LOCUS NC_002640 10649 bp ss-RNA linear VRL 11-JUL-2019
DEFINITION Dengue virus 4, complete genome.
ACCESSION NC_002640
VERSION NC_002640.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE dengue virus type 4
ORGANISM dengue virus type 4
Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Flasuviricetes;
Amarillovirales; Flaviviridae; Orthoflavivirus; Orthoflavivirus
denguei.
REFERENCE 1 (bases 1 to 10649)
AUTHORS Durbin,A.P., Karron,R.A., Sun,W., Vaughn,D.W., Reynolds,M.J.,
Perreault,J.R., Men,R.H., Lai,C.J., Elkins,W.R., Chanock,R.M.,
Murphy,B.R. and Whitehead,S.S.
TITLE A live attenuated dengue virus type 4 vaccine candidate with a 30
nucleotide deletion in the 3' untranslated region is highly
attenuated and immunogenic in humans
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10649)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2001) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 10649)
AUTHORS Whitehead,S.S.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2000) LID, NIAID, 7 Center Drive, Bethesda, MD
20892, USA
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AF326825.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..10649
/organism="dengue virus type 4"
/mol_type="genomic RNA"
/db_xref="taxon:11070"
/clone="rDEN4"
5'UTR 1..101
stem_loop 2..69
/note="stem-loop A (SLA)"
regulatory 70..80
/regulatory_class="other"
/note="oligo U track spacer"
regulatory 81..96
/regulatory_class="promoter"
/note="5' upstream AUG region (UAR)"
stem_loop 81..95
/note="stem-loop B (SLB)"
gene 102..10265
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/db_xref="GeneID:5075729"
CDS 102..10265
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/codon_start=1
/product="polyprotein"
/protein_id="NP_073286.1"
/db_xref="GeneID:5075729"
/translation="MNQRKKVVRPPFNMLKRERNRVSTPQGLVKRFSTGLFSGKGPLR
MVLAFITFLRVLSIPPTAGILKRWGQLKKNKAIKILIGFRKEIGRMLNILNGRKRSTI
TLLCLIPTVMAFSLSTRDGEPLMIVAKHERGRPLLFKTTEGINKCTLIAMDLGEMCED
TVTYKCPLLVNTEPEDIDCWCNLTSTWVMYGTCTQSGERRREKRSVALTPHSGMGLET
RAETWMSSEGAWKHAQRVESWILRNPGFALLAGFMAYMIGQTGIQRTVFFVLMMLVAP
SYGMRCVGVGNRDFVEGVSGGAWVDLVLEHGGCVTTMAQGKPTLDFELTKTTAKEVAL
LRTYCIEASISNITTATRCPTQGEPYLKEEQDQQYICRRDVVDRGWGNGCGLFGKGGV
VTCAKFSCSGKITGNLVQIENLEYTVVVTVHNGDTHAVGNDTSNHGVTAMITPRSPSV
EVKLPDYGELTLDCEPRSGIDFNEMILMKMKKKTWLVHKQWFLDLPLPWTAGADTSEV
HWNYKERMVTFKVPHAKRQDVTVLGSQEGAMHSALAGATEVDSGDGNHMFAGHLKCKV
RMEKLRIKGMSYTMCSGKFSIDKEMAETQHGTTVVKVKYEGAGAPCKVPIEIRDVNKE
KVVGRIISSTPLAENTNSVTNIELEPPFGDSYIVIGVGNSALTLHWFRKGSSIGKMFE
STYRGAKRMAILGETAWDFGSVGGLFTSLGKAVHQVFGSVYTTMFGGVSWMIRILIGF
LVLWIGTNSRNTSMAMTCIAVGGITLFLGFTVQADMGCVASWSGKELKCGSGIFVVDN
VHTWTEQYKFQPESPARLASAILNAHKDGVCGIRSTTRLENVMWKQITNELNYVLWEG
GHDLTVVAGDVKGVLTKGKRALTPPVSDLKYSWKTWGKAKIFTPEARNSTFLIDGPDT
SECPNERRAWNSLEVEDYGFGMFTTNIWMKFREGSSEVCDHRLMSAAIKDQKAVHADM
GYWIESSKNQTWQIEKASLIEVKTCLWPKTHTLWSNGVLESQMLIPKSYAGPFSQHNY
RQGYATQTVGPWHLGKLEIDFGECPGTTVTIQEDCDHRGPSLRTTTASGKLVTQWCCR
SCTMPPLRFLGEDGCWYGMEIRPLSEKEENMVKSQVTAGQGTSETFSMGLLCLTLFVE
ECLRRRVTRKHMILVVVITLCAIILGGLTWMDLLRALIMLGDTMSGRIGGQIHLAIMA
VFKMSPGYVLGVFLRKLTSRETALMVIGMAMTTVLSIPHDLMELIDGISLGLILLKIV
TQFDNTQVGTLALSLTFIRSTMPLVMAWRTIMAVLFVVTLIPLCRTSCLQKQSHWVEI
TALILGAQALPVYLMTLMKGASRRSWPLNEGIMAVGLVSLLGSALLKNDVPLAGPMVA
GGLLLAAYVMSGSSADLSLEKAANVQWDEMADITGSSPIVEVKQDEDGSFSIRDVEET
NMITLLVKLALITVSGLYPLAIPVTMTLWYMWQVKTQRSGALWDVPSPAATKKAALSE
GVYRIMQRGLFGKTQVGVGIHMEGVFHTMWHVTRGSVICHETGRLEPSWADVRNDMIS
YGGGWRLGDKWDKEEDVQVLAIEPGKNPKHVQTKPGLFKTLTGEIGAVTLDFKPGTSG
SPIINRKGKVIGLYGNGVVTKSGDYVSAITQAERIGEPDYEVDEDIFRKKRLTIMDLH
PGAGKTKRILPSIVREALKRRLRTLILAPTRVVAAEMEEALRGLPIRYQTPAVKSEHT
GREIVDLMCHATFTTRLLSSTRVPNYNLIVMDEAHFTDPSSVAARGYISTRVEMGEAA
AIFMTATPPGATDPFPQSNSPIEDIEREIPERSWNTGFDWITDYQGKTVWFVPSIKAG
NDIANCLRKSGKKVIQLSRKTFDTEYPKTKLTDWDFVVTTDISEMGANFRAGRVIDPR
RCLKPVILPDGPERVILAGPIPVTPASAAQRRGRIGRNPAQEDDQYVFSGDPLKNDED
HAHWTEAKMLLDNIYTPEGIIPTLFGPEREKTQAIDGEFRLRGEQRKTFVELMRRGDL
PVWLSYKVASAGISYEDREWCFTGERNNQILEENMEVEIWTREGEKKKLRPRWLDARV
YADPMALKDFKEFASGRKSITLDILTEIASLPTYLSSRAKLALDNIVMLHTTERGGRA
YQHALNELPESLETLMLVALLGAMTAGIFLFFMQGKGIGKLSMGLITIAVASGLLWVA
EIQPQWIAASIILEFFLMVLLIPEPEKQRTPQDNQLIYVILTILTIIGLIAANEMGLI
EKTKTDFGFYQVKTETTILDVDLRPASAWTLYAVATTILTPMLRHTIENTSANLSLAA
IANQAAVLMGLGKGWPLHRMDLGVPLLAMGCYSQVNPTTLTASLVMLLVHYAIIGPGL
QAKATREAQKRTAAGIMKNPTVDGITVIDLEPISYDPKFEKQLGQVMLLVLCAGQLLL
MRTTWAFCEVLTLATGPILTLWEGNPGRFWNTTIAVSTANIFRGSYLAGAGLAFSLIK
NAQTPRRGTGTTGETLGEKWKRQLNSLDRKEFEEYKRSGILEVDRTEAKSALKDGSKI
KHAVSRGSSKIRWIVERGMVKPKGKVVDLGCGRGGWSYYMATLKNVTEVKGYTKGGPG
HEEPIPMATYGWNLVKLHSGVDVFYKPTEQVDTLLCDIGESSSNPTIEEGRTLRVLKM
VEPWLSSKPEFCIKVLNPYMPTVIEELEKLQRKHGGNLVRCPLSRNSTHEMYWVSGAS
GNIVSSVNTTSKMLLNRFTTRHRKPTYEKDVDLGAGTRSVSTETEKPDMTIIGRRLQR
LQEEHKETWHYDQENPYRTWAYHGSYEAPSTGSASSMVNGVVKLLTKPWDVIPMVTQL
AMTDTTPFGQQRVFKEKVDTRTPQPKPGTRMVMTTTANWLWALLGKKKNPRLCTREEF
ISKVRSNAAIGAVFQEEQGWTSASEAVNDSRFWELVDKERALHQEGKCESCVYNMMGK
REKKLGEFGRAKGSRAIWYMWLGARFLEFEALGFLNEDHWFGRENSWSGVEGEGLHRL
GYILEEIDKKDGDLMYADDTAGWDTRITEDDLQNEELITEQMAPHHKILAKAIFKLTY
QNKVVKVLRPTPRGAVMDIISRKDQRGSGQVGTYGLNTFTNMEVQLIRQMEAEGVITQ
DDMQNPKGLKERVEKWLKECGVDRLKRMAISGDDCVVKPLDERFGTSLLFLNDMGKVR
KDIPQWEPSKGWKNWQEVPFCSHHFHKIFMKDGRSLVVPCRNQDELIGRARISQGAGW
SLRETACLGKAYAQMWSLMYFHRRDLRLASMAICSAVPTEWFPTSRTTWSIHAHHQWM
TTEDMLKVWNRVWIEDNPNMTDKTPVHSWEDIPYLGKREDLWCGSLIGLSSRATWAKN
IHTAITQVRNLIGKEEYVDYMPVMKRYSAPSESEGVL"
mat_peptide 102..440
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="anchored capsid protein ancC"
/protein_id="NP_740314.1"
mat_peptide 102..398
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="capsid protein C"
/note="virion capsid protein"
/protein_id="NP_740313.1"
misc_feature 111..440
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus capsid protein C; Region: Flavi_capsid;
pfam01003"
/db_xref="CDD:366413"
mat_peptide 441..938
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="membrane glycoprotein precursor prM"
/protein_id="NP_740315.1"
mat_peptide 441..713
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="protein pr"
/note="peptide pr"
/protein_id="YP_009164957.1"
misc_feature 456..692
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus polyprotein propeptide; Region:
Flavi_propep; pfam01570"
/db_xref="CDD:366710"
mat_peptide 714..938
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="membrane glycoprotein M"
/protein_id="NP_740316.1"
misc_feature 714..938
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus envelope glycoprotein M; Region:
Flavi_M; cd17038"
/db_xref="CDD:341208"
misc_feature order(714..716,720..749,753..761,765..770,795..797,
885..887,918..920)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="glycoprotein E binding interface [polypeptide
binding]; other site"
/db_xref="CDD:341208"
mat_peptide 939..2423
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="envelope protein E"
/protein_id="NP_740317.1"
misc_feature 942..1826
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus glycoprotein, central and dimerization
domains; Region: Flavi_glycoprot; pfam00869"
/db_xref="CDD:395698"
misc_feature 1851..2120
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Immunoglobulin-like domain III (C-terminal domain)
of Flavivirus envelope glycoprotein E; Region: Flavi_E_C;
cd12149"
/db_xref="CDD:213392"
misc_feature order(1866..1871,1875..1880,1899..1904,2034..2036)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="homodimer interface [polypeptide binding]; other
site"
/db_xref="CDD:213392"
misc_feature order(1887..1889,1938..1940,1992..2003,2007..2009)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="low pH domain interface [polypeptide binding];
other site"
/db_xref="CDD:213392"
misc_feature order(1890..1892,1938..1940,1986..1994,2046..2048)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="low pH trimer interface [polypeptide binding];
other site"
/db_xref="CDD:213392"
misc_feature 2133..2423
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="flavivirus envelope glycoprotein E, stem/anchor
domain; Region: flavi_E_stem; TIGR04240"
/db_xref="CDD:213897"
mat_peptide 2424..3479
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="nonstructural protein NS1"
/protein_id="NP_740318.1"
misc_feature 2430..3488
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus non-structural Protein NS1; Region:
Flavi_NS1; pfam00948"
/db_xref="CDD:279316"
mat_peptide 3480..4133
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="nonstructural protein NS2A"
/protein_id="NP_740319.1"
misc_feature 3981..4256
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="MerC mercury resistance protein; Region: MerC;
pfam03203"
/db_xref="CDD:427195"
mat_peptide 4134..4523
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="nonstructural protein NS2B"
/protein_id="NP_740320.1"
mat_peptide 4524..6377
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="nonstructural protein NS3"
/protein_id="NP_740321.1"
misc_feature 4575..5024
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Peptidase S7, Flavivirus NS3 serine protease;
Region: Peptidase_S7; pfam00949"
/db_xref="CDD:395758"
misc_feature 5076..5513
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus DEAD domain; Region: Flavi_DEAD;
pfam07652"
/db_xref="CDD:400138"
misc_feature 5517..5951
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="C-terminal helicase domain of viral helicase;
Region: SF2_C_viral; cd18806"
/db_xref="CDD:350193"
misc_feature order(5538..5540,5553..5555,5562..5567,5829..5837)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="homodimer interface [polypeptide binding]; other
site"
/db_xref="CDD:350193"
misc_feature order(5901..5903,5910..5912)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="putative ATP binding site [chemical binding]; other
site"
/db_xref="CDD:350193"
mat_peptide 6378..6758
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="nonstructural protein NS4A"
/protein_id="NP_740322.1"
misc_feature 6390..6821
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus non-structural protein NS4A; Region:
Flavi_NS4A; pfam01350"
/db_xref="CDD:279666"
mat_peptide 6759..6827
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="protein 2K"
/protein_id="NP_740323.1"
mat_peptide 6828..7562
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="nonstructural protein NS4B"
/protein_id="NP_740324.1"
misc_feature 6828..7541
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Flavivirus non-structural protein NS4B; Region:
Flavi_NS4B; pfam01349"
/db_xref="CDD:279665"
mat_peptide 7563..10262
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/product="RNA-dependent RNA polymerase NS5"
/protein_id="NP_740325.1"
misc_feature 7596..8288
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="Cap-0 specific (nucleoside-2'-O-)-methyltransferase
of flaviviridae; Region: capping_2-OMTase_Flaviviridae;
cd20761"
/db_xref="CDD:467736"
misc_feature order(7602..7604,7611..7616,7620..7622,8010..8015,
8202..8204)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="nucleic acid substrate binding site [nucleotide
binding]; other site"
/db_xref="CDD:467736"
misc_feature order(7803..7811,7818..7823,7872..7877,7950..7955,
7998..8003)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="SAM binding site [chemical binding]; other site"
/db_xref="CDD:467736"
misc_feature 8520..10214
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="catalytic core domain of RNA-dependent RNA
polymerase (RdRp) in the genus Flavivirus, within the
family Flaviviridae of positive-sense single-stranded RNA
(+ssRNA) viruses; Region: Flavivirus_RdRp; cd23204"
/db_xref="CDD:438054"
misc_feature order(8766..8768,8778..8780,8799..8804,8994..8996,
9006..9008,9018..9020,9039..9041,9363..9383,9939..9941,
9948..9950,9954..9956)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="inhibitor binding site [chemical binding];
inhibition site"
/db_xref="CDD:438054"
misc_feature 8922..8987
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="conserved polymerase motif F; other site"
/db_xref="CDD:438054"
misc_feature order(8931..8933,8982..8984,8988..8990,9006..9008,
9018..9020,9030..9032,9042..9044,9081..9083,9087..9095,
9363..9374,9378..9383,9546..9557,9690..9695,9750..9752,
9774..9776,9822..9827,9834..9839,9843..9848)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="RNA binding site [nucleotide binding]; other site"
/db_xref="CDD:438054"
misc_feature order(8934..8936,8943..8945,8967..8969,8976..8978,
9177..9179,9360..9365,9390..9392,9552..9557,9693..9695,
9750..9752,9774..9776,9945..9953)
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="NTP binding site [chemical binding]; other site"
/db_xref="CDD:438054"
misc_feature 9147..9191
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438054"
misc_feature 9357..9428
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438054"
misc_feature 9525..9575
/gene="POLY"
/locus_tag="DV4_gp1"
/gene_synonym="polyprotein gene"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438054"
stem_loop 118..134
/note="capsid region hairpin (cHP)"
regulatory 136..146
/regulatory_class="other"
/note="5' conserved sequence (CS); also called cyclization
sequence"
3'UTR 10266..10649
ncRNA 10292..10649
/ncRNA_class="lncRNA"
/product="sfRNA2"
/note="subgenomic flavivirus RNA"
stem_loop 10296..10364
/note="flaviviral nuclease-resistant RNA 2 (fNR2); also
called stem-loop 2 or xrRNA2"
ncRNA 10372..10649
/ncRNA_class="lncRNA"
/product="sfRNA3"
/note="subgenomic flavivirus RNA"
stem_loop 10376..10462
/note="dumbbell 1 (DBI); also called xrRNA3"
ncRNA 10464..10649
/ncRNA_class="lncRNA"
/product="sfRNA4"
/note="subgenomic flavivirus RNA"
stem_loop 10467..10547
/note="dumbbell 2 (DBII); also called xrRNA4"
regulatory 10544..10554
/regulatory_class="other"
/note="3' conserved sequence (CS); also called cyclization
sequence"
stem_loop 10557..10570
/note="short hairpin (sHP)"
regulatory 10568..10584
/regulatory_class="promoter"
/note="3' upstream AUG region (UAR)"
stem_loop 10571..10649
/note="3' stem-loop (3'SL)"
ORIGIN
1 agttgttagt ctgtgtggac cgacaaggac agttccaaat cggaagcttg cttaacacag
61 ttctaacagt ttgtttgaat agagagcaga tctctggaaa aatgaaccaa cgaaaaaagg
121 tggttagacc acctttcaat atgctgaaac gcgagagaaa ccgcgtatca acccctcaag
181 ggttggtgaa gagattctca accggacttt tttctgggaa aggaccctta cggatggtgc
241 tagcattcat cacgtttttg cgagtccttt ccatcccacc aacagcaggg attctgaaga
301 gatggggaca gttgaagaaa aataaggcca tcaagatact gattggattc aggaaggaga
361 taggccgcat gctgaacatc ttgaacggga gaaaaaggtc aacgataaca ttgctgtgct
421 tgattcccac cgtaatggcg ttttccctca gcacaagaga tggcgaaccc ctcatgatag
481 tggcaaaaca tgaaaggggg agacctctct tgtttaagac aacagagggg atcaacaaat
541 gcactctcat tgccatggac ttgggtgaaa tgtgtgagga cactgtcacg tataaatgcc
601 ccctactggt caataccgaa cctgaagaca ttgattgctg gtgcaacctc acgtctacct
661 gggtcatgta tgggacatgc acccagagcg gagaacggag acgagagaag cgctcagtag
721 ctttaacacc acattcagga atgggattgg aaacaagagc tgagacatgg atgtcatcgg
781 aaggggcttg gaagcatgct cagagagtag agagctggat actcagaaac ccaggattcg
841 cgctcttggc aggatttatg gcttatatga ttgggcaaac aggaatccag cgaactgtct
901 tctttgtcct aatgatgctg gtcgccccat cctacggaat gcgatgcgta ggagtaggaa
961 acagagactt tgtggaagga gtctcaggtg gagcatgggt cgacctggtg ctagaacatg
1021 gaggatgcgt cacaaccatg gcccagggaa aaccaacctt ggattttgaa ctgactaaga
1081 caacagccaa ggaagtggct ctgttaagaa cctattgcat tgaagcctca atatcaaaca
1141 taactacggc aacaagatgt ccaacgcaag gagagcctta tctgaaagag gaacaggacc
1201 aacagtacat ttgccggaga gatgtggtag acagagggtg gggcaatggc tgtggcttgt
1261 ttggaaaagg aggagttgtg acatgtgcga agttttcatg ttcggggaag ataacaggca
1321 atttggtcca aattgagaac cttgaataca cagtggttgt aacagtccac aatggagaca
1381 cccatgcagt aggaaatgac acatccaatc atggagttac agccatgata actcccaggt
1441 caccatcggt ggaagtcaaa ttgccggact atggagaact aacactcgat tgtgaaccca
1501 ggtctggaat tgactttaat gagatgattc tgatgaaaat gaaaaagaaa acatggctcg
1561 tgcataagca atggtttttg gatctgcctc ttccatggac agcaggagca gacacatcag
1621 aggttcactg gaattacaaa gagagaatgg tgacatttaa ggttcctcat gccaagagac
1681 aggatgtgac agtgctggga tctcaggaag gagccatgca ttctgccctc gctggagcca
1741 cagaagtgga ctccggtgat ggaaatcaca tgtttgcagg acatcttaag tgcaaagtcc
1801 gtatggagaa attgagaatc aagggaatgt catacacgat gtgttcagga aagttttcaa
1861 ttgacaaaga gatggcagaa acacagcatg ggacaacagt ggtgaaagtc aagtatgaag
1921 gtgctggagc tccgtgtaaa gtccccatag agataagaga tgtaaacaag gaaaaagtgg
1981 ttgggcgtat catctcatcc acccctttgg ctgagaatac caacagtgta accaacatag
2041 aattagaacc cccctttggg gacagctaca tagtgatagg tgttggaaac agcgcattaa
2101 cactccattg gttcaggaaa gggagttcca ttggcaagat gtttgagtcc acatacagag
2161 gtgcaaaacg aatggccatt ctaggtgaaa cagcttggga ttttggttcc gttggtggac
2221 tgttcacatc attgggaaag gctgtgcacc aggtttttgg aagtgtgtat acaaccatgt
2281 ttggaggagt ctcatggatg attagaatcc taattgggtt cttagtgttg tggattggca
2341 cgaactcgag gaacacttca atggctatga cgtgcatagc tgttggagga atcactctgt
2401 ttctgggctt cacagttcaa gcagacatgg gttgtgtggc gtcatggagt gggaaagaat
2461 tgaagtgtgg aagcggaatt tttgtggttg acaacgtgca cacttggaca gaacagtaca
2521 aatttcaacc agagtcccca gcgagactag cgtctgcaat attaaatgcc cacaaagatg
2581 gggtctgtgg aattagatca accacgaggc tggaaaatgt catgtggaag caaataacca
2641 acgagctaaa ctatgttctc tgggaaggag gacatgacct cactgtagtg gctggggatg
2701 tgaagggggt gttgaccaaa ggcaagagag cactcacacc cccagtgagt gatctgaaat
2761 attcatggaa gacatgggga aaagcaaaaa tcttcacccc agaagcaaga aatagcacat
2821 ttttaataga cggaccagac acctctgaat gccccaatga acgaagagca tggaactctc
2881 ttgaggtgga agactatgga tttggcatgt tcacgaccaa catatggatg aaattccgag
2941 aaggaagttc agaagtgtgt gaccacaggt taatgtcagc tgcaattaaa gatcagaaag
3001 ctgtgcatgc tgacatgggt tattggatag agagctcaaa aaaccagacc tggcagatag
3061 agaaagcatc tcttattgaa gtgaaaacat gtctgtggcc caagacccac acactgtgga
3121 gcaatggagt gctggaaagc cagatgctca ttccaaaatc atatgcgggc cctttttcac
3181 agcacaatta ccgccagggc tatgccacgc aaaccgtggg cccatggcac ttaggcaaat
3241 tagagataga ctttggagaa tgccccggaa caacagtcac aattcaggag gattgtgacc
3301 atagaggccc atctttgagg accaccactg catctggaaa actagtcacg caatggtgct
3361 gccgctcctg cacgatgcct cccttaaggt tcttgggaga agatgggtgc tggtatggga
3421 tggagattag gcccttgagt gaaaaagaag agaacatggt caaatcacag gtgacggccg
3481 gacagggcac atcagaaact ttttctatgg gtctgttgtg cctgaccttg tttgtggaag
3541 aatgcttgag gagaagagtc actaggaaac acatgatatt agttgtggtg atcactcttt
3601 gtgctatcat cctgggaggc ctcacatgga tggacttact acgagccctc atcatgttgg
3661 gggacactat gtctggtaga ataggaggac agatccacct agccatcatg gcagtgttca
3721 agatgtcacc aggatacgtg ctgggtgtgt ttttaaggaa actcacttca agagagacag
3781 cactaatggt aataggaatg gccatgacaa cggtgctttc aattccacat gaccttatgg
3841 aactcattga tggaatatca ctgggactaa ttttgctaaa aatagtaaca cagtttgaca
3901 acacccaagt gggaacctta gctctttcct tgactttcat aagatcaaca atgccattgg
3961 tcatggcttg gaggaccatt atggctgtgt tgtttgtggt cacactcatt cctttgtgca
4021 ggacaagctg tcttcaaaaa cagtctcatt gggtagaaat aacagcactc atcctaggag
4081 cccaagctct gccagtgtac ctaatgactc ttatgaaagg agcctcaaga agatcttggc
4141 ctcttaacga gggcataatg gctgtgggtt tggttagtct cttaggaagc gctcttttaa
4201 agaatgatgt ccctttagct ggcccaatgg tggcaggagg cttacttctg gcggcttacg
4261 tgatgagtgg tagctcagca gatctgtcac tagagaaggc cgccaacgtg cagtgggatg
4321 aaatggcaga cataacaggc tcaagcccaa tcgtagaagt gaagcaggat gaagatggct
4381 ctttctccat acgggacgtc gaggaaacca atatgataac ccttttggtg aaactggcac
4441 tgataacagt gtcaggtctc taccccttgg caattccagt cacaatgacc ttatggtaca
4501 tgtggcaagt gaaaacacaa agatcaggag ccctgtggga cgtcccctca cccgctgcca
4561 ctaaaaaagc cgcactgtct gaaggagtgt acaggatcat gcaaagaggg ttattcggga
4621 aaactcaggt tggagtaggg atacacatgg aaggtgtatt tcacacaatg tggcatgtaa
4681 caagaggatc agtgatctgc cacgagactg ggagattgga gccatcttgg gctgacgtca
4741 ggaatgacat gatatcatac ggtgggggat ggaggcttgg agacaaatgg gacaaagaag
4801 aagacgttca ggtcctcgcc atagaaccag gaaaaaatcc taaacatgtc caaacgaaac
4861 ctggcctttt caagacccta actggagaaa ttggagcagt aacattagat ttcaaacccg
4921 gaacgtctgg ttctcccatc atcaacagga aaggaaaagt catcggactc tatggaaatg
4981 gagtagttac caaatcaggt gattacgtca gtgccataac gcaagccgaa agaattggag
5041 agccagatta tgaagtggat gaggacattt ttcgaaagaa aagattaact ataatggact
5101 tacaccccgg agctggaaag acaaaaagaa ttcttccatc aatagtgaga gaagccttaa
5161 aaaggaggct acgaactttg attttagctc ccacgagagt ggtggcggcc gagatggaag
5221 aggccctacg tggactgcca atccgttatc agaccccagc tgtgaaatca gaacacacag
5281 gaagagagat tgtagacctc atgtgtcatg caaccttcac aacaagactt ttgtcatcaa
5341 ccagggttcc aaattacaac cttatagtga tggatgaagc acatttcacc gatccttcta
5401 gtgtcgcggc tagaggatac atctcgacca gggtggaaat gggagaggca gcagccatct
5461 tcatgaccgc aacccctccc ggagcgacag atccctttcc ccagagcaac agcccaatag
5521 aagacatcga gagggaaatt ccggaaaggt catggaacac agggttcgac tggataacag
5581 actaccaagg gaaaactgtg tggtttgttc ccagcataaa agctggaaat gacattgcaa
5641 attgtttgag aaagtcggga aagaaagtta tccagttgag taggaaaacc tttgatacag
5701 agtatccaaa aacgaaactc acggactggg actttgtggt cactacagac atatctgaaa
5761 tgggggccaa ttttagagcc gggagagtga tagaccctag aagatgcctc aagccagtta
5821 tcctaccaga tgggccagag agagtcattt tagcaggtcc tattccagtg actccagcaa
5881 gcgctgctca gagaagaggg cgaataggaa ggaacccagc acaagaagac gaccaatacg
5941 ttttctccgg agacccacta aaaaatgatg aagatcatgc ccactggaca gaagcaaaga
6001 tgctgcttga caatatctac accccagaag ggatcattcc aacattgttt ggtccggaaa
6061 gggaaaaaac ccaagccatt gatggagagt ttcgcctcag aggggaacaa aggaagactt
6121 ttgtggaatt aatgaggaga ggagaccttc cggtgtggct gagctataag gtagcttctg
6181 ctggcatttc ttacgaagat cgggaatggt gcttcacagg ggaaagaaat aaccaaattt
6241 tagaagaaaa catggaggtt gaaatttgga ctagagaggg agaaaagaaa aagctaaggc
6301 caagatggtt agatgcacgt gtatacgctg accccatggc tttgaaggat ttcaaggagt
6361 ttgccagtgg aaggaagagt ataactctcg acatcctaac agagattgcc agtttgccaa
6421 cttacctttc ctctagggcc aagctcgccc ttgataacat agtcatgctc cacacaacag
6481 aaagaggagg gagggcctat caacacgccc tgaacgaact tccggagtca ctggaaacac
6541 tcatgcttgt agctttacta ggtgctatga cagcaggcat cttcctgttt ttcatgcaag
6601 ggaaaggaat agggaaattg tcaatgggtt tgataaccat tgcggtggct agtggcttgc
6661 tctgggtagc agaaattcaa ccccagtgga tagcggcctc aatcatacta gagttttttc
6721 tcatggtact gttgataccg gaaccagaaa aacaaaggac cccacaagac aatcaattga
6781 tctacgtcat attgaccatt ctcaccatca ttggtctaat agcagccaac gagatggggc
6841 tgattgaaaa aacaaaaacg gattttgggt tttaccaggt aaaaacagaa accaccatcc
6901 tcgatgtgga cttgagacca gcttcagcat ggacgctcta tgcagtagcc accacaattc
6961 tgactcccat gctgagacac accatagaaa acacgtcggc caacctatct ctagcagcca
7021 ttgccaacca ggcagccgtc ctaatggggc ttggaaaagg atggccgctc cacagaatgg
7081 acctcggtgt gccgctgtta gcaatgggat gctattctca agtgaaccca acaaccttga
7141 cagcatcctt agtcatgctt ttagtccatt atgcaataat aggcccagga ttgcaggcaa
7201 aagccacaag agaggcccag aaaaggacag ctgctgggat catgaaaaat cccacagtgg
7261 acgggataac agtaatagat ctagaaccaa tatcctatga cccaaaattt gaaaagcaat
7321 tagggcaggt catgctacta gtcttgtgtg ctggacaact actcttgatg agaacaacat
7381 gggctttctg tgaagtcttg actttggcca caggaccaat cttgaccttg tgggagggca
7441 acccgggaag gttttggaac acgaccatag ccgtatccac cgccaacatt ttcaggggaa
7501 gttacttggc gggagctgga ctggcttttt cactcataaa gaatgcacaa acccctagga
7561 ggggaactgg gaccacagga gagacactgg gagagaagtg gaagagacag ctaaactcat
7621 tagacagaaa agagtttgaa gagtataaaa gaagtggaat actagaagtg gacaggactg
7681 aagccaagtc tgccctgaaa gatgggtcta aaatcaagca tgcagtatca agagggtcca
7741 gtaagatcag atggattgtt gagagaggga tggtaaagcc aaaagggaaa gttgtagatc
7801 ttggctgtgg gagaggagga tggtcttatt acatggcgac actcaagaac gtgactgaag
7861 tgaaagggta tacaaaagga ggtccaggac atgaagaacc gattcccatg gctacttatg
7921 gttggaattt ggtcaaactc cattcagggg ttgacgtgtt ctacaaaccc acagagcaag
7981 tggacaccct gctctgtgat attggggagt catcttctaa tccaacaata gaggaaggaa
8041 gaacattaag agttttgaag atggtggagc catggctctc ttcaaaacct gaattctgca
8101 tcaaagtcct taacccctac atgccaacag tcatagaaga gctggagaaa ctgcagagaa
8161 aacatggtgg gaaccttgtc agatgcccgc tgtccaggaa ctccacccat gagatgtatt
8221 gggtgtcagg agcgtcggga aacattgtga gctctgtgaa cacaacatca aagatgttgt
8281 tgaacaggtt cacaacaagg cataggaaac ccacttatga gaaggacgta gatcttgggg
8341 caggaacgag aagtgtctcc actgaaacag aaaaaccaga catgacaatc attgggagaa
8401 ggcttcagcg attgcaagaa gagcacaaag aaacctggca ttatgatcag gaaaacccat
8461 acagaacctg ggcgtatcat ggaagctatg aagctccttc gacaggctct gcatcctcca
8521 tggtgaacgg ggtggtaaaa ctgctaacaa aaccctggga tgtgattcca atggtgactc
8581 agttagccat gacagataca accccttttg ggcaacaaag agtgttcaaa gagaaggtgg
8641 ataccagaac accacaacca aaacccggta cacgaatggt tatgaccacg acagccaatt
8701 ggctgtgggc cctccttgga aagaagaaaa atcccagact gtgcacaagg gaagagttca
8761 tctcaaaagt tagatcaaac gcagccatag gcgcagtctt tcaggaagaa cagggatgga
8821 catcagccag tgaagctgtg aatgacagcc ggttttggga actggttgac aaagaaaggg
8881 ccctacacca ggaagggaaa tgtgaatcgt gtgtctataa catgatggga aaacgtgaga
8941 aaaagttagg agagtttggc agagccaagg gaagccgagc aatctggtac atgtggctgg
9001 gagcgcggtt tctggaattt gaagccctgg gttttttgaa tgaagatcac tggtttggca
9061 gagaaaattc atggagtgga gtggaagggg aaggtctgca cagattggga tatatcctgg
9121 aggagataga caagaaggat ggagacctaa tgtatgctga tgacacagca ggctgggaca
9181 caagaatcac tgaggatgac cttcaaaatg aggaactgat cacggaacag atggctcccc
9241 accacaagat cctagccaaa gccattttca aactaaccta tcaaaacaaa gtggtgaaag
9301 tcctcagacc cacaccgcgg ggagcggtga tggatatcat atccaggaaa gaccaaagag
9361 gtagtggaca agttggaaca tatggtttga acacattcac caacatggaa gttcaactca
9421 tccgccaaat ggaagctgaa ggagtcatca cacaagatga catgcagaac ccaaaagggt
9481 tgaaagaaag agttgagaaa tggctgaaag agtgtggtgt cgacaggtta aagaggatgg
9541 caatcagtgg agacgattgc gtggtgaagc ccctagatga gaggtttggc acttccctcc
9601 tcttcttgaa cgacatggga aaggtgagga aagacattcc gcagtgggaa ccatctaagg
9661 gatggaaaaa ctggcaagag gttccttttt gctcccacca ctttcacaag atctttatga
9721 aggatggccg ctcactagtt gttccatgta gaaaccagga tgaactgata gggagagcca
9781 gaatctcgca gggagctgga tggagcttaa gagaaacagc ctgcctgggc aaagcttacg
9841 cccagatgtg gtcgcttatg tacttccaca gaagggatct gcgtttagcc tccatggcca
9901 tatgctcagc agttccaacg gaatggtttc caacaagcag aacaacatgg tcaatccacg
9961 ctcatcacca gtggatgacc actgaagata tgctcaaagt gtggaacaga gtgtggatag
10021 aagacaaccc taatatgact gacaagactc cagtccattc gtgggaagat ataccttacc
10081 tagggaaaag agaggatttg tggtgtggat ccctgattgg actttcttcc agagccacct
10141 gggcgaagaa cattcatacg gccataaccc aggtcaggaa cctgatcgga aaagaggaat
10201 acgtggatta catgccagta atgaaaagat acagtgctcc ttcagagagt gaaggagttc
10261 tgtaattacc aacaacaaac accaaaggct attgaagtca ggccacttgt gccacggttt
10321 gagcaaaccg tgctgcctgt agctccgcca ataatgggag gcgtaataat ccccagggag
10381 gccatgcgcc acggaagctg tacgcgtggc atattggact agcggttaga ggagacccct
10441 cccatcactg ataaaacgca gcaaaagggg gcccgaagcc aggaggaagc tgtactcctg
10501 gtggaaggac tagaggttag aggagacccc cccaacacaa aaacagcata ttgacgctgg
10561 gaaagaccag agatcctgct gtctctgcaa catcaatcca ggcacagagc gccgcaagat
10621 ggattggtgt tgttgatcca acaggttct
//
