LOCUS NC_030401 10158 bp RNA linear VRL 24-MAY-2019
DEFINITION Hanko virus polyprotein gene, complete cds.
ACCESSION NC_030401
VERSION NC_030401.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Hanko virus
ORGANISM Hanko virus
Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Flasuviricetes;
Amarillovirales; Flaviviridae; Flavivirus.
REFERENCE 1 (bases 1 to 10158)
AUTHORS Blitvich,B.J. and Firth,A.E.
TITLE Insect-specific flaviviruses: a systematic review of their
discovery, host range, mode of transmission, superinfection
exclusion potential and genomic organization
JOURNAL Viruses 7 (4), 1927-1959 (2015)
PUBMED 25866904
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 10158)
AUTHORS Huhtamo,E., Moureau,G., Cook,S., Julkunen,O., Putkuri,N.,
Kurkela,S., Uzcategui,N.Y., Harbach,R.E., Gould,E.A., Vapalahti,O.
and de Lamballerie,X.
TITLE Novel insect-specific flavivirus isolated from northern Europe
JOURNAL Virology 433 (2), 471-478 (2012)
PUBMED 22999256
REFERENCE 3 (bases 1 to 10158)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (30-JUN-2016) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 4 (bases 1 to 10158)
AUTHORS Huhtamo,E., Moureau,G., Cook,S., Julkunen,O., Putkuri,N.,
Kurkela,S., Uzcategui,N.Y., Harbach,R.E., Gould,E.A., Vapalahti,O.
and de Lamballerie,X.
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2011) Department of Virology, University of
Helsinki, POB 21, Haartman Institute, Haartmaninkatu 3, Helsinki,
Uusimaa FIN-00290, Finland
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to JQ268258.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..10158
/organism="Hanko virus"
/mol_type="genomic RNA"
/host="mosquito"
/db_xref="taxon:1125677"
/country="Finland"
/collection_date="Aug-2005"
gene 1..10158
/gene="POLY"
/locus_tag="BBA73_gp1"
/db_xref="GeneID:28218218"
CDS 1..10158
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="Mature peptides were annotated according to
predicted cleavage sites published in PMID 25866904"
/codon_start=1
/product="polyprotein"
/protein_id="YP_009259489.1"
/db_xref="GeneID:28218218"
/translation="MHRLRDLLPDKGKKNRSPAVRRQGGIAKGVDRKIIPSSGSKERK
VGTERKAKRVVARMKPNWLSFGTGNQRSMWRQIFSVDLMEGLLLFIALMSNLYERVQR
DIADLKRRVTRLEKERSHPRKVPIMLLCGLIVVTGLSIELALDENGDVEMWADNQNIT
NYAHLTKVPADVCIVGMVVTKHCPKVEKMTDLSDIDCGSTWVEFILTYTRCMILERET
RQKVDDKSPLTRFKEDLSTLETEAFQMLKRHAFSTILALITLAIVLKWPIWVVVLLGF
LAWNVVKGEFVEPFLVLKHDHNTMLTTRLYPGEIAHVATPTGLLDIRVGNAGIFGSQL
FRELLRDCKVNASYSTDICPGGSQLDMEAIQGPGRVCFTAPYNRGWGTGCFKWGIGAV
ATCVELNCSSSMGVHLLTNSAIVANVSVGFHSTNDTKMLVPDAPTTLKFGKLGTVTMN
CRLGNDRIATSYYHVTDGLSTGLFLKAQIDAWSGPFRMAGLTGGFDKIVKWGQVTPNE
IKVKRISEMELDWDNAITTHDGFVNTYFWCQIAVNKLVVGTFASCKSEAQTVFSQSPW
GFEGIAEITLKEAQKSICSLPLSCVGCSLLSTKVVFLETTTKAAVHVGCGNGTSVLTV
GTTPVSIDCVVTPLSQVWRLVSHVTGRYTKLGFGGVGGVFHDLLQACGWVFTWDSWKV
ILILGGLVVLFIVFDRKIVLVVMIASAVVYVKADVGCGIDFDRKTYTCGRGLFIWKGI
GTYPTSDHSVEFESYDFLAAYLKEQFKAERKVCIVCEDLVQCEAARKAASAIYRDLGH
PFVYVNVSSSYGKSFAEVPKRVHTVSVGVDVVEMAMMVSNNKPSGPFGELPTSVVSWS
TAPETEDHLVLRVVTSSTPYRQVCGKAIGFQYDFVGFRRAVYGSNVQLKISKKVSIEC
PTYLAGMAVKNDRTVFTDGMFWMSSRKDNDSYAIDELEMEQSHRCVWPNQYTPDMVQD
PRDNDLFVPPEWGGPRSKANHIPGYKMQISFPWDKAPIKLVDGSVPGTIVTQISKCEG
RGIPVMVDPAVNPNWCCKSCTRIFHFEVDGKLYYPMEIRPDVKEAKNKRSPVIEEPLG
DDTAETVTDWLSKTYRVPSTNAEDFLNPKLILTKANAMVGEVVNLLCLALAMQVVTHT
WKHKSLARFHLCCLLFLLFGVPTVFGFVGIYTWMNILPISHGSARMCNLTIHLWAVLQ
HRSSSMFLWGQTLRSQFQTSLAGQMLLLTMQMLHQAIYAHSSMLGWGIEVFLSVIVMQ
NLYTVVDHIHPRLIAYCLLFGWRTGLCIGCGFLLTFLMKRWMTIVAASPSAGGWRSGY
RALCSSTLTLLFTAVGVAGVIASDYGGYPGASAAIAACLIAGIKMTDFLATRLSLEFV
STGQFPEGTTIEKEKETYDGTFRASFTVEGIKLLDHTEPVPLVFAISYVALGAVTCKI
HPGLGVIYAVAMVATNLPSLLQVYVLSICTNAFRSDELIGTAIASVEPELSKDFGSIP
DGIYRVNNHGIAFKSHRGVGIVKNGVFHTLMHITMNEPVTWQNKLVGPYMGNSLKDYL
CYGGNWQLPSFDPTDEVGIMVCKSDRSVEYKRHEVGKIDVDGVPHMYFTKDYGRGTSG
SPIFVNGEPVALYGFGFFLYNTYRSLVVPVPRADIEAGESAEGLVEENSSIVNKFFVD
WHPGKGKTRKVIVKHVLEAIKDSRRILVLAPTRVVMAEIMKALEESTHKAISKSMSHT
SHNGVTVACHATFTDFILAHGLSRFKAHEVIMDECHFLDPRSIAARGILEHLAQKRGV
KVVFMSATIPGREPSIGSNYEIAEQALQFPREVTSKWIGDVSEGKTVVFVPSHKVGDK
LALGSPHSISLHRNNFNTNYSIARSDDIKYVYTTDISEMGANFNATTVVDFRVAIKPK
ILNECEVTLAPTPITRSSMVQRRGRVGRQHPGKYIYPANKGTEEAANDLACWTEAQMI
LDQLDLTMMAEEAPYSNHPGAYKLVGKSFQVFKKLLEDKDDIPIWLSWKWADNTEHQY
AALFEGERLENVPRMVNTREYASIEYKPKFVDARFERLSWEQRKLAIQFYMGTRSFLS
VGLFYKVLSQIIQAGVVNTAWKKIGDVGLVFMEGGDPHSKDESIVAWTILIGGMLCVI
VLMICVWGLRIVMRMVFGSREKHSSVPTLLADSQPYLVCLVPVATHLAGVPVPVTIVV
FVILFLTFPLIYRSAGQRSYVDIDLVKWILVGGGFIIGVICWELRLLPNITSDLQSIS
HGRTRTDSSGSRQESDWGFSWMDFAQPVPTSVELTSVVITTFTMALFLNQIVGWSYEA
DWLKSYFDHKGVGHIMGGFRLDTISWGSALSSLLGTLSYASWGAIIIGMGGATIYFFF
MVKMLKWNFTGGSTIGLENNTMRQDRETGLNRRPFHDNRRSLLYGVVIFECALWVFCF
MSVFDLVIASCVSTYCVWIIFNASSEHHRNVDLGSVCSFVGLLYATCPSQKCVQVLIR
FALSRLNITTRSLEKSATGGLGHRWKKILNAMSQDDFNSYRLYGVDETDKGDYVSRGG
LKLRELTLKYGWKPEGICVDLGCGRGGWSQHLAMDPRVTRIEAYTLGGSTRENPQPVK
TLGHNLIRFKTGVNVYNMVPTFANTIVCDIGESDPKTEVEASRTIRVLSTLGTWLEKN
PNAEFVCKVLCPYPVEVLRLLETFQHKYGGRIVRSTFSRNSSAEMYYISGGRNNIVKV
VFTTLHALIARLRTKPDKIVKEPIRLPVGTRSDPGHKVKDMNPKMIATRVEKLRNEHK
DTWFIDSNHPYQSFRYVGSYVTDDINPGGQTVNPLMRKMMWPWELVGGVVNFMMTDVS
TYAQQKVLREKVDTLSPEPPKNIKTINRLITEFQIRSYTKRGLRPRILTMEQYADNVK
SSAAIGSWSSDVPWNNVRAALSDANFHALVDEERRLHLAGDCRMCVYNTMGKKEKKPA
SMGVAKGSRTIWYMWLGSRFLEYEALGFLNEDHWVSRENLACGVGGVGVNYFGYYLQE
ISKKGKFFIADDIAGWDTRINESDLADEEHLIMSMISDTRHRALATAVFKFAYQNIVA
LFPRNHPGFGSGTVMDVVARTDQRGSGQVVTYALNTITNAKIQLGRMIEAEGLLGAPE
SVITKWLDDNGEDRLSAMVVAGDDVVVATNNDKFSHSLDYLNLNGKIRKDIDPSLPSR
VQTNWETVEFCSHHYHPMTLRDGRRIIVPCRDQNEVIGRGRIQKGGLVTIADSACLAK
AYGQMWALYFFHRRDLRMAFMAITSSVPVDWFPEGRTSWSIHQNKEWMTTEDMLRVWN
TVWIHDNNWMEDKTEVTAWKDIPYLPKSIDIKCGSQIGSKDRATWSRELPSTVMAIRR
ILDHETRMENVYKDFLSGMGRFREENDPMAAGVRF"
mat_peptide 1..411
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="anchored capsid protein C"
/protein_id="YP_009268615.1"
mat_peptide 1..354
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="capsid protein C"
/protein_id="YP_009268616.1"
mat_peptide 412..852
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="membrane glycoprotein precursor prM"
/protein_id="YP_009268617.1"
mat_peptide 412..657
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="protein pr"
/protein_id="YP_009268618.1"
mat_peptide 658..852
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="membrane glycoprotein M"
/protein_id="YP_009268619.1"
mat_peptide 853..2145
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="envelope protein E"
/protein_id="YP_009268620.1"
mat_peptide 2146..3321
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="nonstructural protein NS1"
/protein_id="YP_009268621.1"
misc_feature 2152..3222
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="Flavivirus non-structural Protein NS1; Region:
Flavi_NS1; pfam00948"
/db_xref="CDD:279316"
mat_peptide 3322..3963
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="nonstructural protein NS2A"
/protein_id="YP_009268622.1"
mat_peptide 3964..4410
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="nonstructural protein NS2B"
/protein_id="YP_009268623.1"
mat_peptide 4411..6210
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="nonstructural protein NS3"
/protein_id="YP_009268624.1"
misc_feature 4483..4872
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="Peptidase S7, Flavivirus NS3 serine protease;
Region: Peptidase_S7; cl29867"
/db_xref="CDD:453075"
misc_feature 4996..5412
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="N-terminal helicase domain of the DEAD-box helicase
superfamily; Region: DEAD-like_helicase_N; cl28899"
/db_xref="CDD:452890"
misc_feature order(5011..5031,5275..5277)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="putative ATP binding site [chemical binding]; other
site"
/db_xref="CDD:350670"
misc_feature 5416..5808
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="C-terminal helicase domain of the DEAD-like
helicases; Region: DEAD-like_helicase_C; cl38915"
/db_xref="CDD:365791"
misc_feature 5773..5775
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:350171"
mat_peptide 6211..6624
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="nonstructural protein NS4A"
/protein_id="YP_009268625.1"
mat_peptide 6625..6693
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="protein 2K"
/protein_id="YP_009268626.1"
mat_peptide 6694..7473
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="nonstructural protein NS4B"
/protein_id="YP_009268627.1"
mat_peptide 7474..10155
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="RNA-dependent RNA polymerase NS5"
/protein_id="YP_009268628.1"
misc_feature 7501..8163
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="Cap-0 specific (nucleoside-2'-O-)-methyltransferase
of flaviviridae; Region: capping_2-OMTase_Flaviviridae;
cd20761"
/db_xref="CDD:394920"
misc_feature order(7516..7518,7525..7530,7534..7536,7885..7890,
8083..8085)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="nucleic acid substrate binding site [nucleotide
binding]; other site"
/db_xref="CDD:394920"
misc_feature order(7678..7686,7693..7698,7747..7752,7825..7830,
7873..7878)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="SAM binding site [chemical binding]; other site"
/db_xref="CDD:394920"
misc_feature order(7873..7875,7984..7986,8092..8094)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="active site"
/db_xref="CDD:394920"
misc_feature 8401..10098
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="conserved catalytic core domain of RNA-dependent
RNA polymerase (RdRp) from the positive-sense
single-stranded RNA [(+)ssRNA] viruses and closely related
viruses; Region: ps-ssRNAv_RdRp-like; cl40470"
/db_xref="CDD:454764"
misc_feature 9025..9069
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438017"
misc_feature order(9250..9300,9304..9321)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438017"
misc_feature order(9400..9411,9415..9444)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438017"
CDS join(1..3339,3339..4100)
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="Truncated version of polyprotein that is predicted
to be produced from ribosomal frameshifting."
/citation=[1]
/codon_start=1
/product="truncated polyprotein"
/protein_id="YP_009268614.1"
/db_xref="GeneID:28218218"
/translation="MHRLRDLLPDKGKKNRSPAVRRQGGIAKGVDRKIIPSSGSKERK
VGTERKAKRVVARMKPNWLSFGTGNQRSMWRQIFSVDLMEGLLLFIALMSNLYERVQR
DIADLKRRVTRLEKERSHPRKVPIMLLCGLIVVTGLSIELALDENGDVEMWADNQNIT
NYAHLTKVPADVCIVGMVVTKHCPKVEKMTDLSDIDCGSTWVEFILTYTRCMILERET
RQKVDDKSPLTRFKEDLSTLETEAFQMLKRHAFSTILALITLAIVLKWPIWVVVLLGF
LAWNVVKGEFVEPFLVLKHDHNTMLTTRLYPGEIAHVATPTGLLDIRVGNAGIFGSQL
FRELLRDCKVNASYSTDICPGGSQLDMEAIQGPGRVCFTAPYNRGWGTGCFKWGIGAV
ATCVELNCSSSMGVHLLTNSAIVANVSVGFHSTNDTKMLVPDAPTTLKFGKLGTVTMN
CRLGNDRIATSYYHVTDGLSTGLFLKAQIDAWSGPFRMAGLTGGFDKIVKWGQVTPNE
IKVKRISEMELDWDNAITTHDGFVNTYFWCQIAVNKLVVGTFASCKSEAQTVFSQSPW
GFEGIAEITLKEAQKSICSLPLSCVGCSLLSTKVVFLETTTKAAVHVGCGNGTSVLTV
GTTPVSIDCVVTPLSQVWRLVSHVTGRYTKLGFGGVGGVFHDLLQACGWVFTWDSWKV
ILILGGLVVLFIVFDRKIVLVVMIASAVVYVKADVGCGIDFDRKTYTCGRGLFIWKGI
GTYPTSDHSVEFESYDFLAAYLKEQFKAERKVCIVCEDLVQCEAARKAASAIYRDLGH
PFVYVNVSSSYGKSFAEVPKRVHTVSVGVDVVEMAMMVSNNKPSGPFGELPTSVVSWS
TAPETEDHLVLRVVTSSTPYRQVCGKAIGFQYDFVGFRRAVYGSNVQLKISKKVSIEC
PTYLAGMAVKNDRTVFTDGMFWMSSRKDNDSYAIDELEMEQSHRCVWPNQYTPDMVQD
PRDNDLFVPPEWGGPRSKANHIPGYKMQISFPWDKAPIKLVDGSVPGTIVTQISKCEG
RGIPVMVDPAVNPNWCCKSCTRIFHFEVDGKLYYPMEIRPDVKEAKNKRSPVIEEPLG
DDTAETVTDWLSKTYRVPSTNAEDFSEPQIDFDEGKRNGGGSGKPSLFSVGYASRDSH
METQILGSFSLVLLVVSTVWCAHRLWLRRDLYMDEYSSDQPWFSPDVQPDYTSVGCTA
TPELKHVSVGTDTEIAISDILGWTDATAYHANAASGHLRALINVRMGYRSVLISYCYA
ELVHRGRPYSPEIDRVLSAIRMEDGTVHRMRFLVDILDEEMDDYRSSVTERGRVEVWI
SSLVFVHPDSFIYRCRRCGSHRIGLRWISGCVCGHRSLLNSRNQDD"
mat_peptide join(2146..3339,3339..4097)
/gene="POLY"
/locus_tag="BBA73_gp1"
/product="NS1-NS2AN-FIFO protein"
/protein_id="YP_009268629.1"
misc_feature 2152..3222
/gene="POLY"
/locus_tag="BBA73_gp1"
/note="Flavivirus non-structural Protein NS1; Region:
Flavi_NS1; pfam00948"
/db_xref="CDD:279316"
ORIGIN
1 atgcacaggt tgagagacct tcttcccgac aaggggaaga aaaatcgtag tcctgccgtt
61 aggcggcagg gcgggatcgc gaagggggtt gatcggaaga tcatcccttc ttcgggatca
121 aaagaaagga aagtcggaac agagagaaag gcgaagagag ttgtggctag aatgaaacct
181 aattggcttt cattcggaac tggaaatcaa cgatcaatgt ggcggcagat tttctccgtc
241 gatttgatgg aggggctact gctgtttatc gctctaatgt cgaaccttta tgagcgagtg
301 cagcgagaca ttgcggactt gaagagacgc gtgacaaggc tggaaaaaga gcgctctcac
361 cccagaaagg taccaatcat gctcctttgt gggctgattg tcgtgaccgg actatcgatt
421 gaactggcac ttgacgaaaa tggtgacgtg gaaatgtggg ctgataatca gaacataacg
481 aattacgccc atctgacaaa ggtaccggct gacgtttgca ttgttggaat ggttgtcacg
541 aaacattgcc ccaaagttga gaagatgacg gatttatctg acattgattg tggttcaaca
601 tgggtagaat tcattctgac gtacacgcgg tgcatgatct tggagagaga gacccgtcaa
661 aaggtagatg ataaatcccc actcaccagg tttaaagagg atctgtcaac cctcgaaacg
721 gaagcgttcc aaatgctcaa gaggcatgca ttttcaacca tcctagcgtt gataacattg
781 gcaatcgttt tgaagtggcc aatttgggtg gtcgttctgt tgggattctt agcctggaac
841 gttgttaaag gggagtttgt tgaacctttt cttgttctca agcatgacca caacaccatg
901 ttaacaacac gtctataccc cggagaaatc gctcatgtcg ctacgccaac aggcttgctt
961 gacattcgcg ttggaaacgc tggaatcttt ggaagtcaac tttttcgtga gctactccgc
1021 gattgtaagg ttaatgcatc gtactcaact gacatctgcc caggcggatc tcagttggat
1081 atggaagcca ttcaaggacc gggaagggtc tgttttacag ctccgtacaa tcgtggatgg
1141 ggaacaggct gtttcaaatg gggaattgga gctgttgcta cttgcgtaga gttgaactgt
1201 tctagtagca tgggtgtgca tttgctcacc aattcggcta tcgtggcgaa tgtgtcagtt
1261 ggatttcatt ctacgaatga cacaaagatg cttgttcctg atgctcccac aaccctcaag
1321 tttgggaaat tgggaactgt aacgatgaat tgccggcttg gtaatgacag gattgccact
1381 agttattacc atgttactga tggattgtca acaggcttgt tcttgaaagc acaaattgac
1441 gcttggtcag gcccattccg catggcggga ctgactggag gctttgataa gatagtgaaa
1501 tggggacaag tgactccaaa tgaaatcaag gtcaagcgga tctcggaaat ggaactagac
1561 tgggacaacg cgataacaac acatgatggg tttgtcaaca cgtacttttg gtgtcaaata
1621 gcggtgaaca agctagttgt gggcacattt gcctcgtgta aaagtgaagc gcagacagtt
1681 ttctcccaat ccccttgggg ttttgaagga atagctgaga taacgctaaa ggaggcccaa
1741 aagagcattt gttcattgcc tttatcttgt gtgggttgta gcttattgtc caccaaagtc
1801 gtgttccttg aaactacaac gaaagccgct gttcacgtcg gctgtgggaa tggaacttct
1861 gtcttgacag ttggaactac tcccgtgagc atcgattgtg tggtaacgcc tctgtcacag
1921 gtatggagac tcgtgtcgca cgtcaccgga aggtacacta aacttgggtt tggtggagtc
1981 ggtggagtgt ttcatgacct ccttcaggct tgtggatggg tgttcacatg ggactcatgg
2041 aaagtaattc tgattctcgg aggtttggtt gtattgttta tcgtgttcga tcggaaaatt
2101 gttctagttg tcatgatcgc cagcgccgtc gtctatgtga aggctgatgt gggatgtgga
2161 atcgattttg accgcaagac ctatacctgt ggacgtggat tgttcatttg gaaaggcatc
2221 ggaacttatc caacttcgga tcattcagtt gaattcgagt cgtatgattt cttggctgcc
2281 tacctgaagg aacagtttaa ggctgaacgg aaagtttgca tcgtatgcga agacttggtg
2341 caatgtgaag cggcgcggaa agccgccagc gcgatttacc gggaccttgg acatccattt
2401 gtgtacgtta acgtgtcatc atcatatggg aaatcgtttg ctgaagtccc aaagcgagtg
2461 catactgtca gtgttggagt tgacgttgtg gaaatggcca tgatggtatc taacaacaaa
2521 ccatctggac catttgggga attaccaaca agcgttgtct cttggtcgac tgctcctgaa
2581 actgaagatc atttagtttt aagggttgta acatcgtcca ctccatacag acaagtttgt
2641 gggaaggcaa tcggattcca gtatgacttc gtcggattta gaagagccgt gtatgggtca
2701 aatgtgcaat taaagatctc gaaaaaggtc tcaatagaat gccccacgta tttagctgga
2761 atggctgtca agaacgatcg gactgtgttt actgatggaa tgttttggat gtcatcaagg
2821 aaagataacg actcatatgc cattgacgaa ttggagatgg aacaatcgca taggtgtgta
2881 tggcccaacc aatacacccc tgacatggtt caagatccac gtgataatga tctgttcgtt
2941 cccccggaat ggggagggcc tcgatcaaag gccaaccaca tccccggata caaaatgcag
3001 ataagctttc catgggataa ggctccgata aagctagttg atggaagtgt gcccggaact
3061 atagtaaccc agataagtaa gtgtgaaggc cgtggaatac ctgtcatggt ggatccagcg
3121 gtgaatccta actggtgttg caaatcttgc accagaatct tccactttga agtcgatggg
3181 aagttgtatt atccgatgga aatacgtcca gatgttaaag aagcgaaaaa caagaggagt
3241 ccggtgattg aagaaccatt aggagacgac acggctgaga cggttacgga ctggctaagt
3301 aagacatata gagttccatc caccaatgct gaggattttc tgaaccccaa attgattttg
3361 acgaaggcaa acgcaatggt gggggaagtg gtaaaccttc tttgtttagc gttggctatg
3421 caagtcgtga ctcacacatg gaaacacaaa tccttggctc gttttcactt gtgctgcttg
3481 ttgtttctac tgtttggtgt gcccaccgtc tttggcttcg tagggatcta tacatggatg
3541 aatattcttc cgatcagcca tggttcagcc cggatgtgca acctgactat acatctgtgg
3601 gctgtactgc aacaccggag ctcaagcatg tttctgtggg gacagacact gagatcgcaa
3661 tttcagacat ccttggctgg acagatgcta ctgcttacca tgcaaatgct gcatcaggcc
3721 atctacgcgc actcatcaat gttaggatgg ggtatcgaag tgttcttatc agttattgtt
3781 atgcagaact tgtacaccgt ggtcgaccat attcacccga gattgatcgc gtattgtctg
3841 ctattcggat ggaggacggg actgtgcata ggatgcggtt tcttgttgac attcttgatg
3901 aagagatgga tgactatcgt agcagcgtca ccgagcgcgg gcgggtggag gtctggatat
3961 cgagccttgt gttcgtccac cctgactctt ttatttaccg ctgtcggcgt tgcgggagtc
4021 atcgcatcgg actacggtgg atatccgggt gcgtctgcgg ccatcgcagc ctgcttaata
4081 gcaggaatca agatgactga ctttcttgcc acgagattat cactggagtt tgtctcgact
4141 ggacaatttc ccgaagggac aaccatcgaa aaggagaaag aaacctatga cggaacgttt
4201 cgagcctcat tcacagtgga agggatcaag ctattggacc acacagaacc agtacctttg
4261 gtgtttgcga tttcgtatgt ggcccttgga gctgtgacgt gcaaaattca tccaggactt
4321 ggagtgattt atgctgtcgc aatggttgcc acaaacttac catctttgct acaagtttac
4381 gtgttgtcaa tctgcaccaa tgcctttcgg tcggatgaac tgattggaac tgccattgcc
4441 agtgttgagc cagagttgtc aaaagatttt ggcagtatac cagatggcat atatcgtgtc
4501 aacaaccatg gcattgcttt caagagtcat cgaggagtcg gtattgtgaa aaatggagtt
4561 ttccatacat tgatgcacat cacgatgaat gaacccgtta cttggcagaa caaattggtc
4621 gggccgtaca tgggcaattc tctgaaggat tatctgtgtt acggtggaaa ctggcagttg
4681 ccgtcgtttg accctactga tgaggttgga atcatggtgt gcaagagtga caggtcggta
4741 gagtacaaga gacatgaagt tggcaagatt gatgttgatg gagttccaca tatgtatttc
4801 acgaaggact atggacgagg aacatcagga tcgccaattt ttgtcaacgg agaaccagtt
4861 gcactgtatg gatttggatt ttttctctac aacacatacc gatccctagt tgttcctgtt
4921 ccacgagctg acatagaagc tggagagagt gctgaggggt tagtcgaaga aaatagctca
4981 atagtgaaca agtttttcgt tgactggcac cctgggaaag gtaaaacgcg gaaggtgatt
5041 gtcaaacatg ttcttgaggc aattaaggac tcgcgaagga ttttggttct agcaccaaca
5101 agagttgtca tggcggaaat aatgaaagca ctagaggaga gtacccacaa ggcgatctcg
5161 aagagcatgt cacatactag ccataatgga gtcacagttg cttgtcatgc aactttcact
5221 gacttcattc tagctcatgg tttgtcaaga ttcaaagctc acgaggtcat catggatgag
5281 tgtcactttc tcgacccacg atcaattgca gctagaggca ttcttgagca cttggcccaa
5341 aaacgcggag taaaagtagt tttcatgagt gcaacgattc ctggaagaga gccatcgatt
5401 ggttcgaact atgagatagc tgaacaagcc ctccagttcc cacgggaggt cacgtcaaag
5461 tggattggag atgtgtcgga aggaaaaacc gttgtgtttg tcccatccca taaggttgga
5521 gataaactag cgttgggcag tccacattca atttcattgc accggaacaa ttttaacact
5581 aattattcga tcgcaaggag tgatgacatc aagtacgtgt acacaactga catctcggag
5641 atgggggcaa atttcaatgc cacgactgta gttgactttc gtgtagccat aaaaccgaag
5701 attctaaatg aatgtgaagt tactttggct cccaccccaa tcactaggtc gtcgatggtc
5761 cagagaagag gaagagttgg ccggcaacac cctgggaaat acatctaccc agccaataaa
5821 ggaaccgaag aggcggcaaa tgatttagct tgttggacgg aagcccagat gatcttggat
5881 caattggatt tgaccatgat ggctgaggaa gccccctatt caaatcatcc aggagcgtac
5941 aagctagttg gaaagagctt tcaggttttt aagaaattat tggaagacaa agatgatatt
6001 cccatatggt tgagttggaa gtgggctgat aatactgagc accagtatgc tgccctcttt
6061 gaaggagaaa ggctggaaaa tgtaccaaga atggtcaaca cccgggagta tgcatccatt
6121 gaatacaaac ccaaatttgt tgatgccaga ttcgaacggc taagctggga gcaaaggaaa
6181 ttagccatcc agttctatat gggaacccgg agttttctat ctgttggtct tttctacaaa
6241 gttctgagtc agatcattca agctggtgta gtgaacacgg cttggaaaaa gattggcgac
6301 gtcggcttgg tttttatgga aggtggggat ccgcattcga aggatgagtc aatagttgct
6361 tggacgatct tgattggcgg gatgctttgt gttattgtct tgatgatatg tgtgtgggga
6421 cttcgtattg ttatgcgaat ggtctttgga tcgcgcgaaa aacactctag tgttccaaca
6481 ttgttagccg attcccaacc gtacctagtg tgcttggtcc ctgtcgctac tcatctcgca
6541 ggtgtcccag ttccagtgac catcgtcgtc tttgtgatat tgtttttgac atttccctta
6601 atatatcgaa gtgccggaca aagaagttat gtcgacatag atctggtaaa atggattctc
6661 gtgggtggag gtttcataat tggggtcatt tgttgggaat tgagactgct cccaaatatc
6721 acctctgatt tgcaatcaat ttcgcatggc agaactagaa ctgattccag tggatcgcgg
6781 caggaaagtg actgggggtt ttcatggatg gatttcgctc agccagtgcc aacaagtgtt
6841 gaattaacat ctgtcgtcat aacaaccttc acgatggcat tattcctgaa ccaaattgtt
6901 ggatggtcat atgaggcgga ttggttgaaa tcgtacttcg accacaaagg tgtaggtcat
6961 atcatgggtg gttttcgtct ggacacaatc tcgtggggct cagccctcag ctcacttttg
7021 gggactttgt cttatgcttc ttggggagct atcataattg ggatgggtgg agcaacgatc
7081 tatttctttt ttatggtaaa gatgttgaag tggaatttca caggaggctc aaccattgga
7141 ctagagaaca acaccatgag acaggatcga gaaacaggcc ttaatagaag accattccat
7201 gacaatagga gatcattatt gtatggagtt gtcatctttg aatgcgctct ctgggtattc
7261 tgctttatga gtgtctttga cttggttatt gcctcgtgtg tttcaacgta ctgtgtatgg
7321 atcattttca atgcctcgag tgagcaccat aggaatgttg accttgggtc ggtttgtagt
7381 ttcgttgggt tgttgtacgc cacttgtcca tcacaaaagt gtgtgcaggt cctcatcaga
7441 tttgcattgt ctagattgaa cataacgaca agatcactgg agaaaagtgc tactggcggt
7501 ttggggcaca gatggaagaa gatacttaat gcaatgtccc aggacgattt caactcgtac
7561 aggttgtacg gagtcgacga aacggataaa ggtgactacg tctcgagagg aggtctgaaa
7621 cttagggagc tgactttgaa gtatggatgg aaaccggaag ggatttgtgt cgacttaggg
7681 tgtggaagag gaggctggtc tcagcacttg gccatggatc caagagtcac gagaatcgaa
7741 gcatacacct tgggtgggtc aacgcgtgaa aacccccaac ctgtaaagac actaggccac
7801 aatttgatta gattcaaaac aggtgtaaat gtgtacaaca tggttccaac atttgcaaac
7861 accattgttt gtgatattgg tgagagcgac ccgaagaccg aagtggaagc ctccaggacc
7921 atccgtgtat tgagcacctt ggggacttgg ttagagaaga acccaaatgc ggaattcgtt
7981 tgtaaggtgt tgtgcccata tcctgttgaa gttctacggc ttttggaaac cttccagcac
8041 aagtatggtg gcaggattgt acgttcaacg tttagcagga actcctcagc cgaaatgtat
8101 tacatctctg gaggacgtaa taacattgtc aaagttgtgt tcacaacact gcatgcgcta
8161 atcgctagac ttcgaactaa accggacaag atcgtcaagg agccgatccg gttgccagtt
8221 ggcaccagga gtgacccggg gcacaaagtt aaggacatga accctaaaat gattgcaacg
8281 agagttgaga agctgagaaa tgagcacaag gacacgtggt tcattgatag caatcatcca
8341 taccagtcct tccggtatgt tggttcttac gttaccgatg acatcaaccc tggcggacag
8401 accgtaaatc ccttaatgcg aaagatgatg tggccatggg aattggttgg aggagtggtc
8461 aactttatga tgacggatgt gtcaacatac gctcagcaga aagttttgag agagaaagtg
8521 gacacgctgt cacctgaacc accaaagaac atcaagacta tcaaccgact catcactgaa
8581 ttccagatcc gaagctatac aaaacgagga ttgcgtcccc gcattctcac catggagcaa
8641 tacgcggaca atgtgaagag ttcagcggca attggatctt ggagttctga tgttccatgg
8701 aataatgtca gagctgccct cagcgatgcc aattttcatg cattggtaga tgaggaacgt
8761 cgattgcatt tggcaggtga ttgcaggatg tgtgtgtata acacgatggg aaaaaaggaa
8821 aagaaaccag catcaatggg agtggccaaa ggctcgagga caatctggta tatgtggcta
8881 ggatcaaggt tcttggaata tgaagctctt gggttcttga atgaggacca ttgggtttcc
8941 agggagaact tggcatgtgg tgtaggaggt gttggagtta actattttgg ctattatctg
9001 caagaaatat ccaagaaagg gaagttcttc attgccgacg acattgctgg ctgggacacc
9061 aggatcaatg aaagtgatct ggctgatgag gaacatctca tcatgtcgat gatttcagac
9121 acaagacatc gggccttagc cacggcggtt ttcaagtttg cttaccagaa catagttgct
9181 cttttcccta gaaatcaccc cgggttcgga agtgggactg ttatggatgt cgtcgctaga
9241 acagatcaac gaggatcagg acaagttgtg acctacgcct taaacaccat caccaatgcc
9301 aagattcagc ttgggaggat gattgaggct gaaggactgc ttggggcccc ggaatctgtc
9361 ataacaaagt ggcttgatga caatggcgaa gacagactat cagccatggt tgtagctgga
9421 gatgatgtgg ttgtggcgac aaataatgac aaattttctc atagtttgga ttacttgaat
9481 ctcaatggga aaatcaggaa ggacatcgac ccgtccctcc catcacgagt gcagaccaac
9541 tgggagacag tcgagttttg ttcccatcat taccacccaa tgactcttcg ggatggccgg
9601 cgtatcattg tcccctgccg tgatcaaaat gaggtcatcg ggcgaggtag gattcagaag
9661 ggtggtctgg ttaccattgc ggatagcgcg tgcctggcaa aagcctatgg tcagatgtgg
9721 gccttgtact tcttccaccg tcgagaccta cgaatggctt tcatggcaat aacatcaagt
9781 gtccccgtgg actggtttcc agaagggagg acctcatggt cgattcatca gaataaggag
9841 tggatgacaa cggaagacat gttgagagtg tggaacacgg tttggatcca tgacaacaac
9901 tggatggagg ataaaactga ggtgactgcc tggaaagaca taccctatct cccaaaatcg
9961 atcgacatca agtgtggtag ccaaatagga agtaaggatc gagccacttg gtcacgtgaa
10021 ttgccctcaa cggtcatggc tattagaaga attctagacc atgagaccag gatggaaaat
10081 gtttacaagg atttcctgag tggcatggga cgatttagag aagaaaatga tccaatggcg
10141 gccggagttc ggttttaa
//
