LOCUS NC_003930 11919 bp RNA linear VRL 13-AUG-2018
DEFINITION Salmon pancreas disease virus, complete genome.
ACCESSION NC_003930
VERSION NC_003930.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq; 6K gene; 6K protein; capside protein; complete genome; cp
gene; E1 gene; E1 protein; E2 gene; E2 protein; E3 gene; E3
protein; non structural protein P1; non structural protein P2; non
structural protein P3; non structural protein P4; nonstructural
polyprotein; nsp1 gene; NSP2 gene; NSP3 gene; nsp4 gene; structural
polyprotein.
SOURCE Salmon pancreas disease virus (SPDV)
ORGANISM Salmon pancreas disease virus
Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
Martellivirales; Togaviridae; Alphavirus.
REFERENCE 1
AUTHORS Weston,J.H., Villoing,S., Bremont,M., Castric,J., Pfeffer,M.,
Jewhurst,V., McLoughlin,M., Rodseth,O., Christie,K.E., Koumans,J.
and Todd,D.
TITLE Comparison of two aquatic alphaviruses, salmon pancreas disease
virus and sleeping disease virus, by using genome sequence
analysis, monoclonal reactivity, and cross-infection
JOURNAL J. Virol. 76 (12), 6155-6163 (2002)
PUBMED 12021349
REFERENCE 2 (bases 1 to 11919)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (04-JUN-2002) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 11919)
AUTHORS Weston,J.H.
TITLE Direct Submission
JOURNAL Submitted (06-SEP-2001) Weston J.H., Veterinary Sciences Division,
The Queen's University of Belfast, Stoney Road, Belfast, BT4 3SD,
UNITED KINGDOM
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to AJ316244.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..11919
/organism="Salmon pancreas disease virus"
/mol_type="genomic RNA"
/isolate="F93125"
/db_xref="taxon:84589"
/cell_line="CHSE-214 cell line"
/country="Ireland"
5'UTR 1..27
gene 28..7833
/locus_tag="SPDVgp01"
/db_xref="GeneID:2193531"
CDS 28..7833
/locus_tag="SPDVgp01"
/codon_start=1
/product="non-structural polyprotein"
/protein_id="NP_647496.1"
/db_xref="GOA:Q8JJX1"
/db_xref="InterPro:IPR000606"
/db_xref="InterPro:IPR001788"
/db_xref="InterPro:IPR002589"
/db_xref="InterPro:IPR002620"
/db_xref="InterPro:IPR002877"
/db_xref="InterPro:IPR007094"
/db_xref="UniProtKB/Swiss-Prot:Q8JJX1"
/db_xref="GeneID:2193531"
/translation="MMQNLTANPSAGATVTVNLPADHPALNQFKTAFPGFEVVASNRS
SNDHAAARAFSHLATKWIERDIGGRQVIVADIGSAPARRIGAPDNVTYHSVCPRKCAE
DPERLASYARKLVRAVERGDGHLVNEKITDLKDVLENPDTSLETTSICLNDDVSCKVK
ADIAVYQDVYAVDAPSTIYAQADKGTRVVYWIGFEPFVFHTDAMAGSFPLYDANWSDS
AVLAAKNLPLCYSGLSEDSIKWRFRFRDKPLVPSGEIHYSVGSTHYVEDRDKLKSWHL
PSTFHFVAPNKYTCRCDTVVSCGGYVVKKITICEGIVGIPAKEELATSYHRDGVVVTK
FSDTINHEQVSFPVVTYIPAVICDQMTAMTANPVKYSDAVKLLVGLNQRIVVNGTTVR
NVNSMDNSLIPVFARALCSWADEVRRDMEDEQDLYGITSVTTWICICRAYDKRQQHTF
YRRPKQSSGIYVPAKFTGSLRAALSATYLNLPLKQLLLNTLKRAIKPMDQAIADETEA
LAHDAAEVHELTEEERRQQAANPSYIADVLGQDDDEEEAGDGMSDVDLGEEDGAGATI
IDCQRGTVKVITAFGDNMMGEYLVLSPVTVLRTRKLAILLGPLAEEVMQYVHKGRTGR
YAIEKNNLKVLIPTGVSLKTDHFQALAESATLTYNDYLFTCRTLDQLATRGSARNTDE
VYYKLVDAAKARDEYVYELSSKQCVKKEDATGTVLQGDICNPPYHQFAYEALRKRPAH
THDVHTIGIYGVPGAGKTAIITTEVTTRDLVASGKKENCEDIKRCVLERRGLKIAART
VDSLFYGAYRGAVNTLYVDEAYACHSGTLLALIAAVRPTGKVVLCGDPKQVGCVNQLQ
MRMHYNHEISDRVLRKNISRRCTHTLTAIVSNLNYEGRMKTTNPCKKPVLIDTTGSTK
PDKEALVLTCFRGWVKDLKFLYPHNELMTAAASQGLTREKVYAVRCRVTTNPLYEPTS
EHITVLLTRTNDELVWKTLPNDPLIPILSKPPKGDYSATMEDWEDEHNGILAALREAC
VPRMNFAHGKRNTCWAVTSSRVLHEAGVQITPEDYNRIFPAFREDKPHSALAALDAVA
TLVWGLDTSSGILSGKGSFMRLENSHWSNSNRGYEYGLNLDALEGYEIANPRMIKALK
QRRGRECYDIETGKLVPLDPARVQVPINRIVPHVLVDTSAAAKPGFLENRLTVDRWDQ
VHSFKTRAAVKFAELTKRVSYNSVLDLGAAPGGVTDYCVKKGKTVTSVSEQWDTKPRG
AVVVTADINGPLNNLGIFDLVFCDAAGPRRYHHYAQCEDHAVLFTSACKHGVERTAKG
GVFIVKAYGMADRRTERAVEGTARYFRSVSVEKPVSSRITNVEVFFKFSGRCRPHARS
IAHLGPQLTDIYARTWKAYKMLARGSVADKVKVAEILNSMVGAAPGYRVLNRNIITAE
EEVLVNAANSNGRPGDGVCGALYGAFGDAFPNGAIGAGNAVLVRGLEATIIHAAGADF
REVDEETGARQLRAAYRAAATLVTANGITSAAIPLLSTHIFSNGRNRLEQSFSALVEA
FDTTECDVTIYCLANNMAARIQQLIDAHAREEFDEEVVVEEEEEHEADAMSDTETLSS
FGDETVWVPKHSTLAGRPGYSAYYGDRRSLFVGTKFHRAAVAMSSIEAAWPKTKEANA
KLIEYIRGQHLVDVLKSCPVDDIPVGRPPSSLPCGCIYAMTPERVTVLKQRPQEGFVV
CSAFKLPLTNIQDVTKVECTVRAPAEEPRPVRHLQERRPAQAAVRQLRPAAVAASVAA
SHTASRTSTASSRRTPAPGSVQVRLLPPRDGTESRSSRMGSQSSVTSSAGSVPPAPRR
APAVSAASLASSAHSRSVRSAPAMRAASAGARSVRSAQSGSTGHRAGAFSVAGSVRQP
SGPPSSVSTPAAIRGLTRDQFDAVRVRARRNLELEGSEHGSQSSFHSGSLAVGSSASS
YSQRSDDQDTGTEPSSRGAAVRTRRRGQRDGLGGYIFSSDQGTAHLSQHNTQTNNTTE
VLMRTSVLPSNDHGTPDLPAETRKRLAYQMRPTQKNKSRYLSAKVHNMKHKIVRCLQR
GAGHYLREQHALPLWKNTFPKPRYSDACVVKFESVNTAIVAANMFIGCNYPTLSSFGI
TDKYDAYLDMVDGLNCNLDTVTFDPAKVRSLPKKSEYNQPLIQSQVPGPMTSTLQSIL
MAATKRNCNVTQMRELPTMDSAAMNVEAFKSFACKDTDLWTEFAEKPVRLSPGQIEEY
VFHLQGAKANVMHSRVEAVCPDLSEVAMDRFTLDMKRDVKVTPGTKHVEERPKVQEIQ
AADPMATAYLCAIHRELVRRLKAVLKPSIHVLFDMSSEDFDAIVGHGMKLGDKVLETD
ISSFDKSQDQAMAVTALMLLRDLGVEEDLLTLIEASFGDITSAHLPTGTRFQFGSMMK
SGLFLTLFVNTLLNITIAARVLREQLADTRCAAFIGDDNVITGVVSDDMMVARCASWL
NMEVKIMDMEIGNMSPYFCGGFLLLDTVTGTVSRVSDPVKRLMKMGKPALNDPETDVD
RCRALREEVESWYRVGIQWPLQVAAATRYGVNHLPLATMAMATLAQDLRSYLGARGEY
VSLYV"
mat_peptide 28..1713
/locus_tag="SPDVgp01"
/product="non structural protein P1"
/protein_id="NP_740635.1"
misc_feature 115..1191
/locus_tag="SPDVgp01"
/note="Viral methyltransferase; Region: Vmethyltransf;
cl46422"
/db_xref="CDD:480762"
mat_peptide 1714..4290
/locus_tag="SPDVgp01"
/product="non structural protein P2"
/protein_id="NP_740636.1"
misc_feature 2272..2988
/locus_tag="SPDVgp01"
/note="Viral (Superfamily 1) RNA helicase; Region:
Viral_helicase1; pfam01443"
/db_xref="CDD:366646"
misc_feature 3649..4125
/locus_tag="SPDVgp01"
/note="FtsJ-like methyltransferase; Region: FtsJ;
pfam01728"
/db_xref="CDD:426399"
mat_peptide 4291..6003
/locus_tag="SPDVgp01"
/product="non structural protein P3"
/protein_id="NP_740637.1"
misc_feature 4336..4719
/locus_tag="SPDVgp01"
/note="X-domain (or Mac1 domain) of viral non-structural
protein 3 and related macrodomains; Region:
Macro_X_Nsp3-like; cd21557"
/db_xref="CDD:438957"
misc_feature order(4354..4362,4372..4392,4609..4614,4618..4632,
4714..4716)
/locus_tag="SPDVgp01"
/note="ADP-ribose binding site [chemical binding]; other
site"
/db_xref="CDD:438957"
misc_feature 5269..>5994
/locus_tag="SPDVgp01"
/note="transcriptional regulator ICP4; Provisional;
Region: PHA03307"
/db_xref="CDD:223039"
mat_peptide 6004..7830
/locus_tag="SPDVgp01"
/product="non structural protein P4"
/protein_id="NP_740638.1"
misc_feature 6451..7827
/locus_tag="SPDVgp01"
/note="conserved catalytic core domain of RNA-dependent
RNA polymerase (RdRp) from the positive-sense
single-stranded RNA [(+)ssRNA] viruses and closely related
viruses; Region: ps-ssRNAv_RdRp-like; cl40470"
/db_xref="CDD:477363"
misc_feature 7102..7146
/locus_tag="SPDVgp01"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438100"
misc_feature order(7288..7338,7342..7359)
/locus_tag="SPDVgp01"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438100"
misc_feature order(7381..7392,7396..7425)
/locus_tag="SPDVgp01"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438100"
gene 28..1713
/gene="nsP1"
/locus_tag="SPDVgp02"
/db_xref="GeneID:944549"
gene 1714..4290
/gene="nsP2"
/locus_tag="SPDVgp03"
/db_xref="GeneID:944545"
gene 4291..6003
/gene="nsP3"
/locus_tag="SPDVgp04"
/db_xref="GeneID:944548"
gene 6004..7830
/gene="nsP4"
/locus_tag="SPDVgp05"
/db_xref="GeneID:944544"
gene 7869..11831
/locus_tag="SPDVgp06"
/db_xref="GeneID:2193532"
CDS 7869..11831
/locus_tag="SPDVgp06"
/codon_start=1
/product="structural polyprotein"
/protein_id="NP_647497.1"
/db_xref="GOA:Q8JJX0"
/db_xref="InterPro:IPR000930"
/db_xref="InterPro:IPR000936"
/db_xref="InterPro:IPR002533"
/db_xref="InterPro:IPR002548"
/db_xref="InterPro:IPR009003"
/db_xref="InterPro:IPR011998"
/db_xref="InterPro:IPR014756"
/db_xref="UniProtKB/Swiss-Prot:Q8JJX0"
/db_xref="GeneID:2193532"
/translation="MFPMQFTNSAYRQMEPMFAPGSRGQVQPYRPRTKRRQEPQVGNA
AITALANQMSALQLQVAGLAGQARVDRRGPRRVQKNKQKKKNSSNGEKPKEKKKKQKQ
QEKKGSGGEKVKKTRNRPGKEVRISVKCARQSTFPVYHEGAISGYAVLIGSRVFKPAH
VKGKIDHPELADIKFQVAEDMDLEAAAYPKSMRDQAAEPATMMDRVYNWEYGTIRVED
NVIIDASGRGKPGDSGRAITDNSGKVVGIVLGGGPDGRRTRLSVIGFDKKMKAREIAY
SDAIPWTRAPALLLLPMVIVCTYNSNTFDCSKPSCQDCCITAEPEKAMTMLKDNLNDP
NYWDLLIAVTTCGSARRKRAVSTSPAAFYDTQILAAHAAASPYRAYCPDCDGTACISP
IAIDEVVSSGSDHVLRMRVGSQSGVTAKGGAAGETSLRYLGRDGKVHAADNTRLVVRT
TAKCDVLQATGHYILANCPVGQSLTVAATLDGTRHQCTTVFEHQVTEKFTRERSKGHH
LSDMTKKCTRFSTTPKKSALYLVDVYDALPISVEISTVVTCSDSQCTVRVPPGTTVKF
DKKCKSADSATVTFTSDSQTFTCEEPVLTAASITQGKPHLRSAMLPSGGKEVKARIPF
PFPPETATCRVSVAPLPSITYEESDVLLAGTAKYPVLLTTRNLGFHSNATSEWIQGKY
LRRIPVTPQGIELTWGNNAPMHFWSSVRYASGDADAYPWELLVYHTKHHPEYAWAFVG
VACGLLAIAACMFACACSRVRYSLVANTFNSNPPPLTALTAALCCIPGARADQPYLDI
IAYLWTNSKVAFGLQFAAPVACVLIITYALRHCRLCCKSFLGVRGWSALLVILAYVQS
CKSYEHTVVVPMDPRAPSYEAVINRNGYDPLKLTISVNFTVISPTTALEYWTCAGVPI
VEPPHVGCCTSVSCPSDLSTLHAFTGKAVSDVHCDVHTNVYPLLWGAAHCFCSTENTQ
VSAVAATVSEFCAQDSERAEAFSVHSSSVTAEVLVTLGEVVTAVHVYVDGVTSARGTD
LKIVAGPITTDYSPFDRKVVRIGEEVYNYDWPPYGAGRPGTFGDIQARSTNYVKPNDL
YGDIGIEVLQPTNDHVHVAYTYTTSGLLRWLQDAPKPLSVTAPHGCKISANPLLALDC
GVGAVPMSINIPDAKFTRKLKDPKPSALKCVVDSCEYGVDYGGAATITYEGHEAGKCG
IHSLTPGVPLRTSVVEVVAGANTVKTTFSSPTPEVALEVEICSAIVKCAGECTPPKEH
VVATRPRHGSDPGGYISGPAMRWAGGIVGTLVVLFLILAVIYCVVKKCRSKRIRIVKS
"
mat_peptide 7869..8714
/locus_tag="SPDVgp06"
/product="capside protein"
/protein_id="NP_740639.1"
misc_feature 8274..8684
/locus_tag="SPDVgp06"
/note="Alphavirus core protein; Region: Peptidase_S3;
pfam00944"
/db_xref="CDD:366379"
mat_peptide 8715..8927
/locus_tag="SPDVgp06"
/product="E3 protein"
/protein_id="NP_740640.1"
mat_peptide 8928..10241
/locus_tag="SPDVgp06"
/product="E2 protein"
/protein_id="NP_740641.1"
misc_feature 10227..11786
/locus_tag="SPDVgp06"
/note="Alphavirus E1 glycoprotein; Region:
Alpha_E1_glycop; pfam01589"
/db_xref="CDD:279870"
mat_peptide 10242..10445
/locus_tag="SPDVgp06"
/product="6K protein"
/protein_id="NP_740642.1"
mat_peptide 10446..11828
/locus_tag="SPDVgp06"
/product="E1 protein"
/protein_id="NP_740643.1"
gene 7869..10522
/locus_tag="SPDVgp12"
/db_xref="GeneID:13165427"
CDS join(7869..10385,10385..10522)
/locus_tag="SPDVgp12"
/note="Truncated verstion of structural polyprotein that
will be produced when frameshifting occurs at nt 10385;
Truncated version of structural polyprotein and transframe
fusion protein have been added by NCBI Staff with the kind
help of Dr. David Karlin (Department of Zoology,
University of Oxford, UK) and Dr. Andrew Firth (Department
of Pathology, University of Cambridge, UK)"
/codon_start=1
/product="truncated polyprotein"
/protein_id="YP_006491239.1"
/db_xref="GeneID:13165427"
/translation="MFPMQFTNSAYRQMEPMFAPGSRGQVQPYRPRTKRRQEPQVGNA
AITALANQMSALQLQVAGLAGQARVDRRGPRRVQKNKQKKKNSSNGEKPKEKKKKQKQ
QEKKGSGGEKVKKTRNRPGKEVRISVKCARQSTFPVYHEGAISGYAVLIGSRVFKPAH
VKGKIDHPELADIKFQVAEDMDLEAAAYPKSMRDQAAEPATMMDRVYNWEYGTIRVED
NVIIDASGRGKPGDSGRAITDNSGKVVGIVLGGGPDGRRTRLSVIGFDKKMKAREIAY
SDAIPWTRAPALLLLPMVIVCTYNSNTFDCSKPSCQDCCITAEPEKAMTMLKDNLNDP
NYWDLLIAVTTCGSARRKRAVSTSPAAFYDTQILAAHAAASPYRAYCPDCDGTACISP
IAIDEVVSSGSDHVLRMRVGSQSGVTAKGGAAGETSLRYLGRDGKVHAADNTRLVVRT
TAKCDVLQATGHYILANCPVGQSLTVAATLDGTRHQCTTVFEHQVTEKFTRERSKGHH
LSDMTKKCTRFSTTPKKSALYLVDVYDALPISVEISTVVTCSDSQCTVRVPPGTTVKF
DKKCKSADSATVTFTSDSQTFTCEEPVLTAASITQGKPHLRSAMLPSGGKEVKARIPF
PFPPETATCRVSVAPLPSITYEESDVLLAGTAKYPVLLTTRNLGFHSNATSEWIQGKY
LRRIPVTPQGIELTWGNNAPMHFWSSVRYASGDADAYPWELLVYHTKHHPEYAWAFVG
VACGLLAIAACMFACACSRVRYSLVANTFNSNPPPLTALTAALCCIPGARADQPYLDI
IAYLWTNSKVAFGLQFAAPVACVLIITYALRHCRLCCKSFLRGKRVVSPAGHPCVCTE
LQELRTHRGGPNGSKSPVVRSSDKPEWV"
misc_feature 8274..8684
/locus_tag="SPDVgp12"
/note="Alphavirus core protein; Region: Peptidase_S3;
pfam00944"
/db_xref="CDD:366379"
misc_feature 10227..>10382
/locus_tag="SPDVgp12"
/note="Alphavirus E1 glycoprotein; Region:
Alpha_E1_glycop; pfam01589"
/db_xref="CDD:279870"
mat_peptide join(10242..10385,10385..10519)
/locus_tag="SPDVgp12"
/product="transframe fusion protein"
/note="Transframe fusion (TF) protein expressed via
programmed ribosomal frameshifting. TF protein presumably
plays a stabilizing role in the virion structure."
/protein_id="YP_006491240.1"
gene 7869..8714
/gene="cp"
/locus_tag="SPDVgp07"
/db_xref="GeneID:944551"
gene 8715..8927
/gene="E3"
/locus_tag="SPDVgp08"
/db_xref="GeneID:944550"
gene 8928..10241
/gene="E2"
/locus_tag="SPDVgp09"
/db_xref="GeneID:944552"
gene 10242..10445
/gene="6K"
/locus_tag="SPDVgp10"
/db_xref="GeneID:944546"
gene 10446..11828
/gene="E1"
/locus_tag="SPDVgp11"
/db_xref="GeneID:944547"
3'UTR 11832..11919
ORIGIN
1 nttaaatcca gaagcataca tatatcaatg atgcaaaatc tcacagctaa cccctccgcc
61 ggcgctacag ttactgtaaa tctgcccgcg gaccacccgg ccctgaacca gttcaagact
121 gcgtttccag ggttcgaagt ggtggccagc aataggtcgt ctaacgacca tgccgccgcc
181 agagctttct cccacttggc taccaagtgg attgagcgcg acatcggtgg ccgtcaggtc
241 atcgttgccg acatagggag cgcaccagcg agaagaatcg gggcacccga taacgtcaca
301 taccacagtg tgtgtcctcg caaatgcgct gaagaccctg agaggctggc ttcttatgcc
361 aggaaactcg tcagggctgt ggagagagga gatggacacc tcgtcaatga aaaaatcact
421 gacttgaagg atgttctgga gaacccggac acgtcactgg aaaccaccag tatctgcctg
481 aacgacgacg tgagttgtaa agtaaaggcc gatattgcag tgtaccagga tgtgtacgca
541 gtcgatgctc cgtctaccat ctacgcgcag gccgacaaag gtacgcgagt ggtatactgg
601 atcgggttcg agccgttcgt gttccacacg gacgctatgg cgggtagctt cccgctatat
661 gacgccaact ggagcgactc ggcggtactg gcagctaaaa acctgccact gtgctactca
721 gggttgtcgg aggattccat taaatggaga ttccgtttcc gtgacaagcc actggtaccg
781 tctggagaaa tccactactc ggtgggtagc acccactacg ttgaggaccg tgacaagctg
841 aaaagctggc acctgccttc taccttccac ttcgtggcac ccaacaagta tacatgccgt
901 tgcgatacgg tggtaagctg tggcggctac gtcgtaaaga agatcacgat ctgtgagggt
961 atcgtcggta ttcccgcgaa ggaggaactg gccacctcgt accaccgtga tggagtggtg
1021 gtgaccaagt tctctgatac gatcaaccac gaacaggtgt cattcccagt ggtgacctac
1081 atccccgccg tcatctgtga tcagatgacc gccatgacgg ccaatcccgt caaatactcg
1141 gatgccgtca aactgctggt aggactcaac caaaggatcg tggtcaacgg gaccaccgtc
1201 cgcaacgtca actccatgga taactcgctg attcccgtat tcgctcgcgc attatgcagc
1261 tgggcggacg aagttcggcg ggacatggag gacgaacaag acctgtacgg tatcacgtcc
1321 gtcaccacat ggatctgtat ctgcagagcg tatgacaaac gccagcagca taccttctac
1381 cgtaggccca agcagtcctc gggcatatat gtgcctgcca agttcacagg gtcgctgaga
1441 gccgccctct cagccaccta ccttaacctg cctctgaagc aactgctgct taatacgctc
1501 aaacgtgcga ttaaacctat ggatcaggcc atcgcagatg agacagaagc acttgcgcac
1561 gacgcagctg aggtgcacga gttgacagag gaggagcgca ggcaacaggc cgccaatcct
1621 agttacatcg cagacgtgct gggccaagac gacgacgagg aggaagccgg cgacggcatg
1681 tctgatgtgg acctcggcga agaagacggg gcgggtgcca ccatcatcga ttgtcaaaga
1741 ggcaccgtga aagtaatcac ggcgttcggt gacaacatga tgggggaata cctagtattg
1801 tccccggtga ccgtgttgcg caccaggaaa ctggccattc tgctcggacc actcgcggaa
1861 gaagtgatgc agtatgtcca caaaggccgg accgggcgct atgccattga gaagaataac
1921 ctgaaggtac tcatccctac gggggtgtcc ctcaagaccg atcacttcca ggcccttgcc
1981 gaaagtgcta ccttgaccta caacgactac ctgttcacat gccgcacgct cgaccagctg
2041 gcaacaagag gatcagcaag aaacaccgac gaagtctact acaagcttgt tgacgccgct
2101 aaggcgaggg acgaatacgt ttacgaactg tcctccaagc aatgcgttaa gaaagaagat
2161 gcaacaggca cagtcctcca aggggacata tgcaatccgc cttatcacca gtttgcctac
2221 gaggcgctgc gtaaaaggcc ggctcacaca cacgacgtcc acacgattgg catctacggg
2281 gttccgggtg caggaaaaac cgctatcatc acaaccgaag taactacacg cgacttggtc
2341 gctagtggaa aaaaggagaa ttgtgaagac atcaagcggt gcgtgttgga gagacgcggc
2401 ttgaaaatag ccgcacgtac ggttgactca ctgttctacg gtgcataccg aggagcggtc
2461 aatacgctgt acgttgacga ggcttacgcc tgtcactcgg gcactctgct cgccttgatc
2521 gctgcggtaa gaccgacggg aaaggtggtg ctttgtggtg accctaaaca agtcggatgt
2581 gtgaaccagc tccaaatgcg gatgcattac aaccatgaga ttagtgaccg cgttttgcgg
2641 aagaacatct cccgccgttg cactcatacg cttacggcca tcgtttcaaa cctcaactac
2701 gaaggtagaa tgaagactac gaacccgtgc aaaaaacccg tactaattga caccaccgga
2761 tccaccaagc ccgacaagga agcgttggtg ttgacgtgct tccgcgggtg ggtcaaggat
2821 ctaaaattcc tctatcctca caacgagctc atgactgcgg ccgcttcgca gggcctgacc
2881 cgcgaaaagg tgtatgccgt tcgttgtcgt gtcacgacga acccactcta tgagccgacc
2941 tctgagcaca ttactgtcct tttgacgcgc accaacgacg aactggtatg gaagacactg
3001 ccaaacgacc cgctgatccc tatactctcc aagccaccga aaggagacta ctccgctacc
3061 atggaggatt gggaggatga acacaacggt atcctggctg ccctcagaga agcgtgtgtc
3121 ccacggatga atttcgcgca tgggaagcgt aatacctgtt gggcagttac gagcagccgg
3181 gtgctgcatg aggcgggcgt ccagataacg ccggaggact ataaccgtat ctttccggcg
3241 tttcgagagg acaagccgca ttcggctctg gcagccttgg atgccgtcgc tactctcgtg
3301 tggggcttgg atacttcctc gggcattctg agtggaaaag gcagcttcat gcggctggag
3361 aacagccact ggagtaactc caacaggggg tacgagtacg gcctcaacct ggacgcactg
3421 gaaggctatg agattgccaa tccacgcatg attaaagcgc ttaaacaacg gcgcgggcgc
3481 gaatgttacg acatcgaaac ggggaagttg gtgcccctgg atcctgccag ggtccaagtg
3541 ccgattaacc gaatcgtgcc ccacgtgttg gtggacactt ctgcagcggc caaaccaggc
3601 ttcctggaaa acagactcac agttgacaga tgggaccaag tgcacagctt caagacaaga
3661 gcagcagtca agtttgcaga actgacgaag cgtgtctcat ataactcagt ccttgacctt
3721 ggagccgccc cgggaggagt gaccgactac tgcgtgaaga aaggaaaaac cgtcacaagc
3781 gtctccgagc agtgggatac taagccgaga ggcgctgtgg tcgttacggc agatatcaac
3841 ggcccactta acaacctagg tatcttcgac ctagtgtttt gtgacgcagc tggaccacga
3901 cgctatcacc actacgcgca gtgcgaagac cacgccgtgc tgttcacatc agcatgcaaa
3961 catggggtgg agcgcacagc caagggcgga gtcttcattg tgaaggcgta cggaatggcg
4021 gaccgccgaa cagagagggc agtggaaggt acagcgaggt atttcaggtc cgtttcggtc
4081 gaaaagcccg tctcctcccg tataaccaac gtggaagtct tcttcaagtt ctctggcaga
4141 tgtcgcccac acgcacgttc catcgcacat ttgggccctc aactgaccga catttacgcc
4201 cgtacgtgga aggcgtataa aatgctggcg agaggcagtg tcgctgacaa ggtgaaagtg
4261 gcagagatac ttaactcaat ggtaggtgcc gcaccggggt acagagtcct caacaggaac
4321 atcatcactg ccgaagagga agttctggtc aacgccgcca acagcaacgg tagacccggt
4381 gacggtgtgt gtggtgcgct ctacggcgcg tttggggacg cgttccccaa cggtgcaatc
4441 ggcgcgggaa acgcggtcct ggttcgagga ctcgaggcta ccatcataca cgcagccgga
4501 gctgacttca gagaggttga cgaagaaacc ggcgcgcggc agctaagagc agcataccgc
4561 gcggcggcta ccctagtcac ggccaacggc atcaccagcg ccgccatccc cttgctgagt
4621 acacacatct tctctaacgg tcggaacagg ctggaacagt cgtttagtgc actggtggag
4681 gcgttcgaca cgacagagtg cgacgtcacc atctactgcc tcgccaacaa catggccgcg
4741 aggatccagc aactgattga cgcccacgct cgcgaagagt ttgatgagga agtggttgtg
4801 gaagaggaag aggaacacga agccgacgca atgagtgaca cggagacgct gtccagtttc
4861 ggcgacgaaa cggtatgggt gcctaaacac agcactctgg ctggaaggcc agggtacagc
4921 gcctactacg gcgaccgcag atcccttttt gtcggcacga aattccatcg tgcagcggtc
4981 gctatgtcgt cgattgaggc ggcttggcct aagaccaagg aagccaatgc taaactcatc
5041 gagtatatac gagggcaaca cctcgttgac gttctaaaga gctgcccggt tgacgacata
5101 cccgtgggca gaccgccttc tagcctaccc tgtggctgca tatacgccat gaccccggaa
5161 cgggtcacag tactgaaaca aagaccgcag gaaggtttcg tggtgtgcag tgcattcaag
5221 ctgcctctca ccaacatcca ggacgtcacc aaggtggagt gcactgtgag agcacccgca
5281 gaggaaccta gaccggtgcg ccatctgcag gagaggcgcc cagcgcaggc cgccgtgagg
5341 cagcttaggc cggcagccgt ggctgccagt gtcgccgcga gtcacacagc cagcaggacc
5401 agcactgcct cgagccgccg cacacctgcg ccgggttcgg tgcaggtgcg tttactgccg
5461 ccgagagatg gtacggaatc acgcagttct cgaatgggct cgcagtccag cgtcacatca
5521 tcagcgggat ccgttccgcc ggcgccccga agggcgccag ctgtgtcggc ggcatcgttg
5581 gcgagcagcg cccatagccg cagtgtgcgc agcgcccctg ctatgagggc cgccagcgcg
5641 ggtgccagaa gcgtgcgcag cgcccaatcc ggttccaccg ggcacagagc gggcgccttc
5701 agcgtcgccg gctcggtgag acagcccagc gggccgccta gtagcgtgag cacacccgcc
5761 gcgatcagag ggctaacacg ggaccagttt gacgccgtaa gagttagggc ccgcaggaac
5821 ttggaacttg aggggtcgga gcacggcagt caatctagct tccactccgg ctcgctggcg
5881 gtggggagct ccgctagtag ctatagccaa cgctccgacg atcaggatac gggcactgaa
5941 ccctcaagcc gcggcgccgc cgtgagaact cggcgcagag ggcaacggga cggcctcgga
6001 gggtacatat tctcatctga tcaaggtacg gctcacctca gccagcacaa cacccagaca
6061 aacaacacaa cggaagtgtt gatgagaacg tcagtactgc cgagcaacga ccacggcaca
6121 ccagatctgc ctgcagaaac gaggaagagg ttggcgtatc aaatgcgccc cactcaaaag
6181 aacaagagtc gctacctgtc cgcgaaggtc cataacatga aacacaagat tgttcgatgt
6241 ttgcagcgag gtgccgggca ctacctgaga gagcagcacg ctctgccgct gtggaaaaac
6301 accttcccta agcccaggta ctccgacgcc tgcgtggtga agttcgagag cgtcaacaca
6361 gcaatcgttg cagccaacat gttcataggg tgtaattacc ccaccttaag ctcgttcggt
6421 atcacggata agtacgatgc ttacctggac atggttgatg ggctcaattg caacctcgac
6481 accgtgacgt tcgaccctgc taaggtgcgt tccctgccga agaaatcaga gtataaccag
6541 ccgctcatac agtcccaagt acccggacct atgacatcca ctctgcaaag catcctcatg
6601 gccgccacca aacgtaactg caacgtcacg cagatgagag agctcccgac tatggactca
6661 gcggcaatga acgtggaggc ttttaaaagt ttcgcctgta aggacaccga cctgtggact
6721 gagttcgcgg aaaaaccagt aaggttgtcg cccggccaaa tcgaagagta tgtctttcat
6781 ctacaagggg ccaaggccaa tgtgatgcac agcagagtcg aagccgtatg ccctgacctc
6841 tcggaggtgg ctatggacag gttcacacta gacatgaaac gcgacgtcaa agtgacgcca
6901 ggcacgaagc acgtagagga gagacctaaa gtccaagaga ttcaagcggc cgaccccatg
6961 gccaccgcgt acttgtgcgc catccataga gagctagtcc gaaggctgaa ggccgtcctg
7021 aaaccgtcta tacacgtgtt gttcgatatg agctccgagg attttgatgc tatcgtgggc
7081 catgggatga agttgggtga caaggtgctg gaaacggaca tctcctcatt cgacaagagc
7141 caggaccaag ccatggcggt tacagcgctg atgctgctga gggacttggg agtagaagaa
7201 gacctcctga ccctaattga ggcgtctttc ggcgacatca cttctgccca cctgcccaca
7261 ggcaccagat ttcagtttgg atcgatgatg aagtctggac tttttctgac gctgttcgtg
7321 aacacgctgc ttaacatcac catagctgcc cgagttttac gggagcagct ggctgatacc
7381 aggtgtgccg cgtttatcgg tgacgacaac gtaatcaccg gagtagtgtc tgacgacatg
7441 atggtggcca ggtgcgcatc ctggctgaac atggaggtga agatcatgga catggaaatt
7501 ggcaacatga gtccttattt ttgtggcggc ttcctgttac tcgacacggt aacaggcact
7561 gtaagccgag tgtcggaccc tgtaaaacgc ctgatgaaga tgggaaaacc ggccctgaac
7621 gatccagaaa cggacgtgga cagatgccgc gcactgcgcg aagaagtgga aagctggtac
7681 agagtgggga ttcagtggcc actgcaggtg gctgccgcca cacgctatgg cgtgaaccac
7741 ctgccgctgg ccacaatggc gatggccacg ctcgcccagg acttgagatc gtacctgggc
7801 gcgcgagggg agtacgtatc cctctacgtc taaccttaat attttctgca tcatacttcc
7861 aaacaatcat gtttcccatg caattcacca actcagccta tcgccagatg gagcccatgt
7921 ttgcaccggg ttcccgagga caagtacagc cgtaccggcc gcgcactaag cgccgccagg
7981 agccgcaagt cggcaacgcc gccattactg ccctcgcgaa ccagatgagt gcgctccagt
8041 tgcaggtagc tggacttgcc ggccaggcaa gggtggaccg ccgtgggcca agacgtgttc
8101 agaagaacaa gcagaagaag aagaactctt ccaacggaga aaaacccaaa gagaagaaga
8161 agaagcaaaa acaacaggag aagaagggaa gcggtggcga aaaagtcaag aagactagga
8221 accgacccgg gaaggaggta aggatctccg taaagtgtgc ccgacagagc accttccccg
8281 tgtaccacga aggtgctata tccggctacg ctgtgctgat tggatctcgc gtattcaagc
8341 cggcacacgt gaagggtaag atcgaccacc ctgaactggc agacatcaag ttccaggtcg
8401 ccgaggacat ggacctcgaa gcagctgcgt acccgaagag catgcgagac caagcggctg
8461 aaccagcgac catgatggac agagtgtaca actgggagta tggcactatc agagtggagg
8521 ataatgtcat aatcgacgca agcggtaggg gcaagccggg tgacagtggc agggccatca
8581 ccgacaactc gggaaaggtt gttggtattg tcctcggagg aggacccgat ggcaggcgca
8641 cacgcctctc cgtgataggt ttcgacaaga agatgaaggc tagggagatc gcctacagtg
8701 atgccatacc ttggacacgc gctccggccc tcctgctgct gcctatggtt attgtctgca
8761 cctacaattc caacaccttc gattgctcca aaccgtcctg ccaggactgc tgcattactg
8821 ctgaaccaga gaaggccatg accatgctga aggacaatct gaacgacccg aactactggg
8881 acctactcat tgctgtcacc acctgtggct ccgcccggag aaagagggct gtgtctacgt
8941 cgcctgccgc cttttacgac acacagatcc tcgccgccca cgcagctgcc tccccataca
9001 gggcgtactg ccccgattgt gacggaacag cgtgtatctc gccgatagcc atcgacgagg
9061 tggtgagcag tggcagcgac cacgtcctcc gcatgcgggt tggttctcaa tcgggagtga
9121 ccgctaaggg tggtgcggcg ggtgaaacct ctctgcgata cctgggaagg gacgggaagg
9181 ttcacgccgc agacaacacg cgactcgtgg tgcgcacgac tgcaaagtgc gacgtgctgc
9241 aggccactgg ccactacatc ctggccaact gcccagtggg gcagagccta accgttgcgg
9301 ccacactgga tggcacccgg catcaatgca ccacggtttt cgaacaccaa gtaacggaga
9361 agttcaccag agaacgcagc aagggccacc atctgtccga catgaccaag aaatgcacca
9421 gattttccac tacaccaaaa aagtccgccc tctacctcgt tgatgtgtat gacgctctgc
9481 cgatttctgt agagattagc accgtcgtaa catgcagcga cagccagtgc acagtgaggg
9541 tgccacctgg taccacagtg aaattcgaca agaaatgcaa gagcgctgac tcggcaaccg
9601 tcactttcac cagcgactcc cagacgttta cgtgtgagga gccagtccta acggctgcca
9661 gtatcaccca gggcaagcca cacctcagat cggcaatgtt gcctagcgga ggcaaggaag
9721 tgaaagcaag gatcccgttc ccgttcccgc cggaaaccgc aacttgcaga gtgagtgtag
9781 ccccactgcc gtcgatcacc tacgaggaaa gcgatgtcct gctagccggt accgcaaaat
9841 accctgtgct gctaaccaca cggaaccttg gtttccatag caacgccaca tccgaatgga
9901 tccagggcaa gtacctgcgc cgcatcccgg tcacgcctca agggatcgag ctaacatggg
9961 gaaacaacgc gccgatgcac ttttggtcat ccgtcaggta cgcatccggg gacgctgatg
10021 cgtacccctg ggaacttctg gtgtaccaca ccaagcacca tccagagtac gcgtgggcgt
10081 ttgtaggagt tgcatgcggc ctgctggcta tcgcagcgtg catgtttgcg tgcgcatgca
10141 gcagggtgcg gtactctctg gtcgccaaca cgttcaactc gaacccacca ccattgaccg
10201 cactgactgc agcactgtgt tgcataccag gggctcgcgc ggaccaaccc tacttggaca
10261 tcattgccta cttgtggacc aacagcaaag tggccttcgg gctacaattt gcggcgcccg
10321 tggcctgtgt gctcatcatt acatacgccc ttaggcactg cagattgtgc tgcaagtctt
10381 ttttaggggt aagagggtgg tcagccctgc tggtcatcct tgcgtatgta cagagctgca
10441 agagctacga acacaccgtg gtggtcccaa tggatccaag agccccgtcg tacgaagcag
10501 tgataaaccg gaatgggtat gatccattga agctgaccat ctcagtgaat ttcaccgtca
10561 tctcaccaac tacggctctg gaatattgga cctgcgcagg agtccccatc gtcgagccgc
10621 cccatgtggg ctgctgcacg tcggtgtcct gcccctctga cctctctacg ctgcatgcgt
10681 ttactggcaa agctgtctcc gacgtgcact gcgatgtgca cacaaacgtg taccccttgt
10741 tgtggggcgc ggctcactgc ttctgttcca ccgagaatac acaggtcagc gctgtggcag
10801 ccaccgtttc tgagttctgt gcccaggact cagagcgtgc cgaagcgttc agcgtacaca
10861 gcagctcagt caccgctgag gtcctggtga cgcttggtga agtggtgacg gcagtccacg
10921 tttacgtgga cggggtaaca tcagccaggg gcactgacct caagatcgtg gctggaccaa
10981 taacaaccga ctactcccca ttcgatcgca aagtagtccg catcggcgaa gaggtctata
11041 actatgactg gcctccttac ggggctggcc gaccaggcac attcggagac attcaagcta
11101 ggtcaaccaa ctatgtcaaa cccaacgatc tgtatgggga catcggaatt gaagtactgc
11161 agccgactaa cgaccacgta catgtggctt acacgtatac gacctctggg ttactgcgtt
11221 ggctgcagga cgctccgaaa ccactcagtg tcacagcacc gcacggttgt aagatcagtg
11281 ccaatccgct cctggccctc gattgtgggg ttggtgccgt ccccatgtcc atcaacattc
11341 cggacgcgaa gtttacccgc aaattaaagg atccgaaacc atcggccctg aaatgcgtgg
11401 tggacagctg cgagtacggg gtggactacg ggggcgccgc cacgatcacc tacgagggcc
11461 acgaggccgg gaagtgcggg attcattccc tgacaccagg agtccccctg agaacatcgg
11521 tggttgaagt ggttgctggc gccaataccg tcaaaacgac cttctcctca cccacgcccg
11581 aggttgcact cgaggtagag atctgttcgg caatagtgaa gtgcgctggt gagtgcactc
11641 caccgaagga acatgtggtc gcaaccaggc ctcgccatgg cagcgaccct ggaggctaca
11701 tctccgggcc cgcaatgcgc tgggccggag ggattgtagg gaccctagtg gtcctgttcc
11761 ttatccttgc cgtcatctac tgcgtggtga agaagtgccg ctccaaaaga atccggatag
11821 tcaagagcta aattccggta tacaaattgc tcactaggag cccatccgat cccacaggga
11881 gtaggatgag tcatctattg gttttaaaat tttcaatac
//
