LOCUS NC_031674 4956242 bp DNA linear CON 25-OCT-2016
DEFINITION Sugiyamaella lignohabitans strain CBS 10342 chromosome B, complete
sequence.
ACCESSION NC_031674
VERSION NC_031674.1
DBLINK BioProject: PRJNA342695
BioSample: SAMN04417247
Assembly: GCF_001640025.1
KEYWORDS RefSeq.
SOURCE Sugiyamaella lignohabitans
ORGANISM Sugiyamaella lignohabitans
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Trichomonascaceae;
Sugiyamaella.
REFERENCE 1 (bases 1 to 4956242)
AUTHORS Bellasio,M., Peymann,A., Valli,M., Sipitzky,M., Graf,A., Sauer,M.,
Marx,H. and Mattanovich,D.
TITLE Complete genome sequence and transcriptome regulation of the
pentose utilising yeast Sugiyamaella lignohabitans
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4956242)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (25-OCT-2016) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 4956242)
AUTHORS Peymann,A. and Graf,A.
TITLE Direct Submission
JOURNAL Submitted (18-FEB-2016) Department of Biotechnology, University of
Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna
1190, Austria
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to CP014503.
##Genome-Annotation-Data-START##
Annotation Provider :: INSDC submitter
Annotation Status :: Full annotation
Annotation Pipeline :: Eukaryotic Annotation Propagation Pipeline
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..4956242
/organism="Sugiyamaella lignohabitans"
/mol_type="genomic DNA"
/strain="CBS 10342"
/type_material="culture from holotype of Sugiyamaella
lignohabitans"
/db_xref="taxon:796027"
/chromosome="B"
gene <2139..>2819
/gene="SDO1"
/locus_tag="AWJ20_1962"
/db_xref="GeneID:30033821"
mRNA <2139..>2819
/gene="SDO1"
/locus_tag="AWJ20_1962"
/product="guanine nucleotide exchange factor SDO1"
/transcript_id="XM_018878881.1"
/db_xref="GeneID:30033821"
CDS 2139..2819
/gene="SDO1"
/locus_tag="AWJ20_1962"
/inference="similar to AA sequence:KEGG_Orthology:K14574"
/codon_start=1
/product="guanine nucleotide exchange factor SDO1"
/protein_id="XP_018736851.1"
/db_xref="GeneID:30033821"
/translation="MLTSGSSSHNSHSNIDREKDLDEVLQIPQVFVHVSKGQVANNED
LKAAFGTTDQNVIIQEILKKGELQVGGKEREALSNQMHAEIIQLIAAKCVNPGTKRPY
PTSIIDKALSESNFNMVPNKSAKIQALDAIKLLMQSNIIPIVRARMKVLIRVDSSKDA
KKFSDKVKALTAEIDDEDWSGKWELTAFIDPGNFRILDELIQKETKGRGSVEVLDTAV
VKEGDQDL"
gene complement(<2859..>3389)
/locus_tag="AWJ20_1963"
/db_xref="GeneID:30033822"
mRNA complement(<2859..>3389)
/locus_tag="AWJ20_1963"
/product="hypothetical protein"
/transcript_id="XM_018878882.1"
/db_xref="GeneID:30033822"
CDS complement(2859..3389)
/locus_tag="AWJ20_1963"
/inference="similar to AA sequence:KEGG_Orthology:K11877"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736852.1"
/db_xref="GeneID:30033822"
/translation="MKFDDLQDLAGTLHNMSESNDKIEFPAASKTKSIELQDGHIIDV
VTVGFEDKISVMVNYDGRIGKIYYVPLLASSTSHLANYENRDDDGNPENDLLPLSHLT
PMPLVGGSSDHDVQGRLYASMIASIVSRQSPDENRTVLVGLGTSIGPRPDEEITIEHR
KQLLEIVKLVQECRVW"
gene complement(<4700..>5089)
/locus_tag="AWJ20_1964"
/db_xref="GeneID:30033823"
mRNA complement(<4700..>5089)
/locus_tag="AWJ20_1964"
/product="hypothetical protein"
/transcript_id="XM_018878883.1"
/db_xref="GeneID:30033823"
CDS complement(4700..5089)
/locus_tag="AWJ20_1964"
/inference="similar to AA sequence:KEGG_Orthology:K08968"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736853.1"
/db_xref="GeneID:30033823"
/translation="MYKSLESSPLSKVNWAGFYVVDPKDSSQLILGPFHGQVACQIIK
IGKGVCGTAASSRETQLVKDVEKFPGHIACDGDTKSEIVVPIVDKSSGKVFGVIDIDC
QELEGFDEEDQKYLEILAEKLIKYCKW"
gene complement(<5690..>9004)
/gene="MSS4"
/locus_tag="AWJ20_1965"
/db_xref="GeneID:30033824"
mRNA complement(<5690..>9004)
/gene="MSS4"
/locus_tag="AWJ20_1965"
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/transcript_id="XM_018878884.1"
/db_xref="GeneID:30033824"
CDS complement(5690..9004)
/gene="MSS4"
/locus_tag="AWJ20_1965"
/inference="similar to AA sequence:KEGG_Orthology:K00889"
/note="Phosphatidylinositol-4-phosphate 5-kinase; involved
in actin cytoskeleton organization and cell morphogenesis;
multicopy suppressor of stt4 mutation; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 9624177];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IEA]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624177]; GO_function: GO:0016308 -
1-phosphatidylinositol-4-phosphate 5-kinase activity
[Evidence IDA] [PMID 9624178]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0016301 -
kinase activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016307
- phosphatidylinositol phosphate kinase activity [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0031321 - ascospore-type
prospore assembly [Evidence IGI] [PMID 19502581];
GO_process: GO:0046488 - phosphatidylinositol metabolic
process [Evidence IEA]; GO_process: GO:0046854 -
phosphatidylinositol phosphorylation [Evidence IDA] [PMID
9624177]; GO_process: GO:0046854 - phosphatidylinositol
phosphorylation [Evidence IDA] [PMID 9624178]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="1-phosphatidylinositol-4-phosphate 5-kinase"
/protein_id="XP_018736854.1"
/db_xref="GeneID:30033824"
/translation="MLAASSPSVTQLQLPFSYQRHSNEAFESEKSRQHSSGTSTSTDT
SGGASVSGSDDEHKLNSKLSIVTNGGTLVADNEHQSSDDIDDAFLSHNSLSARSMSSL
ATTVDSRDYEKSISTNIAVHSAPLDGQMTSSASSINGLSTTFLPVGDFHVDGPVNGTD
DVSSLDNNTVEIETDGTRGFDNDDASYARGQRFGSDTFYDAPNSNNTSSSLTADSLLT
VTDNADHREQPASQPHESSQLASYKGLRVSTKGDLPTLSCAPEVVANELSTVNSEERS
VNEKPSENGNSIEHEGSTAHGEFLTAPTMDSAEATSGTTSTTSTSSTPTSISNSSTAT
SDSSVTTPVVSATPLPFTPPQPNYHSPKSQSASTFTPTSTSVSKPARNDSSSNSGPNQ
SPLTTQASNARTSSTSSVATANVLANSSSTSPVPRQMPTFRGSLPGIESSPQLLDPIP
SHVRTSIGSTPPSPNVRTSVGSAFSFDDQRYLSRASTGSHHSIASPTSPVSAQQLASL
RSPNPSASSSSSLPSMVRERVSNAGSVAVPNVNSTGAVAMTGSTIATANIAPTTLMGP
PQLPTRALSQQIVSTSNQSNNRNSLSSQRSAASSQEVPSIRRLSLYSHRNSFSFSRRR
SIAGPPIPSNGQELDRSATVIEIERLREEIIVQRELRRRRREFLEDERVLMGTKVSEG
HVNYVTAYNMLTGIRVSVSRCNGKVDRPLNDEDFVAKNKLAFDVSGNELTPSARYDFK
FKDYAPWVFRHLRELFKLDPADYLMSLTSKYIVSELGSPGKSGSFFYFSRDYRFIIKT
IHHSEHKMLRKILKDYYNHVKNNPNTLISQFYGLHRVKLPFGRKIHFIVMNNLFPPHR
DIHRTYDLKGSTQGRRLQPQDSKKAVVYKDLNWIDAGESIMLGPTKKRQFLDQLEIDV
ALLKRLNIMDYSLLIGIHDLTKGNTENIRDNTLQVFEPGSATISQTKVNELRKALTTA
SPTALAQLDLVLNQYERNDFIFYADSGGFRATNENNEPLNDIYYLGIIDCLTPYTFTK
RVETFFKGLSNAHVTISAIPALEYGDRFFKFIKSVVVSKREGQQNVIGEETAPSAIEV
HLPVLSEASTEARSVN"
gene complement(<11084..>13663)
/locus_tag="AWJ20_1966"
/db_xref="GeneID:30033825"
mRNA complement(<11084..>13663)
/locus_tag="AWJ20_1966"
/product="hypothetical protein"
/transcript_id="XM_018878885.1"
/db_xref="GeneID:30033825"
CDS complement(11084..13663)
/locus_tag="AWJ20_1966"
/inference="similar to AA sequence:KEGG_Orthology:K01192"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736855.1"
/db_xref="GeneID:30033825"
/translation="MTSGPVKPTHSTTLANWSYRKAGTEKWTASKHGRPATQIHVDLL
DNGEIPDPFLDENEKLVQWVADEEWEYQAEFTVSSEQLESGKKTDFVYEGLDTVATVY
LNDKQILHSENMFHIGRVDITKDVKVGTNNVRIFFANALIRSRQLEKIHGKMRLFNGE
TSRVQLRKSQYHYGWDWGPLLMTSGPYRPVKIETYNYLVSDVFVNAKVSDDLKEVTVS
IEGEIEGSKGEPGYLPLQFKVDIKSPSGEQVVNKTFDISVDKDGVSKNSLKIENPQLW
YPANYGEQHLYHITVDVVSGSETIQSIEKDVGFRKVELIQRPLVDQEGTTFFFQVNNI
PIYCAGGCWIPAHSFTTCLTNDDYKKWIKLMIDGHQNMVRIWGGGIYEEDIFYQECDR
LGIMVWQDFMFACGQYPGYPEFLESVSKEAVDQLKRLRNYCSIVLYAGNNEDYQVAEA
YPEIEYDPNDTTSDYLKTNFPARHIYEKMFPELVADYSPAVPYHPGSPWGGKTSSDPT
IGDIHQWNVWHGNQEKYQDWYKLGGRFVSEFGMEALPDRKTVDQFVTKEHEKYPQSRT
MDLHNKSDGFERRLALYVMENIKVEGMDIDNWIYATQLMQSECLAYAYRCWRRQWKGP
GREYCAGALVWQVNDCWPVTSWAIVDFYYRPKLSYYAVKRESSPVWIGMYRNVNYLSE
EDKKAAEEVPTFDYFKREHFEDIWGVNNTIHEKKVVLHVEVYEIESGKLVETLPPKPV
TLKANQTTEIIQNLPVESKTHHVVYCRLVDADSNKVIARGGDWPQPLKYLSFPDRQVK
VKVEDGQVRLSTNKPVKGVEITVKNKDLFLEDNGIDLFPGDDQTVIIRGLLSTDEVTI
RHY"
gene <15080..>16726
/locus_tag="AWJ20_1967"
/db_xref="GeneID:30033826"
mRNA <15080..>16726
/locus_tag="AWJ20_1967"
/product="hypothetical protein"
/transcript_id="XM_018878886.1"
/db_xref="GeneID:30033826"
CDS 15080..16726
/locus_tag="AWJ20_1967"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736856.1"
/db_xref="GeneID:30033826"
/translation="MSDQRSGNEHERSPLLAYSPNDESAPHPLPRSYTISEGQDENGI
LMNSGNHHETVNDYNPSSDSSRRSSSKLNFNDVNFGNNPFKKRHFSYSAGGTSPSRHH
SFYSQLQQHGSRISLGDAAALELDVERLENLHERRPFRLIGKSCPLRDWSRYCKTEEQ
IRQISRAKAIQEYYSKQNDLIERYKAIDRLLDSGIPQSMLKVYGDDIDLGAPNGTTSK
KADDPPSPPGNRQGVPANIDEESNLLGGESREDKSNMVMFAIYVNFVINFVLLVGKVI
VVLLTNSLSVVASLVDSILDFLSTFIIWASTRLVDQRDWKTKHLYPVGRSRLEPIGVL
VFSILIIVSFLKVGDEAINKLLEGRQGSAVDIGIPSIIIMSLTIVVKVFCYLWCRTID
SSAVQALAQDAMTDIVFNTFSMLFPLVGHAYDIWWLDPFGALALSAYIIHSWASTAME
HIDNLTGSAASPEDRQVVLYLCMRFSDSIKYITALNAYHAGDRITVEVDIVLDDKYNL
RDCHDIAEALQYAIETLPFVERAFVHLDYRRGNFTGHIDR"
gene <19208..>20860
/gene="NAM8"
/locus_tag="AWJ20_1968"
/db_xref="GeneID:30033827"
mRNA <19208..>20860
/gene="NAM8"
/locus_tag="AWJ20_1968"
/product="Nam8p"
/transcript_id="XM_018878887.1"
/db_xref="GeneID:30033827"
CDS 19208..20860
/gene="NAM8"
/locus_tag="AWJ20_1968"
/note="RNA binding protein, component of the U1 snRNP
protein; mutants are defective in meiotic recombination
and in formation of viable spores, involved in the
formation of DSBs through meiosis-specific splicing of
REC107 pre-mRNA; Nam8p regulon embraces the meiotic
pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the
putative RNA binding domains RRM2 and RRM3 are required
for Nam8p meiotic function; GO_component: GO:0005685 - U1
snRNP [Evidence IDA] [PMID 9630245]; GO_component:
GO:0071004 - U2-type prespliceosome [Evidence IDA] [PMID
16618970]; GO_component: GO:0000243 - commitment complex
[Evidence IPI] [PMID 10072386]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 23222640]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
23222640]; GO_function: GO:0003723 - RNA binding [Evidence
IEA]; GO_function: GO:0003723 - RNA binding [Evidence IPI]
[PMID 10072385]; GO_function: GO:0003729 - mRNA binding
[Evidence IPI] [PMID 10072386]; GO_function: GO:0003729 -
mRNA binding [Evidence IDA] [PMID 23222640]; GO_function:
GO:0003676 - nucleic acid binding [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_process: GO:0006397 - mRNA processing [Evidence
IEA]; GO_process: GO:0006376 - mRNA splice site selection
[Evidence IMP] [PMID 10072385]; GO_process: GO:0000398 -
mRNA splicing, via spliceosome [Evidence IPI] [PMID
10072386]; GO_process: GO:0048026 - positive regulation of
mRNA splicing, via spliceosome [Evidence IMP] [PMID
10983980]"
/codon_start=1
/product="Nam8p"
/protein_id="XP_018736857.1"
/db_xref="GeneID:30033827"
/translation="MSFSYDYSEAQAQQQQQQQSQPQAADISVSNSGEPKVEPSSVTT
DASAAGTAGSASSATTTGAAGSVGLDANGAGSVVPPGTSTSPALVAGSPPTASSDSKS
TLWMGELEPWYDENAIGKIWQNFGENVTVKLIRDKYSGVNAGYCFVDFGTPQAAAKAL
SLNGQPIPNSQRVFRLNWASGGGLIDKRDDRGPEFSIFVGDLSPEVTEFNLLQLFQSR
YSSCKSAKIMTDPSTNASRGYGFVRFNDETHMQRALAEMQGFFLGNRPIRISTATPKS
KPGQNGLVGNSGNGAGPNVGGPGGLYHQMGMGGPPPPAGYYGTPQTLNQFTDPNNTTV
FVGGLSGHVTEDELRRYFAGFGDITYVRIPPGKGCGFVQFVQRHSAEAAITQMQGYPI
GSSRVRLSWGRSQNAVANAGLNGLPGGPGGNNGPPPGAYRPAPPPPNVYPQLNIPAQP
HYGPYGPINPHVQGQVDIHGQQQQQLGGPIPGVAGAAGAAGPVPGVAQVNNGADPLLP
LGQDPSEPVPVARLNELYLAARDGRLDRMDADGRGYHGVYAQ"
gene <23364..>27506
/gene="SWR1"
/locus_tag="AWJ20_1969"
/db_xref="GeneID:30033828"
mRNA <23364..>27506
/gene="SWR1"
/locus_tag="AWJ20_1969"
/product="chromatin-remodeling protein SWR1"
/transcript_id="XM_018878888.1"
/db_xref="GeneID:30033828"
CDS 23364..27506
/gene="SWR1"
/locus_tag="AWJ20_1969"
/inference="similar to AA sequence:KEGG_Orthology:K11681"
/codon_start=1
/product="chromatin-remodeling protein SWR1"
/protein_id="XP_018736858.1"
/db_xref="GeneID:30033828"
/translation="MKKGKTPSKSTPKDSTNASSSRKKSQKDNNDDESQSESSFDSDD
DSEVLYNDFGERINIPSYESDFATDDLSSGDEEKIPRLKFKFSVPNQTVTHPSHLSNP
KFGGSLEKYLDSFISLDDDITLEERDSFVDDQVEVRNRIERAKKSGLLQRHTHGLATN
IEKFADPPSFDISNNHHSHLVSHAIYFARLMTEERKAHISRARRIANIVDLHFKRLSG
AEEKERKQFERSMRQLARRTGAEVMKKWKVAEKVVRQRRQQALEEQQRNEGKLQLNQI
LKQSAQLLEARTEKMTASVNVDDLSDATDENESAGSLADSDDESDAGGSESSDNDSEV
MSSSDDDSEVEDVDDEKLTVEQLKEKYSKLKSVTFNDLENKVDVDIIESEDEDEEETV
EIDEKAKSLYEEEQSGDSDESVAMDSEFDSDEDDSDESEEASDEEDEPASLASLFNKP
QSNDDKQVEADDEQDEYKEPEIEVDIEANQEDQDSDASDKSIKHSSPDTPTSTPEHEH
GEIADGTVTTTSHIPTPFLLRGKLREYQSDGLDWLAGLYNTDTNGILADEMGLGKTIQ
TISLISYLACEKHIWGPHLIVVPTSVMLNWEMEFKRFAPGFKVMTYYGNPNQRKEKRK
GWNKENTWHVCITSYQLVLQDRNVFKRKKWHYMILDEAHNIKNFRSQRWQALLNFNSE
RRLLLTGTPLQNNLMELWSLLYFLMPSSKGSAMLPDGFANLKDFQEWFARPVDKLVEG
GGQDADDETRATVLKLHQILRPYLLRRLKADVEKQMPAKYEHIVYCRLSKRQRYLYDD
FMSRAQTRETLSSGNFLSIINCLMQLRKVCNHPDLFEVRPIVTSLAIGKSVVSNFEPL
DIIVRKRLLRESYEDHVNLLTLNLLPIKNEGSSFVDWQTIQQLQAYTPMVNELNELVT
QLKTSPVQPNYVSIEGNTKYQEYRAKQSRCDKLQHSIYLNFFRCSRKPIYGNDLVSAV
TLQNHRPRRSGFDWRQSDALSEMKLSYEQRDEQMKEIITRYAFVTPKVVCLNMTRLTL
APVVEEMQSPENQLLLSRQQTFHEAQVKLSIAFPDKRLLQYDCGKLQRLSTLMRDLID
GGHRALIFTQMTKVLDILEQFLNIHGWRYLRLDGATKVEQRQVLTERFNTDARIPVFI
LSTRSGGLGINLTGADTVIFYDSDWNPSMDKQCQDRCHRIGQTRDVHIYRFVSEYTIE
SNILRKATQKQILDNVVIQEGDFTTDYFHKMSVKELVGDVAGVDLDHDLAEPPQNSRS
LAKALAQAEDADDAVAASVAMREANLDAEDFSERATSTAATPGRSESEQPTGVSEQSV
EPPTGASVEPTDGSVEPSVEPDALQSEQEPDLSDSEESDDGIGHIDDYMIKFIEAGYF
GD"
gene <31192..>33165
/gene="PDE2"
/locus_tag="AWJ20_1970"
/db_xref="GeneID:30033830"
mRNA <31192..>33165
/gene="PDE2"
/locus_tag="AWJ20_1970"
/product="3',5'-cyclic-nucleotide phosphodiesterase PDE2"
/transcript_id="XM_018878890.1"
/db_xref="GeneID:30033830"
CDS 31192..33165
/gene="PDE2"
/locus_tag="AWJ20_1970"
/inference="similar to AA sequence:KEGG_Orthology:K01120"
/note="High-affinity cyclic AMP phosphodiesterase;
component of the cAMP-dependent protein kinase signaling
system, protects the cell from extracellular cAMP,
contains readthrough motif surrounding termination codon;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0004115 -
3',5'-cyclic-AMP phosphodiesterase activity [Evidence IDA]
[PMID 3025832]; GO_function: GO:0004114 -
3',5'-cyclic-nucleotide phosphodiesterase activity
[Evidence IEA,IEA]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_function:
GO:0008081 - phosphoric diester hydrolase activity
[Evidence IEA]; GO_process: GO:0019933 - cAMP-mediated
signaling [Evidence IMP] [PMID 8391474]; GO_process:
GO:0008152 - metabolic process [Evidence IEA,IEA,IEA];
GO_process: GO:0007165 - signal transduction [Evidence
IEA]"
/codon_start=1
/product="3',5'-cyclic-nucleotide phosphodiesterase PDE2"
/protein_id="XP_018736859.1"
/db_xref="GeneID:30033830"
/translation="MLGPAGKPIEPPSSPTNPLRKFTGKVAQVASQLSQKSMPRLRPS
TSTRHLNNSTSTRETTTSGSISTSTYREASSVTNTNRNSKLHNPSMASKTSSPALSVN
SFSSTSAPSLVLYWDVRVPKDNQIYDRFSKLLDNDVSRSKYPEVYSNISLLLEQFPQV
HVLNTFQSLAQQLFAYRECAFTTLFYVTTSDLVYVNDDQTIKFVSAQYAHIPGAFIVL
SDVTSLWASFARLGAMDISWASIESVKATKPAYWASIIGHARQDQFESRTRQLKTMLG
LEQEVESVASANLITKFSFSSKHVLDEFSPSLTSLVNEEKQPLIQELIGSWDFYAHDL
KYDELLAATYMMIKHALSSPGLEHYCIPDRKLISLLLVVRNCYHATNPYHNFRHAVDV
VQATFFFLISIGVFKPVGKAASVEKSTNPLTAVDGLNLLVAALGHDVGHPGVTNAFLV
SSNAPLAKIYNDRSVLESYHSAAFNQILKEYWPQMVQDSNARTMITESVLATDMAVHF
DYMKKAEEKGKQDSLYDENINENELQKLRLLLGCLILKCADISNVSRKLDISSKWGTL
LVAEFGGLNDLEIDLGLKQAPSQSNEPVTAGLRNKLLGEGQIFFIETYALPLFTAVSR
MMPELQFATRQIEENVKIWKSRVGDGDYDNDHK"
gene complement(<33355..>35247)
/gene="VTS1"
/locus_tag="AWJ20_1971"
/db_xref="GeneID:30033831"
mRNA complement(<33355..>35247)
/gene="VTS1"
/locus_tag="AWJ20_1971"
/product="Vts1p"
/transcript_id="XM_018878891.1"
/db_xref="GeneID:30033831"
CDS complement(33355..35247)
/gene="VTS1"
/locus_tag="AWJ20_1971"
/note="Flap-structured DNA-binding and RNA-binding
protein; stimulates deadenylation-dependent mRNA
degradation mediated by the CCR4-NOT deadenylase complex;
member of the Smaug (Smg) family of post-transcriptional
regulators which bind RNA through a conserved sterile
alpha motif (SAM) domain that interacts with Smg
recognition element (SREs) containing transcripts;
stimulates Dna2p endonuclease activity; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0000932 - cytoplasmic mRNA processing body [Evidence
IDA] [PMID 18469165]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 11445562]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 20007605]; GO_function:
GO:0003723 - RNA binding [Evidence IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IDA] [PMID 12858164];
GO_function: GO:0003723 - RNA binding [Evidence IDA] [PMID
16429156]; GO_function: GO:0070336 - flap-structured DNA
binding [Evidence IDA] [PMID 20007605]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0000956 - nuclear-transcribed mRNA
catabolic process [Evidence IMP] [PMID 12858164];
GO_process: GO:0000956 - nuclear-transcribed mRNA
catabolic process [Evidence IMP] [PMID 16429156];
GO_process: GO:0000289 - nuclear-transcribed mRNA poly(A)
tail shortening [Evidence IMP,IPI] [PMID 18469165];
GO_process: GO:0032079 - positive regulation of
endodeoxyribonuclease activity [Evidence IDA,IPI] [PMID
20007605]; GO_process: GO:0015031 - protein transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Vts1p"
/protein_id="XP_018736860.1"
/db_xref="GeneID:30033831"
/translation="MEDFSYASNPGNNNMSSRIQQPSHRMHQQVNSSSSLPPFDSPLA
ARTRPTSEIFLQTPLKQFGTSNGMNTIGPGGVSLGPGSGTNSALANHINPDDQMMDLA
AEKWLADLELYQKTLEEMAEVSLDKNFKEELNDIERWFEVLSVGERTAALYALLQQTT
PVQIRFFITVLQKIANRDPISAVLSPTNLEREPLQSQLNEAITRQQQGQQSNSSTSPS
NKNISVSNGSTSSKGQSQHHLHQPQPHSAGFTQNYPHLHSPIPSGVGTSPFMTPPPKH
DLYNNSFLGSFSKPIAPPISPWAAQEINRPKSADAPTGQNSQLHSLIQQQQLQGLQGF
RQFGGAGSRLSNLSVTSNSSDTPSVGGGNSNTYKHNKSLSVLDDSLSSYLPDSSTNWS
SIVNTPVSHIGTPQSKLNSEIINSTAMKMAALSTVNNRVVLDSDVKKFRRRGSLISNE
DQKYIDRELQNLHIQANKHTSTNSPFASTPSPASWTLGISNNTTSTTNNTNNHNIGNG
SPMPSNKHNISTSPQDRKSGLSKVSYGHQDSPSRDMTTSPAPLGTPKKDAPVDIELLQ
DIGAWLRSLRLHKYTDNLSDLNWKDLVELTDADLEKRGVNALGARRKMLKVFEQVKEA
QQVGSI"
gene complement(<37188..>37670)
/gene="RTT106"
/locus_tag="AWJ20_1972"
/db_xref="GeneID:30033832"
mRNA complement(<37188..>37670)
/gene="RTT106"
/locus_tag="AWJ20_1972"
/product="Rtt106p"
/transcript_id="XM_018878892.1"
/db_xref="GeneID:30033832"
CDS complement(37188..37670)
/gene="RTT106"
/locus_tag="AWJ20_1972"
/note="Histone chaperone; involved in regulation of
chromatin structure in both transcribed and silenced
chromosomal regions; affects transcriptional elongation;
has a role in regulation of Ty1 transposition; interacts
physically and functionally with Chromatin Assembly
Factor-1 (CAF-1); GO_component: GO:0005694 - chromosome
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0003690 -
double-stranded DNA binding [Evidence IDA,IMP] [PMID
20007951]; GO_function: GO:0042393 - histone binding
[Evidence IDA,IPI] [PMID 16157874]; GO_function:
GO:0042393 - histone binding [Evidence IDA] [PMID
18662540]; GO_function: GO:0042393 - histone binding
[Evidence IDA,IMP] [PMID 20007951]; GO_process: GO:0006335
- DNA replication-dependent nucleosome assembly [Evidence
IGI] [PMID 18662540]; GO_process: GO:0006336 - DNA
replication-independent nucleosome assembly [Evidence IMP]
[PMID 18708354]; GO_process: GO:0070869 - heterochromatin
assembly involved in chromatin silencing [Evidence
IGI,IMP] [PMID 17410207]; GO_process: GO:0070869 -
heterochromatin assembly involved in chromatin silencing
[Evidence IGI] [PMID 20007951]; GO_process: GO:0000122 -
negative regulation of transcription from RNA polymerase
II promoter [Evidence IMP] [PMID 19683497]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006368 - transcription
elongation from RNA polymerase II promoter [Evidence
IGI,IMP] [PMID 18708354]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0032196 - transposition [Evidence IEA]"
/codon_start=1
/product="Rtt106p"
/protein_id="XP_018736861.1"
/db_xref="GeneID:30033832"
/translation="MLLFPVDKIAAISYSSITRLTFNLNIKLTEPIEGIQDYEFSMID
HNDYDEINDYVVRNSLENQSMSEARRAKQATNKPEFSNELEKVIAEDNGAGDDDEDEE
QDEDYESGSDSEEAASESESDDDDQDNDEDDSDEEEEEEEEDDDEGGQESGQEQDDAE
"
gene complement(<41941..>42411)
/gene="GLC7"
/locus_tag="AWJ20_1973"
/db_xref="GeneID:30033833"
mRNA complement(<41941..>42411)
/gene="GLC7"
/locus_tag="AWJ20_1973"
/product="type 1 serine/threonine-protein phosphatase
catalytic subunit GLC7"
/transcript_id="XM_018878893.1"
/db_xref="GeneID:30033833"
CDS complement(41941..42411)
/gene="GLC7"
/locus_tag="AWJ20_1973"
/inference="similar to AA sequence:KEGG_Orthology:K06269"
/note="Type 1 serine/threonine protein phosphatase
catalytic subunit; cleavage and polyadenylation factor
(CPF) component; involved in various processes including
glycogen metabolism, sporulation, mitosis; accumulates at
mating projections by interaction with Afr1p; interacts
with many regulatory subunits; involved in regulation of
the nucleocytoplasmic shuttling of Hxk2p; import into
nucleus is inhibited during spindle assembly checkpoint
arrest; GO_component: GO:0005935 - cellular bud neck
[Evidence IDA] [PMID 11412094]; GO_component: GO:0000778 -
condensed nuclear chromosome kinetochore [Evidence IDA]
[PMID 19948764]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005847 - mRNA
cleavage and polyadenylation specificity factor complex
[Evidence IDA] [PMID 12819204]; GO_component: GO:0001400 -
mating projection base [Evidence IDA] [PMID 10747092];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
11412094]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 23418575]; GO_component: GO:0000164 - protein
phosphatase type 1 complex [Evidence IDA] [PMID 8289829];
GO_component: GO:0005816 - spindle pole body [Evidence
IDA] [PMID 10747092]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA,IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0004721
- phosphoprotein phosphatase activity [Evidence IEA,IEA];
GO_function: GO:0004722 - protein serine/threonine
phosphatase activity [Evidence IDA] [PMID 1660885];
GO_process: GO:0000077 - DNA damage checkpoint [Evidence
IMP] [PMID 19884341]; GO_process: GO:0000076 - DNA
replication checkpoint [Evidence IGI,IMP] [PMID 19884341];
GO_process: GO:0030437 - ascospore formation [Evidence
IMP] [PMID 9584086]; GO_process: GO:0005975 - carbohydrate
metabolic process [Evidence IEA]; GO_process: GO:0007114 -
cell budding [Evidence IMP] [PMID 10639337]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0051301 - cell division [Evidence IEA]; GO_process:
GO:0006873 - cellular ion homeostasis [Evidence IMP] [PMID
11973298]; GO_process: GO:0007059 - chromosome segregation
[Evidence IMP] [PMID 10072383]; GO_process: GO:0070940 -
dephosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 24413056]; GO_process: GO:0005977 -
glycogen metabolic process [Evidence IEA]; GO_process:
GO:0005977 - glycogen metabolic process [Evidence IMP]
[PMID 1660885]; GO_process: GO:0016576 - histone
dephosphorylation [Evidence IMP] [PMID 10975519];
GO_process: GO:0016576 - histone dephosphorylation
[Evidence IDA,IMP] [PMID 19884341]; GO_process: GO:0006397
- mRNA processing [Evidence IEA]; GO_process: GO:0007126 -
meiotic nuclear division [Evidence IPI] [PMID 8754819];
GO_process: GO:0007067 - mitotic nuclear division
[Evidence IEA]; GO_process: GO:0007094 - mitotic spindle
assembly checkpoint [Evidence IMP] [PMID 10072380];
GO_process: GO:0035307 - positive regulation of protein
dephosphorylation [Evidence IMP] [PMID 23418575];
GO_process: GO:0006470 - protein dephosphorylation
[Evidence IDA] [PMID 1660885]; GO_process: GO:0034501 -
protein localization to kinetochore [Evidence IMP] [PMID
19948764]; GO_process: GO:0006364 - rRNA processing
[Evidence IMP] [PMID 12837249]; GO_process: GO:0006109 -
regulation of carbohydrate metabolic process [Evidence
IGI,IPI] [PMID 12167649]; GO_process: GO:0051726 -
regulation of cell cycle [Evidence IMP] [PMID 10072380];
GO_process: GO:0051726 - regulation of cell cycle
[Evidence IMP] [PMID 7891699]; GO_process: GO:0051726 -
regulation of cell cycle [Evidence IMP] [PMID 8164671];
GO_process: GO:0051726 - regulation of cell cycle
[Evidence IMP] [PMID 9584086]; GO_process: GO:0000903 -
regulation of cell shape during vegetative growth phase
[Evidence IGI] [PMID 12477789]; GO_process: GO:0007346 -
regulation of mitotic cell cycle [Evidence IMP] [PMID
23418575]; GO_process: GO:0031297 - replication fork
processing [Evidence IMP] [PMID 19884341]; GO_process:
GO:0009408 - response to heat [Evidence IMP,IPI] [PMID
10207049]; GO_process: GO:0030847 - termination of RNA
polymerase II transcription, exosome-dependent [Evidence
IPI] [PMID 12819204]; GO_process: GO:0030846 - termination
of RNA polymerase II transcription, poly(A)-coupled
[Evidence IPI] [PMID 12819204]; GO_process: GO:0061587 -
transfer RNA gene-mediated silencing [Evidence IMP] [PMID
23707796]"
/codon_start=1
/product="type 1 serine/threonine-protein phosphatase
catalytic subunit GLC7"
/protein_id="XP_018736862.1"
/db_xref="GeneID:30033833"
/translation="MHGGLSPDLNSMEQIRRVMRPTDIPDIGLLCDLLWSDPDKDITG
WSENDRGVSFTFGPDVVSRFLSKHDMDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYC
GEFDNAGAMMGVDESLLCSFQILKPAEKKKKYGYTSVGTSRPVTPPRKQKRPTK"
gene complement(<44647..>45621)
/gene="GLC7"
/locus_tag="AWJ20_1974"
/db_xref="GeneID:30033834"
mRNA complement(<44647..>45621)
/gene="GLC7"
/locus_tag="AWJ20_1974"
/product="type 1 serine/threonine-protein phosphatase
catalytic subunit GLC7"
/transcript_id="XM_018878894.1"
/db_xref="GeneID:30033834"
CDS complement(44647..45621)
/gene="GLC7"
/locus_tag="AWJ20_1974"
/inference="similar to AA sequence:KEGG_Orthology:K06269"
/note="Type 1 serine/threonine protein phosphatase
catalytic subunit; cleavage and polyadenylation factor
(CPF) component; involved in various processes including
glycogen metabolism, sporulation, mitosis; accumulates at
mating projections by interaction with Afr1p; interacts
with many regulatory subunits; involved in regulation of
the nucleocytoplasmic shuttling of Hxk2p; import into
nucleus is inhibited during spindle assembly checkpoint
arrest; GO_component: GO:0005935 - cellular bud neck
[Evidence IDA] [PMID 11412094]; GO_component: GO:0000778 -
condensed nuclear chromosome kinetochore [Evidence IDA]
[PMID 19948764]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005847 - mRNA
cleavage and polyadenylation specificity factor complex
[Evidence IDA] [PMID 12819204]; GO_component: GO:0001400 -
mating projection base [Evidence IDA] [PMID 10747092];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
11412094]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 23418575]; GO_component: GO:0000164 - protein
phosphatase type 1 complex [Evidence IDA] [PMID 8289829];
GO_component: GO:0005816 - spindle pole body [Evidence
IDA] [PMID 10747092]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA,IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0004721
- phosphoprotein phosphatase activity [Evidence IEA,IEA];
GO_function: GO:0004722 - protein serine/threonine
phosphatase activity [Evidence IDA] [PMID 1660885];
GO_process: GO:0000077 - DNA damage checkpoint [Evidence
IMP] [PMID 19884341]; GO_process: GO:0000076 - DNA
replication checkpoint [Evidence IGI,IMP] [PMID 19884341];
GO_process: GO:0030437 - ascospore formation [Evidence
IMP] [PMID 9584086]; GO_process: GO:0005975 - carbohydrate
metabolic process [Evidence IEA]; GO_process: GO:0007114 -
cell budding [Evidence IMP] [PMID 10639337]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0051301 - cell division [Evidence IEA]; GO_process:
GO:0006873 - cellular ion homeostasis [Evidence IMP] [PMID
11973298]; GO_process: GO:0007059 - chromosome segregation
[Evidence IMP] [PMID 10072383]; GO_process: GO:0070940 -
dephosphorylation of RNA polymerase II C-terminal domain
[Evidence IDA] [PMID 24413056]; GO_process: GO:0005977 -
glycogen metabolic process [Evidence IEA]; GO_process:
GO:0005977 - glycogen metabolic process [Evidence IMP]
[PMID 1660885]; GO_process: GO:0016576 - histone
dephosphorylation [Evidence IMP] [PMID 10975519];
GO_process: GO:0016576 - histone dephosphorylation
[Evidence IDA,IMP] [PMID 19884341]; GO_process: GO:0006397
- mRNA processing [Evidence IEA]; GO_process: GO:0007126 -
meiotic nuclear division [Evidence IPI] [PMID 8754819];
GO_process: GO:0007067 - mitotic nuclear division
[Evidence IEA]; GO_process: GO:0007094 - mitotic spindle
assembly checkpoint [Evidence IMP] [PMID 10072380];
GO_process: GO:0035307 - positive regulation of protein
dephosphorylation [Evidence IMP] [PMID 23418575];
GO_process: GO:0006470 - protein dephosphorylation
[Evidence IDA] [PMID 1660885]; GO_process: GO:0034501 -
protein localization to kinetochore [Evidence IMP] [PMID
19948764]; GO_process: GO:0006364 - rRNA processing
[Evidence IMP] [PMID 12837249]; GO_process: GO:0006109 -
regulation of carbohydrate metabolic process [Evidence
IGI,IPI] [PMID 12167649]; GO_process: GO:0051726 -
regulation of cell cycle [Evidence IMP] [PMID 10072380];
GO_process: GO:0051726 - regulation of cell cycle
[Evidence IMP] [PMID 7891699]; GO_process: GO:0051726 -
regulation of cell cycle [Evidence IMP] [PMID 8164671];
GO_process: GO:0051726 - regulation of cell cycle
[Evidence IMP] [PMID 9584086]; GO_process: GO:0000903 -
regulation of cell shape during vegetative growth phase
[Evidence IGI] [PMID 12477789]; GO_process: GO:0007346 -
regulation of mitotic cell cycle [Evidence IMP] [PMID
23418575]; GO_process: GO:0031297 - replication fork
processing [Evidence IMP] [PMID 19884341]; GO_process:
GO:0009408 - response to heat [Evidence IMP,IPI] [PMID
10207049]; GO_process: GO:0030847 - termination of RNA
polymerase II transcription, exosome-dependent [Evidence
IPI] [PMID 12819204]; GO_process: GO:0030846 - termination
of RNA polymerase II transcription, poly(A)-coupled
[Evidence IPI] [PMID 12819204]; GO_process: GO:0061587 -
transfer RNA gene-mediated silencing [Evidence IMP] [PMID
23707796]"
/codon_start=1
/product="type 1 serine/threonine-protein phosphatase
catalytic subunit GLC7"
/protein_id="XP_018736863.1"
/db_xref="GeneID:30033834"
/translation="MVEPIEEFDVDTIIDKLLTVRTSRPGRMVNLLSTEIDYLCKKSR
EIFIQQSILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSI
ETICLLFAYKIKYPENFFILRGNHEAASTNRIYGFYDECKRRYNIRTWKTFTDVFNCL
PVAAVIDEKIFAMHGGLSPDIHSMDQIRRIMRPTDIPDDGLLCDLLWADPDKDVTGWG
ENDRGVSYTFGVDVVKKFLEKHDLELICRAHQVVEDGYEFFAKRQLVTIFSAPNYCGE
FDNAGAMMSLDENLTCSFQILRPAEKGLSRGKGPFEGRRKKKSRDSDW"
gene complement(<46504..>48150)
/gene="VHR1"
/locus_tag="AWJ20_1975"
/db_xref="GeneID:30033835"
mRNA complement(<46504..>48150)
/gene="VHR1"
/locus_tag="AWJ20_1975"
/product="Vhr1p"
/transcript_id="XM_018878895.1"
/db_xref="GeneID:30033835"
CDS complement(46504..48150)
/gene="VHR1"
/locus_tag="AWJ20_1975"
/note="Transcriptional activator; required for the vitamin
H-responsive element (VHRE) mediated induction of VHT1
(Vitamin H transporter) and BIO5 (biotin biosynthesis
intermediate transporter) in response to low biotin
concentrations; VHR1 has a paralog, VHR2, that arose from
the whole genome duplication; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
16533810]; GO_function: GO:0003677 - DNA binding [Evidence
IEA]; GO_function: GO:0000977 - RNA polymerase II
regulatory region sequence-specific DNA binding [Evidence
IDA] [PMID 16533810]; GO_function: GO:0001133 -
sequence-specific transcription regulatory region DNA
binding RNA polymerase II transcription factor recruiting
transcription factor activity [Evidence IDA,IMP] [PMID
21278159]; GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 16533810]; GO_process: GO:0061420 - regulation
of transcription from RNA polymerase II promoter in
response to biotin starvation [Evidence IMP] [PMID
16533810]; GO_process: GO:0061420 - regulation of
transcription from RNA polymerase II promoter in response
to biotin starvation [Evidence IMP] [PMID 21278159];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Vhr1p"
/protein_id="XP_018736864.1"
/db_xref="GeneID:30033835"
/translation="MNTPDSHQKVEHSLKEPDGNASDNDHMVQLGVGITQAIRSRLNF
TDDYKWKRFSARRLELIDELELSSRKASEQDEKISLVANRLREEYGFPETTLNDFHRL
VRAGIQSVRRNRKRSYLKGTTGPSNPTGNTSSTASGTITPNTSTPTSGHSPILSSDHI
HMPQTKQSYTKLTAELASSNSIQDKRVGLQFENISPDRFKFSNQADSDDESSILPLKR
PRAVRPSPQNNDRISITSLISPSPPVPTHPLTSDQTVLPPVSSLSLASPGPREDLFIL
ALERLQQFTIKLSSHMPVNSNTEFLGQSVMSTAAAYAVERRLPNHESSETVRSYVLSF
TVTSAIVRALGLHSFSLLKITLASCCREYGFDTIIHSLSSFYYEMFNIDPTFDLTKES
SYDKMASILATVTSSRAIPTPVLPVNTGLLSAPGSTITSLTSLSGTAYKSQAPVVRPV
TLRFGNQKLEFTYSPARVSSPPTVSEVLDNGRNAFSINKEQPLQIENRNRPGKILLTD
ADVADTFTEARVDIELRVVQRDRPLASSAAADRPKFQELL"
gene <49106..>50077
/gene="ENV9"
/locus_tag="AWJ20_1976"
/db_xref="GeneID:30033836"
mRNA <49106..>50077
/gene="ENV9"
/locus_tag="AWJ20_1976"
/product="Env9p"
/transcript_id="XM_018878896.1"
/db_xref="GeneID:30033836"
CDS 49106..50077
/gene="ENV9"
/locus_tag="AWJ20_1976"
/note="Protein proposed to be involved in vacuolar
functions; mutant shows defect in CPY processing and
defects in vacuolar morphology; has similarity to
oxidoreductases, found in lipid particles; required for
replication of Brome mosaic virus in S. cerevisiae, a
model system for studying replication of positive-strand
RNA viruses in their natural hosts; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0005811 - lipid particle [Evidence
IEA,IEA]; GO_component: GO:0005811 - lipid particle
[Evidence IDA] [PMID 14562095]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence ISS] [PMID
8972580]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]; GO_process:
GO:0006624 - vacuolar protein processing [Evidence IMP]
[PMID 21912603]; GO_process: GO:0007033 - vacuole
organization [Evidence IMP] [PMID 21912603]"
/codon_start=1
/product="Env9p"
/protein_id="XP_018736865.1"
/db_xref="GeneID:30033836"
/translation="MTFNHETTSEQIAEAFGSQIKGKVVLVTGATWGGIGADNARVIA
ASGAKLVIITGRSQAKLDETIENIQKQTPNAIVRGLILDLSSFKSVRKAAEEVNAYAE
DIDVLINNAGVMACPYSKTEDGFEMQFGTNHLGHFLFTNLILKKLLATPHPRVVNVSS
LGHGLAPIIFGDIGFSDGAKYDKNHAYGQSKTANILFTRELHRRYNKEHGLVAFSIHP
GAILDTNLARHMDINNDYANIEVPKDYWGTGDFAQNMSTLKMKNVSQGGATTLAAAFN
PEIASQSGAYLDDCEIHEEHCRNHAKQSNDAFKLWTVSEELVGQKFD"
gene <51489..>53402
/gene="DSF2"
/locus_tag="AWJ20_1977"
/db_xref="GeneID:30033837"
mRNA <51489..>53402
/gene="DSF2"
/locus_tag="AWJ20_1977"
/product="Dsf2p"
/transcript_id="XM_018878897.1"
/db_xref="GeneID:30033837"
CDS 51489..53402
/gene="DSF2"
/locus_tag="AWJ20_1977"
/note="Deletion suppressor of mpt5 mutation; relocalizes
from bud neck to cytoplasm upon DNA replication stress;
GO_component: GO:0005934 - cellular bud tip [Evidence IDA]
[PMID 22842922]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008150 -
biological_process [Evidence ND]"
/codon_start=1
/product="Dsf2p"
/protein_id="XP_018736866.1"
/db_xref="GeneID:30033837"
/translation="MSLQDQQISGNTFTPNLSPLDLLEQQSRQLAHDLHSSKPTDRHK
RNSADTIKGVSEPNNPAPERGHVVNRSIDTHSSRLSQNGDTMTEQVPDQVSQQQSPSR
VRHKHMASDTSTISTASSYQPNSHSQYATPDSIASNNIKSYNAPLRPIQNFNQDTNQE
WTPRSRASMSSLSSFGGGGGILDSFRLSALPSFDRQSVIDTEPSSDNLSDISRAGSLQ
TIRSVSGNVRTSFDPRMHIRTVDSGRDANPKNDIEDSESTSPDFGKTHAETNGEASSE
RPQLVSNKTFSHNIPSRPVILSAKSYHQSASTSMSPDSTVSTPPSSKSRPVSDASSYY
SSTSRFSQSSIKAPAALFQQFSHPTTTNVAHKSRPTGYFFPEPTSRPAVGKHQPQPAK
AGNGGLLIPPSIRKSPPPIQTRKTSNDSTNSRNLIPQSLLEKPFADMTVEDHVTIGIA
YHESGDLREASYHWQHAAFKGDHTAMLLYGLSLRHGWGMRQNPTEAVQWLRKAMESTI
AEGMFKDGDFHKSNVSKEGSEDSGKVKKAHIGLALYELGMSYLHSWGIEKDETMALKS
FELAGQFGDADALCEAAALYMHNGPKGRKKDLMKAAKLYRKAGELGANMVGQSWVYKD
KYMEDSKKDKKKK"
gene complement(<53723..>53989)
/gene="MZM1"
/locus_tag="AWJ20_1978"
/db_xref="GeneID:30033838"
mRNA complement(<53723..>53989)
/gene="MZM1"
/locus_tag="AWJ20_1978"
/product="Mzm1p"
/transcript_id="XM_018878898.1"
/db_xref="GeneID:30033838"
CDS complement(53723..53989)
/gene="MZM1"
/locus_tag="AWJ20_1978"
/inference="similar to AA sequence:KEGG_Orthology:K18170"
/note="Protein required for assembly of the cytochrome
bc(1) complex; acts as a chaperone for Rip1p and
facilitates its insertion into the complex at a late stage
of assembly; localized to the mitochondrial matrix; null
mutant exhibits a respiratory growth defect and reduced
mitochondrial zinc levels, which is characteristic of
mutations affecting bc(1) complex assembly; member of the
LYR protein family; human LYRM7 is a functional ortholog;
GO_component: GO:0005759 - mitochondrial matrix [Evidence
IEA]; GO_component: GO:0005759 - mitochondrial matrix
[Evidence IDA] [PMID 20404342]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14562095];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 17054397]; GO_function:
GO:0044183 - protein binding involved in protein folding
[Evidence IMP,IPI] [PMID 22927643]; GO_process: GO:0034551
- mitochondrial respiratory chain complex III assembly
[Evidence IMP] [PMID 21807901]"
/codon_start=1
/product="Mzm1p"
/protein_id="XP_018736867.1"
/db_xref="GeneID:30033838"
/translation="MSTTNRTAALSAYRYVLRATRVAFNSDPVALNGSRSQIRAGFKA
DRNVTDEAEIKEKIQYIKDIGLILRTNVVQAQKKSEDDNNFGEF"
gene <54568..>56172
/locus_tag="AWJ20_1979"
/db_xref="GeneID:30033839"
mRNA <54568..>56172
/locus_tag="AWJ20_1979"
/product="hypothetical protein"
/transcript_id="XM_018878899.1"
/db_xref="GeneID:30033839"
CDS 54568..56172
/locus_tag="AWJ20_1979"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736868.1"
/db_xref="GeneID:30033839"
/translation="MPPRRKRGAATKEVTNGDSSPRTTRASAAKKAKETTTDSKPKRS
SSPQNSELASTVEPKVKSKGKNTKQPVVEPSEEEDEAISHTPNQEEPEENKENENVGI
KSKLKKVQSPPRNGLLTSALGISLENTPVTRSNKRQHDGEDTDDSPSIVSRSKKHKNS
ILSNGEANTSLSTLSQPPKKAVKKPWRTGTATAANSAETASKEGGPTGTIDEDTSSKS
KPNTTEKPLLKDKSSNPHIISKVTNGISATTVKSSTVTKTDKVALSNPRLISNSSVTG
LISPSKPAHPGNLASSISSHGRLSPTKSHPRVAVGLSPSKHGASDSTLSSALTSFTVN
AASELAVKFEKLRDLRETKAEELLNEFKLAAEARFQASDELIRELRLHEEKNQHSQDQ
ELVDANIALQNLQQNVANLNSDLEAANEEISLLKAKLEAKENQQSQNMSNLALGICSD
LSGLVILKVLNEEDEYTYECVQSGRNGVFRYNLSVAKSDSKEESHDITFTPLLTEKDE
HLTSLLPDYFTEPLSFTREAVSILWC"
gene complement(<57205..>59694)
/gene="MEF2"
/locus_tag="AWJ20_1980"
/db_xref="GeneID:30033841"
mRNA complement(<57205..>59694)
/gene="MEF2"
/locus_tag="AWJ20_1980"
/product="Mef2p"
/transcript_id="XM_018878901.1"
/db_xref="GeneID:30033841"
CDS complement(57205..59694)
/gene="MEF2"
/locus_tag="AWJ20_1980"
/inference="similar to AA sequence:KEGG_Orthology:K02355"
/note="Mitochondrial elongation factor involved in
translational elongation; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0005525 - GTP binding
[Evidence IEA,IEA]; GO_function: GO:0003924 - GTPase
activity [Evidence IEA]; GO_function: GO:0003924 - GTPase
activity [Evidence ISS] [PMID 19716793]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0006184 - GTP catabolic process [Evidence
IEA]; GO_process: GO:0000002 - mitochondrial genome
maintenance [Evidence IMP] [PMID 21414316]; GO_process:
GO:0032543 - mitochondrial translation [Evidence ISS]
[PMID 19716793]; GO_process: GO:0051881 - regulation of
mitochondrial membrane potential [Evidence IMP] [PMID
21414316]; GO_process: GO:0032790 - ribosome disassembly
[Evidence ISS] [PMID 19716793]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="Mef2p"
/protein_id="XP_018736869.1"
/db_xref="GeneID:30033841"
/translation="MGVGVRLNCFSILPKFVLAKSSIYKYATAVPHGTRFYSVSRPCF
SESSAVRNIGIIAHIDAGKTTTTERMLYYSGLTSHIGDVDQGDTVTDYLQAERERGIT
ITSAAVTLDWSGHKINLIDTPGHADFTFEVIRSIRVLDGAVTVLDGVAGVEAQTEKVW
RQASDRGIPKIVFINKMDRPGAGFGRTVREVVAKLNTRVGITNFPYFGQMDAGHEGRF
QGVVDVVDKRLILWDEGSDGKSVKVSELEDGSELSQECERIRTSLIETLCECEGNDDL
VEEVLEISDYMAVPPSSIRKALRNSTLREDISPVLCGASFRNIGVQPLMEAVVNYLPS
PEERKAPDAISKVPVSKGKHAAQSIKSENVTLNVNDPKLTCALAFKVVNDPIRGILVY
VRVYSGTLQTGSVILNTTNGKKERVSKLLQMQADMPLDISKIEKGNIGVIVGSKEIRT
GDTLVAHGLKKDGITTLLKRDLGLHLHPIDVPPPVFFASITPKSISDTRNMEEALDIL
LREDPSLRLSYDDDSGQTLLSGMGELHLEIARDRLINDLKAKVKIGPIMISYKESIQA
SSKIIEKSYSEGGNEDMSVKVGLMVEPVYEDIELGDLAEKIDPHDNHYVIFPSYTNHP
HLSQEEIYEAIKIGIIPALATGGKLARLPLHSISVKITNLEIPEDCTSTASISSAVRL
ASQEALRGFIKSDYVIMEPKMDVRVVVNEEDIGNVVNDISSTRRGHVISLNEDDAENS
SDQTSISNHFKELAASVYVPPDYTMYMSKHGDRATSQQSLIKAKIPLRKMVGYLTSLR
SMTQGRATFLMSFDKYEIAPPDAIDEIVDSL"
gene <60189..>61094
/locus_tag="AWJ20_1981"
/db_xref="GeneID:30033842"
mRNA <60189..>61094
/locus_tag="AWJ20_1981"
/product="pre-mRNA splicing factor SLU7"
/transcript_id="XM_018878902.1"
/db_xref="GeneID:30033842"
CDS 60189..61094
/locus_tag="AWJ20_1981"
/inference="similar to AA sequence:KEGG_Orthology:K12819"
/codon_start=1
/product="pre-mRNA splicing factor SLU7"
/protein_id="XP_018736870.1"
/db_xref="GeneID:30033842"
/translation="MERPRKIGAKFSGKDIVADDVLQDITTTWDSKRDRWNGYDAHEY
KKVVERFEEKERQKKADGELEDDDDDELKKTQDGQLGEKVRSLRERGDKAAYLEDLSE
DAVEFNPKTRTMRTEGGTINDNGQFERKLSDSALEYENLRQKAEGLSEQGMAIHMEAS
PTEAVLKLKQLQQEKESQKHKLKQELLEKYGGAEHLAPRPKEVEIVPLEASTVTKSGE
DKPGLKPSSIKSRYPEDIYPGNHSSVWGSYWINGKWGFACCHSLVKHSYCLGEKGIEI
NDSKEEVPQVSTEHPNKRSYDEVSQ"
gene <63464..>65449
/locus_tag="AWJ20_1982"
/db_xref="GeneID:30033843"
mRNA <63464..>65449
/locus_tag="AWJ20_1982"
/product="hypothetical protein"
/transcript_id="XM_018878903.1"
/db_xref="GeneID:30033843"
CDS 63464..65449
/locus_tag="AWJ20_1982"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736871.1"
/db_xref="GeneID:30033843"
/translation="MAPFWKKLNSPQSFANHDFTSNFLELALLYDVLRNAKFTTVRKR
MELPHDSLCKYDSDMVSAVTQSSIFLVHSDNVMEVKVALLKELMLVSSDHGTHNSLAG
SASTSANTTELDTTEVVYLDTPKLEYAQTGKEPAQIRKVNDSSSVLCSPTGGLRHFAS
SNLTPEELDLILNEKAAEIDFNQLDGARKLAIGWVQRKQAKPLSKVTYSRNRFSYVND
DDAASVIGVNVNNSTETNIWATMDSNIKLEKTTKTELSSAKTFPHSILEIRWKGTSKP
QWLSDIEKSHLVYPMREGFSLYAYSVVSFYSDQLQSVPSWLEIIEDNVEIRKVPSVAP
VRRSMNSSANLAGANRTDKSVSISTPSILLNDSNAQIHDYDSAKEQEGLSRKSSMTVV
EDRENRPQMLSRVSSFYNPASRQRHKLQSQPSESKLAVRYWNEFDDPEDGEDLGVFVI
NDEDPSGSYQDKLFSEANIDALVRISDKFLQSIGWFTSKITGSRDGYEALREGNYRRG
SSSESDPDEEDEEEDDFDEEASVGRYSPRIKPRTSSDDSDDFAYRSFGDRNNSVAAQR
RNSVLTFLYSMCLSLAAFIIMTLFSVTLLQDISEISLGTYVFMISGFIFALAISMLGL
SLYLLCEAPPWWQQTIVFSSFFSIVCFGVGGIAWILT"
gene complement(<65534..>66802)
/gene="ARP6"
/locus_tag="AWJ20_1983"
/db_xref="GeneID:30033844"
mRNA complement(<65534..>66802)
/gene="ARP6"
/locus_tag="AWJ20_1983"
/product="Arp6p"
/transcript_id="XM_018878904.1"
/db_xref="GeneID:30033844"
CDS complement(65534..66802)
/gene="ARP6"
/locus_tag="AWJ20_1983"
/inference="similar to AA sequence:KEGG_Orthology:K11662"
/note="Actin-related protein that binds nucleosomes; a
component of the SWR1 complex, which exchanges histone
variant H2AZ (Htz1p) for chromatin-bound histone H2A;
GO_component: GO:0000812 - Swr1 complex [Evidence IDA]
[PMID 14645854]; GO_component: GO:0000812 - Swr1 complex
[Evidence IDA] [PMID 14690608]; GO_component: GO:0000812 -
Swr1 complex [Evidence IDA] [PMID 16299513]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11011149];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0031491 - nucleosome binding [Evidence
IMP] [PMID 16299513]; GO_process: GO:0016568 - chromatin
modification [Evidence IEA]; GO_process: GO:0006338 -
chromatin remodeling [Evidence IDA] [PMID 14645854];
GO_process: GO:0006338 - chromatin remodeling [Evidence
IGI,IMP] [PMID 14690608]; GO_process: GO:0043486 - histone
exchange [Evidence IMP] [PMID 16299513]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Arp6p"
/protein_id="XP_018736872.1"
/db_xref="GeneID:30033844"
/translation="MSVLVIDNGAYELKIGYATDDKNPAHFRVSNSITRSKDRRTYVG
NEIENCRDYAGLTYRRPHERGQLVNWETQKAIWDNQFYGKTSKLVGMNGGKPVDPSET
SLILTEMPYSLPALSSNMDQIVFEEYGFDSYYRCTPGSLVPWNDIRELYNDTSSRSSP
VSECALVIDSGFSSTHVLPVVLGEVYWPAAKRINIGGKHLTNYLKETVSFRYYNMMEE
TFLMNVIKERVCFVSLKYQLDLEEAHINKRKSLLRIDYALPDYRTSKLGHIVKGATSS
NDSNQVLTLTNERFTIPEALFRPSDLNLDQAGIPETAINCVKLMPEEIQSMLLSNVVL
VGGNAVLPGYKERIREELQSFSPQGVNLRVGMPVDPISYAWQGGCRLGSQKDMLSKAY
VTKADYMEHGVNLCLSRFGIKKMDNSIAMD"
gene <67251..>68048
/locus_tag="AWJ20_1984"
/db_xref="GeneID:30033845"
mRNA <67251..>68048
/locus_tag="AWJ20_1984"
/product="hypothetical protein"
/transcript_id="XM_018878905.1"
/db_xref="GeneID:30033845"
CDS 67251..68048
/locus_tag="AWJ20_1984"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736873.1"
/db_xref="GeneID:30033845"
/translation="MPRTRSLGQAGTAVPRRPTRVVEDDSDEDDSNPGMYSKLSTDTM
VPLVSSTPMRPGAVQVAHLEPVSRLSTIEDVDSSKENEFSPTDALGKMPPVTPARKQN
RVSFAPIPSGKPTNGSAPPTLAPSHQALARLGNAGHPLTELDLNNRLPPPPQTPLAYP
ATPSEDLDSTIEAVRKVSLDSTSPTFRSPSKIANYLEQSSETPHQRLTIHKLVLTNFK
SYAGRQEIGPFHSVSTMKKSTNHSCTNFTTVFFCSSGPQRIGKIQRY"
gene <68086..>71694
/gene="SMC4"
/locus_tag="AWJ20_1985"
/db_xref="GeneID:30033846"
mRNA <68086..>71694
/gene="SMC4"
/locus_tag="AWJ20_1985"
/product="condensin subunit SMC4"
/transcript_id="XM_018878906.1"
/db_xref="GeneID:30033846"
CDS 68086..71694
/gene="SMC4"
/locus_tag="AWJ20_1985"
/inference="similar to AA sequence:KEGG_Orthology:K06675"
/note="Subunit of the condensin complex; condensin
reorganizes chromosomes during both mitosis and meiosis;
forms a subcomplex with Smc2p that has ATP-hydrolyzing and
DNA-binding activity, but other condensin subunits are
required for chromatin binding; required for tRNA gene
clustering at the nucleolus; potential Cdc28p substrate;
GO_component: GO:0005694 - chromosome [Evidence
IEA,IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0000799 - nuclear
condensin complex [Evidence IDA] [PMID 10811823];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22842922]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0016887 - ATPase activity
[Evidence IDA] [PMID 12719426]; GO_function: GO:0003682 -
chromatin binding [Evidence IDA] [PMID 11864994];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_process: GO:0006310 - DNA recombination [Evidence
IEA]; GO_process: GO:0006281 - DNA repair [Evidence IEA];
GO_process: GO:0006268 - DNA unwinding involved in DNA
replication [Evidence IMP] [PMID 24062159]; GO_process:
GO:0007049 - cell cycle [Evidence IEA]; GO_process:
GO:0051301 - cell division [Evidence IEA]; GO_process:
GO:0030261 - chromosome condensation [Evidence IEA];
GO_process: GO:0051276 - chromosome organization [Evidence
IEA]; GO_process: GO:0010032 - meiotic chromosome
condensation [Evidence IC] [PMID 10811823]; GO_process:
GO:0051307 - meiotic chromosome separation [Evidence IC]
[PMID 10811823]; GO_process: GO:0007076 - mitotic
chromosome condensation [Evidence IMP] [PMID 10811823];
GO_process: GO:0007067 - mitotic nuclear division
[Evidence IEA]; GO_process: GO:0000070 - mitotic sister
chromatid segregation [Evidence IMP] [PMID 10811823];
GO_process: GO:0070550 - rDNA condensation [Evidence IMP]
[PMID 10811823]; GO_process: GO:0007130 - synaptonemal
complex assembly [Evidence IC] [PMID 10811823];
GO_process: GO:0070058 - tRNA gene clustering [Evidence
IMP] [PMID 18708579]"
/codon_start=1
/product="condensin subunit SMC4"
/protein_id="XP_018736874.1"
/db_xref="GeneID:30033846"
/translation="MRHSKVSALIHNSAAYPNLNSCSVEVHFQDVVDSANGSSTVVPG
TELVITRKSFRNNSSKYMINDKESNFTEVTTLLRERGIDLDHKRFLILQGEVESIAQM
KPKADNDNDDGLLEYLEDIIGTSQYKKAIEESLAEVEVLNEDCVQKQDRLKIVETELN
SLEDQKNDIVTYLELENELAFKQSALWQLQAYLCQKRVELSKSIVEEQHAKLKAELDR
TTVNSEEIEVLKKDHAQKLKELDLIKKTLSEKSKLLSKHEIEKVKVEERKKHLDSKRK
KLEKSIEAATHSKVETETWLSNYQEESSQLSDQLKGLQKSLENENCQLEAIQDELKDK
TAEFTDQIETIQKKLEPWREKTRSKESEIAVANSELELLKERESQGEKSLNESKENIT
ITMKEGRAKEREMEKLKKELEHVTSQISMGVKECDQASIKLAKMKQQLVNGRQRVDAA
KEALSSTTSQNKVLTGLTRLNDSGRIQGFRGRLGALGTIDAKYDVAISTACPSLDNMV
VDTVAAGEQCVDYLRKQNLGRAKFILLEKLPKRDLSPIQTPENVPRLFDLIVPSAPEY
APAFYSVLFDTLVAKDADQARRIAYGKKRWRVVTLDGVIVEASGAMSGGGEISRGKMA
AGLTEGALSEGQVQELERVQDEHEKKYKIAENTVLTMQTTLKELQERKPEIELEISKV
ELDITALTARLNEAKKQSAELAKSLEANRPDPSTLKRSEEHVAKLEAELKQLRSHSEG
LEQDVADLQEKIMDAGGVRLRVQKSKVDGIKEQIELVNTRLANGLKENAKANNDFKKH
SKTIDNCEAEISASTQELEEANKELSEKQLIIQQFEQLVSQLSDQLEEKQEILDELKS
ELDAKQKDIDSLRSREIEIKNTIDQHEKTIKDESNSLRSWISQLQKLQLHDLSEIKAV
RVKKEKPLKKSLPRQDVEEGEEAEGNEEDEDVNEDEDVANKSILNKTELFEYSADELE
AMNKAELKREIESLQTKGQNAKVDMQILKDYKRRVEEHDARRLALNESVTARDTVKKM
CDDLRNRRLEEFMTGFNSISGKLKEMYQMITMGGNAELELVDSLDPFSEGILFSVMPP
KKSWRNISNLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFRNVSIVATYIKE
RTKHGQFIVISLRNNMFELAKQLVGIYKVNNMTKSIALQNNDYIHGQDSSNTTILP"
gene complement(<71831..>72745)
/gene="COQ5"
/locus_tag="AWJ20_1986"
/db_xref="GeneID:30033847"
mRNA complement(<71831..>72745)
/gene="COQ5"
/locus_tag="AWJ20_1986"
/product="2-hexaprenyl-6-methoxy-1,4-benzoquinone
methyltransferase"
/transcript_id="XM_018878907.1"
/db_xref="GeneID:30033847"
CDS complement(71831..72745)
/gene="COQ5"
/locus_tag="AWJ20_1986"
/inference="similar to AA sequence:KEGG_Orthology:K06127"
/note="2-hexaprenyl-6-methoxy-1,4-benzoquinone
methyltransferase; involved in ubiquinone (Coenzyme Q)
biosynthesis; localizes to the matrix face of the
mitochondrial inner membrane in a large complex with other
ubiquinone biosynthetic enzymes; GO_component: GO:0005759
- mitochondrial matrix [Evidence IDA] [PMID 9083048];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0043334 - 2-hexaprenyl-6-methoxy-1,4-benzoquinone
methyltransferase activity [Evidence IMP,ISS] [PMID
9083049]; GO_function: GO:0008168 - methyltransferase
activity [Evidence IEA,IEA]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0009060 - aerobic respiration [Evidence IMP] [PMID
9083049]; GO_process: GO:0032259 - methylation [Evidence
IEA]; GO_process: GO:0006744 - ubiquinone biosynthetic
process [Evidence IEA,IEA]; GO_process: GO:0006744 -
ubiquinone biosynthetic process [Evidence IMP] [PMID
9083048]"
/codon_start=1
/product="2-hexaprenyl-6-methoxy-1,4-benzoquinone
methyltransferase"
/protein_id="XP_018736875.1"
/db_xref="GeneID:30033847"
/translation="MIVKRALARAQKQSNSLRFLNCCNTAITGEPLGFVRRPYSTEGS
KTTHFGFKTVPEDEKEHLVKHVFSNVASSYDYMNDAMSLGIHRLWKDHFVSKLDPGRR
AGGSPLSFLDVAGGSGDIAFRILDHAAKIHGDHESVMNVVDINPEMLAEGEKRSKETP
YGNTSRINFKVQNAEFLDAIPDNSQDIYTVSFGIRNFTNIPAALATAHRVLKPGGVFA
CLEFSKVDNSILDTLYQQYSFNIIPLLGQVLVGDRDSYQYLVESIQRFPSQEQFAGMI
QDAGFTLAGSGYENLSFGIAAIHTGVKL"
gene <73476..>73982
/locus_tag="AWJ20_1987"
/db_xref="GeneID:30033848"
mRNA <73476..>73982
/locus_tag="AWJ20_1987"
/product="hypothetical protein"
/transcript_id="XM_018878908.1"
/db_xref="GeneID:30033848"
CDS 73476..73982
/locus_tag="AWJ20_1987"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736876.1"
/db_xref="GeneID:30033848"
/translation="MLRHYNHLLRAVKVAPSVQVRYTQPLLFGGIAGKLSPWNSTCRG
FSSTNSRSMIPVAPIPFDDPLGGDEEDPKKKKDSKKETGRGYDIFMKCLETAGIMVAS
LSMLGLAGLIYHRTYKERALTKMSNAFTEGNPAFELSMHQNGEDDRNDWLVPASTYSY
NMIQLTAS"
gene <74203..>75435
/locus_tag="AWJ20_1988"
/db_xref="GeneID:30033849"
mRNA <74203..>75435
/locus_tag="AWJ20_1988"
/product="hypothetical protein"
/transcript_id="XM_018878909.1"
/db_xref="GeneID:30033849"
CDS 74203..75435
/locus_tag="AWJ20_1988"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736877.1"
/db_xref="GeneID:30033849"
/translation="MKKTNSLYCTLLDAHADPEIFRIRLGKALNFQFHEDYLGSLFSI
RGPRDTTAILDIERAFNTLETVAVKHVAKHKRPLILIINNAHMIRDDLEGQNLVELLQ
QKAESLSGAGLVTIVFNSDDYWLYERLKKLGTRLEVLTINDFSRKESIQALRRARMKF
YGQKISDEDANKVYNLIGGRPQHLAAVASKDDPINACHKLIDKEKTWFLNQCALLGES
MDDDVMESGKFSTSAMYLMKELVRLDRERLSKMTPEELATSNHEIPRIPLWRARQIMT
RPDYIQHYDNLNIFTLDSLSRVKADSVVMMQAFHEIASLPGFEELLEETADRVSAIES
LGRTRELVAKDLVLDGQYKISHSRNETMIKLEMPEEAENDDDERYKLDRREGFWYKGR
ADKFKKRQEQKENEKGKI"
gene complement(<75545..>77095)
/locus_tag="AWJ20_1989"
/db_xref="GeneID:30033850"
mRNA complement(<75545..>77095)
/locus_tag="AWJ20_1989"
/product="DNA replication protein Dre4"
/transcript_id="XM_018878910.1"
/db_xref="GeneID:30033850"
CDS complement(75545..77095)
/locus_tag="AWJ20_1989"
/codon_start=1
/product="DNA replication protein Dre4"
/protein_id="XP_018736878.1"
/db_xref="GeneID:30033850"
/translation="MSVSKDDTLPTGWTRHLAPSGHFYYYHAESKRSTYERPKSDSQV
IASVKNNNSSESTSRIPTGPRTENQNHERTEKPKQSNQKRKRKKDLRSIDPPQVKVAV
KDCDPWLIVILKSGRWFVYNPQTNKSFWDVPEDVLRKIEEMDRDDLILLVARARGLTL
STDSAAPKFEEQGRLEAGTEEGNANSAPDNTVIEDVSDDEGVESDRENDDDNIDLNDE
NSLEEMSNDDDGLGTGFELDWDNIEDDLKNDDSTGNADLTNQERLSIFYSLLEDFSIN
PFGVWEVELNKVLDDDRYDILESNSARSDAFDKWAALKIREQKLQHEELSTKTQDSTE
DIIKKMVEDSPVGRFLSLVRRKYKEKYFYVDFKRKYRNEALFDIPELTDKDKEKIYRE
YASYKKKPQNDQITLVKEFLSSKIKHSSDLSKLTDTLFNDVRFYIVPDDQISKLVDET
LLKLQTQKTSSSDRSIQDRQNKILAEKAKQQRQLSYERRKLEEEVEKLDEAKYSLKRN
LSTVLGGK"
gene <77713..>78273
/gene="PRE9"
/locus_tag="AWJ20_1990"
/db_xref="GeneID:30033852"
mRNA <77713..>78273
/gene="PRE9"
/locus_tag="AWJ20_1990"
/product="proteasome core particle subunit alpha 3"
/transcript_id="XM_018878912.1"
/db_xref="GeneID:30033852"
CDS 77713..78273
/gene="PRE9"
/locus_tag="AWJ20_1990"
/inference="similar to AA sequence:KEGG_Orthology:K02728"
/note="Alpha 3 subunit of the 20S proteasome; the only
nonessential 20S subunit; may be replaced by the alpha 4
subunit (Pre6p) under stress conditions to create a more
active proteasomal isoform; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0042175 -
nuclear outer membrane-endoplasmic reticulum membrane
network [Evidence IC] [PMID 9087403]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IC] [PMID 9087403];
GO_component: GO:0000502 - proteasome complex [Evidence
IEA]; GO_component: GO:0005839 - proteasome core complex
[Evidence IEA]; GO_component: GO:0019773 - proteasome core
complex, alpha-subunit complex [Evidence IEA];
GO_component: GO:0019773 - proteasome core complex,
alpha-subunit complex [Evidence IDA] [PMID 9087403];
GO_component: GO:0034515 - proteasome storage granule
[Evidence IDA] [PMID 18504300]; GO_function: GO:0004175 -
endopeptidase activity [Evidence IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_function: GO:0008233 - peptidase activity
[Evidence IEA]; GO_function: GO:0004298 - threonine-type
endopeptidase activity [Evidence IEA,IEA]; GO_process:
GO:0010499 - proteasomal ubiquitin-independent protein
catabolic process [Evidence IDA] [PMID 19162040];
GO_process: GO:0080129 - proteasome core complex assembly
[Evidence IMP] [PMID 14739934]; GO_process: GO:0043161 -
proteasome-mediated ubiquitin-dependent protein catabolic
process [Evidence IDA] [PMID 11545745]; GO_process:
GO:0043161 - proteasome-mediated ubiquitin-dependent
protein catabolic process [Evidence IDA] [PMID 19029916];
GO_process: GO:0006508 - proteolysis [Evidence IEA];
GO_process: GO:0051603 - proteolysis involved in cellular
protein catabolic process [Evidence IEA]; GO_process:
GO:0006511 - ubiquitin-dependent protein catabolic process
[Evidence IEA]"
/codon_start=1
/product="proteasome core particle subunit alpha 3"
/protein_id="XP_018736879.1"
/db_xref="GeneID:30033852"
/translation="MYILDDHIVCAVAGIDSDASILVNRARVFAQSYLKTYSEEIPCE
ILVKRLCDIKQGYTQYGGLRPFGVSFVFAGYDDLHGFQLYTSNPSGNYSGWKATSVGA
NGASAQTLLKQEYKEDFSLDQACELAMKVLSKTMDSATLTSEKLEFSTVGKDSASGKV
YQKIWKPADIDALLKATGLGKAEEDD"
gene complement(<78447..>79427)
/locus_tag="AWJ20_1991"
/db_xref="GeneID:30033853"
mRNA complement(<78447..>79427)
/locus_tag="AWJ20_1991"
/product="hypothetical protein"
/transcript_id="XM_018878913.1"
/db_xref="GeneID:30033853"
CDS complement(78447..79427)
/locus_tag="AWJ20_1991"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736880.1"
/db_xref="GeneID:30033853"
/translation="MNLKGSENIQIKGTRAISREVMPKMSPFDATSEADSGRKGLRNS
SSPQLPDELWVAVFEFLDIPDLYNCSKVDHRFRRLAQDPQLHHRRLTQTAKWLERAYP
LRPSCKDLYDRNILLSRVQLAPSPCQKHILASTRLARIFVKDSLRRGLSRRPTRKELI
DRGVMKHGGSIFADKIGSLERQRVVDIIRGFFSDSQRPSLEAAIRRGVVSPPDQENKP
VRVLTRIFSYCFRESSESPRSSHATRMLIDPPTRANVHRMTEWFEELARNERSSFRFG
TQPSQTSSSFGPYAFSTVATTLSSPSPVHQISGISYDGVSAIRQRFLAIS"
gene complement(<80095..>87144)
/gene="TAO3"
/locus_tag="AWJ20_1992"
/db_xref="GeneID:30033854"
mRNA complement(<80095..>87144)
/gene="TAO3"
/locus_tag="AWJ20_1992"
/product="Tao3p"
/transcript_id="XM_018878914.1"
/db_xref="GeneID:30033854"
CDS complement(80095..87144)
/gene="TAO3"
/locus_tag="AWJ20_1992"
/note="Component of the RAM signaling network; is involved
in regulation of Ace2p activity and cellular
morphogenesis, interacts with protein kinase Cbk1p and
also with Kic1p; GO_component: GO:0005933 - cellular bud
[Evidence IDA] [PMID 12972564]; GO_component: GO:0000131 -
incipient cellular bud site [Evidence IDA] [PMID
11854408]; GO_component: GO:0043332 - mating projection
tip [Evidence IDA] [PMID 12972564]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0007118 -
budding cell apical bud growth [Evidence IGI,IMP] [PMID
12972564]; GO_process: GO:0007114 - cell budding [Evidence
IGI,IMP] [PMID 11854408]; GO_process: GO:0000902 - cell
morphogenesis [Evidence IGI,IMP] [PMID 11854408]"
/codon_start=1
/product="Tao3p"
/protein_id="XP_018736881.1"
/db_xref="GeneID:30033854"
/translation="MFWRKSKSEAAATAAQSATNHSSSKNNSTGNLAELKEAALRMER
KSLISIYILCRVLMEVVKQSPPDVLGDDLSEKLEEIVFKQLRTGDPRTLSMSVIRREN
WNLFAELLGEMSNSRFLSVGDRFIAELEKVPSVISKEQEPHILLLINGMKYLKLKIYP
MDALEESAEFIESLGKFFSRSNSTPVKIAYSEALSQILLPLAGVATAELNHPMWVKPV
ETIFAKAMEMIAKPKYWAVAFQLATVILSVAPQELFARHWLTLFESHASKIKDKANRA
VIYIGVSRLLWVFVFRWSETLNSTTKKLDIVSKVLFNPGNKKLWTSWESGITTAACVY
LIRIGSHGYLQYVLDNILLQMVHSGSVDTLTLESIIPERATVGVRSFMYILSDHETTG
TSASRPPFPDDVVLQSVMSNKDRSEPKHIPLPTGSLLQFYEKCCKCIVKLAVLCDTQF
GAHITSVDDRPIQLKPMSVSFHFGNNDSYAQHQRLAYLELFSAVIDFIPWCHFPDTQT
LTRTTEILCRNVVHSNPKVAQASISALKCLVETRDTRAIVNIYTKLMFSFDEKIFTSY
DCPPSSASEFEKILQVYVSLLEIWIANIRVLTKRDANQSETSGSLSVSQPSDDDSSRR
SDDMILNNWWSAVEEIEGNGLFFLCSQDRAIRLLAVKIIRLTTEFDEAIRDHGQHMTK
SHSRTPSKSTLTPPCRVIQLMETVDLTELFEASKPNIELSMPERSRLAKLHGKKKETL
VALAESDYGVDTALWLKAFPTFIKKCFENYPIPIAICRNIVCVRLVRMYDVVVEFSRS
EAVAGTANYAFILKHPMRTHPEVVVEQWRTYLIVACSTLTLTDEQKLHIPDSRYQHGR
KKSAQKITIHHQRITSVRSVFRMVIPLLGVDHPVIRDAIITGIGCINVNIYKALIECL
QPAIETWAEESQRLNQSQSNQSNVSSKQTVGIRKDSSAAISLAYRQERVITEIVHLLY
ITSHYLELPSVAEDEWIISEFVGFLNTLRNFLSLPRVQVDYNFQKLRRYFCGLLENVF
LGVSRNTERSNPISFESRMRYFTLIEDWCTVGQHWNVAREREAKMKRAALSTVKDSRE
HNIILASMELEKKKSDIAILSVLAALCAGPLMQPLDAAGRRKSNVSFNISGLLKWIGT
MFESPNDEMHSLGKRALKNLLIHNPDHPIIFEETVANGYRIHSEPKASKSYFVALAEI
LLEIPDYPCEVRQALALGLFKTGDEDDEVRSLAVDLLKATEIRFYGTSCLQEYRVNIT
NSTPVVYKRAMFNLSSRFAKDHPQETYLVFSELTKFFHVVNDISRRDILAVLLPWIQA
VELQVEVNDMPTPSTAMVMNNLFEITVTFSNRIQNEVEALWVALGNGKYPNNVNAIMN
FVLHHSLERRDPVFVEYSRKILAYLAAPPAGTTLIDALMKYLQPKAMIPQHLEPYDLS
IAERMYPYVATISAVLPVGNKETTFSHGQLALILLVDLIVSPLESVKANLPLILHVSL
VLFDHYLPIINDRSRELLVHVVNEFAGSQAGAQEFCELIRVSNSKFNWSYDNLNSDKN
GARTPKMMVQMIKDILGIFGTQIPDLRENWSRVALMWATTCPVRHIACRSFQVFRCLL
FTLDQNMLADMLARLSNTISDSTTDIQGFAMQILMTLNAVTAELASEDLINYPQLFWA
TVACLGTVHEQEFIEVLSILEKFISKIDLDSAETVACLIATFPPKWEAKFRGLQHAVI
PGLRSSLAWEQSLRILDRLNQLQDSEIIAGPDRLALAISINLPRYLHALEIGETTPEI
EASSDRLRQMCDERGLGALSRILVSFGKGRFRSKQDFLNQTIQALQNNFFPSCEGSVL
IFLLGILSNKIKWVKTETMDILKKILPMVDLQKEEFAGVGADIISPLLRLLQTDYAEQ
ALGVLDETISISASNMDKHVLRMSLGNRTIRKEYEKTATMFGIPDESGWAIPMPAVTA
NITRNNVHAVFYTCNTTPSTEQIPVSAEEFQFHKEEYGFMGGPLDRTDSVSVAEDVEG
GSLSHMWTALDNLDSFFTRDMKKSRQQRPNHEHNISVTDTDMSTDNNLDPVESVPQLY
DKKVSLILNRSLARTPSTTSFKTSLADSFGNSPTTPTADARFGSSTNVSVIGNSGGPP
TPRKREIVDFGRHTRSSSLSSGSVLSSPDIDDQNMSPQDLESTGDEFKLHNGGSTVSF
TKYVTSPNSGSSKKLSGVVPRLARTKSSSSSNPSSPVQRVQSPIMRDQAEMPSAPQEN
SFRLESFLRGANIRKVKKKASKLDKVKEKDIRDDNAKEEQRQEEEPRSKSKSRNGRSS
VMTFGTKLSNSPASMSASSSVSSLTRHKDNQTGQSSYQFPPPR"
gene complement(<90163..>91146)
/gene="NOP1"
/locus_tag="AWJ20_1993"
/db_xref="GeneID:30033855"
mRNA complement(<90163..>91146)
/gene="NOP1"
/locus_tag="AWJ20_1993"
/product="rRNA methyltransferase NOP1"
/transcript_id="XM_018878915.1"
/db_xref="GeneID:30033855"
CDS complement(90163..91146)
/gene="NOP1"
/locus_tag="AWJ20_1993"
/inference="similar to AA sequence:KEGG_Orthology:K14563"
/note="Histone glutamine methyltransferase, modifies H2A
at Q105 in nucleolus; component of the small subunit
processome complex, which is required for processing of
pre-18S rRNA; ortholog of mammalian fibrillarin;
GO_component: GO:0030686 - 90S preribosome [Evidence IDA]
[PMID 12150911]; GO_component: GO:0031428 - box C/D snoRNP
complex [Evidence IDA] [PMID 11081632]; GO_component:
GO:0005730 - nucleolus [Evidence IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 11932453];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
2686980]; GO_component: GO:0005634 - nucleus [Evidence
IEA]; GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IDA] [PMID 16702403]; GO_component: GO:0032040 -
small-subunit processome [Evidence IDA] [PMID 12068309];
GO_function: GO:0003723 - RNA binding [Evidence IEA,IEA];
GO_function: GO:1990259 - histone-glutamine
methyltransferase activity [Evidence IMP] [PMID 24352239];
GO_function: GO:0008168 - methyltransferase activity
[Evidence IEA,IEA]; GO_function: GO:0008649 - rRNA
methyltransferase activity [Evidence IDA,IMP] [PMID
12215523]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0000494 - box C/D snoRNA
3'-end processing [Evidence IMP] [PMID 10733567];
GO_process: GO:0000494 - box C/D snoRNA 3'-end processing
[Evidence IMP] [PMID 15167896]; GO_process: GO:1990258 -
histone glutamine methylation [Evidence IDA,IMP] [PMID
24352239]; GO_process: GO:0032259 - methylation [Evidence
IEA]; GO_process: GO:0031167 - rRNA methylation [Evidence
IDA,IMP] [PMID 12215523]; GO_process: GO:0031167 - rRNA
methylation [Evidence IMP] [PMID 8431947]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA,IEA];
GO_process: GO:0006364 - rRNA processing [Evidence IMP]
[PMID 1825809]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA]; GO_process: GO:0043144 - snoRNA
processing [Evidence IMP] [PMID 1825809]; GO_process:
GO:0008033 - tRNA processing [Evidence IEA]"
/codon_start=1
/product="rRNA methyltransferase NOP1"
/protein_id="XP_018736882.1"
/db_xref="GeneID:30033855"
/translation="MAFTPGGRGGSRGGGRGGFGGGRGGFGGGRGGAPGGGRGGFGGG
RGGARGGAAGGRGGRGGARGGRGGPRGGRGGARGGRGGAAGGAKVVIEPHKHAGVFIA
RGKEDLLVTKNLTPGESVYGEKRVSIDVPGQGEDAVATKVEYRVWNPFRSKLAAGILG
GLDELFVGPGKKVLYLGAASGTSVSHVADVVGPEGVVYAVEFSHRSGRDLISMAKKRP
NVIPIIEDARHPQKYRMLVGMVDVVFADVAQPDQARIIALNSHLFLKDQGGVVISIKA
NCIDSTVEAETVFAREVQKLREERVKPLEQLTLEPYERDHCIVVGRYVRSS"
gene <92150..>92707
/gene="RPL17A"
/locus_tag="AWJ20_1994"
/db_xref="GeneID:30033856"
mRNA <92150..>92707
/gene="RPL17A"
/locus_tag="AWJ20_1994"
/product="ribosomal 60S subunit protein L17A"
/transcript_id="XM_018878916.1"
/db_xref="GeneID:30033856"
CDS 92150..92707
/gene="RPL17A"
/locus_tag="AWJ20_1994"
/inference="similar to AA sequence:KEGG_Orthology:K02880"
/note="Ribosomal 60S subunit protein L17A; copurifies with
the Dam1 complex (aka DASH complex); homologous to
mammalian ribosomal protein L17 and bacterial L22; RPL17A
has a paralog, RPL17B, that arose from the whole genome
duplication; protein abundance increases in response to
DNA replication stress; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11914276]; GO_component:
GO:0022625 - cytosolic large ribosomal subunit [Evidence
IDA] [PMID 18782943]; GO_component: GO:0005622 -
intracellular [Evidence IEA]; GO_component: GO:0015934 -
large ribosomal subunit [Evidence IEA]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0005840 - ribosome [Evidence IEA,IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IEA]; GO_function: GO:0003735 -
structural constituent of ribosome [Evidence IDA] [PMID
18782943]; GO_process: GO:0002181 - cytoplasmic
translation [Evidence IDA] [PMID 18782943]; GO_process:
GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="ribosomal 60S subunit protein L17A"
/protein_id="XP_018736883.1"
/db_xref="GeneID:30033856"
/translation="MTRYSTTPVNPAKSASSRGSYLRVSYKNTYETAQAVNGWPLTKA
LTYLEQVSEKKRAIPFRKHAGSIGRASQGKEFGVTKARWPVKSVKFVSDLLKNAQANA
DAKGLDTEKLVIKHIQVNQAPKQRRRTYRAHGRINPYQSSPCHIEIILTEADEEIAKA
PETSVVRLNSRQRGAKASQKRITAA"
gene complement(<93098..>95224)
/gene="COY1"
/locus_tag="AWJ20_1995"
/db_xref="GeneID:30033857"
mRNA complement(<93098..>95224)
/gene="COY1"
/locus_tag="AWJ20_1995"
/product="Coy1p"
/transcript_id="XM_018878917.1"
/db_xref="GeneID:30033857"
CDS complement(93098..95224)
/gene="COY1"
/locus_tag="AWJ20_1995"
/inference="similar to AA sequence:KEGG_Orthology:K09313"
/note="Golgi membrane protein with similarity to mammalian
CASP; genetic interactions with GOS1 (encoding a Golgi
snare protein) suggest a role in Golgi function;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IGI]
[PMID 12429822]; GO_component: GO:0030173 - integral
component of Golgi membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0048193 - Golgi vesicle transport
[Evidence IGI] [PMID 12429822]; GO_process: GO:0006891 -
intra-Golgi vesicle-mediated transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Coy1p"
/protein_id="XP_018736884.1"
/db_xref="GeneID:30033857"
/translation="MSSANNSPKIPEDDVNANSTAPTSTGGPSAPEGQSTTTTTSKEA
LNPFEQAIKVWSDIDLSSLQKSLDDEGDQILVNQTTSVKGRKELASRTKSFRKLSDEE
KLVEIKSLLKLYQTEIDNLTNRGKFAETSFMNVYKLLAEAPDPRPLLEASVDSVISAS
EVNRLMTENARLNDLVSRHADYDSVKAKLLKVEQSSIETARAKVNAKEAEMNALFDEK
ERNWKLREEELQRQIEDLSANKDVAEARLSAHNSSIDPSEGAKSRLLELELVAGDLER
ANRKVAQVEKRNLELRTELEGLKSETKANEEKAVLESKISDLEGENAIVLAKIQSLDS
KLATQTAASQQKIEVLERDVSRKSEEITTLKNRLHKQQDYEEIKRELDVLKSIEFDVG
EDDGERSARSISTQNENAQETKLEKMLLTRNKKLTSDLTNLRVTNHELNEANQAANRQ
ISSLTSELEEARNLNNKLEEDLLTLKNSGTSPAMSTVSGWNNSSWGGKNSVNGRVSPT
SSIIGGRDGRDLRGGPTPTTSSSDSSILPIITQQRDRFRQRNAELESELRKNWALISS
LQKEVETVKKDNVELYERTRYASSYKAQSKRGGSSAEDRYRSVYEEGLTPFQQFRGRE
TERALSKMGPLERIVYSFFRGVLSNRLSRNFFFLYCLGLHLLVMVILMYGATSSPVLE
VPSTVPGTGAEESLVSEAAVQLAEHP"
gene <96127..>97461
/gene="FUN26"
/locus_tag="AWJ20_1996"
/db_xref="GeneID:30033858"
mRNA <96127..>97461
/gene="FUN26"
/locus_tag="AWJ20_1996"
/product="nucleoside transmembrane transporter FUN26"
/transcript_id="XM_018878918.1"
/db_xref="GeneID:30033858"
CDS 96127..97461
/gene="FUN26"
/locus_tag="AWJ20_1996"
/inference="similar to AA sequence:KEGG_Orthology:K15014"
/codon_start=1
/product="nucleoside transmembrane transporter FUN26"
/protein_id="XP_018736885.1"
/db_xref="GeneID:30033858"
/translation="MPEDNEDNEAVYLLNEEGDVPPNLIGTNSPPSISNLDYVAYATI
GASYLWPWNCLLSATPYFQGRFEGYKFLQANFSSFVMSITTVTGTLFTILLADRQKNA
DYSWRVRVGELTIASVFLLLAFSCALSIPAVPYFLFILLCVFSSSIGTGLTQNGSYAI
GNTLTSSHTQAITVGQAVSGVLPPIISMLSAIGSGASKEHSNDVNESLDVRESGSSST
NSSVGSALYFLAATAVCTAAIICFRQSEKYRKSNASGFSFSNENPAPEPLSATKKQHI
PLSTLFSKLFVPASTVFITFAITLIYPVFAGTVKSSNGIPTQFFIPAVYLFWNSGDLL
GRVLCGRPQFVVKDRRALTGYAVARLLFVPAFFLTNINGDGIIQSDVYYLLLQFAFGV
TNGQLVTSALMESPSFVNENEKEAAGGFMTMSLSIGLAVGSLLSFVLVWILG"
gene complement(<97544..>97846)
/locus_tag="AWJ20_1997"
/db_xref="GeneID:30033859"
mRNA complement(<97544..>97846)
/locus_tag="AWJ20_1997"
/product="hypothetical protein"
/transcript_id="XM_018878919.1"
/db_xref="GeneID:30033859"
CDS complement(97544..97846)
/locus_tag="AWJ20_1997"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736886.1"
/db_xref="GeneID:30033859"
/translation="MLTTKLQSLWLLRQVIQGEGHSYELQGGKFILRLANIFLQGSYK
GLLVQVEYNSSGSEDTSGQIQKINEFLAQYGLKFVGNKLAKDEIGTAWQYVDALSR"
gene <98510..>99682
/gene="RPD3"
/locus_tag="AWJ20_1998"
/db_xref="GeneID:30033860"
mRNA <98510..>99682
/gene="RPD3"
/locus_tag="AWJ20_1998"
/product="histone deacetylase RPD3"
/transcript_id="XM_018878920.1"
/db_xref="GeneID:30033860"
CDS 98510..99682
/gene="RPD3"
/locus_tag="AWJ20_1998"
/inference="similar to AA sequence:KEGG_Orthology:K11405"
/codon_start=1
/product="histone deacetylase RPD3"
/protein_id="XP_018736887.1"
/db_xref="GeneID:30033860"
/translation="MPVAYAYSEELIEYADKLPSNKGRASRVNALIESFGLMDKFDGI
IRVKPCTQADLEKYHDKNFIKFLLTGDDGLDAESETEGEESDESYEGKDEPVSKEALY
GLHYDCPPFDGLEKYVKLVAGATLQCADYLVSLLDTDIAQNTSKVAINWTGGRHHAKR
AACSGFCYVNDINLGIQRLRTKFDKVLYVDLDLHHGDGVETAFAYSDKVKSVSVHRYE
RGFFPGTGGMNTTSRSVVNIPTKYGLSDKSFIQIFDTIIVAEKEDFQPDAVVVTTGCD
GLARDELREWNLSFSSFEYIIDQIVNSWKLPTLLLGGGGYHTLDTARCLAICTAAALN
VPDRKQWVEIPMHDYFNEYVKDNYSLRVESHVRHNTPDENSEEYLKKLADYFEHRR"
gene <100425..>101837
/gene="BFR1"
/locus_tag="AWJ20_1999"
/db_xref="GeneID:30033861"
mRNA <100425..>101837
/gene="BFR1"
/locus_tag="AWJ20_1999"
/product="Bfr1p"
/transcript_id="XM_018878921.1"
/db_xref="GeneID:30033861"
CDS 100425..101837
/gene="BFR1"
/locus_tag="AWJ20_1999"
/note="Component of mRNP complexes associated with
polyribosomes; implicated in secretion and nuclear
segregation; multicopy suppressor of BFA (Brefeldin A)
sensitivity; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 23222640]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IDA] [PMID 23222640];
GO_component: GO:0042175 - nuclear outer
membrane-endoplasmic reticulum membrane network [Evidence
IDA] [PMID 11410665]; GO_component: GO:0005844 - polysome
[Evidence IDA] [PMID 11410665]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IDA] [PMID 11410665];
GO_function: GO:0003723 - RNA binding [Evidence IDA] [PMID
11410665]; GO_function: GO:0003729 - mRNA binding
[Evidence IDA] [PMID 23222640]; GO_process: GO:0016071 -
mRNA metabolic process [Evidence IPI] [PMID 11410665];
GO_process: GO:0007126 - meiotic nuclear division
[Evidence IMP] [PMID 8913743]; GO_process: GO:0007088 -
regulation of mitosis [Evidence IMP] [PMID 8913743]"
/codon_start=1
/product="Bfr1p"
/protein_id="XP_018736888.1"
/db_xref="GeneID:30033861"
/translation="MSKSNSPTPAPAAGAGKRVTRPNKEAFNAKLAELEAEFKTAEKN
LEIARNDVKKALGTSTPQTEEREKLSNELNEIAAKQRQFKTSNASIKKEIDIIDENIK
RSIKDLKALKDKNSYKSVDEVNAKIKSLERDIESGKLRLVEEKRYIADISNLKKIKNS
FGQLDQLQASIDAERAKLAKVRGTLDTTSQNALSDRYNEVKKQLDAIRDGQQKAYKEK
NTLFEKRDAANKVRDAARSNISKLKDEYYGQLRAYTAQAEADRIARAEKEEAEKKAFA
AEKKKAELREKLEAASEPAFANQIDIAENLLVHFDPEYKRADSNDISLKSTGPVKTAS
GRTIDVPHGAELKKSDEELFAGLGGKKNKKKSSSDSKLRLNVGIIDDFATLNIPIPVS
KDDVEGTIAKLKEKIQYYKENQDRVTAERIERAKAEIAKLELADKEDEEKKEEEEEKA
EEAPAATEKAEPEASVEASA"
gene complement(<101977..>103020)
/gene="FEN2"
/locus_tag="AWJ20_2000"
/db_xref="GeneID:30033865"
mRNA complement(<101977..>103020)
/gene="FEN2"
/locus_tag="AWJ20_2000"
/product="Fen2p"
/transcript_id="XM_018878925.1"
/db_xref="GeneID:30033865"
CDS complement(101977..103020)
/gene="FEN2"
/locus_tag="AWJ20_2000"
/inference="similar to AA sequence:KEGG_Orthology:K03448"
/note="Plasma membrane H+-pantothenate symporter; confers
sensitivity to the antifungal agent fenpropimorph;
relocalizes from vacuole to cytoplasm upon DNA replication
stress; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 22842922]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence IEA,IEA];
GO_component: GO:0005886 - plasma membrane [Evidence
IGI,ISS] [PMID 10373490]; GO_function: GO:0015233 -
pantothenate transmembrane transporter activity [Evidence
IGI,IMP] [PMID 10373490]; GO_process: GO:0006897 -
endocytosis [Evidence IMP] [PMID 15020461]; GO_process:
GO:0015887 - pantothenate transmembrane transport
[Evidence IGI,IMP] [PMID 10373490]; GO_process: GO:0055085
- transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Fen2p"
/protein_id="XP_018736889.1"
/db_xref="GeneID:30033865"
/translation="MLGSWYKKNELGKRAAIFVSVGDIGSMAGGWIQAGLFARLDGHG
LPAWRLIFIVVFAMTIPFVFLGLIAIPDLPRHENAWFLSDEEKKLAIDRLQRHVPTET
MSLRVVWRAVKSWQFWLMPFIFFLYGVSVQMLGNNVMPLWMKSRGYTVVQMNNYPTVI
FAFAIVGTLGYSFLSDRLGSRWQASVAIGFTFTLCCPILLSNNVPDGAKFFAFFFLGT
CLAPQALWFTWCADVTSHDLYLRSITTGWMNAIDEAFIAWWPIVFYPVTDAPTFRTGY
IASLVLGTLIVPTVGAIVYLDRRDQRLHYDADSDDEQTPIEGVGHNSSQRSILANDAS
KLQSNYNSIEVTI"
gene <104711..>107242
/locus_tag="AWJ20_2001"
/db_xref="GeneID:30033866"
mRNA <104711..>107242
/locus_tag="AWJ20_2001"
/product="hypothetical protein"
/transcript_id="XM_018878926.1"
/db_xref="GeneID:30033866"
CDS 104711..107242
/locus_tag="AWJ20_2001"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736890.1"
/db_xref="GeneID:30033866"
/translation="MGQQVTGANDLGLNIAVESHGRFIAVSDSNKRVRIMAFGLSDDN
SLASIIDSRQDIQSRHTISIGGGVVLNMAFLTTGSTAYNTSTSMSPKIAPVASQGDPR
TTGGDDVYLVLLYAVSRKLKLNMYKVPYGQREFSYIHDIGLCGTFTLSLDFKEEIPMY
MVPVSMNESSAIVLVTKRQLLLVTFLDIMSGHRILTSALLPDYPLAVHQSPYNKDRSV
FYLTTKDSHLYRVKVSEMRGFELLSFKHWPGRDLGSSFVLQPYDDEGPTSDINSDRLL
ITFGGDLCCGGSMVFNSVGMIYDDGEQADIEEEGDVDIYDNWAPISDFKPINIQSTSR
GDSLELFACTGLHHQGAITQFRHGIRASVFLQGPSMTEAPQLYSMQSHDGLESYLIVS
LPWKTTLYLVEQSTIDEQNGQEAYFPQLSEVPNNCGIDYKNETKAISFLPETKFVIQV
TSSQILLTDLMGSSKRFDVTRASCAAIFKSMVAVAFYDEDALSNKVVIYSINSVNYED
GDNWIERKSVVLMSHPVSFVKFTERKDNGINLFIGSYEPRIDVYHIDNDCDARLVLEI
KNIFTDEEEVPNDLHLQDRMLDRFIIGLRNGSYINCVLEDMTMRLESKKKLANLPIQF
VNIDHKPGPNVFVLSNGRLSCFDKKNFLEAPQKVVVDIDKVADIRSACQFYYNMEDGK
HQFVAVIMNNRLTILALDLFATVIPKQVHLGASPRRMLYLEHIGALAVVVSPSSTSKT
SLLRFVDPKRMVEIDFRLYDATSPSSKTCFQANEVIYSLNEWSLKIDGNTYKFLLLGS
GIKGLPEGKFRILRLSRTTEGIKATIQHSWITAGHVYSVSQFEAL"
gene <109047..>112073
/gene="PET309"
/locus_tag="AWJ20_2002"
/db_xref="GeneID:30033867"
mRNA <109047..>112073
/gene="PET309"
/locus_tag="AWJ20_2002"
/product="Pet309p"
/transcript_id="XM_018878927.1"
/db_xref="GeneID:30033867"
CDS 109047..112073
/gene="PET309"
/locus_tag="AWJ20_2002"
/note="Specific translational activator for the COX1 mRNA;
also influences stability of intron-containing COX1
primary transcripts; localizes to the mitochondrial inner
membrane; contains seven pentatricopeptide repeats (PPRs);
GO_component: GO:0031314 - extrinsic component of
mitochondrial inner membrane [Evidence IDA] [PMID
18039654]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence IDA] [PMID
9692914]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IEA,IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IDA] [PMID
9692914]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0045182 -
translation regulator activity [Evidence IGI] [PMID
7664742]; GO_process: GO:0016070 - RNA metabolic process
[Evidence IMP] [PMID 7664742]; GO_process: GO:0009060 -
aerobic respiration [Evidence IMP] [PMID 7664742];
GO_process: GO:0097034 - mitochondrial respiratory chain
complex IV biogenesis [Evidence IMP] [PMID 7664742];
GO_process: GO:0070134 - positive regulation of
mitochondrial translational initiation [Evidence IMP]
[PMID 18039654]; GO_process: GO:0070134 - positive
regulation of mitochondrial translational initiation
[Evidence IMP] [PMID 7664742]"
/codon_start=1
/product="Pet309p"
/protein_id="XP_018736891.1"
/db_xref="GeneID:30033867"
/translation="MSWSGILRSSSSNTLRRSEALYWSLQSYSQAYFSGSWNRYLLAA
SIVWENGLFGATTKDTRKRLNKTRVGKTHLEKFWHQKRKLSSWSPSCASAVNITSSRS
PEFAGSASIGQISKGPEMGAVPRDLINALNPKNLSDLELIESWRKEITSTDRRRQMQA
LAKKQELYSHFLSSLQRTPNEDLLEQLLALVHCVPHPHSSVDYNFRIYLLRISGELDM
ISCCLEEASNQLSKDEFLAVLRAVVDDFISSHTPIPPSFLNIFASTILKYDGTLELLS
RRYHEERSILKSIIRHLSTASPTLTSILDDFLRNSPHFGSMLVTTCIDILGQPEDVKP
QLVEQLWNRKLELDLASPLDLHRVMTAFYHNCQYEQVLHLYDSYPSLHMHWQFDIVLL
TLAKLRNWDRLQTVFESLFRHDDLPDINHYGIVIQALSQLGKVDIVDTLFSSLLAKSI
NPSIEILHGLMYSRLALGEVHGVEYFFNQITILGLRPNPRSYNILLRSYRDSHEIQKA
LNLIGQMAELNIPITRYAVTTLISTCTERRDTETGEALFDWALSYGIKPDIIMFNSLI
ACYMESNCLTKAEKIYEMMKEGSHCRPTVDTLTSMLTGYSRMATKIPNSQQKVLKLEE
DMKKYSISPDDRWLSSLMHYNLSIKNFKQVEAIFVDLTKKYRVDTTPYHYSIMMEAYI
IQRKLKEAEELYNTMKQQNVIPTFNTQEKYLRLKISDPAAKVRQEAAGYLQDFLQATD
IIDLESKQLPRNVIPFSMVKRVSREFMKSGNYESALEFMKPYNEEGYDPKEQFKVSAF
MLELYGHAGYWSLIEKTWESFLEQVRRAYVPVKVSDGAEPRYEKRLPTRYRGTFWQAV
NSKLNQLQNFGQYSKMLDLISWAEKEGLGVTSKNMNTLVKGLLFDDKFLVKAFEVSEE
CLAPTTVAKLQAKGSGHTHPLYGLKYKDYNLSMSTFKALVRKFDRLITITAYEGKCSR
LKAFILVNEKYPKLMKLFSWREKSRTKRRPRLAAST"
gene complement(<112221..>112631)
/gene="SPC3"
/locus_tag="AWJ20_2003"
/db_xref="GeneID:30033868"
mRNA complement(<112221..>112631)
/gene="SPC3"
/locus_tag="AWJ20_2003"
/product="Spc3p"
/transcript_id="XM_018878928.1"
/db_xref="GeneID:30033868"
CDS complement(112221..112631)
/gene="SPC3"
/locus_tag="AWJ20_2003"
/inference="similar to AA sequence:KEGG_Orthology:K12948"
/note="Subunit of signal peptidase complex; complex
catalyzes cleavage of N-terminal signal sequences of
proteins targeted to the secretory pathway; homologous to
mammalian SPC22/23; other members of the complex are
Spc1p, Spc2p, and Sec11p; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005789 - endoplasmic reticulum membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA,IEA]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005787 - signal
peptidase complex [Evidence IEA]; GO_component: GO:0005787
- signal peptidase complex [Evidence IDA] [PMID 1846444];
GO_component: GO:0005787 - signal peptidase complex
[Evidence IDA] [PMID 8910564]; GO_function: GO:0008233 -
peptidase activity [Evidence IEA]; GO_function: GO:0008233
- peptidase activity [Evidence IMP] [PMID 9148930];
GO_function: GO:0008233 - peptidase activity [Evidence
IMP] [PMID 9148931]; GO_process: GO:0045047 - protein
targeting to ER [Evidence IMP] [PMID 9148931]; GO_process:
GO:0006508 - proteolysis [Evidence IEA]; GO_process:
GO:0006465 - signal peptide processing [Evidence IEA];
GO_process: GO:0006465 - signal peptide processing
[Evidence IMP] [PMID 9148930]; GO_process: GO:0006465 -
signal peptide processing [Evidence IDA,IMP] [PMID
9148931]"
/codon_start=1
/product="Spc3p"
/protein_id="XP_018736892.1"
/db_xref="GeneID:30033868"
/translation="MSRKVAARFGRRGGSVDGKAKDNYRIKFDLSTDLSPLFNWNTKQ
VFVYLTAEYDGKREDINNRVTFWDSIIKSKEDAVLDLKNVRGSYSVYDVGSSLENKNA
TIRLEWNVQPHVGFLIFGETVATEDGSFIFPALT"
gene <115250..>116755
/gene="UGA2"
/locus_tag="AWJ20_2004"
/db_xref="GeneID:30033869"
mRNA <115250..>116755
/gene="UGA2"
/locus_tag="AWJ20_2004"
/product="succinate-semialdehyde dehydrogenase
(NAD(P)(+))"
/transcript_id="XM_018878929.1"
/db_xref="GeneID:30033869"
CDS 115250..116755
/gene="UGA2"
/locus_tag="AWJ20_2004"
/inference="similar to AA sequence:KEGG_Orthology:K00135"
/note="Succinate semialdehyde dehydrogenase; involved in
the utilization of gamma-aminobutyrate (GABA) as a
nitrogen source; part of the 4-aminobutyrate and glutamate
degradation pathways; localized to the cytoplasm;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_function: GO:0016620 -
oxidoreductase activity, acting on the aldehyde or oxo
group of donors, NAD or NADP as acceptor [Evidence IEA];
GO_function: GO:0009013 - succinate-semialdehyde
dehydrogenase [NAD(P)+] activity [Evidence IEA,IEA];
GO_function: GO:0009013 - succinate-semialdehyde
dehydrogenase [NAD(P)+] activity [Evidence IMP,ISS] [PMID
11031268]; GO_function: GO:0009013 -
succinate-semialdehyde dehydrogenase [NAD(P)+] activity
[Evidence IMP] [PMID 3888627]; GO_process: GO:0034599 -
cellular response to oxidative stress [Evidence IMP] [PMID
11031268]; GO_process: GO:0009450 - gamma-aminobutyric
acid catabolic process [Evidence IEA,IEA]; GO_process:
GO:0009450 - gamma-aminobutyric acid catabolic process
[Evidence IEP,IMP,ISS] [PMID 11031268]; GO_process:
GO:0009450 - gamma-aminobutyric acid catabolic process
[Evidence IMP] [PMID 3888627]; GO_process: GO:0006540 -
glutamate decarboxylation to succinate [Evidence
IGI,IMP,ISS] [PMID 11031268]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="succinate-semialdehyde dehydrogenase
(NAD(P)(+))"
/protein_id="XP_018736893.1"
/db_xref="GeneID:30033869"
/translation="MLRSRIFTRAMSSTGKPVLKNPKLLRDKSYVDGQWISASSGKTF
KVFDPATCQEIGSVPEHTAADYAKAIEHAKKAFASFSKTSNRERSNLLRKWASLLTEN
SEDLAKILTWENGKPLPEAKGEIASCVANFEWFSEEAPRIYGDTIPSQNPNTRIHTIK
QPVGVCGIITPWNFPASMISRKVGAAVATGCTTVIKPAAETPYSALALAYLAEEAGIP
KGVVNVLTTNEHVQDVGREICENPLVSKVTFTGSTNVGRILMKQAASTLKKVSFELGG
NAPFIVFEDADIDAAVDGAIASKFRGSGQTCICANRFYVHEKIYDEFAKKLAAKVSGF
KVGNGLEEGTTQGPLINEKAVQKVDRHVQDALGKHAHALVGGKPATELGENFYSPTVL
VDVDASMAVTQEETFGPLAALSKFSTDEEVIKLANNVEVGLAAYIYTNNNSRVHRVSE
GLQVGMVGVNTGLITESALPFGGVKESGFGREGSKYGLDDYLVIKSVVNAI"
gene complement(<115696..>116136)
/locus_tag="AWJ20_2005"
/db_xref="GeneID:30033870"
mRNA complement(<115696..>116136)
/locus_tag="AWJ20_2005"
/product="hypothetical protein"
/transcript_id="XM_018878930.1"
/db_xref="GeneID:30033870"
CDS complement(115696..116136)
/locus_tag="AWJ20_2005"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736894.1"
/db_xref="GeneID:30033870"
/translation="MAPSTAASMSASSNTMKGALPPNSKETFLRVDAACFMRILPTLV
EPVKVTLLTNGFSQISLPTSWTCSLVVNTLTTPLGIPASSARYANANAEYGVSAAGLM
TVVQPVATAAPTFLEIIEAGKFQGVMIPQTPTGCLIVWIRVLGF"
gene complement(<116820..>118130)
/locus_tag="AWJ20_2006"
/db_xref="GeneID:30033871"
mRNA complement(<116820..>118130)
/locus_tag="AWJ20_2006"
/product="hypothetical protein"
/transcript_id="XM_018878931.1"
/db_xref="GeneID:30033871"
CDS complement(116820..118130)
/locus_tag="AWJ20_2006"
/inference="similar to AA sequence:KEGG_Orthology:K11979"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736895.1"
/db_xref="GeneID:30033871"
/translation="MPYDPNHCTHHDAPMRQQLFACLTCRAKDKQDNGICYSCSIQCH
SNHELVELFARRGFSCDCGTTRMASFGACNLRKNFDGLDTPEETNKYNHNFSGRFCSC
DQVYDPSSEQGIMFQCLLGDACDEEWFHEECILGVDRLGTVETEANPESTSVKDDAID
DTALKSHYPQGVNMYDLLEPAESSVVKAPEEIKEEQEKSEQNQDGNLAVNNEEDDEDE
EGDEDDEDDTLDGLPRQEDIGAFICWKCVEKNPILLKWGKSWPEVAHEPVVHGIFKTI
EERELSIKSLKPEPSNRDVEKNEFDKRALDDGVSSIQPLKKIKSDEQKQEVSLFLKPG
FHDHVKASSDPDIKAFAEKFPFLLEEESVYEPPQDDDANSSLLDAGTRALNSIPRDQA
INGMQAYSMIRERLSLFLRPFAEQGKVVTEHDVTNFFEEVRNEK"
gene <118446..>119633
/gene="RAD57"
/locus_tag="AWJ20_2007"
/db_xref="GeneID:30033872"
mRNA <118446..>119633
/gene="RAD57"
/locus_tag="AWJ20_2007"
/product="putative DNA-dependent ATPase RAD57"
/transcript_id="XM_018878932.1"
/db_xref="GeneID:30033872"
CDS 118446..119633
/gene="RAD57"
/locus_tag="AWJ20_2007"
/inference="similar to AA sequence:KEGG_Orthology:K10958"
/codon_start=1
/product="putative DNA-dependent ATPase RAD57"
/protein_id="XP_018736896.1"
/db_xref="GeneID:30033872"
/translation="MDLYESYPSCSLLKNTNFDSLLSAFEEFEITTADLVVNDPKVIA
QRCGRSQVELLRFLNIFYKEALNGLEFINIGELQDGKLDKNTACLSGTSSSPLQESCI
STRMLTTGDSKFDSELRGGIPAGYITEVSGESGSGKSNFLTQLCVTVQLPKALGGLEK
SALYICTESGFETRRLIDMIEYIKQTHSLNESQISSDNVHCINCRDQEHLEQVLKYQL
PVAIERFNVGILLIDSIAAHLRAEFDLQHVQQRNNKLILLSRHLRKLASLNELAVVIA
NQVSDRFIRNLPAHSEPDALFLDHQIRWFSGWEEPNIVVPPSSSFHSSQQSTPATTER
PRTPSLGLAWSNCIDQRIVLKRALLPDGSSHRTFNIVFSPFSPQSCTPFKIMKGGVYS
IDT"
gene <119897..>122404
/gene="MCM2"
/locus_tag="AWJ20_2008"
/db_xref="GeneID:30033873"
mRNA <119897..>122404
/gene="MCM2"
/locus_tag="AWJ20_2008"
/product="MCM DNA helicase complex subunit MCM2"
/transcript_id="XM_018878933.1"
/db_xref="GeneID:30033873"
CDS 119897..122404
/gene="MCM2"
/locus_tag="AWJ20_2008"
/inference="similar to AA sequence:KEGG_Orthology:K02540"
/note="Protein involved in DNA replication; component of
the Mcm2-7 hexameric helicase complex that binds chromatin
as a part of the pre-replicative complex; relative
distribution to the nucleus increases upon DNA replication
stress; GO_component: GO:0031261 - DNA replication
preinitiation complex [Evidence IDA] [PMID 9554851];
GO_component: GO:0042555 - MCM complex [Evidence IEA];
GO_component: GO:0042555 - MCM complex [Evidence IDA]
[PMID 12480933]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 10704410]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 22842922]; GO_component:
GO:0005656 - nuclear pre-replicative complex [Evidence
IDA] [PMID 16824194]; GO_component: GO:0005656 - nuclear
pre-replicative complex [Evidence IDA] [PMID 9335335];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
10704410]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 22842922]; GO_component: GO:0031298 -
replication fork protection complex [Evidence IDA] [PMID
16531994]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0003677 - DNA binding [Evidence
IEA,IEA]; GO_function: GO:0003678 - DNA helicase activity
[Evidence IEA]; GO_function: GO:0003678 - DNA helicase
activity [Evidence IDA] [PMID 18657510]; GO_function:
GO:0003688 - DNA replication origin binding [Evidence IDA]
[PMID 11756674]; GO_function: GO:0003688 - DNA replication
origin binding [Evidence IDA] [PMID 16824194];
GO_function: GO:0003682 - chromatin binding [Evidence IDA]
[PMID 10783164]; GO_function: GO:0004386 - helicase
activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0003697 - single-stranded DNA binding
[Evidence IMP] [PMID 17895243]; GO_function: GO:0017116 -
single-stranded DNA-dependent ATP-dependent DNA helicase
activity [Evidence IDA] [PMID 18657510]; GO_process:
GO:0032508 - DNA duplex unwinding [Evidence IEA];
GO_process: GO:0006260 - DNA replication [Evidence
IEA,IEA]; GO_process: GO:0006270 - DNA replication
initiation [Evidence IEA]; GO_process: GO:0006271 - DNA
strand elongation involved in DNA replication [Evidence
IMP] [PMID 10834843]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0006974 - cellular response
to DNA damage stimulus [Evidence IMP] [PMID 22564307];
GO_process: GO:0000727 - double-strand break repair via
break-induced replication [Evidence IMP] [PMID 20516198];
GO_process: GO:1902450 - negative regulation of
ATP-dependent DNA helicase activity [Evidence IDA] [PMID
13679365]; GO_process: GO:0033260 - nuclear cell cycle DNA
replication [Evidence IMP] [PMID 10834843]; GO_process:
GO:0006267 - pre-replicative complex assembly involved in
nuclear cell cycle DNA replication [Evidence IDA] [PMID
16824194]; GO_process: GO:0006267 - pre-replicative
complex assembly involved in nuclear cell cycle DNA
replication [Evidence IPI] [PMID 9335335]"
/codon_start=1
/product="MCM DNA helicase complex subunit MCM2"
/protein_id="XP_018736897.1"
/db_xref="GeneID:30033873"
/translation="MSGSRKRDRRNSEDFRGEPPSSPNLPMANLASSPPAFEDEDELI
QEDVIDDLDELAEEEAGEDLFGDNMERDYMESRQNDQYDLGDQNIDDNEYDDLDISAR
RQVDAQLNRRDRQIRNQRGLPDAFLDDGDGYDDLVPPTTRRRRNQYDEDLAMDNSDDD
DPMNEILSLESLGDVKAESIAEWVSQPSVHRSVAREFKNFLLEYTDENGRSVYGNRIR
TLGEVNSESLEIVYTHLVESKATLAYFLAVAPTEILKIFDVVAMEAIELHYPEYSRIH
AEVHVRISGLPTTFTLRDLRENHLNSLVRVTGVVTRRTGVFPQLKYVKFNCPKCGMIL
GPYFQDSNTEVTISFCHSCQSRGPFILNSEKTVYRNYQKITLQESPGTVPAGRLPRHR
EVILLWDLIDVAKPGEEVDITGIYKNSYDGGLNAKNGFPVFATVIEANLVQRREAGKT
GDFTLTEEEEREIRTLAKSKDIINTIIRSIAPSIYGHKDIKTALACSLFGGVAKRQEK
HTVRGDINVLLLGDPGTAKSQLLKYVEKTAHRAVFATGQGASAVGLTASVRKDPITRE
WTLEGGALVLADKGTCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCAV
IAAANPNGGRYNSTIPFAANVDLTEPILSRFDVLCVVRDTVDPEVDEMLAKFVVTSHT
KNHPLDPGEGNSGLQQEDEDDDMGEEEEEDLLASLQPNAGATISQELLRKYIHYARTK
FQPRLSQQYEGKIGSIYSEIRQYSRNTGSFPITVRHLESIIRLSESFAKMRLSENVSE
GDVNRAISVTIDSFVGAQKSSLRRSLRRSLMKYTYNN"
gene complement(<122694..>123470)
/gene="MAF1"
/locus_tag="AWJ20_2009"
/db_xref="GeneID:30033874"
mRNA complement(<122694..>123470)
/gene="MAF1"
/locus_tag="AWJ20_2009"
/product="RNA polymerase III-inhibiting protein MAF1"
/transcript_id="XM_018878934.1"
/db_xref="GeneID:30033874"
CDS complement(122694..123470)
/gene="MAF1"
/locus_tag="AWJ20_2009"
/codon_start=1
/product="RNA polymerase III-inhibiting protein MAF1"
/protein_id="XP_018736898.1"
/db_xref="GeneID:30033874"
/translation="MSPPTLQVFSPPSSYYPTSNVHEHVFDPNSKENVLSRSRRNSRA
NSGYAGSLGTHTSNYNSGGNGTLTVSNIIQNSTAGSLSSSPFGPLDQAASRKTFAYLI
SVLNASDPDRDFSSLQPEDFKREPSSTSVVNTFNNILFGQGLPVPPRLWETLDKNIDL
KESSIYSHTPPEAFLADEPGTMWSMMWFFFNKRRKRVAYIHLKAVRHYQSPLLTAVDS
RRRRKDEIKDEYDEDKDEYDLTYSSDSQMYDEVVGDLELE"
gene complement(<124618..>125844)
/gene="PPH3"
/locus_tag="AWJ20_2010"
/db_xref="GeneID:30033876"
mRNA complement(<124618..>125844)
/gene="PPH3"
/locus_tag="AWJ20_2010"
/product="phosphoprotein phosphatase PP4 catalytic subunit
PPH3"
/transcript_id="XM_018878936.1"
/db_xref="GeneID:30033876"
CDS complement(124618..125844)
/gene="PPH3"
/locus_tag="AWJ20_2010"
/note="Catalytic subunit of protein phosphatase PP4
complex; active complex is composed of Pph3p and Psy2p,
with Psy4p apparently providing additional substrate
specificity in some cases; regulates recovery from the DNA
damage checkpoint and also the gene conversion- and
single-strand annealing-mediated pathways of meiotic
double-strand break repair; involved in activation of
Gln3p to alleviate nitrogen catabolite repression; Pph3p
and Psy2p localize to foci on meiotic chromosomes;
GO_component: GO:0000794 - condensed nuclear chromosome
[Evidence IDA] [PMID 20951350]; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_component: GO:0030289 - protein phosphatase 4 complex
[Evidence IDA,IMP] [PMID 16299494]; GO_component:
GO:0030289 - protein phosphatase 4 complex [Evidence IDA]
[PMID 17517611]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA,IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0004721
- phosphoprotein phosphatase activity [Evidence IEA,IEA];
GO_function: GO:0004722 - protein serine/threonine
phosphatase activity [Evidence IDA,IMP] [PMID 16299494];
GO_function: GO:0004722 - protein serine/threonine
phosphatase activity [Evidence IDA] [PMID 7941742];
GO_process: GO:0000724 - double-strand break repair via
homologous recombination [Evidence IGI] [PMID 21135129];
GO_process: GO:2000002 - negative regulation of DNA damage
checkpoint [Evidence IMP] [PMID 16299494]; GO_process:
GO:2000002 - negative regulation of DNA damage checkpoint
[Evidence IMP] [PMID 17517611]; GO_process: GO:1902660 -
negative regulation of glucose mediated signaling pathway
[Evidence IMP] [PMID 24277933]; GO_process: GO:0051173 -
positive regulation of nitrogen compound metabolic process
[Evidence IMP] [PMID 10940301]; GO_process: GO:0006470 -
protein dephosphorylation [Evidence IDA,IMP] [PMID
16299494]; GO_process: GO:0006470 - protein
dephosphorylation [Evidence IDA] [PMID 7941742];
GO_process: GO:0072462 - signal transduction involved in
meiotic recombination checkpoint [Evidence IMP] [PMID
20951350]"
/codon_start=1
/product="phosphoprotein phosphatase PP4 catalytic subunit
PPH3"
/protein_id="XP_018736899.1"
/db_xref="GeneID:30033876"
/translation="MSLVDETIELLKSCSHVPESVVKQLCYKAQELLIEEGNVQLVHT
PVTICGDIHGQFHDLLTLFSIGGECPTTSYLFLGDFVDRGFYSLESFLLLLCLKVRYP
DSITLIRGNHESRQITSAYGFYDECLRKYGSASVWRLCCNVFDYLSLSALVGGPGGVF
CVHGGLSPAIQNLNEIRHIDRKQEVPHDGPMCDLLWSDPDDSLDMTNENKDDIYDNEL
NENGNGHDADEEDAGTNTNYRVNHAGSGNGTGSSGESSEGMRSDSNSNDEDIYSPVSS
PDIGLNGLYDLSKSGGNRSTLQSMAASWGVSPRGAGYLFGSLPVHQFNHINNTSLIAR
AHQLVLEGYKEMFDNQLVTVWSAPNYCYRCGNVAAILNIDDNYERRYSIFHAQEADQG
TGQVVPTKRPVAEYFL"
gene <129665..>132304
/gene="RSN1"
/locus_tag="AWJ20_2011"
/db_xref="GeneID:30033877"
mRNA <129665..>132304
/gene="RSN1"
/locus_tag="AWJ20_2011"
/product="Rsn1p"
/transcript_id="XM_018878937.1"
/db_xref="GeneID:30033877"
CDS 129665..132304
/gene="RSN1"
/locus_tag="AWJ20_2011"
/note="Membrane hypothetical protein; overexpression
suppresses NaCl sensitivity of sro7 mutant cells by
restoring sodium pump (Ena1p) localization to the plasma
membrane; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 19001347]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA,IEA];
GO_component: GO:0016020 - membrane [Evidence IDA] [PMID
17005914]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006893 - Golgi to plasma
membrane transport [Evidence IGI] [PMID 17005914]"
/codon_start=1
/product="Rsn1p"
/protein_id="XP_018736900.1"
/db_xref="GeneID:30033877"
/translation="MGGSSSSSTSSGSTIGTTAVFNIAVFVIFVTAFLILRPRHKNVY
QPRVIAKTVPDSSRPRPLATGNFVWFRDLLSRPDSEILRDAGLDGYFFLRYLRLIFFI
CLVGIILVYPVLLAVNATGGGTPQGVSNSGFDILSFVNVRNKNRYFAHVFIGYLFFGF
ILFSLYRELQYYVAVRQSVLTSPAYSNLVSSRTILLTTLPDEYMNSSKLANLFEGVKF
VFINKNAKDLAKKVKQRGKLAGNIESAEVSLLKKAVSNRLKSEKKGASPIEGEDIDVY
VPQKKRPTRRLKPIIGKKVDTIDHDSQELQEVNTEIEQLQSKYTSLPSLNSAFITFHT
QEQAELGYQSLAHQQALHMAPRYIGITPDDVIWSNLRLKWWERLIKELGTTAIICALI
IFWSIPVAFVGALSNIKALEKKLPWLDFLNNLPKPLMGLVTGLLPTILLAVLMMLLPI
FIRLMGKLAGNPSKSRVEYYTQNAYFGFQVVQVFLVTTIASGASAVVVQIIEDPSSAM
SLLATNLPKASNFYIAYFMLQGFTACGSMLLQIVSLILFYVLGFILDGTPRKKWNRNN
VIGSLGWGTVFPVYTNLAVITITYSIISPLLLAFSGITFGLIYLAYLHNLMFVKSPSD
GRGIYYARAILQTFTGLYIAEVCLLGLFLLAKSWGPLVLQAIFLGFTVFVNLSLKSAF
SPLLFQLPRSLLRAEARDDNRKYSRSSAGSVIPSPDTSFEKNHNDAHPSSYPLTNEPS
NAPIVNQSQVEAGRTKYGPIPSGGTIARFFKPHLYLTPEVLNQELLNAPVFHEPQAPL
SDEEESVAYNHPAANAPNPVVWIPRDPWGLSAAQVQSLRERDVNVSDDGTWFDIDETK
KKSKFGFTSSISEVPIWTQPPNY"
gene <132768..>133616
/gene="IPP1"
/locus_tag="AWJ20_2012"
/db_xref="GeneID:30033878"
mRNA <132768..>133616
/gene="IPP1"
/locus_tag="AWJ20_2012"
/product="inorganic diphosphatase IPP1"
/transcript_id="XM_018878938.1"
/db_xref="GeneID:30033878"
CDS 132768..133616
/gene="IPP1"
/locus_tag="AWJ20_2012"
/inference="similar to AA sequence:KEGG_Orthology:K01507"
/note="Cytoplasmic inorganic pyrophosphatase (PPase);
homodimer that catalyzes the rapid exchange of oxygens
from Pi with water, highly expressed and essential for
viability, active-site residues show identity to those
from E. coli PPase; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0004427 - inorganic diphosphatase
activity [Evidence IEA,IEA]; GO_function: GO:0004427 -
inorganic diphosphatase activity [Evidence IDA] [PMID
8224193]; GO_function: GO:0000287 - magnesium ion binding
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_process: GO:0006796 -
phosphate-containing compound metabolic process [Evidence
IEA]; GO_process: GO:0006796 - phosphate-containing
compound metabolic process [Evidence IC] [PMID 8224193]"
/codon_start=1
/product="inorganic diphosphatase IPP1"
/protein_id="XP_018736901.1"
/db_xref="GeneID:30033878"
/translation="MSTSPYGIRKIGAQNTLEHKVYIEKDGKPISAFHDVPLYANAEK
TILNMIVEVPRWTNAKMEISKELPLNPIVQDTKKGKLRFVRNCFPHKGYIHNYGAFPQ
TWEDPNFTHPETKAVGDNDPLDVCEIGEAVGYTGQIKQVKVLGVMALLDEGETDWKII
AIDVKDPHASKVNDIEDVEKNFPGLLRATNEWFRIYKIPDGKPENQFAFSGECKNKKY
AEDVIRECAEAWEKLVAGKVDPKGIELSTAESEAASAVATGENLPPAPIDASIDKWFF
IAGASV"
gene complement(<134501..>136195)
/gene="TRP1"
/locus_tag="AWJ20_2013"
/db_xref="GeneID:30033879"
mRNA complement(<134501..>136195)
/gene="TRP1"
/locus_tag="AWJ20_2013"
/product="phosphoribosylanthranilate isomerase TRP1"
/transcript_id="XM_018878939.1"
/db_xref="GeneID:30033879"
CDS complement(134501..136195)
/gene="TRP1"
/locus_tag="AWJ20_2013"
/inference="similar to AA sequence:KEGG_Orthology:K01817"
/note="Phosphoribosylanthranilate isomerase; catalyzes the
third step in tryptophan biosynthesis; in 2004, the
sequence of TRP1 from strain S228C was updated by changing
the previously annotated internal STOP (TAA) to serine
(TCA); GO_component: GO:0005737 - cytoplasm [Evidence IDA]
[PMID 14562095]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016853 -
isomerase activity [Evidence IEA]; GO_function: GO:0004640
- phosphoribosylanthranilate isomerase activity [Evidence
IEA,IEA]; GO_function: GO:0004640 -
phosphoribosylanthranilate isomerase activity [Evidence
IDA] [PMID 3286643]; GO_function: GO:0004640 -
phosphoribosylanthranilate isomerase activity [Evidence
IMP] [PMID 348687]; GO_process: GO:0009073 - aromatic
amino acid family biosynthetic process [Evidence IEA];
GO_process: GO:0008652 - cellular amino acid biosynthetic
process [Evidence IEA]; GO_process: GO:0008152 - metabolic
process [Evidence IEA]; GO_process: GO:0000162 -
tryptophan biosynthetic process [Evidence IEA,IEA];
GO_process: GO:0000162 - tryptophan biosynthetic process
[Evidence IMP] [PMID 10790693]; GO_process: GO:0000162 -
tryptophan biosynthetic process [Evidence IDA] [PMID
3286643]; GO_process: GO:0000162 - tryptophan biosynthetic
process [Evidence IMP] [PMID 348687]; GO_process:
GO:0006568 - tryptophan metabolic process [Evidence IEA]"
/codon_start=1
/product="phosphoribosylanthranilate isomerase TRP1"
/protein_id="XP_018736902.1"
/db_xref="GeneID:30033879"
/translation="MADIAIFNDKARCPVDEQRRIFSQSRNCTPSSPFIVGHLNHSHT
EPDDFIQFSQIGASVLVNCSGITLHNLAARNAINRLAVRPLLLWASESDKTQEYIVGE
EVEAAVTGSDGILYHSSDLFRNDNPETIDFAVSKFQQCKAQALSSIVTIENHNHLTAF
LSLPIKPTAVFTFDDKELAASVSSALQGSVAHVHLQTITDQNPYNPSNNNFSAIFIED
VQNIPKMDAHLVQVPTHSSSKSAKSTISTSITPSSSDNSPLVKVCGIKTVEAAIKALE
SGADMIGMILVPGRSRTVDFQDAKKISDAVHNYGRAPAILPSPSPVSQTSQPSRRLSV
ISSTTSLFQKATHLSNKDTSSLPNSLNSSSSTSISQHPGSPISRRGSTQTLLPPWPSL
TNIPSGASVFEVNSRIVRAKTKRRPAIVGVFQNQPLETILSLQHELDLDYVQLHGDEP
LEWCRIIPVPVIKRFTPNTDGFANSSLVGYHYISLLDGEIGGEGKLVDWSSAKSQVAL
GARFILAGGLNESNVASALQTEGVFGVDVSGGVETAGTKDLNKIERFVKTAKAVNL"
gene <137434..>138573
/gene="SUA5"
/locus_tag="AWJ20_2014"
/db_xref="GeneID:30033880"
mRNA <137434..>138573
/gene="SUA5"
/locus_tag="AWJ20_2014"
/product="Sua5p"
/transcript_id="XM_018878940.1"
/db_xref="GeneID:30033880"
CDS 137434..138573
/gene="SUA5"
/locus_tag="AWJ20_2014"
/inference="similar to AA sequence:KEGG_Orthology:K07566"
/note="Protein involved in threonylcarbamoyl adenosine
biosynthesis; Sua5p and Qri7p are necessary and sufficient
for RNA t6A modification in vitro; null mutant lacks
N6-threonylcarbamoyl adenosine (t6A) modification in the
anticodon loop of ANN-decoding tRNA; member of conserved
YrdC/Sua5 family; binds single-stranded telomeric DNA and
null mutant has abnormal telomere length; GO_component:
GO:0005694 - chromosome [Evidence IEA]; GO_component:
GO:0000781 - chromosome, telomeric region [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IEA]; GO_function: GO:0003677 - DNA binding
[Evidence IEA]; GO_function: GO:0003725 - double-stranded
RNA binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016779
- nucleotidyltransferase activity [Evidence IEA];
GO_function: GO:0043047 - single-stranded telomeric DNA
binding [Evidence IDA] [PMID 19369944]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0006417 - regulation of translation
[Evidence IEA]; GO_process: GO:0006450 - regulation of
translational fidelity [Evidence IMP] [PMID 19884342];
GO_process: GO:0008033 - tRNA processing [Evidence IEA];
GO_process: GO:0000723 - telomere maintenance [Evidence
IMP] [PMID 19369944]; GO_process: GO:0070526 -
threonylcarbamoyladenosine biosynthetic process [Evidence
IDA] [PMID 23620299]; GO_process: GO:0070525 -
threonylcarbamoyladenosine metabolic process [Evidence
IMP] [PMID 19287007]; GO_process: GO:0070525 -
threonylcarbamoyladenosine metabolic process [Evidence
IMP] [PMID 21183954]"
/codon_start=1
/product="Sua5p"
/protein_id="XP_018736903.1"
/db_xref="GeneID:30033880"
/translation="MYTKVLPVDPESIRFDPDDINKPPSIASPSSTLDNLNEAVKILQ
TSHDPVAFPTETVYGLGASALDTESARAIYTAKNRPADNPLIIHVSSIEQLERRLLPA
GERVPEIYRGLINKFWPGPLTIILPVPDPTAISPVCTHGQSTFAVRLPANPVARALIS
LSDLPLAAPSANASTKPSPTQAQHVFTDLKGKIPLILDGGSSDIGVESTVVDGLSNPP
MLLRPGGVSIEQIREFGGPSWQNVVVGRATAKSDEVVRTPGMKYKHYSPRAPLTLFIG
CTEQQLIDYVIRSNPSSVGLLTTRHFNQKELAKTLRQLPGHITVHDKSLGQSGADISR
NLFASFREMDAKGVDLILVEGIDEQNEGLAIMNRARKAASTVISA"
gene complement(<138695..>141475)
/gene="PMR1"
/locus_tag="AWJ20_2015"
/db_xref="GeneID:30033881"
mRNA complement(<138695..>141475)
/gene="PMR1"
/locus_tag="AWJ20_2015"
/product="Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1"
/transcript_id="XM_018878941.1"
/db_xref="GeneID:30033881"
CDS complement(138695..141475)
/gene="PMR1"
/locus_tag="AWJ20_2015"
/inference="similar to AA sequence:KEGG_Orthology:K01537"
/note="High affinity Ca2+/Mn2+ P-type ATPase; required for
Ca2+ and Mn2+ transport into Golgi; involved in Ca2+
dependent protein sorting and processing; D53A mutant
(Mn2+ transporting) is rapamycin sensitive, Q783A mutant
(Ca2+ transporting) is rapamycin resistant; Mn2+ transport
into Golgi lumen appears to be required for rapamycin
sensitivity; mutations in human homolog ATP2C1 cause
acantholytic skin condition Hailey-Hailey disease;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IDA]
[PMID 24124599]; GO_component: GO:0000139 - Golgi membrane
[Evidence IDA] [PMID 9092527]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0005509 - calcium
ion binding [Evidence IDA] [PMID 10545175]; GO_function:
GO:0005388 - calcium-transporting ATPase activity
[Evidence IEA,IEA]; GO_function: GO:0005388 -
calcium-transporting ATPase activity [Evidence IDA,IMP]
[PMID 10545175]; GO_function: GO:0005388 -
calcium-transporting ATPase activity [Evidence IMP] [PMID
1379856]; GO_function: GO:0019829 - cation-transporting
ATPase activity [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0015410
- manganese-transporting ATPase activity [Evidence IDA]
[PMID 10801856]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_process:
GO:0070588 - calcium ion transmembrane transport [Evidence
IEA,IEA]; GO_process: GO:0006816 - calcium ion transport
[Evidence IEA,IEA]; GO_process: GO:0006816 - calcium ion
transport [Evidence IDA,IMP] [PMID 10545175]; GO_process:
GO:0006812 - cation transport [Evidence IEA]; GO_process:
GO:0006874 - cellular calcium ion homeostasis [Evidence
IGI] [PMID 23569283]; GO_process: GO:0006887 - exocytosis
[Evidence IGI] [PMID 1379856]; GO_process: GO:0006811 -
ion transport [Evidence IEA]; GO_process: GO:0006828 -
manganese ion transport [Evidence IDA] [PMID 10801856];
GO_process: GO:0008152 - metabolic process [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1"
/protein_id="XP_018736904.1"
/db_xref="GeneID:30033881"
/translation="MSEKSSNGRVETGPTPIANGQLPAATYCSLSSEETAARLHTSLT
VGLESSQDVNYRKSVHGPNELTGQEQDSLGIRFLQQFYENPLILLLIGSAAISFIMGN
IDDAVSITVAIFIVVTVGFVQEYRSEQSLEALNKLVPPSCHLTRGNHSSTVLASNLVP
GDLVHFSVGDRIPADVRITEAAGLSIDESNLTGEPHPVHKDIQEVKTESPLGTIPVSD
RTCIAFMGTLVCAGNGSGIVVGTGHNTEFGSVFEMMAEIDKPKTPLQISMDKLGKELS
LMSFGVIGMICLIGVIQGRSWLDMFQIGVSLAVAAIPEGLPIIVTVTLALGVLRMAEE
KAIMRRLPSVETLGSVNVICSDKTGTLTKNRMTVTKLWTVGMGSTSKSWLTIDSHSTV
NELTHHVTDNPALPVMLQVGNFCNNTRTSEETGKHIGNPTDVALIDVLDKFGLEDARN
TRTRVSETPFSSSRKWMAVSAHSGDATNSTVYVKGAVERILPLCKKYVDAKGKPNGTI
DEKSSKAINDVATTMALEGVRILAFAYSLKPSQDTENLQDLVFAGLIGMYDPPRPGVN
NSINRLMQGSVKVIMITGDSEATASTIAKQIGMPLNGERSVMTGETLERLSTKELAEA
ISYVTVFARTSPENKLNIVRALQARGDVVAMTGDGVNDAPALKLADIGISMGESGTDV
AKEAADMVLVDDDFNTILNAIKEGKAIFSNIQNFLAFQLSTSVAALTLVVLSTLFKLP
NPLNAMQILWINILMDGPPAQSLGVEPVDPVVMTKPPRSRNDHVLTRSLVQRVFQSGL
LIVFGTLFVYVREMSDNVVTARDTTMSFTCFVFFDMFNALSCRSTTKSVLEIGLFSNK
MFNFAVGFSLLGQMAVIYVPFFQAIFQTEALYLSDLILLIALSSSVLIVDEIRKHYVR
KNEWMSRYSNAV"
gene <140253..>140750
/locus_tag="AWJ20_2016"
/db_xref="GeneID:30033882"
mRNA <140253..>140750
/locus_tag="AWJ20_2016"
/product="unintegrated signal peptide"
/transcript_id="XM_018878942.1"
/db_xref="GeneID:30033882"
CDS 140253..140750
/locus_tag="AWJ20_2016"
/codon_start=1
/product="unintegrated signal peptide"
/protein_id="XP_018736905.1"
/db_xref="GeneID:30033882"
/translation="MTGSAGLSVTWCVSSLTVLWESIVSHDFDVEPMPTVHSLVTVMR
FLVKVPVLSLQITLTDPRVSTLGRRRIIAFSSAIRSTPSANVTVTIIGNPSGIAATAN
DTPIWNMSNHERPWITPIKQIIPMTPKLIKESSLPSLSMEICNGVLGLSISAIISNTL
PNSVL"
gene complement(<142679..>145846)
/gene="MET10"
/locus_tag="AWJ20_2017"
/db_xref="GeneID:30033883"
mRNA complement(<142679..>145846)
/gene="MET10"
/locus_tag="AWJ20_2017"
/product="sulfite reductase subunit alpha"
/transcript_id="XM_018878943.1"
/db_xref="GeneID:30033883"
CDS complement(142679..145846)
/gene="MET10"
/locus_tag="AWJ20_2017"
/inference="similar to AA sequence:KEGG_Orthology:K00380"
/note="Subunit alpha of assimilatory sulfite reductase;
complex converts sulfite into sulfide; GO_component:
GO:0009337 - sulfite reductase complex (NADPH) [Evidence
IDA] [PMID 6751400]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0016903 - oxidoreductase activity, acting on the
aldehyde or oxo group of donors [Evidence IEA];
GO_function: GO:0004783 - sulfite reductase (NADPH)
activity [Evidence IEA]; GO_function: GO:0004783 - sulfite
reductase (NADPH) activity [Evidence IDA] [PMID 6751400];
GO_process: GO:0008652 - cellular amino acid biosynthetic
process [Evidence IEA]; GO_process: GO:0019344 - cysteine
biosynthetic process [Evidence IEA]; GO_process:
GO:0070814 - hydrogen sulfide biosynthetic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0009086 - methionine
biosynthetic process [Evidence IEA]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]; GO_process: GO:0000103 - sulfate assimilation
[Evidence IMP] [PMID 19236486]; GO_process: GO:0000103 -
sulfate assimilation [Evidence IMP] [PMID 7928966]"
/codon_start=1
/product="sulfite reductase subunit alpha"
/protein_id="XP_018736906.1"
/db_xref="GeneID:30033883"
/translation="MSPSIDEKSDRVTNGQAKISGPFPISGDIMTLEGSFYASAQLLI
QQIAYSLSESIFSYSPEDFDLDSAVSEWSKFGQKSAKGITPQLHKLESRIGAASVLLG
YLYNRGGINSEVQTVLASSATLEYMKPVLAQFALEEDYKTPLVFQIAAVDYATETGKL
VTDYLPAAHISRDLGLGLISSLSVNDAQHIALLATALSRVLPTIHVYEGVRGVRETSR
TNNVLDAVDVSNKYDAIKASEGSLSSKILSTFENLNASLGTSYRPFEYSGDANAETVI
VSFGSVEGVIASQVVQALPNVGAVNVRLYSPFLDQEFLRLLPSSTKKIIVLGQVENQG
QVDDSRVHSGLYSDVATSVLMKYGFNGSAPTVADAKYSRETVWTPTEVFELLNSSPSD
LISESTKQFTFWDVDTSKSATAAATLAHTLSLDPTVAVNYFSAHDNETLGGVVESSIR
VSHQPLEAPYPVQKADVVFVNDVKILNEYNVLENARNGATLLISSKHAAEEVEKVLNN
AAKKSIAEKKVRLSTVDYEAIGENPETLGRTQTMVQQIAFWKTAYPQLTLNEITTKIV
TANGVDTELVAATVASLIEKVAEIGLSSASVPEEWAKVDQTVELSSSFVYPTSFTANK
LAGTIEQELNVVPESLTWVDAAKKVTFKEAYGVQSELRPDLSVKNFVSKVQVNERVTP
ADYDRHIFHLELDITGTGLTYAIGEALGVHAPNSTEQVNEFIEWYGLDPHEIVSIPAR
EDGDVYEEAKTTYHVLKDNLDIFGKPPKKFYESLATYATNPKEKSHLEKLASAEGAAE
LKKRAEEDFDTYADILHEFSSAHPTIAQLVEIVAPLKRREYSIASSQKVHPNAVHLLI
VVVDWVDSRGRKRFGQCSKFLSGLSAGDEVVVSVKPSVMKLPHDTKAPIIMSGLGTGL
APFKAFLEEKAWQKAQGYEIGDIYLFLGSRHQRQEYLYGELFEAYKNAGILTYIGAAF
SRDQPQKIYIQDRIKQAKEKLIDAFVTKGGSFYLCGPTWPVPDISGALADIVATEAAT
RGETIDAAREIENLKEQERYILEVY"
gene <148020..>150236
/gene="VID28"
/locus_tag="AWJ20_2018"
/db_xref="GeneID:30033884"
mRNA <148020..>150236
/gene="VID28"
/locus_tag="AWJ20_2018"
/product="glucose-induced degradation complex subunit
VID28"
/transcript_id="XM_018878944.1"
/db_xref="GeneID:30033884"
CDS 148020..150236
/gene="VID28"
/locus_tag="AWJ20_2018"
/note="GID Complex subunit, serves as adaptor for
regulatory subunit Vid24p; protein involved in
proteasome-dependent catabolite degradation of
fructose-1,6-bisphosphatase (FBPase); localized to the
nucleus and the cytoplasm; GO_component: GO:0034657 - GID
complex [Evidence IDA] [PMID 16872538]; GO_component:
GO:0034657 - GID complex [Evidence IDA] [PMID 18508925];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0030437 -
ascospore formation [Evidence IMP] [PMID 12586695];
GO_process: GO:0045721 - negative regulation of
gluconeogenesis [Evidence IMP] [PMID 12686616];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 12586695]; GO_process: GO:0043161 -
proteasome-mediated ubiquitin-dependent protein catabolic
process [Evidence IMP] [PMID 12686616]; GO_process:
GO:0043161 - proteasome-mediated ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 15358789];
GO_process: GO:0007039 - vacuolar protein catabolic
process [Evidence IMP] [PMID 15358789]"
/codon_start=1
/product="glucose-induced degradation complex subunit
VID28"
/protein_id="XP_018736907.1"
/db_xref="GeneID:30033884"
/translation="MIPTNSIRDQLRATDESTQLTGLKRLKNTIIGHTDQKELLCTDD
VINPLLSLLANGPLSVSLQASIVLGSFAHGSESTVQKLLRYPVIQTVITTINSVVCPD
VGTNEVLSPSPELISSCLRILVTILQAQPAVGVIAQYPEIIDILTILLSGDDCPSDIV
LQTCKLIPLLSPRKTASLTRLPSLAPYLLKRISNHMAQGGHRTQLVSRNSPLEAALSA
VSQILTEKQARSVLELTASNVISINSKAPAFSESSNDFLLGLVHLTRADDIGIRLTAV
ELLSKLQNYASSPVQRDIIARNLLPTLVPMFDLLGTDPRVSLTLSYICRDNEKHASLA
VEIGLVKKIITVLKAKDIASWKDREIIENNLYALAGIGIHRDSLRMEIVEAGALTYIT
NIISLKPAAESSSADISSIRKVKISACHVLRALSRSVALIRTSLASTDIVDGIVDLYT
ESEPSPELNDGIMSDESSFEVIEREQQREDQLEVRTAVMAAICNLILEFSPVQKTIFE
KGALDLIIDGAHSDYASLRLNSIWALKHAIFEANRDTKSMALSKLEPSYLLRLCNDSI
IEIQEQSLDFIRNFLCRNYQGVDYIFESIGKETLFELIEDKIDLHEQFPQVLTPAIYI
LVHIAAGTDEHRDIVAEQESVLSKLIPLMTHPLADVRTAIVWLIINLTWLEEPDNSPR
REVCKIRAERLARLGFKDMLDKLSQDPTLDVRERTKTALYQFDELFGRGSSNYVVS"
gene complement(<150344..>153412)
/locus_tag="AWJ20_2019"
/db_xref="GeneID:30033885"
mRNA complement(<150344..>153412)
/locus_tag="AWJ20_2019"
/product="hypothetical protein"
/transcript_id="XM_018878945.1"
/db_xref="GeneID:30033885"
CDS complement(150344..153412)
/locus_tag="AWJ20_2019"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736908.1"
/db_xref="GeneID:30033885"
/translation="MITLSNKFYREHDGSTAKNTKTPITTSLTIDQVLENESEVKLGG
KVKTKLHLFRLARLVKQIPTSPPSFTTHSRCAPASASTSNGSSVITSPQPDRVSNADN
ISSQTNHPPPSSLDDIQIPAVPCAPSTGINIYRSAPSPLPIFRSLKAGRNKEDRPTSS
DTKYYQQPNSYPGKFNRQQSDHTVQRSIDSVTCAKTAKHCSTKEELDVLGCSSHNRIG
ADDCIICTCTLVTSRSCGTTNPDHNVSRLDTQSNIVNNNKNTTFNSNNNSVNRNSDKD
KQQNPKTLLEFTERKSADTFPSSHPTLYNFPIKPTVDTSKVHGSPNSSVAVGNPIQTM
TSVGKGGFLLSSGPLKRGSRKGSGLGSGSGSTTKTASSTGFQVPTSSSNDVGSIGTGS
SGTTLPPSSPPQPSSPNAGSRPESKSLQRSKDTARMAALLMNLRYADETSNISDYGTT
LPTDTRVTGSPLTSNGKLMASSLAPSNSHVPKGAPLPLLDTSFGSSHMSHPGSSSPIS
NANLDSVTGSNHPPSPTVISSTTRARAIRAKARLELQYEMISEYQTGRRRNVMHFPEE
PDENKHDVESSDHVKTSENDNNVPSLGDRVKEKYTYNPLQVIRNRVAKKEQGNGYLHY
IVNNTAWNFYWHVDVSELVSDYAWRIRYGGNMRDREGKLLYPDRYKPDIRQDQDNDTS
GANTSGTGVSDPNNLSTSASKGEELIQRIKSKLSSRPPSGINSNENSDSSWSRSDISD
LEDNRANHRRENRQSTSSSRRNSESPTRKVPFESVNNKGRYSTSASQRKLSISNVPLA
VDETAADDSLLLEPTKNFAEADQSALTAKRVTPTSVSSTSLPSEQQQDKLAYYANELR
YLDMVYELSYLQISRKPYWYVENLNQDTAESMSTEMMALTKQFEGELIPKERQYLANT
EQEMRKLQFDLTQDYGSRLDSVLLDSDRILNEVNTSLSLEIRKVGMRLDKLEANAPTL
QWMWKLAYMLLEWLLVAFMWLAWGVVVGMRQLRRLVALVYGIVKWLLWCS"
gene <154317..>154637
/gene="TRS20"
/locus_tag="AWJ20_2020"
/db_xref="GeneID:30033887"
mRNA <154317..>154637
/gene="TRS20"
/locus_tag="AWJ20_2020"
/product="Trs20p"
/transcript_id="XM_018878947.1"
/db_xref="GeneID:30033887"
CDS 154317..154637
/gene="TRS20"
/locus_tag="AWJ20_2020"
/note="Core component of transport protein particle
(TRAPP) complexes I-III; TRAPP complexes are related
multimeric guanine nucleotide-exchange factors for the
GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI),
intra-Golgi traffic (TRAPPII), endosome-Golgi traffic
(TRAPPII and III) and autophagy (TRAPPIII); mutation of
conserved residues leads to defects in endocytic recycling
and a block in sporulation/meiosis; mutations in the human
homolog cause the spondyloepiphyseal dysplasia;
GO_component: GO:0005794 - Golgi apparatus [Evidence
IEA,IEA]; GO_component: GO:1990070 - TRAPPI protein
complex [Evidence IDA] [PMID 11239471]; GO_component:
GO:1990071 - TRAPPII protein complex [Evidence IDA] [PMID
11239471]; GO_component: GO:1990072 - TRAPPIII protein
complex [Evidence IDA] [PMID 20375281]; GO_component:
GO:0005783 - endoplasmic reticulum [Evidence IEA,IEA];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0000407 - pre-autophagosomal structure
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0006888 -
ER to Golgi vesicle-mediated transport [Evidence IEA];
GO_process: GO:0006888 - ER to Golgi vesicle-mediated
transport [Evidence IMP] [PMID 11239471]; GO_process:
GO:0006888 - ER to Golgi vesicle-mediated transport
[Evidence IGI] [PMID 9564032]; GO_process: GO:0006914 -
autophagy [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA,IEA]; GO_process: GO:0016192 -
vesicle-mediated transport [Evidence IEA]"
/codon_start=1
/product="Trs20p"
/protein_id="XP_018736909.1"
/db_xref="GeneID:30033887"
/translation="MASYYLAIIGTKDCPIYELEFGSYRQSGDGISKFPPEIKELSPF
ILHSSLDIVEDVQWTTNSLYLKSVDNFYSYMVSAFVTPGSKYTISGANLGVGPKFRLE
EPAD"
gene complement(<155042..>156616)
/locus_tag="AWJ20_2021"
/db_xref="GeneID:30033888"
mRNA complement(<155042..>156616)
/locus_tag="AWJ20_2021"
/product="hypothetical protein"
/transcript_id="XM_018878948.1"
/db_xref="GeneID:30033888"
CDS complement(155042..156616)
/locus_tag="AWJ20_2021"
/inference="similar to AA sequence:KEGG_Orthology:K11560"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736910.1"
/db_xref="GeneID:30033888"
/translation="MARPRKSDAGPNGSDVSGQPVPHTTSSNVEVTNPPKKKRGRPRK
SVSREEDDGFVFKRLDSESSLNSSTAKPVSSSKKVTTLKETVHLALEQAKSQSISSSR
SEPEKAKKIASKSSSSQKHSAHSSKENEKQKKRSQREHDHAHSKDSDKRQKLSKMRPR
DTTSGSAIATPPPSIPSSDNHDSQPGSYDDSIAGRTSTTQVLLHDQETPVIRRNQKLR
NQTTTGKRRSSLSNRGKRLSNIGNGYLADPHSDVDARDFSKHLDENLSDVQRLKQVLL
WCLKRNLEVHGEDLKRKRDQSTKSTSEELTALTIARQIFEEEVVKNLVDGKISIDWHS
RPENAGQANVKRIPNSLNVTAQNNLTAFQKRLEELEREKEELERNLQSVGKSRLSKER
LDNLIDDNILNTLDDQTRRLFLEYKSSSTPVLEPSIQLRSFRENLPNVEVGVTQLRDG
VHKIESITKAASIFIDKTMKKLSEAAAITYANNNSTQLVLTIPEANSDADRSESSLRT
PVSPHDILRAISRLES"
gene <158301..>159746
/locus_tag="AWJ20_2022"
/db_xref="GeneID:30033889"
mRNA <158301..>159746
/locus_tag="AWJ20_2022"
/product="Mitochondrial distribution and morphology
protein 34"
/transcript_id="XM_018878949.1"
/db_xref="GeneID:30033889"
CDS 158301..159746
/locus_tag="AWJ20_2022"
/inference="similar to AA sequence:KEGG_Orthology:K17775"
/codon_start=1
/product="Mitochondrial distribution and morphology
protein 34"
/protein_id="XP_018736911.1"
/db_xref="GeneID:30033889"
/translation="MSFKFNWSFFNKDSLLDQTSEVFKVAMNKGIKPDIIIGDMAVDK
FELGTVPPSLEILEIGDLAEDKFRGIFKLEYAGDASLSIKTQVQANPLALLNAQSCRL
RGAFPKIVGAACPLELPLSIFISEFKLSAIVIIVFSMARGLTLVFQNDPLESVKVSST
FDSLPGIAEFLQQEVEARLRESLRDDIPEVLHKLSQEKLVSSNRSEGMKELMTHLQHT
PHSHRYSNAFAFTNDSSPIPDLFHGSDETVNGLHDLDASHSTLSIRADSMIPDCVTRS
TLASFEEDLKAKAAMEEDAQRLKYQARHKGSRRYSSTSSSTSLSELGAMSPIMDAYPR
LYRDVRPKKRVIKLSKTNSTISEPSTISPSTQLESPNITASTSTHCTPASPQSPPSRS
TPEMSPLHSNKGSPRERGRPKRRELSFASIRSPSPIDLDFRAVLEKQKQKENLSFTLN
GASEKTLSKWAINNQHQIFAPPKYEELREDL"
gene complement(<162624..>165347)
/gene="SSM4"
/locus_tag="AWJ20_2023"
/db_xref="GeneID:30033890"
mRNA complement(<162624..>165347)
/gene="SSM4"
/locus_tag="AWJ20_2023"
/product="E3 ubiquitin-protein ligase SSM4"
/transcript_id="XM_018878950.1"
/db_xref="GeneID:30033890"
CDS complement(162624..165347)
/gene="SSM4"
/locus_tag="AWJ20_2023"
/inference="similar to AA sequence:KEGG_Orthology:K10661"
/note="Ubiquitin-protein ligase involved in ER-associated
protein degradation; located in the ER/nuclear envelope;
ssm4 mutation suppresses mRNA instability caused by an
rna14 mutation; GO_component: GO:0000837 - Doa10p
ubiquitin ligase complex [Evidence IDA] [PMID 16873066];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0030176 -
integral component of endoplasmic reticulum membrane
[Evidence IDA] [PMID 11641273]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005635 -
nuclear envelope [Evidence IDA] [PMID 11641273];
GO_component: GO:0005637 - nuclear inner membrane
[Evidence IEA]; GO_component: GO:0005637 - nuclear inner
membrane [Evidence IDA] [PMID 17051211]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_function:
GO:0016874 - ligase activity [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0004842 - ubiquitin-protein transferase
activity [Evidence IDA] [PMID 11641273]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0030433 - ER-associated ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 11641273];
GO_process: GO:0030433 - ER-associated ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 15252059];
GO_process: GO:0016567 - protein ubiquitination [Evidence
IEA]"
/codon_start=1
/product="E3 ubiquitin-protein ligase SSM4"
/protein_id="XP_018736912.1"
/db_xref="GeneID:30033890"
/translation="MIVREWILLNDAFVRDLQMAGNAPVGNIANAIHNNNAQMVPQLR
IPNPRIEAVRAVDENPLDIELDRLRADFGAFDAPQAERANGENVALEGRDQNVENANA
IANDNANGRDGRIHRPQDFALPVNVREDDFFFGGVDDEPEDLGEVGEVEGIRDIIGLK
GPIVNAFATCAAATVLSAIALACLYLLPYATGRLVVILLGESVSPILKFYKQTLESQV
TWVNNIFFVFASFIDTRILASNPDFSLVKFHEAVKDLTFNLINGIPSSVNSSIMIRAS
ITSRLLLTFLGYSISGTFLAAFISNSNLTASRTGRDMLRVLKEMGAICKVITVIGIEL
VLFPIFCGLLLDFSLLPLFETASIADQVNFAVKYPVISTILHWALGTCYMFIFAMFVS
LCRKIMRPGVLYFVRDPNDPNFHPIKDVLDRGLMSQLAKIGLSALIYGALIVLCLGGV
IYTLRYLNVEFLPVSFVSTTREHIVIMIPFCAAYFLTLPIFIRTVRNLSVMRKIWAYA
FESSCSYLRLSSFILNKPVPQEQGTVHYGSIAAWFMSSKPDYSRPVTIDDIAKNGKWD
GAYFVKDGGFVRAPDTDSISSKKKLNLFIPVTKDDVRLDGVEEGSESNELEKYSVVYR
PPHFRIRIALLLCCIWGYGALVVMSMILIPIYTGHVMSQFLLHPIFKDMCDVFQFILG
SIPLFILFYTADNWDRVKEKFLNEYNQLRQSPLASSEAASGIVHLFKLLFSILVCLVV
PTAIGGILDLYISSPLYHVLSSEIVYSKSMLTKFISGVLSILLAKNVVMQHAPNSSLA
RSFRALSENGWIGYQNPMAATKLLLPLMSILGLAAICPPFLCWALLKIWYHDVSPETQ
ARMYVFCYPVLVVWIALDLAIYSVRTAIKLWVTKARDDIYLVSRQLENLEH"
gene complement(<165903..>166142)
/gene="SSM4"
/locus_tag="AWJ20_2024"
/db_xref="GeneID:30033891"
mRNA complement(<165903..>166142)
/gene="SSM4"
/locus_tag="AWJ20_2024"
/product="E3 ubiquitin-protein ligase SSM4"
/transcript_id="XM_018878951.1"
/db_xref="GeneID:30033891"
CDS complement(165903..166142)
/gene="SSM4"
/locus_tag="AWJ20_2024"
/inference="similar to AA sequence:KEGG_Orthology:K10661"
/note="Ubiquitin-protein ligase involved in ER-associated
protein degradation; located in the ER/nuclear envelope;
ssm4 mutation suppresses mRNA instability caused by an
rna14 mutation; GO_component: GO:0000837 - Doa10p
ubiquitin ligase complex [Evidence IDA] [PMID 16873066];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0030176 -
integral component of endoplasmic reticulum membrane
[Evidence IDA] [PMID 11641273]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005635 -
nuclear envelope [Evidence IDA] [PMID 11641273];
GO_component: GO:0005637 - nuclear inner membrane
[Evidence IEA]; GO_component: GO:0005637 - nuclear inner
membrane [Evidence IDA] [PMID 17051211]; GO_component:
GO:0005634 - nucleus [Evidence IEA]; GO_function:
GO:0016874 - ligase activity [Evidence IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0004842 - ubiquitin-protein transferase
activity [Evidence IDA] [PMID 11641273]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0030433 - ER-associated ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 11641273];
GO_process: GO:0030433 - ER-associated ubiquitin-dependent
protein catabolic process [Evidence IMP] [PMID 15252059];
GO_process: GO:0016567 - protein ubiquitination [Evidence
IEA]"
/codon_start=1
/product="E3 ubiquitin-protein ligase SSM4"
/protein_id="XP_018736913.1"
/db_xref="GeneID:30033891"
/translation="MDLTSPTKDQESPGYSDHGEPSSDTNSQSSLPQSSSINEHVEPP
DDDTCRICRGEASEEEPLYHPCKCSGSIKYVHQDW"
gene <167427..>169031
/gene="TRM2"
/locus_tag="AWJ20_2025"
/db_xref="GeneID:30033892"
mRNA <167427..>169031
/gene="TRM2"
/locus_tag="AWJ20_2025"
/product="Trm2p"
/transcript_id="XM_018878952.1"
/db_xref="GeneID:30033892"
CDS 167427..169031
/gene="TRM2"
/locus_tag="AWJ20_2025"
/inference="similar to AA sequence:KEGG_Orthology:K15331"
/note="tRNA methyltransferase; 5-methylates the uridine
residue at position 54 of tRNAs and may also have a role
in tRNA stabilization or maturation; endo-exonuclease with
a role in DNA repair; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0008173
- RNA methyltransferase activity [Evidence IEA];
GO_function: GO:0030697 - S-adenosylmethionine-dependent
tRNA (m5U54) methyltransferase activity [Evidence
IEA,IEA]; GO_function: GO:0051908 - double-stranded DNA
5'-3' exodeoxyribonuclease activity [Evidence IDA] [PMID
17205207]; GO_function: GO:0008168 - methyltransferase
activity [Evidence IEA]; GO_function: GO:0000014 -
single-stranded DNA endodeoxyribonuclease activity
[Evidence IDA] [PMID 17205207]; GO_function: GO:0030696 -
tRNA (m5U54) methyltransferase activity [Evidence IDA,IMP]
[PMID 10864043]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0006281 - DNA
repair [Evidence IGI,IMP] [PMID 17205207]; GO_process:
GO:0001510 - RNA methylation [Evidence IEA]; GO_process:
GO:0006396 - RNA processing [Evidence IEA]; GO_process:
GO:0006302 - double-strand break repair [Evidence IGI,IMP]
[PMID 17534700]; GO_process: GO:0032259 - methylation
[Evidence IEA]; GO_process: GO:0006400 - tRNA modification
[Evidence IDA] [PMID 10864043]; GO_process: GO:0008033 -
tRNA processing [Evidence IEA,IEA]"
/codon_start=1
/product="Trm2p"
/protein_id="XP_018736914.1"
/db_xref="GeneID:30033892"
/translation="MTDTGLIQANSKVTAEPVVNNDKIHPRSPSLDPTPLKKQKRVKT
KRYREPPVDPISAEGVLINDIRALLKSQGISEDEITNDMKAFFSRDKSVPYPDYQVVE
LKISALSSVGNGIGIWESPDPEAPSSIRKRVVVVPYALVGETVKTKIYRTNKYYYESE
LNEVVDKSPLRDDALVRCKYFTTCSGCQYQMLPYDEQLKIKREVIVNAYAHYAPLLTS
DDVPSILDTVGSPEQYNYRTKLTPHFDVPRKGRLERIPPIGFGVKGRKEVLDIEECVI
GTPVINKGMVEQRKVVHENFASYKRGATILLRENIKEIEDSPSTVVCTTDSKEIVTEY
VGDFKFKYPAGSFFQNNNSILPSVTSYVRDNIVLPSSGKPPKYLVDTYCGSGLFGITC
STAVEQLIGVEISKDSVEYAQKNAELNNLKNASFIVGEAEKIFDKVSDAPLETSIIID
PPRKGCDKQFLDQLLEFRPAKVVYVSCNVHSQARDIEYLLKSEVARDRSSNSGNKYII
DSIRGFDFFPQTHHVESVAVLSLKNN"
gene complement(<175785..>177227)
/locus_tag="AWJ20_2027"
/db_xref="GeneID:30033893"
mRNA complement(<175785..>177227)
/locus_tag="AWJ20_2027"
/product="hypothetical protein"
/transcript_id="XM_018878953.1"
/db_xref="GeneID:30033893"
CDS complement(175785..177227)
/locus_tag="AWJ20_2027"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736915.1"
/db_xref="GeneID:30033893"
/translation="MFLKYDPDTDLLKLDLEQIPVFLHIRLAQLVLESLNCFIVVARY
GSEELKSQMYQLDPNLVLEWRTNFAEILELRKAVQVYEEKSNAFGLSRVLWNKQSDLT
PRSVDVDTVNFFDVPSQYPSIPSINASLDRADRSHKGDIVEKIVDGTLFGTLREARDD
ELPIEMVAQLSFAPIIRTQWKLANKLVMRIFKGEQDIVDHARLIEQTYFLANGELAIR
LEERIFGTTTQGGLALDAQHFGMEISGSWQPTSLEINSCLSGVLEDVIRKLPGVCNEV
RQAGFINFGFSEKNRQQSNLFATSVLKLTYRVPKPLSLIFNSKAIYYLDKIFQRLIMG
LRLTHWLKMKRHGVYCVQNQQLRNFISCSLSSINLAIRNIWEALINKLVVMNVEEETS
LEQVISEVTTALDRIFTNMISGPEEELLFSIYSVVFEPTTDDDKLRKLIVQLISQLQK
QTIQANTDALIFTRIFTDQLQFSGFYERND"
gene complement(<178693..>178766)
/locus_tag="AWJ20_2028"
/db_xref="GeneID:30033894"
tRNA complement(<178693..>178766)
/locus_tag="AWJ20_2028"
/product="tRNA-Ile"
/db_xref="GeneID:30033894"
gene complement(<178914..>179924)
/locus_tag="AWJ20_2029"
/db_xref="GeneID:30033895"
mRNA complement(<178914..>179924)
/locus_tag="AWJ20_2029"
/product="Bifunctional purine biosynthesis protein purH"
/transcript_id="XM_018878954.1"
/db_xref="GeneID:30033895"
CDS complement(178914..179924)
/locus_tag="AWJ20_2029"
/codon_start=1
/product="Bifunctional purine biosynthesis protein purH"
/protein_id="XP_018736916.1"
/db_xref="GeneID:30033895"
/translation="MPKPIRLTASKSSRINKPSVGKKPALPLMPSDIAFLKDFDEKKS
LRQARSKAQQEKLQDDGPAELEWQLQVSTIPDIVKSMRIVLDRMWAPIPEKGLGREGR
GDVYHFRKSIPPLVRTTHLYAIYPDNSTYVDREIRTACEDGTVRRLVVNLVEGSDLLI
ESTHYYAILDEQVRKYEESLSKSEDEGSKVVTSAIVKALKIFKKLLIKNPTAISLSLS
ELEDVDLAKLRNNLLSLGFLTLQSGQMDSFVISVPNIGSYLRLVASSRKWIIRNLDKM
SWKELLESTLQERWESNKTYWYDFKGCLLEWVLYDCFGGGWCEPFKTPVGRGWKLTGK
KG"
gene <180932..>183247
/locus_tag="AWJ20_2030"
/db_xref="GeneID:30033897"
mRNA <180932..>183247
/locus_tag="AWJ20_2030"
/product="hypothetical protein"
/transcript_id="XM_018878956.1"
/db_xref="GeneID:30033897"
CDS 180932..183247
/locus_tag="AWJ20_2030"
/inference="similar to AA sequence:KEGG_Orthology:K01127"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736917.1"
/db_xref="GeneID:30033897"
/translation="MEDWIHEFEKADKQDKGQVNIGPRSIDSVVRNLKMNAFQRVLNA
FKYKERGRVHNSGAKDESDNELALKAFIFGILTHQVADISWHSLGIQQGLLRMLANRE
FNGDVAEAHSVLDTGGDMIYVRRMLQSGLNLDWIQGEWKYPTADILEIISRAGYSVTS
SQLDYCMFRGKAALGAELQLAVSAFAPYAQRSPLLFEHIDDYFLGGVTEMSTATRHCS
YSLAEWFANQTTPADPWSVCEVYEGMKGKSYRNSNFLYENETLPTEDYTTQSTPYLDI
PLAQSISSDTKLEISAHQNIFGDSDSKVLAELATVISTGVPNGAFGTSLTIGKFLNDE
LCLAISAPYESVTFGFGRTAKEVPKGSVYVVELSQLVVSNNNIAVKTPAKMITLEMPE
EGPPYQFNERFGAKMTKITLAGFDVLIVSRPGTSRLSFFLENRNIFNIHWGSASVKYG
SFGDKLAGESLVVGDVDGDGIDDLVVGSPYSDISGRPQAGEVHVINGLSLQSIFSVAV
ESRSRVPLEFDIKDVAEIVLHLPSLTSSPGYSLFGSKVAVVSRGSTNYLLIGAEGVGK
VFAFDSKKDFKYVFVLSSNVNTGANFGGKLLVGNNDGWIAVGSTTEDVHSDRTGRVKC
SQCGAIYLFRIKEIGVIEDLSLYNSTDELTAYEQGYLGFKTRKGMESGSFSRFGHEGT
IIDKYLYTSSPFGQNERGQVWRLDLSKHGKGNFELLIEESPWPYSNGFGTSLAGIQQN
GETFLASGMPYYGTNERGWLQGAVALYHLKD"
gene <186176..>188560
/gene="SUL1"
/locus_tag="AWJ20_2031"
/db_xref="GeneID:30033898"
mRNA <186176..>188560
/gene="SUL1"
/locus_tag="AWJ20_2031"
/product="Sul1p"
/transcript_id="XM_018878957.1"
/db_xref="GeneID:30033898"
CDS 186176..188560
/gene="SUL1"
/locus_tag="AWJ20_2031"
/inference="similar to AA sequence:KEGG_Orthology:K14708"
/note="High affinity sulfate permease of the SulP anion
transporter family; sulfate uptake is mediated by specific
sulfate transporters Sul1p and Sul2p, which control the
concentration of endogenous activated sulfate
intermediates; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA,IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA,IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence IMP,ISS] [PMID 7616962]; GO_function:
GO:0008271 - secondary active sulfate transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0015116 - sulfate transmembrane transporter activity
[Evidence IEA]; GO_function: GO:0015116 - sulfate
transmembrane transporter activity [Evidence IGI,IMP]
[PMID 7616962]; GO_process: GO:1902358 - sulfate
transmembrane transport [Evidence IEA]; GO_process:
GO:0008272 - sulfate transport [Evidence IEA]; GO_process:
GO:0008272 - sulfate transport [Evidence IGI,IMP] [PMID
7616962]; GO_process: GO:0055085 - transmembrane transport
[Evidence IEA]; GO_process: GO:0055085 - transmembrane
transport [Evidence IMP] [PMID 7616962]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Sul1p"
/protein_id="XP_018736918.1"
/db_xref="GeneID:30033898"
/translation="MSTGSDHLFSSSGNGANDEAEPSYISIPQYDEPPVSIKDFGREV
FADTPKKIKEYLLSLFPIIRWIYRYNFVWLYGDLIAGITVGAVLVPQSMSYAKIATLP
PQIGLYSSFVGVMIYCFFATSKDVSIGPVAVMSLQTSKAIAHVIHKHPEYANDPTYIA
LGLSLLCGAIALGIGLLRLGFILEYIPMPAVLGFMTGSAFTIAAGQVPGLMGISKLFN
TRDATHLVVIHTLQNLKHSNLDAAFGLVSLFILYFSRWLFAFLGKRYPKYERVFFFGS
VLRNAFVIIFSTLISWGICHHHKNPKKFPISVLGTVPRGLQNVGYHTIDQNLASALAP
ELPISTVILLMEHIAIAKSFGRVNDYRINPNQELIAIGTTNMIGTFFNAYPATGSFSR
SALKSKCGVRTPLAGIFTGIVVIVAIYGLTGTFYWISNASLSAVIIHAVTDLMAPWYV
TWKFWKSSPVETIVFLGAVFLSVFVSLESGIYFSFAACLVFLLLRISFPTGQFLGRVE
VVEYLNPNYSEANGAIEETSRIDNDPEDKKKYEVVRQTSSSGSANPKPTTVKKYTWVP
LNHHNINKDVLIQPPPPGVFVFRPGESFTFPNAARQLEILVQEVRKQTRKGGVQQHQL
TLGERPWNDHGPRHKVYDEGFVDERPLLRAVVFDFSASPHIDLNGVQALADIRQELNK
YANREVEYHFVGILSVWSRRALMAHSFGGDNSETRPERHYVEISLRQAAGVDGSATEA
VEEESKKKNKYRDEEERDTVSQLSNDGSYIPIIATNTPFFHLEIPNLEFPESRE"
gene complement(<188760..>189698)
/gene="YJU2"
/locus_tag="AWJ20_2032"
/db_xref="GeneID:30033899"
mRNA complement(<188760..>189698)
/gene="YJU2"
/locus_tag="AWJ20_2032"
/product="Yju2p"
/transcript_id="XM_018878958.1"
/db_xref="GeneID:30033899"
CDS complement(188760..189698)
/gene="YJU2"
/locus_tag="AWJ20_2032"
/note="Essential protein required for pre-mRNA splicing;
associates transiently with the spliceosomal NTC
('nineteen complex') and acts after Prp2p to promote the
first catalytic reaction of splicing; GO_component:
GO:0071006 - U2-type catalytic step 1 spliceosome
[Evidence IDA] [PMID 17515604]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14562095]; GO_function:
GO:0000384 - first spliceosomal transesterification
activity [Evidence IDA] [PMID 17515604]; GO_process:
GO:0000349 - generation of catalytic spliceosome for first
transesterification step [Evidence IDA] [PMID 17515604]"
/codon_start=1
/product="Yju2p"
/protein_id="XP_018736919.1"
/db_xref="GeneID:30033899"
/translation="MGDRKTVNKYYPPDFDPSKITKKRKSIKESAAASLQTVRLMAPF
SMRCTTCGEFIYRGKKFNAKKQPTGESYLGIKIIRFYIRCPRCAGEIRFKTDPKIGDY
VTEQGAERNREPWREKAEEEEDLEQRLDRLEREEIEQQEREQLRKQYGIAGAVTAKKD
QNNPDADIMAELESKVLDSKREMEIQDELDAIRTRNARIEMAASGGGLLERAQEVVQS
AVVGKKKLEEEEDEEAARKAFKRTSDGVIIRQARPPDDTGPSATSTSNVSSTVPIKPT
VPNASLFNPTSVLKKPVKGGKKLNALGVVVKKKSLV"
gene <190962..>193301
/gene="TRK2"
/locus_tag="AWJ20_2033"
/db_xref="GeneID:30033900"
mRNA <190962..>193301
/gene="TRK2"
/locus_tag="AWJ20_2033"
/product="Trk2p"
/transcript_id="XM_018878959.1"
/db_xref="GeneID:30033900"
CDS 190962..193301
/gene="TRK2"
/locus_tag="AWJ20_2033"
/note="Component of the Trk1p-Trk2p potassium transport
system; contributes to K(+) supply and maintenance of
plasma-membrane potential; TRK2 has a paralog, TRK1, that
arose from the whole genome duplication; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA,IEA]; GO_component:
GO:0005886 - plasma membrane [Evidence TAS] [PMID
2072919]; GO_function: GO:0008324 - cation transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0015079 - potassium ion transmembrane transporter
activity [Evidence IEA]; GO_function: GO:0015079 -
potassium ion transmembrane transporter activity [Evidence
IDA,ISS] [PMID 2072919]; GO_process: GO:0006812 - cation
transport [Evidence IEA]; GO_process: GO:0030007 -
cellular potassium ion homeostasis [Evidence IDA] [PMID
2072919]; GO_process: GO:0006811 - ion transport [Evidence
IEA]; GO_process: GO:0071805 - potassium ion transmembrane
transport [Evidence IEA]; GO_process: GO:0006813 -
potassium ion transport [Evidence IEA]; GO_process:
GO:0042391 - regulation of membrane potential [Evidence
IMP] [PMID 21424545]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Trk2p"
/protein_id="XP_018736920.1"
/db_xref="GeneID:30033900"
/translation="MTFLCSIILYPGGNIRYIDALFIGAAGCTQAGLNTVQLNELYTY
QQIFLMIFPFLTTPIFIHSSVVFLRLHWFEKHFKDVKINSKIQSKLRRTATMARAESR
DHEIGLEPLKWNGEYSKRDSNPPVPTSNNTEEVSGLHVPPASDIRFGDLPQPRRQKSI
APQDMYKSINMMQRNRRLSNDLNDGPALVIKSPRVMDEENELKEKMRLAATVTGQNVD
SSPSSSGNASTEHESVDLGERIRPSISYAVSPERSNYDIDGIKLDRRSRTLSTSNVPN
ENQRSQHERTGSKASVTRGTRRFSRSRTLDRIFNRTLSNDGPTTKVMSTNYLSWQPTV
GRNSAFVDLTEEQKEELGGVEYRSLKLLAKVLVAYYMGFYLFGLVTFWPYASLRNLLK
DHFTSEGFTSTLWAWFTSVSAFSDTGFTLTPESMAFFQREAYIPLLSAFLIVIGNTGF
PVLLRFIIWIAFKFTPRFGRAHESLGFLLDHPRRCFTLLFPSSATWWLLAVLVILNSV
DLVLFLVLDLTSKAITEIPVGYRIVCGLFQAIATRTAGFTIVSMSDVHPAVRVSYVIM
MYISVLPIAISVRRTNVYEEQSLGVYYPENDGEDPEAKHHSYVGAHLKKQLYFDLWFI
CLGLFIICIAEGAKLDNNEFQIFDVLFEIVSAYGTVGLSMGAANTNLALSGAFNVIGK
LVIIALMIRGRHRGLPYALDRAIILQGDKIQRTDSIQETRTRNKRLMSMSIPINDDVG
LTTARSNDTGYMRERPGLRTETSTLDRFPSQAVSDGEDD"
gene <194218..>195855
/gene="TPO1"
/locus_tag="AWJ20_2034"
/db_xref="GeneID:30033901"
mRNA <194218..>195855
/gene="TPO1"
/locus_tag="AWJ20_2034"
/product="Tpo1p"
/transcript_id="XM_018878960.1"
/db_xref="GeneID:30033901"
CDS 194218..195855
/gene="TPO1"
/locus_tag="AWJ20_2034"
/note="Polyamine transporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; recognizes spermine, putrescine, and
spermidine; catalyzes uptake of polyamines at alkaline pH
and excretion at acidic pH; during oxidative stress
exports spermine, spermidine from the cell, which controls
timing of expression of stress-responsive genes;
phosphorylation enhances activity and sorting to the
plasma membrane; GO_component: GO:0033101 - cellular bud
membrane [Evidence IDA] [PMID 13679573]; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IMP]
[PMID 11171066]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA,IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence IEA,IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence IDA] [PMID 12562762]; GO_component:
GO:0005886 - plasma membrane [Evidence IDA,IMP] [PMID
15637075]; GO_function: GO:0015297 - antiporter activity
[Evidence IEA]; GO_function: GO:0015606 - spermidine
transmembrane transporter activity [Evidence IMP] [PMID
11171066]; GO_function: GO:0015606 - spermidine
transmembrane transporter activity [Evidence IDA,IMP]
[PMID 15637075]; GO_function: GO:0000297 - spermine
transmembrane transporter activity [Evidence IMP] [PMID
11171066]; GO_function: GO:0000297 - spermine
transmembrane transporter activity [Evidence IDA,IMP]
[PMID 15637075]; GO_function: GO:0000297 - spermine
transmembrane transporter activity [Evidence IMP] [PMID
9920864]; GO_process: GO:1902047 - polyamine transmembrane
transport [Evidence IMP] [PMID 24136413]; GO_process:
GO:0015847 - putrescine transport [Evidence IMP] [PMID
9920864]; GO_process: GO:0015848 - spermidine transport
[Evidence IMP] [PMID 11171066]; GO_process: GO:0015848 -
spermidine transport [Evidence IDA,IMP] [PMID 15637075];
GO_process: GO:0015848 - spermidine transport [Evidence
IMP] [PMID 9920864]; GO_process: GO:0000296 - spermine
transport [Evidence IMP] [PMID 11171066]; GO_process:
GO:0000296 - spermine transport [Evidence IDA,IMP] [PMID
15637075]; GO_process: GO:0000296 - spermine transport
[Evidence IMP] [PMID 9920864]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Tpo1p"
/protein_id="XP_018736921.1"
/db_xref="GeneID:30033901"
/translation="MSSGNSADASGIGDSSPNQSSRNSEEFPLDVEKVISVNGGFKKN
GEEQDPSPGPSHKQENADTDSNENTDLLDPRQYDENIFLVTFSKGDKENPQNWSLRKK
SLHTAFYGLTTFAAQFNSAVMSPTVPHLMDSFGISREVAILGTSLYVLGIAFGPSVFA
PYSEMNGRKMGVMLPFFISLIFTAGTASSDNISAILCTRFFAGFFAGAPVVSSGGVLA
DIWDPAIRGVALIFYAVFVTAGVTFGSILSSVVTSGSDTDWRWSCWLIVIIGGVILFF
DVILLSETYAPVILAKRASNLRINTHQWAYHAKHDEWKLDLKEFLTLHLLRPFAMFAT
PIVFCIAMFASFVFGVLYLVITSVPITFQITKGWEGTVATLPMVALFLGLITGSGLNV
WSGLRYAKLVRANNNKPLPEQRFPVMMMFGWLMPAGTFIFAWTLRPEIHWIVPMIGIY
LLGTGFIVIFQGCLNYLVDTFTRFAASAIAANTFVRSIFGGIFPLFGDTMFINLGVNW
GGSLVGFIALALTPIPYIFYLYGEKLRSRNPYSKLVS"
gene <197062..>199857
/gene="PBS2"
/locus_tag="AWJ20_2035"
/db_xref="GeneID:30033902"
mRNA <197062..>199857
/gene="PBS2"
/locus_tag="AWJ20_2035"
/product="mitogen-activated protein kinase kinase PBS2"
/transcript_id="XM_018878961.1"
/db_xref="GeneID:30033902"
CDS 197062..199857
/gene="PBS2"
/locus_tag="AWJ20_2035"
/inference="similar to AA sequence:KEGG_Orthology:K11227"
/codon_start=1
/product="mitogen-activated protein kinase kinase PBS2"
/protein_id="XP_018736922.1"
/db_xref="GeneID:30033902"
/translation="MSVYHGTSNNDYLAVDRRESSSSSPMPPTPRPSISDQGCGSSVN
NDEGLTVSSNAGSSSVPLPLPSPGIVFSKQEDFEEYQTDSRQAQAYHNGVSANVALPR
TSSQAKPRQSQASANSLSRHTYNETYSSSMNSLNSFTSSTSNHLPPRVSSYNNSSTNI
SGLTRSTSSSAGSSSSSTPKPMLNSRSTSTGARFQRVNRQASHGNTISPDETIAEALS
AKLKSNLALSTSPSASPPPVSVASSPSLSTLSPSSANGTNSPALGSTPPTQWAASAIM
NGGIASVSPDVHARILTFQQSRQSRSNSGSNAALNFIPGSVPLLPNDGAPAGSPSSPN
LVGLTGGNMCTPQAIKDNLSRSSSSSSSSSYSNYSRTISSYSSPIVSPHPEDKGSEKL
PPARQLAQPLQLSQKTSNLQIKIPSTIEDSDGTDSGDDHGTANPTIESAATIVPSADS
SLNTPQSATQTGKPFSGALFINSVSQGTKIPTQRPSLSERRGLKLSDMSQPPPIPKLK
SHPLLPSTGTGRSTSLLDRRMQASAPAGLFPKWSGKLNMDPSSERATPNISGGEGDQA
NGADSGSGPGAQAPVVLRPPGVGGFNRQNSLFSNYKKYVDVKSGSLNFAGKASLHSKG
VDFSSGSSFRISIDEFEPMGELGRGNYGTVTKVLHKPTKVIMAMKEIRLELDETKFRQ
ILMELEILHKCDSPYIVDFYGAFFVEGAVYMCMEYMDGGSLNNIYQGGVEDRYLAIIT
DSVVRGLKKLKDEHNIIHRDVKPTNILVSTSGKVKLCDFGVSGNLVASIARTNVGCQS
YMAPERIRSKNPNEAITYTVESDIWSLGLSLIEIARGEYPYPADTHNSIYSQLMAILD
GEPPCLPDDKYSPEARAFVAQCLNKTARLRPSYAELLKHPWLVNIEQELAHSPTLEVE
FGAFVKRSVERNNHREN"
gene <200119..>201759
/gene="ANR2"
/locus_tag="AWJ20_2036"
/db_xref="GeneID:30033903"
mRNA <200119..>201759
/gene="ANR2"
/locus_tag="AWJ20_2036"
/product="Anr2p"
/transcript_id="XM_018878962.1"
/db_xref="GeneID:30033903"
CDS 200119..201759
/gene="ANR2"
/locus_tag="AWJ20_2036"
/note="hypothetical protein; may have a role in lipid
metabolism, based on localization to lipid droplets;
predicted to be palmitoylated; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0005811 - lipid particle [Evidence
IDA] [PMID 24868093]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008150 -
biological_process [Evidence ND]"
/codon_start=1
/product="Anr2p"
/protein_id="XP_018736923.1"
/db_xref="GeneID:30033903"
/translation="MSGHPRKKLAIGAFLIKFDMRTGNSLIWSKSQPGIEIDWEGVEF
MAMPSGLHSRQSDVIRFVLSNPLERVTRTGSTRDRLLGDEAFGYLKNNIEGIAVFNQN
FHRGDATTTRINVRSTIQMYSLGVLCTKSDSEISSKCWGFVDILTELLTTFMNLNQDN
HQVHETLDFRPFESLLQYDDNGELQTLSLSPPRLSAKPLNLAPHHPILSTTELLVTYG
PLIFEIWKAALLRDKILLMDKSNSISVDDMCKFVYIISVFGTVPNDVFDLIPRNKQHS
LSTATNPIYNVNVSDITSLSHLKHFIATTTDEILLEKEYLYNMTVVKTGDRAIAATTS
TTDFYGNLNTMPISAGFHDKRRFQILISGLGIETPHHTSGQSILAHVCSNISSLFSLT
SLYGLLWWATAGEQANSDTDRYLEGLPLLTQTQVESSSHVAGQRASSYATESPYFNAI
SELSPPQPSATMEPWQISVIGYFQAKTRRIFETVASIVESQDQDQSETSLQTVISIEP
PDLMDMGLDPISLRDHEFVVKFISLWWGRPARVNHVCC"
gene complement(<201957..>204599)
/gene="LHS1"
/locus_tag="AWJ20_2037"
/db_xref="GeneID:30033904"
mRNA complement(<201957..>204599)
/gene="LHS1"
/locus_tag="AWJ20_2037"
/product="Hsp70 family chaperone LHS1"
/transcript_id="XM_018878963.1"
/db_xref="GeneID:30033904"
CDS complement(201957..204599)
/gene="LHS1"
/locus_tag="AWJ20_2037"
/inference="similar to AA sequence:KEGG_Orthology:K09486"
/note="Molecular chaperone of the endoplasmic reticulum
lumen; involved in polypeptide translocation and folding;
nucleotide exchange factor for the ER lumenal Hsp70
chaperone Kar2p; regulated by the unfolded protein
response pathway; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005788 -
endoplasmic reticulum lumen [Evidence IEA]; GO_component:
GO:0005788 - endoplasmic reticulum lumen [Evidence IDA]
[PMID 8654361]; GO_component: GO:0005788 - endoplasmic
reticulum lumen [Evidence IDA] [PMID 8887673];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0005524 - ATP binding [Evidence IDA,IMP]
[PMID 19759005]; GO_function: GO:0016887 - ATPase activity
[Evidence IEA]; GO_function: GO:0000774 -
adenyl-nucleotide exchange factor activity [Evidence IDA]
[PMID 14704430]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0051082
- unfolded protein binding [Evidence IMP,IPI] [PMID
10409721]; GO_function: GO:0051082 - unfolded protein
binding [Evidence IGI,IMP] [PMID 8654361]; GO_function:
GO:0051082 - unfolded protein binding [Evidence IGI,IMP]
[PMID 8887673]; GO_process: GO:0006200 - ATP catabolic
process [Evidence IEA]; GO_process: GO:0008152 - metabolic
process [Evidence IEA]; GO_process: GO:0031204 -
posttranslational protein targeting to membrane,
translocation [Evidence IMP] [PMID 8654361]; GO_process:
GO:0031204 - posttranslational protein targeting to
membrane, translocation [Evidence IMP] [PMID 8887673];
GO_process: GO:0006986 - response to unfolded protein
[Evidence IGI,IMP] [PMID 8654361]; GO_process: GO:0006986
- response to unfolded protein [Evidence IGI,IMP] [PMID
8887673]"
/codon_start=1
/product="Hsp70 family chaperone LHS1"
/protein_id="XP_018736924.1"
/db_xref="GeneID:30033904"
/translation="MHIKLFKSTFLGFLFILFTSLQLIQCAVLGIDFGQEYTKGSLVA
PGAPFEVIMTSDSKRKDLSGITFKKAPGSGSGTFERIYGNSAAAVIQRFPATSPFYLK
PLLGQSLDSPAAKWYSEQFPAVKLVPSNNNRSTIAIQIFDDIYSVEEIVAMSFSEIKG
RASKVLTQALSGSSGYLKDTALSVPPYYSIQQRKALQDAVEIADLRLISLVNDGAAVA
ANFAASRQFENDKKEHHIIYDIGAGSTTATLVEIYQADLVPEGSPFPRSHTVIDVIGV
GYDTELGGHVLTQSVRNILIDKFLEANPKVKRNDLLNDHRALARFWREAERVKFVLSA
NSETQSSIESAYNDISLKTHVSRVEFEDANRHLFDKVPAPILDALNNPLNNSTEAFDF
SKVSSIILTGGSTRIPIVQQELLSLINARDPTEQVTLAKNVNADEAAVLGTALRGVGI
SKIFRSKDMAVIDRAIWDYSAEIDGEVATIFPRGTPLDSKFVLPFDSSKDDFKLVLYE
NGKEFVRHNINGVQSAIKRLLNDPHRECISNVSVEAEFHLSNSRIVELLGTYAKCESI
EKVVSTESSQQSSTSDSADVESSSETDNASSGTTSSATTASVSPTAKPPKVVKKSVSM
TNSFDGPRPMGSASKQSALSRLRALDRLDADRLSREEARNTFESRLYRVRELLESEES
AQQKLAEFGEWFDYDSEKASIKVLQEKLAELNALADSYNKETTVQQIELPKETGSVVE
PTMAGPEIILEPPVVQEQQPFENEKLADVYQGIKEVAEMLDQLGIDIPNREAVAQILG
GSTVPDSTSNLKGIAELLRSKLQAANLDGSEPADSGDVESRLNDLRHMIKKAADIIKT
ESSATPSVSISTSPSPKNPQHDDL"
gene <206591..>213319
/gene="URA2"
/locus_tag="AWJ20_2038"
/db_xref="GeneID:30033905"
mRNA <206591..>213319
/gene="URA2"
/locus_tag="AWJ20_2038"
/product="bifunctional carbamoylphosphate
synthetase/aspartate transcarbamylase"
/transcript_id="XM_018878964.1"
/db_xref="GeneID:30033905"
CDS 206591..213319
/gene="URA2"
/locus_tag="AWJ20_2038"
/inference="similar to AA sequence:KEGG_Orthology:K11541"
/note="Bifunctional carbamoylphosphate
synthetase/aspartate transcarbamylase; catalyzes the first
two enzymatic steps in the de novo biosynthesis of
pyrimidines; both activities are subject to feedback
inhibition by UTP; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11015727]; GO_component: GO:0016021 -
integral component of membrane [Evidence IDA] [PMID
11921093]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0016597 - amino acid binding [Evidence IEA];
GO_function: GO:0004070 - aspartate carbamoyltransferase
activity [Evidence IEA,IEA]; GO_function: GO:0004070 -
aspartate carbamoyltransferase activity [Evidence IDA]
[PMID 10446140]; GO_function: GO:0004070 - aspartate
carbamoyltransferase activity [Evidence IMP] [PMID
4550660]; GO_function: GO:0004088 - carbamoyl-phosphate
synthase (glutamine-hydrolyzing) activity [Evidence IEA];
GO_function: GO:0004088 - carbamoyl-phosphate synthase
(glutamine-hydrolyzing) activity [Evidence IDA] [PMID
5776390]; GO_function: GO:0016743 - carboxyl- or
carbamoyltransferase activity [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA,IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0044205 - 'de
novo' UMP biosynthetic process [Evidence IEA]; GO_process:
GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic
process [Evidence IEA]; GO_process: GO:0006207 - 'de novo'
pyrimidine nucleobase biosynthetic process [Evidence IDA]
[PMID 181668]; GO_process: GO:0006207 - 'de novo'
pyrimidine nucleobase biosynthetic process [Evidence IMP]
[PMID 4550660]; GO_process: GO:0070409 - carbamoyl
phosphate biosynthetic process [Evidence IEA]; GO_process:
GO:0006520 - cellular amino acid metabolic process
[Evidence IEA]; GO_process: GO:0006543 - glutamine
catabolic process [Evidence IEA]; GO_process: GO:0006541 -
glutamine metabolic process [Evidence IDA] [PMID 5651325];
GO_process: GO:0008152 - metabolic process [Evidence
IEA,IEA]; GO_process: GO:0045984 - negative regulation of
pyrimidine nucleobase metabolic process [Evidence IDA]
[PMID 5651325]; GO_process: GO:0006807 - nitrogen compound
metabolic process [Evidence IEA]; GO_process: GO:0006221 -
pyrimidine nucleotide biosynthetic process [Evidence IEA]"
/codon_start=1
/product="bifunctional carbamoylphosphate
synthetase/aspartate transcarbamylase"
/protein_id="XP_018736925.1"
/db_xref="GeneID:30033905"
/translation="MSSDKIEELPVVSATPSLPIFPPLHNTDKLVTLELKNGISVQGY
SFGADKSVAGELVFQTGMVGYPESITDPSYEGQILVITFPLVGNYGVPSRDEMDELVP
QLPRYFESNRIHVAGLVVASYTEEYSHFLAKSSLGTWLKEEGIPAIYGVDTRGLTKLI
RTQGSTLSRIRLQKEKVNPALSWDENFEEVEYDDPNVKNLVARVSIKEPVLYKPASSV
TPVVDSTGKTLRILAIDVGMKYNQVRCFVNRGVELKVVPWDYDFVGAGEQYDGLFVSN
GPGDPATLTSVVKRLETVVEQAKTPIFGICLGHQLLASASGASTLKLKFGNRGHNIPA
TNLISGRCYITSQNHGYAVDVNTLKEGWKELFVNANDGSNEGIYHTSKPIFSVQFHPE
STPGPRDTEFLFDVFINTVLKHKNAGGKPIGPVEFPGGTIEENRAKHPRVHAKKVLVL
GSGGLSIGQAGEFDYSGSQAIKALKQEGIYTILINPNIATIQTSKGLADKVYFLPVNA
DFVRKVIKYENPDAIYVTFGGQTALNVGIQLKDEFESLGVKVLGTPIDTIITTEDREL
FARSMEEIGEKCAKSATASTTAEALEVVKEIKFPVIVRAAYALGGLGSGFAENEEQLV
ALCQKAFAASPQILIERSMKGWKEIEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIV
VAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSS
ALASKATGYPLAFIAAKLGLNIPLNEIKNSVTKVTCACFEPSLDYVVVKIPRWDLKKF
TRVSKELSSSMKSVGEVMSIGRTFEEAIQKAIRSTDYQNLGFNATEALMSIDIDDELQ
TPSDQRLFAIANAFDSGYTVDKVWSLTKIDKWFLHKLENLIKFAKMMEQFQSKTIPST
TMRQAKQLGFEDRQIAKFVSSNELSIRRLRVEAGITPFVKQIDTVAAEFPAFTNYLYL
TYNADSHDIDFNDNGVMVLGSGVYRIGSSVEFDWCAVRAVRTLREQGTKTIMVNYNPE
TVSTDYDEADRLYFEVINLERVLDIYQLEQSAGVVISMGGQTSNNIALTLHRENVKIL
GTSPDMIDSAENRYKFSRMLDKIGVDQPAWKELTSIEEAEAFADKVSYPVLVRPSYVL
SGAAMNTVYSRDDLESYLKQAVEVSRDYPVVITKYIENAKEIEMDAVAKDGKLVMHVV
SEHVENAGVHSGDATLIVPPQDLSAETVKRIVEATAKIGQALNVSGPYNIQFIAKDNE
IKVIECNVRASRSFPFVSKVLGVDLIEMATKVIAGVPIQPYPLQQLPEDYVAIKVPQF
SFSRLSGADPVLGVEMASTGEVACFGRNKYEAYLKSLIATGIKPPKKNILLSIGSFKE
KTELLPSVKKLHELGYKLFATAGTADFIQEHGIPVQYLEVLNEGEDEKSEFSLTQHLA
NNLIDMYINLPSSNRFRRPASYMSKGYRTRRMAVDYAIPLVTNVKCAKLLVEALALNI
PLEVSSIDSQSSHRLLSLPGLINIATFVPSLLSAGGSPDFEKVSEASLKAGFTITRIL
PTGVDGSITDALSLLTAQENTRGKSAVDFNLSIAATGDNASQLVKASPNVGSLVIPFN
YFNNNLDKVATVSEHFNVWPTSKPIVTDASSTDLASILLLASLHNRPVHVTNVLKKAD
LALISMAKEKGLKVTCDVSIHALFLSQVEFPGCSHLPTKEDQEGLWEGLESIDIFAVG
VLPSQLAKSLGLTVEAGVGIADALPLLLTAVRENKLTVEDIVLRLSTNPKKIFDLHDQ
SGTTVEVDLDRQTTVSSFYSKPLYGTVERVTYNDKTVVLEGKIVVSDFHLGKDVSGHS
NAAQVVPASPLVKGGAVFASPRPASPTGVSGFNAQARKMSFNAVSVVAPGGRADLDSA
LVLEDDDKSKGPTLKLVTEPQLPSALRNVIRKNNPFLRRHVTSVLDFSRSDLHSLFSL
AQELRLAVERVGAVDLLRGRVLATLFYEPSTRTSTSFDAAMQRLGGKVISITTSTSSV
QKGETLQDTVRTLASYTDAIVLRHPSEESVAIASKYSPVPIINGGNGSKEHPTQAFLD
LFTIREELGTVNGLTVTFMGDLKYGRTVHSLCSLLKHYQVRVQIVCPKDLALPAHVYD
ELAKAGKITVVSENLTDEILENTDVLYCTRVQKERFPSLDEYEKFKNLYVVDNKVLRK
AKKHMCVMHPLPRVNEISEEVDFDHRAAYFRQMRHGLFVRMALLAMVIGVDF"
gene <214221..>215399
/locus_tag="AWJ20_2039"
/db_xref="GeneID:30033906"
mRNA <214221..>215399
/locus_tag="AWJ20_2039"
/product="hypothetical protein"
/transcript_id="XM_018878965.1"
/db_xref="GeneID:30033906"
CDS 214221..215399
/locus_tag="AWJ20_2039"
/inference="similar to AA sequence:KEGG_Orthology:K12837"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736926.1"
/db_xref="GeneID:30033906"
/translation="MAKASGLFPTSSDIRKLNERRDGGDNDKGSMAEPASLMLPPPPP
TILTAGSNEYNVSPQGSRLAKLVILSGYDAEAQSIEMLTKLFITLLLSITDSGATADS
VKDCFYANGSDKTKLVIELAKPELATIAVAFDGVALDESEPDTTKFSITRPEGYIIPL
SSDNIEGAKDDKKTKFDVEGPFKGSMSNIPTFIGEEQILELLKAFGQVRFCKILTDVA
GNSKGVAFFEYEDTSILDIACQGLNNMALGDNQLIVRKACVGLEQSQPVMSLDTLLKL
CQDNDRKNEHPPTSVMQLWNAISPEELADDEAREEISAQIEQECSKFGPVESVNIPKE
NSASSTMLSSSIGGRIYVKFSSSSACKEAVKHLGGRKFTDRTIISGFYHEDAYKLGIL
"
gene <218687..>221182
/gene="SEC27"
/locus_tag="AWJ20_2040"
/db_xref="GeneID:30033908"
mRNA <218687..>221182
/gene="SEC27"
/locus_tag="AWJ20_2040"
/product="Sec27p"
/transcript_id="XM_018878967.1"
/db_xref="GeneID:30033908"
CDS 218687..221182
/gene="SEC27"
/locus_tag="AWJ20_2040"
/inference="similar to AA sequence:KEGG_Orthology:K17302"
/note="Essential beta'-coat protein of the COPI coatomer;
involved in ER-to-Golgi and Golgi-to-ER transport;
contains WD40 domains that mediate cargo selective
interactions; 45% sequence identity to mammalian
beta'-COP; GO_component: GO:0030126 - COPI vesicle coat
[Evidence IGI,ISS] [PMID 7929113]; GO_component:
GO:0030663 - COPI-coated vesicle membrane [Evidence IEA];
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0031410 - cytoplasmic vesicle [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0030117 - membrane coat [Evidence IEA];
GO_function: GO:0005198 - structural molecule activity
[Evidence IEA]; GO_function: GO:0043130 - ubiquitin
binding [Evidence IDA] [PMID 21070969]; GO_process:
GO:0006888 - ER to Golgi vesicle-mediated transport
[Evidence IMP] [PMID 7929113]; GO_process: GO:0006886 -
intracellular protein transport [Evidence IEA];
GO_process: GO:0032511 - late endosome to vacuole
transport via multivesicular body sorting pathway
[Evidence IMP,IPI] [PMID 17101773]; GO_process: GO:0015031
- protein transport [Evidence IEA]; GO_process: GO:0006890
- retrograde vesicle-mediated transport, Golgi to ER
[Evidence IMP] [PMID 8001155]; GO_process: GO:0006810 -
transport [Evidence IEA]; GO_process: GO:0016192 -
vesicle-mediated transport [Evidence IEA,IEA]"
/codon_start=1
/product="Sec27p"
/protein_id="XP_018736927.1"
/db_xref="GeneID:30033908"
/translation="MNYETQVVVKTIDVSHLPVRAGRFVARKNWIVLGSDDYMVRVFN
YNTGEKVTQFEAHPDYIRVLVVHPTQPFVLTAGDDMTIKLWNWDQGWRNTQTFEGHSH
YVMYLAFNPKDPNTFASACLDKTVKIWSLGSSTPNFTLEAHETKGANFVEYYPQSDKP
YLITTSDDRTIRVWDYQTKSCVATMADHMNNVSFAVFHPELPVIISGSEDSTIKIWNA
NTYKLEQTLNYGLERAWCAACKKGSNLMALGFDAGSVVLQLGKEEPAISMDPNGRLIW
SKHSEVFSSVVKGGDDVNDGDFLPIAQKDLGNVEVYPTQLNHSPNGRFVAVTGDGEYI
IYTALAWRNKSFGSGLDFAWAQDSNEYAVRESTSSIRLFKNFKERPIGHLNIGYSATQ
IYGGTLLGINGGEFVAFYDWESGLLVRRIDVEASQVVWSDSGELVAIITDDSFYILRF
SRDAFQLAVQNNSVSEDDGVEESFEVAYDISDSVRTGKWVGDCFIYTTFSNRLNYLVG
GEVYTISHFDKQMYLLGYIPRDNAIYLADKDVNVTAYKLSLAVVEYQTVVLRGDMDYA
AEILEKVPDNEKTKVARFLEAQGYPELALEVSRDSDHRFDLAVSLDKLDIAEEIASSV
DDEHKWKSLGDKSLLSWNVILAEKSFKAAHDFESLLLIYTSTGNKPGAAELAEEAVKA
GKYNLAFNAYWYVGDTDACIKLLNDTGRSSEAALFALTYGGDVDGSVKKWKDGLVKSG
RSKIAQSISEPSQDKELFPANVGQKVTTLPSIGGDLIDVDDIATPEPEPQSAEEAELI
EVEAEAEPESDPTAEDKAEETEDADAEVEKDSL"
gene <221497..>222978
/locus_tag="AWJ20_2041"
/db_xref="GeneID:30033909"
mRNA <221497..>222978
/locus_tag="AWJ20_2041"
/product="hypothetical protein"
/transcript_id="XM_018878968.1"
/db_xref="GeneID:30033909"
CDS 221497..222978
/locus_tag="AWJ20_2041"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736928.1"
/db_xref="GeneID:30033909"
/translation="MSIVKIQNGLFRKLGEGSKRTLIFKSPLSLDIKSTDRWAITGPR
KTELLEILASKHIPDPPLSRTYPFLANQNAWPSQVTQLIEFSNADVKAPHISARYESL
REELDETLNESLLAATHSKDQAKVDHVLEKFSLKGIENRWIVGLSNGQSRRARLAKAL
LRDPKFLLVDEPFVGLDPFSRKQASDILSKLSPEPHVVVGLRYQDEFPSWITHVAITD
RTGNGISHQGPIKDLAPYLDEMRRDHIVQVVKAKDLAVKAAEKSKLAASNNPEVIKLD
SVSVSYKGEPIFSNLTWKVKKGEKWHLRGDNGTGKSTLLSLLTGDHPQSWNPKISLFG
EPRGTGKQSYFSINEKFGHVSPEIHALFPMNLTVKEAISTGFVVGYYIPLKKLSQEQE
DRIDELLSLFEHISKDEVLRDLSVSNQKIVMFLRGLAKRPDILILDEAFSAMAEESID
KCKQYIDLEYDGTLIVVGHIEDEVPLCHKYIRLTAAEPEFGEL"
gene complement(<223075..>223743)
/locus_tag="AWJ20_2042"
/db_xref="GeneID:30033910"
mRNA complement(<223075..>223743)
/locus_tag="AWJ20_2042"
/product="hypothetical protein"
/transcript_id="XM_018878969.1"
/db_xref="GeneID:30033910"
CDS complement(223075..223743)
/locus_tag="AWJ20_2042"
/inference="similar to AA sequence:KEGG_Orthology:K07734"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736929.1"
/db_xref="GeneID:30033910"
/translation="MYIPKYNVEPSEELQIQLIREFPLGTIITSSADGIIANHLPFLV
DKTSDGLVLRAHFARVNHQRDDFRSGKELLVVFKGLDGYETPNWYVDTKPTTGKVVPT
WDFSAVHVYGVPTVIEDPAWIYKQFTDLSNMNEREQSEPWTVDQAPESYVNLLQKAIW
GLEIKVTRVESKWKVGQKLSDKDNRGVIEGFKTQGEKGAALSEVVEKAKIRHDERRKN
GLQT"
gene complement(<225405..>226148)
/locus_tag="AWJ20_2043"
/db_xref="GeneID:30033911"
mRNA complement(<225405..>226148)
/locus_tag="AWJ20_2043"
/product="hypothetical protein"
/transcript_id="XM_018878970.1"
/db_xref="GeneID:30033911"
CDS complement(225405..226148)
/locus_tag="AWJ20_2043"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736930.1"
/db_xref="GeneID:30033911"
/translation="MRFSYATSALLAVAATSVNAALVTQYVQVTVDVYEQAHVFVNGN
GDPVSTSYELVSSEESAPTQVPSSHQHSDAQPVQIQLTTSTAAASSSPAPPPPAPTTS
SSTTTPPPPPPPTTTSSPPPPPPAPSSSSASSDSSDSSSSSGSGEFSGQGTFYSPGMG
SCGIESSDSDFIAALNAPQMGSPANPNANPNCGKKAIVYSGGKSVTVTIVDKCPSCAY
GSLDLSPAAFNELADPSAGRIDISWSWAS"
gene complement(<227499..>229136)
/gene="HNM1"
/locus_tag="AWJ20_2044"
/db_xref="GeneID:30033912"
mRNA complement(<227499..>229136)
/gene="HNM1"
/locus_tag="AWJ20_2044"
/product="Hnm1p"
/transcript_id="XM_018878971.1"
/db_xref="GeneID:30033912"
CDS complement(227499..229136)
/gene="HNM1"
/locus_tag="AWJ20_2044"
/note="Plasma membrane transporter for choline,
ethanolamine, and carnitine; involved in the uptake of
nitrogen mustard and the uptake of glycine betaine during
hypersaline stress; co-regulated with phospholipid
biosynthetic genes and negatively regulated by choline and
myo-inositol; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA,IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA,IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence ISS] [PMID 2203793]; GO_function:
GO:1901235 - (R)-carnitine transmembrane transporter
activity [Evidence IMP] [PMID 23755272]; GO_function:
GO:0015171 - amino acid transmembrane transporter activity
[Evidence IEA]; GO_function: GO:0015220 - choline
transmembrane transporter activity [Evidence IMP] [PMID
2203793]; GO_function: GO:0015220 - choline transmembrane
transporter activity [Evidence IGI] [PMID 3514579];
GO_function: GO:0034228 - ethanolamine transmembrane
transporter activity [Evidence IGI] [PMID 3514579];
GO_process: GO:1900749 - (R)-carnitine transport [Evidence
IMP] [PMID 23755272]; GO_process: GO:0003333 - amino acid
transmembrane transport [Evidence IEA]; GO_process:
GO:0006865 - amino acid transport [Evidence IEA,IEA];
GO_process: GO:0015871 - choline transport [Evidence IMP]
[PMID 2203793]; GO_process: GO:0015871 - choline transport
[Evidence IGI] [PMID 3514579]; GO_process: GO:0034229 -
ethanolamine transport [Evidence IGI] [PMID 3514579];
GO_process: GO:0031460 - glycine betaine transport
[Evidence IMP] [PMID 16487344]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Hnm1p"
/protein_id="XP_018736931.1"
/db_xref="GeneID:30033912"
/translation="MVEEEKKRLSVESLRSGQQQKLDAFDLADMGHAQELPRQFSMWS
MLALAFSVLGTWATFAQDLMSGLQNGGSIAILWGLALVTICNICVAVSLGELCSSMPT
ALGQAYWIHRLWNTPAGRFSSYICAWINTFGWWTLTASQIAFMTNFILGIKVMFDNDW
AGASEGWVQFLVYVGITVVFTLTNVLACRKDNFLAWFNNVVGIQFTMLFVIFSLAMLI
AVGVHHDKHFQTAGFVFGKWLNETGWPDGVVWFTGLLQAAYGLTAFDSVIHMVEEIPS
PRVNAPRVIYLAVIMGAITGGIFMVVCLFCIQDLDSILNSPTGLPFMQLLTDTVGVQG
GAALLSIFVFNGLGQGVSVATTASRLTWGFARDGGLPFSQYFAHVDTYWHVPARSLWA
QGVIIAVVGVLYLFASTVLEAILSVSTIALTISYGMPIAALVLVGRDKLTPGPFHLGR
YGMFCNIVSIIYCCVTTVFFFFPGGPDPAPADMNYAIAVFGVMMVIALAFWFIKGRRT
YLLTDEALIEMLRARELENSANDATVVVVDGQAPEKN"
gene <230964..>231419
/locus_tag="AWJ20_2045"
/db_xref="GeneID:30033913"
mRNA <230964..>231419
/locus_tag="AWJ20_2045"
/product="hypothetical protein"
/transcript_id="XM_018878972.1"
/db_xref="GeneID:30033913"
CDS 230964..231419
/locus_tag="AWJ20_2045"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736932.1"
/db_xref="GeneID:30033913"
/translation="MPPKTSAAELTIRLKHKKQTVLLILAGGVTVNGLKKTLAKALNE
TRGSDQGVDEVDDAPIPKPSFETASDVEEDTDKQPGAEEITAKIIDANDLRVGIPVDK
SDFSKGFTDITDAKGNIEANGIVEGSILAFCTKDGDFEIEVPYDDNLDE"
gene <234659..>235537
/gene="RTR1"
/locus_tag="AWJ20_2046"
/db_xref="GeneID:30033914"
mRNA <234659..>235537
/gene="RTR1"
/locus_tag="AWJ20_2046"
/product="Rtr1p"
/transcript_id="XM_018878973.1"
/db_xref="GeneID:30033914"
CDS 234659..235537
/gene="RTR1"
/locus_tag="AWJ20_2046"
/note="CTD phosphatase; dephosphorylates S5-P in the
C-terminal domain of Rpo21p; has a cysteine-rich motif
required for function and conserved in eukaryotes;
shuttles between the nucleus and cytoplasm; RTR1 has a
paralog, RTR2, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 18408053]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 18408053];
GO_function: GO:0008420 - CTD phosphatase activity
[Evidence IDA] [PMID 19394294]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0046872
- metal ion binding [Evidence IEA]; GO_function:
GO:0004721 - phosphoprotein phosphatase activity [Evidence
IEA,IEA]; GO_process: GO:0070940 - dephosphorylation of
RNA polymerase II C-terminal domain [Evidence IDA,IMP]
[PMID 19394294]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IGI,IMP,IPI] [PMID 18408053]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Rtr1p"
/protein_id="XP_018736933.1"
/db_xref="GeneID:30033914"
/translation="MSDTIFSMDTGDSTAHPKFATQQDAVINGVTHNVSANNSPSQSL
IEMCRIIAKYSKPGFLSAGEASQILLSIVELMSDGVESDALKFSSRFLTGSDYEDIVV
ERNILHRCGYPICDKDPKGIHKAHQINYRTPKMILPSTYLSKYCTKEHYQASTFFASQ
LSDASLFSRKDVTLLPYGASTEYELQIALMEEVQVLSQSQNRSMQDIIQELKSLSLSK
QQSQIDTLPSTQPRRYSSANPADPHLVDLSDQIKAFKIVERDPSIPTLDAGDEPIEEA
IASGVEGYNSRFGRSV"
gene <236924..>238288
/gene="HXT13"
/locus_tag="AWJ20_2047"
/db_xref="GeneID:30033915"
mRNA <236924..>238288
/gene="HXT13"
/locus_tag="AWJ20_2047"
/product="hexose transporter HXT13"
/transcript_id="XM_018878974.1"
/db_xref="GeneID:30033915"
CDS 236924..238288
/gene="HXT13"
/locus_tag="AWJ20_2047"
/inference="similar to AA sequence:KEGG_Orthology:K08139"
/codon_start=1
/product="hexose transporter HXT13"
/protein_id="XP_018736934.1"
/db_xref="GeneID:30033915"
/translation="MVDEKIQVENVEHVVYDSPEKEIVEFRGSDALLEAAQKDPPKIF
SKRMLQLYIICLIPFLCSTMNGYDGSLLSSLLLLKPFNDTFGATTVGIKAGLISAMYQ
IGGVATIPFLGPVNDTFGRRFGMFFGCLIVVIGTVVQATSKNINQLMGGRFVLGFGVS
FATAAAPIYAIEISHPSYRAAITGSYNTLYCWGSILATVVLFCTQNIPNNRAWIIPTY
VQMVCPGLVVIFSMFLPESPRWLYCNGKQEKAKEILTYYHGEGNPENAFITLQIREFE
EQLELDGTDKRWWDYRALFRDRASIYRLCCNLVYSCFGQLSNGGISYFVGAFYHSAGI
TSNRTIFSYNLGTGFLSFILALIGAQVIQQIGRRKVILGSLVGMTLFWALVTICVARF
DASGEKTTAWAQAGIAFYILFGVVYGKLRCCLFRQVYILTKTQPSRLLLCKHFILMKS
FHTK"
gene <242372..>244669
/gene="SIP5"
/locus_tag="AWJ20_2048"
/db_xref="GeneID:30033916"
mRNA join(<242372..242725,242918..>244669)
/gene="SIP5"
/locus_tag="AWJ20_2048"
/product="Sip5p"
/transcript_id="XM_018878975.1"
/db_xref="GeneID:30033916"
CDS join(242372..242725,242918..244669)
/gene="SIP5"
/locus_tag="AWJ20_2048"
/note="hypothetical protein; interacts with both the
Reg1p/Glc7p phosphatase and the Snf1p kinase; forms
cytoplasmic foci upon DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IPI] [PMID
10628972]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 22842922]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0042149 -
cellular response to glucose starvation [Evidence IMP,IPI]
[PMID 10628972]"
/codon_start=1
/product="Sip5p"
/protein_id="XP_018736935.1"
/db_xref="GeneID:30033916"
/translation="MGNVPTKDVSATGSGSNSRSRSASTATAESSTTTASSSRRTRLS
STSSRSTVRDETSGFGTSVTGLSRKSGKKKEKSSASDAPLVSDVSEMVDGGYLVPQGV
YSGPQDFKAKVVRNLIIERRLAPFYKGIEDYDDTWTDRQLLAAVRNLPIPPATDPVVS
PTTSSDQAEPVEEPDRKKKEKEADTPVDPATETDESAEQGYESENALKWVSSSLPNAV
GGSFLQARNSDDGGSSGRLAGFESNPSIRSSSSRSSDPDDGILSPPSNGKQKQPMTPP
LAASAEEDETIPFPSSPASSSSGAMRIIEFKTETMHIEDTATGTNSISTNASARITSG
GGISIETRPREGSAKLGPPILLPRNRANTTSRYGPGPTSSSMKPAPPPMEVILYRGAI
ECPICFLNYPKYLNTTRCCTQPICSECFVQIKRPDPHPPYHENNGSGPSTAPATDNPE
EALVSEPACCPFCQMPEFGVVYIPSPHRSGIPASSLTSSLRAHLSSSLSGVFSSHHHT
PVSSSAASIVSMSTSNSATGTPRRRGSLPPNAPEVVTTDHVRPDWAMKLSRARHQLAR
KSAAANALHASAFLFEGNEGAASSSSSHSRRHDKKKKSSSRLRGLSSPSSPASILGSS
SSGSGSSRRHTTSSPSSGTSFPVPSRTRIRDLEDMMIVEAIRRSLTDERSGSPSGESP
KAPASQSSQEVKKDEITTQ"
gene <250855..>252030
/gene="HAC1"
/locus_tag="AWJ20_2049"
/db_xref="GeneID:30033917"
mRNA <250855..>252030
/gene="HAC1"
/locus_tag="AWJ20_2049"
/product="transcription factor HAC1"
/transcript_id="XM_018878976.1"
/db_xref="GeneID:30033917"
CDS 250855..252030
/gene="HAC1"
/locus_tag="AWJ20_2049"
/codon_start=1
/product="transcription factor HAC1"
/protein_id="XP_018736936.1"
/db_xref="GeneID:30033917"
/translation="MVVVKSEPKDLYLFSDESLQYGDDTLIDDKDALNDDEDNMNETD
ELQDQDCSLSDRQMSEEHDLDDSDMVTTESIDAATGLTITTTVGSGANKRKEVMTLPL
PPGSLPPRKRAKTKDEKEQRRIERIMRNRQAAHASREKKRKHVEELEKKCVVLTSENE
QLQQNMRQMKESQVQMLEQHYLLMAKVQQLQNVLKTAKTTGDLSVLDSADFTVSLPSM
PTATLLSQGSSDMVSPVSTSTTTTGTSTPGTSVDDSSERDKLHTKASRSSSPLSSSSS
SSTMLLSSTVKEEECTSPEALLLSNVITTAPSLSNSPLQSDNLDFDDSSNESTISTPP
SHNTYIKQELFEDDKDSYNFENSNQAHHPAAVMCDQQRLRLTKFSPLSESLPQSITV"
gene <254820..>256241
/locus_tag="AWJ20_2050"
/db_xref="GeneID:30033919"
mRNA <254820..>256241
/locus_tag="AWJ20_2050"
/product="hypothetical protein"
/transcript_id="XM_018878978.1"
/db_xref="GeneID:30033919"
CDS 254820..256241
/locus_tag="AWJ20_2050"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736937.1"
/db_xref="GeneID:30033919"
/translation="MRAAVNLHINITDDMRGGVGGGGGGMPGYAMTRTPSNSSVNSTS
SNPPPLTPINQTIGTPTINRQFMFHRLNSVSSIATSEDDSDVNARLDNPLDSFLDWPS
QDDDLLMNIYQAYIDNPTTAPFAGKIPPSGVVHQVAKTTLHEAKKKSRPFLHSMLAVR
KRLLLLCERNSAPTQIYTRASSTSSLSSNSSFNFSNAPAITPGTIPPNRFFRQLSFGS
NGSPESPVMTPLANRIKRPCTGFANGTSPGEAHAITTNDGVSLSAFMLGHPDDYDEEM
AFADESPLGTSLLPPPPTFRIPEYENSNNNNSNNNAGPQSPIDIQPSPFDRKTRQGST
SSRGSPKLGSQSQNVPPQQPNAYSSQTQQNIANWLLNTPNHHGQATTSGLGMPLTPAP
SPLNIKNAHRASVGTSAQANPDDFDFGLDSPFKEMTVTPTRPHQQSPGSQPSTPVEDP
YPNYTAQRKRESLRMKRGMKAPI"
gene <256745..>257827
/gene="GLE2"
/locus_tag="AWJ20_2051"
/db_xref="GeneID:30033920"
mRNA <256745..>257827
/gene="GLE2"
/locus_tag="AWJ20_2051"
/product="RNA export factor GLE2"
/transcript_id="XM_018878979.1"
/db_xref="GeneID:30033920"
CDS 256745..257827
/gene="GLE2"
/locus_tag="AWJ20_2051"
/inference="similar to AA sequence:KEGG_Orthology:K14298"
/note="RNA export factor associated with the nuclear pore
complex (NPC); associates with NUP116p; required for
polyadenylated RNA export but not for protein import;
homologous to S. pombe Rae1p and human RAE1; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 15210706];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0031965 -
nuclear membrane [Evidence IEA]; GO_component: GO:0005643
- nuclear pore [Evidence IEA,IEA]; GO_component:
GO:0005643 - nuclear pore [Evidence IDA] [PMID 10684247];
GO_component: GO:0005643 - nuclear pore [Evidence IDA]
[PMID 8970155]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0051028 -
mRNA transport [Evidence IEA]; GO_process: GO:0031081 -
nuclear pore distribution [Evidence IMP] [PMID 9725905];
GO_process: GO:0016973 - poly(A)+ mRNA export from nucleus
[Evidence IMP] [PMID 8970155]; GO_process: GO:0000973 -
posttranscriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [Evidence IMP] [PMID 20932479];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:2000728 - regulation of mRNA export from
nucleus in response to heat stress [Evidence IMP] [PMID
15210706]; GO_process: GO:0000972 -
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery [Evidence IMP] [PMID
20098417]; GO_process: GO:0000972 -
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery [Evidence IMP] [PMID
20932479]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="RNA export factor GLE2"
/protein_id="XP_018736938.1"
/db_xref="GeneID:30033920"
/translation="MSGLFASRSVGTTSNGPAPSSELMLQNDRTLANPPEDSISDMSF
SSQADYLAVASWDKKVRIYEVSPQGDSQGRAMYEHQAPVLTAQWFQDGSKVVSGGCDN
AVRAHDLQSSQTIQIGQHDAPVSSVRAVDVGSGSPVVASASWDKTLRYWDLRQQQAIS
TIQLPERAYSMDSQGKLLVVGTAERHICVIDLSNPGQIFKNTQSPLKWQTRVVACYPS
GNGYAVTSIEGRCAIQYVDDAEQAKLGFSFKCHRDQATPPRNDNLIYSVNGVSFHPVY
GTFCTAGSDGTVHFWDKDSRSRLKFSRPVGGTISSTAFNRNGTLFAYAVSYDWSKGYQ
FNTPNYPNMVKIHPTNDSETKQRPKK"
gene complement(<258533..>258823)
/gene="IGO2"
/locus_tag="AWJ20_2052"
/db_xref="GeneID:30033921"
mRNA complement(<258533..>258823)
/gene="IGO2"
/locus_tag="AWJ20_2052"
/product="Igo2p"
/transcript_id="XM_018878980.1"
/db_xref="GeneID:30033921"
CDS complement(258533..258823)
/gene="IGO2"
/locus_tag="AWJ20_2052"
/note="Protein required for initiation of G0 program;
prevents degradation of nutrient-regulated mRNAs via the
5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP
protein localizes to the cytoplasm and nucleus; IGO2 has a
paralog, IGO1, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14562095]; GO_function:
GO:0019208 - phosphatase regulator activity [Evidence IMP]
[PMID 23861665]; GO_process: GO:0048255 - mRNA
stabilization [Evidence IGI] [PMID 20471941]"
/codon_start=1
/product="Igo2p"
/protein_id="XP_018736939.1"
/db_xref="GeneID:30033921"
/translation="MYGRLPSRAELLNRKLKDRKFFDSGDYALKQAGKDTTQVVGSEH
PVPEIIPHHTSPPQSRHASFSYGSATAAHDPSLMAAHELDLKLKGIHRSSIA"
gene complement(<264441..>264833)
/gene="MRPS16"
/locus_tag="AWJ20_2053"
/db_xref="GeneID:30033922"
mRNA complement(<264441..>264833)
/gene="MRPS16"
/locus_tag="AWJ20_2053"
/product="mitochondrial 37S ribosomal protein MRPS16"
/transcript_id="XM_018878981.1"
/db_xref="GeneID:30033922"
CDS complement(264441..264833)
/gene="MRPS16"
/locus_tag="AWJ20_2053"
/inference="similar to AA sequence:KEGG_Orthology:K02959"
/note="Mitochondrial ribosomal protein of the small
subunit; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_component: GO:0005763 - mitochondrial
small ribosomal subunit [Evidence IPI] [PMID 12392552];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA,IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IEA]; GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IPI] [PMID 12392552]; GO_process:
GO:0032543 - mitochondrial translation [Evidence IC] [PMID
12392552]; GO_process: GO:0006412 - translation [Evidence
IEA]"
/codon_start=1
/product="mitochondrial 37S ribosomal protein MRPS16"
/protein_id="XP_018736940.1"
/db_xref="GeneID:30033922"
/translation="MKGSVRIRLARFGRKHAPVYNIVVAKAKSGRDKLPVEVIGTYNP
IATPLTPEQKERGEKPVKDIKIDFHRSKYWIGVGAQPTEVVARLFRKAGVLPPQWPGP
NSGPVVPKKNEIQGVKAITEPLPEPPVR"
gene complement(<265090..>265161)
/locus_tag="AWJ20_2054"
/db_xref="GeneID:30033923"
tRNA complement(<265090..>265161)
/locus_tag="AWJ20_2054"
/product="tRNA-Thr"
/db_xref="GeneID:30033923"
gene <266084..>268516
/gene="DNM1"
/locus_tag="AWJ20_2055"
/db_xref="GeneID:30033924"
mRNA <266084..>268516
/gene="DNM1"
/locus_tag="AWJ20_2055"
/product="dynamin-related GTPase DNM1"
/transcript_id="XM_018878982.1"
/db_xref="GeneID:30033924"
CDS 266084..268516
/gene="DNM1"
/locus_tag="AWJ20_2055"
/inference="similar to AA sequence:KEGG_Orthology:K17065"
/note="Dynamin-related GTPase involved in mitochondrial
organization; required for mitochondrial fission and
morphology; assembles on the cytoplasmic face of
mitochondrial tubules at sites at which division will
occur; also participates in endocytosis and regulating
peroxisome abundance; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005741 - mitochondrial
outer membrane [Evidence IEA,IEA]; GO_component:
GO:0005741 - mitochondrial outer membrane [Evidence IDA]
[PMID 11038182]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005777 -
peroxisome [Evidence IDA] [PMID 16968746]; GO_function:
GO:0005525 - GTP binding [Evidence IEA,IEA]; GO_function:
GO:0003924 - GTPase activity [Evidence IEA]; GO_function:
GO:0003924 - GTPase activity [Evidence IDA] [PMID
16186251]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0042802 - identical
protein binding [Evidence IDA] [PMID 16186251];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0042803 - protein homodimerization
activity [Evidence IDA] [PMID 16186251]; GO_process:
GO:0006184 - GTP catabolic process [Evidence IEA];
GO_process: GO:0001300 - chronological cell aging
[Evidence IMP] [PMID 18298044]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0000266 -
mitochondrial fission [Evidence IGI,IMP] [PMID 10559943];
GO_process: GO:0000001 - mitochondrion inheritance
[Evidence IGI] [PMID 17336903]; GO_process: GO:0051646 -
mitochondrion localization [Evidence IGI] [PMID 23341591];
GO_process: GO:0007005 - mitochondrion organization
[Evidence IMP] [PMID 9786946]; GO_process: GO:0016559 -
peroxisome fission [Evidence IGI] [PMID 18445678];
GO_process: GO:0007031 - peroxisome organization [Evidence
IGI,IMP] [PMID 16968746]; GO_process: GO:0051260 - protein
homooligomerization [Evidence IDA] [PMID 16186251]"
/codon_start=1
/product="dynamin-related GTPase DNM1"
/protein_id="XP_018736941.1"
/db_xref="GeneID:30033924"
/translation="MAALEDLIPVVNKLQDLVFNTIGSDTLDLPQVVVVGSQSSGKSS
VLENIVGRDFLPRGTGIVTRRPLILQLVNIPSSAPPTWTQNDNTGYGTNNVNNGSTSS
NSTTSNSLSSSPASTHGAIAGGGPNPNATFVREAEWGEFLHIPGRRFYDFSDIRREIE
SETARIAGNNKGINRLPINLKIFSPHVLNLTLVDLPGLTKIPIGDQPTDIERQTRGLI
SEYIAKPNSIILAVSPANVDLVNSESLKLARQVDPQGKRTIGVLTKLDLMDQGTNALD
ILSGHVYPLKLGFIGVVNRSQQDIQEGKSLDESLTAEKNFFATHPVYRNIAHKCGTAY
LAKSLNQTLMAHIRDRLPDIKARLNTLMGQTEQELASYGVDSSYSKENRGPLILQLMT
RFASSFIASIEGTSSEISTKELCGGARIYYIYNEVFGQSLTNIDPVGTLSHSDVRTAI
RNSTGPRPSLFVPELAFDLLVKPQIKLLEGPSQRCVELVYEELMKICHNCGSTELSRY
PKLQAKLIEVISDLLRERLGPTSAYVSSLIDIQRAYINTNHPNFVGAAAAMASVLEEK
QAKEKKVKELEKAAHEKKLLGDLAFNGSAATDGSDTPVSGQEPPADHILQNGSADQTK
KKHRPSSSSNPITNNVPATHEATPNGHKDSFLNYFFGKDGAEQSSVGGHSALSARSKD
SPFIQSPAASDPSSARFDLRDIESSLDNPLEHFSEREQLECELITRLIISYFDIVRES
IQDQVPKAVMHLLVNFSKESAQNRLVSELYKESLFDDLLYEDETLAQEREKCQNMLKT
YKEAARVIGEVV"
gene complement(<268605..>269843)
/gene="AIM17"
/locus_tag="AWJ20_2056"
/db_xref="GeneID:30033925"
mRNA complement(<268605..>269843)
/gene="AIM17"
/locus_tag="AWJ20_2056"
/product="Aim17p"
/transcript_id="XM_018878983.1"
/db_xref="GeneID:30033925"
CDS complement(268605..269843)
/gene="AIM17"
/locus_tag="AWJ20_2056"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; null mutant displays reduced
frequency of mitochondrial genome loss; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0051213 - dioxygenase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="Aim17p"
/protein_id="XP_018736942.1"
/db_xref="GeneID:30033925"
/translation="MIRGSRLLLRRQLARKYHVESFDKSGISLGGFRTAEPVKFRNLF
LRDACASTESVDVSTKQKTFSTAQLGDSIYAKNVTVNPSTQSLDVEWADGHKSSYSQD
FLETYSNTVSRRRYRHLDLHDTNIWNSGTSKFTYDDAYSTSYKAYLESPNDLVKSLQR
DGIAFITDIPPGEKFNSSKITVEHIAEQIGPIKNTFYGPSWNVISQSMAKNVAYTAVY
LPLHMDLLYFESPPGVQLLHVIENNTIGGESIFADSFAAALHILETDPDAYSALTEVP
ISYHYVNDNQHYYFARPLIVEDVNSSVDEKTGRRAISHINYSPPFQGPIDAIATTHDV
ADNVVTNFYRGLRLFEEYIENPSNQIEVKMAENTCVLFMNRRTLHGRQQFQPGSGNRW
FKGTYLDIDSFQSKLRTSKS"
gene complement(<271524..>272657)
/gene="RTT109"
/locus_tag="AWJ20_2057"
/db_xref="GeneID:30033926"
mRNA complement(<271524..>272657)
/gene="RTT109"
/locus_tag="AWJ20_2057"
/product="H3 histone acetyltransferase RTT109"
/transcript_id="XM_018878984.1"
/db_xref="GeneID:30033926"
CDS complement(271524..272657)
/gene="RTT109"
/locus_tag="AWJ20_2057"
/inference="similar to AA sequence:KEGG_Orthology:K11309"
/codon_start=1
/product="H3 histone acetyltransferase RTT109"
/protein_id="XP_018736943.1"
/db_xref="GeneID:30033926"
/translation="MSIETRFESELREFLPKFKEPFGLVHIRTNPRKCRRLVYLPKKQ
SRSVPLDASSTHFFVLSYKNIDFFALEIIIYESPNSMTLFVSKADTNGHYYELEAGAS
LSVLDITTGLLRGLLHHYIKPSKLLRICLFAKSEGQYLFPLSSENPNKHVLPDGELVK
WWLKVLNKLSGEFEQISRATLQIPGSDKSAIEAFFPKVTGNGGLPWTVGDIFRDESDL
KRKRQRGAVYYIPRFPDDPKGRFLDYLVLEKRAQTTSLDQFILELQSRQEFRLGSVVG
IIGVEGFIKENSDAYNLAVSSPHSVSYKEFVQLKDVLMNLSFATREFAESASEEFSDR
VPEFAKLKIFGAKEAKIKDKKTATTSAPVNVLGGMLIRKKPKK"
gene complement(<273377..>273655)
/gene="PRE3"
/locus_tag="AWJ20_2058"
/db_xref="GeneID:30033927"
mRNA complement(<273377..>273655)
/gene="PRE3"
/locus_tag="AWJ20_2058"
/product="proteasome core particle subunit beta 1"
/transcript_id="XM_018878985.1"
/db_xref="GeneID:30033927"
CDS complement(273377..273655)
/gene="PRE3"
/locus_tag="AWJ20_2058"
/inference="similar to AA sequence:KEGG_Orthology:K02738"
/note="Beta 1 subunit of the 20S proteasome; responsible
for cleavage after acidic residues in peptides;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005789 - endoplasmic reticulum membrane
[Evidence IC] [PMID 9087403]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IC] [PMID 9087403]; GO_component:
GO:0000502 - proteasome complex [Evidence IEA];
GO_component: GO:0005839 - proteasome core complex
[Evidence IEA]; GO_component: GO:0019774 - proteasome core
complex, beta-subunit complex [Evidence IDA] [PMID
9087403]; GO_component: GO:0034515 - proteasome storage
granule [Evidence IDA] [PMID 18504300]; GO_function:
GO:0004175 - endopeptidase activity [Evidence IEA];
GO_function: GO:0004175 - endopeptidase activity [Evidence
IMP] [PMID 9207060]; GO_function: GO:0004175 -
endopeptidase activity [Evidence IMP] [PMID 9312134];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0008233 - peptidase activity
[Evidence IEA]; GO_function: GO:0004298 - threonine-type
endopeptidase activity [Evidence IEA,IEA]; GO_process:
GO:0010499 - proteasomal ubiquitin-independent protein
catabolic process [Evidence IDA] [PMID 19162040];
GO_process: GO:0043161 - proteasome-mediated
ubiquitin-dependent protein catabolic process [Evidence
IDA] [PMID 11545745]; GO_process: GO:0043161 -
proteasome-mediated ubiquitin-dependent protein catabolic
process [Evidence IDA] [PMID 19029916]; GO_process:
GO:0006508 - proteolysis [Evidence IEA]; GO_process:
GO:0051603 - proteolysis involved in cellular protein
catabolic process [Evidence IEA]"
/codon_start=1
/product="proteasome core particle subunit beta 1"
/protein_id="XP_018736944.1"
/db_xref="GeneID:30033927"
/translation="MAVKFKDGIILGADSRTTTGAFIANRVTDKLTQVHDKVWCCRSG
SAADTQAVADIVHYHLQAYAAENNEPPSAKVAASIFRVLCYNNKDNLR"
gene <274720..>276381
/gene="moe1"
/locus_tag="AWJ20_2059"
/db_xref="GeneID:30033928"
mRNA <274720..>276381
/gene="moe1"
/locus_tag="AWJ20_2059"
/product="translation initiation factor eIF3d Moe1"
/transcript_id="XM_018878986.1"
/db_xref="GeneID:30033928"
CDS 274720..276381
/gene="moe1"
/locus_tag="AWJ20_2059"
/inference="similar to AA sequence:KEGG_Orthology:K03251"
/codon_start=1
/product="translation initiation factor eIF3d Moe1"
/protein_id="XP_018736945.1"
/db_xref="GeneID:30033928"
/translation="MSAPRLPKLASTSEPWGPPSEVPTSLRFNNVPYAPYSKGDKLGK
AADWAFDAGKDGKDQKRQQYGRGFRDPYHAYGSNFTSFFTNEEDEGITSFSVVDSAAK
AAPKNAGGRGGSQHTILKSRRGGAGAAGGRGGSFQSQRGHHQGQGRGGQGGGRGQGFK
RPGWRDYDKPQKVRDASVNITEGWSLIQSIGFNEFQKLSFDVRQGESIDSYGSVHYYN
NKTLDKPSVNAKLEPLDRIVYNISTSDDPVIQELSAKDAGTVFATDSIISLLMCVTKT
VNPWDIIITKKNGKVYLDKREGGPLDLVSVDENAADAPADATDSKDTLNNATALAYEA
TYINQNFNANAVIESSETYDFGKPNPFYSEEDQKQSEPLLAHGYDYKKFNLANNVEEE
PINLVIRTEIDAAQKNLNGSVSLVTVKALNEYGGSTGALEWKNRFVNARGAIVAAEMK
NNLCKISRWTVQSILAGANLMKIGFVSRVNPKDNTQHTIIGVIGQEPAQLSNQINLNL
NNGWGIVKSVVNICSELDEGKYVLLRDPNNPAVKLYKVPSNAFEE"
gene complement(<276531..>279335)
/locus_tag="AWJ20_2060"
/db_xref="GeneID:30033930"
mRNA complement(<276531..>279335)
/locus_tag="AWJ20_2060"
/product="centrin-binding protein Sfi1"
/transcript_id="XM_018878988.1"
/db_xref="GeneID:30033930"
CDS complement(276531..279335)
/locus_tag="AWJ20_2060"
/codon_start=1
/product="centrin-binding protein Sfi1"
/protein_id="XP_018736946.1"
/db_xref="GeneID:30033930"
/translation="MTSSHRANDDGSEIGQDAISTVDGLTYYDPGHLTSDEDPESFSN
SVLRAAARRLSNVNTSAGDEEDQESEEDFDDGDVLQQMKSPIQANRDHQPILPAPPIP
HDAIRKTKLTSSDVQLLRLVTEAADRRIRSNRSNSPIKDNGDSSLNEADFAELFQTYK
VFTARNKITPENNPSYYHCLNVIVHLCSYPQETWRERLEQLLTDFSSAVTRRLRYQDL
EQRLKKFQLYYQSRVLGLWYSRYVEQSMYDQNLQTVAAIRYRQTLKRDAFKVWKYNFE
VEQWSLSSAELRYEKSLLRKSLAKLYNKSSSIEGDRNIAKMFILGKFLSKWSKSYHYN
KQIGKSASLRYMSGLKRRIFYDWTFKFDEEMAEARANETIAIKCFNSWKNKTARFIQI
GLKTEEQADARLAERAIRQWSNLLADRSNDYSLATSVYKKNLAGTVLAVWKKRTQLSL
AAYPLLEKNWQSKASVVFGNWHLRTLQSLEAKRFRDFMLCHRILRSWRLQAGAVLIIK
GIDVNIVKQRFHCWLLLERLELVLRYRNNSIKLKVLKHWFIRSRRSFRHNWALHKEAV
SYDCARTQIKALFSWQRRLKSHRQDEQNAQGIYKQRKQKEYFSKYLAALFEYSQNYHL
ACKTYERSVVTRTLLEWRDEAYRRKFKRLAATYREQKRKFNHQRLKVVFVFWRSRYAE
VVNDQQLALSMITQRDLLVKKSTFHGWASKIDHFESELDRAIYTYNANLLSLAIEKWR
QKNTKIQQDNDMAGLRDMNQQWSLLGKVLRGWSYKVSERKRDYSLADDIYSKRQRSKF
GRIWKLWHAKYSEQTLMRQFGQTNIGFTELEENKSPTARFKNPTQNIPHTIARIPQGA
SGSPDTRHFLTSITKPSTLFNTSLLETPTRARASGINVPLTAVEQWRKMKSSSKLRNK
LSDSNESNEPNDPEMNLTDN"
gene <280181..>281587
/gene="OST1"
/locus_tag="AWJ20_2061"
/db_xref="GeneID:30033931"
mRNA <280181..>281587
/gene="OST1"
/locus_tag="AWJ20_2061"
/product="Ost1p"
/transcript_id="XM_018878989.1"
/db_xref="GeneID:30033931"
CDS 280181..281587
/gene="OST1"
/locus_tag="AWJ20_2061"
/inference="similar to AA sequence:KEGG_Orthology:K12666"
/note="Alpha subunit of the oligosaccharyltransferase
complex of the ER lumen; complex catalyzes
asparagine-linked glycosylation of newly synthesized
proteins; GO_component: GO:0005783 - endoplasmic reticulum
[Evidence IEA,IEA]; GO_component: GO:0005789 - endoplasmic
reticulum membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA,IEA]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0008250 - oligosaccharyltransferase
complex [Evidence IPI] [PMID 10358084]; GO_component:
GO:0008250 - oligosaccharyltransferase complex [Evidence
IPI] [PMID 16297388]; GO_component: GO:0008250 -
oligosaccharyltransferase complex [Evidence IPI] [PMID
9405463]; GO_function: GO:0004579 -
dolichyl-diphosphooligosaccharide-protein glycotransferase
activity [Evidence IEA]; GO_function: GO:0004579 -
dolichyl-diphosphooligosaccharide-protein glycotransferase
activity [Evidence IDA] [PMID 9015380]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_function: GO:0016757 - transferase activity,
transferring glycosyl groups [Evidence IEA]; GO_process:
GO:0006487 - protein N-linked glycosylation [Evidence IDA]
[PMID 9015380]; GO_process: GO:0018279 - protein N-linked
glycosylation via asparagine [Evidence IPI] [PMID
9988747]; GO_process: GO:0006486 - protein glycosylation
[Evidence IEA,IEA]"
/codon_start=1
/product="Ost1p"
/protein_id="XP_018736947.1"
/db_xref="GeneID:30033931"
/translation="MRLVNLLQVSVALFSVASAESIIPKTFENVQLTQSIDLTGSYLK
RSLDITARNIADEPQQVYIVAVDSKAFPNLSILEGQDKSTGRSVLARRIEDAHDDGVE
YYQLSLREPVQPGEELNLLVAEAYSGLLSPLPEVGGQDDEQYLVYSGSKYALSPYDTL
VSSLKIKIYGTYAEELTIGDDEPEQATVEGSGHILSFGEYKELAPLSSKPFKLRYSNP
RPLVRADKLERDIWVSHWGASVSIEETYWLTNIGTRLKDSFSRLDYNKGQSQYNLNVA
SIRQLRFPLGPNARDAYYTDLVGNVSTSHFRSNGQESILEIAPRYPVFGGWHYNFTVG
WSHDLSDFVKDVGNENYLLKIPIIQGPQDISYGEVDVRVILPEGASDIQVVSLFGAST
DISNTYSFLDTKGRPTVEFKYNNLIDGHRSGEFFVQYTYTTGDSLRKPLAIALTFASF
FTLFLVFDKIDVSIFRKK"
gene complement(<281858..>283507)
/locus_tag="AWJ20_2062"
/db_xref="GeneID:30033932"
mRNA complement(<281858..>283507)
/locus_tag="AWJ20_2062"
/product="hypothetical protein"
/transcript_id="XM_018878990.1"
/db_xref="GeneID:30033932"
CDS complement(281858..283507)
/locus_tag="AWJ20_2062"
/inference="similar to AA sequence:KEGG_Orthology:K02605"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736948.1"
/db_xref="GeneID:30033932"
/translation="MVSISSKECPNLKTALKLIVARLTESQSAQGGSAAEDNVADEEK
DEDLEGEDVPMGANDIRYDRRLRYDLDILAQWCNKQVRSEAVTSLDQLRIVISIQDAD
SFQVSILTGLIEMMQSYITTIPLKLMLNVATSLEVFQEKLPRKCIRLLRGKAFQAQMA
EGIMKILRETLFKYDESTLLLSPDLFNSLVHRQKQSLESVDTFISSLKYIFMAHYYSN
PFSVLTTGNEHVNGFITSQHLRALRMLPSFKTFVERKLEQGSYSEAAKLLEDDDYLKS
LISISIEEFREYARNVLSSIEIIIQLEQSLGYEESCEFRNTMDLYPAALSGELINLPK
GRRGTTFIDSLISKVRKLDDESEMPFIVDQLETICHDDKSGVAWDIYSRFQEKCHSAL
DASQPLNMANIFADTLETHFKFALVSYKEMFLYEVLLSDLFTLQENVFLPTYRGALEL
GLSNPKHYWGDVTESTAQAPHISVLYQLYRESSVFINIFDYYSVFKGLIHRPDNLADD
EWDRKSLAWFLQGIAELKFIGIIRDSKRKFECVEKIAWKDL"
gene complement(<285387..>285484)
/locus_tag="AWJ20_2063"
/db_xref="GeneID:30033933"
tRNA complement(<285387..>285484)
/locus_tag="AWJ20_2063"
/product="tRNA-Leu"
/db_xref="GeneID:30033933"
gene complement(<285954..>286562)
/gene="FRE2"
/locus_tag="AWJ20_2064"
/db_xref="GeneID:30033934"
mRNA complement(<285954..>286562)
/gene="FRE2"
/locus_tag="AWJ20_2064"
/product="Fre2p"
/transcript_id="XM_018878991.1"
/db_xref="GeneID:30033934"
CDS complement(285954..286562)
/gene="FRE2"
/locus_tag="AWJ20_2064"
/note="Ferric reductase and cupric reductase; reduces
siderophore-bound iron and oxidized copper prior to uptake
by transporters; expression induced by low iron levels but
not by low copper levels; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence IEA,IEA]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 8164662];
GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
activity [Evidence IEA]; GO_function: GO:0000293 -
ferric-chelate reductase activity [Evidence IDA] [PMID
8164662]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_process: GO:0015677 -
copper ion import [Evidence IDA] [PMID 9153234];
GO_process: GO:0006825 - copper ion transport [Evidence
IEA]; GO_process: GO:0006811 - ion transport [Evidence
IEA]; GO_process: GO:0055072 - iron ion homeostasis
[Evidence IEA]; GO_process: GO:0006826 - iron ion
transport [Evidence IDA] [PMID 8164662]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]"
/codon_start=1
/product="Fre2p"
/protein_id="XP_018736949.1"
/db_xref="GeneID:30033934"
/translation="MSILSEQENRTKTMPVMVEGFYKSFRQFSNYDNVMLVAGGTGIT
TAFSQVTSLLFQDRSRTIKLIWAVRSPAPLNWFSKEILYLRSWPKSIELQIYISQALF
ENDCGAKPCSPLDIEAGVQGVVGSQALDYGCGSNYQLAFITGGRPELQKEIANFIKHA
SGSIAICSCGPPTFIDRARYTFVHNMYKSDYHIDYFEEPYSC"
gene complement(<288477..>289481)
/locus_tag="AWJ20_2065"
/db_xref="GeneID:30033935"
mRNA complement(<288477..>289481)
/locus_tag="AWJ20_2065"
/product="hypothetical protein"
/transcript_id="XM_018878992.1"
/db_xref="GeneID:30033935"
CDS complement(288477..289481)
/locus_tag="AWJ20_2065"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736950.1"
/db_xref="GeneID:30033935"
/translation="MSSHVQQSIPPGLLSLLVTKYEEKSKKHAIQNLYRLQVESYKND
SGKYECPICQKSYVHFKHLRRHNRQHTGYRPHVCHICRDTFCRSDILKRHYERCFSKF
IATGKLSAKSRVPKVSKEYVQSANSPPLTDTPLPDQHLSTTDPVTLPGNMMGGTDTNS
KLNYTPTFYPAHPSPPSLDYIQSRDEFRQVVPRQPIYSSDNISIMPIDSPEYISSPGP
PNCPGAGPLIKQEELTPPAFESYSARDSCTSLVSPVSLDETVNSLPITGIVDDTPPTE
NEYFPRFVSNIRKPGQFYCEDTYGYRPDQTTIIANGPRIISVSSASNDIVLRWLEPDW
"
gene <292168..>293631
/locus_tag="AWJ20_2066"
/db_xref="GeneID:30033936"
mRNA <292168..>293631
/locus_tag="AWJ20_2066"
/product="hypothetical protein"
/transcript_id="XM_018878993.1"
/db_xref="GeneID:30033936"
CDS 292168..293631
/locus_tag="AWJ20_2066"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736951.1"
/db_xref="GeneID:30033936"
/translation="MAKNRKKNWQQEYGHEAGRLTRSKSQALKKILDTPGDRNNDDEF
PVLKRLSLYASSIKGDGNCLFRSFSDQYYGDNGDRHQEVRAEVVNYMKEHSDYFALFL
EGSGSGNESWPAYIKRMAKDGVYGDNLEIVAFARRYGVNVMIYQNDFMYVVSCQEDDK
EKNGVVRDIHIAYHTWEHYSSVRNLAGPHSGLPEVQPSLVEGGGAIEPSSGTSDDVPK
WMMDAIKRSIPYPPTEQMENLIVKLFRKYNNSHDFGRIVEEIIMMDNGDGEVNFDTDV
EVVDSLSDIDKPTTTSMIDQDKVTNDSSAKSIQKDQDTSVQVANSPETSMGVDEINSS
MSNSPEDSSSRSSSTSNTSSTTVASSVDDEGDESKGEAKLSNINGKRNSSLLSNELTE
ESESDRTHEISVIPRSRAKKDAVTNSAPRVKRLTAREKKERQKKEAAERKRASKISSV
SDRSRSTTAGLSDTSSSTTTATPSTTPSTPGIRTMYI"
gene complement(<294890..>295204)
/gene="TRX1"
/locus_tag="AWJ20_2067"
/db_xref="GeneID:30033937"
mRNA complement(<294890..>295204)
/gene="TRX1"
/locus_tag="AWJ20_2067"
/product="thioredoxin TRX1"
/transcript_id="XM_018878994.1"
/db_xref="GeneID:30033937"
CDS complement(294890..295204)
/gene="TRX1"
/locus_tag="AWJ20_2067"
/inference="similar to AA sequence:KEGG_Orthology:K03671"
/note="Cytoplasmic thioredoxin isoenzyme; part of
thioredoxin system which protects cells against oxidative
and reductive stress; forms LMA1 complex with Pbi2p; acts
as a cofactor for Tsa1p; required for ER-Golgi transport
and vacuole inheritance; with Trx2p, facilitates
mitochondrial import of small Tims Tim9p, Tim10p, Tim13p
by maintaining them in reduced form; abundance increases
iunder DNA replication stress; TRX1 has a paralog, TRX2,
that arose from the whole genome duplication;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
8603912]; GO_component: GO:0000324 - fungal-type vacuole
[Evidence IPI] [PMID 9657146]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005758 -
mitochondrial intermembrane space [Evidence IEA];
GO_component: GO:0005758 - mitochondrial intermembrane
space [Evidence IDA] [PMID 22984289]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_function:
GO:0015036 - disulfide oxidoreductase activity [Evidence
IDA] [PMID 19362171]; GO_function: GO:0015036 - disulfide
oxidoreductase activity [Evidence ISS] [PMID 1988444];
GO_function: GO:0015035 - protein disulfide oxidoreductase
activity [Evidence IEA]; GO_process: GO:0006888 - ER to
Golgi vesicle-mediated transport [Evidence IDA] [PMID
9813082]; GO_process: GO:0045454 - cell redox homeostasis
[Evidence IEA]; GO_process: GO:0045454 - cell redox
homeostasis [Evidence TAS] [PMID 11169096]; GO_process:
GO:0034599 - cellular response to oxidative stress
[Evidence ISS] [PMID 1988444]; GO_process: GO:0009263 -
deoxyribonucleotide biosynthetic process [Evidence IEA];
GO_process: GO:0006662 - glycerol ether metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]; GO_process:
GO:0080058 - protein deglutathionylation [Evidence
IDA,IGI] [PMID 20074363]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0006890 -
retrograde vesicle-mediated transport, Golgi to ER
[Evidence IDA] [PMID 9813082]; GO_process: GO:0042144 -
vacuole fusion, non-autophagic [Evidence IDA] [PMID
9657146]; GO_process: GO:0000011 - vacuole inheritance
[Evidence IMP] [PMID 8603912]; GO_process: GO:0000011 -
vacuole inheritance [Evidence IMP] [PMID 9015301]"
/codon_start=1
/product="thioredoxin TRX1"
/protein_id="XP_018736952.1"
/db_xref="GeneID:30033937"
/translation="MTVNPITSLSQFQEEIKFSGLTVIDFYATWCGPCKMISPVLDRI
SDATPSAHFFKVDVDAVPDVASENEISAMPTLLFFKNGVKVESVVGANIAKINAVVKD
LA"
gene <294927..>295127
/locus_tag="AWJ20_2068"
/db_xref="GeneID:30033938"
mRNA <294927..>295127
/locus_tag="AWJ20_2068"
/product="hypothetical protein"
/transcript_id="XM_018878995.1"
/db_xref="GeneID:30033938"
CDS 294927..295127
/locus_tag="AWJ20_2068"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736953.1"
/db_xref="GeneID:30033938"
/translation="MLAPTTDSTLTPFLKNNKVGMAEISFSEATSGTASTSTLKKWAE
GVASEILSRTGEIILQGPHQVA"
gene <296328..>296669
/locus_tag="AWJ20_2069"
/db_xref="GeneID:30033939"
mRNA <296328..>296669
/locus_tag="AWJ20_2069"
/product="hypothetical protein"
/transcript_id="XM_018878996.1"
/db_xref="GeneID:30033939"
CDS 296328..296669
/locus_tag="AWJ20_2069"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736954.1"
/db_xref="GeneID:30033939"
/translation="MGVESLTICSANLDKNVTKEHIYSLFLPFGPIVDVVPVTDPKHG
FRGSADIEFETEEDAAAAIDNMEGAQVFGKTLHVRLSRKDLSEGAPRVDMNKALWAQD
HEQDQPSTEPE"
gene complement(<301055..>302038)
/locus_tag="AWJ20_2070"
/db_xref="GeneID:30033941"
mRNA complement(<301055..>302038)
/locus_tag="AWJ20_2070"
/product="hypothetical protein"
/transcript_id="XM_018878998.1"
/db_xref="GeneID:30033941"
CDS complement(301055..302038)
/locus_tag="AWJ20_2070"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736955.1"
/db_xref="GeneID:30033941"
/translation="MASGAKYRPQRWRREARGLEDSFSKLNIEGESHAEADAKARARR
ERFSQPAKKEEYGLISRGEDNRLRVDSKARRTYFYQTQLRALKIIAASTSQRQYIEDV
LKIVDHEVSDSYQAVDTNGRHQSSDTDSEDSVLMSLRKLREALLPIEPDKFTKQVFLF
SVRMAVLAGKYQAFIPSVLYILDVIHPVVPLSFDEFRQVALLHVYHTAHFSNDVGTAY
KTLYHYLPDDKRAWTVLQALASNNFVSWVQQKRTEPDLWLRLSLDQNHATMAKNAIPT
ISKSYMKLHESSIERLLGLDWNTLTSNPNIGCVWPKDSTGTATIRQRKTQS"
gene <303610..>305703
/gene="TRP5"
/locus_tag="AWJ20_2071"
/db_xref="GeneID:30033942"
mRNA <303610..>305703
/gene="TRP5"
/locus_tag="AWJ20_2071"
/product="tryptophan synthase TRP5"
/transcript_id="XM_018878999.1"
/db_xref="GeneID:30033942"
CDS 303610..305703
/gene="TRP5"
/locus_tag="AWJ20_2071"
/inference="similar to AA sequence:KEGG_Orthology:K01694"
/note="Tryptophan synthase; catalyzes the last step of
tryptophan biosynthesis; regulated by the general control
system of amino acid biosynthesis; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016829 - lyase
activity [Evidence IEA]; GO_function: GO:0004834 -
tryptophan synthase activity [Evidence IEA,IEA];
GO_function: GO:0004834 - tryptophan synthase activity
[Evidence IDA] [PMID 391563]; GO_function: GO:0004834 -
tryptophan synthase activity [Evidence ISO] [PMID
6276387]; GO_function: GO:0004834 - tryptophan synthase
activity [Evidence IDA] [PMID 6342605]; GO_process:
GO:0009073 - aromatic amino acid family biosynthetic
process [Evidence IEA]; GO_process: GO:0008652 - cellular
amino acid biosynthetic process [Evidence IEA];
GO_process: GO:0008152 - metabolic process [Evidence IEA];
GO_process: GO:0000162 - tryptophan biosynthetic process
[Evidence IEA,IEA,IEA]; GO_process: GO:0000162 -
tryptophan biosynthetic process [Evidence IMP] [PMID
10790693]; GO_process: GO:0006568 - tryptophan metabolic
process [Evidence IEA]"
/codon_start=1
/product="tryptophan synthase TRP5"
/protein_id="XP_018736956.1"
/db_xref="GeneID:30033942"
/translation="MSANLNATFAKCKKEGRNAMLTFLTAGFPSVAETVPLLKSLQDG
GADIIELGVPFSDPIADGPTIQKANQIALENGVSVHVCLELVRQARAAGVNIPIILMG
YYNPILQYGENKFVEDAKKAGVDGFLLVDLPPEEASRFRKISSNSGLSYIPLIAPSTT
NERLAQLSEIADSFLYVVSRMGTTGVAGGLDSNVSTLLKRVRQFTGEKPLAVGFGIST
RENYLTVSPVADGIVIGSYLISLIDNTPKEERLETVKKYINGIVGGREVVVDPRPESP
STTPVLSEDEEEQKKQFYALVDRFGDYGGQYVPEVLHQCLSELEKGFNEAIHDEKFWE
ELRSYYPYMGRPSNLYFADRLSEKVGGAKIWLKREDLNHTGSHKINNALAQVLIARRL
GKTQVIAETGAGQHGVATATVAAKFGLKCKVFMGAEDCRRQALNVFRMKLLGAEVVPV
HAGTKTLRDACSEALRTWVTTIGDTHYVLGSATGPHPFPTMVRTFQSVIGQETKEQFA
KYNDGKLPDVVVACVGGGSNCSGMFAPFIEDKSVKLVGVEAGGEGLQTDKHSATLSAG
KSGVLHGAKTYVLQNADGQVQDTHSISAGLDYPGVGPELASWKDSGRVKFIAATDLEA
LQGFRILSETEGIIPAMESSHAIHGAVELAKTMSPDEHVVICLSGRGDKDVQNIADLL
PTYGPKLGWDVRFEN"
gene <305901..>308699
/locus_tag="AWJ20_2072"
/db_xref="GeneID:30033943"
mRNA <305901..>308699
/locus_tag="AWJ20_2072"
/product="hypothetical protein"
/transcript_id="XM_018879000.1"
/db_xref="GeneID:30033943"
CDS 305901..308699
/locus_tag="AWJ20_2072"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736957.1"
/db_xref="GeneID:30033943"
/translation="MWPSWRSQGPKLGYGHDGFGIYVPHLVPETPWLFARRHDLGYKF
QSTGILKTYDASQDYRVINKKVDMDIIDDVKRRGQRLGELNPLQGDYIPNDLLTSYNS
ESYGFLHRTKHDPLKGSCIAIGSIRETVSSEGADSKTESDLQESAEEYSDEEESDFNS
RQAIAYVNGKDQYQVKFGFIDKASSEFSSANGSSYYANMPDINVATSFNVNSPVMQVE
FAEPEPEISPYMLVRSKNGTKILSVTSENDKIAVYDLLDIPFQSKQAYNFETLSRPVE
HCHSTFNPWNYANVATINSQGVWSLWDLSSEQSLVSLSSPTTMSAFNRHSQTSSPWNK
IIWGADSSSLYSINRKYLQLFDIRSDSATSSKGDTVLECTDRETHFRDMARSKINDNQ
FFVLDSKSVKWMDMRNPGSVLLSWDHFLDLEDTTIKLTVNGIKDNVHVVGVYSQIHQA
NFLYQFVESEGLPSSADDPILIVSPPDELAQSLELIPFTAEGNDGDIEMINLVRLPVD
GALQGEILTSDPDYQSENHTNNIDNGINSDIVNSANGVVKANKNTEYIESISVETDDV
RYKFDYILPDLVPVSKLDFRAVYEFLFENLSQIPEETSKDEDIENFARHLGETINSMF
IAKQSTSKSTGMNEDPDIRKRRTLTLLELSEIGTPLLRLYNNLEDLSALIHQLTDHYK
DTKISVKELRRDMNTLFGGEFVNNIQNNYDYLLSIWTNSLNTQTLGPERVAELTAQRK
KLALYIIIRLTLTSVGVYVEGESKPILQDTHLLKPLSKYTQVRNPHDFNLTANTELLL
DEWELGTNVDDYKWYRLGDTPMEDDTSSSVPDRYQQIMSQLSQHSQRMISPQPVRVRS
SRAKTTASSQPVGSNPFSSPIQSTTRLATSSRSSSQRVAPLQSSQSNMQRMAQSQPIQ
GESSQRRLKRKKRNEGFG"
gene <309160..>311664
/gene="CWH41"
/locus_tag="AWJ20_2073"
/db_xref="GeneID:30033944"
mRNA <309160..>311664
/gene="CWH41"
/locus_tag="AWJ20_2073"
/product="Cwh41p"
/transcript_id="XM_018879001.1"
/db_xref="GeneID:30033944"
CDS 309160..311664
/gene="CWH41"
/locus_tag="AWJ20_2073"
/inference="similar to AA sequence:KEGG_Orthology:K01228"
/note="Processing alpha glucosidase I; ER type II integral
membrane N-glycoprotein involved in assembly of cell wall
beta 1,6 glucan and asparagine-linked protein
glycosylation; also involved in ER protein quality control
and sensing of ER stress; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005789 - endoplasmic reticulum membrane [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IDA] [PMID 8576053]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence IEA];
GO_function: GO:0003824 - catalytic activity [Evidence
IEA]; GO_function: GO:0016787 - hydrolase activity
[Evidence IEA]; GO_function: GO:0016798 - hydrolase
activity, acting on glycosyl bonds [Evidence IEA];
GO_function: GO:0004573 - mannosyl-oligosaccharide
glucosidase activity [Evidence IEA,IEA]; GO_function:
GO:0004573 - mannosyl-oligosaccharide glucosidase activity
[Evidence IDA] [PMID 14680956]; GO_function: GO:0004573 -
mannosyl-oligosaccharide glucosidase activity [Evidence
IMP,ISS] [PMID 9430631]; GO_process: GO:0009272 -
fungal-type cell wall biogenesis [Evidence IMP] [PMID
9430631]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0009311 - oligosaccharide
metabolic process [Evidence IEA]; GO_process: GO:0006487 -
protein N-linked glycosylation [Evidence IMP] [PMID
9363442]"
/codon_start=1
/product="Cwh41p"
/protein_id="XP_018736958.1"
/db_xref="GeneID:30033944"
/translation="MNCQSRRRPSPYNWSQFNHKLKMVHWPHFLSLANLIALSWLCVV
FVSSSPQSVLKSAPLINEYARQSNSSLLWGPYRSNLYFGIRPRIPKSLLSGLMWYNAD
SYDGLKNLRHTCEQGDAMAGYGWQQYDPRSGGHQVINDLDNFVNISTDFVKTEVGSWA
VRVKGVPQNEQRRTTVIFYSGLEGHGKLDLESQLTPEGIEGPVELVGSSPELGDFSIE
VTTGPSTNRHPKTSHVVDKVRPSQNSHYASLMMPDDNVWLAKEVFQTFIEDNVSQFSA
LFDEADMIPPWSVFTLQNRDDLKGNVHMIQRTYQGSFEFDILYNPIEISESSKISSRK
LGDLLELSLSEFDTKFKRAFQFQPPFDSSLKHELFAKEMFSNLVGGIGYFYGTSIVDR
SLSDLDDDEGDWEVEAAALASGVGKEEGPFELFTAVPSRPFFPRGFYWDEGFHLIPVL
DYDADLTLEVLKSWFSLVDGDGWIAREQILGPEARSKVPTEFRTQYPHYANPPTLMLL
FSNIAKRAHEWSQSEHGTNFDGSLVLGNAHIEYPELLFSYAEKIYPELQSHYEWFRRT
QKGEIRQWDREAFSSKEGYRWRGRTPNHCLTSGLDDYPRAAMPHPGELHVDLLSWIGM
MTRSIKEIARVLGKDDDVEMYSEIEEAIVKNLYDLHWDHKEQMYCDSTIDDYEESVHV
CHRGYVSLFPFMLQLIPEDSTEQLLAAINDIKDPGGLWSPYGIRSLSKSDEFFGTKEN
YWRGPIWINMNYMILESLRYYGTSETSSAEVKSLSSEIYKELRVNIVNNVFNRWEETG
FAWEQYDSNTGKAQGVKHFLGWTSLVIKIMAMPEEL"
gene complement(<311800..>312810)
/gene="RTC2"
/locus_tag="AWJ20_2074"
/db_xref="GeneID:30033945"
mRNA complement(<311800..>312810)
/gene="RTC2"
/locus_tag="AWJ20_2074"
/product="Rtc2p"
/transcript_id="XM_018879002.1"
/db_xref="GeneID:30033945"
CDS complement(311800..312810)
/gene="RTC2"
/locus_tag="AWJ20_2074"
/note="Putative vacuolar membrane transporter for cationic
amino acids; likely contributes to amino acid homeostasis
by exporting cationic amino acids from the vacuole;
positive regulation by Lys14p suggests that lysine may be
the primary substrate; member of the PQ-loop family, with
seven transmembrane domains; similar to mammalian PQLC2
vacuolar transporter; RTC2 has a paralog, YPQ1, that arose
from the whole genome duplication; GO_component:
GO:0071627 - integral component of fungal-type vacuolar
membrane [Evidence IDA] [PMID 23169667]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0031966 - mitochondrial membrane [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005774 - vacuolar
membrane [Evidence IEA]; GO_component: GO:0005773 -
vacuole [Evidence IEA]; GO_function: GO:0015326 - cationic
amino acid transmembrane transporter activity [Evidence
IMP,ISA] [PMID 23169667]; GO_process: GO:0006865 - amino
acid transport [Evidence IEA]; GO_process: GO:0034488 -
basic amino acid transmembrane export from vacuole
[Evidence IMP,ISA] [PMID 23169667]; GO_process: GO:0006810
- transport [Evidence IEA]"
/codon_start=1
/product="Rtc2p"
/protein_id="XP_018736959.1"
/db_xref="GeneID:30033945"
/translation="MLENIDPPSHLRVVLSGMAGSSSLACWIVLLLPQLIEQWRLKSA
DGISIGFLIIWLLGDVTNLAGSLWANLRPEVILLAVWYCIADSLIMVSYFYYKRLARL
RQARKAHKHKQHRPSSGDDHEEDPTAPLLDRRNSANRKRPSTSAINEDDEVNTNGPSH
HEHHEHRHHHHHNQTRRDSLTSIVLETSSSSGIFSKVVLPVLFVIASGILGYLVSGNQ
SDNTGEISDEPLALGPQILGYTSAVLYLSARIPQIIQNHQRRSTHGLSLVFFVFSVLG
NATYAAGILLYRTDIQWVTLYFPWLLGSLGTILEDCVIFLQFYMYDSEHKDEDSESAV
HD"
gene <314581..>315369
/gene="RPL8B"
/locus_tag="AWJ20_2075"
/db_xref="GeneID:30033946"
mRNA <314581..>315369
/gene="RPL8B"
/locus_tag="AWJ20_2075"
/product="ribosomal 60S subunit protein L8B"
/transcript_id="XM_018879003.1"
/db_xref="GeneID:30033946"
CDS 314581..315369
/gene="RPL8B"
/locus_tag="AWJ20_2075"
/inference="similar to AA sequence:KEGG_Orthology:K02936"
/note="Ribosomal 60S subunit protein L8B; required for
processing of 27SA3 pre-rRNA to 27SB pre-rRNA during
assembly of large ribosomal subunit; depletion leads to a
turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8
S rRNAs; mutation results in decreased amounts of free 60S
subunits; homologous to mammalian ribosomal protein L7A,
no bacterial homolog; RPL8B has a paralog, RPL8A, that
arose from the whole genome duplication; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0022625 - cytosolic large ribosomal subunit [Evidence
IDA] [PMID 11983894]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA,IEA];
GO_component: GO:0005840 - ribosome [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IC] [PMID 11983894]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence IC] [PMID
11983894]; GO_process: GO:0000470 - maturation of LSU-rRNA
[Evidence IMP] [PMID 22893726]; GO_process: GO:0042254 -
ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="ribosomal 60S subunit protein L8B"
/protein_id="XP_018736960.1"
/db_xref="GeneID:30033946"
/translation="MTQPAKGKKPAPAPFSTKTASTKAAKNPLIEKRPRNFGIGQDIQ
PKRNLSRFVKWPEYVRLQRQKKILNLRLKVPPAIAQFQNTLDKNTAAQAFKIFNKYRP
ETAAEKKERLTKEAAAIAEGKKKEDVSEKPYVVKYGLNHVVSLIENKKPQLVLIANDV
DPIELVVFLPALCRKMGVPYAIIKGKARLGTLVHKKTAAVVALTEVKSDDKAALSTLL
STIEANYSSKYEELRRKWGGGIMGAKAQAKIEKRRKILEAAVKV"
gene <315607..>317217
/locus_tag="AWJ20_2076"
/db_xref="GeneID:30033947"
mRNA <315607..>317217
/locus_tag="AWJ20_2076"
/product="putative membrane protein"
/transcript_id="XM_018879004.1"
/db_xref="GeneID:30033947"
CDS 315607..317217
/locus_tag="AWJ20_2076"
/note="similar to Pantoea sp. aB, conserved hypothetical
protein (NCBI: ZP_07377486.1)"
/codon_start=1
/product="putative membrane protein"
/protein_id="XP_018736961.1"
/db_xref="GeneID:30033947"
/translation="MSSSLVPDRTPGSMSQYSKINLVSYLLLCLFVISFLVFLNSTQP
FVLTDILGDDVNIGDKVGSLAFADEIVSIVLCPLWGSLSDKVGTRPVSVIGLLILSTS
LFFYTTGNSVYPDLLLLRMFFAVGTSACVSMIPAILAELSASYEESKFDGYRQVEDMN
WGDDVSNDEENDALEAIEERNAYFDDPTVVSRDTPFFEQSEASPKSYRNAKLAGLVGV
VTGIGAVLSVSLFLPLPTWLDRNSGGENDAKIALRNSYYIVSAIAFFVSILLFLGLHK
DRSKSFRLWLNGGSVSIFDEAALDTMPDSLPYFQTIKMGFLAAKDRQVALSYVGGLVA
RSATVALTLFVPLAVNFYFYEQGICKVGDPHDALPDVKKGCHEAYVLAAILTGIANTV
SLVFAPIWGLLGDYIGQHGSLMVSALVGFTGCIGFAFVEKPNSPVSFVMACFLGAGQI
GAIVLSMSMCTNSRRQYNGSISGVYSLSGGLGILLITKVGGYISDIWKGAPFVILAGF
YFLLCIAIIWALPKGESKGFSRLFSPPR"
gene <317638..>317724
/locus_tag="AWJ20_2077"
/db_xref="GeneID:30033948"
tRNA <317638..>317724
/locus_tag="AWJ20_2077"
/product="tRNA-Tyr"
/db_xref="GeneID:30033948"
gene <318406..>319170
/gene="RPL2A"
/locus_tag="AWJ20_2078"
/db_xref="GeneID:30033949"
mRNA <318406..>319170
/gene="RPL2A"
/locus_tag="AWJ20_2078"
/product="ribosomal 60S subunit protein L2A"
/transcript_id="XM_018879005.1"
/db_xref="GeneID:30033949"
CDS 318406..319170
/gene="RPL2A"
/locus_tag="AWJ20_2078"
/inference="similar to AA sequence:KEGG_Orthology:K02938"
/note="Ribosomal 60S subunit protein L2A; homologous to
mammalian ribosomal protein L2 and bacterial L2; RPL2A has
a paralog, RPL2B, that arose from the whole genome
duplication; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0022625 - cytosolic
large ribosomal subunit [Evidence IDA] [PMID 18782943];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0030687 - preribosome, large subunit
precursor [Evidence IDA] [PMID 23212245]; GO_component:
GO:0030529 - ribonucleoprotein complex [Evidence IEA];
GO_component: GO:0005840 - ribosome [Evidence IEA,IEA];
GO_function: GO:0003723 - RNA binding [Evidence IEA];
GO_function: GO:0019843 - rRNA binding [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IEA]; GO_function: GO:0003735 -
structural constituent of ribosome [Evidence IDA] [PMID
18782943]; GO_process: GO:0002181 - cytoplasmic
translation [Evidence IDA] [PMID 18782943]; GO_process:
GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="ribosomal 60S subunit protein L2A"
/protein_id="XP_018736962.1"
/db_xref="GeneID:30033949"
/translation="MGRVIRNQRKGAGSIFTSHTRLRKGAAKLRTLDFAERHGYIRGV
VKKILHDPGRGAPLATVAFRDPYKYKVREETFIANEGVYTGQFIYAGKKASLNVGNVL
PLGSVPEGTILSNVEEKVGDRGALGRTSGNYVIVIGHNPEEGKTRLKLPSGAKKIVSS
QARGVVGVVAGGGRIDKPLLKAGNAFHKFKVKRNSWPKTRGVAMNPVDHPHGGGNHQH
IGKASTISRGAVAGQKAGLIAARRTGLLRGTQKTKD"
gene <319897..>323022
/gene="SMC2"
/locus_tag="AWJ20_2079"
/db_xref="GeneID:30033950"
mRNA <319897..>323022
/gene="SMC2"
/locus_tag="AWJ20_2079"
/product="condensin subunit SMC2"
/transcript_id="XM_018879006.1"
/db_xref="GeneID:30033950"
CDS 319897..323022
/gene="SMC2"
/locus_tag="AWJ20_2079"
/inference="similar to AA sequence:KEGG_Orthology:K06674"
/note="Subunit of the condensin complex; condensin
reorganizes chromosomes during both mitosis and meiosis;
essential SMC chromosomal ATPase family member that forms
a subcomplex with Smc2p that has ATP-hydrolyzing and
DNA-binding activity, but other condensin subunits are
required for chromatin binding; required for clustering of
tRNA genes at the nucleolus; GO_component: GO:0005694 -
chromosome [Evidence IEA,IEA,IEA]; GO_component:
GO:0000796 - condensin complex [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0000799 -
nuclear condensin complex [Evidence IDA] [PMID 10811823];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0003680 - AT DNA binding [Evidence IDA]
[PMID 9727028]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IDA] [PMID 9727028]; GO_function: GO:0016887 -
ATPase activity [Evidence IDA] [PMID 12719426];
GO_function: GO:0000217 - DNA secondary structure binding
[Evidence IDA] [PMID 9727028]; GO_function: GO:0003682 -
chromatin binding [Evidence IDA] [PMID 16055730];
GO_function: GO:0003690 - double-stranded DNA binding
[Evidence IDA] [PMID 9727028]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006310
- DNA recombination [Evidence IEA]; GO_process: GO:0006281
- DNA repair [Evidence IEA]; GO_process: GO:0007049 - cell
cycle [Evidence IEA]; GO_process: GO:0051301 - cell
division [Evidence IEA]; GO_process: GO:0030261 -
chromosome condensation [Evidence IEA]; GO_process:
GO:0051276 - chromosome organization [Evidence IEA];
GO_process: GO:0010032 - meiotic chromosome condensation
[Evidence IC] [PMID 10811823]; GO_process: GO:0051307 -
meiotic chromosome separation [Evidence IC] [PMID
10811823]; GO_process: GO:0007076 - mitotic chromosome
condensation [Evidence IEA]; GO_process: GO:0007076 -
mitotic chromosome condensation [Evidence IMP] [PMID
10811823]; GO_process: GO:0007067 - mitotic nuclear
division [Evidence IEA]; GO_process: GO:0000070 - mitotic
sister chromatid segregation [Evidence IMP] [PMID
10811823]; GO_process: GO:0070550 - rDNA condensation
[Evidence IMP] [PMID 10811823]; GO_process: GO:0007130 -
synaptonemal complex assembly [Evidence IC] [PMID
10811823]; GO_process: GO:0070058 - tRNA gene clustering
[Evidence IMP] [PMID 18708579]"
/codon_start=1
/product="condensin subunit SMC2"
/protein_id="XP_018736963.1"
/db_xref="GeneID:30033950"
/translation="MKVEEIIIDGFKSYATRTVISGWDASFNCITGLNGSGKSNILDA
ICFVLGITTMTTVRAQNLQDLIYKRGQAGVTRASVTILFDNSDKSNSPLGFEDQAQFS
VTRQIVLGGTSKFLINGHRAQQQSVQQLFQSVQLNINNPNFLIMQGQITKVLNMKPAE
ILALIEEAAGTRMFEDRREKAIKTMTKKEKKVEEILNLLHDEIEPKLENLRNEKRTFI
EYQQTLSSLERIEKMVVAHDYLKGSEKLLAHSQALEERKELLGNISASVDKLNSEIQN
LEEELKRLRKTKEDQISKGGKFQALEKQYKGACQEVARIRTLLELKDSTLKEENEKRD
ELKSTLAEHESRLKKQSSQYSTIEKEYETVKAKHVRDNEEISRNEELLQSLQTGVSSK
AGQETGYASQLQEVRTIVNNAKTTAAQAKVKQEHVEKLIKEDSAKVTKAQKQNQEMLN
TLAGAEEYYNSLKSKLSQAGWDPETIKTLKTKEYELGEQIRSLDDEIESMRRKTSNID
FHYSKPSPTFRASSVRGLVAQLFSLDKSKYPQATAIEVCAGGRLFNVVVDNDVTAAQL
LQNGKLKRRVTIIPLNKISSSRLSEERLSNAKKLAPGKVDLALNIVGYEADVFAAMEY
VFGSTLICKDTATAQLVTFDPKVRAKSVTLDGDIYDPMGTLSGGSKSNSSGLLISLQQ
YNELSRLLKEKNEDLANVKEEIARENKLMAQFKDIKKELDLKEHEITLYKQQLESSPS
ALVLAKHEERVKELEELSLQISDANKQFEEGGIELSRIEKDMNEFKQNKGSKLGQLKA
KVEKLRASYTQSEQVVKQKLEVFQTAQVEIEQLTDDVSTMKEQLEDKTNSIQDLAASI
ESINAQLKRAAQTESTYLQQLEAEKSALTGMDDELNTMEEIRDKKRKLVTEQGLESQK
LRHEIENLSKEHESLQHAISGLDQKYEWIQDQKQHFGKIGTPYDFSHANVDQSRKQLR
QLQERINGMKKTVNSKVMNMIESVEKKEESLKSMIQTIEKDKKKIEDTIVSLDNYKRE
ALIKTWEKVSV"
gene complement(<323724..>324818)
/gene="QCR6"
/locus_tag="AWJ20_2080"
/db_xref="GeneID:30033952"
mRNA complement(join(<323724..324203,324747..>324818))
/gene="QCR6"
/locus_tag="AWJ20_2080"
/product="ubiquinol--cytochrome-c reductase subunit 6"
/transcript_id="XM_018879008.1"
/db_xref="GeneID:30033952"
CDS complement(join(323724..324203,324747..324818))
/gene="QCR6"
/locus_tag="AWJ20_2080"
/inference="similar to AA sequence:KEGG_Orthology:K00416"
/note="Subunit 6 of the ubiquinol cytochrome-c reductase
complex; the complex, also known as the cytochrome bc(1)
complex or Complex III, is a component of the
mitochondrial inner membrane electron transport chain;
highly acidic protein; required for maturation of
cytochrome c1; may be loosely associated with the complex
since it is easily released into the intermembrane space;
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0005758 - mitochondrial intermembrane
space [Evidence IDA] [PMID 22984289]; GO_component:
GO:0005750 - mitochondrial respiratory chain complex III
[Evidence IDA] [PMID 10873857]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 11914276];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0070469 -
respiratory chain [Evidence IEA]; GO_function: GO:0008121
- ubiquinol-cytochrome-c reductase activity [Evidence
IEA]; GO_function: GO:0008121 - ubiquinol-cytochrome-c
reductase activity [Evidence IMP] [PMID 8288589];
GO_process: GO:0009060 - aerobic respiration [Evidence
IMP] [PMID 8288589]; GO_process: GO:1902600 - hydrogen ion
transmembrane transport [Evidence IEA]; GO_process:
GO:0006122 - mitochondrial electron transport, ubiquinol
to cytochrome c [Evidence IEA]; GO_process: GO:0006122 -
mitochondrial electron transport, ubiquinol to cytochrome
c [Evidence IMP] [PMID 8288589]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase subunit 6"
/protein_id="XP_018736964.1"
/db_xref="GeneID:30033952"
/translation="MSGITSFWSDLVESIMPTVAYAEEAPKQDAPLDHDSEEVESESK
SETETVAKNTGKESSEEETTEESSEESTEESTEESTEESTEEGSEEESEDGEEDDEEE
EEEEEEDEPEDILPELREKAAEGPCHSFKHHFEECIERVTAEQQEEGYAEKEYKEDCV
EEFFHLSHCIDEQVAPVLFTKLK"
gene complement(<326465..>327412)
/locus_tag="AWJ20_2081"
/db_xref="GeneID:30033953"
mRNA complement(<326465..>327412)
/locus_tag="AWJ20_2081"
/product="hypothetical protein"
/transcript_id="XM_018879009.1"
/db_xref="GeneID:30033953"
CDS complement(326465..327412)
/locus_tag="AWJ20_2081"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736965.1"
/db_xref="GeneID:30033953"
/translation="MSADTETPVEKVEEPTSTPSTTTPANDEPTPSSNGEAAVEYDTK
RVYIGNLPFRSTHKDLRDLFEDFHISSLNIPKSKVTHSSGRVFFKKLGYAFAEFPTEE
DAKKAVESVNEKVLNDRQLNVRFARVLDRKARKEKSAAAAAAAASTAETNGNDQSSTT
TTSSKEGGTSKAAPKKPAPRRRRPTGPDSTDTVFLGNLPVDTTVEELEEFFKPLSPVE
VKLAIKKPRKAKRKDAVITLPGRTLGFVKLADEATQKKAIDEYNGKTFKDVELQVKVA
TQLVEEEAEEEGAADVSSADATDADATSEAASAPVSAEN"
gene <331036..>332076
/locus_tag="AWJ20_2082"
/db_xref="GeneID:30033954"
mRNA <331036..>332076
/locus_tag="AWJ20_2082"
/product="hypothetical protein"
/transcript_id="XM_018879010.1"
/db_xref="GeneID:30033954"
CDS 331036..332076
/locus_tag="AWJ20_2082"
/inference="similar to AA sequence:KEGG_Orthology:K14684"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736966.1"
/db_xref="GeneID:30033954"
/translation="MATVKEVGKSATSPSSLASSSSSPPSSLADNPSVIALRNYLALP
IVSSFVAGGVAGAVSRTVVSPLERMKIIFQVQGPGSQSYQGVGPTLAKMWREEGWRGF
MRGNGINCVRIVPYSAVQFSSYTIYKNFLLSRTPSSKRVDLDTLERLGAGALAGITSV
VATYPLDIVRTRLSIQTASLKPLKVSGSQVHAQKPLGMWSVMSDMYKTEGGFRALYRG
LIPTTMGVAPYVGLNFAVYESMREYVTPKGEKNPSALGKLFAGAVSGAVAQTITYPFD
VLRRRFQVVSMGAGQLGFQYTSVWDAISSIVKQEGIRGIYKGLSANLLKVAPSMASSW
LSYEYTKELLKY"
gene complement(<332385..>333455)
/locus_tag="AWJ20_2083"
/db_xref="GeneID:30033955"
mRNA complement(<332385..>333455)
/locus_tag="AWJ20_2083"
/product="hypothetical protein"
/transcript_id="XM_018879011.1"
/db_xref="GeneID:30033955"
CDS complement(332385..333455)
/locus_tag="AWJ20_2083"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736967.1"
/db_xref="GeneID:30033955"
/translation="MSFIGSGSTLGFAALVKCAALNFPANRGVATSVPVAAFGLSAFF
LSGVASIISPGDTYGLLCILSFLPMFLFIITLPFIKVLPLEYVSANVRTSSEKGSLCI
NLPVISEKEHSEPSSSPRRLYSGSHALTDSPIDIYGTRLLRTKTFWLHFFLLGTLAGV
GQMYIYSCGYCVRALIEDPESNAHALQKLQSVQVGTISLTSFSGRIMSGFLSDYIHNK
HHLPRTLILIASASIMLVAQASAFSVTSASSLWIPSAATGLAYGICYGSYPTIVSDTF
GMRYFSQNWGFLALSPVPTSYILNMMFGALYDVNSTVDEAGHRVCALGKDCYSRSFII
TSGAITTALLVLVPYVMTRKRR"
gene <334892..>336025
/gene="FMT1"
/locus_tag="AWJ20_2084"
/db_xref="GeneID:30033956"
mRNA <334892..>336025
/gene="FMT1"
/locus_tag="AWJ20_2084"
/product="Fmt1p"
/transcript_id="XM_018879012.1"
/db_xref="GeneID:30033956"
CDS 334892..336025
/gene="FMT1"
/locus_tag="AWJ20_2084"
/inference="similar to AA sequence:KEGG_Orthology:K00604"
/note="Methionyl-tRNA formyltransferase; catalyzes the
formylation of initiator Met-tRNA in mitochondria;
potential Cdc28p substrate; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 5277072]; GO_function: GO:0016742 -
hydroxymethyl-, formyl- and related transferase activity
[Evidence IEA]; GO_function: GO:0004479 - methionyl-tRNA
formyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0004479 - methionyl-tRNA formyltransferase
activity [Evidence IMP] [PMID 10781559]; GO_function:
GO:0004479 - methionyl-tRNA formyltransferase activity
[Evidence IDA] [PMID 12549912]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0009058 - biosynthetic process [Evidence IEA];
GO_process: GO:0071951 - conversion of methionyl-tRNA to
N-formyl-methionyl-tRNA [Evidence IEA]; GO_process:
GO:0071951 - conversion of methionyl-tRNA to
N-formyl-methionyl-tRNA [Evidence IMP] [PMID 10781559];
GO_process: GO:0071951 - conversion of methionyl-tRNA to
N-formyl-methionyl-tRNA [Evidence IDA] [PMID 12549912];
GO_process: GO:0006412 - translation [Evidence IEA];
GO_process: GO:0006413 - translational initiation
[Evidence IEA,IEA]"
/codon_start=1
/product="Fmt1p"
/protein_id="XP_018736968.1"
/db_xref="GeneID:30033956"
/translation="MLAKRPILGSLWNPCGQKHRLLYIARSYSIAKSPLRIALFGTDN
FTVHSLKALHRYQLANPHVIESLHVITRTPKPSGRGLKVVKEAPSAVYAFEQDIPVLR
AETDSDIVALADHGYNLAIAVSYGKLIPAQFLSQLTYGGINVHPSLLPDLSGAAPLHR
ALLRGYDTTGVTVQTLHPTKFDRGKILLHSPEVHIAKNETLQSLESKLGELGSECLVT
VVDEYRFADPDLNVPTATKYSPSYAPKIQPKEKNINFTKHSLSQILLKHRILGKLAVF
QPTADGVKRIILDNIIDASSLADVVTNEKARLDSLQIGEYDFFKHSDGNSKKAQTVCI
IRVQDGFISPFLLVFQGYSPQTAAQFQKSLRKRAVTSKQLQVS"
gene complement(<336117..>338450)
/gene="VPS13"
/locus_tag="AWJ20_2085"
/db_xref="GeneID:30033957"
mRNA complement(<336117..>338450)
/gene="VPS13"
/locus_tag="AWJ20_2085"
/product="membrane morphogenesis protein VPS13"
/transcript_id="XM_018879013.1"
/db_xref="GeneID:30033957"
CDS complement(336117..338450)
/gene="VPS13"
/locus_tag="AWJ20_2085"
/inference="similar to AA sequence:KEGG_Orthology:K19525"
/codon_start=1
/product="membrane morphogenesis protein VPS13"
/protein_id="XP_018736969.1"
/db_xref="GeneID:30033957"
/translation="MLPNRKAQWSAPFNITNTGRTYVKVFVHGKGTVLLKIDVLLENS
TLFLYFDEAGNNWPYSIRNFSKFEFLFYQANPYVDDQGVEQHPHPPFNPIRYKLPPKS
LMSYAWDFPAAPFKELVILCNGKERRVQLTEIGNLRPMKLPRTERSEGGIIELHVVAD
GPLQSLVLSDYDASSSIYKIKSQSQASSVSQQASSSQVDLFSVDDSVDNDSVLTVTVQ
LEGVGVSLINRRHVELCYMTTRGFEFKYKSSELYETFSMKVKWIQIDNQLYGGHYDVI
LFPSVLPKSTREMENHPTFSAMVTRVKDDSHGVLYIKYASVLLQQMTLEMDEDFLFSL
LDFARDPNTPWTGSEKDILCDESSHIPEPEKESSGEEIYIEVLHIHPAQMDLSFVRTE
RINVEDRSSSPTALNYFVNILTMAIGNINDAPVKLNALLMENVRTQMPVLIQSVESHY
SQGFLYQVHKILGSADFIGNPVGLFNNISSGFVDMFYEPYNGFMLNDRLDEFGIGLAK
GGFSFMKKSIFGISDSVYKVTGSISKGLSVATLDREFQSRRRINMARNRPRHALYGLT
QGATSFFDSVTSGVSGLALAPMEGAAKEGASGFFKGLGKGIVGLPTKTAIGFFDLASN
LSEGVRNTTTVFDGETITRIRPARFIAQDGIVRPYSQREAVGQQWLKSANSGRYFKDE
YLAHINMTQNAAVVIVTTTRIITLFTSNLRTDWEVRFDELRSINRKSTGLTLILRPDD
ERREFFIPIGDQDSQRFLFKEISTAVKEYNRRLQTIS"
gene complement(<339147..>341327)
/gene="VPS13"
/locus_tag="AWJ20_2086"
/db_xref="GeneID:30033958"
mRNA complement(<339147..>341327)
/gene="VPS13"
/locus_tag="AWJ20_2086"
/product="membrane morphogenesis protein VPS13"
/transcript_id="XM_018879014.1"
/db_xref="GeneID:30033958"
CDS complement(339147..341327)
/gene="VPS13"
/locus_tag="AWJ20_2086"
/inference="similar to AA sequence:KEGG_Orthology:K19525"
/codon_start=1
/product="membrane morphogenesis protein VPS13"
/protein_id="XP_018736970.1"
/db_xref="GeneID:30033958"
/translation="MCKVTQYDNRYDILLTIDSPQMILALDYLFALKGFIDFGMSSEG
GEDEVSKVEEIEDTEEESEDTSAASNSDSGMSFSYHINVVDFSVILLADPSLENTEAL
VFKAEQLVLSQRATTTLAVSKVGMFLCDMSHFEEERLRVLDDFSMTASVDTTGSDATH
QLSSVQVSVEPLVLRLSLRDVVLVSNIIQKASALSEGESKITEVEDSDAPTYSRFSKT
EKRKIGGNSKRRPSAVATKSSANGHHRRNSSVSKKLALPRVKVVRGEEMTADFEGLRL
VVIGAAHELPVLDMSVKPFSISARSWSSDLRVDLATESFVNVFNPSKSAWEPLVETWD
IGLNISRSTDVTNTFVNVYSRKPIEVTLTSQTIELLSKSIDFVSANSGNDDLLLRPRD
SDAPYCIRNETGYPIEVWSDSDKDTNEPQVKRIEDGQDIPWRFHDWHELRENLSTDLQ
KLMLGVSLKDSSYDDIRAVSVTSEGEHLHILHRNSQRTGHRLLCDIKLENYHKKITFR
SALNVTNSTHINIHVSTKSDPNQMWTLKPSESRAIPISLVHECNLVVMPDRQLGFDWS
NTAIYWKDLLEAPGSLSCETLPGKPHSFFHFHIEADFNKSLPITWGYPYMTVNICAPV
EIVNLLPYDFDFEIVSKNTTGKRVGWKNSLRKGETSPVHVVKRSDLLLLKVHPATAGY
TESEFAIINVPNHGIYKGKFSREKVMTTHSTDGVQTLNLRIHYL"
gene complement(<341788..>348480)
/gene="VPS13"
/locus_tag="AWJ20_2087"
/db_xref="GeneID:30033959"
mRNA complement(join(<341788..341800,341872..345791,
348364..>348480))
/gene="VPS13"
/locus_tag="AWJ20_2087"
/product="membrane morphogenesis protein VPS13"
/transcript_id="XM_018879015.1"
/db_xref="GeneID:30033959"
CDS complement(join(341788..341800,341872..345791,
348364..348480))
/gene="VPS13"
/locus_tag="AWJ20_2087"
/inference="similar to AA sequence:KEGG_Orthology:K19525"
/codon_start=1
/product="membrane morphogenesis protein VPS13"
/protein_id="XP_018736971.1"
/db_xref="GeneID:30033959"
/translation="MSDGLTAGSEVALPSVLPFEPTDSSLDPLLGSSAALSKLFKLPL
NVLEGHLGELVLQIPWSNLKNQPVKVVIQDVFLLAEPRLDEDYDPEEESRREQELKKE
RLERLELLEKSTPSQGYSQQDVQKNQSFTESLINKTIENLQITIRNIHIRYEDQTSVP
GHPFSLGFTLEELSALSTDDEWNPKFIQSITHVTRKLAKLNSLAIYWNTDSDSIRRTS
DDESGEKMLDNFRTMIISKEKAERNQYILKPVSGIGRITLNKFPSDSQPKSKVQLIFD
ELGFVFDSDQYRDALWTADLFRLYMNSKEFKRFRPTVSVKEDPKAWFKYAAEAVRSHI
HEKNKPWNWDYLTMRLQIRRRYIDLYKLRTKSSPPGTLSNPDEQAEFKQLEFDLSFED
IKFYRSLAKAELRKEQAKVKEEKKRTILGGTSQSDSGSAAPAASWTSWIWGGGAKNAN
QASNGDDEVTMTEEQRKELYDVIEWDEKAAVEKSVTVPQNQITLEVETALESGSFTIR
NDPHGASHDVAAVYFNGFKAQFLQRPTSFLTSISLQEFRVDDKSEGTLFKQIVTVKPL
SHEDDHTHHNVDSHDSTPSTESESAVTSGNSGESSEDAFFWLSFEKNPLDESADSSLH
VKTKSITIFYNAHFLERVARFFKPPKTHLETIGAIINAAGATVEGIRNQTRIGLEYAL
QEHKTANVKLDLQAPLIVIPLDVTSWKSPCVIIDAGHISLLSELAPSELLDEIKKKKS
HQYKERDWERLESLMYDKFKLQFHDTQILLGNNVRDTMKQLHQENSENPAHVIDRINM
DFLVEISILPEAYSLTKFKVTGHLPLFSASMSDAKYKLMMQIIDKSIPNFNFDDDEDI
EDLSDPDPALPPSEIFSRPAAVEHDYGFDSDEDKEPDGETSDAQSVTSTSTADIGSST
VASSIQANATSESQNIFVFVFKIDKVELSLHRCIDDSTLEQSPLVDMVMEQFELEYCF
RSLEMTADVTLGSLRIEDYIDTKCPSELRQIVSSSNSGDSTSDLFKVKYRRIKLPIIK
KTGEEPSDQIVEVSMSTIRFVVEPKTFLTLLDFIITTFTNPEEAEASDSPRIEDVEPA
AGQDSPQESVAPAGQGSIDVRVDLRSIILILNDEGLQLGTLKLDSALVSVRLFGETMN
IHSRIGGFSLQDDTGETQIPSDSNLNQLISIEGDELADFRYETFDRSRKDLSYNSSIY
FRLGSLRVNVIEKPLSRIIGFASRLLQMKGLYDRARQLAMNQANQIESPDKIRFDVII
KTPIFFFPTISADSGDKYDSVTANLGEIYLQNKFESVDGVEDVAINNVSAGVRDMKLT
SRFYFEESWSQNLEIIDKIDILFDISKAD"
gene <347195..>350866
/gene="PIM1"
/locus_tag="AWJ20_2088"
/db_xref="GeneID:30033960"
mRNA <347195..>350866
/gene="PIM1"
/locus_tag="AWJ20_2088"
/product="ATP-dependent Lon protease PIM1"
/transcript_id="XM_018879016.1"
/db_xref="GeneID:30033960"
CDS 347195..350866
/gene="PIM1"
/locus_tag="AWJ20_2088"
/inference="similar to AA sequence:KEGG_Orthology:K08675"
/note="ATP-dependent Lon protease; involved in degradation
of misfolded proteins in mitochondria; required for
biogenesis and maintenance of mitochondria; GO_component:
GO:0005759 - mitochondrial matrix [Evidence IEA,IEA];
GO_component: GO:0005759 - mitochondrial matrix [Evidence
IMP] [PMID 8146662]; GO_component: GO:0005759 -
mitochondrial matrix [Evidence IMP] [PMID 8276800];
GO_component: GO:0042645 - mitochondrial nucleoid
[Evidence IBA]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA,IEA]; GO_function:
GO:0004176 - ATP-dependent peptidase activity [Evidence
IEA,IEA]; GO_function: GO:0004176 - ATP-dependent
peptidase activity [Evidence IMP] [PMID 8146662];
GO_function: GO:0004176 - ATP-dependent peptidase activity
[Evidence IMP] [PMID 8276800]; GO_function: GO:0016887 -
ATPase activity [Evidence IEA]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0070361
- mitochondrial light strand promoter anti-sense binding
[Evidence IBA]; GO_function: GO:0017111 -
nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_function: GO:0008233 - peptidase activity
[Evidence IEA]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IEA]; GO_function:
GO:0004252 - serine-type endopeptidase activity [Evidence
IEA,IEA]; GO_function: GO:0008236 - serine-type peptidase
activity [Evidence IEA]; GO_function: GO:0003697 -
single-stranded DNA binding [Evidence IBA]; GO_function:
GO:0003727 - single-stranded RNA binding [Evidence IBA];
GO_process: GO:0006200 - ATP catabolic process [Evidence
IEA,IEA]; GO_process: GO:0034599 - cellular response to
oxidative stress [Evidence IBA,IEA]; GO_process:
GO:0051131 - chaperone-mediated protein complex assembly
[Evidence IEA]; GO_process: GO:0051131 -
chaperone-mediated protein complex assembly [Evidence IGI]
[PMID 8810243]; GO_process: GO:0006515 - misfolded or
incompletely synthesized protein catabolic process
[Evidence IEA,IEA]; GO_process: GO:0006515 - misfolded or
incompletely synthesized protein catabolic process
[Evidence IMP] [PMID 16336126]; GO_process: GO:0006515 -
misfolded or incompletely synthesized protein catabolic
process [Evidence IGI,IMP] [PMID 7957078]; GO_process:
GO:0007005 - mitochondrion organization [Evidence IBA];
GO_process: GO:0070407 - oxidation-dependent protein
catabolic process [Evidence IBA,IEA]; GO_process:
GO:0030163 - protein catabolic process [Evidence IEA];
GO_process: GO:0051260 - protein homooligomerization
[Evidence IBA]; GO_process: GO:0006508 - proteolysis
[Evidence IEA,IEA]; GO_process: GO:0090296 - regulation of
mitochondrial DNA replication [Evidence IEA]; GO_process:
GO:0001666 - response to hypoxia [Evidence IBA]"
/codon_start=1
/product="ATP-dependent Lon protease PIM1"
/protein_id="XP_018736972.1"
/db_xref="GeneID:30033960"
/translation="MLSSLKTTGRARRGADRLICQIQNARQCRSYHGLPRINKSIVDT
RGVLLSGSHRERDSSRIFATGISQVPRFTTAQFSTSRFVLNNSTGNGDGSNGKNDDLQ
KVNNDQINESKEKELEKEKEQENSVESSSSGTGSEDTAASQTSGKKKSTTSETSKRSR
RSLSPKVSESESATEAAAPTPLRKAVKPSPSAASILFADSNTANSQGSSDSSSGGNNS
NNASENGNAKKQPISPLKQVLPVVLSHRPVFPGLAVHCNSTDETFIAAIDALEKTKSW
DRTVVAFLRKPVEDEEPTVKSSSSGTMTGSVGPSASHSTTTQLMTNVEEAYSVGCVCR
VETIEVTASDGKLIFNSSLFPLYRVRAGELTEPVKDAPVIRQSAAILDADTGLDVTSF
DKAAEEPNNGSSEESVGSNGSTDGSATSEPAVKPSDIVNVHWMKGITAVSDEPYAVDD
RAIQELCFKIIDVLYSISNTSYPLKVQVEKFSKLVRRSPGEDFQKPDFLADFCAALCS
GDKEIQAILETANIKTRLEASLDLLNRELLSIKMHEKISKAIAEQTAQRQREFILQEQ
YKYIKKELGMDDEKGKLAQKFLERADALAMPEEVRKVFDEELAKFRGLESTAGEYSTV
KSYLDWLVSLPWGKFSTDKFDLNAAKKLLDDQHFGLKEVKDRILEFIAVAKLSGSVDG
KILCFAGPPGVGKTSVARSIAEALNRKFDRFSVGGLYDASEIKGHRRTYVAALPGKII
QALKKCETQNPVILIDEIDKVGTSSVHGDPSAALLEVLDPEQNKNFQDIYLDVPVDIS
KILFICTANTLDTLPQPLKDRMEIIHIPGYVNSEKVSIAEGYLTPSLRKKSGLENVDI
KLSSDALLKLVTSYTRESGVRGLNKILEKIYRKVAYNVVIEKEKDTPPIASASESAAE
AAATETASESKDAVADQPKSETVVTADAKEVAPEPIDPELLKDYHLEINVDDLTKYVG
APLYNTVRLYEKFPPGVTMGLAYTPLGGVPLFVETVLQQPLDPKSTPKLTRTGHLGEV
MNESSSIAYSFSRMYMVKRFLGNRFLDHAVLHTHFPEGAVKKDGPSAGIAMATSLISL
ALNKPVNPDVCMTGEITLTGKVLQIGGLREKSAAAKAAGSKIIVFPKDNMAEWEDLPE
MIREGLTPMPVSWYDEVFDIAFGQVDQAEIESMWSKELATKDVDNSTLGRNDDAGFTP
PPPPPPSNRSPSLRASSPY"
gene complement(<350365..>350730)
/locus_tag="AWJ20_2089"
/db_xref="GeneID:30033961"
mRNA complement(<350365..>350730)
/locus_tag="AWJ20_2089"
/product="hypothetical protein"
/transcript_id="XM_018879017.1"
/db_xref="GeneID:30033961"
CDS complement(350365..350730)
/locus_tag="AWJ20_2089"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736973.1"
/db_xref="GeneID:30033961"
/translation="MDSISAWSTWPKAMSNTSSYHETGMGVSPSRIISGRSSHSAMLS
LGKTMILDPAALAAADFSLKPPIWRTLPVRVISPVMQTSGLTGLFKANEIRLVAIAIP
ADGPSFLTAPSGKCVWSTA"
gene <351424..>352278
/gene="YIP5"
/locus_tag="AWJ20_2090"
/db_xref="GeneID:30033963"
mRNA <351424..>352278
/gene="YIP5"
/locus_tag="AWJ20_2090"
/product="Yip5p"
/transcript_id="XM_018879018.1"
/db_xref="GeneID:30033963"
CDS 351424..352278
/gene="YIP5"
/locus_tag="AWJ20_2090"
/note="Protein that interacts with Rab GTPases; localized
to late Golgi vesicles; computational analysis of
large-scale protein-protein interaction data suggests a
possible role in vesicle-mediated transport; GO_component:
GO:0005794 - Golgi apparatus [Evidence IDA] [PMID
17178117]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_function: GO:0017137 - Rab GTPase binding
[Evidence IPI] [PMID 11943201]; GO_process: GO:0008150 -
biological_process [Evidence ND]"
/codon_start=1
/product="Yip5p"
/protein_id="XP_018736974.1"
/db_xref="GeneID:30033963"
/translation="MGSQAYAPPDFESSGSGAYNVSGSMASAGPGNPLLGSGNKTGSI
FSFDFYQWYFNVDTPVVLYRCLLALNPFNNTPFIDSEADLNVGSPAGLIAGGDGNPDL
YGPFWLCTTVIFVLFFASTLTGLLFTAWQGGKYDYKFDLLTGAAGLMYGYTFVIPTGL
WLIVRYLNISPSTTLLQLISLYGYSNVTWIPVAILSISPLLGTPTLSNVIRWIMIALG
FTLSGSFIGKNVYRALIPSQQSDSFVIDRKPALSVLIGVIALHMGLSFAVKILFFGTV
TAPPPPTN"
gene complement(<352506..>354461)
/gene="YVC1"
/locus_tag="AWJ20_2091"
/db_xref="GeneID:30033964"
mRNA complement(<352506..>354461)
/gene="YVC1"
/locus_tag="AWJ20_2091"
/product="Yvc1p"
/transcript_id="XM_018879019.1"
/db_xref="GeneID:30033964"
CDS complement(352506..354461)
/gene="YVC1"
/locus_tag="AWJ20_2091"
/note="Vacuolar cation channel; mediates release of Ca(2+)
from the vacuole in response to hyperosmotic shock;
GO_component: GO:0000324 - fungal-type vacuole [Evidence
IDA] [PMID 11427713]; GO_component: GO:0000324 -
fungal-type vacuole [Evidence IDA] [PMID 11781332];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IDA] [PMID 20035756]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005774 - vacuolar membrane [Evidence
IEA]; GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0005227 - calcium activated cation channel
activity [Evidence IDA] [PMID 1700419]; GO_function:
GO:0005262 - calcium channel activity [Evidence IEA];
GO_function: GO:0005262 - calcium channel activity
[Evidence IDA] [PMID 11781332]; GO_function: GO:0005216 -
ion channel activity [Evidence IEA]; GO_function:
GO:0005267 - potassium channel activity [Evidence IDA]
[PMID 1700419]; GO_function: GO:0005272 - sodium channel
activity [Evidence IDA] [PMID 1700419]; GO_function:
GO:0005244 - voltage-gated ion channel activity [Evidence
IDA] [PMID 1700419]; GO_process: GO:0070588 - calcium ion
transmembrane transport [Evidence IEA]; GO_process:
GO:0006816 - calcium ion transport [Evidence IEA];
GO_process: GO:0030003 - cellular cation homeostasis
[Evidence IDA,IMP] [PMID 11427713]; GO_process: GO:0030003
- cellular cation homeostasis [Evidence IDA,IMP] [PMID
11781332]; GO_process: GO:0006811 - ion transport
[Evidence IEA,IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Yvc1p"
/protein_id="XP_018736975.1"
/db_xref="GeneID:30033964"
/translation="MSTDLASVPAPLVPAQDDLPLVQVSPKQVLKIALRVKKLIDTVV
PIQLTEEEVIKPSSIVLTEKIFQLILEAAGGKGDGAPGTSSRRYRATLVFVLLVIKKW
NRRAALSLLYDSDLYNTRAFVAECYAKRILDTEQDEYYMFRDMLCQRYSITVHGSDSA
PANALELAADLHSTVVIGSSGYQRCMKWLWRGWMVQSDQTPTEYDFYAKVNDPRFWSH
FDPDRIKTPKYQNYIQLLVSLIYLAFYSYAVNNANPSASLIASELWLYLFTFGFILDE
INKLLNVGYAYIGFWNTFNDTLYILVVVAFGFRCAALTYSKSSDQRLSYDLAAYHILS
CAAPLIWGRLLLFLDSVRFFGAMLIVLKELMKESIIFFVLLVVVAGGFLQAFIGLDIA
DGTRDVTMLVVQVMTRTVLDGIDFEWMDGFAPPYGEVLYYIFTFLVSTLLLNILVALF
NSAYQNIYDNATDEYLASMAQKTLRFIRAPDENVFIPPLNLLELAFLILPFEWWMDAS
MYQRINRIFMSIIYSPFLLLIAVDEAKAARRVLYNRSKGVHDDANEEDEEWDLFDGFT
IDAHGIHSYGAEAVQMEMAIEAGDPEFKIDEDAFQKKVEKQVPNFDPQRQTGISAAKA
EELIAKIDALSALVEKLTLEKGQSSSS"
gene <356207..>358228
/gene="CIR2"
/locus_tag="AWJ20_2092"
/db_xref="GeneID:30033965"
mRNA <356207..>358228
/gene="CIR2"
/locus_tag="AWJ20_2092"
/product="Cir2p"
/transcript_id="XM_018879020.1"
/db_xref="GeneID:30033965"
CDS 356207..358228
/gene="CIR2"
/locus_tag="AWJ20_2092"
/inference="similar to AA sequence:KEGG_Orthology:K00311"
/note="Putative ortholog of human ETF-dH; found in a large
supramolecular complex with other mitochondrial
dehydrogenases; may have a role in oxidative stress
response; ETF-dH is also known as electron transfer
flavoprotein dehydrogenase; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence
IDA,IPI] [PMID 11502169]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0051539 - 4 iron, 4
sulfur cluster binding [Evidence IEA]; GO_function:
GO:0004174 - electron-transferring-flavoprotein
dehydrogenase activity [Evidence IEA,IEA]; GO_function:
GO:0051536 - iron-sulfur cluster binding [Evidence
IEA,IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_function: GO:0016491
- oxidoreductase activity [Evidence IEA,IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]"
/codon_start=1
/product="Cir2p"
/protein_id="XP_018736976.1"
/db_xref="GeneID:30033965"
/translation="MLGYKSTGATLQKSASAGAARVLARNSTKSTTKQFSRCFTALKS
NTITGTQTGTIQNTTNGKLKRNLTRSTLINSRLRTSITHNGRRSYSTSPEEGEAAAEE
EFPELSEPREADEVDVCIVGGGPAGLAAAIRFKQLDLEKGSGELRVIVLEKASDMGAH
TVSGAVLEPRALDELLPEWRDDPSLPESVTPVTSEAMRFLTKSMTIPLPEPPQMKNHG
NYIVSLNQMVKWLSEKAEEIGVEVYPGFSVSELVYDEQGRVKGIATNDQGVSRDFKPK
ASFQRGMEFHAKITLLAEGCHGSLSKQVIKKYDLRKDSDPQTYGLGIKEVWEVPKEKF
NKGFVGHSMGWPLSSDVYGGGFIYHFGDGLVTVGAVIGLDYKNPWISPYQEFQRMKHH
PFYANTLKGGKCISYAARALNEGGLQSIPKLSFPGGGLIGCTAGFVNVPKIKGTHTAM
KTGMLAAESAFDSISAFNSAIEEGQSESEVTAPSVESYEESFKDSWVYKELHEIRNVR
PSFNNPLKMWGGVMYSGLDTLILKGRTPWTFHHKHTDSGATEPASKYNKIEYPKPDGE
LSFDILTSVSRTGTNHDEDEPCHLRIPDQDNRKHTEIAYPKYQGIENRFCPAGVYEYI
EDASEPLGVRFQINSQNCIHCKTCDIKVPTQDINWTVPEGGDGPKYYMT"
gene <360167..>361426
/gene="NMA1"
/locus_tag="AWJ20_2093"
/db_xref="GeneID:30033966"
mRNA <360167..>361426
/gene="NMA1"
/locus_tag="AWJ20_2093"
/product="nicotinamide-nucleotide adenylyltransferase
NMA1"
/transcript_id="XM_018879021.1"
/db_xref="GeneID:30033966"
CDS 360167..361426
/gene="NMA1"
/locus_tag="AWJ20_2093"
/inference="similar to AA sequence:KEGG_Orthology:K06210"
/note="Nicotinic acid mononucleotide adenylyltransferase;
catalyzes the transfer of the adenylyl moiety of ATP to
nicotinamide mononucleotide to form NAD; involved in
pathways of NAD biosynthesis, including the de novo,
NAD(+) salvage, and nicotinamide riboside salvage
pathways; homolog of human NMNAT; NMA1 has a paralog,
NMA2, that arose from the whole genome duplication;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0000309
- nicotinamide-nucleotide adenylyltransferase activity
[Evidence IEA]; GO_function: GO:0000309 -
nicotinamide-nucleotide adenylyltransferase activity
[Evidence IDA] [PMID 10428462]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_function: GO:0016779
- nucleotidyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_process: GO:0009435 - NAD biosynthetic process
[Evidence IEA,IEA]; GO_process: GO:0009435 - NAD
biosynthetic process [Evidence IDA] [PMID 10428462];
GO_process: GO:0009058 - biosynthetic process [Evidence
IEA]; GO_process: GO:0019363 - pyridine nucleotide
biosynthetic process [Evidence IEA]"
/codon_start=1
/product="nicotinamide-nucleotide adenylyltransferase
NMA1"
/protein_id="XP_018736977.1"
/db_xref="GeneID:30033966"
/translation="MDPSLAPDFIPPSEQRNRVPTTAGNRVPTVSPIQAYELEEAFDS
QLDINSSHFGTAFPAARRRSSTIEIDMADISQNIPRISSAMSASPTDSDLESNNGREK
FTNTDGGDNASNLKSFDSNQRLASPLAASNYNEPTARQIIQSADLEEVPLGVTRQAKK
LVDYQFPVHRLADTMLDDSKVPLVLVACGSFSPITYLHLRMFEMAMDSIREHTRLEVI
GGYYSPVSDNYQKPGLAKSHHRVRMCELACERTSSWLMVDAWESLQPTYTRTALVLDH
FNEEINVRRQGVGTADGQRKPVKIMLLAGGDLIESMGEPNVWAEDDLHHILGRYGCII
VERTGSDVRSFLLSHDIMYEHRRNVLVIKQLIYNDISSTKVRLFLRRGMSVQYLLPNS
VIRYILEHGLYINESEPVKQVLSEKGD"
gene <367021..>368586
/locus_tag="AWJ20_2094"
/db_xref="GeneID:30033967"
mRNA <367021..>368586
/locus_tag="AWJ20_2094"
/product="hypothetical protein"
/transcript_id="XM_018879022.1"
/db_xref="GeneID:30033967"
CDS 367021..368586
/locus_tag="AWJ20_2094"
/inference="similar to AA sequence:KEGG_Orthology:K08150"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736978.1"
/db_xref="GeneID:30033967"
/translation="MSSVESTSLLRGEEEDVSGSVPVSAVSSVPEILSDVDKEVDGVN
LYMLLCIVCACVGGLMFGYDTGCISQVIELLSDDLGHYLVAWELELITSSTSLFALMG
SIFAGISADRYGRKVLIAVSCVVFILAALVMALSMSLAWLVLGRSLVGLAVGVASTIV
SVYIAEISPPRYRGRLVALNSVSTTGGQVLAYLSGVAFHKTTNGWRYIVGLSGIPPLL
FLLVMTAVPESPRFLFMNGRVDEAKQVLLKLDPRVDVAQVEEMVRQDLSYKDANNGVD
DSSLARLFSVPANFRALVVGCGLMAAQQLCCFNGFMYFSVSLFAMAGFDNPFAVSLVV
SITNFVFTWGAVKYIDKVGRRAILLRSVWIMVVALIVVAFAFTTIDPSAIKNATNNDS
GKPGSDSEPDGMKILTVAATIVFVASYAIGLGNIPWQAIEFLPLDVRSLGSMFITSTN
WTFNTLVSASFLHLLKWLTGTGTFLLYAFFTLLAYIGIYLYYPEVAGLPLEKVNDIFK
QGWRGLGKRGVVL"
gene complement(<368795..>371680)
/gene="PAN2"
/locus_tag="AWJ20_2095"
/db_xref="GeneID:30033968"
mRNA complement(<368795..>371680)
/gene="PAN2"
/locus_tag="AWJ20_2095"
/product="Pan2p"
/transcript_id="XM_018879023.1"
/db_xref="GeneID:30033968"
CDS complement(368795..371680)
/gene="PAN2"
/locus_tag="AWJ20_2095"
/inference="similar to AA sequence:KEGG_Orthology:K12571"
/note="Essential subunit of the Pan2p-Pan3p
poly(A)-ribonuclease complex; complex acts to control
poly(A) tail length and regulate the stoichiometry and
activity of postreplication repair complexes;
GO_component: GO:0031251 - PAN complex [Evidence IDA]
[PMID 8816488]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_function: GO:0004527 -
exonuclease activity [Evidence IEA,IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0004518 - nuclease activity [Evidence
IEA]; GO_function: GO:0003676 - nucleic acid binding
[Evidence IEA]; GO_function: GO:0004535 - poly(A)-specific
ribonuclease activity [Evidence IEA]; GO_function:
GO:0004535 - poly(A)-specific ribonuclease activity
[Evidence IMP] [PMID 8550599]; GO_process: GO:0090503 -
RNA phosphodiester bond hydrolysis, exonucleolytic
[Evidence IEA]; GO_process: GO:0031124 - mRNA 3'-end
processing [Evidence IMP,IPI] [PMID 8550599]; GO_process:
GO:0006397 - mRNA processing [Evidence IEA]; GO_process:
GO:0006397 - mRNA processing [Evidence IMP,IPI] [PMID
8550599]; GO_process: GO:0090305 - nucleic acid
phosphodiester bond hydrolysis [Evidence IEA,IEA];
GO_process: GO:0006301 - postreplication repair [Evidence
IGI,IPI] [PMID 11953437]"
/codon_start=1
/product="Pan2p"
/protein_id="XP_018736979.1"
/db_xref="GeneID:30033968"
/translation="MLSNVTKMKRDSQAIALGLASGSVDVVDPNSLQVVKTFNSHSGP
ISDLDCRQHIIMTCGTSLRKQGYVPDPLVNCYDLRAMKPLPPIPFPAGAAYIKIHPKM
STCCIIASQSGQLQFADSANPTSVFIHQAAITSPLTSLELSPSGDYLSLTDEEGYIHL
WSNTVNPSASFSEFSAPLDFPTSQDPPLHFGVDDDVSLATVGMPYYKEELLSSWSNED
MVFKLGMPSPKIDRSILAGMKVVDYVGYGSYKKTREPRNMAQKYISLDSNRRSSITVP
RFISEKERTGEIDDESAIFDDDVRDDDKRPNGKTNTQAPSSRKITKMYRRLEIKYSKF
GVNDFDFDFYNKTQYAGLENQAANTYCNSILQLYRYSKYMYNFALKKLAVATTVERVS
LLSELGFVFDMLHLAGGKHCCTANFVNALSSIPEAVALGLVFDDMVPNLDQPGLVLQK
FKTFLMEQIVKNEKYQTLSVENQLQNVHSNPAALATLPSEFESSVAGIPVSTSIRCLS
CGTDSIKKSTIYELDLQTEYGFRNNSSAGPGPNTANFDSLFFKALNSSLDKTNKVRGW
CDVCRKYQQLVTTRSATKLPQVLNVNINIFPEADTSVSRQIWASPHWPPTEFFTDNHS
RRLVANHRVGKDKYELLGCVVEISRPGHSDHHLVSVVKIDEAWYLFNDFLVKQIPEKD
ALDFSLAWKTPVLLVYQLANEMPKEFDYGSWKQTLDTSILYKDFFTAGNREEVRREYE
LLTLEEAPKPGTLVAIDAEFVMLQNEETEIRSDGTKSIVKPRSLSLARVSVVRGEGPK
EGVAFIDDYIATTDTIVDYLTEFSGIEPGDLDPALSKRGLVSLHTAYKRLWLLLQLGV
TFVGHGLSNDFRTINIQVPANQVIDTLDIYFVRARQRKLSLKFLAWYLLDENVQTGNH
DSIEDARTALHLYKKHQQLLRDGTFDATLAKVYSEGHKYNFRPPANS"
gene complement(<374204..>374277)
/locus_tag="AWJ20_2096"
/db_xref="GeneID:30033969"
tRNA complement(<374204..>374277)
/locus_tag="AWJ20_2096"
/product="tRNA-Ile"
/db_xref="GeneID:30033969"
gene complement(<377609..>379693)
/gene="GSH1"
/locus_tag="AWJ20_2097"
/db_xref="GeneID:30033970"
mRNA complement(<377609..>379693)
/gene="GSH1"
/locus_tag="AWJ20_2097"
/product="glutamate--cysteine ligase"
/transcript_id="XM_018879024.1"
/db_xref="GeneID:30033970"
CDS complement(377609..379693)
/gene="GSH1"
/locus_tag="AWJ20_2097"
/inference="similar to AA sequence:KEGG_Orthology:K11204"
/note="Gamma glutamylcysteine synthetase; catalyzes the
first step in glutathione (GSH) biosynthesis; expression
induced by oxidants, cadmium, and mercury; protein
abundance increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IBA];
GO_component: GO:0005622 - intracellular [Evidence IDA]
[PMID 2184310]; GO_function: GO:0005524 - ATP binding
[Evidence IEA]; GO_function: GO:0004357 -
glutamate-cysteine ligase activity [Evidence IEA,IEA];
GO_function: GO:0004357 - glutamate-cysteine ligase
activity [Evidence IDA] [PMID 1687097]; GO_function:
GO:0004357 - glutamate-cysteine ligase activity [Evidence
IMP] [PMID 2184310]; GO_function: GO:0016874 - ligase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006750
- glutathione biosynthetic process [Evidence IEA,IEA,IEA];
GO_process: GO:0006750 - glutathione biosynthetic process
[Evidence IDA] [PMID 1687097]; GO_process: GO:0006750 -
glutathione biosynthetic process [Evidence IMP] [PMID
8768524]; GO_process: GO:0046686 - response to cadmium ion
[Evidence IDA] [PMID 10921921]; GO_process: GO:0042542 -
response to hydrogen peroxide [Evidence IMP] [PMID
7615092]"
/codon_start=1
/product="glutamate--cysteine ligase"
/protein_id="XP_018736980.1"
/db_xref="GeneID:30033970"
/translation="MGLLSLGTPLEWNEVSQYTDHVREHGIEQLIHIHNANKDKKNDS
LLWGDEVEYMVVELDPESKEAKLSLRQAQILDDLAQAEKKGDLTAHNVVFHPEYGRYM
LEATPAKPFTGDFKDLLLVEANMATRRTIAKEHMNSNEFPLTLTSYPRLGVGQFTSPA
AEPNGKASRSFFLPDEIINPHVRFPTLTANIRTRRGSKVAINVPIYKDEKTPSPFVDD
SIPWDRDLYPEDANAREGAALPDHIYMDSMGFGMGCSCLQITFQATDIDEGRDLYDQL
APLAPILLALTAAAPAYRGYLSDQDARWNVIAGSVDDRTPVERGLVPADDINEESEDS
EVAQKAENNENSEKNEKTNGKAQFCSSQSNRTKTTSTTKYPSVKANSDRVIPKSRYDS
IDSYISGHLKGSASHFNDTKLVINKKVKKRLTEAGFDEQLSNHFAHLFIRDPIVIFKE
LLDIDDTKESDHFENIQSTNWQTMRFKPPPPSEGPNQNVGWRVEFRPMEVQITDFENA
AFSVFIVLVTRVILSYKLNFYIPISKVDENMKVAHYRDAVNKNKFWFRTDIESPTSQP
DTETEPSPGIYELLSINEIINGKADKSFIGLVPLIRRYLNYINIDLDTLCQLDRYLSL
ISRRASGELWTAATWMRHFITSHPKYNKDSKISSDINYDLMQKVYDITQGKGWDDGLT
KHFLGGFSSSVP"
gene <390180..>391613
/gene="CUP9"
/locus_tag="AWJ20_2098"
/db_xref="GeneID:30033971"
mRNA <390180..>391613
/gene="CUP9"
/locus_tag="AWJ20_2098"
/product="Cup9p"
/transcript_id="XM_018879025.1"
/db_xref="GeneID:30033971"
CDS 390180..391613
/gene="CUP9"
/locus_tag="AWJ20_2098"
/note="Homeodomain-containing transcriptional repressor;
regulates expression of PTR2, which encodes a major
peptide transporter; imported peptides activate
ubiquitin-dependent proteolysis, resulting in degradation
of Cup9p and de-repression of PTR2 transcription; CUP9 has
a paralog, TOS8, that arose from the whole genome
duplication; protein abundance increases in response to
DNA replication stress; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IC] [PMID 9427760]; GO_function: GO:0003677 -
DNA binding [Evidence IEA,IEA]; GO_function: GO:0000978 -
RNA polymerase II core promoter proximal region
sequence-specific DNA binding [Evidence IDA] [PMID
9427760]; GO_function: GO:0001103 - RNA polymerase II
repressing transcription factor binding [Evidence IPI]
[PMID 18708352]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IEA]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 19111667]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IDA] [PMID
19158363]; GO_function: GO:0003700 - sequence-specific DNA
binding transcription factor activity [Evidence IEA];
GO_function: GO:0001133 - sequence-specific transcription
regulatory region DNA binding RNA polymerase II
transcription factor recruiting transcription factor
activity [Evidence IC] [PMID 18708352]; GO_function:
GO:0001133 - sequence-specific transcription regulatory
region DNA binding RNA polymerase II transcription factor
recruiting transcription factor activity [Evidence IC]
[PMID 9427760]; GO_process: GO:0035955 - negative
regulation of dipeptide transport by negative regulation
of transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 10850718]; GO_process: GO:0035955 - negative
regulation of dipeptide transport by negative regulation
of transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 17005992]; GO_process: GO:0035955 - negative
regulation of dipeptide transport by negative regulation
of transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 18708352]; GO_process: GO:0035955 - negative
regulation of dipeptide transport by negative regulation
of transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 9427760]; GO_process: GO:0035952 - negative
regulation of oligopeptide transport by negative
regulation of transcription from RNA polymerase II
promoter [Evidence IMP] [PMID 17005992]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]"
/codon_start=1
/product="Cup9p"
/protein_id="XP_018736981.1"
/db_xref="GeneID:30033971"
/translation="MNTSDQTNRSSLPRSSNSDSPLSVSNMSMSIAASSVQPQWQMSA
NDTPVLPHERSNIRLPSINELMASFSNEPQQSVPRANSSSYYSPAVNVKPEPHSPPFY
TQSSQQQQQHPPAHSHIPLQSHLSQPEPRSEHASLPHPSHPYQPSQQPQQQQPPQLQQ
PHPHQQQQHGQQLPHGQQQLQPLQQSHTQYQQGPVSPSLASSSSSNMDSAPAYSTTSS
GQPGSDGHSNTSIQMLLDASSSADSSQKKRSHSVSSASSVSSNSNRMPPTSGGAMNNN
STPGNTSNGSASSSSPPFSISPPPFSASPYSPPLKRRGNLPRAVTDILRNWLSSHLHY
PYPTEQEKIELMKQTGLSLNQVSNWFINARRRRVPAIYKHSGLMNMTERPRRTVSEFN
MRHSPQQQPMMNHQRSVSQIHHPQQPPQPQPQQQPPPPPMSQPTPPPPPPSMTNPLQQ
QAPTPAAPPPNHMHYSSQPHIPPQWKP"
gene complement(<392070..>394457)
/gene="CTI6"
/locus_tag="AWJ20_2099"
/db_xref="GeneID:30033972"
mRNA complement(<392070..>394457)
/gene="CTI6"
/locus_tag="AWJ20_2099"
/product="Cti6p"
/transcript_id="XM_018879026.1"
/db_xref="GeneID:30033972"
CDS complement(392070..394457)
/gene="CTI6"
/locus_tag="AWJ20_2099"
/note="Component of the Rpd3L histone deacetylase complex;
relieves transcriptional repression by binding to the
Cyc8p-Tup1p corepressor and recruiting the SAGA complex to
the repressed promoter; contains a PHD finger domain;
GO_component: GO:0033698 - Rpd3L complex [Evidence IDA]
[PMID 16286007]; GO_component: GO:0033698 - Rpd3L complex
[Evidence IDA] [PMID 16286008]; GO_component: GO:0033698 -
Rpd3L complex [Evidence IDA] [PMID 16314178];
GO_component: GO:0033698 - Rpd3L complex [Evidence IDA]
[PMID 19040720]; GO_component: GO:0070210 - Rpd3L-Expanded
complex [Evidence IDA] [PMID 19040720]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IC] [PMID 12086626];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0035064 - methylated histone binding
[Evidence IDA] [PMID 17142463]; GO_function: GO:0008134 -
transcription factor binding [Evidence IDA] [PMID
12086626]; GO_function: GO:0008270 - zinc ion binding
[Evidence IEA]; GO_process: GO:0016568 - chromatin
modification [Evidence IEA]; GO_process: GO:0061188 -
negative regulation of chromatin silencing at rDNA
[Evidence IMP] [PMID 16286008]; GO_process: GO:0061186 -
negative regulation of chromatin silencing at silent
mating-type cassette [Evidence IMP] [PMID 16286008];
GO_process: GO:0031939 - negative regulation of chromatin
silencing at telomere [Evidence IMP] [PMID 16286008];
GO_process: GO:0045893 - positive regulation of
transcription, DNA-templated [Evidence IMP] [PMID
12086626]; GO_process: GO:0030174 - regulation of
DNA-dependent DNA replication initiation [Evidence IMP]
[PMID 19417103]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Cti6p"
/protein_id="XP_018736982.1"
/db_xref="GeneID:30033972"
/translation="MVGKRAGRSRSAQAGEVSDSTSSTPNGDSGNSSRRSRRHASSLL
EGDDEMTEENDTSRPHDEDSADNSSSINNNSSSKEKGISSTPTRRRTRKGTEGVADSV
LVAEEATLSSTPVKSQVKNEDGDDTIGEDEMKLEYDNDNEKEDEIVHDADVTIEEEGN
TTKELMSDEEAEPEADLDPEPEAELKNEPTGKEEQEEEEDDVEEEHIGRRGKREKRER
KTKVEPEDDTGAAGDDESHEDTKDSIDPAPRRRGRGRGKRRRKSPNEEDVELDADGDT
EMVPAEPELEPDLEDADEAQADGASDDIPEEGEEDAEGEDSSVTRCICGHQELQTHRI
SKTKHSSIDSGLFIQCDQCQVWQHGFCVGFSSETEVPDVYYCEWCRPDFHLVVVRPWG
KTSSYLPHADPNANAAKEDDGSSVSNGNDDEHSSGSRSSRAQRHRKDLNERDGYFDKK
EKDEDTSISSIDKTASNGNVAKSVSSPSTRSRRSAAAQGSPTVIRTAHEEAAHRRKTL
NSSRDMSYEETLKRVLEESTYDIQPPNASAANDSDSNTTTTTTSTTRRRRNNNSSQAL
LAAEAAERDRDISSEDQSGSMTSSKRRTRDNTMKPPSRKRAAGGAVNGAGSGAAETGE
GSRASSPVSESRTTGSTGKSKRQRTKGSRNQASSEGNSAGASGASTPKKSATRPVERS
KPRIPPPRSTMSDMRKRVGAILEFIARTQIDHANEQNERHQLLRDRNSLHQQLTGEPP
QTDASSTSDQQPDPTSKFAGLFQLHQSNLDQMDQLTRKLLHWEQLFGRYGERSTH"
gene complement(<394926..>395036)
/locus_tag="AWJ20_2100"
/db_xref="GeneID:30033975"
tRNA complement(<394926..>395036)
/locus_tag="AWJ20_2100"
/product="tRNA-Leu"
/db_xref="GeneID:30033975"
gene complement(<395314..>397683)
/gene="TCO89"
/locus_tag="AWJ20_2101"
/db_xref="GeneID:30033976"
mRNA complement(<395314..>397683)
/gene="TCO89"
/locus_tag="AWJ20_2101"
/product="Tco89p"
/transcript_id="XM_018879028.1"
/db_xref="GeneID:30033976"
CDS complement(395314..397683)
/gene="TCO89"
/locus_tag="AWJ20_2101"
/note="Subunit of TORC1 (Tor1p or
Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1
complex regulates growth in response to nutrient
availability; cooperates with Ssd1p in the maintenance of
cellular integrity; deletion strains are hypersensitive to
rapamycin; GO_component: GO:0031931 - TORC1 complex
[Evidence IPI] [PMID 14736892]; GO_component: GO:0031234 -
extrinsic component of cytoplasmic side of plasma membrane
[Evidence IDA] [PMID 14736892]; GO_component: GO:0000306 -
extrinsic component of vacuolar membrane [Evidence IDA]
[PMID 14736892]; GO_component: GO:0000329 - fungal-type
vacuole membrane [Evidence IDA] [PMID 14562095];
GO_component: GO:0000329 - fungal-type vacuole membrane
[Evidence IDA] [PMID 17560372]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005886 - plasma
membrane [Evidence IEA,IEA]; GO_component: GO:0005774 -
vacuolar membrane [Evidence IEA]; GO_component: GO:0005773
- vacuole [Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0031929 -
TOR signaling [Evidence IC] [PMID 14736892]; GO_process:
GO:0031505 - fungal-type cell wall organization [Evidence
IMP] [PMID 14736892]; GO_process: GO:0006071 - glycerol
metabolic process [Evidence IGI] [PMID 10931309];
GO_process: GO:0001558 - regulation of cell growth
[Evidence IPI] [PMID 14736892]; GO_process: GO:0009651 -
response to salt stress [Evidence IMP] [PMID 17298473]"
/codon_start=1
/product="Tco89p"
/protein_id="XP_018736983.1"
/db_xref="GeneID:30033976"
/translation="MPHTTGPNRVLRQTSGNDSGGSDDSSGSSSNTRKPERPHLTSHN
SSTGGQKLFVARSGHGHAKHFRSSASATRLNKMTRMAPLHGLNDSSVTGRSPNQTSGV
AQGTSGATGAAGAGAGAGSGGGPSSGGSGSRGNAKFLRSKSTDNIYNHNPAEVIHRRN
TSQAMLRSSRSQTRIGRHHPHMHALMPTRSRDSSVSVDQTGGSAATGAGSGSGSGGAV
KRKSTAGMVRAARPVIQLDHDDSDDENDMEESGDESHDKSDARNLDSASKNANANANQ
VPLPVEEQAAQQTRGAVQIQELPPSDSPVSTGSNTNTTNGGADANTNANSNSSRASPL
SPAAGSEAVREPLPTADSTVSKGATTPAISAVTSTSSRQSSPRPGASASVTATATAAF
QSATGRDMTSNNLQPPSLGGQPKSDEEYEDTDADDDDEPPATSQPKHISPTDSFKPLL
HSTNTPTPPQISTETVVAISGSEASTSGSSTTATPYLSRTTSNHLNSDSTGSDPEVVR
SRFLDTSYRSGESTPYPVASWVDLQRISSTTNNTSSSSAVVDPVNGKITNGPADSGIA
SGSGAGNSTGANRVASETALDNPSLVTTRTQQKLWLQRQSIDHPPTPTSTMSLTSEQR
RDFERITKEFINVRRYQSPIAEAVDRIRNRRQPQVSSVALQNRKKRPDTNNSSDPYSN
LSQSLPKRVTSLSATTLNESNGFPTDNTNASNTWKSVTTTTAASAANVGSVAYKLTKI
WNEGWQDPDEPEPPAPQESPSIHSQAQQVQQQIAAAQRQIRSNAAIKLV"
gene <399353..>400633
/gene="VPS45"
/locus_tag="AWJ20_2102"
/db_xref="GeneID:30033977"
mRNA <399353..>400633
/gene="VPS45"
/locus_tag="AWJ20_2102"
/product="Vps45p"
/transcript_id="XM_018879029.1"
/db_xref="GeneID:30033977"
CDS 399353..400633
/gene="VPS45"
/locus_tag="AWJ20_2102"
/note="Protein of the Sec1p/Munc-18 family; essential for
vacuolar protein sorting; required for the function of
Pep12p and the early endosome/late Golgi SNARE Tlg2p;
essential for fusion of Golgi-derived vesicles with the
prevacuolar compartment; GO_component: GO:0000139 - Golgi
membrane [Evidence IDA] [PMID 7720726]; GO_component:
GO:0031201 - SNARE complex [Evidence IPI] [PMID 10397773];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
9624182]; GO_component: GO:0019898 - extrinsic component
of membrane [Evidence IDA] [PMID 7720726]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0005774 - vacuolar membrane [Evidence IEA];
GO_component: GO:0005773 - vacuole [Evidence IEA];
GO_function: GO:0000149 - SNARE binding [Evidence IPI]
[PMID 16769821]; GO_function: GO:0051082 - unfolded
protein binding [Evidence IMP,IPI] [PMID 11432826];
GO_process: GO:0032258 - CVT pathway [Evidence IMP] [PMID
10545112]; GO_process: GO:0006895 - Golgi to endosome
transport [Evidence IGI] [PMID 9335586]; GO_process:
GO:0006896 - Golgi to vacuole transport [Evidence IMP]
[PMID 7720726]; GO_process: GO:0048210 - Golgi vesicle
fusion to target membrane [Evidence IMP] [PMID 9650782];
GO_process: GO:0035543 - positive regulation of SNARE
complex assembly [Evidence IMP] [PMID 11432826];
GO_process: GO:0006623 - protein targeting to vacuole
[Evidence IMP] [PMID 7628704]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]; GO_process: GO:0007035 -
vacuolar acidification [Evidence IMP] [PMID 7628704];
GO_process: GO:0000011 - vacuole inheritance [Evidence
IMP] [PMID 1493335]; GO_process: GO:0007033 - vacuole
organization [Evidence IMP] [PMID 9650782]; GO_process:
GO:0006904 - vesicle docking involved in exocytosis
[Evidence IEA]; GO_process: GO:0016192 - vesicle-mediated
transport [Evidence IEA]"
/codon_start=1
/product="Vps45p"
/protein_id="XP_018736984.1"
/db_xref="GeneID:30033977"
/translation="MKQRPLIRFDRNSSMAKKLAHEVSYIVKQERTQLFDFRKPDTAP
ILLILDRRNDPITPLLTPWTYQAMVHELVGIQNNRVDLSNVPDISKDLKEIVLSADKD
PFFAKTMYLNFGDLGASIKDYVSQYQAKTKSNSVIESIADMKRFVEDYPEFRRLSGNV
SKHVTLVSELSRLVSTNGDLLEVSELEQSLACNDSHNSDMKNVQQMLVQSQVSANSKL
RLVALYALRYQYHSNNATEALLGLLGTQHPEIASKIHAVRILVNSYAGGKQRQENLFA
SSSFFARAQSGFKGLKGVENVYTQHEPLLKTTLTNLIKGKLKKTTHPYYDPGDGSLPS
WAVHGMGSGPGSNSDDGEKPQDIIIFMVGGTTYEEARVVAEMNAVARGVRILIGGTTI
HNSQSFLNDIEDAGSRWPPPPPATASERLASRVH"
gene complement(<401313..>401825)
/gene="CBC2"
/locus_tag="AWJ20_2103"
/db_xref="GeneID:30033978"
mRNA complement(<401313..>401825)
/gene="CBC2"
/locus_tag="AWJ20_2103"
/product="Cbc2p"
/transcript_id="XM_018879030.1"
/db_xref="GeneID:30033978"
CDS complement(401313..401825)
/gene="CBC2"
/locus_tag="AWJ20_2103"
/inference="similar to AA sequence:KEGG_Orthology:K12883"
/note="Small subunit of the heterodimeric cap binding
complex with Sto1p; interacts with Npl3p, possibly to
package mRNA for export from the nucleus; may have a role
in telomere maintenance; contains an RNA-binding motif;
GO_component: GO:0000243 - commitment complex [Evidence
IDA] [PMID 8682299]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005846 - nuclear cap
binding complex [Evidence IEA]; GO_component: GO:0005846 -
nuclear cap binding complex [Evidence IDA] [PMID 8811086];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0048471 - perinuclear region of cytoplasm
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IEA]; GO_function: GO:0000339 - RNA cap binding
[Evidence IEA]; GO_function: GO:0000339 - RNA cap binding
[Evidence IDA,ISS] [PMID 8682299]; GO_function: GO:0003676
- nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0008380 - RNA splicing [Evidence IEA];
GO_process: GO:0045292 - mRNA cis splicing, via
spliceosome [Evidence IEA]; GO_process: GO:0006397 - mRNA
processing [Evidence IEA]; GO_process: GO:0000398 - mRNA
splicing, via spliceosome [Evidence IMP] [PMID 8682299];
GO_process: GO:0051028 - mRNA transport [Evidence IEA];
GO_process: GO:0000184 - nuclear-transcribed mRNA
catabolic process, nonsense-mediated decay [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Cbc2p"
/protein_id="XP_018736985.1"
/db_xref="GeneID:30033978"
/translation="MSRIDNPDLFTVHSTERTDGPSGYILRQAKRSQKGQDDLKAVET
TKTLYVGNLSFYTSEEQIFELFSKAGEITRIIMGLDRFNKTPCGFCFVEYETHKQAVD
CVKYINGTKLDDRVVKVDLDPGFVEGRQYGRGSSGGQVRDEFRDDYDPGRGGYGKRWS
QAAQADRDAL"
gene <404526..>406079
/gene="DJP1"
/locus_tag="AWJ20_2104"
/db_xref="GeneID:30033979"
mRNA <404526..>406079
/gene="DJP1"
/locus_tag="AWJ20_2104"
/product="Djp1p"
/transcript_id="XM_018879031.1"
/db_xref="GeneID:30033979"
CDS 404526..406079
/gene="DJP1"
/locus_tag="AWJ20_2104"
/note="Cytosolic J-domain-containing protein; required for
peroxisomal protein import and involved in peroxisome
assembly, homologous to E. coli DnaJ; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005829 - cytosol [Evidence IDA] [PMID 9679141];
GO_function: GO:0051087 - chaperone binding [Evidence ISS]
[PMID 9679141]; GO_process: GO:0016558 - protein import
into peroxisome matrix [Evidence IMP] [PMID 9679141]"
/codon_start=1
/product="Djp1p"
/protein_id="XP_018736986.1"
/db_xref="GeneID:30033979"
/translation="MVVDTAYYEILEVSTDASELDIKRAYRKMAIRHHPDKNPDDPTA
HERFQAIGEAYQVLSDPDLRKRYDQFGKEQAVPDAGFEDPAEFFTTIFGGEAFADLIG
EISLIKDLTKTMEIASLDDDEDDLVTGEKKSEVKADAEKTSTPGAGGSGLSPTAAAEA
SPLNSASTKTGGHSPSGSATTATSPFPNVQSPQPSYGAAEARPAATSPLPGTKSGKGT
PVLAAITAGPDSDAHSTASSSTATEKKKEKKDKKSKNSLSKEQKAELEQYEIERQKAK
AERLETLRVKLIDRISVWTETGKDDAVTYAFKEKTRLEAENLKMESFGIELLHAIGHT
YYSKGSTLLKSQKFLGISGFFSRVKEKGTLAKDTWNTISSALDAQSSLQEMARAEEKG
GDEWTEERRVEMERTLLGKILAAAWNGSRYEIQSVLREVCDQVLYDKSVPMSKRLERA
QALVIIGSIFKSTVRTAEEAAEAQMFEELVAEAAASKKKKKDKHQHQTAAEKSADAAS
AAASAAASH"
gene complement(<406313..>407005)
/locus_tag="AWJ20_2105"
/db_xref="GeneID:30033980"
mRNA complement(<406313..>407005)
/locus_tag="AWJ20_2105"
/product="hypothetical protein"
/transcript_id="XM_018879032.1"
/db_xref="GeneID:30033980"
CDS complement(406313..407005)
/locus_tag="AWJ20_2105"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736987.1"
/db_xref="GeneID:30033980"
/translation="MSRIETAALETYSACDVADALVKNGLADGGFIPHLVQQSPERPP
ETAVTGPAYTVLYAPIDDPRPAVASHYIDSAPEGSVVVLATTPDTQLSTAPYTTINNA
LYGGLMSTRAKFLGCKGSVVLGKIRDISEHRSLNYPVYSYGLGIAAPNKVAKVVGVNV
PLTVPSSDLTIHPGDQIVGDENGVAVIPQRLLQNVIQDIPPRVKADQLAAEDIKSGVP
AAEAQKTRRKNL"
gene complement(<407804..>409573)
/locus_tag="AWJ20_2106"
/db_xref="GeneID:30033981"
mRNA complement(<407804..>409573)
/locus_tag="AWJ20_2106"
/product="Uric acid-xanthine permease (UAPA transporter)"
/transcript_id="XM_018879033.1"
/db_xref="GeneID:30033981"
CDS complement(407804..409573)
/locus_tag="AWJ20_2106"
/codon_start=1
/product="Uric acid-xanthine permease (UAPA transporter)"
/protein_id="XP_018736988.1"
/db_xref="GeneID:30033981"
/translation="MTASNNIIDPVVVYADEEELPHPIAQTKTATTIRSTAETIRRKI
TTREGILGDFDYAYLFTPRIPFYTSMKERQSSEPTRSSTVFFGLNSSIPLLLGIVLGL
QHCLAMLAGIITPPIIISSMANLTTEDSEYLVSASLIACGFLSMVQIIRFRILKTKYY
IGTGLLSVVGTSFSTISVVSTAFPQFYKNGFCPSDANGKPLPCPDAYGALLGTCAVCG
LIEVLLSFCPPKILQKIFPPLVTGPVVLLIGTSLVQSGFQDWVGGSGCATPGTICPSP
GAPHALPWGSAQFIGLGFLAFVCIVLCEKWGSPFMKSCSIVISLIIGCIVAAACGYFD
RSTIDAAPVANFMWVKTFRLSVAGNMVLPVLSVYIVCMMETIGDVTATSDVSRQPVEG
DLYDSRIQGGVLADGLNSIIAALMTITPMSTFAQNNGVISLTQCANRVAGLWACFFLI
IMGIFSKFAGAIVAIPKPVLGGVTTFLFSSVAVSGIKIISSIPFTRRDRFVLTASLMF
GFAALLVPNWFSQVFTYQGDNNRLKGLLDAVIIFMETPYSIAGFVGVIVNLVIRQEFD
VATSIEDLDGTENVHLPGAETEK"
gene <414602..>416371
/gene="CLG1"
/locus_tag="AWJ20_2107"
/db_xref="GeneID:30033982"
mRNA <414602..>416371
/gene="CLG1"
/locus_tag="AWJ20_2107"
/product="Clg1p"
/transcript_id="XM_018879034.1"
/db_xref="GeneID:30033982"
CDS 414602..416371
/gene="CLG1"
/locus_tag="AWJ20_2107"
/note="Cyclin-like protein that interacts with Pho85p; has
sequence similarity to G1 cyclins PCL1 and PCL2;
GO_component: GO:0000307 - cyclin-dependent protein kinase
holoenzyme complex [Evidence IPI] [PMID 9032248];
GO_function: GO:0016538 - cyclin-dependent protein
serine/threonine kinase regulator activity [Evidence ISA]
[PMID 8212900]; GO_function: GO:0016538 - cyclin-dependent
protein serine/threonine kinase regulator activity
[Evidence IPI] [PMID 9032248]; GO_function: GO:0019901 -
protein kinase binding [Evidence IEA]; GO_process:
GO:0016239 - positive regulation of macroautophagy
[Evidence IMP] [PMID 20417603]; GO_process: GO:0000079 -
regulation of cyclin-dependent protein serine/threonine
kinase activity [Evidence IEA]"
/codon_start=1
/product="Clg1p"
/protein_id="XP_018736989.1"
/db_xref="GeneID:30033982"
/translation="MPGLPYYNDQMVTPPDYLVNHPAYGHNMPATRYASNPAPPQPYS
YGMVAPAQAISAPYDYPYKQSIRYQQLQQQARAHAQAQAQAQAQAQAQQHSQDYQDYQ
QQRQAQSSSVQAHLPHQLQSQPQLQLQMPQTHVYAHQPQSQPGPHQSHTRHHHATSFS
SNGTTFFVPTVPLDQMAPHMAPAGSSNGLRPALGPAIAPVVGQTGVSAPGTGHSVNGT
APAGAAAAAQAQAQPPTGGVSATLDYEIDQMSDFLSTMAFGIMVPMASTNQFTWDNPS
FTAYKKFVCQVLTATRLPKSTIILALVYLSKKWAISKPTVVECDSSVVYTVLVVALLL
ANKFHDDNTFTNKSWNEATGIPVKELTTVEAQWLREIQWSLSLNQNDLKGWQKWNHCW
KVYMSSKAAAVAAATVKQMSPYRSEAKEKYSDGLPSPTSPVYYSRDSSPRSNSPRRAT
YTIPKWYETANNTPVNAISSGRRSASQQHSSAALAAVTSNTSEPFVSYFQQSFAIPGT
SAYDEEKAVDSASYNRRAIPGAYYNYPTHHHHAHHHQYPQSHYMAAYNHLAGCSCSLC
VFEPIKQPAIHNGQWFGSSVVAC"
gene complement(<416740..>418065)
/locus_tag="AWJ20_2108"
/db_xref="GeneID:30033983"
mRNA complement(<416740..>418065)
/locus_tag="AWJ20_2108"
/product="2-aminoadipate transaminase"
/transcript_id="XM_018879035.1"
/db_xref="GeneID:30033983"
CDS complement(416740..418065)
/locus_tag="AWJ20_2108"
/note="Protein with 2-aminoadipate transaminase activity;
shares amino acid similarity with the aminotransferases
Aro8p and Aro9p; YER152C is not an essential gene;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_function: GO:0047536 -
2-aminoadipate transaminase activity [Evidence IDA] [PMID
19342587]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0030170 - pyridoxal
phosphate binding [Evidence IEA]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0009058 -
biosynthetic process [Evidence IEA]"
/codon_start=1
/product="2-aminoadipate transaminase"
/protein_id="XP_018736990.1"
/db_xref="GeneID:30033983"
/translation="MSVSKVKKVNFFRGHPSNSLLGPSSHYVLKASENVLSPAHRPGD
DDDEDRHPLTYGSDPGSLTVRKQIAKWTYPDLSADKEQIYADSINLTNGASFGAGGAL
LQCTSAHSGFTKQAFIVSPTYFLINSVFLDAGFADKFSPIEQRADGSIDLVALEKLLV
HFDKEPEVSYEDAAKYIQDDSRPRRKLYKYVMYLVPTFSNPRGTVIPLSDRLKLLELA
RAHDMLLLCDDVYDWLYYKADSINDLPPRLVTLDRESTTDSSSYGNTISNCTFSKLVG
PGLRVGWQETVSPRLAQQLCQGGAVRSGGTPAHLNTMLVGSLLSLGYMESILKDLRSV
FGDRSKHMRQDFEKYLPQGTQIEGGEGGYFFWVTLPEEYNGRAIAEQCLKKNVVLAEA
HNFQVAHGPTPRVPINDNSFRICFAYHTVEESQQGIQVWGEVCEENKNK"
gene complement(<418614..>421067)
/gene="KIP3"
/locus_tag="AWJ20_2109"
/db_xref="GeneID:30033984"
mRNA complement(<418614..>421067)
/gene="KIP3"
/locus_tag="AWJ20_2109"
/product="tubulin-dependent ATPase KIP3"
/transcript_id="XM_018879036.1"
/db_xref="GeneID:30033984"
CDS complement(418614..421067)
/gene="KIP3"
/locus_tag="AWJ20_2109"
/inference="similar to AA sequence:KEGG_Orthology:K10401"
/note="Kinesin-related antiparallel sliding motor protein
involved in mitotic spindle positioning; sliding activity
promotes bipolar spindle assembly and maintenance of
genome stability; also inhibits spindle elongation and
promotes spindle disassembly in late anaphase;
GO_component: GO:0005737 - cytoplasm [Evidence IEA];
GO_component: GO:0005881 - cytoplasmic microtubule
[Evidence IDA] [PMID 9693366]; GO_component: GO:0005856 -
cytoskeleton [Evidence IEA,IEA]; GO_component: GO:0005871
- kinesin complex [Evidence IEA]; GO_component: GO:0005871
- kinesin complex [Evidence TAS] [PMID 9153752];
GO_component: GO:0005874 - microtubule [Evidence IEA];
GO_component: GO:0005880 - nuclear microtubule [Evidence
IDA] [PMID 9693366]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0008017 - microtubule
binding [Evidence IEA]; GO_function: GO:0003777 -
microtubule motor activity [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_function: GO:0008574 - plus-end-directed microtubule
motor activity [Evidence IDA] [PMID 16906145];
GO_function: GO:0008574 - plus-end-directed microtubule
motor activity [Evidence IDA] [PMID 16906148];
GO_function: GO:0070463 - tubulin-dependent ATPase
activity [Evidence IDA] [PMID 16906148]; GO_process:
GO:0000132 - establishment of mitotic spindle orientation
[Evidence IMP] [PMID 16906148]; GO_process: GO:0000132 -
establishment of mitotic spindle orientation [Evidence
IGI,IMP] [PMID 9281582]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0007018 -
microtubule-based movement [Evidence IEA]; GO_process:
GO:0051228 - mitotic spindle disassembly [Evidence IMP]
[PMID 23851487]; GO_process: GO:0051228 - mitotic spindle
disassembly [Evidence IMP] [PMID 9813090]; GO_process:
GO:0030472 - mitotic spindle organization in nucleus
[Evidence IGI,IMP] [PMID 10525539]; GO_process: GO:0031115
- negative regulation of microtubule polymerization
[Evidence IMP] [PMID 24616221]; GO_process: GO:0030473 -
nuclear migration along microtubule [Evidence IMP] [PMID
9693366]; GO_process: GO:0070462 - plus-end specific
microtubule depolymerization [Evidence IDA] [PMID
16906145]; GO_process: GO:0070462 - plus-end specific
microtubule depolymerization [Evidence IDA] [PMID
16906148]; GO_process: GO:0032888 - regulation of mitotic
spindle elongation [Evidence IMP] [PMID 24616221];
GO_process: GO:0090307 - spindle assembly involved in
mitosis [Evidence IMP] [PMID 23851487]"
/codon_start=1
/product="tubulin-dependent ATPase KIP3"
/protein_id="XP_018736991.1"
/db_xref="GeneID:30033984"
/translation="MQQALFNGGGGNSNGSGSSSSNGMRQNRIREHRFVFDRLFDDTA
TQNDVYCHTTRPLLDNVLDGYNATVFAYGATGCGKTHTISGNPSQPGIIFLTMKELFE
RIDALRDTKTIDLSLSYLEIYNETIRDLLEPAGSGPGPKRILQLRENADKRISVSNLS
SHKPENVDQVMDMILQGNQNRTMSPTEANAVSSRSHAVLQINITQKARTAELSEAHTF
ATLSIIDLAGSERASATKNRGERLIEGANINKSLLALGNCINALCDPRQRGHVPYRDS
KLTRLLKFSLGGNCKTVMIVCVSPSSQHYDETLNTLKYADRAKKIKTKVSRNQHSLDR
HVGSYLKMITEQRAEIEELRNREAKVIQEALDKDRMTREKRLKALEEALESLDAAVEK
SRSEYSNKSVCISKLRTLSLQISQIDAIAEALVENSKQYQLPGMEVFQSALQMAKNKA
TAEQGKIQQYLQSINASQAIDSAATILSRRLQETSGWSKQDSTNFENYLNWKKSSLEN
ELLQKSLTGTPSAISLNVVARLLFKCIGRLESLRSQPGEIIAGEVTLLTKELLNDMER
SNILTEPTGCESNAFDLSLLNQPQQPLLHTSSVMNNVVHMTASPKRLSSSRRVSILKK
KSPRLLRRSSVLMRASSPLKPRKTSKRVRWEDSEDTDSLANDMTESASDMGLSDSSMS
VDRSTPTVAPLTDPRVTRLMSPLREVTLDSDINSSIDYSVSHPSGMGKKIGPRSDLTR
RSLNKPRPSILGINKHAPGRVEKENSSSPISSLDNQSILLSPSSTPPVPTLSSRSSGT
PFHLAATASSSMKQNLRRS"
gene <423686..>425777
/gene="OXA1"
/locus_tag="AWJ20_2110"
/db_xref="GeneID:30033986"
mRNA join(<423686..423807,424616..>425777)
/gene="OXA1"
/locus_tag="AWJ20_2110"
/product="Oxa1p"
/transcript_id="XM_018879038.1"
/db_xref="GeneID:30033986"
CDS join(423686..423807,424616..425777)
/gene="OXA1"
/locus_tag="AWJ20_2110"
/inference="similar to AA sequence:KEGG_Orthology:K03217"
/note="Mitochondrial inner membrane insertase; mediates
the insertion of both mitochondrial- and nuclear-encoded
proteins from the matrix into the inner membrane; also has
a role in insertion of carrier proteins into the inner
membrane; acts as a voltage-gated ion channel, activated
by substrate peptides; interacts with mitochondrial
ribosomes; conserved from bacteria to animals;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA,IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0030061 - mitochondrial crista [Evidence
IDA] [PMID 22513091]; GO_component: GO:0097002 -
mitochondrial inner boundary membrane [Evidence IDA] [PMID
22513091]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IEA,IEA]; GO_component: GO:0005762 -
mitochondrial large ribosomal subunit [Evidence IPI] [PMID
14657017]; GO_component: GO:0005762 - mitochondrial large
ribosomal subunit [Evidence IPI] [PMID 19783770];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0005216 - ion channel
activity [Evidence IDA] [PMID 22829595]; GO_function:
GO:0032977 - membrane insertase activity [Evidence IMP]
[PMID 9285818]; GO_function: GO:0032977 - membrane
insertase activity [Evidence IMP] [PMID 9428747];
GO_function: GO:0097177 - mitochondrial ribosome binding
[Evidence IDA] [PMID 14657018]; GO_process: GO:0000002 -
mitochondrial genome maintenance [Evidence IGI,IMP] [PMID
18826960]; GO_process: GO:0033615 - mitochondrial
proton-transporting ATP synthase complex assembly
[Evidence IMP] [PMID 17344477]; GO_process: GO:0045039 -
protein import into mitochondrial inner membrane [Evidence
IMP] [PMID 22846909]; GO_process: GO:0051205 - protein
insertion into membrane [Evidence IEA]; GO_process:
GO:0032979 - protein insertion into mitochondrial membrane
from inner side [Evidence IMP] [PMID 17452441];
GO_process: GO:0032979 - protein insertion into
mitochondrial membrane from inner side [Evidence IMP]
[PMID 9285818]; GO_process: GO:0032979 - protein insertion
into mitochondrial membrane from inner side [Evidence IMP]
[PMID 9428747]; GO_process: GO:0032979 - protein insertion
into mitochondrial membrane from inner side [Evidence
IMP,IPI] [PMID 9482871]"
/codon_start=1
/product="Oxa1p"
/protein_id="XP_018736992.1"
/db_xref="GeneID:30033986"
/translation="MLLREDTLPDIGLATFETNPEPKSTVASPEPRTGEAEDDEPYGS
SVLQQSVFGVRPALRQTKAFPGFAKRFNSSQVASATTSSTNGTTETVSELAASAGSAI
QTEIAPIVQSASETIQAVVDTVSPDQIGYFQSVGLAQSWWWPPGMFQHIFEIVHVSTG
LPWWATIAMVTVGMRAVMFPMYLKASNATAKMTALKPELNRLMKDYTNSDDPIAAQKT
LMERKKLMLAHNVKTSHLLLPMASVPFFIGVFSALNGMSKVPVHGLTSEGALWFTNLA
AADPYVGLQLITALFYATTFKLGGETGANTMSPAMKKIFTYMPLIAVPMTMSLPASVC
MYFALNGVLSVGQTFLLQNKTFRNKVGLAPIVKPAMNPEDNLSIMETLKQSFEKTKQR
AEKAQREAERELRLKKEKEAEERSKYVQFTKKPRK"
gene complement(<425996..>427117)
/gene="LPX1"
/locus_tag="AWJ20_2111"
/db_xref="GeneID:30033987"
mRNA complement(<425996..>427117)
/gene="LPX1"
/locus_tag="AWJ20_2111"
/product="Lpx1p"
/transcript_id="XM_018879039.1"
/db_xref="GeneID:30033987"
CDS complement(425996..427117)
/gene="LPX1"
/locus_tag="AWJ20_2111"
/note="Peroxisomal matrix-localized lipase; required for
normal peroxisome morphology; contains a peroxisomal
targeting signal type 1 (PTS1) and a lipase motif;
peroxisomal import requires the PTS1 receptor, Pex5p and
self-interaction; transcriptionally activated by Yrm1p
along with genes involved in multidrug resistance; oleic
acid inducible; GO_component: GO:0005782 - peroxisomal
matrix [Evidence IEA]; GO_component: GO:0005782 -
peroxisomal matrix [Evidence IDA] [PMID 12135984];
GO_component: GO:0005777 - peroxisome [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016298 - lipase activity [Evidence
IDA] [PMID 18199283]; GO_function: GO:0016298 - lipase
activity [Evidence ISS] [PMID 9200815]; GO_function:
GO:0004806 - triglyceride lipase activity [Evidence IDA]
[PMID 24187129]; GO_process: GO:0007031 - peroxisome
organization [Evidence IEP] [PMID 12135984]; GO_process:
GO:0019433 - triglyceride catabolic process [Evidence IGI]
[PMID 24187129]"
/codon_start=1
/product="Lpx1p"
/protein_id="XP_018736993.1"
/db_xref="GeneID:30033987"
/translation="MTITKERFVIDAHHPRESFDSTVSPDDRLKLVYNVYKDDRFDNS
TADPTTLINLVFAHGTQMNKEIWQYMIELFYNDFGARLGNVVAIDAINHGESLILNNG
KLGPYITWSDGGKDINCVIRALNNVGPTILIGQSMGGAMCTYAVIHEPGLIDSIVIID
PVLYLDPEYYTNEHVTALTSDRFKKLEKYIKTKFQNREEYETYMSKKTISRGFHPRVL
KDYIDTSAIENPDGTISTINSLDQQLISYLSSQPTLRDLYSLLTAIDSEVLYVCGDIA
DWNPPDAHEKVIERLRYGSLKIIPGGKHLVVFDMPDETYAAIKDFVDRRGKRGSELIQ
KARARSNYSLNQRLEFAHEGRKYQLESLAAGKRFVYSKL"
gene <429657..>431081
/gene="GAL83"
/locus_tag="AWJ20_2112"
/db_xref="GeneID:30033988"
mRNA <429657..>431081
/gene="GAL83"
/locus_tag="AWJ20_2112"
/product="Gal83p"
/transcript_id="XM_018879040.1"
/db_xref="GeneID:30033988"
CDS 429657..431081
/gene="GAL83"
/locus_tag="AWJ20_2112"
/note="One of three possible beta-subunits of the Snf1
kinase complex; allows nuclear localization of the Snf1
kinase complex in the presence of a nonfermentable carbon
source; contains glycogen-binding domain; GAL83 has a
paralog, SIP2, that arose from the whole genome
duplication; GO_component: GO:0031588 - AMP-activated
protein kinase complex [Evidence IDA] [PMID 12393914];
GO_component: GO:0031588 - AMP-activated protein kinase
complex [Evidence IPI] [PMID 7813428]; GO_component:
GO:0031588 - AMP-activated protein kinase complex
[Evidence IPI] [PMID 9121458]; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 17237508]; GO_component:
GO:0005641 - nuclear envelope lumen [Evidence IDA] [PMID
17237508]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 11331606]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 17237508]; GO_function: GO:0004679 -
AMP-activated protein kinase activity [Evidence IGI] [PMID
10990457]; GO_function: GO:0004679 - AMP-activated protein
kinase activity [Evidence IMP] [PMID 7813428]; GO_process:
GO:0007155 - cell adhesion [Evidence IMP] [PMID 12556493];
GO_process: GO:0001403 - invasive growth in response to
glucose limitation [Evidence IMP] [PMID 12556493];
GO_process: GO:0006468 - protein phosphorylation [Evidence
IGI] [PMID 10990457]; GO_process: GO:0043254 - regulation
of protein complex assembly [Evidence IGI] [PMID 9121458];
GO_process: GO:0007165 - signal transduction [Evidence
IGI] [PMID 10990457]"
/codon_start=1
/product="Gal83p"
/protein_id="XP_018736994.1"
/db_xref="GeneID:30033988"
/translation="MGNSSSQEDDAANKQQQQQHQQQQHHRHHFHHSHHKGSNTSTPS
HDQEEFPPVPPQGFDQIPIAGVGVTATTSVSKEQATVSPPGGTSGNTGSLNQHQRSSS
FHHEEFASASHEENVRQVPATATTPSSATTAQPPAASAGVGGSNKPVVQPLQSYDANS
TNSNDGAKRPPLRRKSTLLLDEPAEMDEEEMELSKLKLTNELKPLSRAGSGEEVVLTN
QDDYQGNVFSTVIDWKQGGNKVYVTGTFTGWRKMIRLSKVSEEQLQNQKAHADASGDN
QQPPLASTPPTISKDLFSVVLKLPKGTHRLRFVVDNELRCSDFLPTATDSMGNLVNYI
EVGVEEDRPVQSQSQAVAGGIGGDDEGDGEYEKFNEDDEMLREPVLQYGNEIPSVFTD
PEVMDQFVSSDFVTPPQLPPHLEGVILNSNSTEKDNNSILPIPNHVVLNHLATTSIKH
NVLAVASISRYSRKYVTQILYAPL"
gene <435444..>441317
/gene="BEM2"
/locus_tag="AWJ20_2113"
/db_xref="GeneID:30033989"
mRNA <435444..>441317
/gene="BEM2"
/locus_tag="AWJ20_2113"
/product="Bem2p"
/transcript_id="XM_018879041.1"
/db_xref="GeneID:30033989"
CDS 435444..441317
/gene="BEM2"
/locus_tag="AWJ20_2113"
/note="Rho GTPase activating protein (RhoGAP); involved in
the control of cytoskeleton organization and cellular
morphogenesis; required for bud emergence; potential GAP
for Rho4p; GO_component: GO:0005938 - cell cortex
[Evidence IDA] [PMID 17914457]; GO_component: GO:0005934 -
cellular bud tip [Evidence IDA] [PMID 17914457];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
17914457]; GO_component: GO:0000131 - incipient cellular
bud site [Evidence IDA] [PMID 17914457]; GO_component:
GO:0005622 - intracellular [Evidence IEA]; GO_component:
GO:0043332 - mating projection tip [Evidence IDA] [PMID
17914457]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 17914457]; GO_component: GO:0005628 - prospore
membrane [Evidence IDA] [PMID 24390141]; GO_function:
GO:0005096 - GTPase activator activity [Evidence IEA];
GO_function: GO:0005100 - Rho GTPase activator activity
[Evidence IDA] [PMID 7962098]; GO_function: GO:0005085 -
guanyl-nucleotide exchange factor activity [Evidence IEA];
GO_function: GO:0003729 - mRNA binding [Evidence IDA]
[PMID 23222640]; GO_process: GO:0030036 - actin
cytoskeleton organization [Evidence IGI] [PMID 7579704];
GO_process: GO:0030010 - establishment of cell polarity
[Evidence IC] [PMID 7579704]; GO_process: GO:0035024 -
negative regulation of Rho protein signal transduction
[Evidence IGI,IMP] [PMID 17914457]; GO_process: GO:0043547
- positive regulation of GTPase activity [Evidence IEA];
GO_process: GO:0043087 - regulation of GTPase activity
[Evidence IEA]; GO_process: GO:0051056 - regulation of
small GTPase mediated signal transduction [Evidence IEA];
GO_process: GO:0007165 - signal transduction [Evidence
IEA]; GO_process: GO:0007264 - small GTPase mediated
signal transduction [Evidence IEA]"
/codon_start=1
/product="Bem2p"
/protein_id="XP_018736995.1"
/db_xref="GeneID:30033989"
/translation="MERRTQALRGGSAAGGSDGLNAAKDGWVNRHASSASATTAVAGS
GSGAGISSSASSNTLVPSGSISSNLTALASGPGGSGGTNGAAVTSQVYSWRLTRAVLK
NGTLLLYKPPTDLGIKSFDPSLEPAHPLSPNLSSPTSATSGGGGHKRVGSVSTATAAS
SSSASVSAAAAAAGSRSFHSLSYRGFDVHPDLLFDSQGKIVNGSFEAICHSILFTGDE
DFATTAVLTIPLMGDVVRALEMMKEYVLSVARQAHSGGGGPNAQAIAVRVKLVIDTML
TNFKGMLLNNSIHASLQQLADSVSSYNDQISTALKLSIYQKHQRMNKMLTYTREDTEV
LWSMQQKVTDSMSERLHAFLGRIDSSIGSDSISSIANLPPRQQVAHNLSGSGSGGGSG
GTGGVGGGLSAQQLNIPPDLFIELTSTDFLANQISCFHLKFYKQWSPAIDISLLFKTN
YSYHRYNPLVFDANNLHFLGVLLIDHLLSPKYSKLFDNSYRGRLLTHWINLGNSLKVC
GDLVGWVAIVTAICSPAILRLRECWAYVPIELRENVGKDWGHELFELDRRAKMDLMSK
RTFRVATEDIGVTYPKERSISFFADLTVKIDEGATSYKMCEDRVNRVRTVINSWKYFF
DRIPQNDTFGLPPQPNAAVQKLLHSLLVTNYETPQLTQEEIFKLSLSVDPPKFGNYLK
YHYSQQSPLMTGSFLPLIFTDTNATYKLFTKSSLISALSGVAGTGGASALSSSNKRLV
RSGTTRSSDGTNASTIPTSASSNFYTHGGHSTLSTSSTLLSGGRNASTNLHRSNSFPP
SNTKSSAMTTGVQDIDYFSREFVAKYASQHILMKNIRDVLNMGAKLYHINEDIVLKSL
SEEYNSRPSSMIEAPSKHLSMSSRRVSTQFGSTVSPRTSVNGGTPDFSKVFESFAVSI
DVVVKSANLERLVDILVLGVNGFASFVNQQDVDRQGNLPDFRIDMNVHTLTFFATFRS
FCSPLELLTSLRKRFVGARSAAMSIDELTDKEREVAFDDVQFPNWDPVCEIPLDNNRA
WGIVAQIQIGVLEACHLWVSQYFSDFADNVSLKEQFLELTEIFGTDIASWKGLQASSQ
INEDYSGYLETMEVLQKKVRNLFYKKSYRPNDVCPLIPQNPVGMRYENLPVGNTNVTF
MKLEKFIDDINLVVSEYLGMVSVKEWMELFETLELQSAEINGFFHYSPPPYSSEDDFV
IQDIFTYLESLYRENANGERAKTLDFFPPSIQELFRLRHNIISYLTYQIGDPLIHKDD
RIQRMISILRMIGISKARMTFLDLFPKDETTDSDQLWMSQADNSGISTSVPSFIESCV
IASVLRPESRLFANSWMQASREICKLYHRSSNPTSFASLEMFVPVIRGENLVFSGSKQ
PLSPCVGWTIERMFEIVCYVPNMSVENSSLINFDKRRFIYNLITNVSEMKGEIEDDNE
DGSRVSNGSHRIQTVARRIAYIVNPDKTLYYTDKRTLKESVARDTKEYPRSYSKNKVF
TSYVTLEAEKLKRDVRQRELIERQYRDVKKSLKSRYGSSSSSSGTIGSQTLSDKKSQR
SRFGGLFKAVRPISMAFSGTFIPPSDRIVSVYELPDISTISDSKCKLLASINLANCDI
IAVPNQHGNYGLFKVVVQDNNGSELVFQGLSDEDAAEWVRLATTGKRYATLRGQLQPS
SSSSSPNAPASPVAFTRVFGVPIAVVCEREARDIPMVVDVLLNEIESRGLDEVGLYRV
SGSLASVHALKAAFDSGQEVDMEDDRWFDINTVAGCFKLYLRELREPLLTTELFPEFV
QCGSSGAGTEDMLKQLRVSIRKLPAPNYNLLRRLLRHLGQVAEHEANNKMKTPNLAIV
FSMSFLPASAVDQMKAMQNVLNTMIIHHERLFGDDQPVEYSTEAAEITANTADLSLGE
TTNSHDQNHNTASDNQPDSSEPSTTHSGTENTDSQLPTGNQAGSKHDRDSFTEVSAY"
gene <442341..>443831
/gene="MDM34"
/locus_tag="AWJ20_2114"
/db_xref="GeneID:30033990"
mRNA <442341..>443831
/gene="MDM34"
/locus_tag="AWJ20_2114"
/product="ERMES complex subunit MDM34"
/transcript_id="XM_018879042.1"
/db_xref="GeneID:30033990"
CDS 442341..443831
/gene="MDM34"
/locus_tag="AWJ20_2114"
/inference="similar to AA sequence:KEGG_Orthology:K17775"
/codon_start=1
/product="ERMES complex subunit MDM34"
/protein_id="XP_018736996.1"
/db_xref="GeneID:30033990"
/translation="MSFKFNWNSFNDESFYTRAKKLLTDALNKSNKPPIIVDKIIVQD
LSLGSEAPDLEILEIGDLADDRFRGIFKLTYSGNASITLATKVQANPLNVYAQNAPGF
CMPQFLGASSSLAMPLNLTLSDIVLSGIIILVFSKAKGLTLVFRNDPLEKIKVSSTFD
ALPGIAKFLQVQIENQIRVLFREELPTILHKLSQKWTPSGSEMSELMSLKSHTSSPIS
TEPSSPRLKPVSLMEINPDMPEFSPTNMLKLEALCASQRTLNLFTPAIPEAVYRSSLE
SFENARRKNGRDVQSLVGGDDLYDVMRLQTKQAQNRSSNITPKRRVIKIKRNKASDST
NSDTPSAEASAASTPISSSTPTSASTATSASSNTTSSTNTSSSLSPSSSNTSSNSSAD
LNSSKTTSIAFLPVIDESLASTSAIPSLDLKLPRQRRESILKPTSILEQDGKQAPSSK
HEHEHVHWTHENALPKDKLFKDFFSRNVNIRPDDHTNNPPPPAYVA"
gene complement(<444091..>445053)
/locus_tag="AWJ20_2115"
/db_xref="GeneID:30033991"
mRNA complement(<444091..>445053)
/locus_tag="AWJ20_2115"
/product="hypothetical protein"
/transcript_id="XM_018879043.1"
/db_xref="GeneID:30033991"
CDS complement(444091..445053)
/locus_tag="AWJ20_2115"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018736997.1"
/db_xref="GeneID:30033991"
/translation="MKIGTHSGTFHADESLAVYMLRLLPRFKDAELLRSRDNEQLETC
DIIVDVGGKYEAPKYFDHHQREFNDTFDDEHKTRLSSAGLIYKHFGKEIIAQLTEVSV
DDASIDVLYNKIYNEFIEAIDANDNGISAYPEDTPSSYRTGGITLPSLVSKLNPLWTE
PNTDEVLDAKFLKASELMGTAFVSVVENYGKGWYPAKQHVQAAFDARDQYGSEGRIVV
FDTFVPWKEHLYTIEKELGKEGEVLYVLYSDGKGWRIQAVAQSSSSFTSRKPLPEPWR
GARDEKMSEITGVPGCVFTHVSGFIGGHKTREGALELAKLALQA"
gene complement(<445482..>448082)
/locus_tag="AWJ20_2116"
/db_xref="GeneID:30033992"
mRNA complement(<445482..>448082)
/locus_tag="AWJ20_2116"
/product="putative carboxylic ester hydrolase"
/transcript_id="XM_018879044.1"
/db_xref="GeneID:30033992"
CDS complement(445482..448082)
/locus_tag="AWJ20_2116"
/note="Putative acyltransferase with similarity to Eeb1p
and Eht1p; has a minor role in medium-chain fatty acid
ethyl ester biosynthesis; may be involved in lipid
metabolism and detoxification; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0016787
- hydrolase activity [Evidence IEA]; GO_function:
GO:0016746 - transferase activity, transferring acyl
groups [Evidence ISS] [PMID 16361250]; GO_process:
GO:0051792 - medium-chain fatty acid biosynthetic process
[Evidence IGI] [PMID 16361250]"
/codon_start=1
/product="putative carboxylic ester hydrolase"
/protein_id="XP_018736998.1"
/db_xref="GeneID:30033992"
/translation="MVEQEAPARELWFFHDSPVELEDPSTPLPTATATPPQGTPTQSP
RRSREKSPATFGAHFSESLRSRGRERSVSTGTPVNGTSGNTLKPRPSSGSLKDEESQN
TPAKGSISYPVFSVTSLVGSVTPNLNWQAFSPYDCRLLNKEYNKPLEEEEEPVPVGCN
RLYQVSLQSMDMTPIYWAPLTSPYNVHCSSWFFSTSLTPIEPSLEDAIEEAFQEIKPW
TSTYLAELEAAITVPEAMTKLKTPITYFDGRSDVSVEVVFAPTFKRDSANELQPEQEE
EEKEEEEEEDSNDKKGQDIDLAKGESEKNDQTAKEEDGAKDVNSDAASIASSVASGPE
SYPVAYIFSASSSVSIPYIFSSTSPAQLISSLVAGKAPPGTLFTVQRHFSWQDWKALR
NLPDRPSDGDEYAPRKITQLVLVFHGIGQKLTERVESFNFTYAINSFRIQVSNFVDSQ
QLSQYIKEDTNILTLPINWRRIIDFENLKGTENKPKNSEFSLSDITMKSIPSVRNLIS
DVMLDIPYYMSRHKKLLLDAAAQEANRVYSLFTKINPEFEKSGGTVNIIGHSLGSSIA
VDLLSSQPTDVTKCKPEELEKKRTLKFNVHNLFLVGSPVGFFLLLENAQLHPRELYDR
KNKIDPGDTTHKYPYGCIAVRNVYNILHYSDPIAYYIGPTVDRLQVSLIETATLPSEK
AFIVAQAADAAGWVNALTSRIPFMGSPKNESTKDITIQEKEKQMPDEEDAEVQILDEK
TKPLKELLPGVTLNSEYVEVEPVTNPTDAQLPKPGQTVELEDRDFEKESSGEEKLILL
NENGQIDWVIPLTVSLESVSQYVSMLTAHSGYWDSKDFARMVAIECGRAKGVDNTISQ
YRARKKNKSS"
gene <450004..>451929
/gene="LSC1"
/locus_tag="AWJ20_2117"
/db_xref="GeneID:30033993"
mRNA <450004..>451929
/gene="LSC1"
/locus_tag="AWJ20_2117"
/product="succinate--CoA ligase (GDP-forming) subunit
alpha"
/transcript_id="XM_018879045.1"
/db_xref="GeneID:30033993"
CDS 450004..451929
/gene="LSC1"
/locus_tag="AWJ20_2117"
/inference="similar to AA sequence:KEGG_Orthology:K01648"
/note="Alpha subunit of succinyl-CoA ligase; succinyl-CoA
ligase is a mitochondrial enzyme of the TCA cycle that
catalyzes the nucleotide-dependent conversion of
succinyl-CoA to succinate; phosphorylated; GO_component:
GO:0042645 - mitochondrial nucleoid [Evidence IDA] [PMID
15692048]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 9874242]; GO_function: GO:0005524 -
ATP binding [Evidence IEA]; GO_function: GO:0003878 - ATP
citrate synthase activity [Evidence IEA]; GO_function:
GO:0005525 - GTP binding [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0048037 - cofactor binding [Evidence IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0004775 - succinate-CoA ligase
(ADP-forming) activity [Evidence IEA,IEA]; GO_function:
GO:0004775 - succinate-CoA ligase (ADP-forming) activity
[Evidence IDA,IMP,ISS] [PMID 9874242]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0006104 - succinyl-CoA metabolic process [Evidence TAS]
[PMID 9175438]; GO_process: GO:0006104 - succinyl-CoA
metabolic process [Evidence IDA] [PMID 9874242];
GO_process: GO:0006099 - tricarboxylic acid cycle
[Evidence IEA,IEA]; GO_process: GO:0006099 - tricarboxylic
acid cycle [Evidence TAS] [PMID 9175438]"
/codon_start=1
/product="succinate--CoA ligase (GDP-forming) subunit
alpha"
/protein_id="XP_018736999.1"
/db_xref="GeneID:30033993"
/translation="MSLNGTAPPAYELFSNKTRAFVYGLQPRACQGMLDFDFICKRDV
PSVAGVIYPFGGQFVTKMYWGTKETLLPVYQQVEKAAAKHPEVDVVVNFASSRSVYSS
TMELLEYPQFRTIAIIAEGVPERRAREILYKAQKKGVTIIGPATVGGIKPGCFKIGNT
GGMMDNIVASKLYRPGSVAYVSKSGGMSNELNNIIAQTTNGVYEGVAIGGDRYPGTTF
IDHILRYQADPNCKIIALLGEVGGVEEYRVIEAVKSGKITKPIVAWAIGTCASMFKTE
VQFGHAGSMANSDLETATAKNAAMKAAGFHVPDTFEDFPETLAQVYETLVKKGVIVPQ
PEPEVPKIPIDYSWAQELGLIRKPAAFISTISDDRGQELLYAGIPISDVFKDDIGIGG
VMSLLWFRRRLPAYASKFLEMVLMLTADHGPAVSGAMNTIITTRAGKDLISSLVSGLL
TIGTRFGGALDGAATEFTNAFDKGLSPRQFVDTMRKQNKLIPGIGHKVKSRNNPDFRV
ELVKDFVKKNFPSTTLLDYALAVEEVTTSKKDNLILNVDGAIAVSFIDLMRSSGAFTP
EEVDEYLKNGVLNGLFVLGRSIGLIAHHLDQKRLKTGLYRHPWDDITYLVGQDAVSSA
NKRVEVDAKGVSKAKSK"
gene complement(<452244..>453203)
/gene="GTR2"
/locus_tag="AWJ20_2118"
/db_xref="GeneID:30033994"
mRNA complement(<452244..>453203)
/gene="GTR2"
/locus_tag="AWJ20_2118"
/product="Gtr2p"
/transcript_id="XM_018879046.1"
/db_xref="GeneID:30033994"
CDS complement(452244..453203)
/gene="GTR2"
/locus_tag="AWJ20_2118"
/inference="similar to AA sequence:KEGG_Orthology:K16186"
/note="Putative GTP binding protein; negatively regulates
Ran/Tc4 GTPase cycle; activates transcription; subunit of
EGO and GSE complexes; required for sorting of Gap1p;
localizes to cytoplasm and to chromatin; homolog of human
RagC and RagD; GO_component: GO:0034448 - EGO complex
[Evidence IPI] [PMID 15989961]; GO_component: GO:0034448 -
EGO complex [Evidence IDA] [PMID 16732272]; GO_component:
GO:1990131 - Gtr1-Gtr2 GTPase complex [Evidence IPI] [PMID
10388807]; GO_component: GO:1990131 - Gtr1-Gtr2 GTPase
complex [Evidence IPI] [PMID 16143306]; GO_component:
GO:0005737 - cytoplasm [Evidence IEA]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 10388807];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
11073942]; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 15989961]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0031902 - late endosome
membrane [Evidence IDA] [PMID 16732272]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_component:
GO:0000790 - nuclear chromatin [Evidence IDA] [PMID
18258182]; GO_component: GO:0005634 - nucleus [Evidence
IEA]; GO_component: GO:0005634 - nucleus [Evidence IDA]
[PMID 10388807]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 11073942]; GO_component: GO:0005774 -
vacuolar membrane [Evidence IEA]; GO_component: GO:0005773
- vacuole [Evidence IEA]; GO_function: GO:0005525 - GTP
binding [Evidence IEA,IEA]; GO_function: GO:0005525 - GTP
binding [Evidence ISA] [PMID 10388807]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0006914 - autophagy [Evidence IEA];
GO_process: GO:0016237 - microautophagy [Evidence IMP]
[PMID 15989961]; GO_process: GO:0032008 - positive
regulation of TOR signaling [Evidence IGI,IMP] [PMID
19748353]; GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IDA] [PMID 18258182]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Gtr2p"
/protein_id="XP_018737000.1"
/db_xref="GeneID:30033994"
/translation="MQPLETLYIESTAKPTYERFDSLVNFAVQELPGQLDMFEPSYDS
ERIFAQVGSLIYVIDSQDEYLYALQNLQQIIEYAFKINPAINFEVLIHKVDGLSDDFR
LDTQRDIMQRVTDELVDANLEGVNIAFHLTSIFDHSIYEAFSRIIQKLVPELPVLENL
LDILCQHSGIEKAFLFDVNSKIYLATDSSPVDVETYQVCSDFIDVAVDLDLLYKPSYT
QQQQQPQTQQTPESNNSPGLAAVTNGSGPSQTPEGPGLKCTSKLHNGMVLYLSQMIRG
LALVGMIRSEGVQKMTLVDYNVEIFRQGLTRVYAEDPLHPHPA"
gene <454004..>454867
/gene="TYW3"
/locus_tag="AWJ20_2119"
/db_xref="GeneID:30033995"
mRNA <454004..>454867
/gene="TYW3"
/locus_tag="AWJ20_2119"
/product="Tyw3p"
/transcript_id="XM_018879047.1"
/db_xref="GeneID:30033995"
CDS 454004..454867
/gene="TYW3"
/locus_tag="AWJ20_2119"
/inference="similar to AA sequence:KEGG_Orthology:K15450"
/note="tRNA methyltransferase required for synthesis of
wybutosine; a modified guanosine found at the 3'-position
adjacent to the anticodon of phenylalanine tRNA which
supports reading frame maintenance by stabilizing
codon-anticodon interactions; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0008168
- methyltransferase activity [Evidence IEA]; GO_function:
GO:0008175 - tRNA methyltransferase activity [Evidence
IDA,IMP] [PMID 16642040]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0032259 - methylation [Evidence IEA]; GO_process:
GO:0030488 - tRNA methylation [Evidence IDA,IMP] [PMID
16642040]; GO_process: GO:0008033 - tRNA processing
[Evidence IEA]; GO_process: GO:0031591 - wybutosine
biosynthetic process [Evidence IMP] [PMID 16642040];
GO_process: GO:0031591 - wybutosine biosynthetic process
[Evidence IMP] [PMID 17150819]"
/codon_start=1
/product="Tyw3p"
/protein_id="XP_018737001.1"
/db_xref="GeneID:30033995"
/translation="MRESDSEEMSQNPFDQKKRAILSQIESTDETHPDASPKGTLDVL
LLPLIDVINSHSDYVTTSSCSGRVSVFLEGMKTASELGGKGDGGKWLFITHDPEELKS
TDWWSLVTDHREHVSESDVSAGVSSIGNGEPAKYDGQRYVLYKFEAMILHVKCRDRAS
ASALYTAAMSCGFRESGIGSNDLVGIRISLRLDVPIAYLNNDGTTTPLVPRQYIDHLQ
TISLDLFNKNTAKINQLHRTVASMIQAQQTAANKPQTAKETKEQRRERKHREGLLIQA
QTRQITPEAPL"
gene <455207..>456886
/gene="DPS1"
/locus_tag="AWJ20_2120"
/db_xref="GeneID:30033997"
mRNA <455207..>456886
/gene="DPS1"
/locus_tag="AWJ20_2120"
/product="aspartate--tRNA ligase DPS1"
/transcript_id="XM_018879049.1"
/db_xref="GeneID:30033997"
CDS 455207..456886
/gene="DPS1"
/locus_tag="AWJ20_2120"
/inference="similar to AA sequence:KEGG_Orthology:K01876"
/note="Aspartyl-tRNA synthetase, primarily cytoplasmic;
homodimeric enzyme that catalyzes the specific
aspartylation of tRNA(Asp); class II aminoacyl tRNA
synthetase; binding to its own mRNA may confer
autoregulation; shares five highly conserved amino acids
with human that when mutated cause leukoencephalopathy
characterized by hypomyelination with brain stem and
spinal cord involvement and leg spasticity (HBSL);
GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 16113655]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 16113655]; GO_function:
GO:0005524 - ATP binding [Evidence IEA,IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IDA] [PMID 12888345];
GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
[Evidence IEA,IEA]; GO_function: GO:0004815 -
aspartate-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0004815 - aspartate-tRNA ligase activity
[Evidence IDA] [PMID 8263943]; GO_function: GO:0016874 -
ligase activity [Evidence IEA]; GO_function: GO:0003676 -
nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA,IEA];
GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
[Evidence IEA]; GO_process: GO:0006422 - aspartyl-tRNA
aminoacylation [Evidence IDA] [PMID 8263943]; GO_process:
GO:0006418 - tRNA aminoacylation for protein translation
[Evidence IEA]; GO_process: GO:0006412 - translation
[Evidence IEA]"
/codon_start=1
/product="aspartate--tRNA ligase DPS1"
/protein_id="XP_018737002.1"
/db_xref="GeneID:30033997"
/translation="MSEEIVKKTEELKIEEGSAAAAGAPAGEVVLGADGKPLSKSALK
KLEKERAKEEKKRQIAERLAAEKAAREASEVDYSKEFYGKQPLNQSGEKTGEQRVQIS
NIDKSWAGKEVIFRARVHNSRMQSSKMCFMSLRQQDAVIQALLVVDSDTISKQMVKWA
GSINLESIVVVYGKVEASPEEISSATVKDAEIKITKIFTIAETPAQLPILIEDASRSE
AEAKEVGLPYVNLDTRLDARVIDLRTATNQAIFKISSGVCELFREFLIKKGFVEIHTP
KIIPAASEGGANVFKVNYFKGEAFLAQSPQLYKQMLIAADFEKVFEIAPVFRAENSNT
HRHMTEFVGLDLEMAFEEHYDEVIDVLVEMFMFIFTELKNRYAKEIALVRKQYPVEEF
KLPKDGKMVRLHFKEGIQLLREVGGVDVPDLEDLSTEHEKLLGKIVREKYDTDFYILD
KFPLAVRPFYTMPDAADSNYSNSYDFFMRGEEILSGAQRIHDANFLKTRMAAHEVDSN
TVKDYVDAFSYGAPPHAGGGIGLERVVMFFLDLKNIRRASLFPRDPKRVRP"
gene <460693..>461982
/locus_tag="AWJ20_2121"
/db_xref="GeneID:30033998"
mRNA <460693..>461982
/locus_tag="AWJ20_2121"
/product="hypothetical protein"
/transcript_id="XM_018879050.1"
/db_xref="GeneID:30033998"
CDS 460693..461982
/locus_tag="AWJ20_2121"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737003.1"
/db_xref="GeneID:30033998"
/translation="MEKFNLDFRPDFKRDVKPDFKPDFKIGEKQQSPPISTQFVQSPS
SHSHLNRSSSSSPSSTVSSNTDQHSNNSNNSNNSSSNRLVRVIDTVVALYSYQSDKPF
SLSFKKGDNIYVISKLPSGWWEGVTPSGSQGWFPSNYTQVVPGAQQNQLLFDSRPSIA
SHTSNDSMDPLAPATLADDDPWVSNLSSSTTSAHSSSASYARSNSIGGMTGTGIPLTT
TLSSNSGIYTGSRKGSVTSYESSITEELTHNAAATDNGFTATKSTNAPCESYTRVNTS
SDTYNYPPSFWLPQINNSGKLIFMNAGRLHVSSQIPWEKVDVNSLDKEIIYPNSLTVP
QDLIQAPRVPTSISEGNRLFEKSVDPYADLVSFLWPPRCLRRLGLRPRPWLLLSLRSS
RCVDEPSLSCEAGATRVWGGAPAAGGMCPSCTMLTRL"
gene <462016..>464856
/gene="CDC25"
/locus_tag="AWJ20_2122"
/db_xref="GeneID:30033999"
mRNA <462016..>464856
/gene="CDC25"
/locus_tag="AWJ20_2122"
/product="Ras family guanine nucleotide exchange factor
CDC25"
/transcript_id="XM_018879051.1"
/db_xref="GeneID:30033999"
CDS 462016..464856
/gene="CDC25"
/locus_tag="AWJ20_2122"
/inference="similar to AA sequence:KEGG_Orthology:K03099"
/codon_start=1
/product="Ras family guanine nucleotide exchange factor
CDC25"
/protein_id="XP_018737004.1"
/db_xref="GeneID:30033999"
/translation="MDYTSISLETSLMTLQEFSDEDFEETWPEIIAKLEKSASKLESH
VSPDEEYEFVMGFADLAGIIQKIALYVGVPPGAFPSSGNEFAGISLAFRRMINTLSRI
SISHSIGSMIRHQSVGSLEEPDQAADSDKLKSAIQQLVTYAKDLIFQTRSKIQEENSL
NDSFEANETGEMKLPEKIEIPLSKFDVKYFVDGLWTNIVVNRSGQLVPQDSSIPGSLT
LLEQKFASSRSSTSQEPTNRLRMPLETYNTSLAVDTEVISVLEMRIKTVKNILNQFVK
LSNTASSTKSLDATQDRRKILGSSLQALINVSKVIVNIIESIDLTAFYACRASVAESH
SSMTRLFAFLEAKQAIYDALAELEVVRNQNEAGDSNFIAALAEKFEKSKLEEIYFTYE
QTNEQQIKEKVFKLDAVLASVVESAKNLSTELRSIVAVDSRLLDTTGTSSFPRELAAA
AAAAAAVAVSSTAGGNSSNGAMSPRSTYSAKRKESAVDSFSIGSHSIREDGPWFLQLE
HGDEIVYDKKGSSIRGGSQRALVEQLTLHSRLNSEFNVAMLLTFQSFMDATNLFEQLV
ERFLIQPPEGLTSEEFALWAEKKQRPTRLRVVNILKTWLENYWFEDDSEDLTSEARSS
LFESMSKFAEQLKAQKFPGGASLYNIVEKRMNDKEPSFKRMIVTTTTQPPPPILPRNL
KKFKLTEIDPVEMARQLSVREFKLLVVITSHECLLRGCGGLGKSSNSGGRKIGEFIRN
SNCLTNWVAYAILRHSEAKRRASTIRYFIHVAEACRALNNFSSMTAIISALYSSTIHR
MKKTWDYVSSKNTAKLENMNRLMNSSRNFNEYRDLLNLVPPPAVPFFGVYLTDLTFVE
DGNPDYLDTEHKIVNFAKRQKTATIIENIRQFQIVPYNFEELADVQLFLDRGFEEAPP
IEEQYDTSLNFEPREKPGTDKVAKLFEENGIL"
gene complement(<466522..>467007)
/locus_tag="AWJ20_2123"
/db_xref="GeneID:30034000"
mRNA complement(<466522..>467007)
/locus_tag="AWJ20_2123"
/product="hypothetical protein"
/transcript_id="XM_018879052.1"
/db_xref="GeneID:30034000"
CDS complement(466522..467007)
/locus_tag="AWJ20_2123"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737005.1"
/db_xref="GeneID:30034000"
/translation="MPSTLVPCRSATVAADPMTPDRPISRAQAAFFDLKLEAVNMFAI
IVLYPVAVIAAPFHLFYLQSVEWKDRRQNKKLLRSLPITVYNSHPDPVNNSLNLSWGC
SENCNICLDDYVDGKSKLMTLPCGHQFHSTCAVRWIAQQMIAEKKNPAITCPVCKCDI
R"
gene complement(<468365..>468910)
/gene="CCS1"
/locus_tag="AWJ20_2124"
/db_xref="GeneID:30034001"
mRNA complement(<468365..>468910)
/gene="CCS1"
/locus_tag="AWJ20_2124"
/product="Ccs1p"
/transcript_id="XM_018879053.1"
/db_xref="GeneID:30034001"
CDS complement(468365..468910)
/gene="CCS1"
/locus_tag="AWJ20_2124"
/inference="similar to AA sequence:KEGG_Orthology:K04569"
/note="Copper chaperone for superoxide dismutase Sod1p;
involved in oxidative stress protection; Met-X-Cys-X2-Cys
motif within the N-terminal portion is involved in
insertion of copper into Sod1p under conditions of copper
deprivation; required for regulation of yeast copper genes
in response to DNA-damaging agents; protein abundance
increases in response to DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
9295278]; GO_component: GO:0005743 - mitochondrial inner
membrane [Evidence IDA] [PMID 11500508]; GO_component:
GO:0005758 - mitochondrial intermembrane space [Evidence
IEA]; GO_component: GO:0005739 - mitochondrion [Evidence
IEA]; GO_component: GO:0005634 - nucleus [Evidence IDA]
[PMID 18977757]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA,IEA]; GO_function: GO:0016532 -
superoxide dismutase copper chaperone activity [Evidence
IGI,IMP,ISS] [PMID 9295278]; GO_function: GO:0008270 -
zinc ion binding [Evidence IBA]; GO_process: GO:0015680 -
intracellular copper ion transport [Evidence IMP,ISS]
[PMID 9295278]; GO_process: GO:0030001 - metal ion
transport [Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]; GO_process:
GO:0019430 - removal of superoxide radicals [Evidence
IBA]; GO_process: GO:0006801 - superoxide metabolic
process [Evidence IEA]"
/codon_start=1
/product="Ccs1p"
/protein_id="XP_018737006.1"
/db_xref="GeneID:30034001"
/translation="MQNIGKDAIVRGTGEPNSAAVCILESFEDEKNPVKGLARIVGVS
PTRALFDITLNGLPKGKYYPSIRVSGDLSQGAVSTGPQLVELGEIDVSAQLSTSDSFL
ANQPKGFSGHAVFTKDVSISDIIGRGLTVSKSRTANYPDSLVGVIARSAGIWENLKSV
CSCSGKTVWEERSDAISSGIH"
gene <470307..>472679
/gene="IMH1"
/locus_tag="AWJ20_2125"
/db_xref="GeneID:30034002"
mRNA <470307..>472679
/gene="IMH1"
/locus_tag="AWJ20_2125"
/product="Imh1p"
/transcript_id="XM_018879054.1"
/db_xref="GeneID:30034002"
CDS 470307..472679
/gene="IMH1"
/locus_tag="AWJ20_2125"
/note="Protein involved in vesicular transport; mediates
transport between an endosomal compartment and the Golgi,
contains a Golgi-localization (GRIP) domain that interacts
with activated Arl1p-GTP to localize Imh1p to the Golgi;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0005794 - Golgi apparatus [Evidence IDA]
[PMID 15077113]; GO_component: GO:0000139 - Golgi membrane
[Evidence IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 9880327]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0043001 -
Golgi to plasma membrane protein transport [Evidence IMP]
[PMID 16926193]; GO_process: GO:0000042 - protein
targeting to Golgi [Evidence IEA]; GO_process: GO:0015031
- protein transport [Evidence IEA]; GO_process: GO:0006950
- response to stress [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]; GO_process:
GO:0016192 - vesicle-mediated transport [Evidence IMP]
[PMID 9880327]"
/codon_start=1
/product="Imh1p"
/protein_id="XP_018737007.1"
/db_xref="GeneID:30034002"
/translation="MQNAEIRRLTSENSKTIKEATDLKGTLDKAKAEIADLKSNLVKQ
RQLEKDAENFKTEIEALKSQGVSKDELLKQQTEKLAKLDKLEEELKSKDQELTKIKEE
SEKSANAVSADKEKELAILQEKLEKEVSALNEKEEELVKLKSEKEQATVALAKEKDDE
INRLKEIHSKEISELSLKLKEVEESASAASAPPVGSDLADQISTPTSTPSKKKNKKKK
KGGAMATEPPETENTSLPSSDGEISKLQAEIRSKEEHIQELNGMITAIGQDLVVAREE
LANRDNEVTGTKESALQASEELKALKEDMDRQLEEARNKLKEESAALEDTKSKLKEES
ATLEDTKSKLKEESAILEDVRLGLEKAEKLAATRLDELNRSKADYESQISKLKADHGN
STALIDKYKGEISTLTSVVADKEKHISELETKVKSLETSYSKLNAEHNSLKKQQGAEI
ESLQKTNAEARGKLQRELAAVKASKASFEKDHSKLLVEASEAKSKLSAAETEKATAIS
NLQLLQREVDELKQIADESSKRSDSLQEELSDLRKHLQDRTREAQTLTNLRNETEHSY
QTKVKELEAKVRALTEQRDEYESDLLTMQSRKSRELDELRTQNGDLKRQLHMFEEEKN
RYDRDLKDIRKMRDRVQEELTSAKEELASIRKELESSRSQLSAAQTRAVEFEKSASSL
KRAAEESQEKNEVLQRNYRVLTGDLETMRKEIVDLKNKSRDTVHSRQSSIVSLSGQDN
DQVRNERLYTKNFLLNYVDKKEQRSAMLGSLATFLELNDSERQRFATAFR"
gene complement(<472748..>473860)
/gene="CDA2"
/locus_tag="AWJ20_2126"
/db_xref="GeneID:30034003"
mRNA complement(<472748..>473860)
/gene="CDA2"
/locus_tag="AWJ20_2126"
/product="chitin deacetylase CDA2"
/transcript_id="XM_018879055.1"
/db_xref="GeneID:30034003"
CDS complement(472748..473860)
/gene="CDA2"
/locus_tag="AWJ20_2126"
/inference="similar to AA sequence:KEGG_Orthology:K01452"
/note="Chitin deacetylase; together with Cda1p involved in
the biosynthesis ascospore wall component, chitosan;
required for proper rigidity of the ascospore wall;
GO_component: GO:0005631 - chitosan layer of spore wall
[Evidence ISS] [PMID 8940152]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0004099
- chitin deacetylase activity [Evidence IEA]; GO_function:
GO:0004099 - chitin deacetylase activity [Evidence
IDA,ISS] [PMID 8940152]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0016810
- hydrolase activity, acting on carbon-nitrogen (but not
peptide) bonds [Evidence IEA]; GO_process: GO:0030476 -
ascospore wall assembly [Evidence IDA,IMP] [PMID 8940152];
GO_process: GO:0005975 - carbohydrate metabolic process
[Evidence IEA,IEA]; GO_process: GO:0006032 - chitin
catabolic process [Evidence IEA]; GO_process: GO:0000272 -
polysaccharide catabolic process [Evidence IEA];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="chitin deacetylase CDA2"
/protein_id="XP_018737008.1"
/db_xref="GeneID:30034003"
/translation="MKLYATALLVFSTVATAKSNDETKPINPLLLSSPRNIDHSAKYI
NLPISPGKLRENLQNHASGPQTYGTGSKPSHLDRTLADYTVPSRQEIGIGGGISGNNQ
GVKIYAQFKQPFPDWLQSITGLTKWPDENAPYIPLDDIDLSVVPKIPKRSLGDCSNVE
RSHCSFECYRCVAPDDIITCPVMSQTFDDGPSPSTPKLLDQLPIKTTFFTQGINVVRF
PETVRLQHQEGHLLASHTWSHANLPGLSNEDIAAQIQWSIWAINATAGVVPRYFRPPY
GAIDDRVRTITRQFGLVAVFWDKDTFDWRVNDRSKTENEVYQEVRQWQAETPGALILE
HDSTIKTVNVGIEVSRLIHSKQMTVADCVNGPWYND"
gene <474181..>475200
/gene="ARA2"
/locus_tag="AWJ20_2127"
/db_xref="GeneID:30034004"
mRNA <474181..>475200
/gene="ARA2"
/locus_tag="AWJ20_2127"
/product="D-arabinose 1-dehydrogenase (NAD(P)(+)) ARA2"
/transcript_id="XM_018879056.1"
/db_xref="GeneID:30034004"
CDS 474181..475200
/gene="ARA2"
/locus_tag="AWJ20_2127"
/inference="similar to AA sequence:KEGG_Orthology:K00062"
/note="NAD-dependent arabinose dehydrogenase; involved in
biosynthesis of dehydro-D-arabinono-1,4-lactone; similar
to plant L-galactose dehydrogenase; GO_component:
GO:0005575 - cellular_component [Evidence ND];
GO_function: GO:0047816 - D-arabinose 1-dehydrogenase
(NAD) activity [Evidence IEA]; GO_function: GO:0045290 -
D-arabinose 1-dehydrogenase [NAD(P)+] activity [Evidence
IDA,IMP] [PMID 17097644]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_process:
GO:0070485 - dehydro-D-arabinono-1,4-lactone biosynthetic
process [Evidence IMP] [PMID 17097644]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence IEA]"
/codon_start=1
/product="D-arabinose 1-dehydrogenase (NAD(P)(+)) ARA2"
/protein_id="XP_018737009.1"
/db_xref="GeneID:30034004"
/translation="MTQSEVIIKPITTNIPKSIEDLPDIVLGAGVFNYQYTDDPHGLS
AENVLRKAFDLGIRALDTSAYYGPSEEIVGKALKSMASEYPRDTYLICTKAGRVAENN
FDYSAASIRSSVLRSLERLNTTYLDVLYIHDVEFVETKQCLEAIDEAFALKKAGYIKN
VGISGYPLDFLLYLATLAKNTLSHGSLDIILSYSNFCMQNTLLEDYVTKFKTEAGVRV
VLNASPLSMSLLRAVPPHPFHPASLELRKAVSEASVYTSQHGVDISDLAVRFVYTHWT
HGPTVFGLQTVREVETAVQQYWQAKTAAQSKEDEVLLKGVREILGSTLNQTWPSGIEH
PDMTA"
gene complement(<475553..>476314)
/locus_tag="AWJ20_2128"
/db_xref="GeneID:30034005"
mRNA complement(<475553..>476314)
/locus_tag="AWJ20_2128"
/product="hypothetical protein"
/transcript_id="XM_018879057.1"
/db_xref="GeneID:30034005"
CDS complement(475553..476314)
/locus_tag="AWJ20_2128"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737010.1"
/db_xref="GeneID:30034005"
/translation="MKNSTDFQATSPRSQPIFHIENIPASASISSTSLSPTSLAATNS
GPPPIATPGRHSRQSSSSSIYSNSSFQTPAPPTPILGHEPRGLESRGRQASLNNRSSV
SGTSSGGGISTTLAGQGPTKFTGPMSSVEIYDVLEKEQEAIVNKLQREINHLKTERSR
SRSQSTSSSSSMTRNPSIRSIYSVSDAEEQYPGSSAPAGSVATAAATHRRGSRPSFGS
VIDDSSVINSLRKENEVLKKKIAELSIKCKCSQVF"
gene complement(<477351..>477443)
/locus_tag="AWJ20_2129"
/db_xref="GeneID:30034006"
tRNA complement(<477351..>477443)
/locus_tag="AWJ20_2129"
/product="tRNA-Pro"
/db_xref="GeneID:30034006"
gene <477651..>477722
/locus_tag="AWJ20_2130"
/db_xref="GeneID:30034008"
tRNA <477651..>477722
/locus_tag="AWJ20_2130"
/product="tRNA-Glu"
/db_xref="GeneID:30034008"
gene <479962..>481737
/gene="SRF1"
/locus_tag="AWJ20_2131"
/db_xref="GeneID:30034009"
mRNA <479962..>481737
/gene="SRF1"
/locus_tag="AWJ20_2131"
/product="Srf1p"
/transcript_id="XM_018879059.1"
/db_xref="GeneID:30034009"
CDS 479962..481737
/gene="SRF1"
/locus_tag="AWJ20_2131"
/note="Regulator of phospholipase D (Spo14p); interacts
with Spo14p and regulates its catalytic activity; capable
of buffering the toxicity of C16:0 platelet activating
factor, a lipid that accumulates intraneuronally in
Alzheimer's patients; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0016042 -
lipid catabolic process [Evidence IEA]; GO_process:
GO:0006629 - lipid metabolic process [Evidence IEA];
GO_process: GO:0043085 - positive regulation of catalytic
activity [Evidence IMP] [PMID 21347278]"
/codon_start=1
/product="Srf1p"
/protein_id="XP_018737011.1"
/db_xref="GeneID:30034009"
/translation="MASGGGGGDLGLQGIQEANGTSSGSGSGSGSVSWNPHPTTSQGG
GNRSTPITPQKTRSHRSGSAASKVTTPKNPPLSRQETPTKSTASSGSYNVGLVAGTGG
YRLEPRPYPVWAAMSMISKDDKTNESDPPTARSSSGTSKQAYGLGINRGALDRPVFRY
PKLRSETKDKDPVQLVHADDIKQQRNISSSSVNNNEKDEPNDFSQPNAKSTNQQEQAV
SDTIPGTGGPAFFDYSPSAPDPNADTELNEKSKMDVSPGQAFDDRDFSEAQPVPYRRQ
SISYSERDDYKFPARATPSFVIPNNISDNMTRGTSKWQELLAMTARHEFYESQGIIDE
VALNRQGDLDRLWKAHVEEERQLRLEKLRRNRISRLGRLRHFFRKQREPDSTHPGNSG
RDTEPLHISSARYLVSHESRQKYKPRLRKIFLNNPFIPLTLRATIFILSIIALAVSSS
IYKRSHDAKPNRINQQPSTIMAIVVQSTALVYLVYITYDEYSGKPLGLRDARAKMRLI
MLDLLFIIFSAANLSLTFNTLYDRTWVCQDFSFSSSSVTIFLPYISKICDLQKVLASF
LFMVLVMWILTFTVSIFRLVERVAQ"
gene <483005..>484786
/gene="CDC53"
/locus_tag="AWJ20_2132"
/db_xref="GeneID:30034010"
mRNA <483005..>484786
/gene="CDC53"
/locus_tag="AWJ20_2132"
/product="cullin CDC53"
/transcript_id="XM_018879060.1"
/db_xref="GeneID:30034010"
CDS 483005..484786
/gene="CDC53"
/locus_tag="AWJ20_2132"
/inference="similar to AA sequence:KEGG_Orthology:K03347"
/note="Cullin; structural protein of SCF complexes (which
also contain Skp1p, Cdc34p, Hrt1p and an F-box protein)
involved in ubiquitination; SCF promotes the G1-S
transition by targeting G1 cyclins and the Cln-CDK
inhibitor Sic1p for degradation; GO_component: GO:0019005
- SCF ubiquitin ligase complex [Evidence IDA] [PMID
19882662]; GO_component: GO:0019005 - SCF ubiquitin ligase
complex [Evidence IDA,IPI] [PMID 9346238]; GO_component:
GO:0019005 - SCF ubiquitin ligase complex [Evidence
IDA,IPI] [PMID 9346239]; GO_component: GO:0031461 -
cullin-RING ubiquitin ligase complex [Evidence IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0003688 - DNA replication origin binding
[Evidence IPI] [PMID 16421250]; GO_function: GO:0030674 -
protein binding, bridging [Evidence IMP,IPI] [PMID
9499404]; GO_function: GO:0061630 - ubiquitin protein
ligase activity [Evidence IDA] [PMID 9346238];
GO_function: GO:0031625 - ubiquitin protein ligase binding
[Evidence IEA]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence IDA]
[PMID 10385629]; GO_function: GO:0004842 -
ubiquitin-protein transferase activity [Evidence IDA]
[PMID 9346239]; GO_process: GO:0000082 - G1/S transition
of mitotic cell cycle [Evidence IMP] [PMID 8943317];
GO_process: GO:0000086 - G2/M transition of mitotic cell
cycle [Evidence IGI] [PMID 7954792]; GO_process:
GO:0000086 - G2/M transition of mitotic cell cycle
[Evidence IGI] [PMID 9736614]; GO_process: GO:0031146 -
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [Evidence IDA] [PMID 9346238];
GO_process: GO:0031146 - SCF-dependent proteasomal
ubiquitin-dependent protein catabolic process [Evidence
IDA] [PMID 9346239]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0051301 - cell division
[Evidence IEA]; GO_process: GO:0042787 - protein
ubiquitination involved in ubiquitin-dependent protein
catabolic process [Evidence IDA] [PMID 9346238];
GO_process: GO:0042787 - protein ubiquitination involved
in ubiquitin-dependent protein catabolic process [Evidence
IDA] [PMID 9346239]; GO_process: GO:0006511 -
ubiquitin-dependent protein catabolic process [Evidence
IEA]"
/codon_start=1
/product="cullin CDC53"
/protein_id="XP_018737012.1"
/db_xref="GeneID:30034010"
/translation="MIERQRNGEDIPSSSLKQIILSFVSLGLDEQNTKRINLTVYQDF
FETAFLENTRTYYTNESKEFLAHNSVVEYVKKANNRLNEEYQRVKMYLHQYTEERLMS
TCDQVLISDHAAAIQNEFQVLLGDDRQEDFHTMYKLLSRVEGGLVPIQQALQAHVEKQ
GMEAVQKLQSQTTGGPVDPKAYVDSLLVVHMKYSALVSESFENNKDLVRALDNGCRAF
INNNIIAKPTQQLDKDSKTPELLAKYADSLLKKSSKNTGEADIDASLNGIMRIFQYLD
EKDAFEKFYSRLLSKRLVNNSSTSEDAETSMVAKLKEACGYEYTNKLQRMFQDMKTSA
DLQNQFKNTLEKKDVDFTAYVLAQSIWPLPDLKPTFELPKQLVGTFDRFQNFYGTKHS
GRKLMWLWNFCKGEIRANYPKTSKTGYTFQVSVFQIAILLPFNDADTLSFQQLKEITG
LSKEYLANSLHFLLKAKVLLQNPENTEISDESNSFSYNPNFKSKKVRINLNLPLKTEQ
KQDTEDTQKKIQDDRHLFLQAIIVRIMKARKELTHVNLVQETLEQSRRFKPRVPDIKK
TIDTLIEREYLQRVVKANNTTSYQYLA"
gene complement(<484899..>488738)
/gene="SPO71"
/locus_tag="AWJ20_2133"
/db_xref="GeneID:30034011"
mRNA complement(<484899..>488738)
/gene="SPO71"
/locus_tag="AWJ20_2133"
/product="Spo71p"
/transcript_id="XM_018879061.1"
/db_xref="GeneID:30034011"
CDS complement(484899..488738)
/gene="SPO71"
/locus_tag="AWJ20_2133"
/note="Meiosis-specific protein required for spore wall
formation; localizes to prospore membrane (PSM) and is
required for PSM closure during sporulation; mediates PSM
size; interacts with Spo1p and Vps13p and recruits Vps13p
to the PSM during sporulation; mutants exhibit reduction
in PSM PtdIns-phosphate pools; dispensable for both
nuclear divisions during meiosis; contains two PH domains;
GO_component: GO:0005628 - prospore membrane [Evidence
IDA] [PMID 24036347]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0030476 -
ascospore wall assembly [Evidence IMP] [PMID 22611022];
GO_process: GO:0030476 - ascospore wall assembly [Evidence
IEP,IMP] [PMID 9784122]; GO_process: GO:1902657 - protein
localization to prospore membrane [Evidence IDA] [PMID
24036347]; GO_process: GO:0030435 - sporulation resulting
in formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="Spo71p"
/protein_id="XP_018737013.1"
/db_xref="GeneID:30034011"
/translation="MTSSLSATLEEMDPEADHKDPNIIRKSSYTALRLYNEVPSQIDQ
ETRIIFVGPVPMEWLVANKKTYGSWGKMKSLFPKVYSRLWTDQGSQAAANDDVRKITN
AAVAAAANRMAAEQQNGNPVEGVATSSGSIPNTGGPLVAGPEGVIRAIDDLVIAPRIS
VDSTDPMTSFDAVSDDDAWISTDDRPDLVPNHSSDLVPQRYVAADDLVSLNLDSSSIA
EHVLENEDPEPEEEPGENEESSYPHADRTDDLMLSRYLTTEGATSTLRTLPAASDEYP
YNSGSQNSSQQSLSRGSHFYSNTRSSRNENVSHDGDSPLASYGNGPTGIISDDDSRMS
PSPRSTSHLLGDTSRPPSQLDLSSSHSKTFQPALVYKTTPHTNSASEPTPLFTATDSS
VSTMGSMQSYFTARESVVSTGRSDSESSRNNSYFPPQDDYIASNAADSVQSNGSTDDS
STSTVKPNKPHEHSLSGTVPLAAETSTATAVNQIVSPSAKSYLADILERDVAALKKKQ
TSEENKNAQQKQAKKATSVRAPTSKLSNTIQKAKKKPANAGRRVKQKYQKHKHSFMVS
TDKLLKRKRVGEVVMIEKLLVLVKAAPEDRRIPSDFNEMEQIDTRLVQRWREYVVVAR
KSDSSESPIDLQFYKSRQISKIETHDQQHKRKSDKPDFSVGVGSHCEVNLYSSLDKSI
VLWNRTSLGVEPSKKKDSMIYILRARSDASALNWLSFLASIVGICAESLITVKVPDLG
VTIDVEIPFQDIETIRRFEDRSQLVSLKHMMKHSSHPSWMGTYIVEKCLDAMYQVEPI
RKQLDQNWRGIVKAGLVWRRYDRLEWMFDLRGAQMQSSWAMMKTHHELELRPKVPFSL
PIMIQGNPTPLAEPFPIEGFVTRLTTWRGEVRKQKLLNRSYFHTHDNLLFYALPIRAL
PPYVGSDTKDIPLICEIAPYKTDSHGRIEWLNHIKNVEEFDQYDLIAQFEMKRRIASI
SRSDGFVDLIELRDIRPEDDDKVVLVFKSGTILRFQVHDLETRNMWLKQLHDLMIYWK
YRRRERMIRTNAIRQQNLDILHIDEDAEAFVGEAAAKWETDRGVADPLTYNIAGIACS
RSLYMNGILFQKPTKFSTFRKYYVVLTLTDLILYLPSPSSVHWTEGGYCLTERLKSTY
TRVNNYRVQSVSLSECYVYSGPVTSSDLLNRENTFDKENPGRHALPRAYADGWKSAEE
ESLRCFVLWFGKKSYAPTSRKDKKLSFKTVNRLGAGGTSMVFMARSRHDRDLWVTALN
TVMSRLAVDSHEDIVVT"
gene complement(<489511..>490152)
/gene="ATP5"
/locus_tag="AWJ20_2134"
/db_xref="GeneID:30034012"
mRNA complement(<489511..>490152)
/gene="ATP5"
/locus_tag="AWJ20_2134"
/product="F1F0 ATP synthase subunit 5"
/transcript_id="XM_018879062.1"
/db_xref="GeneID:30034012"
CDS complement(489511..490152)
/gene="ATP5"
/locus_tag="AWJ20_2134"
/inference="similar to AA sequence:KEGG_Orthology:K02137"
/note="Subunit 5 of the stator stalk of mitochondrial F1F0
ATP synthase; F1F0 ATP synthase is a large, evolutionarily
conserved enzyme complex required for ATP synthesis;
homologous to bovine subunit OSCP (oligomycin
sensitivity-conferring protein); phosphorylated;
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IEA,IEA];
GO_component: GO:0005753 - mitochondrial
proton-transporting ATP synthase complex [Evidence IDA]
[PMID 10066811]; GO_component: GO:0000274 - mitochondrial
proton-transporting ATP synthase, stator stalk [Evidence
IMP,ISS] [PMID 2146269]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 16823961];
GO_function: GO:0046933 - proton-transporting ATP synthase
activity, rotational mechanism [Evidence IEA];
GO_function: GO:0046933 - proton-transporting ATP synthase
activity, rotational mechanism [Evidence IDA] [PMID
20691145]; GO_function: GO:0046933 - proton-transporting
ATP synthase activity, rotational mechanism [Evidence IMP]
[PMID 7866306]; GO_process: GO:0006754 - ATP biosynthetic
process [Evidence IEA]; GO_process: GO:0015986 - ATP
synthesis coupled proton transport [Evidence IEA];
GO_process: GO:0015986 - ATP synthesis coupled proton
transport [Evidence IMP] [PMID 15254373]; GO_process:
GO:0015986 - ATP synthesis coupled proton transport
[Evidence IDA] [PMID 20691145]; GO_process: GO:0015986 -
ATP synthesis coupled proton transport [Evidence IMP,ISS]
[PMID 2146269]; GO_process: GO:0006811 - ion transport
[Evidence IEA]; GO_process: GO:0015992 - proton transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="F1F0 ATP synthase subunit 5"
/protein_id="XP_018737014.1"
/db_xref="GeneID:30034012"
/translation="MFVRALRQPMALRAIARTYATKASTAPPVQLFGIDGSYASALYT
ASAKDSTIEATEKGLTSLKSLISEDAKLATIIANPALSASDKKTVVEAISQSAGLDKT
VSNFLSVLADNNRLSLLPEVITKFEVLSNAHQGFVDATVTSASPLDDKTLGRLKAAIS
QSEFVGEGKKLRITNDVKSEILGGLVVEIGDRTVDLSVSAKIARLNKLLTDSV"
gene <490818..>492041
/gene="RML2"
/locus_tag="AWJ20_2135"
/db_xref="GeneID:30034013"
mRNA <490818..>492041
/gene="RML2"
/locus_tag="AWJ20_2135"
/product="mitochondrial 54S ribosomal protein RML2"
/transcript_id="XM_018879063.1"
/db_xref="GeneID:30034013"
CDS 490818..492041
/gene="RML2"
/locus_tag="AWJ20_2135"
/note="Mitochondrial ribosomal protein of the large
subunit (L2); has similarity to E. coli L2 ribosomal
protein; mutant allele (fat21) causes inability to utilize
oleate, and induce oleic acid oxidation; may interfere
with activity of the Adr1p transcription factor;
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0015934 - large ribosomal subunit
[Evidence IEA]; GO_component: GO:0005762 - mitochondrial
large ribosomal subunit [Evidence IDA] [PMID 9445368];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA]; GO_component: GO:0030529 - ribonucleoprotein
complex [Evidence IEA]; GO_component: GO:0005840 -
ribosome [Evidence IEA,IEA]; GO_function: GO:0003723 - RNA
binding [Evidence IEA]; GO_function: GO:0003735 -
structural constituent of ribosome [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IDA] [PMID 9445368]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0032543 - mitochondrial translation
[Evidence IMP] [PMID 9079633]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="mitochondrial 54S ribosomal protein RML2"
/protein_id="XP_018737015.1"
/db_xref="GeneID:30034013"
/translation="MSLRLFGLARSTISVASRHRTATGIRFSSTTPSPEENSDPVAVD
QSAGSKRKSTRSEFADSAQSNQLQEELNKLEIVPFGQRSNLTELEEQDNEMKRQLKLF
KTAVKMKSPKPISPSLRWYKWPIYPYLHKGKPVKELTVPRKKHSGRNHHGLITVRHQG
GGAKRRIRLIDFFRLDGGVHEVVRIEYDPNRSSHIALVKNEETSALSYILACVGLRAG
DKVESFRDGIPQRIIDKMGGENDPGILATHIAKKGNCMPLSMIPLGSIIHNIGVSKIG
PGKLCRAAGTYGRLHEKLPEKKKAVIRLQSGEYRYVALDACATLGMVSNPDHQHRSFG
KAGRSRHFGIRPRVRGVAMNKVDHPLGGGRGKSKGNKVPQSPWGTPSKSGYKTRRGKN
INHNKIRDRPRGKNK"
gene complement(<492244..>494664)
/gene="ASG1"
/locus_tag="AWJ20_2136"
/db_xref="GeneID:30034014"
mRNA complement(<492244..>494664)
/gene="ASG1"
/locus_tag="AWJ20_2136"
/product="Asg1p"
/transcript_id="XM_018879064.1"
/db_xref="GeneID:30034014"
CDS complement(492244..494664)
/gene="ASG1"
/locus_tag="AWJ20_2136"
/note="Zinc cluster protein proposed to be a
transcriptional regulator; regulator involved in the
stress response; null mutants have a respiratory
deficiency, calcofluor white sensitivity and slightly
increased cycloheximide resistance; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003677 - DNA binding [Evidence IEA,IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0043565 - sequence-specific DNA
binding [Evidence IDA] [PMID 19111667]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 19158363]; GO_function: GO:0000981 -
sequence-specific DNA binding RNA polymerase II
transcription factor activity [Evidence IEA]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0006357 - regulation of transcription from RNA
polymerase II promoter [Evidence IEA]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA,IEA]; GO_process: GO:0006950 - response to
stress [Evidence IEA]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA,IEA]"
/codon_start=1
/product="Asg1p"
/protein_id="XP_018737016.1"
/db_xref="GeneID:30034014"
/translation="MPCTNCAAFGCECRIPEVRMKKGQRASKKQKTEDGEQAAPGISG
GIAAGLKAQAAFARGKLTPNSKVSARSAITEELREAGDGKSGFTRSILSGKDNWKQFL
DQNLRQPGKMSYLGSTSHVNLIFDNIPDSEAFYFAAAAAAGASNSRVQQMDREEIEIL
KIKGAFLLPAQELCDDLVESYFEKIHPLIPIINRTQFMRQYNDPANPPSLLLRQAVLV
AGSRVCQNPALMDESGSSKLATATFYKRAKALFDANYETDRIAIVQSTLLMAWFLAGP
DYVTDNVYFWTRVSLTIAQSIGLHRSVEKSNVPDIEKRMCKRIWWSLFARDRSTACAM
GRPVMINLDDSDVPMIELEDFDESEPDKPSPYPINREHALYFIAHVKLSEIMGLTIRE
QYSIGAESSRRQNKVPNVNQCDMAMAAWMNNLPPELKYSLKDKRSHDFFKALLHSQYY
TVLCLVHRSNILYRRTPNANGSKIPSYPSWGIAFQAAHMIVRIAENLKSFNELPFCPA
FMVYSLFSAMIILIYQMESPSKSVVESAKRGVESCHGILEILGQVWDTADQICKMARY
LNSDPMLRQKIIFSAKRLAAATSSEEGTENPSAVNSPGPSGVDSGLPTADSSRPGSAP
LKRSYEEEYDPNKIPEVTSPNVSYVRGNQPQPQPQAQRPAHIPGMTPLQPNYQATHLG
QAVPPNMDFPSELFLVTNTPPNPVFFENFQPSQLFPESVRSNSVGQQSSPEMTSDRGA
NMLPDALFASHESFGDVFYENFNSPPEDVGTVPNTLNLGDWYKFLMSTSTASHFPDLR
NVPVKSDV"
gene <499144..>500355
/locus_tag="AWJ20_2137"
/db_xref="GeneID:30034015"
mRNA <499144..>500355
/locus_tag="AWJ20_2137"
/product="putative cysteine synthase"
/transcript_id="XM_018879065.1"
/db_xref="GeneID:30034015"
CDS 499144..500355
/locus_tag="AWJ20_2137"
/inference="similar to AA sequence:KEGG_Orthology:K01738"
/note="Putative cysteine synthase; localized to the
mitochondrial outer membrane; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
16407407]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0004124 - cysteine
synthase activity [Evidence IEA]; GO_function: GO:0003674
- molecular_function [Evidence ND]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0008150 - biological_process [Evidence ND];
GO_process: GO:0008652 - cellular amino acid biosynthetic
process [Evidence IEA]; GO_process: GO:0019344 - cysteine
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0006535 - cysteine biosynthetic process from serine
[Evidence IEA]"
/codon_start=1
/product="putative cysteine synthase"
/protein_id="XP_018737017.1"
/db_xref="GeneID:30034015"
/translation="MAWDQTRLLAVATLSTALVLGSLWYNDVVTVEIKWPVRGKKGIS
KMKTGFVAPKGIEQLIGNTPMVRINSLSDATGCEIYGKMEVQNPGGSAKDRVALAIIE
NAEAQNLITPHSGDMIFEGTSGSTGISLAMLCRAKGYIAHIVVPDDTSQEKVDLLENL
GAVVHKVRPAGIADPKQYVNYAKAAAMEINNNPENKHRALFADQFENEANWKTHYDHT
GPEIYSQISEVLPKGKTLDAFITGAGTGGTISGVSKYLKERLPHVKVVISDPPGSGFY
NKVKYGVMFDLKEKEGTRRRHQVDTVVEGIGLNRITRNFSAGSDYIDDAIRVKDEESV
KMAKYLVDNDGLFVGSSSAVNCVATYKYAKQLGPGHTIVTILCDSGSRHLSKFWKLAK
ESASIKDLSQL"
gene <502421..>504133
/locus_tag="AWJ20_2138"
/db_xref="GeneID:30034016"
mRNA <502421..>504133
/locus_tag="AWJ20_2138"
/product="hypothetical protein"
/transcript_id="XM_018879066.1"
/db_xref="GeneID:30034016"
CDS 502421..504133
/locus_tag="AWJ20_2138"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737018.1"
/db_xref="GeneID:30034016"
/translation="MSRSRFGTGRPFSSPPVNGKTRSTPAPAASGSAVFDFYPMTPDE
QLRTFFIGNLPKGIPDDRVIAFLELISGFQKFVRVTNASGKPLNFGFVRFATPPGTKV
ALDVLPDVEYTDNDGARSKLKLSVESNTLEWLSVKFPHITPEVHQGSSKRDLVTKVNF
VISKWQQASRESKNDENPEDSIDEGDLIEFLNQSIKGSQSQEFASSVSPAMADVLRKE
IKAFREQALRLEKARILETEAYERKKQQELEEKSAKLLQGSLESKATNGDSIITSSEL
GPLMKEFEEYDLSEEELPLVDDEGNNEQRDLKLETKQLERSREDRERLFRKRQERWVN
RELAHLEAVKREKERESNMEERLKTAQENALREYASFSDYDPQSMQKYDYYSDHAHWS
KERNSYRRREIERDNNDIEDERKENEALPDTAIRTTAVNKKISLSLSTAKDRKQVEKP
VQPLKDNEVNASEIIAQLKNDIENKENIFDWDVQWDSLTEDILSNTLRPLVENQILEY
LGIQEEDLVNFVLDHLRSHKPAGELVSELEMTLDEDAPVFVRTLWKQLILEIEMEKRL
KPTN"
gene complement(<504214..>506610)
/gene="SYF1"
/locus_tag="AWJ20_2139"
/db_xref="GeneID:30034017"
mRNA complement(<504214..>506610)
/gene="SYF1"
/locus_tag="AWJ20_2139"
/product="Syf1p"
/transcript_id="XM_018879067.1"
/db_xref="GeneID:30034017"
CDS complement(504214..506610)
/gene="SYF1"
/locus_tag="AWJ20_2139"
/inference="similar to AA sequence:KEGG_Orthology:K12867"
/note="Member of the NineTeen Complex (NTC); that contains
Prp19p and stabilizes U6 snRNA in catalytic forms of the
spliceosome containing U2, U5, and U6 snRNAs; null mutant
has splicing defect and arrests in G2/M; relocalizes to
the cytosol in response to hypoxia; homologs in human and
C. elegans; GO_component: GO:0000974 - Prp19 complex
[Evidence IDA] [PMID 11842115]; GO_component: GO:0071006 -
U2-type catalytic step 1 spliceosome [Evidence IDA] [PMID
11105756]; GO_component: GO:0071006 - U2-type catalytic
step 1 spliceosome [Evidence IDA] [PMID 11842115];
GO_component: GO:0071007 - U2-type catalytic step 2
spliceosome [Evidence IDA] [PMID 11105756]; GO_component:
GO:0071008 - U2-type post-mRNA release spliceosomal
complex [Evidence IDA] [PMID 11105756]; GO_component:
GO:0071004 - U2-type prespliceosome [Evidence IDA] [PMID
11105756]; GO_component: GO:0005829 - cytosol [Evidence
IDA] [PMID 22932476]; GO_component: GO:0005622 -
intracellular [Evidence IEA]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 22932476]; GO_component:
GO:0005681 - spliceosomal complex [Evidence IEA];
GO_function: GO:0000384 - first spliceosomal
transesterification activity [Evidence IC] [PMID
11105756]; GO_function: GO:0000384 - first spliceosomal
transesterification activity [Evidence IC] [PMID
11842115]; GO_function: GO:0000386 - second spliceosomal
transesterification activity [Evidence IC] [PMID
11105756]; GO_process: GO:0006396 - RNA processing
[Evidence IEA]; GO_process: GO:0008380 - RNA splicing
[Evidence IEA]; GO_process: GO:0007049 - cell cycle
[Evidence IEA]; GO_process: GO:0000349 - generation of
catalytic spliceosome for first transesterification step
[Evidence IDA] [PMID 19617314]; GO_process: GO:0006397 -
mRNA processing [Evidence IEA]"
/codon_start=1
/product="Syf1p"
/protein_id="XP_018737019.1"
/db_xref="GeneID:30034017"
/translation="MSTELSLIQEDDILYEQEILKQPGELGPWLRYLEFTADAPISKR
IFVFERACDLFKRSYKLWKMYLDIRVDYVARSHAWILSQQGLNEFSREQLKSEIEKVD
RLYVKALVLLNKMPRLWQDYLEYLLRWKPHSITHIRHTFDNALRALPLSQHHRIWPLY
INFANRHAHISSATAKNIWLRYILFFPDETENCIEQLIDLEYYEQASQLLTKLLNNPN
YVSVKGKSRHQLWEELADILVVPHDNWKPDSFYVERVIKSGIKRYPDQKGKLCVQLAT
YWINNGNFEKARDVFEQGLEDVKTVRDFSQIFDSYCEFEESLIAKLMDEEEQGDLDRL
MESFEQLMDRRPFMINSVLLEQNPNNVVEWEKRAGLWGSSMDQVVQTYEKAIETIVPK
KASGKLYQLWTNYAKLYEKAGDLSTARIIFDKATKVPYKSVNELCDLWIEWAEMELRS
ENLDGAIKIMETATKGPKNSKVDYFDESLSPQERLHKSMKLWSFYVDLVESIGTLEEV
KPIYDRIFELKIGTTLTIVNYANLLEENNYFEEAFKVYERGIEMFSYPISFEIWNIYL
QKAIKRKLGIERLRDLFEQALEDCPSKLCKPLYLLYGKLEEERGLVSNAIKIYERATK
AVDNKDKLETYRYYIARVAENFGLPGTRPIFQTAIDNLNDHDANIIGQEFIKIEEKLG
EIDRVRVLYGFLSQFNDPRTNEEFWSKWDQFEVMHGSEDTYKEMLRIKRSVIAQFNTD
ANYLAAKVSDNNSMSRLDKQTASPIGFVASETLQPSKSEAIDTNDVENPDAIDIEIED
"
gene <507240..>508622
/gene="APE1"
/locus_tag="AWJ20_2140"
/db_xref="GeneID:30034019"
mRNA <507240..>508622
/gene="APE1"
/locus_tag="AWJ20_2140"
/product="Ape1p"
/transcript_id="XM_018879069.1"
/db_xref="GeneID:30034019"
CDS 507240..508622
/gene="APE1"
/locus_tag="AWJ20_2140"
/inference="similar to AA sequence:KEGG_Orthology:K01268"
/note="Vacuolar aminopeptidase yscI; zinc
metalloproteinase that belongs to the peptidase family
M18; often used as a marker protein in studies of
autophagy and cytosol to vacuole targeting (CVT) pathway;
protein increases in abundance and relative distribution
to cytoplasmic foci increases upon DNA replication stress;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
22842922]; GO_component: GO:0000324 - fungal-type vacuole
[Evidence IDA] [PMID 1400574]; GO_component: GO:0005773 -
vacuole [Evidence IEA,IEA]; GO_function: GO:0004177 -
aminopeptidase activity [Evidence IEA,IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0070006 - metalloaminopeptidase
activity [Evidence IDA] [PMID 3882418]; GO_function:
GO:0008237 - metallopeptidase activity [Evidence IEA];
GO_function: GO:0008233 - peptidase activity [Evidence
IEA]; GO_function: GO:0008270 - zinc ion binding [Evidence
IEA]; GO_process: GO:0006508 - proteolysis [Evidence
IEA,IEA]; GO_process: GO:0007039 - vacuolar protein
catabolic process [Evidence ISS] [PMID 2651436];
GO_process: GO:0007039 - vacuolar protein catabolic
process [Evidence ISS] [PMID 2689224]"
/codon_start=1
/product="Ape1p"
/protein_id="XP_018737020.1"
/db_xref="GeneID:30034019"
/translation="MGNNYSEAFIKAIYENPTVYHWCNYTSEFLTARGFQYLPERENW
DGKIKRGGSYYTTRNGSSIVAFKIGSKWKLGDNGVGVIGCHIDALTAKIKPISKKAFK
QGYRQLGVAPYSGGMSSVWWDRDLSIGGRVIVKSQDGSFSTKLAHVEHPIARIPTLAE
HFGKPSEGPFNKETQMTPIIGHETTDEDNATDEEKKSPLYGRHDIKLLRAIAQNVGVS
VSELYQLELELFDSQPGALGGLDREFMFCPRLDDKLCSFGAIQALVETSAPSDAINCV
ALFDNEEIGSLLRQGAKGGLFQHAIQRVFQQLGDFDDDYLRQTFANSFFISSDVTHAV
NPNFSDSYLENHKPKLNTGLVVKLDPNGHTTSDGVSTAFIEEVARRTGNKLQYFHIRN
DGVSGGTIGPMFSSATGIRSVDAGIPQLSMHSIRATTGSRDLELGVKMYSGFFNTWTS
VDADFRKGDI"
gene complement(<508767..>510092)
/gene="THP1"
/locus_tag="AWJ20_2141"
/db_xref="GeneID:30034020"
mRNA complement(<508767..>510092)
/gene="THP1"
/locus_tag="AWJ20_2141"
/product="Thp1p"
/transcript_id="XM_018879070.1"
/db_xref="GeneID:30034020"
CDS complement(508767..510092)
/gene="THP1"
/locus_tag="AWJ20_2141"
/note="Nuclear pore-associated protein; component of
TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in
transcription elongation and mRNA export from the nucleus;
involved in post-transcriptional tethering of active genes
to the nuclear periphery and to non-nascent mRNP; contains
a PAM domain implicated in protein-protein binding;
GO_component: GO:0005635 - nuclear envelope [Evidence
IEA]; GO_component: GO:0005635 - nuclear envelope
[Evidence IDA] [PMID 12206772]; GO_component: GO:0005643 -
nuclear pore [Evidence IDA,IGI] [PMID 12411502];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11139493]; GO_component: GO:0070390 - transcription export
complex 2 [Evidence IDA] [PMID 15311284]; GO_function:
GO:0003723 - RNA binding [Evidence IDA] [PMID 12702719];
GO_function: GO:0003690 - double-stranded DNA binding
[Evidence IDA] [PMID 12702719]; GO_process: GO:0000282 -
cellular bud site selection [Evidence IMP] [PMID
11452010]; GO_process: GO:0031124 - mRNA 3'-end processing
[Evidence IMP] [PMID 18614048]; GO_process: GO:0006406 -
mRNA export from nucleus [Evidence IMP] [PMID 12411502];
GO_process: GO:0051028 - mRNA transport [Evidence IEA];
GO_process: GO:0071033 - nuclear retention of pre-mRNA at
the site of transcription [Evidence IMP] [PMID 18003937];
GO_process: GO:0000973 - posttranscriptional tethering of
RNA polymerase II gene DNA at nuclear periphery [Evidence
IMP] [PMID 18003937]; GO_process: GO:0006368 -
transcription elongation from RNA polymerase II promoter
[Evidence IMP] [PMID 11139493]; GO_process: GO:0006283 -
transcription-coupled nucleotide-excision repair [Evidence
IMP] [PMID 17537816]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Thp1p"
/protein_id="XP_018737021.1"
/db_xref="GeneID:30034020"
/translation="MSVDLWLTRVKEISLIETPVKESSSLLGELFSVDIQNPQVSSLQ
NDILDNGININQKVDAVDIYDGDWPGFVELTKSYLLFCRDVDPSNMVESFYSYGQFLS
DLQTAFSNVRGAILEETVYITTKVVVPQALSLDSQDSQFEMIRTSFLASVLLKIFNSI
RGEKSVGEPLTKKTVIIFVATVLCRLYFKLDQPANCASVFSNIHTANIRFSLYSLAQR
VEYRYWLGRFYLNKNQLSHSYNHLLWSFKNCHSSSPNKRLILLYLLAPAILLGKIPRY
ELLQAYNLDSIYWPLIHSIKRANYQQFMTHLESNEAFFYDHRILVLLQSRSVIILYRM
ILWRLVITSRQLANNDEKAASLVSFPNLQIAVRLSMGSFFPQSAVTDDYQFIESIAVS
LISQGFVKANIYPRMKLIRLKPINALPPVHDVHNANELNLTGRERWMET"
gene <510271..>511653
/locus_tag="AWJ20_2142"
/db_xref="GeneID:30034021"
mRNA <510271..>511653
/locus_tag="AWJ20_2142"
/product="hypothetical protein"
/transcript_id="XM_018879071.1"
/db_xref="GeneID:30034021"
CDS 510271..511653
/locus_tag="AWJ20_2142"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737022.1"
/db_xref="GeneID:30034021"
/translation="MAEINASRRILKPNGNGEPASSTGSIKDAVKLLRQQAAPAKSPT
PPVQAHPEPEKLKVVIRLLPPEIKEKAVLDLLSPWVNINTCNYIYFSEGHVSKKGTKD
PLPSTAYAGFKNAGLLKTFIREFRTLDIQGKLPELGPNVAIEYAPYQKGLRPSKPDPL
AGTLEDDPVYKSFVQRLSDPTVVQVPLIAEEERSKNESKNKGKNKAVAKPSPGSLPKS
ADKNKKRGENSQSVENSREAVKSKTNSLTPTELAAQLTSVPSAGPQSSKKSSSAKAST
QPIGEIANSGEKGSKGSGASSESKSKRGKAKGPKNVLTQNTAAKEETGIPTGPKSQAT
KKKGKAKVVNGDSAPKETSSQNKNASSDNVAPAVPTDPKQKSVRKPAKSKKHSNAENV
SPGPAEPSSSANATGDSTSTTKPKKAHPPRRPAKSKPAADGESPKARPPPRKRDQNSP
KPQPQSQPQP"
gene complement(<512040..>512807)
/locus_tag="AWJ20_2143"
/db_xref="GeneID:30034022"
mRNA complement(<512040..>512807)
/locus_tag="AWJ20_2143"
/product="hypothetical protein"
/transcript_id="XM_018879072.1"
/db_xref="GeneID:30034022"
CDS complement(512040..512807)
/locus_tag="AWJ20_2143"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737023.1"
/db_xref="GeneID:30034022"
/translation="MVAESKVYLITGANRGIGFSLTKFLSERPNTVVIGTARQPEKAQ
ELQALAKEKSNVHVAKYDAAGKDDAKELAKQVSKITDGVDVLIANAAISDPISFTTVK
DAPDHTWLDHHTINVLGPIRLYQAFYDLLKAHETRQIVFVSSLAGSITGYAGLSSSAY
GQSKAALNYTMKEISFEAKEDNFTVISIHPGVVMSDMGEAAKAKVVAEAPQFADFFET
LGITPDESASAQLKVIDALKPEDSGKFLTYLGEETAF"
gene <514387..>515328
/gene="SIT4"
/locus_tag="AWJ20_2144"
/db_xref="GeneID:30034023"
mRNA <514387..>515328
/gene="SIT4"
/locus_tag="AWJ20_2144"
/product="type 2A-related serine/threonine-protein
phosphatase SIT4"
/transcript_id="XM_018879073.1"
/db_xref="GeneID:30034023"
CDS 514387..515328
/gene="SIT4"
/locus_tag="AWJ20_2144"
/inference="similar to AA sequence:KEGG_Orthology:K15427"
/note="Type 2A-related serine-threonine phosphatase;
functions in the G1/S transition of the mitotic cycle;
regulator of COPII coat dephosphorylation; required for ER
to Golgi traffic; interacts with Hrr25p kinase;
cytoplasmic and nuclear protein that modulates functions
mediated by Pkc1p including cell wall and actin
cytoskeleton organization; similar to human PP6;
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 1848673]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA,IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0004721 - phosphoprotein phosphatase
activity [Evidence IEA,IEA]; GO_function: GO:0004722 -
protein serine/threonine phosphatase activity [Evidence
IMP] [PMID 23864707]; GO_process: GO:0006281 - DNA repair
[Evidence IMP] [PMID 15150670]; GO_process: GO:0000082 -
G1/S transition of mitotic cell cycle [Evidence IGI] [PMID
1848673]; GO_process: GO:0031929 - TOR signaling [Evidence
IMP] [PMID 15367655]; GO_process: GO:0030036 - actin
cytoskeleton organization [Evidence IMP] [PMID 12080055];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0051301 - cell division [Evidence IEA];
GO_process: GO:0034599 - cellular response to oxidative
stress [Evidence IMP] [PMID 18357452]; GO_process:
GO:0016311 - dephosphorylation [Evidence IMP] [PMID
15367655]; GO_process: GO:0031505 - fungal-type cell wall
organization [Evidence IMP] [PMID 12080055]; GO_process:
GO:0035556 - intracellular signal transduction [Evidence
IMP] [PMID 12080055]; GO_process: GO:0007067 - mitotic
nuclear division [Evidence IEA]; GO_process: GO:0001302 -
replicative cell aging [Evidence IMP] [PMID 15843932];
GO_process: GO:0002098 - tRNA wobble uridine modification
[Evidence IMP] [PMID 18755837]"
/codon_start=1
/product="type 2A-related serine/threonine-protein
phosphatase SIT4"
/protein_id="XP_018737024.1"
/db_xref="GeneID:30034023"
/translation="MFDSRAARKTVISESELQLLDKQMFPSPYFLQERGLTSLESNIQ
PVQSPVTVCGDIHGQFHDLLELFRVSGGLPDDVNYIFLGDFVDRGYFSLETFTLLMCL
KALYPNRLTLVRGNHESRQITQVYGFYEECLSKYGSTTVWKYCCQVFDFLTLAAIIDG
RILCVHGGLSPEIRMLDQIRVLSRAQEIPHEGGFCDLVWSDPDDVETWAVSPRGAGWI
FGANVAREFNHVNGLNLIARAHQLVMEGFKYHFKDKDVVTVWSAPNYCYRCGNVASVM
KVEDDLVPKFTIFNAVPDDAIKMRLAPQKTQRSEYFL"
gene complement(<517358..>518506)
/gene="LPX1"
/locus_tag="AWJ20_2145"
/db_xref="GeneID:30034024"
mRNA complement(<517358..>518506)
/gene="LPX1"
/locus_tag="AWJ20_2145"
/product="Lpx1p"
/transcript_id="XM_018879074.1"
/db_xref="GeneID:30034024"
CDS complement(517358..518506)
/gene="LPX1"
/locus_tag="AWJ20_2145"
/note="Peroxisomal matrix-localized lipase; required for
normal peroxisome morphology; contains a peroxisomal
targeting signal type 1 (PTS1) and a lipase motif;
peroxisomal import requires the PTS1 receptor, Pex5p and
self-interaction; transcriptionally activated by Yrm1p
along with genes involved in multidrug resistance; oleic
acid inducible; GO_component: GO:0005782 - peroxisomal
matrix [Evidence IEA]; GO_component: GO:0005782 -
peroxisomal matrix [Evidence IDA] [PMID 12135984];
GO_component: GO:0005777 - peroxisome [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016298 - lipase activity [Evidence
IDA] [PMID 18199283]; GO_function: GO:0016298 - lipase
activity [Evidence ISS] [PMID 9200815]; GO_function:
GO:0004806 - triglyceride lipase activity [Evidence IDA]
[PMID 24187129]; GO_process: GO:0007031 - peroxisome
organization [Evidence IEP] [PMID 12135984]; GO_process:
GO:0019433 - triglyceride catabolic process [Evidence IGI]
[PMID 24187129]"
/codon_start=1
/product="Lpx1p"
/protein_id="XP_018737025.1"
/db_xref="GeneID:30034024"
/translation="MVDEHNLTLTIGNGIKISPKITEANYPRGHSRAVLYPDARLKIA
YNVFTLFKNSGNIRPEEALNLVFCHGAQMCKELWNYTIELFFNDPDLGPKLGKVLAID
YVAHGDSYLANTESISFENSWEDASRDINAVVKEEKLIGTTILIGHSMGGMQGLWSAF
YEPGLYDSVVTIDPQGYREEIDPVKSAKILGKKFSKLYDTRALDTFKNYDEYYHYMTK
IGLSAKFHPRCQKDYIDASFVQQEDGSVIMKTPRWSQVSVYISGQTVYRDSLKVLGSL
DCEVLHIFPTQGYKEGSDAIRAALRFATLVDIPNTGHLVPFEQPVETFEAMKGFVLRR
YQRGLAIERQTYARASFSPAQRKQFVKTNFEQIFWGLKQGKSPLHSRL"
gene complement(<519024..>519986)
/locus_tag="AWJ20_2146"
/db_xref="GeneID:30034025"
mRNA complement(<519024..>519986)
/locus_tag="AWJ20_2146"
/product="hypothetical protein"
/transcript_id="XM_018879075.1"
/db_xref="GeneID:30034025"
CDS complement(519024..519986)
/locus_tag="AWJ20_2146"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737026.1"
/db_xref="GeneID:30034025"
/translation="MDRFCSAATGWNTSLTSSDVKRRLPVEGTLWRDEYDGKTPYFNI
SQNSLNPVDDMNALGGYAYLVEATEFLDRIVNFLRRESVDFSSSDDGFSRWFNVFQEL
DSMLVRWKASLPEKWQVSRVHSNGWMDQNLTLAHVTHNASVILLHQNLAYATPDERIQ
ILSSIASETCLTAASEIATIAKKFLYYTENIVAPQFCLCIFIAARALLAHSEFFDSPL
KGEFDSLLSALKEGSKRWPTDQTSQDNLAKQFMDRLEAARDSHTALNVRSAAFEDEAK
LSFSFPQFPDINGILSLSNSLEPTQYLQELDRIVLWDDGVAELE"
gene complement(<521272..>522444)
/locus_tag="AWJ20_2147"
/db_xref="GeneID:30034026"
mRNA complement(<521272..>522444)
/locus_tag="AWJ20_2147"
/product="hypothetical protein"
/transcript_id="XM_018879076.1"
/db_xref="GeneID:30034026"
CDS complement(521272..522444)
/locus_tag="AWJ20_2147"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737027.1"
/db_xref="GeneID:30034026"
/translation="MTYPSSLSLYKEILDTAHLKKIIEVAKLKPTIPTGFPHCTPTGN
TKEYIYEPFEWWTSGFFPGSLWAVKERLTKTDVVGLDENELNALAEDWKTRLEPHQFN
TDTHDIGFLIMPAFWREYQLNRSKKAGEIIVQAAKSLMTRWSDTVQAFRSWTTTTSKR
YNFDNPESDFLVIIDNMMNLDLLYAASIITGDSTYADHATKHADTTMKNQFRDDYSSY
HLIVFDSKTGTKKIGLTCQGYSDSSCWSRGQAWALYGYASVYKYTKNPKYLEFAEKLA
DYFLSRVENGAVFWDFDAPKPCILDISAAMIACSGLLLICELRNDLKYKSKVDTILDF
TMSHAATSSDSVVILDHSTVNNNATANHRIYDSGLVYADYYFLEVGNRLLDMNLLN"
gene complement(<523091..>524659)
/gene="HXT13"
/locus_tag="AWJ20_2148"
/db_xref="GeneID:30034027"
mRNA complement(<523091..>524659)
/gene="HXT13"
/locus_tag="AWJ20_2148"
/product="hexose transporter HXT13"
/transcript_id="XM_018879077.1"
/db_xref="GeneID:30034027"
CDS complement(523091..524659)
/gene="HXT13"
/locus_tag="AWJ20_2148"
/inference="similar to AA sequence:KEGG_Orthology:K08139"
/codon_start=1
/product="hexose transporter HXT13"
/protein_id="XP_018737028.1"
/db_xref="GeneID:30034027"
/translation="MSDTKEVLDLVQEEKTLVDVTPKLERFWVFYPHILKLNTLLVGA
ILAQIVSGFDGSMMNGLQSLVPWQQFFDHPTGGRLGTMSNGVTIGTLIAVPFASLMTD
AIGRRFTIIVGCIIIVIGAILQGAAQNFSMFIGARILLGVGGCWASTAAGPLLAECAY
PTQRPTVSAFLLASWPLGSFVAALVTWGPYRSDLKTSNWSWRIPSLLQGFFPLLQILL
AFLGPESPRYLISKGREDEARAFFTKYHAAGDSEHPVVKFQMAEITAALELEKNDTLS
LKNYLAWFKTKAMRHRLFICIVVPAFQQLSGNAIISYYLTLILNSIGITDSLTQLKIN
IGMTTVSLTADVISAIVVSAYRRRRLFLTGYTSMLLVYIVFTVLSAENQRKNFTDHSL
ASGVVAMIFVYQIAYHIAAPIAIAYIMEVCPYNQRATGSMLYQLSANVVGTFNNYVNP
IAMAAINWRYYIVWICQLVFQISIVYFVFPETAGKDLEDIAEVFGEVGARRAIANSAG
LTEKMEAIEHVESV"
gene <525656..>526498
/gene="GPI12"
/locus_tag="AWJ20_2149"
/db_xref="GeneID:30034028"
mRNA <525656..>526498
/gene="GPI12"
/locus_tag="AWJ20_2149"
/product="Gpi12p"
/transcript_id="XM_018879078.1"
/db_xref="GeneID:30034028"
CDS 525656..526498
/gene="GPI12"
/locus_tag="AWJ20_2149"
/inference="similar to AA sequence:KEGG_Orthology:K03434"
/note="ER membrane protein involved in the second step of
GPI anchor assembly; the second step is the
de-N-acetylation of the
N-acetylglucosaminylphosphatidylinositol intermediate;
functional homolog of human PIG-Lp; GPI stands for
glycosylphosphatidylinositol; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IEA]; GO_component:
GO:0005789 - endoplasmic reticulum membrane [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence ISS] [PMID 10085243]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0000225 - N-acetylglucosaminylphosphatidylinositol
deacetylase activity [Evidence IEA]; GO_function:
GO:0000225 - N-acetylglucosaminylphosphatidylinositol
deacetylase activity [Evidence ISS] [PMID 10085243];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_process: GO:0006506 - GPI anchor biosynthetic
process [Evidence IEA,IEA]; GO_process: GO:0006506 - GPI
anchor biosynthetic process [Evidence ISS] [PMID
10085243]"
/codon_start=1
/product="Gpi12p"
/protein_id="XP_018737029.1"
/db_xref="GeneID:30034028"
/translation="MNLLLPILVFALPRFIWSIITRTIPYGAPFDLTNKNITFLISHP
DDEVMFFGPSLALLTIPEFNNTVSIVSLSSGNSEGLGATRKIELARSAWYFAIPPSRL
FVVENEEDFPDSMEAAWDPKLISDVLGDIPAISNTDAIVTFDSQGVSAHTNHIATGKG
ASLYVQEHSDVGLWKLETVSIYRKYLMFFDAFISYYAVPWVRKVHEQIYDSEWAAKYD
LLKHIPEPTAEPVRASIISRQVQFTQAKSAMIKAHESQMKWYRHGWVIFSRYMIVNDL
IKEN"
gene complement(<526666..>528402)
/locus_tag="AWJ20_2150"
/db_xref="GeneID:30034030"
mRNA complement(<526666..>528402)
/locus_tag="AWJ20_2150"
/product="hypothetical protein"
/transcript_id="XM_018879080.1"
/db_xref="GeneID:30034030"
CDS complement(526666..528402)
/locus_tag="AWJ20_2150"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737030.1"
/db_xref="GeneID:30034030"
/translation="MITRCSRAGPLLVSSAVARSLKSTASNFLRNETKLMSTSQPFAK
DIFIRSEPYREPFDLEGLKSGLVDASMPSDHLETDLDQKFRGDPTSIFKRSEVATAHG
RLGSPIIPSVSHSPNVERLGQFIASKDLNSALHTFQKLIDEDENGLSHFTATQCSQLL
AMIPLGPIKAPQYWPLAQKIASAIKASGHKLQAHDYARLIECAFKAHDYDSIDQIWNE
VQTNLPVSLHSTDLWNKYIMTTCNAYPKFWRQWAGMQVKRNEFRMFKPDETPLAVNDA
VRLLGNMLENNIHPNARTYELVILYLCQKGDLADVRSIIWSIWHITTEPNVLRRSRSK
YRKQLRPSKDSPLAPTISTLKAVIDGFCMNGKVTEALRLMNRLQKEYRIDISGGSRGG
KAIHLWSAVLSWAFITQRPIEGAFTPADTFENVWTIMTEQYGIKPTDEMYRLRGRFFD
SNEKYEDLIADIPEILNSGNPHRLEIARRNLQKAVKGIARQGQIDEAVSIVNEWSQAL
GEFEQVRENLIKYVNTSSTIQRLIAERSPNETNSNTKSSSHDLHDNSIHGETNPFDSN
VNKTNSARLDTV"
gene complement(<528970..>532134)
/gene="YEF3"
/locus_tag="AWJ20_2151"
/db_xref="GeneID:30034031"
mRNA complement(<528970..>532134)
/gene="YEF3"
/locus_tag="AWJ20_2151"
/product="translation elongation factor EF-3"
/transcript_id="XM_018879081.1"
/db_xref="GeneID:30034031"
CDS complement(528970..532134)
/gene="YEF3"
/locus_tag="AWJ20_2151"
/inference="similar to AA sequence:KEGG_Orthology:K03235"
/note="Translational elongation factor EF-3; member of the
ABC superfamily; stimulates EF-1 alpha-dependent binding
of aminoacyl-tRNA by the ribosome; normally expressed in
zinc deficient cells; HEF3 has a paralog, YEF3, that arose
from the whole genome duplication; GO_component:
GO:0022626 - cytosolic ribosome [Evidence IPI] [PMID
9544245]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0016887 - ATPase activity
[Evidence IEA]; GO_function: GO:0016887 - ATPase activity
[Evidence IDA] [PMID 9544245]; GO_function: GO:0003723 -
RNA binding [Evidence IEA]; GO_function: GO:0017111 -
nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_function: GO:0003746 - translation elongation
factor activity [Evidence IEA]; GO_function: GO:0003746 -
translation elongation factor activity [Evidence IGI,ISS]
[PMID 9544245]; GO_process: GO:0006200 - ATP catabolic
process [Evidence IEA]; GO_process: GO:0006412 -
translation [Evidence IEA]; GO_process: GO:0006414 -
translational elongation [Evidence IEA,IEA]; GO_process:
GO:0006414 - translational elongation [Evidence IGI] [PMID
9544245]"
/codon_start=1
/product="translation elongation factor EF-3"
/protein_id="XP_018737031.1"
/db_xref="GeneID:30034031"
/translation="MAAAAEKGESIKVLNELFAKLVVADSVDERKSVAHDVASFINGP
IEEHDLPEKFFADLKGAINNKKDVRAKQNGIEAYGSIAASKELAPSVEPLLVSLTSDV
LEKAGDKNVEVRDAASLAVKELAKNVTPHAVKALLPHLTNSIANSNKWTEKVAALEAL
SILVDSARDQIALRMPELIPVLSEAMWDTKPEVKKAATATITKSTETIDNKDIINFIP
ALIACIAKPTEVPETVHLLGATTFVSEVTTATLSIMVPLLSRGLAERDTAIKRKAAVI
ADNMCKLVDDPQVVAPFLSKLYPALKQNYSNIADPEARDVTLRAINTLKRVGEIGDDD
KIPEISTAGDTGVNLAIIKDLLKDKKVDKRFDVIIEYAAAIAGDLIDERLINEGDWAE
TLRPFLVVFLHDGEARTLIEELRKKSVANIPGGPAFDEEDDEGEDLCNCEFSLAYGAK
ILLNRTQLRLKRARRYGLCGPNGAGKSTLMRAIANGQVEGFPTQDECKTVYVEHDIDG
THADTNVVDYVIATAGEEVGSKETVTKTLVEFGFVNGMTEMPITSLSGGWKMKLALAT
AVLRNADILLLDEPTNHLDTVNVAWLINYLTTCNITSIIVSHDSGFLDKVCQYIIHYE
RYKLRKYKGNLSEFVKKVPAAKSYYELGASDLEFKFPEPGYLEGVKTKQKAIVKVSNM
SFQYPGTSKPQIRDINFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTSGDVYVHEN
CRIAYIKQHAFAHIDNHLDKTPSEYIQWRFQTGEDRETMDRANRQISESDEEAMNKIY
KIEGTPRRVNEVLARRKFKNSYEYECSFFLGENIGMKNERWIAMGSVDNAWIPRSELI
ETHSKLVAEVDMKEALASGQFRPLTRKEIESHCAMLGLEAELVSHSRIRGLSGGQKVK
LVLAACTWQRPHLIVLDEPTNYLDRDSLGALSKAIKEFEGGVVIITHSAEFTKNLTEE
VWAVLDGKMTPSGHNWVTGQGAGPRLDQKEDEEDKFDAFGNKIEAKKKAKKLSAAELR
KKKKERMKKKKELGDAYVSSDDEF"
gene <534748..>534829
/locus_tag="AWJ20_2152"
/db_xref="GeneID:30034032"
tRNA <534748..>534829
/locus_tag="AWJ20_2152"
/product="tRNA-Gln"
/db_xref="GeneID:30034032"
gene complement(<535972..>537381)
/gene="RCK2"
/locus_tag="AWJ20_2153"
/db_xref="GeneID:30034033"
mRNA complement(<535972..>537381)
/gene="RCK2"
/locus_tag="AWJ20_2153"
/product="serine/threonine protein kinase RCK2"
/transcript_id="XM_018879082.1"
/db_xref="GeneID:30034033"
CDS complement(535972..537381)
/gene="RCK2"
/locus_tag="AWJ20_2153"
/codon_start=1
/product="serine/threonine protein kinase RCK2"
/protein_id="XP_018737032.1"
/db_xref="GeneID:30034033"
/translation="MRQLNHKNVVELIDFKETKDHYFIVLELIPGGELFHQIVRLTYF
SEDLARHVIVQVAEAVRYLHEVAGVVHRDIKPENLLFYPIDFIPSKNKTIRKGEDESK
EDEGQFVPGLGAGGIGVIKLADFGLSKVIWDSKTMTPCGTVGYTAPEIVKDERYSKSV
DMWALGCVLYTVLCGFPPFYDESIEVLTEKVAKGQYTFLSPWWDEISAGAKDLVSHLL
TVDPDSRYTIDQFLQHPWITGATSRHAGPAAVAAAATASTGIVGSTGNSLGVIPEIKF
TGSNNSVGTATTDITMINAVPERSQAASASTKEFPTPVVATLKEVFDVSNAVHRMEEE
NARNNRWKGRFQDNLLEEEEEEEEEEDDTAGHGSVTHNEYTMDVEPAYNDPSLDGVEQ
KLQRVSVSKAVPISTTSSKTKASHAVPRSSRKPHNHQHHHGPLATGGLFELHLEGATL
IERRKRAGKQPPITHSLVT"
gene complement(<537421..>538278)
/locus_tag="AWJ20_2154"
/db_xref="GeneID:30034034"
mRNA complement(<537421..>538278)
/locus_tag="AWJ20_2154"
/product="hypothetical protein"
/transcript_id="XM_018879083.1"
/db_xref="GeneID:30034034"
CDS complement(537421..538278)
/locus_tag="AWJ20_2154"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737033.1"
/db_xref="GeneID:30034034"
/translation="MGQQPLLDPAAGVGIAGVPQQVGAPGAGPTGSLGHGYDTRQSVS
EPNLVQTTQQQQQQPQQHTHIQSSQRKEAYDNAVKQIIAEENEERSKIHRYPGLERFD
LIEKMGDGAFSIVYKAYDRTNKTYVAVKIIHKQELSTNQVCFLTRASLLTKHWPPVAA
RDCLNQRTGPVARACAASSPGIPSSVADTVLERPPFGPRCQKSRHFYSGEAWAGGGVP
PAAGAPPQTLVAPLASLESGVGWPANGGWDTWPSPAKTTRAKREEQPGSGAEPQPPEA
DPPLRVSQN"
gene <541873..>543564
/gene="EMP70"
/locus_tag="AWJ20_2155"
/db_xref="GeneID:30034035"
mRNA <541873..>543564
/gene="EMP70"
/locus_tag="AWJ20_2155"
/product="Emp70p"
/transcript_id="XM_018879084.1"
/db_xref="GeneID:30034035"
CDS 541873..543564
/gene="EMP70"
/locus_tag="AWJ20_2155"
/inference="similar to AA sequence:KEGG_Orthology:K17086"
/note="Protein with a role in cellular adhesion and
filamentous growth; also endosome-to-vacuole sorting;
similar to Tmn3p; member of Transmembrane Nine family of
proteins with 9 transmembrane segments; EMP70 has a
paralog, TMN2, that arose from the whole genome
duplication; GO_component: GO:0005768 - endosome [Evidence
IEA]; GO_component: GO:0005768 - endosome [Evidence IDA]
[PMID 8314797]; GO_component: GO:0010008 - endosome
membrane [Evidence IEA]; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
20681974]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006878 - cellular copper
ion homeostasis [Evidence IGI,IMP] [PMID 20681974];
GO_process: GO:0016197 - endosomal transport [Evidence
IMP] [PMID 20526336]; GO_process: GO:0001403 - invasive
growth in response to glucose limitation [Evidence IMP]
[PMID 18178563]; GO_process: GO:0007124 - pseudohyphal
growth [Evidence IGI] [PMID 18178563]; GO_process:
GO:0007034 - vacuolar transport [Evidence IGI] [PMID
20526336]"
/codon_start=1
/product="Emp70p"
/protein_id="XP_018737034.1"
/db_xref="GeneID:30034035"
/translation="MRAAPSLVYNWNIDHLPVTENLKHLESEHDDDDDDDDDDDDDGD
DNAKLNKLFGGRRINRQVWYKGDGIRFGRYDRHKPYIMFYNHFNFEIEYHEHVPGQYR
VVGAAVTPESKNYTTQESAAKCSAPKSPISFPAGRPQNAQSINITYSYSVKWIKSDVR
WASRWDKYLALRGSRIRWKPLITLIVVSTSTILCAVLSEIAFCFVLQEVTKIQQAKSN
PNTITSGLKSLKEVALRSPKNTLLLSVLVGNGCHLFVLALLTIVFAAIGVLSPEKRGS
IATMAIYTYSFTSIIAGYTAAYCFRAYKGEDFVQDEDENEETIIKTDLTLWSTLFLLT
PVLIPGLVFATFLVLNLVIVVRGSDGAVPFPAILKLTGLWFFVSIPLSLLGSLIGKKR
PMNGYKKLVFKSNSPAPEIPKLPFLLKPITRTMLAGLLPFTAVAAELALIANSLWLNQ
VFFMFGFLMATYIILVLGSVVITIGTVYLMLLAGDYRWQWSSFILSGSPAIYVFLSCL
SLLIFRYSYNGLTAILLYVSYSFLLSAAIFFITGSIGFLSTWAFVHYIYSSVRND"
gene <544524..>546212
/gene="EMP70"
/locus_tag="AWJ20_2156"
/db_xref="GeneID:30034036"
mRNA <544524..>546212
/gene="EMP70"
/locus_tag="AWJ20_2156"
/product="Emp70p"
/transcript_id="XM_018879085.1"
/db_xref="GeneID:30034036"
CDS 544524..546212
/gene="EMP70"
/locus_tag="AWJ20_2156"
/inference="similar to AA sequence:KEGG_Orthology:K17086"
/note="Protein with a role in cellular adhesion and
filamentous growth; also endosome-to-vacuole sorting;
similar to Tmn3p; member of Transmembrane Nine family of
proteins with 9 transmembrane segments; EMP70 has a
paralog, TMN2, that arose from the whole genome
duplication; GO_component: GO:0005768 - endosome [Evidence
IEA]; GO_component: GO:0005768 - endosome [Evidence IDA]
[PMID 8314797]; GO_component: GO:0010008 - endosome
membrane [Evidence IEA]; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
20681974]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0006878 - cellular copper
ion homeostasis [Evidence IGI,IMP] [PMID 20681974];
GO_process: GO:0016197 - endosomal transport [Evidence
IMP] [PMID 20526336]; GO_process: GO:0001403 - invasive
growth in response to glucose limitation [Evidence IMP]
[PMID 18178563]; GO_process: GO:0007124 - pseudohyphal
growth [Evidence IGI] [PMID 18178563]; GO_process:
GO:0007034 - vacuolar transport [Evidence IGI] [PMID
20526336]"
/codon_start=1
/product="Emp70p"
/protein_id="XP_018737035.1"
/db_xref="GeneID:30034036"
/translation="MRTANSLAYSWNIDHLPVTENPEHRESEHDDDDDDDVDHDDDDD
NSKLNHFFGERRINRQLWYQGNGVRFGVLDFRGEPHIMFYNHFDFEIEYHEHAPGQYR
VVGAAVTPESKNYTTQESAAKCSAPKSPISFPARRLRGNAQSINITYSYSVKWIKSDV
RWASRWDKYLALRGSRIRWKPLITLIVVSTILCVVLSGIAFYFVQKEVTKIQQAKSNP
NTIMSGLKSLKDVALRSPKNTLLLSVLVGNGSHLFVLALLTIVFAAIGVLSPEKRGSI
ATMAIYTYSFTSIIAGYTAAYCFRAYKGEDFVQDEDENEETIIKTDLTLWSTLFLLTP
VLIPGLVFATFLVLNLVIVVRGSDGAVPFPAILKLTGLWFFVSIPLSLLGSLIGKKRP
MNGYKKLVFKSNSPAPEIPKLPFLLKPITRTMLAGLLPFTAVAAELALIANSLWLNQV
FFMFGFLMATYIILVLGSVVITIGTVYLMLLAGDYRWQWSSFILSGSPAIYVFLSCLS
LLIFRYSYNGLTAILLYVSYSFLLSAAIFFITGSIGFLSTWAFVHYIYSFVRND"
gene complement(<546281..>548509)
/gene="KAR3"
/locus_tag="AWJ20_2157"
/db_xref="GeneID:30034037"
mRNA complement(<546281..>548509)
/gene="KAR3"
/locus_tag="AWJ20_2157"
/product="Kar3p"
/transcript_id="XM_018879086.1"
/db_xref="GeneID:30034037"
CDS complement(546281..548509)
/gene="KAR3"
/locus_tag="AWJ20_2157"
/inference="similar to AA sequence:KEGG_Orthology:K10405"
/note="Minus-end-directed microtubule motor; functions in
mitosis and meiosis, localizes to the spindle pole body
and localization is dependent on functional Cik1p,
required for nuclear fusion during mating; potential
Cdc28p substrate; GO_component: GO:0005737 - cytoplasm
[Evidence IEA]; GO_component: GO:0005881 - cytoplasmic
microtubule [Evidence IDA] [PMID 2138512]; GO_component:
GO:0005856 - cytoskeleton [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0005871 -
kinesin complex [Evidence IEA]; GO_component: GO:0005874 -
microtubule [Evidence IEA]; GO_component: GO:0005874 -
microtubule [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_component: GO:0000922 - spindle pole [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005816 - spindle pole
body [Evidence IEA]; GO_component: GO:0005816 - spindle
pole body [Evidence IDA] [PMID 11729143]; GO_component:
GO:0005816 - spindle pole body [Evidence IDA] [PMID
2138512]; GO_component: GO:0005816 - spindle pole body
[Evidence IDA] [PMID 8106549]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0008017 -
microtubule binding [Evidence IEA]; GO_function:
GO:0008017 - microtubule binding [Evidence IDA] [PMID
22734002]; GO_function: GO:0003777 - microtubule motor
activity [Evidence IEA]; GO_function: GO:0008569 -
minus-end-directed microtubule motor activity [Evidence
IDA] [PMID 8041770]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_function: GO:0046982 - protein
heterodimerization activity [Evidence IPI] [PMID
16107877]; GO_process: GO:0007049 - cell cycle [Evidence
IEA]; GO_process: GO:0051301 - cell division [Evidence
IEA]; GO_process: GO:0000741 - karyogamy [Evidence IEA];
GO_process: GO:0000742 - karyogamy involved in conjugation
with cellular fusion [Evidence IMP] [PMID 2138512];
GO_process: GO:0007126 - meiotic nuclear division
[Evidence IDA,IMP] [PMID 11729143]; GO_process: GO:0008152
- metabolic process [Evidence IEA]; GO_process: GO:0007018
- microtubule-based movement [Evidence IEA]; GO_process:
GO:0007067 - mitotic nuclear division [Evidence IGI] [PMID
9201713]; GO_process: GO:0007064 - mitotic sister
chromatid cohesion [Evidence IGI,IMP] [PMID 14742714];
GO_process: GO:0000743 - nuclear migration involved in
conjugation with cellular fusion [Evidence IMP] [PMID
16380440]; GO_process: GO:0000743 - nuclear migration
involved in conjugation with cellular fusion [Evidence
IMP] [PMID 19386762]; GO_process: GO:0000743 - nuclear
migration involved in conjugation with cellular fusion
[Evidence IMP] [PMID 2138512]"
/codon_start=1
/product="Kar3p"
/protein_id="XP_018737036.1"
/db_xref="GeneID:30034037"
/translation="MELQFLENIPPKTNNANSNATRLQRPTTVRKPTLKSSIPSSRSV
LQERQNIEVTSSKPYSVKRTALPISRNVPIQGRSIKELAPQNHISAIPSIEPRQDQSE
THLQTEIARLRTDEAKLKFERDKLEDKLKAFHDKATEREDTIRREKDQIINSLQNELH
STKDACHLKIVEFQREYNDALSEKIESERQRYQELLSQQQSHYQTQLETHRQQIEHEK
QLEKAASEQKLLHEMELIKHEYERENQKLIFEVENSRSELEKIREESSRQLQEVVGEL
KRENLRLITELQEELKQKEHMYSQEKEAFLTQLQSQREQLNQNMTQISIQARELETTK
MQLTDALANSHSHQTLEKSQQSTIYELQLLLRTAEEHKNIAEKERDIAKEKLFKEETV
RRKLHNQLQELKGNIRVFCRVRPPLQSEQNNQPVAKITFPDKDMESQQLLVCGPASES
AVGSVTTKVHPFAFDKVFPPSSSNEEVFDEVSELIQSALDGYNVCIFAYGQTGSGKTY
TMSGNNGMIPKAVTKIFEASETLKERGWRYVITGEFLEIYNETMRDLLNSSEENKLEI
RHDAKQQKTSIINITSVKLDSPSTVYSVLRQADSNRSVAATAANERSSRSHSVFILRI
TGENVETGEKSEGTLNLIDLAGSERLAHSQATGIRLKETQAINKSLSYLGDVICALGS
EDKSHIPYRNSKLTYLLQYSLSGNSKTLMFVNVSPLEPHVNESLSSLRFATKVNNTKM
KK"
gene <548971..>549747
/gene="RRP15"
/locus_tag="AWJ20_2158"
/db_xref="GeneID:30034038"
mRNA <548971..>549747
/gene="RRP15"
/locus_tag="AWJ20_2158"
/product="Rrp15p"
/transcript_id="XM_018879087.1"
/db_xref="GeneID:30034038"
CDS 548971..549747
/gene="RRP15"
/locus_tag="AWJ20_2158"
/note="Nucleolar protein; constituent of pre-60S ribosomal
particles; required for proper processing of the 27S
pre-rRNA at the A3 and B1 sites to yield mature 5.8S and
25S rRNAs; GO_component: GO:0005730 - nucleolus [Evidence
IEA]; GO_component: GO:0005730 - nucleolus [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 14562095]; GO_component: GO:0030687 -
preribosome, large subunit precursor [Evidence IDA] [PMID
15769876]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0000466 - maturation of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15769876];
GO_process: GO:0000463 - maturation of LSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 15769876]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA]; GO_process:
GO:0042254 - ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Rrp15p"
/protein_id="XP_018737037.1"
/db_xref="GeneID:30034038"
/translation="MAKGSTNVQASKPKGSIKLQKKADVSPPQLKRKREEEAQEEEDE
DASEDNEEEDSDDFEGDSGDYSDDLNDEFSEAEADSSEMEDDQFESEDENVPSKSRLE
AKKEKEQESFAKAMSAILQSKVKAHDRENPILVRSKKTAKDLETSRLDAKARRALAAE
KKQVQDKDRVRNLLEVRPQEDGSEPQTVQQLLEQEKKLRKIAQRGVVRLFNAVLASQT
VVNTASNSGEKILGLTKKEEQMTEMSKDTFLDLIRSGGKK"
gene complement(<550014..>550577)
/gene="YAH1"
/locus_tag="AWJ20_2159"
/db_xref="GeneID:30034039"
mRNA complement(<550014..>550577)
/gene="YAH1"
/locus_tag="AWJ20_2159"
/product="Yah1p"
/transcript_id="XM_018879088.1"
/db_xref="GeneID:30034039"
CDS complement(550014..550577)
/gene="YAH1"
/locus_tag="AWJ20_2159"
/note="Ferredoxin of the mitochondrial matrix; required
for formation of cellular iron-sulfur proteins; involved
in heme A biosynthesis; homologous to human adrenodoxin;
GO_component: GO:0005759 - mitochondrial matrix [Evidence
IEA]; GO_component: GO:0005759 - mitochondrial matrix
[Evidence IDA] [PMID 10375636]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_function: GO:0051537 - 2
iron, 2 sulfur cluster binding [Evidence IEA];
GO_function: GO:0009055 - electron carrier activity
[Evidence IEA]; GO_function: GO:0051536 - iron-sulfur
cluster binding [Evidence IEA,IEA]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0016653 - oxidoreductase activity, acting
on NAD(P)H, heme protein as acceptor [Evidence IGI,IMP]
[PMID 11788607]; GO_process: GO:0006784 - heme a
biosynthetic process [Evidence IGI,IMP] [PMID 11788607];
GO_process: GO:0016226 - iron-sulfur cluster assembly
[Evidence IMP] [PMID 12970193]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]; GO_process:
GO:0006744 - ubiquinone biosynthetic process [Evidence
IMP] [PMID 20534343]; GO_process: GO:0006744 - ubiquinone
biosynthetic process [Evidence IMP] [PMID 21944752]"
/codon_start=1
/product="Yah1p"
/protein_id="XP_018737038.1"
/db_xref="GeneID:30034039"
/translation="MTLRLISKLASPSALRRCALASSKSQAFGTASLLQRSAIRNAVS
GPGGNIYTARSLHTTAIAHHGHVHKPNPGEELHVTFITKEGEQIQLEVAEGDNLLDIA
QAHNMDMEGACGGSCACSTCHVIVDPDYYDKMEEPDDDENDMLDLAFGLTETSRLGCQ
IKMTKELDGVRVALPAMTRNLQARDFQ"
gene complement(<553737..>556373)
/gene="STE6"
/locus_tag="AWJ20_2160"
/db_xref="GeneID:30034041"
mRNA complement(<553737..>556373)
/gene="STE6"
/locus_tag="AWJ20_2160"
/product="ATP-binding cassette alpha-factor transporter
STE6"
/transcript_id="XM_018879090.1"
/db_xref="GeneID:30034041"
CDS complement(553737..556373)
/gene="STE6"
/locus_tag="AWJ20_2160"
/inference="similar to AA sequence:KEGG_Orthology:K05658"
/note="Plasma membrane ATP-binding cassette (ABC)
transporter; required for the export of a-factor,
catalyzes ATP hydrolysis coupled to a-factor transport;
contains 12 transmembrane domains and two ATP binding
domains; expressed only in MATa cells; GO_component:
GO:0005794 - Golgi apparatus [Evidence IDA] [PMID
8045256]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0043332 - mating projection
tip [Evidence IDA] [PMID 12374868]; GO_component:
GO:0043332 - mating projection tip [Evidence IDA] [PMID
7679674]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 7679674]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0016887 -
ATPase activity [Evidence IEA]; GO_function: GO:0042626 -
ATPase activity, coupled to transmembrane movement of
substances [Evidence IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0017111
- nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_function: GO:0015440 - peptide-transporting
ATPase activity [Evidence IDA,IMP] [PMID 11389139];
GO_function: GO:0015440 - peptide-transporting ATPase
activity [Evidence IDA,IMP] [PMID 8626301]; GO_process:
GO:0006200 - ATP catabolic process [Evidence IEA];
GO_process: GO:0008152 - metabolic process [Evidence
IEA,IEA]; GO_process: GO:0000770 - peptide pheromone
export [Evidence IMP] [PMID 2569166]; GO_process:
GO:0019236 - response to pheromone [Evidence IEA];
GO_process: GO:0055085 - transmembrane transport [Evidence
IEA]; GO_process: GO:0006810 - transport [Evidence
IEA,IEA]"
/codon_start=1
/product="ATP-binding cassette alpha-factor transporter
STE6"
/protein_id="XP_018737039.1"
/db_xref="GeneID:30034041"
/translation="MCHILITIKINFKYPTRNEMILDGFNINIKAGQLSFIVGRSGSG
KSTLGHILLKQYERASGAIFIDGLVFDTISPRWLCDNIFVVEQQSILFDVSIDENIKL
AASRATNTESIRNACDQFFVSEFVGELPQGWKTNAGLQGKNLSGGQKQRISLARARLR
NTPIMIFDESFSALDPQMRAKCLEEIRKYRADKTTIIITHELAQINPDDYLYIMESGN
IVEHGLRKDLEADSARLNFLADLSNSSNRASEDLSFSENWFPDLPSKRHSRVDESIAH
RRQSTIDPYNQFISEDEQIDSKVQAWKSKKRISTTKLLFQLINSLPNKIVFGIGIVSA
VLNAITNPLFSFAFSNLLNGIIYQKQMGTSDYSLTRWTVIVIAIAIGDGITTYGRTAL
DIASETWLRHARVRSLRNILDRELLDLNTDTESSLTSLLMNDSESVRLLVSRFTSGVI
SCVILGVFAIVWSMISGWQLSLVGLSFIPCFYFSSAWYKSIVTKWEALYREETTSVIG
LIDEVVAGIKSVKLLALENYFKKEVDKREMTLNAIAFRRAIFIGLGFGVNQIFMYITQ
AIILYYGMTLISDGKYSVQQAMMVFTLLIFSLVTLEQTVGTIPILSNGFETCREILDL
MKRSPSMEKAKAHYDITKGSIKFENVGFWYSSTTSTEDSELEGSGTSPVLLNFNLEVN
SNEVVAITGRSGCGKSTLARLVTKLYSPQRGTISVDGNDISDLSTRSLRKKIAIVSQM
PLSFFEGTIADNLRYGMKDDRTTPERQQMDLMRQACRESGLDEFIMSLEEGYETKMGS
SSSSGSLLSGGQLQRLGIARALLRSPKILILDECTSALDAESIDIIKQMIKYHRTVRD
MTIIIITHQEEMTDGVDRVIKM"
gene <558783..>559064
/gene="OPI3"
/locus_tag="AWJ20_2161"
/db_xref="GeneID:30034042"
mRNA <558783..>559064
/gene="OPI3"
/locus_tag="AWJ20_2161"
/product="bifunctional phosphatidyl-N-methylethanolamine
N-methyltransferase/phosphatidyl-N-dimethylethanolamine
N-methyltransferase"
/transcript_id="XM_018879091.1"
/db_xref="GeneID:30034042"
CDS 558783..559064
/gene="OPI3"
/locus_tag="AWJ20_2161"
/inference="similar to AA sequence:KEGG_Orthology:K00550"
/note="Methylene-fatty-acyl-phospholipid synthase;
catalyzes the last two steps in phosphatidylcholine
biosynthesis; also known as phospholipid
methyltransferase; GO_component: GO:0005783 - endoplasmic
reticulum [Evidence IEA]; GO_component: GO:0005789 -
endoplasmic reticulum membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 2445736];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0008170 -
N-methyltransferase activity [Evidence IEA]; GO_function:
GO:0008168 - methyltransferase activity [Evidence IEA];
GO_function: GO:0080101 -
phosphatidyl-N-dimethylethanolamine N-methyltransferase
activity [Evidence IEA]; GO_function: GO:0080101 -
phosphatidyl-N-dimethylethanolamine N-methyltransferase
activity [Evidence IDA,IGI] [PMID 2445736]; GO_function:
GO:0080101 - phosphatidyl-N-dimethylethanolamine
N-methyltransferase activity [Evidence IMP] [PMID
2684666]; GO_function: GO:0000773 -
phosphatidyl-N-methylethanolamine N-methyltransferase
activity [Evidence IEA]; GO_function: GO:0000773 -
phosphatidyl-N-methylethanolamine N-methyltransferase
activity [Evidence IDA,IGI] [PMID 2445736]; GO_function:
GO:0000773 - phosphatidyl-N-methylethanolamine
N-methyltransferase activity [Evidence IMP] [PMID
2684666]; GO_function: GO:0004608 -
phosphatidylethanolamine N-methyltransferase activity
[Evidence IEA]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_process: GO:0006629 - lipid
metabolic process [Evidence IEA]; GO_process: GO:0032259 -
methylation [Evidence IEA]; GO_process: GO:0006656 -
phosphatidylcholine biosynthetic process [Evidence IEA];
GO_process: GO:0006656 - phosphatidylcholine biosynthetic
process [Evidence IMP] [PMID 23000174]; GO_process:
GO:0006656 - phosphatidylcholine biosynthetic process
[Evidence IDA,IGI] [PMID 2445736]; GO_process: GO:0006656
- phosphatidylcholine biosynthetic process [Evidence IMP]
[PMID 6337128]; GO_process: GO:0008654 - phospholipid
biosynthetic process [Evidence IEA]; GO_process:
GO:0006644 - phospholipid metabolic process [Evidence
IEA]"
/codon_start=1
/product="bifunctional phosphatidyl-N-methylethanolamine
N-methyltransferase/phosphatidyl-N-dimethylethanolamine
N-methyltransferase"
/protein_id="XP_018737040.1"
/db_xref="GeneID:30034042"
/translation="MWALGVTGTYLGDYFGILMDERVTGFPFNVTDNPMYYGSFLSFL
GTGLWFAKPAGIAVSGFVLVMYLIALRFEEPFTAEIYAKRERERAKKAK"
gene complement(<559225..>561030)
/gene="FRE1"
/locus_tag="AWJ20_2162"
/db_xref="GeneID:30034043"
mRNA complement(<559225..>561030)
/gene="FRE1"
/locus_tag="AWJ20_2162"
/product="Fre1p"
/transcript_id="XM_018879092.1"
/db_xref="GeneID:30034043"
CDS complement(559225..561030)
/gene="FRE1"
/locus_tag="AWJ20_2162"
/note="Ferric reductase and cupric reductase; reduces
siderophore-bound iron and oxidized copper prior to uptake
by transporters; expression induced by low copper and iron
levels; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0005886 - plasma membrane [Evidence
IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 17507646]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 8164662];
GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
activity [Evidence IEA]; GO_function: GO:0000293 -
ferric-chelate reductase activity [Evidence IDA] [PMID
8164662]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0016491 - oxidoreductase
activity [Evidence IEA,IEA]; GO_process: GO:0015677 -
copper ion import [Evidence IDA] [PMID 7814363];
GO_process: GO:0006825 - copper ion transport [Evidence
IEA]; GO_process: GO:0006811 - ion transport [Evidence
IEA]; GO_process: GO:0055072 - iron ion homeostasis
[Evidence IEA]; GO_process: GO:0006826 - iron ion
transport [Evidence IDA] [PMID 8164662]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence
IEA,IEA]"
/codon_start=1
/product="Fre1p"
/protein_id="XP_018737041.1"
/db_xref="GeneID:30034043"
/translation="MLASSRSLPPSYSITNQQQHGNEPTEESPFRYDSPFEGPFREEL
IAEIYKGRDLLVYYEYILLGTFLLIVVAHWTNKLLWYIRNKQRNPGLPIYNRNENSTE
ESLISEDTGASSSETDPLLGNRQHSMFKRIYYRTMAFFMFQPEGKGMENYGVTMMLIV
YELMTIFFALYQIRFWIILAFRLGLLATINIPLMYVLGAKHSPLSYLTGWSYEQVNVF
HRLCGAICFFLVIPHLIIFACYFRFDYLLTHLWSVAGIIAGIAFIAIGFSSSLKFRES
FYELFYVVHIVGFLVSIPAVYLHYPTARPYAVAAGLSVLYDRLTRLLLDYRIMKCIIR
PASGETVIMDIPFSPGPKDGTGGFFGRPFAWTTGQHIYITVIGCGTFESHPYTIASTM
KCSKYLRLIIRARDGFSRRLLELAESKHGLDENEPVQQWCILHGPYGVHPPEMPSSAS
PPRNNTKLILVAGGAGVAFTYPLYQEYHLANKLLESQTKLVGQSSPSKFDINFLWVLP
HSSFSEWLVEHGETVVDTTKMDIWATREKGRPDINQLILGYISKANGVSSSEDHCWVA
ACGPDPLLREVRNAVANLRGQGMSNVHYYAERFGW"
gene <561745..>563682
/gene="COG6"
/locus_tag="AWJ20_2163"
/db_xref="GeneID:30034044"
mRNA <561745..>563682
/gene="COG6"
/locus_tag="AWJ20_2163"
/product="Cog6p"
/transcript_id="XM_018879093.1"
/db_xref="GeneID:30034044"
CDS 561745..563682
/gene="COG6"
/locus_tag="AWJ20_2163"
/note="Component of the conserved oligomeric Golgi
complex; a cytosolic tethering complex (Cog1p through
Cog8p) that functions in protein trafficking to mediate
fusion of transport vesicles to Golgi compartments;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IEA];
GO_component: GO:0017119 - Golgi transport complex
[Evidence IEA]; GO_component: GO:0017119 - Golgi transport
complex [Evidence IPI] [PMID 11703943]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0032258 - CVT pathway [Evidence IMP] [PMID 20065092];
GO_process: GO:0006891 - intra-Golgi vesicle-mediated
transport [Evidence IEA]; GO_process: GO:0006891 -
intra-Golgi vesicle-mediated transport [Evidence IGI,IPI]
[PMID 11703943]; GO_process: GO:0015031 - protein
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Cog6p"
/protein_id="XP_018737042.1"
/db_xref="GeneID:30034044"
/translation="MSVSLELLSGKEDVSSGANPDNAISQLITNILSTSYNDPSLRQA
LSVLDTKMEENTAENRRQLRSNIETSVLKSGSTILKDFTKLHQKLSSLGDSIQELNNQ
YNHMEKVVSQATKDTLLIQKQAEELRSSEETISIKKRLLEAYENNFVLTDQELDILNN
GLLASGADEVEQFFQVLQKVNHIHNDCQVLLSADDTSQTGMEIMGQMSAHLDKGYERI
LIAVENEFKKMVSGQYLQISKSLRRYLTILSSERPNLFDSALNGLIDTRQKNLTNEFI
KALTVETSTSKPIDFYAYDILRYVGDIMAWIHSAIVGEQEILETLLLDDKKTLLQKID
KATTSLVKPLRLRIEQVISTEDNLVDIYKVGNVLNFYYSIFIKIFSNESSAIMTAISR
LEEACSRQFSQCLQNKIASQKDGLGAVTRPGSSLLRDLQPPEFFSDALADAKAILSSY
EASINFTSPPSNQFKQTLHDLIEPYLELCNRVSESLPSVDSEIFVINCFDATKSTLSL
FPFVQYKIDQMNNRTDELAEVLEDSQYYKFLSSSGVNSILNADKTLNREKISGNTQLI
QELAASLDNFLPAANMESSVLLHRLSSPRLSSSITLRASQRFVDIFQQIKQCLDELYG
EEAAVLMPRSVSEVKTLLAIE"
gene <564464..>565045
/gene="DET1"
/locus_tag="AWJ20_2164"
/db_xref="GeneID:30034045"
mRNA <564464..>565045
/gene="DET1"
/locus_tag="AWJ20_2164"
/product="acid phosphatase DET1"
/transcript_id="XM_018879094.1"
/db_xref="GeneID:30034045"
CDS 564464..565045
/gene="DET1"
/locus_tag="AWJ20_2164"
/codon_start=1
/product="acid phosphatase DET1"
/protein_id="XP_018737043.1"
/db_xref="GeneID:30034045"
/translation="MREQDFGNFQGDSEEMQRIWTARARYGHFFFRIPNGESAADVYD
RCAGFNESLFRQFNQPHFPEVLVLVSHGIWARVFLMKWYGWSYEKFESLRNVRHCQFL
QMDLCPVKKRYTLMTRLRTWDDTDEEDVREASDGGEIEELEIASNNNISLIDIKKIAA
KEKQIMEEYKSARQDSPGPSYHQNDHEKTIDQH"
gene complement(<565208..>566482)
/gene="DPH1"
/locus_tag="AWJ20_2165"
/db_xref="GeneID:30034046"
mRNA complement(<565208..>566482)
/gene="DPH1"
/locus_tag="AWJ20_2165"
/product="Dph1p"
/transcript_id="XM_018879095.1"
/db_xref="GeneID:30034046"
CDS complement(565208..566482)
/gene="DPH1"
/locus_tag="AWJ20_2165"
/inference="similar to AA sequence:KEGG_Orthology:K07561"
/note="Protein required for synthesis of diphthamide;
required along with Dph2p, Kti11p, Jjj3p, and Dph5p;
diphthamide is a modified histidine residue of translation
elongation factor 2 (Eft1p or Eft2p); may act in a complex
with Dph2p and Kti11p; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0005737
- cytoplasm [Evidence IDA] [PMID 11914276]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0017183 - peptidyl-diphthamide
biosynthetic process from peptidyl-histidine [Evidence
IEA,IEA]; GO_process: GO:0017183 - peptidyl-diphthamide
biosynthetic process from peptidyl-histidine [Evidence
IMP] [PMID 15485916]"
/codon_start=1
/product="Dph1p"
/protein_id="XP_018737044.1"
/db_xref="GeneID:30034046"
/translation="MSEQIGETVPVAPRRKFVGRKKATADAERNGNSNIEDIGAGSLT
QSKRGPVRTVNRIPDDILNDEELNQAIKILPKNYNFEIHKTIWHIRKSNAKRVALQMP
EGLLLYSCIISDILEQFCGVETVIMGDVTYGACCIDDFTALSLNCDFLVHYAHSCLVP
VDVTRMKVLYVFVTIDIDKTHLIATLGKNFAPSSRLALVGTIQFNPTLHAIHDDLLTK
HQIITSTPQAMPLSKGEVLGCTSARLSSKEIDAIVYIGDGRFHLESAMIHNPEIPAYK
YDPYNREFTIETFDHEEMHSIRRAAIKEAKNAKKVGIILGALGRQGNMGTLDLIEKGF
KEKNIETVLVLLSEIFPGKLAQFDDVDCWIQVACPRLSIDWGYAFPRPLLTPYEAMVS
LEKDQPWDKAYPMDYYSKDGYGRGKIPQRIVA"
gene <566854..>568209
/gene="SHQ1"
/locus_tag="AWJ20_2166"
/db_xref="GeneID:30034047"
mRNA <566854..>568209
/gene="SHQ1"
/locus_tag="AWJ20_2166"
/product="Shq1p"
/transcript_id="XM_018879096.1"
/db_xref="GeneID:30034047"
CDS 566854..568209
/gene="SHQ1"
/locus_tag="AWJ20_2166"
/inference="similar to AA sequence:KEGG_Orthology:K14764"
/note="Chaperone protein; required for the assembly of box
H/ACA snoRNPs and thus for pre-rRNA processing; functions
as an RNA mimic; forms a complex with Naf1p and interacts
with H/ACA snoRNP components Nhp2p and Cbf5p; homology
with known Hsp90p cochaperones; relocalizes to the cytosol
in response to hypoxia; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 22932476]; GO_component: GO:0005654 -
nucleoplasm [Evidence IDA] [PMID 12228251]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 22932476];
GO_function: GO:0051082 - unfolded protein binding
[Evidence IDA] [PMID 19426738]; GO_process: GO:0000493 -
box H/ACA snoRNP assembly [Evidence IMP,IPI] [PMID
12228251]"
/codon_start=1
/product="Shq1p"
/protein_id="XP_018737045.1"
/db_xref="GeneID:30034047"
/translation="MITPQFSVDQDDEFIYIKIKAPYIKAQNIEFVVDENVFIFSLHP
YYLRLRFPGKIIDDDDRSSANYDLKTSMVSVKLPKEVKGEHFEDLDMITKLLARKNEE
TTRAAKPTIEEIDTEGGKSGNGDLSVGDSSNEIASATQELDEKNANTKLSSLGEEFDW
EIEQTIPAPLTDSEDQKAVMANKYGFDNGYSGLMSMSVMNGNDINDLSDPDKLSPSER
AKERTELEIEKFDPDYYLSDLFDNPMIEEIISWTETSDDSLTSQESDRLARLSKKTYL
LDSEKQTYIGLVSLLFGSCYDKRINMGDSTVESAWTIGKLSPLISCLDNSFASVQDIM
IGCTRRSLCYPLYRSWALSLAVWNDVYHVLKRGKRAVLKELAKLVLMFDIDLYNVYNQ
ILLEDYAVWIQYSSENVIRSLAHEVHKTTISKESMGFDLLEYEEAAKEALADQDMDQV
D"
gene <568798..>568871
/locus_tag="AWJ20_2167"
/db_xref="GeneID:30034048"
tRNA <568798..>568871
/locus_tag="AWJ20_2167"
/product="tRNA-Ala"
/db_xref="GeneID:30034048"
gene complement(<570067..>570158)
/locus_tag="AWJ20_2168"
/db_xref="GeneID:30034049"
tRNA complement(<570067..>570158)
/locus_tag="AWJ20_2168"
/product="tRNA-Arg"
/db_xref="GeneID:30034049"
gene <571177..>572403
/gene="CDC50"
/locus_tag="AWJ20_2169"
/db_xref="GeneID:30034050"
mRNA <571177..>572403
/gene="CDC50"
/locus_tag="AWJ20_2169"
/product="aminophospholipid translocase regulatory protein
CDC50"
/transcript_id="XM_018879097.1"
/db_xref="GeneID:30034050"
CDS 571177..572403
/gene="CDC50"
/locus_tag="AWJ20_2169"
/note="Endosomal protein that interacts with phospholipid
flippase Drs2p; interaction with Cdc50p is essential for
Drs2p catalytic activity; mutations affect cell polarity
and polarized growth; similar to Lem3p; CDC50 has a
paralog, YNR048W, that arose from the whole genome
duplication; GO_component: GO:0005768 - endosome [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence IEA]; GO_component: GO:0031902 - late
endosome membrane [Evidence IEA]; GO_component: GO:0031902
- late endosome membrane [Evidence IDA] [PMID 12589066];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_component: GO:0005802 - trans-Golgi network [Evidence
IDA] [PMID 15090616]; GO_function: GO:0004012 -
phospholipid-translocating ATPase activity [Evidence IGI]
[PMID 15090616]; GO_process: GO:0051666 - actin cortical
patch localization [Evidence IGI] [PMID 16195350];
GO_process: GO:0006897 - endocytosis [Evidence IGI] [PMID
16195350]; GO_process: GO:0006886 - intracellular protein
transport [Evidence IMP] [PMID 16956384]; GO_process:
GO:0045332 - phospholipid translocation [Evidence IGI]
[PMID 15090616]"
/codon_start=1
/product="aminophospholipid translocase regulatory protein
CDC50"
/protein_id="XP_018737046.1"
/db_xref="GeneID:30034050"
/translation="MDSDTDSIDRPVETKQKSRRPPNTAFRQQRLKAWQPILTPKTVL
PLFFCIAIIFAPIGGLLLYASNQVQEIVIDYSYCSQQAPTDNFANIPSRFVSSQTFSG
GKQPANPPQWKLQVNHDTTPGADNATCFLRFDVPTEMGNPIFMFYRLTNFFQNHRRYV
QSFNEDQLNGKAVSASSLKSPDDCKPLTLAPDGRPYYPCGLIANSWFNDSFSSPVLLN
AAGSSRDNETYVMTDIGIAWSTDRNRFKKSQYKNDEVVPPPNWIKRYPNGYNDDTPIP
DISTWESFQNWMRTAGLPTFSKLALRNDTAPLGTGTYQVAIGLNFEVLEFNGSKSLVL
STRTVIGGRNPFLGIAYLCVAGLCFVLGILFLIKHLIKPRKLGDHSYLSWNQEPAAAG
QATGTDQGNLQQRRHI"
gene <573306..>574433
/gene="ERG6"
/locus_tag="AWJ20_2170"
/db_xref="GeneID:30034052"
mRNA <573306..>574433
/gene="ERG6"
/locus_tag="AWJ20_2170"
/product="sterol 24-C-methyltransferase"
/transcript_id="XM_018879099.1"
/db_xref="GeneID:30034052"
CDS 573306..574433
/gene="ERG6"
/locus_tag="AWJ20_2170"
/inference="similar to AA sequence:KEGG_Orthology:K00559"
/note="Delta(24)-sterol C-methyltransferase; converts
zymosterol to fecosterol in the ergosterol biosynthetic
pathway by methylating position C-24; localized to lipid
particles, the plasma membrane-associated endoplasmic
reticulum, and the mitochondrial outer membrane;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IDA] [PMID 11298754]; GO_component: GO:0005783 -
endoplasmic reticulum [Evidence IDA] [PMID 9450962];
GO_component: GO:0005811 - lipid particle [Evidence IDA]
[PMID 10515935]; GO_component: GO:0005811 - lipid particle
[Evidence IDA] [PMID 24868093]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
16407407]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0008168 - methyltransferase activity [Evidence
IEA,IEA]; GO_function: GO:0003838 - sterol
24-C-methyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0003838 - sterol 24-C-methyltransferase
activity [Evidence IDA] [PMID 18563465]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0006696 - ergosterol biosynthetic process
[Evidence IMP] [PMID 2677674]; GO_process: GO:0006696 -
ergosterol biosynthetic process [Evidence IMP] [PMID
6363386]; GO_process: GO:0006629 - lipid metabolic process
[Evidence IEA]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0032259 - methylation
[Evidence IEA]; GO_process: GO:0006694 - steroid
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0008202 - steroid metabolic process [Evidence IEA];
GO_process: GO:0016126 - sterol biosynthetic process
[Evidence IEA,IEA]"
/codon_start=1
/product="sterol 24-C-methyltransferase"
/protein_id="XP_018737047.1"
/db_xref="GeneID:30034052"
/translation="MSTAKLAPKDFKADAEFAKAMHGSGERGGFTAVLSKNRDAQQAA
VDGYFKHWDNKGPKEETEEDKQARLDDYSSLTKHYYNLVTDFYEYGWGSSFHFSRYYP
GEAFKQATARHEHYLALKMGINENMKVLDVGCGVGGPAREIHRFTGAEVVGLNNNDYQ
ISRGNAYAKKFGLENKLSFVKGDFMQMDFPDNSFDAVYAIEATVHAPDLAGVYGEIYR
VLKPGGTFGVYEWVLTDNYDESNARHREIAYGIEIGDGIPKMFKSEVAEAALKKVGFE
IVHQQDMADVGDKIPWYYPLTGDWKYIQTLGDFFTFCRTSKFGRMFTMEATGLLEKVG
VAPKGSKKVTIALEEAAVHLVNGAKEGLFTPMMLYVCKKAE"
gene <575960..>577597
/gene="HXT6"
/locus_tag="AWJ20_2171"
/db_xref="GeneID:30034053"
mRNA <575960..>577597
/gene="HXT6"
/locus_tag="AWJ20_2171"
/product="hexose transporter HXT6"
/transcript_id="XM_018879100.1"
/db_xref="GeneID:30034053"
CDS 575960..577597
/gene="HXT6"
/locus_tag="AWJ20_2171"
/inference="similar to AA sequence:KEGG_Orthology:K08139"
/codon_start=1
/product="hexose transporter HXT6"
/protein_id="XP_018737048.1"
/db_xref="GeneID:30034053"
/translation="MSEPDNNSLSIKEQPSDDEASSHGPKPVTAKPPLSSYFLVSMLC
MFAAMGGFIFGYDTGTISGYVNMPVFLEKLGSTDANGVHYLSKVRSGLLIGIFSIGAC
IGGIFLSRLGDMYGRKIGVWVGTGVYIVGVLIQVTTYNAWYQFLIGRLIGGMGVGTIA
VLVPMFQSEVAPQEIRGTLVSSYQLMITLGIFIGYIVCYATNTRPDATSYKIPLSLGF
VWSVILIVGLIFLPESPRYLMSKDRNEEAQKIMCWINRRDPNDPLIVETVANVAAAIE
HERNAGKAKWTEVITGKPRVGYRLMVGVMVLALQQLVGANYFFYFGTTIFKGIGMNNS
YLASIIFGAVNFGSTFFGLYFVERFGRRNVLLYGAAGMFIFFVIYASMGAKALYPNGS
DNDANVQVGKAMIFVTCGYIFCFATTWAPCAFVLVSELFPIRTRSKGMSLGLGANWLA
NFLLSFFTPFITGAIHFYYGFVFAGCNLFAIVFVYFFVHETKGLSLEQIDQLFVSGVM
PWRSSGWVAPAIVDPSTYNEEAPKDTHPNAIHDEHIV"
gene <578598..>579479
/gene="AGC1"
/locus_tag="AWJ20_2172"
/db_xref="GeneID:30034054"
mRNA <578598..>579479
/gene="AGC1"
/locus_tag="AWJ20_2172"
/product="Agc1p"
/transcript_id="XM_018879101.1"
/db_xref="GeneID:30034054"
CDS 578598..579479
/gene="AGC1"
/locus_tag="AWJ20_2172"
/note="Mitochondrial amino acid transporter; acts both as
a glutamate uniporter and as an aspartate-glutamate
exchanger; involved in nitrogen metabolism and nitrogen
compound biosynthesis; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005743 - mitochondrial
inner membrane [Evidence IEA,IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence ISS]
[PMID 10930523]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0015183 - L-aspartate transmembrane transporter
activity [Evidence IDA] [PMID 14622413]; GO_function:
GO:0005313 - L-glutamate transmembrane transporter
activity [Evidence IDA] [PMID 14622413]; GO_function:
GO:0015297 - antiporter activity [Evidence IDA] [PMID
14622413]; GO_function: GO:0015292 - uniporter activity
[Evidence IDA] [PMID 14622413]; GO_process: GO:0015813 -
L-glutamate transport [Evidence IDA] [PMID 14622413];
GO_process: GO:0006865 - amino acid transport [Evidence
IEA]; GO_process: GO:0015810 - aspartate transport
[Evidence IDA] [PMID 14622413]; GO_process: GO:0044271 -
cellular nitrogen compound biosynthetic process [Evidence
IMP] [PMID 14622413]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Agc1p"
/protein_id="XP_018737049.1"
/db_xref="GeneID:30034054"
/translation="MALETIAASVLGAVGSKLVFYPLDTIRTLQQTSTNFSYFLPIRS
YFRGLGASLSLSAPAFTVYMVAYRETKRELAPYLGEAALANYIVSGAVSQLTSSFIWT
PMEVLKARMQIHQGPKISTLNLASSIYRMDGIKGFFRGYWMGVAVFLPHSVVWWTTYE
YSKELLSKEDQPLKTIDYGIASASASSSAALASNFLDVIKTRQQVAVSEEIARIRPDD
RQGVFKVARNLIKEVGFFRAFFKGLHIRLLHSLPSSALAMMIVESINPDRLRGTAPAK
FTNDELLPPPALEEIEA"
gene complement(<579704..>581923)
/gene="FAA2"
/locus_tag="AWJ20_2173"
/db_xref="GeneID:30034055"
mRNA complement(<579704..>581923)
/gene="FAA2"
/locus_tag="AWJ20_2173"
/product="medium-chain fatty acid-CoA ligase FAA2"
/transcript_id="XM_018879102.1"
/db_xref="GeneID:30034055"
CDS complement(579704..581923)
/gene="FAA2"
/locus_tag="AWJ20_2173"
/inference="similar to AA sequence:KEGG_Orthology:K01897"
/note="Medium chain fatty acyl-CoA synthetase; activates
imported fatty acids; accepts a wide range of fatty acid
chain lengths with a preference for medium chains,
C9:0-C13:0; localized to the peroxisome; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005777 -
peroxisome [Evidence IDA] [PMID 8670886]; GO_function:
GO:0005524 - ATP binding [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
activity [Evidence IEA]; GO_function: GO:0004467 -
long-chain fatty acid-CoA ligase activity [Evidence IDA]
[PMID 8206942]; GO_function: GO:0031956 - medium-chain
fatty acid-CoA ligase activity [Evidence IDA] [PMID
8206942]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA]; GO_function: GO:0031957 - very long-chain
fatty acid-CoA ligase activity [Evidence IGI] [PMID
9988704]; GO_process: GO:0006631 - fatty acid metabolic
process [Evidence IEA]; GO_process: GO:0006629 - lipid
metabolic process [Evidence IEA]; GO_process: GO:0001676 -
long-chain fatty acid metabolic process [Evidence IGI,IMP]
[PMID 7962057]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]"
/codon_start=1
/product="medium-chain fatty acid-CoA ligase FAA2"
/protein_id="XP_018737050.1"
/db_xref="GeneID:30034055"
/translation="MASQNENGFTEETLRRIWKYNFVLPDGINENTSPFDTTLSSHAA
QGKPQALPVPGTKQPGYSEIYRNQCSPDKVISGYHPSVKTFYDAFLTVATTYPKRDCI
ADREYDQKLKKWGNYKWLSFEETAKRRTKIAAGLVHAVRKATGFDPADKKYIVSVYAP
NCVNWILTDLACQTQSLPTVCLYDTLGPHTSEYILNLCESPIVVCSLANVPRILSLKH
KLPHVKVIVSTNSFESVPGQFEPAGQSKKELLNVWANDQGVALYSLNEIEALGADFPI
ADRPPKPEDIYAINFTSGTTGNPKGAILTHANVVAAIAMGRHCLGKNFKPDQVATVYS
FLPLAHIYERIAMCIIMSLGHRQAYPHGPVTEIFEDIQILKPTNVNMVPRVLNRVAAA
LRASTIEAPGLAGAISRKAHEAKLNHLYATGSPNHPIWDFLWSRKIRKKLGFDNLNSL
VSGSAPLSKATVEFLKVALAAELLQGYGLTESMSGISVSQPEEPLAGSCGAVAPTTEL
RFRDVPELGYTANDKPFPRGEIMLRGPQMFTGYYKNNEKTLEAFDEDGWFHTGDVGTV
DKLGRIFIIDRVKNFFKLSQGEYVAAEKIENTYLANSSLITQIFVHGDSTESYLVAIA
GINPDSYATLANKVLHKRIRPTDLAALAATYDVPEVKAAFIKALNGSTGNLLQGFEKI
KNVLLRFEPLTPENDTLTPTLKIKRPAAVKLFKAEIDAMYKEGPLSDSGALNSTPKL"
gene complement(<582911..>583297)
/gene="NHP6B"
/locus_tag="AWJ20_2174"
/db_xref="GeneID:30034056"
mRNA complement(<582911..>583297)
/gene="NHP6B"
/locus_tag="AWJ20_2174"
/product="Nhp6bp"
/transcript_id="XM_018879103.1"
/db_xref="GeneID:30034056"
CDS complement(582911..583297)
/gene="NHP6B"
/locus_tag="AWJ20_2174"
/note="High-mobility group (HMG) protein; binds to and
remodels nucleosomes; involved in recruiting FACT and
other chromatin remodelling complexes to the chromosomes;
functionally redundant with Nhp6Ap; required for
transcriptional initiation fidelity of some tRNA genes;
homologous to mammalian HMGB1 and HMGB2; NHP6B has a
paralog, NHP6A, that arose from the whole genome
duplication; GO_component: GO:0005694 - chromosome
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 3169249]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0008301 - DNA
binding, bending [Evidence IDA] [PMID 7721780];
GO_function: GO:0031491 - nucleosome binding [Evidence
ISS] [PMID 11432837]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IDA] [PMID
19158363]; GO_process: GO:0006281 - DNA repair [Evidence
IEA]; GO_process: GO:0070898 - RNA polymerase III
transcriptional preinitiation complex assembly [Evidence
IGI] [PMID 11287614]; GO_process: GO:0070898 - RNA
polymerase III transcriptional preinitiation complex
assembly [Evidence IDA] [PMID 17178828]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEA]; GO_process: GO:0006338 - chromatin
remodeling [Evidence IGI] [PMID 14739928]; GO_process:
GO:0001195 - maintenance of transcriptional fidelity
during DNA-templated transcription elongation from RNA
polymerase III promoter [Evidence IDA] [PMID 16407207];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IGI] [PMID 8946917]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Nhp6bp"
/protein_id="XP_018737051.1"
/db_xref="GeneID:30034056"
/translation="MFGQLTRGAMALPAVRPLALATSAVSMRTPGLIANAHSKASGDD
KVKRKKKDPNAPKRASSAYMFFVKANRDEVLSENPGITFGETGRALGKKWNNLSSAEK
APYEEKANEDKIRYQEAKAAYDASKA"
gene complement(<583770..>584735)
/gene="ENV9"
/locus_tag="AWJ20_2175"
/db_xref="GeneID:30034057"
mRNA complement(<583770..>584735)
/gene="ENV9"
/locus_tag="AWJ20_2175"
/product="Env9p"
/transcript_id="XM_018879104.1"
/db_xref="GeneID:30034057"
CDS complement(583770..584735)
/gene="ENV9"
/locus_tag="AWJ20_2175"
/note="Protein proposed to be involved in vacuolar
functions; mutant shows defect in CPY processing and
defects in vacuolar morphology; has similarity to
oxidoreductases, found in lipid particles; required for
replication of Brome mosaic virus in S. cerevisiae, a
model system for studying replication of positive-strand
RNA viruses in their natural hosts; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0005811 - lipid particle [Evidence
IEA,IEA]; GO_component: GO:0005811 - lipid particle
[Evidence IDA] [PMID 14562095]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence ISS] [PMID
8972580]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]; GO_process:
GO:0006624 - vacuolar protein processing [Evidence IMP]
[PMID 21912603]; GO_process: GO:0007033 - vacuole
organization [Evidence IMP] [PMID 21912603]"
/codon_start=1
/product="Env9p"
/protein_id="XP_018737052.1"
/db_xref="GeneID:30034057"
/translation="MTFDSKTSSEEVAQAFSSEIKGKVFLVTGATWGGIGADNARVLS
GHGAKLVIVTGRSQSKLDETIGNIKKENPNANIRGLILDLSSFKSIRKAAEEVNNYSE
NIDVLINNAGVMACPYSKTEDGFELQFGTNHLGHFLFTNLILKKILSSPHPRVVNLSS
MGHGLAPIIFGDIGFSDGATYERFHAYGQSKTANVLFSRELSRRYKSQGLVSFSVHPG
AIMDTNLGRHMNFDGDINNIGIPKDYWGTDDFYHDMANVRMKNVTQGGATTLVAALDP
AIASQSGAYLDDCQIHEDFCRNHAKQPNDAFKLWNLSEELVGQKF"
gene complement(<585581..>587191)
/gene="DIP5"
/locus_tag="AWJ20_2176"
/db_xref="GeneID:30034058"
mRNA complement(<585581..>587191)
/gene="DIP5"
/locus_tag="AWJ20_2176"
/product="Dip5p"
/transcript_id="XM_018879105.1"
/db_xref="GeneID:30034058"
CDS complement(585581..587191)
/gene="DIP5"
/locus_tag="AWJ20_2176"
/inference="similar to AA sequence:KEGG_Orthology:K16261"
/note="Dicarboxylic amino acid permease; mediates
high-affinity and high-capacity transport of L-glutamate
and L-aspartate; also a transporter for Gln, Asn, Ser,
Ala, and Gly; relocalizes from plasma membrane to vacuole
upon DNA replication stress; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
22842922]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_component: GO:0005886 - plasma membrane
[Evidence ISS] [PMID 10654085]; GO_component: GO:0005886 -
plasma membrane [Evidence IDA] [PMID 22842922];
GO_function: GO:0015171 - amino acid transmembrane
transporter activity [Evidence IEA]; GO_function:
GO:0015171 - amino acid transmembrane transporter activity
[Evidence IDA] [PMID 10654085]; GO_process: GO:0003333 -
amino acid transmembrane transport [Evidence IEA];
GO_process: GO:0006865 - amino acid transport [Evidence
IEA,IEA]; GO_process: GO:0006865 - amino acid transport
[Evidence IDA] [PMID 10654085]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA,IEA]"
/codon_start=1
/product="Dip5p"
/protein_id="XP_018737053.1"
/db_xref="GeneID:30034058"
/translation="MSEDYGNEKNFKVTIYDDSASDEITVGVFKEDKTRLTKGLKQRH
IQMIALVGVIGTGLFLYSGGALQSAGPLGMLLAYLLVGTIVGFNQIAVAEVSAFMPTT
GSTIRQLEHFIDPAFGFAFGYLQFWWAVIPSEFVAASVVVSYWSDLNPAIWLTIFIVL
AILANSWSIRLYGEIEFFFACLKILLILGLILTGLIIDLGGVKGQPRLGFHYWKHPGV
WAEFMKTGAAGRTIGFFSVMNGAVYSFGGLQTVALLAGETQNPRRNIPKAAKRILARV
LTFYMLALFIVSLIVPYDNPDISNGSGNASGSPFVVAMKLAGIKVLPSIVNAMVLCSA
WSAASGSLVQGSRILFSLAANNQAPKVFLRTNKRGLPWVGLIVTGLFLPLSYMSLSSS
SSKVFGYLVNVTSSIILIQWILIASIHFKMNKALDIQGHSRHELPYHFKGGRYLGIIS
GIGSLLLLLIGGFTIFISWSTSQFVSAYVSIPLFLAFSLFWKLYKKTKWVEADKVDLK
SLFLEIENNPEPEEVPLRGWHRLEFLWA"
gene <588233..>588670
/gene="RPS23B"
/locus_tag="AWJ20_2177"
/db_xref="GeneID:30034059"
mRNA <588233..>588670
/gene="RPS23B"
/locus_tag="AWJ20_2177"
/product="ribosomal 40S subunit protein S23B"
/transcript_id="XM_018879106.1"
/db_xref="GeneID:30034059"
CDS 588233..588670
/gene="RPS23B"
/locus_tag="AWJ20_2177"
/inference="similar to AA sequence:KEGG_Orthology:K02973"
/note="Ribosomal protein 28 (rp28) of the small (40S)
ribosomal subunit; required for translational accuracy;
homologous to mammalian ribosomal protein S23 and
bacterial S12; RPS23B has a paralog, RPS23A, that arose
from the whole genome duplication; deletion of both RPS23A
and RPS23B is lethal; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0022627 - cytosolic
small ribosomal subunit [Evidence IDA] [PMID 6814480];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IEA,IEA]; GO_component: GO:0015935 - small
ribosomal subunit [Evidence IEA]; GO_function: GO:0003735
- structural constituent of ribosome [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IDA] [PMID 6814480]; GO_process:
GO:0000462 - maturation of SSU-rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IGI]
[PMID 16246728]; GO_process: GO:0006450 - regulation of
translational fidelity [Evidence IGI,IMP] [PMID 8415737];
GO_process: GO:0006450 - regulation of translational
fidelity [Evidence IGI,IMP] [PMID 8950190]; GO_process:
GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="ribosomal 40S subunit protein S23B"
/protein_id="XP_018737054.1"
/db_xref="GeneID:30034059"
/translation="MGKGKPRGLNSARKLRTTRRDNRWADLSYKKRLLGTAFKSSPFG
GSSHAKGIVLEKIGVEAKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEV
LLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS"
gene complement(<589164..>590843)
/gene="RRP9"
/locus_tag="AWJ20_2178"
/db_xref="GeneID:30034060"
mRNA complement(<589164..>590843)
/gene="RRP9"
/locus_tag="AWJ20_2178"
/product="Rrp9p"
/transcript_id="XM_018879107.1"
/db_xref="GeneID:30034060"
CDS complement(589164..590843)
/gene="RRP9"
/locus_tag="AWJ20_2178"
/inference="similar to AA sequence:KEGG_Orthology:K14793"
/note="Protein involved in pre-rRNA processing; associated
with U3 snRNP; component of small ribosomal subunit (SSU)
processosome; ortholog of the human U3-55k protein;
GO_component: GO:0030686 - 90S preribosome [Evidence IDA]
[PMID 12150911]; GO_component: GO:0031428 - box C/D snoRNP
complex [Evidence IDA] [PMID 11081632]; GO_component:
GO:0005730 - nucleolus [Evidence IEA]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 12150911];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0032040 - small-subunit
processome [Evidence IDA] [PMID 12068309]; GO_function:
GO:0030515 - snoRNA binding [Evidence IPI] [PMID
11105764]; GO_process: GO:0000154 - rRNA modification
[Evidence TAS] [PMID 10690410]; GO_process: GO:0006364 -
rRNA processing [Evidence IEA]; GO_process: GO:0006364 -
rRNA processing [Evidence TAS] [PMID 10690410];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA]"
/codon_start=1
/product="Rrp9p"
/protein_id="XP_018737055.1"
/db_xref="GeneID:30034060"
/translation="MVDSFFVNPTKRRKTASSSGGSGSNKRAATSNGIRKNGPSRVNG
RSNNKSQNGRASDDENNDDEGLESDDLDSDVSMEDDDNVNSDDSEDEFGNETEADKRR
RLAKQYLENIQTELTEAGDDAFDAKDLDREIISRRLKEDVAETVGKVYRFIGDSFDFK
SVSPKNGTAQLIQAKAYGITSVATNYPFAYTTSKDLTLTKWDISNAKKPRVVRFVKGN
HRVKTPEERETFKGHFDEILCVAASSDGKYVVTGGRDRRLVVWSAESLAPIKVFETRD
RKGVVMGLVFRRNTNELYASCADLKVRTFNLDQLAQVEILFGHQDEVADIAALGQERC
VSVGSRDRTAIIWKIAEETRLTFRGADTVSGSTSNKSGSKSKVNDSGYDAYAPTKKIL
EGSIDCCSMIDDQLFVTGSDNGNVSLWSVNKKKPLFVLREAHGRDESLLPDQVSAETA
AGVSVPPPPALPRYITSIYAIPFSDTFFTGSWDGQIRAWKLSADLRTFEPLATVSGAS
GIVNRISVVETGSKGKETFTIVGAVAKELRLGRWLKVKGKNGLFTATIARK"
gene complement(<593497..>595758)
/locus_tag="AWJ20_2179"
/db_xref="GeneID:30034061"
mRNA complement(<593497..>595758)
/locus_tag="AWJ20_2179"
/product="hypothetical protein"
/transcript_id="XM_018879108.1"
/db_xref="GeneID:30034061"
CDS complement(593497..595758)
/locus_tag="AWJ20_2179"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737056.1"
/db_xref="GeneID:30034061"
/translation="MQAARNLEKRFSLDSISSRIIARHWDQEQLDQLDQQQQNEPPFD
TSQPTQSHLKYQRPRPRQRQRRTQDLGQSLRDQNQDPSHILTHILDQNSPESSQQQEQ
QETQNSLSRNYNCHCTNSPCDCFVLLTKKIDMLQKDLNVAVEVGQALLKTHEDYVAES
QAEAAQMEESINSMTLNLQSVMRENDKLLVNNSELISQLHENSKALQESDSKIEKLSH
ELNSSKDRIIRLNTFMLQAKELEAQISSLEMIRQGLQDDLQQTEREKQLAELKWRKAE
RLLEKLTDQYEKLEEESVQSQKPTASRLAFLNDQSIKKTISDLQKNVLGANERANIPA
TSTPVKYKHETWSANTSSQQPQFPMLISPPSSGKRGRSHLRNSSKSTLNGQPLGPLYE
DESFREFFGDESADSSFPYYSSNQHDIKREYVLSSPLHSRQNSCLANEYRTASDNYGS
ASGKDSPLDSSSHEPIRNTVEFNNGLPTPNDTLEHEIDLPETQTSSDDINHEERMDES
INQDSDRSVTSNTESVESTPAGPSSPTDSIKSSASTATSIPSSALTPISPRYEPMLRR
AASHESIFSVFVETHLHNGHSRNSSTTTAAIDLSPALGPFSHKGLPSFYSQRTGVALA
EISTEMSLAPPSKLLNPEYSNSQRSRQLLFAAISNNTKQAVKIPARRNSIESSTTSDL
SGSSDQDRPSSSWASLFSRLRNPTSTTNTTPTSTPRSPADTLIAKILADPIQTHVLAS
PSDVSEAMNSTIL"
gene <598692..>602078
/gene="ALK2"
/locus_tag="AWJ20_2180"
/db_xref="GeneID:30034063"
mRNA <598692..>602078
/gene="ALK2"
/locus_tag="AWJ20_2180"
/product="protein kinase ALK2"
/transcript_id="XM_018879110.1"
/db_xref="GeneID:30034063"
CDS 598692..602078
/gene="ALK2"
/locus_tag="AWJ20_2180"
/inference="similar to AA sequence:KEGG_Orthology:K16315"
/codon_start=1
/product="protein kinase ALK2"
/protein_id="XP_018737057.1"
/db_xref="GeneID:30034063"
/translation="MDTAVETKQRKKLFGHNRSGSLFQSFSSTTSKTYGKKKANPTLA
KAQAGFQQWSQIRAADASNANIINNSQASGPGPVLASGDDLEDDDADIQNDSRKEVHA
TTAPIGLGIDSLIPLEIDTSNKENISWEAETTEVLRKIRPTSKSSLRGINVNGVNPGG
AGSVTGSATTSATASINGEIAEDSPKQNRLSRLVRTISGLSTTSTSSKDTIDGTKLNR
NSTATVSRVSHRSSLSTIHRFSIGSTSSSTDATPVKPQSNRYSALLDVGNLRRSSVAN
NLSQILNNSDLDTDEEDEEDDMDEGTGKRAIPGIVVPFENDLEGSKKNRESWNDNYSK
QRTRPSSEYRDVSHDVGLLNIPSNYPPMASSNRMSYMSLRKPAPPALTISSTSPQSST
VTSPALSHYSNVSIPSISSGSGHSPLLSYKKETGSIKHKRVTKDSISGPTSLVNHQEF
FAPRPSFESDFDSYSNGRSASQPANTISGGPSSISTSVSSTGTSNSSASRTSRYGSSF
LTSITPHSSLLSLRSTKAKERSGTSTSDFVPNKSPTSAASISSSINLPASSSNTTAKS
STKSKHAHKLSLVNGFTKKPSIQDMKRYISFSPSSSSLFRNASNSPTNGSATMTKSPS
ILGDSFDKTMISLPIPVDGSREKLRNKLRASTSLLSLARSDTSGSLTVGIPIAEYEQS
QLQTLLSLCSCAEIEDFSDYIDHGYDNLEKLAEASFSEVFIQRSSNSSRILKIIPFGN
EELDQLPVQDIIQELTIARMLMPLDGYVDVLDSTIVRGTYPQRLLELWDDYDQQKRSE
NYRPDFFKHDQMYCIVVMANAGTDLEHFDLSSWQDAESIFWQVVQSLALAEEKFEFEH
RDLHWGNVVIEEVSKGVSQTTDLLNNLSLEDEQRNTSKRTNPKDKYGFRYDEDEADGE
IDDDLYEEKELKVTLIDYTLSRAMTDDGTVVFTRLDHPDFFRGKGDYQFDIYRFMRTF
IGGASASYLSPNPANHATQLASTSPGLGHPSPDLAKLNQQTNWSLFCPRTNILWLHYL
VDKLLNAKSLPRVSTTRSGRLSFGGGSNGSLRDAYLKGNNVSHNDEARACAALEIIHK
TLDPRKKRFGGKKHNSPMSFQDFSSARDVLRWGVRNKYVS"
gene <605132..>606229
/gene="CKB1"
/locus_tag="AWJ20_2181"
/db_xref="GeneID:30034064"
mRNA <605132..>606229
/gene="CKB1"
/locus_tag="AWJ20_2181"
/product="casein kinase 2 regulatory subunit CKB1"
/transcript_id="XM_018879111.1"
/db_xref="GeneID:30034064"
CDS 605132..606229
/gene="CKB1"
/locus_tag="AWJ20_2181"
/inference="similar to AA sequence:KEGG_Orthology:K03115"
/note="Beta regulatory subunit of casein kinase 2 (CK2); a
Ser/Thr protein kinase with roles in cell growth and
proliferation; CK2, comprised of CKA1, CKA2, CKB1 and
CKB2, has many substrates including transcription factors
and all RNA polymerases; GO_component: GO:0034456 - UTP-C
complex [Evidence IDA] [PMID 17515605]; GO_component:
GO:0005956 - protein kinase CK2 complex [Evidence IEA];
GO_component: GO:0005956 - protein kinase CK2 complex
[Evidence IDA] [PMID 8135547]; GO_function: GO:0019887 -
protein kinase regulator activity [Evidence IEA];
GO_function: GO:0019887 - protein kinase regulator
activity [Evidence IMP] [PMID 11827175]; GO_function:
GO:0019887 - protein kinase regulator activity [Evidence
IPI] [PMID 8135547]; GO_function: GO:0030291 - protein
serine/threonine kinase inhibitor activity [Evidence IDA]
[PMID 18265947]; GO_process: GO:0006974 - cellular
response to DNA damage stimulus [Evidence IDA] [PMID
11551505]; GO_process: GO:0006468 - protein
phosphorylation [Evidence IMP] [PMID 16607517];
GO_process: GO:0006468 - protein phosphorylation [Evidence
IDA] [PMID 16952051]; GO_process: GO:0045859 - regulation
of protein kinase activity [Evidence IEA]; GO_process:
GO:0006356 - regulation of transcription from RNA
polymerase I promoter [Evidence IDA] [PMID 11551505];
GO_process: GO:0006359 - regulation of transcription from
RNA polymerase III promoter [Evidence IDA] [PMID
11551505]"
/codon_start=1
/product="casein kinase 2 regulatory subunit CKB1"
/protein_id="XP_018737058.1"
/db_xref="GeneID:30034064"
/translation="MSNLRDTPEKQPQRQQQATAAAAAAFRQVDRPGASNASASSLAS
STKNASNVKREPTLSSTQQPPSNIEEDEEEEEEEEEIQDGTAGATGAAGDKYNFMFDE
SDDASSGSLDFESWIKSFCSIVGHEFFAEVSEEFIEDDFNLTGLGPLIPHYKEALELI
LDLEPETPPKLPTIPIIEQSAEQLYGMIHARFILSRPGLQIMAQKFHYQQFGTCPRYF
CHGTGLLPVGRHDMPGFETVRLYCPCCMDIYVPPNSRFLSIDGAFFGTSFPGLFLKAY
PEIEKECARRRKQQGQFELTIYGFKIAESSKSGSRMKWLRQVPVNDSELDEHVSEIQD
GARSISSSSHNNPTNTNDNESMMSISMNGET"
gene complement(<606784..>607383)
/gene="JAC1"
/locus_tag="AWJ20_2182"
/db_xref="GeneID:30034065"
mRNA complement(<606784..>607383)
/gene="JAC1"
/locus_tag="AWJ20_2182"
/product="Jac1p"
/transcript_id="XM_018879112.1"
/db_xref="GeneID:30034065"
CDS complement(606784..607383)
/gene="JAC1"
/locus_tag="AWJ20_2182"
/inference="similar to AA sequence:KEGG_Orthology:K04082"
/note="Specialized J-protein that functions in Fe-S
cluster biogenesis; functions with Hsp70 in Fe-S cluster
biogenesis in mitochondria; involved in iron metabolism;
contains a J domain typical to J-type chaperones;
localizes to the mitochondrial matrix; GO_component:
GO:0005759 - mitochondrial matrix [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 11278728]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0051087 - chaperone binding [Evidence IEA];
GO_function: GO:0051087 - chaperone binding [Evidence IMP]
[PMID 11278728]; GO_function: GO:0051087 - chaperone
binding [Evidence ISS] [PMID 9813017]; GO_process:
GO:0009060 - aerobic respiration [Evidence IMP] [PMID
9813017]; GO_process: GO:0016226 - iron-sulfur cluster
assembly [Evidence IMP] [PMID 11278728]; GO_process:
GO:0006457 - protein folding [Evidence IEA]; GO_process:
GO:0051259 - protein oligomerization [Evidence IEA];
GO_process: GO:0006950 - response to stress [Evidence
IEA]"
/codon_start=1
/product="Jac1p"
/protein_id="XP_018737059.1"
/db_xref="GeneID:30034065"
/translation="MRTVSVSTRLLQTFTKRFATKAGGESGYYSFFPQSFPNGPPPKG
PFEINAKQLKQEFLKLQAQTHPDKAGSVDPNNELRKKFELRSSQLNIAYKSLLNPLDR
AQHLLLLRGIDALSERDSLQDEDLLMDVLMAREAIADSNSREDLLSLQQENDKRLSES
ETALISAFSEDDLESAKNETIRLSYWQNIKNQISDALNQ"
gene complement(<609663..>611222)
/gene="HXT13"
/locus_tag="AWJ20_2183"
/db_xref="GeneID:30034066"
mRNA complement(<609663..>611222)
/gene="HXT13"
/locus_tag="AWJ20_2183"
/product="hexose transporter HXT13"
/transcript_id="XM_018879113.1"
/db_xref="GeneID:30034066"
CDS complement(609663..611222)
/gene="HXT13"
/locus_tag="AWJ20_2183"
/inference="similar to AA sequence:KEGG_Orthology:K08139"
/codon_start=1
/product="hexose transporter HXT13"
/protein_id="XP_018737060.1"
/db_xref="GeneID:30034066"
/translation="MTIEEKNEVHMVEDIRPEGETMEDVLPDYGKFAFLRVPHLLRLN
LLLLLCMISSTNNGYDGSMLNGLQSMDSWQDTFNHPKGQHLGGLANGMMFGGIATWPI
LPYITDRWGRKLPIAGGCVLIIVGAIIQAVSNDYAVFLVGRLILGFGSSFCTGCSPLL
LAESSYPAHRHVVTAIYNTLWYLGSIIAAWLTLGTSYVTTTWSWRIPCICQGFLPLIQ
LLMIYWVPESPRYLIDKHRYAEARALLVKHHAGGDVNSKLVDYEMAEISSALELEKIQ
NETSYLDFLKTKANKHRLFLIIMIPINMQMSGNGLVSYYLNLVLDSIGITDQRQQLYL
NGGLQIYNFFWAVFWTFFVESLGRRRLFLISFSGMLVTYIIWTALSGAGTPSNFANKS
LANGVLAMIFLYYFCYGIALNGPAVLYYTEILPYQLRAKGLNITQIVTTLTLIYNGFV
NPVAMDAIGWKYYIVWCCVIAVELVICYFFYPETKGHTLETVGSVFGEEVHVTSDLGV
GLGRENEKPVA"
gene <615264..>616211
/locus_tag="AWJ20_2184"
/db_xref="GeneID:30034067"
mRNA <615264..>616211
/locus_tag="AWJ20_2184"
/product="hypothetical protein"
/transcript_id="XM_018879114.1"
/db_xref="GeneID:30034067"
CDS 615264..616211
/locus_tag="AWJ20_2184"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737061.1"
/db_xref="GeneID:30034067"
/translation="MTSPKYNCLTQEQVDHFLEKGYVVIHDAFSKEHAAWMLKDVWVR
LGLDPNNRESWTKPRIHMPKQRSILVSEFAPKAWDAIKDLLGGDENIADAGKYWNDSL
IVNFGRDQFEPHLVSGDPKQLDNWHTDGNFFRHFLDSPEQALLVIPIFSDEIKPKGGA
TYIAPDSIPHIAKLLDDHPEGLLPGKPFNYPELVNKCNEFVEVTGKVGDVVLMHPFMM
HSASPNQLRIPRFITNPPVSLKTPFRFDRPLDQLNLVEQKTLKSLGKSSPYHFKPTAD
RVIFPSEQHDMWIRMQNEEKQRLKDYGTLESFTAIGVKP"
gene complement(<616842..>617744)
/gene="YHI9"
/locus_tag="AWJ20_2185"
/db_xref="GeneID:30034068"
mRNA complement(<616842..>617744)
/gene="YHI9"
/locus_tag="AWJ20_2185"
/product="Yhi9p"
/transcript_id="XM_018879115.1"
/db_xref="GeneID:30034068"
CDS complement(616842..617744)
/gene="YHI9"
/locus_tag="AWJ20_2185"
/note="hypothetical protein; null mutant is defective in
unfolded protein response; possibly involved in a membrane
regulation metabolic pathway; member of the PhzF
superfamily, though most likely not involved in phenazine
production; GO_component: GO:0005575 - cellular_component
[Evidence ND]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016853 -
isomerase activity [Evidence IEA]; GO_function: GO:0003674
- molecular_function [Evidence ND]; GO_process: GO:0009058
- biosynthetic process [Evidence IEA]; GO_process:
GO:0030968 - endoplasmic reticulum unfolded protein
response [Evidence IMP] [PMID 16582425]"
/codon_start=1
/product="Yhi9p"
/protein_id="XP_018737062.1"
/db_xref="GeneID:30034068"
/translation="MTTAPFTTVDVFSDTRFLGNQLAIVELAGENTDLSTDNLQQIAR
EFNYSETIYIYPPSTANTYPVRIFTPDTELPFAGHPTIGAADHVIGLVTAGTSSATLV
PPAGPIPVQKSSDGVVRAHIPHNVHFHKATAKDLPDPVGFSSDPVIAEAERNAPLFSI
VKGLSFALIRLPSVEVLSRVKMSSTPLNLAPLLDHEWNSGFVGQLYYVLEDEHTVRAR
MILLDQFEDPATGSANSALGAYLALTTGKNQNIQVTQGVEIGRRSVIGVEVELDGDKI
NTLILSGKTVKVTKGDIIVSADKL"
gene <618341..>619315
/gene="HIS6"
/locus_tag="AWJ20_2186"
/db_xref="GeneID:30034069"
mRNA <618341..>619315
/gene="HIS6"
/locus_tag="AWJ20_2186"
/product="imidazole-4-carboxamide isomerase HIS6"
/transcript_id="XM_018879116.1"
/db_xref="GeneID:30034069"
CDS 618341..619315
/gene="HIS6"
/locus_tag="AWJ20_2186"
/inference="similar to AA sequence:KEGG_Orthology:K01814"
/codon_start=1
/product="imidazole-4-carboxamide isomerase HIS6"
/protein_id="XP_018737063.1"
/db_xref="GeneID:30034069"
/translation="MLDSSAARGATGSGAEPQPPEAETPLPAAGLILSFRICTRYMQH
EETEDRVISRVIKYQIRRAMTKFRGCIDIHNGQVKQIVGGTLTSAESQLKTNFVATQP
AEYFGRLYRKNEVYGTHVIKLGPGCDEAAEEALAEWPGQLQVGGGITAENAQSWIQEK
KASKVIVTSYLFPDGKLSIPRLEKLVSLVGKEHLVIDLSCRKKIVDGEPRWIVAMNRW
QTLTDTEVNKSTLDELSRYCSEFLIHAADVEGLCRGIDEDLVAALGNWVTIPTVYAGG
AKSLNDLELVAKLSHGKVDLTFGSALDIFGGDKVAFSSCVAWNQSQSQ"
gene complement(<619501..>620109)
/gene="RPB3"
/locus_tag="AWJ20_2187"
/db_xref="GeneID:30034070"
mRNA complement(<619501..>620109)
/gene="RPB3"
/locus_tag="AWJ20_2187"
/product="DNA-directed RNA polymerase II core subunit
RPB3"
/transcript_id="XM_018879117.1"
/db_xref="GeneID:30034070"
CDS complement(619501..620109)
/gene="RPB3"
/locus_tag="AWJ20_2187"
/inference="similar to AA sequence:KEGG_Orthology:K03011"
/note="RNA polymerase II third largest subunit B44; part
of central core; similar to prokaryotic alpha subunit;
GO_component: GO:0005665 - DNA-directed RNA polymerase II,
core complex [Evidence IDA] [PMID 1331084]; GO_component:
GO:0005665 - DNA-directed RNA polymerase II, core complex
[Evidence IDA] [PMID 2183013]; GO_component: GO:0005665 -
DNA-directed RNA polymerase II, core complex [Evidence
IDA] [PMID 2186966]; GO_component: GO:0005654 -
nucleoplasm [Evidence IDA] [PMID 15520468]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_function:
GO:0003677 - DNA binding [Evidence IEA]; GO_function:
GO:0003899 - DNA-directed RNA polymerase activity
[Evidence IEA,IEA]; GO_function: GO:0001055 - RNA
polymerase II activity [Evidence IDA] [PMID 8288647];
GO_function: GO:0003968 - RNA-directed RNA polymerase
activity [Evidence IDA] [PMID 18004386]; GO_function:
GO:0046872 - metal ion binding [Evidence IEA];
GO_function: GO:0046983 - protein dimerization activity
[Evidence IEA]; GO_process: GO:0006369 - termination of
RNA polymerase II transcription [Evidence IMP] [PMID
16537912]; GO_process: GO:0006366 - transcription from RNA
polymerase II promoter [Evidence IMP] [PMID 2685562];
GO_process: GO:0006351 - transcription, DNA-templated
[Evidence IEA]"
/codon_start=1
/product="DNA-directed RNA polymerase II core subunit
RPB3"
/protein_id="XP_018737064.1"
/db_xref="GeneID:30034070"
/translation="MEIYTRDLVVHLPKDGRRDESIGQPVFSDPAKKGVLICKLRKHQ
ELRLKCIAKKGIAKEHAKWSPVAAVGFEYDPWNKLHHTDYWYEESIEEEWPKSKNADW
EEPPKENEPFDYTAKPDKFYYTVETVGSLKPNEVVERGIRVLQNKLAEIVLAVDKLDG
RGPGMDLGGGATGRHGGATDYGGATTNMGNYGSPRMDGNQWF"
gene complement(<621866..>623555)
/locus_tag="AWJ20_2188"
/db_xref="GeneID:30034071"
mRNA complement(join(<621866..622651,623514..>623555))
/locus_tag="AWJ20_2188"
/product="hypothetical protein"
/transcript_id="XM_018879118.1"
/db_xref="GeneID:30034071"
CDS complement(join(621866..622651,623514..623555))
/locus_tag="AWJ20_2188"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737065.1"
/db_xref="GeneID:30034071"
/translation="MSMYIWSHISHVRVSANNYAGQQPSVTPGYPNYASSGTVPQQPA
AATSSVASVPAPATAPVNQGVPSVPAVPQQATETASAVPAVPAVSTQTPAASNSVPAA
ATAPSVSSNNGGDASVQGTHAGRGDSGASSRPKHNNRNGQGQKPKVVLPEFNFDASNA
KFSKEDITKEESVIPKAPAEGFYDKKSSFFDNISSTTKEQIEGSSDNKTAYQRRGEER
QLNMETFGQAESNHGNWRGGRGGRGRGRGGHRGRGNGNWRGGRGGFNNNNNNSNNFS"
gene complement(<623783..>623877)
/locus_tag="AWJ20_2189"
/db_xref="GeneID:30034072"
tRNA complement(<623783..>623877)
/locus_tag="AWJ20_2189"
/product="tRNA-Phe"
/db_xref="GeneID:30034072"
gene <625411..>625941
/gene="NAT3"
/locus_tag="AWJ20_2190"
/db_xref="GeneID:30034074"
mRNA <625411..>625941
/gene="NAT3"
/locus_tag="AWJ20_2190"
/product="Nat3p"
/transcript_id="XM_018879120.1"
/db_xref="GeneID:30034074"
CDS 625411..625941
/gene="NAT3"
/locus_tag="AWJ20_2190"
/inference="similar to AA sequence:KEGG_Orthology:K17972"
/note="Catalytic subunit of the NatB N-terminal
acetyltransferase; NatB catalyzes acetylation of the
amino-terminal methionine residues of all proteins
beginning with Met-Asp or Met-Glu and of some proteins
beginning with Met-Asn or Met-Met; GO_component:
GO:0031416 - NatB complex [Evidence IDA] [PMID 12783868];
GO_component: GO:0005737 - cytoplasm [Evidence IEA,IEA];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_function: GO:0008080 - N-acetyltransferase
activity [Evidence IEA]; GO_function: GO:0004596 - peptide
alpha-N-acetyltransferase activity [Evidence IEA];
GO_function: GO:0004596 - peptide
alpha-N-acetyltransferase activity [Evidence IMP,ISS]
[PMID 10545125]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_function: GO:0016746 -
transferase activity, transferring acyl groups [Evidence
IEA]; GO_process: GO:0017196 - N-terminal
peptidyl-methionine acetylation [Evidence IMP] [PMID
10545125]; GO_process: GO:0007010 - cytoskeleton
organization [Evidence IGI,IMP] [PMID 12808144];
GO_process: GO:0000001 - mitochondrion inheritance
[Evidence IGI,IMP] [PMID 12808144]"
/codon_start=1
/product="Nat3p"
/protein_id="XP_018737066.1"
/db_xref="GeneID:30034074"
/translation="MTTPASRTSQTLIADRPESKMTSITPLKATDLLSLNSINLDVLT
ENYHLGFYLQYLSDWPAMFFKSTNPADRCTGYMMGKSEGRGLEWHSHITAVTVDCDYR
RLGIAKDLVDHLVTCSEESTQDCYFMDLYVRATNRLAIDMYKKFGFSVFRRVVAYYSM
GEPSDDGEDAFGKYHQ"
gene <626823..>627266
/locus_tag="AWJ20_2191"
/db_xref="GeneID:30034075"
mRNA <626823..>627266
/locus_tag="AWJ20_2191"
/product="60S ribosomal protein L12"
/transcript_id="XM_018879121.1"
/db_xref="GeneID:30034075"
CDS 626823..627266
/locus_tag="AWJ20_2191"
/codon_start=1
/product="60S ribosomal protein L12"
/protein_id="XP_018737067.1"
/db_xref="GeneID:30034075"
/translation="MSRLTVDSAADGLSSTQNLLDSTRQLLGKGLGSHLTGNVDDLIE
GNATVVLDVLLLLSVSWGFLKSSDEERRGRGNNRDSGLTVLDGQLNSDTDTLEVLGGL
GDIFTDLLGGETKRTDLGGKSGRSTNFTTDSTEEEDFNFVGVDLN"
gene complement(<628197..>631256)
/gene="UBA1"
/locus_tag="AWJ20_2192"
/db_xref="GeneID:30034076"
mRNA complement(<628197..>631256)
/gene="UBA1"
/locus_tag="AWJ20_2192"
/product="E1 ubiquitin-activating protein UBA1"
/transcript_id="XM_018879122.1"
/db_xref="GeneID:30034076"
CDS complement(628197..631256)
/gene="UBA1"
/locus_tag="AWJ20_2192"
/inference="similar to AA sequence:KEGG_Orthology:K03178"
/note="Ubiquitin activating enzyme (E1); involved in
ubiquitin-mediated protein degradation and essential for
viability; protein abundance increases in response to DNA
replication stress; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 22842922]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 7629121]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 22842922];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
7629121]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_function: GO:0008641 - small
protein activating enzyme activity [Evidence IEA];
GO_function: GO:0004839 - ubiquitin activating enzyme
activity [Evidence IDA] [PMID 1989885]; GO_process:
GO:0006464 - cellular protein modification process
[Evidence IEA]; GO_process: GO:0016567 - protein
ubiquitination [Evidence IEA]; GO_process: GO:0016567 -
protein ubiquitination [Evidence IDA] [PMID 1989885]"
/codon_start=1
/product="E1 ubiquitin-activating protein UBA1"
/protein_id="XP_018737068.1"
/db_xref="GeneID:30034076"
/translation="MNVDAEAPSGEIDEGLYSRQLYVLGRDAMKRMAQSNVLIVGLKG
LGAEIAKNVTLAGVKSIALYDPGAVKIEDLSSQFFLYESDVGSGKSRAELTAPRLSEL
NQYVPVSVVDGELTEDVLAKFQVVVLTNTDIETQVKINSFTHANNINFISTDIRGLFG
SVFVDFGDKFQVFDPTGEEPQSGIISAIEPDGTVAALDETRHGLEDGDWVKFTEVEGL
EALNDGQPREVKVTGPYTFNIGDVSGLGSYVKGGIFTQVKKPLELSFQSLADQLAKPE
YLVTDFAKFDRPAQLHIGFLALHGFANQHGGELPRPYNKEDANQVAHLAKSVAAQYPE
VLGETAIDEDLIRKLSFQSRGDYAPLAAVFGGLVAQEVLKACSGKFVPVRQFSYFDSL
ESLPSKEEITEEDAAPSGSRYDGNVAIFGKKFVEKLRNLKVFLVGSGAIGCEMLKNWA
LLGVGSGPEGRIIVTDNDSIEKSNLNRQFLFRPSDVGKQKSSVAAAAVVKINPDLKGK
IEARSDKVGPETEDIFNDKFWNSLDFVTNALDNVDARTYVDRRCVFFRKPLLESGTLG
TKGNVQVVYPDLTESYSSSQDPPEKSIPLCTLRSFPNKIDHTIAWAKSLFQGYFVDSA
ENVNLYLSQPNFVESHLKQSGDQKTILETIYSFLVSDRPLTFEQCIAWARLEFEKKFN
NDIQQLLYNFPKDAVTSTGTPFWSGPKRAPTPLKFDINNEDHFNFIVAGANLHAFNYG
LKSDVSTREEYTRVLQTISVPEFKPRSGVSIQANDNDPVPEANLGDDQDEIQKLAQSL
PPPASLAGFRLIPVEFEKDDDTNYHIDFITAGSNLRALNYGIDPADKSKTKFIAGRII
PAIATTTSLVTGLVCLELTKVVDGENKTIEDFKNGFINLALPFFGFSEPIASAKIKYN
DKEIDKIWGRFDVHNKDITLKELIEHFKTEEGLEINMLSTGTTLLYASFHPASKKKER
LGLKLRDLVEQVSKKPIPPHTETMIFEIVADDSEGEDVEVPFITLHL"
gene <632569..>632672
/locus_tag="AWJ20_2193"
/db_xref="GeneID:30034077"
tRNA <632569..>632672
/locus_tag="AWJ20_2193"
/product="tRNA-Ser"
/db_xref="GeneID:30034077"
gene complement(<635302..>636210)
/locus_tag="AWJ20_2194"
/db_xref="GeneID:30034078"
mRNA complement(join(<635302..635914,636185..>636210))
/locus_tag="AWJ20_2194"
/product="hypothetical protein"
/transcript_id="XM_018879123.1"
/db_xref="GeneID:30034078"
CDS complement(join(635302..635914,636185..636210))
/locus_tag="AWJ20_2194"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737069.1"
/db_xref="GeneID:30034078"
/translation="MLPAPTLWKLETAPVGAPQEVAGAELAGGGGAADEAAADEAAAD
EAAADEAAADEAAADEAAADEAAADEAAADEAAADEAAADEAAAEEAAAEEAAAEEAA
ADEAAAEEAAADEAAADEAAAEEAAAEEAAAEEAAADEAAADEAAAEEAAADDEGAAD
DEGAADDEQALLDGLGLEAIVTEYLLAAAAKAASILEKSLITSAKTEQSNLS"
gene complement(<637869..>637979)
/locus_tag="AWJ20_2195"
/db_xref="GeneID:30034079"
tRNA complement(<637869..>637979)
/locus_tag="AWJ20_2195"
/product="tRNA-Leu"
/db_xref="GeneID:30034079"
gene <638621..>639715
/locus_tag="AWJ20_2196"
/db_xref="GeneID:30034080"
mRNA <638621..>639715
/locus_tag="AWJ20_2196"
/product="reverse transcriptase"
/transcript_id="XM_018879124.1"
/db_xref="GeneID:30034080"
CDS 638621..639715
/locus_tag="AWJ20_2196"
/codon_start=1
/product="reverse transcriptase"
/protein_id="XP_018737070.1"
/db_xref="GeneID:30034080"
/translation="MHVDNQRTSKKRQLRLVLPSGERRQASPTLKLLGVTLDSQWKFS
KHVEAIAIKGKMVLGIIRRLGGITWGVTGASMRNLYQGCVRPILEYASPVWYPKITKQ
EREQLQRIQNTGLRAILGGYHQTPIDCLHRDTDIMPLEQRYDTLQDNYIVRLHRNVDP
ENPVNAESTWWRKHMEHNELVQRLYEVLPKEEIFQDRIRRRKPPWKKEDMESESKAWK
VKSELKKKIYQRHHTIWETQYRTSAKGEFYRSYTLPRLYNADHKNPLRYFLNECSKNE
LSKLVQLRTAKGAFGMFFKRFKINNRPHQCECGEEEDVKHLLCECPVTENHRQILRDA
SATLDIKVLLDSKKGLKAVLAFLAKAPQLL"
gene complement(<641223..>644366)
/gene="POL92"
/locus_tag="AWJ20_2197"
/db_xref="GeneID:30034081"
mRNA complement(<641223..>644366)
/gene="POL92"
/locus_tag="AWJ20_2197"
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 3"
/transcript_id="XM_018879125.1"
/db_xref="GeneID:30034081"
CDS complement(641223..644366)
/gene="POL92"
/locus_tag="AWJ20_2197"
/note="allele of CaO19.8194"
/codon_start=1
/product="polyprotein of L1-like non-LTR retrotransposon
Zorro 3"
/protein_id="XP_018737071.1"
/db_xref="GeneID:30034081"
/translation="MFADFDLLLLQEPGLNLGQHPIQHSAWVAVHTQSQTGRINAITY
INKRIYGQSQLKMVGELTTENVVTIQIGQLYITNIYNRISEDIHPVEYYKTLLPQIPE
LIIIAGDFNLHHPMWQSTEHVTREANSWADWVNDNGLLLATVPNVPTHIFGNTIDLTF
TTPNITITPEKSEDVGSDHYLQKWRIDTNPLAIELLQTYGGRYNYKRADWENFEKVLN
SRAAMVTIPKELRGYSNGKSTHKVADRYASKLIGLLCNCLDETVPKLKIVPRSKRWWT
PELRQAKRDVGRARRRARSKPTEENIRLRQQANEEYASKILEAKNTTWNSFLASRRGG
EIYDAHKMIKPREQESVMPALRLETGELAQTFEEQEDELYRGLFPHVTPSTRRVLKVV
KTGQWKSLRTQEIEDAIRDQAPHKAPGPDGVKTVAIKRAWVVKRFRKLLKGLLKYCIR
VGYHPKCFREGITVVIPKRKRDPTLARSYRPITLLSTLGKVLEKIMQRRLTALTSGML
PVDQYGGRHGSSAVEAAHKLVHHAEKRMKRGQVTSVLAIDIKGAFDHVHRDTLLDTMG
GMGLPTAVQNWVYQFMRGRRTSLVIDGKRTKERRIATGIPQGSPISPLLFLIYTSPLY
PLIKSMGGKVIGFIDDITIYVSSTRYAENTAKLSNILARCHEWATSAHAFIDYGEKLG
FMHIAKRVTPKGCRRLILPTGERIQATGSLRLLGVTIDRQLKFDEHAKSVISKGQSAL
NIIRRLGGVTRGVTGATMRCLYKSCVRPILEYASPIWYNRIGKGLKEAIQRIQNAALR
SILGAYKPTAIDSLHRDAEIPPVEHRMLETEQYYLVRLHRDLHRYNPHRIRARKLYSG
TILGDRLDELYRTVEATDIKKDRYRLWKPPWASRDSEAISKASSQKKSIRKEIRSQCY
QKWEIEYTQSPKGAFYRSFTAPRLYSTKHANALRPFLYESPRGELSKLVQLRTGMGAM
GSFFQRFRISNRRYDCRCGVEETVEHILRDCPLTEEHRQILREASRELDLPSLLDTRK
GLKAVASFLKANPSLLS"
gene <646666..>646739
/locus_tag="AWJ20_2198"
/db_xref="GeneID:30034082"
tRNA <646666..>646739
/locus_tag="AWJ20_2198"
/product="tRNA-Ala"
/db_xref="GeneID:30034082"
gene <648850..>649974
/gene="BAT1"
/locus_tag="AWJ20_2199"
/db_xref="GeneID:30034083"
mRNA <648850..>649974
/gene="BAT1"
/locus_tag="AWJ20_2199"
/product="branched-chain-amino-acid transaminase BAT1"
/transcript_id="XM_018879126.1"
/db_xref="GeneID:30034083"
CDS 648850..649974
/gene="BAT1"
/locus_tag="AWJ20_2199"
/inference="similar to AA sequence:KEGG_Orthology:K00826"
/note="Mitochondrial branched-chain amino acid (BCAA)
aminotransferase; preferentially involved in BCAA
biosynthesis; homolog of murine ECA39; highly expressed
during logarithmic phase and repressed during stationary
phase; BAT1 has a paralog, BAT2, that arose from the whole
genome duplication; GO_component: GO:0005759 -
mitochondrial matrix [Evidence IEA]; GO_component:
GO:0005759 - mitochondrial matrix [Evidence IDA] [PMID
8798704]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0052656 -
L-isoleucine transaminase activity [Evidence IEA];
GO_function: GO:0052654 - L-leucine transaminase activity
[Evidence IEA]; GO_function: GO:0052655 - L-valine
transaminase activity [Evidence IEA]; GO_function:
GO:0004084 - branched-chain-amino-acid transaminase
activity [Evidence IEA,IEA]; GO_function: GO:0004084 -
branched-chain-amino-acid transaminase activity [Evidence
IDA] [PMID 8798704]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0008483 -
transaminase activity [Evidence IEA]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0019509 - L-methionine salvage from
methylthioadenosine [Evidence IEA]; GO_process: GO:0009082
- branched-chain amino acid biosynthetic process [Evidence
IEA]; GO_process: GO:0009082 - branched-chain amino acid
biosynthetic process [Evidence IDA,IMP] [PMID 8798704];
GO_process: GO:0009083 - branched-chain amino acid
catabolic process [Evidence IMP] [PMID 8702755];
GO_process: GO:0009081 - branched-chain amino acid
metabolic process [Evidence IEA]; GO_process: GO:0008652 -
cellular amino acid biosynthetic process [Evidence IEA];
GO_process: GO:0009097 - isoleucine biosynthetic process
[Evidence IEA]; GO_process: GO:0009098 - leucine
biosynthetic process [Evidence IEA]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0009099 - valine biosynthetic process [Evidence IEA]"
/codon_start=1
/product="branched-chain-amino-acid transaminase BAT1"
/protein_id="XP_018737072.1"
/db_xref="GeneID:30034083"
/translation="MTVTYSELSAKGLVIEKHPNPTQFENHSDLPSSRATDYLLEVTW
TIDQGWGTPSLHPYGPLSLDPASSVLHYATTCFEGMKAYRSFDGKKVTLFRPLDNINR
LNDSCARASLPQLEPNAALALLEKFVEMEARFIAPGSFIYLRPMVMGTNAHLGLTTPT
EAKFLIMASMMPKLNAAPLKLCSSPPDQIRAWKGGFGYAKLGANYGPTLKANNEAIKN
GFSQVLWLMGEEGIVTEAGGANFFAVIKNEKTGKDEIMTCPLTTGVILPGVTRRSVLE
LLRNKYTSEGVEVNEKEFTISDIEKASDEGRLVEAFAVGTAYFIAPVQTIRTPAGKDI
SVPMSIGTSGDYAHYIKETLSQIMWSKVEHPWSHPVASAQ"
gene complement(<650082..>651068)
/gene="ZTA1"
/locus_tag="AWJ20_2200"
/db_xref="GeneID:30034086"
mRNA complement(<650082..>651068)
/gene="ZTA1"
/locus_tag="AWJ20_2200"
/product="Zta1p"
/transcript_id="XM_018879129.1"
/db_xref="GeneID:30034086"
CDS complement(650082..651068)
/gene="ZTA1"
/locus_tag="AWJ20_2200"
/inference="similar to AA sequence:KEGG_Orthology:K00344"
/note="NADPH-dependent quinone reductase; GFP-tagged
protein localizes to the cytoplasm and nucleus; has
similarity to E. coli quinone oxidoreductase and to human
zeta-crystallin; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 14562095]; GO_function:
GO:0032440 - 2-alkenal reductase [NAD(P)] activity
[Evidence IDA] [PMID 21276778]; GO_function: GO:0017091 -
AU-rich element binding [Evidence IDA] [PMID 17497241];
GO_function: GO:0017091 - AU-rich element binding
[Evidence IDA] [PMID 19007762]; GO_function: GO:0003960 -
NADPH:quinone reductase activity [Evidence IEA];
GO_function: GO:0003960 - NADPH:quinone reductase activity
[Evidence IDA] [PMID 17497241]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0034599 - cellular response to oxidative stress
[Evidence IMP] [PMID 17497241]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="Zta1p"
/protein_id="XP_018737073.1"
/db_xref="GeneID:30034086"
/translation="MAIPTTQKAIRIHETGGPEVVKLEEIPVPEITETQVLVKNEYAG
VNFIETYFRKGIYKAQLPVTLGREGAGEVVKVGSKVSNYSVGDKVGFVGSAFAEYVAI
EAVGKALKITKIPKGLDTKTVGAGLIQGLTALVLTTQSVALKKGDSVLVHAAAGGTGA
LIVQLAKAFGATVIGTTSSAEKAKIAKAAGADHVINYRTEDVTARVKEITNGAGVTGT
LDGVGKDTFNISIASTANSGTVASFGNASGVVPEFSLLSLERNIKVQRPSLFNALTTT
EDWEALTKQFFDLLLSGDLKLDISKVYPLAETSQAISDLESGKTTGKLVIQI"
gene complement(<651923..>652004)
/locus_tag="AWJ20_2201"
/db_xref="GeneID:30034087"
tRNA complement(<651923..>652004)
/locus_tag="AWJ20_2201"
/product="tRNA-Thr"
/db_xref="GeneID:30034087"
gene complement(<653395..>655200)
/locus_tag="AWJ20_2202"
/db_xref="GeneID:30034088"
mRNA complement(<653395..>655200)
/locus_tag="AWJ20_2202"
/product="hypothetical protein"
/transcript_id="XM_018879130.1"
/db_xref="GeneID:30034088"
CDS complement(653395..655200)
/locus_tag="AWJ20_2202"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737074.1"
/db_xref="GeneID:30034088"
/translation="MAAQYVSNISPLRLVSYSFSDEGEFPHFATVVTEKASNAAIAAA
AASASASFGINTYSNGTNPGGPDDHISYLDLSFLTLQAVSQTVIICLFGYFAARAKIL
TPAVQRQVSMLNVQIFTPCLIFSKLASSLSLSALVDIAIIPVLFVITTGISMLCAKIT
SRIFRFNLRESNFVTAMAVFGNSNSLPVSLTVSLAYTLPSLAWPDIPDDNQDDVASRG
ILYLLIFQQLGQILRWSWGYNTLLAKPKDEDEFCEEDEDDEISSIEEGAVANESAHVG
TAKNKHSVNRKRRKQRGVDYSAVNADDVESQLGPQKSPVIITEDNASSPSSSSSSSVV
ETGFSTRVGSENGLAELGSESAPLIVGDENTVSLASPTSHTSHVVSRISSNATVLSRN
DESCNASTPLLPQTLSRKIISSVQKVFMSFLSFMNPPLWSMVIAIIVASIPAAKHELF
ETNGFVNHAITGAVTQVGGIAIPMILVVLGSNLAPDESTAPASKRHSSIVFASLISRM
ILPSIFLLPIIALSVKYIKLSILDDPIFLLVAFILTISPPAIQLSQISQLNGVFEKEM
ASVLFWGYVVLTLPSTIAIVVASLEVLDWAGTTRS"
gene complement(<657616..>658770)
/locus_tag="AWJ20_2203"
/db_xref="GeneID:30034089"
mRNA complement(<657616..>658770)
/locus_tag="AWJ20_2203"
/product="hypothetical protein"
/transcript_id="XM_018879131.1"
/db_xref="GeneID:30034089"
CDS complement(657616..658770)
/locus_tag="AWJ20_2203"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737075.1"
/db_xref="GeneID:30034089"
/translation="MYPKIEGLDPDETKRTFQGVYAHSARWDPETPLEDKDVIVIGNG
CSGAQIVPACAKTAKSVTQFFRAKHWLVPTPKMDPLKVRDKVNNVLGVRLSRMLLFLF
LEAQFPMFSNDGYWANKVRDFNTNKARTHMLKTAPQKYHDMIMPDFKIGCKRRIIDYG
YLQSLHNENVLLSDDPIVSVSEHHVHTKSGKSYHADVIFAATGFDVQSSSGNIPIYGL
DKEENITEFWDRTGVSAYNVSQVAGYPNLFYLLGPNAGTGHTSALLQIENTLTYIDNV
AGYVLRGEASSVDVKLTAYLEWKREINEASKQTVQMKGGCSSWYIKNGTNSVFYPWSQ
IRYWWRAVWPKYEDQTYRWTRNDVIQTKRRRRHTLLAVLLSIILKFTYFK"
gene complement(<659778..>660935)
/gene="JLP1"
/locus_tag="AWJ20_2204"
/db_xref="GeneID:30034090"
mRNA complement(<659778..>660935)
/gene="JLP1"
/locus_tag="AWJ20_2204"
/product="Jlp1p"
/transcript_id="XM_018879132.1"
/db_xref="GeneID:30034090"
CDS complement(659778..660935)
/gene="JLP1"
/locus_tag="AWJ20_2204"
/note="Fe(II)-dependent sulfonate/alpha-ketoglutarate
dioxygenase; involved in sulfonate catabolism for use as a
sulfur source; contains sequence that resembles a J domain
(typified by the E. coli DnaJ protein); induced by sulphur
starvation; GO_component: GO:0005575 - cellular_component
[Evidence ND]; GO_function: GO:0051213 - dioxygenase
activity [Evidence IEA]; GO_function: GO:0046872 - metal
ion binding [Evidence IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0000907 - sulfonate dioxygenase activity [Evidence IDA]
[PMID 10482536]; GO_process: GO:0046306 - alkanesulfonate
catabolic process [Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0044273 - sulfur compound catabolic process
[Evidence IMP] [PMID 10482536]"
/codon_start=1
/product="Jlp1p"
/protein_id="XP_018737076.1"
/db_xref="GeneID:30034090"
/translation="MSPPTAVEPPKPVILDSKQFKKAGGDYKGLSPFFDKPTEGPSYQ
PNTKPLKASGALDEKYSFSEVSPVIGREYTGAQLRDILQDQELLRDLAITVSRRGVVF
FRNQALTAEEQKELADKLGVLTGKPSTSKLHIHPRTPGGGFLKPDGSEIDPEITIISS
KLQKSLYENRNRYKENASRGWHADFAFEPIPSTYSVLKIIEKPEVGGDTLWISGNGLY
DKLSPSFREYLETLYATFQGSGIEKAAQGKFDLFAGPRGAPENVGTELKAVHPAIRTN
PVTGWKSLYAAGAQFSHFNDITEDESGALQRYLKSVLIGSHDLQARFKWGKNDVAIWD
NRSVFHSATNDYFETAVERLGVRTVGLGERPYFDPKSKSRSDDLRESGLSV"
gene <662044..>663009
/locus_tag="AWJ20_2205"
/db_xref="GeneID:30034091"
mRNA <662044..>663009
/locus_tag="AWJ20_2205"
/product="putative epoxide hydrolase"
/transcript_id="XM_018879133.1"
/db_xref="GeneID:30034091"
CDS 662044..663009
/locus_tag="AWJ20_2205"
/note="similar to several putative epoxide hydrolases; has
match to alpha/beta hydrolase fold; allele of CaO19.6709"
/codon_start=1
/product="putative epoxide hydrolase"
/protein_id="XP_018737077.1"
/db_xref="GeneID:30034091"
/translation="MSRFEVKLHANNLTFSAYSSYTEHQLAQVTPDRIILLLHGFPDT
RQSFDEIWPILEKKYPAPSTLLLAPSLRGYEPETVLPSRLSYTPTDIASDVIGWIKEI
IGDKKIPVHLIGHDWGALATFRTASLQPDLITSISTLAIPYMVNLSPLELLWHVPEQI
YLSSYMVRMQTSYFYGSRLYEDAVTTVVGAQGKERKVLPYIEYLWKYWSPGWKFTQDE
LADFCEAVQKPGVVDATTGYYRSLAAKRDRYWRVDFNKVPGLLIGGRDDGCMSYRLYD
LQREKLAPYGENVKIALVDKVGHFMHRENPVTIAELCIDWIDSHE"
gene <664660..>664944
/gene="UGA4"
/locus_tag="AWJ20_2206"
/db_xref="GeneID:30034092"
mRNA <664660..>664944
/gene="UGA4"
/locus_tag="AWJ20_2206"
/product="Uga4p"
/transcript_id="XM_018879134.1"
/db_xref="GeneID:30034092"
CDS 664660..664944
/gene="UGA4"
/locus_tag="AWJ20_2206"
/note="GABA (gamma-aminobutyrate) permease; serves as a
GABA transport protein involved in the utilization of GABA
as a nitrogen source; catalyzes the transport of
putrescine and delta-aminolevulinic acid (ALA); localized
to the vacuolar membrane; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
14985124]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA]; GO_component: GO:0005774 - vacuolar membrane
[Evidence IEA]; GO_component: GO:0005773 - vacuole
[Evidence IEA]; GO_function: GO:0015171 - amino acid
transmembrane transporter activity [Evidence IEA];
GO_function: GO:0015495 - gamma-aminobutyric acid:proton
symporter activity [Evidence IGI] [PMID 8455553];
GO_function: GO:0015489 - putrescine transmembrane
transporter activity [Evidence IGI] [PMID 14985124];
GO_process: GO:0003333 - amino acid transmembrane
transport [Evidence IEA]; GO_process: GO:0006865 - amino
acid transport [Evidence IEA]; GO_process: GO:0015812 -
gamma-aminobutyric acid transport [Evidence IGI] [PMID
14985124]; GO_process: GO:0015812 - gamma-aminobutyric
acid transport [Evidence IEP,IGI] [PMID 8455553];
GO_process: GO:0015847 - putrescine transport [Evidence
IGI] [PMID 14985124]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0055085 - transmembrane transport [Evidence IGI] [PMID
8455553]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Uga4p"
/protein_id="XP_018737078.1"
/db_xref="GeneID:30034092"
/translation="MGNNSEEIVRQETLQGVPTTEKPTVTDDDHLLMQLGYKPELRRN
FSVIQVFGIAFSIMSLLPSIASVLALNLPAGGCATWGWLAASVCILTVGM"
gene <665026..>666414
/gene="UGA4"
/locus_tag="AWJ20_2207"
/db_xref="GeneID:30034093"
mRNA <665026..>666414
/gene="UGA4"
/locus_tag="AWJ20_2207"
/product="Uga4p"
/transcript_id="XM_018879135.1"
/db_xref="GeneID:30034093"
CDS 665026..666414
/gene="UGA4"
/locus_tag="AWJ20_2207"
/note="GABA (gamma-aminobutyrate) permease; serves as a
GABA transport protein involved in the utilization of GABA
as a nitrogen source; catalyzes the transport of
putrescine and delta-aminolevulinic acid (ALA); localized
to the vacuolar membrane; GO_component: GO:0000329 -
fungal-type vacuole membrane [Evidence IDA] [PMID
14985124]; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA,IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence ISM] [PMID
12192589]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA]; GO_component: GO:0005774 - vacuolar membrane
[Evidence IEA]; GO_component: GO:0005773 - vacuole
[Evidence IEA]; GO_function: GO:0015171 - amino acid
transmembrane transporter activity [Evidence IEA];
GO_function: GO:0015495 - gamma-aminobutyric acid:proton
symporter activity [Evidence IGI] [PMID 8455553];
GO_function: GO:0015489 - putrescine transmembrane
transporter activity [Evidence IGI] [PMID 14985124];
GO_process: GO:0003333 - amino acid transmembrane
transport [Evidence IEA]; GO_process: GO:0006865 - amino
acid transport [Evidence IEA]; GO_process: GO:0015812 -
gamma-aminobutyric acid transport [Evidence IGI] [PMID
14985124]; GO_process: GO:0015812 - gamma-aminobutyric
acid transport [Evidence IEP,IGI] [PMID 8455553];
GO_process: GO:0015847 - putrescine transport [Evidence
IGI] [PMID 14985124]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0055085 - transmembrane transport [Evidence IGI] [PMID
8455553]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="Uga4p"
/protein_id="XP_018737079.1"
/db_xref="GeneID:30034093"
/translation="MAEMGSALPTSGGLYWWTFKFAPEALKKPLCFLAGYSNTLGLVG
GVVSIDYGFASMVLSVPSIADPHFTPNKYQTYGVFVACVLSHMVIGSVATTVMSRLQT
FCIVINMLVIVITVIAVPIGAKNLRSGHEVFTDTTNLNDWPYGWTWMLSWMSAIWTIG
AFDSCVHLSEEATNAATAVPFGIVLSISMCGVLGFAILAVLISSISDFSAVINTATGQ
PMAQIYLDALGKKWTIAMMIMLFIVQWFMGLSLLVAASRQTWAFSRDGALPLSWWVRK
INMKLQVPVRAVIFDGVLCLVIGLLVLVDTAAALALFSLAPASNGLAWLLPIFCRHVW
FDKDAFKPGPFYLGHFFSRLNGLFASVYLIFVVFVLSMWPASGPDPTPQDMNYTCVIN
GAVWVGSLLYYFLSARKWFEGPQQTLDTEEIHGVDLIATHSQHHHGEGHEHQHQHEQP
QEVFEHAEKTPF"
gene <666928..>668013
/gene="GLE2"
/locus_tag="AWJ20_2208"
/db_xref="GeneID:30034094"
mRNA <666928..>668013
/gene="GLE2"
/locus_tag="AWJ20_2208"
/product="RNA export factor GLE2"
/transcript_id="XM_018879136.1"
/db_xref="GeneID:30034094"
CDS 666928..668013
/gene="GLE2"
/locus_tag="AWJ20_2208"
/inference="similar to AA sequence:KEGG_Orthology:K14298"
/note="RNA export factor associated with the nuclear pore
complex (NPC); associates with NUP116p; required for
polyadenylated RNA export but not for protein import;
homologous to S. pombe Rae1p and human RAE1; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 15210706];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0031965 -
nuclear membrane [Evidence IEA]; GO_component: GO:0005643
- nuclear pore [Evidence IEA,IEA]; GO_component:
GO:0005643 - nuclear pore [Evidence IDA] [PMID 10684247];
GO_component: GO:0005643 - nuclear pore [Evidence IDA]
[PMID 8970155]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0051028 -
mRNA transport [Evidence IEA]; GO_process: GO:0031081 -
nuclear pore distribution [Evidence IMP] [PMID 9725905];
GO_process: GO:0016973 - poly(A)+ mRNA export from nucleus
[Evidence IMP] [PMID 8970155]; GO_process: GO:0000973 -
posttranscriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [Evidence IMP] [PMID 20932479];
GO_process: GO:0015031 - protein transport [Evidence IEA];
GO_process: GO:2000728 - regulation of mRNA export from
nucleus in response to heat stress [Evidence IMP] [PMID
15210706]; GO_process: GO:0000972 -
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery [Evidence IMP] [PMID
20098417]; GO_process: GO:0000972 -
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery [Evidence IMP] [PMID
20932479]; GO_process: GO:0006810 - transport [Evidence
IEA]"
/codon_start=1
/product="RNA export factor GLE2"
/protein_id="XP_018737080.1"
/db_xref="GeneID:30034094"
/translation="MALKLNLDADITVENMPPNDTVSQLDFSPKSNLLSASSWDGIVR
VYSVFSPEVDFNKKELNNANAALQASYITGYTHSQPVLTTGWLQDKYQIISGGCDKLV
KIFDIGTGHVADIGHHEAPVSSVKSVMIGGSPIIVSSSWDKTLSYWDLRQQQAVCSIQ
LDGKALSMDSRDGVIVLVTSDRTTVKIDLSNPTVVSGTFKSRWELQPRVVKCLPGGEC
CIIGGTEGRCVVQYFRDVKSSFSFKCHRTTTGTDVHIYSVNDIAVNPIYGSTLTAGSD
GSLQIWDHIKQCRLSYSGILPGSVSSCAYNNNGSILAYAVYYDWSKGYQFSRPNYPKL
LKLHKIKPETVELWSNKNKIKIKIVYK"
gene complement(<668113..>675045)
/gene="CSF1"
/locus_tag="AWJ20_2209"
/db_xref="GeneID:30034095"
mRNA complement(<668113..>675045)
/gene="CSF1"
/locus_tag="AWJ20_2209"
/product="Csf1p"
/transcript_id="XM_018879137.1"
/db_xref="GeneID:30034095"
CDS complement(668113..675045)
/gene="CSF1"
/locus_tag="AWJ20_2209"
/note="Protein required for fermentation at low
temperature; plays a role in the maturation of secretory
proteins; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0006113 -
fermentation [Evidence IMP] [PMID 10781556]; GO_process:
GO:0051604 - protein maturation [Evidence IMP] [PMID
19433630]"
/codon_start=1
/product="Csf1p"
/protein_id="XP_018737081.1"
/db_xref="GeneID:30034095"
/translation="MNGSPFKGFYATKADPTVLLDYAHIDRDDETSILFVDGLVAHGH
RMFGLPPTEPTYFCKWDFDIGDVLIAAPLEVAEAFGRIGRCLVYSYEDMENSLLIPEP
PLYDVTFVSAKISKVKAQFSSEKYILELVTSAIDFRFNDLANERYSERVTLEIPSICT
SILDRFPYANKAHSIKATFRTSLIVSNFGQKRNMELCREYQQDHIRLHDAPFHRCAFV
LDSKRRKSPEYSVAGVRAGPVPSIPILGVPPPITRDTMPILDPNLGSGDLHRHNLGSD
EAASSSASTASRFNESLSNSSTDISLSLSDDDESVRYRPGPGNHGHGLTRRPPYAVDT
QKDVANNPFLPARGWKLLEKISADLLTDDDFNLQPTSNYNKGEVASPMSVLDPSTKYE
NLVIQFGSVEGDVSPFALGAINEIIESMGKEDMETSMDTLQIEFFKRLHMRHVTVLNP
EVTNIRLIVPNISIKYGDLEIREESRNVLNLENHIYFDFHGLNLVVRLREQSSVDQML
QAQHSPIKHTVYLNLDNSTIGVRKGGETASNIFEGARENSPVVLQIDRTEFWWHEDQE
TTGSLRIKAVDSFVMSEQVRWVAEFLDNNIEELKRINVSSTNESNRPKIKKQQQDHNR
TAYVLYQLAKAGESFNIQRDPSVLTRPAYIIQSPMHIRASTSWKIIMRLRHILRTVPS
EWREYHTEFLLNGSFETPPDIKEQTINIFARWRNWEMTNISSSYVFGYVFMETSLKER
LMSVATSTSLDIESIGLRLQYSESEDYLCFDYLKLLFNWSGKQYNEGPDSISPHDSVE
EDNNRLLDFESVLSCLHIRSRYSYHLLEVYDSVSDMIFKKNPQEVVKTESTESTAAVE
SQAKNTPGVLFPMVAMRTSFLLDQLSSVFILPSAELDLQGHQLKLTAFFEQLDPANRI
DAAVNVITNCSSFAIALNKRTPLYGTKLSQEKMLQILVNSSVLTYTSYGPMTTCSKYI
AARSTEFNVSMEKDLDQQLDFASQLLRHDWRCLREHVKACQNSSEAPIPKKTDPALQY
SGNLYIQLNAVHTKLTLRLLPSLSTIYQAENAFIKANHSEKEDFGVAELSIEEQLFRA
GSNLADLEAGFELFAFKMSPAVVLIRKEELKGMKDHSLEVLGSVSELSFNTESVAQLL
KLAQSGIWQDEAKALETHINNFKKEVCAVTMLGEPPATETVASQKSVKSLKPPLIRLS
MTFGKTSMLIPAYGYNAVMEVDGFGIQASSFIRFEDGFAEGFVTRTPLFITTSMDEFK
VLIYRKSSEQEKFVFIRLQTAMQLRLSQDGGKKIKHLLSLKSNFTRISFTPVIIQALV
EVVSSFSEDMSKYSVHEKGANPATSEIGEPKISDELGRGFVDKFCRNCTASMLVNDVS
VAWLFEDRGPTRFTSHPGLLFGYEVLDIQVVSFASHMVLKNVYLAPASERQIFLTKPV
TSRTANSALLPNVELRAEFEHEVSLQLAGESLKLTLLPNCVVLVSMFLDSISAAQALI
EQSALSADISTVPETPAPVKPLRFPFSWRLNLKFDGAVIELWDEGEFFDTGKVSKPTV
KKNSMYSEEGDTTGPEDEDDFRIPSLSLQTPALKAIFEYNSEGNKGSKDRFGCEISIT
PTSNVIYSRVVPVVVSMTRLVKEVVRANHQFNTARKREKNLLVNKTVRKHSAAGNGPT
TEDVELLFSAIDANIMIRFESQELTFSCLPTAKVSATVSVDRWFIGVHSGEVAKQGRI
VTAAFSIQNFKASLQHQYSRDVSGYIALEDAMIMLTKGKENTPLESIRVAGKISSIIF
DLNMKQMQDLEVFQNIWMPSGTLNTQQVREPRMEIASDPDYGMVMKFRRANTTVAIPW
SIDFTIIRIQGYIDMGQSIGKFDCKLDRFWISLQKSLDWEQHLSSGIDLIFLQSHGRL
DTTVSLEKPHLRTAIKWDKEVESFTVPLVQAVAGFSAFEVKISLEYHVFAMVYINSVH
FAMSNQRDHNKILGDRLIAVGELGTVNFFFTALAASYFVDVVYTFKRLRKASHASYEA
ILNDSSDKQQTHTNTPKTKRHDRKKLDDEALSNLFSKLRTMFDLRINSLNLYIYPGSF
TDSTLFTASLNAAHARFIQEQKDGTLESDLRLNMKSVLVALATSKAAPDLDIGLITVP
EFVSFAGQSVRGSTIIFVPVFHVSMRSWQKFGQPVVEYTFTSSIGGRVDIGWNLGSVT
FIREMWENHARALAQRQKTYSMTISDSKVIATGADLDKNLKEVELNPVFKYIAREPPD
IATPQLRQMGEATPPLEWLGLHRQKFPGLTHQFVIVGLQSLVHNIGKYFVHYEIALLE
LNEN"
gene complement(<675671..>678307)
/gene="CSF1"
/locus_tag="AWJ20_2210"
/db_xref="GeneID:30034097"
mRNA complement(<675671..>678307)
/gene="CSF1"
/locus_tag="AWJ20_2210"
/product="Csf1p"
/transcript_id="XM_018879139.1"
/db_xref="GeneID:30034097"
CDS complement(675671..678307)
/gene="CSF1"
/locus_tag="AWJ20_2210"
/note="Protein required for fermentation at low
temperature; plays a role in the maturation of secretory
proteins; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies;
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0006113 -
fermentation [Evidence IMP] [PMID 10781556]; GO_process:
GO:0051604 - protein maturation [Evidence IMP] [PMID
19433630]"
/codon_start=1
/product="Csf1p"
/protein_id="XP_018737082.1"
/db_xref="GeneID:30034097"
/translation="MRVPLVHGVSVACWRRGRRPRRARRSGVAFGGVEMSSVSAQFTT
VSILNNHEFSWVFIVDWILALVLTIFMIFYFNRAVGWLVSFLARWYLWRAHHAQLEVQ
SLQVSLLGGRIFFKNLSYVGTNEAICVLEGSFTWRYWLFYTRESLISGFLKDSHKGGS
DCEPQDFLGATSDDGAGSGVSGTTATGASSPPNPSPARSSKNRKYPARLVFDVTGVEW
FVFNRTPAYDALLDLAKKGSAVNSDQQEQEKQNQGDLDTNHKQNLQNLQKECSGAENT
SGTTGPDSLGTVRSSSSASTSSSSSDQSNLTSYDNMMLRMLPLEIRCHNKGAMVIGNK
DTSSLLIFHFESGVCSVDAAPPTSDLDKFKVIYKINTVKPILEMRPNVDYKGPDTVGF
PSVSPLSILKSMSWQSVLAHLSRLRRSARKKSKKYKKKSKASANKIRGKSKNRTNPRV
GRTTSRKTSFADGFGRFMTHRASTIYQTSSSSSSSEQDEEDDYYYDENYHPDENDDND
NDKEKPWRGLARYNIYYGNDNRDVSDDIPLDQLVDEYAKYSVILDAAEGTIAYHYDIP
GKVPDGIVPSTDDPLGNGRLENSPGRDPEWGVDMSFTSCTIHYGPWADRQRVFIQNML
FPRSFTNSTPEPVRMKGQDRLGMEFKLFIEFKEDVIIRIPTREASKNQKFYASYKENP
TANSLRAFGWLELKARHLSTMSYTGALVANKQGWNNNFRMDLKAPELRTSVNHGLVFS
ADRHTIQSTMKSPLQWNGIQEWDFRFNSEKVETFFLREHVSLLTDLITDFASGPPVTY
NQFVPFRYTIQWHISDYGIYLNVNDKNIINNPSDFDDNTFVSFQGQSVDIDILIPMEQ
VSSIKNRVDFKIAVSDNSVSKG"
gene complement(<679109..>682111)
/gene="SAC7"
/locus_tag="AWJ20_2211"
/db_xref="GeneID:30034098"
mRNA complement(<679109..>682111)
/gene="SAC7"
/locus_tag="AWJ20_2211"
/product="Sac7p"
/transcript_id="XM_018879140.1"
/db_xref="GeneID:30034098"
CDS complement(679109..682111)
/gene="SAC7"
/locus_tag="AWJ20_2211"
/note="GTPase activating protein (GAP) for Rho1p;
regulator of a Tor2p-mediated, Rho1p GTPase switch that
controls organization of the actin cytoskeleton; negative
regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and
membrane fluidity homeostasis signaling pathways;
potential Cdc28p substrate; SAC7 has a paralog, BAG7, that
arose from the whole genome duplication; GO_component:
GO:0005938 - cell cortex [Evidence IDA] [PMID 23878277];
GO_component: GO:0005737 - cytoplasm [Evidence IEA];
GO_component: GO:0005856 - cytoskeleton [Evidence
IEA,IEA]; GO_component: GO:0005622 - intracellular
[Evidence IEA]; GO_function: GO:0005096 - GTPase activator
activity [Evidence IEA]; GO_function: GO:0005100 - Rho
GTPase activator activity [Evidence IDA] [PMID 9038344];
GO_process: GO:0035024 - negative regulation of Rho
protein signal transduction [Evidence IGI] [PMID 9038344];
GO_process: GO:0090038 - negative regulation of protein
kinase C signaling [Evidence IMP] [PMID 10625705];
GO_process: GO:0090038 - negative regulation of protein
kinase C signaling [Evidence IGI] [PMID 11713681];
GO_process: GO:0090038 - negative regulation of protein
kinase C signaling [Evidence IMP] [PMID 12207708];
GO_process: GO:0032321 - positive regulation of Rho GTPase
activity [Evidence IDA] [PMID 9038344]; GO_process:
GO:0032956 - regulation of actin cytoskeleton organization
[Evidence IGI] [PMID 2183030]; GO_process: GO:0032956 -
regulation of actin cytoskeleton organization [Evidence
IGI] [PMID 9038344]; GO_process: GO:0060237 - regulation
of fungal-type cell wall organization [Evidence IGI,IMP]
[PMID 10625705]; GO_process: GO:0060237 - regulation of
fungal-type cell wall organization [Evidence IGI] [PMID
12949174]; GO_process: GO:0007165 - signal transduction
[Evidence IEA]"
/codon_start=1
/product="Sac7p"
/protein_id="XP_018737083.1"
/db_xref="GeneID:30034098"
/translation="MASAASPPSKSSLATWWKQFKQRPHLKDDELDYLSDSEGASGSG
SASAEGGASGGIYVSGRPKLRHLSHSSSAIGTIRSPSNQSVSVNGSGGGASGYGSASS
GIGGGSGYNSAVGTNAMSPETFQADSGASDNAIFGVPLKQSVDYAWVPISLTDPETGE
SFKYGHIPVIVAKCGRYLKKNATSVEGIFRLSGSARRIKELQVIFSTPTRYGKDLDWT
GFNVHDAANVLRRYLNNLPEPIVPLQFYEQFRDPLREYPRVVEYLTQQSAATSAALGA
GASGLSSLPSYRREQAQAQAFAQAQAQAQAAAGNQGGDDTSNHGPTIPLSASVPRSIT
LGLSNEESTSRSRTGSGSSIGAGTTGSIGTGQATASSTTLSASPGQSPFLPARTSSAQ
QQLQSTQQNGHTSTQPSQASQTTPEIGSILHSDINAAVDRYQHLISILPPLNRQLLMY
ILDLLSVFAARAEENLMPAANLAAIFQPSILSHPNHDMSPQEYHLSRSAIEFLVQHSN
KFLTHVENEAIRQHQQDKRDGKTTDLADPRAASSTAGAPGSTGLEPVNINSATESTPE
STTTSGNGSVSRTSGTTTTFVTPNPYSKQTYENGSGGSSGGGLTVHRRRHSKSLSSVG
SSGPPVPIVGSNYHSIQRVPVASISQEPNTTTAAAGGAPPPLQYQRQPSDDSNGFLTT
IKRTVSITRRPSQRRSTSGSSTSSAARLRSSSGAKPTPGTLGGSVVSTPTAQSPAPQA
VNIPEIITTNASQVDITTPSPGFEAVSLQNTPGASPPLEPTRSPDSKKGLSNLFSRKS
KSPNRGRASSDDYTRPEPSNFSNQSIDQAMAMLSTTGTTDLGSGSSGVPGGMHHIQSS
LPPPQQPFLHHSNHSNSSLGQSSTTSGGGAGDSDYDTGNPRPQDDSGSPNRKTKSRWR
RSLMMFNMAGVGDSFHDLSNTSTTSSYGASHVPDLQTGDNLSPTNSAIRSQSPGRWFK
KKALNRGRSSSRDGNHPDPDAPSDTAPQ"
gene complement(<685524..>686900)
/gene="RVS167"
/locus_tag="AWJ20_2212"
/db_xref="GeneID:30034099"
mRNA complement(<685524..>686900)
/gene="RVS167"
/locus_tag="AWJ20_2212"
/product="amphiphysin"
/transcript_id="XM_018879141.1"
/db_xref="GeneID:30034099"
CDS complement(685524..686900)
/gene="RVS167"
/locus_tag="AWJ20_2212"
/inference="similar to AA sequence:KEGG_Orthology:K12562"
/note="Actin-associated protein with roles in endocytosis
and exocytosis; interacts with Rvs161p to regulate actin
cytoskeleton, endocytosis, and viability following
starvation or osmotic stress; recruited to bud tips by
Gyl1p and Gyp5p during polarized growth; homolog of
mammalian amphiphysin; GO_component: GO:0030479 - actin
cortical patch [Evidence IDA] [PMID 10393809];
GO_component: GO:0030479 - actin cortical patch [Evidence
IDA] [PMID 16239147]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 24390141]; GO_component: GO:0005856 -
cytoskeleton [Evidence IEA,IEA]; GO_component: GO:0043332
- mating projection tip [Evidence IDA] [PMID 10393809];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 19053807]; GO_function: GO:0003779 - actin
binding [Evidence IEA]; GO_function: GO:0008092 -
cytoskeletal protein binding [Evidence IPI,ISS] [PMID
10388809]; GO_function: GO:0008289 - lipid binding
[Evidence IDA] [PMID 20610658]; GO_process: GO:0051666 -
actin cortical patch localization [Evidence IMP] [PMID
8590801]; GO_process: GO:0006897 - endocytosis [Evidence
IMP] [PMID 8590801]; GO_process: GO:0060988 - lipid tube
assembly [Evidence IDA] [PMID 20610658]; GO_process:
GO:0016192 - vesicle-mediated transport [Evidence IGI,IPI]
[PMID 15802519]"
/codon_start=1
/product="amphiphysin"
/protein_id="XP_018737084.1"
/db_xref="GeneID:30034099"
/translation="MNIGTHTKDLVYIDAERRFKELEDETKKLHAESTKYSEAVNGML
KHQIEFSKAVEEIYKPISGRMSDPNSTRPEGNPEGIQACEQYRDVVFELQATLKPELE
MIETRIVAPADELLKVIKAIRKMSVKRDHKQLDLDRHTATLTKLQGKKERSIKDEKNM
YTAENNVEIAKQEYDYFNNLLKEELPKLFELEAEFIRPLFQSFYYMQLNIFYTLYNRM
EEMKIPYFDLTADIEESFNRKRGDIQEQAEAIGITHFRVGHAKSKLELTKKKYGKETE
AGAAGAAAGAGAYGAGAYGAGAAAAGSEGVPPPYQGYGQQPPQEKYVYQAPGAPAAAG
YGQQPAYGQPAAAPAGYGQQPAYGQPAATAAQPVYGQPAAAAAAAAVPTAYSPSPVSP
VAASPVAPVEYCTALYDYEAQAHGDLSIHAGDRIEIVQRTADPNGWWTGKLNGVQGVF
PGNYVQLG"
gene complement(<690120..>694215)
/gene="UTP10"
/locus_tag="AWJ20_2213"
/db_xref="GeneID:30034100"
mRNA complement(join(<690120..690135,690224..>694215))
/gene="UTP10"
/locus_tag="AWJ20_2213"
/product="Utp10p"
/transcript_id="XM_018879142.1"
/db_xref="GeneID:30034100"
CDS complement(join(690120..690135,690224..694215))
/gene="UTP10"
/locus_tag="AWJ20_2213"
/inference="similar to AA sequence:KEGG_Orthology:K14550"
/note="Nucleolar protein; component of the small subunit
(SSU) processome containing the U3 snoRNA that is involved
in processing of pre-18S rRNA; mutant has increased
aneuploidy tolerance; GO_component: GO:0030686 - 90S
preribosome [Evidence IDA] [PMID 12150911]; GO_component:
GO:0030686 - 90S preribosome [Evidence IDA] [PMID
17652137]; GO_component: GO:0016021 - integral component
of membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_component: GO:0005730 -
nucleolus [Evidence IEA]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 12068309]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 12150911];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
17652137]; GO_component: GO:0005730 - nucleolus [Evidence
IDA] [PMID 22842922]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_component: GO:0030688 - preribosome,
small subunit precursor [Evidence IDA] [PMID 17652137];
GO_component: GO:0033553 - rDNA heterochromatin [Evidence
IDA] [PMID 15489292]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0032040 - small-subunit processome [Evidence IDA] [PMID
12068309]; GO_component: GO:0034455 - t-UTP complex
[Evidence IDA] [PMID 17515605]; GO_function: GO:0030515 -
snoRNA binding [Evidence IPI] [PMID 12068309]; GO_process:
GO:0000480 - endonucleolytic cleavage in 5'-ETS of
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 17652137]; GO_process:
GO:0000447 - endonucleolytic cleavage in ITS1 to separate
SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic
rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 17652137]; GO_process: GO:0000472 -
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence
IMP] [PMID 17652137]; GO_process: GO:0000462 - maturation
of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID 12068309];
GO_process: GO:0045943 - positive regulation of
transcription from RNA polymerase I promoter [Evidence
IMP] [PMID 15489292]; GO_process: GO:0006364 - rRNA
processing [Evidence IEA]; GO_process: GO:0042254 -
ribosome biogenesis [Evidence IEA]; GO_process: GO:0006351
- transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Utp10p"
/protein_id="XP_018737085.1"
/db_xref="GeneID:30034100"
/translation="MISLKDSGANDQEIVGRFLPYCSNAISLKKDPDAQTAAYIILAV
LGSQFRLNKEVKSAAIQSIILNWSRASVKSGFSCITQLVKLDDTLEPFEKEDWDAIEK
IRAIDTEINALSEQYDISQFIVRWALSVLKYSPEKIYLVQSVLASGKFGISHGLDIVL
RASIKLATQASDANVKEQLVSLFEFVLSSEEGAKGFKEVLANAGVSFDQLELGVQSSI
QTKFAFVRGQEESAAVDDDVEMTQAEELTATVTLTEELKSRSPVKVASFLSLDTNKEF
RELVNLFTLGLQSSQSDILKSLQIDAESQPSFLVRIWTGAYANLARVRALELFSAIVE
ENPSVDYQAVIPFLLVALSDESERVRRGASSIIRSLATRYPIKKPTVWALDNLYGEGD
QSSEVKWLTSTNIKVLLNDVLLPNIDECYLDADYISKRIGEVASDKEFKSLQEPLLTF
LTSHVLSMKLPQMKVSLLQILRKWAKSSSTSKLLTPLLTSWIDERDTWVSICEEERVS
FDKLETAVVSIVSSGEKSGIAFLEQAIRSSSNLSDKAGQRLVYLWKSAALKPDTQLGL
LRFLSDVAVDESITYDSLEVLNNIEITTPHFVSLLDDCKLERDSAAANSSGSRSGTAS
PAVPKRRRRSSNSSREKFRGSELISFAEKHLRKLTVILELLDKNKSEGPSCMTLLSQL
FTILGEILSLGADSNLPSLYTEQLLANCMISAVEQLKHQRHTKIDSALVRVDIIVSCI
RSSSSPQVQNRFLLLVASLAELAPEVVLHSVMPIFTFMGANTVRQDDEFSAHVIQQTI
SQVIPALVSNNNGTSNEEIEFLLLSFVAAFAHIPRHRRVKLFGALVRTLKPEASLHKL
LILFGNKYSEAKIKRKSADLKSYVQFGTTFVRSFTVVDQVTAIRNFLAVSESIPLTEG
ETPSEGGQLFTALTNGNGTNVTDNATLKANVYEFLTNVFGDDDIVGGYPSLRAQILKE
EADKAVVMDICSEIIEQLLKTRKATSDNTELSAVPSLAENIFNFLGKMLEVLPMGSFV
EVVESLLASSDIDVKVHLLALVQSKFDVELSTDEDAVKAAINCTALLKNYLSSTEDAH
IIQLVLDDLDVIIAKFGSQMSPEELLAALTAIVGERGLLNSDIKVFVAAVAASSSIST
VLGARMIGLFGKIVPVVFKRFETSLALENKDDAKLIQLATFGLVSGLVKRIPSFMTSS
VKKVFEFVLVSNATTVDVRTRVMDVIRNNIEDKQVISALTGTWKYAVDNGMDSINIFL
DSLDKVIDVSPKKVVSSQSSIIIGFLLQAFEVRGQGNYGKEDTNRIEAKAVSSAIKVI
MKLNDKTFQPFYLEA"
gene <696342..>697679
/locus_tag="AWJ20_2214"
/db_xref="GeneID:30034101"
mRNA <696342..>697679
/locus_tag="AWJ20_2214"
/product="hypothetical protein"
/transcript_id="XM_018879143.1"
/db_xref="GeneID:30034101"
CDS 696342..697679
/locus_tag="AWJ20_2214"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737086.1"
/db_xref="GeneID:30034101"
/translation="MPDLSESSEFMRMQTESARPGSSSQAGHVPGTYNKSLKSIASNN
GNGSSTAVDSVGGFEGLEHRQIRSNDIELESAPRYASESDPYSLSSGLKTKSELKRIR
SNEDGKSRSVGRKLQQFYESQNEQIEMLLKPVEDHRAEAKQAEGDNQLKYRIAVYGSF
AANIILAALQIYGAASSGSLSLITTMADAIFDPMSNITLILANRAVNHVDSRRFPSGK
ARIETVGNITFCFLMSAVGAIIIVLSARDIAGGNSEETKKFHLPSVIAVAVAFCTKFA
LWLYCWALKDTYSQVNILWQDHRNDLFINGFGILTSVAGSKLRWWIDPMGAIILSVFI
DIVWLRTATQEFLLLIGQTADNSIQQLITYLAVTHSPEIDQVDTVRVYHSGPRLIAEV
DVVMNENMTLKETHDIAEALQFKLESLPDVERAYVHVDYETSHKPEHFLKKEL"
gene <698523..>701360
/gene="ATG11"
/locus_tag="AWJ20_2215"
/db_xref="GeneID:30034102"
mRNA <698523..>701360
/gene="ATG11"
/locus_tag="AWJ20_2215"
/product="autophagy protein ATG11"
/transcript_id="XM_018879144.1"
/db_xref="GeneID:30034102"
CDS 698523..701360
/gene="ATG11"
/locus_tag="AWJ20_2215"
/inference="similar to AA sequence:KEGG_Orthology:K08330"
/codon_start=1
/product="autophagy protein ATG11"
/protein_id="XP_018737087.1"
/db_xref="GeneID:30034102"
/translation="MTQLKLEAIADGQEVFLFDKSLISPGAKTYAENGLVGKEDRGPP
LALPPRPAVNTTSSLSDRFMTHSDWTQKCLLVGKDCGNRVNELHMSINIIKRGLKTSL
EHMMRHSQGLKKSFDSKVDFAVDLDQNMQATGEWEVYLEKVKKIELLPAFREQGEGRD
RLSSWIDESVIRKANKQCSEFDQKIQNNISHLVKVVNEVIERSTRLEQDAMAMASKDA
ESDVPAEATSRHVRELLQDLTALANKIQRDSESIGGVSEQTAVRIENLHKREFFPQIE
ATVNELIGIHEEWQKAKVKCQNLAIGYLSTLSHIQYDTSMVRPELQKLTMLLQTTEDL
RATVAQVIDLPFLYGALIIETVRRNHWTGYIRENVSQTAESLATKRLDEIKRRKKWKN
HYGKILPKVVDIINEDIAEIEVNFVSGEVNGDGDASSTDSDQVQAYIKLLTSLGLREA
AEELEQEHQSMLSQLESDIADKNLAKLETTKQSDHHTDTSSPAKVFKSGTLAEAYTDE
NAALTAKLKGYESRIRILEDLLHKQQFRGESTRSEANRRSASRDKSHSPGSPRMIERL
HFLETQKSNDSKIIAQLEQEKRELAESLSAKELALKDVMTVKSDLLANMLSQETEFIQ
ERKAHHEEVNELKSRIDELELDLDRADEKLQLENDQLREEVDSYKEKIEQLEQENSNL
KQQEEKLHKKIEYYDTRTRDLSQRLFTGFKRSCEVLESMGLQVTKEMDGNLITFKVNR
VRGLGRRSSAARSEILEQSLEENGNGNMVSNPTLNEATPEDLRVLYWMDGEPDEERYR
EFINEIYIDYDIFRDSACKRLRDVEHLARKLQKESKHLRERKTQIEEELQSRLSLRSF
KVGDLALFLPTRDPTRVPNPWAAFNVNAPHYFLKQEDSHQLSNREYLVGRITHIEERV
VNKSLDNDEDNPFDLSDGLRWYLLDAVPAEW"
gene <703797..>704744
/gene="KES1"
/locus_tag="AWJ20_2216"
/db_xref="GeneID:30034103"
mRNA <703797..>704744
/gene="KES1"
/locus_tag="AWJ20_2216"
/product="oxysterol-binding protein KES1"
/transcript_id="XM_018879145.1"
/db_xref="GeneID:30034103"
CDS 703797..704744
/gene="KES1"
/locus_tag="AWJ20_2216"
/note="One of seven members of the yeast oxysterol binding
protein family; involved in negative regulation of
Sec14p-dependent Golgi complex secretory functions,
peripheral membrane protein that localizes to the Golgi
complex; KES1 has a paralog, HES1, that arose from the
whole genome duplication; GO_component: GO:0000139 - Golgi
membrane [Evidence IDA] [PMID 11916983]; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
8978672]; GO_component: GO:0019898 - extrinsic component
of membrane [Evidence IDA] [PMID 11916983]; GO_function:
GO:0008289 - lipid binding [Evidence IDA,IMP] [PMID
20008566]; GO_function: GO:0008142 - oxysterol binding
[Evidence ISS] [PMID 11238399]; GO_function: GO:0008142 -
oxysterol binding [Evidence IMP,IPI] [PMID 16136145];
GO_function: GO:0070300 - phosphatidic acid binding
[Evidence IDA] [PMID 20729555]; GO_function: GO:0005546 -
phosphatidylinositol-4,5-bisphosphate binding [Evidence
IMP,IPI] [PMID 11916983]; GO_function: GO:0070273 -
phosphatidylinositol-4-phosphate binding [Evidence IDA]
[PMID 20729555]; GO_function: GO:0015248 - sterol
transporter activity [Evidence IDA,IMP] [PMID 20008566];
GO_process: GO:0035621 - ER to Golgi ceramide transport
[Evidence IMP] [PMID 24213531]; GO_process: GO:0006897 -
endocytosis [Evidence IGI] [PMID 15173322]; GO_process:
GO:0006887 - exocytosis [Evidence IGI] [PMID 17004323];
GO_process: GO:0006629 - lipid metabolic process [Evidence
IEA]; GO_process: GO:0030011 - maintenance of cell
polarity [Evidence IGI] [PMID 17004323]; GO_process:
GO:0010922 - positive regulation of phosphatase activity
[Evidence IDA] [PMID 21295699]; GO_process: GO:0006892 -
post-Golgi vesicle-mediated transport [Evidence IGI] [PMID
11916983]; GO_process: GO:0006892 - post-Golgi
vesicle-mediated transport [Evidence IGI] [PMID 8978672];
GO_process: GO:0006694 - steroid biosynthetic process
[Evidence IEA]; GO_process: GO:0008202 - steroid metabolic
process [Evidence IEA]; GO_process: GO:0016126 - sterol
biosynthetic process [Evidence IEA]; GO_process:
GO:0015918 - sterol transport [Evidence IDA,IGI] [PMID
16585271]; GO_process: GO:0015918 - sterol transport
[Evidence IDA,IMP] [PMID 20008566]"
/codon_start=1
/product="oxysterol-binding protein KES1"
/protein_id="XP_018737088.1"
/db_xref="GeneID:30034103"
/translation="MVLVLKWFIGTLRGQYCSRNEKLGSEKKPLNPFLGELFVGKWAG
DAESGETVLASEQVSHHPPITGYSIWNDKHGVYLNGYNGMKARIATTTISVKQNGHAT
YYLKAFDETYIISLPALHIEGILFGAPYVELEGKSFIHGSSGYSVSIEYTGKGYFSGK
KNSFKAKIYKGSEDAALYRVSGQWSESSKIKDEKTGVETPFLDSLKITSESLQVKPEA
EQSELESRRAWSKVAAAIREGDYELIHQEKSKIENDQRELRKTEQAEGKTWPSKWFEE
VPVSNAPEVYLTLSKAAGVEPETTWTFVRSKFDAADVKP"
gene <708842..>709147
/gene="NHP6B"
/locus_tag="AWJ20_2217"
/db_xref="GeneID:30034104"
mRNA <708842..>709147
/gene="NHP6B"
/locus_tag="AWJ20_2217"
/product="Nhp6bp"
/transcript_id="XM_018879146.1"
/db_xref="GeneID:30034104"
CDS 708842..709147
/gene="NHP6B"
/locus_tag="AWJ20_2217"
/note="High-mobility group (HMG) protein; binds to and
remodels nucleosomes; involved in recruiting FACT and
other chromatin remodelling complexes to the chromosomes;
functionally redundant with Nhp6Ap; required for
transcriptional initiation fidelity of some tRNA genes;
homologous to mammalian HMGB1 and HMGB2; NHP6B has a
paralog, NHP6A, that arose from the whole genome
duplication; GO_component: GO:0005694 - chromosome
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IEA,IEA]; GO_component: GO:0005634 - nucleus
[Evidence IDA] [PMID 3169249]; GO_function: GO:0003677 -
DNA binding [Evidence IEA]; GO_function: GO:0008301 - DNA
binding, bending [Evidence IDA] [PMID 7721780];
GO_function: GO:0031491 - nucleosome binding [Evidence
ISS] [PMID 11432837]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IDA] [PMID
19158363]; GO_process: GO:0006281 - DNA repair [Evidence
IEA]; GO_process: GO:0070898 - RNA polymerase III
transcriptional preinitiation complex assembly [Evidence
IGI] [PMID 11287614]; GO_process: GO:0070898 - RNA
polymerase III transcriptional preinitiation complex
assembly [Evidence IDA] [PMID 17178828]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEA]; GO_process: GO:0006338 - chromatin
remodeling [Evidence IGI] [PMID 14739928]; GO_process:
GO:0001195 - maintenance of transcriptional fidelity
during DNA-templated transcription elongation from RNA
polymerase III promoter [Evidence IDA] [PMID 16407207];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IEA]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IGI] [PMID 8946917]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Nhp6bp"
/protein_id="XP_018737089.1"
/db_xref="GeneID:30034104"
/translation="MPKEPGRRRTKEEIRKKKKDPNAPKRALSAYMYFANENRDNIKN
ENPDISFGQIGKVLGEQWKALTDAEKVPYEAKATQDKKRYEEEKAAYQASKAQEYSE"
gene complement(<709876..>712212)
/gene="UBP16"
/locus_tag="AWJ20_2218"
/db_xref="GeneID:30034105"
mRNA complement(<709876..>712212)
/gene="UBP16"
/locus_tag="AWJ20_2218"
/product="Ubp16p"
/transcript_id="XM_018879147.1"
/db_xref="GeneID:30034105"
CDS complement(709876..712212)
/gene="UBP16"
/locus_tag="AWJ20_2218"
/inference="similar to AA sequence:KEGG_Orthology:K11874"
/note="Deubiquitinating enzyme anchored to the outer
mitochondrial membrane; probably not important for general
mitochondrial functioning, but may perform a more
specialized function at mitochondria; GO_component:
GO:0005737 - cytoplasm [Evidence IC] [PMID 8982460];
GO_component: GO:0005741 - mitochondrial outer membrane
[Evidence IDA] [PMID 12914939]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
16407407]; GO_function: GO:0008234 - cysteine-type
peptidase activity [Evidence IEA]; GO_function: GO:0016787
- hydrolase activity [Evidence IEA]; GO_function:
GO:0008233 - peptidase activity [Evidence IEA];
GO_function: GO:0004843 - ubiquitin-specific protease
activity [Evidence TAS] [PMID 8982460]; GO_process:
GO:0016579 - protein deubiquitination [Evidence TAS] [PMID
8982460]; GO_process: GO:0006508 - proteolysis [Evidence
IEA]; GO_process: GO:0006511 - ubiquitin-dependent protein
catabolic process [Evidence IEA]"
/codon_start=1
/product="Ubp16p"
/protein_id="XP_018737090.1"
/db_xref="GeneID:30034105"
/translation="MNVLRRKKKSGKYTVGLTNRANDCFANSNIQALAAVPGLVDYLL
QLNAAAIALSNPDAVRMSVPGTSVPGNTTAEKRKLASSTEKSLPQTPDSQPAVAIADS
PAAGGTATPASSAKTNTNNSGATTDIPTRPTTPSAQIATAVTPKSPSGGYGASMPASN
AQTLTPLLSNALLRIIKELNEPILVPKTLSPWTFLGVLEKIYKSRISRNQHDAHELLH
LILETLENEYDKIVKESRSRTEKQSIWGNIAASHPSPLEDAELESEQEAPKSFAFSGS
TIDRITCSRCGYSPPATPSAFLVLSLMVPQKKSAQLTDLLDGLSSPEYIQDYGCQLCR
LKHASMMKNGDQFKQYLSDPSKLPSELEDKLPKNIVSPIAKSTRFHRLPDVLAIHLSR
SIYGGFGASRNSCKVSTKEFIELTENETGNIIRYKLMAMIRHKGTHQMGHYECFRRKN
LDTWRDILQMLDEDEKDREIKKGLRLHKQQTPSVSSFQGSGTGTGTAQSATPTPRQLA
TTASTAGTISANSSPRLDLATGAINGNYGGTAAVPTATATPVASSPTIASMNSSNSSS
GASSSITTKSSATEITNYTMSTTPSSPLTTITTPSSSIPETAFSSSATTGDSQSPAPS
LASDNVTLSSASSSISGVSAASAPLRLTADTPSSPALSPPPSAIPLPYPADRSMPSPA
SVSISEFASAEISLDAIRAVPLSAATLNGTKRPVFTAPTTPTRSPTPTLLSVQQNVSP
PSHHEWWKVSDDKVWECTTKEVLREESGAYLLFYERLT"
gene <713753..>714229
/locus_tag="AWJ20_2219"
/db_xref="GeneID:30034106"
mRNA <713753..>714229
/locus_tag="AWJ20_2219"
/product="putative heme/steroid binding protein"
/transcript_id="XM_018879148.1"
/db_xref="GeneID:30034106"
CDS 713753..714229
/locus_tag="AWJ20_2219"
/note="potential heme/steroid binding protein; similar to
A. thaliana F17I14_130; allele of CaO19.4522"
/codon_start=1
/product="putative heme/steroid binding protein"
/protein_id="XP_018737091.1"
/db_xref="GeneID:30034106"
/translation="MSEQPQLGPPPDRKKMLAPPPGNAQSTLFPSINSAQRASGSRAI
SGPQPAAAARRKKVALQPGHSALDWANLRNSGANLRVGTCSPPAAGAAPQTPLLLSLR
SSRVSGVPFKGSRGLSDASGDDSSGARGATGSGAEPQPTEAHSPVPYSDTNRFSGS"
gene <717982..>720456
/locus_tag="AWJ20_2220"
/db_xref="GeneID:30034108"
mRNA <717982..>720456
/locus_tag="AWJ20_2220"
/product="RNAPII degradation factor (predicted)"
/transcript_id="XM_018879150.1"
/db_xref="GeneID:30034108"
CDS 717982..720456
/locus_tag="AWJ20_2220"
/codon_start=1
/product="RNAPII degradation factor (predicted)"
/protein_id="XP_018737092.1"
/db_xref="GeneID:30034108"
/translation="MSSKKYTSALTTLRELFPDWKDDDLLAVLEETSGDIEQSVSLIA
EGHVSQWGQVKKKTRESTRGKSKGPANADGKESNTTSAHRHNGSAASHGHGSSTHGSN
NTGTGAPRGQRSERGRSRAPKRVNGSTHTHTNANSTTSSAAATANNNNTSDATSTPAA
WETDAAGVPAGSAVAAASIAASNASSTSTTDSTAATTNSSTSESAPSAPKTRSWADAL
SKKSTTAPVAAKRQSQPQQHQQQERAAQKPQPSASADIDNTTTTTTTTSSSLPESNTA
ATSTPAAAATAASTETTAPVSNSAPTAVPALPVAPQKLSWAAIAKPKEEKKPAPAAPV
ASTATTNASPASAAASETPVAAATSTETTTQPEQTEAFTQEPAAETVSEPIPVSIPPG
LESKPEPAVPAATSNTASTGAAAAAAPRRVTQEVPVILPGSVSRTVDRVGVQFGSLDL
NEQTPATESASQTAQPAQQQAQAQQQQQQQQIASETANSSATSTAPAGIPEQGQTAGA
AAAAQPAYYGYGQTQAQTAGSVQQKPYENAYSQNHYVYPGTHPGSHPGFATGYSVPTD
YQSPYGAGVSDQRNSYNNYYYQQQAAAAGQQQPGLTGNQQQTQSQHNGSLDSSRYGTS
AGATGAEAGANSAVAGGAGVAQSSNANSPASVAAGVYQQPSMNPYYMSPAYAAYYYQT
YGYNQQAALAAQYGSPAGPQKGFFNQQQSYYDYRQPGVGGQVQGQQGQSQQQAQGQQQ
GQSQGQQQQQSQAQGQTSQAGGLTGISDFLQREDTSKQTPGSPQQHQAGLPQQPQYPV
QQNLYSYAGYGQYSGQQGQRQGWGYN"
gene complement(<720792..>722135)
/gene="CPA1"
/locus_tag="AWJ20_2221"
/db_xref="GeneID:30034109"
mRNA complement(<720792..>722135)
/gene="CPA1"
/locus_tag="AWJ20_2221"
/product="carbamoyl-phosphate synthase
(glutamine-hydrolyzing) CPA1"
/transcript_id="XM_018879151.1"
/db_xref="GeneID:30034109"
CDS complement(720792..722135)
/gene="CPA1"
/locus_tag="AWJ20_2221"
/inference="similar to AA sequence:KEGG_Orthology:K01956"
/note="Small subunit of carbamoyl phosphate synthetase;
carbamoyl phosphate synthetase catalyzes a step in the
synthesis of citrulline, an arginine precursor;
translationally regulated by an attenuator peptide encoded
by YOR302W within the CPA1 mRNA 5'-leader; GO_component:
GO:0005951 - carbamoyl-phosphate synthase complex
[Evidence IDA] [PMID 206535]; GO_component: GO:0005951 -
carbamoyl-phosphate synthase complex [Evidence ISS] [PMID
6086650]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 22842922]; GO_function: GO:0005524 - ATP
binding [Evidence IEA]; GO_function: GO:0004088 -
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [Evidence IEA]; GO_function: GO:0004088 -
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [Evidence IMP] [PMID 6086650]; GO_function:
GO:0004088 - carbamoyl-phosphate synthase
(glutamine-hydrolyzing) activity [Evidence IDA] [PMID
8626695]; GO_function: GO:0016874 - ligase activity
[Evidence IEA]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006526 - arginine
biosynthetic process [Evidence IEA,IEA]; GO_process:
GO:0006526 - arginine biosynthetic process [Evidence IMP]
[PMID 9290206]; GO_process: GO:0070409 - carbamoyl
phosphate biosynthetic process [Evidence IEA]; GO_process:
GO:0008652 - cellular amino acid biosynthetic process
[Evidence IEA]; GO_process: GO:0006543 - glutamine
catabolic process [Evidence IEA]"
/codon_start=1
/product="carbamoyl-phosphate synthase
(glutamine-hydrolyzing) CPA1"
/protein_id="XP_018737093.1"
/db_xref="GeneID:30034109"
/translation="MLRTLSKSVSAGLKLNASPLARLSSKSYSTVNTALFNSRSDLDR
ATFTIRDGPVFHGKSFGANTNISGEAVFSTGMVGYPESMTDPSYRGQILVFTQPLIGN
YGVPSSTARDEFGLLKYFESPHCQCIGVVVADAAMQYSHWTAVESLGDWCKREGVAAI
TGVDTRSIVTYLREQGSSLARITVGEEYDADEDEAFMDPGAINLVHKVSTKQPFHVPA
ATIPGQENIHVAVIDCGVKENILRSLVSRGASITVFPYDYPIHKISHHFDGIFISNGP
GDPTHCSSTVYNLKKTMETYDGPIFGICLGHQLLALAAGGKTIKLKYGNRAHNIPALD
LMTGKCHITSQNHGYAVDVATLPSEFKPYFINLNDQSNEGMIHESRPIFSTQFHPEAK
GGPLDSAFLFDKYLADIQAFKQKKSAQGLVNAAPSPLFVDILPKERVGVEINPQA"
gene complement(<724509..>725435)
/gene="DUS4"
/locus_tag="AWJ20_2222"
/db_xref="GeneID:30034110"
mRNA complement(<724509..>725435)
/gene="DUS4"
/locus_tag="AWJ20_2222"
/product="Dus4p"
/transcript_id="XM_018879152.1"
/db_xref="GeneID:30034110"
CDS complement(724509..725435)
/gene="DUS4"
/locus_tag="AWJ20_2222"
/inference="similar to AA sequence:KEGG_Orthology:K05545"
/note="Dihydrouridine synthase; member of a widespread
family of conserved proteins including Smm1p, Dus1p, and
Dus3p; GO_component: GO:0005575 - cellular_component
[Evidence ND]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0050660 - flavin
adenine dinucleotide binding [Evidence IEA]; GO_function:
GO:0016491 - oxidoreductase activity [Evidence IEA];
GO_function: GO:0017150 - tRNA dihydrouridine synthase
activity [Evidence IEA]; GO_function: GO:0017150 - tRNA
dihydrouridine synthase activity [Evidence ISS] [PMID
12003496]; GO_function: GO:0017150 - tRNA dihydrouridine
synthase activity [Evidence IMP] [PMID 14970222];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]; GO_process: GO:0002943 - tRNA
dihydrouridine synthesis [Evidence IEA]; GO_process:
GO:0006400 - tRNA modification [Evidence ISS] [PMID
12003496]; GO_process: GO:0006400 - tRNA modification
[Evidence IMP] [PMID 14970222]; GO_process: GO:0008033 -
tRNA processing [Evidence IEA,IEA]"
/codon_start=1
/product="Dus4p"
/protein_id="XP_018737094.1"
/db_xref="GeneID:30034110"
/translation="MMLAREFVRNQNARITDFSTNEADTPLIVQFGANNEVDLARAAK
MIMPYTDGIGLNCGCPIKEQVREGIGAALMTDPERVASMVRAVKATCGPEFCVEVKIR
IHSDLQETVRFARLVEDAGADYITVHGRRKTQRSSEPANWEAIKLIKESVKCPVVANG
DAFTMDDVNKIVELTGVDGVMSARGILANPALFSGYNKTPWGAIELFWDYVTSYGLPF
RLTQHHFSEMVELEFTRKQKRSLNESNNLPELLDWFDARFDLKRRGEPGFGERVEFPW
KSTYHERELDNELNENASLNLASDLASVHLAS"
gene <726285..>727235
/locus_tag="AWJ20_2223"
/db_xref="GeneID:30034111"
mRNA <726285..>727235
/locus_tag="AWJ20_2223"
/product="hypothetical protein"
/transcript_id="XM_018879153.1"
/db_xref="GeneID:30034111"
CDS 726285..727235
/locus_tag="AWJ20_2223"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737095.1"
/db_xref="GeneID:30034111"
/translation="MPSLNNGSIIGSIFGKGTVYESLDNSEDDNLYNLQLHIDDNQTT
TPLGGGVGIDPRAQDPRHDSEYNTSGNGGLAIGSVTETGRSGRRDSARNQPLMNSDDS
DESDDDDGPSDDILTERRNIYESAISSPPHVNHGTRLHPNYHHTDNVSDEVQEDGDNE
IPQSLMVEPTAKTTVSSGATLGSTANHGTVVKGGHGGSDISAINSNTTLHQMSSPKQA
RRIFSANDLGDQPSARFGSPNHTHRRSPLAMASGHGPPSSVLPLYKTDLKGNNLSASS
WSAAKSTHNLMIDPKERAKWKWANVENLDIFLQEVSTGSP"
gene <727652..>728827
/gene="ATG9"
/locus_tag="AWJ20_2224"
/db_xref="GeneID:30034112"
mRNA <727652..>728827
/gene="ATG9"
/locus_tag="AWJ20_2224"
/product="autophagy protein ATG9"
/transcript_id="XM_018879154.1"
/db_xref="GeneID:30034112"
CDS 727652..728827
/gene="ATG9"
/locus_tag="AWJ20_2224"
/inference="similar to AA sequence:KEGG_Orthology:K17907"
/codon_start=1
/product="autophagy protein ATG9"
/protein_id="XP_018737096.1"
/db_xref="GeneID:30034112"
/translation="MQTISWKKVVEKIELLKNQNLGTSGKAATKGSAINSRQKLDKHS
IANRIMRKENYLVAMINKDILDLRVPLPFLRKKQFFTRTLEWNLSLCIMDYAFNEQGE
LRPIFLRESQRRLLSDGLRRRFLFAGIMNILCAPFIIAYLTLLYFFRYFNEYHRDPSA
IGTRQYTPLAEWKMRELNELSHLFQNRLNMSYPAASRYINQFPREKTVSVLRFVAFVC
GSFAAVLGIISLVDPELFLGFEITKDRTVLFYIGIFGSIVAISRSMIPDETLVFDPVT
SMMYVAEFTHYLPKEWEGRLHSDSVKAEFAAMYDLKIMIILNELASVIIAPFILWFSL
PNSCDRIIDFFREFSVHVDGLGYVCSFAMFNFDRVQKKNGVSIGYTHGGFYRLRQGD"
gene complement(<729549..>732047)
/gene="NOP14"
/locus_tag="AWJ20_2225"
/db_xref="GeneID:30034113"
mRNA complement(<729549..>732047)
/gene="NOP14"
/locus_tag="AWJ20_2225"
/product="Nop14p"
/transcript_id="XM_018879155.1"
/db_xref="GeneID:30034113"
CDS complement(729549..732047)
/gene="NOP14"
/locus_tag="AWJ20_2225"
/inference="similar to AA sequence:KEGG_Orthology:K14766"
/note="Nucleolar protein; forms a complex with Noc4p that
mediates maturation and nuclear export of 40S ribosomal
subunits; also present in the small subunit processome
complex, which is required for processing of pre-18S rRNA;
GO_component: GO:0030686 - 90S preribosome [Evidence IDA]
[PMID 12150911]; GO_component: GO:0030692 - Noc4p-Nop14p
complex [Evidence IPI] [PMID 12446671]; GO_component:
GO:0005739 - mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005730 - nucleolus
[Evidence IEA]; GO_component: GO:0005730 - nucleolus
[Evidence IDA] [PMID 10684247]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 12068309]; GO_component:
GO:0005730 - nucleolus [Evidence IDA] [PMID 12150911];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0032040 - small-subunit
processome [Evidence IEA]; GO_component: GO:0032040 -
small-subunit processome [Evidence IDA] [PMID 12068309];
GO_function: GO:0030515 - snoRNA binding [Evidence IPI]
[PMID 12068309]; GO_process: GO:0000480 - endonucleolytic
cleavage in 5'-ETS of tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
15489292]; GO_process: GO:0000447 - endonucleolytic
cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and
LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000472 - endonucleolytic cleavage to
generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [Evidence IMP] [PMID 15489292];
GO_process: GO:0000462 - maturation of SSU-rRNA from
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 12068309]; GO_process:
GO:0006364 - rRNA processing [Evidence IEA]; GO_process:
GO:0006364 - rRNA processing [Evidence IMP] [PMID
11694595]; GO_process: GO:0042274 - ribosomal small
subunit biogenesis [Evidence IMP] [PMID 11694595];
GO_process: GO:0042254 - ribosome biogenesis [Evidence
IEA]"
/codon_start=1
/product="Nop14p"
/protein_id="XP_018737097.1"
/db_xref="GeneID:30034113"
/translation="MAGSQLKQLKASLKANGLLGQTNTGKKGKRPNSSHDRKDRDKAI
QSIREAFNPFDVKVTKQKVDVIGRTIQGAQGKPGISKQVGEENRRRAYEVMAKKNKAG
GIIDRRFGENDANMTPEEKMLARFTREKLTGLASRKESLFNLEDDDGEDDTLTHYGQS
LSLTDDFEQDDLGVDEDDEETKEIIRKRRMMMVNGNEEEEGESSAPKSKKEVMQEIIA
KSKMYKYERQQAKEEDLHIIDELDAQENLDELLEDLNAYDRKHTVPKAESAERDEEYE
QRVREMAFDRRAQPTDRTKTEEELAKEKAEKTRKLEQDRLARMDGEIYNDDDDDIGHT
SDSGSGEEDEGEGDEDVNDAAEFGLDVRNFSDDYVSDGDNIDGSDEGEFLDEDDEEAE
SELSQPSKRRKVSESKPQYTCPETIDDLESILNQQTSSSEYPKVIERILDLYHPSKAV
GNKQKLGVFTTVLFEFLQKPNYTSNYPQVFTKMVEQLKTLTTSHVEVLTDFCREQILQ
VNEKLTATRLGDSTVAPADVLLFTLIGLVFSTSDHFHLVVTSATLVLAQHLSQSPLRS
AKDLLTNIFICDTLLAYQRISQRYIPEIPLFLQRLLTLSTSSEGAKDVAETLDIFVED
SDFHFDLDIKALKGLDGTLKLLDFVEPRDNLLERIFLHSLDTISTASNLWRDKLAYIE
IFGPITKLLEVHGDIHPLVASTRSKLEKLIAISTKERVPLTLQSHRPLPIQTFAPKFE
ENYNVDKKSYDPDRERNEGNKLRAELKKERKGALRDLRRDNAFIAREKIKTKRQTDKE
YHEKLARLERTIQTEEGAEKNKYEREKQARKRKH"
gene complement(<734402..>738736)
/gene="mus7"
/locus_tag="AWJ20_2226"
/db_xref="GeneID:30034114"
mRNA complement(<734402..>738736)
/gene="mus7"
/locus_tag="AWJ20_2226"
/product="DNA repair protein Mus7/Mms22"
/transcript_id="XM_018879156.1"
/db_xref="GeneID:30034114"
CDS complement(734402..738736)
/gene="mus7"
/locus_tag="AWJ20_2226"
/codon_start=1
/product="DNA repair protein Mus7/Mms22"
/protein_id="XP_018737098.1"
/db_xref="GeneID:30034114"
/translation="MPLEDGYISDSEGSLYDEVITRSLTAYEPPDYELDHNSRIPLTN
EVQSENQRLNADVQQQSDLLLEELSGNGFESGANIELARKPQSSRESATQNKIDLPPG
SQEDREQPQLPIIPDSSITTTISAESSTSDGLSTEHPHTAQTSLEENEIVNAVAIVEA
RSGRSLRQRTVLQLHPYTLEYELYRRSLKSRGIRPVVVPSQGKSSGPDPQLPQNSSSQ
QETPSQSQLYPESMGDDMNSEDIAYEENNETFGTTFSSPPPSSPIPPVSLPSKTTKGR
AAKLKALDPDEHLYANAFSQGSSAASSEDEAIDRILLAKQRRRRQLFKTIARPETESH
SRPNSGTDSADSSKFNSSSDSGESDSESEPELASEIELLKKKVRGVLPPSFITIDYTL
QHQNKNKTNKKKTNSSTENNHKGVARKKIGNSTQRPLSPAPFGFTSSRTASPPAYTRT
TPKAPSLTPPVLASPIRTDSVDNNHRPILISSDDDFGLPGQFEPLDDLVDPSDMEDDH
IDTMNGPSKGGSSRNPQTRPRKRLAQSTIDGNSKDTRYKHKRPRGSVRHTNHQTRITG
KSTKRRPQGHHNSKVTAEARQSQNSTTQQMSIVDLIDNSTSTERNNMPRFSLIAQRSA
LLKAHEQRLDSPTHKYIVINDGDESHPELKTLKRWKRGRLRQTNKLGSYSNVRQHHQS
VRSDNNYRTAPTSVRERKEQSKPRRKLPTQLLTKKNPSLSVVSRGNSYYQSKSPQFRT
TIIEIESGEYSVAKPMNSVRPKQYPALSPLQSISPGMRPMYPLRHENLPRTHSNSSLS
FASSRPEVENNRVTREYKSKKQILRPPQRRTEYDDTQLVGDMDHLQPITSLVGAPHLS
SMDAVPNRPFQGRRFYSYQFDVQPFAHDVVFATDTIVYNCVVQRPLGSKRDAIFLGNG
IGLVEAKVEDHGLRIPKISEAYNRFHIWIEDCLSPEVASKDYSLDVYEFFLFVLPLVS
DGNSPSYDQDFVKELSRLTLGLVADILDVWSRPFANTKMSTASSEFAQLTFTTASLCL
LLLHRLKDLLKTDPTTMYSHSNAIRNLGGKLVQLIVSNFLPEVSNIMRSHRSTSNRVI
TRTSYILEIIYIAIHVLDNEVKGDGFYDMLNSVITLPQLLRNPEDLDSVERVWHSVFI
FSAVYKLPKLTLQSNWKLVNILQGLTLTTFDSPGQQTLAIPYQRVFLVRCLKLATDWK
WENNPALCISIYKFFAGRRFSNIEPSVPSGLSPFLSNGNFTEVVTTDSAYNIFLKLVA
WTICFFQGDDNRLRKFIGILTPLNGRLYPMSSGLKVTDLDALGNQYSLLLVQFKYGRP
STRPTIDHFRSFIVLEQSHILARNLSLEAWFVVTKLLLEQDMDLDDTMKWFAEIVDSA
LKDTRKTGRYTSNLERELDYQNVVTYQKFFEKAVVSIDKLVSNQAVISKSRQWMNLLT
PCKYRQIP"
gene <741159..>742592
/gene="CLD1"
/locus_tag="AWJ20_2227"
/db_xref="GeneID:30034115"
mRNA <741159..>742592
/gene="CLD1"
/locus_tag="AWJ20_2227"
/product="Cld1p"
/transcript_id="XM_018879157.1"
/db_xref="GeneID:30034115"
CDS 741159..742592
/gene="CLD1"
/locus_tag="AWJ20_2227"
/inference="similar to AA sequence:KEGG_Orthology:K13535"
/note="Mitochondrial cardiolipin-specific phospholipase;
functions upstream of Taz1p to generate
monolyso-cardiolipin; transcription increases upon
genotoxic stress; involved in restricting Ty1
transposition; has homology to mammalian CGI-58;
GO_component: GO:0005743 - mitochondrial inner membrane
[Evidence IDA] [PMID 23637464]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 19244244];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0004623 - phospholipase A2 activity
[Evidence IDA,IMP] [PMID 19244244]; GO_function:
GO:0016740 - transferase activity [Evidence IEA];
GO_process: GO:0035965 - cardiolipin acyl-chain remodeling
[Evidence IMP] [PMID 19244244]; GO_process: GO:0035965 -
cardiolipin acyl-chain remodeling [Evidence IMP] [PMID
23637464]; GO_process: GO:0032048 - cardiolipin metabolic
process [Evidence IDA,IGI] [PMID 19244244]"
/codon_start=1
/product="Cld1p"
/protein_id="XP_018737099.1"
/db_xref="GeneID:30034115"
/translation="MAEVQQAPASAAINEPSSATQTKSGGRSVTTGAVKKERHVLTYR
ESFSQWLKAPSHEEAERNVLSLLDFFPESDGKRVAKVNKVDIGNKLHINEFEIVNVEK
PDDVTEAGSEYSKNLVVLHGYGAGLALFYRNFAEWSSIKGSRTMALDLLGFGRSSRPK
FSIATKDVSARDENGRFKVVVETENWFIDALEKWRIAKNLDKFTLMGHSMGGYLAAAY
AFKYPSRVERLIMVSPAGVERGYTPELDNVRLFGGNSTDSLPKQSAKNKPSSSRQSSD
APHIDEEVSVSQSDITHHHHNELHPSDSHSSLVNEASTSNRKLPNWFMYLWNRNISPF
VLLRSAAIFAPRMTSNWTSRRFSDVSEKERDIMHMYAYKTFTAPGSGEYGLTRLLAAG
AVAREPLVDRVVKGLKCPSVWIYGENDWMNVHAGEEAVLRLNKLGTSSAANAKSHVID
YAGHHVYLDNPSDFDRVVVDFLKTTNH"
gene <742913..>743299
/locus_tag="AWJ20_2228"
/db_xref="GeneID:30034116"
mRNA <742913..>743299
/locus_tag="AWJ20_2228"
/product="oxidoreductase"
/transcript_id="XM_018879158.1"
/db_xref="GeneID:30034116"
CDS 742913..743299
/locus_tag="AWJ20_2228"
/inference="similar to AA sequence:KEGG_Orthology:K16066"
/note="NADP(+)-dependent serine dehydrogenase and carbonyl
reductase; acts on serine, L-allo-threonine, and other
3-hydroxy acids; green fluorescent protein fusion protein
localizes to the cytoplasm and nucleus; may interact with
ribosomes, based on co-purification experiments;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0016021 - integral component
of membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_component: GO:0005840 - ribosome [Evidence IDA] [PMID
16702403]; GO_function: GO:0004090 - carbonyl reductase
(NADPH) activity [Evidence IDA] [PMID 18630881];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IDA] [PMID 17962934];
GO_function: GO:0031132 - serine 3-dehydrogenase activity
[Evidence IDA] [PMID 12535615]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0008152 -
metabolic process [Evidence IC] [PMID 12535615];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA]"
/codon_start=1
/product="oxidoreductase"
/protein_id="XP_018737100.1"
/db_xref="GeneID:30034116"
/translation="MKFSVSSFVFKSLTSTLARNSIRKMSSYGSSAGSRIAGKTVLIT
GASAGIGEATAYELAEASGGDIRLILSARRVENLSKISKDLEAKYPKVKVLPLALDVS
KYKEIPQWVKDIPQEWSNVDVLINNA"
gene <743406..>743729
/locus_tag="AWJ20_2229"
/db_xref="GeneID:30034117"
mRNA <743406..>743729
/locus_tag="AWJ20_2229"
/product="oxidoreductase"
/transcript_id="XM_018879159.1"
/db_xref="GeneID:30034117"
CDS 743406..743729
/locus_tag="AWJ20_2229"
/inference="similar to AA sequence:KEGG_Orthology:K16066"
/note="NADP(+)-dependent serine dehydrogenase and carbonyl
reductase; acts on serine, L-allo-threonine, and other
3-hydroxy acids; green fluorescent protein fusion protein
localizes to the cytoplasm and nucleus; may interact with
ribosomes, based on co-purification experiments;
GO_component: GO:0005737 - cytoplasm [Evidence IDA] [PMID
14562095]; GO_component: GO:0016021 - integral component
of membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_component: GO:0005840 - ribosome [Evidence IDA] [PMID
16702403]; GO_function: GO:0004090 - carbonyl reductase
(NADPH) activity [Evidence IDA] [PMID 18630881];
GO_function: GO:0016491 - oxidoreductase activity
[Evidence IEA,IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IDA] [PMID 17962934];
GO_function: GO:0031132 - serine 3-dehydrogenase activity
[Evidence IDA] [PMID 12535615]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0008152 -
metabolic process [Evidence IC] [PMID 12535615];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA]"
/codon_start=1
/product="oxidoreductase"
/protein_id="XP_018737101.1"
/db_xref="GeneID:30034117"
/translation="MVHGNDKVGDIAPEDIDIMYHTNVLGLITLTQQFIPKFRAQGHG
DVINIGSIAGRDPYPGGAIYCSTKAALRSFTETLRKETIDTRIRVIEIQPGAVETEFS
SKLRN"
gene complement(<744276..>745268)
/locus_tag="AWJ20_2230"
/db_xref="GeneID:30034119"
mRNA complement(<744276..>745268)
/locus_tag="AWJ20_2230"
/product="hypothetical protein"
/transcript_id="XM_018879161.1"
/db_xref="GeneID:30034119"
CDS complement(744276..745268)
/locus_tag="AWJ20_2230"
/inference="similar to AA sequence:KEGG_Orthology:K00450"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737102.1"
/db_xref="GeneID:30034119"
/translation="MTPTSTSTERSQQQSKLIDSLPPHNVEPLWTVMSAMVPPVPQPK
AIPNVWNYKELRPLLLESGRVVNEHEAERRVLMLINPALTAPQTTDTLYAGLQLINPG
ETAPAHRHQAFALRFIIEGSGGFTAVEGKKLYMERGDVILTPRWEWHDHGKEGEGPMI
WLDGLDLPMFQSIPVNFAEGYAEKRYPSTPEVGDSPIKYPWAEVQKTLDSSSSSHAIY
NYVSKVKGKQGKHLSTIIGAQAERISPSSSSPLRQESSSFIVHVYSGKGYTVVNHNEQ
EQKLIWNQHDTFCIPSWVKFQHFNHSDDEQAYLFSYSDTPLLENLALHRVAKFD"
gene <745659..>746543
/gene="FMP41"
/locus_tag="AWJ20_2231"
/db_xref="GeneID:30034120"
mRNA <745659..>746543
/gene="FMP41"
/locus_tag="AWJ20_2231"
/product="Fmp41p"
/transcript_id="XM_018879162.1"
/db_xref="GeneID:30034120"
CDS 745659..746543
/gene="FMP41"
/locus_tag="AWJ20_2231"
/note="hypothetical protein; GFP-fusion protein is induced
in response to the DNA-damaging agent MMS; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0046872 - metal ion binding
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0006950 -
response to stress [Evidence IEA]"
/codon_start=1
/product="Fmp41p"
/protein_id="XP_018737103.1"
/db_xref="GeneID:30034120"
/translation="MVAWKRLIRFVATDGKVYRGEPIISENQDLGKLAEDGTKLSAKL
IKGDDIFSEDTVVTNEVLEVSKLLGPLAPTDVPIVKCVGLNYMKHIQEGGRTPPPYPS
IFYKPNFAVADHGEDIPIPKISQENQLDYEGELCVVIGKTGKDIKEEDALSYVAGYTA
GNDVSARTWQRDPKYAGGVPQWGFSKSFDKYAPLGPVLVSTSEIQNPGTLELKTLVNG
EVRQHTNTDDLLFNVAKIIAFISQGTTLEKGTVIMTGTPSGVAMGMKPTPKYLHANDV
VEVYISSIGTLKNKMHFI"
gene complement(<746887..>747297)
/locus_tag="AWJ20_2232"
/db_xref="GeneID:30034121"
mRNA complement(<746887..>747297)
/locus_tag="AWJ20_2232"
/product="hypothetical protein"
/transcript_id="XM_018879163.1"
/db_xref="GeneID:30034121"
CDS complement(746887..747297)
/locus_tag="AWJ20_2232"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737104.1"
/db_xref="GeneID:30034121"
/translation="MSAEGSCLCGAVGFTAPTTPIATLACYCSDCRKGSGHLGQILAK
YNESDVKIHDDKKVLKEYIVTATQSGFIKKKLFCGECGCTLLTIPTKHEGLYMVRSTL
MDDPEIWRNNFVPAKALFDDSRKAYLANVDTQIV"
gene <748030..>750690
/gene="ASG1"
/locus_tag="AWJ20_2233"
/db_xref="GeneID:30034122"
mRNA <748030..>750690
/gene="ASG1"
/locus_tag="AWJ20_2233"
/product="Asg1p"
/transcript_id="XM_018879164.1"
/db_xref="GeneID:30034122"
CDS 748030..750690
/gene="ASG1"
/locus_tag="AWJ20_2233"
/note="Zinc cluster protein proposed to be a
transcriptional regulator; regulator involved in the
stress response; null mutants have a respiratory
deficiency, calcofluor white sensitivity and slightly
increased cycloheximide resistance; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 14562095];
GO_function: GO:0003677 - DNA binding [Evidence IEA,IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0043565 - sequence-specific DNA
binding [Evidence IDA] [PMID 19111667]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 19158363]; GO_function: GO:0000981 -
sequence-specific DNA binding RNA polymerase II
transcription factor activity [Evidence IEA]; GO_function:
GO:0008270 - zinc ion binding [Evidence IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0006357 - regulation of transcription from RNA
polymerase II promoter [Evidence IEA]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA,IEA]; GO_process: GO:0006950 - response to
stress [Evidence IEA]; GO_process: GO:0006366 -
transcription from RNA polymerase II promoter [Evidence
IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA,IEA]"
/codon_start=1
/product="Asg1p"
/protein_id="XP_018737105.1"
/db_xref="GeneID:30034122"
/translation="MDGIKKSHQQVDGQGLESHSIVGERVDRSPIQEYDDSMYNDSSS
TKRRRVNLACVQCRDRKVRCDGAQPSCGRCNSRGTNCTYAKKFARARVSAEYVRELEE
KLGLVGGSDSRDWSQAPTSPGPRTVSTISLEEATSGVGSAIPSQSEQQGPGVHSINEG
MFGENQSNSSQLLHHNSSFAVIPRTSQDSNMHSLGSNQLNANSISPSTTYSINSIDIS
PNGTADSNQPGTLSNSLKTSTDAGTSLSASISSRPKTVSSSSAFGDPEDPTTAADAMG
ATAKKTNARRQDFYGSSSAMSFMKLVEAAVNAGKGNSNMRANESEGSKNFAPHKYKMA
YSCAPSYLTGGSAKEEDWVVPPRRLADHYVQSYFTYVYSLYPFIHQTSFMTAYNSVWV
SDSEQTDTNNGNGNGTGTSRSDAFAKGSYGNNRINDGFDDSSDDELNDGHMFYCIMNM
VFAFGCLFSSGVESISRESSSQVYFDRAREHLNFDVLATGDSGSLILLQALLLIGQYL
QATQRPAACWNVVGLAIRVAQELGLHQEHHIASRPSVIEQELCRRLWYGCTLMDRIVS
MTFGRPLMVTQDFKVNPPAFLDDSYITDSGTITPPKSSPPSILGFYQQTILLYDILAD
ILQNVYDTHDSTAVDDDEQRLLVLTNILKTDCRLNKFKKNIPPHLQVEKILQHPPSNP
FARQTNVLQARILHLEIMLYRPIVLSNSTLKGKQSPLNNDSSLQLSLENTICRLCVMS
AINLIKLIFDNKDSPNVPAIWYNIFYIYTSASVLMAAKLRPSLKSELDSESMEAAWKM
ALHLLRIFEQTGPSATRCLRILEVLHDKISSASHHQSKPMFDFLPNLVDSEIDFGPDS
DLLYSMMNDAYGPFSSNTIDFHYLDNLPGL"
gene complement(<750899..>752398)
/gene="HOL1"
/locus_tag="AWJ20_2234"
/db_xref="GeneID:30034123"
mRNA complement(<750899..>752398)
/gene="HOL1"
/locus_tag="AWJ20_2234"
/product="Hol1p"
/transcript_id="XM_018879165.1"
/db_xref="GeneID:30034123"
CDS complement(750899..752398)
/gene="HOL1"
/locus_tag="AWJ20_2234"
/note="Putative transporter in the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; mutations in membrane-spanning domains permit
cation and histidinol uptake; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005886 - plasma
membrane [Evidence ISS] [PMID 8955402]; GO_function:
GO:0015665 - alcohol transmembrane transporter activity
[Evidence IMP] [PMID 2405251]; GO_function: GO:0022890 -
inorganic cation transmembrane transporter activity
[Evidence IMP] [PMID 8955402]; GO_process: GO:0006812 -
cation transport [Evidence IMP] [PMID 8955402];
GO_process: GO:0015850 - organic hydroxy compound
transport [Evidence IMP] [PMID 2405251]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Hol1p"
/protein_id="XP_018737106.1"
/db_xref="GeneID:30034123"
/translation="MSDLEIKHPNGSPFTDQEKNIGSQHEEQYPPPSELNTEYILQRH
GTLELDPMPSHDPLDPLNWPALKKHLHLALIAYSCMLVTFGAAGIVPAYPGMAEEYGV
SIPTCSYFTSAQILISGIVPIFTTPIMNKYGRKKIQVISCLCCCAANIGGGFAKTYGQ
QMATRVLVGLFVSPSLGLGAVLVTEMFFSHQRGSKNGWWSLMITLGTPGGPFIMGFVV
KYAAIKWVYFILAIMNFVAFLGWMIASETLWERDGIVVNEPDSLFKFKSKSNVTINLW
SFIAPLAEAREIRVFIPSLAYAVVFCYANIVLIVETPQVFGPKFGLDAVGLGLQMIAV
LVGSIIGEVMSGPLSDWWMTRCVRKRGVKVIEDRLTLSYIGFILAMVGIIVWGVRLEQ
ATPMKWNITPLVGAAIAACGNQIVTTILITYSIDVDYKRSIEIGNFINFFRQTYGFIG
PFYFPDMFDNLGFSGAGGLMAGLMGVVSLASVLALQLYFTKRAKKTLGI"
gene <753434..>754681
/locus_tag="AWJ20_2235"
/db_xref="GeneID:30034124"
mRNA <753434..>754681
/locus_tag="AWJ20_2235"
/product="hypothetical protein"
/transcript_id="XM_018879166.1"
/db_xref="GeneID:30034124"
CDS 753434..754681
/locus_tag="AWJ20_2235"
/inference="similar to AA sequence:KEGG_Orthology:K00480"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737107.1"
/db_xref="GeneID:30034124"
/translation="MVSVKNKFKIIVCGGGLGGLGCAIGLALNGHHVIVLEAASEINE
VGAGIQIPPNCVKVLNEYGVAEKLKKYVTKPANINLRRYSTGEILNATKLDPHMTETY
GFPYWLIHRADYQRVLWDYALSIGVAIKTCSRIDSVDDATHTVKLVDGTTYTGDLIVG
ADGIRSKVRDSAVVDEEVVLPTPSSNCAYRATVPAEEMLADPVIAHLMTDINSNCWIG
YRRHVMAYPIRNGKMYNIVMSHPGQAAVGKWAEPGDIEEMKNHYKNFDPVVVQLLTHV
KTVLKWVLADLPTLPRWVSKSGQTVLIGDAAHAMLPYLAQGAAQAIEDGACISSLLSQ
CETEKDIPDAMRKYEKRRKLRCEKIQRGARDNGYIWHMPDGEEQEQRDSDMKLQPADK
KNPNQWSDPLFQKWMFGWDAFRD"
gene complement(<754829..>756262)
/gene="CYB2"
/locus_tag="AWJ20_2236"
/db_xref="GeneID:30034125"
mRNA complement(<754829..>756262)
/gene="CYB2"
/locus_tag="AWJ20_2236"
/product="Cyb2p"
/transcript_id="XM_018879167.1"
/db_xref="GeneID:30034125"
CDS complement(754829..756262)
/gene="CYB2"
/locus_tag="AWJ20_2236"
/inference="similar to AA sequence:KEGG_Orthology:K00101"
/note="Cytochrome b2 (L-lactate cytochrome-c
oxidoreductase); component of the mitochondrial
intermembrane space, required for lactate utilization;
expression is repressed by glucose and anaerobic
conditions; GO_component: GO:0005758 - mitochondrial
intermembrane space [Evidence IEA]; GO_component:
GO:0005758 - mitochondrial intermembrane space [Evidence
IDA] [PMID 3004948]; GO_component: GO:0005758 -
mitochondrial intermembrane space [Evidence IDA] [PMID
6290489]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 11502169]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0070469 - respiratory chain [Evidence
IEA]; GO_function: GO:0010181 - FMN binding [Evidence
IEA]; GO_function: GO:0004460 - L-lactate dehydrogenase
(cytochrome) activity [Evidence IEA]; GO_function:
GO:0004460 - L-lactate dehydrogenase (cytochrome) activity
[Evidence IDA] [PMID 3004948]; GO_function: GO:0003824 -
catalytic activity [Evidence IEA]; GO_function: GO:0020037
- heme binding [Evidence IEA]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0016491
- oxidoreductase activity [Evidence IEA,IEA]; GO_process:
GO:0006089 - lactate metabolic process [Evidence IMP]
[PMID 8492799]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA]"
/codon_start=1
/product="Cyb2p"
/protein_id="XP_018737108.1"
/db_xref="GeneID:30034125"
/translation="MVKVLAEEIKKHTTKESCWVVLHGKVYDVTDFLSRHPGGQKAIL
RVAGKDATEEFDIIHPEGTLKSLSSSAYIGEVEDIENLKDTPETSNESKLGTVALPAL
DAMLNLDDFEKTAQKVISAKGWAYYYSASDDLVTKVYNNEVYRKVLLRPRIFKDVETV
STVSKIQGLVTTLPLFVSPAAMARLAHPSGEKGIAHACGSEGIVQMISNNASMKFEDI
VSDPIVSTQKFFYQLYVQNDRKISENVLSKVIATGRCHAIVLTLDAPTPGKREADERV
ANDGKTFSENSGGIAKTETSEGLGRALFAGTSARLLWDDLHWLRKQIPADMPIILKGL
QTYEDALIAATQWAPKGLVHGILLSNHGGRAMDQSQPPLMILQEIRKYAPEVFSSIEV
YVDGGIRRGTDVVKALCLGAKQVGIGRAALYGLAGYGEQGVQRTIQILREEIETCMRL
LGVNSVEELGPQHVNTRQLDNLVYSRL"
gene <761325..>762437
/locus_tag="AWJ20_2237"
/db_xref="GeneID:30034126"
mRNA <761325..>762437
/locus_tag="AWJ20_2237"
/product="protein MET30"
/transcript_id="XM_018879168.1"
/db_xref="GeneID:30034126"
CDS 761325..762437
/locus_tag="AWJ20_2237"
/codon_start=1
/product="protein MET30"
/protein_id="XP_018737109.1"
/db_xref="GeneID:30034126"
/translation="MLFSGLKQGVALSKITTGAMVLNVMIWEGSKQASSFGSNSGSSA
ASSLTSSVGSGYTSTSGSRTVSSSGSVSSGSDLSTLSPDSSAATGSKAIESGNLELPP
LSTGNSTAIVSCADKSIRFYSLTSGKLIRTIKGLHGSSAVRFIRRLSNTHLITAGLDG
RLIVSNFTTGEVSAIFRAHSSYITSLDVWGGYVVSTGFDKIVNVFKIIDESGALKNMA
SVKLDVCPTGAKIVALDPSNNDIIEKESMDDTLRESSPSSPGHTFKEGFIPAVILCKQ
DTTYMFFYSLHSLRLLNKVNLCDADYGSDSFTPLDIAVDEQRLRYAVATNHVPYMRII
TGFVGKTTIVNNLAVFSPQDQYSAPQLVWSKSGKVS"
gene complement(<762866..>763429)
/gene="ANT1"
/locus_tag="AWJ20_2238"
/db_xref="GeneID:30034127"
mRNA complement(<762866..>763429)
/gene="ANT1"
/locus_tag="AWJ20_2238"
/product="Ant1p"
/transcript_id="XM_018879169.1"
/db_xref="GeneID:30034127"
CDS complement(762866..763429)
/gene="ANT1"
/locus_tag="AWJ20_2238"
/note="Peroxisomal adenine nucleotide transporter;
involved in beta-oxidation of medium-chain fatty acid;
required for peroxisome proliferation; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 11914276];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016021 - integral
component of membrane [Evidence ISM] [PMID 12192589];
GO_component: GO:0005779 - integral component of
peroxisomal membrane [Evidence IDA] [PMID 11566870];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0005778 - peroxisomal membrane [Evidence
IEA]; GO_component: GO:0005777 - peroxisome [Evidence
IEA]; GO_function: GO:0000295 - adenine nucleotide
transmembrane transporter activity [Evidence IMP] [PMID
11390660]; GO_function: GO:0000295 - adenine nucleotide
transmembrane transporter activity [Evidence IDA] [PMID
11566870]; GO_process: GO:0015867 - ATP transport
[Evidence IDA] [PMID 11566870]; GO_process: GO:0006635 -
fatty acid beta-oxidation [Evidence IGI,IMP] [PMID
11390660]; GO_process: GO:0006635 - fatty acid
beta-oxidation [Evidence IGI,IMP] [PMID 11566870];
GO_process: GO:0006811 - ion transport [Evidence IEA];
GO_process: GO:0007031 - peroxisome organization [Evidence
IMP] [PMID 11390660]; GO_process: GO:0015992 - proton
transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="Ant1p"
/protein_id="XP_018737110.1"
/db_xref="GeneID:30034127"
/translation="MLGALAGALAQIFTIPISVVTTKQQTSDVQQSLVATAKDVIGED
GVSGLWRGLKASLVLVVNPSITYGSFERLKQVLFANKPTLSAGDNFLLGALSKAMATI
ATQPMIVAKVMQQSSDKRRRQYKSFVDALVYLSKNEGLKGLFKGLGPQISKGVLVQGL
LFMIKDQVELFLILFFRLIKQRLALAT"
gene complement(<763913..>764125)
/locus_tag="AWJ20_2239"
/db_xref="GeneID:30034128"
mRNA complement(<763913..>764125)
/locus_tag="AWJ20_2239"
/product="hypothetical protein"
/transcript_id="XM_018879170.1"
/db_xref="GeneID:30034128"
CDS complement(763913..764125)
/locus_tag="AWJ20_2239"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737111.1"
/db_xref="GeneID:30034128"
/translation="MAVISKQLITSATAATTVAAAPLSPWGKAVAGALAAMAANALVY
PLDIVKTRLQVQTEDDDLETGEKRVK"
gene <765230..>766144
/gene="ICL1"
/locus_tag="AWJ20_2240"
/db_xref="GeneID:30034130"
mRNA <765230..>766144
/gene="ICL1"
/locus_tag="AWJ20_2240"
/product="isocitrate lyase 1"
/transcript_id="XM_018879172.1"
/db_xref="GeneID:30034130"
CDS 765230..766144
/gene="ICL1"
/locus_tag="AWJ20_2240"
/inference="similar to AA sequence:KEGG_Orthology:K01637"
/note="Isocitrate lyase; catalyzes the formation of
succinate and glyoxylate from isocitrate, a key reaction
of the glyoxylate cycle; expression of ICL1 is induced by
growth on ethanol and repressed by growth on glucose;
GO_component: GO:0005575 - cellular_component [Evidence
ND]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_function: GO:0003824 - catalytic activity
[Evidence IEA]; GO_function: GO:0004451 - isocitrate lyase
activity [Evidence IEA,IEA]; GO_function: GO:0004451 -
isocitrate lyase activity [Evidence IMP,ISS] [PMID
1551398]; GO_function: GO:0016829 - lyase activity
[Evidence IEA]; GO_process: GO:0019752 - carboxylic acid
metabolic process [Evidence IEA]; GO_process: GO:0006097 -
glyoxylate cycle [Evidence IEA,IEA]; GO_process:
GO:0006097 - glyoxylate cycle [Evidence TAS] [PMID
11092862]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0006099 - tricarboxylic
acid cycle [Evidence IEA]"
/codon_start=1
/product="isocitrate lyase 1"
/protein_id="XP_018737112.1"
/db_xref="GeneID:30034130"
/translation="MSSPAAKFRQLLSEPGVIIAPGVYDGLSARAATSSKAFKALYMT
GAGTVASRLGAPDVGLASLPDMAANASLIANIDRSVPLIADADTGYGNSAMCARTLSL
YSQAGVAALHVEDQVHSKRCGHLLGKQLVSAEEFVSRIKAMAIERKRIQSDIVIIART
DSLQSLGVDEAIERSKQAVAVGADVIFVEGVENSEQAKKIVSALNPTPVLVNIVPNGV
TPNWSEEEIQELGFKIAIFPGVTLGPAFAAMSASLEALAKTRKDPTGAAAVAQKSSPK
EFFLRLGLGEIIALDKAVGGTAFEGSNI"
gene <766804..>768255
/locus_tag="AWJ20_2241"
/db_xref="GeneID:30034131"
mRNA <766804..>768255
/locus_tag="AWJ20_2241"
/product="hypothetical protein"
/transcript_id="XM_018879173.1"
/db_xref="GeneID:30034131"
CDS 766804..768255
/locus_tag="AWJ20_2241"
/inference="similar to AA sequence:KEGG_Orthology:K17724"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737113.1"
/db_xref="GeneID:30034131"
/translation="MITEPETASDAPSPTVELCKWVESLTWDDVPEEIRTRAKYLILD
GITCAIVASHLPWSETAVKAVSSIEGEGPCHVIGWKDLKVGPLQSALLNSTFIQGFEL
DDYHSFAPLHSNSIMVPAILALVSYGKHKGISYSGKDFLLAHVVGFEVGPRVGLALHG
GEILNRGWHSGPVFGHAASAAASSKFLGLNSEQIEDAFGIACTQTCGLMSAQFESSVK
RMQHGFASRNGLFAALMAEQSYKGIKRVFEREYGGFLNVFSLGAEPPVVEELTKDLSE
KWITPEINIKPYACMAGIHSTIDALYELFDKKPLQNAMDVVKQVTIEISEPLFKHGGW
KATKPLTVIGAQMNNAYICAVILLDGVLTVKNFSEKLMNRPEIWELMEKIDVVHNKEF
DTLPRTDRMIAAVKIEFTDGTIKTNTVVRPSGVKAPLSGDEIVDKFRTHTKGLISDEA
QANILKTVLEIESSKDVSELVDALNIDILDPLK"
gene <768910..>770523
/locus_tag="AWJ20_2242"
/db_xref="GeneID:30034132"
mRNA <768910..>770523
/locus_tag="AWJ20_2242"
/product="carboxylesterase-lipase family protein"
/transcript_id="XM_018879174.1"
/db_xref="GeneID:30034132"
CDS 768910..770523
/locus_tag="AWJ20_2242"
/codon_start=1
/product="carboxylesterase-lipase family protein"
/protein_id="XP_018737114.1"
/db_xref="GeneID:30034132"
/translation="MTSAPTIKRWEKHQLKFAGQGTLDGIKILEDDESGDVKCYRYTG
VRYAQAPVGPLRWRRPQKLSKTYDYTGDYTSFKTVCPQPSLSTISANDDIVYDEDCLF
ANIWVPGGTPPKEGWPVLYFIHGGFLQVGSPHHYRQADPQDLLAKDSKAKYIIVAAGY
RLNLFGFLSSKELLEEDKYNSNFGFWDQRMGMEWVYEHIHHFGGNKDNITVGGLSAGS
YSAIFQLAYELYHPEVTQIIKRSLLLSNGLAIQPNTVDKTQDQFDSLLKELDIPKSLS
AKEKLEKLRKVPFESLVKAIDGLDRQTFRAITDTYFVSPTLFDDIYSGKFGELVNTSG
RSLMSGEVDNEWLIYSLTNAPDTVDELAAKIENYYPRPVTNALIKHYPTPKPDDDDDL
QKVFGDIVSDMQVYVSSRLLVNSLAKAGMTTDRVFRYRIAFRGQYLDKYAPPEFRVPH
TGDIGIWFYTAFDGITDDEKPLFQQWLEPVAEWITTGTTAKWGTRSITDLRVFNRDGT
ITITPDDRWDWAMTVGRVVSSALSPGSKL"
gene complement(<770734..>771948)
/gene="CLB3"
/locus_tag="AWJ20_2243"
/db_xref="GeneID:30034133"
mRNA complement(<770734..>771948)
/gene="CLB3"
/locus_tag="AWJ20_2243"
/product="B-type cyclin CLB3"
/transcript_id="XM_018879175.1"
/db_xref="GeneID:30034133"
CDS complement(770734..771948)
/gene="CLB3"
/locus_tag="AWJ20_2243"
/inference="similar to AA sequence:KEGG_Orthology:K06659"
/codon_start=1
/product="B-type cyclin CLB3"
/protein_id="XP_018737115.1"
/db_xref="GeneID:30034133"
/translation="MKSASHSPQTGPYRRRPRKVNDHALDMMAKEMSQQVHSEYGQDI
IAHMDKMQRLTAADAGLMDLQPEIEWHMRPFLLDFLIDTHVALRLQPQTLFLAINLID
RYCSMRVVYRKHYQLVGCTALWIAAKYEDKKSRIPTINDLTNMCCKAYEGDMFVQMEG
HVLNTLNWTIGHPTVQSYLDVLVASESTSPTVRHVAQYLCEISLYHRAFISLTPSAIA
SAAFNLAKHLLTANDHAPFPVYLSSEENQTMSLLYQHMNSPSKSLHRKYSSSTLSQAT
HLVSSFLARQQRASLRMPPTPPPSSLLTNLTPPSLSSSVSSATNSPGESPIYATTARI
IPQHSKTMHIKIPAPFKSEGYLTPPCTPDDINGPLNNRSNSIASVASAASYHHNSAFT
SVPMEYTQSMET"
gene <782255..>783319
/gene="TNA1"
/locus_tag="AWJ20_2244"
/db_xref="GeneID:30034134"
mRNA <782255..>783319
/gene="TNA1"
/locus_tag="AWJ20_2244"
/product="Tna1p"
/transcript_id="XM_018879176.1"
/db_xref="GeneID:30034134"
CDS 782255..783319
/gene="TNA1"
/locus_tag="AWJ20_2244"
/note="High affinity nicotinic acid plasma membrane
permease; responsible for uptake of low levels of
nicotinic acid; expression of the gene increases in the
absence of extracellular nicotinic acid or
para-aminobenzoate (PABA); GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0005887 -
integral component of plasma membrane [Evidence ISS] [PMID
9678606]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0015663 - nicotinamide mononucleotide transmembrane
transporter activity [Evidence IMP] [PMID 10869563];
GO_process: GO:0015890 - nicotinamide mononucleotide
transport [Evidence IEP,IMP] [PMID 10869563]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Tna1p"
/protein_id="XP_018737116.1"
/db_xref="GeneID:30034134"
/translation="MMAFRFLMGVFETGYSPGVPYYLTFFYYRHEIAWRISVFCVAAP
IATTFSGALAYGITKNTNLAIASWRVLFLVESLPTIALGLWGYYALPNSSADSRFLTE
HEKNIAKARLARQISKVEMSRSFRVKDFLTSLIDPKVVLPMVMYFSINVSYSSLPVFT
PAIIHGMGFTSVNSQGLSAIPYIYTTVIVLLSCYLSDRWRVRGAFVTILSLKGAVGWL
LLALCKTTGVRYFALFLASSGIFSCVPLMLTWMSNNQGTEQKRGIGFMFINILGQTGP
LVGTRLFPASEAPLYIKGCWISFGFTVFLSTCSVVLALHLIYENRKLERKYGPAEDTT
QASNSVSEDGEVNPNFRYIW"
gene complement(<783493..>783963)
/locus_tag="AWJ20_2245"
/db_xref="GeneID:30034135"
mRNA complement(<783493..>783963)
/locus_tag="AWJ20_2245"
/product="hypothetical protein"
/transcript_id="XM_018879177.1"
/db_xref="GeneID:30034135"
CDS complement(783493..783963)
/locus_tag="AWJ20_2245"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737117.1"
/db_xref="GeneID:30034135"
/translation="MSRSLSKPSDFTGGIELEKIIASNRTKPLVIFIEGTTSNGKGLL
PFVSNVPISALPANTDIYPSVLKYTPPDPSTTPIPGSLPGWVIKVLSTQMTITARIKF
ANKLSTNPQNNEAAFANEIADSITRVGRIRKLGPAVDLAAKREFLSAWYRGRSS"
gene <784705..>785826
/gene="TAZ1"
/locus_tag="AWJ20_2246"
/db_xref="GeneID:30034136"
mRNA <784705..>785826
/gene="TAZ1"
/locus_tag="AWJ20_2246"
/product="Taz1p"
/transcript_id="XM_018879178.1"
/db_xref="GeneID:30034136"
CDS 784705..785826
/gene="TAZ1"
/locus_tag="AWJ20_2246"
/inference="similar to AA sequence:KEGG_Orthology:K13511"
/note="Lyso-phosphatidylcholine acyltransferase; required
for normal phospholipid content of mitochondrial
membranes; major determinant of the final acyl chain
composition of the mitochondrial-specific phospholipid
cardiolipin; mutations in human ortholog tafazzin cause
Barth syndrome, a rare X-linked disease characterized by
skeletal and cardiomyopathy and bouts of cyclic
neutropenia; GO_component: GO:0016021 - integral component
of membrane [Evidence IEA]; GO_component: GO:0031307 -
integral component of mitochondrial outer membrane
[Evidence IDA] [PMID 16135531]; GO_component: GO:0016020 -
membrane [Evidence IEA]; GO_component: GO:0005743 -
mitochondrial inner membrane [Evidence IDA] [PMID
16880272]; GO_component: GO:0005741 - mitochondrial outer
membrane [Evidence IEA,IEA]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
16880272]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 15304507]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 15588229]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0047184 - 1-acylglycerophosphocholine O-acyltransferase
activity [Evidence IEA]; GO_function: GO:0047184 -
1-acylglycerophosphocholine O-acyltransferase activity
[Evidence IMP] [PMID 15588229]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_function:
GO:0016746 - transferase activity, transferring acyl
groups [Evidence IEA,IEA]; GO_process: GO:0035965 -
cardiolipin acyl-chain remodeling [Evidence IMP] [PMID
14651618]; GO_process: GO:0032048 - cardiolipin metabolic
process [Evidence IMP] [PMID 14651618]; GO_process:
GO:0007007 - inner mitochondrial membrane organization
[Evidence IMP] [PMID 14651618]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0042775 -
mitochondrial ATP synthesis coupled electron transport
[Evidence IMP] [PMID 15304507]; GO_process: GO:0008654 -
phospholipid biosynthetic process [Evidence IMP] [PMID
14651618]; GO_process: GO:0008654 - phospholipid
biosynthetic process [Evidence IMP] [PMID 15588229];
GO_process: GO:0006461 - protein complex assembly
[Evidence IMP] [PMID 16135531]"
/codon_start=1
/product="Taz1p"
/protein_id="XP_018737118.1"
/db_xref="GeneID:30034136"
/translation="MSFQQISNNGYKFLTHYPRQNKYWKAASKATLGAVGLWSKLTLG
AISTPKMHNTDILTEAYRTSKLENRGLLTVMNHTSVLDEPLVWGGLLPVRHYFMKDGL
RWALGADNVCFKNSFTKMFFSLGQVLATKRFGVGPFQGSIDASINLLSSNQTKYSAES
ESNNGKGGIVLSASAASDSDSIPMNKSIIDRPGWVHIFPETFVHQPYPPHQHTLKYFH
WGVSRLILESSQPPIVVPIYTHGLEKIIPEDESYIGGLRKRLGSKVEFNVGMPLDESA
VASFRKEWLGMVRDQHKQGAIQALDISNNIYESLNEPLMTGTAARDLRSRVAAFVREG
VDATRLQLGFEQSDPRMADASFWHNQKEVRLAGKMHNRS"
gene <786879..>787613
/gene="HFI1"
/locus_tag="AWJ20_2247"
/db_xref="GeneID:30034137"
mRNA <786879..>787613
/gene="HFI1"
/locus_tag="AWJ20_2247"
/product="Hfi1p"
/transcript_id="XM_018879179.1"
/db_xref="GeneID:30034137"
CDS 786879..787613
/gene="HFI1"
/locus_tag="AWJ20_2247"
/note="Adaptor protein required for structural integrity
of the SAGA complex; a histone
acetyltransferase-coactivator complex that is involved in
global regulation of gene expression through acetylation
and transcription functions; GO_component: GO:0005671 -
Ada2/Gcn5/Ada3 transcription activator complex [Evidence
IDA,IPI] [PMID 9154821]; GO_component: GO:0000124 - SAGA
complex [Evidence IDA] [PMID 9674426]; GO_component:
GO:0070461 - SAGA-type complex [Evidence IEA];
GO_component: GO:0046695 - SLIK (SAGA-like) complex
[Evidence IDA] [PMID 12446794]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_function: GO:0003713 -
transcription coactivator activity [Evidence IMP] [PMID
10048020]; GO_function: GO:0003712 - transcription
cofactor activity [Evidence IMP,IPI] [PMID 9154821];
GO_process: GO:0016568 - chromatin modification [Evidence
IDA] [PMID 9674426]; GO_process: GO:0016573 - histone
acetylation [Evidence IDA] [PMID 9674426]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006366 - transcription
from RNA polymerase II promoter [Evidence IMP] [PMID
9154821]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Hfi1p"
/protein_id="XP_018737119.1"
/db_xref="GeneID:30034137"
/translation="MASPALLMNGNLAKNILNGGSSAGSAASVGTPGPSNGGGLNLEG
NGMTGAITPAKTISGSALSTNPTLTVPTKAISANSPQAQSMTGNTSSSKRLDVDTIMI
EFQKSLGDNWDRYRDVITSFLIGRLTRFELQEELDQILDKNAIKLHNHFLLTNLANSL
RDPPPGEQGSLSGWFKKQKDGARNVKGDSQLAKLKEDILGLSVRERKRIKAIARVRKR
HNQPLFQARGSNPESLNSRASTPDSS"
gene <787761..>788573
/gene="HFI1"
/locus_tag="AWJ20_2248"
/db_xref="GeneID:30034138"
mRNA <787761..>788573
/gene="HFI1"
/locus_tag="AWJ20_2248"
/product="Hfi1p"
/transcript_id="XM_018879180.1"
/db_xref="GeneID:30034138"
CDS 787761..788573
/gene="HFI1"
/locus_tag="AWJ20_2248"
/note="Adaptor protein required for structural integrity
of the SAGA complex; a histone
acetyltransferase-coactivator complex that is involved in
global regulation of gene expression through acetylation
and transcription functions; GO_component: GO:0005671 -
Ada2/Gcn5/Ada3 transcription activator complex [Evidence
IDA,IPI] [PMID 9154821]; GO_component: GO:0000124 - SAGA
complex [Evidence IDA] [PMID 9674426]; GO_component:
GO:0070461 - SAGA-type complex [Evidence IEA];
GO_component: GO:0046695 - SLIK (SAGA-like) complex
[Evidence IDA] [PMID 12446794]; GO_component: GO:0005634 -
nucleus [Evidence IEA,IEA]; GO_function: GO:0003713 -
transcription coactivator activity [Evidence IMP] [PMID
10048020]; GO_function: GO:0003712 - transcription
cofactor activity [Evidence IMP,IPI] [PMID 9154821];
GO_process: GO:0016568 - chromatin modification [Evidence
IDA] [PMID 9674426]; GO_process: GO:0016573 - histone
acetylation [Evidence IDA] [PMID 9674426]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006366 - transcription
from RNA polymerase II promoter [Evidence IMP] [PMID
9154821]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Hfi1p"
/protein_id="XP_018737120.1"
/db_xref="GeneID:30034138"
/translation="MLPKIPFINEKDKAKLQSGGTAASGAAGSGSSAATPASPNKQNK
GASPAVDTSSSSSNNNHSLANPMVWTQDIIHSYEAPLASEIYELPDNDSLNARMLGIS
LEHGLLHGIDNGVCDIMIAGLEHYLKDVVQQIFDKARLRKIPDSANTSIFEQSSPRTA
SVSGAASALTEPISKRGGSISQTTNPFSAPATSLAGSFEDTMTAEDMAMALDTAPHSF
VEMTGPVYRLKNTMLTDEESADPAERPPTPMFNSHIESQPEIANLLKDIMSS"
gene complement(<788880..>789293)
/locus_tag="AWJ20_2249"
/db_xref="GeneID:30034139"
mRNA complement(<788880..>789293)
/locus_tag="AWJ20_2249"
/product="hypothetical protein"
/transcript_id="XM_018879181.1"
/db_xref="GeneID:30034139"
CDS complement(788880..789293)
/locus_tag="AWJ20_2249"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737121.1"
/db_xref="GeneID:30034139"
/translation="MIIPNSSLGSESVPKRPYEQLKPVGLGSKFSKAPSASDYLCSLC
EASPAKYTCPKCLKLYCSLDCYKGPNHVKCSKLFQESKEQPAEKSSKEEQAKMLKILE
DFNLAEPNGWKYELAPELAKKLKIKKDKQTQEQDP"
gene <789627..>792329
/gene="AEP3"
/locus_tag="AWJ20_2250"
/db_xref="GeneID:30034141"
mRNA <789627..>792329
/gene="AEP3"
/locus_tag="AWJ20_2250"
/product="Pentatricopeptide repeat-containing protein
At1g63130, mitochondrial"
/transcript_id="XM_018879183.1"
/db_xref="GeneID:30034141"
CDS 789627..792329
/gene="AEP3"
/locus_tag="AWJ20_2250"
/codon_start=1
/product="Pentatricopeptide repeat-containing protein
At1g63130, mitochondrial"
/protein_id="XP_018737122.1"
/db_xref="GeneID:30034141"
/translation="MRALSRLAAGSLRPRLRNISQQNAGALLVCNSIGLELLEHRLLV
PSHGPTPTIIKRNYNPFKKQPKWESDTKKSKNTEECDQLARKLRESLASQADSKQVKK
IIFDLVNVSQHRVSGEYIPLIERALQKFHFLSKKSKLLSIDEVQTLYRSVVQLDATAG
SNMDADKDKVDPARGWLLQSSVSQSSSESTLITKLYSVLYNSQSQLPTTDRVQNGLLY
MKYLRSENLIVESRKLLTALIQDATILASLDEPTILQFTSIIQNINPDVDSLIALYEI
LGSTGSLSLTLFHEGILAFTEIASKHPENESTQQLMKFLNSTIFIDSKLEPTRETIII
ALDCCLTLSSAECGKRLLRTLVKPRLDDLLADDSDPESSLQFYELLLMSCSKFGEDVA
TGKKIVDRILTSFTPDQMAKETWDVLAQWTVYYSPDTDVLETLIDQMVEHGFNPDEVT
LSDVVSIAIGAAKRTDKYIDSVVNMFNLKFDVDSNVQTFAFLIDRKLHSCDLEAAQKL
FQNSITASGCDWGVDSQRHIPTLDRLLVALCTSPPVDQRNVFEVYQQVLMFTRTVGYN
AQCELLKMFLSIGNSYDVELFIKEQFGDRPGLPWQAYSEIYHVMFDYIMTCKDYKLAW
DIYGLLNGLIKLPYESYYTAMELFCRLGRPDAALLILKYLRVRSRKEAIPSPDRNMYI
LLFNEFGKTLYEEGVQELQGLLRTDLYTESDIEIMNSILGAYCNLQDPHRTKNTWLEI
NGFPEGQGVNNDTITIMIKHLTRYSLPEVEKLWVSFPETYNLTPDADNLRQYVIANCY
HGYYMRALEVTKHMEQTYGIVPGRDIIEALYNWTMLDSRKKLVEKWAIETHPDTWKQL
QESNSLKTYVLPDNVDNDSEDNLRAQTIQTMEADDHKNSTLITR"
gene <801844..>804561
/gene="ROX1"
/locus_tag="AWJ20_2251"
/db_xref="GeneID:30034142"
mRNA <801844..>804561
/gene="ROX1"
/locus_tag="AWJ20_2251"
/product="Rox1p"
/transcript_id="XM_018879184.1"
/db_xref="GeneID:30034142"
CDS 801844..804561
/gene="ROX1"
/locus_tag="AWJ20_2251"
/note="Heme-dependent repressor of hypoxic genes; mediates
aerobic transcriptional repression of hypoxia induced
genes such as COX5b and CYC7; repressor function regulated
through decreased promoter occupancy in response to
oxidative stress; contains an HMG domain that is
responsible for DNA bending activity; involved in the
hyperosmotic stress resistance; GO_component: GO:0000790 -
nuclear chromatin [Evidence IC] [PMID 10446242];
GO_component: GO:0000790 - nuclear chromatin [Evidence IC]
[PMID 10487921]; GO_component: GO:0000790 - nuclear
chromatin [Evidence IC] [PMID 7565763]; GO_component:
GO:0000790 - nuclear chromatin [Evidence IC] [PMID
8413209]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_function: GO:0003677 - DNA binding [Evidence
IEA]; GO_function: GO:0008301 - DNA binding, bending
[Evidence IDA] [PMID 10446242]; GO_function: GO:0008301 -
DNA binding, bending [Evidence IDA] [PMID 7565763];
GO_function: GO:0001078 - RNA polymerase II core promoter
proximal region sequence-specific DNA binding
transcription factor activity involved in negative
regulation of transcription [Evidence IMP] [PMID
10446242]; GO_function: GO:0001135 - RNA polymerase II
transcription factor recruiting transcription factor
activity [Evidence IMP] [PMID 14665463]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 10446242]; GO_function: GO:0043565 -
sequence-specific DNA binding [Evidence IDA] [PMID
10487921]; GO_function: GO:0043565 - sequence-specific DNA
binding [Evidence IDA] [PMID 19111667]; GO_function:
GO:0043565 - sequence-specific DNA binding [Evidence IDA]
[PMID 8413209]; GO_process: GO:0000122 - negative
regulation of transcription from RNA polymerase II
promoter [Evidence IMP] [PMID 10446242]; GO_process:
GO:0000122 - negative regulation of transcription from RNA
polymerase II promoter [Evidence IMP] [PMID 10487921];
GO_process: GO:0000122 - negative regulation of
transcription from RNA polymerase II promoter [Evidence
IGI,IMP] [PMID 16835445]; GO_process: GO:0000122 -
negative regulation of transcription from RNA polymerase
II promoter [Evidence IMP] [PMID 2546055]; GO_process:
GO:0000122 - negative regulation of transcription from RNA
polymerase II promoter [Evidence IMP] [PMID 2847035];
GO_process: GO:0000122 - negative regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 3062365]; GO_process: GO:0097201 - negative
regulation of transcription from RNA polymerase II
promoter in response to stress [Evidence IMP] [PMID
21299653]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Rox1p"
/protein_id="XP_018737123.1"
/db_xref="GeneID:30034142"
/translation="MSRTDDSNEHDTNKNNLPRVYQNVVTSTTNTSSANPPQQQPITT
TTITSGNLCVCANNTPRVPRPRNAFILYRQHQHALVVQEHPGKSNPEISKIIGEQWRS
LPQKEKDFWIQLGDEEKKSHLERFPDYRYQPRRSSKRGGSTSGTSTPTGTGQIGEATI
CPNCKGLIINGPGYPNSNANNNNNVNVNALANATSNITTAVTVNPNKPLDFGNSAPGA
GQTSGTATTPGPGVPQSSISSPAIPSPPLRPYQSAPGTQTPPLPYQQHPQQQQQQHPQ
QQQQHSQQHPQQQQQQPQSHVQHSSLPPQHSSSVPPPLHYDNKQYPYQPPHQSPHQLS
HQPQHQPPHQTPHQPPHQPQHPSQSYYPYPPPPPPPSQTHVNTLTSLTQVVGSHRASA
PQVPSAHQTILPNHQQQGGPRHQSAPEAFPLNPPPLYSSRSPTVSSSSLLTDTSSIAT
SHSSISSASSSSAPGSISSASGVSGNLLPRIVPRPQDEILGTLPGSIPKSPIARQLSA
GSPFKPRSVSSPAPEGLHPPPEIHPRHSSLDRHASPSWNYPTQSASQSPIPSAATSPS
LGSTVPNSSQGISSGTGSTFQLYNRRQTSSVGLRPSASSHSLSSMTHGPGSSIPHAGP
VSLLAEEEKSAKRRRTSSHYDPLGPGPAATTNSGPPPSHYHHHVTSPHHGTSRLNQAS
APPAPSPVETFGFREKADLLSTICEPLHHHHHHRSTSIVETDRKGRIIAVEGKDLELV
EAITERLGARLEGSNCKYTPHLFEQKPYFMYTKSAVNMFKVSCLHQLVSELNLSHFKY
GIIGGYLMTLADELADQESPLTAAEQTSHRLTYRHRWMTNVNVLRGLPGPDYLILIDQ
DNQADATSVSVTTLNGGSKIIVATLGNLRTNEVIDRAVDGMINGITGKE"
gene complement(<805065..>805475)
/gene="VTI1"
/locus_tag="AWJ20_2252"
/db_xref="GeneID:30034143"
mRNA complement(<805065..>805475)
/gene="VTI1"
/locus_tag="AWJ20_2252"
/product="Vti1p"
/transcript_id="XM_018879185.1"
/db_xref="GeneID:30034143"
CDS complement(805065..805475)
/gene="VTI1"
/locus_tag="AWJ20_2252"
/inference="similar to AA sequence:KEGG_Orthology:K08493"
/note="Protein involved in cis-Golgi membrane traffic;
v-SNARE that interacts with two t-SNARES, Sed5p and
Pep12p; required for multiple vacuolar sorting pathways;
GO_component: GO:0005794 - Golgi apparatus [Evidence IEA];
GO_component: GO:0000139 - Golgi membrane [Evidence IEA];
GO_component: GO:0031201 - SNARE complex [Evidence IPI]
[PMID 10397773]; GO_component: GO:0030173 - integral
component of Golgi membrane [Evidence IDA] [PMID 9398683];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0031902 - late endosome
membrane [Evidence IEA]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0005484 -
SNAP receptor activity [Evidence IDA] [PMID 19805279];
GO_process: GO:0006896 - Golgi to vacuole transport
[Evidence IMP] [PMID 9199167]; GO_process: GO:0006891 -
intra-Golgi vesicle-mediated transport [Evidence IMP]
[PMID 9398683]; GO_process: GO:0006886 - intracellular
protein transport [Evidence IEA]; GO_process: GO:0015031 -
protein transport [Evidence IEA]; GO_process: GO:0006810 -
transport [Evidence IEA]; GO_process: GO:0042144 - vacuole
fusion, non-autophagic [Evidence IMP] [PMID 10385523];
GO_process: GO:0006906 - vesicle fusion [Evidence IDA]
[PMID 11739407]; GO_process: GO:0016192 - vesicle-mediated
transport [Evidence IEA]"
/codon_start=1
/product="Vti1p"
/protein_id="XP_018737124.1"
/db_xref="GeneID:30034143"
/translation="MAIEVQNIPSSNRSKFNSKLREYRTNVENARKELRKLSETSDRR
ALFGDRLASGSPDNIPLDQRQQLLSGQASLERSSQRLRDSQRVANETESIGANILSDL
RGQREQLTNSRNVLLEADGYVDKSIRTLRGMARR"
gene complement(<805559..>805786)
/locus_tag="AWJ20_2253"
/db_xref="GeneID:30034144"
mRNA complement(<805559..>805786)
/locus_tag="AWJ20_2253"
/product="hypothetical protein"
/transcript_id="XM_018879186.1"
/db_xref="GeneID:30034144"
CDS complement(805559..805786)
/locus_tag="AWJ20_2253"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737125.1"
/db_xref="GeneID:30034144"
/translation="MSSPLDSGAGTELFASYDSDFQLAYSEITQKLDDIPNLTTEQQK
PAIKAAERAVEEAYEIVSRKPEVASPINPSL"
gene <806909..>808876
/locus_tag="AWJ20_2254"
/db_xref="GeneID:30034145"
mRNA <806909..>808876
/locus_tag="AWJ20_2254"
/product="Peroxisomal biogenesis factor 8"
/transcript_id="XM_018879187.1"
/db_xref="GeneID:30034145"
CDS 806909..808876
/locus_tag="AWJ20_2254"
/codon_start=1
/product="Peroxisomal biogenesis factor 8"
/protein_id="XP_018737126.1"
/db_xref="GeneID:30034145"
/translation="MSYLTDITQVVDSIVRELQNSSIYSSSGPADLARRKALIGRAIQ
YLPSLRNEHNVPLLALELLSSPVVALPEPVCSDFLVDGFRSAVARKYSISDPTIPPQE
WTKYLVRPVIDRLTAEGALWRILPLLSGLIQASKYEILADIERSERGLRQSKITYVPS
VADLRYQQDWYVRVFNQIITLPLESDSDYKKYRDIINMALITLAKCQWALDPDVYSRL
VHAETIVRSLQLIYSYPDTSVINSLRLQSSPVIELVNNLLGGFSNIIRQSVESNYRNN
LSFNDLDLALNHIHMFAEDLSRTWVRLKHSPDSITKSETEQWQILKQFIFAICLQLQG
FAGLLLHVNRPKLVPVAYLSAKILKTLSPVYFILDKIGSATFEPFSFTYNVCIDVLTL
SRGTLRQAQESVDSLIRALAGSCNLGAVLDNDMDRGKILFLIDLFEKLIHFSSFQVIN
QIILPFIGEFLVPNRDPTNRKLLTPLLESSHSVMLQYLRLSPEVAVINDNVVMNYLDT
LLALFPHDLSSPQLLLALKTIAQSVAAPTRPTKQLLHQFLERLYFKCKITLPAIPLTT
KAEENGPPTVRSVFVSSLIHSLPLLEPGDFQFWLERTDKLFLRRGPYYSPLFQAENEY
LTEDMEKMISGELDLSLADIGIRWWHHSSKL"
gene complement(<808942..>810183)
/gene="MMM1"
/locus_tag="AWJ20_2255"
/db_xref="GeneID:30034146"
mRNA complement(<808942..>810183)
/gene="MMM1"
/locus_tag="AWJ20_2255"
/product="ERMES complex subunit MMM1"
/transcript_id="XM_018879188.1"
/db_xref="GeneID:30034146"
CDS complement(808942..810183)
/gene="MMM1"
/locus_tag="AWJ20_2255"
/inference="similar to AA sequence:KEGG_Orthology:K17764"
/codon_start=1
/product="ERMES complex subunit MMM1"
/protein_id="XP_018737127.1"
/db_xref="GeneID:30034146"
/translation="MSTHHSYDISTSRVIPTPTYPAIVVANDDFLPPPTPPQGTSAAV
SFAYGLLVGQVSVLIVVVAFIRFFIFTDTSSERKDYVTALSRRDQRNAAKLMGANNVP
SENVINTILEKTYYNVETHNAESVDWFTVLIAQVVSQFREDARADDSMLRSLNDLLNG
DIIPDFIDKIRVTELNIGDDYPIFSNCKILYNRNELGQSQGQDGLEAQIDVDLTDTVT
LGIETRLLLNFPKALFAVLPVSLSVSIVRFSGTLSVSLRKSPAVPKAPNTVPASSNRS
ANATSTSNSDDANDGTTYLSFSFDPNYRLEFDIKSLVGARSRLQDIPKIGQFIESRLR
KWFTDRCVSPRCQQIPLPSFWPRSKTTAVVPPATNANTNGTNNVNPSGVNGLPPSSPL
VLPVDVAESLRRRRQSHLEVR"
gene <810952..>812676
/gene="KRI1"
/locus_tag="AWJ20_2256"
/db_xref="GeneID:30034147"
mRNA <810952..>812676
/gene="KRI1"
/locus_tag="AWJ20_2256"
/product="Kri1p"
/transcript_id="XM_018879189.1"
/db_xref="GeneID:30034147"
CDS 810952..812676
/gene="KRI1"
/locus_tag="AWJ20_2256"
/inference="similar to AA sequence:KEGG_Orthology:K14786"
/note="Essential nucleolar protein required for 40S
ribosome biogenesis; associate with snR30; physically and
functionally interacts with Krr1p; GO_component:
GO:0030686 - 90S preribosome [Evidence IDA] [PMID
22180534]; GO_component: GO:0005730 - nucleolus [Evidence
IEA]; GO_component: GO:0005730 - nucleolus [Evidence IDA]
[PMID 11027267]; GO_component: GO:0005634 - nucleus
[Evidence IEA]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0000447 -
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP] [PMID
11027267]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042254 - ribosome
biogenesis [Evidence IEA]"
/codon_start=1
/product="Kri1p"
/protein_id="XP_018737128.1"
/db_xref="GeneID:30034147"
/translation="MARKKSARRKALEAQAKEQGESPAKVENKDKLGLEEESDEDINT
LSINQEYAKRFEHNKSREEKHRQEEKDKAKLLEDSEEDAEDSSDDEDEDDFGELLTED
VDDGIQKVLETIRTNPKALLDPSVKFFDNSENAASANGNGTSKAMYLKDYHRQNLLSG
GVHDDDEENDNEEKPYAIQNREDRAKLLAEINKDDEDEEDDGDFLTKRKEQRTIEAIE
LPDPEQDQKGFLEAYTSSKAWIPSGIDKKTGKEIVPAYGDIVGDEDDEEFDDIADDFE
TAYNFRYEDPNAAEIVSYARNESTLRRKGDNSRKRAREQKTELKKQEEQSKKAEVNKI
KKKKVNEVLSKFEKLKEVIDDDETAAQFENMDLLEGDFDASEWDKRMEQVFNETFYSK
KDKNFAGDDKADEEDKDDETKEEPDEPKENIKEAEDEEPERRSRKDKHEAKKEKQKMR
QIAEQFVEANLDLALEQEKIDLVSSGTKFRYREVSPDSYGLTARDILLADDKDLNEFV
GIKKLAPFRDEEKKKHDRRKYAKKRRLREWRKAVFSTEDEPTDELFEKLVASQDAGQP
KKKKQRKR"
gene complement(<812915..>814231)
/gene="ARP4"
/locus_tag="AWJ20_2257"
/db_xref="GeneID:30034148"
mRNA complement(<812915..>814231)
/gene="ARP4"
/locus_tag="AWJ20_2257"
/product="Arp4p"
/transcript_id="XM_018879190.1"
/db_xref="GeneID:30034148"
CDS complement(812915..814231)
/gene="ARP4"
/locus_tag="AWJ20_2257"
/inference="similar to AA sequence:KEGG_Orthology:K11400"
/note="Nuclear actin-related protein involved in chromatin
remodeling; component of chromatin-remodeling enzyme
complexes; GO_component: GO:0031011 - Ino80 complex
[Evidence IPI] [PMID 10952318]; GO_component: GO:0031011 -
Ino80 complex [Evidence IPI] [PMID 24034245];
GO_component: GO:0035267 - NuA4 histone acetyltransferase
complex [Evidence IDA] [PMID 10911987]; GO_component:
GO:0000812 - Swr1 complex [Evidence IDA] [PMID 14645854];
GO_component: GO:0000812 - Swr1 complex [Evidence IDA]
[PMID 14690608]; GO_component: GO:0000812 - Swr1 complex
[Evidence IDA] [PMID 16299513]; GO_component: GO:0000790 -
nuclear chromatin [Evidence IDA] [PMID 10436015];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
10911987]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 11011149]; GO_function: GO:0043140 -
ATP-dependent 3'-5' DNA helicase activity [Evidence IDA]
[PMID 10952318]; GO_function: GO:0003682 - chromatin
binding [Evidence IDA] [PMID 10436015]; GO_function:
GO:0004402 - histone acetyltransferase activity [Evidence
IDA,IMP] [PMID 10911987]; GO_function: GO:0004402 -
histone acetyltransferase activity [Evidence IDA] [PMID
16088870]; GO_function: GO:0042393 - histone binding
[Evidence IDA] [PMID 10911987]; GO_function: GO:0031493 -
nucleosomal histone binding [Evidence IDA] [PMID
10911987]; GO_process: GO:0006281 - DNA repair [Evidence
IEA]; GO_process: GO:0006281 - DNA repair [Evidence IDA]
[PMID 12353039]; GO_process: GO:0006281 - DNA repair
[Evidence IMP] [PMID 15610740]; GO_process: GO:0006281 -
DNA repair [Evidence IDA] [PMID 16135807]; GO_process:
GO:0006974 - cellular response to DNA damage stimulus
[Evidence IEA]; GO_process: GO:0016568 - chromatin
modification [Evidence IEA]; GO_process: GO:0006325 -
chromatin organization [Evidence IMP,IPI] [PMID 10436015];
GO_process: GO:0006338 - chromatin remodeling [Evidence
IDA] [PMID 14645854]; GO_process: GO:0016573 - histone
acetylation [Evidence IDA] [PMID 10911987]; GO_process:
GO:0051382 - kinetochore assembly [Evidence IMP] [PMID
17452364]; GO_process: GO:0006357 - regulation of
transcription from RNA polymerase II promoter [Evidence
IDA] [PMID 10911987]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0006355 - regulation of transcription,
DNA-templated [Evidence IMP] [PMID 8598290]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Arp4p"
/protein_id="XP_018737129.1"
/db_xref="GeneID:30034148"
/translation="MEIASPMTDGIVSDWDATEKLWRYGLEDILGAQVTEQPLLITEQ
IWNTDENRKKAMEVAFESLDTPAFYIAKRPVCTLFASGKGSGLVVDIGADIASVTPVL
DGLTLFKTSKRSRHAGTYINKHIEALLKASDFDLTPRYLVAARQTGLEPGEKATEYKK
RSLLAPVTASFHDFQVSRLIEEFKESMAQVQDTPYTDEAASQAGTDVYSNRVFEFPDG
SSVEYGSERFKVAEPLFKPRDFLLEGEELLEAPEVKEEDGDDDDNKDDSKENGKDADS
KDKEKGKASKGSKDKGQDGDKDKDKEKPTEKSAATAAKELKALVYTDRTQGPFTTTGI
SDMIIDAINSCDVDIRANLANNVIITGGTSLIQGFTDRVNEDLVHALPGYKIRIHAPG
NLIERKFSAWIGGSILASLGTFHQLWISKKEYEEVGVSKLLEKRFR"
gene <815365..>817166
/gene="MIC60"
/locus_tag="AWJ20_2258"
/db_xref="GeneID:30034149"
mRNA join(<815365..815371,815470..>817166)
/gene="MIC60"
/locus_tag="AWJ20_2258"
/product="Mic60p"
/transcript_id="XM_018879191.1"
/db_xref="GeneID:30034149"
CDS join(815365..815371,815470..817166)
/gene="MIC60"
/locus_tag="AWJ20_2258"
/inference="similar to AA sequence:KEGG_Orthology:K17785"
/note="Component of the MICOS complex; MICOS (formerly
MINOS or MitOS) is a mitochondrial inner membrane complex
that extends into the intermembrane space and has a role
in the maintenance of crista junctions, inner membrane
architecture, and formation of contact sites to the outer
membrane; Mic60p is also involved in import of
intermembrane space (IMS) proteins, probably by
positioning Mia40p relative to the TOM complex to receive
incoming proteins; ortholog of mammalian mitofilin;
GO_component: GO:0061617 - MICOS complex [Evidence IDA]
[PMID 21944719]; GO_component: GO:0061617 - MICOS complex
[Evidence IDA] [PMID 21987634]; GO_component: GO:0061617 -
MICOS complex [Evidence IDA] [PMID 22009199];
GO_component: GO:0016021 - integral component of membrane
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0030061 - mitochondrial
crista [Evidence IDA] [PMID 19528297]; GO_component:
GO:0044284 - mitochondrial crista junction [Evidence IDA]
[PMID 22009199]; GO_component: GO:0005743 - mitochondrial
inner membrane [Evidence IEA,IEA]; GO_component:
GO:0005743 - mitochondrial inner membrane [Evidence IDA]
[PMID 19528297]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14562095]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0042407 -
cristae formation [Evidence IMP] [PMID 19528297];
GO_process: GO:0042407 - cristae formation [Evidence IMP]
[PMID 21944719]; GO_process: GO:0042407 - cristae
formation [Evidence IMP] [PMID 21987634]; GO_process:
GO:0042407 - cristae formation [Evidence IMP] [PMID
22009199]; GO_process: GO:0045041 - protein import into
mitochondrial intermembrane space [Evidence IMP,IPI] [PMID
21944719]"
/codon_start=1
/product="Mic60p"
/protein_id="XP_018737130.1"
/db_xref="GeneID:30034149"
/translation="MTVTPPVKVTPPVIEPVVKEAPKQKKSHGFRNFVLSTVFLSSAL
YGAGVWYSLKDDTFHDYFTEYVPFSESIIVAIEEREFRSKFRDVNINQGGATASTAVA
NGSGPLLQSIPGKLNDLKVKIRPGSSAEPKEISSDQATKSSSSSAVTAASSSSKSADK
NASIARPSSSDSSAKNIVTEKKEGIIPSGIGAPTAGGSSSHLLPLIRVPGDINPLVAS
SVETLNQFIQSVNTSTYGEDIVHKISQEVSKLSHSINALNKENQETLSKSLESQAAKF
AALGEARANEVKEALASQEDKWRQQFQEEQARILKLYNDRLLTEVEATKNLVISHANN
RLLAIHAEREKQFAQEIQDRVEKERDGRLSKIKELAEKVEALSHLTEKTSSIISESDS
VSQYHIAVGKLNSVLKFSNEPVPLGPYIEEVRKALSDDPLVQSILDNIPEDAYKEGVL
TNAQLAARFKLLVPELRSASLLPPNAGVAGHIGSIIFSQLLAGKSGNPVGNDNESIFA
RAETALGEGRVVDAVAEVNSLTGWPKKLAADWLSEGRKRSEVDFLAQALADEGKLWAL
K"
gene complement(<817387..>819852)
/gene="PAP2"
/locus_tag="AWJ20_2259"
/db_xref="GeneID:30034150"
mRNA complement(<817387..>819852)
/gene="PAP2"
/locus_tag="AWJ20_2259"
/product="non-canonical poly(A) polymerase PAP2"
/transcript_id="XM_018879192.1"
/db_xref="GeneID:30034150"
CDS complement(817387..819852)
/gene="PAP2"
/locus_tag="AWJ20_2259"
/inference="similar to AA sequence:KEGG_Orthology:K03514"
/note="Non-canonical poly(A) polymerase; involved in
nuclear RNA degradation as a component of TRAMP; catalyzes
polyadenylation of hypomodified tRNAs, and snoRNA and rRNA
precursors; required for mRNA surveillance and maintenance
of genome integrity, serving as a link between RNA and DNA
metabolism; overlapping but non-redundant functions with
Trf5p; relocalizes to cytosol in response to hypoxia; PAP2
has a paralog, TRF5, that arose from the whole genome
duplication; GO_component: GO:0031499 - TRAMP complex
[Evidence IDA] [PMID 15828860]; GO_component: GO:0031499 -
TRAMP complex [Evidence IDA] [PMID 15935758];
GO_component: GO:0031499 - TRAMP complex [Evidence IDA]
[PMID 15935759]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 22932476]; GO_component: GO:0005730 -
nucleolus [Evidence IDA] [PMID 16541108]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 10066793];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
22932476]; GO_function: GO:0051575 -
5'-deoxyribose-5-phosphate lyase activity [Evidence
IDA,IMP] [PMID 17983848]; GO_function: GO:0034459 -
ATP-dependent 3'-5' RNA helicase activity [Evidence IDA]
[PMID 22532666]; GO_function: GO:0003729 - mRNA binding
[Evidence IDA] [PMID 23222640]; GO_function: GO:0046872 -
metal ion binding [Evidence IEA]; GO_function: GO:0016779
- nucleotidyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0004652 - polynucleotide
adenylyltransferase activity [Evidence IDA] [PMID
12062100]; GO_function: GO:0004652 - polynucleotide
adenylyltransferase activity [Evidence IMP,ISS] [PMID
15828860]; GO_function: GO:0004652 - polynucleotide
adenylyltransferase activity [Evidence IDA] [PMID
15935758]; GO_function: GO:0004652 - polynucleotide
adenylyltransferase activity [Evidence IDA,IMP] [PMID
15935759]; GO_function: GO:0004652 - polynucleotide
adenylyltransferase activity [Evidence IDA,IMP] [PMID
16260630]; GO_function: GO:0004652 - polynucleotide
adenylyltransferase activity [Evidence IDA,IGI] [PMID
16374505]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_process: GO:0034475 - U4 snRNA 3'-end
processing [Evidence IGI,IMP] [PMID 16373491]; GO_process:
GO:0006284 - base-excision repair [Evidence IGI,IMP] [PMID
17983848]; GO_process: GO:0007049 - cell cycle [Evidence
IEA]; GO_process: GO:0051301 - cell division [Evidence
IEA]; GO_process: GO:0071044 - histone mRNA catabolic
process [Evidence IGI] [PMID 17179095]; GO_process:
GO:0007067 - mitotic nuclear division [Evidence IEA];
GO_process: GO:0043629 - ncRNA polyadenylation [Evidence
IGI] [PMID 15145828]; GO_process: GO:0043629 - ncRNA
polyadenylation [Evidence IDA,IMP] [PMID 15828860];
GO_process: GO:0043629 - ncRNA polyadenylation [Evidence
IDA] [PMID 15935758]; GO_process: GO:0043629 - ncRNA
polyadenylation [Evidence IGI] [PMID 15935759];
GO_process: GO:0043629 - ncRNA polyadenylation [Evidence
IGI] [PMID 16373491]; GO_process: GO:0043629 - ncRNA
polyadenylation [Evidence IGI] [PMID 16374505];
GO_process: GO:0043629 - ncRNA polyadenylation [Evidence
IMP] [PMID 16431988]; GO_process: GO:0045910 - negative
regulation of DNA recombination [Evidence IMP] [PMID
23762389]; GO_process: GO:0071031 - nuclear mRNA
surveillance of mRNA 3'-end processing [Evidence IGI]
[PMID 17410208]; GO_process: GO:0071039 - nuclear
polyadenylation-dependent CUT catabolic process [Evidence
IGI,IMP] [PMID 15935759]; GO_process: GO:0071039 - nuclear
polyadenylation-dependent CUT catabolic process [Evidence
IMP] [PMID 16973436]; GO_process: GO:0071039 - nuclear
polyadenylation-dependent CUT catabolic process [Evidence
IMP] [PMID 18007593]; GO_process: GO:0071039 - nuclear
polyadenylation-dependent CUT catabolic process [Evidence
IMP] [PMID 18591258]; GO_process: GO:0071040 - nuclear
polyadenylation-dependent antisense transcript catabolic
process [Evidence IMP] [PMID 18022365]; GO_process:
GO:0071042 - nuclear polyadenylation-dependent mRNA
catabolic process [Evidence IGI] [PMID 16373491];
GO_process: GO:0071035 - nuclear polyadenylation-dependent
rRNA catabolic process [Evidence IGI,IMP] [PMID 15935758];
GO_process: GO:0071035 - nuclear polyadenylation-dependent
rRNA catabolic process [Evidence IMP] [PMID 16431988];
GO_process: GO:0071035 - nuclear polyadenylation-dependent
rRNA catabolic process [Evidence IGI] [PMID 16541108];
GO_process: GO:0071035 - nuclear polyadenylation-dependent
rRNA catabolic process [Evidence IMP] [PMID 18007593];
GO_process: GO:0071037 - nuclear polyadenylation-dependent
snRNA catabolic process [Evidence IMP] [PMID 15935758];
GO_process: GO:0071037 - nuclear polyadenylation-dependent
snRNA catabolic process [Evidence IMP] [PMID 16431988];
GO_process: GO:0071036 - nuclear polyadenylation-dependent
snoRNA catabolic process [Evidence IGI,IMP] [PMID
15935758]; GO_process: GO:0071036 - nuclear
polyadenylation-dependent snoRNA catabolic process
[Evidence IGI] [PMID 16373491]; GO_process: GO:0071038 -
nuclear polyadenylation-dependent tRNA catabolic process
[Evidence IGI] [PMID 15145828]; GO_process: GO:0071038 -
nuclear polyadenylation-dependent tRNA catabolic process
[Evidence IDA] [PMID 15828860]; GO_process: GO:0071038 -
nuclear polyadenylation-dependent tRNA catabolic process
[Evidence IDA] [PMID 15935758]; GO_process: GO:0071038 -
nuclear polyadenylation-dependent tRNA catabolic process
[Evidence IMP] [PMID 16431988]; GO_process: GO:0071038 -
nuclear polyadenylation-dependent tRNA catabolic process
[Evidence IDA] [PMID 17643380]; GO_process: GO:0071038 -
nuclear polyadenylation-dependent tRNA catabolic process
[Evidence IMP] [PMID 18456844]; GO_process: GO:0071047 -
polyadenylation-dependent mRNA catabolic process [Evidence
IMP] [PMID 19369424]; GO_process: GO:0071051 -
polyadenylation-dependent snoRNA 3'-end processing
[Evidence IGI] [PMID 16373491]; GO_process: GO:0071050 -
snoRNA polyadenylation [Evidence IGI] [PMID 18951092];
GO_process: GO:0006400 - tRNA modification [Evidence IMP]
[PMID 22319136]"
/codon_start=1
/product="non-canonical poly(A) polymerase PAP2"
/protein_id="XP_018737131.1"
/db_xref="GeneID:30034150"
/translation="MGSKAKKSSPQLASQASSGSLNGLPVGPSAYVAAVEKGTADVGS
NNQPQKGLKKLLNKLPKGPKGDSKDQAGNQPKSKAKNKPKKNQPKNQPPEKKPKNQAK
SQPNLSKKMSENQLRNQTTSGVNGQIHASEKNHDNYSHQLKPSKSIGTESEMSKKSGI
DKIVDGMGNVIEKFKKRQRGSRGGKKRRASMDAIANDYISNVAASGVDHDDAVMDADI
NTNDDNEDSGFIAFDSDLSDVAESIHTNTFKAFEDEMSMFLSEDEDDDQEDNAVNVSI
MTTSTSRTSRKRKFNPEDEDEKASKRINVLSEFPWVVNHDHSQEQEISDWLTLEIKDF
VSYMSPSAAEIIARNNAVKRIRGIVKDLWSDADIHVFGSFATDLYLPGSDIDMVVLSK
SGSYDTRAHLYKLSSKLKATGIANSLVVVAKARVPIIKFVEKTSNIHIDISFERRNGV
EAVQIIRKWTAEYPCIRFFAIIIKHFLARRKMNEVHSGGLGGFSVICLIVSFLKNHPK
VSSGAIDPMHNLGVLLIEFFELYGRNFNYDSIALSVRGDMPYLRKDQNPDLDNPRKFM
LAIQDPSDETNNLSRGTFNIRGIKKAFSGAFDLLVARCYELEYLSFSDRLGKSILGNV
IKFRGPERDFLDSRSEVQSEYELSIENYTSFNGTENDTNDDIIVPSDFEEDESDADVD
GNDGEQQDVIGDIYKKQSNSRPNQKLASPPSRPKPNSPKSKPERKSPSTAEHDGDKRA
TRPAAVEYYNISDSDSDSNAERPNDGTRHTSPTQRGASSKSPITILSDDDASSAEDSR
SSSVIDKAQKRDYWLSKGAPTIT"
gene <821500..>823359
/gene="IKS1"
/locus_tag="AWJ20_2260"
/db_xref="GeneID:30034152"
mRNA <821500..>823359
/gene="IKS1"
/locus_tag="AWJ20_2260"
/product="protein kinase IKS1"
/transcript_id="XM_018879194.1"
/db_xref="GeneID:30034152"
CDS 821500..823359
/gene="IKS1"
/locus_tag="AWJ20_2260"
/codon_start=1
/product="protein kinase IKS1"
/protein_id="XP_018737132.1"
/db_xref="GeneID:30034152"
/translation="MSLLGLCPRPRGSSRFARVGRRRSQQLLPSRSNGVWGSAPAAGS
STNGSDRFFISSALLGRGSRGAVYLVEHVLDGFSLGFFALKKVAVGDDHAWLEKVLSE
VNLLRMLSHPNLVRYNHVWLEVSSLSSFGPLVPCAYILQEYCNGGTLEEYMMKGRYKP
GTDGESDIIPQFQRDPNDRTWTKNDIKAKRDRIRRRMSQQGADGQGYTDFSGRNGGSM
SAVVDDNDDEYVAELTIEEIISFMTDIVMGVKYLHQNQIIHRDLKPSNCLLCRIPGQS
LPTVLVSDFGEGQIEGLNRMGSGSTGTLEYTAPELVQTVSGRFVAQFSKKTDVFSLGM
ILHYLCFSRLPYSNVWQERGDLEALTGEVRQFGGFDINISNSKLHRTDLDPQLLALVD
AMVSIDPDRRPTADQVLFILGQVNERYRQQQFHQAQTTAMSTASQTSPPPASSTSGTS
TAAATTTAVPEITTNTTATSTSTALPINSTTATLRISDTSSSLMSPTLSPSINTPATS
SFDMSSSLSSSLVPTVIYSPDHQRPNMLLRYLPTWIQRLAIRNRRSPRQLTSSLLLTK
GTLLLIKLATIYKLAPSPYTQAIYMLLGVELATLSIPVSIGLFLAHLVLYSLA"
gene complement(<823599..>824246)
/gene="VPS21"
/locus_tag="AWJ20_2261"
/db_xref="GeneID:30034153"
mRNA complement(<823599..>824246)
/gene="VPS21"
/locus_tag="AWJ20_2261"
/product="Rab family GTPase VPS21"
/transcript_id="XM_018879195.1"
/db_xref="GeneID:30034153"
CDS complement(823599..824246)
/gene="VPS21"
/locus_tag="AWJ20_2261"
/inference="similar to AA sequence:KEGG_Orthology:K07889"
/note="Endosomal Rab family GTPase; required for endocytic
transport and sorting of vacuolar hydrolases; required for
endosomal localization of the CORVET complex; required
with YPT52 for MVB biogenesis and sorting; involved in
autophagy and ionic stress tolerance; geranylgeranylation
required for membrane association; protein abundance
increases in response to DNA replication stress; mammalian
Rab5 homolog; VPS21 has a paralog, YPT53, that arose from
the whole genome duplication; GO_component: GO:0005768 -
endosome [Evidence IEA]; GO_component: GO:0010008 -
endosome membrane [Evidence IEA]; GO_component: GO:0005770
- late endosome [Evidence IMP] [PMID 11872141];
GO_component: GO:0016020 - membrane [Evidence IEA];
GO_component: GO:0031966 - mitochondrial membrane
[Evidence IEA]; GO_component: GO:0005741 - mitochondrial
outer membrane [Evidence IDA] [PMID 16407407];
GO_component: GO:0005739 - mitochondrion [Evidence IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14576278]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 16823961]; GO_function: GO:0005525 -
GTP binding [Evidence IEA,IEA]; GO_function: GO:0003924 -
GTPase activity [Evidence IDA] [PMID 11210571];
GO_function: GO:0003924 - GTPase activity [Evidence IMP]
[PMID 8163546]; GO_function: GO:0000166 - nucleotide
binding [Evidence IEA]; GO_process: GO:0006895 - Golgi to
endosome transport [Evidence IGI,IMP] [PMID 22748138];
GO_process: GO:0006895 - Golgi to endosome transport
[Evidence IGI,IMP] [PMID 23612966]; GO_process: GO:0006897
- endocytosis [Evidence IMP] [PMID 8163546]; GO_process:
GO:0032511 - late endosome to vacuole transport via
multivesicular body sorting pathway [Evidence IGI] [PMID
22748138]; GO_process: GO:0036258 - multivesicular body
assembly [Evidence IGI] [PMID 22748138]; GO_process:
GO:0036010 - protein localization to endosome [Evidence
IGI] [PMID 23264632]; GO_process: GO:0006623 - protein
targeting to vacuole [Evidence IMP] [PMID 8163546];
GO_process: GO:0015031 - protein transport [Evidence
IEA,IEA]; GO_process: GO:0007264 - small GTPase mediated
signal transduction [Evidence IEA]; GO_process: GO:0006810
- transport [Evidence IEA]; GO_process: GO:0000011 -
vacuole inheritance [Evidence IMP] [PMID 1493335]"
/codon_start=1
/product="Rab family GTPase VPS21"
/protein_id="XP_018737133.1"
/db_xref="GeneID:30034153"
/translation="MSTPRTSTSSPRFAQFKLVLLGESAVGKSSLVNRFAKDQFDDFR
ESTIGAAFLTQTVQVDPNTIVKFEIWDTAGQERYKSLAPMYYRNANCAVVVYDITQSA
SLTRAKAWVKELQHQANENIIIALAGNKFDLQESRDVDTSVAEAYAKEAGLLFFETSA
KTGENVTELFTAIAKKLPIESVLNRSTRPSGPKGVDLSRSARQPSGANGAGACNC"
gene complement(<826242..>826967)
/gene="TAE1"
/locus_tag="AWJ20_2262"
/db_xref="GeneID:30034154"
mRNA complement(<826242..>826967)
/gene="TAE1"
/locus_tag="AWJ20_2262"
/product="Tae1p"
/transcript_id="XM_018879196.1"
/db_xref="GeneID:30034154"
CDS complement(826242..826967)
/gene="TAE1"
/locus_tag="AWJ20_2262"
/inference="similar to AA sequence:KEGG_Orthology:K16219"
/note="AdoMet-dependent proline methyltransferase;
catalyzes the dimethylation of ribosomal proteins Rpl12
and Rps25 at N-terminal proline residues; has a role in
protein synthesis; fusion protein localizes to the
cytoplasm; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 20481588]; GO_function: GO:0071885 -
N-terminal protein N-methyltransferase activity [Evidence
IDA,IMP] [PMID 20481588]; GO_function: GO:0008168 -
methyltransferase activity [Evidence IEA,IEA];
GO_function: GO:0016740 - transferase activity [Evidence
IEA]; GO_process: GO:0018016 - N-terminal peptidyl-proline
dimethylation [Evidence IDA,IMP] [PMID 20481588];
GO_process: GO:0002181 - cytoplasmic translation [Evidence
IGI,IMP] [PMID 19055778]; GO_process: GO:0032259 -
methylation [Evidence IEA]"
/codon_start=1
/product="Tae1p"
/protein_id="XP_018737134.1"
/db_xref="GeneID:30034154"
/translation="MPLEPDVPADTHINYDDAVSYWSSVPATVDGMLGGFGTGVVPKV
DIIGSNAFVRRLNILQKSASGDQLPRHGLDVGAGIGRITKDFLSKICDQVDLVEPVKN
FVDQAHESLADLKKEGKVGTIFNVGMQDFTPEEGKYWLIWCQWCVGHLADDRLVEFFQ
RCIKGLQPGGYIIVKENNAPVEDDFDDTDSSVTRTDAKFRELFTRAGLDLKLVTKQTG
LPRELYPVRMYALRPATDVEPSS"
gene <829631..>830581
/gene="SEC61"
/locus_tag="AWJ20_2263"
/db_xref="GeneID:30034155"
mRNA <829631..>830581
/gene="SEC61"
/locus_tag="AWJ20_2263"
/product="translocon subunit SEC61"
/transcript_id="XM_018879197.1"
/db_xref="GeneID:30034155"
CDS 829631..830581
/gene="SEC61"
/locus_tag="AWJ20_2263"
/inference="similar to AA sequence:KEGG_Orthology:K10956"
/note="Conserved ER protein translocation channel;
essential subunit of Sec61 complex (Sec61p, Sbh1p, and
Sss1p); forms channel for SRP-dependent protein import;
with Sec63 complex allows SRP-independent protein import
into ER; involved in posttranslational soluble protein
import into the ER, ERAD of soluble substrates, and
misfolded soluble protein export from the ER;
GO_component: GO:0005784 - Sec61 translocon complex
[Evidence IDA] [PMID 8612571]; GO_component: GO:0005784 -
Sec61 translocon complex [Evidence IDA] [PMID 9252322];
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_function: GO:0015450 -
P-P-bond-hydrolysis-driven protein transmembrane
transporter activity [Evidence IMP] [PMID 1550957];
GO_function: GO:0015450 - P-P-bond-hydrolysis-driven
protein transmembrane transporter activity [Evidence IDA]
[PMID 9252322]; GO_function: GO:0015197 - peptide
transporter activity [Evidence IMP] [PMID 10758167];
GO_function: GO:0005048 - signal sequence binding
[Evidence IDA] [PMID 12134063]; GO_process: GO:0030433 -
ER-associated ubiquitin-dependent protein catabolic
process [Evidence IPI] [PMID 19696741]; GO_process:
GO:0030433 - ER-associated ubiquitin-dependent protein
catabolic process [Evidence IMP] [PMID 24314051];
GO_process: GO:0006616 - SRP-dependent cotranslational
protein targeting to membrane, translocation [Evidence
IDA] [PMID 11076036]; GO_process: GO:0044743 -
intracellular protein transmembrane import [Evidence IMP]
[PMID 24314051]; GO_process: GO:0070843 - misfolded
protein transport [Evidence IMP] [PMID 24314051];
GO_process: GO:0031204 - posttranslational protein
targeting to membrane, translocation [Evidence IDA] [PMID
7758110]; GO_process: GO:0031204 - posttranslational
protein targeting to membrane, translocation [Evidence
IDA] [PMID 9252322]; GO_process: GO:0031204 -
posttranslational protein targeting to membrane,
translocation [Evidence IMP] [PMID 9843581]; GO_process:
GO:0015031 - protein transport [Evidence IEA,IEA];
GO_process: GO:0030970 - retrograde protein transport, ER
to cytosol [Evidence IMP] [PMID 10635318]; GO_process:
GO:0030970 - retrograde protein transport, ER to cytosol
[Evidence IMP] [PMID 9303298]; GO_process: GO:0006810 -
transport [Evidence IEA]"
/codon_start=1
/product="translocon subunit SEC61"
/protein_id="XP_018737135.1"
/db_xref="GeneID:30034155"
/translation="MGGAMLLLIDETLQKGYGFSTGFSLFAVLSVSTKFVWNALSLST
QDIGRGKEFIGAIVSLVHGLFTRNFKNALIESFYRTHLPNVFEVYGNILAFGAVVFLL
NFRYDINVKSTKVRTPSSNYPIRLLYTGNVPIYMFYAIVSNLFIFSNILFRLFPSNVV
VRLIGTWEQHGDNPQLFAVSGLCYYLQPPFGLVEALWDPIKTVIYASTLILAAIYFAK
TWTEISGSSPRDIAKQFKDNDIVIVGHRDVAILRELKKIITPAAAIGGALSAAVIVLP
DLLGFTGSGLLMFIGASTIYTYFEIIAQESGQPGFSVGNW"
gene <831318..>833534
/gene="MAK5"
/locus_tag="AWJ20_2264"
/db_xref="GeneID:30034156"
mRNA <831318..>833534
/gene="MAK5"
/locus_tag="AWJ20_2264"
/product="Mak5p"
/transcript_id="XM_018879198.1"
/db_xref="GeneID:30034156"
CDS 831318..833534
/gene="MAK5"
/locus_tag="AWJ20_2264"
/inference="similar to AA sequence:KEGG_Orthology:K14805"
/note="Essential nucleolar protein; putative DEAD-box RNA
helicase required for maintenance of M1 dsRNA virus;
involved in biogenesis of large (60S) ribosomal subunits;
GO_component: GO:0005730 - nucleolus [Evidence IEA];
GO_component: GO:0005730 - nucleolus [Evidence IDA] [PMID
13680366]; GO_component: GO:0005634 - nucleus [Evidence
IEA]; GO_function: GO:0005524 - ATP binding [Evidence
IEA,IEA]; GO_function: GO:0004004 - ATP-dependent RNA
helicase activity [Evidence ISS] [PMID 7754712];
GO_function: GO:0008026 - ATP-dependent helicase activity
[Evidence IEA]; GO_function: GO:0003723 - RNA binding
[Evidence IEA]; GO_function: GO:0004386 - helicase
activity [Evidence IEA,IEA]; GO_function: GO:0016787 -
hydrolase activity [Evidence IEA]; GO_function: GO:0003676
- nucleic acid binding [Evidence IEA]; GO_function:
GO:0000166 - nucleotide binding [Evidence IEA];
GO_process: GO:0006200 - ATP catabolic process [Evidence
IEA]; GO_process: GO:0000466 - maturation of 5.8S rRNA
from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [Evidence IMP] [PMID 13680366]; GO_process:
GO:0000463 - maturation of LSU-rRNA from tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [Evidence IMP]
[PMID 13680366]; GO_process: GO:0006364 - rRNA processing
[Evidence IEA]; GO_process: GO:0042273 - ribosomal large
subunit biogenesis [Evidence IMP] [PMID 13680366];
GO_process: GO:0042273 - ribosomal large subunit
biogenesis [Evidence IMP] [PMID 7739558]; GO_process:
GO:0042254 - ribosome biogenesis [Evidence IEA]"
/codon_start=1
/product="Mak5p"
/protein_id="XP_018737136.1"
/db_xref="GeneID:30034156"
/translation="MAGKALKRKLSSKSNGPQKSGLKKAKKTVALDNLAWKSVDVADG
GLDDFEGFYGLEEVDGVSVEVENGTLKFKAAESKEDTENEEKEKAPTNGSAKNKASEK
VAPYVSPFAEEEEDDYEDSDSEENQGEEEEEEGESEIDESKESKNKTAKGAENKKKSS
GLVSLKTPGNPFDLLEETESEVSAELDWSLPDTTLSSSILTALSALKFYKPTDIQAQV
IPEILTDSDVIGKAATGSGKTLAYGIPIIEKHIRLSESIKPAKKWPTALIFGPTRELV
HQITKHLEQVWKYGNFTGSGVVAITGGLAIQKQRRLIDNNPAIIVATPGRFLEILTEA
TSEFRDQFKKCETLVLDEADRLIQEGHFQELEKILDLIGRGRRTRRQTLVFSATFQRD
LMKKLDSKRTSSNAGRQASNAGALKILNEKLEFKDKNPVFIDSNPAESVARSVVESIM
ECGAMEKDLYLYYFLLTYPARSIVFVNSIDTVKRLVPFLKELGLPSLGLHSDMIQKQR
LRSLERFKANDKSILVATDVAARGLDIPLIDHVVHYHLPRTADMYIHRSGRTARAGNE
GVSLLLCSPDEASGPLIKLKRALYKDNATNKTAMKTFEVEYDFLNRLKPRITLAKQIS
DTVNQSTHKGKTDAWLKKAVEDLGVDLSDDEIENITGDRRKKNKPEQPKGDLRSLRAQ
LQSELQRPVGHAGKYLTRGTVNLAQMVTSGKSHQAFLGKEQTSALESLNTKPKSKK"
gene complement(<833707..>835023)
/gene="SUP45"
/locus_tag="AWJ20_2265"
/db_xref="GeneID:30034157"
mRNA complement(<833707..>835023)
/gene="SUP45"
/locus_tag="AWJ20_2265"
/product="translation termination factor eRF1"
/transcript_id="XM_018879199.1"
/db_xref="GeneID:30034157"
CDS complement(833707..835023)
/gene="SUP45"
/locus_tag="AWJ20_2265"
/inference="similar to AA sequence:KEGG_Orthology:K03265"
/note="Polypeptide release factor (eRF1) in translation
termination; mutant form acts as a recessive omnipotent
suppressor; methylated by Mtq2p-Trm112p in ternary complex
eRF1-eRF3-GTP; mutation of methylation site confers
resistance to zymocin; has a role in cytokinesis through
interaction with Mlc1p; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA,IEA]; GO_component: GO:0010494
- cytoplasmic stress granule [Evidence IDA] [PMID
23451152]; GO_component: GO:0005829 - cytosol [Evidence
IPI] [PMID 1474892]; GO_component: GO:0018444 -
translation release factor complex [Evidence IDA] [PMID
7556078]; GO_function: GO:0003747 - translation release
factor activity [Evidence IDA] [PMID 20947765];
GO_function: GO:0016149 - translation release factor
activity, codon specific [Evidence IEA]; GO_function:
GO:0016149 - translation release factor activity, codon
specific [Evidence IMP,ISS] [PMID 9182990]; GO_process:
GO:0006353 - DNA-templated transcription, termination
[Evidence IMP] [PMID 23109428]; GO_process: GO:0006412 -
translation [Evidence IEA]; GO_process: GO:0006415 -
translational termination [Evidence IEA]; GO_process:
GO:0006415 - translational termination [Evidence IGI,IMP]
[PMID 18724935]; GO_process: GO:0006415 - translational
termination [Evidence IDA] [PMID 20947765]; GO_process:
GO:0006415 - translational termination [Evidence IMP,ISS]
[PMID 9182990]"
/codon_start=1
/product="translation termination factor eRF1"
/protein_id="XP_018737137.1"
/db_xref="GeneID:30034157"
/translation="MAPAQEETDAEKNIQIWKVKKLIKNLEQARGNGTSMISLIIPPK
GQIPLTARMLADEYGTASNIKSRVNRLSVLSAITSTQQRLKLYNKVPPNGLVIYCGEI
ITEEGKERKVNIDFEPFKPINTSLYLCDNKFHTEALSELLESDSKFGFIVMDGNGALF
GTLSGNTREILHKFTVDLPKKHGRGGQSALRFARLRDEKRHNYVRKVAEVAVQNFITA
DKVNVTGLVLAGSADFKTELSQSDLFDNRLQAKVIKIVDVSYGGENGFNQAIELSAET
LSNVKFIQEKKLINAYFDEISQDSGKFCYGVDDTLKALDMGACETVIVFENLNIMRLT
LKSADGESTVVVHAAKDAPNKDHLLDKAGNELEVVEEKPLLEWLAENYQSFGATLEFV
TDRSSEGAQFVRGFGGIGAILRYKVNFEQLVDESDDEYFDDDDDYI"
gene complement(<835905..>837179)
/gene="STE24"
/locus_tag="AWJ20_2266"
/db_xref="GeneID:30034158"
mRNA complement(<835905..>837179)
/gene="STE24"
/locus_tag="AWJ20_2266"
/product="Ste24p"
/transcript_id="XM_018879200.1"
/db_xref="GeneID:30034158"
CDS complement(835905..837179)
/gene="STE24"
/locus_tag="AWJ20_2266"
/inference="similar to AA sequence:KEGG_Orthology:K06013"
/note="Highly conserved zinc metalloprotease; functions in
two steps of a-factor maturation, C-terminal CAAX
proteolysis and the first step of N-terminal proteolytic
processing; contains multiple transmembrane spans;
GO_component: GO:0005783 - endoplasmic reticulum [Evidence
IEA]; GO_component: GO:0005789 - endoplasmic reticulum
membrane [Evidence IEA]; GO_component: GO:0030176 -
integral component of endoplasmic reticulum membrane
[Evidence IDA] [PMID 11581258]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005741 -
mitochondrial outer membrane [Evidence IDA] [PMID
16407407]; GO_component: GO:0005637 - nuclear inner
membrane [Evidence IMP] [PMID 18923140]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0004222 - metalloendopeptidase
activity [Evidence IEA]; GO_function: GO:0004222 -
metalloendopeptidase activity [Evidence IDA] [PMID
11581258]; GO_function: GO:0004222 - metalloendopeptidase
activity [Evidence IMP,ISS] [PMID 9065405]; GO_function:
GO:0008237 - metallopeptidase activity [Evidence IEA];
GO_function: GO:0008233 - peptidase activity [Evidence
IEA]; GO_process: GO:0071586 - CAAX-box protein processing
[Evidence IMP] [PMID 9065405]; GO_process: GO:0007323 -
peptide pheromone maturation [Evidence IDA] [PMID
11581258]; GO_process: GO:0007323 - peptide pheromone
maturation [Evidence IMP] [PMID 9065405]; GO_process:
GO:0006508 - proteolysis [Evidence IEA,IEA]; GO_process:
GO:0019236 - response to pheromone [Evidence IEA]"
/codon_start=1
/product="Ste24p"
/protein_id="XP_018737138.1"
/db_xref="GeneID:30034158"
/translation="MGFTAGQYLFTTYLSYRQYQVLKKPSPPKELDNVITQETFKKTQ
EYGRAKAKFGFFTSFFSFVQNVAILKYDLLPKMFGLAGKFLAAYGHSKWFSGLTAQSI
VFLLGFQFVETIISLPLSLYSDFVIEERFGFNKQTIGLFFTDLVKSEILKIVIGAPLI
AAILKIIDYFGDQFFFYLWLFMVGFQVVAIAVFPTLIQPLFNKLSPLEDGELKTSVEN
LAKRVQFPLNKLYVIDGSKRSSHSNAYFYGLPWSKQIVIFDTLIEKSTVDEVTAVLAH
EIGHWALSHTTKMLLVAQAHLFAIFALFSAFIRNQSLYNSFGFFSSSSQYPILVGFIL
FGDILQPLESLLSFAMNLMSRSFEFQADDYAVNLNYGPELSKSLIGIHTENLSNVDAD
WLYSSYHHSHPILPERLKALRLATEAIQKKKH"
gene complement(<838466..>840064)
/gene="MUP1"
/locus_tag="AWJ20_2267"
/db_xref="GeneID:30034159"
mRNA complement(<838466..>840064)
/gene="MUP1"
/locus_tag="AWJ20_2267"
/product="Mup1p"
/transcript_id="XM_018879201.1"
/db_xref="GeneID:30034159"
CDS complement(838466..840064)
/gene="MUP1"
/locus_tag="AWJ20_2267"
/note="High affinity methionine permease; integral
membrane protein with 13 putative membrane-spanning
regions; also involved in cysteine uptake; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016021 - integral component of
membrane [Evidence ISM] [PMID 12192589]; GO_component:
GO:0005887 - integral component of plasma membrane
[Evidence ISS] [PMID 8893857]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA,IEA]; GO_function: GO:0000102 -
L-methionine secondary active transmembrane transporter
activity [Evidence IDA] [PMID 8893857]; GO_function:
GO:0015171 - amino acid transmembrane transporter activity
[Evidence IEA]; GO_process: GO:0003333 - amino acid
transmembrane transport [Evidence IEA]; GO_process:
GO:0006865 - amino acid transport [Evidence IEA];
GO_process: GO:0042883 - cysteine transport [Evidence IDA]
[PMID 11330701]; GO_process: GO:0044690 - methionine
import [Evidence IGI,IMP] [PMID 8893857]; GO_process:
GO:0015821 - methionine transport [Evidence IDA] [PMID
11330701]; GO_process: GO:0000101 - sulfur amino acid
transport [Evidence IMP] [PMID 8893857]; GO_process:
GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Mup1p"
/protein_id="XP_018737139.1"
/db_xref="GeneID:30034159"
/translation="MAFWESDKNSSTQVAKSDYESQKSSSPDSEIVVQEGGIQVIAPE
TSRSIGVVSATFLIFNRMIGTGIFATPGTIFGQCGSVGLTLFIWVVGALISAAGLMVY
LEWGSMIPKNGAEKAYLEYFYRKPRLLMTSLYGSYMFFMAWAASNSIVCGQYLLLAGG
VEPDRWNQRGVGLGCITFAFLVHGLALKWGLRIQNVLGIFKLIVIVIIVVIGWVAMSG
RLNIERTHAFHNAFENTNADAYGIVNALYNVMWSFIGYSNANYALAETKNPHRILKIA
APLAITIVTILYMFVNLAYFAVVPRDQILNSGTILAGNFFGIVWGKRGETAMSVFVAL
SALGNVMAVIFSQGRIVQELGREGSLPFSRFFASSRPLNSPLAGLFEHWLICVIIILA
VPPGDAYNFLVNLISYPYSIVNAFVALALIVIYINKKRYPEWNPPIKASLPVTVFFFL
SSVYLIAAPYVPPRSAADSVYKSLPYYLHCVVAWGIFAVGGIYWLVWARIIPRIRGYT
LDFEEYVDDDGWAKKKVVKIYKSD"
gene <842283..>843428
/locus_tag="AWJ20_2268"
/db_xref="GeneID:30034160"
mRNA <842283..>843428
/locus_tag="AWJ20_2268"
/product="hypothetical protein"
/transcript_id="XM_018879202.1"
/db_xref="GeneID:30034160"
CDS 842283..843428
/locus_tag="AWJ20_2268"
/inference="similar to AA sequence:KEGG_Orthology:K01078"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737140.1"
/db_xref="GeneID:30034160"
/translation="MLKLISLLIGFIAAVSGTPLFGILANPFAPKFSTIQPSNPEITT
AAACAPTSNYKDIEYVCGNVFDRIYIIFLENADYMKAVEDCHLKKLQKKGITLCNYWA
LTHTSEPNYIAAVGGDYYGLDTDDFVRLPERVFSIVDLLEYRNISWAEYQEHMPFTGY
QGFEYKNQCNGANDYVRKHNPLIMYDSVTNNARRLSNIKNFTELYSDVANRALPQWAF
ITCNMSNGGHDTTVAKAGEWAYNFLDQLLVDEYCFHKTLLVLTFDEVDRNPKNRVLAL
LLGDIPCDLQNTVDETYYDHYSLLSSVEANWDLPNLGRGDCGANTFEVIARTTGYKNK
QVDTRFKFNALSPPGWLAESFISIPPPDLNGQGAGGPILPEIARVWQ"
gene <847646..>849001
/gene="SDS3"
/locus_tag="AWJ20_2269"
/db_xref="GeneID:30034161"
mRNA <847646..>849001
/gene="SDS3"
/locus_tag="AWJ20_2269"
/product="Sds3p"
/transcript_id="XM_018879203.1"
/db_xref="GeneID:30034161"
CDS 847646..849001
/gene="SDS3"
/locus_tag="AWJ20_2269"
/note="Component of the Rpd3L histone deacetylase complex;
required for its structural integrity and catalytic
activity, involved in transcriptional silencing and
required for sporulation; relocalizes to the cytosol in
response to hypoxia; cells defective in SDS3 display
pleiotropic phenotypes; GO_component: GO:0033698 - Rpd3L
complex [Evidence IDA] [PMID 16286007]; GO_component:
GO:0033698 - Rpd3L complex [Evidence IDA] [PMID 16286008];
GO_component: GO:0033698 - Rpd3L complex [Evidence IDA]
[PMID 16314178]; GO_component: GO:0033698 - Rpd3L complex
[Evidence IDA] [PMID 19040720]; GO_component: GO:0070210 -
Rpd3L-Expanded complex [Evidence IDA] [PMID 19040720];
GO_component: GO:0005829 - cytosol [Evidence IDA] [PMID
22932476]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 22932476]; GO_function: GO:0004407 - histone
deacetylase activity [Evidence IMP] [PMID 11024051];
GO_process: GO:0016568 - chromatin modification [Evidence
IEA]; GO_process: GO:0006342 - chromatin silencing
[Evidence IGI,IMP] [PMID 11024051]; GO_process: GO:0016575
- histone deacetylation [Evidence IMP,IPI] [PMID
11024051]; GO_process: GO:0016575 - histone deacetylation
[Evidence IMP] [PMID 16314178]; GO_process: GO:0061188 -
negative regulation of chromatin silencing at rDNA
[Evidence IMP] [PMID 16286008]; GO_process: GO:0061186 -
negative regulation of chromatin silencing at silent
mating-type cassette [Evidence IMP] [PMID 16286008];
GO_process: GO:0031939 - negative regulation of chromatin
silencing at telomere [Evidence IMP] [PMID 16286008];
GO_process: GO:0031939 - negative regulation of chromatin
silencing at telomere [Evidence IMP] [PMID 19372273];
GO_process: GO:0045944 - positive regulation of
transcription from RNA polymerase II promoter [Evidence
IMP] [PMID 17210643]; GO_process: GO:0061408 - positive
regulation of transcription from RNA polymerase II
promoter in response to heat stress [Evidence IMP] [PMID
20398213]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Sds3p"
/protein_id="XP_018737141.1"
/db_xref="GeneID:30034161"
/translation="MDGTGRMAYAGDIHDQTGYGGGVVHGQYTHGQISGYGPGSAAAA
SVMAGGIGGVGVGVSGGIGAGGVGTGTGAGAGGGGAQPASKRDKRRQTLAAKLDQITD
MFENERDFHYSEMLRTLQSTLYSLHVGDNPEFLEELTDLEEVRDEQLVMLYLWEKYQI
ERADHEYNQDVELANEEHRQMTDMVKEKLMAKLESQRRKLSEDKTLLDIANDHSFFLS
SSGLYHSERNSAKDVNSDYGGGSSTAGAFLYPPGSPSGRDFRDGGGMSTRAAAGSGSA
AAAAIGAGGGGSGGTGYGIGGYGGTGLNGHVNGLGAGGEGFSSQSGMERRALRRRGGA
AYDDSGLSGGEGSGGAGGTGTGGATSTRRRGAYHGTLNGSGAANTNGYRTNNGGSDME
TAAAFSDRDLEGMLFSKDREAPTTRHSSKAYHGVRVLKPEEAQEDIDLLRASIRRAKR
P"
gene <850608..>852578
/gene="KTR5"
/locus_tag="AWJ20_2270"
/db_xref="GeneID:30034163"
mRNA <850608..>852578
/gene="KTR5"
/locus_tag="AWJ20_2270"
/product="Ktr5p"
/transcript_id="XM_018879205.1"
/db_xref="GeneID:30034163"
CDS 850608..852578
/gene="KTR5"
/locus_tag="AWJ20_2270"
/inference="similar to AA sequence:KEGG_Orthology:K03854"
/note="Putative mannosyltransferase involved in protein
glycosylation; member of the KRE2/MNT1 mannosyltransferase
family; KTR5 has a paralog, KTR7, that arose from the
whole genome duplication; GO_component: GO:0005794 - Golgi
apparatus [Evidence ISS] [PMID 9090056]; GO_component:
GO:0016021 - integral component of membrane [Evidence
IEA]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_function: GO:0000030 -
mannosyltransferase activity [Evidence IEA]; GO_function:
GO:0000030 - mannosyltransferase activity [Evidence ISS]
[PMID 9090056]; GO_function: GO:0016740 - transferase
activity [Evidence IEA]; GO_function: GO:0016757 -
transferase activity, transferring glycosyl groups
[Evidence IEA]; GO_process: GO:0097502 - mannosylation
[Evidence IC] [PMID 9090056]; GO_process: GO:0006486 -
protein glycosylation [Evidence IEA]"
/codon_start=1
/product="Ktr5p"
/protein_id="XP_018737142.1"
/db_xref="GeneID:30034163"
/translation="MVNLLKSLQSRLIYPAPSNTNHFDEDDDGEELFPTITSSAAMTP
VNHSQTGEYFPNYPRKSDSSTGSSNNDSGHGLGINSEKARLSSLSDNLSASASSSAGS
SPTMTSSPTHSSSSTNLKLLLEKHRSRKQNQPRSHTIRFIYKLSHKIRYLSAIRIRIK
HNVSVPLVVFLLPILSVLIFFLIFFLTRILHDNNSNNNNNNGGFFSKSPPKLREISDQ
YDKPFVVGCLEPQVDQPRANAVLVVLARNSEIEGVSQSMISLERHFNRWFNYPYVFLN
DEPFNSTFREAVRNITKSKVQFGTIDPVDWNFPEWAEPEDIKEGIAKQGDAAIMYGGM
ESYHHMCRFYSGKFYNHPLLASYEWYWRVEPEVKYFCDITYDPFLHMAKNNKVYGFTI
IIKELVETVPNLFRHTYGFKQNYGLNTTGLWDLFLTKPEDSDPDFKDANKDSGSVRKR
KKQQKKDKAKAKAEENAALSDILPEEAMLLEQGKEKGIQQTLQEEEKLDPYSMNGEKY
NMCHFWSNFEIARLDFFRSKQYNDYFETLDRSGGFWNERWGDAPVHSLAAGLFLEPEQ
IHYFRDIGYRHTTIQHCPANAPQRQRPHIPYIPDPTNKEQIEEDRYWANPDPEVLNGV
GCRCRCDTDIVDVEGKDGSCLADWVDLTGGWV"
gene complement(<853112..>854551)
/gene="RPN4"
/locus_tag="AWJ20_2271"
/db_xref="GeneID:30034164"
mRNA complement(<853112..>854551)
/gene="RPN4"
/locus_tag="AWJ20_2271"
/product="stress-regulated transcription factor RPN4"
/transcript_id="XM_018879206.1"
/db_xref="GeneID:30034164"
CDS complement(853112..854551)
/gene="RPN4"
/locus_tag="AWJ20_2271"
/codon_start=1
/product="stress-regulated transcription factor RPN4"
/protein_id="XP_018737143.1"
/db_xref="GeneID:30034164"
/translation="MLIFRNTNSTIVLSDSENSKTPDVQMFPSMTSFGRNQTAGPVFA
AGPSSGYPTTPFKMSSPYYQAELESQRQQLVLPDSHEFVNDLGSSDEQLRFHALSQLN
SNYVPPLSSIAPQSMMKSADEFRYERSMPSLYGQKTEGFDEYFSGGVYINPNERPTME
GGVSDAMDLLDTPEAMDEDDEEEDDDDDEENMFYVEDDDEDLYNDNYYGESAAPGLDH
SPYDATNLLISNPVGPSMPLAMRFQPISEPVQEQVGVLTVNPADLTKMPAQSSEGDLT
NPTIDSYDEFLGSSSASSPSSLASLQSSFSSSDEDYDSIYEPRRLDKIIPEPLRVESI
ISHENHNHNLDHSHNHHHQDDGRSFNSHVQPVEREQPHGQPQHIMTSSVSPSELNNVS
RHEMTKTRRHSAKKNNDLTPPFRCNLVSSESGKECGKVFSRSYDLIRHKDTIHASVRK
TFKCDQCGEASRTFSRMDALTRHMRVKHA"
gene <857137..>859443
/gene="RAD3"
/locus_tag="AWJ20_2272"
/db_xref="GeneID:30034165"
mRNA <857137..>859443
/gene="RAD3"
/locus_tag="AWJ20_2272"
/product="TFIIH/NER complex ATP-dependent 5'-3' DNA
helicase subunit RAD3"
/transcript_id="XM_018879207.1"
/db_xref="GeneID:30034165"
CDS 857137..859443
/gene="RAD3"
/locus_tag="AWJ20_2272"
/inference="similar to AA sequence:KEGG_Orthology:K10844"
/note="5' to 3' DNA helicase; involved in nucleotide
excision repair and transcription; subunit of RNA polII
initiation factor TFIIH and of Nucleotide Excision Repair
Factor 3 (NEF3); homolog of human XPD protein; mutant has
aneuploidy tolerance; protein abundance increases in
response to DNA replication stress; GO_component:
GO:0000439 - core TFIIH complex [Evidence IDA] [PMID
14500720]; GO_component: GO:0000439 - core TFIIH complex
[Evidence IDA] [PMID 19818408]; GO_component: GO:0005675 -
holo TFIIH complex [Evidence IDA] [PMID 19818408];
GO_component: GO:0000112 - nucleotide-excision repair
factor 3 complex [Evidence IDA] [PMID 8855246];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA,IEA];
GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
[Evidence IEA]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0043141 -
ATP-dependent 5'-3' DNA helicase activity [Evidence IDA]
[PMID 2827162]; GO_function: GO:0004003 - ATP-dependent
DNA helicase activity [Evidence IEA]; GO_function:
GO:0008026 - ATP-dependent helicase activity [Evidence
IEA]; GO_function: GO:0003677 - DNA binding [Evidence
IEA,IEA]; GO_function: GO:0000990 - core RNA polymerase
binding transcription factor activity [Evidence IC] [PMID
19818408]; GO_function: GO:0003684 - damaged DNA binding
[Evidence IDA] [PMID 8132553]; GO_function: GO:0004386 -
helicase activity [Evidence IEA]; GO_function: GO:0016787
- hydrolase activity [Evidence IEA]; GO_function:
GO:0016817 - hydrolase activity, acting on acid anhydrides
[Evidence IEA]; GO_function: GO:0016818 - hydrolase
activity, acting on acid anhydrides, in
phosphorus-containing anhydrides [Evidence IEA];
GO_function: GO:0051536 - iron-sulfur cluster binding
[Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0003676 - nucleic
acid binding [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA]; GO_process: GO:0006200
- ATP catabolic process [Evidence IEA]; GO_process:
GO:0032508 - DNA duplex unwinding [Evidence IEA];
GO_process: GO:0006281 - DNA repair [Evidence IEA];
GO_process: GO:0006974 - cellular response to DNA damage
stimulus [Evidence IEA]; GO_process: GO:0006139 -
nucleobase-containing compound metabolic process [Evidence
IEA]; GO_process: GO:0006289 - nucleotide-excision repair
[Evidence IEA]; GO_process: GO:0033683 -
nucleotide-excision repair, DNA incision [Evidence IDA]
[PMID 8631896]; GO_process: GO:0070816 - phosphorylation
of RNA polymerase II C-terminal domain [Evidence IDA]
[PMID 19450536]; GO_process: GO:0070816 - phosphorylation
of RNA polymerase II C-terminal domain [Evidence IDA]
[PMID 19679665]; GO_process: GO:0045951 - positive
regulation of mitotic recombination [Evidence IMP] [PMID
24189665]; GO_process: GO:0000019 - regulation of mitotic
recombination [Evidence IMP] [PMID 10713167]; GO_process:
GO:0000019 - regulation of mitotic recombination [Evidence
IMP] [PMID 9799251]; GO_process: GO:0006355 - regulation
of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0010525 - regulation of transposition,
RNA-mediated [Evidence IMP] [PMID 10713167]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IDA] [PMID 19818408]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="TFIIH/NER complex ATP-dependent 5'-3' DNA
helicase subunit RAD3"
/protein_id="XP_018737144.1"
/db_xref="GeneID:30034165"
/translation="MCDTKKTLDVGGNCILEMPSGTGKTISLLSLTVAYQMHYPEHRK
IVYCSRTMTEIEKALEELKNLMAFRAKELGYVEDFRGLGLSSRKNLCLHPSVKRERKG
AIVDERCRSMTNGISKSKIEAGDTRAQLCEYHENLYELEPHNLIPPGVYTFEDLLKYC
QERRTCPYFTVRRMMSFCNIVIYSYHYLLDPKIAERVSKELSKDCIIIFDEAHNIDNV
CIEALSVDITEDSLRRAAHGAASLGRAVDNVKVNDAEKLQNEYQKLVEGLREAEVARE
EDLFMSNPVLPDDLLSEAVPGNIRRAEHFVAFLKRFIEYLKTRMKVLHVISETPNSFL
KHLKELTYIDKKPLRFCSERLNSLVRTLELTEIEDFTALKEIVTFATLVSTYEKGFIL
ILEPFETEQATVPNPILRFACLDASIAMKPVFDRFSSVIITSGTISPLDMYPRMLNFD
TVIQESYTITLARRSFLPIIVTKGSDQVAISTRFQIRNDPSVVRNYGNLLIEFSKLTP
DGLVVFFPSYLYMESIISMWQTMGILDEVWKYKLILVETPDGQETALALETYRTACSN
GRGAVMFCVARGKVSEGIDFDNQYGRTVLMIGVPFQYTESRILKARLEFLRENFRIKE
NDFLSFDAMRHAAQCLGRVIRGKEDYGIMVLADRRFAKKRTQLPKWINEAILDADVNM
STDMAVAAAKKFLRTMAQPLDPADQKGVSVWDINDLQAHQQELTGVKGKRIEEVTNKP
EHDDQMDVDFDDEGIDDEGIDDSELLNL"
gene <860935..>862557
/gene="AMD2"
/locus_tag="AWJ20_2273"
/db_xref="GeneID:30034166"
mRNA <860935..>862557
/gene="AMD2"
/locus_tag="AWJ20_2273"
/product="Amd2p"
/transcript_id="XM_018879208.1"
/db_xref="GeneID:30034166"
CDS 860935..862557
/gene="AMD2"
/locus_tag="AWJ20_2273"
/inference="similar to AA sequence:KEGG_Orthology:K01426"
/note="Putative amidase; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_function: GO:0004040
- amidase activity [Evidence IEA]; GO_function: GO:0004040
- amidase activity [Evidence ISS] [PMID 2263500];
GO_function: GO:0016884 - carbon-nitrogen ligase activity,
with glutamine as amido-N-donor [Evidence IEA];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016811 - hydrolase activity, acting
on carbon-nitrogen (but not peptide) bonds, in linear
amides [Evidence IEA]; GO_process: GO:0008150 -
biological_process [Evidence ND]; GO_process: GO:0008152 -
metabolic process [Evidence IEA,IEA,IEA]"
/codon_start=1
/product="Amd2p"
/protein_id="XP_018737145.1"
/db_xref="GeneID:30034166"
/translation="MTVSEISLEKLIDEARKRRESQLPDKYRLKKDQLPHHSVKDITG
FFQQSGLFTKHELTITESPVEIILKAIKDQDWTSVEVAEAFCKRAAYTHQITNCLTEM
FFDRAVERAKYLDDYYAQHGKLFGPLHGLPVSIKDHHQIKGTTVTIGFTAWSTTVSSE
DSAIITMLYELGAVLYCKTTVPVAMMMPDTESHLFGRTLNPNNLDHSPGGSSGGEGAL
LACCGAPIGLGSDIGGSIRLPAHSNGIYGLKPTASRFPIAGTRSGLRGQHTIKSVVGP
LSRSLESLEYFTKLIFNSNPENYDPTANPIPWREPIIPEKLVFGVIRTDNYVEPTPAV
KRAVERTVAALESQGHEVIEWEPYMHARMDDILDKTFVADGGKFVKSSRQGEPLFPYM
EPYGKVQESGCSLLWELNYERSEIEMEYFKRWQDSGKLSKSGRRMDGIIVPASPVSSH
PPHHFSYVGYTSVFNAIDYPAVVYPVLKSDSSIDGKPAHEPLSEKDRKVWEAYNPEEY
DNGCVGLQIVCRRHEDEKALALAKVIRDALQQ"
gene complement(<862786..>864387)
/gene="HOL1"
/locus_tag="AWJ20_2274"
/db_xref="GeneID:30034167"
mRNA complement(<862786..>864387)
/gene="HOL1"
/locus_tag="AWJ20_2274"
/product="Hol1p"
/transcript_id="XM_018879209.1"
/db_xref="GeneID:30034167"
CDS complement(862786..864387)
/gene="HOL1"
/locus_tag="AWJ20_2274"
/note="Putative transporter in the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; mutations in membrane-spanning domains permit
cation and histidinol uptake; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0016020 -
membrane [Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0005886 - plasma
membrane [Evidence ISS] [PMID 8955402]; GO_function:
GO:0015665 - alcohol transmembrane transporter activity
[Evidence IMP] [PMID 2405251]; GO_function: GO:0022890 -
inorganic cation transmembrane transporter activity
[Evidence IMP] [PMID 8955402]; GO_process: GO:0006812 -
cation transport [Evidence IMP] [PMID 8955402];
GO_process: GO:0015850 - organic hydroxy compound
transport [Evidence IMP] [PMID 2405251]; GO_process:
GO:0055085 - transmembrane transport [Evidence IEA];
GO_process: GO:0006810 - transport [Evidence IEA]"
/codon_start=1
/product="Hol1p"
/protein_id="XP_018737146.1"
/db_xref="GeneID:30034167"
/translation="MSKSDNIATENSLEVQSSWDAVPGTLNWENKSSNGDLILYPQPS
NDPDDPLNWTKIRKIVNLSLVLFYTFTTGIAGTSTYSILVPISEATGITIAQLNNGTG
YLFLLAGWTNLIWQPMALTFGRRPVFLFSILGCVAMSEWAAHISSYGAWAACRCLLGA
FVAPVEVLPEQCIAELFFSHERSAWMGIYMLVLASSNYLAPLIAGYMNESIGWQWVQH
WCALLLALNFILAFFFYEDTMFNRTTKEAEHVEGSDEDKYGHMITVNSPRNLDYSHKM
NYLQKMKLWRVNPLVTKRGFFDMVWRPVYFFFRFPIVAWAGVFYGFGLVWYNVFNATS
SAILSAPPYNFSSDGVGLSYLAPLIGAIIGGLYCWSIVNYYAISLARKNNGIREPEQR
LWGLVLYNILLPSGLILWGVGAAHEVHWIGLLFGAAFVSICNVLAGSFSLSYLIDCYK
DISGEALVSVILCRNTLSFGINYAITPWINHSGLQNTFIVAAVLGLVTGLTFLIMIFG
GKALRKKGASAYIKAAETQIIHVAH"
gene <867187..>867699
/locus_tag="AWJ20_2275"
/db_xref="GeneID:30034168"
mRNA <867187..>867699
/locus_tag="AWJ20_2275"
/product="hypothetical protein"
/transcript_id="XM_018879210.1"
/db_xref="GeneID:30034168"
CDS 867187..867699
/locus_tag="AWJ20_2275"
/inference="similar to AA sequence:KEGG_Orthology:K03952"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737147.1"
/db_xref="GeneID:30034168"
/translation="MSTTREPNSDYAAYVDQTPMPSHIPDVPEIGATSAPLKSASFFI
GARCLPYNDDYIMCKQESKGNGELDCLKEGRRVTRCAISVLEDINKHCLEEFRLHWQC
LEQNNQQFWGCRPAERLLNKCVFEKLQLEKKIPQVSSDNQIHLKKNPLLKPNTEDYAS
VAAAKRGGVL"
gene complement(<867921..>870398)
/gene="CTF4"
/locus_tag="AWJ20_2276"
/db_xref="GeneID:30034169"
mRNA complement(<867921..>870398)
/gene="CTF4"
/locus_tag="AWJ20_2276"
/product="chromatin-binding protein CTF4"
/transcript_id="XM_018879211.1"
/db_xref="GeneID:30034169"
CDS complement(867921..870398)
/gene="CTF4"
/locus_tag="AWJ20_2276"
/inference="similar to AA sequence:KEGG_Orthology:K11274"
/codon_start=1
/product="chromatin-binding protein CTF4"
/protein_id="XP_018737148.1"
/db_xref="GeneID:30034169"
/translation="MKIKLNPRQAHTAGIVRVCYSLDGNYLISGGANDLVRKFTTGSD
SSEPDSLDHHHDAVLAVDVSDAHFASSSEDGTVHLFDVVSNKPKGVIVRNPLAIREVK
FSPDGKWIATSGNDKFVKIISVTDILKVQEMSLPVPVKHISYSISGNLIALSSIDGNL
RFYRLEKSENKDGSDEPDGDMENSFRQNTIIPPFNPTLVHSLEGIVPDVRDVDDVRTT
AVEFHPDGTHFAIATKTFEVGVYNLSNVSEVARLRHTNGHKKELTGIKWSPNGKYIAT
ISLDNKVIIWDATTFTPVESQNVESPISVAWHPTTNELTVATDRGQLITYDVLLDQAS
PFGDITPFNTTTSTNGTDSSTKKSVNSVDRIRDYDDYDLELEFTGDATADDVEQANGE
GNELDWIEDDDGAGYVGEENGKRSRESTALENGNGYKRAKFVSTEPVHEPFITGSTPW
KNGRRYLCMNTIGYIWSVFQEDHNTVTVTFFDRGLHREYHFTDYVQYDLACLTEEAAL
FANTKTRKALLRFHDGFTNNWEYTLSDNDLFRCISLSKNIVTFCTAQGYVRTFNIYGS
PLSVYRQSRDPVITCASWRNYNYIVRTSPTGSLSYSIENVRTNVIYQKNDEIDIGVGN
KLKAVFFTSQGDPCIFDSKGILLVLQNWREPLQTRWVPIVDSNIESAQGRKESYWPIG
VIDNKFVCTVLRKGDQHPNIPLPATTELELSVTVDPTTTDYEKEYLTKQVLLQLSRAQ
AESDDNIELVSTVSSQELEVDKVVLRLIQACCIDSKPSKALALAYLINKDQALEAASK
IALRFDQSAVAEKINELLNARLRQQQQ"
gene <870960..>871739
/locus_tag="AWJ20_2277"
/db_xref="GeneID:30034170"
mRNA <870960..>871739
/locus_tag="AWJ20_2277"
/product="hypothetical protein"
/transcript_id="XM_018879212.1"
/db_xref="GeneID:30034170"
CDS 870960..871739
/locus_tag="AWJ20_2277"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737149.1"
/db_xref="GeneID:30034170"
/translation="MEHYFDDEDELPSIVQISRKDFSEDEFVVDEFLTKFHKYQTLED
LENQLKSWDKELGHELIDVINKDYGDFVGLGNSLSGGENKIQDVKMNLLRFQTETKKV
KTELENVVEKTNKLIQDKKDLVWLQDFSREMILYLKKLEDIEKSVHDKTESDLEQISE
FAKQYIHLKLLARDLGVKAVQRDALTESRANKLKHSGTHNDDNVNHSIPPILEQKRPR
LARVKSTIIELVDTSIKKKSGTGEEQLRLAILKAKLLETYD"
gene complement(<871938..>873827)
/locus_tag="AWJ20_2278"
/db_xref="GeneID:30034171"
mRNA complement(<871938..>873827)
/locus_tag="AWJ20_2278"
/product="hypothetical protein"
/transcript_id="XM_018879213.1"
/db_xref="GeneID:30034171"
CDS complement(871938..873827)
/locus_tag="AWJ20_2278"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737150.1"
/db_xref="GeneID:30034171"
/translation="MSISRVVSCIITVILLLVAGVAGDDPQATSEPAPALASATAATS
SETGDPQPVGDVNATGLVVPTNQTSNLLPNFVLEVKTANSGLVLSTELSAMTPLNGTV
SSTNGLSDLCSGELTLMNSTTNLNYNMIPLLSCDDADLVAGYLVNVEGSNSPCAVLYS
LHSQGCEFDSTSTFETQLGTVFSITSAQYSASIISQINRTMGPITASISSKASENNPG
NGSPLTSKSTGSTVAMAVLYSITGVVAATFLFIIVSGAIRVHKHPERYGLVSNTTSYD
SNHGPGGEGFGSMQYSRRAKGLARAVLDSIPIVQFKVGNDASGRTTASPKEESIGIDD
NIGRQYAVDGEKQGRDSYSLDNLKAGNESMVETTNESIDELYTPGKNVVEPPTQTESL
PTGPEGAVGTSAVADVSREPVAAKAVPPVSQSVSEVGTETPAASHVNGTQPMETLVGG
LNSDEPDCPICFESFKDGDILRILPCKHRFHALCVDPWLLNSSALCPLCRVDLSLEEN
EQVSNDPPNGLNASSHHEVEISLFNRLLDIWNAQLLPREARRVALARFHEEAELRRQL
RSRQGNNFEEQNSRRWTNFVNSRRRLFQLRRNRRGASDHEEPAHDNDNNPTSDPSAPP
PSSQT"
gene complement(<875950..>877872)
/locus_tag="AWJ20_2279"
/db_xref="GeneID:30034172"
mRNA complement(<875950..>877872)
/locus_tag="AWJ20_2279"
/product="hypothetical protein"
/transcript_id="XM_018879214.1"
/db_xref="GeneID:30034172"
CDS complement(875950..877872)
/locus_tag="AWJ20_2279"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737151.1"
/db_xref="GeneID:30034172"
/translation="MSNSLPASTSDTSSHPVSISDLQDITSSVPIMTPPSSRKSTSTP
SDPIDKYISAKFKKRTSSIVEWLNKTTSKLGGKYVLVVVAPDDGTDIYFNSPMAKFVD
NFLLEKDIVEYLSKGSDGDIDPDRPRVPSIATSTKPNTSTKCKAKLDLCNHDRVISFL
TQGLVIIQQATCKALAKSWIREIEPKKQTNYPYKLGDMSKPDWWPYGVRHKEPDHLNK
DERVQLLVGLLTHEDISIRRIREAAMPSIRDTDPDKLALLEELYIVADSEKQFLNGNL
DESDQIVEVTDLNYWDRQTKIAIPSSNPTTTVKKEKESGSQLKSSSGLGQGRLVNIAP
KSTSRAVISSPSIAHLKFDAHQHFAAVTSDSNTSISSPRGHSNGANNSSSLRIASLSS
TPSSSLSTTTCSSSSSVGSNSTSSSGSSLSTSASSVSNSSFSANSGLSSSFCSNPIQS
PNSSNSNLSYTSKGTPLTAAHYLSQFDTWPGATPLSIPVAGITNRMMFLEHASIPSSS
PFHDVNISHGFYMANRTPHKPIRTFANQELDETPSRTTSGQKFDDIGTSSSSIEHTPN
PFNQLPLQQLPLPFPLPLQIQSENLNVLGKRPMADRFIYLDDDNTHDENRFNKKFDNG
NTKVFYQEETVLKFES"
gene <880201..>881082
/gene="TMT1"
/locus_tag="AWJ20_2280"
/db_xref="GeneID:30034174"
mRNA <880201..>881082
/gene="TMT1"
/locus_tag="AWJ20_2280"
/product="Tmt1p"
/transcript_id="XM_018879216.1"
/db_xref="GeneID:30034174"
CDS 880201..881082
/gene="TMT1"
/locus_tag="AWJ20_2280"
/note="Trans-aconitate methyltransferase; cytosolic enzyme
that catalyzes the methyl esterification of
3-isopropylmalate, an intermediate of the leucine
biosynthetic pathway, and trans-aconitate, which inhibits
the citric acid cycle; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005829 -
cytosol [Evidence IDA] [PMID 11695919]; GO_function:
GO:0008168 - methyltransferase activity [Evidence IEA];
GO_function: GO:0046547 - trans-aconitate
3-methyltransferase activity [Evidence IEA]; GO_function:
GO:0046547 - trans-aconitate 3-methyltransferase activity
[Evidence IDA] [PMID 11695919]; GO_function: GO:0016740 -
transferase activity [Evidence IEA]; GO_process:
GO:0008150 - biological_process [Evidence ND]; GO_process:
GO:0032259 - methylation [Evidence IEA]"
/codon_start=1
/product="Tmt1p"
/protein_id="XP_018737152.1"
/db_xref="GeneID:30034174"
/translation="MTTFAKATFNAASYLSARPHYPDSLFKHIFSYFQKHNSAIKGTP
TKCVDIGCGPGEATLKLSEYFDSAIGIDPGAGMIESATKSARSAGKSSDQLNYIIGSD
KTFTDIIPESSVNIVTAAQASHWFDFPKFIESSAKVLKPNGVLAVWGYVDHVFVNHPK
ASEISLEYSYDKKYMGPYWEQPGRGRLASMLSEAEGQLRANDKFSDVEVIRDESITLN
PGSFYEMTRTLPLRSVHAYMKTFSAYHEWKAQHPDEPDVVDLCFEAIKKAENWTDDTE
VTIKWATVLIMASKKAL"
gene complement(<881240..>882298)
/gene="HST2"
/locus_tag="AWJ20_2281"
/db_xref="GeneID:30034175"
mRNA complement(<881240..>882298)
/gene="HST2"
/locus_tag="AWJ20_2281"
/product="histone deacetylase HST2"
/transcript_id="XM_018879217.1"
/db_xref="GeneID:30034175"
CDS complement(881240..882298)
/gene="HST2"
/locus_tag="AWJ20_2281"
/inference="similar to AA sequence:KEGG_Orthology:K11121"
/note="Cytoplasmic NAD(+)-dependent protein deacetylase;
member of the silencing information regulator 2 (Sir2)
family of NAD(+)-dependent protein deacetylases; modulates
nucleolar (rDNA) and telomeric silencing; possesses
NAD(+)-dependent histone deacetylase activity in vitro;
contains a nuclear export signal (NES); function regulated
by its nuclear export; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 11226170]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 17110954];
GO_function: GO:0051287 - NAD binding [Evidence IEA];
GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
GO_function: GO:0017136 - NAD-dependent histone
deacetylase activity [Evidence IDA] [PMID 10811920];
GO_function: GO:0017136 - NAD-dependent histone
deacetylase activity [Evidence IMP] [PMID 10841563];
GO_function: GO:0016787 - hydrolase activity [Evidence
IEA]; GO_function: GO:0016811 - hydrolase activity, acting
on carbon-nitrogen (but not peptide) bonds, in linear
amides [Evidence IEA]; GO_function: GO:0046872 - metal ion
binding [Evidence IEA]; GO_function: GO:0008270 - zinc ion
binding [Evidence IEA]; GO_process: GO:0000183 - chromatin
silencing at rDNA [Evidence IMP] [PMID 11226170];
GO_process: GO:0000183 - chromatin silencing at rDNA
[Evidence IMP] [PMID 16051752]; GO_process: GO:0001300 -
chronological cell aging [Evidence IGI,IMP] [PMID
16051752]; GO_process: GO:0031939 - negative regulation of
chromatin silencing at telomere [Evidence IMP] [PMID
11226170]; GO_process: GO:0045950 - negative regulation of
mitotic recombination [Evidence IGI,IMP] [PMID 16051752];
GO_process: GO:0006476 - protein deacetylation [Evidence
IEA]; GO_process: GO:0006355 - regulation of
transcription, DNA-templated [Evidence IEA]; GO_process:
GO:0006351 - transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="histone deacetylase HST2"
/protein_id="XP_018737153.1"
/db_xref="GeneID:30034175"
/translation="MSEEDTQLKPTLESVAEQLKEGKFKNVIFMVGAGISTSAGIPDF
RTPGTGLYSNLQRLNLPYPEAVFDISYLRRNPLAFYTLASELYPGKFEPTDFHKFMRT
VDRKGLLRRLYTQNIDTLERIAGVSGEKIIEAHGSFAKNHCIDCKTEMSNEELKRIVW
ESNPGKTTIPKCKKCRGIVKPDIVFFGEGLPRAFFDSMDEDFPESADLVIVAGTSLTV
SPFSQLPEMVDDDCIRILFNMDAVGDLGTRDNDLLILGDCDKNVRKFAELCGWSLDEE
SEEETSEQEIDDSSKETKKQAKEDAEEEAEEMRKEEGREKAELIAEEISKTLEELDLE
EKDANSEKVVEKESDAAE"
gene <883327..>886815
/gene="TAE2"
/locus_tag="AWJ20_2282"
/db_xref="GeneID:30034176"
mRNA <883327..>886815
/gene="TAE2"
/locus_tag="AWJ20_2282"
/product="Tae2p"
/transcript_id="XM_018879218.1"
/db_xref="GeneID:30034176"
CDS 883327..886815
/gene="TAE2"
/locus_tag="AWJ20_2282"
/note="Component of the ribosome quality control complex
(RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a
ribosome-bound complex required for the degradation of
polypeptides arising from stalled translation involved in
protein translation; monitors translation stress and
signals this to Hsf1p; may interact with ribosomes, based
on co-purification experiments; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm;
GO_component: GO:1990112 - RQC complex [Evidence IDA]
[PMID 23178123]; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_component: GO:0010494 -
cytoplasmic stress granule [Evidence IDA] [PMID 23222640];
GO_component: GO:0022625 - cytosolic large ribosomal
subunit [Evidence IDA] [PMID 23479637]; GO_component:
GO:0005840 - ribosome [Evidence IDA] [PMID 16702403];
GO_function: GO:0003729 - mRNA binding [Evidence IDA]
[PMID 23222640]; GO_process: GO:0002181 - cytoplasmic
translation [Evidence IGI,IMP] [PMID 20691087];
GO_process: GO:1990116 - ribosome-associated
ubiquitin-dependent protein catabolic process [Evidence
IGI,IMP] [PMID 23479637]; GO_process: GO:0006412 -
translation [Evidence IEA]"
/codon_start=1
/product="Tae2p"
/protein_id="XP_018737154.1"
/db_xref="GeneID:30034176"
/translation="MRNELESQILNYRLQNIYDLTSSSHLFLLKFAVPDSKKLVIVDP
GFRIHLTEFSRSTTQSPSGFVAKLRKHLKTRRLSRISLAPLNRLLVFSFSDDFSYHLV
LEFFAGGNMILLDKQFKILSLQRTVSATDTQSRCAVGEIYPLDDYLKDDGSADVSFDV
KSVKQWLGGSSNNVPEAIAEDDSQSASTNLLNSDLPKTSTSGPKKKKEAKIALKKLLY
IKVPGIAAGIFEKALLDQSIDPDIKDFGPLLSSEAEITRLLTAVKNASEISKSLTSKS
STPGYIVAKKNAQFQQSTSSDSVDESKFSPETSINNSSIEYLYEEFEPFEPRAFSNPD
IKVFEIETFNQAVDKYFSTLESTKLSLKISNQEQQAEKRLNAARDEKEKRVKGLVQVQ
QKSSTLGYALQAYASRVEEAIEAVQGLLQQGMDWVDIENLIKVEQSRGNPVANTIALP
LNLLKNKITVILPDPDFEQDLEEDDDDKDTSDSESDSDSDSESESESTAKNVKTKKKA
APTVKVEIDITLSAWANSRKYFEVKKTAVAKQERTLQQADQAYKSAEKKIKRDLKMAL
EKEKGQQHTLHAIRETYWFEKFYWFLSSDGYLCIGGRDSFQNELLLRRYFKKNDIYVH
CDVGGASVVIIKNHIADSREVPPATLTQAGVLSVATSKAWDSKMVTSAWWATRDQIPR
LTPEGDIVRSDLIIVTGEGKHFLPPSQLDMGLGFLWMVSEDAAAKYSKRGGKNRRDGE
TTDVTNENENNSDSDSSEEEFPDTQIDSDEEFPDTQIASDDDLEDENDVAEEDNEEEE
ANSELEDILKDESKDALSESEQSDQEADPENEAIADSYDKGDAHNGAGPGEAEADADM
DVGADADADSSDISRGPDSENRSGTTGKRISAAERRRLRKQKLRSDSNTPEPESSTAP
VSSAVSDIEKALSDLQVSKREKEPAAQKPAKVRGKKGKLKKIASKYADQDEEERSRRM
ELLGTTRGLEKAKAQAEQEKAKRQQQEQARRDRQNRAKEQEKKRLLEAEELGDDDEEV
DDDSKYPFDKLVPRLSVGDEPQTIVPVFAPWGALQRYKYKMKFQPGTVKKGKAVKDIL
YSINNAKVDATASDDDYPWPNEIALVRSLKEPELLLAIGVGRVKISIPGSRQKAVKGG
SKGQKGGSKRGKGSKR"
gene complement(<887005..>887598)
/gene="RET3"
/locus_tag="AWJ20_2283"
/db_xref="GeneID:30034177"
mRNA complement(<887005..>887598)
/gene="RET3"
/locus_tag="AWJ20_2283"
/product="Ret3p"
/transcript_id="XM_018879219.1"
/db_xref="GeneID:30034177"
CDS complement(887005..887598)
/gene="RET3"
/locus_tag="AWJ20_2283"
/note="Zeta subunit of the coatomer complex (COPI); COPI
coats Golgi-derived transport vesicles; involved in
retrograde transport between Golgi and ER; GO_component:
GO:0030126 - COPI vesicle coat [Evidence IMP,ISS] [PMID
8617224]; GO_component: GO:0030663 - COPI-coated vesicle
membrane [Evidence IEA]; GO_component: GO:0005794 - Golgi
apparatus [Evidence IEA]; GO_component: GO:0000139 - Golgi
membrane [Evidence IEA]; GO_component: GO:0005737 -
cytoplasm [Evidence IEA,IEA]; GO_component: GO:0031410 -
cytoplasmic vesicle [Evidence IEA]; GO_component:
GO:0016020 - membrane [Evidence IEA]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0015031 - protein transport [Evidence IEA]; GO_process:
GO:0006890 - retrograde vesicle-mediated transport, Golgi
to ER [Evidence IMP] [PMID 8617224]; GO_process:
GO:0006810 - transport [Evidence IEA,IEA]; GO_process:
GO:0016192 - vesicle-mediated transport [Evidence IEA]"
/codon_start=1
/product="Ret3p"
/protein_id="XP_018737155.1"
/db_xref="GeneID:30034177"
/translation="MPSPDISLYSVEAVLILDNDGKRLFAKYYQPPYADSNAAVTDNK
LATSVKDQTAFEKGLFAKTNKQNADVILYDSKVIVYKQIVDISLYIVGGMEENEAMLY
QVVLGLRDALDILLKHSADKRTVLENYDLVSLAVDETIDSGIILEVDPVIIASRVSKA
PTNEPSINNIDLSEQGLLNVYQFARGKLSEKLRQQFQ"
gene <888608..>889438
/gene="TAF3"
/locus_tag="AWJ20_2284"
/db_xref="GeneID:30034178"
mRNA <888608..>889438
/gene="TAF3"
/locus_tag="AWJ20_2284"
/product="Taf3p"
/transcript_id="XM_018879220.1"
/db_xref="GeneID:30034178"
CDS 888608..889438
/gene="TAF3"
/locus_tag="AWJ20_2284"
/inference="similar to AA sequence:KEGG_Orthology:K14650"
/note="TFIID subunit (47 kDa); involved in promoter
binding and RNA polymerase II transcription initiation;
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_component: GO:0005669 - transcription factor TFIID
complex [Evidence IDA] [PMID 10788514]; GO_component:
GO:0005669 - transcription factor TFIID complex [Evidence
IDA] [PMID 15448131]; GO_function: GO:0001075 - RNA
polymerase II core promoter sequence-specific DNA binding
transcription factor activity involved in preinitiation
complex assembly [Evidence IC] [PMID 15448131];
GO_function: GO:0003682 - chromatin binding [Evidence IDA]
[PMID 17660549]; GO_process: GO:0051123 - RNA polymerase
II transcriptional preinitiation complex assembly
[Evidence IC] [PMID 10788514]; GO_process: GO:0006355 -
regulation of transcription, DNA-templated [Evidence IEA];
GO_process: GO:0006366 - transcription from RNA polymerase
II promoter [Evidence IDA] [PMID 12138208]; GO_process:
GO:0006366 - transcription from RNA polymerase II promoter
[Evidence IDA] [PMID 15448131]; GO_process: GO:0006351 -
transcription, DNA-templated [Evidence IEA]"
/codon_start=1
/product="Taf3p"
/protein_id="XP_018737156.1"
/db_xref="GeneID:30034178"
/translation="MSEEFFFSLVRISCAQIFRAAGIERCSPSLLDKASDLLVRHLTA
LSEKASDNAMLSGRNEVVIQDLALAMEELCVIRPRAILDPYDLDPNSFIGYENFIDWV
KGPVPEEARRISRIPRVTPIGQKVENTSSGIISISTDGNTGTTPDGIESKDNSDTRAK
SEKPEDTRGIDGETRVGSTSSKAPVAASTATKTTSTEASTTQTNTESEKTAGKVHPHD
DEEWLTALMKKRIKVGNENKFKGTVLSIEDGLETNPEVKIVGGPVSLDEFQPKNLSVQ
"
gene complement(<889769..>890290)
/gene="LRG1"
/locus_tag="AWJ20_2285"
/db_xref="GeneID:30034179"
mRNA complement(<889769..>890290)
/gene="LRG1"
/locus_tag="AWJ20_2285"
/product="Lrg1p"
/transcript_id="XM_018879221.1"
/db_xref="GeneID:30034179"
CDS complement(889769..890290)
/gene="LRG1"
/locus_tag="AWJ20_2285"
/note="GTPase-activating protein (GAP); contains
Rho1p-specific GAP activity, interacting with activated
forms of Rho1p; functions along with Sac7p as a negative
regulator of the Pkc1p-mediated cell wall integrity
signaling pathway; negative regulator of cell wall
1,3-beta-glucan biosynthesis; required for efficient cell
fusion; contains a RhoGAP domain and three
Lin-11-Isl1-Mec-3 (LIM) domains; GO_component: GO:0005933
- cellular bud [Evidence IEA]; GO_component: GO:0005935 -
cellular bud neck [Evidence IEA]; GO_component: GO:0005935
- cellular bud neck [Evidence IDA] [PMID 11447600];
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005935 - cellular
bud neck [Evidence IDA] [PMID 23613772]; GO_component:
GO:0005934 - cellular bud tip [Evidence IDA] [PMID
11447600]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0000131 - incipient
cellular bud site [Evidence IDA] [PMID 11447600];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 15514049]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0030427 - site of
polarized growth [Evidence IDA] [PMID 23613772];
GO_function: GO:0005096 - GTPase activator activity
[Evidence IEA]; GO_function: GO:0005100 - Rho GTPase
activator activity [Evidence IDA] [PMID 15514049];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0008270 - zinc ion binding [Evidence
IEA]; GO_process: GO:0000755 - cytogamy [Evidence IGI,IMP]
[PMID 15514049]; GO_process: GO:0035024 - negative
regulation of Rho protein signal transduction [Evidence
IGI] [PMID 11447600]; GO_process: GO:0035024 - negative
regulation of Rho protein signal transduction [Evidence
IGI,IMP] [PMID 11713681]; GO_process: GO:0090038 -
negative regulation of protein kinase C signaling
[Evidence IGI,IMP] [PMID 11713681]; GO_process: GO:0032321
- positive regulation of Rho GTPase activity [Evidence
IDA] [PMID 15514049]; GO_process: GO:0090334 - regulation
of cell wall (1->3)-beta-D-glucan biosynthetic process
[Evidence IGI] [PMID 11447600]; GO_process: GO:0060237 -
regulation of fungal-type cell wall organization [Evidence
IGI] [PMID 11447600]; GO_process: GO:0060237 - regulation
of fungal-type cell wall organization [Evidence IGI,IMP]
[PMID 11713681]; GO_process: GO:0007165 - signal
transduction [Evidence IEA]; GO_process: GO:0030435 -
sporulation resulting in formation of a cellular spore
[Evidence IEA]"
/codon_start=1
/product="Lrg1p"
/protein_id="XP_018737157.1"
/db_xref="GeneID:30034179"
/translation="MEVLFYFLNWTASFSHIDEETGSKMDAHNLATVITPNILYIKEG
GHEQGDAYFLSIESVNTLIEEQSRFAEVPVEVQVIYRESGLANASADITSKDVISKVE
ACIKQIGGVDAVVVNNSTSPSSGSTTPQNLEEPHRPLPIRVNTSDIAALPVPGQEASI
KRVNKTEMSPVSP"
gene complement(<890454..>894704)
/gene="LRG1"
/locus_tag="AWJ20_2286"
/db_xref="GeneID:30034180"
mRNA complement(<890454..>894704)
/gene="LRG1"
/locus_tag="AWJ20_2286"
/product="Lrg1p"
/transcript_id="XM_018879222.1"
/db_xref="GeneID:30034180"
CDS complement(890454..894704)
/gene="LRG1"
/locus_tag="AWJ20_2286"
/note="GTPase-activating protein (GAP); contains
Rho1p-specific GAP activity, interacting with activated
forms of Rho1p; functions along with Sac7p as a negative
regulator of the Pkc1p-mediated cell wall integrity
signaling pathway; negative regulator of cell wall
1,3-beta-glucan biosynthesis; required for efficient cell
fusion; contains a RhoGAP domain and three
Lin-11-Isl1-Mec-3 (LIM) domains; GO_component: GO:0005933
- cellular bud [Evidence IEA]; GO_component: GO:0005935 -
cellular bud neck [Evidence IEA]; GO_component: GO:0005935
- cellular bud neck [Evidence IDA] [PMID 11447600];
GO_component: GO:0005935 - cellular bud neck [Evidence
IDA] [PMID 14562095]; GO_component: GO:0005935 - cellular
bud neck [Evidence IDA] [PMID 23613772]; GO_component:
GO:0005934 - cellular bud tip [Evidence IDA] [PMID
11447600]; GO_component: GO:0005737 - cytoplasm [Evidence
IEA,IEA]; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 14562095]; GO_component: GO:0000131 - incipient
cellular bud site [Evidence IDA] [PMID 11447600];
GO_component: GO:0005622 - intracellular [Evidence IEA];
GO_component: GO:0043332 - mating projection tip [Evidence
IDA] [PMID 15514049]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0030427 - site of
polarized growth [Evidence IDA] [PMID 23613772];
GO_function: GO:0005096 - GTPase activator activity
[Evidence IEA]; GO_function: GO:0005100 - Rho GTPase
activator activity [Evidence IDA] [PMID 15514049];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_function: GO:0008270 - zinc ion binding [Evidence
IEA]; GO_process: GO:0000755 - cytogamy [Evidence IGI,IMP]
[PMID 15514049]; GO_process: GO:0035024 - negative
regulation of Rho protein signal transduction [Evidence
IGI] [PMID 11447600]; GO_process: GO:0035024 - negative
regulation of Rho protein signal transduction [Evidence
IGI,IMP] [PMID 11713681]; GO_process: GO:0090038 -
negative regulation of protein kinase C signaling
[Evidence IGI,IMP] [PMID 11713681]; GO_process: GO:0032321
- positive regulation of Rho GTPase activity [Evidence
IDA] [PMID 15514049]; GO_process: GO:0090334 - regulation
of cell wall (1->3)-beta-D-glucan biosynthetic process
[Evidence IGI] [PMID 11447600]; GO_process: GO:0060237 -
regulation of fungal-type cell wall organization [Evidence
IGI] [PMID 11447600]; GO_process: GO:0060237 - regulation
of fungal-type cell wall organization [Evidence IGI,IMP]
[PMID 11713681]; GO_process: GO:0007165 - signal
transduction [Evidence IEA]; GO_process: GO:0030435 -
sporulation resulting in formation of a cellular spore
[Evidence IEA]"
/codon_start=1
/product="Lrg1p"
/protein_id="XP_018737158.1"
/db_xref="GeneID:30034180"
/translation="MSTAGREKTKNPHNLFLNLHNSNKEANGRTREHREHRQNPPPTQ
GQSPQQPHHYQPPVPQQQRSRQQQPQQQIPAPHQRQYHQQLRQFPGQPPLQSTGNNVP
GLSLNSPVHMANSGANRVHKQSASISGYYPVAGDGASPVIGLESPNQFKGQGYVSDSS
GASFGGSTGSNSPRQFSGIDSPTRSIPMTSPRYRNFSRPNTFYQTESSSLGNDVFSRD
PGNQSYFQNLPTPPLGGTNTATETTVSSNSKPTTGSNTRSFSIRKVSQDGGDPSFNEP
VERMPPMPGTNAGPQGQSQSNWSSHLQTNPITTTTNTSNTSMPPPATNSTRNFQNNSN
NTDHVYDSNEPSSSNNSNNNNHNNGALPDTNSNNRVYTTNSVYGANSSLSALNTSGLP
NNNSTSSITSPISPSKPARSKTCRKCGDPISGQFVRALDAAYHIECFTCADCNDQCSS
KFFPTDNPDYDPTAPSNVPAHIPLCETCYFKRLDLLCYACNGALRGSYITALGRKYHV
DHFTCSMCDTVFGPEDSYYEHGDSIYCHFHYSTLYASKCEGCQTAILKQFVEIYRGGR
EQQWHPECYMIFKFWNVKLCPEGSKPTLNPLRPLSVTAAAPLDQLNHLQVAQHEKEVD
ERVYRIWTVLCGYEEATASYISDMLQSASNGKYNEALVATANLVAKVDVLFGALDMLN
LVIDPLIKEQEDRRKREDEEDELAVNNTGAHDENVRRPSVTSIPTFTVLGREPKNLCK
KIVEFMSMVSKSREKNVKKMGVTLLNLVTSLAHYLKVLIRYGLSNSLLYDRSFPNGNA
VDSFLQRVEVHTKSVSVLNNLGVSAMTSDRCVRCESSVEDACVRFGDRLWHVGCFLCD
KCGRNLSGEVDTAAWHVSEKMVICQECGGKHNSDESGKLGFVYVTRLRQFVFLVKIAV
ARLQLVLSAYEARDKKAKRISGTGDSMSTPALAHYKASTSASDRSNQSAGSSSTAVNE
PTQNEKGYMTTLTDIRRLRSAKLDRQVSEMSRRARQSRILAMPDSESALVKNNNDSSN
SLEGGGSTTMNSPDEHEQQRRPSNYSEDKVLVNERSRPSVSSQQDPFASTATSSTHDA
SSKSQQQTSEGATATTSGSVTTSTNATSGAGSGAGSNKSLLRNAKRTFSIKRRKELKI
EELGYKQDPSRLDRTTDLFKNEKTLTLDDIPRIVAAEQAREQLPNAFRHQARTTSMTN
SVPMPKSIADSGAKDETRTYMSELSKSELFYIRHVCVVLLQPLVSEWFSQDELLDLIE
TRRQQSIWGKFGKAFGGGGGNSNTTAAKKKGTGAVFGTPLEQLVEKYGVDSTLGVGPK
PLRIPAFVDDCISSMRQKDMSVEGIFRKNGNIRRLKEFTEKIDKNPDKSSILSEENAI
QLAALLKKFLRELPEPLMTSKLQKLWIMTQSKYFPLVP"
gene <897720..>900917
/locus_tag="AWJ20_2287"
/db_xref="GeneID:30034181"
mRNA <897720..>900917
/locus_tag="AWJ20_2287"
/product="Protein BRE4"
/transcript_id="XM_018879223.1"
/db_xref="GeneID:30034181"
CDS 897720..900917
/locus_tag="AWJ20_2287"
/codon_start=1
/product="Protein BRE4"
/protein_id="XP_018737159.1"
/db_xref="GeneID:30034181"
/translation="MAVLVSSVITNKDSSKPKGVRRSRSSIENSISERTSLLSSVGGT
VRIDSGAATTTHDVFAETAASNEDNASSLQRLIKTYEDARQFIFSRKGKNILKCSVAY
FIGSLAVYTPIGTWLYGHSDNKHMICTVCVYFHPARTAGSMTEAILFALFALAFGLII
ALSSMIVSALFTDWDLIYVGYVIDLLVFVGGGLGVIAFTKQMVNKPTFNTACSLASIF
LVTILTKEGNVQAGTLSLSKLLQSFMFVLSGVILSTTISYLVWPESAILKLKESLNKS
MDVHSDLLTFIANKFINGQDINTSEFEKLNEELSKTYKSLQTNLHEAKFELLSTGREC
EYEVFQRLIQSSNSLSLHLGGLSNSANIQWHLLNGEESDDESQDESTTNGDTDVTAVR
HRVNGSARPDGRDSDDSSVTSIEDIISRRSVSPPVSGESSSAIAPDSVSAELFSIFTF
HLGPPMISFFSTIKEILDNLPFGNSPNYEVSLHPQYKASLDLATELYSTAREEALAGV
YAHEIFQVERDFDTAADEEAIAASCGNFSYVLEDFGWELSVFITALEDYRNIIENRPR
RSFKWARFWESRSKTFDNSTSSGTANDNTGPDVDGADTERGTDPANPLRRSPTANTHN
VKSFAELDWLIKKSTTEKKQKTSLSLRIWRSMHAFRRTDVQFGIKVGIGAIIFAIPAF
SPSLRPIFSTWRGEWGLITYAIVMSKSVGGTTVTIPIRFLGTLLGAAAAFVAWTLFPE
NQYVLSFLGWVMACLCFYIILFWDTKQAFGRFILLTFNLTVLYSYSLTVYDNDDDDDE
GGAHPIVREIAFHRFVSVFVGSLWAVFIAFIILPNSARKRLRDILSVQWLKMGLVWKA
DCLKTIPRKSAADEPRLVGIQGEADLQSTMVELQTLLGFAPNELRLKGPFPTLQYKNI
LNCTQRILDGFQNIGVLVAKYPKPSSNELELIAYTAADRRELCSRIFLFFYLISSAIK
LGFPLPDKLPSTTHAIDRMLAKMSDYRQRAIAARSDHGDSSTTASKPPPDEEDFVLFY
SYLLVTVSITEELAKIAIQVQDLFGTIEEDMFTVS"
gene <901341..>903587
/gene="ATG2"
/locus_tag="AWJ20_2288"
/db_xref="GeneID:30034182"
mRNA <901341..>903587
/gene="ATG2"
/locus_tag="AWJ20_2288"
/product="Atg2p"
/transcript_id="XM_018879224.1"
/db_xref="GeneID:30034182"
CDS 901341..903587
/gene="ATG2"
/locus_tag="AWJ20_2288"
/note="Peripheral membrane protein required for autophagic
vesicle formation; also required for vesicle formation
during pexophagy and the cytoplasm-to-vaucole targeting
(Cvt) pathway; involved in Atg9p cycling between the
phagophore assembly site and mitochondria; essential for
cell cycle progression from G2/M to G1 under nitrogen
starvation; forms cytoplasmic foci upon DNA replication
stress; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 11382760]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 22842922]; GO_component: GO:0019898 -
extrinsic component of membrane [Evidence IDA] [PMID
11382760]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0000407 - pre-autophagosomal
structure [Evidence IDA] [PMID 11689437]; GO_component:
GO:0000407 - pre-autophagosomal structure [Evidence IDA]
[PMID 18497569]; GO_component: GO:0034045 -
pre-autophagosomal structure membrane [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0032258 - CVT pathway [Evidence IMP]
[PMID 11382761]; GO_process: GO:0000045 - autophagic
vacuole assembly [Evidence IMP] [PMID 11382761];
GO_process: GO:0006914 - autophagy [Evidence IEA,IEA];
GO_process: GO:0006914 - autophagy [Evidence IMP] [PMID
23382696]; GO_process: GO:0044805 - late nucleophagy
[Evidence IMP] [PMID 22768199]; GO_process: GO:0000422 -
mitochondrion degradation [Evidence IMP] [PMID 19793921];
GO_process: GO:0030242 - peroxisome degradation [Evidence
IDA,IEP,IMP] [PMID 11382760]; GO_process: GO:0034727 -
piecemeal microautophagy of nucleus [Evidence IMP] [PMID
18701704]; GO_process: GO:0015031 - protein transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Atg2p"
/protein_id="XP_018737160.1"
/db_xref="GeneID:30034182"
/translation="MAPSWLPQNIQKRIFRYILARLEVFSDLDLKNLDVSVGTKTNLT
LREVQLDTDKFTLPNGVFVRSGKLGTLNLSIGVGNGVEIDGTDVEFTVALSSSSLDED
DLTDLLSRTTADLAVSLLDDNTREKQHGSSAKDSKKSQDTSDSDSDDSGTGVTGYGFG
ELPNMVTKVADTALSQLSIMLHGIKLRLIIDEDCSLEIHVSKVQLSTGKDGLRTIRVS
EIEILTPKIKDTVENNDDDDILNHETDTERGGEDSFHMDEMQNSLMQSTLFSHEEAST
MYMSAMSSMLQSKEQEAQLDRLLWCDFSELSFKGLLRSDFSLNIGTVNISTVPVSHVL
STIVNFVLKFESNTTKAKEDASNGLTHNDHDNGNLIASNEVTDNSQATQFTLKAGVKA
VHISMSSLLPDGLFESEESLTFEVSSINLNIDNKQQLLTVSNIILRQGNEPVVWFAKK
TGDEDEDLVCHLGNKVTVLLPHPASFYFDTDTIVNISEAIKLFSDLQISQPSQSPSSS
LSSLRPKDGKNVTAKFNTIEGQINTGSTTVRATIFPSNFENGKLLIDTVRFSIKHNDS
TYKAGSIEVQDISIVFGGQSLALSTAIFSPDLEQIQAPVNTQLHVSTVMIGISQPVLD
TLISDLKSIHSLVGSALESRSVAQLRQVRIQESELVKFSVVIAKVEVDYSFQSGEESC
VIYTDLGQIELNVLESASTQISIYTVFVTHDFKIKDSSSSYVALLYKANPSEKVCLYV
TIARHQRH"
gene <903791..>906649
/gene="ATG2"
/locus_tag="AWJ20_2289"
/db_xref="GeneID:30034183"
mRNA <903791..>906649
/gene="ATG2"
/locus_tag="AWJ20_2289"
/product="Atg2p"
/transcript_id="XM_018879225.1"
/db_xref="GeneID:30034183"
CDS 903791..906649
/gene="ATG2"
/locus_tag="AWJ20_2289"
/inference="similar to AA sequence:KEGG_Orthology:K17906"
/note="Peripheral membrane protein required for autophagic
vesicle formation; also required for vesicle formation
during pexophagy and the cytoplasm-to-vaucole targeting
(Cvt) pathway; involved in Atg9p cycling between the
phagophore assembly site and mitochondria; essential for
cell cycle progression from G2/M to G1 under nitrogen
starvation; forms cytoplasmic foci upon DNA replication
stress; GO_component: GO:0005737 - cytoplasm [Evidence
IDA] [PMID 11382760]; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 22842922]; GO_component: GO:0019898 -
extrinsic component of membrane [Evidence IDA] [PMID
11382760]; GO_component: GO:0016020 - membrane [Evidence
IEA]; GO_component: GO:0000407 - pre-autophagosomal
structure [Evidence IDA] [PMID 11689437]; GO_component:
GO:0000407 - pre-autophagosomal structure [Evidence IDA]
[PMID 18497569]; GO_component: GO:0034045 -
pre-autophagosomal structure membrane [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0032258 - CVT pathway [Evidence IMP]
[PMID 11382761]; GO_process: GO:0000045 - autophagic
vacuole assembly [Evidence IMP] [PMID 11382761];
GO_process: GO:0006914 - autophagy [Evidence IEA,IEA];
GO_process: GO:0006914 - autophagy [Evidence IMP] [PMID
23382696]; GO_process: GO:0044805 - late nucleophagy
[Evidence IMP] [PMID 22768199]; GO_process: GO:0000422 -
mitochondrion degradation [Evidence IMP] [PMID 19793921];
GO_process: GO:0030242 - peroxisome degradation [Evidence
IDA,IEP,IMP] [PMID 11382760]; GO_process: GO:0034727 -
piecemeal microautophagy of nucleus [Evidence IMP] [PMID
18701704]; GO_process: GO:0015031 - protein transport
[Evidence IEA]; GO_process: GO:0006810 - transport
[Evidence IEA]"
/codon_start=1
/product="Atg2p"
/protein_id="XP_018737161.1"
/db_xref="GeneID:30034183"
/translation="MQLFLRDCVVELNPLDLPSQGLYIIKDSKVDFALGAESTIDLSV
KRSALLLVDEVSNLETVGIPAVSGVTEFFLSQGYVSIGSISTLKSVIKIPSNRKPRVD
ITIDSVTLNTCADSTQVLIQTLNALKPPVEANDGIRFRTELPNLVDIFSEVDETTFMD
GYTSSTALSKMGDDVINDDIPSNLEFVESYYVDQSTRLKWNSGSSSGSLNNGVSRADM
LLEQDLSVLAGKSKNSSNSNSIEGSISSVSEASPYASSTSSSSSRNGSQSYARLDNFE
QRAYTHDSVLNIEEDHFAKDLPSIYEQPTESGSTSPVSTPRRRRSVTATIPTTEKQEP
LITIKARILSVDWLLFDGYDWSFTRDTINKAVNRVEYAADQFHREANGRGVSASDRFT
PSPRNSGEVGNNEERGRIRNESPEGEVQTVVGDLLFNSIYIGVPAGDDPDMLRGAINR
ELNDDMSEAGSNASTNITSRSSSANRRSVDLGYRRKKKLALKRSKRYKMKISLRDVCA
DVIVLGGVNDQVDLSHYSPDTSGIVLNTIDIRVKDLEIFDNVHTSTWNKFLSYMRDAG
ERESHASMVHILLDTVKPVDYLAVSEIQLKVSVLPLRLIVDQDALDFLTRFFEFKDER
FVVAETNEEIFIQKLDVRSVSVKLDYKPKKVDYMGLKSGRTIEFMNFFTLDGADIVLR
HVVLYGIDGFGKVSDLLNGIWMPDIKQNQLGKFLSGLSSVHSLVRLGSGVRDLFVIPL
QEYKKDGRVVRGLQKGAWFFAKNTTNELAKLGAKLAAGTQGVLESAEQALGGSGASAR
SLGAAYRSPYSDDIVSDDEEPSSPSTDQQHIVSLYANQPGGVSEGLRSAYKSMGKNLT
MARDAVADIGVDAAERGNAQGAAIAVMRAAPVVLIRPMIGATEAVSKTLMGVTNQIEP
DQLQDVQDVSIISGLLNESQNSQILTFRNTNDSIQIRL"
gene complement(<907083..>908549)
/gene="GAP1"
/locus_tag="AWJ20_2290"
/db_xref="GeneID:30034185"
mRNA complement(<907083..>908549)
/gene="GAP1"
/locus_tag="AWJ20_2290"
/product="amino acid permease GAP1"
/transcript_id="XM_018879227.1"
/db_xref="GeneID:30034185"
CDS complement(907083..908549)
/gene="GAP1"
/locus_tag="AWJ20_2290"
/inference="similar to AA sequence:KEGG_Orthology:K16261"
/note="General amino acid permease; Gap1p senses the
presence of amino acid substrates to regulate localization
to the plasma membrane when needed; essential for invasive
growth; GO_component: GO:0030134 - ER to Golgi transport
vesicle [Evidence IDA] [PMID 12499351]; GO_component:
GO:0005768 - endosome [Evidence IDA] [PMID 15039776];
GO_component: GO:0000328 - fungal-type vacuole lumen
[Evidence IDA] [PMID 15039776]; GO_component: GO:0016021 -
integral component of membrane [Evidence IEA,IEA];
GO_component: GO:0016021 - integral component of membrane
[Evidence ISM] [PMID 12192589]; GO_component: GO:0005887 -
integral component of plasma membrane [Evidence IDA] [PMID
9199164]; GO_component: GO:0016020 - membrane [Evidence
IEA,IEA,IEA]; GO_component: GO:0005771 - multivesicular
body [Evidence IMP] [PMID 14523026]; GO_component:
GO:0005886 - plasma membrane [Evidence IDA] [PMID
15623581]; GO_component: GO:0005886 - plasma membrane
[Evidence IDA] [PMID 15707981]; GO_function: GO:0015193 -
L-proline transmembrane transporter activity [Evidence
IGI] [PMID 14968425]; GO_function: GO:0015171 - amino acid
transmembrane transporter activity [Evidence IEA];
GO_function: GO:0015171 - amino acid transmembrane
transporter activity [Evidence IMP] [PMID 6759873];
GO_function: GO:0015203 - polyamine transmembrane
transporter activity [Evidence IMP] [PMID 15707981];
GO_process: GO:0003333 - amino acid transmembrane
transport [Evidence IEA]; GO_process: GO:0006865 - amino
acid transport [Evidence IEA,IEA]; GO_process: GO:0006865
- amino acid transport [Evidence IMP] [PMID 6759873];
GO_process: GO:0015846 - polyamine transport [Evidence
IMP] [PMID 15707981]; GO_process: GO:0055085 -
transmembrane transport [Evidence IEA]; GO_process:
GO:0006810 - transport [Evidence IEA,IEA]"
/codon_start=1
/product="amino acid permease GAP1"
/protein_id="XP_018737162.1"
/db_xref="GeneID:30034185"
/translation="MERSLDIESSPKLVESSTPSEGSDRGVVLDKKFGFMTQLIDSFR
PMDVSEINTSEMTLAERSAAITAKSPLKRGLKSRHLQMIAIGGTIGTGLFVGSGGSLS
QGGPASLVIGYILTGMMLFCTLHAVGELAVRFPVAGGFNTYATRFLDPAWGFAMGWIY
ALQWLILLPLELVAASLTIQYWRTGGGAAASVNPDAWVAVFFALIIFINVFSVRAYGE
TEFIFSTIKVVAVVGFIILGIVLVAGGSPNHIGYIGAKYWINPGPFANGFKGVVSVFV
NAAFAYSGSELVGLAAAETQNPHKALPSACKQVFWRITLFYVVALTLVGFLVPYTDPR
LLGTSSVDANASPFVIAIINSGIKSLPSVVNAVILISAVSVGSSSVYACSRCCQALAS
QGFLPKIFGYIDREGRPMVAIVFTLVFGLLSFLAGSSAEATVFDWLLSFSGMASLFTW
GSICLCHIRFRKAMAVQGISLDELTFKSAVGVWGSWFG"
gene <909885..>911462
/locus_tag="AWJ20_2291"
/db_xref="GeneID:30034186"
mRNA <909885..>911462
/locus_tag="AWJ20_2291"
/product="hypothetical protein"
/transcript_id="XM_018879228.1"
/db_xref="GeneID:30034186"
CDS 909885..911462
/locus_tag="AWJ20_2291"
/inference="similar to AA sequence:KEGG_Orthology:K15627"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737163.1"
/db_xref="GeneID:30034186"
/translation="MSVFRRNSKALNESLPFRLSNLVPNSKLELLAKRTNGKQVTANV
KIKINVVDLPPSFSESLVGEFPNTTTIWQILKHFEKKSGINFTRRSVAVPSSGTTSGK
IMYEIASVHLFSRSISKLEELNQSLASLGFNSGNSSLKIRFTITDIEYEQALDILNGF
PDVGVTAGEILAEEDAKVSESRDHSTVPETISSNIPLSADLETHPTNMTSHSVSAPVP
NSSSQFETVTRSKPVSAPPVEVGSETFSKDLPKGGAESTTPLNSEQIEDPNLLSDPRH
VSSQQEQISGPKPVDSSMEISEPELSQGNSVPLPSTTVPTLPNIQVYLPGSDTSSARF
VSSAEEETKMTQQQFVHYHNQIKAMSEGASNRPLMTKALRESEEAKKLSKIKNYQVRV
RLPDQTQIESTFYPDNKASSIYDFVRSVLKEDITTPFALFTSPPKVTLDPQMSLLWDY
KFSQRNLVYLEWNGPLTNGGSTALLKDKYLSTAKPISEDPGIKQDQLDKENQATAPAK
PRGGLSGTPRWLKMGRK"
gene complement(<911524..>913821)
/gene="SOV1"
/locus_tag="AWJ20_2292"
/db_xref="GeneID:30034187"
mRNA complement(<911524..>913821)
/gene="SOV1"
/locus_tag="AWJ20_2292"
/product="Sov1p"
/transcript_id="XM_018879229.1"
/db_xref="GeneID:30034187"
CDS complement(911524..913821)
/gene="SOV1"
/locus_tag="AWJ20_2292"
/note="Mitochondrial hypothetical protein; GO_component:
GO:0005739 - mitochondrion [Evidence IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 14562095]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0003674 - molecular_function [Evidence ND]; GO_process:
GO:0008150 - biological_process [Evidence ND]"
/codon_start=1
/product="Sov1p"
/protein_id="XP_018737164.1"
/db_xref="GeneID:30034187"
/translation="MFKQSSPRLARHLTRRSLLKRGAPLARTERRRLRASEREPSTVQ
PADGSFNIDQLLEALPKLPAHQQDVVRSLLNNQGPAKVDKLLKESYHMVGSKQGPTEQ
RKISAGGRINLRNKLIYDPETSLETLQEYFPLVLQNPTAFDKLFWKSVVQFYKITRAQ
GAKVSEESPPPYQFFGELFEVAKLQTNTRIRYSCIKIVGDILYSYNTVRMDPFNEVEY
MDSLSYFGNSYRAIQIWKSRRTKKDVEGSLYWLEVGILYHQQAKLLAKAEGLAEEMIQ
EFGYMQPRVIIGFINSYGPTGNSQLIDKWVNQLTRVIELNNFKLANEKLSEEAEDRID
AERASALFNQIKAPSASDLEKVVALLLKNQLWSQTAVLIEYMEKCGMKNSAHRMLQVL
DSTTRRLVNEVNTPKTLIPKQFRRSRRLAAPETNRQLMVVISKIISVYPEVLEYPKFY
EAWLRGLTGLELYDNAITVLEAMIDRGIKPTPQHIMSLTRSLLGKNKIEIALELLARL
EESSSEFSPAPTSSLYGLFISYGARRSDHELVSGIITRMYSQGLDQSPQTFNALLFYY
YRHKDFDSLFKLLERVKGLKGFDLSHANYRSIWMILRDYYNLFSLKALAVNGQTMPPN
IRELFIQMVQSGNFRLSMNVFEYVLQTFLLAGDYEGAVAILCFMDTITNIKIPGVITV
SVLQLASKIHQKTTYAFSRPSRNELIVEPGYEAETAFSWEMVANKVCETLEIDGQSTI
SAAKLFVEKIEAPNEYNRLSAADGS"
gene complement(<914026..>918042)
/gene="YCF1"
/locus_tag="AWJ20_2293"
/db_xref="GeneID:30034188"
mRNA complement(<914026..>918042)
/gene="YCF1"
/locus_tag="AWJ20_2293"
/product="ATP-binding cassette glutathione S-conjugate
transporter YCF1"
/transcript_id="XM_018879230.1"
/db_xref="GeneID:30034188"
CDS complement(914026..918042)
/gene="YCF1"
/locus_tag="AWJ20_2293"
/note="Vacuolar glutathione S-conjugate transporter;
member of ATP-binding cassette family; ATPase activity
required to support vacuole fusion; forms stable complexes
with vacuole fusion machinery; regulates Vam7p recruitment
to vacuoles; has role in detoxifying metals such as
cadmium, mercury, and arsenite; also transports
unconjugated bilirubin, selenodigluthatione, and oxidized
glutathione; similar to human cystic fibrosis protein
CFTR; GO_component: GO:0000329 - fungal-type vacuole
membrane [Evidence IDA] [PMID 23658021]; GO_component:
GO:0000329 - fungal-type vacuole membrane [Evidence IDA]
[PMID 8692889]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA,IEA]; GO_component:
GO:0016021 - integral component of membrane [Evidence ISM]
[PMID 12192589]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005774 - vacuolar
membrane [Evidence IEA]; GO_component: GO:0005773 -
vacuole [Evidence IEA]; GO_function: GO:0005524 - ATP
binding [Evidence IEA,IEA]; GO_function: GO:0016887 -
ATPase activity [Evidence IEA]; GO_function: GO:0042626 -
ATPase activity, coupled to transmembrane movement of
substances [Evidence IEA]; GO_function: GO:0015127 -
bilirubin transmembrane transporter activity [Evidence
IGI,IMP] [PMID 10790694]; GO_function: GO:0015431 -
glutathione S-conjugate-exporting ATPase activity
[Evidence IDA] [PMID 10220408]; GO_function: GO:0015431 -
glutathione S-conjugate-exporting ATPase activity
[Evidence IDA] [PMID 12383937]; GO_function: GO:0015431 -
glutathione S-conjugate-exporting ATPase activity
[Evidence IDA] [PMID 12755704]; GO_function: GO:0015431 -
glutathione S-conjugate-exporting ATPase activity
[Evidence IDA] [PMID 9217966]; GO_function: GO:0017111 -
nucleoside-triphosphatase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA,IEA]; GO_process: GO:0006200 - ATP catabolic process
[Evidence IEA]; GO_process: GO:0015723 - bilirubin
transport [Evidence IGI,IMP] [PMID 10790694]; GO_process:
GO:0008152 - metabolic process [Evidence IEA]; GO_process:
GO:0046686 - response to cadmium ion [Evidence IEA];
GO_process: GO:0010038 - response to metal ion [Evidence
IDA] [PMID 10220408]; GO_process: GO:0010038 - response to
metal ion [Evidence IDA] [PMID 12755704]; GO_process:
GO:0010038 - response to metal ion [Evidence IDA] [PMID
9217966]; GO_process: GO:0055085 - transmembrane transport
[Evidence IEA]; GO_process: GO:0055085 - transmembrane
transport [Evidence IMP] [PMID 10790694]; GO_process:
GO:0006810 - transport [Evidence IEA,IEA]; GO_process:
GO:0042144 - vacuole fusion, non-autophagic [Evidence IMP]
[PMID 23658021]"
/codon_start=1
/product="ATP-binding cassette glutathione S-conjugate
transporter YCF1"
/protein_id="XP_018737165.1"
/db_xref="GeneID:30034188"
/translation="MLLWYWLVALTSSSLKLTNSLLDEEYNSSLLLFLLRTFVTVNSV
FVFSLEWLVPRIRNRNVDKDLRNADIFSNLFLLWLNPLINTAFKTDITERDLPEMEDS
FSTSELSDKLLDHWNKSSRKGAVGMAFTIARTLRLELVALIGAGFAIDILTYIRPFLL
QKILLFVREYNNDRSLPLSIGIYWTLLNGLVSLWIVFLQNRIGLSAVRLSVGVNGAIY
GSVFVKALKLSARSRETKTASHIINLMTTDLGQVNNCMQQLLPLFTTPLEIVYCFIVL
YSFLGFSFFGGVSIMIIVVPLNAYVSSKFRQLTVDLMKAIDERVKITTSMFSNAKSLK
LYAWEGPWLDRLKEARNEHELVLRKKVMFWLTILTGIWSIVPYFIAAGVFGAFLYMED
RPLTADIIFPSLSLFNLLSGPLIAIPYGITMLIQSKVSFQRFADLLSADEQPTDQLIH
LPPVNDPKSKLPVVKFSNTTVSWSESEDAAVALDEIDFEAYKRQLLCVIGKVGAGKSA
LVKTLCGELFLKQGSVSIRGNIAYVPQEPWLLNQSVKDNILFRNKYDPEFYQKTLEAC
DLVYDIKNLPDGDETEVGERGISLSGGQKARVSLARAVYARADVYVLDDILSAVDEHV
GKHLIDHVLGKHGLLASKTIVLATNNVKVLAEADNIILIEDKKIRESGSPLETIGKKG
PIYELMKEFGAAQHETVEELKEEIVTVAVQEEPEEQFDDLRRASIESFRSISEEATKE
LERRKKSQDAVESGAVKSSVYLRYFQASGIGRVTLSVIMIIMVGALTTLGSVWLKLWS
DQSITGATSESLYYLGVYVLIGIVYGVIHVSSTYIAQAIAGLKAAEVLHDNMAQMVLR
SPMSFFEQTPLGQILNRFTTDINEIDNSVANTLASTFRVLMTMFFAVLVVCYSTPIVT
IAVLPVTILYWYYQKFYLVASRQLKRLSSAARSPMMANFEESVKGIKVIRAFEKDRHF
IHENNIRSDLVLKTVSHAASVDRWLALRLGIMGSLFVLFTGISGVYMAKLGLMTEGVL
GLGMSNAMIITSQLWMVVRQSIEIERRGVTIERIFQYHALKSEAPEIVENNRPPAYWP
SEGRITFDKFSTRYKEGMKLVLKDINLDIKPREKIGVVGRTGAGKSTLTLSLFRLIEP
ASGTVVVDNINTNDIGLLDLRHNMSIIPQDAQIFSGTVRDNLDPFRERDDTDLWRVLE
LCHLKDHVSGMEGGLDAALADSGENLSKGQAQLICLARALLHDSNILVLDEATASVDV
ETDKIIQQTIRTELKHKTIITIAHRLNTIMDSDRIVVLEKGEIAEFDSPQNLLAKPES
LFYGLCEKAGLVGNEDKN"
gene complement(<925526..>926143)
/gene="SDT1"
/locus_tag="AWJ20_2294"
/db_xref="GeneID:30034189"
mRNA complement(<925526..>926143)
/gene="SDT1"
/locus_tag="AWJ20_2294"
/product="Sdt1p"
/transcript_id="XM_018879231.1"
/db_xref="GeneID:30034189"
CDS complement(925526..926143)
/gene="SDT1"
/locus_tag="AWJ20_2294"
/inference="similar to AA sequence:KEGG_Orthology:K18551"
/note="Pyrimidine nucleotidase; responsible for production
of nicotinamide riboside and nicotinic acid riboside;
overexpression suppresses the 6-AU sensitivity of
transcription elongation factor S-II, as well as
resistance to other pyrimidine derivatives; SDT1 has a
paralog, PHM8, that arose from the whole genome
duplication; GO_component: GO:0005575 - cellular_component
[Evidence ND]; GO_function: GO:0016787 - hydrolase
activity [Evidence IEA]; GO_function: GO:0008252 -
nucleotidase activity [Evidence IDA] [PMID 11934891];
GO_process: GO:0008152 - metabolic process [Evidence IEA];
GO_process: GO:0006206 - pyrimidine nucleobase metabolic
process [Evidence IMP] [PMID 11934891]"
/codon_start=1
/product="Sdt1p"
/protein_id="XP_018737166.1"
/db_xref="GeneID:30034189"
/translation="MQQLIHKYFVTHLELDDESAELLHQRYYKEFGLAIEGLVRFNKI
DAMAYNAEVDDALPLDGIMKPDPDLRKMLLSIDRTKVKKLWLLTNAYINHARRVVRLL
GVDDLFDGITYCDYAQVPLICKPKREMFHKAMEQAGVDDVNKCYFVDDSYINIVGARK
FGFKKPIQYVDADDPLPEKPASDYTVRSLLELPQLVPEVFKIEEN"
gene complement(<927087..>928073)
/gene="IDH2"
/locus_tag="AWJ20_2295"
/db_xref="GeneID:30034190"
mRNA complement(<927087..>928073)
/gene="IDH2"
/locus_tag="AWJ20_2295"
/product="isocitrate dehydrogenase (NAD(+)) IDH2"
/transcript_id="XM_018879232.1"
/db_xref="GeneID:30034190"
CDS complement(927087..928073)
/gene="IDH2"
/locus_tag="AWJ20_2295"
/inference="similar to AA sequence:KEGG_Orthology:K00030"
/note="Subunit of mitochondrial NAD(+)-dependent
isocitrate dehydrogenase; complex catalyzes the oxidation
of isocitrate to alpha-ketoglutarate in the TCA cycle;
phosphorylated; GO_component: GO:0005962 - mitochondrial
isocitrate dehydrogenase complex (NAD+) [Evidence IDA]
[PMID 16884682]; GO_component: GO:0005759 - mitochondrial
matrix [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 11502169];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 11914276]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961]; GO_function:
GO:0051287 - NAD binding [Evidence IEA]; GO_function:
GO:0003723 - RNA binding [Evidence IEA]; GO_function:
GO:0003824 - catalytic activity [Evidence IEA];
GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
activity [Evidence IEA,IEA]; GO_function: GO:0004449 -
isocitrate dehydrogenase (NAD+) activity [Evidence IDA]
[PMID 16884682]; GO_function: GO:0000287 - magnesium ion
binding [Evidence IEA]; GO_function: GO:0046872 - metal
ion binding [Evidence IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_function:
GO:0016616 - oxidoreductase activity, acting on the CH-OH
group of donors, NAD or NADP as acceptor [Evidence IEA];
GO_process: GO:0006537 - glutamate biosynthetic process
[Evidence TAS] [PMID 9175438]; GO_process: GO:0006102 -
isocitrate metabolic process [Evidence IDA] [PMID
16884682]; GO_process: GO:0008152 - metabolic process
[Evidence IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA,IEA];
GO_process: GO:0006099 - tricarboxylic acid cycle
[Evidence IEA,IEA]; GO_process: GO:0006099 - tricarboxylic
acid cycle [Evidence TAS] [PMID 9175438]"
/codon_start=1
/product="isocitrate dehydrogenase (NAD(+)) IDH2"
/protein_id="XP_018737167.1"
/db_xref="GeneID:30034190"
/translation="MIEGDGIGVEISEAVKDIYKAAKVPIDWELADVTPIFVDGKTSI
PPEAIESINKNKVALKGPLATPVGKGHVSLNLTLRRTFNLFANVRPCKSVVGFQTPYE
DVDTVLIRENTEGEYSGIEHVVVPGVVQSIKLITRAASERVARYAFEYARSIGKKKVL
VVHKATVMKYGDGLFLESAQKIAKEFPDIEIHTELIDRTCLNMVTNPAPYKEYVMVMP
NLYGDIMSDLSSGLIGGLGLTPSGNMGDEVSIFEAVHGSAPDIAGKGLANPTALLLSS
VMMLRHMGLNDEAARIEKAVLDTIASGKENITRDLKGTASTKHFTEQVISRL"
gene <927154..>927447
/locus_tag="AWJ20_2296"
/db_xref="GeneID:30034191"
mRNA <927154..>927447
/locus_tag="AWJ20_2296"
/product="hypothetical protein"
/transcript_id="XM_018879233.1"
/db_xref="GeneID:30034191"
CDS 927154..927447
/locus_tag="AWJ20_2296"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737168.1"
/db_xref="GeneID:30034191"
/translation="MFSLPEAMVSRTAFSILAASSLRPMCLNIITDESSRAVGLAKPL
PAISGADPWTASKMETSSPMLPEGVKPRPPIKPEDKSDMISPYKLGMTMTYSL"
gene complement(<933933..>936488)
/gene="SRC1"
/locus_tag="AWJ20_2297"
/db_xref="GeneID:30034192"
mRNA complement(<933933..>936488)
/gene="SRC1"
/locus_tag="AWJ20_2297"
/product="Src1p"
/transcript_id="XM_018879234.1"
/db_xref="GeneID:30034192"
CDS complement(933933..936488)
/gene="SRC1"
/locus_tag="AWJ20_2297"
/note="Inner nuclear membrane protein; highly enriched at
telomeres and subtelomeric regions; functions in
regulation of subtelomeric genes and is linked to TREX
(transcription export) factors; SRC1 produces 2 splice
variant proteins with different functions; alternative
splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has
aneuploidy tolerance; SEC1 has a paralog, HEH2, that arose
from the whole genome duplication; GO_component:
GO:0000781 - chromosome, telomeric region [Evidence IDA]
[PMID 18762579]; GO_component: GO:0016021 - integral
component of membrane [Evidence IEA]; GO_component:
GO:0005639 - integral component of nuclear inner membrane
[Evidence IEA]; GO_component: GO:0016020 - membrane
[Evidence IEA]; GO_component: GO:0005635 - nuclear
envelope [Evidence IDA] [PMID 16929305]; GO_component:
GO:0005637 - nuclear inner membrane [Evidence IEA];
GO_component: GO:0005634 - nucleus [Evidence IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0043007 - maintenance of rDNA
[Evidence IMP] [PMID 18997772]; GO_process: GO:0000070 -
mitotic sister chromatid segregation [Evidence IGI] [PMID
11754482]"
/codon_start=1
/product="Src1p"
/protein_id="XP_018737169.1"
/db_xref="GeneID:30034192"
/translation="MSRQTGRRSVPPDAGEDERYYLRDGFDPASLTITQLRNVMAVHD
VDFPPSGAKKATWVEHFKVHVLPRAAEFLSEYDNISPHGAGIVDAAPPKRSARSRSPA
KRSPSKSERKSAVEPIAEIPAATEVPPVAELTAPVVAEPASPSKSRSRTTRAKSPAKG
TESTSASTKSKPPPSPSRRSRKTDSTTAPATAIEIETVIESTVTSSEPVPNILESAIS
LAPPGTLASAFESTTGDATSTATTKSSTSKASSSPKKTRKTLKQQYVDPSEYELNTQP
KTEKTLNPVSITGTSPRKSIKEEPLVGQGSSDRNVFQKPTPQKASESPFSNKNIFQKG
SPSSPKARKRAHKEVEESTDSPQPSSAKKRAKDTTPKRSAPKDTFSSIVQFDNDVTMA
DDTKSVPYFTPHIPQQPQEVQPQPDLKQPSEFQPVQYSQYVQPLQQEQQQPIPATPGS
VRRFTSFPNTPEDDHSNTQATPSRSPPRAPPATAPHNSVRDHSFPRASSSKRLSFMPD
VKHLRVSDEFSRQLQQEQQEKKEPAFVLPEQLNDNPKQDQESTEVKNEETEQEAEPVT
DGRVGIEVIKKGEVAAEDLHESDLEKYHELEREIEEEDRALKKKLRKQHKKRSKSKAE
STDYVELFFSALAYVFRVTATLSLVFYASWWCVERFRIGYCDVLTSPEPRTISIPSQW
IEKASPYVSADSLYKFENYINQAIEYGTPECVPCPEHAACYPNFRVACEEGYVPVQSL
ISLGGTLPFPPTCVPDMARRNKVAALAKRAVEILRKRNANVKCGITDESTPEISEDKL
RQELLKQTIASLSEEEFNDLWRQALKDIENEKDIIVRQDKLTFGYAVQRVQIA"
gene <937355..>939925
/gene="AMD1"
/locus_tag="AWJ20_2298"
/db_xref="GeneID:30034193"
mRNA <937355..>939925
/gene="AMD1"
/locus_tag="AWJ20_2298"
/product="AMP deaminase"
/transcript_id="XM_018879235.1"
/db_xref="GeneID:30034193"
CDS 937355..939925
/gene="AMD1"
/locus_tag="AWJ20_2298"
/inference="similar to AA sequence:KEGG_Orthology:K01490"
/note="AMP deaminase; tetrameric enzyme that catalyzes the
deamination of AMP to form IMP and ammonia; thought to be
involved in regulation of intracellular purine (adenine,
guanine, and inosine) nucleotide pools; GO_component:
GO:0005737 - cytoplasm [Evidence IDA] [PMID 14562095];
GO_function: GO:0003876 - AMP deaminase activity [Evidence
IEA,IEA]; GO_function: GO:0003876 - AMP deaminase activity
[Evidence IDA,IMP] [PMID 2690949]; GO_function: GO:0019239
- deaminase activity [Evidence IEA]; GO_function:
GO:0016787 - hydrolase activity [Evidence IEA];
GO_function: GO:0046872 - metal ion binding [Evidence
IEA]; GO_process: GO:0006188 - IMP biosynthetic process
[Evidence IEA]; GO_process: GO:0032264 - IMP salvage
[Evidence IEA]; GO_process: GO:0006178 - guanine salvage
[Evidence IGI,IMP] [PMID 19635936]; GO_process: GO:0009117
- nucleotide metabolic process [Evidence IEA]; GO_process:
GO:0006163 - purine nucleotide metabolic process [Evidence
IGI] [PMID 15869715]; GO_process: GO:0006163 - purine
nucleotide metabolic process [Evidence IGI] [PMID
18433446]; GO_process: GO:0006163 - purine nucleotide
metabolic process [Evidence IGI,IMP] [PMID 19635936];
GO_process: GO:0006163 - purine nucleotide metabolic
process [Evidence IMP] [PMID 20087341]; GO_process:
GO:0009168 - purine ribonucleoside monophosphate
biosynthetic process [Evidence IEA]"
/codon_start=1
/product="AMP deaminase"
/protein_id="XP_018737170.1"
/db_xref="GeneID:30034193"
/translation="MSVSEIMFQNDDDRMEADKEIEMEDGFDEQELINMSPSSTTAHS
YVDEKSLQAMAAKLNALNVHGGGNGSSTAGSVNTGFGGSLDGSGSPGSFHGIGSPHES
LHSSPAKSRIASSNTLKSLVDPSLSPKSSHSTTAHAAAAIAAPHPSLDMASQSPHTYG
SFQKQYAQGTFHQEGHKEDPDVFRELNTMYSKVQSCIDTRHKYQSISLQRESDNPKNS
SEWEIYPPPPKPSWKPQTKEFAATVFEEDENERANENDGVEDGEADDHVNEHVTNGTN
PPSRRPRLSRIGSKLEVGDDFDMANCPIPTPSEYIFAADDDSVFQVYESQEALDQGLP
IVDVPTLRDYYMDMDRIISISSDGPVKSFAFRRLQYLEAKWNLYSLLNEYQETADSKK
TPHRDFYNVRKVDTHVHHSACMNQKHLLRFIKYKMKKFPNEVVIYRDNKLLTLTQVFD
SLGLTAYDLSIDTLDMHAHKDSFHRFDKFNLKYNPIGESRLREIFLKTDNYIKGRYLA
EITKEVVEDLEQSKYQMAEYRISIYGRDPNEWSKLAAWVIDNKLLSHNVRWLIQIPRL
YNIYKEGGIVKNFNDVVENVFKPLFEVTKDPSKDPKLHVFLQRVIGFDSVDDESKVDR
RFHKKYPTPKYWDNAQNPPYSYYLYYLYANMSSLNAWRKKLGYNTFVLRPHAGEAGDP
EHLVAAFLTSHSISHGILLRKVPFIQYLYYLDQIGIAMSPLSNNALFLNYDKNPFHSY
FKRGLNVSLSTDDPLQFSYTREPLIEEYSVAAQIYKLSNVDMCELARNSVIQSGFEAA
LKKHWIGKTYTLRGPKGNVMEKTNVPNVRLAYREETFLHEVGLIERYTGISSCLRNER
"
gene complement(<940172..>942076)
/gene="TAH18"
/locus_tag="AWJ20_2299"
/db_xref="GeneID:30034194"
mRNA complement(<940172..>942076)
/gene="TAH18"
/locus_tag="AWJ20_2299"
/product="Tah18p"
/transcript_id="XM_018879236.1"
/db_xref="GeneID:30034194"
CDS complement(940172..942076)
/gene="TAH18"
/locus_tag="AWJ20_2299"
/note="Conserved NAPDH-dependent diflavin reductase;
component of an early step in the cytosolic Fe-S protein
assembly (CIA) machinery; transfers electrons from NADPH
to the Fe-S cluster of Dre2p; plays a pro-death role under
oxidative stress; Tah18p-dependent nitric oxide synthesis
confers high-temperature stress tolerance; possible target
for development of antifungal drugs; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA,IEA];
GO_component: GO:0005739 - mitochondrion [Evidence
IEA,IEA,IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 19194512]; GO_function: GO:0010181 -
FMN binding [Evidence IEA,IEA]; GO_function: GO:0050661 -
NADP binding [Evidence IEA]; GO_function: GO:0003958 -
NADPH-hemoprotein reductase activity [Evidence IEA];
GO_function: GO:0050660 - flavin adenine dinucleotide
binding [Evidence IEA]; GO_function: GO:0005506 - iron ion
binding [Evidence IEA]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA,IEA]; GO_function:
GO:0016651 - oxidoreductase activity, acting on NAD(P)H
[Evidence IEA]; GO_function: GO:0016651 - oxidoreductase
activity, acting on NAD(P)H [Evidence IDA] [PMID
20802492]; GO_process: GO:0016226 - iron-sulfur cluster
assembly [Evidence IEA]; GO_process: GO:0016226 -
iron-sulfur cluster assembly [Evidence IMP] [PMID
20802492]; GO_process: GO:0006809 - nitric oxide
biosynthetic process [Evidence IMP] [PMID 23159617];
GO_process: GO:0055114 - oxidation-reduction process
[Evidence IEA,IEA]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IDA] [PMID
20802492]"
/codon_start=1
/product="Tah18p"
/protein_id="XP_018737171.1"
/db_xref="GeneID:30034194"
/translation="MDSIVGSVSPQRSVLILYGTETGNAQDCAEILAKKCQRLRLNPT
VCGMDDYDVSQLPLESTIIVICSTTGQGDLPRNALKFWRFLLRKKLPPTLLSHIRFTS
FGLGDSSYPRYNWAIRKVHKRLMQLGAREFIGRGEGDEMGTESLDSQFESWIDLISER
LLQPDMFPLAPGTEVISDNILLEPRYKLSVDTKKIRRLTSGEAQTATIALTRGDALVG
TVVSNKRITAQEHFQDVRNFEFTASQEDSTPSLDYLPGDTVGLYPRNDATDVNELLEH
QGWSEIADHPVTVNEEFEKAIIGGLVKPLTLRSLITHHLDISAIPRRSFFAVAWHFST
DNEREQERLKEFSTIEGLQDLYDYANRSRRSILETITEFYSLKIPVEYILDLIPILRP
RLFSISSPSPGSFRAVNDGVQTSDNEKPSDPVNKTSFSLTIAIVKYKTIIKRIRRGVC
TKWVEGLTEGDKIPFSLFRASFKSEDVHDIETPIIMIAPGTGIAPMRSLILTRAAKEA
VLQPDTAASMLLFFGCRYKEKDYLYEDDWNRVSNSGHSSLTVIPAFSRENGGYVQDKL
YSAKDQVSELLVKQNASIYVCGSAGKMPRQVRITIATILEETQNWSTETTEAYVSSME
RTGRYLQETW"
gene <944999..>945778
/gene="MGE1"
/locus_tag="AWJ20_2300"
/db_xref="GeneID:30034197"
mRNA <944999..>945778
/gene="MGE1"
/locus_tag="AWJ20_2300"
/product="Mge1p"
/transcript_id="XM_018879238.1"
/db_xref="GeneID:30034197"
CDS 944999..945778
/gene="MGE1"
/locus_tag="AWJ20_2300"
/inference="similar to AA sequence:KEGG_Orthology:K03687"
/note="N-terminus has a leader sequence with features
reminiscent of mitochondrial import signals. Translated as
a 28KDa precursor protein which is cleaved to a 21KDa
mature form."
/codon_start=1
/product="Mge1p"
/protein_id="XP_018737172.1"
/db_xref="GeneID:30034197"
/translation="MLRRSLMRSSPSSALVSSSVRAATRPVVMARPIARTQMVNSRGL
FGFRSYSETANGEKKDAESAANEASSENTKEETQTSESEYANLSVEELQSKLKELQSK
FEAKDKEVAKFKEHYTRSVADFRNLQETTKREIQKARDYALQKFAKDLLDSVDNFDRA
LAVVPEEKRSDKENHVELANLYEGVKMTQEVFEKTLARHGLVKLNPLGEKFDPNVHEA
TFEVPQPDKEPGTVFHVQQTGFALNDRVLRAPKVGVVKGTD"
gene complement(<946050..>947468)
/gene="MSF1"
/locus_tag="AWJ20_2301"
/db_xref="GeneID:30034198"
mRNA complement(<946050..>947468)
/gene="MSF1"
/locus_tag="AWJ20_2301"
/product="phenylalanine--tRNA ligase"
/transcript_id="XM_018879239.1"
/db_xref="GeneID:30034198"
CDS complement(946050..947468)
/gene="MSF1"
/locus_tag="AWJ20_2301"
/inference="similar to AA sequence:KEGG_Orthology:K01889"
/note="Mitochondrial phenylalanyl-tRNA synthetase; active
as a monomer, unlike the cytoplasmic subunit which is
active as a dimer complexed to a beta subunit dimer;
similar to the alpha subunit of E. coli phenylalanyl-tRNA
synthetase; GO_component: GO:0005737 - cytoplasm [Evidence
IEA]; GO_component: GO:0005759 - mitochondrial matrix
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA]; GO_component: GO:0005739 - mitochondrion
[Evidence IDA] [PMID 14576278]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0005739 - mitochondrion [Evidence IMP]
[PMID 3029120]; GO_function: GO:0005524 - ATP binding
[Evidence IEA,IEA]; GO_function: GO:0004812 -
aminoacyl-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0016874 - ligase activity [Evidence IEA];
GO_function: GO:0000287 - magnesium ion binding [Evidence
IEA]; GO_function: GO:0000166 - nucleotide binding
[Evidence IEA,IEA]; GO_function: GO:0004826 -
phenylalanine-tRNA ligase activity [Evidence IEA,IEA];
GO_function: GO:0004826 - phenylalanine-tRNA ligase
activity [Evidence IDA] [PMID 16162501]; GO_function:
GO:0004826 - phenylalanine-tRNA ligase activity [Evidence
IDA] [PMID 1924298]; GO_function: GO:0004826 -
phenylalanine-tRNA ligase activity [Evidence IMP,ISS]
[PMID 3029120]; GO_function: GO:0000049 - tRNA binding
[Evidence IEA]; GO_process: GO:0070156 - mitochondrial
phenylalanyl-tRNA aminoacylation [Evidence IMP] [PMID
3029120]; GO_process: GO:0006432 - phenylalanyl-tRNA
aminoacylation [Evidence IEA]; GO_process: GO:0006432 -
phenylalanyl-tRNA aminoacylation [Evidence IDA] [PMID
1924298]; GO_process: GO:0043039 - tRNA aminoacylation
[Evidence IEA]; GO_process: GO:0006418 - tRNA
aminoacylation for protein translation [Evidence IEA];
GO_process: GO:0008033 - tRNA processing [Evidence IEA];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="phenylalanine--tRNA ligase"
/protein_id="XP_018737173.1"
/db_xref="GeneID:30034198"
/translation="MTANISRSSGFLLQRVSAPAVRRWNINARSSIAQSLKRNISTES
SSGNATITVLGKEYPTDDFTNLPNSIANLTSRKLHLQPSHPIGILRSLIESRLKDLGF
TSYNDFKPVVSTYENFDALGFPEDHPGRSKTDTYYINEKTLLRTHTSAHESECFQTCE
TPGYLISADVYRRDTIDRTHYPAFHQMEGARVWSRTEHGDNLAKVIQEDLESIPDSGI
IVSDPDPPFHEGNPRQPTMSELETELIGKHLKKTIELLMGHVFHQAREAAIKAGSTDP
DLFSPIKARWIEAYFPWTSPSWEIEIWWKGEWLEMCGCGVVKHEVLSGAGVGDKIGWA
FGIGLERTAMILFGVPDIRLFWSEDERFLSQFKPNTVSTFKPYSKYPGTFRDVAFWCD
MAKEVHINDLMEVVRSIGGDLVENVSLIDEFVHPKTKQKSQCYRINYQSMDRSLTNAE
INDLQDAVVSSLSTQFPIKIRD"
gene <946438..>946851
/locus_tag="AWJ20_2302"
/db_xref="GeneID:30034199"
mRNA <946438..>946851
/locus_tag="AWJ20_2302"
/product="hypothetical protein"
/transcript_id="XM_018879240.1"
/db_xref="GeneID:30034199"
CDS 946438..946851
/locus_tag="AWJ20_2302"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737174.1"
/db_xref="GeneID:30034199"
/translation="MAVRSRPIPKAQPILSPTPAPERTSCFTTPHPHISSHSPFHQIS
ISQDGEVQGKYASIQRALMGEKRSGSVDPALIAASRAWWNTWPMSSSIVFFKCLPISS
VSSSDIVGWRGLPSWNGGSGSETIMPESGMLSRSS"
gene <948033..>948749
/gene="MRPL16"
/locus_tag="AWJ20_2303"
/db_xref="GeneID:30034200"
mRNA <948033..>948749
/gene="MRPL16"
/locus_tag="AWJ20_2303"
/product="mitochondrial 54S ribosomal protein YmL47"
/transcript_id="XM_018879241.1"
/db_xref="GeneID:30034200"
CDS 948033..948749
/gene="MRPL16"
/locus_tag="AWJ20_2303"
/note="Mitochondrial ribosomal protein of the large
subunit; homologous to bacterial L16 ribosomal protein;
synthetically lethal with hac1 mutant suggesting a role in
the maturation of secretory proteins; GO_component:
GO:0005622 - intracellular [Evidence IEA]; GO_component:
GO:0005762 - mitochondrial large ribosomal subunit
[Evidence IDA] [PMID 7478995]; GO_component: GO:0005762 -
mitochondrial large ribosomal subunit [Evidence IDA] [PMID
9151978]; GO_component: GO:0005739 - mitochondrion
[Evidence IEA,IEA]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16823961];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IEA,IEA]; GO_function: GO:0019843 - rRNA binding
[Evidence IEA]; GO_function: GO:0003735 - structural
constituent of ribosome [Evidence IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IDA] [PMID 7478995]; GO_function: GO:0003735 - structural
constituent of ribosome [Evidence IDA] [PMID 9151978];
GO_process: GO:0032543 - mitochondrial translation
[Evidence IMP] [PMID 7478995]; GO_process: GO:0051604 -
protein maturation [Evidence IMP] [PMID 19433630];
GO_process: GO:0006412 - translation [Evidence IEA]"
/codon_start=1
/product="mitochondrial 54S ribosomal protein YmL47"
/protein_id="XP_018737175.1"
/db_xref="GeneID:30034200"
/translation="MFSRVFSALKPSALLAPSSLLTSVGASRTTALTSRFGGSTFQQI
RLKHQYEPRVRIKRKAQKGRVPVRTGGSIKGSTLQFGKFGLRLKSEGVRLKAVQLKEA
DNAIMRALRPLKGKLFRRLTTNIPVCVKGNETRMGKGKGGFEFWACRVPTGKILFEID
GANVHESVARDAFRIAGDKLPGTYEFVNLETAPKAGFQPVNVDGSPVNFYEEAKANPT
KQYANVLQGQDPELRKYLRR"
gene complement(<949020..>950087)
/gene="RFC5"
/locus_tag="AWJ20_2304"
/db_xref="GeneID:30034201"
mRNA complement(<949020..>950087)
/gene="RFC5"
/locus_tag="AWJ20_2304"
/product="replication factor C subunit 5"
/transcript_id="XM_018879242.1"
/db_xref="GeneID:30034201"
CDS complement(949020..950087)
/gene="RFC5"
/locus_tag="AWJ20_2304"
/inference="similar to AA sequence:KEGG_Orthology:K10756"
/note="Subunit of heteropentameric Replication factor C
(RF-C); RF-C is a DNA binding protein and ATPase that acts
as a clamp loader of the proliferating cell nuclear
antigen (PCNA) processivity factor for DNA polymerases
delta and epsilon; GO_component: GO:0031390 - Ctf18
RFC-like complex [Evidence IPI] [PMID 11389843];
GO_component: GO:0005663 - DNA replication factor C
complex [Evidence IDA] [PMID 7651383]; GO_component:
GO:0005663 - DNA replication factor C complex [Evidence
IMP] [PMID 8559655]; GO_component: GO:0031391 - Elg1
RFC-like complex [Evidence IPI] [PMID 12909721];
GO_component: GO:0031391 - Elg1 RFC-like complex [Evidence
IPI] [PMID 12912927]; GO_component: GO:0031389 - Rad17
RFC-like complex [Evidence IDA] [PMID 12604797];
GO_component: GO:0005634 - nucleus [Evidence IEA,IEA];
GO_function: GO:0005524 - ATP binding [Evidence IEA];
GO_function: GO:0003677 - DNA binding [Evidence IEA,IEA];
GO_function: GO:0003689 - DNA clamp loader activity
[Evidence IDA] [PMID 12604797]; GO_function: GO:0003689 -
DNA clamp loader activity [Evidence IDA] [PMID 8995429];
GO_function: GO:0017111 - nucleoside-triphosphatase
activity [Evidence IEA]; GO_function: GO:0000166 -
nucleotide binding [Evidence IEA,IEA]; GO_process:
GO:0006260 - DNA replication [Evidence IEA,IEA];
GO_process: GO:0007049 - cell cycle [Evidence IEA];
GO_process: GO:0006272 - leading strand elongation
[Evidence IDA] [PMID 1346062]; GO_process: GO:0007062 -
sister chromatid cohesion [Evidence IPI] [PMID 11389843];
GO_process: GO:0007062 - sister chromatid cohesion
[Evidence IMP] [PMID 12665596]"
/codon_start=1
/product="replication factor C subunit 5"
/protein_id="XP_018737176.1"
/db_xref="GeneID:30034201"
/translation="MSLWVHKYMPKSLDTLDYHDGLSKSLKSLAASGEFPHMLMYGPS
GAGKKTRVMAVLRELYGPGVSKIKIDGRVFQTNSKKVEFNIVSSVHHLEITPSDMGNN
DRVVIQDLLKEIAQTQQVDLSSKQRFKVVVINEADSLTRDAQSALRRTMEKYSPNLRL
ILLATSTSNIIGPIKSRTLLVRVAAPTNQEIANVLSKVAKKEKVDLPSSPDDLQYLWD
KIARQSQRNLRKSLLILEAMYAQNDKITRDTPVPPADWELVISKLADDTIKKRSVAQL
LQIRGTLYELLTHCIPAPVILKSLTFSLLKKVSPEVGADIIEAAGFYDHRLRQGSKAI
FHLEAYVARVMNLLETFDMTH"
gene complement(<950442..>950783)
/gene="STL1"
/locus_tag="AWJ20_2305"
/db_xref="GeneID:30034202"
mRNA complement(<950442..>950783)
/gene="STL1"
/locus_tag="AWJ20_2305"
/product="glucose-inactivated glycerol proton symporter
STL1"
/transcript_id="XM_018879243.1"
/db_xref="GeneID:30034202"
CDS complement(950442..950783)
/gene="STL1"
/locus_tag="AWJ20_2305"
/codon_start=1
/product="glucose-inactivated glycerol proton symporter
STL1"
/protein_id="XP_018737177.1"
/db_xref="GeneID:30034202"
/translation="MVVMITPVAFANINYYTYTVFAALNLLILVATYIFYPETAGRSL
EEIDKIFEKSNPRTPWDVVKIAQELPHHNAILDEETGYHGEEDVLDKPTHQYQEDTAT
SLSSSGGKGDM"
gene complement(<951331..>951828)
/gene="STL1"
/locus_tag="AWJ20_2306"
/db_xref="GeneID:30034203"
mRNA complement(<951331..>951828)
/gene="STL1"
/locus_tag="AWJ20_2306"
/product="glucose-inactivated glycerol proton symporter
STL1"
/transcript_id="XM_018879244.1"
/db_xref="GeneID:30034203"
CDS complement(951331..951828)
/gene="STL1"
/locus_tag="AWJ20_2306"
/codon_start=1
/product="glucose-inactivated glycerol proton symporter
STL1"
/protein_id="XP_018737178.1"
/db_xref="GeneID:30034203"
/translation="MVEGALITAGIMISYWLDFGFFFAKGSVNWRFPIAFQIVFAIVI
VCFVLVSIHSFCSTYYNSDFLTFQQSLPDSPRWLIKKDRVEEARLIFSALDDVEPDHH
LITAQIEEIVATLIDEERSNAPIRRLFTFGREKHFHRAMLGFWNQAAQQLTGINLVGI
MREEV"
gene complement(<951898..>952176)
/gene="STL1"
/locus_tag="AWJ20_2307"
/db_xref="GeneID:30034204"
mRNA complement(<951898..>952176)
/gene="STL1"
/locus_tag="AWJ20_2307"
/product="glucose-inactivated glycerol proton symporter
STL1"
/transcript_id="XM_018879245.1"
/db_xref="GeneID:30034204"
CDS complement(951898..952176)
/gene="STL1"
/locus_tag="AWJ20_2307"
/codon_start=1
/product="glucose-inactivated glycerol proton symporter
STL1"
/protein_id="XP_018737179.1"
/db_xref="GeneID:30034204"
/translation="MNDSTIQGTVVSLYEIGCLMGALATMRFGDQFGRRKVIFVGAIV
MTIGATLQCTSFSLAQLIVGRIVTGIGNGFITSTVSGTASCYLLLALG"
gene <955858..>956838
/gene="SPS19"
/locus_tag="AWJ20_2308"
/db_xref="GeneID:30034205"
mRNA <955858..>956838
/gene="SPS19"
/locus_tag="AWJ20_2308"
/product="Sps19p"
/transcript_id="XM_018879246.1"
/db_xref="GeneID:30034205"
CDS 955858..956838
/gene="SPS19"
/locus_tag="AWJ20_2308"
/inference="similar to AA sequence:KEGG_Orthology:K13237"
/note="Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary
enzyme of fatty acid beta-oxidation; homodimeric enzyme
required for growth and sporulation on petroselineate
medium; expression induced during late sporulation and in
the presence of oleate; GO_component: GO:0005782 -
peroxisomal matrix [Evidence IDA] [PMID 9268358];
GO_component: GO:0005777 - peroxisome [Evidence IEA,IEA];
GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
(NADPH) activity [Evidence IEA]; GO_function: GO:0008670 -
2,4-dienoyl-CoA reductase (NADPH) activity [Evidence
IDA,ISS] [PMID 9268358]; GO_function: GO:0016491 -
oxidoreductase activity [Evidence IEA]; GO_process:
GO:0030437 - ascospore formation [Evidence IEP,IMP] [PMID
7969036]; GO_process: GO:0009062 - fatty acid catabolic
process [Evidence IDA,ISS] [PMID 9268358]; GO_process:
GO:0055114 - oxidation-reduction process [Evidence IEA];
GO_process: GO:0030435 - sporulation resulting in
formation of a cellular spore [Evidence IEA]"
/codon_start=1
/product="Sps19p"
/protein_id="XP_018737180.1"
/db_xref="GeneID:30034205"
/translation="MSPPEETVTTGNNSSVADGPQKVVLPITPFETYPKARAAPKELI
GVEAALHAAKNPDFQAVSTIAGQFSLKDKVAIVTGGNSGIGLEYSVVLAELGAIVYVL
DLPEAPSEDYAACKAYIKKLATGGSLEYRVANVTDGPGMAKVTHEIAETHGRLDVCIA
NAGILGPVVDCHEYPADWFRKIMEVNVTGVFLTAQAAIKEMLERKIKGSVIFTASMSG
SIINKDMHWIPYTTAKAAVVQLAKAFACEVGTNEIRVNCISPGHIRTRMTEAYLGVEP
LIENCWAAQNPMNRLGSVYELRGTVAYLASDASTYTTGHDLQVCGGHTAW"
gene complement(<957086..>957586)
/locus_tag="AWJ20_2309"
/db_xref="GeneID:30034206"
mRNA complement(<957086..>957586)
/locus_tag="AWJ20_2309"
/product="hypothetical protein"
/transcript_id="XM_018879247.1"
/db_xref="GeneID:30034206"
CDS complement(957086..957586)
/locus_tag="AWJ20_2309"
/inference="similar to AA sequence:KEGG_Orthology:K01808"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737181.1"
/db_xref="GeneID:30034206"
/translation="MARQYKIVVGCDNAGCDYKNAIKADLEKLPSVSQVIDVGVEHSK
DSKAYPSVAIAAAEKVANGEADRAILICGTGLGVAIAANKVKGIRAVTAHDSFSVERS
ILSNNAQILCMGQRVVGIELARRLAKEWMTYEFDTSSASAEKVAEIVNYENANANDAP
SQINGC"
gene <958231..>959997
/gene="DAK1"
/locus_tag="AWJ20_2310"
/db_xref="GeneID:30034208"
mRNA <958231..>959997
/gene="DAK1"
/locus_tag="AWJ20_2310"
/product="Dak1p"
/transcript_id="XM_018879249.1"
/db_xref="GeneID:30034208"
CDS 958231..959997
/gene="DAK1"
/locus_tag="AWJ20_2310"
/inference="similar to AA sequence:KEGG_Orthology:K00863"
/note="Dihydroxyacetone kinase; required for
detoxification of dihydroxyacetone (DHA); involved in
stress adaptation; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 14562095]; GO_function: GO:0005524 -
ATP binding [Evidence IEA,IEA]; GO_function: GO:0004371 -
glycerone kinase activity [Evidence IEA,IEA]; GO_function:
GO:0004371 - glycerone kinase activity [Evidence IDA]
[PMID 12401799]; GO_function: GO:0004371 - glycerone
kinase activity [Evidence ISS] [PMID 9038161];
GO_function: GO:0016301 - kinase activity [Evidence IEA];
GO_function: GO:0000166 - nucleotide binding [Evidence
IEA]; GO_function: GO:0016740 - transferase activity
[Evidence IEA]; GO_function: GO:0050354 - triokinase
activity [Evidence IEA]; GO_process: GO:0019588 -
anaerobic glycerol catabolic process [Evidence IEA];
GO_process: GO:0097237 - cellular response to toxic
substance [Evidence IMP] [PMID 12401799]; GO_process:
GO:0019563 - glycerol catabolic process [Evidence ISS]
[PMID 9038161]; GO_process: GO:0006071 - glycerol
metabolic process [Evidence IEA,IEA]; GO_process:
GO:0016310 - phosphorylation [Evidence IEA]"
/codon_start=1
/product="Dak1p"
/protein_id="XP_018737182.1"
/db_xref="GeneID:30034208"
/translation="MSTKHFFPNQNGLVVRALKGVASQNPSLGVLESDKVLFHLDHDS
SKVSLLSGGGSGHEPAHSGYVGKGMLAAAVCGDVFASPNTKQIMNACKVVGSDDGYIF
IVTNYTGDMLHFGLACEKIKSHGTKVALIKSADDVSVGRTMGGSVGRRGLSGTIVINK
VLGGAAEAGSSFEVVEELGHAIASNIVTIGAGLDHCHVPGNSEEFGGLGPEECEIGLG
IHNEPGVTRLDEIPPIESLVKSLLGYLLDQSDSDRSFVKIESTDKVALMINNLGGLSI
IEQNAIVEVVLDKLSSEYGIVPIRVFAGHFMTSLNAPSFSITLLNITQSVTKGTSESK
IIEYLDAPTDAVNWPSNHYKTSRPIDLKSQVKSLPKSDDNVEKRTLDLLVDPSVLESK
LRTASNNLISAEPDLTKWDTKMGDGDCGKTLESGAMALLRSLDSGLASPGSIIDVLER
IVDITEENMGGTLGAIFGIYFASFSSSLKHIVSTNPNSDALKILAQSANEALDNLRLH
TAAKEGDRTVMDVLIPFCKALVSNYDLKEALEEAHQAAEKTKTLVPKLGRATYVGGVS
EMTVFPPDPGAWALYEILKGIL"
gene <960344..>961111
/gene="TPI1"
/locus_tag="AWJ20_2311"
/db_xref="GeneID:30034209"
mRNA <960344..>961111
/gene="TPI1"
/locus_tag="AWJ20_2311"
/product="triose-phosphate isomerase TPI1"
/transcript_id="XM_018879250.1"
/db_xref="GeneID:30034209"
CDS 960344..961111
/gene="TPI1"
/locus_tag="AWJ20_2311"
/inference="similar to AA sequence:KEGG_Orthology:K01803"
/note="Triose phosphate isomerase, abundant glycolytic
enzyme; mRNA half-life is regulated by iron availability;
transcription is controlled by activators Reb1p, Gcr1p,
and Rap1p through binding sites in the 5' non-coding
region; inhibition of Tpi1p activity by PEP
(phosphoenolpyruvate) stimulates redox metabolism in
respiring cells; E104D mutation in human TPI causes a rare
autosomal disease; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11914276]; GO_component: GO:0005739 -
mitochondrion [Evidence IDA] [PMID 16962558];
GO_component: GO:0005886 - plasma membrane [Evidence IDA]
[PMID 16622836]; GO_function: GO:0003824 - catalytic
activity [Evidence IEA]; GO_function: GO:0016853 -
isomerase activity [Evidence IEA]; GO_function: GO:0004807
- triose-phosphate isomerase activity [Evidence IEA,IEA];
GO_function: GO:0004807 - triose-phosphate isomerase
activity [Evidence IDA] [PMID 236471]; GO_process:
GO:0006094 - gluconeogenesis [Evidence IEA,IEA];
GO_process: GO:0006096 - glycolytic process [Evidence
IEA,IEA,IEA]; GO_process: GO:0006096 - glycolytic process
[Evidence IMP] [PMID 11329176]; GO_process: GO:0008152 -
metabolic process [Evidence IEA]; GO_process: GO:0006098 -
pentose-phosphate shunt [Evidence IEA]"
/codon_start=1
/product="triose-phosphate isomerase TPI1"
/protein_id="XP_018737183.1"
/db_xref="GeneID:30034209"
/translation="MPVIGVSLKTYMSIKDTVSFFEELKSIVATSKDAGVEFFVAPDM
VSLGKIGAYIQENGRRDDYPQLAAQDCFWEDRGAYTGSVSPKSLKELGVQIVEVGHAE
RRAIFGETDEIVAKKAEAAFRNGLVPLLCVGEKVHDTVENAIDACVTQLRAVTNVISP
DCPLLVAYEPVWAIGKEKPADSEYVRGVATGIKKELAGRVGKSRVLYGGSAGPGGLFQ
SLYPCVDGLFLGRFGHKPANVKAVLSEVIQAAQSGAK"
gene complement(<961621..>963468)
/locus_tag="AWJ20_2312"
/db_xref="GeneID:30034210"
mRNA complement(<961621..>963468)
/locus_tag="AWJ20_2312"
/product="hypothetical protein"
/transcript_id="XM_018879251.1"
/db_xref="GeneID:30034210"
CDS complement(961621..963468)
/locus_tag="AWJ20_2312"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737184.1"
/db_xref="GeneID:30034210"
/translation="MTRQPYFRWLGPTAIAPPKDGTFRLLSVNLVSDEPDNGGKYVVV
ERQDKQSLYSKSSRSPTIPVREQIDTKAQSYAENLPPPPKSLYKLFNDKMANFLPYLP
HDIFEERLADGVISECLLFAMASLAERMAANSRKEYDSIGEENDKSQDNTEGTDEISL
AEKYAEAAKRLIIPHLSCPSVEIVFALLLIAYCEFGDDRDSGLWSWSGMAIRMCYDLG
LQKANTNEPDSWFSRNVFWSVVCLDRLISCGTGRATTIPDSVIEYELQPRWVKDSDGK
SRSDPFPYLCKLLLIVGKVSNYLNSCAESDAQKLMYNSKIGHPLSRSQIDMIEKFSAF
QQEVSDFYTNLPPDLLFDVQNFQCFAKIKCSPVFLLLHIWNQALVLAVHQPSLVYPKC
QLDITGLDQYPHAELTGSGAISIADMVTFADLIEPDSFLANPFLSQPIFMAACASLTL
RFSLRAPYQSQPIYKLERTYNTCRQTLNRMETIWRGISWHSRTLDSLAASEPDVDLTI
NSKVYVTTKDLGVVRRASVDEATRDWLAGEIRGRQVDDIYGMFIAGTIDGGVGNNQYE
TSPSSTGTEPRSDGFHDIGNRLNENGMMLFDSPQPTSNTTLIEEIFKIF"
gene complement(<964835..>964906)
/locus_tag="AWJ20_2313"
/db_xref="GeneID:30034211"
tRNA complement(<964835..>964906)
/locus_tag="AWJ20_2313"
/product="tRNA-His"
/db_xref="GeneID:30034211"
gene complement(<965405..>965476)
/locus_tag="AWJ20_2314"
/db_xref="GeneID:30034212"
tRNA complement(<965405..>965476)
/locus_tag="AWJ20_2314"
/product="tRNA-His"
/db_xref="GeneID:30034212"
gene complement(<965639..>966403)
/gene="SDS22"
/locus_tag="AWJ20_2315"
/db_xref="GeneID:30034213"
mRNA complement(<965639..>966403)
/gene="SDS22"
/locus_tag="AWJ20_2315"
/product="Sds22p"
/transcript_id="XM_018879252.1"
/db_xref="GeneID:30034213"
CDS complement(965639..966403)
/gene="SDS22"
/locus_tag="AWJ20_2315"
/inference="similar to AA sequence:KEGG_Orthology:K17550"
/note="Regulatory subunit of the type 1 protein
phosphatase (PP1) Glc7p; whether it functions as a
positive or negative regulator of Glc7p is controversial;
involved in the regulation of Glc7p nuclear localization
and function; GO_component: GO:0005737 - cytoplasm
[Evidence IDA] [PMID 11914276]; GO_component: GO:0005737 -
cytoplasm [Evidence IDA] [PMID 14562095]; GO_component:
GO:0005634 - nucleus [Evidence IEA,IEA]; GO_component:
GO:0005634 - nucleus [Evidence IDA] [PMID 11801737];
GO_component: GO:0005634 - nucleus [Evidence IDA] [PMID
11914276]; GO_component: GO:0005634 - nucleus [Evidence
IDA] [PMID 14562095]; GO_component: GO:0000164 - protein
phosphatase type 1 complex [Evidence IDA] [PMID 10775415];
GO_function: GO:0071862 - protein phosphatase type 1
activator activity [Evidence IGI] [PMID 11801737];
GO_function: GO:0071862 - protein phosphatase type 1
activator activity [Evidence IGI] [PMID 18172024];
GO_function: GO:0004865 - protein serine/threonine
phosphatase inhibitor activity [Evidence IGI,IMP] [PMID
17142459]; GO_process: GO:0007049 - cell cycle [Evidence
IEA]; GO_process: GO:0051301 - cell division [Evidence
IEA]; GO_process: GO:0007059 - chromosome segregation
[Evidence IMP] [PMID 11801737]; GO_process: GO:0051457 -
maintenance of protein location in nucleus [Evidence IMP]
[PMID 11801737]; GO_process: GO:0007067 - mitotic nuclear
division [Evidence IEA]"
/codon_start=1
/product="Sds22p"
/protein_id="XP_018737185.1"
/db_xref="GeneID:30034213"
/translation="MDGLEDLPEDLEELDLYDNRLTHVGHLDHFTPKMTTLDLSFNNI
KHIKHLDHLTGLSSLYLCQNQISKIVGLDQLKNITNLELGANKIREIKNLDGLVNLEQ
LWLGKNKITKLENLGHLKKLKILSIQANRIVKLEGLEELESLEELYISDNGIEKIEGL
DNNTKLTTLDVSSNRISELTNLRGLVNLEEFWASRNKISSFKNVEDELGKLPNLHTVY
FEHNPLQLENKVTYRNKLRLALGPSLQQIDATYIRN"
gene complement(<969267..>969593)
/gene="ACP1"
/locus_tag="AWJ20_2316"
/db_xref="GeneID:30034214"
mRNA complement(<969267..>969593)
/gene="ACP1"
/locus_tag="AWJ20_2316"
/product="Acp1p"
/transcript_id="XM_018879253.1"
/db_xref="GeneID:30034214"
CDS complement(969267..969593)
/gene="ACP1"
/locus_tag="AWJ20_2316"
/inference="similar to AA sequence:KEGG_Orthology:K03955"
/note="Mitochondrial matrix acyl carrier protein; involved
in biosynthesis of octanoate, which is a precursor to
lipoic acid; activated by phosphopantetheinylation
catalyzed by Ppt2p; GO_component: GO:0005739 -
mitochondrion [Evidence IEA,IEA]; GO_component: GO:0005739
- mitochondrion [Evidence IDA] [PMID 14576278];
GO_component: GO:0005739 - mitochondrion [Evidence IDA]
[PMID 16823961]; GO_component: GO:0070469 - respiratory
chain [Evidence IEA]; GO_function: GO:0000036 - ACP
phosphopantetheine attachment site binding involved in
fatty acid biosynthetic process [Evidence ISS] [PMID
8394042]; GO_process: GO:0006633 - fatty acid biosynthetic
process [Evidence IEA,IEA,IEA]; GO_process: GO:0006631 -
fatty acid metabolic process [Evidence IEA]; GO_process:
GO:0006629 - lipid metabolic process [Evidence IEA];
GO_process: GO:0009107 - lipoate biosynthetic process
[Evidence IMP] [PMID 9187370]; GO_process: GO:0055114 -
oxidation-reduction process [Evidence IEA]"
/codon_start=1
/product="Acp1p"
/protein_id="XP_018737186.1"
/db_xref="GeneID:30034214"
/translation="MFRMNASLIAKRASAAVTCRAAGSAAVRRPAFVNMLAMSRSYSA
SASGLNQKDVEDRIIKVFRAFDKIEDASKITPTANFSTELGLDSLDTVEVIVAVEEEF
GIEIPE"
gene complement(<971377..>973071)
/locus_tag="AWJ20_2317"
/db_xref="GeneID:30034215"
mRNA complement(<971377..>973071)
/locus_tag="AWJ20_2317"
/product="Myosin-Vb"
/transcript_id="XM_018879254.1"
/db_xref="GeneID:30034215"
CDS complement(971377..973071)
/locus_tag="AWJ20_2317"
/codon_start=1
/product="Myosin-Vb"
/protein_id="XP_018737187.1"
/db_xref="GeneID:30034215"
/translation="MAHPIAPAGTAKPEPADFGGPRLVPRLSKPERPTGLVTSKKWVV
PPRPKPGRKPGSKEGAKKRAVAAGAGGENGSGDSFTALREQLEKSRKENDELRNLVDE
FRSTSRSGSPVSNVPTVSVSKSGIVEGTVEPRQEEVWDGVRLLNQSNDAPLEASSDCG
FCDREGGCVCEDLGLKAGSSRTHVHEQDVITEKLESQRHNHSHNRTHNYGPHHDHSGN
HDHNEHSAQAHVDVHNNVHTHSQINGHASRNGLLPGETDLNTLENGSGSRSLQSAVRH
THHPSALAQFDHFSPVKAVPLKRSPRRVGNNGTTNPIVKKFKRLGEPAQITLTTGTEI
PANSKLVSETSEGTSPFEVGLSNTGFNAVEEADAKTSAHANPCGFCSDESPCLCADER
EGRTTTPTTTSTTSSSNGAISSESRGSFSMSPKGTRHFNNSTTVSSPSSVSLPPVSSV
TSRPPPYQQNVPSSSNPSLITPTGISTNPGSCTQCLSDPMSTLFCTSLSNVSAGSEVP
PEKLGMTVTCSQAYQVLSRHQKFPTYDLGAIVSTLISHDGKVGVQSITQALRKLDT"
gene complement(<973386..>975245)
/locus_tag="AWJ20_2318"
/db_xref="GeneID:30034216"
mRNA complement(<973386..>975245)
/locus_tag="AWJ20_2318"
/product="hypothetical protein"
/transcript_id="XM_018879255.1"
/db_xref="GeneID:30034216"
CDS complement(973386..975245)
/locus_tag="AWJ20_2318"
/codon_start=1
/product="hypothetical protein"
/protein_id="XP_018737188.1"
/db_xref="GeneID:30034216"
/translation="MNDTQQDIWRGSIMIVTNDATSDYSTPPFLNVTIEPLVDPSASA
NTGVEGSALNGGSASHGNTTTVESNKIYGEYGVSFWRFDLNLNLIDSEQRVTYSINAG
SDSLGSSSPSPTETFYIPSRNQDMNIMFYSCNGFSLGVAPEKFKGQLWNDVLEQHENK
YHFHVMLGGGDQLYCDKVKIATESVKKWSEEKNPFKKRTAPMQPEEKAEIDQFYLWHY
ISWFGLGYFYGPHGRTLQPGWPKALAKIPMINIFDDHDIIDGFGSYTDGIMKSPHFSG
IGRAAFKYYLLFQHCVAPEEPTSKEPSWVMSPRPGPYMGEHARSVYARLGQSIAFYGL
DCRTERSHHQVCEDATYDAMFSRINHEVKQSNGQIKHLVIMLGVPIAYPRMVWLERIL
TSDLLMPLKMLTRKGYFSGLSNEFDDGIEILDDLDDHWCARHHKKERNEFVSRMQAFS
KSTGVRITILGGDVHLAGVGRFYSRKPVPPEKDYRHMLNIISSAIVNTPPPDKLADFL
NKRNKIHHFGSDTDEDMVKIFPVDVDNSHRNNDRLLPRRNWCSIIATHGQGQARRAGQ
INSTGEPPVSNSRETLYPDLPGTLSVILHMEKNDQDPASVTIPYEVLVPPLEL"
gene complement(<976463..>978091)
/gene="SST2"
/locus_tag="AWJ20_2319"
/db_xref="GeneID:30034217"
mRNA complement(<976463..>978091)
/gene="SST2"
/locus_tag="AWJ20_2319"
/product="GTPase-activating protein SST2"
/transcript_id="XM_018879256.1"
/db_xref="GeneID:30034217"
CDS complement(976463..978091)
/gene="SST2"
/locus_tag="AWJ20_2319"
/codon_start=1
/product="GTPase-activating protein SST2"
/protein_id="XP_018737189.1"
/db_xref="GeneID:30034217"
/translation="MVLSLRLSSKPQKEPSLFFARSFPFSFSVSDALKAMKNMSISVD
NGSTVTIIKCGIPDNGEYFLDKFLTAKLLHCPGSRTRETIKASVLLQPTAKGFHFVER
YCRRNGISNDNVSEMLLSEYNSMQCICLDRHHLTGEIMRSEGIVKLLFQRFMGPSPNT
YDASNPPDEIAINNLSRIYGQKVELSLHSPISLIFETGNMDQRVSPYAHRYFTHPDSD
SLMQYYVSSKGVRLHQTREFYDKRKSRVSVDFCVSGKAAVQWLSDCTDTIKPSESFAL
ASLFLKYKLLEPITLEPSISGSSDNIVVSRQAYYQLSDRGRRMVFWDRDHFFRHKSQV
EQTEEYEEDEFYTPGSSFVMDEDDESDEVSNSKHSSPGRNNNTNTSAITLQMILSDPA
MCHLFREYLEENLCQENLLFYKDMEKISDMYKHLKLLKKLPNDKEPIESYSTTILSAI
YGVLGRYFSPRAPYGLNIDAQIKRMIIEEMTTNQSLTNDNKNNNNKHETSALVDRMEP
LLEKARELAVKMMSQDSLQKFLRSSYYLSAVRSC"
gene complement(<978777..>980540)
/gene="MKK2"
/locus_tag="AWJ20_2320"
/db_xref="GeneID:30034219"
mRNA complement(<978777..>980540)
/gene="MKK2"
/locus_tag="AWJ20_2320"
/product="putative mitogen-activated protein kinase kinase
MKK2"
/transcript_id="XM_018879257.1"
/db_xref="GeneID:30034219"
CDS complement(978777..980540)
/gene="MKK2"
/locus_tag="AWJ20_2320"
/inference="similar to AA sequence:KEGG_Orthology:K08294"
/codon_start=1
/product="putative mitogen-activated protein kinase kinase
MKK2"
/protein_id="XP_018737190.1"
/db_xref="GeneID:30034219"
/translation="MSAPPLIRPPPKPASRMKLPGLQIPNSQSRSMSPVISPPKDEDI
SMSDSSSTTLLSSGLPTATPQYHNPTLATTTAKSIYTASASTSTPSLPSIITSDHQSI
ASLPMPVPRKSAHPFSHSNNSSSTGLHLSLNTNSETYGSYGSYGVSSTGDVTGTSNIG
GPYGSYGSYGSASTSLIVPPENYPSNRSPLSAASGLFSAEVLAAVRVGDDSSSLNDSD
VDHSMTVDSKSSSSGTSRLTATDSLGANGGLSTSVSSNSNSGYTTPNGSYLLSQSTSN
KSLSSEYDVEDLDEEGWAMVARQGDIVDMGRLGEGSGGSVSRCRLKNGKTIFALKEIT
TNPNPELQKQILRELQFNRSCNSPHIVKYYGTFLSNEFSSIFIAMEYCGGGSLEAIYK
RVKARNGRIGEKVLGKIAEGVLSGLSYLHERKIIHRDIKPQNILLDAKGQVKLCDFGV
SGEVVDSLATTFTGTSYYMAPERIQGQPYTVTSDVWSLGLTLMEVAQHRFPFLTEGHD
PLMPIELLTFIVNMPAPELQDEPQEGIKWSNSFRHFLSCCLEKDPKKRASPRQMLSHP
WMVGQRSKTVRMDKFVKECWN"
gene <984023..>984829
/gene="NAS6"
/locus_tag="AWJ20_2321"
/db_xref="GeneID:30034220"
mRNA <984023..>984829
/gene="NAS6"
/locus_tag="AWJ20_2321"
/product="Nas6p"
/transcript_id="XM_018879258.1"
/db_xref="GeneID:30034220"
CDS 984023..984829
/gene="NAS6"
/locus_tag="AWJ20_2321"
/inference="similar to AA sequence:KEGG_Orthology:K06694"
/note="Assembly chaperone for the 19S proteasome
regulatory particle base; proteasome-interacting protein
involved in the assembly of the base subcomplex of the 19S
proteasomal regulatory particle (RP); ortholog of human
oncoprotein gankyrin, which interacts with the Rb tumor
suppressor and CDK4/6; GO_component: GO:0005829 - cytosol
[Evidence IDA] [PMID 19446323]; GO_component: GO:0005634 -
nucleus [Evidence IDA] [PMID 19446323]; GO_component:
GO:0005838 - proteasome regulatory particle [Evidence IDA]
[PMID 11029046]; GO_function: GO:0003674 -
molecular_function [Evidence ND]; GO_process: GO:0070682 -
proteasome regulatory particle assembly [Evidence IGI]
[PMID 19412159]; GO_process: GO:0070682 - proteasome
regulatory particle assembly [Evidence IGI,IMP] [PMID
19446322]; GO_process: GO:0070682 - proteasome regulatory
particle assembly [Evidence IGI,IMP] [PMID 19446323];
GO_process: GO:0006508 - proteolysis [Evidence IPI,ISS]
[PMID 11029046]"
/codon_start=1
/product="Nas6p"
/protein_id="XP_018737191.1"
/db_xref="GeneID:30034220"
/translation="MTESHGNSEASQAGKPLNEDSTPLALSENAESWPLHDAARDGKA
LLVKSILAENGKLVNSKDQDGRTPLFWAISTGGAEVTSTILKFVRDTRPRGFDLEEGD
EAGWTVVHISASTGQLDVIKQLVDIAKTVFSDDLDLDVQTKRGQTPLHFAVSKSHIDV
VKYLLEKGASPRTKDALNQNPLHRAAAVGSLPIVKLLIAAKTPLNATDRSGWTPLHHA
LAEGHGDVAIELISAGADQDRADAEGHTPMDVVVDDKVKKYVQVALSKQH"
gene complement(<985327..>985887)
/gene="RPL18A"
/locus_tag="AWJ20_2322"
/db_xref="GeneID:30034221"
mRNA complement(<985327..>985887)
/gene="RPL18A"
/locus_tag="AWJ20_2322"
/product="ribosomal 60S subunit protein L18A"
/transcript_id="XM_018879259.1"
/db_xref="GeneID:30034221"
CDS complement(985327..985887)
/gene="RPL18A"
/locus_tag="AWJ20_2322"
/inference="similar to AA sequence:KEGG_Orthology:K02883"
/note="Ribosomal 60S subunit protein L18A; intron of
RPL18A pre-mRNA forms stem-loop structures that are a
target for Rnt1p cleavage leading to degradation;
homologous to mammalian ribosomal protein L18, no
bacterial homolog; RPL18A has a paralog, RPL18B, that
arose from the whole genome duplication; GO_component:
GO:0005737 - cytoplasm [Evidence IEA,IEA]; GO_component:
GO:0022625 - cytosolic large ribosomal subunit [Evidence
IDA] [PMID 11983894]; GO_component: GO:0005622 -
intracellular [Evidence IEA]; GO_component: GO:0030529 -
ribonucleoprotein complex [Evidence IEA]; GO_component:
GO:0005840 - ribosome [Evidence IEA,IEA]; GO_function:
GO:0003735 - structural constituent of ribosome [Evidence
IEA]; GO_function: GO:0003735 - structural constituent of
ribosome [Evidence IC] [PMID 11983894]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence IC] [PMID
11983894]; GO_process: GO:0006412 - translation [Evidence
IEA]"
/codon_start=1
/product="ribosomal 60S subunit protein L18A"
/protein_id="XP_018737192.1"
/db_xref="GeneID:30034221"
/translation="MGKDQFSKEHIRNSHRTAPKSDNVYLSLLVKLYRFLARRTDSNF
NKVILKSLFLSKINRPPVSVSRITRALKQKGAENKTIVVVGTVTDDNRLFEVPKTTVA
ALRFTAGARARIVKAGGEAITLDQLARRAPTGQNTLILRGPRASREAVRHFGMGPHKG
KAPRIQSKGRKFERARGRRRSRGFKV"
gene complement(<988923..>990764)
/gene="SMI1"
/locus_tag="AWJ20_2323"
/db_xref="GeneID:30034222"
mRNA complement(<988923..>990764)
/gene="SMI1"
/locus_tag="AWJ20_2323"
/product="Smi1p"
/transcript_id="XM_018879260.1"
/db_xref="GeneID:30034222"
CDS complement(988923..990764)
/gene="SMI1"
/locus_tag="AWJ20_2323"
/note="Protein involved in the regulation of cell wall
synthesis; proposed to be involved in coordinating cell
cycle progression with cell wall integrity; GO_component:
GO:0005935 - cellular bud neck [Evidence IDA] [PMID
10206705]; GO_component: GO:0000131 - incipient cellular
bud site [Evidence IDA] [PMID 10206705]; GO_component:
GO:0043332 - mating projection tip [Evidence IDA] [PMID
19053807]; GO_component: GO:0005634 - nucleus [Evidence
IEA,IEA]; GO_function: GO:0003677 - DNA binding [Evidence
IEA]; GO_function: GO:0003674 - molecular_function
[Evidence ND]; GO_process: GO:0042546 - cell wall
biogenesis [Evidence IEA]; GO_process: GO:0032995 -
regulation of fungal-type cell wall biogenesis [Evidence
IGI,IMP] [PMID 10206705]; GO_process: GO:0007346 -
regulation of mitotic cell cycle [Evidence IGI] [PMID
11410349]; GO_process: GO:0007346 - regulation of mitotic
cell cycle [Evidence IMP] [PMID 12823808]; GO_process:
GO:0006355 - regulation of transcription, DNA-templated
[Evidence IEA]; GO_process: GO:0006351 - transcription,
DNA-templated [Evidence IEA]"
/codon_start=1
/product="Smi1p"
/protein_id="XP_018737193.1"
/db_xref="GeneID:30034222"
/translation="MDKIFSSVQSFLHSVTTNDRYASYESQYRSSPNGRGPGASTKYR
RTPLGVTGNSVHDEIDGGSGSGTPPNGQNGPYGYRNSSSTSLDGPNSRNGSNTNLASS
STTSLNRGPYTPGMRSAQMGNGGNIPLQDYSDGIPAPPAAALSWKRIDRWVDANYPEL
YDQLSYPATSADLNELESDLDCTLPLEVRDSYMVHDGQERGGRPTGLFFNICILDLEG
IVEEWTHWKNTAIRLNNVVRSQRQQKQALSSSSAAASSSSSSQQQLRSVSASSSSSSS
ALVPPHMKQQAQQTPQQIQQQQQHYKNTLNWIQRQESVPEGAIQPVYAHPAWIPLAKD
FEGNNIAVDLAPGKKGRWGQVILFGRDFDRKYVIAPSWGAFLASFADDLEAGDHSIDE
DLEEGSLMFRAPNGRLIPYFDVLKSRVERSVRLARKQQGIQSPVIGSNGAGSMPGTRI
SRPSTPGHHGASSNSTITNGSTGTGANKANGSAAGSAPGRNLSSPKGQNLISPFQSTT
NLATPTSEPSESQSESKENTEELSEPAAETKTEDKPETKTDSEPATKSNDKPEPESKK
ESEADENPETTATESTESKKDTSASASASASDDEVDMLEDELTEVQI"
gene <994160..>994438
/gene="RPS15"
/locus_tag="AWJ20_2324"
/db_xref="GeneID:30034223"
mRNA <994160..>994438
/gene="RPS15"
/locus_tag="AWJ20_2324"
/product="ribosomal 40S subunit protein S15"
/transcript_id="XM_018879261.1"
/db_xref="GeneID:30034223"
CDS 994160..994438
/gene="RPS15"
/locus_tag="AWJ20_2324"
/inference="similar to AA sequence:KEGG_Orthology:K02958"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S15 and
bacterial S19; GO_component: GO:0005737 - cytoplasm
[Evidence IEA,IEA]; GO_component: GO:0022627 - cytosolic
small ribosomal subunit [Evidence NAS] [PMID 9559554];
GO_component: GO:0030529 - ribonucleoprotein complex
[Evidence IEA]; GO_component: GO:0005840 - ribosome
[Evidence IEA,IEA]; GO_component: GO:0015935 - small
ribosomal subunit [Evidence IEA]; GO_function: GO:0003723
- RNA binding [Evidence IEA]; GO_function: GO:0003735 -
structural constituent of ribosome [Evidence IEA];
GO_function: GO:0003735 - structural constituent of
ribosome [Evidence NAS] [PMID 9559554]; GO_process:
GO:0002181 - cytoplasmic translation [Evidence NAS] [PMID
9559554]; GO_process: GO:0006407 - rRNA export from
nucleus [Evidence IMP] [PMID 15167894]; GO_process:
GO:0006407 - rRNA export from nucleus [Evidence IMP] [PMID
16246728]; GO_process: GO:0042254 - ribosome biogenesis
[Evidence IEA]; GO_process: GO:0006412 - translation
[Evidence IEA]"
/codon_start=1
/product="ribosomal 40S subunit protein S15"
/protein_id="XP_018737194.1"
/db_xref="GeneID:30034223"
/translation="MRFVKKLRAAKLAAGPNEKPAVVKTHLRNMLVLPEMIGSVIGVY
NGKVFNQVEIKPEMVGHYLGEFSITYRPVAHKRPGAGSTKDSRFIPLK"
gene complement(<994715..>996325)
/gene="MTC6"
/locus_tag="AWJ20_2325"
/db_xref="GeneID:30034224"
mRNA complement(<994715..>996325)
/gene="MTC6"
/locus_tag="AWJ20_2325"
/product="Mtc6p"
/transcript_id="XM_018879262.1"
/db_xref="GeneID:30034224"
CDS complement(994715..996325)
/gene="MTC6"
/locus_tag="AWJ20_2325"
/note="hypothetical protein; mtc6 is synthetically sick
with cdc13-1; GO_component: GO:0005575 -
cellular_component [Evidence ND]; GO_component: GO:0016021
- integral component of membrane [Evidence IEA];
GO_component: GO:0016020 - membrane [Evidence IEA,IEA];
GO_function: GO:0003674 - molecular_function [Evidence
ND]; GO_process: GO:0008150 - biological_process [Evidence
ND]"
/codon_start=1
/product="Mtc6p"
/protein_id="XP_018737195.1"
/db_xref="GeneID:30034224"
/translation="MSSFSLDAFWSNTNSQWQLCPQPFPQGVSTSGQIYALDDGIKCQ
DNVTLDSVYSVIATYIQQTETSAIATNLLTLRLNLNQLNQSIDVENNVRGDYVLSTNI
SSIFGPRVYTPLDLENDRKNGVVSNIDEGNNFPPLHYFLLSINKRILVLADDSQLIQS
ISTGMSSTSSSSSTATSISTTGSGKHTGTSSSTTTTTTTTSTPTPSFSQNINGDYQVL
FVNSNITFQEEQSLTLTNDLVNTCANNGDQPSQQDLINHINETSMIPFRAVFDTPDFP
FTDVTLGIISQCGYTPMLNSSSSLTDPMSNSAQLTPQILVDVVNNSFWSWAPGEPNTN
KTNASKPKSITDPVALQCAVITPEGWKVANCYEPFPVMCRGNDSNFDWIATGNKSTYF
DAPQSCPKGTNFSVPRTSLENLAARSVLQQEVPAPKPSSDSSDDSNSSPGGSINVPAA
WIDFNTIAVDECWVTGGPFSACPYLQIPTTNRNGVALLSIAAVVAFVLLMAMILLNFR
RIPLRRNQARWRRLINKFAEQEYEGVPL"
gene 