
mRNA complement(<189995..>193624)
/gene="VAC7"
/locus_tag="AWJ20_4666"
/product="Vac7p"
/transcript_id="XM_018881752.1"
/db_xref="GeneID:30036822"
CDS complement(189995..193624)
/gene="VAC7"
/locus_tag="AWJ20_4666"
/note="Integral vacuolar membrane protein; involved in
vacuole inheritance and morphology; activates Fab1p kinase
activity under basal conditions and also after
hyperosmotic shock; GO_component: GO:0070772 - PAS complex
[Evidence IDA,IPI] [PMID 19037259]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IDA] [PMID 9372916]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IDA] [PMID 9372916]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005774 -
vacuolar membrane [Evidence IEA]; GO_component: GO:0005774
- vacuolar membrane [Evidence IDA] [PMID 19037259];
GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0033674 - positive regulation of
kinase activity [Evidence IGI] [PMID 11950935];
GO_process: GO:0010513 - positive regulation of
phosphatidylinositol biosynthetic process [Evidence IGI]
[PMID 11950935]"
/codon_start=1
/product="Vac7p"
/protein_id="XP_018736199.1"
/db_xref="GeneID:30036822"
/translation="MSSSRQPPARAAGKGGTPSGTRPVAPSPRRVSITSSEDLSYSTS
EELDESDDYFSYQKGKSIASGGAGAAGGSAGSGISSRASTASYSGVNPSGRSPYQFPP
PSKQLLRQNHRPNYGSNYGSGYGSGYGYSYHNDREGEGDGENEDYDDGDVEEYDDEDD
RAGHGTIKANMLPSHSIGADTSSSSDNNSSSARFKGKIKHSSSNSSSTIKRDKSSNTL
KSGSSSQSSPSKRYSRSSRHKKQLSNGTIRASDSGTQQDAKNDDNDTTADDNDGDTTI
SNLRISKDKQKNVDDKDGETTVHDKNTTGTAASSGTGASGKTKGGSANPSVNTSSLTS
GTTNTASAASTAPSTASTATTTTTTDSSILASSGDLPSSKSGKEQTGSASTENNSAVA
SRTGDNKIGSGPATATILPSSTSAPNIPVAMAMSGASVTAPPTSTITGAGSGLGINSG
ITPSSVNLPAKASGSAASIKKPNTMTVEAETVEAVPTIAVAPTIAGSGSLKVKKSTDN
VMKPINRTKKKKSSRLGQGSKAEIFAAKIASAVDEVHSSDSDETFVYESNPPDRSDSQ
RQHHQPLSSSQQSAVPSRPRFQSRNPSTSSLPFTGSQQQLQLQQLQQQIQAQQQQIHQ
NYREQTQHQLLQQQYQEQQQQQQHSQTLQQQLPQQTQQSQQQTQPTQQTQQLTGQTQP
GTSQPTTTGAPSLQQTALGLAISSVPGSSSTQGSSVEPSGNVAQMNRWVVSKHKRTSG
DTPMSSSSSITNVPVLESNSGGATGTTGGSTSGGTTAGGSGSVGAGTFLIARDRDTRS
NGSSKRSRGAGGSSRAESPRPEDFESPKFYTHSGPSHSPLPRKLTANRHYDSTASYKA
KDATLRRWRGGGSYMAEEDDDYDDQPEQDDMDDDIEYLNVSETTPLRSSGHSIRRSRK
AGSLVAYSPHNYQHGKSWSQFYYFRIMFWAAVFGVCVLATGFILGFLLATSKPLQETR
VSQIFDVLVSDEVLAFEVVIEAYNPGYLGIEVQNVNLDLFAKSKHVTDLQRETTTSTK
PDDDESHTMLLGNVQKFDVPLIFEGGIFSKRLQKAVGDVKLVNPGRNTTGDDLVKESL
AVLPPSPSMWQLLQQALKEQNGELDEGQKKWSRVNVHSFDLILRGSLKYKLLFGRERS
VSIAKVSPPPSVPPAAGAPPQTPLLLSLRSSRHVHGPCLLRSRSNQGLGRSPSRRRQS
PSRQN"
gene <195708..>198686
/gene="SWE1"
/locus_tag="AWJ20_4667"
/db_xref="GeneID:30036823"
mRNA <195708..>198686
/gene="SWE1"
/locus_tag="AWJ20_4667"
/product="tyrosine protein kinase SWE1"
/transcript_id="XM_018881753.1"
/db_xref="GeneID:30036823"
CDS 195708..198686
/gene="SWE1"
/locus_tag="AWJ20_4667"
/inference="similar to AA sequence:KEGG_Orthology:K03114"
/codon_start=1
/product="tyrosine protein kinase SWE1"
/protein_id="XP_018736200.1"
/db_xref="GeneID:30036823"
/translation="MNSNSVTPAPNMSPLRHHHGHGHSHGHSTSNSSQNQSHSMASNH
DLAAPVPFRKTLRRSTRLLNLEQAAGRRDSDGDMIDVDDDVSIDIGGLSGLGLSGLAN
DGDSDMNTTNANDNSGSNGQLGINGLDIGSSGHHSMVSGRTVGPASSSSDSGSLVASS
SSFSLKRPHPSSSDMRSPSSSFPKPRFLSRNAFDSESNHSHGHKSIGPTISGSASTPT
PSSSNGGSSNSSQFLPSLWSSSLKRRDSFDQLFAPSSPFAINGQSTPSHSNSHSGNST
TTTSNNINPGNNANTSFNFQTNNPYTTSPSPSPIPASKSHHIHIQHQASASSTASQHH
NPLEPTTHHSPHSPHSPHHSPHHSPHRSPHHLPLPRPPLPRHAPPQMHRPKPKFNPSV
TAEFSDYHTHTPENYRSVKPLQTAFMSTGLLSKRNRMNNSNSQADGLRMPPDTPCKRP
SNMSLPLINHHNTSMFGSAPTAYSPHVSNIGGGFGTTSSGRRRSNLGLHDSLLRFSVD
FGNTSTQVAEADSPMHDHDDMIDHQPPETPTKETSGHDMSSFLSSHLAKYSGDTSVSS
TVSTSLTAGDTSGTIETANTTVDLSGSHPNLTFSAYSLADRGNHGNSFSSYPTPLPMN
IPNRAQSQAGSTGSELGSGGASSGSDKVISTTPQSRNSRWTDGSSSEDTLSSPRTPDV
IMHSDPSTIIGGSTNNSTSTVTTTSGHTSIPMIRRTTSTTSLIQEPPRTPAKTPKTPI
DPLMNSSNFISLRKSSVDVDDPVLNERFDRVSLIGRGEFSIVYSLSIRGDDSPTDPTS
PAIPHTLAHDNRYAVKRTRFPFMGPKARSRRLEEVEILRALTAPSRHSHPDDEHDPAD
DGRDYVLMLLDAWEANGYLYIMTEFCENGSLDTFLSERGNISRLDEWRVWKILLEIAL
VSSLSTHTCLRRLGLRPRPWLLLSLRSSRCVDGPGLLRSRSSQGLGRSPSRRRQTTGK
SSNSVGIALYPRSWVFAFGY"
gene <198718..>199479
/gene="SWE1"
/locus_tag="AWJ20_4668"
/db_xref="GeneID:30036824"
mRNA <198718..>199479
/gene="SWE1"
/locus_tag="AWJ20_4668"
/product="tyrosine protein kinase SWE1"
/transcript_id="XM_018881754.1"
/db_xref="GeneID:30036824"
CDS 198718..199479
/gene="SWE1"
/locus_tag="AWJ20_4668"
/inference="similar to AA sequence:KEGG_Orthology:K03114"
/codon_start=1
/product="tyrosine protein kinase SWE1"
/protein_id="XP_018736201.1"
/db_xref="GeneID:30036824"
/translation="MLKIGDFGMATAYPAVKGIEREGDREYIAPEVLSSQQYDKPADV
FSLGIMMLEIAANIVLPDNGIHWQKLRSGDLTDAGRLSSGDLRRANEYGDDEDDECDD
DNCDDDSEHVRLSLDNDSRTSLEDETTSPRMPPSQFSDIHMSSHNSGASNRKVPRRLS
AIPPWAPKFMVDKSGALDVMVKWLLNPDPHSRPTTHEILISEECQWVEAHRRAGAVIY
EGDYGPEPDPVGSAEDHARQMLRPDHTQDNWRRGV"
gene <202093..>205500
/gene="SYP1"
/locus_tag="AWJ20_4669"
/db_xref="GeneID:30036825"
mRNA <202093..>205500
/gene="SYP1"
/locus_tag="AWJ20_4669"
/product="Syp1p"
/transcript_id="XM_018881755.1"
/db_xref="GeneID:30036825"
CDS 202093..205500
/gene="SYP1"
/locus_tag="AWJ20_4669"
/note="Negative regulator of WASP-Arp23 complex; involved
in endocytic site formation; directly inhibits Las17p
stimulation of Arp23 complex-mediated actin assembly in
vitro; may regulate assembly and disassembly of the septin
ring; colocalizes and interacts with septin subunits;
potential role in actin cytoskeletal organization;
GO_component: GO:0005935 - cellular bud neck [Evidence
IEA]; GO_component: GO:0005935 - cellular bud neck
[Evidence IDA] [PMID 11014808]; GO_component: GO:0005934 -
cellular bud tip [Evidence IDA] [PMID 11014808];
GO_component: GO:0001400 - mating projection base
[Evidence IDA] [PMID 11014808]; GO_component: GO:0043332 -
mating projection tip [Evidence IDA] [PMID 19053807];
GO_component: GO:0005628 - prospore membrane [Evidence
IDA] [PMID 24390141]; GO_function: GO:0004857 - enzyme
inhibitor activity [Evidence IDA] [PMID 19962315];
GO_process: GO:0000147 - actin cortical patch assembly
[Evidence IMP] [PMID 19776351]; GO_process: GO:0007049 -
cell cycle [Evidence IEA]; GO_process: GO:0006897 -
endocytosis [Evidence IEA]; GO_process: GO:0043086 -
negative regulation of catalytic activity [Evidence IDA]
[PMID 19962315]; GO_process: GO:0032185 - septin
cytoskeleton organization [Evidence IGI,IMP,IPI] [PMID
18791237]"
/codon_start=1
/product="Syp1p"
/protein_id="XP_018736202.1"
/db_xref="GeneID:30036825"
/translation="MATGRSEYSNAILPNLAPDRAVDVVRDRLNNAKLLNDEIADWLK
ERARIEESYASELDKLSRRGPLAHEQALGTFTSVWNELTRSARETSKASADYAKKTRT
DIEAPIRSFSQKSPQWSDVRDLHDGLSGVAKNLVDGEDNLTKLRTKKSSSSKIEAAQH
AVNESRNQWENQGPYVLGQFEIADEARFALLRDSLTRLETLEADKGQAIIKGSEKVLN
SILSFDPIEEVRSFAAQIVAGNIGSVSSSGTAGVGGGSSGGSGLARHSQSRDSGDVDS
QHFNRGGLVGNIHRPSRNTLDDSSSINSGNTPLGGSGGASKLRSKVGSIFRGGRKKKG
EKEAAEIGAGLAAGAGAAAVAGRSNLGAGSRSASTASARAPGRRGTDSSDLYTPVDPS
VAAARQQQQQQQQQQQQQQQQQQQQQQQQPQAQQQSSLQQPLQPQQAQDHLSGSKPQP
PPSRKVNGQAQREFREEDEYEDEENQPLQNLVSGQQQPFRVNIRSDVITEEQEDANTA
LSNVASTLRSKPTISGRGSRGRRDIQSRLFTEIEASDLESSNAAAQDQSGPSYVGQQF
FNQLQSFPGQGQGSTQVPSESQNGFSAPQHAISGTTAAGAAIGAAVGSLPVLNEVEPA
IKREFEPQTQQGDSFNFNQPQPQQPQQQYQQSFADTQLQQGQQFNGHQFPDTQLQQGQ
QGEQYNGQQFPQEYNQQQPQYNQQQQISTSQAQIQQPVYSEQTAGSFGQQTAPQLPPI
NTDSSFHGDIGGDRSLGSATEPIVSPSGSAIADQRAPAGNVFAPVPTSVGQSTSLSSP
GAGASPAGDLQPPMSAFRANRTNSDVQSLRSLQSSTTGGANIAALKHPDLPTGPGLVA
SIVEVITVDMKDGVPTKTAVGGEVSVAYNETDDAATPGTIYVKVKTKQTKLDRLVANN
QIVVASSEPDTFKLDLSSPATASSLLGVRQGAKALMYTLSGDQHGYNPLVFTPIWRIE
ETQSSLMLNYELADTFHGSELEIHDLVISVPIEGGHAHSAQNKPVASFNKDRQRITWR
FPEPVVLKKGQKEKLLCRFATDGYAHEASSGLEVRFRLTDSASSQLSQLGLEYTTKTE
EDPFSDEATNTSAVSLSSHLNWVPVSVSRSLVTGKYSVHSELNVSKR"
gene complement(<205760..>206533)
/gene="MAK16"
/locus_tag="AWJ20_4670"
/db_xref="GeneID:30036827"
mRNA complement(<205760..>206533)
/gene="MAK16"
/locus_tag="AWJ20_4670"
/product="ribosome biosynthesis protein MAK16"
/transcript_id="XM_018881757.1"
/db_xref="GeneID:30036827"
CDS complement(205760..206533)
/gene="MAK16"
/locus_tag="AWJ20_4670"
/inference="similar to AA sequence:KEGG_Orthology:K14831"
/codon_start=1
/product="ribosome biosynthesis protein MAK16"
/protein_id="XP_018736203.1"
/db_xref="GeneID:30036827"
/translation="MKTAERAHTPAKLWERVKLSKQYSKALEQIDQQLIYWPNFLIHK
CKQRLTRLTQVAITERRLALKENERQYVGKAPKVKRREQTRERKALVAAKLEKAIEKE
LLDRLKTGAYGENPLNVDEKIWKKVLNGVEKEEGVEQEEEFDEDEELEDESDVGEVEF
VEGDDDEDDIVELEDLEKWLGGSDDDGEQRFDSSEDESSSDEESEDDEDEQETKTRKR
KPTPAKKPKSDKKKPRRGPRVEIEYEQERESNLQTNVNW"
gene <208769..>211453
/locus_tag="AWJ20_4671"
/db_xref="GeneID:30036828"
mRNA <208769..>211453
/locus_tag="AWJ20_4671"
/product="hypothetical protein"
/transcript_id="XM_018881758.1"
/db_xref="GeneID:30036828"
CDS 208769..211453
/locus_tag="AWJ20_4671"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736204.1"
/db_xref="GeneID:30036828"
/translation="MFAPRIFEESDQSISVADRSMFESRNADDMTNNNASPASGLRRS
HTVSGSPSRTTYGSRWGDNSRKKVVAGTRYFSGDKSVELNAQSSAKRSGISGGANNET
IEATRREILQSLERSPLKLYPDQHTDSQFPTEETRSLSKNRKLATSENKENGGQFVVP
AAAAPAEDDDLTNFSKYDIRNISYQSVSRASPKRPQDSNKSIGNIGLRSRSKQPLPTD
DSQRHHFADNEDRREPGELFERRTFSEEHHNAEDEREYARSQQSVREADNILESTDRR
QVRNRSREEVGDPTPEIASYRDHEPVHSNFLREEVLQQENDFSRKPTREPIRQTIREP
IHEPIREPVREQLHDFGRELTREPIRDPIREQENASGRELTREPVHKPVREPVRESIR
ESVPESVRDLTRNRRFEVDGEVSYRVDTQVRSDSQARGYRQELLHESAGTSVREHTEE
SSEREQLTREPVRNLGQDQQRENPRMYEQDAPPRQQTRNDYQSGTSYRARDRRQSMYT
DRANQTVNHDSTYHARSAQTGLSPNATGTSRKEPNMGNMTISGIPSPKKIKQPVLARS
KSQSRIRGPPSPTKGPYDPETNPPALKKRSVSDDRKKRQSIAILPPERERERELDRDR
ERERANRRQSVVPRMVGTTTTAGSTTNYHPTTATTTATSGTTTNRQMAFGRTPGRLLA
PKSTAELRANINAGINNNAGGSGTKTASGVSGTTNNHNSTQAQTKTNSTSQTQQKNKD
EISILEVDSTKPSEQSSQQLRIEMERKLAQHRTEIVSLKARLSGKDSEIKSLKASVEQ
SELTIESEARQREEMVARVAKELHVQYNKKHEAKLAQFKQSVKIKEETLTSEIHMLRA
EVENLKTRIAVERDEKAELVNAFEAYQVELERMGIRLG"
gene <212643..>217349
/gene="BEM3"
/locus_tag="AWJ20_4672"
/db_xref="GeneID:30036829"
mRNA <212643..>217349
/gene="BEM3"
/locus_tag="AWJ20_4672"
/product="Bem3p"
/transcript_id="XM_018881759.1"
/db_xref="GeneID:30036829"
CDS 212643..217349
/gene="BEM3"
/locus_tag="AWJ20_4672"
/note="Rho GTPase activating protein (RhoGAP); involved in
control of the cytoskeleton organization; targets the
essential Rho-GTPase Cdc42p, which controls establishment
and maintenance of cell polarity, including bud-site
assembly; GO_component: GO:0005938 - cell cortex [Evidence
IDA] [PMID 17914457]; GO_component: GO:0005934 - cellular
bud tip [Evidence IDA] [PMID 17914457]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 17914457];
GO_component: GO:0000131 - incipient cellular bud site
[Evidence IDA] [PMID 17914457]; GO_component: GO:0005622 -
intracellular [Evidence IEA]; GO_component: GO:0043332 -
mating projection tip [Evidence IDA] [PMID 17914457];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 19053807]; GO_function: GO:0005096 - GTPase
activator activity [Evidence IEA]; GO_function: GO:0005100
- Rho GTPase activator activity [Evidence IDA] [PMID
12455995]; GO_function: GO:0005100 - Rho GTPase activator
activity [Evidence IGI,IPI] [PMID 7498791]; GO_function:
GO:0005100 - Rho GTPase activator activity [Evidence IDA]
[PMID 8227021]; GO_function: GO:0005100 - Rho GTPase
activator activity [Evidence IDA] [PMID 8300560];
GO_function: GO:0035091 - phosphatidylinositol binding
[Evidence IEA]; GO_function: GO:0032266 -
phosphatidylinositol-3-phosphate binding [Evidence IDA]
[PMID 11557775]; GO_process: GO:0030010 - establishment of
cell polarity [Evidence IMP] [PMID 8013906]; GO_process:
GO:0035024 - negative regulation of Rho protein signal
transduction [Evidence IGI,IMP,IPI] [PMID 17914457];
GO_process: GO:0043547 - positive regulation of GTPase
activity [Evidence IEA]; GO_process: GO:0031106 - septin
ring organization [Evidence IGI] [PMID 14517318];
GO_process: GO:0007165 - signal transduction [Evidence
IEA]"
/codon_start=1
/product="Bem3p"
/protein_id="XP_018736205.1"
/db_xref="GeneID:30036829"
/translation="MASRISSHLPSNPLGEPYNSPSSISVADSASGDGHSEITHGSNG
LGAGAAGGPYGGSSGPVASSVTTDAASGADVSIVPGESIEGRGLTMSRSSSNYSLNGR
DDFNGDDDEDDDYENQQRNQNEHQLKNQNQTEIHNQNESQRENESQGYNNYNNENDDP
STRIHRGRSDVKSEHGPESAPGYLPPNEPTTNTHEPLDPKIPSPPAHASEAGTTGSGA
TPQPPEADPPSTKPQSKPRSESRSSRSRSRPTKGVSSDASWVKESGSGEAKDASKSVH
EPHSQAVINQLVREKLELDLLVNEKVLQLSELQLQNSKLWKLVKRQHLTILDLQKDLD
EAVDQNERLRSQTQSPVKNRSTAASASTSTIHSEEGSEDSSAGVGARSSNNDIDSVGS
NSKAIINNMKTLRASLLPAESSSVIKTVAEPTTTVVGTNATPRTSVDSTSQSRARTET
ESTATPRTSAREAASSVDVSSPTVSESASNSSVSLNLDSTASVIYSPMQKAHKVSVER
TRGRDNERALDGALSPFNDISTPRDYHLQQPLSQTQSDHQHHHQHHHQQQQQQQHQQL
QRELFGSPTREEHQQQPNHLHVPALGTPLRDTLGTPLRDRLTSPSRNATYEPLLNSGI
NLPQVLQPGPPLESLGPPLFQSTTSTSSASETFAPSVSITDDVSGRASALGLGASSGN
VSDEPADNPANDSTSTSDREYLVLPARSKSRPPIGERLARANTPPVAGDSLSVETTNH
HLAEPTTPTPASSSGTSGSANSSAKRRPPPLLFNNNDKYGLNDTTAVRALSPEAPLTS
QLPRSAPATSNSTSSTAGAYSSTAPSTTPGTASSTSSASSAGKSPFGALDISLITIEV
KNTLFGKPESKNASKDDPVAILNCIDRETGAVLFKIVKDYTQLSNLDQSLRKFSSQYS
IPPLPERNLFLSQAPIKVDLRRNQLNSYFGAIMSVPELPQEAVILLIKFLTQNVLPKT
AIASANPYGSLTAGKCGFLTKRGRLTSWKSRWFVLNGSSLDYYERPESDAPIGTISVY
KAKIGRPRKDADESDEGENEYRHAFLLMEWKKNGYVRHVFCAETDEDRDLWINALLEL
VEVVPGTSTSNNNNNSNGSAGESLASSVSTSTLGTAVNAASNPNAYKPMSPRSVKSDE
GDYSNMSSVTVLSPPPSSSIGMNYDGHHGSNSSVSSNGGTTNTAAGGSFPKTPISGPL
SFQKILDPGSWNSPNRNNNNNSDINHGHYVGGSGAGSGSHHVSHGSHGSNNVHSFGSE
SDETKRKERKKYLSIFKKGGHSPMSSNTSAHSVNAAFPNGFPSMNSINQLQSPPPPPP
PPPPPPPPPLNLSGTRSPGSATFGDDNYYNDSIDTSMDHHGAHRQYRGTVFATPLPQA
IAISSKNVNGCIVPSILYRCIELLERKGAIYEEGLFRLSGSDSAIRGLKERFNSEYDV
DLIAAGADIHAVSGLLKRYLREISEPVIPPLTELQFRTEMESDDAYVRSTAAKNLVSV
LPRENYDLLCVLYSFLRKIVDNMNLNKMNLNNLSIVFSPTLHITTNVFSTLFENYSAI
FKGFQSDITDPAANVL"
gene complement(<217620..>218831)
/gene="LIP5"
/locus_tag="AWJ20_4673"
/db_xref="GeneID:30036830"
mRNA complement(<217620..>218831)
/gene="LIP5"
/locus_tag="AWJ20_4673"
/product="putative lipoate synthase"
/transcript_id="XM_018881760.1"
/db_xref="GeneID:30036830"
CDS complement(217620..218831)
/gene="LIP5"
/locus_tag="AWJ20_4673"
/inference="similar to AA sequence:KEGG_Orthology:K03644"
/note="Protein involved in biosynthesis of the coenzyme
lipoic acid; has similarity to E. coli lipoic acid
synthase; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 11914276];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0051539 - 4 iron, 4 sulfur cluster binding [Evidence
IEA,IEA,IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0051536 - iron-sulfur
cluster binding [Evidence IEA,IEA]; GO_function:
GO:0016992 - lipoate synthase activity [Evidence
IEA,IEA,IEA]; GO_function: GO:0016992 - lipoate synthase
activity [Evidence ISA] [PMID 8349643]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0016783 - sulfurtransferase activity
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0009107 - lipoate
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0009249 - protein lipoylation [Evidence IEA];
GO_process: GO:0009249 - protein lipoylation [Evidence
IMP] [PMID 19570983]"
/codon_start=1
/product="putative lipoate synthase"
/protein_id="XP_018736206.1"
/db_xref="GeneID:30036830"
/translation="MLKVTASRGSICGPLRAISSFHTSAYALLESTDSVKSGATSTAP
TRSSRSTRFTDKLNKGPSFEDFLSKKAASDPTLLDPVVAARKSEDARLPKWLKTEIPK
GKNFHKLKDDLRGLKLHTVCEEARCPNIGECWGGSDKSKATATIMLMGDTCTRGCRFC
SVKTNRNPSPLDPNEPENTAEAIAKWGLGYVVLTTVDRDDLIDGGAYHFADTVKRIKK
KASHILVECLTGDFRGDLNMVKIMATSGLDVFAHNVETVEDLTPYVRDRRATFQQSLA
VLAHAKQSNPDVLTKTSVMLGFGETDAQVEDTLQQLRKADVDVVTFGQYMRPTKRHMK
VAEYVTPEKFQHWHDRAMELGFKYCASGPLVRSSYKAGEAFIENMLKNKQKLAASASA
TTSASSATATA"
gene <218227..>218514
/locus_tag="AWJ20_4674"
/db_xref="GeneID:30036831"
mRNA <218227..>218514
/locus_tag="AWJ20_4674"
/product="hypothetical protein"
/transcript_id="XM_018881761.1"
/db_xref="GeneID:30036831"
CDS 218227..218514
/locus_tag="AWJ20_4674"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736207.1"
/db_xref="GeneID:30036831"
/translation="MRSSLSTVVNTTYPRPHLAIASAVFSGSLGSNGEGLRLVLTEQN
LHPLVQVSPINMIVAVALDLSEPPQHSPIFGHLASSQTVCSFNPRRSSFNL"
gene <222116..>223063
/gene="CAR1"
/locus_tag="AWJ20_4675"
/db_xref="GeneID:30036832"
mRNA <222116..>223063
/gene="CAR1"
/locus_tag="AWJ20_4675"
/product="arginase"
/transcript_id="XM_018881762.1"
/db_xref="GeneID:30036832"
CDS 222116..223063
/gene="CAR1"
/locus_tag="AWJ20_4675"
/inference="similar to AA sequence:KEGG_Orthology:K01476"
/note="Arginase, catabolizes arginine to ornithine and
urea; expression responds to both induction by arginine
and nitrogen catabolite repression; disruption decreases
production of carcinogen ethyl carbamate during wine
fermentation and also enhances freeze tolerance;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005829 - cytosol [Evidence
IDA] [PMID 205532]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 19053807];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0004053 - arginase activity
[Evidence IEA,IEA]; GO_function: GO:0004053 - arginase
activity [Evidence IMP] [PMID 14582193]; GO_function:
GO:0004053 - arginase activity [Evidence IDA] [PMID
2404017]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0016813 - hydrolase
activity, acting on carbon-nitrogen (but not peptide)
bonds, in linear amidines [Evidence IEA]; GO_function:
GO:0030145 - manganese ion binding [Evidence IDA] [PMID
1939179]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA,IEA]; GO_function: GO:0090369 - ornithine
carbamoyltransferase inhibitor activity [Evidence IDA,IMP]
[PMID 12679340]; GO_function: GO:0008270 - zinc ion
binding [Evidence IDA] [PMID 1939179]; GO_process:
GO:0019547 - arginine catabolic process to ornithine
[Evidence IMP] [PMID 14582193]; GO_process: GO:0019547 -
arginine catabolic process to ornithine [Evidence IDA]
[PMID 2404017]; GO_process: GO:0006525 - arginine
metabolic process [Evidence IEA,IEA]; GO_process:
GO:0090368 - regulation of ornithine metabolic process
[Evidence IDA,IMP] [PMID 12679340]; GO_process: GO:0000050
- urea cycle [Evidence IEA]"
/codon_start=1
/product="arginase"
/protein_id="XP_018736208.1"
/db_xref="GeneID:30036832"
/translation="MTISQKSRSLTVISAPFSGGQGKPGVDDGPQHLLDMGLVEQIGR
LDWTPKFAGHLQFPAVKNDTNIGHVKNPRYVSQSTKNIYEAVKKASTSSDFTLTIGGD
HSIAIGTVAGVQAAHPDAIVLWIDAHADLNTPAASSSGNLHGCPVAFLLGADEPLANA
ASKEEADLFGWVPHCLDDSQLGYIGLRDVDAFEKQYIREHKIAAFSMHHVDKYGIASV
VEQAINKLDPEHKRPIHLSFDVDAIDPVYAPSTGTPVRGGLTWREACYLCEAVAETGR
LVAMDLVECNPHLEDAKAARETIAMGLSLVRCALGETLL"
gene complement(<225880..>228504)
/gene="HOS3"
/locus_tag="AWJ20_4676"
/db_xref="GeneID:30036833"
mRNA complement(<225880..>228504)
/gene="HOS3"
/locus_tag="AWJ20_4676"
/product="Hos3p"
/transcript_id="XM_018881763.1"
/db_xref="GeneID:30036833"
CDS complement(225880..228504)
/gene="HOS3"
/locus_tag="AWJ20_4676"
/inference="similar to AA sequence:KEGG_Orthology:K11484"
/note="Trichostatin A-insensitive homodimeric histone
deacetylase (HDAC); specificity in vitro for histones H3,
H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and
Hos2p; deletion results in increased histone acetylation
at rDNA repeats; GO_component: GO:0005935 - cellular bud
neck [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11914276]; GO_component: GO:0005628 - prospore membrane
[Evidence IDA] [PMID 24390141]; GO_function: GO:0032041 -
NAD-dependent histone deacetylase activity (H3-K14
specific) [Evidence IEA]; GO_function: GO:0097372 -
NAD-dependent histone deacetylase activity (H3-K18
specific) [Evidence IEA]; GO_function: GO:0046969 -
NAD-dependent histone deacetylase activity (H3-K9
specific) [Evidence IEA]; GO_function: GO:0046970 -
NAD-dependent histone deacetylase activity (H4-K16
specific) [Evidence IEA]; GO_function: GO:0004407 -
histone deacetylase activity [Evidence IDA] [PMID
10535926]; GO_function: GO:0031078 - histone deacetylase
activity (H3-K14 specific) [Evidence IEA]; GO_function:
GO:0032129 - histone deacetylase activity (H3-K9 specific)
[Evidence IEA]; GO_function: GO:0034739 - histone
deacetylase activity (H4-K16 specific) [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_process: GO:0016568 - chromatin modification
[Evidence IEA]; GO_process: GO:0070932 - histone H3
deacetylation [Evidence IEA]; GO_process: GO:0070933 -
histone H4 deacetylation [Evidence IEA]; GO_process:
GO:0016575 - histone deacetylation [Evidence IDA] [PMID
10535926]; GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 17706600]; GO_process: GO:0000083 - regulation
of transcription involved in G1/S transition of mitotic
cell cycle [Evidence IGI,IPI] [PMID 19823668]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Hos3p"
/protein_id="XP_018736209.1"
/db_xref="GeneID:30036833"
/translation="MAENTGSQSGHGYDPNGGWDVNSGDNSKPRPSFSEYLNFDDDGD
TTEEDMELFSDKYVGRLGNGDLGDDELNAALEAMNLDGSEHGLHTHQNQFARPGAGVY
GSQEGSLENSLVLEQSSGNEVNMPVMGSFQPRQEQDTLGSFNDNFGDSQDRERVNLRP
VILPPPSSELYNVRDPFSRDAFTPEAASRESTFETSVDSTVDSTLETSVDTSVADVKS
DPLPSTKLAESTVRETINTEPQIERRTEEKLKDPGAETGESGSGELPTTPKNKLIDAP
IALPDDTDLSEFNSAFAQLKIGQDAISSSTEKKTLILLSPLSYKHVFSRNWVKKSYLD
SIVERPERLMAASLGIGTAIAKSPTKYTVQVSDRRSDLRQASHVSIVHGSKWAKRLYQ
LCNDSVEKLKNGDVEVPGDWHEGDIYLTGETIDALEGVVAVSETAVDKIYAEGESKVF
VSIRPPGHHSHPCVPSGFCLINNVHIAIQYARAKYGITHAVILDFDLHHGDGSQDICW
KLSGLDDDEEENEEADSENEQHQENQSNSNEHSEPTVKTEQTPSSTTPAESLQTSTPT
SSSQDNESPSKSPRKKGKKTSHPPPPPPPPPLSIGYFSLHDINSFPTEFGYATAENIK
NASVCVMAHGMCIWNVHLEPYRDEAHFNEIYSNQYSALFEKARQFLQTGKTTSAHNKQ
PFKPLIVLSAGFDSSEYELLSMQRHQVSVPTSFFNRFTNDSVKLANEFTDGKIISMLE
GGYSDAALSTGIFSHLTGLAEEQWDSNWCNPTVAKTLAKGSKLKWKRPATTSTSSTNS
HHTTSPTASEPHWLNPGIELGRSLWPSQIKAQVLAATKPSASTTGRRNRKWDDDSTTS
VLATPLRVLRDKTRKSVH"
gene <229381..>230076
/locus_tag="AWJ20_4677"
/db_xref="GeneID:30036834"
mRNA <229381..>230076
/locus_tag="AWJ20_4677"
/product="hypothetical protein"
/transcript_id="XM_018881764.1"
/db_xref="GeneID:30036834"
CDS 229381..230076
/locus_tag="AWJ20_4677"
/inference="similar to AA sequence:KEGG_Orthology:K18159"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736210.1"
/db_xref="GeneID:30036834"
/translation="MRTGLVASPVPTELGLVNFREPALGLANLLVRCDKDLGGFSTAD
LSVVESDSTSNHAFGRFKGDLSLDLPADRPDIIRLGYAMFRTKDIEPKLFGLASSEYW
NWEPYNQMVLRVRGDRRKYFINVQAESAYPTDLYQHRLFLKTPGQWEEVTVPLGNFIL
TNGGIIQQQQAMELSQVKSVGIGLIDRQYGPYQLDIDWIKVVTGDGPLKPAKPTKPSS
ADLTPGKRLSIDD"
gene complement(<230292..>232610)
/gene="MNL1"
/locus_tag="AWJ20_4678"
/db_xref="GeneID:30036835"
mRNA complement(<230292..>232610)
/gene="MNL1"
/locus_tag="AWJ20_4678"
/product="alpha-1,2-mannosidase MNL1"
/transcript_id="XM_018881765.1"
/db_xref="GeneID:30036835"
CDS complement(230292..232610)
/gene="MNL1"
/locus_tag="AWJ20_4678"
/inference="similar to AA sequence:KEGG_Orthology:K10084"
/note="Alpha-1,2-specific exomannosidase of the
endoplasmic reticulum; in complex with Pdi1p, generates a
Man7GlcNac2 oligosaccharide signal on glycoproteins
destined for ubiquitin-proteasome degradation;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005788 - endoplasmic reticulum
lumen [Evidence IEA]; GO_component: GO:0005788 -
endoplasmic reticulum lumen [Evidence IDA] [PMID
19279007]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_function: GO:0004559 - alpha-mannosidase activity
[Evidence IEA]; GO_function: GO:0005509 - calcium ion
binding [Evidence IEA]; GO_function: GO:0030246 -
carbohydrate binding [Evidence IMP,ISS] [PMID 11375935];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016798 - hydrolase activity, acting
on glycosyl bonds [Evidence IEA]; GO_function: GO:0004571
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[Evidence IEA]; GO_function: GO:0004571 -
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[Evidence IMP] [PMID 19124653]; GO_process: GO:0030433 -
ER-associated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 11254655]; GO_process:
GO:0030433 - ER-associated ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 11375935];
GO_process: GO:0008152 - metabolic process [Evidence IEA];
GO_process: GO:0006986 - response to unfolded protein
[Evidence IEA]"
/codon_start=1
/product="alpha-1,2-mannosidase MNL1"
/protein_id="XP_018736211.1"
/db_xref="GeneID:30036835"
/translation="MRTGLFGLRSSAVVAAVSVVGGIGALGGVEAGEARAVNPSFSNE
FSRESLDSLKSEVRDLFYHGWTNYMDIAFPEDELMPLSCSGQSADLDDPMNMGLNDVL
GGYSVTLVDSLDMFAIMGDDDLFVQYVADVEKYVSFNVSSTVQVFETTIRGMGGLLSA
HLYATIPRLGHRINESEYNGHLLGLAYDLGERLLPAFDTVSGIPHPRVHLQEGVVPLP
GQSKLITETCTSGAGSMLLEFSLLSRLTGDPRFEKAARRAFFQLWARRASTNLVGITI
DAVSGNWQSVITGVGAGVDSFYEYALKYYVLFGDKSFFEVFETAYEALKKYSFDGWMF
HNVNFSTGIYITSWIDALGAFFPGLQVLAGDLTSAIKNHLVYYKLWNTYGGIPERWST
GIHDAIDSVVLEWYPLRPEFIESNYYLYQATKDPFYLQVGASTLRDLQRINKVACGVA
GTQDVRTGELSDRMESFFLSETVKYLYVLFDEENILNKEFSNYVYSTEAHPLWYDESV
LEYSSVERFPELASKIADNLEVLEIPRYELDLFTTHDESYNDDEDCSIRPSVMFQRIR
DIWQNRKREQLASYKIRPVKPEPQCTVWDTTNSGPFLQSSIASWNGFYTLDNFYDYTK
PDWITTNHPDRPDAIELVPEFHTNFVDSQSTCRAAVSSQVEILFAVPSGEASKTPYSV
GYNHGHPSLKGQHLVVALDLYGKRVRLERKENFVGPNSTFVITAVDDIDVKDVLLVHN
LDLSGNDARLIQMVNNTVLFQGFRIENILIDF"
gene <235127..>235852
/gene="RPS4A"
/locus_tag="AWJ20_4679"
/db_xref="GeneID:30036836"
mRNA <235127..>235852
/gene="RPS4A"
/locus_tag="AWJ20_4679"
/product="ribosomal 40S subunit protein S4A"
/transcript_id="XM_018881766.1"
/db_xref="GeneID:30036836"
CDS 235127..235852
/gene="RPS4A"
/locus_tag="AWJ20_4679"
/inference="similar to AA sequence:KEGG_Orthology:K02987"
/note="Protein component of the small (40S) ribosomal
subunit; mutation affects 20S pre-rRNA processing;
homologous to mammalian ribosomal protein S4, no bacterial
homolog; RPS4A has a paralog, RPS4B, that arose from the
whole genome duplication; GO_component: GO:0030686 - 90S
preribosome [Evidence IDA] [PMID 12150911]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0022627 - cytosolic small ribosomal
subunit [Evidence IDA] [PMID 6814480]; GO_component:
GO:0005622 - intracellular [Evidence IEA]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0005840 - ribosome [Evidence IEA,IEA];
GO_function: GO:0003723 - RNA binding [Evidence IEA,IEA];
GO_function: GO:0019843 - rRNA binding [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IEA]; GO_function: GO:0003735 -
structural constituent of ribosome [Evidence IDA] [PMID
6814480]; GO_process: GO:0002181 - cytoplasmic translation
[Evidence IC] [PMID 6814480]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="ribosomal 40S subunit protein S4A"
/protein_id="XP_018736212.1"
/db_xref="GeneID:30036836"
/translation="MLDKLSGTYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREVK
AILMQRLVKVDGKVRTDHTFPSGFMDVISLEKTGEHFRLVYDVKGRFAIHRITDEEAQ
YKLGKVKRVQLGKKGIPYLVTHDGRTIRYPDPEIKVNDTVKIDLTTGKISSFVKFENG
NVVMITGGRNLGRVGVITHRERHEGGFDLVHIKDSLDNSFVTRLSNVFVIGEGNKPLI
SLPKGKGIKLSIAEERDRRRAQA"
gene complement(<236320..>238320)
/locus_tag="AWJ20_4680"
/db_xref="GeneID:30036838"
mRNA complement(<236320..>238320)
/locus_tag="AWJ20_4680"
/product="hypothetical protein"
/transcript_id="XM_018881768.1"
/db_xref="GeneID:30036838"
CDS complement(236320..238320)
/locus_tag="AWJ20_4680"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736213.1"
/db_xref="GeneID:30036838"
/translation="MKVRFRCSGGWSAWGRLGLGFVVGFGLLGGSLAGGVKGDGTTES
EEGIRPLSWGHVNFVHTTDTHGWLPGHLSEPDFSANWGDYISFLQHMRELADEKGVDL
IVVDTGDRHDGNGLSDATYPDGEISQQIFRKADIDIVAIGNHELYKSEVTSQEYHTMR
PYYGEKYISSNIDVLDETGTGEWLPMGQRYRLFQTKNQNLTVLALGFLFNFKGNDYNS
RVNKVQDVIKEDWFQEVIRTPNLDLVVLTGHAPVRYFDEFDAVVKEIRSVWPKMPIQA
LGGHTHIRDYRIYDDYAVGLESGRFLETIGFASVNLSSEANVQQTVHSSQLDKTNKQN
EQESSESGFGSGFEDSVSSGPTFTRRYIDFNVRSLSTHAEKSLNEFYTAEGNATTQSI
IQFREQLNLTDIICCVPQTYYTTRAKYPGPDSLFTLLEEEVLPLLSGVVEPSRDDHPR
FILLNTGALRFDLFRGPFTVDTGYIVAPFRNEWIYIPSMPIKIARQILPLLNQEGSIL
STNEHATDFEELDFTELGPPEQRGRGSRNNLSSSQHRHNYDEQLVFSSEQQQQQHRQK
LTDGYVTHDDLGSDGDDTPHNPWPFYDIPNVIQASDNIDRTTTHVDLIFYDFMKPFIV
HALKTLRQDQYANNIVYYGGNSVISLLPEFLLGLETGTSCIV"
gene <240136..>241248
/gene="MGM101"
/locus_tag="AWJ20_4681"
/db_xref="GeneID:30036839"
mRNA <240136..>241248
/gene="MGM101"
/locus_tag="AWJ20_4681"
/product="Mgm101p"
/transcript_id="XM_018881769.1"
/db_xref="GeneID:30036839"
CDS 240136..241248
/gene="MGM101"
/locus_tag="AWJ20_4681"
/note="Protein with a role in mitochondrial DNA
recombinational repair; also involved in interstrand
cross-link repair; binds to and catalyzes the annealing of
single-stranded mtDNA; oligomerizes to form rings and
filaments; related to Rad52-type recombination proteins,
with limited overall similarity but sharing conserved
functionally important residues; component of the
mitochondrial nucleoid, required for the repair of
oxidative mtDNA damage; GO_component: GO:0000262 -
mitochondrial chromosome [Evidence IEA]; GO_component:
GO:0042645 - mitochondrial nucleoid [Evidence IEA,IEA];
GO_component: GO:0042645 - mitochondrial nucleoid
[Evidence IDA] [PMID 10209025]; GO_component: GO:0042645 -
mitochondrial nucleoid [Evidence IDA] [PMID 10869431];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003677 - DNA binding
[Evidence IEA]; GO_function: GO:0003697 - single-stranded
DNA binding [Evidence IDA,IMP] [PMID 22948312];
GO_process: GO:0006281 - DNA repair [Evidence IEA];
GO_process: GO:0006281 - DNA repair [Evidence IMP] [PMID
10209025]; GO_process: GO:0000733 - DNA strand
renaturation [Evidence IDA] [PMID 22027892]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEA]; GO_process: GO:0036297 - interstrand
cross-link repair [Evidence IGI] [PMID 22912599];
GO_process: GO:0000002 - mitochondrial genome maintenance
[Evidence IEA]; GO_process: GO:0000725 - recombinational
repair [Evidence IMP] [PMID 22027892]"
/codon_start=1
/product="Mgm101p"
/protein_id="XP_018736214.1"
/db_xref="GeneID:30036839"
/translation="MISRFRGGSATTGFVRGLKTATSYKSSVAVASRPVKSPAATRAM
APAATVRSGSGYSSARKSPAAGSGSSSSARYATAATSRSTRTATSRTKTAAAAPAATS
APAIDPAIFEGSSVGPSSGTIHSSKKSSSATPAEPLASNTIDSSGSASSAPPTFASSP
TSTTSSAANSAATRNSSPHIDLAATNSWTEFDDSPVDEGVENGENWTRSFRGLGSEPF
ASHVTDTLLAELKPEEIEITPDGLLFLPEIRYRRVLNRAFGPGGWGLAPRSKTLVTAK
SVSREYALICHGRLVAIARGEQDYFDPNGITTAMEGCKSNAMMRCCKDLGIASELWDP
FFIRDFKTKYCEQKYFEKKRRMVWKRKDREWEYPYK"
gene <241986..>243131
/locus_tag="AWJ20_4682"
/db_xref="GeneID:30036840"
mRNA <241986..>243131
/locus_tag="AWJ20_4682"
/product="hypothetical protein"
/transcript_id="XM_018881770.1"
/db_xref="GeneID:30036840"
CDS 241986..243131
/locus_tag="AWJ20_4682"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736215.1"
/db_xref="GeneID:30036840"
/translation="MVSISEQMARPPAPAAQSTAQAGPAVRQNTFGQGPGPQFRFSQG
VWAHEGSSTSGKKNRPPRAQQSSSGSGTGKGGSTKVKVDSLADIEAALKELEMGSTNH
EVCGCMATRHPLLEIAPNCLNCGRIICTKEGLGPCCFCGSPLISNEELLEINRVLQLE
KEQLTAGMSKKALRQAGIDPGKALSDMGLKPNKNEKSLEDAQRRLNTLLEFQESSAQR
TKIIDQVSDFETPFQGVNKWASPIEQAQQLKRQQHQLRKMHEQQLQRSGRGKKVISID
LKGNKVYMTESAEPLESEGEEDELSEGVSTEAQTNSTSDKDKKKPLNSWNPNSYGKSF
IKPVYRPVAGSGKRQPNSILKKIGIDEITGSHSRLNAEDDETRVLEM"
gene <244559..>244903
/locus_tag="AWJ20_4683"
/db_xref="GeneID:30036841"
mRNA <244559..>244903
/locus_tag="AWJ20_4683"
/product="hypothetical protein"
/transcript_id="XM_018881771.1"
/db_xref="GeneID:30036841"
CDS 244559..244903
/locus_tag="AWJ20_4683"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736216.1"
/db_xref="GeneID:30036841"
/translation="MSGTSMASPAVAGTIAMFMGMGDTVEAAMEKVTALATVDAIDRR
STLVKPRSPNLIAYNGLDLGIRKEYVDDDSANGSDDDTESLLNENLSTTEKIYSSLRN
LNGRSPANTTDI"
gene <247121..>247855
/gene="RCF2"
/locus_tag="AWJ20_4684"
/db_xref="GeneID:30036842"
mRNA <247121..>247855
/gene="RCF2"
/locus_tag="AWJ20_4684"
/product="Rcf2p"
/transcript_id="XM_018881772.1"
/db_xref="GeneID:30036842"
CDS 247121..247855
/gene="RCF2"
/locus_tag="AWJ20_4684"
/note="Cytochrome c oxidase subunit; has a role in
assembly of respiratory supercomplexes; similar to Rcf1p,
and either Rcf1p or Rcf2p is required for late-stage
assembly of the Cox12p and Cox13p subunits and for
cytochrome c oxidase activity; associates with the
cytochrome c oxidase - cytochrome bc1 supercomplex; null
mutant accumulates reactive oxygen species; member of the
conserved hypoxia induced gene family; C. elegans homolog
is functional in yeast; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0031305 - integral component of
mitochondrial inner membrane [Evidence IDA] [PMID
22342701]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0031966 - mitochondrial membrane
[Evidence IEA]; GO_component: GO:0097249 - mitochondrial
respiratory chain supercomplex [Evidence IDA] [PMID
22310663]; GO_component: GO:0097249 - mitochondrial
respiratory chain supercomplex [Evidence IDA] [PMID
22342701]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0033617 -
mitochondrial respiratory chain complex IV assembly
[Evidence IGI] [PMID 22310663]"
/codon_start=1
/product="Rcf2p"
/protein_id="XP_018736217.1"
/db_xref="GeneID:30036842"
/translation="MKFPSKEELNEYTHTIAVGAIKGAVIGTGVSALGYLYAKRRAPV
FFTYGAFPRTFLLIAPPIMIGVTTMEFASREFERERYGYVDAASASPDMLIPKINGSN
IDPKLAAKVGANGTDGAAAEPNAVLQYAAENKYKIIMGAWAASLAGSYWAVSKDKYLT
KSQRIVQARMYAQGLTVALLLVSVMLSVSNGKEKDNSRETGEADLREGKQTWEEIVEQ
ETARETAAHLPLHLSDRKNNGNHHHD"
gene complement(<248524..>249822)
/gene="TUF1"
/locus_tag="AWJ20_4685"
/db_xref="GeneID:30036843"
mRNA complement(<248524..>249822)
/gene="TUF1"
/locus_tag="AWJ20_4685"
/product="translation elongation factor Tu"
/transcript_id="XM_018881773.1"
/db_xref="GeneID:30036843"
CDS complement(248524..249822)
/gene="TUF1"
/locus_tag="AWJ20_4685"
/inference="similar to AA sequence:KEGG_Orthology:K02358"
/note="Mitochondrial translation elongation factor Tu;
comprises both GTPase and guanine nucleotide exchange
factor activities, while these activities are found in
separate proteins in S. pombe and humans; GO_component:
GO:0005622 - intracellular [Evidence IEA]; GO_component:
GO:0005759 - mitochondrial matrix [Evidence ISS] [PMID
6353412]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0005525 - GTP binding [Evidence IEA,IEA]; GO_function:
GO:0003924 - GTPase activity [Evidence IEA]; GO_function:
GO:0003924 - GTPase activity [Evidence IGI] [PMID
15695360]; GO_function: GO:0003924 - GTPase activity
[Evidence IDA] [PMID 3301847]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0003746
- translation elongation factor activity [Evidence
IEA,IEA]; GO_function: GO:0003746 - translation elongation
factor activity [Evidence IGI] [PMID 15695360];
GO_function: GO:0003746 - translation elongation factor
activity [Evidence IDA] [PMID 3301847]; GO_function:
GO:0003746 - translation elongation factor activity
[Evidence ISS] [PMID 6353412]; GO_process: GO:0006184 -
GTP catabolic process [Evidence IEA]; GO_process:
GO:0032543 - mitochondrial translation [Evidence IGI]
[PMID 15695360]; GO_process: GO:0032543 - mitochondrial
translation [Evidence IGI] [PMID 16777356]; GO_process:
GO:0070125 - mitochondrial translational elongation
[Evidence ISA] [PMID 3905388]; GO_process: GO:0006412 -
translation [Evidence IEA]; GO_process: GO:0006414 -
translational elongation [Evidence IEA,IEA,IEA]"
/codon_start=1
/product="translation elongation factor Tu"
/protein_id="XP_018736218.1"
/db_xref="GeneID:30036843"
/translation="MFGNLRLPGAIALRTVFSTASKSVVPRAAINSRWASTFQRTKPH
LNIGTIGHVDHGKTTLTAAITKVLASKGGANFLDYASIDKAPEERARGITITSAHVEY
ETDNRHYAHVDCPGHADYIKNMISGAARMDGAIIVVAASDGQMPQTREHLLLARQVGI
QHVVVFVNKVDTIDDPEMLELVEMEMRELLTTYGFDGDNTPVVMGSALCALEDKRPEI
GVQKIEELLDRIDEYIPTPSRDLDNAFLMPLESVFSIPGRGTVVTGRVERGVLSKGEE
VEIVGHHKEAITTVVTGIEMFKKELDKAQAGDNAGLLLRGVKREDLKRGMVIAKVGTV
KAHTKFLSSIYVLTKEEGGRHSPFGVNYRPQMFVSSADVTVTLTFPEGIEHDHQVFPG
DNTEMIGELVHPTPIEVGQRFNIREGGRTVGTGLVTRIIE"
gene <256171..>257940
/gene="PRC1"
/locus_tag="AWJ20_4686"
/db_xref="GeneID:30036844"
mRNA <256171..>257940
/gene="PRC1"
/locus_tag="AWJ20_4686"
/product="carboxypeptidase C PRC1"
/transcript_id="XM_018881774.1"
/db_xref="GeneID:30036844"
CDS 256171..257940
/gene="PRC1"
/locus_tag="AWJ20_4686"
/inference="similar to AA sequence:KEGG_Orthology:K13289"
/note="Vacuolar carboxypeptidase Y (proteinase C, CPY);
broad-specificity C-terminal exopeptidase involved in
non-specific protein degradation in the vacuole; member of
the serine carboxypeptidase family; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 11914276]; GO_component: GO:0000324 -
fungal-type vacuole [Evidence IDA] [PMID 14562095];
GO_component: GO:0000324 - fungal-type vacuole [Evidence
IDA] [PMID 23708375]; GO_component: GO:0005773 - vacuole
[Evidence IEA,IEA,IEA]; GO_function: GO:0004180 -
carboxypeptidase activity [Evidence IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0008233 - peptidase activity [Evidence
IEA]; GO_function: GO:0004185 - serine-type
carboxypeptidase activity [Evidence IEA]; GO_function:
GO:0004185 - serine-type carboxypeptidase activity
[Evidence IDA,IMP] [PMID 8679540]; GO_process: GO:0046938
- phytochelatin biosynthetic process [Evidence IDA,IMP]
[PMID 17408619]; GO_process: GO:0006508 - proteolysis
[Evidence IEA,IEA]; GO_process: GO:0007039 - vacuolar
protein catabolic process [Evidence TAS] [PMID 8789256]"
/codon_start=1
/product="carboxypeptidase C PRC1"
/protein_id="XP_018736219.1"
/db_xref="GeneID:30036844"
/translation="MKLNSGLTALAVLSSASALLVPPFTEKKEDSSSPSTQQFKFQIP
SELGSIDGLSSYLHIDRAQVASIMQTLAHELGQSWHDIPSLAIDSWYEVLKHSPQSFP
ELRENLVRQLELSRELNLPVSQVMDWGLKQSSSAHGGKAGVASSSSSSSNVKVDWEHV
VESDNFPAHSLNVKSPGSLGIDKVKQYSGYLNDNENDKHYFYWFFESRNDPKNDPVIL
WLNGGPGCSSLTGLLFENGPASISPELKPVHNPYSWNNNASVIFLDQPVNTGFSYSDS
STVSDTVTAGKDVYVFLSLFFQQFPEYNNLPFHIAGESYAGHYIPVFASEILSHDDRN
FNLSSVLIGNGLTDPLRQYDFYEPMACGKGGEPSVLSQEECDGMINSQPRCDNLINAC
YNSQSAWTCVPATVYCNNAMMGPYQRTGKNVYDIRANCEGGSLCYTALDDIDKYLNKP
EVKAALGAEVDEYASCNFDTNRNFVFAGDWMKPYQTSVTDLLEKDIPVLIYAGDKDFI
CNWLGNENWSKELEWSGGDKFKAAPLEPWKLADKEVGQVRNAGSHFTFLRIYGAGHMA
PYDQPEASLEMVNRWISGDFAFN"
gene complement(<258967..>259344)
/locus_tag="AWJ20_4687"
/db_xref="GeneID:30036845"
mRNA complement(<258967..>259344)
/locus_tag="AWJ20_4687"
/product="hypothetical protein"
/transcript_id="XM_018881775.1"
/db_xref="GeneID:30036845"
CDS complement(258967..259344)
/locus_tag="AWJ20_4687"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736220.1"
/db_xref="GeneID:30036845"
/translation="MDSSRLHNILDNIYIPKGPQESSNSIPNHHPTLIMQDYQTDTTH
HLKCGCCAGACIEENAWWNKKSSTTTPASKTTSTTTKKQAGQAAPRPSVMSTVSSSSI
ASASSNFSAYDSYSESSASDYEN"
gene complement(<262449..>264314)
/gene="TPD3"
/locus_tag="AWJ20_4688"
/db_xref="GeneID:30036846"
mRNA complement(<262449..>264314)
/gene="TPD3"
/locus_tag="AWJ20_4688"
/product="protein phosphatase 2A structural subunit TPD3"
/transcript_id="XM_018881776.1"
/db_xref="GeneID:30036846"
CDS complement(262449..264314)
/gene="TPD3"
/locus_tag="AWJ20_4688"
/inference="similar to AA sequence:KEGG_Orthology:K03456"
/note="Regulatory subunit A of the heterotrimeric PP2A
complex; the heterotrimeric protein phosphatase 2A (PP2A)
complex also contains regulatory subunit Cdc55p and either
catalytic subunit Pph21p or Pph22p; required for cell
morphogenesis and transcription by RNA polymerase III;
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 12388751]; GO_component: GO:0005934 - cellular
bud tip [Evidence IDA] [PMID 12388751]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 12388751];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 12388751]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 12388751]; GO_component: GO:0000159 -
protein phosphatase type 2A complex [Evidence IDA] [PMID
17550305]; GO_component: GO:0005816 - spindle pole body
[Evidence IDA] [PMID 12388751]; GO_function: GO:0004722 -
protein serine/threonine phosphatase activity [Evidence
IDA,IMP] [PMID 17550305]; GO_process: GO:0007094 - mitotic
spindle assembly checkpoint [Evidence IMP] [PMID 9001215];
GO_process: GO:0006417 - regulation of translation
[Evidence IMP] [PMID 10329624]"
/codon_start=1
/product="protein phosphatase 2A structural subunit TPD3"
/protein_id="XP_018736221.1"
/db_xref="GeneID:30036846"
/translation="MRRLSTISLALGKERTRTELVPFLEEVTQEDEDEVLTVLAEELG
GFIPYVGGPDNAHVLIPALEALSGAEEPVVRDKAVESLNLICQAFSTSQVKEYFLPLI
TRLSSTDWFTSRVSATGLYAAAISKLEGTERNDLLVLYKDLTQDEAPMVRRAAATSLP
KIIGELPELDFDENSTLHTMFISQITDDQDSVRLLSVDVLIAIAESLKKQSITKYNSE
LIGLTLDLFNDKSWRVRYMAADRYERLARSLDGSELREKFIPSFVKLMKDSEAEVRTA
ISKQIPGFCSLIPLAAIISEIIPNVELLAEDSSQHVRAALASEISRLAPLLEKEKTTQ
YLLPTFLQMLKDEFPDVRLNIISKLKIVNDVIGIELLSKSLLPAISELAKDKQWRVRL
AIIEYIPLLAEQLGVSFFDKELGPLCMTWLWDSVFSIREAATLNLRKLTEVFGVDWAK
DEIIQNVIAVAENSNYLYRLTALFAITTLIPVMDQAAITDSILPFIINLTDDAIPNIR
FNIAKSFKVLAETCLTWTDGGDEQDSATTTGPASPAAAKTDSASSTTEPSTGTSESTA
ATANKPVSPLAKQIITETIIPRLEKLTGDSDVDVRYFANKSLEEIDVLAKPLLEK"
gene <267515..>268207
/gene="NUO1"
/locus_tag="AWJ20_4689"
/db_xref="GeneID:30036847"
mRNA <267515..>268207
/gene="NUO1"
/locus_tag="AWJ20_4689"
/product="NADH dehydrogenase (ubiquinone) Fe-S protein 8"
/transcript_id="XM_018881777.1"
/db_xref="GeneID:30036847"
CDS 267515..268207
/gene="NUO1"
/locus_tag="AWJ20_4689"
/inference="similar to AA sequence:KEGG_Orthology:K03941"
/codon_start=1
/product="NADH dehydrogenase (ubiquinone) Fe-S protein 8"
/protein_id="XP_018736222.1"
/db_xref="GeneID:30036847"
/translation="MLSRSLRTNMGVSSPLRSVLMRQNGAGASASKMAVLRSCFHNVA
GGTEQGVPPVGFRVHRPPTWDESHESALDKATKFFLLSEMFRGMWVVLEQYFRAPYTI
YYPFEKGPVSPRFRGEHALRRYPSGEERCIACKLCEAICPALAITIEAEERVDGSRRT
TRYDIDMTKCIYCGYCQESCPVDAIVETPNVEYSTETREELLYNKEKLLANGDKWEQE
IQYGLDADAPYR"
gene complement(<275781..>276900)
/locus_tag="AWJ20_4690"
/db_xref="GeneID:30036849"
mRNA complement(join(<275781..276779,276898..>276900))
/locus_tag="AWJ20_4690"
/product="hypothetical protein"
/transcript_id="XM_018881778.1"
/db_xref="GeneID:30036849"
CDS complement(join(275781..276779,276898..276900))
/locus_tag="AWJ20_4690"
/inference="similar to AA sequence:KEGG_Orthology:K09142"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736223.1"
/db_xref="GeneID:30036849"
/translation="MIARAAVLYGASEIVIFDVANVSGSDAVDQDGDTVVSDSVVEKP
KKVVFDEAGESSESKKDAKKESEGPSPECIRLASLLQYYITPSYLRNLLFTDLKPLQY
AKKLPKIPGLPFLSHSSSQFLEGLTVAGKLPRSATGGVKKKKGSRKSKKQLQLEASTE
YVQIGEQNVLKLDNQRVPLGTRVTVDTKSKKVVSPKDAYTSSSGVLFTNKDSFGYTVR
IAKTFGKIFSESIYPGGYTYTAYVPSEEFVASSATAYEPIRPIVDKKGTIFDGAKSSS
HILLVYGRWADVNKAVQNDKEELNELDDATGIFDGRFAIRASGRTEDAILISLAQLE"
gene complement(<278236..>279282)
/locus_tag="AWJ20_4691"
/db_xref="GeneID:30036850"
mRNA complement(<278236..>279282)
/locus_tag="AWJ20_4691"
/product="hypothetical protein"
/transcript_id="XM_018881779.1"
/db_xref="GeneID:30036850"
CDS complement(278236..279282)
/locus_tag="AWJ20_4691"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736224.1"
/db_xref="GeneID:30036850"
/translation="MGPLQRARVYDWSKEFLIVLSIMGYVVLHFLGLRVNQGKVSSWI
KAHLDVLQTQFYQVGPKPNITSTKSIDYIVTDGATAYNTYATGRVNVSHVLAKFRLLG
RQNFITLSMEYFTSIFLQGFNPRDQIDVVITPSDPAAIDPFVFAVVNKENMSRSRDQN
YFLSITRTVDSDKLPPSFTFMTESAEVTDTVVELAKEFFQALETPGVEKVLEYFAITD
QQPTAPLSLKDLEPKTKLYLSLKFPSNKEQSELSARILNSAINLVDILVAKKTWRPEV
AKKIKATRDAEIKKVQKALDLIKAEELAQKKTEALREKKAALAKLSPEEQKKLDQKSK
EKEQRKLRSKQIRR"
gene complement(<280105..>280569)
/locus_tag="AWJ20_4692"
/db_xref="GeneID:30036851"
mRNA complement(<280105..>280569)
/locus_tag="AWJ20_4692"
/product="hypothetical protein"
/transcript_id="XM_018881780.1"
/db_xref="GeneID:30036851"
CDS complement(280105..280569)
/locus_tag="AWJ20_4692"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736225.1"
/db_xref="GeneID:30036851"
/translation="MKVAPGALVCRWFALDGYCDTGDKCDKRHVFECPDFEATGQCHR
KKCPLKHILRAKEEGKFETHDVTNTTEDSSLPIDLDAVIDMINAEDESPDDDSDNDES
ENDDDGSNDDESGSDDDDSADDVSSDDSDVMQWDFTHNNHLPDDDNEDFISI"
gene complement(<281151..>281639)
/locus_tag="AWJ20_4693"
/db_xref="GeneID:30036852"
mRNA complement(<281151..>281639)
/locus_tag="AWJ20_4693"
/product="hypothetical protein"
/transcript_id="XM_018881781.1"
/db_xref="GeneID:30036852"
CDS complement(281151..281639)
/locus_tag="AWJ20_4693"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736226.1"
/db_xref="GeneID:30036852"
/translation="MTRPYHSNSYRGRGSGYRGGYRGAHRGGYHHHQSTGPTHQWKRV
EESSSQTTSSTTTSGGTGISTGSNTTSATSSTPIKAPKPVANSDGTVDIGGVKYRTAK
NGSKLIRVDPSEQPKESTPRKTTIQGVSYVRTKSGNLLRKNPLSNDRCVDHSLTYYLA
CF"
gene complement(<284118..>285305)
/gene="MTM1"
/locus_tag="AWJ20_4694"
/db_xref="GeneID:30036853"
mRNA complement(<284118..>285305)
/gene="MTM1"
/locus_tag="AWJ20_4694"
/product="Mtm1p"
/transcript_id="XM_018881782.1"
/db_xref="GeneID:30036853"
CDS complement(284118..285305)
/gene="MTM1"
/locus_tag="AWJ20_4694"
/inference="similar to AA sequence:KEGG_Orthology:K15119"
/note="Mitochondrial protein of the mitochondrial carrier
family; high affinity pyridoxal 5'-phosphate transporter;
involved in mitochondrial iron homeostasis and in
activating mitochondrial Sod2p by facilitating insertion
of an essential manganese cofactor; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 12890866]; GO_function: GO:0016530 -
metallochaperone activity [Evidence IMP] [PMID 12890866];
GO_function: GO:0030170 - pyridoxal phosphate binding
[Evidence IDA] [PMID 24184947]; GO_process: GO:0006828 -
manganese ion transport [Evidence IMP] [PMID 12890866];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Mtm1p"
/protein_id="XP_018736227.1"
/db_xref="GeneID:30036853"
/translation="MVAACSGSLLTSVVVTPFDVVRVRMQQQSAFPMVDFIDLAKNKV
ANASNKGVEISYKAAAGQVRNNLSKVAVSSLESLRLSSSTGVAAAEMAVKSFPITQIP
SGVGVTTCCKDVFWFPSTIDYCVASQLEISTSCAVDETKMNRLNGTWDGFRKIVAGEG
LPALWRGLSLTLLMSVPSNVVYFIAYEYMRDNSPLSESTILNPLICGGLARSLSATVV
SPIELVKTRLQSAKGPDAVQVVTKGVREMVATQGFSSLWRGLVLTLWRDVPFSSIYWA
CVEFIRAELAQTHYFRDQKSDSSTFSQAFIAGSVGGSIAAILTTPFDVGKTRRQIGHH
ASSSSSMGMLPFMMKILKTEGVGALYVGAIPRILKVSPACAIMISSYEMGKKFFARDN
NAL"
gene <287332..>291078
/gene="KOG1"
/locus_tag="AWJ20_4695"
/db_xref="GeneID:30036854"
mRNA <287332..>291078
/gene="KOG1"
/locus_tag="AWJ20_4695"
/product="Kog1p"
/transcript_id="XM_018881783.1"
/db_xref="GeneID:30036854"
CDS 287332..291078
/gene="KOG1"
/locus_tag="AWJ20_4695"
/inference="similar to AA sequence:KEGG_Orthology:K07204"
/note="Subunit of TORC1; TORC1 is a rapamycin-sensitive
complex involved in growth control that contains Tor1p or
Tor2p, Lst8p and Tco89p; contains four HEAT repeats and
seven WD-40 repeats; may act as a scaffold protein to
couple TOR and its effectors; GO_component: GO:0031931 -
TORC1 complex [Evidence IEA]; GO_component: GO:0031931 -
TORC1 complex [Evidence IPI] [PMID 12408816];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IDA] [PMID 14562095]; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
16394584]; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 17560372]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005886 - plasma
membrane [Evidence IEA,IEA]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 16394584];
GO_component: GO:0005774 - vacuolar membrane [Evidence
IEA]; GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0043130 - ubiquitin binding [Evidence IDA]
[PMID 21070969]; GO_process: GO:0031929 - TOR signaling
[Evidence IEA]; GO_process: GO:0031929 - TOR signaling
[Evidence IC] [PMID 12408816]; GO_process: GO:0009267 -
cellular response to starvation [Evidence IMP] [PMID
12408816]; GO_process: GO:0001558 - regulation of cell
growth [Evidence IPI] [PMID 12408816]"
/codon_start=1
/product="Kog1p"
/protein_id="XP_018736228.1"
/db_xref="GeneID:30036854"
/translation="MNDPKKSLEMIGKALQSQYEALSTRTKYKQSLDPNVEDAKRFCM
SLRRSARDERILFHYNGHGVPRPTPSGEIWVFNRGYTQYIPVSVYDLQTWLGAPCIYA
YDCHAAGHIVNNFNAFVEKRKENEKKNGPDPNGPPAEAYENCIQLAACRADEVLPMHP
ELPADLFTCCITSPIDIAVRWFVMQSPLLRGQFDNLSIPGKITDRRTPLGELNWIFTA
ITDTIAWSTLEGPLFKRLFRQDLVVAAMFRNFLLAVRIMRVHNCHPVSSPELPDTHNH
PMWDAWDLAVDHCLVQLPMLQAASEPGGVPYTYKHSDFFEQQLTAFEVWLKYKSSDLD
KPPEQLPVLLQVLLSQIHRLRALVLLSKYLDLGPKAVHLALSIGIFPYVLKLLQSPAP
ELKPVLVFIWARIMAVDHKAIKQELVKENGYAYFINILIPQDGPVVNAINVPEHLAMC
AFVTALFCEGYKQGQRLCMGPDLFQACLTHTEELESPLLRQWATLCISQLWDGYPEAK
WQALVKDKTALDRLSRLLNDPVPEVRTACIVALTTFLGDGETVNPNSPNINATAVTSG
LTHGDIRQKELSFAISVLALTNDASSIVRREVVVFYSKFVKQYRSRFLVAAYSSLEED
VAILRSQTNIDDIRANSPAHGSIYTVVWRALLVLTEDPFPEVADYAQDVVDYVFQLLR
KTPLSAEVEKLEQYLVQYSMSSLSVNLPELQNNSPNKATRVSSMGPSAVAGSGVGHGS
HNGPTSESKFSQTLLKRSVSFANTLKQYAWGSGEEEQLQSQNQHPQHGRAGNGSASGS
NTLWGGFGGSFVNSTGAGAGNLGNTRSAFPTVTTVAYGVGRRPRPVRYQTKDYTKKIV
LPLASGFFEWSCEYFQEPQMCNTESDEPGSERYLERVWRKSRNEHIIAETQVQKEMAV
YGGWSTHVGLLNNLTQPTKLEFAQFEPHLVGVDDRDGVTVWDWTESTKLNRFCNSNPV
GTRITEAKFLNEDDVPLLLTGSSEGVVRIYRHYESVKNIELACSWRVLTDLLPAHRNS
GLIAEWQQSRGALLVGGDVRVIRVWDAPRELCTSDIPARSGSPVTALTSDQVAGNIVV
AGFGDGGIRVYDRRLGPRESLVKTWKRHKSWVVNVRMQRGGARELVSGSTDGQVYLWD
IRLDEPVLSFQAHSNGMRAIDVHEHAPVIATGSQTVGIWSTNGNRVATVRPPSSGYSL
GSKASQVSALSFHPHRMIMAVNNVQDAHIGVFNCTDDPSRKYGR"
gene complement(<291263..>292501)
/gene="LTV1"
/locus_tag="AWJ20_4696"
/db_xref="GeneID:30036855"
mRNA complement(<291263..>292501)
/gene="LTV1"
/locus_tag="AWJ20_4696"
/product="Ltv1p"
/transcript_id="XM_018881784.1"
/db_xref="GeneID:30036855"
CDS complement(291263..292501)
/gene="LTV1"
/locus_tag="AWJ20_4696"
/inference="similar to AA sequence:KEGG_Orthology:K14798"
/note="Component of the GSE complex; GSE is required for
proper sorting of amino acid permease Gap1p; required for
ribosomal small subunit export from nucleus; required for
growth at low temperature; GO_component: GO:0034448 - EGO
complex [Evidence IDA] [PMID 16732272]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
16888326]; GO_component: GO:0031902 - late endosome
membrane [Evidence IDA] [PMID 16732272]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 16888326];
GO_component: GO:0030688 - preribosome, small subunit
precursor [Evidence IDA] [PMID 16738661]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0034599 - cellular response to oxidative stress
[Evidence IMP] [PMID 15611164]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0006970 -
response to osmotic stress [Evidence IMP] [PMID 15611164];
GO_process: GO:0042274 - ribosomal small subunit
biogenesis [Evidence IMP] [PMID 15611164]; GO_process:
GO:0000056 - ribosomal small subunit export from nucleus
[Evidence IGI,IMP] [PMID 16888326]; GO_process: GO:0006810
- transport [Evidence IEA]"
/codon_start=1
/product="Ltv1p"
/protein_id="XP_018736229.1"
/db_xref="GeneID:30036855"
/translation="MGPKRWIDKKKADTYSLVYRSQEDPLINDPDASQRVLVKLDNLN
DAKQKQERRERWAPTPSYSSTGGASKVLTADVLAQDEETFRKEGRRDNEGEAALYGIE
YDDSKYDYMQHLKPIGEDPSAVYISKKPTKEKQSFGKDGNLLLKSDYAAANKVNLPSD
VLPSKDTLKRTYQDQQNIPDEIAGLQPDMDPRLREVLEALEDEEYVDDDEDVFGQLIS
SGQKEEIEEDEDEWADYDPDQDFSGQDYSDDEAYDSDNGREMPAKPAKYMVTAKEGEE
DWETAFRQFKLDQSRSKAPIDGVSDDEFDEDRDEVGSLSQVPSGRRITKSGRKQKIGA
KTDLTGFSMSSSALFRNDGLTLLDNRFDKIEEEYNNDDDEEEEANEPFDMSKERDDLE
AIMDDFLDNYVVEGKKMYKK"
gene complement(<292889..>295288)
/gene="AVT3"
/locus_tag="AWJ20_4697"
/db_xref="GeneID:30036856"
mRNA complement(<292889..>295288)
/gene="AVT3"
/locus_tag="AWJ20_4697"
/product="Avt3p"
/transcript_id="XM_018881785.1"
/db_xref="GeneID:30036856"
CDS complement(292889..295288)
/gene="AVT3"
/locus_tag="AWJ20_4697"
/inference="similar to AA sequence:KEGG_Orthology:K14209"
/note="Vacuolar transporter; exports large neutral amino
acids from the vacuole; member of a family of seven S.
cerevisiae genes (AVT1-7) related to vesicular
GABA-glycine transporters; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IMP] [PMID
11274162]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005774 - vacuolar membrane
[Evidence IEA]; GO_component: GO:0005773 - vacuole
[Evidence IEA]; GO_function: GO:0015186 - L-glutamine
transmembrane transporter activity [Evidence IGI,IMP]
[PMID 11274162]; GO_function: GO:0015188 - L-isoleucine
transmembrane transporter activity [Evidence IGI,IMP]
[PMID 11274162]; GO_function: GO:0005302 - L-tyrosine
transmembrane transporter activity [Evidence IGI,IMP]
[PMID 11274162]; GO_function: GO:0005215 - transporter
activity [Evidence ISS] [PMID 11274162]; GO_process:
GO:0032974 - amino acid transmembrane export from vacuole
[Evidence IGI,IMP] [PMID 11274162]; GO_process: GO:0006865
- amino acid transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Avt3p"
/protein_id="XP_018736230.1"
/db_xref="GeneID:30036856"
/translation="MSSPKPISSSPKSANDSTNSRRGSMAGSPFRQASLRLGSPVTVG
SFSQRSLGPGTGASPAAVSDFDDNVFSTDRRSFSLQRKSFGNAINGGVSSGLATSLST
SNAIGNSNGNGTGNGSGNGTLSRAMSSSSASPSNLLGSNIPGSSAVSISDQGSSIIDI
DNPDPEIVKVVGRHLVREARGSDFGSDYDGSVGLGSNDGLTAGTSNGAGGNGTAGIGN
RATDVVDDDDKFMSLRLQGGDITRQLYNWQREHEEGDAATKRGRSRSFDLPRPAIVGP
DGHPIDTKNIKAPGGFRRNFISHNHQTRNRHDPESQNTRRPAFLTRNFIEFLSIYGHF
AGEELEDDEDEDDEEDGAGSPFGYGGIGGASVGGSGGAGSGGNGNGDEEQDQDETSAL
ISRPRLSRKKSAHHGPGNATQTKAVMLLLKSFVGTGVLFLPKAYSNGGLLFCSVLLVF
VSLISYWCFLLLIESKNRVGGGSFGDIGGVLYGPRMRQLILFSIVISQIGFAAAYIVF
VSENLQAFVMAVTNNQTFIRVEVFIFLQLIVFLPLSMIRDIAKLSGTALVADFFILLG
LVYLYYYGAHTIAEQGVADVVMFNSESWTLFIGTAIFTYEGIGLIIPIQESMKHPQKF
TPVLAGVMVGITLVFVSMGALQYAAYGTKVKTVIILNLPQDSHFVNGVQFLYSAAILL
STPLQLFPAIRIMENWLFVRSGKYNTKIKWQKNVFRFFLTFLTALVAWGGADDLDKFV
ALIGSFACIPLVYIYPPLLHLKAVKQSWYMVLADVVLAVAGLGAMIYTTAGTVSSWIK
S"
gene complement(<296807..>297172)
/gene="RPC25"
/locus_tag="AWJ20_4698"
/db_xref="GeneID:30036857"
mRNA complement(<296807..>297172)
/gene="RPC25"
/locus_tag="AWJ20_4698"
/product="DNA-directed RNA polymerase III subunit RPC25"
/transcript_id="XM_018881786.1"
/db_xref="GeneID:30036857"
CDS complement(296807..297172)
/gene="RPC25"
/locus_tag="AWJ20_4698"
/inference="similar to AA sequence:KEGG_Orthology:K03022"
/note="RNA polymerase III subunit C25; required for
transcription initiation; forms a heterodimer with Rpc17p;
paralog of Rpb7p; GO_component: GO:0005666 - DNA-directed
RNA polymerase III complex [Evidence IDA] [PMID 10611227];
GO_component: GO:0005666 - DNA-directed RNA polymerase III
complex [Evidence IDA] [PMID 3905793]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence IEA]; GO_function:
GO:0003899 - DNA-directed RNA polymerase activity
[Evidence IEA,IEA]; GO_function: GO:0001056 - RNA
polymerase III activity [Evidence IDA] [PMID 3905793];
GO_process: GO:0042797 - tRNA transcription from RNA
polymerase III promoter [Evidence IMP] [PMID 15612920];
GO_process: GO:0042797 - tRNA transcription from RNA
polymerase III promoter [Evidence IDA] [PMID 3905793];
GO_process: GO:0042797 - tRNA transcription from RNA
polymerase III promoter [Evidence IMP] [PMID 8065349];
GO_process: GO:0006384 - transcription initiation from RNA
polymerase III promoter [Evidence IMP] [PMID 15612920];
GO_process: GO:0006351 - transcription, DNA-templated
[Evidence IEA]"
/codon_start=1
/product="DNA-directed RNA polymerase III subunit RPC25"
/protein_id="XP_018736231.1"
/db_xref="GeneID:30036857"
/translation="MIVFRPFVGEILVGWVSSCTEEGINVKMEFFDDIHIPKSLLFEE
CSFVPREQAWLWKTEESELYIDTNEKIRFRVEQEIFSNQPPKRPGVEEEEQVHNQVPP
YSIIGSCQTDGMGLVSWWE"
gene <299330..>301162
/gene="SDH1"
/locus_tag="AWJ20_4699"
/db_xref="GeneID:30036858"
mRNA <299330..>301162
/gene="SDH1"
/locus_tag="AWJ20_4699"
/product="succinate dehydrogenase flavoprotein subunit
SDH1"
/transcript_id="XM_018881787.1"
/db_xref="GeneID:30036858"
CDS 299330..301162
/gene="SDH1"
/locus_tag="AWJ20_4699"
/inference="similar to AA sequence:KEGG_Orthology:K00234"
/note="Flavoprotein subunit of succinate dehydrogenase;
couples the oxidation of succinate to the transfer of
electrons to ubiquinone as part of the TCA cycle and the
mitochondrial respiratory chain; FAD binding to Sdh1p is
required for the assembly of the succinate dehydrogenase
subunits; SDH1 has a paralog, YJL045W, that arose from the
whole genome duplication; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0031966 - mitochondrial membrane
[Evidence IDA] [PMID 1939170]; GO_component: GO:0005749 -
mitochondrial respiratory chain complex II [Evidence IDA]
[PMID 9822678]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 2826438]; GO_function:
GO:0050660 - flavin adenine dinucleotide binding [Evidence
IEA]; GO_function: GO:0050660 - flavin adenine
dinucleotide binding [Evidence IDA] [PMID 18279395];
GO_function: GO:0050660 - flavin adenine dinucleotide
binding [Evidence IMP] [PMID 1939170]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA,IEA];
GO_function: GO:0016627 - oxidoreductase activity, acting
on the CH-CH group of donors [Evidence IEA]; GO_function:
GO:0008177 - succinate dehydrogenase (ubiquinone) activity
[Evidence IEA]; GO_function: GO:0008177 - succinate
dehydrogenase (ubiquinone) activity [Evidence IDA] [PMID
13445213]; GO_function: GO:0008177 - succinate
dehydrogenase (ubiquinone) activity [Evidence IGI,IMP]
[PMID 16232921]; GO_function: GO:0008177 - succinate
dehydrogenase (ubiquinone) activity [Evidence IMP] [PMID
197391]; GO_function: GO:0008177 - succinate dehydrogenase
(ubiquinone) activity [Evidence IMP] [PMID 9730279];
GO_process: GO:0045333 - cellular respiration [Evidence
IGI,IMP] [PMID 16232921]; GO_process: GO:0045333 -
cellular respiration [Evidence IGI,IMP] [PMID 9730279];
GO_process: GO:0022900 - electron transport chain
[Evidence IEA]; GO_process: GO:0006121 - mitochondrial
electron transport, succinate to ubiquinone [Evidence IDA]
[PMID 2826438]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0006099 - tricarboxylic acid cycle
[Evidence IEA,IEA,IEA]; GO_process: GO:0006099 -
tricarboxylic acid cycle [Evidence IC] [PMID 13445213]"
/codon_start=1
/product="succinate dehydrogenase flavoprotein subunit
SDH1"
/protein_id="XP_018736232.1"
/db_xref="GeneID:30036858"
/translation="MKPIRAQEASKHVSSKYPVIDHEYDAIVVGAGGAGLRAAFGLAE
AGFNTACISKLFPTRSHTVAAQGGINAALGNMHPDNWKWHMYDTVKGSDWLGDQDAIH
YMTKEAPQSIIELEHYGVPFSRNDEGRIYQRAFGGQSKEFGKGGQAYRTCAVADRTGH
ALLHSLYGQSLRHNTNFFIEFFAMDLLMQDGECVGVMAYNQEDGTLHRFRAHKTVLAT
GGYGRAYFSCTSAHTCTGDGLAMVTRAGLPLQDLEFVQFHPTGIYGSGCLITEGSRGE
GGYLLNKNGERFMERYAPTAKDLASRDVVSRSMTLEIREGRGVGPENDHIYLQLSHLP
AEVLKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTKWTGEALTVDENGND
KVVPGLYACGEAACVSVHGANRLGANSLLDLVVFGRAVAHTIRDQLTPGTPLKPVSAD
IGHESIANLDKVRTATGSKSTAEIRLNMQKVMQTDVSVFRTQESLEDGVRRITEVDKS
IVDLGTTDRSMIWNSDLVESLELQNLLTCAMQTAYSAVARKESRGAHAREDYPERDDV
NWMKHTLSWQDKPGDPVKLGYRNVISTTLDEAECPSVPPAKRVY"
gene <301515..>302012
/gene="PSF1"
/locus_tag="AWJ20_4700"
/db_xref="GeneID:30036861"
mRNA <301515..>302012
/gene="PSF1"
/locus_tag="AWJ20_4700"
/product="Psf1p"
/transcript_id="XM_018881790.1"
/db_xref="GeneID:30036861"
CDS 301515..302012
/gene="PSF1"
/locus_tag="AWJ20_4700"
/inference="similar to AA sequence:KEGG_Orthology:K10732"
/note="Subunit of the GINS complex (Sld5p, Psf1p, Psf2p,
Psf3p); complex is localized to DNA replication origins
and implicated in assembly of the DNA replication
machinery; GO_component: GO:0031261 - DNA replication
preinitiation complex [Evidence IGI,IMP] [PMID 12730134];
GO_component: GO:0000811 - GINS complex [Evidence IPI]
[PMID 12730134]; GO_component: GO:0000811 - GINS complex
[Evidence IPI] [PMID 12768207]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0031298 -
replication fork protection complex [Evidence IDA] [PMID
16531994]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006260 - DNA replication
[Evidence IEA]; GO_process: GO:0006261 - DNA-dependent DNA
replication [Evidence IMP] [PMID 12730134]; GO_process:
GO:0006261 - DNA-dependent DNA replication [Evidence IMP]
[PMID 12768207]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0000727 - double-strand
break repair via break-induced replication [Evidence IMP]
[PMID 20516198]"
/codon_start=1
/product="Psf1p"
/protein_id="XP_018736233.1"
/db_xref="GeneID:30036861"
/translation="MYGDIANKLIQDAKLTQMLETLPLYQEELVRSIVREVRDLHRDT
EAVLARYGGELPTQDRKVACWIFVQHLCMRRNKRCLLAYQRLRSTRLDAMAWNDVDTM
DQEVLNNLNPNEQDYLRDYSELLVDYKGEWVDVDLTGSLEPPRDLFIDVRVLKDAGEI
QTEYG"
gene <303013..>304329
/gene="RPT1"
/locus_tag="AWJ20_4701"
/db_xref="GeneID:30036862"
mRNA <303013..>304329
/gene="RPT1"
/locus_tag="AWJ20_4701"
/product="proteasome regulatory particle base subunit
RPT1"
/transcript_id="XM_018881791.1"
/db_xref="GeneID:30036862"
CDS 303013..304329
/gene="RPT1"
/locus_tag="AWJ20_4701"
/inference="similar to AA sequence:KEGG_Orthology:K03061"
/note="ATPase of the 19S regulatory particle of the 26S
proteasome; one of six ATPases of the regulatory particle;
involved in the degradation of ubiquitinated substrates;
required for optimal CDC20 transcription; interacts with
Rpn12p and Ubr1p; mutant has aneuploidy tolerance;
GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0000502 - proteasome complex
[Evidence IEA]; GO_component: GO:0008540 - proteasome
regulatory particle, base subcomplex [Evidence IDA] [PMID
9741626]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0016887 - ATPase activity
[Evidence ISS] [PMID 7754704]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0017111
- nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_process: GO:0045899 - positive regulation of
RNA polymerase II transcriptional preinitiation complex
assembly [Evidence IMP] [PMID 19843524]; GO_process:
GO:0045732 - positive regulation of protein catabolic
process [Evidence IDA] [PMID 15135049]; GO_process:
GO:0070682 - proteasome regulatory particle assembly
[Evidence IMP] [PMID 19412160]; GO_process: GO:0030163 -
protein catabolic process [Evidence IEA]; GO_process:
GO:0006511 - ubiquitin-dependent protein catabolic process
[Evidence IPI] [PMID 15135049]"
/codon_start=1
/product="proteasome regulatory particle base subunit
RPT1"
/protein_id="XP_018736234.1"
/db_xref="GeneID:30036862"
/translation="MPPKADWEKYKAPIDGDDEKPEENIVPLSEGDIQVLKTYGAAPY
GSALKVIEKDIKDIQGRINEKVGVKESDTGLAAPHLWDIMADKQTISQEQPLQVARCT
KIIQSPEDPEKAKYVINVKQIAKFVVSLGDQVSPTDIEEGMRVGVERNKYQIMLPLPP
RIDPSVTMMTVEEKPDVTYGDIGGSKEQIEKLREVVELPLLSPERFANLGIDPPKGIL
LYGPPGTGKTLCARAAANRTDATFIRVIGSELVQKYVGEGARMIRELFEMARTKKACI
IFFDEIDAIGGARFDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVMFATNRPNTLDP
ALLRPGRIDRKVEFSLPDIEGRANIFRIHSKSMSVDRDIRWELISRLCPNSTGAELRS
VCTEAGMFAIRARRKVATEKDFLAAVDKVIKGNLKFSSTSQYMQYN"
gene <304726..>304797
/locus_tag="AWJ20_4702"
/db_xref="GeneID:30036863"
tRNA <304726..>304797
/locus_tag="AWJ20_4702"
/product="tRNA-Gln"
/db_xref="GeneID:30036863"
gene complement(<304987..>306528)
/gene="DBR1"
/locus_tag="AWJ20_4703"
/db_xref="GeneID:30036864"
mRNA complement(<304987..>306528)
/gene="DBR1"
/locus_tag="AWJ20_4703"
/product="Dbr1p"
/transcript_id="XM_018881792.1"
/db_xref="GeneID:30036864"
CDS complement(304987..306528)
/gene="DBR1"
/locus_tag="AWJ20_4703"
/inference="similar to AA sequence:KEGG_Orthology:K18328"
/note="RNA lariat debranching enzyme; catalyzes
debranching of lariat introns formed during pre-mRNA
splicing; required for efficient Ty1 transposition;
knockdown of human homolog Dbr1 rescues toxicity of
RNA-binding proteins TDP-43 and FUS which are implicated
in amyotrophic lateral sclerosis (ALS), suggests potential
therapeutic target for ALS and related TDP-43
proteinopathies; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IC] [PMID 1850323]; GO_function: GO:0008419 -
RNA lariat debranching enzyme activity [Evidence IDA,IMP]
[PMID 1850323]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA,IEA]; GO_function: GO:0016788 -
hydrolase activity, acting on ester bonds [Evidence IEA];
GO_process: GO:0006401 - RNA catabolic process [Evidence
IDA,IMP] [PMID 1850323]; GO_process: GO:0006397 - mRNA
processing [Evidence IEA,IEA]; GO_process: GO:0000398 -
mRNA splicing, via spliceosome [Evidence IMP] [PMID
24919400]; GO_process: GO:0007124 - pseudohyphal growth
[Evidence IMP] [PMID 9055077]; GO_process: GO:0016074 -
snoRNA metabolic process [Evidence IMP] [PMID 9740128];
GO_process: GO:0032197 - transposition, RNA-mediated
[Evidence IMP] [PMID 10652222]"
/codon_start=1
/product="Dbr1p"
/protein_id="XP_018736235.1"
/db_xref="GeneID:30036864"
/translation="MMRKKSFNIVVEGCCHGELDSIYEAIGKLNKPVDLVLICGDFQA
LRNEQDMNCIAMPPKYRKLGDFHKYYTGEKKAPYLTLFIGGNHESSNYLWELFYGGWA
APNIYYMGAASVVNFNGLKIGGLSGIYNHQHFHWHHLEKLPYNGSSERSVYHVRKFDT
MKLLMLRQGLNVMMSHDWPARIEHFGDLKKLLRFKKHLARDVDTGRLGSPAAGSILQN
VRPDYWFSAHLHVRYEALVKHEPHEEIVNETKNATKLIEEESKNEDEIDLEELLDETD
NDKKNEDEIELEGLLDETANDNEANKSNVLHAKEETFQKEALNSDEIDLSSELSLEPS
TDQPVKSEAPNNVNKVGAPETHFLALDKCLPGRRFIEHITIEVETRPEKRKRRPSTLD
DGDSAQVTEEETGLSYDPEWLAITRAFHKYFKTSSNQTVDELPHVNDIDSLNEHISTE
LQWVNENIVDKDLLTIPNNFKVQTETPAAPTSSWKNQQPPEIRNNQTEDFCRLLELDN
RIYHL"
gene <307101..>308591
/gene="BFR2"
/locus_tag="AWJ20_4704"
/db_xref="GeneID:30036865"
mRNA <307101..>308591
/gene="BFR2"
/locus_tag="AWJ20_4704"
/product="Bfr2p"
/transcript_id="XM_018881793.1"
/db_xref="GeneID:30036865"
CDS 307101..308591
/gene="BFR2"
/locus_tag="AWJ20_4704"
/inference="similar to AA sequence:KEGG_Orthology:K14782"
/note="Essential protein that is a component of 90S
preribosomes; may be involved in rRNA processing;
multicopy suppressor of sensitivity to Brefeldin A;
expression is induced during lag phase and also by cold
shock; GO_component: GO:0030686 - 90S preribosome
[Evidence IDA] [PMID 12150911]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 15590835];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0015031 - protein transport [Evidence
IEA]; GO_process: GO:0006364 - rRNA processing [Evidence
IMP] [PMID 15590835]; GO_process: GO:0006810 - transport
[Evidence IEA]; GO_process: GO:0016192 - vesicle-mediated
transport [Evidence IEA]"
/codon_start=1
/product="Bfr2p"
/protein_id="XP_018736236.1"
/db_xref="GeneID:30036865"
/translation="MPSLADQLAKLANPEPVDFDPEDFENRNPDDDSGNELEVNKAAT
EHYLEVSKSSLRNDELNVDGVKYVGKRVKRDALFEQNGGHSEESDDDDEEEVEGEDDS
EEEEEEEEDSEENDVVNGNESEEEDEMQSDDDISEKKEQRKLNGSASDGVPEDEVAKR
AQLQELIDAERKNLAGRLSNTAKADAEKGSAVQHQLNIYESIIDCRIKLQKSIQAVNE
LPISRSSAEKFTTEATPELISKNKESVFDLLNKVSQLRLRLLQKDGLTDLPKKLSKKR
SLTTAFENAQSLDKPLYGYRESVLTKWSQKVQSSTGSMALQSSKFKSLNQTAAIQVST
VLADMERLVKRTKLNRSNVKALGLETVAEDQEKQAKTVDIQEEDHIFDDTDFYRQILK
ELVDRRMADSGNTAGLKWTVTKAKVKKNIDTKASKGRKLRYTVQEKVQGFDVPRHTTT
WTDEQIDDLFASLLGQKIKIDENDNSDAEEDKPEEEPENDGLQIFG"
gene complement(<311296..>311814)
/gene="MCR1"
/locus_tag="AWJ20_4705"
/db_xref="GeneID:30036866"
mRNA complement(<311296..>311814)
/gene="MCR1"
/locus_tag="AWJ20_4705"
/product="Mcr1p"
/transcript_id="XM_018881794.1"
/db_xref="GeneID:30036866"
CDS complement(311296..311814)
/gene="MCR1"
/locus_tag="AWJ20_4705"
/inference="similar to AA sequence:KEGG_Orthology:K00326"
/note="Mitochondrial NADH-cytochrome b5 reductase;
involved in ergosterol biosynthesis; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0031307 - integral component of
mitochondrial outer membrane [Evidence IDA] [PMID
16689936]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005758 - mitochondrial
intermembrane space [Evidence IEA]; GO_component:
GO:0005758 - mitochondrial intermembrane space [Evidence
IDA] [PMID 8001120]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IEA,IEA];
GO_component: GO:0005741 - mitochondrial outer membrane
[Evidence IDA] [PMID 16407407]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
8001120]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0004128 -
cytochrome-b5 reductase activity, acting on NAD(P)H
[Evidence IEA]; GO_function: GO:0004128 - cytochrome-b5
reductase activity, acting on NAD(P)H [Evidence IDA] [PMID
8001120]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_process: GO:0034599 -
cellular response to oxidative stress [Evidence IMP] [PMID
11420140]; GO_process: GO:0006696 - ergosterol
biosynthetic process [Evidence IDA] [PMID 10622712];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]"
/codon_start=1
/product="Mcr1p"
/protein_id="XP_018736237.1"
/db_xref="GeneID:30036866"
/translation="MSTHIHGLKPNDVLSFKGPLPKYQWTPNAHKEIALLGGGTGITP
LYQLLQAIHKDPNDKTKVNLFYANVTKEDVLIRDEIEALIKDRPDQFKVTYFLDKPPS
GWTGETGYISKEFLSKNLPKPDSENIKIFVCGPPPFYNALSGPKVSPQDQGELTGALK
ELGYNKDQVYKF"
gene <314138..>314962
/locus_tag="AWJ20_4706"
/db_xref="GeneID:30036867"
mRNA <314138..>314962
/locus_tag="AWJ20_4706"
/product="NADHX dehydratase"
/transcript_id="XM_018881795.1"
/db_xref="GeneID:30036867"
CDS 314138..314962
/locus_tag="AWJ20_4706"
/inference="similar to AA sequence:KEGG_Orthology:K17757"
/note="NADHX dehydratase; converts (S)-NADHX to NADH in an
ATP-dependent manner; homologous to Carkd in mammals, and
the C-terminal domain of YjeF in E.coli; enzyme is
widespread in eukaryotes, prokaryotes and archaea; YKL151C
promoter contains STREs (stress response elements) and
expression is induced by heat shock or methyl
methanesulfonate; green fluorescent protein (GFP)-fusion
protein localizes to the cytoplasm; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0047453 -
ATP-dependent NAD(P)H-hydrate dehydratase activity
[Evidence IEA,IEA]; GO_function: GO:0047453 -
ATP-dependent NAD(P)H-hydrate dehydratase activity
[Evidence IDA] [PMID 21994945]; GO_function: GO:0016829 -
lyase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0046496
- nicotinamide nucleotide metabolic process [Evidence
IEA]; GO_process: GO:0046496 - nicotinamide nucleotide
metabolic process [Evidence IDA] [PMID 21994945]"
/codon_start=1
/product="NADHX dehydratase"
/protein_id="XP_018736238.1"
/db_xref="GeneID:30036867"
/translation="MASTLVGADMSHVICEKYAGDVIKSYSPNLIVHPYLFETEHLPS
GHSIQNVVEKVCSVLDRIHVVVIGPGLGRDKTMLETTAAIIQEAKKRKLPIVIDADGL
YLIQNQPDLIKGYEKAILTPNVVEFQRLCKALEIDDAGGDDPASAPKVSKLATTFGGL
TILEKGKLDNISNGHTTISNDISGGPKRVSGQGDTLSGSIAAFLAWQLAYEQKLWDTE
PIKEAQDSRDLTLLAVFAASSITRTASALAYKNHGRALLTSDISDSVGAAFKQLFE"
gene complement(<315195..>315932)
/locus_tag="AWJ20_4707"
/db_xref="GeneID:30036868"
mRNA complement(<315195..>315932)
/locus_tag="AWJ20_4707"
/product="hypothetical protein"
/transcript_id="XM_018881796.1"
/db_xref="GeneID:30036868"
CDS complement(315195..315932)
/locus_tag="AWJ20_4707"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736239.1"
/db_xref="GeneID:30036868"
/translation="MSARQLSDIEKKSPQIRSRSDELWKLFITRDFPDRPVSESHNRR
AYAQYYKEKEAHLKNASSRLRESLEKIKQEKASRTITSLEVDPQSLRAAARRKASSSA
PAGSRLIQKAFNSARTKGPLFSSRNTKFASMGMGSINGLPPGIPGAIRAPRSIPPPAM
RNVPTPSPLSMPLNARVRPTGPVSPLKPAVATKPHPTKQTAQESAIINSPDNRKKPKI
DTPPTTPTIAPATRKRPNSSIFLQRRK"
gene complement(<316979..>317716)
/gene="CBF1"
/locus_tag="AWJ20_4708"
/db_xref="GeneID:30036869"
mRNA complement(<316979..>317716)
/gene="CBF1"
/locus_tag="AWJ20_4708"
/product="Cbf1p"
/transcript_id="XM_018881797.1"
/db_xref="GeneID:30036869"
CDS complement(316979..317716)
/gene="CBF1"
/locus_tag="AWJ20_4708"
/inference="similar to AA sequence:KEGG_Orthology:K09104"
/note="Basic helix-loop-helix (bHLH) protein; forms
homodimer to bind E-box consensus sequence CACGTG present
at MET gene promoters and centromere DNA element I (CDEI);
affects nucleosome positioning at this motif; associates
with other transcription factors such as Met4p and Isw1p
to mediate transcriptional activation or repression;
associates with kinetochore proteins, required for
chromosome segregation; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0005694 - chromosome [Evidence IEA]; GO_component:
GO:0000775 - chromosome, centromeric region [Evidence
IEA,IEA]; GO_component: GO:0000775 - chromosome,
centromeric region [Evidence IDA] [PMID 9407032];
GO_component: GO:0000776 - kinetochore [Evidence IPI]
[PMID 11070082]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 11914276]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0001102 - RNA
polymerase II activating transcription factor binding
[Evidence IMP] [PMID 20935143]; GO_function: GO:0001102 -
RNA polymerase II activating transcription factor binding
[Evidence IPI] [PMID 8665859]; GO_function: GO:0000978 -
RNA polymerase II core promoter proximal region
sequence-specific DNA binding [Evidence IDA] [PMID
7891681]; GO_function: GO:0000978 - RNA polymerase II core
promoter proximal region sequence-specific DNA binding
[Evidence IDA] [PMID 9894911]; GO_function: GO:0001078 -
RNA polymerase II core promoter proximal region
sequence-specific DNA binding transcription factor
activity involved in negative regulation of transcription
[Evidence IDA] [PMID 15302821]; GO_function: GO:0001077 -
RNA polymerase II core promoter proximal region
sequence-specific DNA binding transcription factor
activity involved in positive regulation of transcription
[Evidence IDA] [PMID 7891681]; GO_function: GO:0001103 -
RNA polymerase II repressing transcription factor binding
[Evidence IDA] [PMID 12820973]; GO_function: GO:0001076 -
RNA polymerase II transcription factor binding
transcription factor activity [Evidence IMP] [PMID
12820973]; GO_function: GO:0001076 - RNA polymerase II
transcription factor binding transcription factor activity
[Evidence IMP] [PMID 20935143]; GO_function: GO:0019237 -
centromeric DNA binding [Evidence IDA] [PMID 11222754];
GO_function: GO:0019237 - centromeric DNA binding
[Evidence IDA] [PMID 2057354]; GO_function: GO:0019237 -
centromeric DNA binding [Evidence IDA] [PMID 2249662];
GO_function: GO:0019237 - centromeric DNA binding
[Evidence IDA] [PMID 9407032]; GO_function: GO:0046983 -
protein dimerization activity [Evidence IEA]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 19111667]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IDA] [PMID
19158363]; GO_process: GO:0006338 - chromatin remodeling
[Evidence IDA] [PMID 15111622]; GO_process: GO:0006338 -
chromatin remodeling [Evidence IMP] [PMID 21131275];
GO_process: GO:0006338 - chromatin remodeling [Evidence
IMP] [PMID 2249662]; GO_process: GO:0006338 - chromatin
remodeling [Evidence IMP] [PMID 7891681]; GO_process:
GO:0007059 - chromosome segregation [Evidence IMP] [PMID
2057354]; GO_process: GO:0007059 - chromosome segregation
[Evidence IMP] [PMID 2185892]; GO_process: GO:0007059 -
chromosome segregation [Evidence IMP] [PMID 2188087];
GO_process: GO:0007059 - chromosome segregation [Evidence
IGI] [PMID 9584087]; GO_process: GO:0061427 - negative
regulation of ceramide biosynthetic process by negative
regulation of transcription from RNA Polymerase II
promoter [Evidence IMP] [PMID 15302821]; GO_process:
GO:0000122 - negative regulation of transcription from RNA
polymerase II promoter [Evidence IMP] [PMID 10921921];
GO_process: GO:0000122 - negative regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 22696683]; GO_process: GO:1900375 - positive
regulation of inositol biosynthetic process by positive
regulation of transcription from RNA polymerase II
promoter [Evidence IMP] [PMID 17351075]; GO_process:
GO:1900375 - positive regulation of inositol biosynthetic
process by positive regulation of transcription from RNA
polymerase II promoter [Evidence IMP] [PMID 20935143];
GO_process: GO:1900478 - positive regulation of sulfate
assimilation by positive regulation of transcription from
RNA polymerase II promoter [Evidence IMP] [PMID 7601277];
GO_process: GO:1900478 - positive regulation of sulfate
assimilation by positive regulation of transcription from
RNA polymerase II promoter [Evidence IMP] [PMID 7891681];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 22696683]; GO_process: GO:0061432 - regulation
of transcription from RNA polymerase II promoter in
response to methionine [Evidence IMP] [PMID 7891681];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Cbf1p"
/protein_id="XP_018736240.1"
/db_xref="GeneID:30036869"
/translation="MDKPPSKRARIEDPSSANIDDALLKNDQQTAASEAVVPTTEAQQ
GETHDASNELENKMATALAAVAAAASANGGEYPSVVSRQAQTSASPYDHSRPNGSASA
GVDGHEPTISVGDSSFKPSAGSEEWHKIRRDNHKEVERRRREAINKGISELATIVPDC
EKHKGQILQKTVDYIKKLKDNEQRNMEKLTLEKLLTEQAISELSATNKALKQELAQAW
KEVEHWKQACGASTGADASKSADAGSK"
gene <318793..>318864
/locus_tag="AWJ20_4709"
/db_xref="GeneID:30036870"
tRNA <318793..>318864
/locus_tag="AWJ20_4709"
/product="tRNA-Asp"
/db_xref="GeneID:30036870"
gene complement(<319055..>321037)
/gene="BRN1"
/locus_tag="AWJ20_4710"
/db_xref="GeneID:30036872"
mRNA complement(<319055..>321037)
/gene="BRN1"
/locus_tag="AWJ20_4710"
/product="Brn1p"
/transcript_id="XM_018881799.1"
/db_xref="GeneID:30036872"
CDS complement(319055..321037)
/gene="BRN1"
/locus_tag="AWJ20_4710"
/inference="similar to AA sequence:KEGG_Orthology:K06676"
/note="Subunit of the condensin complex; required for
chromosome condensation and for clustering of tRNA genes
at the nucleolus; may influence multiple aspects of
chromosome transmission; GO_component: GO:0005694 -
chromosome [Evidence IEA,IEA]; GO_component: GO:0000796 -
condensin complex [Evidence IEA]; GO_component: GO:0005737
- cytoplasm [Evidence IEA,IEA]; GO_component: GO:0000799 -
nuclear condensin complex [Evidence IPI,ISS] [PMID
10749931]; GO_component: GO:0000799 - nuclear condensin
complex [Evidence IDA] [PMID 10811823]; GO_component:
GO:0000799 - nuclear condensin complex [Evidence IPI,ISS]
[PMID 7698648]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 10749931]; GO_function: GO:0003682 -
chromatin binding [Evidence IGI] [PMID 11864994];
GO_process: GO:0006268 - DNA unwinding involved in DNA
replication [Evidence IMP] [PMID 24062159]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0051301 - cell division [Evidence IEA]; GO_process:
GO:0030261 - chromosome condensation [Evidence IEA];
GO_process: GO:0007076 - mitotic chromosome condensation
[Evidence IEA]; GO_process: GO:0007076 - mitotic
chromosome condensation [Evidence IGI,IMP] [PMID
10749931]; GO_process: GO:0007076 - mitotic chromosome
condensation [Evidence IMP] [PMID 10811823]; GO_process:
GO:0007076 - mitotic chromosome condensation [Evidence
IMP] [PMID 7698648]; GO_process: GO:0007067 - mitotic
nuclear division [Evidence IEA]; GO_process: GO:0000070 -
mitotic sister chromatid segregation [Evidence IMP] [PMID
10749931]; GO_process: GO:0070058 - tRNA gene clustering
[Evidence IMP] [PMID 18708579]"
/codon_start=1
/product="Brn1p"
/protein_id="XP_018736241.1"
/db_xref="GeneID:30036872"
/translation="MSLLRDGDGINFQRASYTLDGCVKIYTSRIDSVATETGKLLSGL
ADAKKNRGKGGDDEEVEGDDEGHEDEDGNDEESSKKRKRRPRAENTLVEEYSQLEVKK
LDLELNIDPLFRKMCADFDEGGAKGLLLNSLMIDKTGRVVFDGDANDGDDDEDEEADK
DEVDANTSTSSSGSSKDKTLNPHFQGDTIDMDLLRSKYFSNLDDLDELEICPSLPGIY
DALNDPALLNSQLVKELQEMSIKDRADYRPFAESLDDMALGEDGYGDAPPDMDYDDMP
ELIANDDDEASFAVGKGSTTVIPYTGATSVNVMSGRDTDILSYFDETLKKNWAGPEHW
KIQKLKDTTVAGIKTETSKEIKKPKKTKEQLVIDFLSDEGAIDESELFASGGNINLPK
SQRKSKDYNLLPDDRQFSSKKLIKLFSKPKGNVISNKLFGGAITGNQTSNDSEGIQEN
DENFWAKQYQQPEDGKSGSDYNQNFFNDDDYDGMGIPDIGGDEDDGLPLDAGESLYRD
GPTQLLAKGQRARPDYVNYAKFAKRVNVKLLKDNIWSVMKLPEPSSDETANEPKIDEV
DDDDVDPSTGKPLRKPSLAPAAEFTERKFTDLAKELKQVYPAQQMSEISTSFCFICVL
HLANERGLIIEDNEMHNDLTIKWDPSVPRGTAINSF"
gene <321891..>323192
/gene="IME4"
/locus_tag="AWJ20_4711"
/db_xref="GeneID:30036873"
mRNA <321891..>323192
/gene="IME4"
/locus_tag="AWJ20_4711"
/product="mRNA (N6-adenosine)-methyltransferase"
/transcript_id="XM_018881800.1"
/db_xref="GeneID:30036873"
CDS 321891..323192
/gene="IME4"
/locus_tag="AWJ20_4711"
/codon_start=1
/product="mRNA (N6-adenosine)-methyltransferase"
/protein_id="XP_018736242.1"
/db_xref="GeneID:30036873"
/translation="MSSSSSSTIFSLNQALLYSPNTTINLSSYQNSIETPSSDIGSRI
ASHHLRSVSVTGSLDGEMSVNDNSLEEIVVSFLINHKPKLFPALPFGINVLLRTINQV
HNCHISSDAFDNACLNLAMQIPSSFVVSKMCSQNASYSSKYQLEFYDPEMLILWSSSS
LSTIFTRSSASSSGSSTLDCSISVIADHDTPNRNLNENIAYADNSKLYQTMLSLITND
DSYYAQMRQTLANLLSEPTQRNILYKEQILSASSTKPYVEICPYPKRNECLLQGRLDC
LRKIHFEPVITSNTDVSIGDCSYLDTCFKGKGCKYVHYRITYPDTFQRGQARQPQEAK
QNQGPLFHIAERDYSKPKVGQNISEKYLVLITRQIHKCLLTGLFRLLPSGFAAISGDL
TSAFSVTLVSSLPTVSAIEPQTTDLDNQFNLTDFQLLGIYI"
gene <324452..>327079
/gene="CUL3"
/locus_tag="AWJ20_4712"
/db_xref="GeneID:30036874"
mRNA <324452..>327079
/gene="CUL3"
/locus_tag="AWJ20_4712"
/product="cullin CUL3"
/transcript_id="XM_018881801.1"
/db_xref="GeneID:30036874"
CDS 324452..327079
/gene="CUL3"
/locus_tag="AWJ20_4712"
/inference="similar to AA sequence:KEGG_Orthology:K03869"
/note="Ubiquitin-protein ligase; forms a complex with
Elc1p that polyubiquitylates monoubiquitylated RNA
polymerase II to trigger its proteolysis; cullin family
member with similarity to Cdc53p and human CUL3;
GO_component: GO:0031463 - Cul3-RING ubiquitin ligase
complex [Evidence IDA] [PMID 16675952]; GO_component:
GO:0031463 - Cul3-RING ubiquitin ligase complex [Evidence
IMP] [PMID 17296727]; GO_component: GO:0031461 -
cullin-RING ubiquitin ligase complex [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0031625 - ubiquitin
protein ligase binding [Evidence IEA]; GO_function:
GO:0004842 - ubiquitin-protein transferase activity
[Evidence IDA] [PMID 12676951]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence IDA]
[PMID 16675952]; GO_process: GO:0016567 - protein
ubiquitination [Evidence IEA]; GO_process: GO:0042787 -
protein ubiquitination involved in ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 17296727];
GO_process: GO:0006511 - ubiquitin-dependent protein
catabolic process [Evidence IEA]; GO_process: GO:0006511 -
ubiquitin-dependent protein catabolic process [Evidence
IC] [PMID 12676951]; GO_process: GO:0006511 -
ubiquitin-dependent protein catabolic process [Evidence
ISS] [PMID 9531531]"
/codon_start=1
/product="cullin CUL3"
/protein_id="XP_018736243.1"
/db_xref="GeneID:30036874"
/translation="MATRGGRARIRAPKKSLADEANFDVSWNEKLSPAIREIYDKNAS
KRSFEELYRTAYYLVLKKHGKRLYESTKQQIREHLENKVVGSLTRYIQDHGQNSGSGT
SVAAGGDDNVDGTIRENGTELATTENATKTTPKGTLDLESVPVDFLKQVRRYWDDSCL
CMKMTSDILMYLDRVYVQDAKVPLIYDAGLILFRDAVIFNQKLPVGSIIDNIIINEVI
NERNGQIIDRVAIKATVNMLESLRDSHNPLFTSPIRTAQNDGRPYDYLKNGTDGLIDL
DSGFGSVYTGEFEPKFLASSEEYYSTTARNVLESTPDAHVYIQKVKFWLEEEAQRCNR
YLSIHTLDKVITLIEDVLITQRISEVLELPTTGLVSWIENEQYEDLSLAYQLVGRVDK
TYKCICELVKKDLLIAGREANNVAIRSSEEAKASKGRPKEKQDAANPASGAAGAGATA
TLNPTTIAINWVEQVIRLKDKYDKILIQCFENQQNIRIANEESFILFVNENPKVAEFL
SLFIDENLKRTIKGKSDEEIEDTLEKAITLFRYIADKDLFETYYKVHLAKRLLNGKSV
SDEVERNMIGKIKREVGTAFTSKLEGMFKDMRISKDINNEFKAAQSQLQQSDSGNKGL
ADIQVNVLTSTFWPSGIVNTQAKFVLPPVVDEAKQRFEKFYLGRHTGRVLAWNMSMGT
ADIKARFKKRTHEINLSTVAMVVLMLFNELPEGHYLTYEDIQNTTGIATSELTRHLQS
IAVAPRTRLLRKEPMSRDVKPTDKFYFNDKFESPMARIKVLAVSAKSKAENDSERKET
LDRVDQSRKYETDAAIVRIMKSRRTLEHVQLIADATRQLSSRFKPDPILIKQRIDALL
EREYLERDPERRNVYNYLA"
gene complement(<327291..>328283)
/locus_tag="AWJ20_4713"
/db_xref="GeneID:30036875"
mRNA complement(<327291..>328283)
/locus_tag="AWJ20_4713"
/product="hypothetical protein"
/transcript_id="XM_018881802.1"
/db_xref="GeneID:30036875"
CDS complement(327291..328283)
/locus_tag="AWJ20_4713"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736244.1"
/db_xref="GeneID:30036875"
/translation="MSSLSPIPTSAAERLIRNPHSLPRVPTALANIISQNKHSDRIKH
HAEQAYFIRRAKLENQRSERILAAATTNGTSLSGSQYRDANATPSPPSSPILDAVSPT
NSRPRVFTHRRNSDTVTSTSVFSPSTQPIDIRRRSSSFTGHNGSPPRSGRPSPSRASR
SLSFSHVNNRRLSSIDPRESTTALFNSGVYFIPESPEEDELISLLYPLDATYHGPPVS
SSSGPGSPPPNLQHNMAFSSRIFSKKPEERLKQRCYFTKRPAPSWKSRNFEPIKQGKT
WVSAHPGWHRFMRNEYKVNGQEDTWREERSRFLQEFRRERQFQKQSKRRMSSHL"
gene complement(<330502..>330918)
/gene="RPS17B"
/locus_tag="AWJ20_4714"
/db_xref="GeneID:30036876"
mRNA complement(<330502..>330918)
/gene="RPS17B"
/locus_tag="AWJ20_4714"
/product="ribosomal 40S subunit protein S17B"
/transcript_id="XM_018881803.1"
/db_xref="GeneID:30036876"
CDS complement(330502..330918)
/gene="RPS17B"
/locus_tag="AWJ20_4714"
/inference="similar to AA sequence:KEGG_Orthology:K02962"
/note="Ribosomal protein 51 (rp51) of the small (40s)
subunit; homologous to mammalian ribosomal protein S17, no
bacterial homolog; RPS17B has a paralog, RPS17A, that
arose from the whole genome duplication; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0022627 - cytosolic small ribosomal
subunit [Evidence IBA]; GO_component: GO:0022627 -
cytosolic small ribosomal subunit [Evidence NAS] [PMID
9559554]; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA,IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IEA]; GO_function: GO:0003735 - structural constituent of
ribosome [Evidence NAS] [PMID 9559554]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence NAS] [PMID
9559554]; GO_process: GO:0000028 - ribosomal small subunit
assembly [Evidence IMP] [PMID 3915776]; GO_process:
GO:0006412 - translation [Evidence IEA]; GO_process:
GO:0006414 - translational elongation [Evidence IBA]"
/codon_start=1
/product="ribosomal 40S subunit protein S17B"
/protein_id="XP_018736245.1"
/db_xref="GeneID:30036876"
/translation="MGRVRTKTVKRAAKQIIEGYYPKLTLDFETNKRLADEIAFIPSK
RLRNKIAGYTTHLMKRIQKGPVRGISFKLQEEERERRDQYVPEVSALDVSQTENGLEV
DADTANLLKTLGFAKLPVSVVHVSQQAPERRFRKRN"
gene <332839..>334332
/gene="ADA2"
/locus_tag="AWJ20_4715"
/db_xref="GeneID:30036877"
mRNA <332839..>334332
/gene="ADA2"
/locus_tag="AWJ20_4715"
/product="chromatin-binding transcription regulator ADA2"
/transcript_id="XM_018881804.1"
/db_xref="GeneID:30036877"
CDS 332839..334332
/gene="ADA2"
/locus_tag="AWJ20_4715"
/inference="similar to AA sequence:KEGG_Orthology:K11314"
/codon_start=1
/product="chromatin-binding transcription regulator ADA2"
/protein_id="XP_018736246.1"
/db_xref="GeneID:30036877"
/translation="MIVEQHAYPIFDDDWGADEELLLLEGAELQGLGNWQDISDHIGG
RSREEVDEHYNKVYLQSATYPLPEMNKKFNISPAEFAEKKRQRLEDRRKNAPALPIPK
QKPTASVPACHEVQGYMPGRLEFEQETENEAEMSVKDMVFDPEEPSQEVNFKLTILDI
YNSRLTTRAERKRSILAHGLLDYRKNAGIEKKRTKEERDLVNKLKPFARMMTRKDYEM
LTDSMLTELRCRRRIAELQEYRRNGIRTLEAAAKYERDKVTRINALNRAGIILGGGMT
PSSNSTILNNSNIINGTSLTRNSLGSKPGTPGPLNSTSSIDSTSTVNDSTSSTNGSSN
VNNTAVSVMSPLGYKFSPSVFTNISESERSSPFGSLSLPGTSSSTALNGGSGSGLGST
GGSVTPTATTNGTTLKIRKSISMTTPPLDISQAADVELLSPQEQLLCSQLRILPKPYL
AIKETLFRELVRTGGILKRKTARELIKIDAAKTGRIYEFFQSQNWLS"
gene complement(<334548..>335276)
/locus_tag="AWJ20_4716"
/db_xref="GeneID:30036878"
mRNA complement(<334548..>335276)
/locus_tag="AWJ20_4716"
/product="hypothetical protein"
/transcript_id="XM_018881805.1"
/db_xref="GeneID:30036878"
CDS complement(334548..335276)
/locus_tag="AWJ20_4716"
/inference="similar to AA sequence:KEGG_Orthology:K14397"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736247.1"
/db_xref="GeneID:30036878"
/translation="MTTVTETARKTTILPPLPKPFTEKQAPTIRLYPVSNYTFDVKEA
QPEEDNSVTGRLQRLQEQYETSGMRRTCEGILVCHEHGHPYVLLLQIANTFFKLPGDY
IQPDQDEITGFKQRLNERLAPAKDSEHASDPINSEWEIGDCITQWWRPNFETFMYPFI
PPHISRPKECKKMFLIHLPKHKVLSVPQNMKLLAVPLFELYDNATRYGNQLAAIPLYL
SRYNFEFVDSDDNVIATYPASNAN"
gene complement(<337729..>338937)
/gene="UTP6"
/locus_tag="AWJ20_4717"
/db_xref="GeneID:30036879"
mRNA complement(<337729..>338937)
/gene="UTP6"
/locus_tag="AWJ20_4717"
/product="Utp6p"
/transcript_id="XM_018881806.1"
/db_xref="GeneID:30036879"
CDS complement(337729..338937)
/gene="UTP6"
/locus_tag="AWJ20_4717"
/inference="similar to AA sequence:KEGG_Orthology:K14557"
/note="Nucleolar protein; component of the small subunit
(SSU) processome containing the U3 snoRNA that is involved
in processing of pre-18S rRNA; GO_component: GO:0030686 -
90S preribosome [Evidence IDA] [PMID 12150911];
GO_component: GO:0034388 - Pwp2p-containing subcomplex of
90S preribosome [Evidence IDA] [PMID 15231838];
GO_component: GO:0034388 - Pwp2p-containing subcomplex of
90S preribosome [Evidence IDA] [PMID 17515605];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0005730 - nucleolus [Evidence IEA];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
12068309]; GO_component: GO:0005730 - nucleolus [Evidence
IDA] [PMID 12150911]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0032040 - small-subunit processome [Evidence IDA] [PMID
12068309]; GO_function: GO:0030515 - snoRNA binding
[Evidence IPI] [PMID 12068309]; GO_process: GO:0006396 -
RNA processing [Evidence IEA]; GO_process: GO:0000480 -
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 15489292]; GO_process: GO:0000447 - endonucleolytic
cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and
LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000472 - endonucleolytic cleavage to
generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000462 - maturation of SSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 12068309]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA]; GO_process:
GO:0042254 - ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Utp6p"
/protein_id="XP_018736248.1"
/db_xref="GeneID:30036879"
/translation="MSDKVRFYLEQSVAELEDLRKKELFTKQELAVIMRKRTDHEHRI
SGRTVKPRDFLKYAEYEINVERLRKKRVKRLGNKDVGGKSGISDWAGPRRIMFIFDRS
TKRFPGEIQLWLQYLEYAKQQKAIHVINKIFTTMLQLHPTKPKLWILAAKHESDENAS
MKAARYILQRSLRFNYDSELLWYEYIKLELIYVSKILARRKLLGLDLDTEEQQAQKEQ
DEEDEESEAHIELPDIKEQVKDDLKALPEADMSMLGNPQTNPALRGDVALAIYDASME
RAKTVDAKYDFSLKIIELFDSFIDLDRAHLSKHVIDYLSHTQASPKTLFLSTTLPVRY
VSHDDPTFPDMIKLMITKYNKSLSLEEKTPELKQHLREFLTSKYLEPTLEPALDQNIR
LVLQSFTKKL"
gene <339974..>341797
/gene="AMF1"
/locus_tag="AWJ20_4718"
/db_xref="GeneID:30036880"
mRNA <339974..>341797
/gene="AMF1"
/locus_tag="AWJ20_4718"
/product="Amf1p"
/transcript_id="XM_018881807.1"
/db_xref="GeneID:30036880"
CDS 339974..341797
/gene="AMF1"
/locus_tag="AWJ20_4718"
/note="Putative paralog of ATR1; but not required for
boron tolerance; member of the DHA2 family of drug:H+
antiporters; YOR378W is not an essential gene;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0008150 - biological_process
[Evidence ND]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Amf1p"
/protein_id="XP_018736249.1"
/db_xref="GeneID:30036880"
/translation="MPDNEQNQNGDSFHEDRPVVTNRPSHTSAIPTSNPPSSPPSIRH
VESETNQLWSRMSIKMSVVEQQNSGLNSEIASENHVDLYHQGEAEETDLDVASRVPTN
MSALSELAMKRPACFRSTFHEVVCVFILTMAPVLSSTNNGVLYIALPRIGKEFRVADG
TLSWTMSASSLATGAFLLLMGQLADTIGRRRMVLFSYAFYTLMSLGAGLCKNYIGFTI
LRALQGIASAAGVTAAVGILGSEYLPGKRRNFALAAFSSGAPLGFVFGLLAGGISTEI
LNWESSLYFFAMVYFVLTIIGFFVIPKSDPRFDRRFLYESLQKIDYVGALLTISGFTL
FVFALSQWSSTAHGWKTPYILVCLFLGALIIALLCVYEGYVPARPLMPMHIWIAPGFA
LCMATIICGWILFTGVVSYYATLYFQNIRHTSPILTTACFLPMAIGGIMVNVFAGLVL
HKISGRILLIVAMASFTISALLWSFVGEHTLYWALPFPGLLLVVVGADLAYNVCNMHA
LSTVDKSLQSTAAGVFNTCLQLATALGLAISSSVVAAVVPDQLNATPSELLKGYRAGY
WFATGVGGLGLIFSFFLKIGTQGGRTKRKTVSEKDPAPSDV"
gene complement(<343594..>346173)
/gene="YPK1"
/locus_tag="AWJ20_4719"
/db_xref="GeneID:30036881"
mRNA complement(join(<343594..345933,346144..>346173))
/gene="YPK1"
/locus_tag="AWJ20_4719"
/product="serine/threonine protein kinase YPK1"
/transcript_id="XM_018881808.1"
/db_xref="GeneID:30036881"
CDS complement(join(343594..345933,346144..346173))
/gene="YPK1"
/locus_tag="AWJ20_4719"
/inference="similar to AA sequence:KEGG_Orthology:K13303"
/codon_start=1
/product="serine/threonine protein kinase YPK1"
/protein_id="XP_018736250.1"
/db_xref="GeneID:30036881"
/translation="MDQTSQESSFRSTTGSGAAPQPPEAAPEPKLTNPLELKETHLSA
AFSPRSSASSSSTNGGADSSPNGKSGASSGASSTSDLAGSNNSNRTPVSFVSVTTGHH
SPGRKIPNSSASSLETISGNNSSGAATSGSPSVVPALSSSVSSSVSLNANSTNSSSGQ
AANPAISSASSSSSAAAVASASAAAADAAIKEGDPATSTSTITPGLLSVSITKAVGLS
LPPGNVWPNYILNATNSSIGAGSVSGSIAPGGTGSGIGGVGSLSNGNSISPRKQQLSS
PSQLHQLQRDKQSPSNLGRRRPPPIYIVLEYDKTQVILDAMGGTVDGPIWDKVSNFDV
SRIESLSIQVFAKIPVTVNTDGTSSVGAGGSVIVGGESPFTPPRQSLGDNGTAESTRG
GNLNSEALRKKGYQEIFLGTCKIDANLSKTVICDGWLPLSSGTGKIQVRIEFKANKNN
KHLTIEDFDLLKVVGKGSFGKVMQVRKIDTRRIYALKTIRKAHIISRSEVNHTLAERT
VLAQIDNPFIVPLKFSFQSPEKLYLVLAFVNGGELFHHLQREGRFDLNRSRFYTAELL
CALECLHEYNVIYRDLKPENILLDYTGHIALCDFGLCKLNMAGQEKTNTFCGTPEYLA
PELLLGQGYTKVVDWWTLGVLFYEMLTGLPPFYDQDTNEMYRKILQDPLRFPDDMDRE
ARSLLTGLLNRDPKKRLGVNGAAEIKAHPFFAQIDWKRLMNKKYAAPFKPSVESATDT
SNFDQEFTSEVPTDSVVEKDFYLSESVQQQFGGWTYTNTDSKLASSGRW"
gene <352921..>356430
/gene="HSL1"
/locus_tag="AWJ20_4720"
/db_xref="GeneID:30036883"
mRNA <352921..>356430
/gene="HSL1"
/locus_tag="AWJ20_4720"
/product="protein kinase HSL1"
/transcript_id="XM_018881810.1"
/db_xref="GeneID:30036883"
CDS 352921..356430
/gene="HSL1"
/locus_tag="AWJ20_4720"
/inference="similar to AA sequence:KEGG_Orthology:K02515"
/codon_start=1
/product="protein kinase HSL1"
/protein_id="XP_018736251.1"
/db_xref="GeneID:30036883"
/translation="MTTVAVAAAQAATHTASSGPAAGSDEAVKANHRLSQTSNHSSAS
KRSKSHVGPWRLGRTLGRGSSGRVRLAKHSLTGQLAAVKIVPKEIVVGGGSAGNGAAT
SPSANGGAGPGATAVVDATQYGSDPKDQGGLPYGIEREVIIMKLIEHPNVMALYDVWE
NKGELYLVLEYVEGGELFDYLIKKGRLDEREAVNYFRQIIYGVDYCHKFNICHRDLKP
ENLLLDKNRNIKIADFGMAALETSDRMLETSCGSPHYASPEIVAGKNYHGAPSDIWSC
GVILFALLTGHLPFDDDNIRKLLLKVQTGKFIMPSDISSEAQDLIWRMLRVDPIKRIS
MAEILRHPLLKKYPVRKNGHHNTRLHQPVDTIFANADRPVKTVSDIDPEILKNLQILW
HGASKKAVIEKLLAPGPNSEKTFYCLLMKYRHDHRQDEEYSNNKNNSVSRDNESSSHH
HASRHNHTHTHHQSHHRKRSSQVSITLKKTAVRNAAGAAGPHSRNHSRSNSVASVITA
SSSHRRNVAFVRKRASSSHMRHQSGNTTPQKTTPPSSIIARNATTTTTTSTANPTSAG
ATTTSTEIETATTAVTAKPQVESLPIPRTYINQTPTPHLTPEEDFNFISKRASVELAS
MCEQAFFSTDLPSRTSDRAVSDPLSSIKAPKMATTEKRVFSLGEHEVNSVEPGSRPYS
AYSTVSEASTTSTATPYFLPMIFEEDRFADAIEEEVDLKIYKRPNRAHDKRDTITQDY
DYDYDYFKQESVLAQPKKRELVPPSAHTAVTKESSRLQISGLVKTDSFKARSHTGFAA
YRTPSAPVPKPNPVVTTEPVLLISPSPPPSPIEATNSITTATGKDSSDNTTKPSQSDR
RPNTANNNTKSSAKSTNRPATTTKQAPVVSTSTITTTNASKPISNPYKNSQVSQVQRT
KTDPLKNHDQQPVSKPVRKPLAVKSNNIMITSNDVTDMSDLKSKEAAATAKPNAATSL
FRRLTLNPRRPPPPPPPATAVSTTQPQAVRPAPPPPPAPTVATRTSSGKSNEVKQNWF
MKMLNPNPQYTTRPTPSTAKKSQYATKTLFSTSSVTTMRHIIVEVLEDWQKYGISQIN
EDSVSSTVTAVISSRNVLSMRSAKFRINVYPMQGGTRAVFTQEKGSNTTFLRFLGELE
RALGEMDVLSPPRTDARTAIGLR"
gene <356877..>358460
/gene="NAM9"
/locus_tag="AWJ20_4721"
/db_xref="GeneID:30036884"
mRNA <356877..>358460
/gene="NAM9"
/locus_tag="AWJ20_4721"
/product="mitochondrial 37S ribosomal protein NAM9"
/transcript_id="XM_018881811.1"
/db_xref="GeneID:30036884"
CDS 356877..358460
/gene="NAM9"
/locus_tag="AWJ20_4721"
/note="Mitochondrial ribosomal component of the small
subunit; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005763 - mitochondrial
small ribosomal subunit [Evidence IPI] [PMID 11278769];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IEA,IEA]; GO_function:
GO:0019843 - rRNA binding [Evidence IEA,IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IPI] [PMID 11278769]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IC] [PMID 11278769]"
/codon_start=1
/product="mitochondrial 37S ribosomal protein NAM9"
/protein_id="XP_018736252.1"
/db_xref="GeneID:30036884"
/translation="MTRRNKVLFSLTRKRIRPSYNKYNVFNAMTWRKNIRRGTLYQQI
FSAKQESRSYHGEHLTETQFRNIFKSELNSVNPLQSTKSAGSAESKSSQDEIPWALQT
YVGLERRLDTAVFRAMFASSIRQSAQFIVRGNVKVNGIKIKQPGYILRPGDVFSVEPE
RVLEALGRQKPTIKQSVELVNKQIKKYNKYLDKCRAKPEVMWRIRQHNRKRNKAYYTK
YLEKQTARNIAWNKQVREDMSKKIDEVTPVSVLEGILRNEAYFNKHGTLPQDGGKAIL
AKSLSIYGLVKGVNVNLKVDSAPAVKAAATETTTENTASETASETASETATETATEST
TESSAAAAAETGEVASADVSKTVSKYYSKEESGESGPANKSDVKKLVQDVVKLRQEHI
RQTYSSKIRDLKNSSEEYDPTWVDRLPEKLPIVRAKDAREDINSVLPIRLPYGHGKLY
GLSKPEKGYFTPWTPRQFLTPFAVLPHHIEVSFLTCHAVYLRDPVARPGHSELVSPFP
LDMHERAHMYYFARRRKNI"
gene complement(<358683..>360675)
/gene="SRV2"
/locus_tag="AWJ20_4722"
/db_xref="GeneID:30036885"
mRNA complement(join(<358683..360369,360614..>360675))
/gene="SRV2"
/locus_tag="AWJ20_4722"
/product="Srv2p"
/transcript_id="XM_018881812.1"
/db_xref="GeneID:30036885"
CDS complement(join(358683..360369,360614..360675))
/gene="SRV2"
/locus_tag="AWJ20_4722"
/inference="similar to AA sequence:KEGG_Orthology:K17261"
/note="CAP (cyclase-associated protein); N-terminus binds
adenylate cyclase and facilitates activation by RAS;
N-terminus forms novel hexameric star-shaped shuriken
structures that directly catalyze cofilin-mediated
severing of actin filaments; C-terminus, in physically and
genetically separate activity, binds and recycles cofilin
bound, ADP-actin monomers, facilitating regulation of
actin dynamics and cell morphogenesis; GO_component:
GO:0030479 - actin cortical patch [Evidence IEA];
GO_component: GO:0030479 - actin cortical patch [Evidence
IDA] [PMID 8552082]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005856 - cytoskeleton
[Evidence IEA]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 19053807];
GO_function: GO:0003779 - actin binding [Evidence
IEA,IEA]; GO_function: GO:0003779 - actin binding
[Evidence IDA] [PMID 19201756]; GO_function: GO:0008179 -
adenylate cyclase binding [Evidence IDA] [PMID 2184942];
GO_process: GO:0007265 - Ras protein signal transduction
[Evidence IMP] [PMID 9774417]; GO_process: GO:0030036 -
actin cytoskeleton organization [Evidence IEA];
GO_process: GO:0007015 - actin filament organization
[Evidence IDA,IMP] [PMID 19201756]; GO_process: GO:0051014
- actin filament severing [Evidence IDA] [PMID 23135996];
GO_process: GO:0000902 - cell morphogenesis [Evidence
IEA]; GO_process: GO:0007010 - cytoskeleton organization
[Evidence IEA]"
/codon_start=1
/product="Srv2p"
/protein_id="XP_018736253.1"
/db_xref="GeneID:30036885"
/translation="MSVNENTPSGPGYNIVTLMKRLEAATSRLEDLTVYHSEALSRSA
LGTAAGVTAEKRAEAAAGSDAGSAVGEKGVGSGADSIATSPTDTSGSGGVGSTPAAKS
VDSPSSSPIIEEFETIIKTGLTQLLALSKSLDPLIEEQVIPLLRAFEAERDFLIVSLK
ASKPDVSSKTFSELFAPIQQHAETIINIRETNRGSKFINHLATVAEGIPALGWVTVDS
PVDYISDFKDSAQFYSNRILKEYKDVEKKHVDWARTFSSVIVDLQSFVRKHYPKGLQW
NENGKPLDVVIKEKNEEGASTSVIATPAVTPPDSTSGAAKTGGAPPPPPPPPASLFTD
DAKSGPETDSSSSSGGGMNAVFAALNQGEGITSSLKKVDKSEMTHKNPALRAGANEAS
KVKKSPPPPKKPASLAAGAPSAASNVQSKKKPAHKELIDTKWIVEYFENEHKIVIEGE
MNQGVFIDRCSNCTIQIKGKVSAITINECSKVGVLFENLVSGVDVIKSTGFGLQVTGS
MPTLTIDQSHEGQIYLSEESLNVDIYTSQTSAINIEVPRNAEEGDYSETPLPEQVVHR
IGPNGQLTSTVVAIDG"
gene <361197..>363431
/gene="ARP5"
/locus_tag="AWJ20_4723"
/db_xref="GeneID:30036886"
mRNA <361197..>363431
/gene="ARP5"
/locus_tag="AWJ20_4723"
/product="Arp5p"
/transcript_id="XM_018881813.1"
/db_xref="GeneID:30036886"
CDS 361197..363431
/gene="ARP5"
/locus_tag="AWJ20_4723"
/inference="similar to AA sequence:KEGG_Orthology:K11672"
/note="Nuclear actin-related protein involved in chromatin
remodeling; component of chromatin-remodeling enzyme
complexes; promotes nucleosome shifts in the 3 prime
direction; GO_component: GO:0031011 - Ino80 complex
[Evidence IEA]; GO_component: GO:0031011 - Ino80 complex
[Evidence IDA,IMP] [PMID 12887900]; GO_component:
GO:0031011 - Ino80 complex [Evidence IDA] [PMID 15525518];
GO_component: GO:0031011 - Ino80 complex [Evidence IPI]
[PMID 24034245]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 10923024]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 11011149]; GO_function:
GO:0043140 - ATP-dependent 3'-5' DNA helicase activity
[Evidence IDA] [PMID 10952318]; GO_process: GO:0006281 -
DNA repair [Evidence IEA]; GO_process: GO:0006338 -
chromatin remodeling [Evidence IMP] [PMID 12887900];
GO_process: GO:0006338 - chromatin remodeling [Evidence
IPI] [PMID 15525518]; GO_process: GO:0042766 - nucleosome
mobilization [Evidence IDA] [PMID 12887900]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA,IEA]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Arp5p"
/protein_id="XP_018736254.1"
/db_xref="GeneID:30036886"
/translation="MPPSIDSVKVEEPVEEFAPVLYPLRDIEKPEAVEPITGGWKEGV
PIAIDFGSYQTRIGYANEESPSHEFFSQVSKFRDRRANRSYTLVGNDIYLDATARQSI
KSPFDGPLISNWDATETILDYSFAKLSVGSRNGVENPIVMTEILGAPEPQRKFMNELL
FEGYNVPSLTYGIDSLFSFHYNQGVNVSDSSHTGIVVSCGHEATHIIPVIKGRGILSQ
AKRINWGGRQASSYLQALLALKYPYFPTKVTPSQATYMIHDHCYVSSDYPQELSNYLE
YSTMSTRERIIQAPFTEVVVQEKSEEELARIAEQRKESGRRLQEQAAKKRLEKLVQQE
QELEYYNELKNRIASAPNKKEVKRMLDGEGIKDEAALQKTISELEKTIRRGRKQDVGD
DTEEQVAPSFPLVEIPDEDLDEEQIKEKRRQRLLKASYDARMRAKEEKLLEEQRLAEE
ARKDDEWREADLEGWIKEKRDKLDGLLANVREKERLREELSNRKSHASQMRMKSIAVL
ASDSRGGNKRRRAAGAGDDDPDDTFGANDDDWAVYRRIANASDSEEEEEEKNTIKALQ
EQLLQHDPTFSIEDTREAQLDWRKSTIHMFLRGPHEFDPESRAQSHQLNLNVERIRVP
EVLFQPSIAGVDQAGIVEVCGDILLRNKTIPYANNLFLHGGQAHFPNFEDRLFNELRA
ILPVNTSLSVKRASNLSFDAWRGMAQWSLTDDFRSSVVTREDYLEMGSGYIKEHGFGN
ALVR"
gene <363829..>363940
/locus_tag="AWJ20_4724"
/db_xref="GeneID:30036887"
tRNA <363829..>363940
/locus_tag="AWJ20_4724"
/product="tRNA-Leu"
/db_xref="GeneID:30036887"
gene complement(<364114..>365994)
/gene="NCL1"
/locus_tag="AWJ20_4725"
/db_xref="GeneID:30036888"
mRNA complement(<364114..>365994)
/gene="NCL1"
/locus_tag="AWJ20_4725"
/product="Ncl1p"
/transcript_id="XM_018881814.1"
/db_xref="GeneID:30036888"
CDS complement(364114..365994)
/gene="NCL1"
/locus_tag="AWJ20_4725"
/inference="similar to AA sequence:KEGG_Orthology:K14835"
/note="S-adenosyl-L-methionine-dependent tRNA:
m5C-methyltransferase; methylates cytosine to m5C at
several positions in tRNAs and intron-containing
pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C
at wobble position in response to hydrogen peroxide,
causing selective translation of mRNA from genes enriched
in TTG codon; loss of NCL1 confers hypersensitivity to
oxidative stress; similar to Nop2p and human proliferation
associated nucleolar protein p120; GO_component:
GO:0005730 - nucleolus [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9767141];
GO_function: GO:0003723 - RNA binding [Evidence IEA,IEA];
GO_function: GO:0008168 - methyltransferase activity
[Evidence IEA,IEA]; GO_function: GO:0016428 - tRNA
(cytosine-5-)-methyltransferase activity [Evidence IEA];
GO_function: GO:0016428 - tRNA
(cytosine-5-)-methyltransferase activity [Evidence IDA]
[PMID 10445884]; GO_function: GO:0000049 - tRNA binding
[Evidence IEA]; GO_function: GO:0000049 - tRNA binding
[Evidence IDA] [PMID 17567576]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0070301 - cellular response to hydrogen peroxide
[Evidence IMP] [PMID 22760636]; GO_process: GO:0032259 -
methylation [Evidence IEA]; GO_process: GO:0030488 - tRNA
methylation [Evidence IDA] [PMID 10445884]; GO_process:
GO:0008033 - tRNA processing [Evidence IEA]; GO_process:
GO:0002127 - wobble base cytosine methylation [Evidence
IMP] [PMID 22760636]"
/codon_start=1
/product="Ncl1p"
/protein_id="XP_018736255.1"
/db_xref="GeneID:30036888"
/translation="MGRKAKNKQPAPPSLDEFHEGKKRKGHKSAGSSSSSSKKHKPNG
TNGTTEHKSKRSSKSKTSAPIDKEEIEKTKKSLFENSDEEEDDDVVGSELDDDEFYDS
DEDRQAKPMFSDDDEDDEILEQMNKKAAATFGLEDSEDEEDDDEDEEENDEFDVDEED
EDDDEDDEDGEDDSGKYATEEAPKAHVLPSEQEKEIETQHPTDLHALRTRILEVVKVL
EDFKTLAEEGRSRSEYTDQLLDDIAAYYGYSRYLAEKLFNLFSPAEAIEFFEANEVAR
PVTIRTNTLKTRRRDLAQALINRGVNLQPIGKWTKVGLQVFESQVPIGATPEYLAGHY
ILQAASSFLPVMALDPQENERVLDMASAPGGKTTFISAMMKNTGCVFANDSNKSRTKS
LIANIHRLGCSNTIVCNYDAREFPKVIGGFDRVLLDAPCSGTGVIAKDPAVKTSRTEK
DFIQLPHLQKQLLLSAIDSVDASSKTGGVIVYSTCSVMVEENEAVVDYVLRKRPNVKL
VETHLEIGKEGFTNFRGKKFDPSVKLTRRYYPHTYNVDGFYVAKFQKTSGSFHDNSKA
GAREKERAIREDEDAEPLIHGDFAEFNDPEDSALIEQTRRRSMKKKGLNPNAHKPKPA
KN"
gene <366366..>367721
/gene="GCD10"
/locus_tag="AWJ20_4726"
/db_xref="GeneID:30036889"
mRNA <366366..>367721
/gene="GCD10"
/locus_tag="AWJ20_4726"
/product="Gcd10p"
/transcript_id="XM_018881815.1"
/db_xref="GeneID:30036889"
CDS 366366..367721
/gene="GCD10"
/locus_tag="AWJ20_4726"
/inference="similar to AA sequence:KEGG_Orthology:K03256"
/note="Subunit of tRNA (1-methyladenosine)
methyltransferase with Gcd14p; required for the
modification of the adenine at position 58 in tRNAs,
especially tRNAi-Met; first identified as a negative
regulator of GCN4 expression; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 9851972]; GO_component:
GO:0031515 - tRNA (m1A) methyltransferase complex
[Evidence IDA,IPI] [PMID 10779558]; GO_function:
GO:0003723 - RNA binding [Evidence IEA]; GO_function:
GO:0016429 - tRNA (adenine-N1-)-methyltransferase activity
[Evidence IDA] [PMID 10779558]; GO_function: GO:0003743 -
translation initiation factor activity [Evidence IEA];
GO_process: GO:0006446 - regulation of translational
initiation [Evidence IEA]; GO_process: GO:0030488 - tRNA
methylation [Evidence IDA] [PMID 10779558]; GO_process:
GO:0008033 - tRNA processing [Evidence IEA]; GO_process:
GO:0006413 - translational initiation [Evidence IEA]"
/codon_start=1
/product="Gcd10p"
/protein_id="XP_018736256.1"
/db_xref="GeneID:30036889"
/translation="MTSTRQQKQLQSLQQQDPESIDYSHEFDQTHIKPGQYVMIRLPS
ENFRVLQLHPGQTVNLGKFGSFKVDDILNHPYGYTYEIGADNKLSIVNHNYELDNQDE
PVSELAPHENNRELVDDSSVQGLSMAEIEELKKGGLSGKQIIEKVKTSHSSFDKKTAF
SKEKYLKRKQQKFLNQFTPEPIGSAELIDIYLDKDASRIQNMSEESLALMLSLANVRP
GGTYLVVDDISGLLVGAMMERMGGEGLIVVAHDNEHPNLDGLRYMNYSQGLIAKMIKT
VNWLEFFEPSEIEPFEEKTTEELQQLKSNQRGQYYRRRTRYNDSVTVQNLISNSGFDG
LLVATGLHLASLIPRIIPAVGGSRPIVMFSETKELLTETTLVLHKDFRVISPTILETR
LRKYQTLPGRMHPHMTSRGGGGYVLWGIRVFPDENANAVGKVHRKRKATDSGTSTPAE
E"
gene complement(<367873..>368628)
/gene="MTQ1"
/locus_tag="AWJ20_4727"
/db_xref="GeneID:30036890"
mRNA complement(<367873..>368628)
/gene="MTQ1"
/locus_tag="AWJ20_4727"
/product="Mtq1p"
/transcript_id="XM_018881816.1"
/db_xref="GeneID:30036890"
CDS complement(367873..368628)
/gene="MTQ1"
/locus_tag="AWJ20_4727"
/note="S-adenosylmethionine-dependent methyltransferase;
methylates translational release factor Mrf1p; similar to
E.coli PrmC; is not an essential gene; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14562095]; GO_function: GO:0008757 -
S-adenosylmethionine-dependent methyltransferase activity
[Evidence IDA,IMP] [PMID 16321977]; GO_function:
GO:0008168 - methyltransferase activity [Evidence
IEA,IEA]; GO_function: GO:0008276 - protein
methyltransferase activity [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0032259 - methylation [Evidence IEA];
GO_process: GO:0006479 - protein methylation [Evidence
IEA]; GO_process: GO:0006451 - translational readthrough
[Evidence IGI] [PMID 16321977]"
/codon_start=1
/product="Mtq1p"
/protein_id="XP_018736257.1"
/db_xref="GeneID:30036890"
/translation="MRGRGVPLQYIIGSQPFGDLDIKCARGVLIPRWETEEWTLKLAQ
LLQYQQLIEQDQNSSRPHSQMTVVDLCTGTGCIPLSLASKLRNTVAYGVDISQKAVKL
FKSNIKSVNRSRRLDKRNSKVIPAQGDISIDPYVFCEKTGITDADIVTANPPYIGRRE
FFSQTERSVRLYEPHQALLGGLEFYRWIYDLSVHLQAKGLVCEVGNAGQIDFMRSLGA
KNGWSSSEFHDLNGHPRAVVMWKDPSWSFLEAL"
gene <369392..>370609
/locus_tag="AWJ20_4728"
/db_xref="GeneID:30036891"
mRNA <369392..>370609
/locus_tag="AWJ20_4728"
/product="putative cysteine synthase"
/transcript_id="XM_018881817.1"
/db_xref="GeneID:30036891"
CDS 369392..370609
/locus_tag="AWJ20_4728"
/inference="similar to AA sequence:KEGG_Orthology:K01738"
/note="Putative cysteine synthase; localized to the
mitochondrial outer membrane; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
16407407]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0004124 - cysteine
synthase activity [Evidence IEA]; GO_function: GO:0003674
- molecular_function [Evidence ND]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0008150 - biological_process [Evidence ND];
GO_process: GO:0008652 - cellular amino acid biosynthetic
process [Evidence IEA]; GO_process: GO:0019344 - cysteine
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0006535 - cysteine biosynthetic process from serine
[Evidence IEA]"
/codon_start=1
/product="putative cysteine synthase"
/protein_id="XP_018736258.1"
/db_xref="GeneID:30036891"
/translation="MTHYSRSEDIVLTIYIRDPDRTMLAKRLVLSSGLLGKGSVSLAA
RGYATIGTAADGGPFVPPFIPLVSESGFPGAVGNTPLVRLNKLSDETGTNIYGKAEFM
NPGGSVKDRAALFIIQDAEKKGLIKPGGTIVEGTAGNTGIGLAHMCRARGYKCVIYMP
NTQSQGKIDHLRRLGAEVYPVPAVAFDNPDNYNHQAKRHADRLDNAVWTNQFDNVANR
QAHIETTGPEIWAQTNGKLDAFTCATGTGGSLAGTTRYLKEISNGRVKAFLADPPGSV
LHSYISSGGKLIERSGSSITEGIGQGRVTDNLKPDIELMDGSLTISDEKTINMVYRLL
DEEGLYLGASSCLNVVAAYEVAQKLGPGSTVVTLLCDSADRYADRLFSRKWLQSKNLD
VLPEHLQKYIVLE"
gene complement(<370763..>371788)
/gene="PPH21"
/locus_tag="AWJ20_4729"
/db_xref="GeneID:30036892"
mRNA complement(<370763..>371788)
/gene="PPH21"
/locus_tag="AWJ20_4729"
/product="phosphoprotein phosphatase 2A catalytic subunit
PPH21"
/transcript_id="XM_018881818.1"
/db_xref="GeneID:30036892"
CDS complement(370763..371788)
/gene="PPH21"
/locus_tag="AWJ20_4729"
/inference="similar to AA sequence:KEGG_Orthology:K04382"
/note="Catalytic subunit of protein phosphatase 2A (PP2A);
functionally redundant with Pph22p; methylated at C
terminus; forms alternate complexes with several
regulatory subunits; involved in signal transduction and
regulation of mitosis; forms nuclear foci upon DNA
replication stress; PPH21 has a paralog, PPH22, that arose
from the whole genome duplication; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 22842922];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22842922]; GO_component: GO:0000159 - protein phosphatase
type 2A complex [Evidence IDA] [PMID 17550305];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA,IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0004721 - phosphoprotein
phosphatase activity [Evidence IEA,IEA]; GO_function:
GO:0004722 - protein serine/threonine phosphatase activity
[Evidence IMP] [PMID 2176150]; GO_process: GO:0000082 -
G1/S transition of mitotic cell cycle [Evidence IGI] [PMID
12518319]; GO_process: GO:0007015 - actin filament
organization [Evidence TAS] [PMID 9001215]; GO_process:
GO:0007117 - budding cell bud growth [Evidence TAS] [PMID
9001215]; GO_process: GO:0007094 - mitotic spindle
assembly checkpoint [Evidence IPI] [PMID 9001215];
GO_process: GO:0006470 - protein dephosphorylation
[Evidence TAS] [PMID 9001215]; GO_process: GO:0006417 -
regulation of translation [Evidence IPI] [PMID 10329624]"
/codon_start=1
/product="phosphoprotein phosphatase 2A catalytic subunit
PPH21"
/protein_id="XP_018736259.1"
/db_xref="GeneID:30036892"
/translation="MEVDQEDPLSQVKHPASTSRSGDGIPEIKPAENLHSIGIGILET
WIERLLKCEILSESQVMRLCDTAREILVNESNVQPVSSPVTICGDIHGQFHDLLELFR
IGGPCPDTNYLFMGDYVDRGYFSVETISLLLAYKVRYPHRITILRGNHESRQITQVYG
FYDECLRKYGNTKVWTAFTDLFDYLPLTALVENRIFCLHGGLSPSIETLDQIVQLDRF
QEAPHEGPVCDLLWSDPDERSAWGVSPRGAGFTFGFDISSQFNHANSLSLIARAHQLI
MEGYQFTHEERVVTIFSAPNYCYRCGNQAAIMEVDENMNTTFLQFDPSPRSGQSNFSR
RTPDYFL"
gene <374864..>376864
/gene="TAF12"
/locus_tag="AWJ20_4730"
/db_xref="GeneID:30036894"
mRNA <374864..>376864
/gene="TAF12"
/locus_tag="AWJ20_4730"
/product="Taf12p"
/transcript_id="XM_018881820.1"
/db_xref="GeneID:30036894"
CDS 374864..376864
/gene="TAF12"
/locus_tag="AWJ20_4730"
/inference="similar to AA sequence:KEGG_Orthology:K03126"
/note="Subunit (61/68 kDa) of TFIID and SAGA complexes;
involved in RNA polymerase II transcription initiation and
in chromatin modification, similar to histone H2A;
GO_component: GO:0000124 - SAGA complex [Evidence IDA]
[PMID 9674426]; GO_component: GO:0046695 - SLIK
(SAGA-like) complex [Evidence IDA] [PMID 12446794];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005669 - transcription factor TFIID
complex [Evidence IEA]; GO_component: GO:0005669 -
transcription factor TFIID complex [Evidence IDA] [PMID
10788514]; GO_component: GO:0005669 - transcription factor
TFIID complex [Evidence IDA] [PMID 15448131]; GO_function:
GO:0001102 - RNA polymerase II activating transcription
factor binding [Evidence IDA,IPI] [PMID 12501245];
GO_function: GO:0001075 - RNA polymerase II core promoter
sequence-specific DNA binding transcription factor
activity involved in preinitiation complex assembly
[Evidence IC]; GO_function: GO:0017025 - TBP-class protein
binding [Evidence IDA] [PMID 10751405]; GO_function:
GO:0003682 - chromatin binding [Evidence IDA] [PMID
10817999]; GO_function: GO:0003682 - chromatin binding
[Evidence IDA] [PMID 12600943]; GO_function: GO:0032947 -
protein complex scaffold [Evidence IMP] [PMID 9674426];
GO_function: GO:0046982 - protein heterodimerization
activity [Evidence IEA]; GO_process: GO:0006352 -
DNA-templated transcription, initiation [Evidence IEA];
GO_process: GO:0051123 - RNA polymerase II transcriptional
preinitiation complex assembly [Evidence IMP] [PMID
12600943]; GO_process: GO:0051123 - RNA polymerase II
transcriptional preinitiation complex assembly [Evidence
IMP] [PMID 12840001]; GO_process: GO:0016568 - chromatin
modification [Evidence IDA,IMP] [PMID 9674426];
GO_process: GO:0016573 - histone acetylation [Evidence
IDA] [PMID 9674426]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0006366 - transcription from RNA polymerase
II promoter [Evidence IMP] [PMID 10751405]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IMP] [PMID 11561287]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IDA] [PMID 12138208]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 12582246]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IDA] [PMID 15448131]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Taf12p"
/protein_id="XP_018736260.1"
/db_xref="GeneID:30036894"
/translation="MNNGNNRAGASGGGGMPNIPNQSPANQAQAVRERLVNLQPQQLN
ALIQQYQQAMQGVIQAGPQSQEGQEHFLRAQRIKQVLQQYQGQMQAQAQQRGQRPPVA
NQAQTAAHAQAQAMNARAQQQAMNAAAANGAIPQNQQAMAQLQQQQLLLQQQQQQQQQ
QQQQQQQQQQQQQQQQQQQQQQQQQQQAAVAAAAAANPNANHARLASQQQQQQQQQQQ
QQQQQQQQQQQQQQRQRQFQSMTPQQQFAYRTSQLEQFRNTQRTLQEQLDRLERALRE
QPNLDEEMKAKIRQQEQSLRDRKDSYKSLELQVMQQIRVAAGQQQLQQQQQQQQQQQQ
QQNPGAQAQVQAQQSQAQTPAAQAQFQQQQQQQAAAAAVRQSPALQNQLPQGSPIGVP
ISSPMNAASTPIQRPGSVASNSSAGRPISPAVGAGAGGPGTPGAPGTPSVNMARMANG
LAPSTRVAMQLSANNIPGGTPPTGPSARPNPPNHLAASQAMSSPTRASVPSLPIPQNL
TVKQPVPVTMKPGRPSLSGGNAIPAAALTTPALTKPPQFEVTGDRVLSKRKLSELVSS
VVGDDKEAMIDGDVEELLLDLADEFVTSVTSFACRLAKHRKSDTLEVKDLQLHLERNW
NIRIPGYSADEIRSVRKIAPTQSYIQKIAGVNMSRSVGGKGI"
gene complement(<377202..>377861)
/locus_tag="AWJ20_4731"
/db_xref="GeneID:30036895"
mRNA complement(<377202..>377861)
/locus_tag="AWJ20_4731"
/product="hypothetical protein"
/transcript_id="XM_018881821.1"
/db_xref="GeneID:30036895"
CDS complement(377202..377861)
/locus_tag="AWJ20_4731"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736261.1"
/db_xref="GeneID:30036895"
/translation="MDLRTFKEASVRGGRASFDWENVKNDKYKTNYLGHSLMAPHQGR
GGSKDPIWYSKEKDGGGGGGEQKNEGEQSELEMMKDMERKALAELLGYDPADMEKLEK
KKDDRSNCEGSSHRSQRSHHHRSSDRDSSRERHRNHHSHRRRDRSNDRDSNKSTRSHE
PRDRHRSRSRSRSRSRTREPSHRESRPRSRDSRKRHSHRHRDRSRERNRDQRDRSRDR
R"
gene <379854..>380492
/gene="SNF7"
/locus_tag="AWJ20_4732"
/db_xref="GeneID:30036896"
mRNA <379854..>380492
/gene="SNF7"
/locus_tag="AWJ20_4732"
/product="ESCRT-III subunit protein SNF7"
/transcript_id="XM_018881822.1"
/db_xref="GeneID:30036896"
CDS 379854..380492
/gene="SNF7"
/locus_tag="AWJ20_4732"
/inference="similar to AA sequence:KEGG_Orthology:K12194"
/note="One of four subunits of the ESCRT-III complex;
involved in the sorting of transmembrane proteins into the
multivesicular body (MVB) pathway; recruited from the
cytoplasm to endosomal membranes; ESCRT-III stands for
endosomal sorting complex required for transport III;
GO_component: GO:0000815 - ESCRT III complex [Evidence
IDA] [PMID 12194857]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 9606181]; GO_component: GO:0005768 -
endosome [Evidence IEA]; GO_component: GO:0010008 -
endosome membrane [Evidence IEA]; GO_component: GO:0016020
- membrane [Evidence IEA]; GO_function: GO:0005515 -
protein binding [Evidence IPI] [PMID 15086794];
GO_process: GO:0071454 - cellular response to anoxia
[Evidence IMP] [PMID 20402793]; GO_process: GO:0070676 -
intralumenal vesicle formation [Evidence IMP] [PMID
19234443]; GO_process: GO:0045324 - late endosome to
vacuole transport [Evidence IMP] [PMID 9606181];
GO_process: GO:0015031 - protein transport [Evidence
IEA,IEA]; GO_process: GO:0006810 - transport [Evidence
IEA]; GO_process: GO:0043162 - ubiquitin-dependent protein
catabolic process via the multivesicular body sorting
pathway [Evidence IC] [PMID 15086794]"
/codon_start=1
/product="ESCRT-III subunit protein SNF7"
/protein_id="XP_018736262.1"
/db_xref="GeneID:30036896"
/translation="MWGYLFGSGAKKDQAKNSIISLREQIDTLSKREKHFQKQIEELE
ATARKNVTTNKAAARTALKRKKEREADLDKTQAQIDSLQQQLHAIENAKLNYETMKIM
SQGAQAMKHIHGNMNIDKVDATMDDIRDQVALGEEISDAISRPLGQEVDEDDLNEELE
ALEQEALDAKMVNAGKAPVHNLPTQQLHEPEPAVEEDDEEEELRKLQAEMAL"
gene complement(<380498..>382045)
/gene="SED5"
/locus_tag="AWJ20_4733"
/db_xref="GeneID:30036897"
mRNA complement(<380498..>382045)
/gene="SED5"
/locus_tag="AWJ20_4733"
/product="Sed5p"
/transcript_id="XM_018881823.1"
/db_xref="GeneID:30036897"
CDS complement(380498..382045)
/gene="SED5"
/locus_tag="AWJ20_4733"
/inference="similar to AA sequence:KEGG_Orthology:K08490"
/note="cis-Golgi t-SNARE syntaxin; required for vesicular
transport between the ER and the Golgi complex; binds at
least 9 SNARE proteins; GO_component: GO:0005794 - Golgi
apparatus [Evidence IEA]; GO_component: GO:0000139 - Golgi
membrane [Evidence IEA]; GO_component: GO:0031201 - SNARE
complex [Evidence IDA] [PMID 11001058]; GO_component:
GO:0031201 - SNARE complex [Evidence IDA] [PMID 11959998];
GO_component: GO:0005801 - cis-Golgi network [Evidence
IDA] [PMID 9725919]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence IDA]
[PMID 1400588]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA,IEA]; GO_function: GO:0005484 - SNAP
receptor activity [Evidence IEA]; GO_function: GO:0005484
- SNAP receptor activity [Evidence IDA] [PMID 11001058];
GO_function: GO:0005484 - SNAP receptor activity [Evidence
IDA] [PMID 11959998]; GO_process: GO:0006888 - ER to Golgi
vesicle-mediated transport [Evidence IMP] [PMID 9545229];
GO_process: GO:0006891 - intra-Golgi vesicle-mediated
transport [Evidence IMP] [PMID 7596416]; GO_process:
GO:0006886 - intracellular protein transport [Evidence
IEA]; GO_process: GO:0015031 - protein transport [Evidence
IEA]; GO_process: GO:0006810 - transport [Evidence IEA];
GO_process: GO:0006906 - vesicle fusion [Evidence IDA]
[PMID 11001046]; GO_process: GO:0048280 - vesicle fusion
with Golgi apparatus [Evidence IMP] [PMID 9545229];
GO_process: GO:0016192 - vesicle-mediated transport
[Evidence IEA]"
/codon_start=1
/product="Sed5p"
/protein_id="XP_018736263.1"
/db_xref="GeneID:30036897"
/translation="MASAVLLPSVQDRTQEFRACVTSIAKINGTVVGRPGSSSSGQTG
SYGGSGIINGPESAFGGPGASSGSGTGLENKSPKSQFALRASGIAQEISETTAMLQRL
ALLAKRKTLFDDRPVEIGELSFVIKQKLSKINDSIASLQAFVKTQGGGKAASAWGTNK
SETQLNEHSRNVVVLLQGKLTDVTTGFTEVLETRTKNTQATRSRTEQFISSASSSVRD
HTSSESPLYAAAANRRGNSSPAGSAATGGAGGDESYGENPYGRPLSSSTAQSLLPDHD
KPGSGQDDFLVLPQQEQTMALLEEQQDSYLSQRSSAVEAIESTIQELGGIFSQLATMV
AEQRETVQRIDANTEDIALNVSGAQRELLKYYSRISSNRWLMVKMFGIIIVSYTGATA
SGGWGSAPDPVAPLAALESARLLTAVTSSSSSSGSSSRRLPHSLTNSIPLTLVGPLVP
PAAGAPPQTLVAPASQEFVPGPSTQRLERSERSNQGLGRSPSRRRQSVRQNYFMYSYI
ECSGPGG"
gene <383190..>384938
/locus_tag="AWJ20_4734"
/db_xref="GeneID:30036898"
mRNA <383190..>384938
/locus_tag="AWJ20_4734"
/product="hypothetical protein"
/transcript_id="XM_018881824.1"
/db_xref="GeneID:30036898"
CDS 383190..384938
/locus_tag="AWJ20_4734"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736264.1"
/db_xref="GeneID:30036898"
/translation="MSQQISQSVPFRYINAIILTPIRSIVNNLNPALQRLACYGLTVY
IGLYYWAPELLESVVRSTVASDLFIRILLPLGLFAAEITCPTTAGVKSLRGGVGDNPG
AYSGVASGAVSSGGAGDAGGAEHDDESKSSLNSAIYREYSANDNDFTGKKDENKKGHN
DEDSVHAASSVVASRGINEGKESATSVGSIDYSGRNNQTSSSWVGKTIPEVDEEESFN
INERDISERDIAEMSVNTSTSGFDVPGLPNPPAASGTTSAVSEPAKDKSPDADVPSDA
SPAGTVGAAAAAQSNDSVHSSNTTPESQTLPVEQSISLSSSQATKFSPSSDCPNSKDQ
KTTDDPTSRHHPLPSLISTSLISDDTKPQSPTQPPPSPPKFIFGNNPGDFTRTNVTED
DLSIMNNLSEGIEKSIESLYKVITPVDANHNDFTVQDYNFSQTWKIGAPKKLIWRDVK
FKEYSPKVLKRIRQLNHIDDLAYSSSFEHILLTKTPAGYTYYSRDKQYIIKPFTNDEF
RTLRASLPLYYQHLLAHPDSSAPRYCGLYKMGNTNFVASTNTRTSPPPASTLAQDARL
PPVIWAHQGLQNQAEY"
gene <386967..>387689
/locus_tag="AWJ20_4735"
/db_xref="GeneID:30036899"
mRNA <386967..>387689
/locus_tag="AWJ20_4735"
/product="hypothetical protein"
/transcript_id="XM_018881825.1"
/db_xref="GeneID:30036899"
CDS 386967..387689
/locus_tag="AWJ20_4735"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736265.1"
/db_xref="GeneID:30036899"
/translation="MPPAAGAPPQTLVAPASQEIFRVGTVSQRLERSERSNPGLGRSP
SRRRQTPVPGESPLTRVTVNRTRAILGEIAGDGSFEAHAKGVSWPEFVDLVVELLDAN
VSGLYDRPEKSYIDPLTGQESSESEMTDQVKSLLRTEFAAGPPFTVQRLAELLGSPLD
FYPAPFVYKYLTALKRVLSVASLSTDYPAVDLQAEIAQSKTDTSSANYGEPGVVMTEI
PWVLDTGLPENTPTAAPADDPF"
gene complement(<387936..>388418)
/gene="RSM24"
/locus_tag="AWJ20_4736"
/db_xref="GeneID:30036900"
mRNA complement(<387936..>388418)
/gene="RSM24"
/locus_tag="AWJ20_4736"
/product="mitochondrial 37S ribosomal protein RSM24"
/transcript_id="XM_018881826.1"
/db_xref="GeneID:30036900"
CDS complement(387936..388418)
/gene="RSM24"
/locus_tag="AWJ20_4736"
/inference="similar to AA sequence:KEGG_Orthology:K17413"
/note="Mitochondrial ribosomal protein of the small
subunit; GO_component: GO:0005763 - mitochondrial small
ribosomal subunit [Evidence IPI] [PMID 11278769];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IPI] [PMID 11278769]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IC] [PMID 11278769]"
/codon_start=1
/product="mitochondrial 37S ribosomal protein RSM24"
/protein_id="XP_018736266.1"
/db_xref="GeneID:30036900"
/translation="MKLTRVELASPYRPPSDESVLTFKYNTFLGEDHPAGKKVTVQFS
PSELGLTAAQKHKLCLLAGARYNSDTDVVTISSSKFPQQAQNKRFLGDILKSLLEAAR
DESDTFADVPLETRHMVAKRRRNKPVRPRVEFPEAWNRPQDAPKPKDDIVSVIHRLPL
"
gene <389936..>390820
/gene="RSM7"
/locus_tag="AWJ20_4737"
/db_xref="GeneID:30036901"
mRNA <389936..>390820
/gene="RSM7"
/locus_tag="AWJ20_4737"
/product="mitochondrial 37S ribosomal protein RSM7"
/transcript_id="XM_018881827.1"
/db_xref="GeneID:30036901"
CDS 389936..390820
/gene="RSM7"
/locus_tag="AWJ20_4737"
/inference="similar to AA sequence:KEGG_Orthology:K02992"
/note="Mitochondrial ribosomal protein of the small
subunit; has similarity to E. coli S7 ribosomal protein;
GO_component: GO:0005763 - mitochondrial small ribosomal
subunit [Evidence IPI] [PMID 11278769]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IEA]; GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IPI] [PMID 11278769]; GO_process:
GO:0032543 - mitochondrial translation [Evidence IC] [PMID
11278769]; GO_process: GO:0006412 - translation [Evidence
IEA]"
/codon_start=1
/product="mitochondrial 37S ribosomal protein RSM7"
/protein_id="XP_018736267.1"
/db_xref="GeneID:30036901"
/translation="MSWVAKSSSVLAKSFGQRLFQSGSPSLLALRYNSTAASPKPSSA
STTPSTASNQPSTAKYLVSLTEKELGEEGITRESADKEAQQWIDALNEVRNEFYAEGG
FSPNKTFAPPGSTDFQFFRDVPRETPRSFELTAEQMEQYERIKEKAIPVRNDPTIEYL
TKLIMRHGLKARAQKYMSQALYLVHLRTRQDPVALLKDTLEKMAPLVKLKRYTDGGAR
AEMIPVPLSERHRLRQSWLWLLESSRKRPSKDFSVRLSEEILSAIQGKSPGFEKRTQQ
HKLAIVNRSYVSLMTRKR"
gene <393587..>394708
/gene="HMO1"
/locus_tag="AWJ20_4738"
/db_xref="GeneID:30036902"
mRNA join(<393587..393694,393767..>394708)
/gene="HMO1"
/locus_tag="AWJ20_4738"
/product="Hmo1p"
/transcript_id="XM_018881828.1"
/db_xref="GeneID:30036902"
CDS join(393587..393694,393767..394708)
/gene="HMO1"
/locus_tag="AWJ20_4738"
/note="Chromatin associated high mobility group (HMG)
family member; involved in genome maintenance;
rDNA-binding component of the Pol I transcription system;
associates with a 5'-3' DNA helicase and Fpr1p, a prolyl
isomerase; relocalizes to the cytosol in response to
hypoxia; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 8969238]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 22932476]; GO_component: GO:0000790 -
nuclear chromatin [Evidence IDA] [PMID 16612005];
GO_component: GO:0000790 - nuclear chromatin [Evidence
IDA] [PMID 17646381]; GO_component: GO:0005730 - nucleolus
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 12374750]; GO_component: GO:0005634 -
nucleus [Evidence IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22932476]; GO_component: GO:0032040 -
small-subunit processome [Evidence IDA] [PMID 22553361];
GO_function: GO:0003677 - DNA binding [Evidence IEA];
GO_function: GO:0008301 - DNA binding, bending [Evidence
IDA] [PMID 15507436]; GO_function: GO:0008301 - DNA
binding, bending [Evidence IDA] [PMID 16533046];
GO_function: GO:0003690 - double-stranded DNA binding
[Evidence IDA] [PMID 15507436]; GO_function: GO:0003690 -
double-stranded DNA binding [Evidence IDA] [PMID
16533046]; GO_function: GO:0000400 - four-way junction DNA
binding [Evidence IDA] [PMID 15507436]; GO_function:
GO:0000400 - four-way junction DNA binding [Evidence IDA]
[PMID 16533046]; GO_process: GO:0060962 - regulation of
ribosomal protein gene transcription from RNA polymerase
II promoter [Evidence IMP] [PMID 17875934]; GO_process:
GO:0060962 - regulation of ribosomal protein gene
transcription from RNA polymerase II promoter [Evidence
IGI,IMP] [PMID 18187511]; GO_process: GO:0034401 -
regulation of transcription by chromatin organization
[Evidence IDA] [PMID 18451108]; GO_process: GO:0006356 -
regulation of transcription from RNA polymerase I promoter
[Evidence IGI] [PMID 12374750]; GO_process: GO:0006356 -
regulation of transcription from RNA polymerase I promoter
[Evidence IMP] [PMID 16612005]; GO_process: GO:0006355 -
regulation of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0006351 - transcription, DNA-templated
[Evidence IEA]"
/codon_start=1
/product="Hmo1p"
/protein_id="XP_018736268.1"
/db_xref="GeneID:30036902"
/translation="MSVSDPLIQCYRWIARPFAEILNRILAISCEAGATRLISALFTL
SKAAQEASGATIDFYNQLSGTSSGFPSSLSDLSSILASSATDLSLAHTDADFTAATAG
LDVGSGSVVDPTGLSTPANDADEDAEGGKKKKAKRQRAPVDPNAPKKPVTAFFLYVAA
NRKHVQADLQAAKPEGEIVGNHELTAELGVRWHALSEAERASWKNQYKELMDIYNVEK
QAYDSKKALTGDVNGVNGIGNLVVPGKATPVKKQANPVKKQRKDGLPGPNVVPGPGIP
LGNELPGSLVHPQHHQVQHQVQHPGQHPGQHPGPLHTPGQLQSPLSGDKAKRDKKKEK
KRRKPELPTDAPPTI"
gene <400511..>402397
/gene="ARE2"
/locus_tag="AWJ20_4739"
/db_xref="GeneID:30036903"
mRNA <400511..>402397
/gene="ARE2"
/locus_tag="AWJ20_4739"
/product="Are2p"
/transcript_id="XM_018881829.1"
/db_xref="GeneID:30036903"
CDS 400511..402397
/gene="ARE2"
/locus_tag="AWJ20_4739"
/inference="similar to AA sequence:KEGG_Orthology:K11155"
/note="Acyl-CoA:sterol acyltransferase; endoplasmic
reticulum enzyme that contributes the major sterol
esterification activity in the presence of oxygen; ARE2
has a paralog, ARE1, that arose from the whole genome
duplication; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IDA] [PMID 10672016];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_function: GO:0008374 -
O-acyltransferase activity [Evidence IEA]; GO_function:
GO:0034736 - cholesterol O-acyltransferase activity
[Evidence IEA]; GO_function: GO:0034737 - ergosterol
O-acyltransferase activity [Evidence IGI,IMP] [PMID
10672016]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_function: GO:0016746 - transferase
activity, transferring acyl groups [Evidence IEA];
GO_process: GO:0008204 - ergosterol metabolic process
[Evidence IGI,IMP] [PMID 10672016]"
/codon_start=1
/product="Are2p"
/protein_id="XP_018736269.1"
/db_xref="GeneID:30036903"
/translation="MEQGSTSTMSSLVYLPRQKPFHRKLTPTTSPLAMSPNLHGISTG
TSYGKQLGGKLADKDKLSSSHYNSNPNSSPNSNSNSYSYTDSEVLSPAQIHSQTQTQI
LEPPRFHTHSPHSRAHTHASGLSHKSNTGLTRKKSNSSTSGYLPHYKPIHTKSSASIL
SLSKHSPTSSYTGFRHLAMIVLTLGNLRLMIHDYQTYGFIMTLYRLGISQSDLNVAGL
LLLSTPIHLFIALLIELGASKTVSNANHTTSEADNAHQVDSTSGITTGSRLGSKENSS
VDVPTSTTKNSKASSNPTEPNKPHKKYLWKLFAALHTINATLVLIISSYTVYTSIWHP
LIGTVIECHSIILCLKVSSYALTNRDLRDAYLEDAPVPELYGPSKYPHNLTIGNLVYF
WWAPTLVYQPEYPRAPKVRYGFLVNRVLEMVGTIVLIWFLSGQYAVPILESSLVHFQN
ADLGQICETIMRLSTVSIVVWLLGFFFIFQSYLNFLAELLRFGDRDFYQDWWNAGSVG
TYWRLWNKPVSNYFRRHFYAPLLKRGWTPMSSSLVVFFISAVLHELVVGIPTRNVIGV
AFVCMMLQVPLIMVTAPLEKMRGPATTVGNCIFWLSFFLGQPVAVLMYYFAWNVKNGT
HIKI"
gene complement(<403065..>404108)
/locus_tag="AWJ20_4740"
/db_xref="GeneID:30036905"
mRNA complement(<403065..>404108)
/locus_tag="AWJ20_4740"
/product="hypothetical protein"
/transcript_id="XM_018881831.1"
/db_xref="GeneID:30036905"
CDS complement(403065..404108)
/locus_tag="AWJ20_4740"
/inference="similar to AA sequence:KEGG_Orthology:K10082"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736270.1"
/db_xref="GeneID:30036905"
/translation="MNPVTLLVAVLSLVAGSVEARRRPQTVDVSRFPNTENPFSRTES
LAHSMEKPYLDKNFQSRYYDYGGSTFIRGDKYIRLTPDLPDQRGWLMSKIPSMPDNFQ
IEVEFGIDGQGTSIYGDGMAIWLLNERVTQEGPVFGLRDNFKGTAIFLDTYKNNGPGK
VFPYAMIMHGDGQTSYDQAHDGLANEIAGCSVRGLHNGREGNRIRITYVQGKFLSLET
DYRGKHKWEKCFETNQVTINKPAFLGFSAQTGELSEYHDIYSIKVYSLDNPPASYAEV
DYYLNGNKNAPIHESYSSHSSSSSSSSSSSSSGWGSFFLKTFLFILVVAGGYVGFTLY
RAKQRRERDAFYL"
gene complement(<404681..>406552)
/gene="SKN7"
/locus_tag="AWJ20_4741"
/db_xref="GeneID:30036906"
mRNA complement(<404681..>406552)
/gene="SKN7"
/locus_tag="AWJ20_4741"
/product="kinase-regulated stress-responsive transcription
factor SKN7"
/transcript_id="XM_018881832.1"
/db_xref="GeneID:30036906"
CDS complement(404681..406552)
/gene="SKN7"
/locus_tag="AWJ20_4741"
/inference="similar to AA sequence:KEGG_Orthology:K15859"
/codon_start=1
/product="kinase-regulated stress-responsive transcription
factor SKN7"
/protein_id="XP_018736271.1"
/db_xref="GeneID:30036906"
/translation="MSSGRWDSPNASGSWLDMHMSMGQPPPPGQDQDHDHTDPMDLSQ
GQDAGHGTSHGQPVRNISPTQQHARNIQVPIQDTHPNNMNMKSELDYTRHQVNPNTGT
GPAGPNEPSRESKDFVKKLFTMLEDPTHRHIVRWSNSEDSFVVLDASEFTKEVLPRHF
KHSNFASFVRQLNKYDFHKIRANVSKSSGGDNYPDNSWEFHHPDFNPKNKDQLDKIKR
KVPGKKVHPAGGVGGSGNSNTPSLTDVNGHGLVSIGQEKVDSISYELENARKLNGFLA
ARVEQLEDDSRTMSKNLSLIHDAFLMQTSYLQRMLSLLAMNDPNGQFSELLDSLSKDG
GYSGISSSVSHELQLQQQKQQHQHLQNQHLQQQQQQTQQQQPPQLTHQLPQIRVPPPQ
QQPQPPHQLPPQQQQQQQPSQQQQQPTQQSNMPSALPSSATSTSLSPSIPNSNRINQV
GTTSESNSQFHVLLADEDDNSVLICRRMLLQYGCEVDVANDGLAAVQRAESTQYDLIL
MDMILPQLDGMSATELIRHSDQTTPIIAMMSTTDSDGQLDSRTVEKYRSKGVTMILPK
PFSKSVLYSTLSIFLKPVSTTTHHPGQPDRLGYMAPSARLRDPTTDSLGSQTKKHRY"
gene complement(<412627..>414981)
/gene="SCH9"
/locus_tag="AWJ20_4742"
/db_xref="GeneID:30036907"
mRNA complement(<412627..>414981)
/gene="SCH9"
/locus_tag="AWJ20_4742"
/product="serine/threonine protein kinase SCH9"
/transcript_id="XM_018881833.1"
/db_xref="GeneID:30036907"
CDS complement(412627..414981)
/gene="SCH9"
/locus_tag="AWJ20_4742"
/inference="similar to AA sequence:KEGG_Orthology:K08286"
/codon_start=1
/product="serine/threonine protein kinase SCH9"
/protein_id="XP_018736272.1"
/db_xref="GeneID:30036907"
/translation="MPDFAKSLFSFGGFSKSSNSSQNSSPTGSANVSPGLHPLKDKPI
SAVTQSNAAPTFRSSTSSAATALSLGFGRSGGSGSGGSEDQMVLSDEDNGSASGGAGG
AGAANSGLATARPSTVDVGGSDSANSNAVVTILGNDTQANGLGAVTTVPEAGLSSVRP
MGKLRVRIIEARGLVAQPEGSIPYVVCTYGTSEFISHGPTGTGSGGDPSVSAGAGANS
TQPIGIAIPKSRQSSSLTLSKLNNGAHRGAGSNTNPMWKDEAQFDVTSNDGELEVSIY
DAKNNDLFLGHARLKPDLTSRVIEKWLTLEPRIQGERVSGEVRVGIFYEKVSKRQYGP
QDFEVLRLVGKGTFGQVYQVRKKDTQRIYAMKVLSKKAIVQKKEIAHTIGERNILVRT
SSANSAFIVGLKMSFQTPTDLYLVTDYMSGGELFWHLQKEGRFSEQRAKFYIAELIVA
LEHLHDNDIVYRDLKPENILLDANGHIALCDFGLSKANLNDNGTTNTFCGTTEYLAPE
VLLDDQGYTKMVDFWSLGVLVFEMCCGWSPFYAEDNQQMYKNIAFGKVRFPKDALSPD
GRNFVKGLLNRNPKHRLGATDDARELRAHPFFSDINWDLLIAKKIPPPFKPQVSSETD
TSNFDPEFTQASTSVLHRAFMPVSSTPLSPGIQANFQGFTYVDDSSLDENFRSRLGTS
VRPKSHIQHDDDIDLYDDKDIKISESYYNESDLMARSIKRTGPLSGLGPHGSSGLKPP
GHSAQGHSQGHNQGHNNDGDYDADVDIMMEDDEHDFVNGQGFEM"
gene complement(<417173..>418498)
/gene="ALG1"
/locus_tag="AWJ20_4743"
/db_xref="GeneID:30036908"
mRNA complement(<417173..>418498)
/gene="ALG1"
/locus_tag="AWJ20_4743"
/product="chitobiosyldiphosphodolichol beta-1,4
mannosyltransferase"
/transcript_id="XM_018881834.1"
/db_xref="GeneID:30036908"
CDS complement(417173..418498)
/gene="ALG1"
/locus_tag="AWJ20_4743"
/inference="similar to AA sequence:KEGG_Orthology:K03842"
/note="Mannosyltransferase; involved in asparagine-linked
glycosylation in the endoplasmic reticulum (ER); essential
for viability, mutation is functionally complemented by
human ortholog; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IPI] [PMID 15044395];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISS]
[PMID 2182636]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_function: GO:0019187 -
beta-1,4-mannosyltransferase activity [Evidence IDA] [PMID
6368538]; GO_function: GO:0004578 -
chitobiosyldiphosphodolichol beta-mannosyltransferase
activity [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_function:
GO:0016757 - transferase activity, transferring glycosyl
groups [Evidence IEA,IEA]; GO_process: GO:0009058 -
biosynthetic process [Evidence IEA]; GO_process:
GO:0097502 - mannosylation [Evidence IEA]; GO_process:
GO:0006490 - oligosaccharide-lipid intermediate
biosynthetic process [Evidence IMP] [PMID 7037780];
GO_process: GO:0006487 - protein N-linked glycosylation
[Evidence IMP] [PMID 6369318]; GO_process: GO:0006486 -
protein glycosylation [Evidence IEA]"
/codon_start=1
/product="chitobiosyldiphosphodolichol beta-1,4
mannosyltransferase"
/protein_id="XP_018736273.1"
/db_xref="GeneID:30036908"
/translation="MNYHALSIAKCGYEVDLCGYKESKLMDEVIENPLITVREIPVVK
NAYKLPFVLFAPYKVVKQHWILYDLLKQLKRADFLLVQNPPSIPVLGMVRFFSLFMSP
RTKVIIDWHNLGYSILALKFPDNPNHPFVKIYKWYENWFGYRSFVHLTVTVALGQFLR
TEFAMNAKRIIPLYDRPASQFSLLSESQRKEIISKYQTTLFKDFDAKTEKILVTSTSY
TPDEDLTILLEALEKYSLASNPSSQTTEEKPTSDDNKSESKKSQILTRSSASTQLPPL
RVIITGKGPMFKETRAKIDALQPTLHNISIHTTWLPIEDYPKVLGAADLGISLHQSSS
GWDLPMKIVDMFGCGVPVISLSFPAISELVKHSENGLIVDTSTDMAKQLAKLFTNPAT
LNNLKRGAVRESRIKWDTNWTEKLGPLFGVGQYREPAPGEDDSSSSDDD"
gene complement(<419425..>420120)
/locus_tag="AWJ20_4744"
/db_xref="GeneID:30036909"
mRNA complement(<419425..>420120)
/locus_tag="AWJ20_4744"
/product="calmodulin"
/transcript_id="XM_018881835.1"
/db_xref="GeneID:30036909"
CDS complement(419425..420120)
/locus_tag="AWJ20_4744"
/codon_start=1
/product="calmodulin"
/protein_id="XP_018736274.1"
/db_xref="GeneID:30036909"
/translation="MWPSPLASASLIISSTSSSVKRSPIVVMTWRNSAAEINPLPSRS
NTLNASRISSSESVSFIFRAIMVRNSGKSIVPSLSTSTSWIISDNSLSEGFNAKLLMT
VPSSLVVILPGCVRFSRGWGVAFGGWGSAPDPGCSCFAGDGGDSGGNDSSEARGAAGS
GAQPQPPEARGERTIVVLIEQRKCLFKRGDLLFGQLVSLFGLLVPVSSLITQPLSPMS
RSVISPVTGLKTL"
gene <419597..>420163
/gene="CMD1"
/locus_tag="AWJ20_4745"
/db_xref="GeneID:30036910"
mRNA <419597..>420163
/gene="CMD1"
/locus_tag="AWJ20_4745"
/product="calmodulin"
/transcript_id="XM_018881836.1"
/db_xref="GeneID:30036910"
CDS 419597..420163
/gene="CMD1"
/locus_tag="AWJ20_4745"
/inference="similar to AA sequence:KEGG_Orthology:K02183"
/note="Calmodulin; Ca++ binding protein that regulates
Ca++ independent processes (mitosis, bud growth, actin
organization, endocytosis, etc.) and Ca++ dependent
processes (stress-activated pathways), targets include
Nuf1p, Myo2p and calcineurin; GO_component: GO:0005935 -
cellular bud neck [Evidence IDA] [PMID 1639847];
GO_component: GO:0005934 - cellular bud tip [Evidence IDA]
[PMID 1639847]; GO_component: GO:0005823 - central plaque
of spindle pole body [Evidence IDA] [PMID 8601600];
GO_component: GO:0005823 - central plaque of spindle pole
body [Evidence IDA] [PMID 8886974]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005856 - cytoskeleton [Evidence IEA]; GO_component:
GO:0000131 - incipient cellular bud site [Evidence IDA]
[PMID 1639847]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 1639847];
GO_component: GO:0005816 - spindle pole body [Evidence
IEA]; GO_function: GO:0005509 - calcium ion binding
[Evidence IEA]; GO_function: GO:0005509 - calcium ion
binding [Evidence IDA] [PMID 3533275]; GO_function:
GO:0005509 - calcium ion binding [Evidence IDA] [PMID
8461293]; GO_function: GO:0048306 - calcium-dependent
protein binding [Evidence IDA] [PMID 1321337];
GO_function: GO:0048306 - calcium-dependent protein
binding [Evidence IPI] [PMID 9756868]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0005515 - protein binding [Evidence IPI]
[PMID 10749918]; GO_function: GO:0005515 - protein binding
[Evidence IPI] [PMID 7925277]; GO_function: GO:0005515 -
protein binding [Evidence IPI] [PMID 8247006];
GO_function: GO:0005515 - protein binding [Evidence IPI]
[PMID 8294515]; GO_function: GO:0005515 - protein binding
[Evidence IPI] [PMID 9450989]; GO_process: GO:0006607 -
NLS-bearing protein import into nucleus [Evidence IMP]
[PMID 17878171]; GO_process: GO:0007114 - cell budding
[Evidence IMP] [PMID 8310294]; GO_process: GO:0007010 -
cytoskeleton organization [Evidence IMP] [PMID 8310294];
GO_process: GO:0000742 - karyogamy involved in conjugation
with cellular fusion [Evidence IGI,IMP] [PMID 12836012];
GO_process: GO:0016237 - microautophagy [Evidence IMP]
[PMID 16055436]; GO_process: GO:0006661 -
phosphatidylinositol biosynthetic process [Evidence
IGI,IMP] [PMID 12079494]; GO_process: GO:0006898 -
receptor-mediated endocytosis [Evidence IMP] [PMID
7988551]; GO_process: GO:0006898 - receptor-mediated
endocytosis [Evidence IMP] [PMID 9450989]; GO_process:
GO:0051300 - spindle pole body organization [Evidence IMP]
[PMID 1469052]; GO_process: GO:0042991 - transcription
factor import into nucleus [Evidence IMP] [PMID 17878171];
GO_process: GO:0042144 - vacuole fusion, non-autophagic
[Evidence IDA,IMP] [PMID 9859992]"
/codon_start=1
/product="calmodulin"
/protein_id="XP_018736275.1"
/db_xref="GeneID:30036910"
/translation="MVRSPRASGGWGCAPDPAAPLASLESLPPESPPSPAKQEQPGSG
AEPQPPKATPHPRENLTQPGKITTKELGTVMRSLALNPSESELSDMIHEVDVDNDGTI
DFPEFLTMMARKMKDTDSEEEIREAFKVFDRDGNGFISAAELRHVMTTIGERLTEEEV
DEMIREADANGDGHIDYNEFVNLLQNTK"
gene <424057..>427372
/locus_tag="AWJ20_4746"
/db_xref="GeneID:30036911"
mRNA join(<424057..424204,424335..>427372)
/locus_tag="AWJ20_4746"
/product="hypothetical protein"
/transcript_id="XM_018881837.1"
/db_xref="GeneID:30036911"
CDS join(424057..424204,424335..427372)
/locus_tag="AWJ20_4746"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736276.1"
/db_xref="GeneID:30036911"
/translation="MSSSGIGSFLKESRDKLNDVSAKGKQKYDSGDWKKAPKFSLPSS
RIGRQARGATRVWGGAPAAGGNLTPVVEVTNRTSEENERESIQARPLSALTDPSLFPP
PPKHRGYENGAPVQPAQPVAPVQPVQAAHPGYGQQQQPQTGYAPSPAAGYGQLPPQAP
AQVPGQAPGVAPGQTYQAYGQNQGQLPPQQQQQQPQVQGYNPNPAAGYGQVASPGQPG
SYQQQQQLSGYGQPGQLGQPGVIPAPSPPVQQPPQQYGQPQSFQQPPQQQYGQQPAQQ
QYGQQPAQQQYGQQPAQQQYGQQPAQQQPAPQYGQEYGQQQPAQPYAQQYGQQAPQQI
VPQAAPQPTPVEQPPNYPSQNAAPSPAPALSPAPPARPPLPDPTSFPKPPVFGNPASA
AINSPNSTTRAPATPGVGAPEDAQSKPVKAPLPDPASFAPPPVFGRPGSAASSAVASP
GVNRSSPSKPGAGMPPPPPPRQNLPDPTSFAPPPIYGNPASASYKAPAPQAAAATITA
TQQPQHQQQQQLPPPVSRQTPTPPASRVSPTPPATRATPPGSATHSTGPRQAPPKPAS
LKVQPTPPPKPSAFSTSGNTNTVPHPQPARTYPSQQQLPQPLQSQFQGQPQGQPPAYA
LTDSLNNLSVNDNQNEVQAVPSPSGDSQSSAPQPAVAFAQPGIPNFAAEIAARKTRNS
VSHSSTASASPPPISPASTGASINKKAPPPPPKKKPSLSTGSSFSAASTPAPAAPGTG
YAHSSVASPPVIAPKPIVPSAPARNHPHTVGVNSSHAPSLPPPINHATRPSVETIARS
GSFSDSQVQPEPKEPKRFDFQLATAWFAVPMGQPLHFPPDLSGTEYTYSTSSSGSTRT
LKIALRITEDLSVVRFTMTWDESDPVRTCRVTRTDSAVPRLLTGPELVYSHSQYGELV
ASWAESKMGQQVGNGECWTLAQQAIEQSTHGQAMTSKGLVHGMLIYGTNPAISREEIR
RGDIIQFREARFEKRDPTTGRVVSQSLSGAPDHTSVVTSVDSNTIHVVHQNVSNVRRV
QPGEYVLSDFVSGDIKIYRVVAKDWVEALSV"
gene <428316..>429392
/locus_tag="AWJ20_4747"
/db_xref="GeneID:30036912"
mRNA <428316..>429392
/locus_tag="AWJ20_4747"
/product="hypothetical protein"
/transcript_id="XM_018881838.1"
/db_xref="GeneID:30036912"
CDS 428316..429392
/locus_tag="AWJ20_4747"
/inference="similar to AA sequence:KEGG_Orthology:K07119"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736277.1"
/db_xref="GeneID:30036912"
/translation="MSLQIKELVLENPPVTETNLVLGQETSTFKEVTSTLDPESLKTG
DIVVQPLIFSNDPTQRAWIQKGLDPKRMYVPPVLKGEAMRSLGAGKVIVSKSDKYKVG
DIVIGLLSWSNYSLVNELRISTKVSDPNADLPTYLSLVGASGLAAYYGLTEVGRVKPE
HTVVVSAASGATGIIAVQYAKHVIGCKKVIGITGSDDKIDHVKKVGADVAVNYKDPEF
KKQLSDAIGPDFADVYFDNVGGEILDFMLSKVKPFGVVIACGAIAGYNDHSKGFIRNW
GQIITNRLRVEGFIVTDFKDKHAETFVKLFTALQQGKISLSDSINLVDLSSESNSLAK
IPEVWGQLFSGNKVGKLLTRVSKI"
gene complement(<429684..>431108)
/gene="HRT3"
/locus_tag="AWJ20_4748"
/db_xref="GeneID:30036913"
mRNA complement(<429684..>431108)
/gene="HRT3"
/locus_tag="AWJ20_4748"
/product="SCF ubiquitin ligase complex subunit HRT3"
/transcript_id="XM_018881839.1"
/db_xref="GeneID:30036913"
CDS complement(429684..431108)
/gene="HRT3"
/locus_tag="AWJ20_4748"
/inference="similar to AA sequence:KEGG_Orthology:K10295"
/note="Putative SCF-ubiquitin ligase F-box protein; based
on both genetic and physical interactions and sequence
similarity; identified in association with Cdc53p, Skp1p
and Ubi4 in large and small-scale studies; GO_component:
GO:0019005 - SCF ubiquitin ligase complex [Evidence IDA]
[PMID 10582239]; GO_component: GO:0019005 - SCF ubiquitin
ligase complex [Evidence IDA,IPI] [PMID 14747994];
GO_component: GO:0019005 - SCF ubiquitin ligase complex
[Evidence IPI] [PMID 17141224]; GO_function: GO:0030674 -
protein binding, bridging [Evidence ISA] [PMID 10582239];
GO_function: GO:0030674 - protein binding, bridging
[Evidence ISA] [PMID 9635407]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence ISA]
[PMID 10582239]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence ISA]
[PMID 9635407]; GO_process: GO:0031146 - SCF-dependent
proteasomal ubiquitin-dependent protein catabolic process
[Evidence IGI,ISA] [PMID 10582239]; GO_process: GO:0031146
- SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [Evidence ISA] [PMID 9635407];
GO_process: GO:0071406 - cellular response to
methylmercury [Evidence IMP] [PMID 17141224]; GO_process:
GO:0016567 - protein ubiquitination [Evidence IEA]"
/codon_start=1
/product="SCF ubiquitin ligase complex subunit HRT3"
/protein_id="XP_018736278.1"
/db_xref="GeneID:30036913"
/translation="MSELDRFRQAWRSEVQHRHSDGQNSTAPSATSSTATNGRTAASN
NAGEAAINRDTTDDISTERALSPTRVITPDSAFRAERPEHVYYNKDLSRTDLDALEAF
ERAVDSEHTGKLSDAVRHYRDAFRLNERVDKLYREKYYSIKPTHKKPSTKSSGGAESS
TKTSTKKSQKVLLGDGTGSVSELSKNLAELSLTAEDEELPCPILQVPVEILSEIFRYL
ALDDLSSFTKCTYSCKSFAHIGYTTKSIWMALCQQAYSKQHYTEDAVEELCNGNELEA
VKKYWGNSFRRMYLERPRIRFDGVYISTCNYLRPGVGDTWNAPIHMVTYYRYCKFYED
GTCINLLTTSEPSDVVPVFTRNITGTAHGTGPTRPSKKQPAVIYTKEDGTVLSRPKGI
MSGTWKINGLDGTVLIESEGSVERYTFYMQLQIRSSGHNRHNKLKWVKFWSINNLSDT
YGEFSLRHDKAFFFLKRHPAALTT"
gene <434099..>436300
/gene="SPA2"
/locus_tag="AWJ20_4749"
/db_xref="GeneID:30036914"
mRNA <434099..>436300
/gene="SPA2"
/locus_tag="AWJ20_4749"
/product="Spa2p"
/transcript_id="XM_018881840.1"
/db_xref="GeneID:30036914"
CDS 434099..436300
/gene="SPA2"
/locus_tag="AWJ20_4749"
/note="Component of the polarisome; functions in actin
cytoskeletal organization during polarized growth; acts as
a scaffold for Mkk1p and Mpk1p cell wall integrity
signaling components; potential Cdc28p substrate; coding
sequence contains length polymorphisms in different
strains; SPA2 has a paralog, SPH1, that arose from the
whole genome duplication; GO_component: GO:0005938 - cell
cortex [Evidence IDA] [PMID 23673619]; GO_component:
GO:0051286 - cell tip [Evidence IEA]; GO_component:
GO:0005935 - cellular bud neck [Evidence IDA] [PMID
9214378]; GO_component: GO:0005934 - cellular bud tip
[Evidence IDA] [PMID 12361575]; GO_component: GO:0005934 -
cellular bud tip [Evidence IDA] [PMID 2647769];
GO_component: GO:0000131 - incipient cellular bud site
[Evidence IDA] [PMID 9214378]; GO_component: GO:0043332 -
mating projection tip [Evidence IDA] [PMID 2647769];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 9214378]; GO_component: GO:0000133 - polarisome
[Evidence IDA] [PMID 9632790]; GO_function: GO:0005078 -
MAP-kinase scaffold activity [Evidence IDA,IMP] [PMID
12361575]; GO_process: GO:0007121 - bipolar cellular bud
site selection [Evidence IMP] [PMID 8909546]; GO_process:
GO:0007118 - budding cell apical bud growth [Evidence
IGI,IMP] [PMID 10866679]; GO_process: GO:0030010 -
establishment of cell polarity [Evidence IMP] [PMID
8013906]; GO_process: GO:0030010 - establishment of cell
polarity [Evidence IMP] [PMID 8909546]; GO_process:
GO:0036267 - invasive filamentous growth [Evidence IGI]
[PMID 9443897]; GO_process: GO:0031382 - mating projection
assembly [Evidence IGI] [PMID 9443897]; GO_process:
GO:2000251 - positive regulation of actin cytoskeleton
reorganization [Evidence IGI,IMP] [PMID 12857882];
GO_process: GO:2000251 - positive regulation of actin
cytoskeleton reorganization [Evidence IMP] [PMID
19633059]; GO_process: GO:0007124 - pseudohyphal growth
[Evidence IMP] [PMID 9055077]; GO_process: GO:0007124 -
pseudohyphal growth [Evidence IMP] [PMID 9443897];
GO_process: GO:0008360 - regulation of cell shape
[Evidence IEA]; GO_process: GO:0031384 - regulation of
initiation of mating projection growth [Evidence IMP]
[PMID 14734532]; GO_process: GO:0032880 - regulation of
protein localization [Evidence IMP] [PMID 10085294];
GO_process: GO:0032880 - regulation of protein
localization [Evidence IMP] [PMID 11740491]; GO_process:
GO:0032880 - regulation of protein localization [Evidence
IMP] [PMID 12857882]; GO_process: GO:0032880 - regulation
of protein localization [Evidence IMP] [PMID 16166638];
GO_process: GO:0032880 - regulation of protein
localization [Evidence IMP] [PMID 8909546]; GO_process:
GO:0032880 - regulation of protein localization [Evidence
IMP] [PMID 9571251]; GO_process: GO:0032880 - regulation
of protein localization [Evidence IMP] [PMID 9632790];
GO_process: GO:0031385 - regulation of termination of
mating projection growth [Evidence IMP] [PMID 14734532]"
/codon_start=1
/product="Spa2p"
/protein_id="XP_018736279.1"
/db_xref="GeneID:30036914"
/translation="MSSVNYNQPIHSRGPSLDKYSGMTNSHSNSNLYGANGNGNPNTS
PNPTDGSIHTTRSSTDDVSIDDTIASYHHTFCQFTGHYNGIWDGSRGDGNGGPSAPGS
KARQKLEKLSKVHFSELSTDVYDELVRRVDPLPHETEFLAPRDNIHRKRNQARQKLAM
LSPSRFNDLASDILYEIERRYPNLRANPPADRPSISDPNDPNSAKESPALGSTSQSPY
LTTRRFSNASAVSNATSRYTSEDYHLRDSVMPPRNSSLNTLGISSPAATPPMPSESAF
THQHNASIVPPLQEEVSIPPSNRSAPLAQPIKSTTIIPTKSTLVEEDDDDDEEGFEDT
IDADDEADESAGIANNSTFSSNGSVRNSKRLSMLTPLSDIEEEADAARLAAVAAAANG
GVTGDRGVHDVAEDEITRGGSYGNEQDSAGGIEEGEESDDESVSAKLRDRDEQIQMLV
AEGSRMDETINNLESRLQESETVKTTLEEENNRLHDMVGELEEQRQKAVDEKESIQKR
LEALEQEVEESKAAIAAHEATINSHKETIDKLGDEKKQSEDELSRATGLLTQLREEHA
NDSDLQQANARHVETIAGHETTIDNLQRELDSTKETHSREIDDLKQQHESQIAELTTS
HSTLKDQVDSHANEKEELTKRHQQELDELVNSHSMAIQQYKTEAEEHKANLVLLQESH
SALEQEHNNIKDRHAESLSKQNEYAESSATLSNDYLLLQNKLTEHETVSSY"
gene <436538..>437770
/gene="SPA2"
/locus_tag="AWJ20_4750"
/db_xref="GeneID:30036916"
mRNA <436538..>437770
/gene="SPA2"
/locus_tag="AWJ20_4750"
/product="Spa2p"
/transcript_id="XM_018881842.1"
/db_xref="GeneID:30036916"
CDS 436538..437770
/gene="SPA2"
/locus_tag="AWJ20_4750"
/note="Component of the polarisome; functions in actin
cytoskeletal organization during polarized growth; acts as
a scaffold for Mkk1p and Mpk1p cell wall integrity
signaling components; potential Cdc28p substrate; coding
sequence contains length polymorphisms in different
strains; SPA2 has a paralog, SPH1, that arose from the
whole genome duplication; GO_component: GO:0005938 - cell
cortex [Evidence IDA] [PMID 23673619]; GO_component:
GO:0051286 - cell tip [Evidence IEA]; GO_component:
GO:0005935 - cellular bud neck [Evidence IDA] [PMID
9214378]; GO_component: GO:0005934 - cellular bud tip
[Evidence IDA] [PMID 12361575]; GO_component: GO:0005934 -
cellular bud tip [Evidence IDA] [PMID 2647769];
GO_component: GO:0000131 - incipient cellular bud site
[Evidence IDA] [PMID 9214378]; GO_component: GO:0043332 -
mating projection tip [Evidence IDA] [PMID 2647769];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 9214378]; GO_component: GO:0000133 - polarisome
[Evidence IDA] [PMID 9632790]; GO_function: GO:0005078 -
MAP-kinase scaffold activity [Evidence IDA,IMP] [PMID
12361575]; GO_process: GO:0007121 - bipolar cellular bud
site selection [Evidence IMP] [PMID 8909546]; GO_process:
GO:0007118 - budding cell apical bud growth [Evidence
IGI,IMP] [PMID 10866679]; GO_process: GO:0030010 -
establishment of cell polarity [Evidence IMP] [PMID
8013906]; GO_process: GO:0030010 - establishment of cell
polarity [Evidence IMP] [PMID 8909546]; GO_process:
GO:0036267 - invasive filamentous growth [Evidence IGI]
[PMID 9443897]; GO_process: GO:0031382 - mating projection
assembly [Evidence IGI] [PMID 9443897]; GO_process:
GO:2000251 - positive regulation of actin cytoskeleton
reorganization [Evidence IGI,IMP] [PMID 12857882];
GO_process: GO:2000251 - positive regulation of actin
cytoskeleton reorganization [Evidence IMP] [PMID
19633059]; GO_process: GO:0007124 - pseudohyphal growth
[Evidence IMP] [PMID 9055077]; GO_process: GO:0007124 -
pseudohyphal growth [Evidence IMP] [PMID 9443897];
GO_process: GO:0008360 - regulation of cell shape
[Evidence IEA]; GO_process: GO:0031384 - regulation of
initiation of mating projection growth [Evidence IMP]
[PMID 14734532]; GO_process: GO:0032880 - regulation of
protein localization [Evidence IMP] [PMID 10085294];
GO_process: GO:0032880 - regulation of protein
localization [Evidence IMP] [PMID 11740491]; GO_process:
GO:0032880 - regulation of protein localization [Evidence
IMP] [PMID 12857882]; GO_process: GO:0032880 - regulation
of protein localization [Evidence IMP] [PMID 16166638];
GO_process: GO:0032880 - regulation of protein
localization [Evidence IMP] [PMID 8909546]; GO_process:
GO:0032880 - regulation of protein localization [Evidence
IMP] [PMID 9571251]; GO_process: GO:0032880 - regulation
of protein localization [Evidence IMP] [PMID 9632790];
GO_process: GO:0031385 - regulation of termination of
mating projection growth [Evidence IMP] [PMID 14734532]"
/codon_start=1
/product="Spa2p"
/protein_id="XP_018736280.1"
/db_xref="GeneID:30036916"
/translation="MRTLAENQRNLEPAEKMAEQIHALKVEVQEWKSRYSRAKGLVRN
LRNSAYGGNSVFATPDVGLSNDSPYISTEGRVRDLSVTKFQVAVDEFLTKSRLGTTDL
LDNLHNVVVATRHITQDLNGPDDASPVSEKIETELAQCATLVSKTANQLISTTRNHRI
SGGMAPISILDAVTSDLSFAVIELIKVAKVRRTNNRDNRGSASFSPKEIDVSKASRFS
GNSVTSPADMHPAVTPTEDIHMERSDPRRYASTSNQFSTTNFNIEDPDNTISELQGYL
EEQTATAIDSIQSLLTAIKDGSSLYVLRHNMLDIIKLVDITVVATSTSMKQTKNWLLK
DKGSYILENLRDCSQRMAQLQEETKNIDGDSNPDRQVKQRLAGIAFDMAKFTKELVKT
VEEVSLTSEINHIDNQLR"
gene complement(<438011..>439675)
/gene="AMD2"
/locus_tag="AWJ20_4751"
/db_xref="GeneID:30036917"
mRNA complement(<438011..>439675)
/gene="AMD2"
/locus_tag="AWJ20_4751"
/product="Amd2p"
/transcript_id="XM_018881843.1"
/db_xref="GeneID:30036917"
CDS complement(438011..439675)
/gene="AMD2"
/locus_tag="AWJ20_4751"
/inference="similar to AA sequence:KEGG_Orthology:K01426"
/note="Putative amidase; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0004040
- amidase activity [Evidence IEA]; GO_function: GO:0004040
- amidase activity [Evidence ISS] [PMID 2263500];
GO_function: GO:0016884 - carbon-nitrogen ligase activity,
with glutamine as amido-N-donor [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016811 - hydrolase activity, acting
on carbon-nitrogen (but not peptide) bonds, in linear
amides [Evidence IEA]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0008152 -
metabolic process [Evidence IEA,IEA,IEA]"
/codon_start=1
/product="Amd2p"
/protein_id="XP_018736281.1"
/db_xref="GeneID:30036917"
/translation="MVFSYFLHKKTVAAKQKEREDRINDLPEYKEPLTEEEKSILSQE
ASKVIAKVQAGEIKPISVLRAYGKRALESQPELNAITEIFIKDSEKWAEHANTKGPLA
GFPITFKDTINVTGYDSCIGYSSRSFKPAVKDAPIVKLLKDAGAVPMLKTNVPFTLLS
FESYNDVWGVTSNPYHKDYAPGGSTGGEAALLAYGGSRLGVGTDVAGSVRVPAHFSGI
YSLKCSTGRFPKIGNTTSMAGQEGIPAVYSPMTRSLPDLGFFLKTIIDMKPWEYDYSV
HPIPWRKVDLPAKLKVGVIYDDGVVTPSPACARALDLTIDALEKQGHSIVKFDAPDPL
RCLRIASQLLCSDAGRIATRGQLWGENNDAGVGRMLFAQRLPRFVKKIYAWILQYIYR
DPVWAYMVRDWNEKTITERWDLVAEREQYKLEFFEAWKESGIDILLTVPNATPALPHK
GLYESISSCGYTFMFNLLDYSAGVLPVTRVDKEKDQLPPGFNPNKLNRIAKGAYDNYD
ATKMHGLPVGVQVVGQRLEEEKVIKAMEVVEDALHKSGVVYVQRNS"
gene complement(<442508..>442924)
/locus_tag="AWJ20_4752"
/db_xref="GeneID:30036918"
mRNA complement(<442508..>442924)
/locus_tag="AWJ20_4752"
/product="gluconokinase"
/transcript_id="XM_018881844.1"
/db_xref="GeneID:30036918"
CDS complement(442508..442924)
/locus_tag="AWJ20_4752"
/inference="similar to AA sequence:KEGG_Orthology:K00851"
/note="Putative gluconokinase; sequence similarity to
bacterial and human gluconokinase; green fluorescent
protein (GFP)-fusion protein localizes to the cytoplasm;
upregulated by deletion of the RNAP-II associated factor,
PAF1; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0046316 -
gluconokinase activity [Evidence IEA]; GO_function:
GO:0016301 - kinase activity [Evidence IEA,IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0046177 -
D-gluconate catabolic process [Evidence IEA]; GO_process:
GO:0019521 - D-gluconate metabolic process [Evidence IEA];
GO_process: GO:0008150 - biological_process [Evidence ND];
GO_process: GO:0005975 - carbohydrate metabolic process
[Evidence IEA]; GO_process: GO:0016310 - phosphorylation
[Evidence IEA]"
/codon_start=1
/product="gluconokinase"
/protein_id="XP_018736282.1"
/db_xref="GeneID:30036918"
/translation="MSNGIPLTDDDRWDWLADVAATSSRAAAGNESTYIAVAACSALT
PKYREFLKSKVLPGTRIVIAFMWAPEEVLVARVGARKNHYMGTNMVASQAALMQVPKD
EELLENGGFSIPMSTVDQDPTFIAGEVVKKVNAFLK"
gene complement(<443791..>444552)
/gene="MRPL15"
/locus_tag="AWJ20_4753"
/db_xref="GeneID:30036919"
mRNA complement(<443791..>444552)
/gene="MRPL15"
/locus_tag="AWJ20_4753"
/product="mitochondrial 54S ribosomal protein YmL15"
/transcript_id="XM_018881845.1"
/db_xref="GeneID:30036919"
CDS complement(443791..444552)
/gene="MRPL15"
/locus_tag="AWJ20_4753"
/inference="similar to AA sequence:KEGG_Orthology:K17437"
/note="Mitochondrial ribosomal protein of the large
subunit; GO_component: GO:0005762 - mitochondrial large
ribosomal subunit [Evidence IPI] [PMID 12392552];
GO_component: GO:0005762 - mitochondrial large ribosomal
subunit [Evidence IDA] [PMID 9151978]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IEA]; GO_function:
GO:0004525 - ribonuclease III activity [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IPI] [PMID 12392552]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IDA] [PMID 9151978]; GO_process: GO:0090501 - RNA
phosphodiester bond hydrolysis [Evidence IEA]; GO_process:
GO:0090502 - RNA phosphodiester bond hydrolysis,
endonucleolytic [Evidence IEA]; GO_process: GO:0006396 -
RNA processing [Evidence IEA]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IC] [PMID 12392552];
GO_process: GO:0032543 - mitochondrial translation
[Evidence IC] [PMID 9151978]"
/codon_start=1
/product="mitochondrial 54S ribosomal protein YmL15"
/protein_id="XP_018736283.1"
/db_xref="GeneID:30036919"
/translation="MYLRQIPRSLRPFQPLGRLVVGESAGISHGQLAFKSQQTQQVRT
LVYTKDGVVRARGLKKNEEEYLTVNGVTYPGNEDTLKDAKTFLGEEYSLPDPVILQTI
THKSFAHGKKPYNEKLAILGKEFLRLQASHYAVSQTNDNPHAINKHNFDISPQPIEVL
SSSVALHEVCKKTGIHKNIFWKKRISENTSTGQELDAKFTGEQSVFARSIHALIGAIL
LRHGEPKARKFISEKLLSGPYSLISVSEKVYAPRP"
gene <445059..>446798
/gene="ROK1"
/locus_tag="AWJ20_4754"
/db_xref="GeneID:30036920"
mRNA <445059..>446798
/gene="ROK1"
/locus_tag="AWJ20_4754"
/product="RNA-dependent ATPase ROK1"
/transcript_id="XM_018881846.1"
/db_xref="GeneID:30036920"
CDS 445059..446798
/gene="ROK1"
/locus_tag="AWJ20_4754"
/inference="similar to AA sequence:KEGG_Orthology:K14779"
/note="RNA-dependent ATPase; involved in pre-rRNA
processing at sites A0, A1, and A2, and in control of cell
cycle progression; contains two upstream open reading
frames (uORFs) in 5' untranslated region which regulate
translation; GO_component: GO:0030686 - 90S preribosome
[Evidence IDA] [PMID 12150911]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 9154839]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0004004 - ATP-dependent RNA helicase activity [Evidence
IDA] [PMID 23153376]; GO_function: GO:0008026 -
ATP-dependent helicase activity [Evidence IEA];
GO_function: GO:0003723 - RNA binding [Evidence IEA];
GO_function: GO:0008186 - RNA-dependent ATPase activity
[Evidence IDA] [PMID 18975973]; GO_function: GO:0008186 -
RNA-dependent ATPase activity [Evidence IDA] [PMID
23153376]; GO_function: GO:0004386 - helicase activity
[Evidence IEA,IEA]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0003676 - nucleic
acid binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006200
- ATP catabolic process [Evidence IEA]; GO_process:
GO:0000480 - endonucleolytic cleavage in 5'-ETS of
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 9154839]; GO_process:
GO:0000447 - endonucleolytic cleavage in ITS1 to separate
SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic
rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 9154839]; GO_process: GO:0000472 -
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 9154839]; GO_process: GO:0006364 - rRNA
processing [Evidence IEA]; GO_process: GO:0042254 -
ribosome biogenesis [Evidence IEA]; GO_process: GO:0048254
- snoRNA localization [Evidence IMP] [PMID 18833290]"
/codon_start=1
/product="RNA-dependent ATPase ROK1"
/protein_id="XP_018736284.1"
/db_xref="GeneID:30036920"
/translation="MDIFKLLSRGATIQRNGKHQKDLELFNPNATAAKEVAAGSDSVD
LKIAKEIDFFKNASKADKKAKKVSSETNNKDKKVKQSNKNVIDNRNDEDDEEDDAVSV
PLSITSPEEASVQRKKFKIKTTGDDIPLPIGSFEDLTTRFQLHRHLLRNLEQQNFSSP
TAIQSESIPILLHGRDLIACAPTGSGKTLAFSIPLVQSLKEHKTTGIRCLIVSPTKEL
ATQIYNEVVKLSRGRDLNICILTKAAAAKFRNDALSRQKFDILITTPLRLVDAVKSEI
IDLSHVQHLVLDEADKLFEQGFLEQTDDILAACSNPRLQKALFSATIPSGVEEMAKTI
MHSPIRIIVGHKEGASHDIDQKLVYAGNEQGKLIAIRQMITGGEVVPPVIIFVQSIQR
AKALFHELLYDKVNVDAIHGERTQNQRDKVIERFKQGEIWVLICTDVLSRGIDFRGVN
LVINYDVPQSSQSYVHRIGRTGRAGRAGKAVTFFTKDDHDAVKNVVNVMKQSGCDVGD
WMLKMKKITKNDKKQLKKKPVERTEISTVPSVIRQKRKQRRDMVEASKKRKLHSTDDG
PASAEQSSDEASD"
gene complement(<447044..>447526)
/gene="ATP20"
/locus_tag="AWJ20_4755"
/db_xref="GeneID:30036921"
mRNA complement(<447044..>447526)
/gene="ATP20"
/locus_tag="AWJ20_4755"
/product="F1F0 ATP synthase subunit g"
/transcript_id="XM_018881847.1"
/db_xref="GeneID:30036921"
CDS complement(447044..447526)
/gene="ATP20"
/locus_tag="AWJ20_4755"
/inference="similar to AA sequence:KEGG_Orthology:K02140"
/note="Subunit g of the mitochondrial F1F0 ATP synthase;
reversibly phosphorylated on two residues;
unphosphorylated form is required for dimerization of the
ATP synthase complex, which in turn determines
oligomerization of the complex and the shape of inner
membrane cristae; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0031966 - mitochondrial
membrane [Evidence IEA]; GO_component: GO:0000276 -
mitochondrial proton-transporting ATP synthase complex,
coupling factor F(o) [Evidence IEA]; GO_component:
GO:0000276 - mitochondrial proton-transporting ATP
synthase complex, coupling factor F(o) [Evidence
IMP,IPI,ISS] [PMID 10336613]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0045263 - proton-transporting ATP
synthase complex, coupling factor F(o) [Evidence IEA];
GO_function: GO:0015078 - hydrogen ion transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0046933 - proton-transporting ATP synthase activity,
rotational mechanism [Evidence IDA] [PMID 20691145];
GO_function: GO:0005198 - structural molecule activity
[Evidence IMP,IPI] [PMID 9857174]; GO_process: GO:0006754
- ATP biosynthetic process [Evidence IEA]; GO_process:
GO:0015986 - ATP synthesis coupled proton transport
[Evidence IEA]; GO_process: GO:0015986 - ATP synthesis
coupled proton transport [Evidence IMP,ISS] [PMID
10336613]; GO_process: GO:0015986 - ATP synthesis coupled
proton transport [Evidence IDA] [PMID 20691145];
GO_process: GO:0015986 - ATP synthesis coupled proton
transport [Evidence IMP,IPI] [PMID 9857174]; GO_process:
GO:0042407 - cristae formation [Evidence IMP] [PMID
19528297]; GO_process: GO:0006811 - ion transport
[Evidence IEA]; GO_process: GO:0006461 - protein complex
assembly [Evidence IMP] [PMID 17761666]; GO_process:
GO:0035786 - protein complex oligomerization [Evidence
IMP] [PMID 16551625]; GO_process: GO:0015992 - proton
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="F1F0 ATP synthase subunit g"
/protein_id="XP_018736285.1"
/db_xref="GeneID:30036921"
/translation="MLRNQLLNNSRLIARRSVRYNSTASQASSAVSKASSAVSTITSK
AAGFVNCSIYWAKVVAELGKHIYVKEGLSPPSGTQFQSVYKNLWQQILTQSKHFANSP
AQLVDTVTGVSRQQVVAGGAYLVQFAGLFALGEIIGRRNVFGYPHHHVEEEAEHAAEH
"
gene <448978..>450198
/locus_tag="AWJ20_4756"
/db_xref="GeneID:30036922"
mRNA <448978..>450198
/locus_tag="AWJ20_4756"
/product="hypothetical protein"
/transcript_id="XM_018881848.1"
/db_xref="GeneID:30036922"
CDS 448978..450198
/locus_tag="AWJ20_4756"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736286.1"
/db_xref="GeneID:30036922"
/translation="MTFQVDPIKDAQYNIHPFTHVHETSDKTLDYVDLVAIDLSKYTD
TPEGQESRRALAKQLHNALSTYGFFYVTGHGIEKERIDHLYSVAQSILELPDEIKYKH
LAGALRSDDEDRSVSLGAERGGGFKPRQYWAMKNGVRDQIEHYNFLDLLHDKLMTEQN
HPEIAKAYLEEVAEYYRLLHHTVLKKITVLCDIILEIPEGTIWEKNFKVVPEDRDASG
SGFGRFMMYHGMSPEDEAKTDDTWLRGHSDSTAFTFITSQPMASLQIRDYYTGKWRYI
PYLPNAFVVNIGDAMEFITGGYFKSTIHRVVKPPADQLYNRRLGLIYFSKPSAKSVID
PEAVNSPLLERLGYTKPEEWDRIEYAKWDDEKGRLFGRKDLNNTQTDEPLPVKLYGRY
AERWHQASGPKVFT"
gene <450577..>452274
/gene="FMO1"
/locus_tag="AWJ20_4757"
/db_xref="GeneID:30036923"
mRNA <450577..>452274
/gene="FMO1"
/locus_tag="AWJ20_4757"
/product="Fmo1p"
/transcript_id="XM_018881849.1"
/db_xref="GeneID:30036923"
CDS 450577..452274
/gene="FMO1"
/locus_tag="AWJ20_4757"
/note="Flavin-containing monooxygenase; localized to the
cytoplasmic face of the ER membrane; catalyzes oxidation
of biological thiols to maintain the ER redox buffer ratio
for correct folding of disulfide-bonded proteins;
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IDA] [PMID 10077572]; GO_function: GO:0004499 -
N,N-dimethylaniline monooxygenase activity [Evidence IEA];
GO_function: GO:0004499 - N,N-dimethylaniline
monooxygenase activity [Evidence IDA] [PMID 10600176];
GO_function: GO:0050661 - NADP binding [Evidence IEA];
GO_function: GO:0050660 - flavin adenine dinucleotide
binding [Evidence IEA]; GO_function: GO:0004497 -
monooxygenase activity [Evidence IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]; GO_process: GO:0006457 - protein
folding [Evidence IDA] [PMID 10077572]"
/codon_start=1
/product="Fmo1p"
/protein_id="XP_018736287.1"
/db_xref="GeneID:30036923"
/translation="MNVKRIAIIGAGPAGAAGLDSFLSHGFTDIRVFERRPLPGGTWV
GDTELGFASNTGNTVPPIDDEVGDVPVEIPPEAASATEESPYVRKATIKARRFDEPTL
YSQLETNILPELMSFKRRSMPKNLLSNSVSKYENEDVFRSYKTIQEYVGEFFTGENEK
YVSYNTSVEQAEQPDGPGTKWVLTLKRQNGQNNNEEWWTEEFDGVYAASGRYNVPRVS
HINGLDEVAKAVPQGVIEHTKWFRGAESYKGKKVLIVGASVSTTDIIQLVKETAQLPL
YLSVRHVAPAYRAGFEQPFVDGRGSITSVSLNESGKSIDVIFEDGSTVTNIEKLVLAT
GYNFSYPYLEKYTQKFGGIVRNNVLHNVFLTTWWSEDPTLVLPGVFTEGINFRCMETQ
AMATAALWSGFPGSGFPSKEICVQWEADRRKFKTPAQWHFWFPHFDNIIEAIAKVGGG
YKALCEFAGITSSDLKTFVPVYEKGIAFKCLFFTKVANEYMLKHPKTLQQYNQTVALL
KTKTVEPDPTAAEADHELFKRLFVNWSGGRGEPKIPSKIQTTELSEKTPVVTEVAVK"
gene <452728..>454086
/gene="DUS1"
/locus_tag="AWJ20_4758"
/db_xref="GeneID:30036924"
mRNA <452728..>454086
/gene="DUS1"
/locus_tag="AWJ20_4758"
/product="Dus1p"
/transcript_id="XM_018881850.1"
/db_xref="GeneID:30036924"
CDS 452728..454086
/gene="DUS1"
/locus_tag="AWJ20_4758"
/inference="similar to AA sequence:KEGG_Orthology:K05542"
/note="Dihydrouridine synthase; member of a widespread
family of conserved proteins including Smm1p, Dus3p, and
Dus4p; modifies pre-tRNA(Phe) at U17; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003824 - catalytic activity [Evidence
IEA]; GO_function: GO:0050660 - flavin adenine
dinucleotide binding [Evidence IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA];
GO_function: GO:0017150 - tRNA dihydrouridine synthase
activity [Evidence IEA]; GO_function: GO:0017150 - tRNA
dihydrouridine synthase activity [Evidence IDA] [PMID
12003496]; GO_function: GO:0017150 - tRNA dihydrouridine
synthase activity [Evidence IMP] [PMID 14970222];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]; GO_process: GO:0002943 - tRNA
dihydrouridine synthesis [Evidence IEA]; GO_process:
GO:0006400 - tRNA modification [Evidence IDA] [PMID
12003496]; GO_process: GO:0006400 - tRNA modification
[Evidence IMP] [PMID 14970222]; GO_process: GO:0008033 -
tRNA processing [Evidence IEA,IEA]"
/codon_start=1
/product="Dus1p"
/protein_id="XP_018736288.1"
/db_xref="GeneID:30036924"
/translation="MTVSQAATPEPEVVTKTAAPVKTVVERPSEFTVENKLTGRDLLK
SVGFPKTIVAPMVDQSELAWRLISRKYGADLCYTPMFHARLFGEDKRYREDQFGPLDG
DANVDRPLIVQFCANDPEELLKAAKYVVGRCDAVDLNLGCPQGIAKKGKYGAFLMEDW
DLIYRLINILHTKLEIPVTAKIRIYETKEKTLEYAKMVLSAGAQFLTVHGRTREMKGQ
LTGLADWSYIRYVRDNLPPDTVIFANGNVLYSEDIDRSLEATKADAVMSAESNLYNPG
VFNVNKPGIDDQFPRVDKLLREYFETAKSTPGNASQTSMKSHFFKVLQSFLPKHLNIR
NAIGPIRKGQYDQFEDVVKQVEEIVEGIYEKVNKGELEDKVITLPDGYKSVPYWRCQP
YFRVVNGVLSNGKRKADVDPEAAQRKKEAKLEKEKAAAALASLSSDGPQPTGDEAPEP
SN"
gene complement(<455674..>456630)
/gene="cwf15"
/locus_tag="AWJ20_4759"
/db_xref="GeneID:30036925"
mRNA complement(<455674..>456630)
/gene="cwf15"
/locus_tag="AWJ20_4759"
/product="complexed with Cdc5 protein Cwf15"
/transcript_id="XM_018881851.1"
/db_xref="GeneID:30036925"
CDS complement(455674..456630)
/gene="cwf15"
/locus_tag="AWJ20_4759"
/inference="similar to AA sequence:KEGG_Orthology:K12863"
/codon_start=1
/product="complexed with Cdc5 protein Cwf15"
/protein_id="XP_018736289.1"
/db_xref="GeneID:30036925"
/translation="MTTAHRPTFDPARGQKNTQISGSIVHTRMLPAHTQLKFRKRGQG
GAADADAHGTDGGKRDFKRELLLAENESKQSNGKKRGLVEYDAEEEEAADVGSSRKRR
EISDGSVSKSVPLLKGDETSNSLHNGDEEKNNENIPEDESDSDVGDTDGYDSKARNED
EDEEYRRRILEKYKDIDADSDSDGDDDDEEKDEDDDDDEDEDDDDDDDDEEDETVMLM
RELEKIKKEKALEKQRLEEIAARKAEEEREREIAYGNPLMNPATKDGKDSFAVKRSWM
EDTVFKNQASSLAANQSEPKKELINDLLRSDFHKKFMDRYIK"
gene <459024..>460070
/gene="NBP2"
/locus_tag="AWJ20_4760"
/db_xref="GeneID:30036927"
mRNA <459024..>460070
/gene="NBP2"
/locus_tag="AWJ20_4760"
/product="Nbp2p"
/transcript_id="XM_018881853.1"
/db_xref="GeneID:30036927"
CDS 459024..460070
/gene="NBP2"
/locus_tag="AWJ20_4760"
/note="Protein involved in the HOG (high osmolarity
glycerol) pathway; negatively regulates Hog1p by
recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex;
interacts with Bck1p and down regulates the cell wall
integrity pathway; found in the nucleus and cytoplasm,
contains an SH3 domain and a Ptc1p binding domain (PBM);
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14573466]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 11914276]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0006972 -
hyperosmotic response [Evidence IMP,IPI] [PMID 14685261];
GO_process: GO:0000200 - inactivation of MAPK activity
involved in cell wall organization or biogenesis [Evidence
IMP,IPI] [PMID 22570491]; GO_process: GO:0006469 -
negative regulation of protein kinase activity [Evidence
IDA] [PMID 14685261]; GO_process: GO:0009408 - response to
heat [Evidence IMP] [PMID 14573466]"
/codon_start=1
/product="Nbp2p"
/protein_id="XP_018736290.1"
/db_xref="GeneID:30036927"
/translation="MAAKPPSPIASSAFQSGASHDISVLQDVLSGVSQPHDSKTDVVS
ENLDSKHQQLEHTDNDSPENNSDVAHQNVDAKGSVVYQDLEEIPNFHSALSYATVRDF
AYPEFHPLHYGVPEFEQASGETDDDEDYEPTYEYNDTYDANDPHSHYYNDKSAGFEDG
PPWQQDSDLNSPVIKSHEVGDRISKEYQFSVASADEIHGRAVALFDFTPENDNEAPLQ
EGQIVWISYRHGQGWLVAEDPETGETGLVPEEYVQLLNSISYEEDILQDSSEPAIYQS
PESDSEGWVDEAEPEGDAAEPATEPVVKSASDTDYTDLKKGIKDLSLRESEEHEHNQT
EPETAEVYSQVPSE"
gene complement(<460379..>461458)
/locus_tag="AWJ20_4761"
/db_xref="GeneID:30036928"
mRNA complement(<460379..>461458)
/locus_tag="AWJ20_4761"
/product="hypothetical protein"
/transcript_id="XM_018881854.1"
/db_xref="GeneID:30036928"
CDS complement(460379..461458)
/locus_tag="AWJ20_4761"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736291.1"
/db_xref="GeneID:30036928"
/translation="MDTVQQARKLLESSEAAKCLELALPVLDSQSSDDVKLEALHLIG
EAYLENGDPKNAFECFSKCAELDPDGSKGGMEKFLWLGQLSGGEPGLKWFEKGLHGLR
NALTEVNNKAASSSKVNGDNDLQMQFQVKLLHKKINEALCGMIEVWMTDLCMEPEAES
QCEKLIAEALLLDEEIPESWSMLGSIRISQQRNDDAREALERSWQLFQAVGDGSIDEL
PSLIRLAQNMMEMGQHQYVLDVTKRIHVMDDQVVDSFYLNGLAHNELYKEIRDGPDAE
SKSRIAARHIAGARDAWTLLLNLTNTGDVEVDDELVSSVQELLTTLPEVGDDDFSSSS
EEEDEDMEDDDDKVDLKDLDSDEEL"
gene complement(<461752..>464247)
/gene="RLF2"
/locus_tag="AWJ20_4762"
/db_xref="GeneID:30036929"
mRNA complement(<461752..>464247)
/gene="RLF2"
/locus_tag="AWJ20_4762"
/product="Rlf2p"
/transcript_id="XM_018881855.1"
/db_xref="GeneID:30036929"
CDS complement(461752..464247)
/gene="RLF2"
/locus_tag="AWJ20_4762"
/note="Largest subunit (p90) of the Chromatin Assembly
Complex (CAF-1); chromatin assembly by CAF-1 is important
for multiple processes including silencing at telomeres,
mating type loci, and rDNA; maintenance of kinetochore
structure; deactivation of the DNA damage checkpoint after
DNA repair; and chromatin dynamics during transcription;
GO_component: GO:0033186 - CAF-1 complex [Evidence IDA]
[PMID 9030687]; GO_component: GO:0000775 - chromosome,
centromeric region [Evidence IDA] [PMID 11782447];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
9030688]; GO_function: GO:0042393 - histone binding
[Evidence IDA] [PMID 16503640]; GO_process: GO:0006335 -
DNA replication-dependent nucleosome assembly [Evidence
IDA] [PMID 9030687]; GO_process: GO:0006335 - DNA
replication-dependent nucleosome assembly [Evidence IMP]
[PMID 9030688]"
/codon_start=1
/product="Rlf2p"
/protein_id="XP_018736292.1"
/db_xref="GeneID:30036929"
/translation="MLSLQAKTGPMSPKDTNIVSGKSIDNATPRDPVETVKTKLCKRD
RDNDIGQGQENSGIPIKNSQSKNQDSSLSNIESNTHERSSKDKTVKAKESSTTSAPDT
SKQSELPSQKTNPSVTQSDSPVNWTGANQNIVMMPNNVPMNVQGMTPNMVPPPNMIFT
NMLASGMIPPNMIPPGMVPANMISPNAIPPTTIPLNMIPPQMIPPHMLPPNMVSPPFL
PSGMIPVSTIQDGESTPSPERPNKKQKIGKTKEEREQERLDKENERLAKLEAEQQKKA
EKMAEKMAEKQRREEEKEEKKRQKEEEKRLKEEEKRLKEAAKEEEKRQKEEEKRLKEA
AKEEERRQKEEEKRLKEEAKEQARLKEERKQLRMDRFFKKVQKPTSAETPAGSSESKE
ARSFSSSIIDDSNNSSLNATKSTASESTASVESSSNGSENSTEESKADLNTSPCTFDE
IFLPFHIKVDTTLCARDSWIEKRARNELPSTDIDIFLKTNKQQRGFSPDKTTVEIIQL
MNTGSLTESRVMELLNKLPRRHLKFAENIRPPYVGTFSKPTPRIMVIQPWKRFIAPQD
TNPETLVSTTDLIPDTTRVELGNSLLSINYDYDSDYDWVNDNEEGDEEIGEDIGEEDE
SESDDDDNEMAGFVEDESDTPTPSSSSDEGSPTNTNRRRIIGPLASLVAWNDGTEPHI
FDHMETALLIDLKGDASIDPFKDYWASSPAEATAVGTALPSTASPTSSATATPAKLIP
DHLLRDFLTKIQGSDKNQTLLVETLKIEFNSVSKSTIRDTIKACAKRVGAKENDKRWV
INPPFADKYGILTAPTTNSTSTTRQASPFLAGN"
gene complement(<465594..>468080)
/gene="VPS70"
/locus_tag="AWJ20_4763"
/db_xref="GeneID:30036930"
mRNA complement(<465594..>468080)
/gene="VPS70"
/locus_tag="AWJ20_4763"
/product="Vps70p"
/transcript_id="XM_018881856.1"
/db_xref="GeneID:30036930"
CDS complement(465594..468080)
/gene="VPS70"
/locus_tag="AWJ20_4763"
/inference="similar to AA sequence:KEGG_Orthology:K01301"
/note="hypothetical protein involved in vacuolar protein
sorting; GO_component: GO:0005575 - cellular_component
[Evidence ND]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_function:
GO:0004177 - aminopeptidase activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0008237 - metallopeptidase
activity [Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0008233
- peptidase activity [Evidence IEA,IEA]; GO_process:
GO:0006623 - protein targeting to vacuole [Evidence IMP]
[PMID 12134085]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0006508 -
proteolysis [Evidence IEA,IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Vps70p"
/protein_id="XP_018736293.1"
/db_xref="GeneID:30036930"
/translation="MVVVRVDEESQSLIRGPNGLKVRKRRASCLRFGCYTLLGVFFLA
VFNVIFLPRTSISRDWNRLHSIKVSDADVKRTLFQSVNASSIRDWSYKYTQEPHLAGT
NYGLVEWTRDKFEEYGIPAEIVSYDIYLNYPRDHAVKLLNEDGSVQFSASLEEAVIEE
DPTTSREDRVPTFHGYSASGNVTGQLIYVNYGTREDYDQLIAKGVDFKGKIVIARYGG
IFRGLKVKFAQDLGAIGALLYSDPGDDGGITIKNGYEAYPNGPARHPSSVQRGSVQFL
SLGPGDPTTPGWASKPDSKRVDPYDTIPSIPSIPISYEDALPLLKAINGKGLKGSDLG
ENWVGELTDEFDYSVGPSTLEVNLYNDQDYDIRPTYNVIGKIEGIISDEAIVIGNHRD
AWIAGGAGDPNSGSAALIELAKAFGELKKIGWKPSRTIILGSWDGEEYGLLGSTEWGE
DNAEYLQHHVIAYLNVDVAFSGSRFGASASPLLDNVLIDVTKQVPAPSGNGTLYDLWY
SQNGARISSLGSGSDYTVFQDYLGIPSVDFGFGGGKGDPVYQYHSNYDSFYWMDKFGD
KDFAHHSVMVKAWGLLTLTLSETELIPFKLASYAKLLDTYLEKTESQLPKEIFDDGDD
EYDDDDDFDFDFSEEMSVDMAVAIANADSSALASVNPVSSTDVSDTEVDAQQIFTRPK
HRKPKHPKRKLHKEARKAFEKLRKEVDKFKTRARKYDAYTERLQEEFTQDYAWFYSYK
KLILLVKIKVANIKLAQIEQLFTYDKGLDNGRSWFKHVVFAPGINTGYAGALLPGLTE
AIDARDPSAILKWATIIRASVTSVNALVSK"
gene complement(<468452..>471052)
/gene="slx4"
/locus_tag="AWJ20_4764"
/db_xref="GeneID:30036931"
mRNA complement(<468452..>471052)
/gene="slx4"
/locus_tag="AWJ20_4764"
/product="structure-specific endonuclease subunit"
/transcript_id="XM_018881857.1"
/db_xref="GeneID:30036931"
CDS complement(468452..471052)
/gene="slx4"
/locus_tag="AWJ20_4764"
/codon_start=1
/product="structure-specific endonuclease subunit"
/protein_id="XP_018736294.1"
/db_xref="GeneID:30036931"
/translation="MEDVVLVSSTGIDTSSPNSLSNNSPVHSRLTRTISKFAFEGSVS
NITTTTTTTTKTGSFVSSAVELETLDAQHVTITTTVSKELTVNHSPQASKEKKKKRKK
SATTKSSETSKKSVVKKTRKPKEKVPATLNERMVAIFEKSALITVPDEYRGNVDPSHH
GIDITRNDSNGYYINNNDRSENNDNKDENKNSYYNETNDKSQQDDRMAASISSTIARH
DISANNRPFNFGCDIQLNDPDYDNARLLSGKPGKVRALRRNRQNQEKQDRASGQSKRL
KPSYQLEAVPKPQIPADFLVSVEEGCQNIKQLKLIFPDTPKPKRHCSSQRNNSAETIS
PVRNRTSFNREKRGSESLTMFDLASRIPSDILSQEMRMLDSRSEKENIFDEEVPEAGD
TDSDESPEIAVSTSSKLAAVSVLTPNRSLVSGSGKSITNSRAEFDDGVQVLTKNVHPR
RVLTPLLNSEVRSQSDPFVASRSGDDRTSQNAISLPRSKRQDSNTEKNSFVEIYEISD
SENDYGSPLVLSQQRPTNLLCNSPIRQLSAATIITISSSPIHNETGPSKPDKLNSIDT
FDEFHSSSPPRASLSISSARRLTLEPDKISDRLANSTDDSYSFNSVDEVPQSPGYADS
ENLLEYAPYIMTVGEPYKDIISGSPPNRCSQPISIPSSSISTFSSFGSQDDTANSNVP
ITDIHSEPQTTDFVDPTPLTDKTADELPPRTENVDYKSIYEAMPLDELRRTVDKYGFK
PRRSRQAMIELLLSCHTDTTDIPSIAAVSQPSTSMPNSDSDMRSKLFTRISTQIRTNP
LAKPWWTKMLLYEPLPLESFQRFLQQTLSIDLDLELVRSWCDHCGVCTRASDPTHSDT
PSSQTSETEV"
gene complement(<471658..>472089)
/gene="CPR1"
/locus_tag="AWJ20_4765"
/db_xref="GeneID:30036932"
mRNA complement(<471658..>472089)
/gene="CPR1"
/locus_tag="AWJ20_4765"
/product="peptidylprolyl isomerase CPR1"
/transcript_id="XM_018881858.1"
/db_xref="GeneID:30036932"
CDS complement(471658..472089)
/gene="CPR1"
/locus_tag="AWJ20_4765"
/inference="similar to AA sequence:KEGG_Orthology:K01802"
/note="Cytoplasmic peptidyl-prolyl cis-trans isomerase
(cyclophilin); catalyzes the cis-trans isomerization of
peptide bonds N-terminal to proline residues; binds the
drug cyclosporin A; N-terminally propionylated in vivo;
protein abundance increases in response to DNA replication
stress; GO_component: GO:0034967 - Set3 complex [Evidence
IDA] [PMID 11711434]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0000118 - histone
deacetylase complex [Evidence IPI] [PMID 15643056];
GO_component: GO:0005758 - mitochondrial intermembrane
space [Evidence IEA]; GO_component: GO:0005758 -
mitochondrial intermembrane space [Evidence IDA] [PMID
22984289]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 15643056]; GO_function:
GO:0016018 - cyclosporin A binding [Evidence IMP] [PMID
7515500]; GO_function: GO:0016853 - isomerase activity
[Evidence IEA]; GO_function: GO:0003729 - mRNA binding
[Evidence IDA] [PMID 23222640]; GO_function: GO:0042277 -
peptide binding [Evidence IEA]; GO_function: GO:0003755 -
peptidyl-prolyl cis-trans isomerase activity [Evidence
IEA,IEA,IEA]; GO_function: GO:0003755 - peptidyl-prolyl
cis-trans isomerase activity [Evidence IDA] [PMID
2687115]; GO_process: GO:0030437 - ascospore formation
[Evidence IMP] [PMID 15643056]; GO_process: GO:0044267 -
cellular protein metabolic process [Evidence IMP] [PMID
11641409]; GO_process: GO:0044267 - cellular protein
metabolic process [Evidence IMP] [PMID 12242280];
GO_process: GO:0016575 - histone deacetylation [Evidence
IDA] [PMID 11711434]; GO_process: GO:0045836 - positive
regulation of meiosis [Evidence IGI,IMP] [PMID 15643056];
GO_process: GO:0006457 - protein folding [Evidence
IEA,IEA]; GO_process: GO:0000413 - protein peptidyl-prolyl
isomerization [Evidence IEA,IEA,IEA]; GO_process:
GO:0015031 - protein transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="peptidylprolyl isomerase CPR1"
/protein_id="XP_018736295.1"
/db_xref="GeneID:30036932"
/translation="MLTVAQIVPKTAKNFRELATGSEGFGYAKSGFHRVIPEFMLQGG
DFTNHNGTGGKSIYGAKFADENFQAKHTRPGLLSMANAGPNTNGSQFFITTVPCPWLD
GKHVVFGEVVEGYDVVKKIETFGSSSGATRAKLVIDASGTV"
gene <477114..>478451
/gene="ENT3"
/locus_tag="AWJ20_4766"
/db_xref="GeneID:30036933"
mRNA <477114..>478451
/gene="ENT3"
/locus_tag="AWJ20_4766"
/product="Ent3p"
/transcript_id="XM_018881859.1"
/db_xref="GeneID:30036933"
CDS 477114..478451
/gene="ENT3"
/locus_tag="AWJ20_4766"
/inference="similar to AA sequence:KEGG_Orthology:K12471"
/note="Protein containing an N-terminal epsin-like domain;
involved in clathrin recruitment and traffic between the
Golgi and endosomes; associates with the clathrin adaptor
Gga2p; GO_component: GO:0005794 - Golgi apparatus
[Evidence IEA,IEA]; GO_component: GO:0030479 - actin
cortical patch [Evidence TAS] [PMID 10652251];
GO_component: GO:0030125 - clathrin vesicle coat [Evidence
IDA] [PMID 12483220]; GO_component: GO:0030665 -
clathrin-coated vesicle membrane [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0031410 - cytoplasmic vesicle [Evidence
IEA]; GO_component: GO:0005768 - endosome [Evidence IDA]
[PMID 12967568]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_function: GO:0030276 - clathrin binding
[Evidence IDA] [PMID 12967568]; GO_function: GO:0008289 -
lipid binding [Evidence IEA]; GO_function: GO:0080025 -
phosphatidylinositol-3,5-bisphosphate binding [Evidence
IDA] [PMID 14630930]; GO_function: GO:0032266 -
phosphatidylinositol-3-phosphate binding [Evidence IDA]
[PMID 14630930]; GO_function: GO:0005515 - protein binding
[Evidence IPI] [PMID 12483220]; GO_process: GO:0006895 -
Golgi to endosome transport [Evidence IMP] [PMID
12483220]; GO_process: GO:0000147 - actin cortical patch
assembly [Evidence ISS] [PMID 10449404]; GO_process:
GO:0030036 - actin cytoskeleton organization [Evidence
IMP] [PMID 12967568]; GO_process: GO:0007015 - actin
filament organization [Evidence ISS] [PMID 10449404];
GO_process: GO:0034498 - early endosome to Golgi transport
[Evidence IGI] [PMID 20658963]; GO_process: GO:0032511 -
late endosome to vacuole transport via multivesicular body
sorting pathway [Evidence IMP] [PMID 15107463];
GO_process: GO:0071985 - multivesicular body sorting
pathway [Evidence IMP] [PMID 12967568]; GO_process:
GO:0015031 - protein transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Ent3p"
/protein_id="XP_018736296.1"
/db_xref="GeneID:30036933"
/translation="MPMIYKRFTDKSAEEWRQIYKALQLLEFLVKNGSERVVDYARSH
SAVIDMLKHFHFIDQNGKDQGINIRNRAKELTALLNDVERIRTERKKARSNKAKYSGI
GNDIEGDEDFGGFGGTGKKYGGFGSDSMEFGGGYNANKVYGDGGGFNGSNYDGPGYSR
GAGAGDDDEFEEYEVGGGYNDDSSGSAGSTSGSRNKGKTAASSSSRTKTTSSSSAAAP
APVADFISFDDDVAPPAATGASSSAAAEDDDDFDDFQSATPVAGASSNTKTTGGGSST
KADLLDLFSQPSTASSFQSQPLQQQQQFSSFTSSPGLPTQLSSTPSVVSPSAFSTTST
IGSNSTGFPGFSSTPSTPSIGAGSGLGNSSTGKPAAKKANDAFGDLWSSASKTKKSSA
KTEPTSLAALAHENAQKSMWGGAPASSSSPSQPQPQSQQPSSSSGQIDDLLSF"
gene complement(<478679..>479569)
/gene="GIR2"
/locus_tag="AWJ20_4767"
/db_xref="GeneID:30036934"
mRNA complement(join(<478679..479334,479500..>479569))
/gene="GIR2"
/locus_tag="AWJ20_4767"
/product="Gir2p"
/transcript_id="XM_018881860.1"
/db_xref="GeneID:30036934"
CDS complement(join(478679..479334,479500..479569))
/gene="GIR2"
/locus_tag="AWJ20_4767"
/note="Highly-acidic RWD domain-containing hypothetical
protein; cytoplasmic; forms a complex with Rbg2p;
interacts with Rbg1p and Gcn1p; associates with
translating ribosomes; putative intrinsically unstructured
protein; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005844 - polysome
[Evidence IDA] [PMID 19448108]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0002181 -
cytoplasmic translation [Evidence IGI] [PMID 21076151]"
/codon_start=1
/product="Gir2p"
/protein_id="XP_018736297.1"
/db_xref="GeneID:30036934"
/translation="MDNKEEQEQEIDVLRSIYPDELEILSENKYNIHIKLDTTPTKFL
ILEVEYPDDYPQVVPVLDVDNSDGSYVNASGEDDEGYDGSDEEEYEDGIDETQGDSIE
FTNADLVDLRKVLEENAEENIGLPSIFTLASLLKDQAEELVEKKIKIKEKQREKLIFE
QEEKEQAKFRGTPVTPESFKAWRLKFRAELGLDNKEDANKPKHKLTGKEIFEKGLNKD
EDEDDDTPAEPDQLASSVENLAV"
gene <480189..>482804
/gene="MIF2"
/locus_tag="AWJ20_4768"
/db_xref="GeneID:30036935"
mRNA <480189..>482804
/gene="MIF2"
/locus_tag="AWJ20_4768"
/product="Muscle M-line assembly protein unc-89"
/transcript_id="XM_018881861.1"
/db_xref="GeneID:30036935"
CDS 480189..482804
/gene="MIF2"
/locus_tag="AWJ20_4768"
/codon_start=1
/product="Muscle M-line assembly protein unc-89"
/protein_id="XP_018736298.1"
/db_xref="GeneID:30036935"
/translation="MIRLQVRIIPASQADNEVSSRVSRPSNFGPSSYHLQSSTPNASV
KSFSNNDDDTYGFLHLVTPSELLKTVRDSIVNRFAKIYPEEGPLFIERLQDSSGLDLD
PDYKVGDVFADRDDLFVILQEPVTRNDILQSNVSVADEPSLKRRAEDDSNSSIVLRPT
KSRKRKSSIWMTPSNNAADISSLSHTPVPVLSSGTPASHLNVAPQLSESNGSPRVRRR
PSLDLMAKPSAAEIPDSDVDEEAHEGNLSLPPPSNEAYANADPLKPVSPSANLNGSKN
ILNLESDKVQLLPPTPAKKSPIAGKKRAPQSELQLTPEITVKAAEQAEAVLEVASSQP
SQDEASITQESDDKIETGLQPIAGTPTGSQPKAKRQRAKATEKKIQDTLINATETKGV
EVSPISGLTRKEQAQEAKRLREEAKKLKQLEAQKKKEEQLKKKELQAQRKRELEAQKS
AKAAELRELQRKKALEELEKLGEENRRKREEERLKQQEQQQREQKDETNPSQDDIIET
ENSDDGAKGKKRPSAETLRRNQQRLEANRKSMEKFHNAVNSLEKDADGSAAVPEASVE
PPTQSEEPPKRKRGRPPKKKVEVQPETVKPVSPVKPDVVPTAAKINGSQGSTNDKQEN
KQEEPNDNEEDDDEQEEGQKIKPVTNQRSQGNTAARYAQRLLRDDSNSDSSSADELSD
GDSDLSDSPAPVKRPRIVPTPSSAKLVTANGNGRATGSQSQASPAPNPVRTTMPQPHP
SAVRTSSATPSSPNPAPQTRLTGLNLLSSLASQAIPDVRERVTGVLPVAPKVNSKPSQ
PKEDTDSDSNSDSDSDSDSDSNSDSDAESDGEETNGIPLSKMASKTKQPKKKKRNTGF
MGLMKDAFAGSVKSR"
gene <483229..>485949
/gene="MCM4"
/locus_tag="AWJ20_4769"
/db_xref="GeneID:30036936"
mRNA <483229..>485949
/gene="MCM4"
/locus_tag="AWJ20_4769"
/product="MCM DNA helicase complex subunit MCM4"
/transcript_id="XM_018881862.1"
/db_xref="GeneID:30036936"
CDS 483229..485949
/gene="MCM4"
/locus_tag="AWJ20_4769"
/inference="similar to AA sequence:KEGG_Orthology:K02212"
/note="Essential helicase component of heterohexameric
MCM2-7 complexes; MCM2-7 complexes bind pre-replication
complexes on DNA and melt DNA prior to replication; forms
an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in
G1; homolog of S. pombe Cdc21p; GO_component: GO:0031261 -
DNA replication preinitiation complex [Evidence IDA] [PMID
9554851]; GO_component: GO:0042555 - MCM complex [Evidence
IEA]; GO_component: GO:0042555 - MCM complex [Evidence
IDA] [PMID 12480933]; GO_component: GO:0097373 - MCM core
complex [Evidence IDA] [PMID 13679365]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 10559985];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
10704410]; GO_component: GO:0005656 - nuclear
pre-replicative complex [Evidence IDA] [PMID 16824194];
GO_component: GO:0005656 - nuclear pre-replicative complex
[Evidence IDA] [PMID 9335335]; GO_component: GO:0043596 -
nuclear replication fork [Evidence IDA] [PMID 16103218];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
10559985]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 10704410]; GO_component: GO:0031298 -
replication fork protection complex [Evidence IDA] [PMID
16531994]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0043140 - ATP-dependent 3'-5'
DNA helicase activity [Evidence IDA] [PMID 13679365];
GO_function: GO:0004003 - ATP-dependent DNA helicase
activity [Evidence IDA] [PMID 15723534]; GO_function:
GO:1990163 - ATP-dependent four-way junction helicase
activity [Evidence IDA] [PMID 13679365]; GO_function:
GO:0003677 - DNA binding [Evidence IEA,IEA]; GO_function:
GO:0003678 - DNA helicase activity [Evidence IEA];
GO_function: GO:0003678 - DNA helicase activity [Evidence
IDA] [PMID 18657510]; GO_function: GO:0003688 - DNA
replication origin binding [Evidence IDA] [PMID 11756674];
GO_function: GO:0003688 - DNA replication origin binding
[Evidence IDA] [PMID 16824194]; GO_function: GO:0004386 -
helicase activity [Evidence IEA]; GO_function: GO:0016787
- hydrolase activity [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0003697 - single-stranded DNA binding
[Evidence IMP] [PMID 17895243]; GO_function: GO:0017116 -
single-stranded DNA-dependent ATP-dependent DNA helicase
activity [Evidence IDA] [PMID 18657510]; GO_function:
GO:0043142 - single-stranded DNA-dependent ATPase activity
[Evidence IDA] [PMID 15723534]; GO_process: GO:0006260 -
DNA replication [Evidence IEA,IEA]; GO_process: GO:0006270
- DNA replication initiation [Evidence IEA]; GO_process:
GO:0006270 - DNA replication initiation [Evidence IGI]
[PMID 2044962]; GO_process: GO:0006271 - DNA strand
elongation involved in DNA replication [Evidence IMP]
[PMID 10834843]; GO_process: GO:0006268 - DNA unwinding
involved in DNA replication [Evidence IDA] [PMID
13679365]; GO_process: GO:0006268 - DNA unwinding involved
in DNA replication [Evidence IDA] [PMID 15723534];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0000727 - double-strand break repair via
break-induced replication [Evidence IMP] [PMID 20516198];
GO_process: GO:0033260 - nuclear cell cycle DNA
replication [Evidence IMP] [PMID 10834843]; GO_process:
GO:0033260 - nuclear cell cycle DNA replication [Evidence
IMP] [PMID 2044962]; GO_process: GO:0006267 -
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [Evidence IDA] [PMID 16824194];
GO_process: GO:0006267 - pre-replicative complex assembly
involved in nuclear cell cycle DNA replication [Evidence
IPI] [PMID 9335335]"
/codon_start=1
/product="MCM DNA helicase complex subunit MCM4"
/protein_id="XP_018736299.1"
/db_xref="GeneID:30036936"
/translation="MASSPPAPGDSSPLFFNSVTSSSPIRARASQDDGALSERAGRLR
IEDVSSPLGYPSSERDHTLYSASRNGRSSHFSETDNISADNHHRSYNRRGDIFSSDLT
PSRRMLVSSDGHAPSDSGLFTLSARGSDVSETGNPGDPVRLIWGTNVSIAETMNSFRS
FLQSFKSKYRMMHDDIPIGPGEGEDLVYVNMMNQMRILGSANLNLDAQNLLAYPATHK
LYYQLINYPYELVPIMDQVIKDCMVTLVTTTTNGGTVDAKELEATEMRNYKVRPFGID
NKRGMRDLNPGDIDKLVTVKGLVLRATPVIPDMKEAFFKCIVCSHTVVVEIDRGTITE
PTKCPRPVCGRTNSMQIVHNRCLFADKQVIRLQETPDITPDGQTPHSISLCVYDELVD
ICKAGDRVEVTGIFRSVPVRINPRQRTLKSLFKTYVDILHVQKVDKKRMGIDVTTNLE
AEVAEQQQADVEETRKITEDDIAKIKEVAARDDLYDLLARSLAPSIYEQDDVKKGILL
QLFGGSNKTFEKGGSPRYRGDINILLCGDPSTSKSQMLQYVHKIAPRGIYTSGKGSSA
VGLTAYVTRDIETKQLVLESGALVLSDGGICCIDEFDKMSDATRSVLHEVMEQQTISI
AKAGIITTLNARTSILASANPIGSKYDVKLSVPQNIDLPPSLLSRFDLVYLMIDKIDA
QADRHLANHLTRMYAEDVPENAATSEILPVEFLTLYISYAKNLQPQITPEAKDELVKA
YVAMRKLGEDSRTAEKRITATTRQLESMIRLSEAHAKMRLSEVVTADDVLEAVRLIRS
AIKEYAMDPITGTIDMDLVQTGTSSAQRRLRVDLKENLLSIMDTAIARTGSGGTLRYD
ELVRLVADIIPTRVENLELGNALRSLEQEGKIVMTGSNTRRTIRRVTGLV"
gene complement(<486060..>488579)
/gene="SLS1"
/locus_tag="AWJ20_4770"
/db_xref="GeneID:30036938"
mRNA complement(<486060..>488579)
/gene="SLS1"
/locus_tag="AWJ20_4770"
/product="Sls1p"
/transcript_id="XM_018881864.1"
/db_xref="GeneID:30036938"
CDS complement(486060..488579)
/gene="SLS1"
/locus_tag="AWJ20_4770"
/note="Mitochondrial membrane protein; coordinates
expression of mitochondrially-encoded genes; may
facilitate delivery of mRNA to membrane-bound translation
machinery; GO_component: GO:0016021 - integral component
of membrane [Evidence IDA] [PMID 8917313]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence
IEA,IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IDA] [PMID 8917313]; GO_component:
GO:0042645 - mitochondrial nucleoid [Evidence IDA] [PMID
12637560]; GO_component: GO:0042645 - mitochondrial
nucleoid [Evidence IDA] [PMID 15692048]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 16823961];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0009060 - aerobic respiration
[Evidence IMP] [PMID 8917313]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IMP] [PMID 12637560];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="Sls1p"
/protein_id="XP_018736300.1"
/db_xref="GeneID:30036938"
/translation="MLSFGGRAGRAICLLPKRGSVGVLSKAGNIINSCTTYGYGIRWN
SGPGSDNLGDTKNELLSSKEGMKGIAVDDISSLKKSPLLNQKDSTPAAAADTAGSSST
TIDSAAGSNTSTVDSQSETKFSGSETTENVTGNSHSLAASKTRGKKLPPPPQEYIILG
ARSSRVIPKHRRPKTNFSRDIKVDDLDEQRKQLLVFTSSVPIGTAIANIDRMKPSASL
LSPKRYDQLVIDLKRSFAVPQLREYIREKSNSTLRSSGTKAQLINTILLSLWRVSASQ
SVSESSDVIIERTFQLTRRELFIIVSQNSKLPRSWTKSGASIVILGDEQKIIVRATAD
TCNWIMASLHKTLQSIQDNDIDFSCLEPFASINDIPLDQIQRLSDTYLRYQQSEKMLV
ASSKSKDHAEHARRLILHSSGFTPKSISNYIYEDSNFTPRSLSRIIDDDALAWNERSR
IWKRWKKVRSKQPFSSFERLRNLGSQPKNIVLAQGKINKVAPQPSTLKDSYEDSVSNI
LLKNLEQEDSNNQLTFTATFGYLLHEAPSESNAKSAADPSYLAGDVQRTFLSNVPFVF
QRCSELSLYHNEIYHDPSKPPPPPKPSPDSGSTTATSSAWNKLLSNAKLLTDSVPSTE
DDSLVPVEGTRGLLDDDNMRLVQIKFIPSPFHLKDSDKLQKLPPVEMWFELKENSDEC
DLDSLRLVSADHEANSYLSLPDYETDIKFSATSANFLDHNEQEGVNQFLSRAQLGISG
NKKIYVPNELKLKMPGFEDDVTYLYQSMCYRRQIDLGFKGHILQLSSLESGGFGGRRI
EANLVLDFPDGSESVSKSEVKSFVNDALEFCESLRRRSIHL"
gene <488843..>490696
/gene="rrn5"
/locus_tag="AWJ20_4771"
/db_xref="GeneID:30036939"
mRNA <488843..>490696
/gene="rrn5"
/locus_tag="AWJ20_4771"
/product="RNA polymerase I upstream activation factor
complex subunit Rrn5"
/transcript_id="XM_018881865.1"
/db_xref="GeneID:30036939"
CDS 488843..490696
/gene="rrn5"
/locus_tag="AWJ20_4771"
/codon_start=1
/product="RNA polymerase I upstream activation factor
complex subunit Rrn5"
/protein_id="XP_018736301.1"
/db_xref="GeneID:30036939"
/translation="MPPKRKSLPDISNEGPSQKKLFLQADYRIKYGKPQLEKYVAQFN
KEVVEFLTGIDTSDSGEQVDFVSEKDNDAGTEEPLVDDVSERTEPAINGSEAVTSKSE
VVTNILEVDSDSEHSTNGVLNRVDECSSSEDSIGIDETDLEPSEDDYRCGTIWSEEEK
ERFFTALGRKSCHDMAAISRAVGTKTLIECEAYYNLLKSCYEYLKRRKPLLSKTLQRE
IFVSYEDLPYSYEMTESWDSVENKYANAVIDAIADMEVEKQNQQQETTNDQNGTTEHT
NSEPLLLKLDTDTSSQHPIDTTLINGSSHEHPNTGTLQGVGSEISVSNVASSDYKVSA
TRTATTVATNVVPRKTLFAKRSGMCAKASSQLLNIPEAVKFANMFFRQSQKNLGHMYH
HGGRGQKKSHFYLEDISQEAVDYLENIIKSQTRLILGRYIWSAEDYDPSDEEHRNKAF
YENLVHAMGYITRVNKHLQERYPRATFWKAISDFNGVEIDTYNTPPLQKNPADEYRFH
FGVEPKHQNRFPFGLKQQFQPYEHLAYSQPTIDVEDSYSPQQRRFFQQVLEAAQEVAQ
RRPEDSTSPSDIVKSSNTDDDIDRYLDLVEIDISRHEEKRLLVWFNGAEAT"
gene <492519..>494135
/gene="CRH1"
/locus_tag="AWJ20_4772"
/db_xref="GeneID:30036940"
mRNA <492519..>494135
/gene="CRH1"
/locus_tag="AWJ20_4772"
/product="Crh1p"
/transcript_id="XM_018881866.1"
/db_xref="GeneID:30036940"
CDS 492519..494135
/gene="CRH1"
/locus_tag="AWJ20_4772"
/note="Chitin transglycosylase; functions in the transfer
of chitin to beta(1-6) and beta(1-3) glucans in the cell
wall; similar and functionally redundant to Utr2;
localizes to sites of polarized growth; expression induced
by cell wall stress; GO_component: GO:0031225 - anchored
component of membrane [Evidence IEA]; GO_component:
GO:0005618 - cell wall [Evidence IEA,IEA,IEA];
GO_component: GO:0005576 - extracellular region [Evidence
IEA]; GO_component: GO:0009277 - fungal-type cell wall
[Evidence IDA] [PMID 10757808]; GO_component: GO:0009277 -
fungal-type cell wall [Evidence IDA] [PMID 15781460];
GO_component: GO:0000131 - incipient cellular bud site
[Evidence IDA] [PMID 10757808]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0016798
- hydrolase activity, acting on glycosyl bonds [Evidence
IEA,IEA]; GO_function: GO:0004553 - hydrolase activity,
hydrolyzing O-glycosyl compounds [Evidence IEA];
GO_function: GO:0016757 - transferase activity,
transferring glycosyl groups [Evidence IGI,IMP] [PMID
18694928]; GO_function: GO:0016757 - transferase activity,
transferring glycosyl groups [Evidence IDA] [PMID
23919454]; GO_process: GO:0005975 - carbohydrate metabolic
process [Evidence IEA]; GO_process: GO:0006037 - cell wall
chitin metabolic process [Evidence IGI,IMP] [PMID
18694928]; GO_process: GO:0071555 - cell wall organization
[Evidence IEA]; GO_process: GO:0031505 - fungal-type cell
wall organization [Evidence IGI,IMP] [PMID 18694928];
GO_process: GO:0008152 - metabolic process [Evidence IEA]"
/codon_start=1
/product="Crh1p"
/protein_id="XP_018736302.1"
/db_xref="GeneID:30036940"
/translation="MVGKHSFLSAVAIALISPTVNAQTFTTCNPLSSNDCPADPALGG
PLFVDFTQGSSNDFSPLTAASKISYNDLGVGFTVAQQGDNPTLASNGYIMFGRIEVVL
QAAPGQGMVSSVILQSDDLDEIDMEWIGGDNTQFQSNYFSKGQTTTFDRGEFHSVNDP
VGSFHNYTVDWTADQTTWYLDGAPVRVLESNAPEGYPQTPMKLRMGSWAGGDPSNPPG
TIEWAGGPTNYAAGPFTFYVKSVYYVDYSTGSEYVYGDESGSWQSIKAIGGSVIGSPV
SPPAVNALAAVASSADPPSSLPPVASSTPAMSSSASPPPPPPPSPTPSSSAIPSSSIE
SSPTIFSSSVATSTLSSSSSIFSSSSPPPPPSTSTPSSSSLFTSSSLPPPPPPSTTST
TSTPSSSSAPPSSSSTTSSTTSSIESSSSSSVEPSTTSSSSTPPPPPPPPTTTSAIRS
SSLTSTSSTTSSTTSSTTSTTTSSTISTTSTTSINTTTSLPPPPSTSSSTSASTTPSI
VPPSEGAASLSSIPSFVSLTIVIISVILHV"
gene complement(<494344..>495246)
/locus_tag="AWJ20_4773"
/db_xref="GeneID:30036941"
mRNA complement(<494344..>495246)
/locus_tag="AWJ20_4773"
/product="hypothetical protein"
/transcript_id="XM_018881867.1"
/db_xref="GeneID:30036941"
CDS complement(494344..495246)
/locus_tag="AWJ20_4773"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736303.1"
/db_xref="GeneID:30036941"
/translation="MNDRPPESPSGIKVGEDGARYVGGSVRSDGSVRKTVKIRPGFVP
AEDVPKYVIRARRTEGPGNASSGGPGGASRPQFRPSAAFGLITKTLQDNRTADKRDNA
RSGPDQTDDLADALSKVSIADKTSRDEKRVEGSDSDTAKKNGLKERSGASRPDDTVSK
TRSEFTKHLKSKTESSQLASEEEPIGLKSKSMRHPIRNSRSKDKKLNPTTSQKPSEKE
QKDSSKSIESSEKSNDRESKASLSPETTEKEQNRSSKSIEPTGRTIPTSKDAERSSPA
STTSEKGGSRAYVPPWKRNNDKRA"
gene complement(<496087..>497916)
/gene="ASH1"
/locus_tag="AWJ20_4774"
/db_xref="GeneID:30036942"
mRNA complement(<496087..>497916)
/gene="ASH1"
/locus_tag="AWJ20_4774"
/product="DNA-binding transcription repressor ASH1"
/transcript_id="XM_018881868.1"
/db_xref="GeneID:30036942"
CDS complement(496087..497916)
/gene="ASH1"
/locus_tag="AWJ20_4774"
/codon_start=1
/product="DNA-binding transcription repressor ASH1"
/protein_id="XP_018736304.1"
/db_xref="GeneID:30036942"
/translation="MVSSLVLPAPRSSDLLMRDRSPEIMSLRGGAINTTSPRSSGLVP
VSSVGNNSNNGSGSVSGSGTSSGLARTVPDSDLNQRDYDSIPNDNEVVNGADSSTGAG
AATIGYDTNVNSVSAASTPSSTSVFTSQSSSSTTASTPIPFFPSTGQSDQSSKSTGHE
STHNEYFSSATDAPLPGSNSTLHTDSLPTNLQPEESSSPVVSIELPKTSSSSDGSIDE
ETLSVSSITDTPSTSLGVAQKDSKSYSHVASPLTPTSLAHLSPPTVSLLYPDTAPRET
WRPQLDAWMKKNHPLKLKELSQISYPWIHDKLPSTAPINFPSRPHSHTSTSSSSSSHR
DTTSTSTSSSSSHSVPPSSRSQETSPPYPPATLSTTSIYGKAGLSKVSSSNSILPLYA
SSASHDRTSTASSMSPTTQYLSPSLSIPSSSASSTSSGSLSMSVPTVSSSSYHRPQKA
ARRASYSATSHTKPYSRPNHSTIGGPTGLSQEDDDRDDHAHIHNPRLPSPPESPQLSA
NSNSQLTLEQYTNSIKRRCISCGSDQSPCWRPSWSASAGQLCNSCGLRYKKTGARCLS
AKCGRIPAKGEWAAMKSVAVRVGGQLHYSCLWCGDEVEVMPRG"
gene <501045..>501920
/locus_tag="AWJ20_4775"
/db_xref="GeneID:30036943"
mRNA <501045..>501920
/locus_tag="AWJ20_4775"
/product="hypothetical protein"
/transcript_id="XM_018881869.1"
/db_xref="GeneID:30036943"
CDS 501045..501920
/locus_tag="AWJ20_4775"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736305.1"
/db_xref="GeneID:30036943"
/translation="MSSSSSRKHLVGVKVLQLSRPVLSRAEILKETPSTLVELPSSFN
VYVGEKFSCLVSVYSESTQPVHVNLSVFVVTPSHKETVLVHSDQPEVSEFTLEPGKST
QLTVHYEPKSSGSHSLTTAISYRPKVYESAEDLSDEGSGSPSTQQTPGKTNSSTPTGT
SGTQADQNPITNPIVKFHKRNEFLATQALEVRGKVTFIDGNQSAYTFEAEIENVSHNT
MILETVDLLPGTGWLATASPNTPDTPLHPKDVWQMAYIVTTDPDATTVTSARPDKFML
GWRREPLGERGWLAL"
gene complement(<502142..>502729)
/gene="SGN1"
/locus_tag="AWJ20_4776"
/db_xref="GeneID:30036944"
mRNA complement(<502142..>502729)
/gene="SGN1"
/locus_tag="AWJ20_4776"
/product="Sgn1p"
/transcript_id="XM_018881870.1"
/db_xref="GeneID:30036944"
CDS complement(502142..502729)
/gene="SGN1"
/locus_tag="AWJ20_4776"
/inference="similar to AA sequence:KEGG_Orthology:K14396"
/note="Cytoplasmic RNA-binding protein; contains an RNA
recognition motif (RRM); may have a role in mRNA
translation, as suggested by genetic interactions with
genes encoding proteins involved in translational
initiation; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 10764794]; GO_function: GO:0003723 - RNA
binding [Evidence IEA]; GO_function: GO:0003676 - nucleic
acid binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0008143
- poly(A) binding [Evidence IDA] [PMID 10764794];
GO_process: GO:0016071 - mRNA metabolic process [Evidence
IGI] [PMID 10764794]"
/codon_start=1
/product="Sgn1p"
/protein_id="XP_018736306.1"
/db_xref="GeneID:30036944"
/translation="MSENNQATEAVTSQEPQDVEINHDEAQEHSEQAPSGDGEQTVDA
MKTRLKELEAEAERLQAMQSELQKEGADLTEDKADVDSRSVYVGNVDFGSTPEELQQH
FQSCGTINRVTILIDKFTGQPKGYAYVEFAEPSLVSQALLLNESIFRGRPLKVTPKRT
NIPGMSARGRGRGGRGGRGYRGGFRGRGRGGYRPY"
gene <505064..>505726
/locus_tag="AWJ20_4777"
/db_xref="GeneID:30036945"
mRNA <505064..>505726
/locus_tag="AWJ20_4777"
/product="hypothetical protein"
/transcript_id="XM_018881871.1"
/db_xref="GeneID:30036945"
CDS 505064..505726
/locus_tag="AWJ20_4777"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736307.1"
/db_xref="GeneID:30036945"
/translation="MRGKYVHEIVSHKVIPARAYDYVQLVSKIYPAIAQDPKNGVHLV
GSWRTVIGDQDTYTHIWEYNGFEGFHRTQNVIHEDPKYLQYLGELRECLRSRESSIMQ
EFSFWGGTAAPRELGGIFELRSYDLKAGCLLEWETHWKKGIECRRQVMEPVGAWFSQI
GSLNRVHHLWQFADLEHRKLSRARSWEIPGWAETVSAMIFICVSFCNTNYSFRLTRPL
NS"
gene complement(<506179..>508368)
/gene="RSM22"
/locus_tag="AWJ20_4778"
/db_xref="GeneID:30036946"
mRNA complement(<506179..>508368)
/gene="RSM22"
/locus_tag="AWJ20_4778"
/product="mitochondrial 37S ribosomal protein RSM22"
/transcript_id="XM_018881872.1"
/db_xref="GeneID:30036946"
CDS complement(506179..508368)
/gene="RSM22"
/locus_tag="AWJ20_4778"
/note="Mitochondrial ribosomal protein of the small
subunit; also predicted to be an
S-adenosylmethionine-dependent methyltransferase;
GO_component: GO:0005763 - mitochondrial small ribosomal
subunit [Evidence IPI] [PMID 11278769]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA]; GO_function:
GO:0008757 - S-adenosylmethionine-dependent
methyltransferase activity [Evidence ISM] [PMID 19351663];
GO_function: GO:0008168 - methyltransferase activity
[Evidence IEA,IEA]; GO_function: GO:0003735 - structural
constituent of ribosome [Evidence IPI] [PMID 11278769];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_process: GO:0032259 - methylation [Evidence IEA];
GO_process: GO:0032543 - mitochondrial translation
[Evidence IC] [PMID 11278769]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="mitochondrial 37S ribosomal protein RSM22"
/protein_id="XP_018736308.1"
/db_xref="GeneID:30036946"
/translation="MFSIQRRLVQRARLSSPQLVRYRSSAPSARGTGRLSGVKAIPYA
EIVNEHSDLQDNVEVLNLEELINASNEPNFAEENRSARTERLDIDTRQGKTAGNKLLV
LPEEITSVISNATRGYTPNRLRSNASKYYLSLAESGAHRPATETIDVDTHLTGVFLQN
YASLYNVLKEARQRLGEKWRPNRILDVGFGPATGMIALNEVFSDVDAEDWQPERKLAI
VIGHRDMKKRAVSVLATQKHEESEEQGQLFEENEEALTEEEQQELEEILAVETKGLEE
TQQEELDVNTEETDSVNEIEFEEIPKTKQKAHIKTVVRQQLPAAKSSSKYDLIIATHQ
LYRSGYHYPASVDDHTSHLLSLLSPGGILVLVERGDPTGFESIARARQIMFRPEDHQS
PEKYPPRLWKNDEQFPLSIVAPCSHHGKCPLQVELGIRNKGKNPAYFGWCKFAQMVQR
PKFALELKKGQLLSQKWSLVNTASGKVSGEGGKSLAGSGRAFGRSFETATHSYLIVER
KAKEQKGTNEQSQELELKDDSISDPASWPRILQNPLKRDKHVVMEVCAPSGQVEQWTV
TQKFGKQAYHDARKATGGDLWALGAKVKQVRGGNLSKLRKASAKIQSESRRATKENLQ
AEIKIDEKVAAEIQEENVDSESESKAPDAIDIAWLRNDSFNSQKMKRKQAYTRGSEPG
AYEDIDGGFSSEYFDDLAQVFENKKAKDYAKKLEKSSHVEKKRVMKW"
gene <508852..>509604
/gene="FSH3"
/locus_tag="AWJ20_4779"
/db_xref="GeneID:30036947"
mRNA <508852..>509604
/gene="FSH3"
/locus_tag="AWJ20_4779"
/product="Fsh3p"
/transcript_id="XM_018881873.1"
/db_xref="GeneID:30036947"
CDS 508852..509604
/gene="FSH3"
/locus_tag="AWJ20_4779"
/note="Putative serine hydrolase; likely target of
Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is
similar to S. cerevisiae Fsh1p and Fsh2p and the human
candidate tumor suppressor OVCA2; GO_component: GO:0005575
- cellular_component [Evidence ND]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0008150 - biological_process [Evidence
ND]; GO_process: GO:0008152 - metabolic process [Evidence
IEA]"
/codon_start=1
/product="Fsh3p"
/protein_id="XP_018736309.1"
/db_xref="GeneID:30036947"
/translation="MSQKILCLHGFTQNGPLFARKVSAVRKTLQKAGFETVFLTAPVK
LEIADLPFEPTNVASLGGSDDSMRSWWPNSDSNPNHYKLDQAFEAIKQSIIEDGPYVG
VLGFSQGAALAGVLCQHIHKLHETQPVLKFGIFYSGFRIMRPEHQHFYNELISVPTLH
ILGSLDTVVSEERSMALYNSCSDNTRTLLHHPGGHFVPNSKDFVTRVVEMVVSSTSSS
PPATEPDVVNEKISETSQPSDDWSAFDTIGRA"
gene complement(<511059..>514622)
/gene="KEL1"
/locus_tag="AWJ20_4780"
/db_xref="GeneID:30036949"
mRNA complement(<511059..>514622)
/gene="KEL1"
/locus_tag="AWJ20_4780"
/product="Kel1p"
/transcript_id="XM_018881875.1"
/db_xref="GeneID:30036949"
CDS complement(511059..514622)
/gene="KEL1"
/locus_tag="AWJ20_4780"
/note="Protein required for proper cell fusion and cell
morphology; functions in a complex with Kel2p to
negatively regulate mitotic exit, interacts with Tem1p and
Lte1p; localizes to regions of polarized growth; potential
Cdc28p substrate; KEL1 has a paralog, KEL2, that arose
from the whole genome duplication; GO_component:
GO:0005938 - cell cortex [Evidence IDA] [PMID 23673619];
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 9786949]; GO_component: GO:0005934 - cellular
bud tip [Evidence IDA] [PMID 9786949]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 19053807]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 9786949];
GO_component: GO:0005628 - prospore membrane [Evidence
IDA] [PMID 24390141]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0000755 -
cytogamy [Evidence IGI,IMP,IPI] [PMID 9786949];
GO_process: GO:0001100 - negative regulation of exit from
mitosis [Evidence IMP,IPI] [PMID 12234925]; GO_process:
GO:0008360 - regulation of cell shape [Evidence IMP] [PMID
9786949]"
/codon_start=1
/product="Kel1p"
/protein_id="XP_018736310.1"
/db_xref="GeneID:30036949"
/translation="MSYDLTTLRSENAQWTLVQPEGGISPPPRTNHTVVSYQDKLYLF
GGTDGKLWYSDTWCYDPSINTWTSLDCSGFIPAPCEGHAATIVGDIMYVFGGRSSQGK
DLGTLSALKLTTKKWFTFQNLGPGPSPRSGHSMTAFAGNKILVMGGECPELHENDNDV
NYDNYEVNSKLSMVFVLDTLRINYPVDSDVPQNSAVSDLSGKRSVNDSRTPSAASGNY
RTATGDSSTPPSTFSTASTSSVPPPHGVPVNSGTGIAGAGAGAVAAAAAMGAGVTGFP
HPESSQHEANTESSPRGVARDEYTREAVSPYQEYPSGAKSNGSGSGNRNANGNGNGGY
GSYNAGNNGVASQDVQRSPYDDGSDGVSNTTFESADEEKFKTSPSHDQVANIPGAWQQ
ASSPSSTSSRDSESYREPIKLPKLRTSPRVDTSAKGLAGIAGLAGIAAGTAGAGATAG
LGSGSGADADFSQSTRDYTEENATTPTVSNMSQDRSVDSFTDQHPVDQSTPRGSQVNL
AEPANANNVNQVIEQLKASNSWYETELAAAKEAGYVSKSNPPVDVLKLRRVSQRLTQD
NQESLSEREILITALSDLKGELRTVQGDIQRQAAAASDRISAVEKERDAALAELKSLK
ESSQPVGVPEQHQREIDDHKATIASLQNELETTRASRGLLLGNADSLSALDDLKMSNA
QLEQQLRNQADRLVIAEHEAAQFKSKHEDLQAQHKELTSSAAEKVAALAAAGAAITAS
QSKFKELSKQLDEHKSARSDLEAKNLDLERQLSDHKLQLEASQRELNDHRNLLQAAEE
QNEHSSKALGDGIDKIVSMWSGSKYFAVAGVGAGLGAAAGAAAANGTASRGLDEDDEE
DPRISHLQSQLEMANKLHVSHKEAAEKATDDLTVALTQVSGLKKELAESEAARKQAED
KLALLEEELKSTSGDLQSHRELLDNHKKSLSELTEKHDALTTQLSSQSEAHTTQAKEL
DAKLEKALKEHQETAEQRYQELEQQYKETLQYVRNSDKALNKTRDELNKHKDLNSKLQ
EEINELKLRSQQDDDEADTTTGERSINSIGSNYNNKHFDLQLRDLKAQIIILQEERDE
LRASSLELKKKAINNAHDIEEAKTEKSRLAQENAKLQQRAREAEEKLSVLLHDKRSSR
DPIIDPESGQTLDSFSDNLEKIRTERERLSGLLRESPGADE"
gene complement(<514937..>515938)
/locus_tag="AWJ20_4781"
/db_xref="GeneID:30036950"
mRNA complement(<514937..>515938)
/locus_tag="AWJ20_4781"
/product="hypothetical protein"
/transcript_id="XM_018881876.1"
/db_xref="GeneID:30036950"
CDS complement(514937..515938)
/locus_tag="AWJ20_4781"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736311.1"
/db_xref="GeneID:30036950"
/translation="MAIFSKNKKNKKDGARNSVVPKAFEGAKDALVGGAGGEEGSSPT
QRQVSGASTVSSTNSMISGNGSQSPHNGHGAGNVPNIANTISNLYSGPGSSGPYPGRS
PGSGSGGPNGASPQNRNYPQSGSRSNSAMSGHGPNGSSGGVRSPPGSRNATSPMGQQY
PGGQQQWGGQQQWQQSPQQQQLHHQQSVPSNLRSQSSPHPSSQPQYPWSRHTISNSSP
FPRHGHAANAVAARDGEVFVMGGLKGSNVFGDLWVIESGKSVGGQDASGGRALPGPVC
ARFASPKGTGCLRRPGAARTRCAPLRGAVKGSLGLAKRAQTGPGSARPPEAAKAPEI"
gene complement(<521591..>522196)
/gene="SFP1"
/locus_tag="AWJ20_4782"
/db_xref="GeneID:30036951"
mRNA complement(<521591..>522196)
/gene="SFP1"
/locus_tag="AWJ20_4782"
/product="zinc-coordinating transcription factor SFP1"
/transcript_id="XM_018881877.1"
/db_xref="GeneID:30036951"
CDS complement(521591..522196)
/gene="SFP1"
/locus_tag="AWJ20_4782"
/inference="similar to AA sequence:KEGG_Orthology:K19487"
/codon_start=1
/product="zinc-coordinating transcription factor SFP1"
/protein_id="XP_018736312.1"
/db_xref="GeneID:30036951"
/translation="MPPVPVSMSPINSTSYLPNLEADFCKDYSCCGQILPTLHDLLRH
YEEAHISPSPPVEPPVMRRMQSLEAVSTNEVFLSPVSSATATPMMMDEDDEMCIDDPA
RHLYVMERGEHKPFKCPVIGCEKTYKNQNGLKYHRMHGHQNQTLHLNEDGTYSIIDPT
SNTPYPEGMGMEKDKPYRCEVCGKRYKNLNGLKYHRGHSTH"
gene complement(<529692..>532442)
/gene="ADP1"
/locus_tag="AWJ20_4783"
/db_xref="GeneID:30036952"
mRNA complement(<529692..>532442)
/gene="ADP1"
/locus_tag="AWJ20_4783"
/product="putative ATP-dependent permease ADP1"
/transcript_id="XM_018881878.1"
/db_xref="GeneID:30036952"
CDS complement(529692..532442)
/gene="ADP1"
/locus_tag="AWJ20_4783"
/note="Putative ATP-dependent permease of the ABC
transporter family; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11914276]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IDA] [PMID
11914276]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 19001347]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0016887 - ATPase
activity [Evidence IEA]; GO_function: GO:0042626 - ATPase
activity, coupled to transmembrane movement of substances
[Evidence ISS] [PMID 9020838]; GO_function: GO:0017111 -
nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_process: GO:0006200 - ATP catabolic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055085 - transmembrane
transport [Evidence ISS] [PMID 9020838]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="putative ATP-dependent permease ADP1"
/protein_id="XP_018736313.1"
/db_xref="GeneID:30036952"
/translation="MCNVTNRKILDILAGKIPQVTFSCNSTSAECDFQFWVDQVESFY
CGLSNCTFETEVLSNTNITKYQCPEISCKCVPERMLCGEAGSIDISDFLTETISGPGF
FECDRSTKSCKFSEPSMNDLIKSVFGDPYITLECDSSECLHYTELPGYLPPEKSVRRG
FVLAAFISALVFILGGAVIFQYFMNRSQNNKLGYLQIPSDSDAAKLMANHQATSLQFE
DISYSDKKRPVKILDGVFGQVKEGSVLAIMGGSGAGKTTLLDILASKTKRGVTEGHIY
VNGREVISKNRYKKVIGFVDQEDCLIPTLTVYETIVTSALLRLPKSMSDDAKKLRALE
TMNELGILGLKDQLVGNETNRGISGGEKRRVAIACELVTSPSILFLDEPTSGLDAYNA
FNVISSLVHLARNYNRTVVFTIHQPRSNIVAQFDELMLLAKGSVVYSGPQSEASNYFA
EIGYPCPSGYNMGDFLIDLAMEADNSGGSSSNGDSAGDDQANEHLSEGGQSNSTESDD
IHNPIPRANTIEDSTREWRHYAVHRNELSGEEAHENHLRRRISTTPAIRAPPSLSTLV
ELYKNSPLAESIRQKIQAQKQAVIDADESNLTSGYETPNQNGDGPETGDDATSPATRG
GASSIPKELKGHDKVGVFGQFRILSGRTFKNLYRNPMLLLTHYVMAVLLAVFCGVLYF
NVTNDISGFQNRLGLFFFLLALFGFSTLTTLQLFAEERIIFIRERANGYYHPIAYYLA
KVMFDIIPLRVFPPILLGLIVYPLVGLSTDGYAFLRFLLILVLFNLTAASTCLLIGIM
IQNTGVASLVGCLVMLFSLLFAGLFLNQDSMPAGAVWFKYISIFHYAYEALTVNEVRY
LTLTEKKFGLSIEVPGATILSTFGFDSGALWDDVAGLAIVFGLFLIAGYVAMHFFLVE
RR"
gene complement(<536380..>537141)
/locus_tag="AWJ20_4784"
/db_xref="GeneID:30036953"
mRNA complement(<536380..>537141)
/locus_tag="AWJ20_4784"
/product="hypothetical protein"
/transcript_id="XM_018881879.1"
/db_xref="GeneID:30036953"
CDS complement(536380..537141)
/locus_tag="AWJ20_4784"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736314.1"
/db_xref="GeneID:30036953"
/translation="MSVNMVFDAETLADGCLDALTSEAVYYEGTSIKPTEIRPAKPYP
FKEGVTLTVRQAFESDRKIKGARARSRYYLFNGEPTRAEDELRFGLDDYFEQAGKGPN
SSSNRGYRDDRRSRYRDRDGRRGRDRDQDDDGDLFPLKAGEFRESIRGSDLRGRDSGS
DRDRNYDRRPAKRGKSYRYLDRNRSRSPGRDSADEPTPSNDSRSRNLSDRLDTPWVKG
DLLEKIDNGRDELADRLDLPRTKDRRNRRKAADLF"
gene complement(<537264..>537707)
/locus_tag="AWJ20_4785"
/db_xref="GeneID:30036954"
mRNA complement(<537264..>537707)
/locus_tag="AWJ20_4785"
/product="hypothetical protein"
/transcript_id="XM_018881880.1"
/db_xref="GeneID:30036954"
CDS complement(537264..537707)
/locus_tag="AWJ20_4785"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736315.1"
/db_xref="GeneID:30036954"
/translation="MSGQDEGLEIDIDDDFSVRSVKTGAVEGAGSVASEGASVAASAG
TATGSDVAVSVEAGAEAVPAVTGAGPVADDSMDVEVTDSRKIEAPGSEGSQPGAEENA
LVPRPVAVHLIGVNDLSTDEVEKYVGYYYPRIKYRVQWVDDSSCK"
gene complement(<538006..>539226)
/locus_tag="AWJ20_4786"
/db_xref="GeneID:30036955"
mRNA complement(<538006..>539226)
/locus_tag="AWJ20_4786"
/product="hypothetical protein"
/transcript_id="XM_018881881.1"
/db_xref="GeneID:30036955"
CDS complement(538006..539226)
/locus_tag="AWJ20_4786"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736316.1"
/db_xref="GeneID:30036955"
/translation="MTVDLNLEGYNVVRQLWLMFYDKDQHNFICHNNSGGPYCKEGRY
TLWPVAVTVQAIVDAMRIFPKEIGPMMRPAFEAFDAYYSPKHHAYCASFNYDGNNDVY
YDDNAQVASCFLSAYEVTHDKYYLDKAVENVHFLMTGAEKGIYGGVRWHMGKQGSNTC
TTAEVGIACMRLARVVQNNQVYIDFAKMCADWIFERVQADDKLIADGLEPDGDHFRLN
DAKWTYNQGTPITLCSLLYNAIRDEKYYKYAEGLALAATDHNTAIFDRDTQNMDARYY
RDTTYFYQLLAEGLADFLLFFSDKAPQNVLDQIRGELLHTIDYVYKYIRDDQDGLYFQ
LFSIYEIDQKRADLFRELTGETGKPYKPHEGEREKTDAPVDQRKLVKGLMGCGAAARV
LFQTARVHPTIDPK"
gene <548268..>551057
/gene="SWA2"
/locus_tag="AWJ20_4787"
/db_xref="GeneID:30036956"
mRNA <548268..>551057
/gene="SWA2"
/locus_tag="AWJ20_4787"
/product="Swa2p"
/transcript_id="XM_018881882.1"
/db_xref="GeneID:30036956"
CDS 548268..551057
/gene="SWA2"
/locus_tag="AWJ20_4787"
/note="Auxilin-like protein involved in vesicular
transport; clathrin-binding protein required for uncoating
of clathrin-coated vesicles; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005789 - endoplasmic reticulum membrane [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IDA] [PMID 11553703]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0030276 - clathrin binding [Evidence IDA] [PMID
16687570]; GO_function: GO:0043130 - ubiquitin binding
[Evidence IDA] [PMID 18948116]; GO_process: GO:0072318 -
clathrin coat disassembly [Evidence IMP] [PMID 11146663];
GO_process: GO:0048309 - endoplasmic reticulum inheritance
[Evidence IMP] [PMID 11553703]; GO_process: GO:0032781 -
positive regulation of ATPase activity [Evidence IDA]
[PMID 16687570]"
/codon_start=1
/product="Swa2p"
/protein_id="XP_018736317.1"
/db_xref="GeneID:30036956"
/translation="MDSFAELKWSSSGTAPGTGTSSKASSKPGTPAPGQAKGLDSFAS
LAPMGTGSNKPKKLESLSLQERQNMLQKGNGNAFGTSGGSSSTGGSTWSGLDLLSGGI
ASSERLGKSTVPASGLSNGASGSGSNSLLDGFDDIPGANEISTGSNTEGNVDPETKED
NIDDLFSVFNKPKVDTAKIEKEKDYETIDRAQDRLSSGSGSRSRATPDRQIQLGDDQD
DYGDTEYNGDYDEDDYSAEEGVFGNRGHRSALNSSSNRHNSSNGGSRHSSMAGSRAES
RAESRTGSRAGSGRGGSSTPSSISSRGPPAADPRDHAIAELMDMGFSDEQAIYALAQT
DTGLDVRQAVDVLMMQAHQKATGQPVTAGSRSNSRRKQKDVPVDDISKLASDLSTQFI
SKAGSLWSMGKKNLAKAIDQYNSQPSSDGTPAWMRDAQRYVSEDESGTPPIHLDSDSD
ISIKRGRTGTNRPSRRFNDSDFTDDDTPPPSSSQLATDEAMMLESEGPRSRRNKNPAT
GHRQQPPVPPPSRRYVDDAQSNSIPVPRAVPDSARTPPPPSNTPPIAQRQQPALSRAQ
LLRERQKERKDDDVMYVSSSRRRGPTSSNQSSRAPTPSLPAQSPAAPSSRIASPKPSI
PPREAVSISPTALTMANTSREQGTEAFKRGDFTQATEHYSQALQSIPPKHLLLTLILT
NRSTCYLKCGDAKSALADAEQSLAIIGPGLGANEEVEPGKSLKDIWSKAVARKAEAYE
HLEKFQDALDSWTVLISNGYSSKQSMDGKRRCQDALSPKPKPRPVTSSTPSRVSTPAN
DGPQSAAGKAALARVKNAHATAEKNDSERFILHDSVSSRIESWRHGKEDNLRALLGSL
DNILWPESGWKKVSLAELVIPKKVKLVYMKAVARTHPDKVSSTATTEQKMIAEGVFIT
LNKAWDQFKVANNLS"
gene complement(<551288..>553303)
/gene="TFB1"
/locus_tag="AWJ20_4788"
/db_xref="GeneID:30036957"
mRNA complement(<551288..>553303)
/gene="TFB1"
/locus_tag="AWJ20_4788"
/product="TFIIH/NER complex subunit TFB1"
/transcript_id="XM_018881883.1"
/db_xref="GeneID:30036957"
CDS complement(551288..553303)
/gene="TFB1"
/locus_tag="AWJ20_4788"
/inference="similar to AA sequence:KEGG_Orthology:K03141"
/note="Subunit of TFIIH and nucleotide excision repair
factor 3 complexes; required for nucleotide excision
repair, target for transcriptional activators; relocalizes
to the cytosol in response to hypoxia; GO_component:
GO:0000439 - core TFIIH complex [Evidence IEA];
GO_component: GO:0000439 - core TFIIH complex [Evidence
IDA] [PMID 14500720]; GO_component: GO:0000439 - core
TFIIH complex [Evidence IDA] [PMID 19818408];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
22932476]; GO_component: GO:0005675 - holo TFIIH complex
[Evidence IDA] [PMID 19818408]; GO_component: GO:0000112 -
nucleotide-excision repair factor 3 complex [Evidence IDA]
[PMID 8855246]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22932476]; GO_function: GO:0000990 -
core RNA polymerase binding transcription factor activity
[Evidence IC] [PMID 19818408]; GO_function: GO:0032266 -
phosphatidylinositol-3-phosphate binding [Evidence IDA]
[PMID 15909982]; GO_function: GO:0010314 -
phosphatidylinositol-5-phosphate binding [Evidence IDA]
[PMID 15909982]; GO_process: GO:0006281 - DNA repair
[Evidence IEA]; GO_process: GO:0006974 - cellular response
to DNA damage stimulus [Evidence IEA]; GO_process:
GO:0006289 - nucleotide-excision repair [Evidence IEA];
GO_process: GO:0006289 - nucleotide-excision repair
[Evidence IMP] [PMID 7891722]; GO_process: GO:0070816 -
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 19450536]; GO_process: GO:0070816 -
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 19679665]; GO_process: GO:0006355 -
regulation of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0006360 - transcription from RNA polymerase
I promoter [Evidence IMP] [PMID 12015980]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IDA] [PMID 19818408]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA,IEA]"
/codon_start=1
/product="TFIIH/NER complex subunit TFB1"
/protein_id="XP_018736318.1"
/db_xref="GeneID:30036957"
/translation="MSAGTTVSTTYPANFKKEPGTIEISDGDRDGNRLFVWKPNNTGS
AKPPINIKLETVTQLQATPATSEKLHLKIICTVGDVSSATTPASGQTSQAPATPAAPG
SETQSYVFAFKERSVMENTKTTIQKLVQAIRAAVTEKSANGTGQNGTSNGGANGTANN
NNNNMNRPENKQAVVELDSKKLLLNLNLQQGLLRENKELMRTFQETVINGGLANDKFW
NTRIHLLRAYALTSSQKRGAYNVLGTIRPTTGSDNQINVSLTREKIHDIFDQYPIVRQ
AYSDSVPGKMTEGSFWERFFLSRLFRRLRGEKVPQTHLVDAILDTKYLTLYEEEQEGY
NNRILGIVSTTSTSSGSSITNNNSNNNINANNKRTREDDPISADNNDDNRPAGEVTDD
MYHKIVPQYLDIGGNEENDPQKLGNLPDITMQPGKSDASSISLIRSMNSLSQRMLYGN
ATSRIISGRQEPASTEVSSLGDQLKFDDLESDSVADSNIELHLVNEPGISINGKSNSH
EADDTTVIEQIRDRYSKSLQTGSSINLNDVGNNTEDLKAAVGQVTSIIELRSQESDQA
SNIWTSNEQDKRLLDQVQLCHATSVEFLRHFWLHFLSGDPNQASSISKLVTNLAKSLE
RIDAVSKAAPTPDAQNHARASLGPLKTSILTALDRYEKALEASSAEK"
gene <553633..>555303
/gene="SSZ1"
/locus_tag="AWJ20_4789"
/db_xref="GeneID:30036958"
mRNA <553633..>555303
/gene="SSZ1"
/locus_tag="AWJ20_4789"
/product="Ssz1p"
/transcript_id="XM_018881884.1"
/db_xref="GeneID:30036958"
CDS 553633..555303
/gene="SSZ1"
/locus_tag="AWJ20_4789"
/note="Hsp70 protein that interacts with Zuo1p (a DnaJ
homolog); interacts with Zuo1p to form a
ribosome-associated complex that binds the ribosome via
the Zuo1p subunit; also involved in pleiotropic drug
resistance via sequential activation of PDR1 and PDR5;
binds ATP; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 10792726]; GO_component: GO:0005844 - polysome
[Evidence IDA] [PMID 16413483]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0051082
- unfolded protein binding [Evidence IGI,IPI] [PMID
11054575]; GO_function: GO:0051082 - unfolded protein
binding [Evidence ISS] [PMID 9488429]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence IGI,IPI]
[PMID 11054575]; GO_process: GO:0002181 - cytoplasmic
translation [Evidence IGI,IMP] [PMID 11929994];
GO_process: GO:0006364 - rRNA processing [Evidence IMP]
[PMID 20368619]; GO_process: GO:0006450 - regulation of
translational fidelity [Evidence IMP] [PMID 15456889];
GO_process: GO:0006452 - translational frameshifting
[Evidence IMP] [PMID 16607023]"
/codon_start=1
/product="Ssz1p"
/protein_id="XP_018736319.1"
/db_xref="GeneID:30036958"
/translation="MSGPTVIGLAFGNTTSSIAIEKDGKVELIANQDGDRAIASVISY
VGSDEYHGAQAKAQLIRNAKNTVAYFRDFLGVSFDNVDPTNSHASAHPVELNGQASFS
LKTEDDDEETGEVKPAKLLSVHEIAVRHLDRLRESAADYIGKAIDGAVIAVPTDYSEE
RRQAIVAAAKDANLKILQVINEPTAALLAHVSANGDSTDKKYAVIDIGGTRSDGAIIA
SRGGIFTILTTLHNYELGGKNLDEALVDFFAKDFEKQHKVDPKTEARAVAKLLAESES
TKKTLSNTSSANFAVESVAQGYDYSLSINRLRFEVAARSVFTKITQFVEELIKKANLD
VLDIDEVLLVGGTSAVPKVATSIASLFDESVTKISAPAFDTKAIQPDELISRGAAIQA
SLVAGYDDEEINESLQAVVTVAPHTTKSIGVKVGDNEYVEVIASETALPIRKTVTLST
PADATAVLVGVYEGASEIVVTKVEKEEKKDDAEDDESDWSEDEEDEEIRTKVLKPAAK
IAELGLQNIPAGSKVEVVLNITRELKLQIAARAGSVAVRGETAVAHVA"
gene complement(<555554..>558037)
/gene="PXA1"
/locus_tag="AWJ20_4790"
/db_xref="GeneID:30036960"
mRNA complement(<555554..>558037)
/gene="PXA1"
/locus_tag="AWJ20_4790"
/product="ATP-binding cassette long-chain fatty acid
transporter PXA1"
/transcript_id="XM_018881886.1"
/db_xref="GeneID:30036960"
CDS complement(555554..558037)
/gene="PXA1"
/locus_tag="AWJ20_4790"
/inference="similar to AA sequence:KEGG_Orthology:K15628"
/note="Subunit of a heterodimeric peroxisomal ABC
transport complex; required for import of long-chain fatty
acids into peroxisomes; similarity to human
adrenoleukodystrophy transporter ABCD1 and ABCD2 and
ALD-related proteins; mutations in ABCD1 cause X-linked
adrenoleukodystrophy (X-ALD), a peroxisomal disorder;
complex also includes Pxa2p; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0005779 -
integral component of peroxisomal membrane [Evidence IDA]
[PMID 8670886]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_component: GO:0005778 - peroxisomal
membrane [Evidence IEA]; GO_component: GO:0005777 -
peroxisome [Evidence IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA,IEA]; GO_function: GO:0016887 -
ATPase activity [Evidence IEA]; GO_function: GO:0042626 -
ATPase activity, coupled to transmembrane movement of
substances [Evidence IEA]; GO_function: GO:0042626 -
ATPase activity, coupled to transmembrane movement of
substances [Evidence IMP,ISS] [PMID 8670886]; GO_function:
GO:0017111 - nucleoside-triphosphatase activity [Evidence
IEA]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA,IEA]; GO_process: GO:0006200 - ATP catabolic
process [Evidence IEA]; GO_process: GO:0015908 - fatty
acid transport [Evidence IMP] [PMID 8670886]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA,IEA]"
/codon_start=1
/product="ATP-binding cassette long-chain fatty acid
transporter PXA1"
/protein_id="XP_018736320.1"
/db_xref="GeneID:30036960"
/translation="MAASSTLRSDRTKRMLMMYAQLALSLAQNPRLALQLIRSQYRYS
SGRIKAVILLLALLTAGSVTFGITELQKRRKRDAKERRELHRQSSSVLLSDGWSREIF
VPTANKPLTSKSKSSTVESGEVSGDATARVVIKPTKKVTFEAHRRLFLQSGSGGKSGN
GSVAEKKAGVNRQFLKEFSAIWSIIVPRLQSKTSALLFIHALFLASRTYLSLLVAKLD
GQIVRDLIAANGKDFLRGLGYWFLLAIPASYTNAMIRFLQAKISIAFRTKLIRYIHDI
YLSKELGYYKVTNIDGGIEGADQYITADVTRFCDSAASLYSNLGKPFADFIIFSYQLQ
RNLGPLALIGIFSNYALTAWLLKSVAPPFGRLAAAEARLEGDYRNAHTKLITNAEEIA
FYDGTGFERAILDKTYKRVIVHISKILKIKVSYNMFEDFILKYSWSALGYMFASLPVF
LPTWSGNDGISELATTTPSISLAKRDFRERDRMKQFITNKRLMLSLADAGGRMMYSIK
DLAELAGYTSRVFQLLSTLHRVHANSYGHELAPNEVAEPFTLADVHGTVQQGFEGVRF
EHTPIVIPGLARTGQPGEVLIKDLNIRINPGDHILISGANGVGKSSIARVLAGLWPVY
RGLVSKPLPKDITFLPQRPYLSHGTLRDQIIYPDSHADMLEAGGTDAKLMEILKLVRL
EYIPGREGGWETRKQWKDVFSGGEKQRVMFARILYKNPKFAVIDEGTSAVSADVEGLL
YEICKKQSITLVTISHRPSLLKYHTAQLRVGLGANGDEWSLERSGTAEARLTVDKEIE
DLKAKLSQVDAWKARRQQIVDELSGKTAA"
gene complement(<558555..>558671)
/locus_tag="AWJ20_4791"
/db_xref="GeneID:30036961"
rRNA complement(558555..558671)
/locus_tag="AWJ20_4791"
/product="8S ribosomal RNA"
/transcript_id="XR_001989277.1"
/db_xref="GeneID:30036961"
gene complement(<558873..>561473)
/gene="KCH1"
/locus_tag="AWJ20_4792"
/db_xref="GeneID:30036962"
mRNA complement(<558873..>561473)
/gene="KCH1"
/locus_tag="AWJ20_4792"
/product="Kch1p"
/transcript_id="XM_018881887.1"
/db_xref="GeneID:30036962"
CDS complement(558873..561473)
/gene="KCH1"
/locus_tag="AWJ20_4792"
/note="Potassium transporter that mediates K+ influx;
activates high-affinity Ca2+ influx system (HACS) during
mating pheromone response; expression up-regulated in
response to alpha factor; localized to sites of polarized
growth; member of a fungal-specific gene family; potential
Cdc28p substrate; KCH1 has a paralog, PRM6, that arose
from the whole genome duplication; GO_component:
GO:0000324 - fungal-type vacuole [Evidence IDA] [PMID
14562095]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0005887 - integral component
of plasma membrane [Evidence IDA] [PMID 23204190];
GO_component: GO:0005937 - mating projection [Evidence
IDA] [PMID 23204190]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005774 - vacuolar
membrane [Evidence IEA]; GO_component: GO:0005773 -
vacuole [Evidence IEA]; GO_function: GO:0015079 -
potassium ion transmembrane transporter activity [Evidence
IDA] [PMID 23204190]; GO_process: GO:0071805 - potassium
ion transmembrane transport [Evidence IDA] [PMID
23204190]"
/codon_start=1
/product="Kch1p"
/protein_id="XP_018736321.1"
/db_xref="GeneID:30036962"
/translation="MYVGILLAIAAFASDIYTGIVLLAFDRWSSTIEPYVPFRISRWL
FGGCIILSVLLFIYDFIVAYTTIRTKNISLNYTDPIARNVYSIRGYRYFCLFAKLTKS
RSLTEYVALFVYFSFKGWIRLIFADSPRQVLNALTLYSVLQIQGNFVSTIKKLAQNST
VEAVVIGFMAFSLLVWVVNIIQFGLALICAVPIYIHIGDQASGLEEYCCVKVNKRIAR
LVEKYHRKGMKELAEANKKLEKQPSLPRVDTSTLDSHSRYNEKASYSFDQLSSLQPSS
SLKQDTFGPGPRTRPGEQPYRNRAGVYRNQSAPRSQTQPQLQPRANKNDTLYREYPVN
TPDLGASTTSVDSISGGITGGGGYGYDSTENDNAPLLDQNARTKTPAPVLPRNNTSQT
NIYNRRPSVPPKGANLSTPRENFVPNQSNRNANPYQTQQPNTNSNPFPTNQFNPNVNQ
YQNNGQKPYRNMATNTRVQQPYQPSNVAPTGMSNYSQAPSFGTQPNFPNTVQTSDQSQ
TGLQDYDGRPPRPDPYRLVPFPDPNAERQRPTPYPVDGRSLTSTPFNTTNPLSSYPAN
ISQGPVVPQGMELRSVPQQPVVVVDQPRPLVKQKSLPIQTQIPPKSVSRQKTMPAAIP
VNKEMPMSRKSTLLPDEIEHREPKLPFSSVPDTNLPSSRGSKLEPSDLEFREPKLPFT
STVENDPSSDESDADTFDFGKPELSAHLRRQLSAETKPSPEELKSREPKLPFESTSEE
NHSTTDHKTAADLRKSKLSPTELQFREPKLPNLQDRNPFRQSTSSLSDAEEDLIVYSN
DEISSPEQPPPFGHDNHPRGPSPVSFVFQPNAPPKAAARLRQPTIPKITFEDTSSPAS
QQSGRPSPFY"
gene <565732..>567987
/gene="PMT4"
/locus_tag="AWJ20_4793"
/db_xref="GeneID:30036963"
mRNA <565732..>567987
/gene="PMT4"
/locus_tag="AWJ20_4793"
/product="Pmt4p"
/transcript_id="XM_018881888.1"
/db_xref="GeneID:30036963"
CDS 565732..567987
/gene="PMT4"
/locus_tag="AWJ20_4793"
/inference="similar to AA sequence:KEGG_Orthology:K00728"
/note="Protein O-mannosyltransferase; transfers mannose
residues from dolichyl phosphate-D-mannose to protein
serine/threonine residues; appears to form homodimers in
vivo and does not complex with other Pmt proteins; target
for new antifungals; GO_component: GO:0097586 -
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt4p homodimer complex [Evidence IPI] [PMID 12551906];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0004169 -
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [Evidence IEA]; GO_function: GO:0004169 -
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [Evidence IDA,IMP] [PMID 21956107]; GO_function:
GO:0004169 - dolichyl-phosphate-mannose-protein
mannosyltransferase activity [Evidence IMP,ISS] [PMID
8585318]; GO_function: GO:0000030 - mannosyltransferase
activity [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_function:
GO:0016757 - transferase activity, transferring glycosyl
groups [Evidence IEA]; GO_process: GO:0006493 - protein
O-linked glycosylation [Evidence IEA]; GO_process:
GO:0006493 - protein O-linked glycosylation [Evidence
IMP,ISS] [PMID 8585318]; GO_process: GO:0035269 - protein
O-linked mannosylation [Evidence IMP] [PMID 18182384];
GO_process: GO:0035269 - protein O-linked mannosylation
[Evidence IMP] [PMID 21231968]; GO_process: GO:1900101 -
regulation of endoplasmic reticulum unfolded protein
response [Evidence IGI] [PMID 21231968]"
/codon_start=1
/product="Pmt4p"
/protein_id="XP_018736322.1"
/db_xref="GeneID:30036963"
/translation="MPSELRNRKGASATLRSPEPVEKKKAKKPVFKSTVADSNTDNRY
RLAKIIVTVFAFATRFYLINDPKEVVFDEVHFGKFASYYLEGTYFFDLHPPFAKMLIA
FVGWLVGYDGAFKFENIGDSYIANRVPYVAYRSLSATLGALTVPIVFETLQECGFSVW
ACVIGSSLILFDNAHIAETRLILLDATLIISVALSLYAYVRFSKQRDSPFSRDWWTWL
LATGVSLSFVISTKYVGAFTFTTVGAAVLIDLWNLLDYRRGLSMKEFGKHFFARLFSL
IFVPLCIFLFWFWVHFAILTKSGPGDGFMSSKFQETLGDNILAKLAKQVNYYDEITIS
HKESPLFLSSGSIHYPLRYDDGRISSQGQLVSTQELVNGAPTNDQIWQIVPVVDFPEG
HREGIPIETETQVRLKHVSTNSFLLAHDVASPYYPTNEEFTTASFEEAEGPRFNDTLF
EIRSVTGEKDILKTKGSFFRLIHVPTRVAMWTHDDKQLPAEWASGHYEVNGVKNVHER
TATWFVDEIVNLTDPERLKFTPRAPRKMNFFRKYLELQYTMFAQNNALTSSHPYASEP
ITWPFLVRGVSFWTKDTERRQIYFIGNFIGWWIEAAMLAIYAGVVVADQLTRRRAMYP
INDVARSKIYNSLGFFFIAWATHYFPFFLMGRQKFLHHYLPAHLAAALLTGGLYDFVF
CELDDEAVHAAQSGKKRSNWRLKIVSVISLVGIVICFAYFSPLTYGNTPLTPAEVRAR
QWMDIELHFAK"
gene <569415..>570353
/gene="TFB3"
/locus_tag="AWJ20_4794"
/db_xref="GeneID:30036964"
mRNA <569415..>570353
/gene="TFB3"
/locus_tag="AWJ20_4794"
/product="TFIIH/NER complex subunit TFB3"
/transcript_id="XM_018881889.1"
/db_xref="GeneID:30036964"
CDS 569415..570353
/gene="TFB3"
/locus_tag="AWJ20_4794"
/inference="similar to AA sequence:KEGG_Orthology:K10842"
/note="Subunit of TFIIH and nucleotide excision repair
factor 3 complexes; involved in transcription initiation,
required for nucleotide excision repair; ring finger
protein similar to mammalian CAK and TFIIH subunit;
GO_component: GO:0070985 - TFIIK complex [Evidence IDA]
[PMID 19818408]; GO_component: GO:0005675 - holo TFIIH
complex [Evidence IDA] [PMID 19818408]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_function:
GO:0000990 - core RNA polymerase binding transcription
factor activity [Evidence IC] [PMID 19818408];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0008270 - zinc ion binding [Evidence
IEA]; GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0006289 - nucleotide-excision repair
[Evidence IMP] [PMID 10681587]; GO_process: GO:0070816 -
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 19450536]; GO_process: GO:0070816 -
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 19679665]; GO_process: GO:0006355 -
regulation of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0006366 - transcription from RNA polymerase
II promoter [Evidence IDA] [PMID 19818408]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="TFIIH/NER complex subunit TFB3"
/protein_id="XP_018736323.1"
/db_xref="GeneID:30036964"
/translation="MCPICKSSRYLNPDMRFLVNPECYHKICESCVDRIFSVGPAQCP
YAGCEKILRKNKFKTQVFEDLGVEREVDIRARVSRTFNKRQQDFDSLDDYNDYLEEVE
NIIFNLVNGVDVEATESKLQAYEQANRNIILANSLRQKQEDEQMEELQSLETQRRRKA
QLLALQAEEDERRIKEQTEKELVRQLATGEGDAAAIMKNVSNMALKRSSARRRQLEEE
LRQPLSIPLRLRGARSATPGNATPFTPFNGDRQQEYLFTVNDEYFDPLMNDVANNPQY
RASGFSVKGSFRQALVSAFFGIGCDVQHEKAAPLAV"
gene <571975..>572736
/gene="RCR2"
/locus_tag="AWJ20_4795"
/db_xref="GeneID:30036965"
mRNA <571975..>572736
/gene="RCR2"
/locus_tag="AWJ20_4795"
/product="Rcr2p"
/transcript_id="XM_018881890.1"
/db_xref="GeneID:30036965"
CDS 571975..572736
/gene="RCR2"
/locus_tag="AWJ20_4795"
/note="Vacuolar protein; presumably functions within the
endosomal-vacuolar trafficking pathway, affecting events
that determine whether plasma membrane proteins are
degraded or routed to the plasma membrane; RCR2 has a
paralog, RCR1, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0000324 -
fungal-type vacuole [Evidence IDA] [PMID 14562095];
GO_component: GO:0000324 - fungal-type vacuole [Evidence
IDA] [PMID 17287526]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISS]
[PMID 15590673]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_component: GO:0031982 - vesicle
[Evidence IDA] [PMID 17287526]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0016192 -
vesicle-mediated transport [Evidence IGI] [PMID 17287526]"
/codon_start=1
/product="Rcr2p"
/protein_id="XP_018736324.1"
/db_xref="GeneID:30036965"
/translation="MYLYLAEALLVRRDNNDDLVCDDEGDCYYNTPWYNYGRWLVVAA
IAIVFAVSIYLMRRNAANRLRGAMAPLSNRGWTWVPPTYHQSQRDYSNTTQAPPAAPP
YNRSLGTEDAGYYDSYGNFIQTGPVHTVAPPTGPPPPPSPLSPVDSSGVQIPPPVLHH
PSNYEMRDVYPSDRKSPSVFTYGPFPEDRYEDLSGPSNGSSSDPNHIIHHENSSNDPS
SAAGGPSFVELGGASVPPVDDPEHHLVPPPSQQHN"
gene complement(<573354..>574247)
/gene="FOL2"
/locus_tag="AWJ20_4796"
/db_xref="GeneID:30036966"
mRNA complement(<573354..>574247)
/gene="FOL2"
/locus_tag="AWJ20_4796"
/product="GTP cyclohydrolase I"
/transcript_id="XM_018881891.1"
/db_xref="GeneID:30036966"
CDS complement(573354..574247)
/gene="FOL2"
/locus_tag="AWJ20_4796"
/inference="similar to AA sequence:KEGG_Orthology:K01495"
/note="GTP-cyclohydrolase I; catalyzes the first step in
the folic acid biosynthetic pathway; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0005525 - GTP
binding [Evidence IEA]; GO_function: GO:0003934 - GTP
cyclohydrolase I activity [Evidence IEA,IEA]; GO_function:
GO:0003934 - GTP cyclohydrolase I activity [Evidence IMP]
[PMID 8573145]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0035998 - 7,8-dihydroneopterin
3'-triphosphate biosynthetic process [Evidence IEA];
GO_process: GO:0046656 - folic acid biosynthetic process
[Evidence IEA]; GO_process: GO:0009396 - folic
acid-containing compound biosynthetic process [Evidence
IMP] [PMID 8573145]; GO_process: GO:0008152 - metabolic
process [Evidence IEA]; GO_process: GO:0046654 -
tetrahydrofolate biosynthetic process [Evidence IEA]"
/codon_start=1
/product="GTP cyclohydrolase I"
/protein_id="XP_018736325.1"
/db_xref="GeneID:30036966"
/translation="MAHTEPSKLDLSAAAATNESAIVDSDSVPAGEHDSRSIVPSTVP
SHHRAPSGAPSGPVATAPNQYPKYIEHLTRLADARPSSPYLNPIDYDGLSWPSVGARE
RLESSVEEKAAREKRIADAVRTLLTELGEDPNRSGLLETPERYARAMLFFTKGYEDNV
KDVIKNALFEEDHDEMVIVKDIDVFSLCEHHLVPFYGKIHIGYIPNSRVIGLSKLARL
AEMFARRLQVQERLTKQVAMALWELLQPQGVAVVMEASHMCMVMRGVQKPGSSTTTSC
MLGRFREAQRTREEFLSLLKK"
gene complement(<577671..>578729)
/gene="HUA1"
/locus_tag="AWJ20_4797"
/db_xref="GeneID:30036967"
mRNA complement(<577671..>578729)
/gene="HUA1"
/locus_tag="AWJ20_4797"
/product="Hua1p"
/transcript_id="XM_018881892.1"
/db_xref="GeneID:30036967"
CDS complement(577671..578729)
/gene="HUA1"
/locus_tag="AWJ20_4797"
/note="Cytoplasmic protein containing a zinc finger
domain; sequence similarity to that of Type I J-proteins;
computational analysis of large-scale protein-protein
interaction data suggests a possible role in actin patch
assembly; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 11489916]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11914276]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008150 -
biological_process [Evidence ND]"
/codon_start=1
/product="Hua1p"
/protein_id="XP_018736326.1"
/db_xref="GeneID:30036967"
/translation="MSTNPFISHKPVPISNNTGGSSRNGDSRLSQYNTNGGSSRPNTS
TTGGDASSTLSIPRTETGSYSRSSIGSGNSESNSTSNHILPPEEEHPDEEPPPYTPEA
EPGMSVAGMSNIPPHIQAQISMATGNSQTSNNNFQNHAHNQSHVNNSHPQSQTYHPPP
HPPPPARPPRPTTASSSSPARPPRPQSSAFPGSNYHSYGQTNNSHNRPTTSQHQAYHH
RPGNANTNHPPPQNPNLPFTYPPGYYCYKCNNTGIKLKNGLTCQDCYGRFARQGRNVQ
VIHSGQPSMFNPLAPFMGPTTSYVPYYPGQPPPRVVRPGDPSIGGTLCGRCRGRGMIS
DFFGDETCPTCRGVGRLL"
gene <580480..>580551
/locus_tag="AWJ20_4798"
/db_xref="GeneID:30036968"
tRNA <580480..>580551
/locus_tag="AWJ20_4798"
/product="tRNA-Thr"
/db_xref="GeneID:30036968"
gene complement(<586370..>587032)
/locus_tag="AWJ20_4799"
/db_xref="GeneID:30036969"
mRNA complement(<586370..>587032)
/locus_tag="AWJ20_4799"
/product="hypothetical protein"
/transcript_id="XM_018881893.1"
/db_xref="GeneID:30036969"
CDS complement(586370..587032)
/locus_tag="AWJ20_4799"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736327.1"
/db_xref="GeneID:30036969"
/translation="MNIAEILNDDGEERIHNNASSRSTSPRSGSPVKRPRNSSDIEGG
SDPKRLADGSTSTLKSDSTPLQQSPSAAGPSESGNSNLNTRNSTGGLPRDIEPEPTAA
SSTIASGSSSSLASIPGPSIGSSVVKSEPTTEGAPVATVSGGESEGTPAADKKGKSKR
SKAKNSTWAIEDDRKLVDLVMSTLPTQDFAEYARVLNKRDAQTIRYRWKVILRRAKGE
GL"
gene complement(<595111..>595182)
/locus_tag="AWJ20_4800"
/db_xref="GeneID:30036972"
tRNA complement(<595111..>595182)
/locus_tag="AWJ20_4800"
/product="tRNA-Thr"
/db_xref="GeneID:30036972"
gene complement(<595202..>595273)
/locus_tag="AWJ20_4801"
/db_xref="GeneID:30036973"
tRNA complement(<595202..>595273)
/locus_tag="AWJ20_4801"
/product="tRNA-Thr"
/db_xref="GeneID:30036973"
gene complement(<595567..>597051)
/gene="UGA1"
/locus_tag="AWJ20_4802"
/db_xref="GeneID:30036974"
mRNA complement(<595567..>597051)
/gene="UGA1"
/locus_tag="AWJ20_4802"
/product="4-aminobutyrate transaminase"
/transcript_id="XM_018881896.1"
/db_xref="GeneID:30036974"
CDS complement(595567..597051)
/gene="UGA1"
/locus_tag="AWJ20_4802"
/inference="similar to AA sequence:KEGG_Orthology:K13524"
/note="Gamma-aminobutyrate (GABA) transaminase; also known
as 4-aminobutyrate aminotransferase; involved in the
4-aminobutyrate and glutamate degradation pathways;
required for normal oxidative stress tolerance and
nitrogen utilization; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 23740823];
GO_function: GO:0003867 - 4-aminobutyrate transaminase
activity [Evidence IEA,IEA]; GO_function: GO:0003867 -
4-aminobutyrate transaminase activity [Evidence IMP] [PMID
3888627]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0030170 - pyridoxal
phosphate binding [Evidence IEA]; GO_function: GO:0008483
- transaminase activity [Evidence IEA,IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
process [Evidence IMP] [PMID 3888627]; GO_process:
GO:0009448 - gamma-aminobutyric acid metabolic process
[Evidence IEA]"
/codon_start=1
/product="4-aminobutyrate transaminase"
/protein_id="XP_018736328.1"
/db_xref="GeneID:30036974"
/translation="MIRSVTRSSLLRNRVAGARLTRLSSSVAASYFPNEPTAPSVKTA
IPGPQTKAALEKLNKSMDIPAAYFVADYYKSLGNYIVDVDGNVLLDVYAQIASIALGY
NNPALIEAAKSDAMINSLVNRPATGNFPSNDYADILEKGIMAAAPKGFSNVWTALSGS
DANETAFKAAFIYQASKKRGSGGFTQEELTTTMSNQAPGSPAGSILSFESSFHGRLFG
SLSATRSKPIHKLDIPAFDWPKAPFPRLQYPLEANVEANKQEEKRCLEEFEKILTTWK
TPVAAVIVEPILSEGGDFHASRDFFQGIRDITKKHDVVMIVDEVQTGVGGTGKLWAHE
HWNLDSVPDIVTFSKKAQAAGYYFSNPELRPKLAYRQFNTWCGDPSKAIIASAIFNEI
KKNNLVERTAQVGDYLFGKLEALATKYPQNIANLRGKGQGTFIAWDCSSPEFRDNFIK
KAKELGVNIGGCGVQAIRLRPALTFEEKHADILLDVVEKIVASA"
gene complement(<598643..>601018)
/gene="SET2"
/locus_tag="AWJ20_4803"
/db_xref="GeneID:30036975"
mRNA complement(<598643..>601018)
/gene="SET2"
/locus_tag="AWJ20_4803"
/product="histone methyltransferase SET2"
/transcript_id="XM_018881897.1"
/db_xref="GeneID:30036975"
CDS complement(598643..601018)
/gene="SET2"
/locus_tag="AWJ20_4803"
/inference="similar to AA sequence:KEGG_Orthology:K11423"
/codon_start=1
/product="histone methyltransferase SET2"
/protein_id="XP_018736329.1"
/db_xref="GeneID:30036975"
/translation="MSKISRPIKVIEFSDSDDDLYGDKPSKQNGSHGPAENSVSSEKV
RVAAAPSKVLKKENNIKISTSKPKVEIIDISNDDKSDIDEDDEPVSGAGRRARGNGTV
TRSDLKQENTITDGDSAGSEEPVDPFDDQIKPKSEDKKNVKVTRQLFNDLESKTAEAQ
STYIQLEQCTYQGRGNIGSSGQVEAMNCDCREHWVNGKNIACGDDSDCINRLTSIECT
NDNCTCGDDCGNQRFQAKQYANVDVIQTELKGYGVRALEAIPQNTFIYEYIGEVIDES
NFRQRMETYKLEGIRHFYFMMLQKGEFIDATKQGSMARFCNHSCRPNCYVDKWIVGDK
VRMGIFSKRDIIKGEEITFDYNVDRYGTQAQPCYCGESNCIGILGGKTQTEAAATKLP
QLIVDVLDLTRQDEKQWLSIAKKFKKDGRDGKYATADASGASDEDLYREYSLSLPAKP
VQEESVTKIMSALLQGQEQWLIEKLVNRIFITDDSSVHGQVMRMHGYQVFSVILAADW
PNDVVPLMILEILTRWPRLTKNKISSSKIESIVAGLAKDSTSDKVQKLASTLLQEWSS
LEMAYRIPRRERGAKESTNSNSASGTPQEEGASSSPTRPAYPRAYGSNSHDNIQDNRG
EKLPRRDWGAKDNYNNRGRERDYDNRYRRNRTRSPSPLPSGWETALAPDGRRYYFNRT
TGLSSWTLPKEGSPQKDSRIIPDNEREKAELLQKEQERLQIEQIIESANQSRQNIVLL
QQQKINELQSIVDAVKTKSSHRSSHRPSSEKKKKREDDTPESVFTRTVSST"
gene complement(<601398..>602336)
/locus_tag="AWJ20_4804"
/db_xref="GeneID:30036976"
mRNA complement(<601398..>602336)
/locus_tag="AWJ20_4804"
/product="hypothetical protein"
/transcript_id="XM_018881898.1"
/db_xref="GeneID:30036976"
CDS complement(601398..602336)
/locus_tag="AWJ20_4804"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736330.1"
/db_xref="GeneID:30036976"
/translation="MSCPPPWTPLLDSCLKAVEKLPTFTFCTVSHNRIPHARTCVSRG
WLFDDKSTGVLVFTTDMRMQKVSDLNETNDAYEACFYFPNKSTQIRLSGFAQLLSPNH
TPQLSSTLPLSQPVKSSPPSSGSSSRSGSPSGNPGSPNPNNSSAPVAVPDSSSNNTAN
NEAKGTSSSPSETILAPLASAATSRKHLPRSYYPVITPSYDPSTDYDSIFPPPTAEEW
LNEYNRLWSSMSPRMKKTFKYPTPKSLLDGSTTKALDSISRGVDGASEDSGKEHFVVV
LMLVNQVDIVVDESIGRRTLFTRVRYDEWKEQDLSP"
gene complement(<604056..>604766)
/gene="ARC1"
/locus_tag="AWJ20_4805"
/db_xref="GeneID:30036977"
mRNA complement(<604056..>604766)
/gene="ARC1"
/locus_tag="AWJ20_4805"
/product="Arc1p"
/transcript_id="XM_018881899.1"
/db_xref="GeneID:30036977"
CDS complement(604056..604766)
/gene="ARC1"
/locus_tag="AWJ20_4805"
/inference="similar to AA sequence:KEGG_Orthology:K15437"
/note="Protein that binds tRNA and methionyl- and
glutamyl-tRNA synthetases; involved in tRNA delivery,
stimulating catalysis, and ensuring localization; also
binds quadruplex nucleic acids; protein abundance
increases in response to DNA replication stress;
methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA
synthetase is Gus1p; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11726524]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 15710377]; GO_component:
GO:0017102 - methionyl glutamyl tRNA synthetase complex
[Evidence IMP,IPI] [PMID 9659920]; GO_function: GO:0003677
- DNA binding [Evidence IEA]; GO_function: GO:0003723 -
RNA binding [Evidence IEA]; GO_function: GO:0008047 -
enzyme activator activity [Evidence IDA] [PMID 11069915];
GO_function: GO:0080025 -
phosphatidylinositol-3,5-bisphosphate binding [Evidence
IDA] [PMID 16343487]; GO_function: GO:0032266 -
phosphatidylinositol-3-phosphate binding [Evidence IDA]
[PMID 16343487]; GO_function: GO:0000049 - tRNA binding
[Evidence IEA,IEA]; GO_function: GO:0000049 - tRNA binding
[Evidence IMP,IPI] [PMID 11726524]; GO_process: GO:0051351
- positive regulation of ligase activity [Evidence IDA]
[PMID 11069915]; GO_process: GO:0006418 - tRNA
aminoacylation for protein translation [Evidence IMP]
[PMID 9659920]"
/codon_start=1
/product="Arc1p"
/protein_id="XP_018736331.1"
/db_xref="GeneID:30036977"
/translation="MALCLGRTCLKHTFKTSASYSVKYSVFRVNTNQNIRLPDWPLKS
SIRTYSTENQPKTPNPSVVQFKTAVVEKVDYHPNADSLYVSQVRVEGGPTSELLTVCS
GLVKYVPKDELEGRRIVVVTNLKPSKLRGIKSEAMLLAAVGDDSAGNKKLELVSPPKQ
AALGETLFFDKYPISQSQQVPLKSIKSKVWQEVQKQLFVNVSHEVIFRDHELGDLVLR
NDAGDPATVASLTNGTVS"
gene <605389..>608388
/locus_tag="AWJ20_4806"
/db_xref="GeneID:30036978"
mRNA <605389..>608388
/locus_tag="AWJ20_4806"
/product="hypothetical protein"
/transcript_id="XM_018881900.1"
/db_xref="GeneID:30036978"
CDS 605389..608388
/locus_tag="AWJ20_4806"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736332.1"
/db_xref="GeneID:30036978"
/translation="MLGRLLSHAFTSPDGSSSPDGGRFSRASSPNFVSIGDGANDDEI
HPTFPVSFLDEADTRNLLYGQSFTPAGILSLDVLKDIRVVVICDSSCVGGFIYDSARE
VTASGSVNTNNSVSGGKTSITPHRGSLDHRTNSPSLVASTLSSVSALTRSFSSTALSS
AAAINNLSGLGIGLGQTDSTTANPDGVTSGKGLGDSSPSNKPLARPNTIASPQISSGR
VSAPNSPHRRKSSGNNLPTIAPDVAPILQLALLKDLVFGYSAMKFQGQLTKIHPLPRS
KGSSSKKQPWIVSRIFKIAVDDPSLNRTDPVNRQSTTMRVDSAPKQFLTSTGSTISNA
SVDTNNTDLTVDDADNENDSATDGLPGWQPKSNSTVPPLPPHTKDVRCAICISFNLPP
ESEDAITNHWTELSYALADLQATIYRKLERQLPQVVLDSRRKKHTPIMSGGHGSGLNH
DSRFSGGRVLYSIDGDLEIQNAVDFFKSRFSTALRIPRVLCGQDNWSDLYSEMCIAAK
KWEGTSDGGFLGAMICCYIDFNKDLLGGLIHEDDDIPKRTIILANNRIVSRKLIFILS
SLIRSRTSERIRKRISLANSLNDSFVKTHTQRSSSVSTTYSVGTNSPRTRTSAKFSFV
DGGWEVSQPASMIEPVGTVAIPHVVRPSFSSSSLAHSVGYGSTSDALSSSINGRNSRN
SGGFFSNLWNSDNSYPETPGTPYDDYYFHEQLANADRDMASSFQSKNFTIGDTTNGTV
TPSLIRPSMSRCHSSERSATTLNTINHRLYHRQHTGESNISANNPHTSLHQRTLLQNA
SDNDLTNLSYLPTAPPYRLVHDEENQIVIDVSESATPDQPYEDLRPIVLPRVAGHVSN
FHPDFILQACPITRDLEDQISRTMLRDGDVNSVSIAARNSISSVPSKRQALSKSLIIN
LRTREISTYTLVASTDETSNMTQKLVRKVVFANHKPSPGFSTAFANISKQIADIVEGE
EGTIHSPNRGAYFTSLVKAFEGCFGLL"
gene complement(<608637..>609689)
/gene="TFB4"
/locus_tag="AWJ20_4807"
/db_xref="GeneID:30036979"
mRNA complement(<608637..>609689)
/gene="TFB4"
/locus_tag="AWJ20_4807"
/product="TFIIH/NER complex subunit TFB4"
/transcript_id="XM_018881901.1"
/db_xref="GeneID:30036979"
CDS complement(608637..609689)
/gene="TFB4"
/locus_tag="AWJ20_4807"
/inference="similar to AA sequence:KEGG_Orthology:K03143"
/note="Subunit of TFIIH complex; involved in transcription
initiation, similar to 34 kDa subunit of human TFIIH;
interacts with Ssl1p; GO_component: GO:0000439 - core
TFIIH complex [Evidence IEA]; GO_component: GO:0000439 -
core TFIIH complex [Evidence IDA] [PMID 14500720];
GO_component: GO:0000439 - core TFIIH complex [Evidence
IDA] [PMID 19818408]; GO_component: GO:0005675 - holo
TFIIH complex [Evidence IDA] [PMID 19818408];
GO_component: GO:0000112 - nucleotide-excision repair
factor 3 complex [Evidence IPI] [PMID 10681587];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0000990 - core RNA polymerase binding
transcription factor activity [Evidence IC] [PMID
19818408]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_process: GO:0006281 - DNA repair
[Evidence IEA]; GO_process: GO:0006974 - cellular response
to DNA damage stimulus [Evidence IEA]; GO_process:
GO:0006289 - nucleotide-excision repair [Evidence IEA];
GO_process: GO:0006289 - nucleotide-excision repair
[Evidence IMP] [PMID 10506223]; GO_process: GO:0070816 -
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 19450536]; GO_process: GO:0070816 -
phosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 19679665]; GO_process: GO:0006355 -
regulation of transcription, DNA-templated [Evidence
IEA,IEA]; GO_process: GO:0006366 - transcription from RNA
polymerase II promoter [Evidence IDA] [PMID 19818408];
GO_process: GO:0006351 - transcription, DNA-templated
[Evidence IEA]"
/codon_start=1
/product="TFIIH/NER complex subunit TFB4"
/protein_id="XP_018736333.1"
/db_xref="GeneID:30036979"
/translation="MNAVDDTHASLGGSERTGSDEHPTLLGIVLDTNPTAWAELENKL
SLKEAVESLLIFINAHLSLNNANRVAVIAAHTTGAKFLYPKPPSSSFENGSQSGHTSL
KAMSGTTGTANIFDRQTMYRQFREVDEAVVGELDRLVSSATEKSVRSTQSAVSGALSL
ALTYINRVNSNEDEVKTHARILLVSVSGDLSSQYIPTMNCIFAAQKKRIPIDVCKLAG
DTVFLQQASDSTNGTYMKLDHPRGLVQYLLSAFLVDPAILPHVNLSTQRDVDFRAACF
VTKTVVDIGCVCSVCLCIMSKVPADGSCPMCGTVYDPQALQKINRKPMVIVKKKPKKK
KAKLDSTPVGTPSPIP"
gene complement(<610680..>611036)
/gene="MRPL37"
/locus_tag="AWJ20_4808"
/db_xref="GeneID:30036980"
mRNA complement(<610680..>611036)
/gene="MRPL37"
/locus_tag="AWJ20_4808"
/product="mitochondrial 54S ribosomal protein YmL37"
/transcript_id="XM_018881902.1"
/db_xref="GeneID:30036980"
CDS complement(610680..611036)
/gene="MRPL37"
/locus_tag="AWJ20_4808"
/inference="similar to AA sequence:KEGG_Orthology:K17435"
/note="Mitochondrial ribosomal protein of the large
subunit; GO_component: GO:0005762 - mitochondrial large
ribosomal subunit [Evidence IDA] [PMID 2060626];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IDA] [PMID 2060626]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IC] [PMID 2060626];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="mitochondrial 54S ribosomal protein YmL37"
/protein_id="XP_018736334.1"
/db_xref="GeneID:30036980"
/translation="MLRLITRQVLRTTVPRTSFVRLASTSAPNTETTSSTKKTITSIA
PAGTKLKGLNIKKGGEDPIALAENEYPEWLWELLDPAAQKAKLESDAGRQARKERRSA
NRQKIKMNNFLAGMKN"
gene <614858..>617515
/gene="LAS21"
/locus_tag="AWJ20_4809"
/db_xref="GeneID:30036981"
mRNA <614858..>617515
/gene="LAS21"
/locus_tag="AWJ20_4809"
/product="Las21p"
/transcript_id="XM_018881903.1"
/db_xref="GeneID:30036981"
CDS 614858..617515
/gene="LAS21"
/locus_tag="AWJ20_4809"
/inference="similar to AA sequence:KEGG_Orthology:K05310"
/note="Integral plasma membrane protein; involved in the
synthesis of the glycosylphosphatidylinositol (GPI) core
structure; mutations affect cell wall integrity;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0030176 -
integral component of endoplasmic reticulum membrane
[Evidence IDA] [PMID 10329735]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0005887 -
integral component of plasma membrane [Evidence IDA] [PMID
10329735]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0051377 -
mannose-ethanolamine phosphotransferase activity [Evidence
IMP] [PMID 15452134]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IMP] [PMID
10329735]; GO_process: GO:0006506 - GPI anchor
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0006506 - GPI anchor biosynthetic process [Evidence
IMP] [PMID 10329735]; GO_process: GO:0006506 - GPI anchor
biosynthetic process [Evidence IMP] [PMID 15452134];
GO_process: GO:0008152 - metabolic process [Evidence IEA]"
/codon_start=1
/product="Las21p"
/protein_id="XP_018736335.1"
/db_xref="GeneID:30036981"
/translation="MNTSVRKWILIGVILCLQLGGFGLFARGFFPKKLVLPGEGVFFS
DENGMLSVDELEPRFDKLILVVIDALRSDFIFSSGRSAMSEVHRLIRDGAGFPFTAHS
TPPTVTLPRIKGLTTGSTPNFLDAILNIAESDTSSTLANQDSWVNQIQKQGKRLHMFG
DDTWIKLFPRAFEKFDGTASFFVSDYTEVDNNVTRHIDYELGPGRESWDTLILHYLGL
DHIGHKGGPDSVFMPEKQREMDHIIHKLYENMEENTLLVVTGDHGMNEAGNHGGSSSG
ETSAGMVFLSPKFVQIKNSSQEGNNGHKAPLDNPTEGEFTYYSRIQQTDLVPTLASLL
KFPIPRNSLGVVIPDILELWENGEKAIILEQNVKQFVHVMASTYPGFDKIDVDIEMKC
QAAQDSVELGEVDELRCLWWRLKSGGREIYGDKDLVFEFLYGCQDLLSKAASNYVERD
LQFGLGLVILSAIVAISAVFLLKFSANLKIVLIIIAGVFSASMFGSSLVEEEHHFWYW
GATGWLSWLYITSSRRKFRDGGNWIACLMLIRIIRGWNQTGQKYAGGPDTAKWLGQDS
LGDNMGMESGAGQVLWGLVLVQYASLLGKLWLGGFSDVSSLAGFVFSFCIAMSSLVFK
INMAIQSGEFVPKYLQFIVVSADDSEGLINLARLSFFTIGLASLYELSKLILAPRDNR
VAVSSSLTNLSYIFEAFLVTQTRTVNIPLFIFFHLLRTYLARAYLINHRSSRDIVIII
TIFTLILQHVSFFAMGNSNSLASIDLSNAYNGISSYQIVLVGVLTFVNNWVGPLYWTV
AGLTMLIDSGIIPQTTTKAQIISNKILISQMFFSIATAGILGACYVLKHHLFIWTVFS
PKLLYSAAWLILQHGLIDIMASTALAYLF"
gene <619169..>620080
/gene="TAF5"
/locus_tag="AWJ20_4810"
/db_xref="GeneID:30036983"
mRNA <619169..>620080
/gene="TAF5"
/locus_tag="AWJ20_4810"
/product="Taf5p"
/transcript_id="XM_018881905.1"
/db_xref="GeneID:30036983"
CDS 619169..620080
/gene="TAF5"
/locus_tag="AWJ20_4810"
/inference="similar to AA sequence:KEGG_Orthology:K13124"
/note="Subunit (90 kDa) of TFIID and SAGA complexes;
involved in RNA polymerase II transcription initiation and
in chromatin modification; GO_component: GO:0000124 - SAGA
complex [Evidence IDA] [PMID 9674426]; GO_component:
GO:0046695 - SLIK (SAGA-like) complex [Evidence IDA] [PMID
12446794]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA,IEA]; GO_component: GO:0005669 - transcription
factor TFIID complex [Evidence IDA] [PMID 10788514];
GO_component: GO:0005669 - transcription factor TFIID
complex [Evidence IDA] [PMID 15448131]; GO_function:
GO:0001075 - RNA polymerase II core promoter
sequence-specific DNA binding transcription factor
activity involved in preinitiation complex assembly
[Evidence IC] [PMID 15448131]; GO_function: GO:0003682 -
chromatin binding [Evidence IDA] [PMID 12600943];
GO_function: GO:0032947 - protein complex scaffold
[Evidence IMP] [PMID 11585915]; GO_function: GO:0043130 -
ubiquitin binding [Evidence IDA] [PMID 21070969];
GO_process: GO:0051123 - RNA polymerase II transcriptional
preinitiation complex assembly [Evidence IC] [PMID
10788514]; GO_process: GO:0016568 - chromatin modification
[Evidence IDA] [PMID 9674426]; GO_process: GO:0016573 -
histone acetylation [Evidence IDA] [PMID 9674426];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA,IEA]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IDA] [PMID 12138208]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IDA] [PMID 15448131]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Taf5p"
/protein_id="XP_018736336.1"
/db_xref="GeneID:30036983"
/translation="MSVKLKIPHAHTGAIHALSYNSNGSYVLSGGQDRKVVLWNANSG
VKVQVYQFHAYEILDINVTFDDTRFVSCGGDRTAYVCDVSSGKTTNRFTGHIARINSA
CFNFDSSVVATASYDKTVKMWDCRSSSHSPIQTLTDASDSVSSVVIDRAQVFTGSVDG
KVRIYDIRKGKLSTMTIGYPVTSVKPNVDGDTVLVSSLDSRIRLMDVADGNLLKTYKG
HVNMLYRIKSAFVKNESEVVTCSEDGKIYFWDIESGEIERTLTQTSQLNSNSTMSSTS
YSLAIHPTTNEISTSSLDGSIQLWNKF"
gene <620369..>621115
/gene="MRP20"
/locus_tag="AWJ20_4811"
/db_xref="GeneID:30036984"
mRNA <620369..>621115
/gene="MRP20"
/locus_tag="AWJ20_4811"
/product="mitochondrial 54S ribosomal protein YmL41"
/transcript_id="XM_018881906.1"
/db_xref="GeneID:30036984"
CDS 620369..621115
/gene="MRP20"
/locus_tag="AWJ20_4811"
/inference="similar to AA sequence:KEGG_Orthology:K02892"
/note="Mitochondrial ribosomal protein of the large
subunit; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005762 - mitochondrial
large ribosomal subunit [Evidence IDA] [PMID 1544898];
GO_component: GO:0005762 - mitochondrial large ribosomal
subunit [Evidence IDA] [PMID 9151978]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA,IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IEA]; GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IDA] [PMID 1544898]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IDA] [PMID 9151978]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IMP] [PMID 1544898];
GO_process: GO:0032543 - mitochondrial translation
[Evidence IC] [PMID 9151978]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="mitochondrial 54S ribosomal protein YmL41"
/protein_id="XP_018736337.1"
/db_xref="GeneID:30036984"
/translation="MPRRIPKSIKRSSRAKPPTPYIPKQVKFPLSKKAQAKPTPKGPS
YSERIFNKLQESIEGDGPHFPVGGREVYFPHAKVVLVRPSAKHTPYQAKFVVPRYFNK
LDLRDYLYHVYGLKALNITTQLLWARWKRDAPGKPRYRTPQVKKMTIEMADPFVWPKP
TTLQEIRKHYNVNFIKELHKYGEDANRFGSDKLKPPRAFGGIAGPYVPPASPFLPKQI
TRQMLKKKKVAEVQALRQKDVDMVKNFLRL"
gene complement(<621264..>625349)
/locus_tag="AWJ20_4812"
/db_xref="GeneID:30036985"
mRNA complement(join(<621264..624717,625288..>625349))
/locus_tag="AWJ20_4812"
/product="hypothetical protein"
/transcript_id="XM_018881907.1"
/db_xref="GeneID:30036985"
CDS complement(join(621264..624717,625288..625349))
/locus_tag="AWJ20_4812"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736338.1"
/db_xref="GeneID:30036985"
/translation="MASLRFSAESNEELIVTAPRCSLRSVSKSIEALLSSKASATGSG
SGAGDGAGTGSTDVGTGSSTSYSLDTETAENIQLYIEKHPPDPAEATRLHDELVRIHK
AYVLPESHPDSELYFVRCLIDFVPCLINTDSIEEWFGRYSGPAIDSAGHRNALVKLSR
EFLLALLLVSTNNNMGSLSDQDNESASKIKSIYLANSRKVAQMILAQYLKGDDGDYSF
TSNVDNMLKGKEGMKSEERRRFAKSNAQNILIQYGMKAPVEFLTVVSDRFGESKLRIQ
LLALLSSYVSQQPPYLFQIRSTPLLDNLYQCLIRDKSSFGLSICATVVAMILPHICDI
VVDQLPKLFAIYGRLCSWQLELGSEEDDADAASDEDDEKHSKDETCTTNSSDNWQVLN
NLIDIQDARGPIITPLFTFLYGLFPVNLICFIRQPNEYLSESGYKRPFDDFWDKHYIQ
ELSRPCLELHTLNPSMISMTREQELTDKTRWQFMGSAYDIASLCLSLYNPRQDFNKRP
SHQVVSISSTSSRPQFRLPFEEVNNLIDPESQQNADGEIISASNLTSDYDTPELVNPA
VKRLSVVNVPPSILSRTSSSFDPTATGGPIREDSLTSPSTATNGRNSFLSPGLPSVEA
LLSDHEKLYSRREILPTLEKRDSMSAIPDELRLDDVVSPTDPGPSTANNSVHNLSVDV
NNNKPGTNNNNSTNSSLNTLRRGRSGTISSPAFIPTSQNTTSSAIHSPLLLSQPNQVA
AAAASSIAAASANVSAPGTGTTSSPNIPISSPTTGPQSAAIVSHEQLEKQFNLTIAFY
QRELLLIQNELEFVSFVEHQTQHRFSELRQKLKDSILANAQVDKLITQNKALKAALEK
PELEAAKYQKSLKTYKSERQAYEGSLVQRNKDFRTQINNLNQQLEQVKSELEQITTEK
KALLESVKSQEITISKMELDLTAAKERTVLFDSLQKAFEELKQKVEVLERQQENTDKS
NPQVDVVQGQLEQLRLAYHGLEADKDKIVTAYDKRIEELEKLVADYETRMNRNNGPPS
RESSDISQFIESFKKASEVRYNQLNTAYNELVDRFEDLSVRFREVLLNDEEHKARIDQ
PVLPKRSLLGYEMVRAEEENVSESNGVTKNIAAQPPQHPHPLVRNTTETRIRGRGGVQ
NTSNAPVPNSPGSTQSKRRPFRGYG"
gene <625102..>626079
/locus_tag="AWJ20_4813"
/db_xref="GeneID:30036986"
mRNA <625102..>626079
/locus_tag="AWJ20_4813"
/product="hypothetical protein"
/transcript_id="XM_018881908.1"
/db_xref="GeneID:30036986"
CDS 625102..626079
/locus_tag="AWJ20_4813"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736339.1"
/db_xref="GeneID:30036986"
/translation="MSYSNLDLINQVDSVPYTPQPVDAYYEFRSHDSKAVLGYILPEV
AKKLNYPDTFTVTGPTANQRGAVTINSSLDSAEKRSEAIAKVLEDLKENKTFQVLQGW
RNELYTVYNPTHVPYFLIERAGSGLFGIVTYGVHLVGYQPATETSPLKIWVPRRSYTK
ATYPGMLDNTIAGGIGYPCGVHETAIKEAYEEAGLDEEFMEKNLRPAGVVTYFHQVNN
HFFDSEASSRSTTTKDEATYQPEVEFIYDVIMAGDVKPRPVDGEVAEFYLMDVETVKQ
ELAKGSFKYNCALVMIDFFIRHGILTYENEPDFVNIAQRCHRVLEFPMK"
gene <627019..>628272
/gene="KTR4"
/locus_tag="AWJ20_4814"
/db_xref="GeneID:30036987"
mRNA <627019..>628272
/gene="KTR4"
/locus_tag="AWJ20_4814"
/product="Ktr4p"
/transcript_id="XM_018881909.1"
/db_xref="GeneID:30036987"
CDS 627019..628272
/gene="KTR4"
/locus_tag="AWJ20_4814"
/inference="similar to AA sequence:KEGG_Orthology:K03854"
/note="Putative mannosyltransferase involved in protein
glycosylation; member of the KRE2/MNT1 mannosyltransferase
family of type II membrane proteins with a short
cytoplasmic N-terminus, a membrane-spanning region and a
highly conserved catalytic lumenal domain; GO_component:
GO:0005794 - Golgi apparatus [Evidence ISS] [PMID
7975899]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA,IEA]; GO_function: GO:0000030 -
mannosyltransferase activity [Evidence IEA]; GO_function:
GO:0000030 - mannosyltransferase activity [Evidence ISS]
[PMID 7975899]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_function: GO:0016757 -
transferase activity, transferring glycosyl groups
[Evidence IEA]; GO_process: GO:0097502 - mannosylation
[Evidence IEA]; GO_process: GO:0006487 - protein N-linked
glycosylation [Evidence ISS] [PMID 7975899]; GO_process:
GO:0006486 - protein glycosylation [Evidence IEA]"
/codon_start=1
/product="Ktr4p"
/protein_id="XP_018736340.1"
/db_xref="GeneID:30036987"
/translation="MLVRYLRRPIAIIGLFSVVVLLAMSSYLFDLDLTPYYDRKAYGQ
KVAQGDFIPDKQVDLSKGYSAEDPSRNEDAKNPLFDEKLEPDGGLSRLTETDKRVPAT
LMSLVRNKEADDLMATIVQIESAFNNKFNYPWTFFNDEEFTEDFKTSALAATGGNCTF
VKIEPEDWMEPSWIDVDKARELGIKMKNKDDVQYADLPSYHRMCRWNSGRFFMNPALD
NYDYYWRVEPKTDFYCDIDYDIFAYMKENDKVYGFVINLYDSPQSIRTLWPTTKEFFE
QNPEYLHPNNALQWVVNTGREGHTKKANGYSTCHFWSNFEIGSLEFFRSKPYQDYFDF
LDKNGGFFYERWGDAPVHSLGLAMMADKSKIHYFKDIGYQHFPYFNCPASPKCKGCDA
GKFTGIDSLNTESCMSEWFKAAGTG"
gene complement(<628441..>629946)
/gene="AIP1"
/locus_tag="AWJ20_4815"
/db_xref="GeneID:30036988"
mRNA complement(<628441..>629946)
/gene="AIP1"
/locus_tag="AWJ20_4815"
/product="Aip1p"
/transcript_id="XM_018881910.1"
/db_xref="GeneID:30036988"
CDS complement(628441..629946)
/gene="AIP1"
/locus_tag="AWJ20_4815"
/note="Actin cortical patch component; interacts with the
actin depolymerizing factor cofilin; inhibits elongation
of aged ADP-actin filaments decorated with cofilin to
maintain a high level of assembly-competent actin species;
required to restrict cofilin localization to cortical
patches; contains WD repeats; protein increases in
abundance and relocalizes from cytoplasm to plasma
membrane upon DNA replication stress; GO_component:
GO:0030479 - actin cortical patch [Evidence IEA];
GO_component: GO:0030479 - actin cortical patch [Evidence
IDA] [PMID 10366597]; GO_component: GO:0005884 - actin
filament [Evidence IDA] [PMID 23333351]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
22842922]; GO_component: GO:0005856 - cytoskeleton
[Evidence IEA,IEA]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 19053807];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11914276]; GO_function: GO:0003779 - actin binding
[Evidence IEA]; GO_function: GO:0003779 - actin binding
[Evidence IPI] [PMID 7719850]; GO_process: GO:0030042 -
actin filament depolymerization [Evidence IGI,IPI] [PMID
10366597]; GO_process: GO:0051016 - barbed-end actin
filament capping [Evidence IMP] [PMID 23333351];
GO_process: GO:0006970 - response to osmotic stress
[Evidence TAS] [PMID 10652251]"
/codon_start=1
/product="Aip1p"
/protein_id="XP_018736341.1"
/db_xref="GeneID:30036988"
/translation="MVAKGEYQVIQGRINDLAWDADSQRIIAVGDGKEKYGHCFTADS
GNTVGSIAGHSAVVNAVSIRPVRPYRAVTVGDDARLVFYQGPPFQFSLSHQDKHTNFV
QDAQFSPNGEFIVSVGSDRKIALYEGKTGEFLSFVEDESNGHKGSIFSVAWSPDSSKF
ATASADQTVKLWSVADKKVLKTWSFPAGLENHQVGVVFAGEYIISLSYSGDLNYLQES
SDKPVKTVTGHQKTITSLASSGPSKPLVSGSYDGKIVHWDASDITASSSVHSHDGPVV
GIVSDGEHLWSVGWDDTLKRVGSDSSVSLFGQPKAVASGSDGIIAVVGESYIQVFDNG
KQLAFNTSLEFPAQAVSVSKSLVAVGSSDNSIHLFNRDLSVSNSVKLAPLRSAVTYLA
FSPDGEYLAAGDASGKITLYSIPDQSVVTGRWAFHTSRVTSIHWHPSGSHVVAGSADT
NIIIYSVANPGKNIKYLGAHKDGINSVLWTSDSTFASAGNDATVKRWRVTL"
gene complement(<634464..>634883)
/gene="DAL5"
/locus_tag="AWJ20_4816"
/db_xref="GeneID:30036989"
mRNA complement(<634464..>634883)
/gene="DAL5"
/locus_tag="AWJ20_4816"
/product="allantoate permease"
/transcript_id="XM_018881911.1"
/db_xref="GeneID:30036989"
CDS complement(634464..634883)
/gene="DAL5"
/locus_tag="AWJ20_4816"
/note="Allantoate permease; ureidosuccinate permease; also
transports dipeptides, though with lower affinity than for
allantoate and ureidosuccinate; expression is constitutive
but sensitive to nitrogen catabolite repression;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0015124 -
allantoate transmembrane transporter activity [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0042936 -
dipeptide transporter activity [Evidence IMP] [PMID
16429164]; GO_function: GO:0042936 - dipeptide transporter
activity [Evidence IGI,IMP] [PMID 17693598]; GO_function:
GO:0005215 - transporter activity [Evidence IEA];
GO_process: GO:0015719 - allantoate transport [Evidence
IMP] [PMID 3275614]; GO_process: GO:0042938 - dipeptide
transport [Evidence IMP] [PMID 16429164]; GO_process:
GO:0042938 - dipeptide transport [Evidence IGI,IMP] [PMID
17693598]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA,IEA]; GO_process: GO:0042939 -
tripeptide transport [Evidence IMP] [PMID 16429164]"
/codon_start=1
/product="allantoate permease"
/protein_id="XP_018736342.1"
/db_xref="GeneID:30036989"
/translation="MSASFLAFSSNHSVQLAGYCLFSMSPLCFICYLSTISSNTAGHT
KKVIFSATTLVGYCVGNLIGPQTFIASESPTYKTAKTTIVACYGVSLILIAATYYINR
RANRIRDEKNEKLPDDFVNSEFADLTDFQNPEFRYAL"
gene complement(<634933..>636057)
/gene="DAL5"
/locus_tag="AWJ20_4817"
/db_xref="GeneID:30036990"
mRNA complement(<634933..>636057)
/gene="DAL5"
/locus_tag="AWJ20_4817"
/product="allantoate permease"
/transcript_id="XM_018881912.1"
/db_xref="GeneID:30036990"
CDS complement(634933..636057)
/gene="DAL5"
/locus_tag="AWJ20_4817"
/note="Allantoate permease; ureidosuccinate permease; also
transports dipeptides, though with lower affinity than for
allantoate and ureidosuccinate; expression is constitutive
but sensitive to nitrogen catabolite repression;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0015124 -
allantoate transmembrane transporter activity [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0042936 -
dipeptide transporter activity [Evidence IMP] [PMID
16429164]; GO_function: GO:0042936 - dipeptide transporter
activity [Evidence IGI,IMP] [PMID 17693598]; GO_function:
GO:0005215 - transporter activity [Evidence IEA];
GO_process: GO:0015719 - allantoate transport [Evidence
IMP] [PMID 3275614]; GO_process: GO:0042938 - dipeptide
transport [Evidence IMP] [PMID 16429164]; GO_process:
GO:0042938 - dipeptide transport [Evidence IGI,IMP] [PMID
17693598]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA,IEA]; GO_process: GO:0042939 -
tripeptide transport [Evidence IMP] [PMID 16429164]"
/codon_start=1
/product="allantoate permease"
/protein_id="XP_018736343.1"
/db_xref="GeneID:30036990"
/translation="MITEHKANVSKDLGVVEKNVEVEIAEVKDVNLKGADLAMEVAFQ
SYGMEIDEATNKRILRKIDMYVCTLMCVVYSIQFLDKVTNSFAAIMGLIPDLHMTGSE
YSWIGSAFYLGYLVLEFPAAYLLQRFPIAKTVGIFVILWGFVLCMTSLPTTFGGFLTT
RIFLGMLESAVTPAFVLITSQWYKSSEQFTRTAFWVGCNGIGGMIGAAIAYGLAKRQM
AGDLPIVAWRLLFIIVGVVTIALGIAFILIIPDTPSKAWFLNEEEKLLVVERIRSNKQ
GFGNRKIKMDQVKEAFCDVRLYLNFFVMVLINIPNGGLTNFGSKYHKKKDNQITRFVL
TFYRYCADRHGIRHFGCSSDEPSTRSSRGCRTVVVRSFRC"
gene complement(<637482..>638501)
/gene="DAL5"
/locus_tag="AWJ20_4818"
/db_xref="GeneID:30036991"
mRNA complement(<637482..>638501)
/gene="DAL5"
/locus_tag="AWJ20_4818"
/product="allantoate permease"
/transcript_id="XM_018881913.1"
/db_xref="GeneID:30036991"
CDS complement(637482..638501)
/gene="DAL5"
/locus_tag="AWJ20_4818"
/inference="similar to AA sequence:KEGG_Orthology:K08192"
/note="Allantoate permease; ureidosuccinate permease; also
transports dipeptides, though with lower affinity than for
allantoate and ureidosuccinate; expression is constitutive
but sensitive to nitrogen catabolite repression;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0015124 -
allantoate transmembrane transporter activity [Evidence
IMP,ISS] [PMID 3275614]; GO_function: GO:0042936 -
dipeptide transporter activity [Evidence IMP] [PMID
16429164]; GO_function: GO:0042936 - dipeptide transporter
activity [Evidence IGI,IMP] [PMID 17693598]; GO_function:
GO:0005215 - transporter activity [Evidence IEA];
GO_process: GO:0015719 - allantoate transport [Evidence
IMP] [PMID 3275614]; GO_process: GO:0042938 - dipeptide
transport [Evidence IMP] [PMID 16429164]; GO_process:
GO:0042938 - dipeptide transport [Evidence IGI,IMP] [PMID
17693598]; GO_process: GO:0055085 - transmembrane
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA,IEA]; GO_process: GO:0042939 -
tripeptide transport [Evidence IMP] [PMID 16429164]"
/codon_start=1
/product="allantoate permease"
/protein_id="XP_018736344.1"
/db_xref="GeneID:30036991"
/translation="MTIQENETAMGPKDLGIVEKNAMVEVAEVKDLNLKTADLAMEVA
FQSYGMEIDEATNRKILRKIDLYVCSLMCIVYAVQYLDKVTNSFASIMGLRTDLNITG
SQYSWIGSVFYIAYLVFEFPASYSLQRFPIAKTVGAFVIAWGFVLCMTALPTNYGGFL
TTRIFLGMLESAVTPAFVLLTSQWYKSEEQFTRTSFWVGCNGLGSIIGSLTAYGLAKR
QEAGTLPISGWRLLFVIVGVVTIALGIIFILVIPDVPAKAWFLTEEEKLLVVERIRSN
KQGFGNRKFKMDQVKEAFTDVRLYLNFLVMVLIDIPNGGMTNFQSKFCLYFKHFKTKS
EKKKD"
gene <641504..>642994
/gene="UTP15"
/locus_tag="AWJ20_4819"
/db_xref="GeneID:30036992"
mRNA <641504..>642994
/gene="UTP15"
/locus_tag="AWJ20_4819"
/product="Utp15p"
/transcript_id="XM_018881914.1"
/db_xref="GeneID:30036992"
CDS 641504..642994
/gene="UTP15"
/locus_tag="AWJ20_4819"
/inference="similar to AA sequence:KEGG_Orthology:K14549"
/note="Nucleolar protein; component of the small subunit
(SSU) processome containing the U3 snoRNA that is involved
in processing of pre-18S rRNA; GO_component: GO:0030686 -
90S preribosome [Evidence IDA] [PMID 12150911];
GO_component: GO:0005730 - nucleolus [Evidence IEA,IEA];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
12068309]; GO_component: GO:0005634 - nucleus [Evidence
IEA]; GO_component: GO:0033553 - rDNA heterochromatin
[Evidence IDA] [PMID 15489292]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0032040 - small-subunit processome [Evidence IDA] [PMID
12068309]; GO_component: GO:0034455 - t-UTP complex
[Evidence IDA] [PMID 17515605]; GO_function: GO:0030515 -
snoRNA binding [Evidence IPI] [PMID 12068309]; GO_process:
GO:0000462 - maturation of SSU-rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 12068309]; GO_process: GO:0045943 - positive
regulation of transcription from RNA polymerase I promoter
[Evidence IMP] [PMID 15489292]; GO_process: GO:0006364 -
rRNA processing [Evidence IEA,IEA]; GO_process: GO:0042254
- ribosome biogenesis [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Utp15p"
/protein_id="XP_018736345.1"
/db_xref="GeneID:30036992"
/translation="MSTQAVRIQPVRNPVLPAKTTPELRYWRGFKNPLLVKENNAVNH
LHFNPQSPHDFAVTSSTRVQIFSAKTRQVVRTIARFKDTVYSGEFRSDGKLLVAGDAT
GLIQVFDANSRSVLVTLLPTQHSTQVTKFHPTELSTLLSASDDRVVRLWDLTSPNPVA
SYGDHTDYVRTACFIPQSSLIATGCYDGLVRIYDRRISAEEGPVATHDQGAPVESVLA
LNSTTFLSAGDPTVKVWDLAASKKVKELGNFQKTVTCLADAGERGVLAGSLDGHVKVL
DSSSPDWSVKFGWKFGGAVLNTGVSPDHKHFVTGLTSGLLSIRTRKTEPKVAQGVKTA
KVGNFARMVRGAEYHGELEHRVLDTKSAPSRKLKAYERHLKAFRWSDALDSAFSNDMA
PELTTTVLEELKRRGKVIVSLSGRDEDSLEPILKWSVKAVQDTRSIPIVADWVGCIVD
MYANLIDRSPVLENLVLQLRKRVALEVERAKEAKRIDGMLELLLSR"
gene complement(<643490..>644761)
/locus_tag="AWJ20_4820"
/db_xref="GeneID:30036994"
mRNA complement(<643490..>644761)
/locus_tag="AWJ20_4820"
/product="hypothetical protein"
/transcript_id="XM_018881916.1"
/db_xref="GeneID:30036994"
CDS complement(643490..644761)
/locus_tag="AWJ20_4820"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736346.1"
/db_xref="GeneID:30036994"
/translation="MGTGPSQSEIHKIKLEIKKWEYSFQETHGRKPGYDDIRAIPDIH
IKYKTYNRYKSGTSRASKNVDDNDQAAVRKLDKPLKSLAQYDQSTDGVTADSTGYVLT
PHGTRSYREDLSIEYISRSPSRRSAEYDEGFKGNAASHRTPSTPQKKKNAEMAAAILA
AKIGPTPQMDGRVLGIFEFTSPDAILQTPSAKKQKYNPSFENALLNTPSKKRTKSVAS
ANPISPPRSDSPVRAVDSNSHAEPDANLSHTILATPAKSKTSSPFNMETPSKTPTYLN
HRISSMADSPIAPRRLGRTLSSMMAELRDIKKEQETGGFDPDDGFDINEIADLNEEDL
YSDFNDSQDDTKPKDSTEPGPIYKKKGQKRTTKRVKMKPILNENDPIDESAKRARTLP
KKQPENFQRLKLHNSGYKTNKSKFFFGRRSR"
gene <645086..>647341
/gene="ATP25"
/locus_tag="AWJ20_4821"
/db_xref="GeneID:30036995"
mRNA <645086..>647341
/gene="ATP25"
/locus_tag="AWJ20_4821"
/product="Atp25p"
/transcript_id="XM_018881917.1"
/db_xref="GeneID:30036995"
CDS 645086..647341
/gene="ATP25"
/locus_tag="AWJ20_4821"
/note="Mitochondrial protein required for the stability of
Oli1p (Atp9p) mRNA; also required for the Oli1p ring
formation; YMR098C is not an essential gene; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence
IEA,IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IDA] [PMID 18216280]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14562095];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0048255 -
mRNA stabilization [Evidence IEA]; GO_process: GO:0048255
- mRNA stabilization [Evidence IMP] [PMID 18216280];
GO_process: GO:0033615 - mitochondrial proton-transporting
ATP synthase complex assembly [Evidence IMP] [PMID
18216280]"
/codon_start=1
/product="Atp25p"
/protein_id="XP_018736347.1"
/db_xref="GeneID:30036995"
/translation="MASRRVIYTLLEPVTRPYFAPYRHNYNQRQQEKRRQALQRKWIS
TGVSDGVVELTEEEAALVASGHATNSAGSTSGSGTSTVIVSSSSSNQAGSISSSVSGD
NEHSLLRTTSDDIPWYLRNTDTIKATESLQSREPLPDFPFASPRSLENMAKYMIEDLG
LVDLQIIDLRHVQPASPFGPNDIMLLCSGKGSRHLTKASQSLIGYVKQNYNQLADTEG
ILSSNFLRVYNRRLRKRAQRQGTANREFMAGGDSTGTMVDRTGSWVVLDTKVDGIYIH
IFTEERRSEVDLEGYWSQKSKGISELVDLPKSKFEKRFVLPGSRRGFATSIVASNVKA
TLAETVGSSSTASVAPTVKAAVADAHINNTPTAHTSKLSVDSTDSAVVEEPVPSESQN
YLDELQKWSLAGDYKRCIRLKSFAPELTAEENQKSDTLILKAHLNYLAVNQKTIIFSE
ITPTCDIVSSFINAFPTEPMPLQWRLRLIFLQKLHTINEPAFPLNLLVEHITLQQASG
VPIEPWDVEMVVATIAYSHQFTNKKVNNLSVAEWEAVCDNKLQLILQVHEVAFRSTGE
VLSVSDSLVALLYRLCFSLSKEGKAKSIVPSEALADPTPSTGVPNSYIAGVTGKRAFV
DPRATTMMGLFGGEGGAKMTKPIVILIMSVLINGGSWDKFWSFWTNINRYTIVDQDLL
AVMVSLVTKTGDQANMIHLLEKYLPTLLTDLERTGVSILPETAEALKASVQYLDPARA
RYDFIRNFLGC"
gene <648028..>648597
/locus_tag="AWJ20_4822"
/db_xref="GeneID:30036996"
mRNA <648028..>648597
/locus_tag="AWJ20_4822"
/product="hypothetical protein"
/transcript_id="XM_018881918.1"
/db_xref="GeneID:30036996"
CDS 648028..648597
/locus_tag="AWJ20_4822"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736348.1"
/db_xref="GeneID:30036996"
/translation="MDQYNKQAADFVFRANNADSGPDEIDLHGLYVKEAIQALDTRIR
AGIQRHESHIKAIVGKGNHSANHVAKIKPAVEELCQTHGIRHEIDPHNSGVIILHLDG
QSGPAVNTGNYPQQPPASHGGSASQYYGGNAPQQQPYAPQHQQYQQNYGQQQQQYQQQ
PQGQGQNNQNDMLFKIFCGVAKMLFRQCA"
gene complement(<648892..>649743)
/gene="KTI12"
/locus_tag="AWJ20_4823"
/db_xref="GeneID:30036997"
mRNA complement(<648892..>649743)
/gene="KTI12"
/locus_tag="AWJ20_4823"
/product="Kti12p"
/transcript_id="XM_018881919.1"
/db_xref="GeneID:30036997"
CDS complement(648892..649743)
/gene="KTI12"
/locus_tag="AWJ20_4823"
/inference="similar to AA sequence:KEGG_Orthology:K15456"
/note="Protein that plays a role in modification of tRNA
wobble nucleosides; protein plays role in tRNA wobble
nucleoside modification with Elongator complex; involved
in sensitivity to G1 arrest induced by zymocin; interacts
with chromatin throughout the genome; also interacts with
Cdc19p; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 12139626]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 12139626]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0003682 -
chromatin binding [Evidence IDA] [PMID 15772087];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_process: GO:0016049 - cell growth [Evidence IPI]
[PMID 12139626]; GO_process: GO:0006357 - regulation of
transcription from RNA polymerase II promoter [Evidence
IGI] [PMID 15772087]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0002098 - tRNA wobble uridine modification
[Evidence IMP] [PMID 18755837]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Kti12p"
/protein_id="XP_018736349.1"
/db_xref="GeneID:30036997"
/translation="MPLITVSGLPSSGKTTRALQLKAGLERKIAEVNSDLTVYLINDE
SLSISKETYRELHSEKATRGAQISAVKRHLGKNTIVIFDNLSYIKGFRYQLFCEAKAL
STNACLVHVGAPKDMCVAWNKSRPEGEAWPEDLFDALVFRYEEPNPMSRWDSPLFPIA
HEDTEADIPIDEIWDTLVLRKPKPPNAATVLKPVTAGNYLTELDKITTSVVNEIIDLH
KTSPGGLVKLDAYDPVVQLPMRLTVASLNRIRRNFVTINKMKPIEVSRIRPHFIEFAI
KNLSADM"
gene <650295..>651395
/gene="MTG1"
/locus_tag="AWJ20_4824"
/db_xref="GeneID:30036998"
mRNA <650295..>651395
/gene="MTG1"
/locus_tag="AWJ20_4824"
/product="putative GTPase MTG1"
/transcript_id="XM_018881920.1"
/db_xref="GeneID:30036998"
CDS 650295..651395
/gene="MTG1"
/locus_tag="AWJ20_4824"
/inference="similar to AA sequence:KEGG_Orthology:K06948"
/note="Putative GTPase peripheral to the mitochondrial
inner membrane; essential for respiratory competence,
likely functions in assembly of the large ribosomal
subunit, has homologs in plants and animals; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence
IEA,IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IDA] [PMID 12808030]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 16823961];
GO_function: GO:0005525 - GTP binding [Evidence IEA,IEA];
GO_function: GO:0003924 - GTPase activity [Evidence ISA]
[PMID 12808030]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IGI,IMP] [PMID
12808030]"
/codon_start=1
/product="putative GTPase MTG1"
/protein_id="XP_018736350.1"
/db_xref="GeneID:30036998"
/translation="MSTEVPSEPTKHKFVPRPSFPDYNIPLANFHGHHRKALQSMHQT
ALQVDLVLELRDSRAPLSSINLLFDKVLGHKNKLVLYTKNDLSGIKNATELYEAWHPH
QKFLNIDCRSTADANKVLSAAKQLYDELEPKPPLGLRLLITGMPNAGKSTFLNTLRKV
GTGERHKVASTGEMPGVTRSISETIRISRDPNIYIFDTPGVFVPQVKNSEDMLKMCLI
NAVKRSHVDPVVLADYLLYRINKQYPDGKPYLKYTGRPTNNIDFLLRSIVKRRLSRRN
SATKDNNKAKASNNGDKSKGTKIVDFNENGEALDWIEKWTKGKGPKIVLDDVNEPDCY
AKTIEEQQTRLSSFDLGPLKVKSFQKKRLMLR"
gene <654564..>654869
/gene="POM33"
/locus_tag="AWJ20_4825"
/db_xref="GeneID:30036999"
mRNA <654564..>654869
/gene="POM33"
/locus_tag="AWJ20_4825"
/product="nucleoporin POM33"
/transcript_id="XM_018881921.1"
/db_xref="GeneID:30036999"
CDS 654564..654869
/gene="POM33"
/locus_tag="AWJ20_4825"
/codon_start=1
/product="nucleoporin POM33"
/protein_id="XP_018736351.1"
/db_xref="GeneID:30036999"
/translation="MAYAANFEFGLVFLLFFKFVTFRKGTTFPFLFYIVFIKARYDQS
YYMKSAVKAWEVKIDSVFANPNIPAPLKQAWAQVKDVVKRITSAVTIGKPVAPKKHS"
gene <655610..>656128
/gene="ADI1"
/locus_tag="AWJ20_4826"
/db_xref="GeneID:30037000"
mRNA <655610..>656128
/gene="ADI1"
/locus_tag="AWJ20_4826"
/product="acireductone dioxygenase (Ni2+-requiring)"
/transcript_id="XM_018881922.1"
/db_xref="GeneID:30037000"
CDS 655610..656128
/gene="ADI1"
/locus_tag="AWJ20_4826"
/inference="similar to AA sequence:KEGG_Orthology:K08967"
/note="Acireductone dioxygenease involved in the
methionine salvage pathway; ortholog of human MTCBP-1;
transcribed as polycistronic mRNA with YMR010W and
regulated post-transcriptionally by RNase III (Rnt1p)
cleavage; ADI1 mRNA is induced in heat shock conditions;
GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14562095]; GO_function:
GO:0010308 - acireductone dioxygenase (Ni2+-requiring)
activity [Evidence IMP] [PMID 15938715]; GO_function:
GO:0010309 - acireductone dioxygenase [iron(II)-requiring]
activity [Evidence IEA,IEA,IEA]; GO_function: GO:0051213 -
dioxygenase activity [Evidence IEA]; GO_function:
GO:0005506 - iron ion binding [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_process: GO:0019509 - L-methionine
salvage from methylthioadenosine [Evidence IEA,IEA,IEA];
GO_process: GO:0019509 - L-methionine salvage from
methylthioadenosine [Evidence IGI,IMP,ISS] [PMID
15938715]; GO_process: GO:0019509 - L-methionine salvage
from methylthioadenosine [Evidence IMP] [PMID 18625006];
GO_process: GO:0008652 - cellular amino acid biosynthetic
process [Evidence IEA]; GO_process: GO:0009086 -
methionine biosynthetic process [Evidence IEA];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]"
/codon_start=1
/product="acireductone dioxygenase (Ni2+-requiring)"
/protein_id="XP_018736352.1"
/db_xref="GeneID:30037000"
/translation="MKIYYHDGLEGDQRLRHDSGKEVTVEELDKLAVKAYHFDDIKDV
DKLAESRNYINRDVINVSPEALGGKESYETKIKTFYTEHLHEDEEIRYVLDGEGYFDV
RDKSDRWIRCLLTKGDLLILPAGIYHRFTTASNDYIQAMRLFQAEPKWIPHNRPCDNN
EYRVEYVKSISV"
gene <663078..>665963
/gene="VPS53"
/locus_tag="AWJ20_4827"
/db_xref="GeneID:30037001"
mRNA <663078..>665963
/gene="VPS53"
/locus_tag="AWJ20_4827"
/product="Vps53p"
/transcript_id="XM_018881923.1"
/db_xref="GeneID:30037001"
CDS 663078..665963
/gene="VPS53"
/locus_tag="AWJ20_4827"
/note="Component of the GARP (Golgi-associated retrograde
protein) complex; GARP is required for the recycling of
proteins from endosomes to the late Golgi, and for mitosis
after DNA damage induced checkpoint arrest; required for
vacuolar protein sorting; members of the GARP complex are
Vps51p-Vps52p-Vps53p-Vps54p; GO_component: GO:0000938 -
GARP complex [Evidence IPI] [PMID 10637310]; GO_component:
GO:0005794 - Golgi apparatus [Evidence IEA,IEA];
GO_component: GO:0005794 - Golgi apparatus [Evidence IDA]
[PMID 10637310]; GO_component: GO:0005794 - Golgi
apparatus [Evidence TAS] [PMID 11689439]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005768 - endosome [Evidence IEA];
GO_component: GO:0010008 - endosome membrane [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0006896 - Golgi to vacuole transport
[Evidence IMP] [PMID 10637310]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0042147 -
retrograde transport, endosome to Golgi [Evidence IDA]
[PMID 12686613]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Vps53p"
/protein_id="XP_018736353.1"
/db_xref="GeneID:30037001"
/translation="MASFGVANGHSGGSGLGGEDDDPLSLPNYSPVDHLNVLFPTSAN
VQRVGDIRDLAQLYGQSLESKLINGRRTSGSTGSSSIEAVSKTLEEVRSLTDQIDSLR
TQAASTDQAITAMTLDIKKLDNTKANLIQSITVLKRLQMLTTAYNQLSSLVARRQYKE
MSQTLPAVMELMAHFRQFRSISQIATLSKQVSDIQVRISDQIFDDFATVIEHKPGSRT
VAGSAGVSGTGTPVLNGSSTGFSGSGAGSGTGSATQTGAPASIVDISATLADACVVLD
SLPGSTARDRLISWFCNVQLKEYRTIFRSSDEAGSLENISRRYSYLKRLLKTHSDEYA
RYFVPSWNVTEELCKRFCATTREDIQGLLSQVSSTQTNPKESDVQLLLNSLNETLEFE
QYLEKKFKYMNPRPASSSSTSSSEPFKFANSVSIAFQPHLNIWIQFQDRALAQKFQEF
RAVSRHSQSQGTRSQTGSDDNDQENKDDDDPTVLPSSADLFIFYRSVLTQTAKLSNRE
PLLQLSNLFGKWLTVYCSQILRSYIPDRLVTTDDIKTICLVITTADYCFNTTNQLEQK
LVDQLDPEFKVGVDLEREKGMFLEVINLCIRNLVRKVEAGCEFAWREMANTNWSKVET
VGDQSSYVADLTSNIERDTLAILECMKKETHARMLCDKIVEAVTGDFLQSVVRCRPIS
EVSAEQMLLDLYVLKGCFLKLPTLAQKQHDHGSMDINGTDKSSTGIHNNDSGTTNGLT
TGSETSTVSSAYTRHVTKSLSRVETILKVILTQVNPAEGLVQNYFYLVGDKSQQNFAK
ILELKGITRGGQTRFLEMFNAHLKAHDNLVDESPILSSLQLNPLVGKGTVVGGSRVSS
ASHINIGIGGLGGAAGIMGSGSGINLVGSNNSSNNNSGSSSGNPFQNGSHLFDTKGPS
ILSKEGFERFTHGAEAPVNKLNENFRNFGRLFRRDGSGSPLGNRNNH"
gene complement(<666271..>667101)
/gene="PML1"
/locus_tag="AWJ20_4828"
/db_xref="GeneID:30037002"
mRNA complement(<666271..>667101)
/gene="PML1"
/locus_tag="AWJ20_4828"
/product="Pml1p"
/transcript_id="XM_018881924.1"
/db_xref="GeneID:30037002"
CDS complement(666271..667101)
/gene="PML1"
/locus_tag="AWJ20_4828"
/inference="similar to AA sequence:KEGG_Orthology:K13108"
/note="Subunit of the RES complex; RES complex is required
for nuclear retention of unspliced pre-mRNAs; acts in the
same pathway as Pml39p and Mlp1p; GO_component: GO:0070274
- RES complex [Evidence IDA] [PMID 15565172];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008380 -
RNA splicing [Evidence IEA]; GO_process: GO:0006406 - mRNA
export from nucleus [Evidence IMP] [PMID 15565172];
GO_process: GO:0006397 - mRNA processing [Evidence IEA];
GO_process: GO:0000398 - mRNA splicing, via spliceosome
[Evidence IDA] [PMID 15565172]; GO_process: GO:0051237 -
maintenance of RNA location [Evidence IMP] [PMID
16162818]"
/codon_start=1
/product="Pml1p"
/protein_id="XP_018736354.1"
/db_xref="GeneID:30037002"
/translation="MSDGNEGRDRYGRVKREYRDEDWHERQRRGPRNQNRSRSPGRRP
GSGARTGANAVPVKREDRGDGGRTRSHERNRDRDRDRDMRREWDRGSRDRSGIRPKEE
DLWSNDDSKEAPPSPPPVKPNYSHSGVLTEESKKDSEGNILKYHEPEDATTPPKTPEY
RIYVFSESEQGKLIDTIKLNQKSHYLLGRDTKVCDIPVDIKSCSRQHAVIQFRRISKT
DRFGDTHHTIKPYIIDLESSYGTQLNGDDVPPSRYVELRNKDLIQFCGHPREYLWIES
"
gene <667803..>670199
/gene="HCA4"
/locus_tag="AWJ20_4829"
/db_xref="GeneID:30037003"
mRNA <667803..>670199
/gene="HCA4"
/locus_tag="AWJ20_4829"
/product="RNA-dependent ATPase HCA4"
/transcript_id="XM_018881925.1"
/db_xref="GeneID:30037003"
CDS 667803..670199
/gene="HCA4"
/locus_tag="AWJ20_4829"
/inference="similar to AA sequence:KEGG_Orthology:K14776"
/note="DEAD box RNA helicase; high-copy number suppression
of a U14 snoRNA processing mutant suggests an involvement
in 18S rRNA synthesis; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IC] [PMID 16209945]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0004004 - ATP-dependent RNA helicase activity [Evidence
IMP] [PMID 16209945]; GO_function: GO:0004004 -
ATP-dependent RNA helicase activity [Evidence IDA] [PMID
23153376]; GO_function: GO:0008026 - ATP-dependent
helicase activity [Evidence IEA]; GO_function: GO:0003723
- RNA binding [Evidence IEA]; GO_function: GO:0008186 -
RNA-dependent ATPase activity [Evidence IDA] [PMID
18975973]; GO_function: GO:0008186 - RNA-dependent ATPase
activity [Evidence IDA] [PMID 23153376]; GO_function:
GO:0004386 - helicase activity [Evidence IEA,IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0003676 - nucleic acid binding
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006200 - ATP
catabolic process [Evidence IEA]; GO_process: GO:0006364 -
rRNA processing [Evidence IEA]; GO_process: GO:0006364 -
rRNA processing [Evidence IMP] [PMID 16209945];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA]"
/codon_start=1
/product="RNA-dependent ATPase HCA4"
/protein_id="XP_018736355.1"
/db_xref="GeneID:30037003"
/translation="MSSNGHGKRDTNGSKHSVNGKKNAHNKNGGGANNNKSKHVKRQS
ENAHIAALETQIKEWDPAEHPNLTKFEQLPLSRATLQGLRSSHFVDMTDIQRQSLMAS
LKGEDILGAARTGSGKTLAFLIPVLERLYRQKWNQQDGLGALIISPTRELAIQIFQVL
RKIGRAHSLSAGLVIGGKDVQVESERLARLNILIGTPGRLLQHLDQTAGFDVSNLQML
VLDEADRILDMGFKKSLDAIIDNLPTTRQTLLFSATQTKTVSDLARLSLSDPRYISVH
EEDQTATPKGLEQFYIVSPLDEKLDNMYAFIKSHIKTKMLIFFSSSKQVRFAYESFRK
LQPGIPLLHLHGKQKQQARIDVTQKFSSAQYSCLFATDIVARGIDFPAVDWVIQVDAP
EDAATYIHRVGRSARFDKKGRALLFVTPSEEKGMISALESRKVPISKLTIREAKKKSI
RQDLQALCFQDPELKYLGQKAFISYVRSVYIQKNKDIFNFESLPLEKFAESLGLPGAP
NIKIKGGERSKEAKNAPRQLLKLTKEPGSDDEQEGEDKKKEPEKVRTKYDRMFERKNQ
NVLSEHYLNLTKTAGDEDDGDDFIAVKRQDHIIDDEEIPNLLEIPTSKRAQKRALSKK
LSTKSKGNATKLVFDDEGNAHAIYELEDEEDFKKAGDAAVQKAEFVARESAAMQEKDI
EDKQVARDKRVEKKRRRQEIERRALEAEQDESDDGEGGYQVTLGGADDESDYDHNQEP
DIDRDYESSDNDEPAAKKPKWFQKDKDSAPVRNGVLEVEEPDTLEDLEALSARLIRGR
"
gene <671459..>672370
/gene="TPP1"
/locus_tag="AWJ20_4830"
/db_xref="GeneID:30037005"
mRNA <671459..>672370
/gene="TPP1"
/locus_tag="AWJ20_4830"
/product="Tpp1p"
/transcript_id="XM_018881927.1"
/db_xref="GeneID:30037005"
CDS 671459..672370
/gene="TPP1"
/locus_tag="AWJ20_4830"
/inference="similar to AA sequence:KEGG_Orthology:K08073"
/note="DNA 3'-phosphatase; functions in repair of
endogenous damage of double-stranded DNA, activity is
specific for removal of 3' phosphates at strand breaks;
similar to the l-2-haloacid dehalogenase superfamily;
homolog of human polynucleotide kinase/3'-phosphatase;
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IC] [PMID
11278831]; GO_component: GO:0005634 - nucleus [Evidence
IC] [PMID 15222769]; GO_function: GO:0003690 -
double-stranded DNA binding [Evidence IDA] [PMID
15222769]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0046403 - polynucleotide
3'-phosphatase activity [Evidence IEA]; GO_function:
GO:0046403 - polynucleotide 3'-phosphatase activity
[Evidence IDA] [PMID 11278831]; GO_function: GO:0046403 -
polynucleotide 3'-phosphatase activity [Evidence IMP]
[PMID 15222769]; GO_process: GO:0006281 - DNA repair
[Evidence IEA]; GO_process: GO:0006974 - cellular response
to DNA damage stimulus [Evidence IEA]; GO_process:
GO:0006302 - double-strand break repair [Evidence IGI,IMP]
[PMID 11585902]; GO_process: GO:0098506 - polynucleotide
3' dephosphorylation [Evidence IEA]"
/codon_start=1
/product="Tpp1p"
/protein_id="XP_018736356.1"
/db_xref="GeneID:30037005"
/translation="MGPKRKAVGTAASFFQSKKLAVAKPTSIVSTSGNDERNVDEDVV
LMPGLRWTLNGTLVRAVYTPTNTEEQKGSVPEEEREQTSIPPSHTESETKNAGASSPK
RLAIAAFDLDSTLVTTKGRSPFPRDGSDWKWLDTSVRDKLQKLVNRTVVKNGKSENEE
FSVNQGADVSTQDASMVDPSTEYVLAIISNQGGVVAENDKKRYLYLKDRVKSIVKDLD
TVPLRFYAATKPKKGASDKYRKPLTGMWEALQADLAPVYSVDIEKSFFVGDAAGRRSD
FSDSDLKFAQAVGLRFFTPEKFFGLSD"
gene complement(<672801..>673370)
/gene="RPL9B"
/locus_tag="AWJ20_4831"
/db_xref="GeneID:30037006"
mRNA complement(<672801..>673370)
/gene="RPL9B"
/locus_tag="AWJ20_4831"
/product="ribosomal 60S subunit protein L9B"
/transcript_id="XM_018881928.1"
/db_xref="GeneID:30037006"
CDS complement(672801..673370)
/gene="RPL9B"
/locus_tag="AWJ20_4831"
/inference="similar to AA sequence:KEGG_Orthology:K02940"
/note="Ribosomal 60S subunit protein L9B; homologous to
mammalian ribosomal protein L9 and bacterial L6; RPL9B has
a paralog, RPL9A, that arose from a single-locus
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0022625 - cytosolic
large ribosomal subunit [Evidence IDA] [PMID 18782943];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IEA,IEA]; GO_function: GO:0019843 - rRNA binding
[Evidence IEA]; GO_function: GO:0003735 - structural
constituent of ribosome [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IDA] [PMID 18782943]; GO_process: GO:0002181 - cytoplasmic
translation [Evidence IDA] [PMID 18782943]; GO_process:
GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="ribosomal 60S subunit protein L9B"
/protein_id="XP_018736357.1"
/db_xref="GeneID:30037006"
/translation="MKFIQTDQVLEVPEGVTVDIKSRIVTVTGPRGTLVKNLRHADVT
FTKVNDKQIRLTVHHGDRKHVATLRTVKSLVANLITGVTKGYKYKMRYVYAHFPINVN
LLEDGKVVEIRNFLGEKRVRTIHVLEGVTVSISSAQKDELILEGNSLENVSQSAADIQ
QICRVRNKDIRKFLDGIYVSERGLIEEDA"
gene <673083..>673424
/locus_tag="AWJ20_4832"
/db_xref="GeneID:30037007"
mRNA <673083..>673424
/locus_tag="AWJ20_4832"
/product="hypothetical protein"
/transcript_id="XM_018881929.1"
/db_xref="GeneID:30037007"
CDS 673083..673424
/locus_tag="AWJ20_4832"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736358.1"
/db_xref="GeneID:30037007"
/translation="MGIDVSHLVLVTLGDTSDQVGDEGLNSTEGSNVLSVTVVDSKTD
LLVVDLGEGNISVTEVLDKSTTGTSNGDDSGLDVDSDALRDFKDLIGLNELHFCAVMG
TMSILRRSLNA"
gene <674426..>675199
/locus_tag="AWJ20_4833"
/db_xref="GeneID:30037008"
mRNA <674426..>675199
/locus_tag="AWJ20_4833"
/product="hypothetical protein"
/transcript_id="XM_018881930.1"
/db_xref="GeneID:30037008"
CDS 674426..675199
/locus_tag="AWJ20_4833"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736359.1"
/db_xref="GeneID:30037008"
/translation="MLSRSVLAQARTIGSSRIAGRRTGAMASTILQRGLKFQSKQYNP
SPGFSVFSEELEQEIRHKLTSLPSSNKVFQNADGTARNPSDEELKVVAELGVLAQGDR
ATFLDRFRLSQEQRELLAENSYLLDDYVNNGKRILDKIPVEDPTTGEITWTVIRENQK
EGWENLIYYGYVPALLISLFFALFLDKENISDWAMEELRLRAQERYNSGMEEINNSEL
SAEERKKQDALIVERIISGDYDRLAGLRKAGSDLPASLL"
gene complement(<675471..>677702)
/locus_tag="AWJ20_4834"
/db_xref="GeneID:30037009"
mRNA complement(<675471..>677702)
/locus_tag="AWJ20_4834"
/product="hypothetical protein"
/transcript_id="XM_018881931.1"
/db_xref="GeneID:30037009"
CDS complement(675471..677702)
/locus_tag="AWJ20_4834"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736360.1"
/db_xref="GeneID:30037009"
/translation="MDRQAGFLGKNERGEGTRPEIEHNTVNASSNVHKASPRNPLDSF
DNDISGGPFASSSNYSTTAHPNTTISPQTPPASELETLPTPLPSGDVYHDKGEHSRVT
NIDMSASEEASRSATVNSGSVEIKELTEDEDIDDESEEEIGEDDEEYVDIDSSPTKRK
SSQRRRSRGSHKVNDGSRPSSPTPSGGRNAVAPKPEPDSGNPLSGLGQSLPRSGPSVP
QALPMNWRNPPPEPAGASEFVSLTEQDIETLPESSLRPTLQRATKTIQEYRAALRKYE
SLIAHYKLQNKLLTIETHEAAQRHEVETSIAKREVDRLRIETMAAAKAAQNAAAAKAA
APSSEIIDDLQSALESYKRRLRKTRTKLQIATDQLEDKDEEIERLRRGLSDMATSFSR
ESGPSVTVPTSGPPSHGPVVGPLPGMSSGPPINSPVSMAPGGPVYYANTPGPSQQPPI
GPMSPSQNLRTPFTHLPPRQHQRDSSSASDDSGQGLSALEMLASQVLSQQRIPPSPLR
RQPQQHENIQQQQLQQQQQQSQQPLLPLPTPSRQRSQGLMSPLEFNEASSVPSLHAVQ
AMSPEKRRRESSASTITIPTDGEDEGDEHDVNGTDEEVEDDDDDDYKESAGRDESTAV
RRSGRLSREGKRRSGGSAQVFTTGAETDVEESDDSKDDSDIDNGRQGGMRSSRGTRSQ
RQNSRQRVPVSSESPNRRSSPGSPKKYIRKERNDTQSSSKPSASSSSSKRMPVGRILD
FGN"
gene <682334..>683296
/gene="GET3"
/locus_tag="AWJ20_4835"
/db_xref="GeneID:30037010"
mRNA <682334..>683296
/gene="GET3"
/locus_tag="AWJ20_4835"
/product="guanine nucleotide exchange factor GET3"
/transcript_id="XM_018881932.1"
/db_xref="GeneID:30037010"
CDS 682334..683296
/gene="GET3"
/locus_tag="AWJ20_4835"
/inference="similar to AA sequence:KEGG_Orthology:K01551"
/note="Guanine nucleotide exchange factor for Gpa1p;
amplifies G protein signaling; subunit of the GET complex,
which is involved in Golgi to ER trafficking and insertion
of proteins into the ER membrane; has low-level ATPase
activity; protein abundance increases in response to DNA
replication stress; GO_component: GO:0043529 - GET complex
[Evidence IEA]; GO_component: GO:0043529 - GET complex
[Evidence IPI] [PMID 16269340]; GO_component: GO:0005794 -
Golgi apparatus [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IEA,IEA];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 14562095]; GO_function: GO:0005524 - ATP
binding [Evidence IEA,IEA,IEA]; GO_function: GO:0016887 -
ATPase activity [Evidence IEA]; GO_function: GO:0016887 -
ATPase activity [Evidence IDA] [PMID 12680698];
GO_function: GO:0005085 - guanyl-nucleotide exchange
factor activity [Evidence IDA] [PMID 18261907];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006200 - ATP
catabolic process [Evidence IEA]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0000750 -
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [Evidence IMP] [PMID
18261907]; GO_process: GO:0006620 - posttranslational
protein targeting to membrane [Evidence IDA] [PMID
20850366]; GO_process: GO:0045048 - protein insertion into
ER membrane [Evidence IEA]; GO_process: GO:0045048 -
protein insertion into ER membrane [Evidence IMP] [PMID
18724936]; GO_process: GO:0046685 - response to
arsenic-containing substance [Evidence IEA]; GO_process:
GO:0009408 - response to heat [Evidence IMP] [PMID
12680698]; GO_process: GO:0010038 - response to metal ion
[Evidence IMP] [PMID 12680698]; GO_process: GO:0006890 -
retrograde vesicle-mediated transport, Golgi to ER
[Evidence IDA,IGI,IMP] [PMID 16269340]; GO_process:
GO:0006810 - transport [Evidence IEA]; GO_process:
GO:0016192 - vesicle-mediated transport [Evidence IEA]"
/codon_start=1
/product="guanine nucleotide exchange factor GET3"
/protein_id="XP_018736361.1"
/db_xref="GeneID:30037010"
/translation="MSEELNLEPSLQNIIDQKSLRWIFVGGKGGVGKTTTSCSLALQL
AKNRENVLLISTDPAHNLSDAFGQKFGKDARPVNGVPNLHAMEIDPNGSIQEFLDQPQ
NQAASSMMQDIAFSIPGVDEALSFAEVLKQVKAQSYSVIVFDTAPTGHTLRFLQFPTI
LEKALGKISEISGRFGPMLNSLMGASGTNTEDMLAKLESTRETIAEVNKQFQNPDLTT
FVCVCIAEFLSLYETERMVQELTSYGIDTHCIVVNQLLFPKTTSECEQCNARYKMQQK
YLEQILELYEDFHIVQLPQLTSEVRGLESLQTFSEKLIHPYVPN"
gene complement(<683565..>685508)
/locus_tag="AWJ20_4836"
/db_xref="GeneID:30037011"
mRNA complement(<683565..>685508)
/locus_tag="AWJ20_4836"
/product="hypothetical protein"
/transcript_id="XM_018881933.1"
/db_xref="GeneID:30037011"
CDS complement(683565..685508)
/locus_tag="AWJ20_4836"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736362.1"
/db_xref="GeneID:30037011"
/translation="MVNGTTKISNETVELSSLPSVKQKGEVWLTLGGPKDGDDVKLLL
RWVPMVITFSTGETGDGHINVREELIKDLEPFDIKLSSELHQLTKFYMKPTSNSSKLL
TALVKCIPIVTFEFSKALKKACEVLSGDDSMETDFYKNFPNAEQYIPDPAMAPNSDRM
KLFENKVFIFPQTKERDALLGAITSGGGKAYVYEMTSAHEPPSRIKDFVLSKVKNIDN
LVLVGYHTSERSNQEDIDKLLVLNNAAKMIGTRLFAASDFLTVILEADASKLNLRRPD
EDGAPVSFTEQPKRLGTASFLETSIRVEAERKPGPIRRRKPQTKQLNIMDLMATTKRE
PDMPVLAPNQADSLSMNKVADSMAIDAKDGAVETADSQATAKEPDTHGETRSPSRRKR
RERINDDIITQTSTFPPDVTQNAREYKRRKLDLQQASDNKSSNVDDDMFAESVPALIE
DEDNNPDEDSSEPKDGHVGVIKKWSGDLDDDSEDSDDDLAGFENLAIVEATLEFRQHL
PKPKAPTVKSKRPNFKGFRMKEPRKPPSMKVDYMEAAPTDMLLEQDMSMFANKERYTK
RKLPPVESLQNSSRSKRRKETKTQAAENMFVNSDDSLSEDESEVGMNNYSSAISNESR
PVHSMDDYDEDDEDELSFRFSKN"
gene complement(<686796..>688427)
/gene="VPS35"
/locus_tag="AWJ20_4837"
/db_xref="GeneID:30037012"
mRNA complement(<686796..>688427)
/gene="VPS35"
/locus_tag="AWJ20_4837"
/product="Vps35p"
/transcript_id="XM_018881934.1"
/db_xref="GeneID:30037012"
CDS complement(686796..688427)
/gene="VPS35"
/locus_tag="AWJ20_4837"
/inference="similar to AA sequence:KEGG_Orthology:K18468"
/note="Endosomal subunit of membrane-associated retromer
complex; required for retrograde transport; receptor that
recognizes retrieval signals on cargo proteins, forms
subcomplex with Vps26p and Vps29p that selects cargo
proteins for retrieval; interacts with Ypt7p;
GO_component: GO:0005768 - endosome [Evidence IPI] [PMID
9700157]; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 22593205]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_component:
GO:0030904 - retromer complex [Evidence IEA];
GO_component: GO:0030904 - retromer complex [Evidence
IMP,IPI] [PMID 9700157]; GO_component: GO:0030906 -
retromer complex, inner shell [Evidence IPI] [PMID
9700157]; GO_function: GO:0008565 - protein transporter
activity [Evidence IMP,IPI] [PMID 9700157]; GO_process:
GO:0045053 - protein retention in Golgi apparatus
[Evidence IMP] [PMID 8649377]; GO_process: GO:0015031 -
protein transport [Evidence IEA,IEA]; GO_process:
GO:0042147 - retrograde transport, endosome to Golgi
[Evidence IEA]; GO_process: GO:0042147 - retrograde
transport, endosome to Golgi [Evidence IPI] [PMID
9700157]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Vps35p"
/protein_id="XP_018736363.1"
/db_xref="GeneID:30037012"
/translation="MKELEQDDDGDVTKKLGDVTIGDSQESKPVGDNQTESETGDDKP
KATSEDESEGKDNGSGENLFEKFWSHFNSLVQAHPDLPLPDKTALLLGIAKLSLNWYP
DRKDYIDRILQYVATIQQQLHADKEAAVNVQELLLSLINFYPDFLTVLSIPGYQPLLS
SQPISTQQAIAGSVINAVLKQSKKITELDDVKGVFELVSIVIKDGTASAGHSTEFPPG
GTSTKKQTASTPDISNDSEEIVADQSKLAQLVHLVYNKNPDVHAKLLAAAKKALSDGG
MRIKYTYPAVVTSAFKLVRRYQRRKSDSEKAAKIGSSFKFIHRLIDELYNSGRSELAL
RLYVNVASLADQVNAEEASYEFFAEAFTVYEESVSDSRAQFQALCIIAGALQSTRNFT
ADNYDTLVSKCALHASKLLKKPDQCRAVYLASHLWWAVEIPGRTEEELFRDDKRVLEC
LQRSLRVADACMDVAVSVELFVEILNRYIYYFDHGNSAVTVRYINGLINLIQTNLNNN
ADEKISDSPRKHFERTLEYITQQKSVDDKFQEIVW"
gene complement(<688514..>689552)
/gene="VPS35"
/locus_tag="AWJ20_4838"
/db_xref="GeneID:30037013"
mRNA complement(join(<688514..688525,688614..>689552))
/gene="VPS35"
/locus_tag="AWJ20_4838"
/product="Vps35p"
/transcript_id="XM_018881935.1"
/db_xref="GeneID:30037013"
CDS complement(join(688514..688525,688614..689552))
/gene="VPS35"
/locus_tag="AWJ20_4838"
/inference="similar to AA sequence:KEGG_Orthology:K18468"
/note="Endosomal subunit of membrane-associated retromer
complex; required for retrograde transport; receptor that
recognizes retrieval signals on cargo proteins, forms
subcomplex with Vps26p and Vps29p that selects cargo
proteins for retrieval; interacts with Ypt7p;
GO_component: GO:0005768 - endosome [Evidence IPI] [PMID
9700157]; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 22593205]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_component:
GO:0030904 - retromer complex [Evidence IEA];
GO_component: GO:0030904 - retromer complex [Evidence
IMP,IPI] [PMID 9700157]; GO_component: GO:0030906 -
retromer complex, inner shell [Evidence IPI] [PMID
9700157]; GO_function: GO:0008565 - protein transporter
activity [Evidence IMP,IPI] [PMID 9700157]; GO_process:
GO:0045053 - protein retention in Golgi apparatus
[Evidence IMP] [PMID 8649377]; GO_process: GO:0015031 -
protein transport [Evidence IEA,IEA]; GO_process:
GO:0042147 - retrograde transport, endosome to Golgi
[Evidence IEA]; GO_process: GO:0042147 - retrograde
transport, endosome to Golgi [Evidence IPI] [PMID
9700157]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Vps35p"
/protein_id="XP_018736364.1"
/db_xref="GeneID:30037013"
/translation="MSSNPPAPLSPEEQARHLEESLQFIRQQEHQMRKCLETKGKLLD
AIRHASTFLAELRSSVLTPKQYYELYIAVFDALRHLGDFLREDHPNHHLADIYELVQY
AGNIVPRLYLMITVGTAYMSVPDAPVREIMKDMMEMCRGVQHPIRGLFLRYYLSQGTR
DYLPSGEDVNGTNGGVITAAVATTSSDDSAAGPGETKNVADTRSNTSNGNGSETTITG
PGSASAVTSAPVIDPELRGNLKDSIQFIITNFIEMNKLWVRLQHQGHSREREKRTKER
QELQILVGSNLVRLSQLEGIDKEYYKESILPAILEQVTST"
gene complement(<692048..>694123)
/locus_tag="AWJ20_4839"
/db_xref="GeneID:30037014"
mRNA complement(<692048..>694123)
/locus_tag="AWJ20_4839"
/product="hypothetical protein"
/transcript_id="XM_018881936.1"
/db_xref="GeneID:30037014"
CDS complement(692048..694123)
/locus_tag="AWJ20_4839"
/inference="similar to AA sequence:KEGG_Orthology:K03351"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736365.1"
/db_xref="GeneID:30037014"
/translation="MDILVFAPESGGIALYRISGQRIWSIPGKRGAGHVIRSSWRDDG
KVLAVLYSDGSYQVIRTSNGKPVLSSGSGDSAIDLSWAEGDITANSGDGSKSEWSSSF
KLMETDVLDSLPRLQALPTKPGLLIDVKSSENSISNFDDALSVVLTGDKSGSMNLSMF
GIFSIDGIPISNSNIKARDQSQHEPSAHFLKINSNPKDISRGTIIVQNDESKAFVYHK
FHLGFIQKFGQNYLTEVSVIPAQISILIEYLQESVALLRKEANTIVEANNKFISLESL
NHMRGYLFDFLITGTMCKKITKWIESNNEMGYKRWNKTTITSYDVSNKILYENILPAI
ERLVVLLSRLRGLAKWKERGVPLGLSGEELDKTIRYAECFFKLTNKCLWEFTDEYEKF
RSFALWLELGFESVLDRVVGFSRADEPATVRTTDIMKYITELLPGSVLTSYLTTSTVG
VGDEKIDLKQKSDTVNSDSQPLEDHDVSVSQGATSKSTGDASLDSIENLSLANCLENI
DSTFSATTTKIESAMKKLISVDVGIPLASSDFNVRQAYFRGTSFVLIYGQDAFLYMVR
IHDSGIEVAKIKCENTIIQAEFINESEIILLVVDSDSRYHLQVANYESVAYTALDGKS
DLLNQTVATLTHNLDSVDLAPSNTHSFDKDFTPGSFVVNGRSGHELACVLNAERQRYA
LIYLHVEQA"
gene <694829..>696511
/gene="TMA64"
/locus_tag="AWJ20_4840"
/db_xref="GeneID:30037016"
mRNA <694829..>696511
/gene="TMA64"
/locus_tag="AWJ20_4840"
/product="Tma64p"
/transcript_id="XM_018881938.1"
/db_xref="GeneID:30037016"
CDS 694829..696511
/gene="TMA64"
/locus_tag="AWJ20_4840"
/inference="similar to AA sequence:KEGG_Orthology:K15027"
/note="hypothetical protein that associates with
ribosomes; has a putative RNA binding domain; in mammals
the corresponding protein, eIF2D, has been shown to
possess translation initiation factor activity;
GO_component: GO:0005840 - ribosome [Evidence IDA] [PMID
16702403]; GO_function: GO:0003723 - RNA binding [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence ISS]
[PMID 10093218]; GO_function: GO:0003743 - translation
initiation factor activity [Evidence IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0006413 - translational initiation [Evidence IEA]"
/codon_start=1
/product="Tma64p"
/protein_id="XP_018736366.1"
/db_xref="GeneID:30037016"
/translation="MQVHSGEKAVLYTDNDGQPVWIALEDGYLIPTLYTAWNAPFLMQ
RVCTVKYTLERLQSGADLMIPGIFDPLPENVPPGTVVSISLIDSPNIAVAVGIALVDC
SRGKLDTNQKGKAVQLINVIDDKLFQLYRKKVPVPIELDVSVPLLETQDTPGDAENDP
VTDKSKISAEGQDSIVNHTAESNVIENVNNGSSKETSQVEEITDTTENINLGENQPET
ETDGPENDTNSPSTAEIDDAFRRALLQTLRKAVDSPIEVPIPSSTFLSNYVLLNLPYN
HPSVQMKKTSWKKASKFFKVMEKQSLLKGKERGGEFVITSLAGKNNTEVSSFTLFKPR
PFEQHKPAASGSGTTSSNKKTYLSAKEYFKPRSMTAPIFEFSKLDPSKYYTADTIRQT
LNNYITGNSLSDPKNPKNVKLDRPLTKALGLSPTIESIGRDKISSLLQSNCSQFHHIT
SQNDEDLNRQTATTLAKGPIPRITITTEKRAGRKVVTNVTNIEPFHLDPIAFAEELRV
ACAGSTSVNAIKEGSDLQQVMIQGHHVAAITKTLELKGIRPAWIDVVDKARR"
gene complement(<696676..>698139)
/gene="GPI17"
/locus_tag="AWJ20_4841"
/db_xref="GeneID:30037017"
mRNA complement(<696676..>698139)
/gene="GPI17"
/locus_tag="AWJ20_4841"
/product="Gpi17p"
/transcript_id="XM_018881939.1"
/db_xref="GeneID:30037017"
CDS complement(696676..698139)
/gene="GPI17"
/locus_tag="AWJ20_4841"
/inference="similar to AA sequence:KEGG_Orthology:K05291"
/note="Transmembrane protein; subunit of the
glycosylphosphatidylinositol transamidase complex that
adds GPIs to newly synthesized proteins; human PIG-S
homolog; GO_component: GO:0042765 - GPI-anchor
transamidase complex [Evidence IEA]; GO_component:
GO:0042765 - GPI-anchor transamidase complex [Evidence
IDA] [PMID 15939668]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IDA] [PMID
15075373]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IDA] [PMID 15939668]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0003923 - GPI-anchor transamidase activity [Evidence
IMP,ISS] [PMID 11483512]; GO_process: GO:0006506 - GPI
anchor biosynthetic process [Evidence IEA,IEA];
GO_process: GO:0016255 - attachment of GPI anchor to
protein [Evidence IEA]; GO_process: GO:0016255 -
attachment of GPI anchor to protein [Evidence IMP,ISS]
[PMID 11483512]"
/codon_start=1
/product="Gpi17p"
/protein_id="XP_018736367.1"
/db_xref="GeneID:30037017"
/translation="MASEEAIGNIRRRRWIVLSYLLLGGLIGLPIWIWTTSIWRAPLS
YEEMDMYDHNIQDLINVKIPVYLNVGTEFPDLVKSTQLVVNRKLEDRGIKQWNLEILS
GRGDANSYNVAFRLGEDDEYIISEVSRDVLITYSIDSVSLGVVPELVSIILVDHLFAD
ELSLLEDAIGQKKQGLDNSDSSDKIVSYSPNYHLTFSLFYGGGDKISWDISQALDEHF
AHLKESYARISNLTIDTQVQYYSDLGCNIHHDEENNQWLLDHNDLSTFVNFAEWSLTS
IHSYPTLHFILYIPSPKQSPMIIVDSNTNSFSIPQWGGVQIWNKPEASKSHLTVSDLL
PILETFSSQFLGLVGAPVHPKSPVIRLDFLSRLSTIRALISASHTLGSLHRLSQSLPD
IAIPQPVLYAVKSALLAIQGSLYSLSTGQFTQAIKLAGDALRQSENAFFEKEMVQQAF
FPEEHKVAIYLPLLGPIGIVLLMGTLRCFKEFKTHGW"
gene <698456..>699388
/gene="MRPL1"
/locus_tag="AWJ20_4842"
/db_xref="GeneID:30037018"
mRNA <698456..>699388
/gene="MRPL1"
/locus_tag="AWJ20_4842"
/product="mitochondrial 54S ribosomal protein MRPL1"
/transcript_id="XM_018881940.1"
/db_xref="GeneID:30037018"
CDS 698456..699388
/gene="MRPL1"
/locus_tag="AWJ20_4842"
/note="Mitochondrial ribosomal protein of the large
subunit; GO_component: GO:0015934 - large ribosomal
subunit [Evidence IEA]; GO_component: GO:0005762 -
mitochondrial large ribosomal subunit [Evidence IPI] [PMID
12392552]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IEA]; GO_function: GO:0003735 - structural
constituent of ribosome [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IPI] [PMID 12392552]; GO_process: GO:0032543 -
mitochondrial translation [Evidence IC] [PMID 12392552];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="mitochondrial 54S ribosomal protein MRPL1"
/protein_id="XP_018736368.1"
/db_xref="GeneID:30037018"
/translation="MSLARSLLLRSGNAATTTAGLISVSSRPLLQLINPPQPRYGSKK
KRDEKKAAKSDGAPSKADAKQLAHQREKVLLRLRQKREETKKATKLRVEAKNASKSSN
FMDIETALRYLRAAEVGRSAHATTISLTMRIVAEKGAVPVNTKCRLPKPLDEERIAVF
TNSSELAAEARRAGAVLVGGDDIIDQVKQGIINFDKAYATPDIVSRLNQVARILGPKG
LMPNAKRGTVTHDIYNALVQASGEMTFKQEGTMLSLPVGRASFTDLEIVSNIVTVTDA
IRKFMTDSSAHNKKAYIGKTVITSTTGPSIVVEV"
gene <703912..>704730
/locus_tag="AWJ20_4843"
/db_xref="GeneID:30037019"
mRNA <703912..>704730
/locus_tag="AWJ20_4843"
/product="hypothetical protein"
/transcript_id="XM_018881941.1"
/db_xref="GeneID:30037019"
CDS 703912..704730
/locus_tag="AWJ20_4843"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736369.1"
/db_xref="GeneID:30037019"
/translation="MSKETAEYTHGHHESVLRSHTWRTVDNSVKFLVPYLKPDSYVLD
VGCGPGTITVDMAKNYTPKGKIIGMEYAGSVLEQAAAHAKENNVTNIEFAVGDVHNLS
YPDNTFDIVFAHQVVQHIGNPVVALKEMARVVKPGGVVAIRDSDYAGFFWYPELPALK
KWQEVYLKVAKANGAEPNAGRHYHSWAHQAGLDQPKLKLSSSTWFYWTPEERAWWSSL
WADRTLKSNFATTVLKHDLGTQDDLEEIAQGWRDWGKDKDAIFNLAHVELIYQK"
gene <705691..>707355
/gene="PGM2"
/locus_tag="AWJ20_4844"
/db_xref="GeneID:30037020"
mRNA <705691..>707355
/gene="PGM2"
/locus_tag="AWJ20_4844"
/product="phosphoglucomutase PGM2"
/transcript_id="XM_018881942.1"
/db_xref="GeneID:30037020"
CDS 705691..707355
/gene="PGM2"
/locus_tag="AWJ20_4844"
/inference="similar to AA sequence:KEGG_Orthology:K01835"
/note="Phosphoglucomutase; catalyzes the conversion from
glucose-1-phosphate to glucose-6-phosphate, which is a key
step in hexose metabolism; functions as the acceptor for a
Glc-phosphotransferase; protein abundance increases in
response to DNA replication stress; PGM2 has a paralog,
PGM1, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
8385141]; GO_component: GO:0005829 - cytosol [Evidence
IBA]; GO_function: GO:0016868 - intramolecular transferase
activity, phosphotransferases [Evidence IEA]; GO_function:
GO:0016853 - isomerase activity [Evidence IEA];
GO_function: GO:0000287 - magnesium ion binding [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0004614 -
phosphoglucomutase activity [Evidence IEA]; GO_function:
GO:0004614 - phosphoglucomutase activity [Evidence IMP]
[PMID 13887540]; GO_function: GO:0004614 -
phosphoglucomutase activity [Evidence IMP] [PMID 2138705];
GO_function: GO:0004614 - phosphoglucomutase activity
[Evidence IGI,IMP,ISS] [PMID 8119301]; GO_process:
GO:0006011 - UDP-glucose metabolic process [Evidence IGI]
[PMID 9252577]; GO_process: GO:0005975 - carbohydrate
metabolic process [Evidence IEA,IEA]; GO_process:
GO:0006874 - cellular calcium ion homeostasis [Evidence
IMP] [PMID 10681519]; GO_process: GO:0006874 - cellular
calcium ion homeostasis [Evidence IGI,IMP] [PMID
15252028]; GO_process: GO:0030003 - cellular cation
homeostasis [Evidence IMP] [PMID 15164360]; GO_process:
GO:0019388 - galactose catabolic process [Evidence IMP]
[PMID 13887540]; GO_process: GO:0019388 - galactose
catabolic process [Evidence IMP] [PMID 14264884];
GO_process: GO:0019388 - galactose catabolic process
[Evidence IGI,IMP] [PMID 8119301]; GO_process: GO:0019255
- glucose 1-phosphate metabolic process [Evidence IMP]
[PMID 10681519]; GO_process: GO:0019255 - glucose
1-phosphate metabolic process [Evidence IGI,IMP,ISS] [PMID
8119301]; GO_process: GO:0051156 - glucose 6-phosphate
metabolic process [Evidence ISS] [PMID 8119301];
GO_process: GO:0006006 - glucose metabolic process
[Evidence IEA]; GO_process: GO:0005978 - glycogen
biosynthetic process [Evidence IGI] [PMID 9252577];
GO_process: GO:0005992 - trehalose biosynthetic process
[Evidence IGI] [PMID 9252577]"
/codon_start=1
/product="phosphoglucomutase PGM2"
/protein_id="XP_018736370.1"
/db_xref="GeneID:30037020"
/translation="MTVVSTKAFEGQKPGTSGLRKKVTVFQQPHYTENFVQAILSSIP
EGSEGATLVVGGDGRFYNKEVIQIISEISAANGVKKLIIGQNGLLSTPAASHVIRKRK
ATGGIILTASHNPGGPTNDFGIKYNLANGGPAPESVTDKIYEVTKTISEYKTLKGADF
PQVNLSKIGAATYGPLEVEIIDTVEDYLLLVKEIFDFDLIKSFLKSNPDFTILFDALN
GITGPYGKAIFIDELGLAASSVQNAVPKTDFGGLHPDPNLTYAHTLVERVDKEKTPFG
AASDGDGDRNMIYGANSFVSPGDSVAIIAHHADKIPYFRNGGVNGLARSMPTSGAIDL
VAKAKGLKCYEVPTGWKFFTALFDSKQLSICGEESFGTGSDHIREKDGLWAIVAWLNI
IAGVAKEDPSKASIAAIQHDFWNTYGRTFFTRYDYENVSSEGAAKVVANLKSQIESPS
FVGSKVDNLEVTEAGDFSYTDLDGSVSKNQGLYVKFKGGSRIVVRLSGTGSSGATIRL
YIERHESDKSLFSQDAQDYLKETISSAVNFLKLKEFVNREEPDVRT"
gene complement(<707575..>709068)
/gene="YKU80"
/locus_tag="AWJ20_4845"
/db_xref="GeneID:30037021"
mRNA complement(<707575..>709068)
/gene="YKU80"
/locus_tag="AWJ20_4845"
/product="Yku80p"
/transcript_id="XM_018881943.1"
/db_xref="GeneID:30037021"
CDS complement(707575..709068)
/gene="YKU80"
/locus_tag="AWJ20_4845"
/inference="similar to AA sequence:KEGG_Orthology:K10885"
/note="Subunit of the telomeric Ku complex
(Yku70p-Yku80p); involved in telomere length maintenance,
structure and telomere position effect; required for
localization of telomerase ribonucleoprotein via
interaction with the TLC1 guide RNA; relocates to sites of
double-strand cleavage to promote nonhomologous end
joining during DSB repair; GO_component: GO:0043564 -
Ku70:Ku80 complex [Evidence IEA]; GO_component: GO:0043564
- Ku70:Ku80 complex [Evidence IDA] [PMID 8754818];
GO_component: GO:0005694 - chromosome [Evidence IEA];
GO_component: GO:0000781 - chromosome, telomeric region
[Evidence IEA,IEA]; GO_component: GO:0000790 - nuclear
chromatin [Evidence TAS] [PMID 10367891]; GO_component:
GO:0005724 - nuclear telomeric heterochromatin [Evidence
TAS] [PMID 12080091]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA,IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0004003 -
ATP-dependent DNA helicase activity [Evidence IEA];
GO_function: GO:0003677 - DNA binding [Evidence IEA,IEA];
GO_function: GO:0003677 - DNA binding [Evidence IDA] [PMID
16166630]; GO_function: GO:0003723 - RNA binding [Evidence
IDA] [PMID 12975323]; GO_function: GO:0003684 - damaged
DNA binding [Evidence IEA]; GO_function: GO:0003684 -
damaged DNA binding [Evidence TAS] [PMID 10367891];
GO_function: GO:0004386 - helicase activity [Evidence
IEA]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_function: GO:0042162 -
telomeric DNA binding [Evidence IEA]; GO_function:
GO:0042162 - telomeric DNA binding [Evidence IDA] [PMID
17656141]; GO_process: GO:0032508 - DNA duplex unwinding
[Evidence IEA]; GO_process: GO:0006310 - DNA recombination
[Evidence IEA,IEA]; GO_process: GO:0006281 - DNA repair
[Evidence IEA]; GO_process: GO:0006974 - cellular response
to DNA damage stimulus [Evidence IEA]; GO_process:
GO:0006333 - chromatin assembly or disassembly [Evidence
IDA] [PMID 11553718]; GO_process: GO:0006342 - chromatin
silencing [Evidence IDA,IMP] [PMID 9501103]; GO_process:
GO:0007535 - donor selection [Evidence IDA,IMP] [PMID
16166630]; GO_process: GO:0007535 - donor selection
[Evidence IGI,IMP] [PMID 16809780]; GO_process: GO:0000724
- double-strand break repair via homologous recombination
[Evidence IMP] [PMID 11016833]; GO_process: GO:0006303 -
double-strand break repair via nonhomologous end joining
[Evidence IEA]; GO_process: GO:0034502 - protein
localization to chromosome [Evidence IGI] [PMID 18716325];
GO_process: GO:0000723 - telomere maintenance [Evidence
IEA]; GO_process: GO:0000723 - telomere maintenance
[Evidence IMP] [PMID 10818099]"
/codon_start=1
/product="Yku80p"
/protein_id="XP_018736371.1"
/db_xref="GeneID:30037021"
/translation="MICLITNGTGEMDFKKVPDLVKRLKAANIVLRILMIGMKDSGDE
LTTFEKEIRQSNIEELQNIERDYYPPDSDGRPDDMYMFAPYEEAYFYVSKPVVQPVGV
LRQFKGVLRLGRPTEDSESVKHVIDEENTITIPVYGYPCTRRTRVPTAKSVIVKRKRP
DDDGGESEDDEFNDRKVTVHFVRDYYVEKVKDENGEGEQNPDNRIILERQNLLKGYRF
GSEIVTFDAETKKELSSIHLSNSPELSIYGFCSAKDVPRWYLMEASDYIVVSPEARKK
DQLAMSAMILTLQLDDRVAIARYVAKPEKDAHMVLLSPYVDVDFQALILNILPFSQDY
RKFQFPSLLEIKSKSGKILPPDNPQRINREPTTEMNDLMSHYIDEMDLEHAGEPDEDG
EPTEYMTCEDTFNPVLHRLRNAIKVAGLTGAKNFVPPVWPELVKYSHPPAGRLDQLKP
TTDRLSQLLDIKVVETKKSEKQKVKQEAEASKRVATDTDIKLEDLLS"
gene complement(<709547..>711244)
/gene="GAL7"
/locus_tag="AWJ20_4846"
/db_xref="GeneID:30037022"
mRNA complement(join(<709547..709550,709779..709792,
710159..>711244))
/gene="GAL7"
/locus_tag="AWJ20_4846"
/product="UDP-glucose:hexose-1-phosphate
uridylyltransferase"
/transcript_id="XM_018881944.1"
/db_xref="GeneID:30037022"
CDS complement(join(709547..709550,709779..709792,
710159..711244))
/gene="GAL7"
/locus_tag="AWJ20_4846"
/inference="similar to AA sequence:KEGG_Orthology:K00965"
/note="Galactose-1-phosphate uridyl transferase;
synthesizes glucose-1-phosphate and UDP-galactose from
UDP-D-glucose and alpha-D-galactose-1-phosphate in the
second step of galactose catabolism; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 10993714];
GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
uridylyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
uridylyltransferase activity [Evidence IMP] [PMID
14158615]; GO_function: GO:0008108 -
UDP-glucose:hexose-1-phosphate uridylyltransferase
activity [Evidence IDA] [PMID 387754]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0016779 - nucleotidyltransferase
activity [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0005975 - carbohydrate metabolic process [Evidence
IEA]; GO_process: GO:0033499 - galactose catabolic process
via UDP-galactose [Evidence IMP] [PMID 14158615];
GO_process: GO:0033499 - galactose catabolic process via
UDP-galactose [Evidence IDA] [PMID 387754]; GO_process:
GO:0006012 - galactose metabolic process [Evidence
IEA,IEA,IEA]"
/codon_start=1
/product="UDP-glucose:hexose-1-phosphate
uridylyltransferase"
/protein_id="XP_018736372.1"
/db_xref="GeneID:30037022"
/translation="MQWSEAHRRYNPLRKSWVLCSPHRTQRPWQGQQEKPQVEKRPEY
DEKCYLCPGNTRAQGQANPKYEETFVFVNDFSAVKPRDTLPQADGSSAEESEEDELFR
AEQATGKCVVICFNPRHDITLAQMTYPQVSGVINAWKKVYTDAVNNPEVRYCQIFENK
GAAMGCSNPHPHGQAWTTNIIPEEPDIEHACLAEYAHNHNGKGLLEDYVAKELAILAK
AESSGNHAQNRFVDVNDSFIAVIPFWATWPFEVLVVSRRKIGNITQLTEKEQTDFAHI
LRNVALRYDNLFQTSFPYSMGIHQSFAEPNIDPKSEPEHLHVHFYPPLLRSATVRKFL
VGFEMLGMPQRDVTPESAAAQLRAVDGNATVFV"
gene complement(<711932..>712681)
/gene="SLD5"
/locus_tag="AWJ20_4847"
/db_xref="GeneID:30037023"
mRNA complement(<711932..>712681)
/gene="SLD5"
/locus_tag="AWJ20_4847"
/product="Sld5p"
/transcript_id="XM_018881945.1"
/db_xref="GeneID:30037023"
CDS complement(711932..712681)
/gene="SLD5"
/locus_tag="AWJ20_4847"
/inference="similar to AA sequence:KEGG_Orthology:K10735"
/note="Subunit of the GINS complex (Sld5p, Psf1p, Psf2p,
Psf3p); complex is localized to DNA replication origins
and implicated in assembly of the DNA replication
machinery; GO_component: GO:0031261 - DNA replication
preinitiation complex [Evidence IGI,IMP] [PMID 12730134];
GO_component: GO:0000811 - GINS complex [Evidence IPI]
[PMID 12730134]; GO_component: GO:0000811 - GINS complex
[Evidence IPI] [PMID 12768207]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0031298 -
replication fork protection complex [Evidence IDA] [PMID
16531994]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006260 - DNA replication
[Evidence IEA]; GO_process: GO:0006261 - DNA-dependent DNA
replication [Evidence IMP] [PMID 12730134]; GO_process:
GO:0006261 - DNA-dependent DNA replication [Evidence IMP]
[PMID 12768207]; GO_process: GO:0000727 - double-strand
break repair via break-induced replication [Evidence IMP]
[PMID 20516198]"
/codon_start=1
/product="Sld5p"
/protein_id="XP_018736373.1"
/db_xref="GeneID:30037023"
/translation="MNDTTDIDDIINNLRDDDISNLHSGIEIQSSLDDSFQQTDLTDL
KKAWLAERMSPDILAFQTSLIDRLMDRIRIQAAYIEENSMADFHNSGDIKMKLLIVES
ELERIKFLVRGYLRTRLSKIDKYTIYIIQSGENQQEGNADQFDLRLLLSPQEIQYMER
HHRILLNLYNTQFLSALPAQLQRLDDSSGGISMVEEPDLDKPVFIRVLETVERPIRVG
LDEITLEKGNIYVLRYSAISKYLIGGEVELI"
gene <713518..>715242
/locus_tag="AWJ20_4848"
/db_xref="GeneID:30037024"
mRNA <713518..>715242
/locus_tag="AWJ20_4848"
/product="hypothetical protein"
/transcript_id="XM_018881946.1"
/db_xref="GeneID:30037024"
CDS 713518..715242
/locus_tag="AWJ20_4848"
/inference="similar to AA sequence:KEGG_Orthology:K10415"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736374.1"
/db_xref="GeneID:30037024"
/translation="MKDAGTDTSLDMVVTEVFEIAPKSTTISYNKEVQTSEDWASGTN
TKSASGIEDYDEFDYSVNMAQRKQLEDKIRTELEELYKKENEEVLKNNRTLSNDEPGL
NANGTPDVSRPTILSDKEASQISARQDFENFWSQSWKVINRAAEEDTNYNILRNYAES
QNSDAQQQSRNSLTEITHLYSKDSVGRAVTYIDWSPKFPELIAAAYTEKSSDPHATKG
LIQIFNLHAPNQPEYTLTAQSDILVVKFSPFDSNLIFGAAYNGQVLAWDLRSKGSRPL
LSSQLSGKAHTHPVYSLEIIGTQNAYTLITASTDGTVCTWTPDMLAKPQEKITLQTNS
SSLRNDEAAPTSLAIPTRDQAQFLIGTEEGAIYQCYRFGQAGSRPGIDHRKSYRGHTG
PVTSVDLHPSSGPVDFGNYLLSSGNDWSIKIWLVSLISSHGSNLSSTASSAPANRGEI
IRPILDIKRDDVVYDISWNPNHPSIFAAVDGTGHVEVWNLLTDTEVPVVRAKPSNIEK
NIVGSNYLKRPLNRVSWEKTSEARRLAVGGLNGSITILELDTTLIGSSPKPEHWAQLK
RLFEQLES"
gene complement(<715649..>717979)
/locus_tag="AWJ20_4849"
/db_xref="GeneID:30037025"
mRNA complement(<715649..>717979)
/locus_tag="AWJ20_4849"
/product="hypothetical protein"
/transcript_id="XM_018881947.1"
/db_xref="GeneID:30037025"
CDS complement(715649..717979)
/locus_tag="AWJ20_4849"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736375.1"
/db_xref="GeneID:30037025"
/translation="MVKQPYRGVRPRPLKLNRIGRSAFDDIHLLGGIGHKWRIGGDSG
NPVNSGSKSGYSSDPESESSRSSWPYFNRSLFKVSSPTAALNMIPKWMKAVKLSVPSS
SRAYGWWHTSNWHPLLNHKPHASHSMPDPIFESVWSCKKPQVTYHEFKYHPRTGGKGR
SAIWLKYNISNESSLRNLREEEERYQKEQESTSNETDYDAIDLISLKKKVRTDESQNA
AYSVEPVDTINASETPLSTRGGGSRKAEMASGESSKSGGGLGSGDNSGGDGRGKKQPP
PVTLQSDPTAQLSKKQRKRARAAAAAAQASNNFTAKDIDETLEASTDELYQTGADVES
KPLSQLRKPIQVPTESMEQPEVSSWSSESVPVEPLANETVNNGVETDALSSDSINPIT
SSTVSDPLHDNSSASDRLSTGQVYDSTTLSESESNESPEISSKVDEDETSHVSTNGTT
GFPLHTASVDATENITLPTEPVSPVEHKSESTGGFDLHANAYPVDNEKNIHDTFSPLS
GPDSQPSGPHRIDTVTAGDLLEGLQLKGKYKDSTKDFERLLSLVETEKHDSEKRKSFT
NPVRITREGESPSDVENLFNEPKPISTFAPPLEMQDIHHLQDALKEAKADEEKHAQEM
FRIENAMDYTKSVSSKRPRSIESGNFFTPVNRTIASATHRKPGAAYEYIIVTPNQKFI
RTRTLPFKDDNPRRDLFSALSEMSDPALYVKTIAKLEKANWKCIGGGGPGRLLVFERE
YNPIKRFSKLLLKLTGSFLGLVSLAAAVAVMIESPF"
gene <719318..>720154
/gene="MUM2"
/locus_tag="AWJ20_4850"
/db_xref="GeneID:30037027"
mRNA <719318..>720154
/gene="MUM2"
/locus_tag="AWJ20_4850"
/product="Mum2p"
/transcript_id="XM_018881949.1"
/db_xref="GeneID:30037027"
CDS 719318..720154
/gene="MUM2"
/locus_tag="AWJ20_4850"
/note="Protein essential for meiotic DNA replication and
sporulation; cytoplasmic protein; subunit of the MIS
complex which controls mRNA methylation during during the
induction of sporulation; also interacts with Orc2p, which
is a component of the origin recognition complex;
GO_component: GO:0036396 - MIS complex [Evidence IDA,IPI]
[PMID 22685417]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005634 -
nucleus [Evidence IEA]; GO_function: GO:0005515 - protein
binding [Evidence IPI] [PMID 22685417]; GO_process:
GO:0080009 - mRNA methylation [Evidence IGI,IMP] [PMID
22685417]; GO_process: GO:0007126 - meiotic nuclear
division [Evidence IEA]; GO_process: GO:0007126 - meiotic
nuclear division [Evidence IMP] [PMID 22685417];
GO_process: GO:2000221 - negative regulation of
pseudohyphal growth [Evidence IMP] [PMID 22685417];
GO_process: GO:0006279 - premeiotic DNA replication
[Evidence IGI] [PMID 11238403]; GO_process: GO:0006279 -
premeiotic DNA replication [Evidence IMP] [PMID 9504908];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="Mum2p"
/protein_id="XP_018736376.1"
/db_xref="GeneID:30037027"
/translation="MNAQLSRYTAGNQAGQGTNSQTGQTGQGQNNGHQGKMISTGGNV
NTSNQYSSTNFYNSLTPKSSFDSIGSLHSLGSNVSSVPVGLASNPWVSGSFGSFSIPP
PLPPSSGRSENSNSYHDSFNFAPADLSFVNDIDPPNEYSSSPSLNLSNAQKQPFKTLN
NKPRGNHNNNSLGNSQFSNASTHQLHLDPVIENEFRNMAKSLEEKEALISDLKLQLES
LVTAVAVCGSVNDKAVQEVISEMKMDATEIAHRITIRLKTLKSENEQLGVSNIIIRFT
VC"
gene complement(<720467..>722287)
/locus_tag="AWJ20_4851"
/db_xref="GeneID:30037028"
mRNA complement(<720467..>722287)
/locus_tag="AWJ20_4851"
/product="hypothetical protein"
/transcript_id="XM_018881950.1"
/db_xref="GeneID:30037028"
CDS complement(720467..722287)
/locus_tag="AWJ20_4851"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736377.1"
/db_xref="GeneID:30037028"
/translation="MEGLEFASNFSFNRCSPQLSASGEYVAYVYGSRLVIRFTDTLAT
KNVIQLPREFAQNIFLIRWDGCLKHSPEQRVAVATRNEIRTFSIKKHSTAGESMAPDN
LAGHSVILSGKDEIANVQWLSADTPNNIGQIVRLAVFTRFHLDVKIWSSNGVEMEIRS
PKFARLVSETPNLSMDGLSNRFSVVSRFTTYDVLYNCRINATVADNELVTPLSEALDI
VDLKWSPSGLWLAAIDNPIAGYDVHLWAADGTFVRTFHDIPGFISPNVNTTPSSRRKE
DMLRDTPSAAFPTCLEWVNVFSDPGVRDLELLLVGDSENRVSLFDIKTCQPTSILIHG
SVYKNCCLWEEYLSPDSHQFEYRMVRLPFIFTNSGANTSNSKMNEHERNIISGVISMD
ISDNGQLLATRVHSYPNLVWIWSIANPQSPDLLAIISHSYTIKSITWKSESMTPLLLI
TPLDSKSIGLWSYDSQLEPKIIDLSSQFESSIMGVSIIEKPDSNEILRLLAWTKNSFS
IGGSVQDENDTGDAEDEGQDANDMVTKQQNSKYTSYQRSSVPRLNHHQSSRMELLSPT
LPQDDDTHVKLLADIVQQSEWIQDTNSVPVEDTFHNKIKH"
gene complement(<722631..>723776)
/gene="ARO3"
/locus_tag="AWJ20_4852"
/db_xref="GeneID:30037029"
mRNA complement(<722631..>723776)
/gene="ARO3"
/locus_tag="AWJ20_4852"
/product="3-deoxy-7-phosphoheptulonate synthase ARO3"
/transcript_id="XM_018881951.1"
/db_xref="GeneID:30037029"
CDS complement(722631..723776)
/gene="ARO3"
/locus_tag="AWJ20_4852"
/inference="similar to AA sequence:KEGG_Orthology:K01626"
/note="3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP)
synthase; catalyzes the first step in aromatic amino acid
biosynthesis and is feedback-inhibited by phenylalanine or
high concentration of tyrosine or tryptophan;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0003849 -
3-deoxy-7-phosphoheptulonate synthase activity [Evidence
IEA,IEA]; GO_function: GO:0003849 -
3-deoxy-7-phosphoheptulonate synthase activity [Evidence
IDA] [PMID 2880280]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0009073 - aromatic amino acid family biosynthetic
process [Evidence IEA,IEA]; GO_process: GO:0009058 -
biosynthetic process [Evidence IEA]; GO_process:
GO:0008652 - cellular amino acid biosynthetic process
[Evidence IEA]; GO_process: GO:0009423 - chorismate
biosynthetic process [Evidence IEA]; GO_process:
GO:0009423 - chorismate biosynthetic process [Evidence
TAS] [PMID 1943992]; GO_process: GO:0006950 - response to
stress [Evidence IEA]"
/codon_start=1
/product="3-deoxy-7-phosphoheptulonate synthase ARO3"
/protein_id="XP_018736378.1"
/db_xref="GeneID:30037029"
/translation="MFLDNTHLGDRSRLEDWRIRGYNPLVSPDLLQHDIPLTTKSKEN
IVKGREDTCKILDGKDDRLLVVVGPCSIHDPKAALEYCQKLASVVPELSSDLHIVMRA
YLEKPRTTVGWKGLINDPDIDGSFQINKGLRISRELFVKLTEQLPIAGEMLDTISPQF
LSDLFSVGAIGARTTESQLHRELASGLSFPIGFKNGTDGGLDVAVDAVRAAANPHHFL
SVTKPGVVAIVGTDGNDDSFVILRGGKNGTNFDAASVAAAKEKLEKAGLVTDSRPGPR
IMIDCSHGNSSKNHNNQPIVAEAVAEQIRNGETGICGVMIESNINAGRQNVPPAEEGG
KDALKYGVSITDACIDWETTYKTLKNLAEAVRTRREANSHKKAKIDA"
gene <725223..>726275
/locus_tag="AWJ20_4853"
/db_xref="GeneID:30037030"
mRNA <725223..>726275
/locus_tag="AWJ20_4853"
/product="hypothetical protein"
/transcript_id="XM_018881952.1"
/db_xref="GeneID:30037030"
CDS 725223..726275
/locus_tag="AWJ20_4853"
/inference="similar to AA sequence:KEGG_Orthology:K16576"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736379.1"
/db_xref="GeneID:30037030"
/translation="MSSSQSRSVPLVNRSPAKRPVSSTPRSPLASPNKSPSKSRSGRL
SRAVSRLSVSDSIDIFESPSVLLEISQTVVKAGFPGDAVPQVTYHTDIPLWNRPDDES
ADIPPVRLYLLNVLQEILIQSLLVTPKSISVFVLESPLISNTFKQLIGQVLLLDLQVK
SVTFQPLAVNAVVGAGASCGLVIDLGWDEAAMIPVYDLRELSSKARFTTRSTKKLFHK
YRDTALAESKPFTTWREFRHQTLASTFSTSEITELAQELFIKQNEPYPDDDNLSLPLV
ISKLIGSLDANLRGPCSENIVFTGGLQLPPEIYISIINEVQTSWPKARLIHSIGSWAG
ASAYLSSIIAYHSQLH"
gene complement(<726495..>728198)
/gene="PWP1"
/locus_tag="AWJ20_4854"
/db_xref="GeneID:30037031"
mRNA complement(<726495..>728198)
/gene="PWP1"
/locus_tag="AWJ20_4854"
/product="Pwp1p"
/transcript_id="XM_018881953.1"
/db_xref="GeneID:30037031"
CDS complement(726495..728198)
/gene="PWP1"
/locus_tag="AWJ20_4854"
/inference="similar to AA sequence:KEGG_Orthology:K14791"
/note="Protein with WD-40 repeats involved in rRNA
processing; associates with trans-acting ribosome
biogenesis factors; similar to beta-transducin
superfamily; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005730 - nucleolus [Evidence IPI] [PMID 15588312];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006364 - rRNA processing
[Evidence IMP,ISS] [PMID 15588312]"
/codon_start=1
/product="Pwp1p"
/protein_id="XP_018736380.1"
/db_xref="GeneID:30037031"
/translation="MISATSWVPRGFAAEFPVKYDLNDEEVERISHLAKLQLKDAQEA
LEEAEAEGADVNADADAEVTEAADENVDVAVKSEKKSVKFNASNEDGDAEVEEDDDLK
EYDLENYDNDEDEGEGEKMGMFNGVQSLAYYEPGEKDPYITLPNAEDEDEEREELQIL
PSDNLILATKTEDDISYLEVYVYNENSESKNDEEDAIDEPTLYVHHDLMLPSFPLCVE
WIGYQAGNKVQASGPDRVGNFAAIGTFEPEIEVWNLDVVDSAYPDLILGQRPDNANDH
LSVGPSKKSKKKQKKINSKLRNDQYHVDAVLSLSANKLHRNLLASGSADTTVKLWDLN
NGVCAKSFQFHDNKVSTVAFNPVEGSILLSGGYDATAIVSDLRVSGSEGQRKWKVDGD
IEGGLWASNGNEFYISTEHGRIHKFDARQEGKSLWTLQAHDDGITSFDVNKFIDGYMV
TASTDKSTKLWNLSPASGPSLILSRDLDIGKVFSVGFAPDKEAVGHVVAAGSQGIVRV
WDTLSNRTVRGSFGKQVSTLRKESAKEVIVGVTEDDEEEDDDEAGADAAENGGDSDEE
M"
gene <730157..>732340
/gene="MSS4"
/locus_tag="AWJ20_4855"
/db_xref="GeneID:30037032"
mRNA <730157..>732340
/gene="MSS4"
/locus_tag="AWJ20_4855"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018881954.1"
/db_xref="GeneID:30037032"
CDS 730157..732340
/gene="MSS4"
/locus_tag="AWJ20_4855"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018736381.1"
/db_xref="GeneID:30037032"
/translation="MGLRKLCRKFRHPFERDESVITTHHSFNDGVVASKSMPPDHLRT
RQNSSVEGIRSSYPNCQAFLAKYGTKDQAPRDNSRIGIIENPRNSYPNCEKLAYYSKR
SACGNLAQTGSTSRPYRHKDFDLIKTPQSPTGSIDSKKTLNPETFWPTTVVNHLIFEQ
NGYEEFIAKGMNSDLGTKGNNSEFEMDSTHSSGAENLHLIWSTPSQVSLVTVDQSSTS
SVSTVCSEEIVHQNVSQITEQVIKASNASDSLDSEETLHSLVNHGDKPVCEVVTMPSN
NDTDGSCTSESSQNFSELTKEESTSDKPTSPPVRPVRPRVCPFLLQPEIDEMIAKERG
ELVTSSVLRCGQSNLSDTYELPSATSASILEAATREYPPRFGSVDAALNPEVVKFDED
KKTMLMKRAQAKIDSLKRKLGLKSNPVAPKKYLIFLGIYSSLVENPPIIHPEVRVQFF
LPGHKTFNVSPDAVVQCHFPSVYENIRTLRGSYFPEYLNSFLFDHHALHVKSPGKTDS
DFYFTADRKYVIKTIQRKEHNTLMGESFMKDYYSHIQENPESLIPTYLGHFTLRTLGK
DTHFVVMKNLVGTDVDTVYDLKGIKHRKRSEATSAMNGRDINKDGDWVENQEIIFMGK
DKRKKLLTQLASDVALLEKYNIMEYSLLVGIHKDPQSSEIRHAMGMIDSLSPYGVSKA
LKSNISRIVHHHSSLNIINPRDYGKRFLNFIGSTVVLERGRPMTY"
gene <734400..>735878
/gene="NMT1"
/locus_tag="AWJ20_4856"
/db_xref="GeneID:30037033"
mRNA <734400..>735878
/gene="NMT1"
/locus_tag="AWJ20_4856"
/product="glycylpeptide N-tetradecanoyltransferase NMT1"
/transcript_id="XM_018881955.1"
/db_xref="GeneID:30037033"
CDS 734400..735878
/gene="NMT1"
/locus_tag="AWJ20_4856"
/inference="similar to AA sequence:KEGG_Orthology:K00671"
/note="N-myristoyl transferase; catalyzes the
cotranslational, covalent attachment of myristic acid to
the N-terminal glycine residue of several proteins
involved in cellular growth and signal transduction;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
1544917]; GO_function: GO:0004379 - glycylpeptide
N-tetradecanoyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0004379 - glycylpeptide
N-tetradecanoyltransferase activity [Evidence IMP] [PMID
8955162]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_function: GO:0016746 - transferase
activity, transferring acyl groups [Evidence IEA];
GO_process: GO:0018008 - N-terminal peptidyl-glycine
N-myristoylation [Evidence TAS] [PMID 10570244];
GO_process: GO:0006499 - N-terminal protein myristoylation
[Evidence IEA]; GO_process: GO:0001302 - replicative cell
aging [Evidence IMP] [PMID 10921902]"
/codon_start=1
/product="glycylpeptide N-tetradecanoyltransferase NMT1"
/protein_id="XP_018736382.1"
/db_xref="GeneID:30037033"
/translation="MSGSEKTESREESAGASQPSEVVAKGNAGSNSGKPVTAVSSADT
KPPKTTGMRLEDLFKLLSMPDPKAEPKPMDEYKFWKTQPVTKFDDAIDAEGPIDSKTV
DDVSKEPSPLPAGFEWSTIDISQPDKVQEIYDLLYANYIEDDDETFRFKYSPAFLDWG
LKPPGWVKDWNVGVRVKDTGKLIGFISGIPGTLRLRTTNPDIKVAEINFLCVHKKLRS
KRLAPVLIREVTRRVNLTGVWQALYTAGALLPSPITTARYYHRSINWPKLHDVGFTGL
PSNSTPAKQVAKYALPSKPTLETLRPMTVEDVSEVHELLDTFLNRYELVPKFTEQETA
HWLLDSVHASEHDKVIYSYVVTDSENKITDFVSFYGLESTVLGNSKYDSINVAYLFYY
ATTAGLGDNKSSDNESVLKSRLFVLIQNALILAKAKNFDVFNALSVQDNALFLEDLKF
GAGDGFLNYYLFNYKAFPIHGGISKREKSLERKPGGIGVIMF"
gene complement(<736235..>736729)
/gene="COX5A"
/locus_tag="AWJ20_4857"
/db_xref="GeneID:30037034"
mRNA complement(<736235..>736729)
/gene="COX5A"
/locus_tag="AWJ20_4857"
/product="cytochrome c oxidase subunit Va"
/transcript_id="XM_018881956.1"
/db_xref="GeneID:30037034"
CDS complement(736235..736729)
/gene="COX5A"
/locus_tag="AWJ20_4857"
/inference="similar to AA sequence:KEGG_Orthology:K02263"
/note="Subunit Va of cytochrome c oxidase; cytochrome c
oxidase is the terminal member of the mitochondrial inner
membrane electron transport chain; Cox5Ap is predominantly
expressed during aerobic growth while its isoform Vb
(Cox5Bp) is expressed during anaerobic growth; COX5A has a
paralog, COX5B, that arose from the whole genome
duplication; GO_component: GO:0016021 - integral component
of membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence
IEA,IEA]; GO_component: GO:0005751 - mitochondrial
respiratory chain complex IV [Evidence IPI] [PMID
1331058]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0004129 -
cytochrome-c oxidase activity [Evidence IEA,IEA];
GO_function: GO:0004129 - cytochrome-c oxidase activity
[Evidence IMP] [PMID 1847916]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_process:
GO:1902600 - hydrogen ion transmembrane transport
[Evidence IEA,IEA]; GO_process: GO:0006123 - mitochondrial
electron transport, cytochrome c to oxygen [Evidence IDA]
[PMID 1331058]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]"
/codon_start=1
/product="cytochrome c oxidase subunit Va"
/protein_id="XP_018736383.1"
/db_xref="GeneID:30037034"
/translation="MLRGFRSVARPSLARAAAPVARRQAHAISNPTLVNLESRWEAMS
EEERSDIVAQLAERQKGSWAELTVAEKRAAWYISYGAWGPRKPIHAPGDAGRIVKGIA
AVILGAATLFTTIRLLSPSLPHTMNKEWQEAANEKLAEKNANPFTGYNQVQSPSKGPS
DDDE"
gene <740999..>742726
/gene="MUP1"
/locus_tag="AWJ20_4858"
/db_xref="GeneID:30037035"
mRNA <740999..>742726
/gene="MUP1"
/locus_tag="AWJ20_4858"
/product="Mup1p"
/transcript_id="XM_018881957.1"
/db_xref="GeneID:30037035"
CDS 740999..742726
/gene="MUP1"
/locus_tag="AWJ20_4858"
/note="High affinity methionine permease; integral
membrane protein with 13 putative membrane-spanning
regions; also involved in cysteine uptake; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0005887 - integral component of plasma membrane
[Evidence ISS] [PMID 8893857]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA,IEA]; GO_function: GO:0000102 -
L-methionine secondary active transmembrane transporter
activity [Evidence IDA] [PMID 8893857]; GO_function:
GO:0015171 - amino acid transmembrane transporter activity
[Evidence IEA]; GO_process: GO:0003333 - amino acid
transmembrane transport [Evidence IEA]; GO_process:
GO:0006865 - amino acid transport [Evidence IEA];
GO_process: GO:0042883 - cysteine transport [Evidence IDA]
[PMID 11330701]; GO_process: GO:0044690 - methionine
import [Evidence IGI,IMP] [PMID 8893857]; GO_process:
GO:0015821 - methionine transport [Evidence IDA] [PMID
11330701]; GO_process: GO:0000101 - sulfur amino acid
transport [Evidence IMP] [PMID 8893857]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Mup1p"
/protein_id="XP_018736384.1"
/db_xref="GeneID:30037035"
/translation="MASETSSLLSRQTGSQQHLEVGAVSNHANINRGNNSNASGNSSD
SGKGNGNSNGKLYREDHMEEEFDTQTIASYDSVYNSDIDNGVPFDPEHGHAHRKTLGI
PTATFLMLNKMIGTGIFSIPSSVYELTGSVGWSLVLWIFGGLIAYSGLNVYLEFGVEI
PKSGSEKNYLERVFRRPKHLALVTFAVSYLCLSHSTSNSYAFGLYIQLAAGVKETSES
YTRVIAVITVLSCILLHGIFPRAGRYLFNALGFFKVVVLGIITMSGFLVLIGAIKLDN
PPDNFTNMFQNDGMGGDVYSISIALLRVFFSYRGWDNCNTVMGEIRNPAHTMGIAGPL
AIGMVMFFYCFCNIAYFAVVSKQEIADSGVIVAGKFCQIIFGDSVAARVLPFFIALSN
LGNILVVSYAASRVTAELGKHYLIPFSPIISSYKPFNTPFVGLLMHASLTVILLVVPP
PGRVYEFVVDLSTYPITFISFSVTAGLIYLQFNRVKENWGNKPGSYHAPLYHSVAYLL
TNLFLIIVPWIPPPYELKPGSFPYYAVPLGGIIIMTAGPLYWLHWRNSENAARLDAEI
EKGLYLQTS"
gene complement(<742984..>745986)
/gene="PSK2"
/locus_tag="AWJ20_4859"
/db_xref="GeneID:30037036"
mRNA complement(<742984..>745986)
/gene="PSK2"
/locus_tag="AWJ20_4859"
/product="serine/threonine protein kinase PSK2"
/transcript_id="XM_018881958.1"
/db_xref="GeneID:30037036"
CDS complement(742984..745986)
/gene="PSK2"
/locus_tag="AWJ20_4859"
/inference="similar to AA sequence:KEGG_Orthology:K08286"
/codon_start=1
/product="serine/threonine protein kinase PSK2"
/protein_id="XP_018736385.1"
/db_xref="GeneID:30037036"
/translation="MLNELRKTLIDNQAHSSQQQLHQQHQQQQQQKQPNILSLNQLLM
ADQAFGSLHGSASTATLSILGNLTSSSLKTAANGGQLNSNLVNEHLDAFSALLDEAAE
LLPVPIDSHGGAGWSSTMPRSVSNPPLILQGSPVVNTTGSIAAIGSGVTGGATPSIGA
TSGSTDLRAVLDLESAKKKRVAAGLGESSPQLQNQLLSALSMAYIDMPRPLVFQRGHS
TVNFASITNSVSTLNLAILPSSAQGNNSSSVNITPVQPISSKYASNYSVFTTEFKSPY
KILSANDMACLLLGLSQVDLKKRSIFDILEDNYGQYVENIINNGLNGGQPKSVFICGH
ILPIVKSNGIHSLASFWVRSHNGFISWVLNEVIADKIYLKVSTLDWKLLSITGDNYKI
FTPQLTLPEDEPKLREMSLKDLIPGLSDIDVESLEKWNADFPGYKYHTVQAHSANMPC
FAKLKSYDDFEATAEISITSMPLMAGVIVIDRWCTVVDYNEYFVGTLLGYGRDSNKVL
RGAPVTSIIPRFTDYLEKVFASYQLGTDGNPLPIGLVIPEQAFRKVAALDPNQTNDGD
LPRLYPVTDICAVTLQGSHIHVDIQLRVVSNTHFALWVSYSRDIQGLHSEYEMPSQLS
LLDNVLEYRANLKKSGSGGGSSSNSRSSTAGSGESVSAESNTLTPMDSEDSLVTPKAA
EQSDAQLSSSETNDKNGHETDGSKPPAKKNQAVPDALSTTNRRPPVEIGARRRQKSLK
DFNIVQKLGEGAYGKVLLVQYPVEPKDVVVLKCVIKERILVDTWTRDRKLGTIPNEIK
IMNNLNALPHKNIVRLIDFFEDDLYYHIEMERHGNPGTDLFDLIELKPDMPVEECRQM
FKQVTSAVVHLHTHGIIHRDIKDENIIVDEHGDVKLIDFGSAALTRQGPFDVFVGTID
YAAPEVLSGMPYEGKPQDVWALGILLYTIMYKENPFYNVDEIMEGDLRLPYIPSESCV
DLIQKILERDIRNRPNIHTIWEHEWLQN"
gene <752760..>756962
/gene="MON2"
/locus_tag="AWJ20_4860"
/db_xref="GeneID:30037038"
mRNA <752760..>756962
/gene="MON2"
/locus_tag="AWJ20_4860"
/product="Mon2p"
/transcript_id="XM_018881960.1"
/db_xref="GeneID:30037038"
CDS 752760..756962
/gene="MON2"
/locus_tag="AWJ20_4860"
/note="Protein with a role in endocytosis and vacuole
integrity; peripheral membrane protein; interacts with and
negatively regulates Arl1p; localizes to the endosome;
member of the Sec7p family of proteins; GO_component:
GO:0005794 - Golgi apparatus [Evidence IEA]; GO_component:
GO:0000139 - Golgi membrane [Evidence IEA]; GO_component:
GO:0005829 - cytosol [Evidence TAS] [PMID 12052896];
GO_component: GO:0005768 - endosome [Evidence IDA] [PMID
12052896]; GO_component: GO:0019898 - extrinsic component
of membrane [Evidence IDA] [PMID 12052896]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005802 - trans-Golgi network [Evidence IDA] [PMID
16301316]; GO_function: GO:0005085 - guanyl-nucleotide
exchange factor activity [Evidence IGI,IPI,ISS] [PMID
12052896]; GO_process: GO:0006895 - Golgi to endosome
transport [Evidence IMP] [PMID 16301316]; GO_process:
GO:0006897 - endocytosis [Evidence IMP] [PMID 12052896];
GO_process: GO:0006623 - protein targeting to vacuole
[Evidence IMP] [PMID 12134085]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IMP,ISS] [PMID 11169758]"
/codon_start=1
/product="Mon2p"
/protein_id="XP_018736386.1"
/db_xref="GeneID:30037038"
/translation="MIFTDLCNLTEKQKPVFLKKFNHLPETFGLELLESILTNHSDIF
LKHVEFAYILRTRVVPLLLRAFSEKREFSVTVRVTRILYLLLRRQLPILKVEGEVILS
LLTHMVDEDAAPYWKRLLCMEVFQGVCSEFSLVQEIYQEYDAQEGTRRAIIKCLIAAF
GKISLERKDVIGYGPVSMPFRLPDNAATASTSGLHDENDPVDYSDVVGISLKTSVARV
PCIDLLDKSEPPIVPDTYLYYLVLVCINSLCEGLTRKVFTQSPSSSSSNADMPVKFIK
ECWHELLSCYNTFFHATIDIDLYHSLVRSVQKLTHSAGFLDISEPMDAFMSLIGSFSV
LVLEDVDGGRKSASGPSSSSSPSVAATATSMARNLLSVESIVGTLGSIREHQRSTSNA
GLPQNTSASPAPTHPPTNPGGQNTAGRSPGSPTSRNILCFRALLNLAHSLGPKLSTSW
DIVFRTIQLADSLANGSGKKRARQVQGSQVFSQLSEFSAVDTSFRRLLDSSKEFSESA
LLDMIASICRISEATLGIAKPGLSGDLFFALDLLREIGNTNMNRLVSSSDKTWPKLTD
HLLKVIDTRSVEVLARSHSADVLNDLITKASTAAVTSSTVNSDAQRKVISTLKSEIDR
VVKVQIPSEDSSIASTESDIHVSAIDTLNKVLDLCGGKFIDGWDLVFEIIDTVFTPLS
STGSSSSLSSMAVGSSGTKEIDRASRLVKSGFESMTLICNDFLIVLPEKCLINLVSTL
SKFSKQESDLNISFTSISFFWTVSDHLRNVLLEKGAAHNDFKSPISDSEKLSEVASNG
EFPLNIFALWTMSLLRLAITVGDTRAQVRNGSIQILFRILDSNGSTLPVGVWKTVQTI
VTPVVMKVRPPATNDKDVIASWTETISLILNGFSSLYSSFIGQFVKQDDFSLLWKTLI
NYLQALIDTDASHLSLPVYTALNSVLVNFSKNQNISIPAESIEEAWEFWTSQHIPESP
VDTKLTQEALTVFVQIHQPLQALSSQKDCSYEKIAKTIKILEQCTSFAHIPHLYSDRD
HMSPLQTKSLERIESIDISDVKVASLILEILSGFVLLAFSTSETKSQDPSSKRPTYIS
LAAESLQLLKTKFSIVLRADYDELCNNQTIEKIFSSLLVLMEKKFDCPLLAEKKGNKP
KQMWQTGTEIFLYILEALLDSNFDIQLSQEHITAIWDVVLKCGLAIITNKSTSSLQYE
PEYEKFDHSSYIKFISLVERGSTTSDDFWSRLLRGIFESSAIYYRGKINKEYYRIGDP
DVSVLRLVRDPFYGSTERPILKSRVNLSHLCLRELFRLSYKPPKNTALVVQQSSKFLN
WRVAITLHQYVADQPLRGRMPMPNVTRDELVKLLNLVYEYHDDKSLSQQFELVCKCIS
VSNKDKEILDLLEKCLLTIHRK"
gene complement(<757091..>760846)
/gene="CHS3"
/locus_tag="AWJ20_4861"
/db_xref="GeneID:30037039"
mRNA complement(<757091..>760846)
/gene="CHS3"
/locus_tag="AWJ20_4861"
/product="chitin synthase CHS3"
/transcript_id="XM_018881961.1"
/db_xref="GeneID:30037039"
CDS complement(757091..760846)
/gene="CHS3"
/locus_tag="AWJ20_4861"
/inference="similar to AA sequence:KEGG_Orthology:K00698"
/note="Chitin synthase III; catalyzes the transfer of
N-acetylglucosamine (GlcNAc) to chitin; required for
synthesis of the majority of cell wall chitin, the chitin
ring during bud emergence, and spore wall chitosan;
contains overlapping di-leucine and di-acidic signals that
mediate, respectively, intracellular trafficking by AP-1
and trafficking to plasma membrane by exomer complex;
requires AP-3 complex for its intracellular retention;
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 15470103]; GO_component: GO:0005935 - cellular
bud neck [Evidence IDA] [PMID 8909536]; GO_component:
GO:0045009 - chitosome [Evidence IDA] [PMID 8970154];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
9008706]; GO_component: GO:0000131 - incipient cellular
bud site [Evidence IDA] [PMID 15470103]; GO_component:
GO:0000131 - incipient cellular bud site [Evidence IDA]
[PMID 9008706]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 19053807];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005628 - prospore membrane [Evidence
IDA] [PMID 15755916]; GO_function: GO:0004100 - chitin
synthase activity [Evidence IEA]; GO_function: GO:0004100
- chitin synthase activity [Evidence IMP] [PMID 9760183];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_function: GO:0016757 - transferase activity,
transferring glycosyl groups [Evidence IEA]; GO_function:
GO:0016758 - transferase activity, transferring hexosyl
groups [Evidence IEA]; GO_process: GO:0030476 - ascospore
wall assembly [Evidence IMP] [PMID 1293886]; GO_process:
GO:0034221 - fungal-type cell wall chitin biosynthetic
process [Evidence TAS] [PMID 7565410]"
/codon_start=1
/product="chitin synthase CHS3"
/protein_id="XP_018736387.1"
/db_xref="GeneID:30037039"
/translation="MPKSSSNATIGQYEHDGIGRKRSLVRPERSRIDPDHPQYHYTQV
AAQQAGRIQVQAAPSGADPAVYEHPHTRDIPMTDLSRSQSPNEKQEYDADGGDVYVDA
KGGRNVYGLNDENGGEDNYEDNPYENGNKSYDDTNPYGVKNDMDHYGPSNLNDNDTDQ
VEPLNADDGPGSGKGGAREMSRKMSEKKAANKPLPGAKKPTSFWHAYCKIVTFWAPAP
VLKLFGMRTKDRQMAWREKMGLITVIMYVAAFVAYLTFGFTTTVCNNNLVRLRNNEVN
QGYLIINGRAYEMETFKHPEAVGIPKDTNILYPPVNAAGQDASFLFQNVNGNCRGLIT
PKADSKIPHDNKGNVAWYFPCQTFSQNGTTQPNFTFPHYNGYACHTSGKARQEYFGLK
AAAEVYFTWDDIKNSTRKLVVYNGAVLDLSLLNFIDKSNLNYPSEFTMLQKDSTIMGA
DITQLMSHGRDGQIAKCLSEVIKVGYIDTENIGCIASQVVLYVSLVFIIGLVGIKFLF
ACYFKWFISGRQGAFYMDRKQLEKHNQEIEDWSNNIYSQGPIKKAPAPPRRKDRNSKF
LKGNDKAINRQSVTMMSQIPVTSTSKLVPGGSIYGMNLGNNDASRNSFLLNNNASSDV
VGDNSFLQGDTSYRGAGGLMLEDSYSDYPPNAMPAEQQSQVYSGGPGSYDYLVPQPPV
DFQPFGFPLAHTICLVTAYSESIDGLRTTLDSIATTDYPNSHKLILIICDGIIKGSGN
DMSTPEIALSMMTDFAEPMDQVKPYSYVSVVSGAKRHNMAKVYSGFYKYDDSTVDVAK
QQRVPIMTIVKCGTPAEEGTAKPGNRGKRDSQIILMSFLQRVMFDERMTELEFEIFNG
MYKITGIAPDFYEIVLMVDADTKVFPDSITHMVAQMVKDPEVMGLCGETKIANKTESW
VSMIQVFEYFISHHQAKAFESVFGGVTCLPGCFCMYRIKAPKGDDGFWIPILANPDIV
ERYAENVVDTLHKKNLLLLGEDRFLSTLMLRTFPKRKQIFVPKAACKTIVPAEFKVLL
SQRRRWINSTVHNLMELVLVRDLCGVFCISMQFVVFVDLVSTLVLPAAITFTLYVVII
AIVRKPTPTMSLILLALILGLPGVLIVITASRLSYVLWMIIYLFSLPIWNFVLPMNAY
WKFDDFSWGDTRTIEGGDKGGHDETSGEFDSSQIIMKRWRDFQRERSRNIYPQSSGWG
EGTVSMLSSSSLNAPMSLNSHTMDHASPTTSNLGSEFVQRPEDMTQF"
gene complement(<763153..>763563)
/locus_tag="AWJ20_4862"
/db_xref="GeneID:30037040"
mRNA complement(<763153..>763563)
/locus_tag="AWJ20_4862"
/product="hypothetical protein"
/transcript_id="XM_018881962.1"
/db_xref="GeneID:30037040"
CDS complement(763153..763563)
/locus_tag="AWJ20_4862"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736388.1"
/db_xref="GeneID:30037040"
/translation="MATSTRYRRQAPTSSEIISSTTISNSEAEEVLTQYLDSITTKDS
VSHQLTRLQRSLRGLPSLLQDPEEFVDGEDQADVNMDINMDADALPNGTVKPASDGKI
SKEQRKKLKKERRKQEKKERASKPKADENDDMSE"
gene complement(<764663..>766093)
/gene="MSD1"
/locus_tag="AWJ20_4863"
/db_xref="GeneID:30037041"
mRNA complement(<764663..>766093)
/gene="MSD1"
/locus_tag="AWJ20_4863"
/product="aspartate--tRNA ligase MSD1"
/transcript_id="XM_018881963.1"
/db_xref="GeneID:30037041"
CDS complement(764663..766093)
/gene="MSD1"
/locus_tag="AWJ20_4863"
/inference="similar to AA sequence:KEGG_Orthology:K01876"
/note="Mitochondrial aspartyl-tRNA synthetase; required
for acylation of aspartyl-tRNA; yeast and bacterial
aspartyl-, asparaginyl-, and lysyl-tRNA synthetases
contain regions with high sequence similarity, suggesting
a common ancestral gene; GO_component: GO:0005737 -
cytoplasm [Evidence IEA]; GO_component: GO:0005759 -
mitochondrial matrix [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005739 - mitochondrion
[Evidence IMP,ISS] [PMID 2668951]; GO_function: GO:0005524
- ATP binding [Evidence IEA,IEA]; GO_function: GO:0004812
- aminoacyl-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0004815 - aspartate-tRNA ligase activity
[Evidence IEA]; GO_function: GO:0004815 - aspartate-tRNA
ligase activity [Evidence IMP,ISA] [PMID 2668951];
GO_function: GO:0016874 - ligase activity [Evidence
IEA,IEA]; GO_function: GO:0003676 - nucleic acid binding
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA,IEA]; GO_process: GO:0070146 -
mitochondrial aspartyl-tRNA aminoacylation [Evidence IGI]
[PMID 10710420]; GO_process: GO:0070146 - mitochondrial
aspartyl-tRNA aminoacylation [Evidence IMP] [PMID
2668951]; GO_process: GO:0006418 - tRNA aminoacylation for
protein translation [Evidence IEA]; GO_process: GO:0006412
- translation [Evidence IEA]"
/codon_start=1
/product="aspartate--tRNA ligase MSD1"
/protein_id="XP_018736389.1"
/db_xref="GeneID:30037041"
/translation="MEIETPLLFKSTPEGASEFLVPTRRKGLMYALPQSPQQYKQLLM
ASGVHGYYQIARCFRDEDLRADRQPEFTQLDLEMSFASGQDIRRVVETVVQNVWSGVL
DIPMYTLTADGKYLEKVDSDSPNAFRSLSYNSAISRFGIDKPDLRSSLEIIDLSDSAK
AIESPEYPIIEALVIRPEVNGSFTQEQIDFLSDEKSYKARIPKIALITESDLASIEQT
VERILPPSVVNITDKTRFYRELSLQTGDIVAFSTRQQLSYENPTPLGRFRQLAIEQFP
NQYLRTGADGQPLITPDDFVATWVDDFPLFNPVETTQPEVNTSNSTTSTRVDYPKFDL
TSYVSTHHPFTMVHMDDYELLSTAPLQARGQHYDLVINGVEVGGGSTRIHDTKLQKYI
FEEILNITNSKALFGHLLEAFDTGCPPHAGLAIGFDRMTAMLSRTSSIRDVIAFPKTI
TGADPLIGSPSKVKPSQLGPYHIRTM"
gene <767860..>768792
/gene="RPN11"
/locus_tag="AWJ20_4864"
/db_xref="GeneID:30037042"
mRNA <767860..>768792
/gene="RPN11"
/locus_tag="AWJ20_4864"
/product="proteasome regulatory particle lid subunit
RPN11"
/transcript_id="XM_018881964.1"
/db_xref="GeneID:30037042"
CDS 767860..768792
/gene="RPN11"
/locus_tag="AWJ20_4864"
/inference="similar to AA sequence:KEGG_Orthology:K03030"
/note="Metalloprotease subunit of 19S regulatory particle;
part of 26S proteasome lid; couples the deubiquitination
and degradation of proteasome substrates; involved,
independent of catalytic activity, in fission of
mitochondria and peroxisomes; protein abundance increases
in response to DNA replication stress; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 19773362];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 19773362]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 15210724]; GO_component: GO:0000502 -
proteasome complex [Evidence IEA]; GO_component:
GO:0008541 - proteasome regulatory particle, lid
subcomplex [Evidence IDA] [PMID 11742986]; GO_component:
GO:0008541 - proteasome regulatory particle, lid
subcomplex [Evidence IDA] [PMID 9741626]; GO_component:
GO:0034515 - proteasome storage granule [Evidence IDA]
[PMID 18504300]; GO_function: GO:0008234 - cysteine-type
peptidase activity [Evidence IEA]; GO_function: GO:0016787
- hydrolase activity [Evidence IEA]; GO_function:
GO:0008237 - metallopeptidase activity [Evidence ISS]
[PMID 12183636]; GO_function: GO:0008233 - peptidase
activity [Evidence IEA]; GO_function: GO:0004843 -
ubiquitin-specific protease activity [Evidence IMP] [PMID
12183636]; GO_function: GO:0004843 - ubiquitin-specific
protease activity [Evidence IMP] [PMID 12353037];
GO_process: GO:0000266 - mitochondrial fission [Evidence
IMP] [PMID 19773362]; GO_process: GO:0016559 - peroxisome
fission [Evidence IMP] [PMID 19773362]; GO_process:
GO:0043161 - proteasome-mediated ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 12183636];
GO_process: GO:0016579 - protein deubiquitination
[Evidence IMP] [PMID 12183636]; GO_process: GO:0016579 -
protein deubiquitination [Evidence IGI] [PMID 14581483];
GO_process: GO:0006508 - proteolysis [Evidence IEA]"
/codon_start=1
/product="proteasome regulatory particle lid subunit
RPN11"
/protein_id="XP_018736390.1"
/db_xref="GeneID:30037042"
/translation="MDHIQRLLGQGGGMGAHPGVDSPVVDNSETVYISSLALLKMLKH
GRAGVPMEVMGLMLGEFVDDFTVHVIDVFAMPQSGTGVSVEAVDDVFQTRMMDMLKQT
GRDQMVVGWYHSHPGFGCWLSSVDINTQQSFEQLNPRAVAVVVDPIQSVKGKVVIDAF
RLINPASLMMGQEPRQTTSNLGHLNKPSIQALIHGLNRHYYSININYKKTPLEETMLL
NLHKSDWSSGLALKDYTVHDHDNHDAVKQMVKLADQYTARVKEEKELTEDQLKTRYVG
KQDPKKHLEDTVNQRLEENIVSILAGNVDTASFH"
gene complement(<769241..>771364)
/gene="SSE2"
/locus_tag="AWJ20_4865"
/db_xref="GeneID:30037043"
mRNA complement(<769241..>771364)
/gene="SSE2"
/locus_tag="AWJ20_4865"
/product="adenyl-nucleotide exchange factor SSE2"
/transcript_id="XM_018881965.1"
/db_xref="GeneID:30037043"
CDS complement(769241..771364)
/gene="SSE2"
/locus_tag="AWJ20_4865"
/inference="similar to AA sequence:KEGG_Orthology:K09485"
/note="Member of the heat shock protein 70 (HSP70) family;
may be involved in protein folding; localized to the
cytoplasm; SSE2 has a paralog, SSE1, that arose from the
whole genome duplication; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_function:
GO:0005524 - ATP binding [Evidence IEA]; GO_function:
GO:0000774 - adenyl-nucleotide exchange factor activity
[Evidence IDA] [PMID 16688212]; GO_function: GO:0005516 -
calmodulin binding [Evidence IEA]; GO_function: GO:0000166
- nucleotide binding [Evidence IEA]; GO_process:
GO:0006457 - protein folding [Evidence ISS] [PMID
8406043]; GO_process: GO:0042026 - protein refolding
[Evidence IGI] [PMID 16688211]; GO_process: GO:0006950 -
response to stress [Evidence IEA]"
/codon_start=1
/product="adenyl-nucleotide exchange factor SSE2"
/protein_id="XP_018736391.1"
/db_xref="GeneID:30037043"
/translation="MSTPFGIDFGNYSSVIGVARNRGIDVVVNEVSNRATPSLVGFGV
KNRYLGEAARSNEISNLKNTVGSLKRILGRKSDDPALEIEKKFITSELVDVEGLAGVK
VRFQGEQKTFNSIQLAAMYLNKLKHITTTEIKGNVSDVVIAVPVWYSEVQRRAAADAA
IIAGLNPVRIVNDLTAAAVGYGVFKNSELPEDKPRNVAIVDFGHASYSVSIAAFKKGE
LKILATAYNRDFGGRDVDLAIANHFGKIFQEKYKIDIHSNPKAFSRVLTQAERLKKIL
SANTSAPFNIESLMNDIDVSASMTREELEEYIQPFLAQIPEPIERALKDAKLTPADLD
SIEVIGGSSRIPSVKEKLVEVFGGKSLSFTLNQDEAVARGAAFICAIHSPTVRVRPFK
FEDLNIYSVTYSWEKVEGEDVNELEVFPAGGWFPNSKVITLFRTADFDLEARYTHPET
LEKGINAWIGKWAVKGVKPSENGEAVAVKVKLRQDPSGFYTVESAYTAEERTVEEEIP
FEGELKEGEEAPEPQYKTVKKWFKKDDLTIVHTHTGLDDAAQAKLLEIESQLTSEDKL
VADTEDRKNALEEYIYDIRGKIDDLYKDFASDDEKARLREKADAAEEWLYGDGDDATK
AQYIAKYEELASIGNVIRGRYLSKIEEERQAKQAAQEAEKQRIMAEKLQAQKAAQAAA
QAEAAAKAAEEAGSQDADGDVSIEN"
gene complement(<771842..>773323)
/gene="NPL4"
/locus_tag="AWJ20_4866"
/db_xref="GeneID:30037044"
mRNA complement(<771842..>773323)
/gene="NPL4"
/locus_tag="AWJ20_4866"
/product="Npl4p"
/transcript_id="XM_018881966.1"
/db_xref="GeneID:30037044"
CDS complement(771842..773323)
/gene="NPL4"
/locus_tag="AWJ20_4866"
/inference="similar to AA sequence:KEGG_Orthology:K14015"
/note="Substrate-recruiting cofactor of the
Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the
dislocation of misfolded, polyubiquitinated ERAD
substrates that are subsequently delivered to the
proteasome for degradation; also involved in the regulated
destruction of resident ER membrane proteins, such as
HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins
(Fbp1p); role in mobilizing membrane bound transcription
factors by regulated ubiquitin/proteasome-dependent
processing (RUP); GO_component: GO:0034098 -
Cdc48p-Npl4p-Ufd1p AAA ATPase complex [Evidence IDA] [PMID
11598205]; GO_component: GO:0034098 - Cdc48p-Npl4p-Ufd1p
AAA ATPase complex [Evidence IDA] [PMID 11733065];
GO_component: GO:0036266 - Cdc48p-Npl4p-Vms1p AAA ATPase
complex [Evidence IDA] [PMID 21070972]; GO_component:
GO:0000837 - Doa10p ubiquitin ligase complex [Evidence
IDA] [PMID 16873066]; GO_component: GO:0000839 - Hrd1p
ubiquitin ligase ERAD-L complex [Evidence IDA] [PMID
16873065]; GO_component: GO:0000839 - Hrd1p ubiquitin
ligase ERAD-L complex [Evidence IDA] [PMID 16873066];
GO_component: GO:1990112 - RQC complex [Evidence IDA]
[PMID 23178123]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 22842922]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005789 - endoplasmic reticulum membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0031965 - nuclear membrane [Evidence IEA];
GO_component: GO:0042175 - nuclear outer
membrane-endoplasmic reticulum membrane network [Evidence
IDA] [PMID 11598205]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 22842922]; GO_component: GO:0048471 -
perinuclear region of cytoplasm [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0071712 - ER-associated misfolded
protein catabolic process [Evidence IMP] [PMID 11740563];
GO_process: GO:0071712 - ER-associated misfolded protein
catabolic process [Evidence IMP] [PMID 11813000];
GO_process: GO:0030433 - ER-associated ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 11739805];
GO_process: GO:0071629 - cytoplasm-associated proteasomal
ubiquitin-dependent protein catabolic process [Evidence
IMP] [PMID 18812321]; GO_process: GO:0051028 - mRNA
transport [Evidence IEA]; GO_process: GO:0072671 -
mitochondria-associated ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 21070972];
GO_process: GO:0051228 - mitotic spindle disassembly
[Evidence IMP] [PMID 14636562]; GO_process: GO:0070651 -
nonfunctional rRNA decay [Evidence IMP] [PMID 22505030];
GO_process: GO:1900182 - positive regulation of protein
localization to nucleus [Evidence IMP] [PMID 11598205];
GO_process: GO:1900182 - positive regulation of protein
localization to nucleus [Evidence IMP] [PMID 11733065];
GO_process: GO:0043161 - proteasome-mediated
ubiquitin-dependent protein catabolic process [Evidence
IMP] [PMID 20206597]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0030970 -
retrograde protein transport, ER to cytosol [Evidence IMP]
[PMID 11740563]; GO_process: GO:1990116 -
ribosome-associated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 23358411]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Npl4p"
/protein_id="XP_018736392.1"
/db_xref="GeneID:30037044"
/translation="MCRHGDKGMCDYCMPLEPFDSGYQQEKNIKHLSYHAYLRKLNAD
SNKSSIANGHGGTKGTRSGVAGSSSSYMSPLSEPFFGVAKNCPSGHPPWPQGICSKCQ
PSAITLQQQQFRMVDHVEFADSSIVNSFIESWRLTGAQRIGYLYGRYVPYEVVPLGIK
AVVEAIYEPPQSDEIDGVSLTLPWNEESLVDRVAAGLSADNSTSASGSTTGSTTGSAS
GSQSGSGQHTLGLQKIGVIYTDLLDAGKGDGSVVCKRHVDSYFLSSLEIAFAADLQNQ
HPNPSRWSDTGNFSSKFVTCVITGNSKGEIDISSYQVSTNAEAMRKADLIEPSANPSV
MRIKEPTKTRYVPEIFYKRVNEYKLTVSENAKPAFPVEYLLVTLTHGFPDSPKPLLTS
PTPFPIENRQHVGVLQDFRAIANHLNLKTGTSINNQTELSSDMSRISDFHLLVYLVGL
GILSSDEEKLLLRAAASHSPDDGYLLTQTPGWQTLLTITRESV"
gene complement(<774150..>776045)
/gene="bdc1"
/locus_tag="AWJ20_4867"
/db_xref="GeneID:30037045"
mRNA complement(<774150..>776045)
/gene="bdc1"
/locus_tag="AWJ20_4867"
/product="bromodomain containing protein 1, Bdc1"
/transcript_id="XM_018881967.1"
/db_xref="GeneID:30037045"
CDS complement(774150..776045)
/gene="bdc1"
/locus_tag="AWJ20_4867"
/codon_start=1
/product="bromodomain containing protein 1, Bdc1"
/protein_id="XP_018736393.1"
/db_xref="GeneID:30037045"
/translation="MSTSLLNLLFAQTIYGQILRDREEERLAQANGDAKSRSSSSSPA
PQSGTATGKPNQFVKLSQDLRSHPLNTNFNLTPQACQALYNALLPSNPPRPTVEMLQP
LLAKLYESYKRDMTLSIQKSEQDYQTKLKEMELIASGKWDNELLKEAERDKSQQQQQQ
QQQQQQQQLRLQKQLEIQQKQEQERKAQLQAQAQAAKPLASTDNKHDSVAESDAESES
GSEEESDQEPEASTPKPSNSVSDGKSVATSTAVDNKSKHPDEDEEAGEDEEEEEEEEE
EGEEENEKEGADVEEGEEEELEEEDTTLKSSLKGPSDEVDEGTVKQETQEKADKPDAS
VKTETGDNNEGEEKEDEDEDVEMADLEADADADAEESEVPAKDNVKKEHATDKEDTQE
VEDEEEEVEEGDEEEEPETVEQEAKSAPPQKPVEDDEEAEEEAEEAEDADEEEAEEAE
DETEVPDETRRTTRQSRRNKQEPATTTTSSSKRKRTPSPSPARPSRRFQTLVNPLISD
IMSNKSASFFSHPVNPNDAPAYYDLIYSPTDLRTIKSMVKEGKIRDTAELERELEKMF
ANAVMYNGWDSDISMWAREMQHDTETMLALFRSAERRGRARSNSATPSGADDEPSNTN
SAKRRKK"
gene <777130..>777489
/gene="SEC66"
/locus_tag="AWJ20_4868"
/db_xref="GeneID:30037046"
mRNA <777130..>777489
/gene="SEC66"
/locus_tag="AWJ20_4868"
/product="Sec63 complex subunit SEC66"
/transcript_id="XM_018881968.1"
/db_xref="GeneID:30037046"
CDS 777130..777489
/gene="SEC66"
/locus_tag="AWJ20_4868"
/inference="similar to AA sequence:KEGG_Orthology:K12273"
/codon_start=1
/product="Sec63 complex subunit SEC66"
/protein_id="XP_018736394.1"
/db_xref="GeneID:30037046"
/translation="MKAAILKWAAEDIRQMIRMNDSKQYLTVLHQRGSVGDDIWKRFT
MSEKFLQLELEDIRREAEAIHPNWTQQLFQTASEIAQNEGLRKRINEFPAQQQEYRQA
FEALRENSIKELTSEKN"
gene complement(<780196..>784647)
/gene="SMY2"
/locus_tag="AWJ20_4869"
/db_xref="GeneID:30037047"
mRNA complement(<780196..>784647)
/gene="SMY2"
/locus_tag="AWJ20_4869"
/product="Smy2p"
/transcript_id="XM_018881969.1"
/db_xref="GeneID:30037047"
CDS complement(780196..784647)
/gene="SMY2"
/locus_tag="AWJ20_4869"
/note="GYF domain protein; involved in COPII vesicle
formation; interacts with the Sec23p/Sec24p subcomplex;
overexpression suppresses the temperature sensitivity of a
myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog,
SYH1, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0000932 - cytoplasmic mRNA
processing body [Evidence IDA] [PMID 17804396];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IDA] [PMID 17973654]; GO_component: GO:0019898 -
extrinsic component of membrane [Evidence IDA] [PMID
17973654]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006888 - ER to Golgi
vesicle-mediated transport [Evidence IGI,IPI] [PMID
17973654]"
/codon_start=1
/product="Smy2p"
/protein_id="XP_018736395.1"
/db_xref="GeneID:30037047"
/translation="MSKSSALSLGPEWYVSSQCCLAPLVARSSSGGGLPPAAGAPPQT
LVAPASQELVGFVDGIDSSEARGAGGSGAQPQPPEARLFVELTGIRTRLKSGSSKVLK
DDKTANGNNNSPNGPEKVKLPWSGIAAGAASSANSIASGSSNSSNGGGVAAAGPGLAG
ASSASGPSFAAAASALKRKTSGTNVSPSVTSSAGSATASGSAGAASGAGAGRNSGASG
ISDINNSAAGGGVFAISAAAGGSGLFGMRKSSSGDDSDATSQSSLSGKRVGSTSTTTA
TTTNTHANSGLSTGNISSPVTGLGAITGSGLAGSVPYKVGGTRVAVSLEDSVQGVTAG
ASDSAAGAAGASGSGSSSNGPKSEPVVLRANAGAKKYSSKEMLAIWDRIKNSKEFRES
KRIAVTELTENSDPASAPASGSGSANTITTNTSFNTNIGNNGQQRGYHNRYHHGNNNT
NNNNNNGGGVGGGSKLFTPFGRYKNPNDLYYSNMTNMPVNYDTPPKQRPYRDGDDRNA
HVHGSSHGTGHGSSQGSGTGTGTGTTVDDIGRKSGGVSLTSVTGAGGAATTETGLRSG
GTGNGSRLMDTINSASSKVADDAFFSSLVSSGARSDSGGQVAQNQPQAPAPTTSIASN
ILPASIASDSWSPFGNFSITPSSARNDLSSHPSDTALSRIGSGTNLLGISKPGSTPTA
SAVAGGSTASDFFSSPIKRVGTPLSNAAMAATPPPGIPSPQPNPPLILPENVQWVYKD
PSGVEQGPFSGKLMHDWYTGNWLKEDLLIKRIGETDYITLHDFKLKIGNFTEPFRVPL
PPVLPPSLPPPFGQQQQQPSPFGHGIPYFDDLQRDVFRQHHHQAQQPLQLPNQHQQFG
GAAPVIPSAWGASGSPVVSPGPMSPISPWGQPIQPVHQPATHSQSNFFQDQPLHSQLA
PVLSHSSQDTSAAAATAASVANSAVGSPVQEPSSGPLGQSLWKGLDSVQRDLSSSGSV
ADIINNAENLSLNDDIKAQTQAEPVSEETSTGASSTKSEDNSESKRELTKAEIKKAKK
KAKAELQSAAAAAVAAAAAERAEKANNKQEHSEPTASTDDDSADQTTKTATSSVESTP
VTSGSNSTHAATSTVASTGSTGHGHTLTLSTTTSPTATAAPALAPWAKKDTKPKALSL
KEIQELEASERAKQTAKQQELVQQQLLAARLAGGVSSSSTSGPILPSGAKWASSMNSA
ASPKKTLAEIQREEELAAAAAAKKKAQALAQAQAQTISSPIAGTTASTGVPTGASGAT
PTISPIAASLPFGKKYAEIVTPGAPSSVRPGVSSATSSSLATGLSSTGAPAWTTVGPG
GRKVSSPATTGVAASIAAGGSSPLKRAISSTTLRPAAAPVPTAPRVLTASDEFLQWCK
GALQDLNAGINQTELLSILLSLPATPDSKEIIAETIYSNSSTMDGRRFADEFLKKRNV
ADKEKSGETWSDVLQRNATAPPKPVENDGWNPAFKVVKKKNKRADQF"
gene <785371..>787437
/gene="SAD1"
/locus_tag="AWJ20_4870"
/db_xref="GeneID:30037049"
mRNA <785371..>787437
/gene="SAD1"
/locus_tag="AWJ20_4870"
/product="Sad1p"
/transcript_id="XM_018881971.1"
/db_xref="GeneID:30037049"
CDS 785371..787437
/gene="SAD1"
/locus_tag="AWJ20_4870"
/inference="similar to AA sequence:KEGG_Orthology:K12847"
/note="Conserved zinc-finger domain protein involved in
pre-mRNA splicing; critical for splicing of nearly all
intron-containing genes; required for assembly of U4 snRNA
into the U4/U6 particle; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 10022888]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0005681 - spliceosomal complex [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0008270
- zinc ion binding [Evidence IEA]; GO_process: GO:0008380
- RNA splicing [Evidence IEA]; GO_process: GO:0006397 -
mRNA processing [Evidence IEA]; GO_process: GO:0000398 -
mRNA splicing, via spliceosome [Evidence IDA] [PMID
10022888]; GO_process: GO:0000245 - spliceosomal complex
assembly [Evidence IDA] [PMID 24681967]; GO_process:
GO:0006511 - ubiquitin-dependent protein catabolic process
[Evidence IEA]"
/codon_start=1
/product="Sad1p"
/protein_id="XP_018736396.1"
/db_xref="GeneID:30037049"
/translation="MGNGDETRAADEAALVDTEISAVESVEQNGSEIQLEHEHEHQHE
HEHEHEINNSFEKAGSVKEIENGDTTEMRTRKRRLNEEKNDDDENETIHTNGSVPGTS
TPDSSEARGAVGSGAKPQPPEVRRHPSNSQSKIEEEQKEEEEEEEGVWPVRPVRQVQE
PSSSMLYLDTVDRSRLDFDFEKICSVSLSVVNVYACLCCGKYFQGRGKSSYAFVHSVD
VDHHVFINLQTLKIYVLPEGYEVKTTALDDIKYVIDPWYTADEIKSLDTVALNSSFDL
THKQYYPGYIGINNIKANDYANVVIQILSHVSPVRDFFLTLGQGRATGSSQSNGSSAS
ISMANSMANSTNGTSQAGSSELASRLSTLIRKIWNPRAFRAHVSPHELLQSVSSASKK
QFTSTQQSDPFLFFTWLLNRLTAELTVGPTNTRFSRDSSPGTPTAAITTAVSTTTTKD
QQPVKKKLKPTSTRSSKPIKPRGIIQKTFQGTLLVEQQQLPRTLPSGASADTFLPNEP
IKTTETPFLCLTLDLPPIPLFKESTQDGAPVIPQVTLKSLLNKYNGTTVTTDEITKTM
KKYKLTRVPEFLVLRIKRITRNLLGDADERNPTVVSFSPTNLDMAPYLTSSSTTSPSS
TRYNLIANVVCEWDGNTPRWKIQLLDKSRDRWLQIENLLVEPIQRELLFLSESHLQIW
QRQSPL"
gene complement(<788160..>788480)
/gene="UMP1"
/locus_tag="AWJ20_4871"
/db_xref="GeneID:30037050"
mRNA complement(<788160..>788480)
/gene="UMP1"
/locus_tag="AWJ20_4871"
/product="Ump1p"
/transcript_id="XM_018881972.1"
/db_xref="GeneID:30037050"
CDS complement(788160..788480)
/gene="UMP1"
/locus_tag="AWJ20_4871"
/inference="similar to AA sequence:KEGG_Orthology:K11599"
/note="Chaperone required for correct maturation of the
20S proteasome; short-lived chaperone; may inhibit
premature dimerization of proteasome half-mers; degraded
by proteasome upon completion of its assembly;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
11922673]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 11922673]; GO_component: GO:0000502 -
proteasome complex [Evidence IEA]; GO_function: GO:0003674
- molecular_function [Evidence ND]; GO_process: GO:0006974
- cellular response to DNA damage stimulus [Evidence IDA]
[PMID 10975253]; GO_process: GO:0043248 - proteasome
assembly [Evidence IGI] [PMID 17431397]; GO_process:
GO:0043248 - proteasome assembly [Evidence IMP] [PMID
9491890]; GO_process: GO:0006511 - ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 9491890]"
/codon_start=1
/product="Ump1p"
/protein_id="XP_018736397.1"
/db_xref="GeneID:30037050"
/translation="MRSIAAEMNGNHPLQSRLEKWNETQLEFKLDGYRRTYGAGEPIR
RAMELQIVKDTSVLPKIVTGPSRPLHLDILEGRDDAVDWDEVYTGPESTLDFHSELEK
RMNV"
gene <789365..>790984
/gene="PTC5"
/locus_tag="AWJ20_4872"
/db_xref="GeneID:30037051"
mRNA <789365..>790984
/gene="PTC5"
/locus_tag="AWJ20_4872"
/product="type 2C protein phosphatase PTC5"
/transcript_id="XM_018881973.1"
/db_xref="GeneID:30037051"
CDS 789365..790984
/gene="PTC5"
/locus_tag="AWJ20_4872"
/inference="similar to AA sequence:KEGG_Orthology:K01102"
/note="Mitochondrial type 2C protein phosphatase (PP2C);
involved in regulation of pyruvate dehydrogenase activity
by dephosphorylating the serine 133 of the Pda1p subunit;
localizes to the intermembrane space and is imported via
the presequence pathway and processed by the inner
membrane protease (Imp1p-Imp2p); acts in concert with
kinases Pkp1p and Pkp2p and phosphatase Ptc6p;
GO_component: GO:0005758 - mitochondrial intermembrane
space [Evidence IEA]; GO_component: GO:0005758 -
mitochondrial intermembrane space [Evidence IDA] [PMID
22984289]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16643908];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0004741 - [pyruvate
dehydrogenase (lipoamide)] phosphatase activity [Evidence
IEA]; GO_function: GO:0004741 - [pyruvate dehydrogenase
(lipoamide)] phosphatase activity [Evidence ISS] [PMID
16643908]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0046872 - metal
ion binding [Evidence IEA]; GO_function: GO:0004721 -
phosphoprotein phosphatase activity [Evidence IEA];
GO_function: GO:0004722 - protein serine/threonine
phosphatase activity [Evidence IEA]; GO_function:
GO:0004722 - protein serine/threonine phosphatase activity
[Evidence IDA,ISS] [PMID 10580002]; GO_process: GO:0043085
- positive regulation of catalytic activity [Evidence IMP]
[PMID 18180296]; GO_process: GO:0006470 - protein
dephosphorylation [Evidence IEA]; GO_process: GO:0006470 -
protein dephosphorylation [Evidence IDA] [PMID 10580002];
GO_process: GO:0050790 - regulation of catalytic activity
[Evidence IMP] [PMID 16643908]"
/codon_start=1
/product="type 2C protein phosphatase PTC5"
/protein_id="XP_018736398.1"
/db_xref="GeneID:30037051"
/translation="MSNPRVANDSPGPGGPGITGPSQAAGPVRLASNSAKQSISMLTP
AQVSSRLRENEESYLVDRGKGVLRYDVAQLPSNNPIEDDRSERVVQVPLIVEPSGSTS
SGSNSNSGSGNGVSEKSEVASGGETGENGETMVSSDWMFWGVYDGHSGWTTSAFLRDH
LVAYVLNELDKVYEKSSPNSMYRLVPSPEVIDEAIQQGFLALDDEIVNKRVARLLESS
GSSKASATELIAPALSGSCGLLAFYDTFSKNLRVAVTGDSRAVLGSRDSYGNWTATAL
STDQTGSNIDEANRVRSEHPGEENTAIRNGRVLGSLEPTRAFGDARYKWARDIQHKIA
QRFFGRRIPSELKTPPYVTAKPVVTTTKVNPENGDFLVMGSDGVFEMLSNEEVVSLVV
QWLKAKRPEYLETTLKSSQSDGSGLFSKLFGSGKRSDRDDGALVEDISSNKEAQKQPI
RRRNGVPVRFTVQDDNAATHIIRNALGGADQDQVSMLVSIPSPLARNYRDDLTVTVVF
FGHDSTPSNDAGSIKVNQAGTRNGIRARSRL"
gene complement(<791263..>792141)
/locus_tag="AWJ20_4873"
/db_xref="GeneID:30037052"
mRNA complement(<791263..>792141)
/locus_tag="AWJ20_4873"
/product="glucose-6-phosphate 1-epimerase"
/transcript_id="XM_018881974.1"
/db_xref="GeneID:30037052"
CDS complement(791263..792141)
/locus_tag="AWJ20_4873"
/inference="similar to AA sequence:KEGG_Orthology:K01792"
/note="Glucose-6-phosphate 1-epimerase (hexose-6-phosphate
mutarotase); likely involved in carbohydrate metabolism;
GFP-fusion protein localizes to both the nucleus and
cytoplasm and is induced in response to the DNA-damaging
agent MMS; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0030246 -
carbohydrate binding [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0047938 - glucose-6-phosphate 1-epimerase
activity [Evidence IEA]; GO_function: GO:0047938 -
glucose-6-phosphate 1-epimerase activity [Evidence IDA]
[PMID 16857670]; GO_function: GO:0016853 - isomerase
activity [Evidence IEA,IEA]; GO_process: GO:0005975 -
carbohydrate metabolic process [Evidence IEA]; GO_process:
GO:0044262 - cellular carbohydrate metabolic process
[Evidence IC] [PMID 16857670]"
/codon_start=1
/product="glucose-6-phosphate 1-epimerase"
/protein_id="XP_018736399.1"
/db_xref="GeneID:30037052"
/translation="MASYIKETETEIVLTHPRDSSTSVRILKYGATILSWKVKGQEQL
WLSEKAILDGSKAVRGGIPLVFPVFGKSEGHETSQLPQHGFARNSVWEFLGQVESDPL
TVQFGLGPENISETARCQWPHDFTLIFTVSLPSEDSLSTSLEVENTDKQPWEFNVLFH
TYFRIPDITQTHVEGLTGTQVTDKVSKTNYAGNEAKITIAGEVDRVYSSPSTPDSVAI
VSGSKTLFNVQNLQNLKDVVVWNPWTEKANGLADFSPKDGFKNMICVETGSVSKYQTV
APGAKWSASQTLKSHL"
gene <793841..>795592
/gene="SIT1"
/locus_tag="AWJ20_4874"
/db_xref="GeneID:30037053"
mRNA <793841..>795592
/gene="SIT1"
/locus_tag="AWJ20_4874"
/product="Sit1p"
/transcript_id="XM_018881975.1"
/db_xref="GeneID:30037053"
CDS 793841..795592
/gene="SIT1"
/locus_tag="AWJ20_4874"
/inference="similar to AA sequence:KEGG_Orthology:K08197"
/note="Ferrioxamine B transporter; member of the ARN
family of transporters that specifically recognize
siderophore-iron chelates; transcription is induced during
iron deprivation and diauxic shift; potentially
phosphorylated by Cdc28p; GO_component: GO:0016023 -
cytoplasmic membrane-bounded vesicle [Evidence IDA] [PMID
10748025]; GO_component: GO:0005768 - endosome [Evidence
IEA]; GO_component: GO:0005768 - endosome [Evidence IDA]
[PMID 19469713]; GO_component: GO:0010008 - endosome
membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_function: GO:0015344 -
siderophore uptake transmembrane transporter activity
[Evidence IMP,ISS] [PMID 9884238]; GO_process: GO:0006879
- cellular iron ion homeostasis [Evidence IDA,IMP] [PMID
9884238]; GO_process: GO:0006811 - ion transport [Evidence
IEA]; GO_process: GO:0055072 - iron ion homeostasis
[Evidence IEA]; GO_process: GO:0015891 - siderophore
transport [Evidence IMP] [PMID 9884238]; GO_process:
GO:0055085 - transmembrane transport [Evidence IMP] [PMID
9884238]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Sit1p"
/protein_id="XP_018736400.1"
/db_xref="GeneID:30037053"
/translation="MVSDSVEEKSRDGPEGYQPSYEGFDQQAEKEKSIGVRKVEIYSS
QYKLVGKIFVFFSVFLVSYAYGLDSNIRYVYQATATSSYAEHSLLSTVNVIKAVIGAA
GQPVYARLSDVFGRLELFIAAILFYVVGTIIESQAYDVQRFAGGAILFQVGYTGIVLL
MELIVADFSFLNWRLVASFVPALPFIINTWISGNITGDMGLRWSWGIGMWAIIFPVAS
IPLLICLIHMRYRAHKSGQMDAIKAEETDFQRLGFWGFMKHLFWQLDVVGIILLIAVF
ALILVPFTIAGGVEEEWKKAKIIAPLVIGFCCIPVFAVWEFKYAPIPLVPMKHLRNRT
VYSAVCIAVLINFIWYMQGDYMYTVLVVAVNQSTTSANRITSLYSFVSVITGTILGLI
VARLRYLKGFIVFGTCMWLLSMGLLIHYRGGTDSKSGIIGSMCVLGFGAGLFTYPVQV
MIQASVKHEHMAIITSLYLASYNIGSALGGSVSGAIWTQILPKELSKRLSDPALVTAA
YGSPFTFIISYPWGTPERMAVVEAYKHVQKILLIVGTCLCVPLIFFALIIDNYKLESV
QSLPGAEKDGESESLSS"
gene <799253..>800890
/locus_tag="AWJ20_4875"
/db_xref="GeneID:30037054"
mRNA <799253..>800890
/locus_tag="AWJ20_4875"
/product="hypothetical protein"
/transcript_id="XM_018881976.1"
/db_xref="GeneID:30037054"
CDS 799253..800890
/locus_tag="AWJ20_4875"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736401.1"
/db_xref="GeneID:30037054"
/translation="MIVIGEENAVGWSTKVAIVGSGPSALSLSFILNGNIPYYDPVPY
GPHPDESLHYCLLNYTAGNDLGKSLLDAVADEHIISYMESSYESFFSDQTLPVNLLID
ALSASDESSFMNCHSKECRIKWVHESAKYVPHIVIGSSPRPGGQWGSYEFDSKDQLSL
SYAEMLSLPGYSFSQYYMDTYGTTPADFYRPPRHQVADYYAMYPGKVGISENIVSNTL
VTCVDRASHTGSFVISMRQCEGAVHKQCLLAADKVVLATGVYESPLESLKSASPKGIN
ENNGTNTTDLTDVNSNGEYGRHDKTFYNLTNALVHNNSTSNSNSNSNSFDKHHFSFTI
VPPLSTPPSEGARASQNKTCLIIGTGVSAAEAVNNSIALGYSVIHIYKWYDEKGSPCV
FRRYPKELYPDYCKVFRMMKQRKVAGLYEGLPNAQILDISPDGMVEIKLEDGSTVCRT
VSSVRACTGRTGSLSYLCNPVLDRSIESTIRTMPITKKTLRDKYIDKNDQSLCVGENV
YAIGSLCGDTVVRFMLGASFSVAGELVDSSSAKSVGS"
gene complement(<800776..>801924)
/locus_tag="AWJ20_4876"
/db_xref="GeneID:30037055"
mRNA complement(<800776..>801924)
/locus_tag="AWJ20_4876"
/product="hypothetical protein"
/transcript_id="XM_018881977.1"
/db_xref="GeneID:30037055"
CDS complement(800776..801924)
/locus_tag="AWJ20_4876"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736402.1"
/db_xref="GeneID:30037055"
/translation="MASAKGKGVPSQFKDLRNQLKKDENSAESLMELVLRHGPVKMLV
NQDPGILHVLCTERPVLSVDRALEILSDLVSVRGDSEFEEVSLERIGSAGSDSEKLVG
VSWVILRNAVSDKRFTEAFKVVDLVKRYEQQQRQRRRGIVGSSKVIINYISTVLGGKR
SGASASASASASASSESAKASKSKSISASASAVVAAATLVPSLALYILSGPAALLVPI
VYSSIALSYFVRWHLLPYPFYLPNLFGSISGSLLTWAPSVGPIYRAQRQLELDMVNRI
VVGYSELVDINTSNYHVHHSREMPPVELENLINSQLLSRSMRLKPTHDEAMYQEYWQS
AGQNLEWVEPDQDPTDLALDESTSSPATEKLAPSINRTTVSPHKLPIA"
gene complement(<802480..>802965)
/gene="VPS21"
/locus_tag="AWJ20_4877"
/db_xref="GeneID:30037056"
mRNA complement(<802480..>802965)
/gene="VPS21"
/locus_tag="AWJ20_4877"
/product="Rab family GTPase VPS21"
/transcript_id="XM_018881978.1"
/db_xref="GeneID:30037056"
CDS complement(802480..802965)
/gene="VPS21"
/locus_tag="AWJ20_4877"
/inference="similar to AA sequence:KEGG_Orthology:K07889"
/note="Endosomal Rab family GTPase; required for endocytic
transport and sorting of vacuolar hydrolases; required for
endosomal localization of the CORVET complex; required
with YPT52 for MVB biogenesis and sorting; involved in
autophagy and ionic stress tolerance; geranylgeranylation
required for membrane association; protein abundance
increases in response to DNA replication stress; mammalian
Rab5 homolog; VPS21 has a paralog, YPT53, that arose from
the whole genome duplication; GO_component: GO:0005768 -
endosome [Evidence IEA]; GO_component: GO:0010008 -
endosome membrane [Evidence IEA]; GO_component: GO:0005770
- late endosome [Evidence IMP] [PMID 11872141];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0031966 - mitochondrial membrane
[Evidence IEA]; GO_component: GO:0005741 - mitochondrial
outer membrane [Evidence IDA] [PMID 16407407];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0005525 -
GTP binding [Evidence IEA,IEA]; GO_function: GO:0003924 -
GTPase activity [Evidence IDA] [PMID 11210571];
GO_function: GO:0003924 - GTPase activity [Evidence IMP]
[PMID 8163546]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006895 - Golgi to
endosome transport [Evidence IGI,IMP] [PMID 22748138];
GO_process: GO:0006895 - Golgi to endosome transport
[Evidence IGI,IMP] [PMID 23612966]; GO_process: GO:0006897
- endocytosis [Evidence IMP] [PMID 8163546]; GO_process:
GO:0032511 - late endosome to vacuole transport via
multivesicular body sorting pathway [Evidence IGI] [PMID
22748138]; GO_process: GO:0036258 - multivesicular body
assembly [Evidence IGI] [PMID 22748138]; GO_process:
GO:0036010 - protein localization to endosome [Evidence
IGI] [PMID 23264632]; GO_process: GO:0006623 - protein
targeting to vacuole [Evidence IMP] [PMID 8163546];
GO_process: GO:0015031 - protein transport [Evidence
IEA,IEA]; GO_process: GO:0007264 - small GTPase mediated
signal transduction [Evidence IEA]; GO_process: GO:0006810
- transport [Evidence IEA]; GO_process: GO:0000011 -
vacuole inheritance [Evidence IMP] [PMID 1493335]"
/codon_start=1
/product="Rab family GTPase VPS21"
/protein_id="XP_018736403.1"
/db_xref="GeneID:30037056"
/translation="MYYRNAQSALVVYDVTKPASFVKARHWVNELKAQASPGIVIALV
GNKVDLVESDEDGSSGDAANGDGSAGSEGGSESARKVAIEEGKALAENEDLLFFETSA
KSGLNVRQVFLAIANKIPQESGRRVATAVNGARTGADAGGRVDLNTPATASSSPSACN
C"
gene complement(<806682..>808730)
/locus_tag="AWJ20_4878"
/db_xref="GeneID:30037057"
mRNA complement(<806682..>808730)
/locus_tag="AWJ20_4878"
/product="hypothetical protein"
/transcript_id="XM_018881979.1"
/db_xref="GeneID:30037057"
CDS complement(806682..808730)
/locus_tag="AWJ20_4878"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736404.1"
/db_xref="GeneID:30037057"
/translation="MSTAVDNGAQGSAAQNEIQSGSGVSVGAGGAGGADGTGGTGGKG
SIGETKGGGVQNEQIGLANGSGAGAGIIKSTSGSPALSGPLAPRTTFDLEPNPFEQSF
ASNATSTGSKSVSPANISGTAGVVVGAGGAGGAGIGIGAGIGGISGISGAAGGANGGA
NGGLGPLAPSIIGTTGTGQQIVATPGGRRHILPPVTALASPSALLSNPGSPGSSAWAN
SLKSGPLSPAMLQAPQSTEGTTPGGSVSVTGGLRLGAVPTLTSMIRTGLTPGGSGSMF
PTTPGPTTAAILGLTGPQPDGGLLAGHLLPNGAPVSQGQSPAQTQQQQQQQQQQQQGH
QAHLHGQNVANQNHGQTYPQRGIPANVAGAATGPTGLHQDQANAAANGLFMLSQTNGG
PGGPPGGHGNANSRGKGGADSSNARDGSSGGKSGSAGAAKTSNNNEKKGVKRSRKPDP
KDNAKERDTKAAKTKSKNTSTTARDTGIKNEQSDDNLDSDQDDDNDDDQNDTSNNSNG
SSNNKKSKQSVDEKRKSFLERNRVAALKCRQRKKQWLSDLQNKVEYLGTENEKLASQL
AMMADQVNGLKTVLLAHRDCGVPLPPNLLNMLMNERVMDPNMGDFRTTAAASAANGAQ
GTTQPPPNLGLLPTGLNTGIQNVPGDVALAQAHAQAQAQAHAQQQGLPQVNYRTVWPL
"
gene <811385..>813019
/gene="ICL1"
/locus_tag="AWJ20_4879"
/db_xref="GeneID:30037058"
mRNA <811385..>813019
/gene="ICL1"
/locus_tag="AWJ20_4879"
/product="isocitrate lyase 1"
/transcript_id="XM_018881980.1"
/db_xref="GeneID:30037058"
CDS 811385..813019
/gene="ICL1"
/locus_tag="AWJ20_4879"
/inference="similar to AA sequence:KEGG_Orthology:K01637"
/note="Isocitrate lyase; catalyzes the formation of
succinate and glyoxylate from isocitrate, a key reaction
of the glyoxylate cycle; expression of ICL1 is induced by
growth on ethanol and repressed by growth on glucose;
GO_component: GO:0005575 - cellular_component [Evidence
ND]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0004451 - isocitrate lyase
activity [Evidence IEA,IEA]; GO_function: GO:0004451 -
isocitrate lyase activity [Evidence IMP,ISS] [PMID
1551398]; GO_function: GO:0016829 - lyase activity
[Evidence IEA]; GO_process: GO:0019752 - carboxylic acid
metabolic process [Evidence IEA]; GO_process: GO:0006097 -
glyoxylate cycle [Evidence IEA,IEA]; GO_process:
GO:0006097 - glyoxylate cycle [Evidence TAS] [PMID
11092862]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0006099 - tricarboxylic
acid cycle [Evidence IEA]"
/codon_start=1
/product="isocitrate lyase 1"
/protein_id="XP_018736405.1"
/db_xref="GeneID:30037058"
/translation="MSVNIKAEEQAFQDEVAQLNAWWKEPRWAQTKRVYPAEEIARKR
GTLLPKYSSSTQADKLYKLLQEHDKNKTASFTYGALDPVQVTQMAKFLDTIYVSGWQS
SSTASTSNEPSPDLADYPMDTVPNKVEHLWFAQQFHDRKQREDRLSVPLSQREKLPYV
DYLRPIIADADTGHGGLTAIVKLTKMFIERGAAGIHIEDQAPGTKKCGHMAGKVLVPI
QEHINRLIAIRASADIFGSNLLAIARTDSEAATLLTSTIDYRDHYFIVGSTNPNIPPL
VDVMVEAEQRGVVGAELQKVEDNWIAQAKLKLFHEAVVDEINAGSYSNKKELTDKFLS
AVGPLTNQSNNQARQLAKKLLGKDVYFDWEKPRVREGYYRYRGGTQCAVNRARAYAPY
CDLIWMESKLPDYAQAKEFADGVKAAYPNKWLAYNLSPSFNWTTAMSPSDQRTYIDRL
AKLGYVWQFITLAGLHTNALISHNFAKRYSEIGMQAYGGDIQQPELDSGVDVVKHQKW
SGAEYVDNILKMITGGVTSTAAMGAGVTEDQFKSKA"
gene <816619..>818925
/gene="RGD1"
/locus_tag="AWJ20_4880"
/db_xref="GeneID:30037060"
mRNA <816619..>818925
/gene="RGD1"
/locus_tag="AWJ20_4880"
/product="Rgd1p"
/transcript_id="XM_018881982.1"
/db_xref="GeneID:30037060"
CDS 816619..818925
/gene="RGD1"
/locus_tag="AWJ20_4880"
/note="GTPase-activating protein (RhoGAP) for Rho3p and
Rho4p; possibly involved in control of actin cytoskeleton
organization; GO_component: GO:0030479 - actin cortical
patch [Evidence IDA] [PMID 10931290]; GO_component:
GO:0005933 - cellular bud [Evidence IDA] [PMID 10931290];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 19053807]; GO_component: GO:0005628 - prospore
membrane [Evidence IDA] [PMID 24390141]; GO_function:
GO:0005096 - GTPase activator activity [Evidence IEA];
GO_function: GO:0005100 - Rho GTPase activator activity
[Evidence IDA,IGI] [PMID 10526184]; GO_function:
GO:0080025 - phosphatidylinositol-3,5-bisphosphate binding
[Evidence IDA,IMP] [PMID 18845541]; GO_function:
GO:0032266 - phosphatidylinositol-3-phosphate binding
[Evidence IDA,IMP] [PMID 18845541]; GO_function:
GO:0005546 - phosphatidylinositol-4,5-bisphosphate binding
[Evidence IDA] [PMID 21215255]; GO_function: GO:0070273 -
phosphatidylinositol-4-phosphate binding [Evidence
IDA,IMP] [PMID 18845541]; GO_function: GO:0010314 -
phosphatidylinositol-5-phosphate binding [Evidence
IDA,IMP] [PMID 18845541]; GO_process: GO:0007231 -
osmosensory signaling pathway [Evidence IGI] [PMID
10590461]; GO_process: GO:0043547 - positive regulation of
GTPase activity [Evidence IEA]; GO_process: GO:0001101 -
response to acid [Evidence IMP] [PMID 16087742];
GO_process: GO:0007165 - signal transduction [Evidence
IEA]"
/codon_start=1
/product="Rgd1p"
/protein_id="XP_018736406.1"
/db_xref="GeneID:30037060"
/translation="MDSSNTPAANNGRSASNGSRLSNNTTTSSDEGDSNVSNNAPSTA
PTGSALTGPPITGPGIRKTSTVSTIGGGTGGSTSAGPASSIAPTTTTFNSSDTATTYS
LPQPASSSYLLEDARLREILMSDVGTEALLKRLKQSIHAAKELSSFLKKSSNLENEHM
GQVRKLASSAISEFAKPEYRQGTFAGQLNQLIKVNERSCDAARSFTTALVTMQKELAN
LSATVDKSRKSIKESALRHERQLLEAEQQAEKAKSKYDSLAEEFDKVRTGDPTKNKFG
FMHSKGAQHEEELHKKVQTAESDYKQKVENAQRLRSEQINKNRPAAIKQLKDLILECD
SAMSFQLQKYANLHEILALNRGFIVAPLKPNGAANGNLTMKEMAAKIDNELDLYNYVI
GVPKTVSRLNRPLVQFQRHSTLAGAYPTGSIGNTTSSVPQSFQNNTTSPPPPISKAPG
AGIGGTVGAGIGAGVGAGAGLAAGLASGPNGPGSAGAGAGAPTAPAALNSHTAIGPSP
VLPPVATSEPFSVYPGNDTISPSSTIETQAVSTTASNFDTSAGPVPTVGQPGSGYPPG
TVHSRPSFGTPLDVLLDYEEATVPRVVFQCIQAVDNFGLEVEGIYRTSGNNNQVQEIK
RLFDLDAASVDLLHPTHGLNDIHSVAGALKLYFRELPDPLLTGQFHDEFVAAAKLPAG
DERRDGVHRIVNELPDPNYTTLRHLIFHLYRVQTREASNRMSISNLAIVWGPTLMSSS
LNNPEDMALEASVIETILVNAYTIFDAD"
gene complement(<819143..>821929)
/gene="CUL3"
/locus_tag="AWJ20_4881"
/db_xref="GeneID:30037061"
mRNA complement(<819143..>821929)
/gene="CUL3"
/locus_tag="AWJ20_4881"
/product="cullin CUL3"
/transcript_id="XM_018881983.1"
/db_xref="GeneID:30037061"
CDS complement(819143..821929)
/gene="CUL3"
/locus_tag="AWJ20_4881"
/inference="similar to AA sequence:KEGG_Orthology:K10609"
/note="Ubiquitin-protein ligase; forms a complex with
Elc1p that polyubiquitylates monoubiquitylated RNA
polymerase II to trigger its proteolysis; cullin family
member with similarity to Cdc53p and human CUL3;
GO_component: GO:0031463 - Cul3-RING ubiquitin ligase
complex [Evidence IDA] [PMID 16675952]; GO_component:
GO:0031463 - Cul3-RING ubiquitin ligase complex [Evidence
IMP] [PMID 17296727]; GO_component: GO:0031461 -
cullin-RING ubiquitin ligase complex [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0031625 - ubiquitin
protein ligase binding [Evidence IEA]; GO_function:
GO:0004842 - ubiquitin-protein transferase activity
[Evidence IDA] [PMID 12676951]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence IDA]
[PMID 16675952]; GO_process: GO:0016567 - protein
ubiquitination [Evidence IEA]; GO_process: GO:0042787 -
protein ubiquitination involved in ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 17296727];
GO_process: GO:0006511 - ubiquitin-dependent protein
catabolic process [Evidence IEA]; GO_process: GO:0006511 -
ubiquitin-dependent protein catabolic process [Evidence
IC] [PMID 12676951]; GO_process: GO:0006511 -
ubiquitin-dependent protein catabolic process [Evidence
ISS] [PMID 9531531]"
/codon_start=1
/product="cullin CUL3"
/protein_id="XP_018736407.1"
/db_xref="GeneID:30037061"
/translation="MPKRKASSLWSPSSSPNSLKKTSSSGKVYAKAANIRTSTDLPTS
STSLPGSGSGSSSPLFEMQSSFPGPDLPISNQSSINNSRKLQNAIVGNRLLYGRAKPQ
TTRRLVIKSSSTTSDDSSTGNDNGKGKEPISGSGRARKMSSDAATKYYEETMEKLKTA
LEDILSPGLVGVAGIDKSELIPDSRGISQEELYRGVENLYRYNKAAILADLCLNTIEK
YAKKLPDVLQPHLESLTAHPLVRPMFGLDSELSSASASSNSSAHSLLDTNTPDAQAVS
HLYPFLRALDRLWKSWSQKIILVRNIFFYLDRAYLLPPQNKKKPIWDTGVDWFSKLIL
QDDVFNTIILTSISQIVNSYRVSLQSELSVVENISRMLASFKDDRSLLENEILSSAKE
LTNKDIDIKTIGIESFVDMLLNQITIEQRLGESLNFSTKFISTMVDNMKALLLKSRYK
DVFANLKVLLDKTDYERFQSLYTFSRELDLDTELLAAFRGYVINEGTSVLENNSAKST
KKGGSNLILSLLELYHRCLEIVRRGTKFPEPYELVDSNYEDAFRRAFAEFLNKDRSGM
VAEKLAKYADELLRSGIKACSEKELDEKFDELILIFRCVQGKDVFEAFYKKDFAKRLL
QSKSGGLDFEKSMISKLKVECGPSFTSRLETMIKDMELSKTYATEFREQAKSSKVKSI
AFNANILTQGHWPTLPETKIVLPPALVEYEKEFEKYYLAGKARRKLSWQHGLGNCIVK
ANFPRGTKELVLNLIQAVVLLQFNTFKDGVTTDEQVSYSQMKQSTQLDDSTLRRTLQS
LACGKVRILVKDPATREVDENDTFKVNLEFSSPVYKIKVSQIAAAEAGPKEAKKIHED
VNRDRQMEIQAAIIRLMKARKKLKHVELVQQTIDQIKERGLPNVQDIKKNIEKLLERE
YLERVDNTDTYIYV"
gene <825460..>826128
/gene="TRS33"
/locus_tag="AWJ20_4882"
/db_xref="GeneID:30037062"
mRNA <825460..>826128
/gene="TRS33"
/locus_tag="AWJ20_4882"
/product="Trs33p"
/transcript_id="XM_018881984.1"
/db_xref="GeneID:30037062"
CDS 825460..826128
/gene="TRS33"
/locus_tag="AWJ20_4882"
/note="Core component of transport protein particle
(TRAPP) complexes I-III; TRAPP complexes are related
multimeric guanine nucleotide-exchange factor for the
GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI),
intra-Golgi traffic (TRAPPII), endosome-Golgi traffic
(TRAPPII and III) and autophagy (TRAPPIII); GO_component:
GO:0005794 - Golgi apparatus [Evidence IEA,IEA];
GO_component: GO:1990070 - TRAPPI protein complex
[Evidence IDA] [PMID 11239471]; GO_component: GO:1990071 -
TRAPPII protein complex [Evidence IDA] [PMID 11239471];
GO_component: GO:1990072 - TRAPPIII protein complex
[Evidence IDA] [PMID 20375281]; GO_component: GO:0005801 -
cis-Golgi network [Evidence IDA] [PMID 19843283];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA,IEA]; GO_component: GO:0000407 - pre-autophagosomal
structure [Evidence IEA]; GO_component: GO:0005802 -
trans-Golgi network [Evidence IDA] [PMID 19843283];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0006888 - ER to Golgi vesicle-mediated
transport [Evidence IGI] [PMID 9564032]; GO_process:
GO:0006914 - autophagy [Evidence IEA]; GO_process:
GO:0006461 - protein complex assembly [Evidence IGI] [PMID
19843283]; GO_process: GO:0006810 - transport [Evidence
IEA]; GO_process: GO:0016192 - vesicle-mediated transport
[Evidence IEA]"
/codon_start=1
/product="Trs33p"
/protein_id="XP_018736408.1"
/db_xref="GeneID:30037062"
/translation="MESDSPYINGTNKLVAASSLDFLLIEIVPLAQSIVQQLIEDAGA
RPGRPGSSSSANGGGAGGAGGSNVSGGISGTSSSPANSSMTADENLDDVYYRIEGYGH
RVGLGLSQIFTKDRPWFTDQLDIMKFVCKELWVILYKKQIDNLKTNHRGTYVLTDTQF
KYCSRMGTAYGPKETLRRATPYLWFPAGVIRGALSALGIDAAVTFDADQLPKVTFNIQ
TSPT"
gene complement(<826385..>827491)
/gene="OCA1"
/locus_tag="AWJ20_4883"
/db_xref="GeneID:30037063"
mRNA complement(<826385..>827491)
/gene="OCA1"
/locus_tag="AWJ20_4883"
/product="putative tyrosine protein phosphatase OCA1"
/transcript_id="XM_018881985.1"
/db_xref="GeneID:30037063"
CDS complement(826385..827491)
/gene="OCA1"
/locus_tag="AWJ20_4883"
/inference="similar to AA sequence:KEGG_Orthology:K18043"
/codon_start=1
/product="putative tyrosine protein phosphatase OCA1"
/protein_id="XP_018736409.1"
/db_xref="GeneID:30037063"
/translation="MSIPSQSNGELASGQNNPAISSSIESQQQKSSGVSGAIDRTNGL
TNTNALHSGADTTAPTTSTTSTTYSSSTRTNKTNTDSLLNTGTGSTNTLTDSITNTIG
SVIAVADTGSSVVPNTSTEHNTTILLDQNTTTTNDSGTGSRNNGSSNNITTSTTSNST
TNDSTISSNTTSSNNTTVDKSSTTLAEIRRPPPVRLVPPLNFALAERQLYRSGQPAPI
NFPFLRGLNLKTIVWLAVEDPSDAFLGFADNNEIEFHHLGLVTEGGNPWDQLTESSIV
SALDIIMNVNNYPLLVCCGMGRHRTGTVIGCLRRVQGWNFASISDEYRRFVGAKGGRV
LIELHIEAFDTKSVHINLDYAPDWFKKTLPPPGA"
gene complement(<829550..>830287)
/gene="RAS1"
/locus_tag="AWJ20_4884"
/db_xref="GeneID:30037064"
mRNA complement(<829550..>830287)
/gene="RAS1"
/locus_tag="AWJ20_4884"
/product="Ras family GTPase RAS1"
/transcript_id="XM_018881986.1"
/db_xref="GeneID:30037064"
CDS complement(829550..830287)
/gene="RAS1"
/locus_tag="AWJ20_4884"
/inference="similar to AA sequence:KEGG_Orthology:K07827"
/note="GTPase involved in G-protein signaling in adenylate
cyclase activation; plays a role in cell proliferation;
localized to the plasma membrane; homolog of mammalian RAS
proto-oncogenes; relative distribution to the nucleus
increases upon DNA replication stress; RAS1 has a paralog,
RAS2, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
22842922]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 22842922]; GO_component: GO:0005886 - plasma
membrane [Evidence IEA,IEA]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 20162532];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 3513173]; GO_function: GO:0005525 - GTP binding
[Evidence IEA,IEA]; GO_function: GO:0003924 - GTPase
activity [Evidence IDA] [PMID 14674766]; GO_function:
GO:0003924 - GTPase activity [Evidence IMP] [PMID
6327067]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_process: GO:0006184 - GTP catabolic
process [Evidence IEA]; GO_process: GO:0007189 - adenylate
cyclase-activating G-protein coupled receptor signaling
pathway [Evidence IDA] [PMID 3891097]; GO_process:
GO:0045762 - positive regulation of adenylate cyclase
activity [Evidence IGI] [PMID 2981630]; GO_process:
GO:0045762 - positive regulation of adenylate cyclase
activity [Evidence IGI] [PMID 3891097]; GO_process:
GO:0097271 - protein localization to bud neck [Evidence
IGI] [PMID 12782684]; GO_process: GO:0001302 - replicative
cell aging [Evidence IMP] [PMID 8034612]; GO_process:
GO:0007165 - signal transduction [Evidence IEA];
GO_process: GO:0007264 - small GTPase mediated signal
transduction [Evidence IEA]"
/codon_start=1
/product="Ras family GTPase RAS1"
/protein_id="XP_018736410.1"
/db_xref="GeneID:30037064"
/translation="MLVREYKLVVVGGGGVGKSALTIQLIQSQFVDEYDPTIEDSYRK
QCLIDDEVVILDVLDTAGQEEYSAMREQYMRTGEGFLIVYSVTSRSSFDEVKNFHQQI
LRVKDQDAFPIVIIGNKIDLENERQVSFEEGQQLADSLGCPFLETSAKRRINVDDSFY
ALVTEVRKFNRGDSGSERTGSIKSKPSRPNNTSRSGTTTLNHEAAYYSGNTIGGGPTN
MAARSKRDPGTRYDEKPDNDGCCCIIS"
gene complement(<838397..>840295)
/gene="PHO23"
/locus_tag="AWJ20_4885"
/db_xref="GeneID:30037065"
mRNA complement(<838397..>840295)
/gene="PHO23"
/locus_tag="AWJ20_4885"
/product="Pho23p"
/transcript_id="XM_018881987.1"
/db_xref="GeneID:30037065"
CDS complement(838397..840295)
/gene="PHO23"
/locus_tag="AWJ20_4885"
/note="Component of the Rpd3L histone deacetylase complex;
involved in transcriptional regulation of PHO5; affects
termination of snoRNAs and cryptic unstable transcripts
(CUTs); C-terminus has similarity to human candidate tumor
suppressor p33(ING1) and its isoform ING3; GO_component:
GO:0033698 - Rpd3L complex [Evidence IDA] [PMID 16286007];
GO_component: GO:0033698 - Rpd3L complex [Evidence IDA]
[PMID 16286008]; GO_component: GO:0033698 - Rpd3L complex
[Evidence IDA] [PMID 16314178]; GO_component: GO:0033698 -
Rpd3L complex [Evidence IDA] [PMID 19040720];
GO_component: GO:0070210 - Rpd3L-Expanded complex
[Evidence IDA] [PMID 19040720]; GO_component: GO:0000118 -
histone deacetylase complex [Evidence IDA] [PMID
12672825]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 11914276]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0035064 -
methylated histone binding [Evidence IDA] [PMID 16728974];
GO_function: GO:0035064 - methylated histone binding
[Evidence IDA] [PMID 17142463]; GO_function: GO:0008270 -
zinc ion binding [Evidence IEA]; GO_process: GO:0016568 -
chromatin modification [Evidence IEA,IEA]; GO_process:
GO:0016568 - chromatin modification [Evidence IMP,IPI,ISS]
[PMID 10805724]; GO_process: GO:0061188 - negative
regulation of chromatin silencing at rDNA [Evidence IMP]
[PMID 16286008]; GO_process: GO:0061186 - negative
regulation of chromatin silencing at silent mating-type
cassette [Evidence IMP] [PMID 16286008]; GO_process:
GO:0031939 - negative regulation of chromatin silencing at
telomere [Evidence IMP] [PMID 16286008]; GO_process:
GO:0031939 - negative regulation of chromatin silencing at
telomere [Evidence IMP] [PMID 16314178]; GO_process:
GO:0031939 - negative regulation of chromatin silencing at
telomere [Evidence IMP] [PMID 19372273]; GO_process:
GO:0016479 - negative regulation of transcription from RNA
polymerase I promoter [Evidence IMP] [PMID 19270272];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 17210643]; GO_process: GO:0061408 - positive
regulation of transcription from RNA polymerase II
promoter in response to heat stress [Evidence IMP] [PMID
20398213]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Pho23p"
/protein_id="XP_018736411.1"
/db_xref="GeneID:30037065"
/translation="MEEQEQEVAYRGGGRLGKRNAAGQLTRAAPTDIYPGLNDMSDAL
EAIPMDIVRHFTLLKEIDAKCVGTTPLLGELIVEFLSLPVPSPATLQAALAASSSFGS
NEMQGQEPTNPAAAGSATGSGVAPGNAPVNGENDTSNSINNGNNNNNNNGDGNPANNN
SNGNNGDNSSNIGNNVQESQTDGASNNTANSGNTSAEPAVTGSNVLTVSEEEIASIVK
REELLAQIRRLINELVPCLEEKMHVAGVAADAMARHVARIDYDFDLICNNEIPERIQV
GSANHPAFIAETRLANEQQKGNQTSRSESRREAMAAKKAAAAADGLTGSAATGSGGAK
GGRNSTPVAGKGSGSAVSSAGSTTAGGAGSAFGSGATSGRGRGAGKNNGSASAGGNVS
ANGGAAGSGSVSSNYGNGLSHSNTPAPKRRKGAASAGNTTAGQPSALSNNSSNATAYN
SYNYEYQQSYAGQPGTNNGPGRPAGSNNGSAAAGNAGQTNQYNEYANGAGNTGTGSYN
GQGYNSNSNENSVIDGYEGSKYENEESISRPTTPAGGNGRRGGRQRTRTVGANSNSSS
ANAKDDGEPVYCYCQQVSFGEMVGCDGPECKREWFHLPCIGLSSPPKGQWFCEDCAAK
YKKPAVKR"
gene <840788..>842791
/gene="AEP3"
/locus_tag="AWJ20_4886"
/db_xref="GeneID:30037066"
mRNA <840788..>842791
/gene="AEP3"
/locus_tag="AWJ20_4886"
/product="Aep3p"
/transcript_id="XM_018881988.1"
/db_xref="GeneID:30037066"
CDS 840788..842791
/gene="AEP3"
/locus_tag="AWJ20_4886"
/note="Peripheral mitochondrial inner membrane protein;
may facilitate use of unformylated tRNA-Met in
mitochondrial translation initiation; stabilizes the
bicistronic AAP1-ATP6 mRNA; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11914276]; GO_component:
GO:0019898 - extrinsic component of membrane [Evidence
IDA] [PMID 14742425]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005743 - mitochondrial
inner membrane [Evidence IEA,IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence IDA]
[PMID 14742425]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0016071 -
mRNA metabolic process [Evidence IMP] [PMID 14742425];
GO_process: GO:0070124 - mitochondrial translational
initiation [Evidence IGI] [PMID 19843529]"
/codon_start=1
/product="Aep3p"
/protein_id="XP_018736412.1"
/db_xref="GeneID:30037066"
/translation="MKNVSGQVLFPVARSLRRSFLVHRRTASTSTHGQHVPEKSDPTS
DLVADVGKSATDSLEKTEKLPEKESLQSPNLLQLSPDLPKFYQKLNDELRRYHQLMPL
RTSAGDETQSHSQNTLSTLLTMEQEAKIELDQTANANERERQKKKEKEHRQLSRQTQI
NASPTYIQSIIEVLTSMRTALTSSNNEMVLERKFTSKPLPKFPTQWSREGLVAYLQQL
TQYQHTNSKYSSPNGLISSIVKDLLRVNNPSTMNHLSTEAFNIGISYHARISDIRSCR
QLFQDMLSSGISVNTDTYNILLMSCRYRLGNVNDNWKVHPLRVVDGFLKQMRSKGIPA
NIDTWNIVLGTVPEGSNKSRVLTEMSKKNVPLSRRGLATCVGDIVQQVGPKLAMVYLD
KQLEKYNVGIDTVNTIISGLLNDDKLKADKAGIKFTPSVETVDVAWKYLHYAISKWDL
TPSTSVLNTFGRFFAANGKLDWFIGLYSAMVSHPEWNIQPNCITYQYLLEAAVRVPFH
PYKYEMVETINRISYKVPRTKQYQNWLLRAKTQFDYYNSLNLLSKNTFSMNRHATPET
TQNFVQAQHILKWADQTFKPDYTNVLPRLQIAKLLNLFPSDIPAEETQQHTRQSRRVY
SQTTKQFRATTSTQETATKQRLIRKGPYQKYIDDLEEEGLIEG"
gene <846302..>849751
/gene="NCR1"
/locus_tag="AWJ20_4887"
/db_xref="GeneID:30037067"
mRNA <846302..>849751
/gene="NCR1"
/locus_tag="AWJ20_4887"
/product="Ncr1p"
/transcript_id="XM_018881989.1"
/db_xref="GeneID:30037067"
CDS 846302..849751
/gene="NCR1"
/locus_tag="AWJ20_4887"
/inference="similar to AA sequence:KEGG_Orthology:K12385"
/note="Vacuolar membrane protein; transits through the
biosynthetic vacuolar protein sorting pathway, involved in
sphingolipid metabolism; cells lacking Ncr1p exhibit high
levels of long chain bases (LCB), similar to the
accumulation of high amounts of lipids observed in
patients with Neimann-Pick C, a disease caused by
loss-of-function mutations in NPC1, the functional
ortholog of Ncr1p; GO_component: GO:0000329 - fungal-type
vacuole membrane [Evidence IDA] [PMID 14562095];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IDA] [PMID 16138904]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005774 -
vacuolar membrane [Evidence IEA]; GO_component: GO:0005773
- vacuole [Evidence IEA]; GO_function: GO:0008158 -
hedgehog receptor activity [Evidence IEA]; GO_function:
GO:0046624 - sphingolipid transporter activity [Evidence
IMP] [PMID 14970192]; GO_process: GO:0006629 - lipid
metabolic process [Evidence IEA]; GO_process: GO:0006869 -
lipid transport [Evidence IEA]; GO_process: GO:0007165 -
signal transduction [Evidence IEA]; GO_process: GO:0006665
- sphingolipid metabolic process [Evidence IEA];
GO_process: GO:0006665 - sphingolipid metabolic process
[Evidence IMP] [PMID 14970192]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Ncr1p"
/protein_id="XP_018736413.1"
/db_xref="GeneID:30037067"
/translation="MNFPWLNKTDEDSRDPEGVKRSDVDMHSCGDEDERYRCACSDCS
VACPELEPVRDGGKGRLAVSRCTLFGGRVLCFSLAVWVVYVILLSFLLMGLWGYRLVR
GRVVKANESLSLLYDDGEIDSEDEYEDDFYQRPAFLRRHSIGTRRTSDGLEEDDDYDD
DDDDANDNRRVANVIYNPRHHHSTAPYKLNTVLQDGFSRLGLICASFPAVVISFSLLV
VVILSFGLIRFQLEIDPVKLWVSPTSLEYSEKQHFDSNFGPFYRAQQMYLVNDTGPVL
SDYGTLQWWFSVEERIGRLRSSHYGVSYNDLCLKPLGDACVIQSVTQYFGGDINAVPE
NWQDKLQSCADSPVSCLPPFQQPLKKEMVFGGYIGDDVITSKALVISLVVENSDDPEK
LAVAQEWEELLEMFLFSVQKEAAYRGLRLSFNVEISLEKELNKSSNTDVRIVVLSYVF
MFLYASLALGSVTPQLSRRYLVKSKFSLGLFGIFVVLLSVISSVGLFSYFGIKCTLII
AEVIPFLILAVGVDNIFLLTHGLETVNLTHPNLSIEERLALALGHVGPSILLSTLCEV
SAFALGASVAMPAVRNFAIYSAGAVFFNSLLQMTMFMSALSLDQRRSEDDRADCFPCI
RIPRISGISRPSFSSFTARVLSSPSIFDGQPGPLPSGNHGGTNVFSDIIRKKYAPYIL
SPQVKPIVIAIFTGWLAISLSLLPHIELGLDQRLAVPSDSYLVDYFNDLYDYFGSGPP
VYFVTHDFKVTDRSSQQALCGRFSTCKEFSLMNILEQERKRPAVSYINDPAASWIDDF
LQWLNPDLDDCCRFKKGTNETEVCAPHASPRTCEVCYLDKKWDITMGGLPNGDEEFLK
YFNIWIEAPSDPCPLGGKAPYSHSVVVDDDSSSISTSNFRTAHTPLRSQSDFINAYAS
ARRIAQSIEDHTGVPTYPYSTFYIFFAQYSTIIYDTFRLLSVALFITFILSTILLGSI
RTAFSVCIVVACIVTNIGGIMALWDISLNAVSLVNLVICVGIGIEFCIHVARAYTFTP
RMDIAGYTAVSKEMRAFNALTGVGGSVFGGIALTKLIGVCVLAFTRSKIFEVYYFRMW
TALVLVASTHSLVFLPVVLTYIGGRGYLIDGGDEGVPGDLASRLYGSEENYGTEPEED
FLD"
gene complement(<850029..>850099)
/locus_tag="AWJ20_4888"
/db_xref="GeneID:30037068"
tRNA complement(<850029..>850099)
/locus_tag="AWJ20_4888"
/product="tRNA-Gly"
/db_xref="GeneID:30037068"
gene <850799..>851839
/gene="HMT1"
/locus_tag="AWJ20_4889"
/db_xref="GeneID:30037069"
mRNA <850799..>851839
/gene="HMT1"
/locus_tag="AWJ20_4889"
/product="protein-arginine omega-N methyltransferase HMT1"
/transcript_id="XM_018881990.1"
/db_xref="GeneID:30037069"
CDS 850799..851839
/gene="HMT1"
/locus_tag="AWJ20_4889"
/inference="similar to AA sequence:KEGG_Orthology:K11434"
/note="Nuclear SAM-dependent mono- and asymmetric
methyltransferase; modifies hnRNPs, including Npl3p and
Hrp1p, affecting their activity and nuclear export;
methylates U1 snRNP protein Snp1p and ribosomal protein
Rps2p; interacts genetically with genes encoding
components of Rpd3(L) and this interaction is important
for Rpd3 recruitment to the subtelomeric region;
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
8668183]; GO_function: GO:0008168 - methyltransferase
activity [Evidence IEA,IEA]; GO_function: GO:0035242 -
protein-arginine omega-N asymmetric methyltransferase
activity [Evidence IDA,IMP] [PMID 8647869]; GO_function:
GO:0035241 - protein-arginine omega-N
monomethyltransferase activity [Evidence IDA,IMP] [PMID
8647869]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0006406 - mRNA export from
nucleus [Evidence IGI] [PMID 10952997]; GO_process:
GO:0006406 - mRNA export from nucleus [Evidence IGI] [PMID
8668183]; GO_process: GO:0032259 - methylation [Evidence
IEA]; GO_process: GO:0060567 - negative regulation of
DNA-templated transcription, termination [Evidence IMP]
[PMID 20053728]; GO_process: GO:0018216 -
peptidyl-arginine methylation [Evidence IMP] [PMID
18515076]; GO_process: GO:0018216 - peptidyl-arginine
methylation [Evidence IMP] [PMID 20035717]; GO_process:
GO:0018216 - peptidyl-arginine methylation [Evidence IDA]
[PMID 22997150]; GO_process: GO:0018216 -
peptidyl-arginine methylation [Evidence IDA,IMP] [PMID
8647869]; GO_process: GO:0032968 - positive regulation of
transcription elongation from RNA polymerase II promoter
[Evidence IMP] [PMID 20053728]; GO_process: GO:0006479 -
protein methylation [Evidence IEA]"
/codon_start=1
/product="protein-arginine omega-N methyltransferase HMT1"
/protein_id="XP_018736414.1"
/db_xref="GeneID:30037069"
/translation="MSDSMTDVSPAPEASNGDLNYSDQHYFNSYDHFGIHEEMLKDDV
RTTSYRNAIYQNRHMFKDKVVLDVGCGTGILSMFAARSGAKHVIGVDMSNIIHMARKI
VDLNGFSNKITLLHGKMEEVELPYPKVDIIISEWMGYFLLYESMLDTVLIARDKYLVE
GGLIFPDKATIYAAAIEDGDYKEEKIGFWNDVYGFDYSPFQELALADPLVDTVELRAV
ITDPCPIFDLDLYTITIEDLAFSREFSLKARRDDIAHAIVAWFDIEFSKCHKPIKFST
GPHAKYTHWKQTVFYLADEVLIKNGEYITGRLTSGPNSENPRNLDITITHKLETEDEK
RKSEGEAHYVMK"
gene complement(<852123..>855020)
/gene="VHC1"
/locus_tag="AWJ20_4890"
/db_xref="GeneID:30037071"
mRNA complement(<852123..>855020)
/gene="VHC1"
/locus_tag="AWJ20_4890"
/product="Vhc1p"
/transcript_id="XM_018881992.1"
/db_xref="GeneID:30037071"
CDS complement(852123..855020)
/gene="VHC1"
/locus_tag="AWJ20_4890"
/inference="similar to AA sequence:KEGG_Orthology:K14429"
/note="Vacuolar membrane cation-chloride cotransporter
(CCC); likely mediates K+ and Cl- cotransport into the
vacuole; has a role in potassium homeostasis and salt
tolerance; similar to mammalian electroneutral
Na(+)-(K+)-C1- cotransporter family; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 19001347]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0031166 - integral
component of vacuolar membrane [Evidence IDA] [PMID
23022132]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_function: GO:0015379 - potassium:chloride
symporter activity [Evidence IMP,ISA] [PMID 23022132];
GO_process: GO:0055075 - potassium ion homeostasis
[Evidence IGI] [PMID 23022132]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]; GO_process:
GO:0034486 - vacuolar transmembrane transport [Evidence
IMP] [PMID 23022132]"
/codon_start=1
/product="Vhc1p"
/protein_id="XP_018736415.1"
/db_xref="GeneID:30037071"
/translation="MDSKFGGAAYTGLSWSTFKDNFFPRFTSGAAGSQLPGKENFQDM
FGIFFPATSGIFAGASMSGDLKAPSKSIPKGTLWGLLVTFICYCLVILSIGSTVSRDL
LYRDLEIIRNSNLSPMLIIMGEFSTSLFSALMGVVGAAKLLQAIARDNLLPYTSWFGK
GYSTDDNPLPAIFLSYILTQITLLFDINQIAVFITMAYLMTFVVTNLACFLLKIASAP
NFRPSFKYFNSTSAGLGAALCVLSMFVADGISASAMICVIAMLFLTIHYISPPKPWGD
VSQTLIYHQVRKYLLRLRQDHVKFWRPQILLLVDDPRTSWSLIHFCNHLKKGGLYILG
HVMVTQDFTQSFPEMKKQQNSWMKLRDVSNIKGFVQIGAGPNVVWGARNVYLGSGLGG
MKPNITVLGFFELKNHYPSRHNNSHCRGDESLTGQHRSDEAIQIEDLPTDSCRKEPSI
TVTEWVHIIEDLLTMQANIAIAKGFPRLEFPTKESLNASLSDTKGYIDLYPIQMSAQI
VDENGAMSALSTNFDTYTLILQLGAILHTVPVWKKNHILRVVVFVEFGEDVASEESRI
KLLLEKLRINAEVLVLCLSAGNVCAYETIIHGKPDTSGRVHRLLGEDEWWIELQEARS
SNIDHGIHINSNVGDLIPDENLGNEANHQLRKIIDAKKRRHTVSELQRLGVSFSMHTN
NLMKSNLTKHGYDSDSSSSDSEVEFEDADDSLEMAEPRLFVRRRASSGGSLPGSTRPV
FTTPSQPTSEVARQIAAAALSAKQTKPSLIRTNSGTGSIKSKKLKPHFLGNTMPDTRV
VEEPEAGRRSIMFDETSTAPSSNRPSRPSSIKSGLSGLSTNKVTFDEQPAAALSQKSP
LSTMTSQKVSSSSNTSLTLTDTGVPEDEERDLSFNDLPAKAQHVILNDMMNQTSKDSV
VIFSTLPAPSPGTHKSEDESLEYVESLELWCQDLPPMILLHSQSMTVTTAL"
gene complement(<855368..>856006)
/gene="VHC1"
/locus_tag="AWJ20_4891"
/db_xref="GeneID:30037072"
mRNA complement(<855368..>856006)
/gene="VHC1"
/locus_tag="AWJ20_4891"
/product="Vhc1p"
/transcript_id="XM_018881993.1"
/db_xref="GeneID:30037072"
CDS complement(855368..856006)
/gene="VHC1"
/locus_tag="AWJ20_4891"
/note="Vacuolar membrane cation-chloride cotransporter
(CCC); likely mediates K+ and Cl- cotransport into the
vacuole; has a role in potassium homeostasis and salt
tolerance; similar to mammalian electroneutral
Na(+)-(K+)-C1- cotransporter family; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 19001347]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0031166 - integral
component of vacuolar membrane [Evidence IDA] [PMID
23022132]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_function: GO:0015379 - potassium:chloride
symporter activity [Evidence IMP,ISA] [PMID 23022132];
GO_process: GO:0055075 - potassium ion homeostasis
[Evidence IGI] [PMID 23022132]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]; GO_process:
GO:0034486 - vacuolar transmembrane transport [Evidence
IMP] [PMID 23022132]"
/codon_start=1
/product="Vhc1p"
/protein_id="XP_018736416.1"
/db_xref="GeneID:30037072"
/translation="MAPGGLSAGGKSSRPGTGSDVSSEISRSTSPKIALDDSVLLSAN
AIHGLDSRGGDSSDYDTVTSKTGLLHRKSKVKYGSTGGESFEPSHSLNRSITVVEEQQ
EKLHRNSQYSKMNTLEGVFVPTVLNVLSILMFLRFGFILGQTGILGMMVLLLASYGVN
LLTTLSISAISTNGTVRGGGAYYMISRSLGPEFGGSIGVIFYIGQVLNRCVL"
gene complement(<857008..>858786)
/gene="ABD1"
/locus_tag="AWJ20_4892"
/db_xref="GeneID:30037073"
mRNA complement(<857008..>858786)
/gene="ABD1"
/locus_tag="AWJ20_4892"
/product="Abd1p"
/transcript_id="XM_018881994.1"
/db_xref="GeneID:30037073"
CDS complement(857008..858786)
/gene="ABD1"
/locus_tag="AWJ20_4892"
/inference="similar to AA sequence:KEGG_Orthology:K00565"
/note="Methyltransferase; catalyzes the transfer of a
methyl group from S-adenosylmethionine to the GpppN
terminus of capped mRNA; nuclear protein that relocalizes
to the cytosol in response to hypoxia; GO_component:
GO:0016591 - DNA-directed RNA polymerase II, holoenzyme
[Evidence IPI] [PMID 11018011]; GO_component: GO:0005829 -
cytosol [Evidence IDA] [PMID 22932476]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IPI] [PMID 11018011];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22932476]; GO_function: GO:0003723 - RNA binding [Evidence
IEA]; GO_function: GO:0004482 - mRNA
(guanine-N7-)-methyltransferase activity [Evidence
IEA,IEA]; GO_function: GO:0004482 - mRNA
(guanine-N7-)-methyltransferase activity [Evidence
IDA,ISS] [PMID 7623811]; GO_function: GO:0008168 -
methyltransferase activity [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0006370 - 7-methylguanosine mRNA capping
[Evidence IEA,IEA]; GO_process: GO:0006370 -
7-methylguanosine mRNA capping [Evidence IDA] [PMID
7623811]; GO_process: GO:0036265 - RNA
(guanine-N7)-methylation [Evidence IEA,IEA]; GO_process:
GO:0006397 - mRNA processing [Evidence IEA]; GO_process:
GO:0032259 - methylation [Evidence IEA]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IMP] [PMID 14967145]"
/codon_start=1
/product="Abd1p"
/protein_id="XP_018736417.1"
/db_xref="GeneID:30037073"
/translation="MYDPARDAWNASTENSADEKDLEKVPNVGGDGDESGDTEDEAPV
RIETRGIETHDQNNDDYDGSTEQPESLVDRENNGKSRTSTGKAVIEAVEAVAEAAVDT
LTKAASATAAVLAEPVLSELDGEVIDEKNSQSASSTASQKSTVDEREKRADVKKQRSP
SPTSPPKELKKPSQRADVGERDREALEPDEKLNRTSANGGEKKENISEDSNNSEFRST
SRTTESTESKEETHSTSQKSTIDDKETTDNVKRQRSPSPASPPRKLKKPSRRSDVGER
DRKAQELREKLDKAAADDIRAHYNERPNKSIEERTQSPIIKLRSFNNLIKSVLIQIFS
RPRQVVLDIGCGKGGDLQKWSKQRISGLIGVDIADISIEQAKERYEHLRQKSFWADFC
VGDAFERTIEDIVHPDAFPVDFVSSQFTIHYSFENEQRARTMLSNVSRALKPGGTFIG
TTPDSDRLLEHISKLPAGVREWGNSAYRIQFEDEPPKSNDDLKDIFGYKYNFFLDDAV
TNVVEFMVPFEAFTELAKEYGLRLVLRRNFLDFFNDELASRRGRDRILFFCRILKVYK
EDGTPGIEGDQREAAGIYTVFAFEKE"
gene complement(<865737..>866093)
/gene="CMC1"
/locus_tag="AWJ20_4893"
/db_xref="GeneID:30037074"
mRNA complement(<865737..>866093)
/gene="CMC1"
/locus_tag="AWJ20_4893"
/product="COX assembly mitochondrial protein"
/transcript_id="XM_018881995.1"
/db_xref="GeneID:30037074"
CDS complement(865737..866093)
/gene="CMC1"
/locus_tag="AWJ20_4893"
/inference="similar to AA sequence:KEGG_Orthology:K18171"
/codon_start=1
/product="COX assembly mitochondrial protein"
/protein_id="XP_018736418.1"
/db_xref="GeneID:30037074"
/translation="MSSTPATAHTNLSSETSSTTATSASSGRKVPAWMLAPYEEKEVR
ARCQEQADRMCADKFLAFGRCSEKNQLLFSWKCAEQKKAMIDCVAYWGSTEKFEEVQD
RYITEKQEKLRKEGRL"
gene <866778..>867416
/gene="spc24"
/locus_tag="AWJ20_4894"
/db_xref="GeneID:30037075"
mRNA <866778..>867416
/gene="spc24"
/locus_tag="AWJ20_4894"
/product="spindle pole body protein Spc24"
/transcript_id="XM_018881996.1"
/db_xref="GeneID:30037075"
CDS 866778..867416
/gene="spc24"
/locus_tag="AWJ20_4894"
/inference="similar to AA sequence:KEGG_Orthology:K11557"
/codon_start=1
/product="spindle pole body protein Spc24"
/protein_id="XP_018736419.1"
/db_xref="GeneID:30037075"
/translation="MSDELGNYFPLIQSTMEHFEIQEDIDTCDRTEQMFQRIQEKRKK
ITDESYDTLRALSRKLELAKAKTDPSEKSAAHREATQRLVQLDREKFALAKSLNELEQ
SNTSSQTTLDILRQELEDLKDADSIEETSSQYVDNAAVLKLKFYRSLGLRFSDDEDET
GLGSGKTSADGQSASTGLRVFIQSSTENRVHVFSIDKNYSSQFVANYIWDHM"
gene complement(<867689..>869290)
/locus_tag="AWJ20_4895"
/db_xref="GeneID:30037076"
mRNA complement(<867689..>869290)
/locus_tag="AWJ20_4895"
/product="Uncharacterized protein YKL098W"
/transcript_id="XM_018881997.1"
/db_xref="GeneID:30037076"
CDS complement(867689..869290)
/locus_tag="AWJ20_4895"
/codon_start=1
/product="Uncharacterized protein YKL098W"
/protein_id="XP_018736420.1"
/db_xref="GeneID:30037076"
/translation="MVGSFASLCEQVSVEYENFDVNVLIALVVAAVGRRHLVIQSKRV
SATREVLQTLARDVLGFREDRATVVVHCNADTTWHQLAKSILVNGNKFSHETECEIAG
SGSPNLFGDGNGSGNVNNEESVKREGVNGSPNINDEGSVHPNVSVPTFLIIENLDKTA
KLVQTMMISTLVERKLVVNKKSYPLPEDFVLVIIGHGHNLVPHLRDYMLLEHVVETDD
DHGLNADQPAHTGQMSSAVGAGINSDNVGGGSANVSAGGSGNGSTSATAGTGNGIGGI
GISTGSTESVNTPLTDIDRRGMSDPQLVQPVTRASESNSVLTPKGYYSGGGEDDSYRR
HSETAIVNATTQQGSGLYPGKHDLSALTKSNTNISNKSTSSFGVGVVSRRPTLPDFSP
PPGLHSSDNADTLSFQNLLSSMADKIPLITIAANIQRYMQDIVVALRTHRLVRSGVSP
QAVKDFHYLVRALSVLHDYEFVTPSIVSISARKLFPLRIQLCEPTDEPSLSYGGDIDL
VTQWMNKWDEDLVIEEILANVPCAM"
gene <869640..>870392
/gene="UTP11"
/locus_tag="AWJ20_4896"
/db_xref="GeneID:30037077"
mRNA <869640..>870392
/gene="UTP11"
/locus_tag="AWJ20_4896"
/product="Utp11p"
/transcript_id="XM_018881998.1"
/db_xref="GeneID:30037077"
CDS 869640..870392
/gene="UTP11"
/locus_tag="AWJ20_4896"
/inference="similar to AA sequence:KEGG_Orthology:K14769"
/note="Subunit of U3-containing Small Subunit (SSU)
processome complex; involved in production of 18S rRNA and
assembly of small ribosomal subunit; GO_component:
GO:0005730 - nucleolus [Evidence IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 12068309];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0032040 - small-subunit
processome [Evidence IEA]; GO_component: GO:0032040 -
small-subunit processome [Evidence IDA] [PMID 12068309];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0000480 - endonucleolytic cleavage in
5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000447 - endonucleolytic cleavage in ITS1
to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 15489292]; GO_process:
GO:0000472 - endonucleolytic cleavage to generate mature
5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[Evidence IMP] [PMID 15489292]; GO_process: GO:0006364 -
rRNA processing [Evidence IEA,IEA]; GO_process: GO:0042254
- ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Utp11p"
/protein_id="XP_018736421.1"
/db_xref="GeneID:30037077"
/translation="MVALTHNIAKKQHRERAQPTERRKWGLLEKKKDYKLRSQDFHKK
ENHLKLLRRKATERNPDEFYHSMVNKKTDNRGILITERGNEVLSNGAAQLLKTQDSSY
VRTLTSGEIRKIEKLETELTFGGQGEHTVFVDSDSQAKSFDAAEYFGTHKSLLNRREN
RLRKNQLEEQDLSKTTLIPTDVDERTEQKLRKQKMAKYKELDQRLERQQQLSQVQQQM
DLQRELMKKGDKKKIITKDGKQTWKWKNVRKR"
gene complement(<871443..>873332)
/gene="HAS1"
/locus_tag="AWJ20_4897"
/db_xref="GeneID:30037078"
mRNA complement(<871443..>873332)
/gene="HAS1"
/locus_tag="AWJ20_4897"
/product="ATP-dependent RNA helicase HAS1"
/transcript_id="XM_018881999.1"
/db_xref="GeneID:30037078"
CDS complement(871443..873332)
/gene="HAS1"
/locus_tag="AWJ20_4897"
/inference="similar to AA sequence:KEGG_Orthology:K13179"
/note="ATP-dependent RNA helicase; involved in the
biogenesis of 40S and 60S ribosome subunits; localizes to
both the nuclear periphery and nucleolus; highly enriched
in nuclear pore complex fractions; constituent of 66S
pre-ribosomal particles; GO_component: GO:0030686 - 90S
preribosome [Evidence IDA] [PMID 12150911]; GO_component:
GO:0005635 - nuclear envelope [Evidence IDA] [PMID
10684247]; GO_component: GO:0005730 - nucleolus [Evidence
IEA]; GO_component: GO:0005730 - nucleolus [Evidence IDA]
[PMID 10684247]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 15049817]; GO_component: GO:0005634 -
nucleus [Evidence IEA]; GO_component: GO:0030687 -
preribosome, large subunit precursor [Evidence IDA] [PMID
11583614]; GO_component: GO:0030687 - preribosome, large
subunit precursor [Evidence IDA] [PMID 17443350];
GO_function: GO:0005524 - ATP binding [Evidence IEA,IEA];
GO_function: GO:0004004 - ATP-dependent RNA helicase
activity [Evidence IDA] [PMID 15718299]; GO_function:
GO:0008026 - ATP-dependent helicase activity [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence IDA]
[PMID 15718299]; GO_function: GO:0008186 - RNA-dependent
ATPase activity [Evidence IDA] [PMID 15718299];
GO_function: GO:0004386 - helicase activity [Evidence
IEA,IEA]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0003676 - nucleic acid
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006200
- ATP catabolic process [Evidence IEA]; GO_process:
GO:0000463 - maturation of LSU-rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 23788678]; GO_process: GO:0000462 - maturation of
SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15049817];
GO_process: GO:0006364 - rRNA processing [Evidence IEA];
GO_process: GO:0006364 - rRNA processing [Evidence IMP]
[PMID 15242642]; GO_process: GO:0042273 - ribosomal large
subunit biogenesis [Evidence IMP] [PMID 15049817];
GO_process: GO:0042273 - ribosomal large subunit
biogenesis [Evidence IMP] [PMID 23788678]; GO_process:
GO:0042274 - ribosomal small subunit biogenesis [Evidence
IMP] [PMID 15049817]; GO_process: GO:0042274 - ribosomal
small subunit biogenesis [Evidence IMP] [PMID 23788678];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA]"
/codon_start=1
/product="ATP-dependent RNA helicase HAS1"
/protein_id="XP_018736422.1"
/db_xref="GeneID:30037078"
/translation="MSRKEKSVAVKSKKPRSSLSNGKSSSVKKTKAVEHSKKVASLKK
SRKQKEESPEESDIENDEDEVEDAEVEELDQEFDEVEGLLDVEAEENEDDEEEENEDD
EEENDDDEEEEEDEEKENGDENEFEDENGNITAPSKSAIVASSGSASGPDPTLFSELK
LSENTAKAITEMGFKKMTSVQARAIPPLLAGRDVLGAAKTGSGKTLAFLIPAIELLYS
LKFKPRNGTGVIIISPTRELALQIFGVARELMAHHTQTLGIVIGGANRRAEAEKLVKG
VNLIVATPGRLLDHLQNTKGFVFKNLKALVIDEADRILEIGFEDEMKQIIKILPQRDD
RQSMLFSATQTTKVEDLARISLRPGPLYINVDSEKETSTADGLEQGYVVCDSDKRFLL
LFSFLRRNKNKKVIVFLSSCNCVKFYGELLNYIDLPVLDLHGKQKQQKRTNTFFEFCN
AKQGILICTDVAARGLDIPAVDWIIQFDPPDDPRDYIHRVGRTARGTQGKGKSLMFLT
PSELGFLRYLKAAKVPLNEYEFPASKIANVQGQLEKLVSSNYWLNQSAKDGYRAYLQA
YSSHHLKTVYQIDKLDLVKVAKSFGFNIPPKVNITIGASGRAPKKNNGQNDYKKRKFN
NSSRR"
gene <875646..>877121
/gene="SUV3"
/locus_tag="AWJ20_4898"
/db_xref="GeneID:30037079"
mRNA <875646..>877121
/gene="SUV3"
/locus_tag="AWJ20_4898"
/product="Suv3p"
/transcript_id="XM_018882000.1"
/db_xref="GeneID:30037079"
CDS 875646..877121
/gene="SUV3"
/locus_tag="AWJ20_4898"
/inference="similar to AA sequence:KEGG_Orthology:K17675"
/note="ATP-dependent RNA helicase; component of the
mitochondrial degradosome along with the RNase Dss1p; the
degradosome associates with the ribosome and mediates RNA
turnover; also required during splicing of the COX1
AI5_beta intron; GO_component: GO:0000262 - mitochondrial
chromosome [Evidence IDA] [PMID 21911497]; GO_component:
GO:0045025 - mitochondrial degradosome [Evidence IDA]
[PMID 12426313]; GO_component: GO:0005759 - mitochondrial
matrix [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0004004 -
ATP-dependent RNA helicase activity [Evidence IDA] [PMID
12426313]; GO_function: GO:0004004 - ATP-dependent RNA
helicase activity [Evidence IDA] [PMID 17658549];
GO_function: GO:0003723 - RNA binding [Evidence IEA];
GO_function: GO:0008859 - exoribonuclease II activity
[Evidence IDA] [PMID 17658549]; GO_function: GO:0004386 -
helicase activity [Evidence IEA,IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0016817 - hydrolase activity, acting on
acid anhydrides [Evidence IEA]; GO_function: GO:0003676 -
nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0000372 - Group I intron splicing [Evidence
IGI] [PMID 20064926]; GO_process: GO:0006264 -
mitochondrial DNA replication [Evidence IMP] [PMID
21911497]; GO_process: GO:0000957 - mitochondrial RNA
catabolic process [Evidence IMP] [PMID 12426313]"
/codon_start=1
/product="Suv3p"
/protein_id="XP_018736423.1"
/db_xref="GeneID:30037079"
/translation="MKSARIPCNLVTGEEVIEEFDETGRPAGLCSGTVEMMDINRQMD
VAVLDEIQMIDDAERGWAWTQAFLAVQAREIHLCGDPNTEELVRKLAAQAGDELEVVN
YTRLSPLEIEKRELGDDLQKLQPGDCLVFFSKRHILAAKNIIEAETGDKCAVIYGSLP
AETRSKQAELFNDPDSDVKYLVASDAIGMGLNLAVRRIIFSRVRKFNGHELIKLPIAQ
VKQIAGRAGRFQTAPSKDSRDTRSTGNVGYVTSLHAHDREYIEQCLALPSPPVKQAAF
FPSDTIIQEFAHFIGPQSPYHQMLARLEASIKLPPLYKFVDLSNMITTSKIFANIRGL
LLEEMMTLAKAPVSSTDERVKSAFYSFCLVIANGQSKTITEIPNTGVGYLGMSVIPNA
RPSDTFESIHRVLSLYLWLSYRFPTHFLDRTGAMQLKELCETKINDLLKKSPSISRIV
GRKKAKSFKATALHDDSEPLNHLQPPSPRRPRAPTRRYSSF"
gene <877948..>878958
/gene="ARP2"
/locus_tag="AWJ20_4899"
/db_xref="GeneID:30037080"
mRNA <877948..>878958
/gene="ARP2"
/locus_tag="AWJ20_4899"
/product="actin-related protein 2"
/transcript_id="XM_018882001.1"
/db_xref="GeneID:30037080"
CDS 877948..878958
/gene="ARP2"
/locus_tag="AWJ20_4899"
/inference="similar to AA sequence:KEGG_Orthology:K17260"
/note="Essential component of the Arp2/3 complex; Arp2/3
is a highly conserved actin nucleation center required for
the motility and integrity of actin patches; involved in
endocytosis and membrane growth and polarity; required for
efficient Golgi-to-ER trafficking in COPI mutants;
GO_component: GO:0005885 - Arp2/3 protein complex
[Evidence IEA]; GO_component: GO:0005885 - Arp2/3 protein
complex [Evidence IDA] [PMID 10377407]; GO_component:
GO:0030479 - actin cortical patch [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IEA];
GO_component: GO:0005856 - cytoskeleton [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 11248049]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0005524 -
ATP binding [Evidence IDA,IMP] [PMID 15657399];
GO_function: GO:0016887 - ATPase activity [Evidence IMP]
[PMID 16862144]; GO_function: GO:0003779 - actin binding
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006200 - ATP
catabolic process [Evidence IMP] [PMID 16862144];
GO_process: GO:0034314 - Arp2/3 complex-mediated actin
nucleation [Evidence IEA]; GO_process: GO:0034314 - Arp2/3
complex-mediated actin nucleation [Evidence IMP] [PMID
16862144]; GO_process: GO:0032258 - CVT pathway [Evidence
IMP] [PMID 18287533]; GO_process: GO:0007015 - actin
filament organization [Evidence IMP] [PMID 10377407];
GO_process: GO:0030476 - ascospore wall assembly [Evidence
IMP] [PMID 17118118]; GO_process: GO:0051654 -
establishment of mitochondrion localization [Evidence IMP]
[PMID 16107558]; GO_process: GO:0000001 - mitochondrion
inheritance [Evidence IMP,IPI] [PMID 11248049];
GO_process: GO:0030833 - regulation of actin filament
polymerization [Evidence IEA]"
/codon_start=1
/product="actin-related protein 2"
/protein_id="XP_018736424.1"
/db_xref="GeneID:30037080"
/translation="MCGDEASAVRSALQVSYPMENGIIRNWEDMGHLWDYSFYERMKI
DPRGRKILLTEPPLNPVANREKMVGTMFEKYGFNGVYVAIQAVLALYAQGLSSGVVVD
SGDGVTHIVPVYESVVLNHLTRRLDIAGRDITRNLINLLLRRGYAFNRTADFETVRQI
KEKFCYVSYDLEYDDRLANETTALMETYELPDGRTIKLGPERFQAPECLFQPHLVDSE
QPGVAEFLFNTIQAADVDIRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWLTRVLNG
DPERLSKFKVRIEDPPRRRHMVFLGGAVLANIMHDKEHMWISKAEWEEHGANILSKLG
PR"
gene complement(<879174..>880973)
/locus_tag="AWJ20_4900"
/db_xref="GeneID:30037083"
mRNA complement(<879174..>880973)
/locus_tag="AWJ20_4900"
/product="hypothetical protein"
/transcript_id="XM_018882004.1"
/db_xref="GeneID:30037083"
CDS complement(879174..880973)
/locus_tag="AWJ20_4900"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736425.1"
/db_xref="GeneID:30037083"
/translation="MKDLITDFTSGVRLIQLLEIIGNEPLGRYNPNPRLNVQKAENIN
TALAFIKHRGILLHNIGAGDIIEGNLKLILGLLWILILRFTISEINEEGLSAKEGLLL
WCQRKTAGYPGVNIKDFSSSWTDGLAFCALMDRHRPDLIDFDELDKSNHKKNVSLAFK
LAAEEVGIPQLLDVEDICDVKSPDERSVMTYVAYWFHAFSALDKIETAGRRLEKFVDV
TSSALTMQHNFEKRMKVLIQSIKKTQESWAKSEFSGTYSDAKLQSSRFSEYKKTTKRE
WITEKSELASLLGNIRTKLATYGLREYVPPEGLRLQDLEQIWRELISAEATRSRAINN
TMRQIKEKLRKNFADTANELSLSLDTVSLKISDLEGELEKQLEGITILSDQLKPLDRM
LADLKKLDDQCLEANVEENDYTVYSLNELEYELGLAKQSVVKKLAFIENQIVARSMTN
LTPIQLEEFESVFRFFDKGQRNSLQESEFSGALASLGLVYSEEEMHQVFVVASGGNSR
VSFEEFIKFMVEVTEDQQTAEQVYQSFFDIANGKPYVTELDLQNSLIPDSMIDELKNS
MPLAKGANESEEEGVSLDYVTYMSQLTGVNGHK"
gene complement(<882712..>882801)
/locus_tag="AWJ20_4901"
/db_xref="GeneID:30037084"
tRNA complement(<882712..>882801)
/locus_tag="AWJ20_4901"
/product="tRNA-Phe"
/db_xref="GeneID:30037084"
gene <884710..>885375
/gene="hus1"
/locus_tag="AWJ20_4902"
/db_xref="GeneID:30037085"
mRNA <884710..>885375
/gene="hus1"
/locus_tag="AWJ20_4902"
/product="checkpoint clamp complex protein Hus1"
/transcript_id="XM_018882005.1"
/db_xref="GeneID:30037085"
CDS 884710..885375
/gene="hus1"
/locus_tag="AWJ20_4902"
/inference="similar to AA sequence:KEGG_Orthology:K10903"
/codon_start=1
/product="checkpoint clamp complex protein Hus1"
/protein_id="XP_018736426.1"
/db_xref="GeneID:30037085"
/translation="MYRAIRSFDTGGRGIGSISLKLRRQNKTPLLRVSFEQSMGIGDS
TYVQHEFPVRLVRVDERKAIEEPQCPPPDLYLELPNALSEINRVIDKFKALGTHVRIR
VNKLGHISFEVHGDGLRVESTFKYGKVVLAVDDENNTTQPPELEQDPYSNEPANDPSV
TVMLKDLSNVLRITTVVKRIVVGVCNEYALILYCYLDRDPDKHDDSGDEEVLTYYMSH
FDV"
gene complement(<885684..>886841)
/gene="SER1"
/locus_tag="AWJ20_4903"
/db_xref="GeneID:30037086"
mRNA complement(<885684..>886841)
/gene="SER1"
/locus_tag="AWJ20_4903"
/product="O-phospho-L-serine:2-oxoglutarate transaminase"
/transcript_id="XM_018882006.1"
/db_xref="GeneID:30037086"
CDS complement(885684..886841)
/gene="SER1"
/locus_tag="AWJ20_4903"
/inference="similar to AA sequence:KEGG_Orthology:K00831"
/note="3-phosphoserine aminotransferase; catalyzes the
formation of phosphoserine from 3-phosphohydroxypyruvate,
required for serine and glycine biosynthesis; regulated by
the general control of amino acid biosynthesis mediated by
Gcn4p; protein abundance increases in response to DNA
replication stress; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_function: GO:0004648 -
O-phospho-L-serine:2-oxoglutarate aminotransferase
activity [Evidence IEA,IEA]; GO_function: GO:0004648 -
O-phospho-L-serine:2-oxoglutarate aminotransferase
activity [Evidence IMP,ISS] [PMID 8017107]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0030170 - pyridoxal phosphate binding
[Evidence IEA]; GO_function: GO:0008483 - transaminase
activity [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0006564 - L-serine biosynthetic process [Evidence
IEA,IEA]; GO_process: GO:0006564 - L-serine biosynthetic
process [Evidence IMP,ISS] [PMID 8017107]; GO_process:
GO:0008652 - cellular amino acid biosynthetic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0009113 - purine nucleobase
biosynthetic process [Evidence IMP] [PMID 10509016];
GO_process: GO:0009070 - serine family amino acid
biosynthetic process [Evidence IMP] [PMID 1326413]"
/codon_start=1
/product="O-phospho-L-serine:2-oxoglutarate transaminase"
/protein_id="XP_018736427.1"
/db_xref="GeneID:30037086"
/translation="MVLNKLDRAPPHYFGAGPALLPTEVLQQAAVELIQYQNLGIGLG
EMSHRSSQAIDIINTTKQKVTQLLDVPDTHEVFFAQGGGTGGFAAVAGNLLAAHAHKT
GKKGVANYIVTGSWSQKAAEEAERLGADVNIVVDARKFTPNGKYGVIPAQSDWKFTPN
AEDIAYVYYCDNETVGGVEFHAPPEVPEGVELVADMSSNFLSKPVDVSKFGLIFGGAQ
KNIGIAGISLYIIKKSLLQHASLEQLRQLQVPIIPIFLDFPTLVKNNSAYNTLSIFAL
RIVQLNVEHLLAKGGLKAQGEESKRKAAKIYALLDKHPEIYKSPVAPTARSNMNIVFT
IPGDGKEETFIKEAAIWGLTGLKGHRSVGGIRVSNCKSTGDVLPPAAGAPP"
gene <888611..>889315
/locus_tag="AWJ20_4904"
/db_xref="GeneID:30037087"
mRNA <888611..>889315
/locus_tag="AWJ20_4904"
/product="hypothetical protein"
/transcript_id="XM_018882007.1"
/db_xref="GeneID:30037087"
CDS 888611..889315
/locus_tag="AWJ20_4904"
/inference="similar to AA sequence:KEGG_Orthology:K13119"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736428.1"
/db_xref="GeneID:30037087"
/translation="MESNGGLVSLKELRRRKEEAKRLAAGGVNVPTEEIKPAEEATNS
QDRSTSSPAASTDSGERKRELTSDNSTITTTKSRFKKRKQVFSKLSFANDEDEEDDNE
RDNGPSRPVSKLKRDPTIDTSNLKTKKQILKDRELLNKEKNSEAQKHAIIREQVVNFP
FVYYDGSETASFVEIKKGDPVWMILEKTRKGFKKFHRGTVDDIMLVKHNVIIPHVSPP
PLLLHHTSIYSLNLFY"
gene complement(<889958..>890764)
/gene="BUD23"
/locus_tag="AWJ20_4905"
/db_xref="GeneID:30037088"
mRNA complement(<889958..>890764)
/gene="BUD23"
/locus_tag="AWJ20_4905"
/product="Bud23p"
/transcript_id="XM_018882008.1"
/db_xref="GeneID:30037088"
CDS complement(889958..890764)
/gene="BUD23"
/locus_tag="AWJ20_4905"
/inference="similar to AA sequence:KEGG_Orthology:K19306"
/note="Methyltransferase; methylates residue G1575 of 18S
rRNA; required for rRNA processing and nuclear export of
40S ribosomal subunits independently of methylation
activity; diploid mutant displays random budding pattern;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005730 - nucleolus [Evidence
IDA] [PMID 18332120]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0008757 -
S-adenosylmethionine-dependent methyltransferase activity
[Evidence ISS] [PMID 9873020]; GO_function: GO:0008168 -
methyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0016435 - rRNA (guanine) methyltransferase
activity [Evidence IMP] [PMID 18332120]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0000282 - cellular bud site selection
[Evidence IMP] [PMID 11452010]; GO_process: GO:0000447 -
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
18332120]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0032259 - methylation
[Evidence IEA]; GO_process: GO:0070476 - rRNA
(guanine-N7)-methylation [Evidence IMP] [PMID 18332120];
GO_process: GO:0000056 - ribosomal small subunit export
from nucleus [Evidence IMP] [PMID 18332120]"
/codon_start=1
/product="Bud23p"
/protein_id="XP_018736429.1"
/db_xref="GeneID:30037088"
/translation="MSRPEYTAPPEVFYNDDESKKYTTSTRVQHIQAEMTLRALELLN
VPPSQHILDIGCGSGLSGEILSEEGHSWIGMDISTSMIANAVDREVEGDLFLADMGSG
IPFRAGSFDAAISISAIQWLCNADTSTANPKKRLMTFFQTLYASLKRGGKVVCQFYPS
NDKQTDAILNAAKMTGFAGGLVVDDAESKKNKKYYLVLAAGQTDADQEINLDGVKMDA
SAMVTSKKRRNKKDESRKDYIMRKKELMRKRGKTIAKDSKFTARKRGPKF"
gene complement(<892332..>893027)
/gene="VMA4"
/locus_tag="AWJ20_4906"
/db_xref="GeneID:30037089"
mRNA complement(<892332..>893027)
/gene="VMA4"
/locus_tag="AWJ20_4906"
/product="H(+)-transporting V1 sector ATPase subunit E"
/transcript_id="XM_018882009.1"
/db_xref="GeneID:30037089"
CDS complement(892332..893027)
/gene="VMA4"
/locus_tag="AWJ20_4906"
/inference="similar to AA sequence:KEGG_Orthology:K02150"
/note="Subunit E of the V1 domain of the vacuolar
H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton
pump found throughout the endomembrane system; V1 domain
has eight subunits; required for the V1 domain to assemble
onto the vacuolar membrane; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence TAS]
[PMID 11717306]; GO_component: GO:0000329 - fungal-type
vacuole membrane [Evidence IDA] [PMID 23708375];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0033178 -
proton-transporting two-sector ATPase complex, catalytic
domain [Evidence IEA]; GO_component: GO:0005774 - vacuolar
membrane [Evidence IEA]; GO_component: GO:0000221 -
vacuolar proton-transporting V-type ATPase, V1 domain
[Evidence IDA] [PMID 16774922]; GO_component: GO:0005773 -
vacuole [Evidence IEA]; GO_function: GO:0046961 -
proton-transporting ATPase activity, rotational mechanism
[Evidence IEA]; GO_function: GO:0046961 -
proton-transporting ATPase activity, rotational mechanism
[Evidence TAS] [PMID 10224039]; GO_function: GO:0046961 -
proton-transporting ATPase activity, rotational mechanism
[Evidence TAS] [PMID 9442887]; GO_process: GO:0015991 -
ATP hydrolysis coupled proton transport [Evidence IEA];
GO_process: GO:0006811 - ion transport [Evidence IEA];
GO_process: GO:0015992 - proton transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA];
GO_process: GO:0007035 - vacuolar acidification [Evidence
TAS] [PMID 10224039]; GO_process: GO:0007035 - vacuolar
acidification [Evidence TAS] [PMID 9442887]"
/codon_start=1
/product="H(+)-transporting V1 sector ATPase subunit E"
/protein_id="XP_018736430.1"
/db_xref="GeneID:30037089"
/translation="MPANQALSDDQVAGELQKMVEFIKKEADEKAKEIELKANEEYEI
DKATVVRSETASIDHLYERKYKQSELSQQIAKSTIANQIRLKVLRAKEDALSEILEEA
AQKLKTVANDKAKYETVLDGLIQEGLFALMDDKISLRVREADVGLVEKAIPSAIKAFE
EKTSHTGTQVSIDKDNFLPKDSAGGVIIVNSTGKIDVDNTLEERLKLLSETSLPQIRL
ALFGASASRKHFD"
gene <894757..>895827
/locus_tag="AWJ20_4907"
/db_xref="GeneID:30037090"
mRNA <894757..>895827
/locus_tag="AWJ20_4907"
/product="hypothetical protein"
/transcript_id="XM_018882010.1"
/db_xref="GeneID:30037090"
CDS 894757..895827
/locus_tag="AWJ20_4907"
/inference="similar to AA sequence:KEGG_Orthology:K14715"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736431.1"
/db_xref="GeneID:30037090"
/translation="MITSLGIGILVGTSLIVIIPEGADAMYSIGLAQGTGSAVDAFGG
DKGMTGDGTEMDSGAGGGGGGGLDSRKSIGLALIAGYLLMYLIDKLPSSWDGLWSLIR
HRRGGRNRFNMGIDMSSLRFTSLGSGGGTGGVGGGVGGGRSSDSIERGLGSGGSGGSG
GSGGHGLSGGGLGGGSGGGVGGGGGEVKSTSTAIGLIIHAIADGVALGASVSTESSAL
ETIIFLAIMIHKAPASFGLTTVLIGSGVDLDKVKRDLIAFAAAAPVGAILTYFLISIL
GSNDSELIQWWTGVLLVFSGGTFLYVAVHAMSELHDVNSPINSLEYSSSSSPTPIEPS
MDLLLSSVGMLVPLVTFFVPDV"
gene <899325..>900770
/gene="CSR1"
/locus_tag="AWJ20_4908"
/db_xref="GeneID:30037091"
mRNA <899325..>900770
/gene="CSR1"
/locus_tag="AWJ20_4908"
/product="Csr1p"
/transcript_id="XM_018882011.1"
/db_xref="GeneID:30037091"
CDS 899325..900770
/gene="CSR1"
/locus_tag="AWJ20_4908"
/note="Phosphatidylinositol transfer protein; has a
potential role in regulating lipid and fatty acid
metabolism under heme-depleted conditions; interacts
specifically with thioredoxin peroxidase; may have a role
in oxidative stress resistance; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
12869188]; GO_component: GO:0005768 - endosome [Evidence
IEA,IEA]; GO_component: GO:0005768 - endosome [Evidence
IDA] [PMID 10848624]; GO_component: GO:0005768 - endosome
[Evidence IDA] [PMID 12869188]; GO_component: GO:0005811 -
lipid particle [Evidence IDA] [PMID 17803462];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005628 -
prospore membrane [Evidence IDA] [PMID 24390141];
GO_function: GO:0008525 - phosphatidylcholine transporter
activity [Evidence IDA] [PMID 10848624]; GO_function:
GO:0008526 - phosphatidylinositol transporter activity
[Evidence IDA] [PMID 10848624]; GO_process: GO:0043001 -
Golgi to plasma membrane protein transport [Evidence IGI]
[PMID 10848624]; GO_process: GO:0006893 - Golgi to plasma
membrane transport [Evidence IGI] [PMID 16126894];
GO_process: GO:0016042 - lipid catabolic process [Evidence
IEA]; GO_process: GO:0006629 - lipid metabolic process
[Evidence IEA]; GO_process: GO:0006869 - lipid transport
[Evidence IEA]; GO_process: GO:0045717 - negative
regulation of fatty acid biosynthetic process [Evidence
IDA,IGI,IMP] [PMID 17803462]; GO_process: GO:1901352 -
negative regulation of phosphatidylglycerol biosynthetic
process [Evidence IGI] [PMID 12869188]; GO_process:
GO:0046488 - phosphatidylinositol metabolic process
[Evidence IGI] [PMID 16262726]; GO_process: GO:0009395 -
phospholipid catabolic process [Evidence IEA]; GO_process:
GO:0015914 - phospholipid transport [Evidence IDA] [PMID
10848624]; GO_process: GO:2001247 - positive regulation of
phosphatidylcholine biosynthetic process [Evidence IGI]
[PMID 12869188]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Csr1p"
/protein_id="XP_018736432.1"
/db_xref="GeneID:30037091"
/translation="MTPTTSYPELNMTSSNVVRTFTASSRMSHISFGSSNSDLTPSNR
SILSESSDSTPLPEDYVIKVKQMYAHILVYTGDAPKSTIPGLMASTTKNFDHRAQNVL
KSHLYKISPDKFKTTYRNVVRAENFDIILLRYLKKRKFNVLKALALFADFMVWRVKFA
IDELNERGDEGALASGDEGFILQYKLGKAVFNQGRDKAGRPLVIVRVQRHNPKAQSDA
AMNRFIAKVFEDAKLLLLGYNESGAAIFDLTDFTISNMDYSAVKMINSCYKFYVGLLG
HVYIYNAPSIFLTIWKAIKVWLDPSLVARVTFTKNLSKHITADNLPPDMEGTQPHIYE
YKQVVPGENRKMKDLETIDRLQTTRGELFDLLSDLTVLWIKAPENTQLTHNLAKQRAL
VLDDLRRNYYQLDPYIRGRTVYDRDGTFGAIVPELTEGYAESEAAGIVLSQSASSIVT
AVNNPPPDSSPRASHSPKHMHTKVSLVPLIV"
gene <903030..>905768
/gene="APL5"
/locus_tag="AWJ20_4909"
/db_xref="GeneID:30037092"
mRNA <903030..>905768
/gene="APL5"
/locus_tag="AWJ20_4909"
/product="Apl5p"
/transcript_id="XM_018882012.1"
/db_xref="GeneID:30037092"
CDS 903030..905768
/gene="APL5"
/locus_tag="AWJ20_4909"
/inference="similar to AA sequence:KEGG_Orthology:K12396"
/note="Delta adaptin-like subunit of the clathrin
associated protein complex; functions in transport of
alkaline phosphatase to the vacuole via the alternate
pathway; suppressor of loss of casein kinase 1 function;
the clathrin associated protein complex is also known as
AP-3; GO_component: GO:0030123 - AP-3 adaptor complex
[Evidence IMP] [PMID 9250663]; GO_component: GO:0005794 -
Golgi apparatus [Evidence IEA,IEA,IEA]; GO_component:
GO:0030665 - clathrin-coated vesicle membrane [Evidence
IEA]; GO_component: GO:0031410 - cytoplasmic vesicle
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0030117 - membrane coat
[Evidence IEA]; GO_function: GO:0005515 - protein binding
[Evidence IPI] [PMID 10559961]; GO_function: GO:0008565 -
protein transporter activity [Evidence IEA]; GO_process:
GO:0006896 - Golgi to vacuole transport [Evidence IMP]
[PMID 9335339]; GO_process: GO:0006886 - intracellular
protein transport [Evidence IEA]; GO_process: GO:0006623 -
protein targeting to vacuole [Evidence IMP] [PMID
17895371]; GO_process: GO:0015031 - protein transport
[Evidence IEA,IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]; GO_process: GO:0016192 - vesicle-mediated
transport [Evidence IEA]"
/codon_start=1
/product="Apl5p"
/protein_id="XP_018736433.1"
/db_xref="GeneID:30037092"
/translation="MAIMKLTYLEMYGNDMSWASFHVLEVMSSAKFQQKRVGYLAAAQ
SFRANTDVLMLTTNLLKKDLGSANPLEVSTSLSAAASIVTPSLAHDLSPDILKMLNHS
RPYIRKKAVLAMYKVFLQYPEALPGAFPRLKDKLEDADPSVVSATVNVICELAKQTSG
TGSEITKTYLSLAPQLYELLTTSKNNWMLIKILKLFSALAPTEPRLKPKLLPPIIQLM
RTTTAMSLLYECINCIVSGDMLDEDDYEVAELCVSKLRTFLEEHDQNLKYVGLLALGK
IVVKHPQFVINLQDVILECADDKDLTIRDCVLDLIPGIVTENNIYHIVNKLMDQLRAS
RGILSPGSAGVAAGAGGTSAEDNKNNSALDNHNSRIIESIISVCSKDTYSLVPDFEWY
TTILVELVQLGTDNVGWRIGDQLRNIAIRVKSMRGVIVEASVRLLMNYQLMTHMPSVV
KFVVWIVGEYASLVPNVHELLTQLITSRYGDEEAELGYMANSGISGTFGMFTTFGSAV
SGGAGGNTSSSHGNGRHGPGSNSPSNAQFGTVEASIQAVVKLYSNWAGQANWPSGFHF
QVLEATNQLITYLEHYSTAISYEVQERAVGFLELVKVAKEAIEQLPDNSETPPLLLTL
AIPSMFNGYELNPVAQNSQRKIPLPEDLDLDTEIYPAYALSDIEDDYEIEDEASTLVD
GTTILIEDTPLTEQDLERKRLERLERQRDDPFYIPISSGNQTPLENSRSNTPTPGIAI
ANAGGSGSQPSSYTPGSHISSASQPSKFMPPPRRTKVEIIQDEKIDGVDDVDETTTTT
VKTKSKTSKSRSKPLFKIESRLTKLDLNPETSTTTDQAATLEVQKLRQQLLNESSSKP
AASSDEILVTHKKIKKKKPKSTTGEAGEATPKKKKKKKVTSSEPPAAAEPAPETMY"
gene <906218..>907750
/gene="RAD52"
/locus_tag="AWJ20_4910"
/db_xref="GeneID:30037094"
mRNA <906218..>907750
/gene="RAD52"
/locus_tag="AWJ20_4910"
/product="recombinase RAD52"
/transcript_id="XM_018882014.1"
/db_xref="GeneID:30037094"
CDS 906218..907750
/gene="RAD52"
/locus_tag="AWJ20_4910"
/inference="similar to AA sequence:KEGG_Orthology:K10873"
/note="Protein that stimulates strand exchange; stimulates
strand exchange by facilitating Rad51p binding to
single-stranded DNA; anneals complementary single-stranded
DNA; involved in the repair of double-strand breaks in DNA
during vegetative growth and meiosis and UV induced sister
chromatid recombination; GO_component: GO:0000228 -
nuclear chromosome [Evidence IDA] [PMID 12766777];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11459964]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 22842922]; GO_function: GO:0000150 -
recombinase activity [Evidence IDA] [PMID 15205482];
GO_process: GO:0006277 - DNA amplification [Evidence IMP]
[PMID 23271978]; GO_process: GO:0000730 - DNA recombinase
assembly [Evidence IDA] [PMID 11459983]; GO_process:
GO:0006310 - DNA recombination [Evidence IEA,IEA];
GO_process: GO:0006281 - DNA repair [Evidence IEA,IEA];
GO_process: GO:0000733 - DNA strand renaturation [Evidence
IDA] [PMID 9619627]; GO_process: GO:0006974 - cellular
response to DNA damage stimulus [Evidence IEA];
GO_process: GO:0000727 - double-strand break repair via
break-induced replication [Evidence IMP] [PMID 17096599];
GO_process: GO:0000724 - double-strand break repair via
homologous recombination [Evidence IMP] [PMID 17096599];
GO_process: GO:0000724 - double-strand break repair via
homologous recombination [Evidence IMP] [PMID 24190138];
GO_process: GO:0045002 - double-strand break repair via
single-strand annealing [Evidence IGI] [PMID 11606529];
GO_process: GO:0000709 - meiotic joint molecule formation
[Evidence IGI,IMP] [PMID 18313389]; GO_process: GO:0006301
- postreplication repair [Evidence IMP] [PMID 7038396];
GO_process: GO:0000722 - telomere maintenance via
recombination [Evidence IMP] [PMID 11238918]"
/codon_start=1
/product="recombinase RAD52"
/protein_id="XP_018736434.1"
/db_xref="GeneID:30037094"
/translation="MPNYGDQHLSNGSRYGTNGGGGYLPNNNRSWSNGNASGSGTGSS
GNGGAAVNGTNFTIKESNHIRQQLGKQLGPEYISHRPGGGGLKVPYLEGWKVINLANE
VFGFNGWSSEIINHEIDYCDEIRDGRFNLGMSAKIRVTLRDGTYREDVGYGQVENARS
KGMAFDKCKKEATTDGLKRALRLFGNALGNCLYDSSYTRNISRVKVSMPPFDESQLIR
RPEIVTMQKAAAAAKAAREAAEAAELEELAALNTSTKPSIKSEASTVAASSTPQNGLA
AKGTNLTAVGTGNNGATSRIANNSGSGQPSLPSLPTKAFAKPDGSGVDSSSTAVSGSL
DFMPAGFLDSDDMFDDHEFAEIDGLPYIAPREEPVPAPARVPLNIKADTSIDTTANIT
ANTTADLTSNTTANTTANITNESGTTTNKPNKESEDHTASPVQFFSARAALAVQKDMI
PENSQFDPSFQSPSLRRTVPNKSVPIKKSSVNPLSQDPQPPTLASDRTPEPDSRKRMR
LD"
gene complement(<907976..>908749)
/gene="GCD7"
/locus_tag="AWJ20_4911"
/db_xref="GeneID:30037095"
mRNA complement(<907976..>908749)
/gene="GCD7"
/locus_tag="AWJ20_4911"
/product="translation initiation factor eIF2B subunit
beta"
/transcript_id="XM_018882015.1"
/db_xref="GeneID:30037095"
CDS complement(907976..908749)
/gene="GCD7"
/locus_tag="AWJ20_4911"
/inference="similar to AA sequence:KEGG_Orthology:K03754"
/note="Beta subunit of the translation initiation factor
eIF2B; the guanine-nucleotide exchange factor for eIF2;
activity subsequently regulated by phosphorylated eIF2;
first identified as a negative regulator of GCN4
expression; GO_component: GO:0005851 - eukaryotic
translation initiation factor 2B complex [Evidence
IDA,IGI,IPI] [PMID 8336705]; GO_component: GO:0005851 -
eukaryotic translation initiation factor 2B complex
[Evidence IMP] [PMID 8441423]; GO_component: GO:0005851 -
eukaryotic translation initiation factor 2B complex
[Evidence IDA,IPI] [PMID 8506384]; GO_component:
GO:0032045 - guanyl-nucleotide exchange factor complex
[Evidence IPI] [PMID 8336705]; GO_component: GO:0032045 -
guanyl-nucleotide exchange factor complex [Evidence IDA]
[PMID 8506384]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0030234 - enzyme regulator activity [Evidence IGI]
[PMID 8164676]; GO_function: GO:0030234 - enzyme regulator
activity [Evidence IGI,IPI] [PMID 8887689]; GO_function:
GO:0030234 - enzyme regulator activity [Evidence IMP]
[PMID 9032257]; GO_function: GO:0005085 -
guanyl-nucleotide exchange factor activity [Evidence IPI]
[PMID 8336705]; GO_function: GO:0005085 -
guanyl-nucleotide exchange factor activity [Evidence IDA]
[PMID 8506384]; GO_function: GO:0003743 - translation
initiation factor activity [Evidence IEA]; GO_function:
GO:0003743 - translation initiation factor activity
[Evidence IGI,IPI] [PMID 8336705]; GO_process: GO:0044237
- cellular metabolic process [Evidence IEA]; GO_process:
GO:0006417 - regulation of translation [Evidence IEA];
GO_process: GO:0006446 - regulation of translational
initiation [Evidence IGI] [PMID 8164676]; GO_process:
GO:0006446 - regulation of translational initiation
[Evidence IDA] [PMID 8506384]; GO_process: GO:0006446 -
regulation of translational initiation [Evidence IGI,IPI]
[PMID 8887689]; GO_process: GO:0006446 - regulation of
translational initiation [Evidence IMP] [PMID 9032257];
GO_process: GO:0006412 - translation [Evidence IEA];
GO_process: GO:0006413 - translational initiation
[Evidence IEA]"
/codon_start=1
/product="translation initiation factor eIF2B subunit
beta"
/protein_id="XP_018736435.1"
/db_xref="GeneID:30037095"
/translation="MFGLLSVDPSATSNSPTKALTNEEARKAHRDTKADIIEGIQELI
EEISNIDDSIAGMSVDMIHENEVLLTASPNSKTVLEFLLRASQKRTFTVLVTEGFPNE
VTASHEFALTLSKAGIHTVIIPDTATSAVMSRVGKVIIGTRAVLANGGCVSSSGVALV
CEAAKDYRVPVLAVTGLYKLSPLYPFDIDQLIEVGDTGKVIGFNDTGLMDKIGVMNPV
YDYVAPEHIDIYVTNIGGFSPSFTYRIILDHYSNQDVSL"
gene <909623..>910453
/locus_tag="AWJ20_4912"
/db_xref="GeneID:30037096"
mRNA <909623..>910453
/locus_tag="AWJ20_4912"
/product="hypothetical protein"
/transcript_id="XM_018882016.1"
/db_xref="GeneID:30037096"
CDS 909623..910453
/locus_tag="AWJ20_4912"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736436.1"
/db_xref="GeneID:30037096"
/translation="MTYFFVKAIPGVQDFGVHVASLASLTVADLISQINSRLPPAVAK
STYVSMQNGSIISQNRNQKLSEVFPDDPEYLLLRLNGCLCGGKGGFGSMLRDMGKKKR
KKGPRREEEMDSFKTLDGRSMKSIRQAKELLAHLDKLPEMEKQKIEKKRAKLLSIIDS
ANRATNSASSSKFSDTDYLETCEKLVSDVRQAVMNHSEESLSDSASVSSETSKSSKSS
VTDSEISSKSTSLKGKEKTTQKTPNIQITSSEEVVVATSRPKAMNFFEDEDYDEDDDE
"
gene <911509..>912012
/gene="JEN1"
/locus_tag="AWJ20_4913"
/db_xref="GeneID:30037097"
mRNA <911509..>912012
/gene="JEN1"
/locus_tag="AWJ20_4913"
/product="Jen1p"
/transcript_id="XM_018882017.1"
/db_xref="GeneID:30037097"
CDS 911509..912012
/gene="JEN1"
/locus_tag="AWJ20_4913"
/inference="similar to AA sequence:KEGG_Orthology:K08178"
/note="Monocarboxylate/proton symporter of the plasma
membrane; transport activity is dependent on the pH
gradient across the membrane; mediates high-affinity
uptake of carbon sources lactate, pyuvate, and acetate,
and also of the micronutrient selenite, whose structure
mimics that of monocarboxylates; expression and
localization are tightly regulated, with transcription
repression, mRNA degradation, and protein endocytosis and
degradation all occurring in the presence of glucose;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 17710650];
GO_function: GO:0015144 - carbohydrate transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0015355 - secondary active monocarboxylate
transmembrane transporter activity [Evidence IMP] [PMID
17710650]; GO_function: GO:0097079 - selenite:proton
symporter activity [Evidence IMP] [PMID 20861301];
GO_function: GO:0022857 - transmembrane transporter
activity [Evidence IEA]; GO_process: GO:0034219 -
carbohydrate transmembrane transport [Evidence IEA];
GO_process: GO:0035879 - plasma membrane lactate transport
[Evidence IMP] [PMID 10198029]; GO_process: GO:0035879 -
plasma membrane lactate transport [Evidence IMP] [PMID
17710650]; GO_process: GO:0006849 - plasma membrane
pyruvate transport [Evidence IMP] [PMID 17710650];
GO_process: GO:0097080 - plasma membrane selenite
transport [Evidence IMP] [PMID 20861301]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Jen1p"
/protein_id="XP_018736437.1"
/db_xref="GeneID:30037097"
/translation="MSSQSYSPYVEDEKPDLSVGALVRYLASRPSTLFTLPESDTKRL
NPIPPLRAMKLRHWNWYMMGFLAWTVDAMDFFVVSASASAIAESLNVTIVKITWGMTL
VLMFRSVGAVIFGTISDTWGRKWSYIACCLCFVALEIGMGFITSYKQFLAVRALFGIS
MGGKSSR"
gene <912067..>913065
/gene="JEN1"
/locus_tag="AWJ20_4914"
/db_xref="GeneID:30037098"
mRNA <912067..>913065
/gene="JEN1"
/locus_tag="AWJ20_4914"
/product="Jen1p"
/transcript_id="XM_018882018.1"
/db_xref="GeneID:30037098"
CDS 912067..913065
/gene="JEN1"
/locus_tag="AWJ20_4914"
/inference="similar to AA sequence:KEGG_Orthology:K08178"
/note="Monocarboxylate/proton symporter of the plasma
membrane; transport activity is dependent on the pH
gradient across the membrane; mediates high-affinity
uptake of carbon sources lactate, pyuvate, and acetate,
and also of the micronutrient selenite, whose structure
mimics that of monocarboxylates; expression and
localization are tightly regulated, with transcription
repression, mRNA degradation, and protein endocytosis and
degradation all occurring in the presence of glucose;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 17710650];
GO_function: GO:0015144 - carbohydrate transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0015355 - secondary active monocarboxylate
transmembrane transporter activity [Evidence IMP] [PMID
17710650]; GO_function: GO:0097079 - selenite:proton
symporter activity [Evidence IMP] [PMID 20861301];
GO_function: GO:0022857 - transmembrane transporter
activity [Evidence IEA]; GO_process: GO:0034219 -
carbohydrate transmembrane transport [Evidence IEA];
GO_process: GO:0035879 - plasma membrane lactate transport
[Evidence IMP] [PMID 10198029]; GO_process: GO:0035879 -
plasma membrane lactate transport [Evidence IMP] [PMID
17710650]; GO_process: GO:0006849 - plasma membrane
pyruvate transport [Evidence IMP] [PMID 17710650];
GO_process: GO:0097080 - plasma membrane selenite
transport [Evidence IMP] [PMID 20861301]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Jen1p"
/protein_id="XP_018736438.1"
/db_xref="GeneID:30037098"
/translation="MYGTAAATSLENIPPESISMMSGLFLPGYNLGYIFAIIFYRAFQ
YTHGGEGWRSLCWFTAGPAALLAIWRLCFPEGEYFSKLKEKREFEKLNAVGRWNDAKV
AIANYWVVFVYLIFLMSGMNFMSHGTMDLYPTLLVTQAKLGTDAKTVVMVVSNLGAIA
GGLIFGQLSEILGRRLCISVCCLLGGAFVYPAFLGANENTLMAGSTLLMAAVFGAWGQ
ITIHLFELSPPRYRTLAAGLAYQLGNLASSASATIEATIGARFPIGDKPGVYNYGLVM
CIFSGAVFGYVLIVIFLGPERFHRDLYEQVRTDDEKIYTLDDEKAETHHIDSVGRV"
gene <913920..>914177
/locus_tag="AWJ20_4915"
/db_xref="GeneID:30037099"
mRNA <913920..>914177
/locus_tag="AWJ20_4915"
/product="hypothetical protein"
/transcript_id="XM_018882019.1"
/db_xref="GeneID:30037099"
CDS 913920..914177
/locus_tag="AWJ20_4915"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736439.1"
/db_xref="GeneID:30037099"
/translation="MDQDVMVPWVLQNKVNKKKNQLKEAAAEPETAPEPPKPEEPPVV
WNRYYHLYKQGELEEDVVTAKGRVVESGYERDNWWAIISKE"
gene complement(<914375..>915817)
/gene="PRP8"
/locus_tag="AWJ20_4916"
/db_xref="GeneID:30037100"
mRNA complement(<914375..>915817)
/gene="PRP8"
/locus_tag="AWJ20_4916"
/product="U4/U6-U5 snRNP complex component PRP8"
/transcript_id="XM_018882020.1"
/db_xref="GeneID:30037100"
CDS complement(914375..915817)
/gene="PRP8"
/locus_tag="AWJ20_4916"
/inference="similar to AA sequence:KEGG_Orthology:K12856"
/note="Component of U4/U6-U5 snRNP complex; involved in
second catalytic step of splicing; participates in
spliceosomal assembly through its interaction with U1
snRNA; largest and most evolutionarily conserved protein
of the spliceosome; mutations in its human ortholog,
PRPF8, cause Retinitis pigmentosa and missplicing in
Myelodysplastic syndrome; GO_component: GO:0046540 - U4/U6
x U5 tri-snRNP complex [Evidence IDA] [PMID 10377396];
GO_component: GO:0046540 - U4/U6 x U5 tri-snRNP complex
[Evidence IDA] [PMID 10449419]; GO_component: GO:0046540 -
U4/U6 x U5 tri-snRNP complex [Evidence IDA] [PMID
17934474]; GO_component: GO:0005682 - U5 snRNP [Evidence
IDA] [PMID 11720284]; GO_component: GO:0005682 - U5 snRNP
[Evidence IDA] [PMID 11720285]; GO_component: GO:0005682 -
U5 snRNP [Evidence IDA] [PMID 17934474]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 17934474];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22842922]; GO_component: GO:0030529 - ribonucleoprotein
complex [Evidence IEA]; GO_component: GO:0005681 -
spliceosomal complex [Evidence IEA,IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IEA,IEA]; GO_function:
GO:0030619 - U1 snRNA binding [Evidence IDA] [PMID
23393194]; GO_function: GO:0030620 - U2 snRNA binding
[Evidence IDA] [PMID 23393194]; GO_function: GO:0030623 -
U5 snRNA binding [Evidence IEA]; GO_function: GO:0030623 -
U5 snRNA binding [Evidence IDA] [PMID 23393194];
GO_function: GO:0030623 - U5 snRNA binding [Evidence IDA]
[PMID 9848662]; GO_function: GO:0017070 - U6 snRNA binding
[Evidence IEA]; GO_function: GO:0017070 - U6 snRNA binding
[Evidence IDA] [PMID 23393194]; GO_function: GO:0003729 -
mRNA binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0003676 - nucleic acid binding [Evidence IEA];
GO_function: GO:0097157 - pre-mRNA intronic binding
[Evidence IDA] [PMID 23393194]; GO_function: GO:0000386 -
second spliceosomal transesterification activity [Evidence
IMP] [PMID 17626844]; GO_process: GO:0008380 - RNA
splicing [Evidence IEA]; GO_process: GO:0000350 -
generation of catalytic spliceosome for second
transesterification step [Evidence IMP] [PMID 22408182];
GO_process: GO:0000389 - mRNA 3'-splice site recognition
[Evidence IMP] [PMID 10628969]; GO_process: GO:0006397 -
mRNA processing [Evidence IEA]; GO_process: GO:0000398 -
mRNA splicing, via spliceosome [Evidence IEA]; GO_process:
GO:0000244 - spliceosomal tri-snRNP complex assembly
[Evidence IMP] [PMID 17934474]; GO_process: GO:0000244 -
spliceosomal tri-snRNP complex assembly [Evidence IDA]
[PMID 23393194]"
/codon_start=1
/product="U4/U6-U5 snRNP complex component PRP8"
/protein_id="XP_018736440.1"
/db_xref="GeneID:30037100"
/translation="MLDPLEVHMLDFPNISIRPSELQLPFQDAMKIEKLADTILKATE
PKMLLYNLYDDWLIDNKTEPGTAFSRLILILRALHVNQEKAKLILRPDRSVLTEEHHL
WPSFTTEQWINVENQLSDLILNDYGKKNNVNVASLTQHEIRDLILGQEIKAPSLKRQE
MADIDQQRQDQQSQLTALKTKTQNVHGDEMVVVTTSNYEQESFSSKTDWRNRAIASTS
INLRTKHIYVSSEDIRDDSQNYTYILPKNILKKFTQISDLRAQVGGFLYGVSPPDNPQ
IKEIRVIALVPQVGNANSVQLPLKLPSTGGSEPLLKGLEPLGWIHTQAQETPVLSAID
VTTQAKIMDANSGAAGGWDSNAITMTVSFTPGSVTLSAFSLKPEGYEWGSRNRDIAAI
NPLGYSTKFGEKCQLLLSDRINGYFLVPEDSVWNYAFMGAAWSANAAYELKIDVPLPY
YHELHRPIHFTSFNDLEDNVIELDKQDVFA"
gene complement(<916027..>921720)
/gene="PRP8"
/locus_tag="AWJ20_4917"
/db_xref="GeneID:30037101"
mRNA complement(<916027..>921720)
/gene="PRP8"
/locus_tag="AWJ20_4917"
/product="U4/U6-U5 snRNP complex component PRP8"
/transcript_id="XM_018882021.1"
/db_xref="GeneID:30037101"
CDS complement(916027..921720)
/gene="PRP8"
/locus_tag="AWJ20_4917"
/inference="similar to AA sequence:KEGG_Orthology:K12856"
/note="Component of U4/U6-U5 snRNP complex; involved in
second catalytic step of splicing; participates in
spliceosomal assembly through its interaction with U1
snRNA; largest and most evolutionarily conserved protein
of the spliceosome; mutations in its human ortholog,
PRPF8, cause Retinitis pigmentosa and missplicing in
Myelodysplastic syndrome; GO_component: GO:0046540 - U4/U6
x U5 tri-snRNP complex [Evidence IDA] [PMID 10377396];
GO_component: GO:0046540 - U4/U6 x U5 tri-snRNP complex
[Evidence IDA] [PMID 10449419]; GO_component: GO:0046540 -
U4/U6 x U5 tri-snRNP complex [Evidence IDA] [PMID
17934474]; GO_component: GO:0005682 - U5 snRNP [Evidence
IDA] [PMID 11720284]; GO_component: GO:0005682 - U5 snRNP
[Evidence IDA] [PMID 11720285]; GO_component: GO:0005682 -
U5 snRNP [Evidence IDA] [PMID 17934474]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 17934474];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22842922]; GO_component: GO:0030529 - ribonucleoprotein
complex [Evidence IEA]; GO_component: GO:0005681 -
spliceosomal complex [Evidence IEA,IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IEA,IEA]; GO_function:
GO:0030619 - U1 snRNA binding [Evidence IDA] [PMID
23393194]; GO_function: GO:0030620 - U2 snRNA binding
[Evidence IDA] [PMID 23393194]; GO_function: GO:0030623 -
U5 snRNA binding [Evidence IEA]; GO_function: GO:0030623 -
U5 snRNA binding [Evidence IDA] [PMID 23393194];
GO_function: GO:0030623 - U5 snRNA binding [Evidence IDA]
[PMID 9848662]; GO_function: GO:0017070 - U6 snRNA binding
[Evidence IEA]; GO_function: GO:0017070 - U6 snRNA binding
[Evidence IDA] [PMID 23393194]; GO_function: GO:0003729 -
mRNA binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0003676 - nucleic acid binding [Evidence IEA];
GO_function: GO:0097157 - pre-mRNA intronic binding
[Evidence IDA] [PMID 23393194]; GO_function: GO:0000386 -
second spliceosomal transesterification activity [Evidence
IMP] [PMID 17626844]; GO_process: GO:0008380 - RNA
splicing [Evidence IEA]; GO_process: GO:0000350 -
generation of catalytic spliceosome for second
transesterification step [Evidence IMP] [PMID 22408182];
GO_process: GO:0000389 - mRNA 3'-splice site recognition
[Evidence IMP] [PMID 10628969]; GO_process: GO:0006397 -
mRNA processing [Evidence IEA]; GO_process: GO:0000398 -
mRNA splicing, via spliceosome [Evidence IEA]; GO_process:
GO:0000244 - spliceosomal tri-snRNP complex assembly
[Evidence IMP] [PMID 17934474]; GO_process: GO:0000244 -
spliceosomal tri-snRNP complex assembly [Evidence IDA]
[PMID 23393194]"
/codon_start=1
/product="U4/U6-U5 snRNP complex component PRP8"
/protein_id="XP_018736441.1"
/db_xref="GeneID:30037101"
/translation="MSGRNPGAPPGFPGAGKRPNGGPPPPPPPPGGAGVKRKPGTGGL
APPPPPPPSGGKRRTANGSHPPPPPPPPSRPPPAEKREEERRLRALERKRKWQQLQKQ
KYGAKQGKATFVHSTKVDMPPEHLRKIIRDHGDLSTKRVAADKRSYLGALKFIPHAVV
KLMENMPQPWEQVREVKVLYHITGAITFVNEVPRVIEPVYTAQWATMWTMMRREKRDR
RHFKRMRFPPFDDEEPPISWSENIEIAEPGDAIQMELDQEEDSPVYEWFYDHRPLIED
PKYVPGPAYKKWNLELPQMANLYRLASPLVADIATRNDLYLFDKKSFFTAKALNTAIP
GGPKFEPLYKDVDEDNEDFGEFNAIDRIIFRNPIRTEYKIAFPHLYNSLPRSVQIDWY
HDPQDVHIKTEDPDLPAYYYDPAINPITARRREIDEQEADDLILDDDLEEEEEEEEEE
EKEGEQRSTGFTLGDDFETFLENVPLTTDNTSHAIALWWAPFPFNRRSGTMVRAQDVA
LVKNWYVQHAPDGNPVKVRVSYQKLLKTYVLNALKTKPPSNQKKRYLLKSLKNTKFFQ
QTTIDWVEAGLQLCRQGFTMLNLLIHRKGLTYLHLDYNFNLKPIKTLTTKERKRSRFG
NAFHLMREILRVVKLIVDAHVQYRLGNVDAYQLADGLHYTLNHLGQLTGIYRYKYKVM
HQIRTCKDLKHVIYYRFNSVIGKGPGCGFWQPAWRVWLFFLRGTIPLLERWLGNLLAR
QFEGRHSKAVAKTITKQRVESYYDLELRASVLNDILDMIPEGIKQNKAKTIIQHLSEA
WRCWKANIPWKVPGLPAPIENIILRYVKAKADGWISVAHYNRERIKRGTTVEKTVAKK
NLGRLTRLWLKSEQERQHNFSKDGPYVAPEDAVAIFSTMVHWLESRKFSPIPFPPLSY
KHDTKLLVLALEGLKESYSVKNRLNQSQREELALIEQAYDNPHECLSRIKRFLLTQRV
FKEVGLDMMDYYSHILPVYEIDPLEKITDAYLDQYLWYEADKRHLFPNWIKPSDSEVP
PLLVYKWCQGINNLQDVWDVSEGQCNVMLQTTLSKMAEKIDFTLLNRLLRLIVDSNIA
DYITAKNNVALSYKDMTHVNQYGLIRGLQFSSFVMQYYGLVLDLLLLGLPRASELAGP
PQQPNDFLQFKDEETEVNHPIRLYIRYVDKVHILFRFRATEARDLIQRFLTENPDPNF
ENVVGYNNKKCWPRDSRMRLMRHDVNLGRAVFWEMKNRLPRSLTTFEWEDTFASVYSR
DNPNLLFSMVGFEVRILPKSRVENDIQIRDSVWNLTNEETKERTAYAFLRVTDEDIAK
FNNRIRQILMSSGSTTFTKIANKWNTALISLFTYYREAAVASENLLDTLVKCETKIQT
RVKIGLNSKMPSRFPPAVFYTPKELGGLGMLSASHILIPTSDLRWSKQTDTGITHFRS
GMTHQDDQLIPNIFRYIASWESEFIDSQNVWAEYAMKRQEASQQNRRLTLEDLEDRWD
RGLPRINTLFQKDRHTLAYDKGHRIRTQFRQYSLAKVNPFWWTSQRHDGKLWNLNAYR
TDVIQALGGIETILEHTLFKGTGFDNWEGLFWEKASGFEDSMKFKKLTNAQRSGLNQI
PNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHE
SIIFDICHVLDAEMDTLQIENVNKESIHPRKSYKMNSSAADIVLHSTYKWSVSGPSLL
NDSKDVMDFATANKFWLDIQLRYGDYDSHDISRYTRAKFLDYTSDGISIYPSPTGAMI
GVDLAYNMYDAYGNWFPGLKPLMQQAMSKIMKANPALFVLRERIRKGLQLYQSQPQEA
FLNSNNYSELFGKDVQFFIDDSNVYRVTVHKTFEGNLTTKPINGAIFIFNPRTGK"
gene complement(<926261..>927481)
/gene="STS1"
/locus_tag="AWJ20_4918"
/db_xref="GeneID:30037102"
mRNA complement(<926261..>927481)
/gene="STS1"
/locus_tag="AWJ20_4918"
/product="Sts1p"
/transcript_id="XM_018882022.1"
/db_xref="GeneID:30037102"
CDS complement(926261..927481)
/gene="STS1"
/locus_tag="AWJ20_4918"
/inference="similar to AA sequence:KEGG_Orthology:K11888"
/note="Protein required for localizing proteasomes to the
nucleus; involved in cotranslational protein degradation;
mediates interaction between nuclear import factor Srp1p
and the proteasome; Sts1p and Srp1p couple proteasomes to
nascent polypeptides emerging from the ribosome for
cotranslational degradation; involved in
ubiquitin-mediated protein degradation; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 10913188];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0007059 - chromosome segregation
[Evidence IMP] [PMID 8065366]; GO_process: GO:0071630 -
nucleus-associated proteasomal ubiquitin-dependent protein
catabolic process [Evidence IEA]; GO_process: GO:0071630 -
nucleus-associated proteasomal ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 21075847];
GO_process: GO:0031144 - proteasome localization [Evidence
IEA]; GO_process: GO:0031144 - proteasome localization
[Evidence IMP] [PMID 21075847]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Sts1p"
/protein_id="XP_018736442.1"
/db_xref="GeneID:30037102"
/translation="MVVSAVLPPRTGWGFEFASSSESASQVQDRPGGQFGSPVFGLAG
ENNSGVSAGATGFAGAATAPSTPTLRSGKRKMNSMDRYDSDEDHNIENDNDNDVYDEY
DEHRNFASHSQSRLNGGGPSGTRSVPGSAFRGSNSSVLNSEMDLFSSSFQKRLSTGVS
NRLKAHATGSKVSKRARTHTAMGHPLPIGRILESLDKKNLRSIIENLCQTHPYLVGEI
SNLAPRITVSTAIEHLKNLLSAVYNSLPFKGDQRGDYAYLRVRPAVEEFLSALSDYTS
NFLPPTESQPSNTLAFLDSATDLLHQLPVWSNPVNNHHQWVCYDDIASAWIVAIREAS
KRAGGLGLVHDGWQVKLDKHNEKSDDRLKDAVACIRNELSWLNSNNDEANSTPKQNIF
SIYNPHSQTPSMWT"
gene <928588..>930895
/gene="THS1"
/locus_tag="AWJ20_4919"
/db_xref="GeneID:30037103"
mRNA join(<928588..928674,928835..>930895)
/gene="THS1"
/locus_tag="AWJ20_4919"
/product="threonine--tRNA ligase THS1"
/transcript_id="XM_018882023.1"
/db_xref="GeneID:30037103"
CDS join(928588..928674,928835..930895)
/gene="THS1"
/locus_tag="AWJ20_4919"
/inference="similar to AA sequence:KEGG_Orthology:K01868"
/note="Threonyl-tRNA synthetase; essential cytoplasmic
protein; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IMP] [PMID 2995918]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0004812 -
aminoacyl-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0016876 - ligase activity, forming
aminoacyl-tRNA and related compounds [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_function: GO:0004829 - threonine-tRNA ligase
activity [Evidence IEA,IEA]; GO_function: GO:0004829 -
threonine-tRNA ligase activity [Evidence ISA] [PMID
2995918]; GO_function: GO:0004829 - threonine-tRNA ligase
activity [Evidence IDA] [PMID 8143729]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence IMP] [PMID
10471698]; GO_process: GO:0043039 - tRNA aminoacylation
[Evidence IEA]; GO_process: GO:0006418 - tRNA
aminoacylation for protein translation [Evidence IEA];
GO_process: GO:0006435 - threonyl-tRNA aminoacylation
[Evidence IEA]; GO_process: GO:0006435 - threonyl-tRNA
aminoacylation [Evidence ISA] [PMID 2995918]; GO_process:
GO:0006435 - threonyl-tRNA aminoacylation [Evidence IDA]
[PMID 8143729]; GO_process: GO:0006412 - translation
[Evidence IEA]"
/codon_start=1
/product="threonine--tRNA ligase THS1"
/protein_id="XP_018736443.1"
/db_xref="GeneID:30037103"
/translation="MAAAGVSELIEKVADATIAPKKGPPALYPAEPKFSFIDERLKFF
DELKKAQDEEIAKKERKPIKITLKDGSVREGTAWETSPADIAKEISKSLFEKTVISKV
NGELWDLERPFEEDSKLELLDFDSEDGKKVFWHSSAHVLGEACECHFGAHLCVGPPTD
DGFFYEMSIDNGDRAVSSADFPAVEAVTKRAVKDKQRFERLVVSKENLLKMFAYNKYK
QTLIQSKIPDGTSSTVYRCGPLIDLCRGPHVPHTGRIKSIKLLKNSASYFLGDSANDS
LQRIYGVSFPDKKQLDEHLKFLEEAAKRDHRKIGKDQELFMFHDMSPGSCFWLPHGAR
IYNTLGDFLRTEYRKRGYEEVITPNMYNAKLWETSGHWANYKENMFTFDVEKEKFGLK
PMNCPGHCLMFKARERSYRELPWRVADFGVIHRNEFSGALSGLTRVRRFQQDDAHIFC
RQDQIEAEITGIFEFLKHVYGVFGFDFKMELSTRPEKYVGQLETWNDAEAKLASALDS
WGAKWELNPGDGAFYGPKIDIMISDALKRWHQCATIQLDFQLPDRFGLQFRGADVTDA
AGEVQHYDRPVMIHRALYGSFERMIAILTEHFFGKWPLWLSPRQVLVVPVGVKYFDYA
QKVRDYFHENGFFADVDVGPNTLQKKVRQGQLQQYNYLLVVGEEEESSNTVNIRKRDD
PSKHGKTETVSLEDAVAKMKQLVASKSLVDNVF"
gene complement(<931120..>932925)
/gene="SIF2"
/locus_tag="AWJ20_4920"
/db_xref="GeneID:30037105"
mRNA complement(<931120..>932925)
/gene="SIF2"
/locus_tag="AWJ20_4920"
/product="Sif2p"
/transcript_id="XM_018882025.1"
/db_xref="GeneID:30037105"
CDS complement(931120..932925)
/gene="SIF2"
/locus_tag="AWJ20_4920"
/inference="similar to AA sequence:KEGG_Orthology:K04508"
/note="WD40 repeat-containing subunit of Set3C histone
deacetylase complex; complex represses early/middle
sporulation genes; antagonizes telomeric silencing; binds
specifically to the Sir4p N-terminus; GO_component:
GO:0070210 - Rpd3L-Expanded complex [Evidence IDA] [PMID
19040720]; GO_component: GO:0034967 - Set3 complex
[Evidence IDA] [PMID 11711434]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 9651685]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0016568 - chromatin modification [Evidence IEA];
GO_process: GO:0016575 - histone deacetylation [Evidence
IDA] [PMID 11711434]; GO_process: GO:0031939 - negative
regulation of chromatin silencing at telomere [Evidence
IMP,IPI] [PMID 9651685]; GO_process: GO:0045835 - negative
regulation of meiosis [Evidence IPI] [PMID 11711434];
GO_process: GO:0032874 - positive regulation of
stress-activated MAPK cascade [Evidence IMP] [PMID
18487345]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IMP] [PMID
19379692]"
/codon_start=1
/product="Sif2p"
/protein_id="XP_018736444.1"
/db_xref="GeneID:30037105"
/translation="MGEEEEKRLRELESQNSSNNHKPDEKVSAIEAENGSANAKSITQ
NSTQDHENGETDESQVATNKSHHARDHHKVGLLGNVKSNSLALHTLRPSYSLEGSQTS
QFNPVKSNLVAYGTVEGARILSYKEKSRSPQTVDADKDVEMKDIDSTSAVAVAVTDAS
NSKATSNTDSKIDDSNAISTNKPTDETSKKDNSEANSNSNTNSSLITETIVLTHAPNE
DKEITALTWSPNGTILATGSFDGKIRIWTSEGKLRSLLALHRAPILAIRFNPGGRLLG
SVDCTGTVIVWEVSNGEIREFHGEKDLANSLTNHQPVAQSETSITSSNLQTQQPHSQT
QSPTHQYPSQSPSLSSHGQDASKFTANNVETETESDQPNGGDLTWIDDTTYVTTGQSQ
SILVYKLGEQQPLLRLSAHTQSINSLQFEPSTKLLASASDDYTVRIWHGISPVSIMTL
AGHTAPVIDIKWLSGSALEHSSLPLLISSSIDSTIRIWDIAKGTCISLLKLHDSPLFT
TELSPNNRYIASGSAGGELVVWDIQEVLKYDANSHQTDSIVCRPIGRHKLDQSPSDPV
PTDTTVSSLSWSPASDGLFVGYTTVSEIIPFSLII"
gene <934139..>936700
/gene="EXO84"
/locus_tag="AWJ20_4921"
/db_xref="GeneID:30037106"
mRNA <934139..>936700
/gene="EXO84"
/locus_tag="AWJ20_4921"
/product="Exo84p"
/transcript_id="XM_018882026.1"
/db_xref="GeneID:30037106"
CDS 934139..936700
/gene="EXO84"
/locus_tag="AWJ20_4921"
/note="Exocyst subunit with dual roles in exocytosis and
spliceosome assembly; subunit of the the exocyst complex
which mediates polarized targeting and tethering of
post-Golgi secretory vesicles to active sites of
exocytosis at the plasma membrane (PM) prior to
SNARE-mediated fusion; required for exocyst assembly and
targeting the complex to specific sites on the bud tip PM;
associates the U1 snRNP; role in pre-mRNA splicing and
prespliceosome formation; possible Cdc28 substrate;
GO_component: GO:0005933 - cellular bud [Evidence IEA];
GO_component: GO:0005935 - cellular bud neck [Evidence
IEA]; GO_component: GO:0005935 - cellular bud neck
[Evidence IDA] [PMID 10438536]; GO_component: GO:0005934 -
cellular bud tip [Evidence IDA] [PMID 10438536];
GO_component: GO:0031410 - cytoplasmic vesicle [Evidence
IEA]; GO_component: GO:0000145 - exocyst [Evidence IDA]
[PMID 10438536]; GO_component: GO:0000131 - incipient
cellular bud site [Evidence IDA] [PMID 10438536];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 19053807]; GO_component: GO:0005628 - prospore
membrane [Evidence IDA] [PMID 24390141]; GO_component:
GO:0030133 - transport vesicle [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0006893 - Golgi to plasma membrane
transport [Evidence IMP] [PMID 10438536]; GO_process:
GO:0006893 - Golgi to plasma membrane transport [Evidence
IMP] [PMID 15788396]; GO_process: GO:0001927 - exocyst
assembly [Evidence IMP] [PMID 15788396]; GO_process:
GO:0051601 - exocyst localization [Evidence IMP] [PMID
15788396]; GO_process: GO:0006887 - exocytosis [Evidence
IEA]; GO_process: GO:0006887 - exocytosis [Evidence
IGI,IMP] [PMID 10438536]; GO_process: GO:0006887 -
exocytosis [Evidence IMP] [PMID 15788396]; GO_process:
GO:0015031 - protein transport [Evidence IEA]; GO_process:
GO:0000245 - spliceosomal complex assembly [Evidence
IMP,IPI] [PMID 11425851]; GO_process: GO:0006810 -
transport [Evidence IEA]; GO_process: GO:0090522 - vesicle
tethering involved in exocytosis [Evidence IC] [PMID
10438536]"
/codon_start=1
/product="Exo84p"
/protein_id="XP_018736445.1"
/db_xref="GeneID:30037106"
/translation="MSELPALSLRKHRQTMISDAKRPDLSQFAGIPGQQPASQDSTLQ
LPTVGIKDREKVGKLVKKRLSSRPTPQMYGKDAGSAPAMPVNAHDLILEVSNNDYPLP
PKQRYNNNHAGGIDKASVNRDDFDGDERTGARTGPGSGTTGNFYPVPLGPGSGIPPGD
KTEGTKGLGPGHGPVKTAPDLPSKDYPDREYPSQPGHGVPYGQNDRRHLASTAIPIQN
DRKPFLAPDFDAKTFISDNLSNATDVEISEFAKKLSSLKEDLVQEKKDEMYSNYQKFL
LVGRTISGLNTDLETLRKILNDLNLATSALKDDAARHLSLNNNNSSSTQLNRNMSLSA
NESKMSGLSDSSGTSSGPGAGSSLSPSSAGYDRRASVLAMEEFWQRNLTSLFRHVEGA
QKYLPIIPGRHVIKEQGGWYELNAATWKPLQPAHMFLLNDNLLIATKKRSREHNSASV
QPTRALIANQCWSLLDIDVTEVKSYPQIGQRVLQRAISIKFESMTYVYRAEKSENHDV
FLQEFRRVRDELYQLKNSRRTNGLHSHTRSASGSSFNNIPPPSPSTGGGAGVGPIRRH
NGVARSDSLSLLPSSASSSSKSALVREVDQTLSELDLKIAHRQFSAVVDMILKSKPRF
QQVSDTLSAETSVPIPRPSTPKSSSNLTVGSGAGAGAGTATGPAGSSYTQFTGDILPI
DKLEARITTTADLLLQRLSEEYLSRKELGEFVKMLTQLDAADSAKTTLMNMRHFVIQR
RIKQVPFQGDIPGYIRQVAFITFQLIVSTVEIYRACFPDPQDSSTIAVWAKAEVDSYS
MLFARQLHNVNPSTATYQQCCDITRDESACLKTVNLDFSFMLDYMFDATAAKRKH"
gene complement(<937041..>938000)
/gene="TRI1"
/locus_tag="AWJ20_4922"
/db_xref="GeneID:30037107"
mRNA complement(<937041..>938000)
/gene="TRI1"
/locus_tag="AWJ20_4922"
/product="Tri1p"
/transcript_id="XM_018882027.1"
/db_xref="GeneID:30037107"
CDS complement(937041..938000)
/gene="TRI1"
/locus_tag="AWJ20_4922"
/inference="similar to AA sequence:KEGG_Orthology:K15223"
/note="Non-essential sumoylated hypothetical protein;
similar to components of human SWI/SNF complex including
SMRD3; green fluorescent protein (GFP)-fusion protein
localizes to the cytoplasm, nucleus and nucleolus; TRI1
has a paralog, UAF30, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003677 - DNA binding [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0008150 - biological_process [Evidence
ND]; GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Tri1p"
/protein_id="XP_018736446.1"
/db_xref="GeneID:30037107"
/translation="MNEALTIGQAILGVSDLSVISSKRIRRSLQELLNQDLTAYKKEL
DNVIVERFVLIQEKRNHADTNSEDGQKTGVQNPRNGTDHEKPSNGHSSNGTDSHKNSQ
KNHQSQTTNGTARKTVASGPGSDPLSSPNSSLSDSSSAVTGLNTPAKSVSSKTTTASK
SSKPKPSVKRSVDDDAEMAAKLHAELNRPSLRRETRQPASKRSRKTKKRRADDDSDED
KPKRKANPNNPFMRPVVLSPALSTVLGETEMPRPHIVKALWKYIKEHKLQNPENMKEI
ICDDTLRPVFGSSKCGTPASGGWGSAPDPVAPLAPLESVARGL"
gene complement(<938658..>941835)
/gene="CAB2"
/locus_tag="AWJ20_4923"
/db_xref="GeneID:30037108"
mRNA complement(join(<938658..940304,940996..>941835))
/gene="CAB2"
/locus_tag="AWJ20_4923"
/product="phosphopantothenate--cysteine ligase CAB2"
/transcript_id="XM_018882028.1"
/db_xref="GeneID:30037108"
CDS complement(join(938658..940304,940996..941835))
/gene="CAB2"
/locus_tag="AWJ20_4923"
/inference="similar to AA sequence:KEGG_Orthology:K01922"
/note="Subunit of the CoA-Synthesizing Protein Complex
(CoA-SPC); subunits of this complex are: Cab2p, Cab3p,
Cab4p, Cab5p, Sis2p and Vhs3p; probable
phosphopantothenoylcysteine synthetase (PPCS), which
catalyzes the second step of coenzyme A biosynthesis from
pantothenate; null mutant lethality is complemented by E.
coli coaBC (encoding a bifunctional enzyme with PPCS
activity); GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14690591]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14690591]; GO_function: GO:0016874 -
ligase activity [Evidence IEA]; GO_function: GO:0004632 -
phosphopantothenate--cysteine ligase activity [Evidence
IEA]; GO_function: GO:0004632 -
phosphopantothenate--cysteine ligase activity [Evidence
ISS] [PMID 11923312]; GO_process: GO:0015937 - coenzyme A
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0015937 - coenzyme A biosynthetic process [Evidence
ISS] [PMID 11923312]; GO_process: GO:0015937 - coenzyme A
biosynthetic process [Evidence IGI] [PMID 19266201]"
/codon_start=1
/product="phosphopantothenate--cysteine ligase CAB2"
/protein_id="XP_018736447.1"
/db_xref="GeneID:30037108"
/translation="MIHTSGPKAETAVDREAPEFPPAQSTDEDSYFKHNDEPSYLASL
QVQVAEFIKIHKDRKIALVTSGGTTVPLENNTVRFIDNFSAGTRGSTSAEYFLEAGYA
VIFLHRQFSLLPYSRHYSHSTNCFLDYMVEGNDNHSVQVDKKYEEQMLQVLKKYQYAK
RNKLLLLVPFTTVNQYLFTLRMISQELGQQKAQNRSTTLFYLAAAVSDFFLPRSRVAD
HKIQSQGTGKLVVDLDPVPKFLKRLVDEWTPGAMIVSFKLETDSSLLIGKARGALERY
SHQLGKKGDEVDQAEIERQVKAAVAAKEYFSELLERNASRHVQVRAIRAVGGGARSTR
REFLNQQFEPIFQARNLSSLLSAVDTVYDNLAGLGIYENVTVALDETKEKVNGNGVRG
LDNLDPFAGLFGNDSGRAYDNNERRNSGSKLELDAVVKLAQAKRFYAKTGTDLGNGEG
NGYANMTFKNIFGGAEILSLDATMGTRTRSSYIANFSTPVLSVLPPQWRLETVAYMTS
RTIPWASNEQLVRGMSTKIKSVPSLLKRPGQTPSPQQELGFEAVWRTIACSPGASSSV
RDEAGNSLKASIFHSIAYDTRDSILLATHGNYYKFSHELAGLGGIGNAFYKTSFESQH
NWLLFASPPTTSSSSSEPSTISSQQTTEPVSRTSLHIGTKAGLLWMLARPSSNSDTPP
PSNLMDRFYLGGPNDVRGFYSSGIGPRDQSDSLGGEALLAAGVSLLTPIPKIKRTTPL
RFHTFLNAGNLLALDRIGSSTTTAPTTSSILSSTAAELLNTPSISTGVGLLYCHPAAR
FELNITFPLIARRNEATRKGIQFGVGLSFL"
gene <949197..>950243
/gene="PUB1"
/locus_tag="AWJ20_4924"
/db_xref="GeneID:30037109"
mRNA <949197..>950243
/gene="PUB1"
/locus_tag="AWJ20_4924"
/product="Pub1p"
/transcript_id="XM_018882029.1"
/db_xref="GeneID:30037109"
CDS 949197..950243
/gene="PUB1"
/locus_tag="AWJ20_4924"
/inference="similar to AA sequence:KEGG_Orthology:K13201"
/note="Poly (A)+ RNA-binding protein; abundant
mRNP-component protein that binds mRNA and is required for
stability of many mRNAs; component of glucose deprivation
induced stress granules, involved in P-body-dependent
granule assembly; protein abundance increases in response
to DNA replication stress; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 8413212]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 8413213];
GO_component: GO:0000932 - cytoplasmic mRNA processing
body [Evidence IDA] [PMID 23222640]; GO_component:
GO:0010494 - cytoplasmic stress granule [Evidence IDA]
[PMID 18981231]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 8413212]; GO_function: GO:0003723 -
RNA binding [Evidence IEA]; GO_function: GO:0003729 - mRNA
binding [Evidence IDA] [PMID 15964806]; GO_function:
GO:0003729 - mRNA binding [Evidence IDA] [PMID 23222640];
GO_function: GO:0003729 - mRNA binding [Evidence IDA,IMP]
[PMID 8413212]; GO_function: GO:0003729 - mRNA binding
[Evidence IDA,IMP] [PMID 8413213]; GO_function: GO:0003676
- nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0008266 - poly(U) RNA binding [Evidence
IDA] [PMID 8413213]; GO_process: GO:0000184 -
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [Evidence IDA] [PMID 10892745];
GO_process: GO:0043488 - regulation of mRNA stability
[Evidence IMP] [PMID 15964806]; GO_process: GO:0034063 -
stress granule assembly [Evidence IMP] [PMID 18981231]"
/codon_start=1
/product="Pub1p"
/protein_id="XP_018736448.1"
/db_xref="GeneID:30037109"
/translation="MVNNHEIKINWAFQTQQAIKDLDFFNIFVGDLSSDVNDELLSQT
FNHFPSLVEARVMWDMTTGRSRGYGFVSFKDEADATTALETMDGKLIGSRAIRLNWAQ
QKVGGMAGGPGGGPGGYQNQQGHHHHHHHHHPHSHHQQPYYNNSGGRQGGGSGRRGGP
GGASGGPGGGYGPGGGYGGGPGGGGGGAGGYGGAGGSYGSSGGSYGGSGGYSGPNSGI
SVGPSHSGTSSGAASVSSSSDSGQVPAGAMTYDMVFHATPNWQTTVYVGNLAPYTPPN
DLLSVLQSFGYIVDFKFQPERGYAFVKFDTHERAAMAIVQLSGYQFNGRLLKCGWGKD
RNNSHSNYQPYRQY"
gene <951662..>953401
/gene="UBP1"
/locus_tag="AWJ20_4925"
/db_xref="GeneID:30037110"
mRNA <951662..>953401
/gene="UBP1"
/locus_tag="AWJ20_4925"
/product="Ubp1p"
/transcript_id="XM_018882030.1"
/db_xref="GeneID:30037110"
CDS 951662..953401
/gene="UBP1"
/locus_tag="AWJ20_4925"
/inference="similar to AA sequence:KEGG_Orthology:K11870"
/note="Ubiquitin-specific protease; removes ubiquitin from
ubiquitinated proteins; cleaves at the C terminus of
ubiquitin fusions irrespective of their size; capable of
cleaving polyubiquitin chains; GO_component: GO:0005737 -
cytoplasm [Evidence IC] [PMID 1429680]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 15635103];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 15635103]; GO_function: GO:0008234 -
cysteine-type peptidase activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0008233 - peptidase activity
[Evidence IEA]; GO_function: GO:0004843 -
ubiquitin-specific protease activity [Evidence IDA] [PMID
1429680]; GO_process: GO:0016579 - protein
deubiquitination [Evidence IDA] [PMID 1429680];
GO_process: GO:0016579 - protein deubiquitination
[Evidence IDA] [PMID 2050695]; GO_process: GO:0006508 -
proteolysis [Evidence IEA]; GO_process: GO:0006511 -
ubiquitin-dependent protein catabolic process [Evidence
IEA]"
/codon_start=1
/product="Ubp1p"
/protein_id="XP_018736449.1"
/db_xref="GeneID:30037110"
/translation="MLSDTIKGSMDMASASMKYLYDKAATKVLGPMPFDESELTEAEK
ANGIEEMRMYNVGGIYNEGNTCFMNSVVQSFASLQYLEEFLDDSLGIPRTQRVLPELK
KPVIPESASDGLTENAGETGTSIASTANDNSGLNGSLESADTLNGGSGNYEQGGQSDK
VESRAGTLPETQLKLHQPSTNGYFSLPRNESQRPMTPGTAKVSLLKTLLSLMSQLNEK
KPSSHTYTTTQLIKSISYVLGKDADVLTGYDQEDAQEFFQQILSCLEKEVKKTPTSNT
AWEQRRLRESSVETNASIDNETNGSANSASSNNSSNNNNNEKLLTPFDGVFATRVGCV
KCGEMEGIRRGTLSSVDLSLQNGGDDFELEGLLAEYCAMETISGVECYRCSLNAYKTE
LETRLENQTVGALRNLYATRVEELKSALSDLVIDEAKYAKLKPTKLKELGDKTKQTMF
AMPCADIMMIHINRSVFDLNTGYSRKNYAPVRFPVQLDMSPFIVDTNDPVNRDPSKPM
AHIASGRRYLYNLKATVIHYGSHNFGHYVCFRKCEQGFWWRISDHSVDLTTEHEVLRT
QGVFMLFYERDHE"
gene complement(<954209..>955822)
/gene="GUT1"
/locus_tag="AWJ20_4926"
/db_xref="GeneID:30037111"
mRNA complement(<954209..>955822)
/gene="GUT1"
/locus_tag="AWJ20_4926"
/product="glycerol kinase"
/transcript_id="XM_018882031.1"
/db_xref="GeneID:30037111"
CDS complement(954209..955822)
/gene="GUT1"
/locus_tag="AWJ20_4926"
/inference="similar to AA sequence:KEGG_Orthology:K00864"
/note="Glycerol kinase; converts glycerol to
glycerol-3-phosphate; glucose repression of expression is
mediated by Adr1p and Ino2p-Ino4p; derepression of
expression on non-fermentable carbon sources is mediated
by Opi1p and Rsf1p; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 17054397]; GO_function:
GO:0005524 - ATP binding [Evidence IEA]; GO_function:
GO:0004370 - glycerol kinase activity [Evidence IEA,IEA];
GO_function: GO:0004370 - glycerol kinase activity
[Evidence IMP,ISS] [PMID 8358828]; GO_function: GO:0016301
- kinase activity [Evidence IEA]; GO_function: GO:0000166
- nucleotide binding [Evidence IEA]; GO_function:
GO:0016773 - phosphotransferase activity, alcohol group as
acceptor [Evidence IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0005975 - carbohydrate metabolic process [Evidence
IEA]; GO_process: GO:0019563 - glycerol catabolic process
[Evidence IEA]; GO_process: GO:0006071 - glycerol
metabolic process [Evidence IEA]; GO_process: GO:0006071 -
glycerol metabolic process [Evidence TAS] [PMID 8358828];
GO_process: GO:0006072 - glycerol-3-phosphate metabolic
process [Evidence IEA]; GO_process: GO:0016310 -
phosphorylation [Evidence IEA]"
/codon_start=1
/product="glycerol kinase"
/protein_id="XP_018736450.1"
/db_xref="GeneID:30037111"
/translation="MSPEPTVLEVAGELQMASIDRDAFKNVSDVPVDEEQYVLALDQG
TTSARVIVFSSESVPVASHQVELNQHYPHPGWVEHDPMELIRACRECIDKVGDKLDAK
RIPRKNVKALGITNQRETTVLWDKRNGQPVYNAIVWSDTRTEGTVVRLEQKPNADKVP
KICGLHISTYFAAVKVRWILDNVPKAREVYEEGCLCFGTVDSWILYNFTNGARHITDA
TNASRSMFMNIQTLEYDAELVDFFDVPKLNLPDVVSSSEIYGYFSDDDAAFPSLPISG
CLGDQSSALVGHLGFDKGDAKNTYGTGCFLLYNTGNDPVTSENGLLTTIAFQLKGQKP
VYALEGSIAVAGSIIKWFRNNLGLIETSEQMSDLAGQVKDSAGVYFVTAFSGLFAPYW
RRDARGTIVGLTAYTTRAHIARAAIEATCFQTRAILESMSRDSGVPFHKLLVDGGMSS
SAVAMQIQSDILGMEVVRPGMREPTALGAAIVAGLATGVWNDLDHVRSKLQKRRTITS
FTGSLTEKERDEQYRMWGRAVEKACGWVE"
gene complement(<961417..>962115)
/locus_tag="AWJ20_4927"
/db_xref="GeneID:30037112"
mRNA complement(<961417..>962115)
/locus_tag="AWJ20_4927"
/product="hypothetical protein"
/transcript_id="XM_018882032.1"
/db_xref="GeneID:30037112"
CDS complement(961417..962115)
/locus_tag="AWJ20_4927"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736451.1"
/db_xref="GeneID:30037112"
/translation="MDQYIYNPPPPPPPPAPGSGVGSARKTNSRGNQTNSINSYHNRA
DSRQHPYKKPNIGAATSFGAASGSAMGGASGGGSVKMDGDLSSISPEIREAAKDIENW
IAERRKKWPSAQRIVEKEKEDQQRQEREKKRHEFRLEHEGASSTAITASSTSSSSTKP
LCRYFLKGKCKAGTKCQYSHDKTNPSTSTPKYKRFEAPDRSSLFKKLVQTDLDRENSR
ILDFLVYLGETGVL"
gene <963337..>963648
/gene="FMP37"
/locus_tag="AWJ20_4928"
/db_xref="GeneID:30037113"
mRNA <963337..>963648
/gene="FMP37"
/locus_tag="AWJ20_4928"
/product="Mpc1p"
/transcript_id="XM_018882033.1"
/db_xref="GeneID:30037113"
CDS 963337..963648
/gene="FMP37"
/locus_tag="AWJ20_4928"
/codon_start=1
/product="Mpc1p"
/protein_id="XP_018736452.1"
/db_xref="GeneID:30037113"
/translation="MCTTHFWGPVSNFGIPVAAIMDLKKDPTLISGPMTGSLIVYSLV
FMRYATAVTPWNPLLFGCHVVNEIAQIGQGARFVNYWYLGGKDKATTAPAAPAASSEE
K"
gene complement(<963887..>964933)
/locus_tag="AWJ20_4929"
/db_xref="GeneID:30037114"
mRNA complement(<963887..>964933)
/locus_tag="AWJ20_4929"
/product="hypothetical protein"
/transcript_id="XM_018882034.1"
/db_xref="GeneID:30037114"
CDS complement(963887..964933)
/locus_tag="AWJ20_4929"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736453.1"
/db_xref="GeneID:30037114"
/translation="MKNYLELMAQRAEIFEITFGAEHVQLSSYTTGIYTSESTILRQP
LNTQMIMRLDQIEALAVEENMSLGIRLKEFRVFISLLDSLSPQSLFEAYFFKAGYPML
FSLSDQAVISLPVQQLNPGGDSTGGGGPAKAVPRGIDISFVFLTTGPSTGSDIGTTST
TSHLPSDSDAIVESRNVSIIRRGISRAAAASDTMTTSGSTTTAAESIIGARSMSTVTD
TDSLTSGGRNHNHNRRAYNFDGDDPSGSLTASGSMLVDSAHELNNNNNDDYEIQPSVR
WNDNDDHELMAPDQTMHQQQPQPSTTESSLFVYQENDDEEEALDHELLTFASQLDAIG
PTQNKSQAHGLFDD"
gene complement(<965985..>967706)
/locus_tag="AWJ20_4930"
/db_xref="GeneID:30037116"
mRNA complement(<965985..>967706)
/locus_tag="AWJ20_4930"
/product="hypothetical protein"
/transcript_id="XM_018882036.1"
/db_xref="GeneID:30037116"
CDS complement(965985..967706)
/locus_tag="AWJ20_4930"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736454.1"
/db_xref="GeneID:30037116"
/translation="MSWADEKKGVEAQLPVPVLEPRVESRETQKKKKKTWSLIFSVVA
GVIFVGLFRSIVSSGFWPHFRLRFPHHCHGSDGDESGDLVWKELAKGSNVLHANITLP
LDYNDRSAGNVTVALNKIPATHKDGSLGSILLNPGGPGGSGTNLLYRRGADISEIVEG
RYDIIGFDPRGINWTSPNFYCGVGDKQISGFNAISNELLSTHGDNVSYGEQLAYGKLQ
GLACSSDDSGKYISTALVSTDMLHISRALGDEKLNYWGFSYGSILGNTFAQMYPESVG
RMIVDGVMDAEDYYAGTWFSNLENTDNVTRTFYEACSASTNCPIEGSPDEIEHLFGDL
ITKLKFEPATVFDIESGSVGLVTYSGFLSSFFATLYSPVVWNRFANVTLDLLNGNALP
FYQMYLAPGPDADSLADSAGYATLCGDASNATYPKTVTSPEALKNLVATLRKSSKWWS
DNWLIASGCFGYKQTPVMLWDGPFQKVNASMLLIGNTYDPVTPISAARKMATFYTDSA
VIHQSGVGHCSLAQKSSCTAELVRNWFNNGVLPPPNTVCPTDEVPFETAQEATVTDLF
IPRFFSV"
gene complement(<968037..>968618)
/gene="GEP4"
/locus_tag="AWJ20_4931"
/db_xref="GeneID:30037117"
mRNA complement(<968037..>968618)
/gene="GEP4"
/locus_tag="AWJ20_4931"
/product="Gep4p"
/transcript_id="XM_018882037.1"
/db_xref="GeneID:30037117"
CDS complement(968037..968618)
/gene="GEP4"
/locus_tag="AWJ20_4931"
/inference="similar to AA sequence:KEGG_Orthology:K01094"
/note="Mitochondrial phosphatidylglycerophosphatase (PGP
phosphatase); dephosphorylates
phosphatidylglycerolphosphate to generate
phosphatidylglycerol, an essential step during cardiolipin
biosynthesis; null mutant is sensitive to tunicamycin,
DTT; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005743 - mitochondrial inner membrane
[Evidence IEA,IEA]; GO_component: GO:0005759 -
mitochondrial matrix [Evidence IDA] [PMID 20485265];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0008962 -
phosphatidylglycerophosphatase activity [Evidence IEA];
GO_function: GO:0008962 - phosphatidylglycerophosphatase
activity [Evidence IDA,IGI,IMP] [PMID 20485265];
GO_process: GO:0032049 - cardiolipin biosynthetic process
[Evidence IMP] [PMID 20485265]; GO_process: GO:0006629 -
lipid metabolic process [Evidence IEA]; GO_process:
GO:0006655 - phosphatidylglycerol biosynthetic process
[Evidence IEA]; GO_process: GO:0008654 - phospholipid
biosynthetic process [Evidence IEA]; GO_process:
GO:0046838 - phosphorylated carbohydrate dephosphorylation
[Evidence IMP] [PMID 20485265]"
/codon_start=1
/product="Gep4p"
/protein_id="XP_018736455.1"
/db_xref="GeneID:30037117"
/translation="MNVSGTVNALRLFYNPWQFLPQITVSTFNQIPIPIPSANGKKPI
RVVILDKDNCFAEPHKNVVWPEYEQHFAKLKEAYPDGVLIVSNTAGSGDDVGYKQAEL
LEQNTGIKVLHHTSKKPGCHSEIMDYLRTQNLATDASEVAVVGDRLMTDVLMANLMGS
TSVWIRDGVVPPTSVLSRVEYALYDSLANKDQK"
gene complement(<972542..>973846)
/locus_tag="AWJ20_4932"
/db_xref="GeneID:30037118"
mRNA complement(<972542..>973846)
/locus_tag="AWJ20_4932"
/product="hypothetical protein"
/transcript_id="XM_018882038.1"
/db_xref="GeneID:30037118"
CDS complement(972542..973846)
/locus_tag="AWJ20_4932"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736456.1"
/db_xref="GeneID:30037118"
/translation="MISQHSDIVGKSRAARRVDVDFGFSRGPGGSSSSSGSGAGGNNS
GFGSQLSTIPPGLSVGGNNTGGSARGSGGAIIGVASGASAASGLGAGVGGGSNGSYPP
SRTTTPALDPESFPTLGSSVQSSNSSSSNSRLPASVTAYHANLPPSDNSPEMLKRRLE
ERARQYLNYDTSKMAKFQELTSKYKDGQLDSKMVVFEYNRLFDIKPAQLELLIQDLAV
LYKNLPSKRNALISAFDDWKSKQAFPALALASSASTSPSFSSSSGLSHSLPGSKTNLS
SSWVSANRATPGSIKTSLASSSHFPSLSGATPSAAKPPPVSSSSPGNLSPVSATAKSF
STASLIGSAKVIPRASPSPAAAAKLSDSSFPSLPAAKPKRLPPVRAKTTSTPGPSVWT
SGLSATPVDDSPPLSSSTPPPTSNKKKGKGKQVLFRLGAFEN"
gene complement(<975920..>979045)
/gene="CRZ1"
/locus_tag="AWJ20_4933"
/db_xref="GeneID:30037119"
mRNA complement(<975920..>979045)
/gene="CRZ1"
/locus_tag="AWJ20_4933"
/product="DNA-binding transcription factor CRZ1"
/transcript_id="XM_018882039.1"
/db_xref="GeneID:30037119"
CDS complement(975920..979045)
/gene="CRZ1"
/locus_tag="AWJ20_4933"
/codon_start=1
/product="DNA-binding transcription factor CRZ1"
/protein_id="XP_018736457.1"
/db_xref="GeneID:30037119"
/translation="MTDSFQPELVLHQAADTLRAQAPVSGERHLFSVRKISTEPRMVV
GDETRDGVSRGGLRTGAVVTNETITTVTADSGSVPTAPLFPETQKGSLKSSHEESTAA
TKARGEPPVKAFTTNPVSISTESTEVPVPVTVPVSTSTATSPLASRPISPTPATSKST
PSPLLATAVSGQGSDSSSGSSSGSTTETSKPPAVSSTTATATAESSERTTSINIEAEP
KTSAALSPNLGSGPTEDSTSKPLAPTPTLIQKPLKTTTSTDKSDAITTTTSNSDSNAG
SLAASSGLTSTGTDITDSNPVTSSDSVKSPESPSKSPISNTPASKLSPLDTPVINSET
VPSTSHDSATPSTAGPTAGSLVHPTAGAPAEMSTFMHDTGDSAVPSATATRSGSATPS
TTSTGNSSTTTASINPNINTSEDATIVRSDPDSKSLSPTSTTTTSTTPGKHACPYCPQ
TYTRNHNLKSHLLTHSYERPFTCETCSSKFRRLHDLKRHLKLHTGEKPFICEKCGRRF
ARGDALIRHLKSSGQCAGVRRASFVGDVDTGSSGASVAASAGEENIAASSTGVNPGSD
SRGLGSRPDIFDHRNGTGLTVGSISGLKGQELVSHKGDISHFPGDGHASSTSSSGSSR
GPVLVHQFSSGHGHTSSHGNSPTSSGASQGPQGPHGSHSQSPVPLPISASSSDSSSRP
NKVLLLSSSNYSQAVPAPVLVTIQPNNTGPGSSSLPGMEESRLFIHAKPGLIGPRFEP
PASLISYPSHSSSSNNNSHNSQGLPGSRPPAPPVLNHKPLMVDSRSLPPITGTRSYGY
KQPGDLSQQASASATAGASAGASAGASAPSSSVQHQPIAPAPPQLPASTQTTTISSPV
QSDRIQSVQSSTSNHRPGQLQTAVSGHPPAPAPSVHPTNHSLPRENSRIDPVPLQPQT
LSQSHSHLQPQQQQKPQQVQMSTGHSILPQNEHLSPSQTHVPRPPSQPQLSFTRHNPS
PSAADAPAPSTSISFTHTPPLPGTSHEPTDESSTDSLLRVVKILESRVRMLEERLHVT
EGRLSFMESQR"
gene <982375..>984192
/gene="GID7"
/locus_tag="AWJ20_4934"
/db_xref="GeneID:30037120"
mRNA <982375..>984192
/gene="GID7"
/locus_tag="AWJ20_4934"
/product="glucose-induced degradation complex subunit
GID7"
/transcript_id="XM_018882040.1"
/db_xref="GeneID:30037120"
CDS 982375..984192
/gene="GID7"
/locus_tag="AWJ20_4934"
/note="Subunit of GID Complex that binds directly to
central component Vid30p; GID complex is involved in
proteasome-dependent catabolite inactivation of
fructose-1,6-bisphosphatase; Gid7p contains six WD40
repeats; computational analysis suggests that Gid7p and
Moh1p have similar functions; GO_component: GO:0034657 -
GID complex [Evidence IDA] [PMID 18508925]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0045721 - negative
regulation of gluconeogenesis [Evidence IMP] [PMID
12686616]; GO_process: GO:0043161 - proteasome-mediated
ubiquitin-dependent protein catabolic process [Evidence
IMP] [PMID 12686616]"
/codon_start=1
/product="glucose-induced degradation complex subunit
GID7"
/protein_id="XP_018736458.1"
/db_xref="GeneID:30037120"
/translation="MTAIPMNRTRDSNNSSSTSNNSNGNSASSTSNGNSKLASTTGIE
ANGNGNNQNGNSTIASSASITSTESTAATLSERDSSAKNTSGIGTEVLSPTYFGFDKK
ETTRLLIQSLHDLGYHDIGKSLENASGYTIESPQVVDLRNAVLSGEWDNAEQILLAGS
IEVPNPSDINSMIFLIRKQRYLEILEKDDREEALWFLRNQLSPVNGNGHKEELHFLSK
LAMFSLDDIKADAHWDGVNGKSRQLLLESLQEHLSPSIMIPPHRLATLLHQAKSSQLK
ASDYYLGDESSATSDFSLYKDYVGDRTKFPNVTTHILKDHFDEVWYLAFSNDGKYLAS
SSADKRVFIWDVEDNFKLHRRLEGHHKGVVFFSWSPNDKYIVSCGQDSHAIVWDVQKG
EKVSQASHDEAVDSCAWSHDNRSFITGSPDKKMILWDLDGREIHTWTNIRAQSMAVTP
DGNRLIVICSDMIIHVFDLRTYEMITQISVSNKILTSISVSRDSRYAVISVAPEEIHL
WDLETYRLVRKYVGQLQDEFVIRSSFGGLNENFILSGSEDSHVYIWNRDNANLIEALP
GHTGTVNCVQWRPGHPAMFASAGDDSTIRIWQPASRQDL"
gene complement(<984745..>986340)
/gene="ALO1"
/locus_tag="AWJ20_4935"
/db_xref="GeneID:30037121"
mRNA complement(<984745..>986340)
/gene="ALO1"
/locus_tag="AWJ20_4935"
/product="D-arabinono-1,4-lactone oxidase"
/transcript_id="XM_018882041.1"
/db_xref="GeneID:30037121"
CDS complement(984745..986340)
/gene="ALO1"
/locus_tag="AWJ20_4935"
/inference="similar to AA sequence:KEGG_Orthology:K00107"
/note="D-Arabinono-1,4-lactone oxidase; catalyzes the
final step in biosynthesis of
dehydro-D-arabinono-1,4-lactone, which is protective
against oxidative stress; GO_component: GO:0031307 -
integral component of mitochondrial outer membrane
[Evidence IDA] [PMID 16689936]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0031966 -
mitochondrial membrane [Evidence IEA]; GO_component:
GO:0005741 - mitochondrial outer membrane [Evidence IDA]
[PMID 16407407]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 10094636]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003885 -
D-arabinono-1,4-lactone oxidase activity [Evidence
IEA,IEA]; GO_function: GO:0003885 -
D-arabinono-1,4-lactone oxidase activity [Evidence IDA]
[PMID 10094636]; GO_function: GO:0008762 -
UDP-N-acetylmuramate dehydrogenase activity [Evidence
IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0050660 - flavin adenine
dinucleotide binding [Evidence IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA,IEA];
GO_function: GO:0016614 - oxidoreductase activity, acting
on CH-OH group of donors [Evidence IEA]; GO_function:
GO:0016899 - oxidoreductase activity, acting on the CH-OH
group of donors, oxygen as acceptor [Evidence IEA];
GO_process: GO:0009058 - biosynthetic process [Evidence
IEA]; GO_process: GO:0034599 - cellular response to
oxidative stress [Evidence IMP] [PMID 10094636];
GO_process: GO:0070485 - dehydro-D-arabinono-1,4-lactone
biosynthetic process [Evidence IMP] [PMID 10094636];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]"
/codon_start=1
/product="D-arabinono-1,4-lactone oxidase"
/protein_id="XP_018736459.1"
/db_xref="GeneID:30037121"
/translation="MADRLAPFSFANRTHRTWAGTFRCKPQLYIQPRTAEEIRDAVVE
AKRLNKTLMLTGSGHSPSTLTMSDEWIMNLDIFNKVLSIERHESGKFADVTVEAGIRI
YQLTEILAAEGLAIQNLGSISEQSAAGIISTGTHGSSAYHGLVSEQIVKFTLLCSGKA
DPETIECSPGDNLDLFRAGLLSVGKLGIITHVTLRVVPAYNLKSRQQIVSFDRFVDEL
WPTVWTSSEFIRVWWYPYSDRCVLWRADKCKQEEPLREPINNFYGTTLGRFFYESLLW
LAVKVYPSLTPSIERWVFKHQYGFEESINSEGNVAVQRSDLALNMDCLFSQFVNEWAM
PLTEGPKVLRQLETIIKDAAKNNSFYVHAPFEVRISNTAVSGSTDTINPDNYSVPHLG
TVPGNTVRPLLDGTPKLGAATTPITYDKLTLYLNATVYRPFGFDCPIDKWYRTFEDTV
AAVGGKPHWAKNFLGTFDDKPASTLTDGEMRGLKPTMDSWFGDDLTLFKTLRETHDPQ
GTFLSGRHWAEINGFVSSVTDIV"
gene <986836..>988308
/locus_tag="AWJ20_4936"
/db_xref="GeneID:30037122"
mRNA <986836..>988308
/locus_tag="AWJ20_4936"
/product="hypothetical protein"
/transcript_id="XM_018882042.1"
/db_xref="GeneID:30037122"
CDS 986836..988308
/locus_tag="AWJ20_4936"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736460.1"
/db_xref="GeneID:30037122"
/translation="MNRARKRRRDEPWHQLRRGTTERSGGDMRSVREERKGGSGDGSS
GSSLSRHGSSNDITVRDITIDSTGNSGWQRDAVPADLPDEKDAIEKRVARARVRAAIE
KSSDRENSMNTGSNADHGAVNPRETNSGNSETSPRNGVESLRSIAARSVASHSRDIRR
MLLRNSNVSWELGWKLVWEWIRLMGLDSYPLFTLFLKEFGTKRDFHCHHRQTVPVADI
TGYRAGSKRDVFLDSRLLPSINTKHRIEYIPYGGVSGVVSLVQSISQYNLSMEIGNNI
VLLDLSGLRIPRESLNDMCLSLTSLKSLAGLDVSGCGILESSNILTLWRVAMTSGAWT
NLRLLCLGDSYLNSTTMSVYQTLFDTSLAYIEGPSNYTPDETKWVHGRNLTGISPRFT
VNYSLAFKYRFLSSSANINEPRVRLPKATVLQEFILTRDESSPPWLSKRPSFNCRDCY
IRKPTSSSPPTLPSKPASKSKLTIRKDKLRGPLTAQGFLF"
gene <994711..>996717
/gene="PHO84"
/locus_tag="AWJ20_4937"
/db_xref="GeneID:30037123"
mRNA <994711..>996717
/gene="PHO84"
/locus_tag="AWJ20_4937"
/product="phosphate transporter PHO84"
/transcript_id="XM_018882043.1"
/db_xref="GeneID:30037123"
CDS 994711..996717
/gene="PHO84"
/locus_tag="AWJ20_4937"
/codon_start=1
/product="phosphate transporter PHO84"
/protein_id="XP_018736461.1"
/db_xref="GeneID:30037123"
/translation="MPDRSHHNPIPGEIPVPHGVQEYDTVDGRIINDIDDFQYTDKSE
KEIFAYLLQPADAYTPEGVYWADLPFGKKVSFVTSVDSAEAKKELKTIGRMFKEDPLS
PVAWYFRNCVLPGAGLGLEGYVLFSIGNVSSLFSAAWPECWGSKATVCNKNWIAAVSY
LEIVGIIFGQVIVGFIGDGIGRKWGLIQDASIMFIGLIMLTASWGVTLNGWVICYAWS
LFFYSVGVGGEYPMTATSGMERGTRSVFTSADDRLHRGRKVLQAFLMQGWGQFFNQVI
LILLLLIFHHGSGNPPYSKVAVQWTFRIAFAIPAIGTLWLVYYRAYKMPYPSKDLEAV
KKKSKVTGYDVESLKLVLKYFGPRLLATTGAWFANDVFFYGNKLFQGQFIAVIAPNDK
SVILGWLYNLINIGVSLAGYYLAAFLIDNKLYGRNRMMQVGFLMDFILFVIPAFHYKY
YTAPENVAKFQTMYFLSSFFNQFGPNAVTFLVAAEVYPTQVRATAHGISAACGKLGAL
FASVVYSYISVQTRFYLVPWFGLAGMLVTFVFMPDTTGLDLREQDRRFQFIREGRDHE
YHGIAVHPQHLSLWERFRGVGKNYNPELDYKQKVDELRSDWEERQKLKAAEGDNASND
EELTGDYSPDVHQYFVRTSPLLTANKQGVEDNLKLPEAATNEKL"
gene complement(<998023..>998436)
/gene="CUS2"
/locus_tag="AWJ20_4938"
/db_xref="GeneID:30037124"
mRNA complement(<998023..>998436)
/gene="CUS2"
/locus_tag="AWJ20_4938"
/product="Cus2p"
/transcript_id="XM_018882044.1"
/db_xref="GeneID:30037124"
CDS complement(998023..998436)
/gene="CUS2"
/locus_tag="AWJ20_4938"
/inference="similar to AA sequence:KEGG_Orthology:K13093"
/note="Putative checkpoint factor in transcription; binds
to U2 snRNA and Prp11p; may be involved in U2 snRNA
folding; contains two RNA recognition motifs (RRMs);
GO_component: GO:0005686 - U2 snRNP [Evidence TAS] [PMID
11804584]; GO_component: GO:0005681 - spliceosomal complex
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IMP,ISS] [PMID 9710584]; GO_function: GO:0003676
- nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0008380 - RNA splicing [Evidence IEA];
GO_process: GO:0006397 - mRNA processing [Evidence IEA];
GO_process: GO:0000398 - mRNA splicing, via spliceosome
[Evidence IGI,IPI] [PMID 9710584]"
/codon_start=1
/product="Cus2p"
/protein_id="XP_018736462.1"
/db_xref="GeneID:30037124"
/translation="MDRKLADWDDDEPKAISKRWEKVIVLKNAFTLDELQDASTAFDI
KQDIMEGCEDIGPVTSVTLYDLEPEGIATVKFQEKDDAVTCIEVMNGRYFGGKQLEAF
PYDGKIKYRKSKKDGEDDKEQERLDKFGSWLNKGE"
gene complement(<998592..>999323)
/gene="CUS2"
/locus_tag="AWJ20_4939"
/db_xref="GeneID:30037125"
mRNA complement(<998592..>999323)
/gene="CUS2"
/locus_tag="AWJ20_4939"
/product="Cus2p"
/transcript_id="XM_018882045.1"
/db_xref="GeneID:30037125"
CDS complement(998592..999323)
/gene="CUS2"
/locus_tag="AWJ20_4939"
/inference="similar to AA sequence:KEGG_Orthology:K13093"
/note="Putative checkpoint factor in transcription; binds
to U2 snRNA and Prp11p; may be involved in U2 snRNA
folding; contains two RNA recognition motifs (RRMs);
GO_component: GO:0005686 - U2 snRNP [Evidence TAS] [PMID
11804584]; GO_component: GO:0005681 - spliceosomal complex
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IMP,ISS] [PMID 9710584]; GO_function: GO:0003676
- nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0008380 - RNA splicing [Evidence IEA];
GO_process: GO:0006397 - mRNA processing [Evidence IEA];
GO_process: GO:0000398 - mRNA splicing, via spliceosome
[Evidence IGI,IPI] [PMID 9710584]"
/codon_start=1
/product="Cus2p"
/protein_id="XP_018736463.1"
/db_xref="GeneID:30037125"
/translation="MGHTTDPIAKVNFPPIPKGTPSDSLEWAKDPRIAYSQVTGKWIF
EDVDNGDEYEFNEEVRSWVPILEEEDIKSQQQAYQPPSKRSPDREDESKHEDTSTNGS
KRTKKPKLDQPSHKERKPTGIYLSNLPKDVTYEELDTTFSKYGVIAEDLNTGNKKIKI
YRDDKDQPKGDALIVYFKPESVPLAIEMMDNSELRPGEPGRNPNGKIAVQLAEYKHSE
DNHEKPKLNAKEKLKIQKKLQKLNE"
gene complement(<999602..>1001269)
/gene="TPO3"
/locus_tag="AWJ20_4940"
/db_xref="GeneID:30037127"
mRNA complement(<999602..>1001269)
/gene="TPO3"
/locus_tag="AWJ20_4940"
/product="Tpo3p"
/transcript_id="XM_018882047.1"
/db_xref="GeneID:30037127"
CDS complement(999602..1001269)
/gene="TPO3"
/locus_tag="AWJ20_4940"
/note="Polyamine transporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; specific for spermine; localizes to the
plasma membrane; TPO3 has a paralog, TPO2, that arose from
the whole genome duplication; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IMP] [PMID
11171066]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 12562762]; GO_function: GO:0015297 -
antiporter activity [Evidence IEA]; GO_function:
GO:0000297 - spermine transmembrane transporter activity
[Evidence IMP] [PMID 11171066]; GO_process: GO:0000296 -
spermine transport [Evidence IMP] [PMID 11171066];
GO_process: GO:0055085 - transmembrane transport [Evidence
IEA]; GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Tpo3p"
/protein_id="XP_018736464.1"
/db_xref="GeneID:30037127"
/translation="MSSDNSLTEERQIGGYGDITHHYDLTKKISRENNPMEDNDVDPM
YEPPHNYDILRVKSHPDPTHQELNADDPWKYPVDAETGFRIVTFVPDDKDDPRTWSKT
RKWFITLALGVVCFTVAFSSAAVTGAMVGPNAPGATFNVSDEVVILTVTLFVIGFGVG
PLLFAPLSEEVGRRPIYVVTLALAVIFIIPCAVAQNIGTLLVGRFIDGIFFSAPLTII
GGSLADMWVNSERGVAMAIFSAAPFLGPAAGPLVGGFVGDNAGWRWIYYVMLIFAFCV
YVFSTISIPETHHNTLLAKRAKKLIKLTGDTRYKTAKQLVPKSFAETAKVTLQRPPQL
LCEIIVFLVTIYMAVLYGLLYMFFFAYPVIFMEGKGWSASLTGLTFIPIIVGVLISTA
ISPYVNSDYVRRANAYLDKGEVPPAELRLIPMMVGCWFVPIGLFIYAWTSYPTISWVG
PTLAGLPCGFGFILIYNSATNYIVDSYQHYAASALAAKTFVRSFWGASVPLFTIQMYH
RLGYEWAGSLLAFISLACCIIPYLFFFYGAKIRERSKYAYTPEVTKN"
gene complement(<1003096..>1003914)
/locus_tag="AWJ20_4941"
/db_xref="GeneID:30037128"
mRNA complement(<1003096..>1003914)
/locus_tag="AWJ20_4941"
/product="hydrophobic surface binding protein A"
/transcript_id="XM_018882048.1"
/db_xref="GeneID:30037128"
CDS complement(1003096..1003914)
/locus_tag="AWJ20_4941"
/codon_start=1
/product="hydrophobic surface binding protein A"
/protein_id="XP_018736465.1"
/db_xref="GeneID:30037128"
/translation="MKFTTLVLTSLLSSYALADVGSLVSAIKQLQTDVETTDKDVKGF
DKSQGINGALSIQSTEGNIEKDVDSVTSEVNGLGDLSDSDVSQIADAIKSVSSAVTGL
LSDLTSKASDLISVGTQSIVAGDLSSLSGKTYELESAAYKKVPCANAAEIASDFAQIN
SGFDAAFKAYSAQASGSPPAAPSGCASSAAAGGSSAAAATSAAPASSAAATSAAAPAS
SAAAESSAPASTSAAAPATESASSSAPAVAEGGAAGLKAGALGAAAAGVAALLL"
gene complement(<1010664..>1012022)
/locus_tag="AWJ20_4942"
/db_xref="GeneID:30037129"
mRNA complement(<1010664..>1012022)
/locus_tag="AWJ20_4942"
/product="hypothetical protein"
/transcript_id="XM_018882049.1"
/db_xref="GeneID:30037129"
CDS complement(1010664..1012022)
/locus_tag="AWJ20_4942"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736466.1"
/db_xref="GeneID:30037129"
/translation="MATTGASDFSIIALLTPPGAAPGTGLSGVIAGANTMFAAAGGAE
GLGPVIIDQVIGELQGLFGDYPNSTDIAPSAIFLAIFAILGAAHLYIFLKDYSRGHRF
WISFGLFIYCVFRVIGFACRISWAKDVLRINTGIVSVVFSQVSVLYMNVLCMILGHRI
FTWRHPETGAASWFNAIISVIYLLICGVVAMAIVGQAIPYLYFLSPENWARYQKVVKA
AAILNLLYALAGLALVTLAYTFKPGSIDHRLWRMPKMQAKDFLPPTIQPTWIESTGLF
YFPRRGSQIPILKGDRLEKAIRVIPSREAPASGLTNSHSPDHTSGPSIRTAVIVVVSL
SLILTMNVAFRVAAVFTVKPRGGGTPGSHLNAWVFHNYVFYIWYGAFEVIVNLILLLS
RVDLRFYIPDMARKRRADEPLPNHQYENDEVKLTASNNEVSDVENHPQYDGQYNTHNL
AE"
gene complement(<1016263..>1019097)
/gene="STE11"
/locus_tag="AWJ20_4943"
/db_xref="GeneID:30037130"
mRNA complement(<1016263..>1019097)
/gene="STE11"
/locus_tag="AWJ20_4943"
/product="mitogen-activated protein kinase kinase kinase
STE11"
/transcript_id="XM_018882050.1"
/db_xref="GeneID:30037130"
CDS complement(1016263..1019097)
/gene="STE11"
/locus_tag="AWJ20_4943"
/inference="similar to AA sequence:KEGG_Orthology:K11228"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
STE11"
/protein_id="XP_018736467.1"
/db_xref="GeneID:30037130"
/translation="MCLSFGNFPEPLLAGYIVQVLNGLRFLHEQGTIHRDIKGANILT
DKEGKIKLADFGVATQSVVGTLSRDKSVVGTPYWMAPEVIEVNGATAASDIWSLGCTI
VELLTGKPPNYHLDPMSAMYAIVSKEIPLPEGISSELKDFLLECFQKDPNLRVSAKAL
QKHPWLKKHSPSMASSNTKYEEAIKTVKIHNSPKRATSTTLGDDIFSPVPRAEGGKRQ
SPVRNSLFNVSPAKRRTSLVQKGDPPLAKNLAKQFSENWHDDDDEFADLEGDLNADRL
QAVLSSKFASRQPPKPDFKLESEPMQRQTSQTSQVSLESQLSDISLTDESRVSQSNGK
SRSSDIHGYAETNENDYTNDFEDVGTFDTMALSPFIKESPDIRGQSTKESPPSVQEPP
PSAAVSMESTTTADTDNPFLKIESEDVVEADPELRMAHTRLEQVISTMENASNSADLK
NTTEELLDILRNFPETRTTFFKSSGLSLAYTVLRDSTSKTVVVNIVHLLLVIAKDEAN
ANALDDFWLLGGMQVVIRLLSRRKIFQVRSGAATLIEQCCNDSLLSLQSFMACGGLKY
LYELLEEDCQKERVLVRVALTMLKEILQTITSSVLRKSIYKNLCEDGVMNSLVSIPGP
NNASKDLVDLTLNLLLSMSQDSIVRANIGLNYGRKLLSKYDKVQEQKDKLSILKFVKN
ISQEQSHLEPNQEVSIISLLVTILQQATDKASTAIKKDVLNQAVPALFNICRLNGSRQ
EKAAEFGLVPILTNILQDTSIQSRDNVRQLALQMLCDMTHAGKLSREELNRNNCLELL
LTYVPDPYWQRDIYDALGAWLRGDINIQHKLATPPAVDTLLDGLKTNINGISYGDLMN
SFGSMIRLSPLLCKSMPLSKFYSIIRGGLLSTDPLVIVNVLRVLNIVVETTGATVLDA
KLRDQLKKHAKTNNSKLARQLASDLLGPGV"
gene <1023031..>1025604
/gene="RAD16"
/locus_tag="AWJ20_4944"
/db_xref="GeneID:30037131"
mRNA <1023031..>1025604
/gene="RAD16"
/locus_tag="AWJ20_4944"
/product="DNA repair protein RAD16"
/transcript_id="XM_018882051.1"
/db_xref="GeneID:30037131"
CDS 1023031..1025604
/gene="RAD16"
/locus_tag="AWJ20_4944"
/inference="similar to AA sequence:KEGG_Orthology:K15083"
/note="Nucleotide excision repair (NER) protein; binds
damaged DNA during NER; binds DNA in an ATP-dependent
manner (with Rad7p) during NER; required for NER of
non-transcribed chromatin; subunit of Nucleotide Excision
Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS)
ligase complex; GO_component: GO:0031463 - Cul3-RING
ubiquitin ligase complex [Evidence IDA] [PMID 16675952];
GO_component: GO:0000113 - nucleotide-excision repair
factor 4 complex [Evidence IDA] [PMID 9497356];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0005524 - ATP binding [Evidence IEA,IEA];
GO_function: GO:0003677 - DNA binding [Evidence IEA,IEA];
GO_function: GO:0008094 - DNA-dependent ATPase activity
[Evidence IDA] [PMID 9497356]; GO_function: GO:0003684 -
damaged DNA binding [Evidence IDA] [PMID 9497356];
GO_function: GO:0004386 - helicase activity [Evidence
IEA,IEA]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA,IEA]; GO_function: GO:0003676 -
nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0004842 - ubiquitin-protein transferase
activity [Evidence IDA] [PMID 16675952]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0006281 - DNA repair [Evidence IEA]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEA]; GO_process: GO:0000715 -
nucleotide-excision repair, DNA damage recognition
[Evidence IDA,IMP] [PMID 15177043]; GO_process: GO:0042787
- protein ubiquitination involved in ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 16675952]"
/codon_start=1
/product="DNA repair protein RAD16"
/protein_id="XP_018736468.1"
/db_xref="GeneID:30037131"
/translation="MSSGSALSTPPSSIEDDEDWLSMPMSSRQAADLLGVVDSNNGSG
TKRKRPAAKKKSPRQVARDALNSAVNGEEFRLTPEPTAKKTPRKPRMKVASSSEDADF
EEDNSEVTRPRSARSRRKPRIVEASDNDNDDEDDDDEEYKEKSDNSADSEEEVVKEEE
IDELQGEDDNVETAAVVPAPRRRQRRGGKREPRAPKLPRHVVMYNRLNEGLHEFHPEL
KTIFEDMKNRPEIKPEQIPQPEGMACELLPFQREGLNWLIKQEQGMYKGGILADEMGM
GKTIQTVALLMTDPSDAPPTLVVAPTVALMQWKHEIDLHTQGKLSVLLYHGQNRSSDE
SVIQQHRIVLTTYAVLESVFRRQVLGFTRKGSEKKVRQKSPLHQIHWNRIVLDEAHNI
KDRQSNTARAVFNMKANYRLCLSGTPLQNRIGEIYSLMRFLGIEPFSKYYCSKCDCES
HTWNFGSSYHCEICNHVVMHHVNFFNHSLLKHIQRYGFKDEGGVAMGRLRSVLSQIML
RRTKMERADDLGLPPRVVEIRRDYFNEEEKDLYSSVYSESNRKFNTYVAANVVLNNYA
NIFTLITRMRQISDHPDLVLRRTIGNSEDADAINRLVCKICDDEAEEAIQSKCHHTFC
RLCIKEYVEGWTGDQSELECPVCHLQLTIDLTAPAIQADESLVKKGSIVNRINMTGGW
RSSTKIEALVEELYKLRSDRQTIKSIVFSQFTSMLDLVEWRLKRAGFQTVKLQGSMSP
TQRDNIIRHFMETPSVEVFLVSLKAGGVALNLCEASQVFILDPWWNPSVEWQSGDRVH
RIGQHRPVKITRLVIEDSIESRIIELQEKKAQMIQATIESDDGAMNKLTPADMQFLFQ
N"
gene <1025830..>1025916
/locus_tag="AWJ20_4945"
/db_xref="GeneID:30037132"
tRNA <1025830..>1025916
/locus_tag="AWJ20_4945"
/product="tRNA-Thr"
/db_xref="GeneID:30037132"
gene complement(<1026316..>1027365)
/gene="SOR1"
/locus_tag="AWJ20_4946"
/db_xref="GeneID:30037133"
mRNA complement(<1026316..>1027365)
/gene="SOR1"
/locus_tag="AWJ20_4946"
/product="L-iditol 2-dehydrogenase SOR1"
/transcript_id="XM_018882052.1"
/db_xref="GeneID:30037133"
CDS complement(1026316..1027365)
/gene="SOR1"
/locus_tag="AWJ20_4946"
/inference="similar to AA sequence:KEGG_Orthology:K05351"
/note="Sorbitol dehydrogenase; expression is induced in
the presence of sorbitol or xylose; GO_component:
GO:0005575 - cellular_component [Evidence ND];
GO_function: GO:0003939 - L-iditol 2-dehydrogenase
activity [Evidence IEA]; GO_function: GO:0003939 -
L-iditol 2-dehydrogenase activity [Evidence IDA,ISS] [PMID
8125328]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_function: GO:0008270 -
zinc ion binding [Evidence IEA]; GO_process: GO:0019318 -
hexose metabolic process [Evidence IEP] [PMID 8125328];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]"
/codon_start=1
/product="L-iditol 2-dehydrogenase SOR1"
/protein_id="XP_018736469.1"
/db_xref="GeneID:30037133"
/translation="MSNPSFVLQKVNEVSFEDRPVPEIKDPHYVKIAVKKTGICGSDV
HYYTHGAIGDFVVKAPMVLGHESAGVIVEVGSEVKNLKVGDRVAMEPGVPSRYSDEYK
SGRYNLCPCMAFAATPPYDGTLAKYYLLPEDYCVKLPDHVSLDEGALVEPLAVGVHSS
KLADVRPGSKVAVFGAGPVGLLITAVASAFGASSVTVIDIVQNRLDLAKELGATHTVL
ASIKDTSDETAKKVVAATGAQPDIVLDASGAESSINAAISAIKPGGTYVQVGMGKPNV
NFPIANVIGKELTIKGSFRYGYGDYPLAVELISSGKVNVKKLISHTVKFEEAKEAFEL
VMSGKAVKVIIDGPQ"
gene <1030449..>1032098
/gene="LYP1"
/locus_tag="AWJ20_4947"
/db_xref="GeneID:30037134"
mRNA <1030449..>1032098
/gene="LYP1"
/locus_tag="AWJ20_4947"
/product="lysine permease"
/transcript_id="XM_018882053.1"
/db_xref="GeneID:30037134"
CDS 1030449..1032098
/gene="LYP1"
/locus_tag="AWJ20_4947"
/inference="similar to AA sequence:KEGG_Orthology:K03293"
/codon_start=1
/product="lysine permease"
/protein_id="XP_018736470.1"
/db_xref="GeneID:30037134"
/translation="MASTSVNPQPSFGSEIGTTDSKNKNGLSNLSERSFGEPEMGYIV
KSEDRLSRSLSARQVQMIAIAGVIGTGLFLGTGKSLAEAGPASLLIAYFIIGIVVYLT
MNALGEMATYVPVAGSFCTYATRYVDEAFGFALLWNYWFNDAVSTASDLTALQLVFAY
WTDFPSWIIALIFWVFLICANLIHVKVYGELEYWLALLKVVTIVIFMIISIVVNAGAN
TQHEYLGFHYWYIGDAPFVNNFKGFASVFVTASFAYGGTESIAITAGETKNPSRVMPR
VVKTVFWRILFFYVLTVLFIGINVPYNYPNLSTGGTALSPFTITFQLVGSKAAGSFMN
AVIVTSVISAGNHALFAGTRLLYTLGVEGFAPKIFTVLTPHKVPYVALLATASISGLC
FGASFIGTGTLWAWLQNLVGVSNQLAWLSIGVTAIRFRQALNKQGKTHLLKFKNWTYP
WGPWIVVVGVTVIILVQGWSAFAPWSVSSFFSYYIELFIMPVMYIFWKIVKRTKFVKL
EDMDLDTDRYVETEEDLAEMEYEDSLTGWRRVVNYLKGFVV"
gene complement(<1032380..>1035931)
/gene="CHS3"
/locus_tag="AWJ20_4948"
/db_xref="GeneID:30037135"
mRNA complement(<1032380..>1035931)
/gene="CHS3"
/locus_tag="AWJ20_4948"
/product="chitin synthase CHS3"
/transcript_id="XM_018882054.1"
/db_xref="GeneID:30037135"
CDS complement(1032380..1035931)
/gene="CHS3"
/locus_tag="AWJ20_4948"
/inference="similar to AA sequence:KEGG_Orthology:K00698"
/note="Chitin synthase III; catalyzes the transfer of
N-acetylglucosamine (GlcNAc) to chitin; required for
synthesis of the majority of cell wall chitin, the chitin
ring during bud emergence, and spore wall chitosan;
contains overlapping di-leucine and di-acidic signals that
mediate, respectively, intracellular trafficking by AP-1
and trafficking to plasma membrane by exomer complex;
requires AP-3 complex for its intracellular retention;
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 15470103]; GO_component: GO:0005935 - cellular
bud neck [Evidence IDA] [PMID 8909536]; GO_component:
GO:0045009 - chitosome [Evidence IDA] [PMID 8970154];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
9008706]; GO_component: GO:0000131 - incipient cellular
bud site [Evidence IDA] [PMID 15470103]; GO_component:
GO:0000131 - incipient cellular bud site [Evidence IDA]
[PMID 9008706]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0043332 - mating
projection tip [Evidence IDA] [PMID 19053807];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005628 - prospore membrane [Evidence
IDA] [PMID 15755916]; GO_function: GO:0004100 - chitin
synthase activity [Evidence IEA]; GO_function: GO:0004100
- chitin synthase activity [Evidence IMP] [PMID 9760183];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_function: GO:0016757 - transferase activity,
transferring glycosyl groups [Evidence IEA]; GO_function:
GO:0016758 - transferase activity, transferring hexosyl
groups [Evidence IEA]; GO_process: GO:0030476 - ascospore
wall assembly [Evidence IMP] [PMID 1293886]; GO_process:
GO:0034221 - fungal-type cell wall chitin biosynthetic
process [Evidence TAS] [PMID 7565410]"
/codon_start=1
/product="chitin synthase CHS3"
/protein_id="XP_018736471.1"
/db_xref="GeneID:30037135"
/translation="MTEKKDVASSEHKAEVEGNSSLKSGSKSKTSGPGHCKAVLSSGA
TAIAIAKARATAKARARARSKLRTNSRSVSTTNFPTDYYYHLWPVSSVASSVSHNTVL
TGDLRPSGEADRTAETRSEDISDGISEGISMGISEGKSKGTSESLAEGSLDTIDGVGE
RKEENQISKIQAMPVSADAVTGDKPASTGSSTNKSAKDPTEITKLSFSAPEMTANSKS
TAGPRHAPGYPSYFVERFETASAISQPPGPKMSINSQPLNKRLIKSKRAELEFNVGPC
SESSAHIIWTGYCNLISCFVRPIMLRWCGIKSHQQQVAWREKIGLFSVIVLITCTVGV
FTFGFNEMVCGHNSNVPRIHFSSIPPDTVVIHGRLFNMTTFNHNPKFLRPAPWSSNSF
TLTGVSSIPIDASLLFQNVNGHCRPHIKPSRSSQVAHDSDLRVPWYLPCKLLTINDNR
IVDFYVPTISSSAYKQLSFIKKYSPEACHTSKYSRERLYEMNSVSDIYYTWKDIDSSP
RNIAVLFNDVLDLDRLKLLPPEDWELSKALSQFVSLDASQGLPVSDISKYFVADPSLF
KLAKCLTEIAKVGVIDVESVGCISSQIVLYVALMFIFSIVLIKFTFALYFEWFVSWRM
GIKGVSSTSWVFWFIWSLFRAKEKIGTGENSGKSDISLSNSERQYSLKSSESKSSSSL
SNSSVSQTPMETRSESTAFSQMHMFPSTCESLDDQMYVVCLVTVYSESEDGLRLSLDS
LASTDYPDCRKLLLVVCDGLVTGANNDRMTSEIALSLMEDVAVPFHEVPDLSYISVGY
GKRRINMAQVHCGYYAHVPSDSSKPKRRVPMICVVKCGLEEERQTSSKPGNRGKRDSQ
LILMGFFQKLCMGDRKTDLENEIHYCIEHVYGINPQLFEAILMVDADTKVYEDSLGHM
VATLVADSTVIGLCGETKIANKWESWITMIQVFEYFISHHLTKSFESVFGGVTCLPGC
FSMYRIKAPPKSGHVLLLLVSPAVVSRYSDNIVNTVHRKNLLLLGEDRYLTTLMLKAF
PVHKLIFVPQAKCKTIVPSKFKGLLDQRRRWINSTIHNLLELLLVQELCGVFCFSMQF
VVLIDLIGTVTLPAALVFTVYLLIACIYRHPPPVVPLMLLALILGIPGVLILVTAHQW
IYVLWMLIYLISLPVWNFILPLNACKLTAWVLRHNRD"
gene <1037460..>1038506
/gene="URH1"
/locus_tag="AWJ20_4949"
/db_xref="GeneID:30037136"
mRNA <1037460..>1038506
/gene="URH1"
/locus_tag="AWJ20_4949"
/product="trifunctional uridine nucleosidase/nicotinamide
riboside hydrolase/nicotinic acid riboside hydrolase"
/transcript_id="XM_018882055.1"
/db_xref="GeneID:30037136"
CDS 1037460..1038506
/gene="URH1"
/locus_tag="AWJ20_4949"
/inference="similar to AA sequence:KEGG_Orthology:K01240"
/note="Uridine nucleosidase (uridine-cytidine
N-ribohydrolase); cleaves N-glycosidic bonds in
nucleosides; involved in the pyrimidine salvage and
nicotinamide riboside salvage pathways; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
14562095]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0016798 - hydrolase
activity, acting on glycosyl bonds [Evidence IEA];
GO_function: GO:0016799 - hydrolase activity, hydrolyzing
N-glycosyl compounds [Evidence IEA]; GO_function:
GO:0070635 - nicotinamide riboside hydrolase activity
[Evidence IDA] [PMID 19001417]; GO_function: GO:0070636 -
nicotinic acid riboside hydrolase activity [Evidence IDA]
[PMID 19001417]; GO_function: GO:0050263 -
ribosylpyrimidine nucleosidase activity [Evidence IDA]
[PMID 11872485]; GO_function: GO:0050263 -
ribosylpyrimidine nucleosidase activity [Evidence IDA]
[PMID 12111094]; GO_function: GO:0045437 - uridine
nucleosidase activity [Evidence IEA]; GO_function:
GO:0045437 - uridine nucleosidase activity [Evidence IDA]
[PMID 19001417]; GO_process: GO:0034356 - NAD biosynthesis
via nicotinamide riboside salvage pathway [Evidence IGI]
[PMID 19001417]; GO_process: GO:0006216 - cytidine
catabolic process [Evidence IMP] [PMID 23670538];
GO_process: GO:0008152 - metabolic process [Evidence IEA];
GO_process: GO:0019358 - nicotinate nucleotide salvage
[Evidence IGI] [PMID 17482543]; GO_process: GO:0046135 -
pyrimidine nucleoside catabolic process [Evidence IDA]
[PMID 11872485]; GO_process: GO:0046135 - pyrimidine
nucleoside catabolic process [Evidence IDA] [PMID
12111094]; GO_process: GO:0008655 - pyrimidine-containing
compound salvage [Evidence IMP] [PMID 11872485];
GO_process: GO:0006218 - uridine catabolic process
[Evidence IMP] [PMID 23670538]"
/codon_start=1
/product="trifunctional uridine nucleosidase/nicotinamide
riboside hydrolase/nicotinic acid riboside hydrolase"
/protein_id="XP_018736472.1"
/db_xref="GeneID:30037136"
/translation="MSLILPKIPLWIDCDPGHDDAIGLLLGSGLDQYHLVGVSTVHGN
ASLGNTTSNAISILTAFKRLDVNVYPGAEKPLVRKLNVADSIHGRSGLDGTAFLPMPS
FDAQPDHTAVAAMAKAIETYPGTLAIAALGPLTNIALLAMNYPDLLPSLRLLSIMGGG
LGLGNWTKFAEFNIWCDAHAAQIVFSDTTLIPKTLLVPLNLTHQAIATDEILEQIQKS
RQLDRRSVLRQMLYELMIYFAGTYKKKFGFKNGPPVHDAVAVAAILPFYSDNSTERSE
HSNDPPAADLDLRFARYQVHVVEEGPQEGMLVFEPDSDNGIMIAQEMNFTEFWRLVLD
AVDNVESSTSLLVS"
gene complement(<1038815..>1043804)
/gene="GAL11"
/locus_tag="AWJ20_4950"
/db_xref="GeneID:30037138"
mRNA complement(join(<1038815..1043134,1043463..1043527,
1043699..>1043804))
/gene="GAL11"
/locus_tag="AWJ20_4950"
/product="Gal11p"
/transcript_id="XM_018882057.1"
/db_xref="GeneID:30037138"
CDS complement(join(1038815..1043134,1043463..1043527,
1043699..1043804))
/gene="GAL11"
/locus_tag="AWJ20_4950"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; affects transcription by acting
as target of activators and repressors; forms part of the
tail domain of mediator; GO_component: GO:0070847 - core
mediator complex [Evidence IDA] [PMID 9891034];
GO_component: GO:0016592 - mediator complex [Evidence
IEA]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_function: GO:0001102 - RNA polymerase II
activating transcription factor binding [Evidence IDA]
[PMID 20308326]; GO_function: GO:0001128 - RNA polymerase
II transcription coactivator activity involved in
preinitiation complex assembly [Evidence IDA] [PMID
8378310]; GO_function: GO:0001104 - RNA polymerase II
transcription cofactor activity [Evidence IEA];
GO_function: GO:0001135 - RNA polymerase II transcription
factor recruiting transcription factor activity [Evidence
IMP] [PMID 15254252]; GO_function: GO:0001088 -
TFIIE-class binding transcription factor activity
[Evidence IDA] [PMID 8790357]; GO_function: GO:0001095 -
TFIIE-class transcription factor binding [Evidence IDA]
[PMID 8790357]; GO_function: GO:0001097 - TFIIH-class
transcription factor binding [Evidence IDA] [PMID
10973956]; GO_process: GO:0051123 - RNA polymerase II
transcriptional preinitiation complex assembly [Evidence
IDA] [PMID 12482986]; GO_process: GO:0000122 - negative
regulation of transcription from RNA polymerase II
promoter [Evidence IMP] [PMID 11470794]; GO_process:
GO:0045944 - positive regulation of transcription from RNA
polymerase II promoter [Evidence IDA] [PMID 8187178];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 9891034]; GO_process: GO:0006357 - regulation
of transcription from RNA polymerase II promoter [Evidence
IEA]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Gal11p"
/protein_id="XP_018736473.1"
/db_xref="GeneID:30037138"
/translation="MDFNRTGMTFNGMAGAGNNQGQAQAPGQSSNTTQPVGRWGSPVG
FFGVVVKVLTECRQQQQQQQPNQSQMDPSGLLGMLPNQGAASSQFMSGAGGAGANAGA
GANDTNNFNIQMMRNAAATAGGGAGAGATGMEMTPQMQAAFKQFIQQQQQQQQQQQQQ
QQNQGQNQGQNPTAQQLQQQFQQQQQQKNGQFQQHKLQGQVQNALNSGANVNSAAAQR
TQQQQQQFFRQQQQYNQQQQQQQQQQQQQGQGQQQQQPQQQQQLQNMYQTLRGANGGG
PGQVQGQVPQNQNQGQLPTTTGPGANPLAMTREQQFQFLAMQRQQQQQQQQAQAQAQA
QAQAQQNQNLNRSPQVNIGNGQTVNPMTQQNIAGAASAGAGIPGAGLGVNGGGGAATG
VAPNTGAPPNAVNAAAILGPNAMKTAEIPQALLNRIPGLPPNVNTWLQITELLRNGQL
PKSIMPEIRYVHGMHQKWLIHVKNKQLQAQAAAANGGAGAGPAGQRQIPGATTTGVPS
GTGLGATAANGNPAAAGAGGQMGLGANQQLSTADAMMSQLGQLNRNYPGQPSSQTLGM
QNPANAAAVAANQAQLRKTELQQQQLLQTQPQQNQGQQQQQQQAQQQQPSLQQQQYFL
AAQAQARAARAKNSVASNVTNAAKPAMINAQQSPNQMFANLPKAAGAATSSGPGGSVG
SVAGSGATGAGNQFQQWDPNNANFNRPQVSPQMTQMSPQQHQQQLFQNQLDFSGGAAG
VGNNAGGATGVAGTVVPNAPAPVLGPGVAPGLAVAGGATGAPQQRAQMPQQRDADRMK
LRQIFEEAANIPIRLQNFTDTLSAEEKRQVAEQIKLMIHMYQKIDQLVPLFYFITKNE
NATRRLIQLKFMYKEQMEALQKGIYMTSPQILEKTKLEINRYFFFVRDNLQNPQRQQG
LAQRQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQLQKGLIPGQIGTQLPGQVQAPGAG
AGGAGAGGLMNQIPGQANASAAANSMDPQQMFVAQNFGGSMNANLQQQLPQQSVDVNQ
FAQGNLMNQQNNLGPAGLQQQQHLQPQLQQQQQQQHSFVNQPVNQSPQMTKKELAAAA
RKKQASTATAATTGAPVTTTMGPTGPGAAVAGANAGNYQAPGAVHTPSGVPAYVKPGL
TQDQLKLPGPGRKRKLPPGSSQTTALSPEEVKSGNTPKLSPASLPRGRSSSTGEPSLK
KEKLDPNSLTPQQKNALKAMQKKKELADSDPVAYFLTSLGEVLDIPEEDVVNGISKSQ
NGGTTPSGTGVSATGPNNGPGSNEMKKLPTYLGRTSTSGPSGGAGGGVTPNALLRTPL
PFSAAKTPGAVAGGSGLTGGSTIKPGSTNDHLTGSSESGTLKTSSSPNTGLAPAWTGN
VQAVAIRTAFQSIEAVKIGDMPFLASPKEDNNNNTTATTSSSDSSHTSGDSVSSIDAT
AVDEKEPVVLPIDGLDDGKQLKFDGWDDLSGVNIWDYDDALLSLGKDDATLRKEFWTV
ST"
gene complement(<1048115..>1050901)
/gene="YVC1"
/locus_tag="AWJ20_4951"
/db_xref="GeneID:30037139"
mRNA complement(<1048115..>1050901)
/gene="YVC1"
/locus_tag="AWJ20_4951"
/product="Yvc1p"
/transcript_id="XM_018882058.1"
/db_xref="GeneID:30037139"
CDS complement(1048115..1050901)
/gene="YVC1"
/locus_tag="AWJ20_4951"
/note="Vacuolar cation channel; mediates release of Ca(2+)
from the vacuole in response to hyperosmotic shock;
GO_component: GO:0000324 - fungal-type vacuole [Evidence
IDA] [PMID 11427713]; GO_component: GO:0000324 -
fungal-type vacuole [Evidence IDA] [PMID 11781332];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IDA] [PMID 20035756]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005774 - vacuolar membrane [Evidence
IEA]; GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0005227 - calcium activated cation channel
activity [Evidence IDA] [PMID 1700419]; GO_function:
GO:0005262 - calcium channel activity [Evidence IEA];
GO_function: GO:0005262 - calcium channel activity
[Evidence IDA] [PMID 11781332]; GO_function: GO:0005216 -
ion channel activity [Evidence IEA]; GO_function:
GO:0005267 - potassium channel activity [Evidence IDA]
[PMID 1700419]; GO_function: GO:0005272 - sodium channel
activity [Evidence IDA] [PMID 1700419]; GO_function:
GO:0005244 - voltage-gated ion channel activity [Evidence
IDA] [PMID 1700419]; GO_process: GO:0070588 - calcium ion
transmembrane transport [Evidence IEA]; GO_process:
GO:0006816 - calcium ion transport [Evidence IEA];
GO_process: GO:0030003 - cellular cation homeostasis
[Evidence IDA,IMP] [PMID 11427713]; GO_process: GO:0030003
- cellular cation homeostasis [Evidence IDA,IMP] [PMID
11781332]; GO_process: GO:0006811 - ion transport
[Evidence IEA,IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Yvc1p"
/protein_id="XP_018736474.1"
/db_xref="GeneID:30037139"
/translation="MDTEETTLLLPRFQPSALDSIPIYNIVLDIYDLISARVDTALKY
DQLRSPQVHSFLIKPLVADLRDGLCAGTLYGLMANSIQFTKESSSYAAMAGVMATRAM
ICEIVAIKLLKDFDEDDLMNALTYDFYPLARGMPENSSTSQSTQSQLIPRWQRLSTLE
LAIKAEAKRFLAHPVVIQVLEEIWNGSIMFQTSMHKLHRVKFANEEEVLASQFGRRGA
GIRYNYSDASILKLSRLRVPRYRHLLNLASFCVLLGLYLMVLSSHSMKISTQEIVFAL
WSLGFILDEIVGFTDVGFTLYILSLWNLFDLMILLQLIGYACCRINAIRLNATGQIDD
AKKMLTLSYDILATVAIFLFPRLFSIFDNNESFSRMVIAVRKMSIDLAISWLVIVMLS
SGFWIAFTLAFARNVFSSNQVAFDLMRILFGFTPVVWENWKYYSPIGRTILLFYLFIT
HFVVMTLLFAVLSNSFSEIVANSREEHQYLCAVNTISMIKSESSTLFSYSPPLNLIEW
LVRPFFYILPLRQFLILNRTLVKITHFPVLYTIFIYERLYLRLVQIREKVKKDSQNQY
RKKQEERLKALVTQLSSADDEIRSGSATPRKTRKKGKPVSTPVPVPGAVSHTEAVKKH
QLMSKKISKYDLLDEVFKRPYKGTIKVRPKKPKIDLDVDINDSISRPQRNRDMSRLFH
SRAGSSWTSTRPPGTEIYSDPEDTTARPYYESDAIDDEEWPGSYIPNTFERHLSGNSS
IVRRLNDRTSPYLLPTVPSSTVLNKFHNEDNSAQNTRRRLPITSRGRLFSSTSSLFRQ
DSTMLGISPTRSTSSILRRANIHGAVRNRKWGPDHVSDHGKVKRSTNHDDEDNDDDDD
YIVAKSDADVEDEPDQDDEDDRDDGEPTSLAAFNANPVDLWQMQEQIMNRIDSLEGLF
KNIDKKLSQIASQN"
gene <1052006..>1052797
/gene="SPS19"
/locus_tag="AWJ20_4952"
/db_xref="GeneID:30037140"
mRNA <1052006..>1052797
/gene="SPS19"
/locus_tag="AWJ20_4952"
/product="Sps19p"
/transcript_id="XM_018882059.1"
/db_xref="GeneID:30037140"
CDS 1052006..1052797
/gene="SPS19"
/locus_tag="AWJ20_4952"
/inference="similar to AA sequence:KEGG_Orthology:K18337"
/note="Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary
enzyme of fatty acid beta-oxidation; homodimeric enzyme
required for growth and sporulation on petroselineate
medium; expression induced during late sporulation and in
the presence of oleate; GO_component: GO:0005782 -
peroxisomal matrix [Evidence IDA] [PMID 9268358];
GO_component: GO:0005777 - peroxisome [Evidence IEA,IEA];
GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
(NADPH) activity [Evidence IEA]; GO_function: GO:0008670 -
2,4-dienoyl-CoA reductase (NADPH) activity [Evidence
IDA,ISS] [PMID 9268358]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_process:
GO:0030437 - ascospore formation [Evidence IEP,IMP] [PMID
7969036]; GO_process: GO:0009062 - fatty acid catabolic
process [Evidence IDA,ISS] [PMID 9268358]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence IEA];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="Sps19p"
/protein_id="XP_018736475.1"
/db_xref="GeneID:30037140"
/translation="MSGLLANKVAAITGGSSGIGRAIALEFAKNGAKVVINYYPNEQE
TSRAISLGNEIKQLFPNTTEPVYSSVPGDIGDPATGKALVAKAVDEFGKLDVFVSNAG
ICPFHEFTTMPEEVYYRTVKTNLDGAFFSTQAAANQMKLQGTGGSIIGISSISALVGG
EFQTHYTTTKAGVLSLMQSTAVALGKYNIRCNALLPGTIETDINKDDLKDQSKRDYFN
KRIPLGRLGEPSDLAGPAVFLASDLSKYVTGSQILVDGGLFVNLQ"
gene <1055821..>1058106
/locus_tag="AWJ20_4953"
/db_xref="GeneID:30037141"
mRNA <1055821..>1058106
/locus_tag="AWJ20_4953"
/product="hypothetical protein"
/transcript_id="XM_018882060.1"
/db_xref="GeneID:30037141"
CDS 1055821..1058106
/locus_tag="AWJ20_4953"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736476.1"
/db_xref="GeneID:30037141"
/translation="MASANSRGETLLSSSPSNVISGTSYKGFPGNEQDEDDKDHFHQA
NVSIGSESNRTSRSLTSPVLSQHQLHHDPHVLHDLPSQHDFTYNSHIYSIGQASPQSP
GFSRRSETGSHSSESSLSFTRDTPKRYSKPSLDRRRLSINTLLTNNERLESALELEEM
ECHKLEKTLQDHKIKLSNSLLLQSQLEDDLVKRDEQIGLLQKQAKDARKTRIELETEL
SEEVKKYINEKQMWLSKEEEYEEKLSNCNTKIKSLSENLEVARLEFENLKFSGGSSRS
AAPSVKNVEASQPISDSGANKFIERMKKESEMAQQQMEMVTREYQARYNRIKSEMEQV
QEVNKRLIEENEGFQLLLAEKTILGGFSLADELDQDVNQDVFISEDDETENQQKLSDT
EASSSFESDSDLETPVSKRRPVRNSSLPNYAKDEAVTNAEGVDDIDPLDTDDDNIRSL
DIPSDSSPTSDSKKSESKTPPSLSTNAKRQVYQLQFEKTSLQNYNRALKSSLERLVSR
LLEYREFEKVVEDHNVLNKRSITSFQDRVVSLSNQHPPTLDVPRQRLNSASAPASTAS
NQRGHSFTSSISSGFSSVMGRSKGANSISGPSGNIQGHHSHSSSNPLFHMNPPTTPSG
IVPRPYRGTLRPSSTWSSMLFAASPSTPTSPNINRDSRQFSTTSTNSESTAPRSPSST
SSLVSSSSAASISSGCSGTLSVIDPTILPLSDNKHLVRTTTRSQKQLRPLRLVVAPTD
ANSNPAPPLQSASSQSSGWFS"
gene <1058423..>1060684
/gene="GCN20"
/locus_tag="AWJ20_4954"
/db_xref="GeneID:30037142"
mRNA <1058423..>1060684
/gene="GCN20"
/locus_tag="AWJ20_4954"
/product="putative AAA family ATPase GCN20"
/transcript_id="XM_018882061.1"
/db_xref="GeneID:30037142"
CDS 1058423..1060684
/gene="GCN20"
/locus_tag="AWJ20_4954"
/inference="similar to AA sequence:KEGG_Orthology:K06158"
/note="Positive regulator of the Gcn2p kinase activity;
forms a complex with Gcn1p; proposed to stimulate Gcn2p
activation by an uncharged tRNA; GO_component: GO:0005829
- cytosol [Evidence IPI] [PMID 9234705]; GO_component:
GO:0022626 - cytosolic ribosome [Evidence IDA] [PMID
9234705]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0016887 - ATPase activity
[Evidence IEA]; GO_function: GO:0016887 - ATPase activity
[Evidence ISS] [PMID 7621831]; GO_function: GO:0017111 -
nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_process: GO:0006200 - ATP catabolic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0006448 - regulation of
translational elongation [Evidence IMP,IPI] [PMID
9234705]"
/codon_start=1
/product="putative AAA family ATPase GCN20"
/protein_id="XP_018736477.1"
/db_xref="GeneID:30037142"
/translation="MPSLPDQVRQSVPAIDPIVAEYAAGYVQHISESTSDPVFANNLD
IDGEVEFISSLLKDAGGDPSSVDSLYEKLIEELKKIYAASASKPYIDSASRKLDRDTL
LSAQKHNTNRFGAGGGSDGMDLDLLSGRKVESRVDKKKLEKAEKKIAAKLAKRAAKMT
AVGYEASKLLNIDDKEDYDTFFLTVNPLELGASQGKSKDIKIENFDLYVGAGQRILSN
TSLTLAYGRRYGVVGQNGIGKSTLLKALSRRELAVPKHITILYVEQEVTGDETSALQT
VLDADVWRKHLLLEQKKINDRISEISDLVRPLETTDPDYKALDEERDDLESNLNDIYD
KLTEIESDKAEGRAAAILFGLGFSAEAQQQPTNSFSGGWRMRLSLARALFCKPDLLLL
DEPSNMLDVPSITYLSNYLQSYPSTVVVVSHDRAFLNEVATDIIHQHSERLDYYRGSN
FDSFYATREERRKNQQREYENQMAYRKHLQTFIDKFRYNAAKSSEAQSRIKKLEKLPI
LEPPEEDQTVTFRFPDPDKLSPPVIQLQGVDFGYSPDKLLLKNVDLDVGIDSRIALVG
GNGCGKTTLLKILMDQLKPISGLVSRNGRLRIGYFAQHHIDGMDINLSPVEWMAKMSP
GKSDEECRRLLGAFGITGTLGLQKIALLSGGQKSRVAFACLGLNTPHILVLDEPSNHL
DTAGLDALADALRNFKGGILMVSHDVSTIDRVCNEIWLSESGTVRKFPGSIYDYKKYT
LKQSTGAGVIAKQ"
gene complement(<1060860..>1063883)
/gene="DIS3"
/locus_tag="AWJ20_4955"
/db_xref="GeneID:30037143"
mRNA complement(<1060860..>1063883)
/gene="DIS3"
/locus_tag="AWJ20_4955"
/product="exosome catalytic subunit DIS3"
/transcript_id="XM_018882062.1"
/db_xref="GeneID:30037143"
CDS complement(1060860..1063883)
/gene="DIS3"
/locus_tag="AWJ20_4955"
/inference="similar to AA sequence:KEGG_Orthology:K12585"
/note="Exosome core complex catalytic subunit; has both
endonuclease and 3'-5' exonuclease activity; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; role in degradation of tRNAs; has
similarity to E. coli RNase R and to human DIS3; mutations
in Dis3p corresponding to human mutations implicated in
multiple myeloma cause phenotypes suggesting impaired
exosome function; protein abundance increases in response
to DNA replication stress; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0000177 -
cytoplasmic exosome (RNase complex) [Evidence IDA] [PMID
10465791]; GO_component: GO:0000177 - cytoplasmic exosome
(RNase complex) [Evidence IDA] [PMID 19046973];
GO_component: GO:0000178 - exosome (RNase complex)
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0000176 - nuclear
exosome (RNase complex) [Evidence IDA] [PMID 10465791];
GO_component: GO:0000176 - nuclear exosome (RNase complex)
[Evidence IDA] [PMID 19046973]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 22842922]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 9562621];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22842922]; GO_function: GO:0000175 - 3'-5'-exoribonuclease
activity [Evidence IDA,IMP] [PMID 17173052]; GO_function:
GO:0000175 - 3'-5'-exoribonuclease activity [Evidence
IDA,IMP] [PMID 17643380]; GO_function: GO:0003723 - RNA
binding [Evidence IEA,IEA]; GO_function: GO:0004519 -
endonuclease activity [Evidence IEA]; GO_function:
GO:0004521 - endoribonuclease activity [Evidence IDA,IMP]
[PMID 19060886]; GO_function: GO:0004521 -
endoribonuclease activity [Evidence IDA] [PMID 19060898];
GO_function: GO:0004521 - endoribonuclease activity
[Evidence IDA,IMP] [PMID 19129231]; GO_function:
GO:0004527 - exonuclease activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0004518 - nuclease activity
[Evidence IEA]; GO_function: GO:0000049 - tRNA binding
[Evidence IDA,IMP] [PMID 17643380]; GO_process: GO:0000467
- exonucleolytic trimming to generate mature 3'-end of
5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID 10508172];
GO_process: GO:0000467 - exonucleolytic trimming to
generate mature 3'-end of 5.8S rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 9390555]; GO_process: GO:0043628 - ncRNA 3'-end
processing [Evidence IMP] [PMID 11352936]; GO_process:
GO:0070651 - nonfunctional rRNA decay [Evidence IMP] [PMID
19481524]; GO_process: GO:0071028 - nuclear mRNA
surveillance [Evidence IMP] [PMID 11030620]; GO_process:
GO:0071039 - nuclear polyadenylation-dependent CUT
catabolic process [Evidence IMP] [PMID 15935759];
GO_process: GO:0071042 - nuclear polyadenylation-dependent
mRNA catabolic process [Evidence IC] [PMID 10465791];
GO_process: GO:0071035 - nuclear polyadenylation-dependent
rRNA catabolic process [Evidence IMP] [PMID 16431988];
GO_process: GO:0071035 - nuclear polyadenylation-dependent
rRNA catabolic process [Evidence IMP] [PMID 18940861];
GO_process: GO:0071038 - nuclear polyadenylation-dependent
tRNA catabolic process [Evidence IGI] [PMID 15145828];
GO_process: GO:0071038 - nuclear polyadenylation-dependent
tRNA catabolic process [Evidence IDA] [PMID 15828860];
GO_process: GO:0071038 - nuclear polyadenylation-dependent
tRNA catabolic process [Evidence IDA] [PMID 15935758];
GO_process: GO:0071038 - nuclear polyadenylation-dependent
tRNA catabolic process [Evidence IDA,IMP] [PMID 17643380];
GO_process: GO:0070478 - nuclear-transcribed mRNA
catabolic process, 3'-5' exonucleolytic nonsense-mediated
decay [Evidence IC] [PMID 10465791]; GO_process:
GO:0070481 - nuclear-transcribed mRNA catabolic process,
non-stop decay [Evidence IC] [PMID 10465791]; GO_process:
GO:0071051 - polyadenylation-dependent snoRNA 3'-end
processing [Evidence IC] [PMID 10465791]; GO_process:
GO:0016075 - rRNA catabolic process [Evidence IMP] [PMID
10508172]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]"
/codon_start=1
/product="exosome catalytic subunit DIS3"
/protein_id="XP_018736478.1"
/db_xref="GeneID:30037143"
/translation="MAVATKRQSSSGRGSIAHKVFVRSSKAGNASKIVREQYLRSDIP
CSSEACSVCIPPQNADGTFRPPVLAKIPNSNKQRGPHYVVVDTNIALKAIDLLEHKSI
FYDVIIPQIVLEEVRNRSLPVYNRLRNLTKASDDEKRFYVFHNEFHQDTHVTRNKNES
INDRNDRAIRQTSKWYQDHLKKTVDTRQNISSMDVDGDSSSSSAKMVVVPQIVLISDD
RDNRTKAEAEGITALSLRSYLKEFPNGDDLLDLAPSDDWTATSSGLAAIYPSFYPMSR
LLGGIKNGTLHQGILSISQFNFLEGSVRTQAYPKPILVLGRENLNRGFNGDVVVVELL
PKSQWKIPSSSIIEEEELGKTEGADNESETIETDEERKALAEEALKAQHQQGESAASK
VQPTARIVGIIKRNWRFYVGHVSAQSAAGVSSSDNSQKSIFVVPLDKCIPRIRIKTRK
LGELVNKRIVVSIDDWPIHSRHPEGHFIRSLGEIESKEAETEALLLEHDVEYRPFPKS
VLNCLPPEGHDWKVPENIESTTDEQLKKRRDLRHLLVCSIDPPNCQDIDDALHAHVLP
NGNYEVGVHIADVTHFVKPNTPLDDEGASRGTSVYLVDKRIDMLPMLLGTDLCSLKPY
VDRFSFSVLWEMTPQGEIVNTEFTKSIIRSREAFEYGQAQARIDDESQNDDLTKGMRI
LLDLSKKLKKGRMDAGALNLSSPEVKVNTESETSDPGNVEIKKLLDTNSLVEEFMLLA
NISVAKKIYESYPQTAMLRRHGAPPATNFEILNDQLRIKKGMSIDLESSKTLADSLDK
CVDPEEPFFNNLVRIMATRCMLAAEYFPSGEYGYPEFRHYGLASEIYTHFTSPIRRYA
DVVAHRQLAGAIDYESLHPSHRDRNKMESICRNINKRHRNAQFAGRASIEYYVGQVLK
ADESEQEGFIIKVFSNGINILVPKFGVESLIHLEDLGDPETASYDEDTYTLHLQSKDD
KKPIALSIFDKVKVYVRSVKDETSGKRKVHMSLRS"
gene <1064005..>1065479
/gene="SKI8"
/locus_tag="AWJ20_4956"
/db_xref="GeneID:30037144"
mRNA join(<1064005..1064046,1064506..1064511,1064592..>1065479)
/gene="SKI8"
/locus_tag="AWJ20_4956"
/product="SKI complex subunit WD repeat protein SKI8"
/transcript_id="XM_018882063.1"
/db_xref="GeneID:30037144"
CDS join(1064005..1064046,1064506..1064511,1064592..1065479)
/gene="SKI8"
/locus_tag="AWJ20_4956"
/inference="similar to AA sequence:KEGG_Orthology:K12601"
/note="Ski complex component and WD-repeat protein;
mediates 3'-5' RNA degradation by the cytoplasmic exosome;
also required for meiotic double-strand break
recombination; null mutants have superkiller phenotype;
GO_component: GO:0055087 - Ski complex [Evidence IDA]
[PMID 10744028]; GO_component: GO:0055087 - Ski complex
[Evidence IDA,IPI] [PMID 18042677]; GO_component:
GO:0005694 - chromosome [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0000228 - nuclear chromosome [Evidence IDA] [PMID
14992724]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0051607 - defense response
to virus [Evidence IEA]; GO_process: GO:0007126 - meiotic
nuclear division [Evidence IEA]; GO_process: GO:0070478 -
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [Evidence IGI,IMP]
[PMID 12881429]; GO_process: GO:0034427 -
nuclear-transcribed mRNA catabolic process,
exonucleolytic, 3'-5' [Evidence IMP] [PMID 9482746];
GO_process: GO:0070481 - nuclear-transcribed mRNA
catabolic process, non-stop decay [Evidence IMP] [PMID
11910110]; GO_process: GO:0070481 - nuclear-transcribed
mRNA catabolic process, non-stop decay [Evidence IMP]
[PMID 17660569]; GO_process: GO:0006461 - protein complex
assembly [Evidence IMP] [PMID 14992724]; GO_process:
GO:0065004 - protein-DNA complex assembly [Evidence IMP]
[PMID 14992724]; GO_process: GO:0007131 - reciprocal
meiotic recombination [Evidence IGI,IMP,IPI] [PMID
14992724]"
/codon_start=1
/product="SKI complex subunit WD repeat protein SKI8"
/protein_id="XP_018736479.1"
/db_xref="GeneID:30037144"
/translation="MKINFSTAARLLHELTILLWNNKDPEPKSAVKLNSPSSGTHHLS
TDRSGRWLVAVSFDGKATLYDLDNLAGENGGSGRLLFEDVWAVAIEPNARHVVASQIT
GDIAVWDISAITEETPDQTTESAASQPQIKRIQKFSGNGGVLGLSVDFASNEEHVAAG
FESGAIHVYNYKTGRLEYSLTGHSNPIRTVKFSPAATLLAIGGDSKLISLYSMTSGEH
ITNLTGSDGWVFGLDWNETGEFLLSASYDGRSRVWWLESRQCVAAQNDSSQPLFGCKW
LKKGWGVGVIGGINQGFATVGVDKTLRWYREAAGN"
gene complement(<1066075..>1069014)
/gene="PRP6"
/locus_tag="AWJ20_4957"
/db_xref="GeneID:30037145"
mRNA complement(<1066075..>1069014)
/gene="PRP6"
/locus_tag="AWJ20_4957"
/product="Prp6p"
/transcript_id="XM_018882064.1"
/db_xref="GeneID:30037145"
CDS complement(1066075..1069014)
/gene="PRP6"
/locus_tag="AWJ20_4957"
/inference="similar to AA sequence:KEGG_Orthology:K12855"
/note="Splicing factor; component of the U4/U6-U5 snRNP
complex; GO_component: GO:0046540 - U4/U6 x U5 tri-snRNP
complex [Evidence IDA] [PMID 10377396]; GO_component:
GO:0046540 - U4/U6 x U5 tri-snRNP complex [Evidence IDA]
[PMID 10449419]; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA,IEA]; GO_component: GO:0005681 -
spliceosomal complex [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0006396 - RNA processing [Evidence IEA]; GO_process:
GO:0008380 - RNA splicing [Evidence IEA]; GO_process:
GO:0006397 - mRNA processing [Evidence IEA]; GO_process:
GO:0000398 - mRNA splicing, via spliceosome [Evidence
IEA]; GO_process: GO:0000398 - mRNA splicing, via
spliceosome [Evidence IPI] [PMID 10377396]"
/codon_start=1
/product="Prp6p"
/protein_id="XP_018736480.1"
/db_xref="GeneID:30037145"
/translation="MSGNKLSFLDMPAPAGYVPGLGRGATGFTTRSDIGPAKAGIVGD
AVRAGEGDGNVVDDEERFRDPENDSGLLRSGFLDADDAEADRIYEAIDQRMEERRRQQ
REAREREEQEEFERKNPKISTQFKDLKRALGTISADEWASLPEVGDLTRKNKRQRKDM
ERRFYALPDSVIMGARNSGAVDSSIEAGGAAGLDETDETITDFRSISLAKDKMLGLRL
DQAGTTAVPGSLTNGNGVGSVLNTSNNELVNGTENGRQTSVQRQPVYGGASTIDAKSY
LSLSASAGVSGAEIGDIRRVRPLLESLVKTDPKEPRGWIGLARLEEIAHRMNKARSVI
QQGCDNCPRNEDVWLENIRLHDKRTGKIIAAKAVGMIPNSINLWLAASDLEDDVNAKK
RVIQKALESNPTSDTLWKKAVNLESEPNDAKLLLAQAVELVPLAEDLWLALARIETYK
NARKVLGKARKALRVSRAVWIAAARLEEQEKGDAKTVDNIMS 