LOCUS NC_025363 15312 bp RNA linear VRL 13-AUG-2018
DEFINITION Avian paramyxovirus 12 isolate Wigeon/Italy/3920_1/2005, complete
genome.
ACCESSION NC_025363
VERSION NC_025363.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Avian orthoavulavirus 12
ORGANISM Avian orthoavulavirus 12
Viruses; Riboviria; Orthornavirae; Negarnaviricota;
Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae;
Avulavirinae; Orthoavulavirus; Orthoavulavirus italiense.
REFERENCE 1 (bases 1 to 15312)
AUTHORS Terregino,C., Aldous,E., Heidari,A., Fuller,C.M., De Nardi,R.,
Manvell,R.J., Beato,M.S., Shell,W.M., Monne,I., brown,I.H.,
Alexander,D.J. and Capua,I.
TITLE Antigenic and genetic analyses of isolate
APMV/wigeon/Italy/3920-1/2005 indicate that it represents a new
Avian paramyxovirus (APMV-12)
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 15312)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (26-OCT-2014) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 15312)
AUTHORS Terregino,C., Aldous,E., Heidari,A., Fuller,C.M., De Nardi,R.,
Manvell,R.J., Beato,M.S., Shell,W.M., Monne,I., brown,I.H.,
Alexander,D.J. and Capua,I.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2012) OIE/FAO Reference Laboratory for Avian
Influenza and Newcastle Disease, Istituto Zooprofilattico
Sperimentale delle Venezie, Viale dell' Universita 10, Legnaro,
Padua 35020, Italy
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to KC333050.
##Assembly-Data-START##
Assembly Method :: Seqscape v. v2.6
Sequencing Technology :: Sanger dideoxy sequencing; 454
##Assembly-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..15312
/organism="Avian orthoavulavirus 12"
/mol_type="genomic RNA"
/isolate="Wigeon/Italy/3920_1/2005"
/host="wigeon"
/db_xref="taxon:2560320"
/country="Italy"
/collection_date="01-Nov-2005"
gene 122..1603
/gene="NP"
/locus_tag="NZ93_gp1"
/db_xref="GeneID:20964452"
CDS 122..1603
/gene="NP"
/locus_tag="NZ93_gp1"
/codon_start=1
/product="nucleoprotein"
/protein_id="YP_009094168.1"
/db_xref="GeneID:20964452"
/translation="MASVFEEYDKLLESQTRPSKGVSPAEKGGTLKVELPVFVCNSND
AETRWAFVCFALRLAVSDSSNKPLRQGAMISLLCAHSETMRTHVAMAGRNGETSIALL
EIDAFVDGMPVFNSRSGITDEKAQRFSMIAGDLPRSCSNNTPFINADAENDPPEDITD
ALERILTVQTQLWVTLAKAMTSYETAEESEVRRITKYTQQGRVIKKFLLFPVVRSSIQ
VTIRSSLAVRAFMVSELRRAKNTPSGSSTYYSLVGDINAYVQNAGLTAFFLTLKYGIN
TRTPALALSSLSGDIKKLGNLMRLYREKGDTAPYMTLLGDPDQMQFAPAEFALMYSFA
MGMASVLDKGTTKYQFARDFMNPGYWRLGVECAQQQSASIDEAMAAELRLSGNARKAL
ANAVSRMTEGVSQDLFEGGSASVTSGLGVQESQKPSKPSRGDEVRGPDGIPADEQRFL
DLMRSIAGNMRDSDAPPPPTPGASYQDREDDNLDGEPSSQWEL"
misc_feature 287..1594
/gene="NP"
/locus_tag="NZ93_gp1"
/note="Paramyxovirus nucleocapsid protein; Region:
Paramyxo_ncap; pfam00973"
/db_xref="CDD:395775"
gene 1868..3086
/gene="P"
/locus_tag="NZ93_gp2"
/db_xref="GeneID:20964447"
CDS 1868..3085
/gene="P"
/locus_tag="NZ93_gp2"
/codon_start=1
/product="phosphoprotein"
/protein_id="YP_009094169.1"
/db_xref="GeneID:20964447"
/translation="MATFTDQEIDEILSSSGAVIEEIITAEGKPKETVGRGSIPQDAA
KKKIKAWEIHNTKDQKPDSTTAASTGKDARNEQDGKAKQKNTEKSEDSSQDKTPSTDP
GDNIQEPDRNTDQISTPSASLLTMLDKIANRAAIKGLQHPEQPNQINVTPTKKGIMAG
NSAAKKETVDQALHQGLSSGTGSGLVIGENTPSHGRKRASYLSDGAIRSVPRSLQNPE
NSSVDAESAPQLADFVHATLGMMEMITQRMAKIEYSLDLVLKHASAVPIIKNDLQQIK
TTLAVLEGNIGMMKIMDPGNATISSLNDLRATTQLRPVLVAGPGDPAPYIQNDGSLAM
NQLAQPVRDRVNLVKSLTPPGPDLATEKETVRALINSRPMHPNSAKRLIEKLETASNI
DDIKKVKRLALNG"
misc_feature 1868..2032
/gene="P"
/locus_tag="NZ93_gp2"
/note="N-terminal region of Paramyxovirinae phosphoprotein
(P); Region: Soyouz_module; pfam14313"
/db_xref="CDD:316803"
misc_feature 2615..3076
/gene="P"
/locus_tag="NZ93_gp2"
/note="C-terminal domain of Measles virus phosphoprotein
and related proteins; Region: MEV_P-protein-C_like;
cl03936"
/db_xref="CDD:413868"
gene 3313..4407
/gene="M"
/locus_tag="NZ93_gp3"
/db_xref="GeneID:20964448"
CDS 3313..4407
/gene="M"
/locus_tag="NZ93_gp3"
/codon_start=1
/product="matrix protein"
/protein_id="YP_009094170.1"
/db_xref="GeneID:20964448"
/translation="MESSKAIGLDVDPSLPSSALLAFPVVLQDIGDGKKEITPQFRTQ
KIDIWSESKGDSVTITTYGFIYGPKGGERISGPISAGGNTEVLSAAMLCLGSVSYSHG
LPEIARAALGVVVSCKKSATDTERIIFTVHQASQLLQEAKVISNRYSSVAAIKCLKAP
ERVTSGISLEYRVTFVSLTVVPRGDVYKVPRPVLRLHSKHVFNISLSVKICIEVQPGH
PIAKTLIKQSDEYFADLFVHVGMISAIDKHGNKLAMERLENKIRRMDMSIGLLDIFGP
SIALKVRGKRTKVMTPFFSPRGTACYPISQTAPSIAKILWSNTGTLREARIIIQGGTN
KAIATTDDHFVTTTKLAHASKNGKFNPFKK"
misc_feature 3334..4362
/gene="M"
/locus_tag="NZ93_gp3"
/note="Viral matrix protein; Region: Matrix; pfam00661"
/db_xref="CDD:395535"
gene 4622..6262
/gene="F"
/locus_tag="NZ93_gp4"
/db_xref="GeneID:20964449"
CDS 4622..6262
/gene="F"
/locus_tag="NZ93_gp4"
/codon_start=1
/product="fusion protein"
/protein_id="YP_009094171.1"
/db_xref="GeneID:20964449"
/translation="MAIPVPSSTALMIFNILVSLAPASALDGRLLLGAGIVPTGDRQV
NVYTSSQTGIIALKLLPNLPKDKENCAEVSIRSYNETLTRILTPLAQSMAAIRGNSTV
STRGREPRLVGAIIGGVALGVATAAQITAATALIQANQNAENIARLAKGLAATNEAVT
DLTKGVGSLAIGVGKLQDYVNEQFNRTGEAIECLTIESRVGVQLSLYLTEVIGVFGDQ
ITSPALSDISIQALYNLAGGNLNVLLQKMGIEGTQLGSLINSGLIKGRPIMYDDGNKI
LGIQVTLPSVGRINGARATLLEAIAVATPKGNASPLIPRAVISVGSLVEELDMTPCVL
TPTDIFCTRILSYPLSDSLTTCLKGNLSSCVFSRTEGALSTPYVSVHGKIVANCKSVV
CRCVEPQQIISQNYGEALSLIDESLCRILELNGVILKMDGQFTSEYTKNITIDPVQVI
ISGPIDISSELSQVNQSLDSALENIKESNSYLSKVNVKLISSSAMITYIVITVICLIL
TFVALVLGIYSYTKIRSQQKTLIWMGNNIARSKEGNRF"
misc_feature 4700..6109
/gene="F"
/locus_tag="NZ93_gp4"
/note="Fusion glycoprotein F0; Region: Fusion_gly;
pfam00523"
/db_xref="CDD:395418"
gene 6503..8347
/gene="HN"
/locus_tag="NZ93_gp5"
/db_xref="GeneID:20964450"
CDS 6503..8347
/gene="HN"
/locus_tag="NZ93_gp5"
/codon_start=1
/product="hemagglutinin-neuraminidase"
/protein_id="YP_009094172.1"
/db_xref="GeneID:20964450"
/translation="MESATSQVSFENDKTSDRRTWRAVFRVLMIILALSSLCVTVAAL
IYSAKAAIPGNIDASEQRILSSVEAVQVPVSRLEDTSQKIYRQVILEAPVTQLNMETN
ILNAITSLSYQIDASANSSGCGAPVHDSDFTGGVGRELLQEAEVNLTIIRPSKFLEHL
NFIPAPTTGNGCTRIPSFDLGQTHWCYTHNVVLNGCRDRGHSFQYVALGILRTSATGS
VFLSTLRSVNLDDDRNRKSCSVSATPIGCEMLCSLVTETEEGDYDSIDPTPMVHGRLG
FDGKYREVDLSEKEIFADWRANYPAVGGGAFFGNRVWFPVYGGLKEGTQSERDAEKGY
AIYKRFNNTCPDDNTTQIANAKASYRPSRFGGRFIQQGILSFKVEGNLGSDPILSLTD
NSITLMGAEARVMNIENKLYLYQRGTSWFPSALVYPLDVANTAVKVRAPYIFDKFTRP
GGHPCSASSRCPNVCVTGVYTDAYPLVFSRSHDIVAVYGMQLAAGTARLDPQAAIWYG
NEMSTPTKVSSSTTKAAYTTSTCFKVTKTKRIYCISIAEIGNTLFGEFRIVPLLIEVQ
KTPLTRRSELRQQMPQPPIDLVIDNPFCAPSGNLSRKNAIDEYANSWP"
misc_feature 6980..8194
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Hemagglutinin-neuraminidase (HN) of parainfluenza
virus 5, Newcastle disease virus, and related
paramyxoviridiae; Region: HN; cd15469"
/db_xref="CDD:271233"
misc_feature order(6992..7009,7079..7081,7115..7117,7142..7144,
7148..7150,7184..7186,7190..7192,8150..8161,8171..8173,
8177..8185)
/gene="HN"
/locus_tag="NZ93_gp5"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:271233"
misc_feature 7019..7195
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Sialidase propeller 1 [structural motif]; Region:
Sialidase propeller 1"
/db_xref="CDD:271233"
misc_feature order(7022..7024,7274..7276,7397..7399,7451..7453,
7589..7594,7703..7705,7748..7750,7754..7756,7898..7900,
7994..7996,8078..8080)
/gene="HN"
/locus_tag="NZ93_gp5"
/note="receptor binding site [chemical binding]; other
site"
/db_xref="CDD:271233"
misc_feature 7202..7360
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Sialidase propeller 2 [structural motif]; Region:
Sialidase propeller 2"
/db_xref="CDD:271233"
misc_feature 7385..7690
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Sialidase propeller 3 [structural motif]; Region:
Sialidase propeller 3"
/db_xref="CDD:271233"
misc_feature 7694..7837
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Sialidase propeller 4 [structural motif]; Region:
Sialidase propeller 4"
/db_xref="CDD:271233"
misc_feature 7904..8053
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Sialidase propeller 5 [structural motif]; Region:
Sialidase propeller 5"
/db_xref="CDD:271233"
misc_feature 8072..8194
/gene="HN"
/locus_tag="NZ93_gp5"
/note="Sialidase propeller 6 [structural motif]; Region:
Sialidase propeller 6"
/db_xref="CDD:271233"
gene 8537..15145
/gene="L"
/locus_tag="NZ93_gp6"
/db_xref="GeneID:20964451"
CDS 8537..15145
/gene="L"
/locus_tag="NZ93_gp6"
/codon_start=1
/product="large polymerase protein"
/protein_id="YP_009094173.1"
/db_xref="GeneID:20964451"
/translation="MGSQGASSNAHNLILPESHLSSPIVKHKLLYYWRLTGLPLPSDD
QYDSLIITRDWKTILNSNKGEVSRMINLGRQTHSYLNHKLPVLSVTHPSTLKWLTSIR
STISDEKFATIEKFVRSHIRAYGARFSDLSNLVERKLYGESYLPGRSQEFKNLLSNPG
VWFHNQWTHSRNLWLHVKQLQRHLIVKCRERKNDQSIFTIEAIDGVVLITAELVSVVD
NRENRLTCLTQEMVLMYADLLEGRSMITLHCSLLPYLTPLGDKINDLLSLVDELSLTI
KGDIYEIVAILEAMAYSVVQLFEPNELFAGDFFSFNLSEIKTLLLLHFSTPIADSIIR
AISVIYSGISCDQGAEMLCVLRLWGHPLLTASTAAKAVRTQMCAPKIIDYDITMQVLS
FFKGTIINGYRKKNSGIWPKVNPSSIHSEIIHQLYLDSAEISHAIMLREYKALSLLEF
APCIDFDPISDLSMFLKDKAIARPSREWLSSFKTNLLTSADRPKKNSSQASNRLLLEF
LESSEFDPYKEMEYLNSLAYLDDDDVSVSYSLKEKEVKINGRIFAKLTKSLRNCQVMA
ESILAAEIAPFFQGNGVIQDSISLSKSMLAMSQMSFNCNKDRLLNTKRRISSIRESSN
NQKEKIRLATFLTTDLSKYCLNWRYQTIKPFARAINQLMGLEHFFEWIHLRLMNTTMY
VGDPYNPPQEVVTGDINDQPNDDIFIVSARGGIEGLCQKLWSMISISAINLAATRADC
RVACMVQGDNQVIASTKEIHCSDDPEQALRQLHELSDRFFRELIIVNHGLGHNLKLRE
TMRSNTFFVYSKRVFKDGRILSQLLKNAAKITLISGDLGEDTVASASDISGTIARICE
NGAGKDFSYLFNYYMLCVQFYFDQEFSIINRAGHDIRKTSLGNIELVHNYLLTPSQVG
GVNGLQYVRLYNRNIGDPGTTAFAEMKRLVSAQLLSRSVIENVMTRPPGSGNWATLCS
DPYSFNFDGTRSPSIVLKKHTQKVLFESCSNPLLAGVHQEDMDSEENDLARFLLDQEY
IHPRVAHVIMQASSIGRRKQIQGLLDTTSTIIKYALDKRPLSIKKSERIQNYPALHFQ
LYEEDVWSPTRRNSPLISSDMCSVSLADYCRNRSWSVLTGGRPILGVSNPDSLELISG
CIVSVSGSCRLCDSGDSQYTWFHLPGNIDLSDDAVGNPAIRVPYLGSKTQERRVASMA
KIRAMSPHTKAALRASSVLIWAFGDNEINWEAALAIANSRCAIDLDHLKVLAPLPTAG
NLQHRLDDGISQVAFTPASLYRVSSYVQISNDSQRLITEEGVRESNLIYQQIMLLGLA
SLEALFPLGTKYVTESLTLHLHSAHSCCLREASVSFPIALKGDPPSLRKEVQNRFMYD
PNPISLIEIPKLDITVFRSYELNLDSYSTIDLMEVLALSTGKLVGQSIISYDEETSIK
NDAIVVHDNSRNWISEFQNCDTVKLLEYAALEILLDCSYQMYYLRVIGFENIMLYLSD
TIRAMPGILLSNLAATVSHPSIFGRLYRSGMVSTQGSHQLASIDFISLSSQVLMKAIR
RMLTSFIQGDKPILLFPSVLDDTINDKFLQLIARYCCLVTLLFAKNDKVPKIRNMSPE
DKCRELTQYLVSPSTSRNYPHVDMNDLLTPKIDTFPANLYYMARKSLNLIREREDRDT
ILALIFPIPYHEELSHEKDPLTEVKDPFYTGECAFLIELRQGTDASRDLTKDELETVL
PVSRLSNQATADLRKYLFRGIGQASSSWYKIGQLFSIPEVRQSRGTNALYLGEGSGAL
MSLTELYLPHRMIYYHTQFDNAMNPPQRHFGPSPSQFLDSVVYQNMQADIPCALGFIQ
EFKVLWREVAAETDLTRGESVTYITTCIPCQGIGFLMVDAELPSDSPVSVIEEFITNI
MTIGLFCMADKGVMIIKALFTRTLEFNFMVNCMMLAADRVILISNGHMCRGDGECYII
GWFRSCVSAIHAGRVIQGAKQKARSGVCLLDPYQTQELVKLFVDQQAKVDEIITAPLE
NLTKYLTHNIDTALLAAGGRPTRPHGCDNAIISPADDIAFKEIIVKHIDTALKSIIYF
FDEGCLADTVFLLTPYNLSNRGKVSTLVHQTVRQLFEQELMRVRKEDLFTIDRMITLV
MLGRITLNDIIHWKEYLRRSSCKKYVRQRLGSEKVKTIFCYDSEVVLNRSTQKIYMKV
IGNAVKGYYGQD"
misc_feature 8585..11806
/gene="L"
/locus_tag="NZ93_gp6"
/note="Mononegavirales RNA dependent RNA polymerase;
Region: Mononeg_RNA_pol; pfam00946"
/db_xref="CDD:395756"
misc_feature 12170..15127
/gene="L"
/locus_tag="NZ93_gp6"
/note="mRNA capping enzyme, paramyxovirus family; Region:
paramyx_RNAcap; TIGR04198"
/db_xref="CDD:275046"
ORIGIN
1 accttacaga atctctgttt ggtacgttat aaagagatga aatatatgaa tttgtacggg
61 cagaacctgt gaatctcgag ttcgaccccg acggtctgag tctcgagatt ttggttcagc
121 catggcgtcc gtttttgaag aatatgacaa acttcttgag tctcagactc gccctagcaa
181 aggagtctca cctgctgaga aaggaggaac acttaaggtg gagctccctg tctttgtatg
241 caacagcaat gatgctgaaa ctcgatgggc atttgtctgc ttcgccttga gactcgcagt
301 tagcgactct tctaacaagc cgctgaggca gggagcgatg atctccttgc tctgtgcaca
361 ttccgaaacc atgcgcactc acgtggcgat ggcaggcagg aatggtgaaa catcaattgc
421 acttctggag atcgatgctt ttgtagacgg aatgccagta ttcaactctc gcagcggaat
481 tacagatgag aaagcccagc gcttcagtat gattgcggga gatttgccta gatcctgttc
541 taacaacacc cctttcatca atgctgatgc agagaatgat ccccctgagg atataacaga
601 tgcattggaa cggattctca ctgtccagac tcaactatgg gtcaccctag caaaggcgat
661 gacctcttat gagacagcag aggagtccga ggtgaggagg ataacaaaat atacacagca
721 agggagagta atcaagaagt tcctattatt ccccgtggtg aggagtagca ttcaagtaac
781 catcaggtca tcactggcag tgagagcatt catggtgtcc gagctaagac gtgcaaagaa
841 cacaccaagc ggatctagta cgtattacag ccttgtcggt gatatcaatg catatgttca
901 aaatgctggt cttacggcat tcttcctgac tctcaaatac gggatcaata ctcgcacgcc
961 agctctggcg cttagcagcc tatctggaga tatcaagaag ctgggtaacc tgatgagact
1021 ctacagagaa aaaggagaca ctgcgccgta tatgactctg ctgggagatc cagaccagat
1081 gcaatttgcg cccgcagagt tcgccctgat gtattcattt gcgatgggca tggcgtcagt
1141 cctggataaa gggactacaa aataccaatt tgccagagac ttcatgaatc cagggtactg
1201 gcgtctcggc gttgaatgcg cccaacaaca atctgccagc attgatgagg ccatggcggc
1261 tgagctccgc ctgagtggga acgcccgcaa ggcgctagct aatgctgtat ccagaatgac
1321 tgaaggcgta agccaggact tatttgaagg aggatccgca agtgtcacct caggactagg
1381 ggtgcaggaa tcacagaagc catccaagcc atcaagaggt gacgaagtca gaggacctga
1441 cggaattccc gctgatgaac agaggttcct agatctcatg aggagcattg cggggaatat
1501 gcgagatagc gatgctcccc ctcctcctac tcctggagca tcgtaccaag accgcgagga
1561 cgacaacctt gacggtgagc ccagctcgca atgggagctc taacatcact ctgcccacac
1621 accggcggat catcgcccga cccccacaac caacccaccc gcatcagcaa accacaaccc
1681 tccccatccc caatcacaat ctcactgccc aacaactgac caagctctac tcaattgcac
1741 gattctgcat ctattaagaa aaaaacaagc gtacgggcag aactggtgtt tcccgagtgg
1801 gctgtctacc cggctcacat tccagaaagt ctgcccaacc gcactcacaa caacccccat
1861 cccagtgatg gcgaccttca cggatcagga aatcgacgaa atattatcat ctagtggtgc
1921 tgtcatcgaa gaaatcatta ctgcagaagg caaacccaag gagacggtcg ggcgcggatc
1981 catccctcag gatgccgcca agaaaaagat aaaggcatgg gagatccaca acacaaaaga
2041 ccaaaagcca gactccacca ccgccgcaag caccgggaaa gacgcacgca acgaacagga
2101 cggcaaagcc aagcaaaaga atacagaaaa gtcagaggac agctcgcaag acaagacgcc
2161 ttcgaccgac cccggagaca acatccagga gcctgaccgc aacactgacc agatctctac
2221 cccatcagca agtcttttga ccatgcttga taagatagcc aacagggctg ccatcaaggg
2281 gctacaacat cctgagcaac caaaccaaat caacgtgaca ccgactaaaa aagggatcat
2341 ggcgggcaac tcagctgcaa agaaagaaac agtggaccaa gctcttcatc aggggctgtc
2401 atccgggacg ggttcaggcc tggtcatagg agagaacact ccatctcatg ggagaaagag
2461 ggcatcatat ctctcggatg gtgcaatccg atctgtgccc cggtcactgc agaaccccga
2521 aaattccagt gtagatgcgg agagtgcccc ccaacttgcc gactttgtgc acgcgacgct
2581 cgggatgatg gagatgataa cgcagagaat ggcaaagata gaatatagtc ttgatttagt
2641 attgaagcat gcttctgctg tcccaataat aaagaatgac ctgcagcaaa taaagacaac
2701 tttggcagtc cttgaaggca acataggtat gatgaaaata atggatccag gtaatgccac
2761 catctcgtcg cttaatgatt tacgtgccac aactcagctt agacctgtcc tagtggcagg
2821 ccctggcgat cctgctccgt acatacaaaa tgatgggagt ctagctatga accagttggc
2881 ccagccagtt agagacagag tcaacctggt gaaaagcctt actcctccag gaccagatct
2941 tgcaactgaa aaagaaacag tgagagctct catcaattct cgtccaatgc atccaaattc
3001 cgccaagcgc cttattgaga agcttgaaac agcttccaac attgacgata ttaagaaagt
3061 aaagcgtctg gcactcaatg gataaatgtg cagggcaaca cagcccagga tccccggaac
3121 cgcaagctca accgcccgcc cccgcataca tctgcccgca gccctcaaca atcaacacca
3181 aggactccac cacaatgcag caccttctca accctccact ctactgattc gacctgggca
3241 caatcccgtc ctttatccat aagattaaga aaaaatgtac gggcagaatt ggggttcgtc
3301 gaggccagag ccatggagag ctcaaaggcc attggactcg acgttgaccc tagcctgccc
3361 tccagtgcac tactggcctt cccggttgtt ctccaggaca tcggcgatgg gaagaaagaa
3421 atcactcctc agttccgcac ccaaaagatt gacatatgga gtgagagtaa aggggattct
3481 gtgacaatca ccacctatgg tttcatctac ggcccaaaag gaggagagcg catctccggt
3541 cccatatctg cagggggcaa cactgaagtc ctgagtgcag caatgttgtg tctcggaagc
3601 gtcagctatt ctcatggcct ccctgagatt gcaagagccg ctctaggagt cgtagtatca
3661 tgcaagaaaa gcgctactga tactgaaaga attatattta ctgtccatca agcgtctcaa
3721 ctgctgcagg aggcaaaagt gatatcaaac cgatattcat cagtggcagc gattaagtgc
3781 ctaaaggcac ctgagagagt cacgagcggt atatccttgg aatatcgagt tacttttgtc
3841 tctcttactg tagtccctcg tggagatgtg tataaagtgc cacgtcctgt cctgcgcctc
3901 catagcaagc atgtctttaa catttctttg agtgttaaga tatgcataga agtgcagcca
3961 ggtcacccta ttgctaagac cctcatcaag cagagtgatg aatacttcgc agacttgttt
4021 gtgcatgttg gcatgatatc agccatagac aaacacggga acaaattagc catggagaga
4081 cttgagaaca aaatcaggcg aatggacatg tcgataggac tcctggatat attcggaccg
4141 agtatagcac tcaaggtcag agggaagagg accaaagtca tgacaccctt tttttcacct
4201 cgtgggactg cttgctaccc aatatctcaa actgccccta gcatagcaaa aattctgtgg
4261 agcaacaccg ggaccctcag agaagctaga ataataatcc agggcggaac taacaaagca
4321 atagccacaa ctgatgatca ttttgtaact accacaaagc tggctcatgc ctcaaaaaat
4381 gggaaattca atccttttaa gaaatgaact cacagcatca tcaatcccat catttcttca
4441 tcataccagt cacgaggcta ataaatcagc actaatcatt aaccgatgac agagtgaatc
4501 ccaaatcaag aatcatgtga ttaaacctag ataatttcca aacctcatta agaaaaaact
4561 gatactgcca cgggcagaac tgggattcag acgtacgtgg ccactccact gcaggaacaa
4621 catggccatc ccagtgccct cttcgaccgc tctgatgatc ttcaacattc tagtgtccct
4681 cgcccccgcc tccgctctgg atggcagact gttgttagga gcaggtatcg tacctacggg
4741 agacagacag gtaaatgtgt atacttcatc tcaaaccggt ataattgcct taaaattgct
4801 gcccaacctc ccaaaggata aggagaattg cgctgaggtg tctatcagat cctacaacga
4861 gactctgacc cgcatcctca cccctctcgc tcaatccatg gcagccataa ggggtaattc
4921 aacagtatca actcgtggaa gagagccaag actagtgggt gccatcatag gaggcgtagc
4981 tctaggtgta gctacggcag cacagatcac agcggcaacg gcccttatcc aagccaatca
5041 aaatgcagag aacattgcaa gacttgccaa aggtctagca gctaccaatg aggcagtgac
5101 ggatttaacg aaaggagtgg gctctcttgc tattggggtt ggaaagttac aggattatgt
5161 aaatgagcaa tttaatagga cgggagaggc aatcgaatgt ttgacgatcg aatctagagt
5221 aggtgtccag ctcagtctct atctaacaga ggttattgga gtcttcggtg atcagatcac
5281 atctccagca ttatctgata tcagtattca ggcattatac aatctggctg gagggaactt
5341 gaacgtcttg ctgcagaaga tgggtattga agggacacag ctaggctcct taatcaacag
5401 cggattgata aaaggcagac caatcatgta tgatgatggt aacaaaattt taggtatcca
5461 agtaactctt ccatcagtgg gtaggatcaa tggcgcacga gcaactctac ttgaggcaat
5521 tgcggtggct actcctaaag ggaatgctag cccattaata cctagagctg ttatctcagt
5581 gggatcgcta gtggaagaat tagatatgac tccatgcgtg ctgactccaa cagacatctt
5641 ttgcaccagg atcttgtctt atccattaag tgattctctc accacttgtc tcaaagggaa
5701 tctttcgtct tgcgtcttct cacgtacgga aggggcatta tcgacacctt atgtttctgt
5761 gcatggtaag attgttgcca attgtaagtc tgtggtttgc cgatgtgtgg agccacaaca
5821 aatcatatcc caaaactatg gggaggccct tagcctgata gatgagtccc tatgtaggat
5881 cttagaacta aacggagtga tccttaagat ggacggacag ttcacatcag aatacacaaa
5941 aaacataact atagatcctg tgcaggtcat aatatctgga ccgatcgata tatcttctga
6001 gctttcgcag gtcaaccaat cactagatag cgcactggaa aatataaaag agagcaattc
6061 atacctgtca aaagtgaatg tgaagctgat cagctcctcg gccatgatca cgtacattgt
6121 gataactgtg atttgcctga ttttgacttt cgtagcgtta gtccttggga tatactcata
6181 tacaaaaatc aggtctcaac agaagactct gatatggatg ggtaataaca ttgcgaggtc
6241 aaaagagggg aaccggtttt aagtgatgga tgcatagcca cacaaatcag aaatgatata
6301 ctagttaaca gtgatcatga ggtaatttgt cattgttcaa tgaaaaaaaa ctaatgataa
6361 gacgatcatg gaattaaaaa gaaactagga agggaataac aaaggaccag tacgggcaga
6421 acgagagagt ctcgactcgc cgtctgacga cagccgagtc gagaacccga tcaaactgtc
6481 tgcccacgag agctatctcg ccatggagag tgcaaccagc caagtgtcct ttgaaaatga
6541 caaaacctct gatcgtcgga cttggcgagc agtatttaga gtactgatga taatactcgc
6601 tcttagtagc ctatgtgtaa ctgtagcagc tcttatatac tcagcgaagg ccgcaatccc
6661 tgggaacatc gatgcatctg aacaaaggat attatcatcc gttgaggccg ttcaggtgcc
6721 cgtatcaagg ttagaagaca ccagtcagaa gatataccgc caggtcattc tcgaggcgcc
6781 ggtaactcag ctcaacatgg agacgaatat tctaaatgct attacatccc tttcatatca
6841 aattgatgct tcagccaact cttctggttg cggtgcccct gtccatgact ctgacttcac
6901 agggggtgtc ggtcgagagc tacttcaaga ggcagaagtt aatctgacca taatcaggcc
6961 ctccaaattc ttggagcact taaacttcat accagccccg acaacaggga atggctgcac
7021 aaggatacca tcgtttgatc taggccaaac tcattggtgc tacacacaca acgtcgtgct
7081 caatggctgc agagaccgcg gccactcttt tcaatatgtt gcactaggca tactcaggac
7141 atcagctaca gggtcagtat tcttatcaac actccgatct gtaaatttag acgacgaccg
7201 taacagaaag tcatgtagtg taagtgcaac cccgataggc tgcgagatgc tctgttctct
7261 tgtcacagag actgaagaag gagattatga tagcatcgac ccgaccccta tggtgcatgg
7321 caggttagga tttgatggca aatataggga agtggacctt agcgaaaagg agatattcgc
7381 tgactggcgc gccaattatc ctgctgttgg cggtggcgct ttttttggta atcgtgtatg
7441 gttccctgtt tatggaggtc tgaaggaagg aacccaaagt gagagagatg cagagaaagg
7501 ttatgcaata tataaacgct tcaataacac ttgccctgac gataatacaa ctcaaatcgc
7561 gaatgctaaa gcatcatatc ggccatctcg atttggcgga cgatttatcc aacagggtat
7621 cctctctttt aaagttgaag ggaacttagg atcagatccg atcctcagcc tgactgacaa
7681 ctcaatcaca ttgatgggtg ccgaggcacg tgtgatgaat attgagaata aactatacct
7741 ctatcagaga ggtacttctt ggtttccatc tgccttagta taccccttgg atgtagctaa
7801 tacagctgta aaagtgcggg cgccatacat ttttgacaaa ttcactaggc ccggaggaca
7861 tccatgcagc gccagttcac ggtgccctaa cgtatgcgtc acaggggttt atacggatgc
7921 ctatccactt gtattttcaa ggagtcatga cattgtggca gtctacggta tgcagttggc
7981 ggcgggcact gcacgacttg atcctcaggc agcaatatgg tatgggaacg agatgagtac
8041 acctactaaa gtaagtagct caactactaa agctgcctat actacttcca catgttttaa
8101 ggtgacaaaa actaagagaa tctactgtat aagtatagca gaaataggga acacactctt
8161 tggcgagttt aggatagtgc cactattaat cgaagtacaa aagactcctc tcactaggag
8221 aagcgagctc cggcaacaaa tgccccaacc tcccatcgat ttggttattg acaatccgtt
8281 ctgtgcgccc tctggtaact tgagcagaaa gaatgccatt gacgagtatg ccaattcatg
8341 gccatagaat tcgaaaagaa ggaaataact atctgtctcc ctgactgcgt ttacctatac
8401 tatatcaata aaatcgacaa agaaatctga gcatagtaaa atctgactaa cttttgggac
8461 atgggtctct attaagaaaa aaataattaa agaataagca gacccactct tgaaagggag
8521 agtatacggg cagaagatgg ggtcccaagg ggcctcttcc aatgcccata atctaattct
8581 tccagaatca cacctctctt caccgattgt aaaacacaag ctactctatt attggagatt
8641 aactgggttg cccctcccgt ctgatgacca gtatgactcg ctcattatta cacgtgattg
8701 gaaaaccatc ttaaactcca ataaaggaga agtcagccgc atgataaact taggccgtca
8761 gactcactct tatcttaatc ataaattgcc tgtgctctct gtaacacatc cttctacttt
8821 aaaatggctc acttctatca ggtcaacaat ttcagacgaa aagttcgcca ctattgagaa
8881 atttgtgagg tcccatatta gagcatacgg tgccagattt tctgaccttt ctaatctagt
8941 cgagagaaag ttatacggag agtcgtattt acctggtcga tctcaggaat tcaagaatct
9001 tttatccaat ccaggggtct ggtttcataa tcagtggaca cattctcgga acctttggct
9061 ccatgtgaaa caactgcaac ggcacttgat tgtgaagtgt agggaacgaa agaatgatca
9121 gtcgattttc accattgaag caattgatgg tgtggtcttg ataacagcgg aattagtttc
9181 agtggttgac aatagagaga atcgattgac ctgcctgact caagaaatgg ttcttatgta
9241 tgctgatctc ttggaagggc gaagcatgat cacactgcac tgttccttac taccttacct
9301 aacaccactc ggagacaaaa taaacgatct actctctcta gtagacgaat tatcgttgac
9361 cattaaagga gatatttatg agatagtagc aatcttggaa gcaatggcat acagtgtggt
9421 ccagttattt gaacctaatg agctgtttgc aggagacttc ttctccttta acttatcaga
9481 gatcaagaca ttactcttgc ttcatttttc tactcctatt gcagactcca ttatccgcgc
9541 tattagtgtg atttatagcg gtatctcttg tgatcaagga gcagagatgt tgtgtgtatt
9601 gaggttatgg ggtcacccat tgctcactgc ctcgactgct gcaaaagcag taagaaccca
9661 aatgtgtgcc cccaaaatca tagactatga tataacaatg caggtgctgt cattctttaa
9721 aggtaccata ataaacggtt atcgaaagaa gaattcaggg atctggccta aagtcaatcc
9781 ttcatctatc cattcggaga tcatacatca attgtactta gattctgcag agattagcca
9841 tgcaataatg ttacgcgaat acaaggccct ttcgttgtta gagtttgctc catgcattga
9901 ctttgatcca ataagtgatc ttagcatgtt tttaaaagac aaggcaatcg caagaccttc
9961 cagagagtgg ttgtcatctt tcaagactaa tttactaacc tcggcagata ggcccaaaaa
10021 gaactcaagc caggcaagta atcgattact attggaattt ctagaatcat cggaatttga
10081 cccatataag gagatggaat atcttaactc tcttgcttat ctcgatgatg atgatgtgtc
10141 agtctcttac tcactcaagg agaaagaagt caaaatcaac ggtcggattt ttgcaaagct
10201 cacaaaaagc ctacgaaatt gtcaagtgat ggcagagagt atcttagcag cggaaatagc
10261 tccttttttt caagggaatg gagtgattca ggacagcata tccttatcta aaagcatgtt
10321 ggcaatgagc cagatgtcat ttaactgcaa taaagaccgc cttcttaaca cgaaaagacg
10381 gatatcatca atccgagagt cgtctaataa tcaaaaagag aagattagat tagccacatt
10441 cttaacgaca gatctctcaa aatattgcct aaattggaga taccagacaa taaaaccatt
10501 cgcaagagca atcaaccagc ttatgggtct cgagcacttc tttgaatgga tacatttgcg
10561 cctgatgaat actactatgt atgtaggaga cccgtacaac cctcctcagg aagtagtgac
10621 aggggatatt aacgatcagc ctaatgatga catcttcata gtaagtgcaa gaggtggaat
10681 agagggtctt tgccaaaaac tctggagtat gatctcaata tcagctatca acctggcagc
10741 tacgagagca gactgcagag tagcctgcat ggtacaaggg gacaaccaag taattgcatc
10801 cacgaaagaa atccattgca gtgatgaccc agagcaggcc ctccgtcaat tacacgaatt
10861 aagcgaccgc ttctttagag agctgattat cgtaaatcat ggattagggc ataacctcaa
10921 attgagagaa acaatgagat ccaacacatt ctttgtgtac agtaaacgag tctttaagga
10981 tggtagaatc ttgagtcagc tcctaaagaa cgctgcaaaa atcacactta tctctggtga
11041 tctgggtgaa gacacagtgg catctgcaag tgacatttca ggtacaattg ctcggatctg
11101 tgagaacggt gctggaaagg acttttctta cctgtttaat tactatatgt tatgtgttca
11161 attttatttt gaccaagaat tctctattat caatcgggca ggtcacgata ttagaaagac
11221 atctcttgga aatattgaat tagttcataa ttatttgctc actccatccc aagttggcgg
11281 cgtcaatggt ttgcaatatg tgaggttgta caatagaaat ataggggatc cgggaaccac
11341 agcatttgca gagatgaaac gcctggtgag tgcacaattg ttatcacgca gtgtgattga
11401 gaatgtgatg acccgaccac cgggatctgg caattgggcg actctatgca gcgatcctta
11461 ctcatttaac ttcgatggaa cccgcagccc gagcattgtc cttaagaaac acactcaaaa
11521 agtattattt gagtcgtgtt ctaatccctt actagcaggt gtgcaccaag aggatatgga
11581 ctcagaagag aatgaccttg ctagattctt gcttgatcaa gaatacattc atcctagggt
11641 agcgcatgtg ataatgcagg ctagttccat cgggcgaaga aaacagattc aagggttatt
11701 agatacaact tcaaccatca ttaagtatgc gctggacaag aggcctctca gtatcaagaa
11761 gtcagagagg atacaaaatt atccggcctt gcatttccaa ctgtacgaag aggatgtctg
11821 gagcccgacc cgcaggaatt caccactgat atcctctgat atgtgctcag tgtctcttgc
11881 tgattactgt cgtaatagaa gttggtctgt gcttaccggt ggtcgtccta tactaggcgt
11941 atccaatcca gattcccttg agttgatatc tggttgcata gtttccgtga gtgggagctg
12001 taggctctgt gatagtggcg actcacaata cacttggttt catctgccag gtaatatcga
12061 ccttagtgat gatgcagtcg ggaatccagc tataagagtt ccttatctag gatcaaaaac
12121 tcaagaacga agagtggcat ctatggcgaa gatcagagca atgtctccac acacgaaggc
12181 agcccttaga gcatcttctg tattgatctg ggcgttcgga gataatgaga taaattggga
12241 agctgcactg gccatagcta atagcagatg tgcgattgac ttagatcacc tcaaagtgtt
12301 ggcccctctg cctacagcag gaaatttaca acacaggttg gatgacggga tatctcaggt
12361 agcttttacc cctgcttcat tgtacagagt ctcatcctac gtgcaaatat ccaatgactc
12421 acagcgactg attacagaag aaggtgtgcg tgaaagcaat cttatttatc agcaaatcat
12481 gttactcgga ttggcatctc ttgaggcact cttcccgtta ggaactaaat atgtcacaga
12541 gagcctcaca ctccatcttc atagtgccca cagttgttgc ttgagagagg catccgtatc
12601 cttcccaatt gcattgaaag gtgatccgcc ttctcttcgt aaagaagtgc agaacagatt
12661 tatgtatgat cctaacccaa tatctctcat agagatccca aaattagata tcactgtttt
12721 tagatcgtat gagttgaacc ttgactctta ttcgacaatt gatctgatgg aggtattagc
12781 actctctacg gggaagttgg tgggccagtc aataattagt tatgatgaag agacatccat
12841 caaaaatgat gcaatagtgg ttcatgacaa ttccagaaat tggatcagcg agttccaaaa
12901 ttgcgacaca gtaaaattat tagagtatgc cgcactggag attctcctgg attgctcata
12961 ccagatgtat tacttgagag tgatcggctt tgaaaatata atgctttacc tatcagacac
13021 catcagggct atgcccggaa tattgttatc caatttggca gccactgttt cgcatcctag
13081 tattttcggc cgtctctata ggagcggaat ggttagtaca caagggtctc accaattggc
13141 atctattgac ttcataagtc tgtcatctca agtcctaatg aaagctatca gaaggatgtt
13201 gactagcttc atacaaggcg acaaacctat actattattc ccgtcagttc tagatgacac
13261 cataaatgat aagttcttac aactaatagc tagatactgc tgcttagtga cgctcttatt
13321 cgctaaaaat gacaaggtac caaaaatcag gaacatgtct cctgaagaca aatgccgtga
13381 actcactcag tatcttgtgt caccaagcac tagtcggaat tatccccatg ttgacatgaa
13441 tgaccttctc acaccaaaga tagatacatt ccctgcgaac ttatattata tggctaggaa
13501 gagtcttaat ctgataaggg aaagagagga tcgcgataca attttggctc tcatcttccc
13561 gatcccatac cacgaagagc tctcacatga aaaagacccg ttaacggaag tgaaggatcc
13621 cttttatacg ggagagtgtg cattcctaat tgaactaaga caggggaccg atgcatcaag
13681 ggatctcaca aaagatgaac tagagacagt gttaccagtg agtagattat ccaaccaggc
13741 aacggcggac ttacggaaat accttttcag agggattgga caggcatctt catcatggta
13801 caaaattgga caattattct caatccctga agtcagacag agtcgaggca cgaatgcctt
13861 atacttgggt gaaggaagtg gtgctctcat gtccttgact gagttatacc tcccccatcg
13921 aatgatatac tatcacacac agtttgataa tgccatgaat cccccgcaga gacactttgg
13981 accttctcca tctcagtttt tagactcagt ggtgtaccag aacatgcaag cagacattcc
14041 atgtgcatta ggattcatac aagagttcaa agtgttatgg agagaggtgg ctgcagaaac
14101 ggacttgacc agaggtgaga gtgttacata tataacaact tgtatcccgt gccagggaat
14161 cggattcctt atggtagatg ctgagttgcc ttctgattcc cctgttagtg tgatagagga
14221 attcataacc aatatcatga ccataggatt attttgtatg gctgataaag gagtgatgat
14281 aatcaaagcc ttatttacta ggactttaga gttcaatttt atggtgaact gcatgatgct
14341 ggcagccgat cgagttatac tcattagtaa tggacacatg tgtcgggggg acggagaatg
14401 ctacataata ggttggttta ggagctgtgt atctgcaata cacgctggta gggttatcca
14461 aggagccaaa cagaaggcca ggagcggagt gtgccttttg gacccctatc agactcagga
14521 attagtaaaa ttatttgtcg accaacaggc aaaggtagat gaaataatta cagcgccttt
14581 agagaatctc acaaagtact tgactcacaa tattgacaca gctcttcttg ctgcaggagg
14641 gagaccaact cgtcctcatg gatgtgacaa tgctatcata agccctgcgg atgatatagc
14701 cttcaaggag ataattgtaa agcatattga caccgcacta aaaagtatca tatacttttt
14761 tgacgaaggg tgccttgcag atactgtttt tcttctgacc ccctataatc tgagcaacag
14821 ggggaaggtt tcaaccttag tccaccaaac tgtgaggcag ttatttgaac aagagttgat
14881 gcgagtaaga aaagaagatt tatttacgat tgacagaatg atcactctag taatgctcgg
14941 acgcattacc ctgaatgata tcattcactg gaaggaatac ttgagacgct cttcttgtaa
15001 gaaatacgtc agacagcgcc ttggttcaga aaaggttaag actatctttt gctatgattc
15061 tgaggtcgta ttaaacaggt ctactcagaa aatttacatg aaggtgattg ggaatgcagt
15121 taaagggtat tacgggcaag attaatagag ttaagtataa taatagttat ctatagaatg
15181 tatagttata ataatcgtaa gcatagtgta gatttagact ttcgaagtcg tcttctaatc
15241 aattagaaaa aacatggaaa gtaacccgag aagaaactac taaagacgta atccaccaaa
15301 tctttgtttg gt
//
