LOCUS NC_040835 7188 bp RNA linear VRL 12-SEP-2019
DEFINITION Rana hepevirus strain agile forg/RD6/2015/HUN, complete genome.
ACCESSION NC_040835
VERSION NC_040835.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Rana hepevirus
ORGANISM Rana hepevirus
Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
Hepelivirales; Hepeviridae.
REFERENCE 1 (bases 1 to 7188)
AUTHORS Reuter,G., Boros,A., Toth,Z., Kapusinszky,B., Delwart,E. and
Pankovics,P.
TITLE Detection of a novel RNA virus with hepatitis E virus-like
non-structural genome organization in amphibian, agile frog (Rana
dalmatina) tadpoles
JOURNAL Infect. Genet. Evol. 65, 112-116 (2018)
PUBMED 30053640
REMARK Publication Status: Available-Online prior to print
REFERENCE 2 (bases 1 to 7188)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (12-FEB-2019) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 7188)
AUTHORS Reuter,G. and Pankovics,P.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2018) Regional Laboratory of Virology, ANTSZ
Baranya County Institute of State Public Health Sevice, Szabadsag
u. 7., Pecs, Baranya H-7623, Hungary
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to MH330682.
##Assembly-Data-START##
Assembly Method :: GeneDoc v. v2.7
Sequencing Technology :: Illumina; Sanger dideoxy sequencing
##Assembly-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..7188
/organism="Rana hepevirus"
/mol_type="genomic RNA"
/strain="agile forg/RD6/2015/HUN"
/isolation_source="feces"
/host="Rana dalmatina"
/db_xref="taxon:2185281"
/country="Hungary"
/collection_date="Jun-2015"
gene 49..4788
/locus_tag="EXL61_gp1"
/db_xref="GeneID:41702133"
CDS 49..4788
/locus_tag="EXL61_gp1"
/note="ORF1"
/codon_start=1
/product="nonstructural protein"
/protein_id="YP_009553650.1"
/db_xref="GeneID:41702133"
/translation="MEFSFGSLSKPQQTQLLDALLAKQCSSQIQERLRRAPKIVYNLT
KEQKAYVQDAYGGDVVFTDSVPNTHPVLRSLHSLAEHTINSKIVSKHLDIGADLRACD
RRKHCSHACLKVDNARDQNRYLRATIEKQIKTNHCTQGAESCSFICDVGVSVHSAYDI
SSRQWVEIFDQHQLKIVYCWLHLPREMLHTNRVSLPEVGADVIISGSRAQFLPHDASL
GYEHNLDEWLKYIQHTGISNGVMNLTFQVEYWQGTQLHFTITRTTARGIMTRLTEPKV
GNFVRVPRLGCFKAKPPVLCPQQKIDATVAWGMKRQDGNFTYTNLATYAGAIKSSFKI
GNNFISMPWHISSADLHDVTITCYVYVCVLRYLRTQTLSKALERLRSEQSRGWFEKMW
KSITESFVADKTWCYKIKMYNNDLHLLSLLEEESKSIRLHCDSSCWKGTQYIKVDTPL
RASTKATAGSVPIASPVLPITQNSPPHGLTSDFDDYDSDSCSSLPAPPSDLDDFSLTK
PAPDVDPYVNTTGSLRTLSSLNLSVSGKDARFQRRNSLPNLHTNNAELHSSVSSTRPL
GLRPVETYSNLKDLDFKVATDHLSDASSIGGLSYVSTDSKIKKRKRPSKKQRAERRVE
NYAHDCKGQMRIRKGDYRTAWETGRYDCIVNASNCQMVLGTGIAAVVNDLFPGHQAKM
TAHGPISEKQAVRIEQVIHAVAPKNANKDQMVEVFDAVATQIKHGEHVLCCLLGAGVF
TNDPTASKKVAWAAACLSTLSCCHVTFVDLDPPHICKMQNIERYYDNWTTHMSNEVFN
AARRTDADGEPDVFAPLNAELEEFVVKPGPCVGKVILHEGLPGAGKSHGLKQQLKDDC
RGVWVLVPTKKQKEEWEVTGAKVYTSTKGILEFLKTQEKPTTVIMDEWMMIHPWVIAT
VLASCNVTLCGDPTQIGFIDFGGLGLDYDHKIWRKCGVTFQHSVTRRCPRDVVDQIFK
HMYPNATTTSKVERSVTFARKVPSAGQVITYTQRAKKKYKGAITVHESQGLTFDNVAL
VVTDDAAHLINTSSAHNTVAFTRHTQSLVVVEDSTLFFSQAWDTPNLLADMFGFPHPL
REGHMPPESYLTFDLMPTNTSKNSMGVQQSDLVTVYRPTEGVTMRAVTSLNQPITGVV
TGNEDAVVPVAAGAFINLSPDCEMHVRVSANNDAAAAFAAGNNRYTGFQLRPSVTECV
DRGNRLMEALSVFVDLRRFKDSFDEDKMCPATAAAFSDAYKKQGTRFAEAIDPDNIPT
ELTAVKSFLKNQCKYAKDALHKEKVGQGISAWSKLANVLLCGYAREFERCLKDCMHQG
VVYANGMSDDEFDRSVRVFFCSKGVNFQNDFTEFDSTQGPVTKCFEKLMMRECGVPSA
VIDLYSELRSQWRMVDQGKVCVTNGEMRSSGEPFTLVGNTLVTMAVTAEMLQINDLSF
AAFKGDDSIVNGDVVYKANDDYIFQQYGMKVKPDVTEPPEFVSYILSPEGLHPDLVRI
AGKAFGKTIDPSRIPSLRVALNDQVKRMNDHQCYERSLELNMRRHALTKAEAMILYES
CENFIRGDRMELTPQMHLKLTSCGGLV"
misc_feature 169..1038
/locus_tag="EXL61_gp1"
/note="Viral methyltransferase; Region: Vmethyltransf;
pfam01660"
/db_xref="CDD:396298"
misc_feature 1999..2343
/locus_tag="EXL61_gp1"
/note="macrodomain superfamily; Region: Macro_SF; cl00019"
/db_xref="CDD:444655"
misc_feature order(2014..2016,2050..2052,2056..2058,2158..2160,
2245..2268)
/locus_tag="EXL61_gp1"
/note="putative ADP-ribose binding site [chemical
binding]; other site"
/db_xref="CDD:394871"
misc_feature 2560..3231
/locus_tag="EXL61_gp1"
/note="Viral (Superfamily 1) RNA helicase; Region:
Viral_helicase1; pfam01443"
/db_xref="CDD:366646"
misc_feature 3856..4395
/locus_tag="EXL61_gp1"
/note="conserved catalytic core domain of RNA-dependent
RNA polymerase (RdRp) from the positive-sense
single-stranded RNA [(+)ssRNA] viruses and closely related
viruses; Region: ps-ssRNAv_RdRp-like; cl40470"
/db_xref="CDD:454764"
misc_feature 4081..4125
/locus_tag="EXL61_gp1"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438017"
misc_feature order(4264..4314,4318..4335)
/locus_tag="EXL61_gp1"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438017"
misc_feature order(4351..4362,4366..4395)
/locus_tag="EXL61_gp1"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438017"
gene 4951..6345
/locus_tag="EXL61_gp2"
/db_xref="GeneID:41702134"
CDS 4951..6345
/locus_tag="EXL61_gp2"
/codon_start=1
/product="ORF2"
/protein_id="YP_009553651.1"
/db_xref="GeneID:41702134"
/translation="MAERQRPQATPPAQPQSELRKCKSCSALVKRGNFPAHNSKCPGR
ILVKKSQPLPRPAVVMGGKEKKKSSASRSVTPLEVRMMRRLSTWRPGEIIMNPMFQYQ
NVSNATWSKTWTNTTPVNMDLLVLTLGGYATPNIVCQSASDSPNTLSFLNAPLPLLGA
PPTVSTGLSADWNRDYINLGGEFTTGVPTADVEKWVPLSYKLIVNVSAPVTTQTGYMY
AYNREINPRIAATTYNVAPGTAYTSASTPFNGNFYGLQIPGVKSVHQQMATQVGNVHP
ITTGCTLVMDTYPDKLEPDSLITANTPTGAAVTAAITAGSAANLAGSGLGASPAGTYP
TVPATVFRPQLVGANQQTQLFINDTGSTTDLTIPGTTIAPSTAQQISSGLSYYFALNQ
LGMKKAQIVLLTGLAPGTSVRVSLNRLDTVVCQPGTQGTSWQVKYTGPKVDPDDIFPK
DTIRFMSGSGMSLK"
gene 6623..7066
/locus_tag="EXL61_gp3"
/db_xref="GeneID:41702135"
CDS 6623..7066
/locus_tag="EXL61_gp3"
/codon_start=1
/product="ORF3"
/protein_id="YP_009553652.1"
/db_xref="GeneID:41702135"
/translation="MERQCFSLCQLVELNELREVLQLNQHSTFTAQMALLRNLKIAAN
LDDNDWFEYVEEHSVHADNRPLRFDMFDTADDVLLQIHPAWAWPGTVQIPDYFSKNHL
CPPDQCLRCFFRKAFLNSSLWLSWWDYDQSPYPSDHSEAESSDSD"
ORIGIN
1 gctgagcctt cttttatgtt gtcagtctca agcctctatt gaaataatat ggagttttct
61 tttgggtccc tctccaagcc tcagcagacg cagctgctgg atgcgttgtt ggctaaacaa
121 tgttccagtc agattcaaga gcgcttgcgt agagccccta aaattgtgta caatttaacc
181 aaggagcaga aagcttatgt gcaggatgcc tatgggggcg atgttgtctt taccgactcc
241 gtccccaata cgcaccctgt ccttcgcagt ttacactctc tggctgagca cacaatcaat
301 tctaagattg tttctaaaca cctcgatatt ggagctgatc tccgggcttg tgatcgacga
361 aaacactgca gtcatgcatg tttgaaagtt gacaatgccc gagaccagaa ccggtatttg
421 agagctacta ttgaaaaaca aattaaaact aatcactgca ctcaaggagc cgaatcctgc
481 agtttcatct gcgatgtcgg tgtttccgtg catagtgcat atgacatctc cagccgtcag
541 tgggtggaga tatttgacca gcatcaattg aagatcgttt actgctggct tcatctgcct
601 cgtgaaatgc tccataccaa ccgagtttca cttcccgagg tcggggcaga tgttattata
661 tcagggagtc gtgcccaatt tttacctcac gacgctagcc tcgggtatga acataacttg
721 gacgagtggt tgaaatatat ccaacacacc ggtatttcaa acggtgttat gaatttaact
781 tttcaggtgg agtattggca gggcacgcaa ctccacttca cgattacccg taccaccgct
841 cgaggtataa tgactcgttt gacagagcca aaagtcggga attttgtgcg tgtccctcgt
901 ctgggttgct ttaaagccaa gccacctgtt ctctgtcccc aacaaaaaat agacgccact
961 gttgcgtggg gcatgaaacg tcaagatggg aatttcactt ataccaattt ggccacttat
1021 gccggggcta ttaaaagtag ttttaagata ggcaacaatt ttatatctat gccctggcat
1081 atatcctcgg ccgacctaca cgacgtcaca ataacttgtt atgtttacgt ctgtgtactg
1141 cgatatttgc ggacgcaaac gttgtctaag gcgcttgaac ggctccgctc agaacaatcg
1201 cgaggttggt ttgaaaaaat gtggaaatcc attactgaaa gctttgtggc agacaagact
1261 tggtgttata agatcaagat gtacaacaat gatttacatc tattgtcgtt gcttgaggaa
1321 gaatccaaga gcattaggtt acactgtgac agcagctgtt ggaaggggac acaatacata
1381 aaagttgaca cgccgttgcg tgcgagcaca aaggccactg ctggttctgt gcctattgct
1441 tccccggtgt tacctattac ccaaaattca ccgccacatg ggctaacatc agattttgac
1501 gattatgatt ctgattcgtg ttcgtctttg ccggccccac cctcggatct tgatgatttt
1561 tcgttgacaa aaccagcccc cgacgtagac ccgtatgtta acaccactgg gtcgttgaga
1621 actttgtcta gtttaaatct gtctgtgtcg ggcaaggatg cacgttttca gcggcgcaac
1681 tcgctcccaa atctacatac caataacgct gaattgcatt cttcagtaag ttcaacgcgc
1741 ccgttggggt tgcgcccagt tgaaacctat tccaatctga aagatttaga ttttaaagtg
1801 gccaccgatc atttatctga cgcctcttct attggtgggc tgtcttatgt ttccacggac
1861 agcaaaatta agaagcgcaa gcgcccctcc aaaaaacaga gagctgagag gcgtgttgaa
1921 aattatgccc atgactgcaa ggggcagatg cgcattagga aaggtgatta cagaacggcc
1981 tgggagaccg ggagatatga ttgtattgtg aatgcttcta attgtcagat ggtccttgga
2041 acgggtattg cagccgttgt aaacgacctg ttcccgggcc accaggcgaa gatgaccgca
2101 cacggaccga tttctgaaaa acaagctgtc cgtattgaac aggtcatcca tgcagtcgct
2161 cctaagaatg caaacaaaga tcaaatggtt gaggtttttg atgccgttgc gactcaaatt
2221 aagcacggcg agcatgtttt atgttgtctt ttgggggccg gagtattcac caatgatccc
2281 actgcgtcta agaaagttgc ttgggccgcg gcttgtttga gcaccttgag ctgttgtcat
2341 gtgacgtttg ttgatttgga cccacctcat atttgcaaga tgcaaaatat tgaacgttat
2401 tatgacaatt ggactactca tatgagtaat gaagtgttca atgcggcccg tcggacagac
2461 gcagacggcg aaccggatgt tttcgcgcct ttaaacgctg agttagagga gtttgttgtg
2521 aaacccggcc cttgtgttgg aaaggtgatt ttgcacgaag gtttacccgg tgccggtaaa
2581 agccacggtt tgaaacaaca acttaaggac gactgccgtg gcgtatgggt tttggttccg
2641 acaaagaagc agaaggagga gtgggaagta accggtgcca aagtctacac cagcacgaaa
2701 gggattttag agttcctcaa gacgcaggaa aaacccacca ctgtaattat ggacgagtgg
2761 atgatgatac acccttgggt gattgctact gtgttagcgt cctgcaatgt cacgctgtgc
2821 ggcgatccca cccaaattgg gtttattgat tttggcggcc tgggtttgga ttatgaccac
2881 aaaatttgga gaaagtgcgg cgttacgttt caacacagtg tgacgaggcg gtgtcctcgt
2941 gatgtggtgg atcaaatttt caagcacatg taccccaacg ccacaacaac tagtaaagtt
3001 gaacgatcag tgacgtttgc ccgcaaagta ccttctgcag gtcaagttat cacttacacg
3061 cagagggcaa aaaaaaaata taaaggggcc ataacagtgc atgaaagtca gggcctgacc
3121 tttgataatg tggcgttggt tgtcactgat gatgcggctc acctgatcaa cacttcttcg
3181 gcccacaaca ctgtagcttt cacccgccac acacaatctt tggttgtggt cgaagattcc
3241 acacttttct tttctcaagc ttgggatacc cccaacttgc tagctgatat gtttggcttt
3301 ccacatcctc tgcgtgaagg acatatgcca cctgaaagtt atttaacatt tgatttaatg
3361 ccgacaaata ccagcaagaa cagtatgggc gttcagcaat cagaccttgt aacggtttat
3421 agacctactg aaggggtcac aatgcgtgcc gtcaccagtc tgaatcaacc aatcaccggg
3481 gttgttacag gcaacgagga cgcggttgtg cctgtagcag ccggagcttt tattaacctc
3541 agccccgact gcgaaatgca tgtgcgtgtc agcgctaata atgatgcagc tgcggcattc
3601 gctgctggga acaaccgtta caccggtttc cagcttagac cttctgtgac cgagtgtgtt
3661 gatagaggca accgtttaat ggaagctttg agtgtttttg ttgacctacg cagattcaaa
3721 gattcatttg atgaggacaa aatgtgtccc gctactgcag cagcttttag cgatgcgtac
3781 aagaaacaag gcacccggtt cgcagaagct atagacccag acaacattcc gactgaattg
3841 actgctgtca agtcgtttct taagaatcag tgtaaatacg ccaaggatgc acttcataag
3901 gagaaagttg ggcaagggat aagtgcgtgg tctaagttgg caaatgttct cttgtgtggg
3961 tatgctcggg agttcgaacg atgtcttaag gattgcatgc atcagggtgt tgtttatgcc
4021 aatggtatgt cggacgatga atttgaccgc agcgtccgtg tatttttttg ctccaagggt
4081 gttaattttc agaatgattt tactgagttt gacagtactc aggggccggt gacaaaatgt
4141 tttgaaaaac tcatgatgag agaatgtggg gttcccagcg cagtcataga tctgtactct
4201 gagctgcgtt ctcaatggcg aatggttgac caagggaaag tgtgtgtgac taatggggag
4261 atgcgcagta gtggcgaacc ttttacgctg gttggcaata ctttggtcac gatggccgtc
4321 accgccgaaa tgttgcagat taacgatttg tcttttgcgg cattcaaggg tgacgacagt
4381 attgtgaacg gcgatgttgt gtacaaagca aacgatgatt acatattcca gcaatatggt
4441 atgaaggtta agcccgatgt tactgaaccg ccggagtttg tcagttacat tttgtccccg
4501 gaaggtttgc acccagattt ggttcgcatt gctggtaagg catttggaaa aacgatagat
4561 cccagccgaa ttccatctct acgcgtcgcc cttaacgatc aagttaaacg gatgaatgac
4621 catcagtgtt atgagcgaag tttggaattg aacatgcgca ggcatgcatt gacgaaggct
4681 gaagccatga tattgtatga gagttgtgaa aattttataa ggggtgacag aatggagcta
4741 acaccacaaa tgcatttgaa gctaacatcg tgtggtggat tggtgtgagt ccaataggta
4801 attgcgcaga aagaaagtac tcttttcctg tgcgtcacca atgaactata ctgttcccgc
4861 tcctatttta gagtgttctg aaaaccatct gaatattctg attgttctga ttgcacagat
4921 tttgcttgct gtattgtata agatttggct atggctgaaa ggcaaagacc ccaagcaact
4981 cctcccgccc aaccccaatc tgagttgcgc aagtgtaagt cttgttccgc ccttgtcaag
5041 cgaggcaatt tcccagccca caattctaaa tgtcctggac gaatcctcgt caagaaatct
5101 cagcctttgc cacgacccgc tgttgtgatg ggcggcaaag aaaagaagaa gtcatcggca
5161 tcgcgttccg taaccccttt ggaggtcaga atgatgcgac gcctatcgac ttggcgtcca
5221 ggggagatca ttatgaaccc catgttccaa taccagaatg tctctaatgc aacctggtcg
5281 aaaacctgga ccaacaccac tcctgtgaat atggatcttc ttgtgctgac ccttggaggt
5341 tacgccaccc ccaatattgt atgccagagt gcttctgaca gccctaacac tctctccttt
5401 ctgaatgcgc ctctgccgct tttgggtgca ccacccactg tttccacggg actcagcgcg
5461 gactggaata gagactacat caatctcggg ggagagttta caacaggagt ccccacagct
5521 gacgtggaaa aatgggtgcc actttcatac aagttaattg ttaatgtcag tgcgcccgtc
5581 accacgcaaa ccggttatat gtatgcctat aaccgggaaa tcaatcctcg gattgcggca
5641 accacctaca acgtggctcc tgggaccgct tacacatctg cttcaacccc ctttaatgga
5701 aatttttatg ggttgcagat tcccggggtg aaaagcgttc atcagcagat ggccactcag
5761 gtcggaaatg ttcaccctat aacgaccggg tgcacacttg tcatggatac ttaccctgac
5821 aagctggaac cagacagtct gatcactgct aacacgccca ctggggctgc agttaccgca
5881 gccattacag ctgggagcgc cgctaatttg gcaggcagcg gtctaggagc ttcacccgcg
5941 ggaacgtatc caacggttcc tgccactgtg ttccgtcctc aacttgtagg ggccaaccag
6001 cagacccagt tgttcattaa tgacacaggg tctaccacgg atttaactat tccaggcacc
6061 acaatcgccc cgtcaaccgc acagcagata agctcgggtt tgagctacta ctttgctctc
6121 aatcaacttg ggatgaagaa agctcagatc gtgcttctta caggtctggc tccgggcacc
6181 agcgttcgtg tgtctcttaa ccgtcttgac accgttgtgt gccaacctgg cactcaggga
6241 acttcatggc aggttaagta taccggccca aaagtggatc ccgatgatat tttccccaag
6301 gacacaatac gtttcatgtc cggaagcgga atgtctctga aatgaaaatt tgctggttca
6361 ggagaagaac taaatttgta tatttaaatt tattttgtat ttgaatgttt taaaatgttt
6421 gtaatgaagg aatatgtgga agaaggaaag ctcaattttc cttgcgcttg aagtacgtcg
6481 agacgtcgtt tgttcagatt tatggctgag accgtttatc ctttgggtgt cttgtgtcaa
6541 aaacttcgtt tggtgttgaa tttcaccgag gaacaattga caccggacgt tccttatttt
6601 aaatcggtct gcacaattcg acatggaacg acagtgtttc agtttgtgtc aactggtcga
6661 actaaacgag ctgcgcgaag ttctgcagct caatcagcac tcgacgttta cagctcaaat
6721 ggcacttttg cgcaatctta aaatagcagc taatttggac gacaatgatt ggtttgaata
6781 tgtcgaggaa cattccgtgc atgccgacaa cagaccttta cggtttgata tgtttgacac
6841 agccgatgat gtgctgttgc agatacatcc agcttgggcc tggcccggca cggttcagat
6901 ccctgattat ttcagtaaaa accatctttg ccctccggat cagtgtttgc gctgcttctt
6961 ccgcaaggca ttcttaaaca gctcgctctg gttgtcgtgg tgggactatg atcagtcccc
7021 ctacccctca gatcacagtg aagctgagag cagtgatagt gattgaacac tgctcagccg
7081 cgagtaacag cgggatactt tgaagatgtt acaacgtcag ttttcatgtt tgtttgctaa
7141 aacatgcttt ttaggttctg atttggcgaa tcagctcgtt aaccagcc
//
