LOCUS NC_023812 11624 bp ss-RNA linear VRL 13-AUG-2018
DEFINITION Madariaga virus strain MADV/Cebus apella/BRA/BEAN5122/1956,
complete genome.
ACCESSION NC_023812
VERSION NC_023812.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Madariaga virus
ORGANISM Madariaga virus
Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
Martellivirales; Togaviridae; Alphavirus.
REFERENCE 1 (bases 1 to 11624)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2014) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 2 (bases 1 to 11624)
AUTHORS Das,S., Halpin,R.A., Ransier,A., Mohan,M., Fedorova,N., Tsitrin,T.,
Stockwell,T., Amedeo,P., Appalla,L., Bishop,B., Edworthy,P.,
Gupta,N., Hoover,J., Katzel,D., Li,K., Schobel,S., Shrivastava,S.,
Thovarai,V., Wang,S., Auguste,A.J., Wentworth,D.E. and Weaver,S.C.
TITLE Direct Submission
JOURNAL Submitted (14-FEB-2014) J. Craig Venter Institute, 9704 Medical
Center Drive, Rockville, MD 20850, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to KJ469640.
This work was supported by the National Institute of Allergy and
Infectious Diseases (NIAID), Genome Sequencing Centers for
Infectious Diseases (GSCID) program.
##Genome-Assembly-Data-START##
Current Finishing Status :: Finished
Assembly Method :: clc_ref_assemble_long v. 3.22.55705
Genome Coverage :: 189.2x
Sequencing Technology :: Illumina
##Genome-Assembly-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..11624
/organism="Madariaga virus"
/mol_type="genomic RNA"
/strain="MADV/Cebus apella/BRA/BEAN5122/1956"
/host="Cebus apella"
/db_xref="taxon:1440170"
/country="Brazil"
/collection_date="1956"
5'UTR 1..42
gene 43..7467
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/db_xref="GeneID:18748673"
CDS 43..7467
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/codon_start=1
/transl_except=(pos:5620..5622,aa:Arg)
/product="non-structural polyprotein precursor P1234"
/protein_id="YP_009020570.1"
/db_xref="GeneID:18748673"
/translation="MEKVHVDLDADSPYVKSLQKCFPHFEIEATQVTDNDHANARAFS
HLATKLIESEVDQDQVILDIGSAPVRHTHSKHKYHCICPMISAEDPDRLHRYADKLRK
SDVTDRFIASKAADLLTVMSTPDVETPSLCMHTDSTCRYHGTVAVYQDVYAVHAPTSI
YHQALKGVRTIYWIGFDTTPFMYKNMAGAYPTYNTNWADESVLEARNIGLCGSDLHEK
RLGKISIMRKKKLQPTNKVVFSVGSTIYTEERILLRSWHLPNVFHLKGKTSFTGRCNT
IVSCEGYVVKKITISPGIYGKVDNLASTLHREGFLSCKVTDTLRGERVSFPVCTYVPA
TLCDQMTGILATDVSVDDAQKLLVGLNQRIVVNGRTQRNTNTMQNYLLPVVAQAFSRW
AREYRADLEDEKDLGVRERSLVMGCCWAFKTHKITSIYKKPGTQTIKKVPAVFNSFVI
PQFNSYGLDIGLRRRIKMLLEEKRKPAPIITEADVAHLKGMQEEAEAVAEAEAVRAAL
PPLLPEVERETIEADIDLIMQEAGAGSVETPRRHIKVTTYPGEETIGSYAVLSPQAVL
NSEKLACIHPLAEQVLVMTHKGRAGRYKVEPYHGRVVVPSGTAIPIPDFQALSESATI
VYNEREFVNRYLHHIAINGGAINTDEEYYKVLRSSEADSEYVFDIDARKCVKKADAGP
MCLVGELVDPPFHEFAYESLKTRPAAPHKVPTIGVYGVPGSGKSGIIKSAVTKRDLVV
SAKKENCTEIIKDVKRMRGMDIAARTVDSVLLNGVKHPVDTLYIDEAFACHAGTLLAL
IAIVKPKKVVLCGDPKQCGFFNMMCLKVHFNHEICTEVYHKSISRRCTKTVTAIVSTL
FYDKRMRTVNPCSDKIIIDTTSTTKPQRDDIILTCFRGWVKQLQIDYKNHEIMTAAAS
QGLTRKGVYAVRYKVNENPLYAQTSEHVNVLLTRTEKRIVWKTLAGDPWIKTLTAHYP
GEFSATLEEWQAEHDAIMERILETPASSDVYQNKVHVCWAKALEPVLATANITLTRSQ
WETIPAFKDDKAFSPEMALNFLCTRFFGVDIDSGLFSAPTVPLTYTNEHWDNSPGPNR
YGLCMRTAKELARRYPCILKAVDTGRLADVRTNTIKDYSPLINVVPLNRRLPHSLVVS
HRYTGDGNYSQLLSKLIGKTVLVIGTPISVPGKRVETLGPGPQCTYKADLDLGIPSTI
GKYDIIFVNVRTPYKHHHYQQCEDHAIHHSMLTRKAVDHLNKGGTCVALGYGTADRAT
ENIISAVARSFRFSRVCQPKCAWENTEVAFVFFGKDNGNHLRDQDQLSIVLNNIYQGS
TQYEAGRAPAYRVIRGDISKSTDEAIVNAANNKGQPGAGVCGALYKKWPGAFDKVPIA
TGTAHLVKHTPNIIHAVGPNFSRVSEVEGNQKLSEVYMDIAKIINRERYNKVSIPLLS
TGIYAGGKDRVMQSLNHLFTAMDTTDADVTIYCLDKQWEARIKDAIVRKESVEELVED
DKPVDIELVRVHPLSSLVGRPGYSTDEGKVHSYLEGTRFHQTAKDIAEIYAMWPNKQE
ANEQICLYVLGESMTSIRSKCPVEDSEASSPPHTIPCLCNYAMTAERVYRLRMAKNEQ
FAVCSSFQLPKYRITGVQKIQCNKPVIFSGVVPPAIHPRKFSAIEETVPVTIERLVPR
RPAPPVPVPARIPSPRCSPAVSMQSLGGSSTSDVVISEAEVHDSDSECSVPPMPFVVE
AEVHASQGSHWSIPSASGFEIRELPEDRSIPGSPTRTSVISDHSVNLITFDSVTDIFE
NFKQAPFQFLSEIRPIPAPRRRVGGLETDTKRYDKTEEKPIPKPRTRTMKYKQPPGVA
RSISEAELDEFIRRHSNRRYEAGAYIFSSETGQGHLQQKSTRQCKLQNPILERSVHEK
FYAPRLDLEREKLLQKKLQLCASEGNRSRYQSRKVENMKAITAERLLQGIGAYLSAES
QPVECYKVNYPVPIYSTTRSNRFSSADVAVRVCNLVLQENFPTVASYTITDEYNAYLD
MVDGASCCLDTATFCPAKLRSFPKKHSYLRPEIRSAVPSPIQNTLQNVLAAATKRNCN
VTQMRELPVLDSAAFNVECFKKYACNDEYWDTFKNNPIRLTTENVTQYVTKLKGPKAA
ALFAKTHNLQPLHEIPMDRFVMDLKRDVKVTPGTKHTEERPKVQVIQAADPLATAYLC
GIHRELVRRLNAVLLPNIHTLFDMSAEDFDAIIAEHFQFGDAVLETDIASFDKSEDDA
IAMSALMILEDLGVDQALLDLIEAAFGNITSVHLPTGTRFKFGAMMKSGMFLTLFINT
VVNIMIASRVLRERLTNSPCAAFIGDDNIVKGVKSDALMAERCATWLNMEVKIIDAIV
GVKAPYFCGGFIVVDQVTGTACRVADPLKRLFKLGKPLPLDDDQDGDRRRALYDEALR
WNRIGITDELIKAVESRYEVFYTSLVITALTTLAATVSNFKHIRGNPITLYG"
mat_peptide 43..1641
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/product="mRNA-capping enzyme nsp1"
/protein_id="YP_009020583.1"
misc_feature 88..1158
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="Viral methyltransferase; Region: Vmethyltransf;
pfam01660"
/db_xref="CDD:396298"
mat_peptide 1642..4023
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/product="protease nsp2"
/protein_id="YP_009020584.1"
misc_feature 2191..2904
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="Viral (Superfamily 1) RNA helicase; Region:
Viral_helicase1; pfam01443"
/db_xref="CDD:366646"
mat_peptide 4024..5640
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/product="non-structural protein nsp3"
/protein_id="YP_009020585.1"
misc_feature 4069..4449
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="X-domain (or Mac1 domain) of viral non-structural
protein 3 and related macrodomains; Region:
Macro_X_Nsp3-like; cd21557"
/db_xref="CDD:438957"
misc_feature order(4087..4095,4105..4125,4339..4344,4348..4362,
4444..4446)
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="ADP-ribose binding site [chemical binding]; other
site"
/db_xref="CDD:438957"
mat_peptide 5641..7464
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/product="RNA-directed RNA polymerase nsp4"
/protein_id="YP_009020586.1"
misc_feature 6091..7464
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="RNA-dependent RNA polymerase (RdRp) in the family
Togaviridae of positive-sense single-stranded RNA
[(+)ssRNA] viruses; Region: Togaviridae_RdRp; cd23250"
/db_xref="CDD:438100"
misc_feature 6736..6780
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438100"
misc_feature order(6751..6753,7033..7041)
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="catalytic residues [active]"
/db_xref="CDD:438100"
misc_feature 6922..6993
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438100"
misc_feature 7015..7059
/gene="NSP"
/locus_tag="F782_44812gpNSP"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438100"
gene 7541..11269
/gene="SP"
/locus_tag="F782_44812gpSP"
/db_xref="GeneID:18748672"
CDS 7541..11269
/gene="SP"
/locus_tag="F782_44812gpSP"
/codon_start=1
/product="structural polyprotein"
/protein_id="YP_009020571.1"
/db_xref="GeneID:18748672"
/translation="MFPYPTLNYPPMAPVNPMAYRDPNPPRRRWRPFRPPLAAQIEDL
RRSIANLTFKQRAPNPPAGPPAKRKKPAPKPKPAAPKKKRQPPPAKKQKRKQKPGKRQ
RMCMKLESDKTFPIMLKGQVNGYACVVGGRVFKPLHVEGKIDNEQLAAIKLKKASIYD
LEYGDVPQCMKSDTLQYTSEKPPGFYNWHHGAVQYENNRFTVPRGVGGKGDSGRPILD
NRGRVVAIVLGGANEGSRTALSVVTWNQKGVTVKDTPEGSEPWSLTTVMCVLANITFP
CDQPPCMPCCYEKNPHETLSMLEQNYDSQAYDLLLDAAVKCNGRRTRRDLETHFTQYK
LARPYIADCSNCGHGRCDSPIAIEDIRGDAHAGYIRIQTSAMFGLKSDGVDLAYMSFM
NGKTLKAIKIEHLYARTSAPCSLVSYHGYYILAQCPPGDTVTVGFQDGANKHMCTIAH
KVEFKPVGREKYRHPPEHGVELPCTKYTHKRADQGHYVEMHQPGLVADHSLLSMSSTK
VKITVPSGSQVKYYCKCPDVKEGTTGSDYTTACTNLKQCRAYLIDNKKWVYNSGKLPR
GEGETFKGKLHVPFVPVTSKCTATLAPEPLVEHKHRSLILHLHPEHPTLLTTRALGSN
ARPTRQWIEQPTTVNFTVTGEGFEYTWGNHPPKRVWAQESGEGNPHGWPHEIVIYYYN
RYPMTTVIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYRLAPNAQVPILLAVLCCVK
PTRADDTLQVLNYLWNNNQNFFWMQTLIPLAALIVCMRMLRCLLCCGPAFLLVCGALG
AAAYEHTAVMPNKVGIPYKALVERPGYAPVHLQIQLVTTKIIPSANLEYITCKYKTKV
PSPVVKCCGSTQCSAKSHPDYQCQVFTGVYPFMWGGAYCFCDTENTQMSEVYIERAEE
CSVDQAKAYKVHTGTVQAVVNITYGSVSWRSADVYVNGETPAKIGDAKLTIGPLSSAW
SPFDSKVVVYGHEVYNYDFPEYGTGKAGSFGDLQSRTPTSNDLYANTNLKLQRPQPGV
VHTPYTQAPSGFERWKKDRGAPLNDIAPFGCTIALDPLRAENCAVGNIPLSIDIPDAA
FTRIAETPTVSDLECKVTECTYASDFGGIATISYKASKSGNCPIHSPSGIAVIKENDV
TLADSGAFTFHFSTASIHPAFKMQVCTSVVTCKGDCKPPKDHIVDYPAQHTETYTSAV
SATAWSWLKVLVGSTSAFIVLGLIATAVVALVLFTHRH"
mat_peptide 7541..8323
/gene="SP"
/locus_tag="F782_44812gpSP"
/product="capsid protein"
/protein_id="YP_009020587.1"
misc_feature 7853..8323
/gene="SP"
/locus_tag="F782_44812gpSP"
/note="Alphavirus core protein; Region: Peptidase_S3;
pfam00944"
/db_xref="CDD:366379"
mat_peptide 8324..8512
/gene="SP"
/locus_tag="F782_44812gpSP"
/product="E3 protein"
/protein_id="YP_009020588.1"
misc_feature 8339..8512
/gene="SP"
/locus_tag="F782_44812gpSP"
/note="Alphavirus E3 glycoprotein; Region:
Alpha_E3_glycop; pfam01563"
/db_xref="CDD:396236"
mat_peptide 8513..9772
/gene="SP"
/locus_tag="F782_44812gpSP"
/product="E2 envelope glycoprotein"
/protein_id="YP_009020589.1"
misc_feature 8552..9754
/gene="SP"
/locus_tag="F782_44812gpSP"
/note="Alphavirus E2 glycoprotein; Region:
Alpha_E2_glycop; pfam00943"
/db_xref="CDD:425959"
mat_peptide 9773..9943
/gene="SP"
/locus_tag="F782_44812gpSP"
/product="6K membrane protein"
/protein_id="YP_009020590.1"
misc_feature 9797..11257
/gene="SP"
/locus_tag="F782_44812gpSP"
/note="Alphavirus E1 glycoprotein; Region:
Alpha_E1_glycop; pfam01589"
/db_xref="CDD:279870"
mat_peptide 9944..11266
/gene="SP"
/locus_tag="F782_44812gpSP"
/product="E1 envelope glycoprotein"
/protein_id="YP_009020591.1"
3'UTR 11270..11602
ORIGIN
1 gggtatggtg tagaggcaac cacccgacct atcctatcca aaatggagaa agtacacgtg
61 gacttagacg ctgacagccc ttacgtcaag tcactgcaga agtgctttcc gcattttgag
121 atagaagcca cgcaggtcac tgacaatgac catgctaatg ctagagcgtt ttcgcatcta
181 gccaccaaac tcatcgaaag cgaagtggac caagaccagg ttatcctgga tattggaagc
241 gcgcctgtgc ggcacacgca ttccaagcac aagtaccact gtatctgccc gatgatcagt
301 gcggaagacc ctgatcgact ccataggtat gcagataagc tgagaaagag tgacgtaaca
361 gataggttta tagcctctaa agctgctgac ctactgacgg tgatgtcaac accagacgta
421 gagactccat cactatgcat gcacacagat tccacttgca ggtaccacgg tactgttgcc
481 gtataccaag atgtatacgc agtgcatgca cctacctcta tttaccacca agcactgaaa
541 ggtgtgcgca ccatctactg gatagggttt gataccaccc cattcatgta caagaatatg
601 gcaggtgcgt accctactta taacactaat tgggccgatg aaagtgtgct agaggcaagg
661 aacattggtc tgtgcggctc agacctgcat gaaaaacgac ttggaaagat ctccatcatg
721 agaaaaaaga aattgcaacc tactaacaaa gttgtgtttt ctgtaggttc aacaatatac
781 actgaagaaa gaatactttt gcgcagctgg cacctgccga acgttttcca tcttaaaggg
841 aagacgagtt ttacaggcag atgcaatact atcgtcagct gcgagggtta cgttgtaaag
901 aagattacca tcagtcccgg tatctacggg aaggttgata atctggcttc taccttgcac
961 cgcgagggtt tcttgagctg caaggtgaca gatacgttgc gaggggagag ggtatccttt
1021 cccgtgtgca cgtatgtacc ggcaacactg tgcgatcaga tgacagggat tctggctact
1081 gatgtcagtg tcgacgacgc tcagaaactg ctggttgggc tcaaccagcg catcgtcgtc
1141 aatgggagaa cacaacgtaa cacaaatact atgcagaatt accttttgcc cgtagtagcc
1201 caagcgttct ccaggtgggc gcgtgaatat cgcgctgact tggaggacga aaaagaccta
1261 ggggtgcggg aacgctccct agtcatgggc tgctgctggg ccttcaagac tcataaaatc
1321 acatccattt acaagaaacc tggtacccaa actatcaaga aggtaccagc ggtctttaac
1381 tccttcgtca tcccccaatt caacagctac ggattggata tagggttacg ccgccgaatt
1441 aaaatgctcc tagaagagaa gaggaagccg gcccctatta tcaccgaagc cgacgttgcc
1501 catcttaagg ggatgcaaga agaggcagag gcggtagcag aagctgaagc tgtgagagca
1561 gctttaccgc cacttttgcc tgaagtagag agagagacca ttgaagcaga catagatctg
1621 ataatgcaag aagcaggtgc gggaagtgtc gagacgccca gacggcacat aaaagtcaca
1681 acatatccag gtgaagagac gattggatct tatgcagtgc tctcgccaca ggctgtcctt
1741 aacagcgaga aattagcctg cattcacccg ctggctgaac aggtgctcgt catgacccat
1801 aaagggcgtg caggtaggta taaagtcgaa ccctaccatg gacgggtagt tgtccctagt
1861 ggtactgcca ttccaattcc agatttccaa gcgctgagcg agagtgcaac catcgtctac
1921 aatgaacggg aatttgtcaa tcggtactta caccatattg ccatcaatgg aggtgcgata
1981 aacaccgacg aagagtacta caaagtcctt agaagtagcg aggcggattc ggagtacgtc
2041 ttcgatattg acgccagaaa gtgcgtcaaa aaggctgacg caggaccgat gtgccttgtt
2101 ggtgagctgg ttgacccacc ctttcacgag tttgcatacg aaagtctgaa aacccgtcca
2161 gcagcgcccc ataaggtgcc gactattgga gtctacggag ttcctggctc aggaaaatcg
2221 ggaatcatca agagcgcagt cacgaaaaga gacctagtgg tcagcgcaaa gaaggagaac
2281 tgcacggaaa ttatcaaaga tgttaaacgt atgcgaggca tggatattgc agcccgtacg
2341 gtggactccg tgttgcttaa cggggtcaaa catccagtag acacactgta cattgacgag
2401 gcctttgcat gccacgcggg gacgttacta gcactcattg ccattgtcaa accaaagaaa
2461 gtagtattgt gtggagaccc gaaacagtgt ggattcttta acatgatgtg tctaaaagtg
2521 catttcaacc atgaaatatg caccgaagtg taccacaaga gtatctcccg caggtgcacc
2581 aagacagtca cagctatcgt ctctacatta ttctatgaca agagaatgag aacggtgaat
2641 ccgtgcagcg acaagatcat tatagacacc accagcacaa caaaaccaca acgagatgac
2701 attatactga catgctttag agggtgggtt aagcaactac aaattgacta taaaaaccac
2761 gagatcatga ccgcggccgc atctcaagga ttaacccgca aaggtgtgta cgcagtcaga
2821 tacaaagtca atgaaaatcc cctctatgca cagacatctg aacacgtaaa tgtcctcttg
2881 acccgcaccg agaaacgcat tgtttggaaa actcttgccg gggacccttg gataaagacc
2941 ctaacggctc actaccctgg ggaattttcg gcaacattgg aggagtggca agccgagcat
3001 gatgctatta tggagcgtat cctggaaact ccagctagta gcgatgtata ccagaataaa
3061 gtgcacgttt gctgggcaaa agcacttgaa ccagtcttgg ccactgccaa tataacactc
3121 actcgttccc aatgggagac catccctgcc ttcaaagacg acaaggcttt ctccccggaa
3181 atggcgttga acttcttgtg caccaggttc ttcggggttg acattgacag tggattattc
3241 tcagcgccaa ctgtaccttt aacatacaca aatgaacatt gggataacag ccccggcccg
3301 aacagatacg gcttgtgtat gcgtactgcc aaagaactgg ctcgtcgcta tccatgcata
3361 ctgaaagctg tagacacagg ccgacttgcg gatgtgcgta ccaataccat caaagactat
3421 agccccctga tcaacgtggt gccactcaac cgtcgccttc cacactcatt ggtcgtgtcg
3481 cacagatata ccggtgacgg aaattattct cagctgctgt caaagttgat aggaaaaacc
3541 gtgctggtaa taggaacccc aatttccgtt cctggaaaac gtgtagagac gctaggccct
3601 ggtccacagt gcacgtacaa agcagacttg gacttaggca tccccagcac gattggaaaa
3661 tatgacataa tatttgtcaa cgtcaggaca ccgtacaagc accaccatta tcagcagtgt
3721 gaagaccacg ccattcacca cagcatgctt actagaaagg ccgttgatca cctgaacaag
3781 ggaggcactt gcgtggcact aggctacggc accgcagata gagcgaccga aaatattatt
3841 tcagcggtag ctcgatcttt tagattttcg agagtctgtc agcctaagtg tgcgtgggaa
3901 aacactgagg tcgcgtttgt cttttttggc aaggacaatg gtaatcacct acgcgaccag
3961 gatcaactaa gtattgtact gaataacatc taccaaggtt ctacccagta cgaagcgggc
4021 agagcaccag cttacagagt aattcgggga gacatatcta agagcaccga cgaagccatt
4081 gttaatgcag ctaacaataa aggacaaccg ggagccggag tatgtggagc attgtataag
4141 aaatggccag gggcttttga taaggtgccc attgcgacag gtactgcgca cctagttaag
4201 catacgccca acattatcca tgcagtcggg ccgaattttt cccgcgtatc cgaagtggaa
4261 ggcaaccaga agctatcgga ggtctacatg gacattgcaa aaatcatcaa cagggaacga
4321 tacaataagg tgtcaatacc tctgttatca actggtattt acgcaggtgg caaggatcgc
4381 gtgatgcaat cgttaaacca cctgtttact gccatggaca ccaccgatgc agatgtaact
4441 atctattgcc tggacaaaca gtgggaagca cgaatcaaag acgccatcgt ccggaaggaa
4501 agcgtcgaag aactcgtaga agatgacaaa cctgtcgata tcgagttggt ccgtgtgcac
4561 ccactaagta gtctggtcgg gagaccaggt tactctacgg acgaaggcaa ggtgcactct
4621 tatctggaag gaacaaggtt ccaccaaact gccaaagaca tcgcagagat ttacgccatg
4681 tggcctaata aacaagaagc taatgagcaa atctgtctgt acgtactagg agaaagcatg
4741 accagcattc gctctaagtg ccctgtagaa gattccgaag cctcatctcc accgcacacg
4801 attccgtgtc tttgcaatta tgccatgacg gccgagcggg tctacagatt acgcatggca
4861 aagaatgagc agtttgccgt gtgttcatca tttcaattac ccaagtacag aataacgggc
4921 gtgcagaaga tccagtgcaa caagccagtc atattctcgg gggttgtacc accagcaata
4981 catccacgga aattctcggc tattgaggaa acagtgccag taacaattga acggcttgtt
5041 ccaagacgtc ctgctccacc ggtgccggta cctgcccgta ttcctagccc acgctgctca
5101 ccagcagtta gcatgcagtc tttgggcggt agtagtacat cggacgttgt tatctctgaa
5161 gccgaagttc acgattcaga ctccgaatgc agtgtccctc caatgccttt cgtcgtggag
5221 gcagaggttc acgcgagtca aggctcacac tggagcattc ctagcgcgtc tggcttcgaa
5281 atccgtgaac tgccagaaga tcgcagcata cccggctcac cgacacgcac gtcggtaatt
5341 tctgaccact ccgtgaatct gatcacattt gacagcgtta ctgacatatt tgaaaacttc
5401 aaacaagcgc cttttcagtt tctatcagag atccgcccaa taccagcacc gcgtcgcaga
5461 gtgggagggc ttgagactga tactaagcgc tacgacaaga cagaggaaaa accaattcct
5521 aagccacgta cgcgcacaat gaagtacaag cagcctccag gtgtagccag gtccatttcg
5581 gaagcagaat tggatgagtt tatccgccga cattcaaatt gacggtatga agcgggtgcg
5641 tatattttct cgtcagagac agggcaaggg catctgcaac agaaatcaac tcggcagtgc
5701 aaactccaaa atcctatctt ggagcgctct gtccacgaga aattctacgc cccgcgcctc
5761 gacttggaac gtgagaaact gctacagaaa aaactacaat tgtgcgcgtc ggaaggtaat
5821 aggagtaggt accaatcacg taaagtcgag aatatgaaag caattacggc ggaacgtctg
5881 ttgcaaggga taggggctta tctttcagct gaatcgcagc ctgtggagtg ctataaagtt
5941 aactaccccg tccccattta ctccaccaca cgcagcaaca gattctcatc ggcagacgtc
6001 gcggtaaggg tatgtaattt ggtattgcaa gagaactttc ctactgtggc gagctacacc
6061 atcaccgatg agtacaacgc ctatttagat atggttgacg gagcatcgtg ctgcttagat
6121 actgcgacct tctgcccggc taagctacgc agtttcccga aaaagcatag ctacttgcgg
6181 cctgagataa gatcagctgt tccgtccccc atacagaaca cacttcagaa cgttttagcg
6241 gcagccacca aacgaaactg taatgtcacc cagatgcgcg agttaccggt cctggactcg
6301 gccgcattta acgtggagtg ctttaaaaaa tatgcatgta acgacgaata ctgggataca
6361 tttaaaaaca accccattag gctcactact gagaatgtca ctcagtatgt tactaaactc
6421 aaaggaccaa aagctgctgc actgttcgct aagacgcata acttacaacc actgcatgaa
6481 attccgatgg atagatttgt gatggatctt aagcgcgacg tcaaagtaac tccagggacc
6541 aagcataccg aagaacgacc gaaagtccaa gtgatacagg cggcggaccc cctggcgacc
6601 gcatacttgt gcggaataca tagggagttg gtaagaagac taaacgccgt actgctgcct
6661 aacattcaca cgttgttcga tatgtccgct gaagactttg atgccatcat agccgaacat
6721 ttccaattcg gagacgcagt attggaaaca gatatcgctt cgttcgacaa aagtgaggat
6781 gatgccattg ctatgtctgc acttatgatc cttgaagacc tgggtgtcga tcaggccctc
6841 ctagatctta tagaagctgc ttttgggaat atcacatctg tccacctccc aacagggaca
6901 cgcttcaagt tcggagctat gatgaagtca ggtatgtttt taaccctgtt tattaatact
6961 gtggttaaca ttatgattgc cagccgcgtg ttgcgtgaga ggctaactaa ttcgccttgc
7021 gctgctttta ttggcgatga taatattgtg aaaggagtca agtctgacgc actcatggcc
7081 gaacgttgtg ccacttggtt gaacatggaa gtaaaaataa tagatgcaat cgtgggtgta
7141 aaagctccgt atttctgcgg aggctttatc gtcgtggacc aagtgactgg tacagcatgc
7201 agagtggcag acccccttaa gaggctgttt aaacttggta agcctttgcc cctggacgac
7261 gaccaggatg gggataggcg cagagcattg tacgacgaag cgctcagatg gaaccggatc
7321 ggtatcactg atgagctaat caaagccgtt gaatcacgat atgaagtgtt ttacacctca
7381 ttggttatca ccgctttgac tactcttgca gctacagtca gcaatttcaa acacataaga
7441 ggaaacccta taaccctcta cggctgacct aaataggttg tgcagaagaa gctaacctat
7501 tatacaacac tcagtgtatc acaagcgcaa taccgaaacg atgtttccgt atccaacatt
7561 gaactacccg cctatggcac cggttaatcc gatggcatac agggacccca atccaccaag
7621 acgtaggtgg cggccatttc ggccgcccct agctgctcaa atcgaggatt tgagacgttc
7681 catcgctaac ctaacgttta agcaaagggc cccgaacccg ccagcaggcc ccccggcaaa
7741 acgtaagaag ccagcaccta aaccgaagcc agcggcgccc aagaaaaaga ggcaaccacc
7801 tccagctaag aagcagaagc gcaaacaaaa gcctggtaaa cggcaaagga tgtgtatgaa
7861 actggaatcc gacaagactt tccccatcat gttgaaaggc caagtgaacg gctatgcgtg
7921 cgtagtcggc gggcgcgtgt tcaaaccgct gcatgttgaa ggcaagattg acaacgaaca
7981 gttggccgcc attaaactga agaaagcaag tatctatgat ctcgagtatg gcgatgttcc
8041 acaatgtatg aaatcggaca ccctccagta caccagtgag aagccgccgg gtttctacaa
8101 ttggcaccac ggagcagtgc agtatgaaaa caacaggttt accgtaccac gaggagtggg
8161 cggaaaaggg gatagcggac gcccgatttt agacaatcgc ggccgtgtgg tcgctatagt
8221 actgggcgga gctaacgaag gatctcgcac agcgctgtca gtggtcacgt ggaaccagaa
8281 aggggttacc gtgaaggata cccccgaagg ttccgaacct tggtcactga ctacagttat
8341 gtgcgtctta gctaacatca ccttcccatg tgaccagcca ccctgtatgc cctgctgtta
8401 tgagaaaaat ccgcacgaga cgctcagcat gctcgaacag aactatgaca gccaggctta
8461 tgacctactg ctggacgcgg ccgtcaaatg taacggcagg agaacccgta gagacctgga
8521 aactcatttc acacagtaca agctagctcg cccatacata gcagactgct ccaattgcgg
8581 ccacggcaga tgcgatagtc ccattgccat tgaggacatt cgcggagacg ctcatgcagg
8641 ctacatccga atacaaacat cggcaatgtt cggcctgaag tcggatggag tagatctagc
8701 ctatatgagc ttcatgaacg gcaaaaccct gaaggccata aaaatagaac atttgtatgc
8761 tcgtacatca gcaccatgct ctctggtttc ctaccatggt tactacatcc ttgcccagtg
8821 tcctcccgga gacactgtga cagtggggtt tcaagacggt gccaacaagc acatgtgcac
8881 cattgcccat aaagttgagt tcaaacctgt aggaagagag aagtaccgcc atccacctga
8941 acacggcgtg gagttaccct gcaccaagta cacgcacaaa cgtgcagacc agggccacta
9001 cgtcgaaatg caccaaccag ggctggtagc cgaccactcg ctactgagca tgagcagcac
9061 gaaagtgaaa atcaccgtcc caagtggttc tcaagtgaag tactactgta agtgccccga
9121 cgtaaaggag ggaacaaccg gcagcgacta cacgactgct tgcacgaacc tgaagcagtg
9181 tagagcgtat cttattgaca ataagaaatg ggtctacaac tctggcaagt tacctagagg
9241 agaaggcgaa acctttaaag gtaaactcca tgtgccgttc gtcccggtca cgagcaagtg
9301 taccgcgacc ttggcccccg aaccactggt ggaacacaag catcgatccc taatcctaca
9361 cctgcaccca gaacacccaa cactactgac gaccagagca ctgggaagta acgcgaggcc
9421 gacaaggcag tggatcgaac aaccaaccac agtcaatttt acagtaacag gagaaggttt
9481 tgaatatacc tggggcaacc acccgcccaa acgagtttgg gcccaagaat caggtgaagg
9541 aaatcctcat ggttggccgc acgaaatagt aatttattac tacaaccggt atccaatgac
9601 aactgtcatt ggactgtgca cgtgcgtagc catcatcatg gtttcctgtg ttacatctgt
9661 atggcttctt tgccgcactc gcaatctttg cataacacca tacagattag cacctaacgc
9721 ccaggtaccc atcttattag cagtcctatg ctgcgtgaaa ccaaccagag cagatgacac
9781 tttgcaggtc ctaaattatc tgtggaataa caaccagaac ttcttttgga tgcagaccct
9841 tataccactg gcagctttga tagtgtgcat gcgcatgttg cgctgcttac tatgctgcgg
9901 tccggctttt ttacttgtct gcggcgcctt gggcgccgca gcatacgaac acacagcagt
9961 gatgccgaac aaggtgggga tcccctacaa agcgctggtt gaacgcccgg gctatgcacc
10021 agtccacctg caaattcagt tggtaactac taaaatcatt ccgtcagcga atctggaata
10081 tatcacctgc aagtataaaa ccaaggtgcc ctctcctgta gtcaaatgct gcggctccac
10141 ccagtgctca gcaaaatcac accctgacta ccaatgtcaa gtgttcacag gggtctaccc
10201 atttatgtgg ggaggagcct attgcttctg cgataccgag aatacgcaga tgagcgaagt
10261 gtacattgaa cgcgccgagg aatgttcagt cgaccaggct aaggcataca aggtgcacac
10321 tggcacagtg caggcggtgg tcaatatcac ctacgggagc gtcagctggc gatcggctga
10381 cgtttacgtc aacggtgaaa cacctgcaaa gatcggcgat gccaaattaa ctataggacc
10441 tttgtcctcc gcctggtctc cattcgactc aaaggtcgta gtgtatgggc acgaggtgta
10501 caattatgac tttccagaat acggcacagg aaaagccggt tcctttggtg atctgcaatc
10561 cagaacaccc accagtaacg acttgtacgc caatacaaac ctgaagttgc aacgaccaca
10621 accaggagta gtccatacgc cttataccca ggcgccatcc gggttcgaac gctggaagaa
10681 agaccgagga gctccattga atgacatcgc tccctttggg tgcacgatag ctttagaccc
10741 actgcgagcg gagaactgcg ctgtgggcaa catccccctt tctatagaca tcccggatgc
10801 cgcatttacc aggatcgcag agacgcccac tgtgtccgac ctggaatgca aggttactga
10861 atgcacatac gcatccgact tcgggggaat tgccaccatc agctataagg caagcaaatc
10921 aggtaactgc cccattcact ccccgtcagg cattgcagta atcaaagaaa acgatgttac
10981 actggcagac agcggtgcct tcacgtttca cttttctaca gccagcatcc acccggcatt
11041 caaaatgcag gtgtgtacta gcgtagttac ctgcaaaggt gactgtaagc caccaaagga
11101 ccacattgtc gattacccgg ctcaacacac agaaacgtac acatcagcag tatcggcaac
11161 tgcttggtca tggctgaaag tgctagtggg aagcacatca gcatttattg tgctggggtt
11221 gattgccact gcagtggtcg ccctggtact attcacccac agacactaac gtactatcat
11281 tattatatat catcatggtt cgacgtactt ccgagccacg atgacggtgg tgcataatgc
11341 cacctgcgca gtgcataatg ctgcgactta taggtagtac gctacccttt ataacactac
11401 tggcagtgaa taatgctgcc ttttataaaa acactactgg cggcgcataa tgctgccttt
11461 tataacacta ctggcagtta caacgctgcc ttttataaac ttttacaaca ctactggcgg
11521 cgcataatgc tgccttttat aaaatcttta aaattcatat acaatttttt cttttatgtt
11581 tttattttgt ttttaatatt tcaaaaaaaa aaaaaaaaaa aaaa
//
