LOCUS NC_055508 15270 bp RNA linear VRL 07-JUN-2021
DEFINITION Alston virus isolate Alstonville, complete genome.
ACCESSION NC_055508
VERSION NC_055508.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Alston virus
ORGANISM Alston virus
Viruses; Riboviria; Orthornavirae; Negarnaviricota;
Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae;
Rubulavirinae; Orthorubulavirus; Orthorubulavirus alstonvillense.
REFERENCE 1 (bases 1 to 15270)
AUTHORS Johnson,R.I., Tachedjian,M., Rowe,B., Clayton,B.A., Layton,R.,
Bergfeld,J., Wang,L.F. and Marsh,G.A.
TITLE Alston Virus, a Novel Paramyxovirus Isolated from Bats Causes Upper
Respiratory Tract Infection in Experimentally Challenged Ferrets
JOURNAL Viruses 10 (12), E675 (2018)
PUBMED 30487438
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 15270)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (01-JUN-2021) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 15270)
AUTHORS Johnson,R.I., Tachedjian,M. and Marsh,G.A.
TITLE Direct Submission
JOURNAL Submitted (20-SEP-2018) Health and Biosecurity, CSIRO Australian
Animal Health Laboratory, 5 Portarlington Rd, Geelong, Victoria
3219, Australia
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to MH972568.
##Assembly-Data-START##
Assembly Method :: CLC Genomics Workbench v. 8.5.1; SPAdes v.
2.5.1
Sequencing Technology :: Illumina; Sanger dideoxy sequencing
##Assembly-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..15270
/organism="Alston virus"
/mol_type="genomic RNA"
/isolate="Alstonville"
/isolation_source="urine"
/host="Pteropus sp."
/db_xref="taxon:2495433"
/country="Australia"
/collection_date="12-Jul-2011"
misc_feature 1..55
/note="leader sequence"
gene 56..1738
/gene="N"
/locus_tag="KM672_gp1"
/db_xref="GeneID:65102427"
CDS 158..1687
/gene="N"
/locus_tag="KM672_gp1"
/codon_start=1
/product="nucleocapsid"
/protein_id="YP_010087167.1"
/db_xref="GeneID:65102427"
/translation="MSSVLKAYERFTLTQELQDQSEEGTIPPTTLKPVIRVFILTSNN
PELRSKLLLFCLRIVLSNGARDSHRSGALLTMFSLPSAAMLNHVKLADQSPEADIERV
EIDGFEDDSFRLIPNARSGMGRGEINAYAALAEDLPDTLNHATPFVDPDVENTPWDEI
ETFLDMCYSVLMQAWIVTCKCMTAPDQPVASIEKRLQKYRQQGRINPRYLLQPEARRI
IQAIIRKGMVVRHFLTFELQLARAQSLVSNRYYAMVGDVGKYIENCGMGGFFLTLKYA
LGTRWPTLALAAFSGELTKVKSLMALYQTLGDQARYLALLESPHLMDFAAANYPLLYS
YAMGIGYVLDANMRNYAFSRSYMNKTYFQLGMETARKQQGAVDMRMAEDLGLTQAERA
EMANTLAKLTTANRGADARGGVNPFAAVAGPSQPQASAVSDTFESYMAADRMRQRYAE
AGAYDNEMPPLEEEEEDDTMGVSRTGPTLEQVAIDIQNNPPSGPTNNDDLNAALGDLD
I"
misc_feature 158..>1444
/gene="N"
/locus_tag="KM672_gp1"
/note="Paramyxovirus nucleocapsid protein; Region:
Paramyxo_ncap; pfam00973"
/db_xref="CDD:395775"
gene 1304..3098
/gene="P/V/W"
/locus_tag="KM672_gp2"
/db_xref="GeneID:65102421"
CDS 1856..3032
/gene="P/V/W"
/locus_tag="KM672_gp2"
/exception="RNA editing"
/note="insertion of two glycine residues into mRNA to
generate the P protein"
/codon_start=1
/product="phosphoprotein"
/protein_id="YP_010087168.1"
/db_xref="GeneID:65102421"
/translation="MDPTDLSFSSEEINKLIETGLNTVEYFTSQQVTGQSSLGKNSIP
PGVTGILTNAAEAKIQESIAPIKPGTGGGARPKKARPKIAIVPADDKTVPGKPIPNPL
LGLDSTPSTQSVLDLSGKTHPTGSYKGVTLAKFGKENLMTRFMEEPRESPIATSSPVD
FKRGAGVPVVTIEGNTLSDGWEMKSKSQSGAIHPAPQSQQQQGDLNALVTSVQSLALN
VSEILNTVRGMDTRLNQIESKVDRIMSSQSLLQTMKNDIIGLKAGMATLEGMITTVKI
MDPGVPSNVTVEDVRKTLSNHAVVVPESFNDSFLTQSEDVLSLDDLARPTATNVKKIV
RKVPPPKDLTGLKITLEQLAKDCISKPKAREEFLIKINQATSESQLHDLKKSIIRSAI
"
misc_feature 1868..2041
/gene="P/V/W"
/locus_tag="KM672_gp2"
/note="N-terminal region of Paramyxovirinae phosphoprotein
(P); Region: Soyouz_module; pfam14313"
/db_xref="CDD:316803"
misc_feature 2558..3019
/gene="P/V/W"
/locus_tag="KM672_gp2"
/note="C-terminal domain of Measles virus phosphoprotein
and related proteins; Region: MEV_P-protein-C_like;
cl03936"
/db_xref="CDD:413868"
CDS 1856..2524
/gene="P/V/W"
/locus_tag="KM672_gp2"
/codon_start=1
/product="V protein"
/protein_id="YP_010087169.1"
/db_xref="GeneID:65102421"
/translation="MDPTDLSFSSEEINKLIETGLNTVEYFTSQQVTGQSSLGKNSIP
PGVTGILTNAAEAKIQESIAPIKPGTGGGARPKKARPKIAIVPADDKTVPGKPIPNPL
LGLDSTPSTQSVLDLSGKTHPTGSYKGVTLAKFGKENLMTRFMEEPRESPIATSSPVD
FKRGRGTGGYHRREYSIGWVGDEVKVTEWCNPSCSPITAAARRFECTCNQCPVTCSEC
ERDT"
misc_feature 1868..2041
/gene="P/V/W"
/locus_tag="KM672_gp2"
/note="N-terminal region of Paramyxovirinae phosphoprotein
(P); Region: Soyouz_module; pfam14313"
/db_xref="CDD:316803"
misc_feature 2381..2515
/gene="P/V/W"
/locus_tag="KM672_gp2"
/note="Zinc-binding domain of Paramyxoviridae V protein;
Region: zf-Paramyx-P; pfam13008"
/db_xref="CDD:372427"
CDS 1856..2371
/gene="P/V/W"
/locus_tag="KM672_gp2"
/exception="RNA editing"
/note="insertion of one glycine residue into mRNA to
generate the W protein"
/codon_start=1
/product="W protein"
/protein_id="YP_010087170.1"
/db_xref="GeneID:65102421"
/translation="MDPTDLSFSSEEINKLIETGLNTVEYFTSQQVTGQSSLGKNSIP
PGVTGILTNAAEAKIQESIAPIKPGTGGGARPKKARPKIAIVPADDKTVPGKPIPNPL
LGLDSTPSTQSVLDLSGKTHPTGSYKGVTLAKFGKENLMTRFMEEPRESPIATSSPVD
FKRGQGYRWLP"
misc_feature 1868..2041
/gene="P/V/W"
/locus_tag="KM672_gp2"
/note="N-terminal region of Paramyxovirinae phosphoprotein
(P); Region: Soyouz_module; pfam14313"
/db_xref="CDD:316803"
misc_feature 2343..2346
/gene="P/V/W"
/locus_tag="KM672_gp2"
/note="mRNA editing site"
gene 3132..4509
/gene="M"
/locus_tag="KM672_gp3"
/db_xref="GeneID:65102422"
CDS 3165..4298
/gene="M"
/locus_tag="KM672_gp3"
/codon_start=1
/product="matrix protein"
/protein_id="YP_010087171.1"
/db_xref="GeneID:65102422"
/translation="MPSISIPADPTNPRQSIKAFPIIINSNNGERGRLVKQLRTTYLN
DLDAHEPLVTFVNTYGFIYEQDRGNTIIGEDQLGKRREAVTAAMVTFGCGPNLPSLGN
ILNQLNDFQVVVRKTSSKAEEIVFEVIKHPRIFRNHTLIQKGVVCVSAEKFVKSPGKI
QSGMDYVFIPTFLSVTYCPAAIKFQVPGPMLKMRSRYTQSLQLELMIRILCKPDSPLM
KVHTPDKDGRGCLVSVWLHVCNIFKSGNRNGSEWQEYWMRKCANMQLEVSIADMWGPT
IIIHARGHIPKSARLFFGKGGWSCHPLHEVVPSVTKTLWSVGCEITKAKAIIQESSIS
LLVETTDIISPKVKVSSKHKRFGKSNWGLFKKTKSLPNLTELE"
misc_feature 3183..4211
/gene="M"
/locus_tag="KM672_gp3"
/note="Viral matrix protein; Region: Matrix; pfam00661"
/db_xref="CDD:395535"
gene 4532..6248
/gene="F"
/locus_tag="KM672_gp4"
/db_xref="GeneID:65102423"
CDS 4560..6188
/gene="F"
/locus_tag="KM672_gp4"
/codon_start=1
/product="fusion protein"
/protein_id="YP_010087172.1"
/db_xref="GeneID:65102423"
/translation="MASLLVGLMLLGLTTGSTGLDPTALMQIGVIPTNVRQLMYYTEA
SSSYIVVKLMPTIDSPISGCNISSIASYNSTITKLLQPIGENLETIKSQLVPTRRRRR
FAGVVIGLAALGVATAAQVTAAIALVKANENTAAILNLKASIQKTNAAVADVVQATQS
LGTAVQAVQDHLNNVVSPAITAANCKAQDAIIGSILNMYLTELTTIFHNQITNPALTP
ITIQALRILLGSTLPTVVEKAFNTQISAAELLSSGLLTGQIVGLDLSYMQMVIKVDLP
TLTTQPATQIVDLATISAFINNQEVMAQLPTRIMVTGGLIQSYPASQCTITPSTVYCR
YNDAQVLSEDTVACLQGNLTRCTFSPVVGSFMTRFVLFDGIVYANCRSMLCTCMQPAS
VILQPNSSPITVIDMYKCVSLQLDNLRFTITQIANATYNSTIKLSASQILPIDPLDIS
QNLAAVNKSLNSALQNLAQSENYLSAITSANTTSILAIIALCIGILGLLLVILIAVVI
WKLVSIVSTERTRMDNYVYHNQAFQQSQLDLNKP"
misc_feature 4632..6023
/gene="F"
/locus_tag="KM672_gp4"
/note="Fusion glycoprotein F0; Region: Fusion_gly;
pfam00523"
/db_xref="CDD:395418"
gene 6254..6539
/gene="SH"
/locus_tag="KM672_gp5"
/db_xref="GeneID:65102424"
CDS 6333..6467
/gene="SH"
/locus_tag="KM672_gp5"
/codon_start=1
/product="short hydrophobic protein"
/protein_id="YP_010087173.1"
/db_xref="GeneID:65102424"
/translation="MLSDPESQNSNENTKKAGNFIICLLFIFFLLITFIVPAIRYFLS
"
gene 6541..8416
/gene="HN"
/locus_tag="KM672_gp6"
/db_xref="GeneID:65102425"
CDS 6608..8305
/gene="HN"
/locus_tag="KM672_gp6"
/codon_start=1
/product="attachment protein"
/protein_id="YP_010087174.1"
/db_xref="GeneID:65102425"
/translation="MGADEAPLKGTCRVLFRTTTLLLLCTLLALSVAVLYEVLITQQY
LKNNSGSSNLPSGLSGITDSLANILSVVNQIVYNSAVAIPLQLDTMESTLLTAIKSLQ
TSDKLEPNCSWGAALINDNRYIHGINQFYFSPTEGQNLTLGPLLNIPSFIPTATTPEG
CTRIPSFSLTKTHWCYTHNVILNGCLDHVSSNQFVSMGIIEPTSAGFPSFRTLKTLYL
SDGVNRKSCSISTIPGGCMMYCFVSTQSEKDDYLSAAPPEQRIIMMHYNDSIVERIIN
PPGVLDVWATLTPGTGSGVYYLGWVLFPVYGGVIRGTNLWNSQNNKYFISKMASTLCK
QDQATQIANAKNSYYSSWFGDRLIQAGILACPLQRDLTNECLVLPYSNDQVMMGAEGR
LYIYGDSLYYYQRGTSWWPMTMLYKLTLTFSNNQPSAITAQNIPTQQVPRPGTGGCSA
DNRCPGVCLTGVYADAWLLTDPAAVNSFGSQIIFTGSYLNSATQRINPTMYIANNTKI
LNSQQFGANGQGASYGHTTCFRDTGSVNVYCIYIIEVSSSLLGQFQIVPFVRQINLN"
misc_feature 7052..8290
/gene="HN"
/locus_tag="KM672_gp6"
/note="Hemagglutinin-neuraminidase (HN) of parainfluenza
virus 5, Newcastle disease virus, and related
paramyxoviridiae; Region: HN; cd15469"
/db_xref="CDD:271233"
misc_feature order(7064..7081,7151..7153,7187..7189,7214..7216,
7220..7222,7256..7258,7262..7264,8246..8257,8267..8269,
8273..8281)
/gene="HN"
/locus_tag="KM672_gp6"
/note="dimer interface [polypeptide binding]; other site"
/db_xref="CDD:271233"
misc_feature 7091..7267
/gene="HN"
/locus_tag="KM672_gp6"
/note="Sialidase propeller 1 [structural motif]; Region:
Sialidase propeller 1"
/db_xref="CDD:271233"
misc_feature order(7094..7096,7346..7348,7469..7471,7523..7525,
7661..7666,7775..7777,7820..7822,7826..7828,7982..7984,
8090..8092,8174..8176)
/gene="HN"
/locus_tag="KM672_gp6"
/note="receptor binding site [chemical binding]; other
site"
/db_xref="CDD:271233"
misc_feature 7274..7432
/gene="HN"
/locus_tag="KM672_gp6"
/note="Sialidase propeller 2 [structural motif]; Region:
Sialidase propeller 2"
/db_xref="CDD:271233"
misc_feature 7457..7762
/gene="HN"
/locus_tag="KM672_gp6"
/note="Sialidase propeller 3 [structural motif]; Region:
Sialidase propeller 3"
/db_xref="CDD:271233"
misc_feature 7766..7921
/gene="HN"
/locus_tag="KM672_gp6"
/note="Sialidase propeller 4 [structural motif]; Region:
Sialidase propeller 4"
/db_xref="CDD:271233"
misc_feature 7988..8149
/gene="HN"
/locus_tag="KM672_gp6"
/note="Sialidase propeller 5 [structural motif]; Region:
Sialidase propeller 5"
/db_xref="CDD:271233"
misc_feature 8168..8290
/gene="HN"
/locus_tag="KM672_gp6"
/note="Sialidase propeller 6 [structural motif]; Region:
Sialidase propeller 6"
/db_xref="CDD:271233"
gene 8430..15239
/gene="L"
/locus_tag="KM672_gp7"
/db_xref="GeneID:65102426"
CDS 8438..15205
/gene="L"
/locus_tag="KM672_gp7"
/codon_start=1
/product="polymerase"
/protein_id="YP_010087175.1"
/db_xref="GeneID:65102426"
/translation="MAGTREILLPEVHLNSPIVKHKLYYYILLGNLPNEIDLEDLGPL
HNQNWVQIANEESNLSQRLANVRNFLVTHIPGLRKGHWQESVSVILWPRVLPLIPEFK
IEDKLPLLKDWDKLVRESCAVINTGASQCIQDLSVGLTGRPNLFTRSRELSGDKRIID
LKSVVNAWHDSDWKRISDFWIMIKFQMRQLIVRQTENNDPELITFIENRNGILIITPE
LVVLFSTENCTLSYLTFEIVLMVSDMYEGRHNIISLCTVSTYLQPLRKRITYLLSLVD
NLAFQIGDSVYNVIALLESFVYAQLQMSDPIPELRGQFHAFVCSEIIDALRCTNHFTQ
DELRTITTNLISPFQGLTPDLTAELLCIMRLWGHPMLTASQAAGKVRESMCAGKVLDF
STIMKTLAFFHTILINGYRRKHHGVWPPLTLPDNASKGLTELMNDNTEISYEFTLKHW
KEISLIKFKKCFDADAGEELSIFMKDKAISAPKQDWMSVFRRSLIKQRHQQHQVPLPN
SFNRRLLLNFLEDDKFDPNVELEYVTSGEYLQDDTFCASYSLKEKEIKPDGRIFAKLT
KKMRSCQVIAESLLANHAGKLMKENGVVMNQLSLTKSLLTMSQIGIISEKARKSTKDN
INQPGFQSMKKNRNQLPKVTNLRDPGDDFELAASFLTTDLKKYCLQWRYQTIIPFAQS
LNRMYGFPHLFEWIHLRLMRSTLYVGDPFNPPTDTSQFDLDKVVNGDIFIVSPRGGIE
GLCQKAWTMISISVIILSATESGTRVMSMVQGDNQAIAVTTRVPRSLPTLEKKTIAFR
SCNLFFERLKQNNFGLGHHLKEQETIISSHFFVYSKRIFYQGRILTQALKNASKLCLT
ADVLGECTQSSCSNLATTVMRLTENGVEKDICFFLNIYMTIKQLAYDIIFPQVSVPGD
QITTEYINNPHLVSRLALLPSQLGGLNYLSCSRLFNRNIGDPVVSAVADLKRLIKAGC
MDYWVLYNLLGRKPGNGSWATLAADPYSINIEYQYPPTTSLKRHTQQALMELSTNPML
RGIFSDNAQAEENSLARFLLDREIVFPRVAHIIIEQTSVGRRKQIQGYLDSTRSIMRK
SLEIKPLSNRKLNEILDYNINYLAYNLALLKNAVEPPSYLKAMTIETCSIDIARSLRK
LSWAPLLGGRNLEGLETPDPIEITTGALIVGSSYCEQCAAGDTRFTWFFLPSGIEIGG
DPRDNPPIRVPYIGSRTDERRVASMAYVRGASSSLKAVLRLAGVYIWAFGDTLENWID
ALDLSHTRVNISLEQLQSLTPLPTSANLTHRLDDGTTTLKFTPASSYTFSSFTHISND
EQYLTINDKTADSNIIYQQLMITGLGVLETWNNPPISRTFEESTLHLHTGTSCCVRPV
DSCIISEALTVKPHITVPYSNKFVFDEDPLSEYETAKLESLSFQAQLGNIDAIDMMGK
LTLLAQFTARQIINSITGLDESVSLTNDAIVASDYVSNWISECMYTKLDELFMYCGWE
LLLELSYQMYYLRVTGWSNIVDYSYMVLRRIPGAALNNIASTLSHPKIFRRAINLDIV
APLNAPHFASLDYIKMSLDAIMWGCKRVINVLSNGGDLELVVTSEDSLILSDRAMNLI
ARKLTLLALVHHNGLELPKIKGFSPDEKCFTLTEFLQKVIHSGLSSIDNTSNFMYNVE
NPRLAAFASNNYYLTRKLLNSIRDTESGQLAITSYYESLEYVDSLRLIPHIPGSSCVE
DDSLCINDYIIWIIESNIDLEKYPIPRSPEDNSNFHNFKLNAPAHHTLRPLGLSSTAW
YKGISCCRYLERLKLPQGDHLYIAEGSGASMTIIEYLFPGRKVYYNSLFSSGDNPPQR
NYAPMPTQFIESVPYKLWQARTEQYPDIFEDFIPLWNGNAAMTDIGLTSCVEFIINKV
GPKTCSMVHVDLESSASLNQQCLSKPVINAIITATTVLCPRGILILKYSWLPFSRFST
LMTFLWCYFERITVLGSTYSDPANHEVYLICVIANNFAFQTVSQATGMALTLTEQGFT
LIAPEKINQYWDEHLKQERVVAEAIDKVVLGENALFNSGDNELILKCGGSPNARNLIE
IEPVSTFIELEQLVGTMLTTHFKEIIDITRSGTQDYESLLLTPYNLGLLGKISTIVRL
LTERILNHTIRNWLILPHSIRAMVKQDLEFGIFRITSILSADRFLKLSPNRKYLVSLL
TAGYIKRLIEGDCNIELTRPIQKQIWKAIGCVVYCQDPGEQYNPASFTDVNIHEDIER
GIDGEEL"
misc_feature 8468..11791
/gene="L"
/locus_tag="KM672_gp7"
/note="Mononegavirales RNA dependent RNA polymerase;
Region: Mononeg_RNA_pol; pfam00946"
/db_xref="CDD:395756"
misc_feature 12152..15112
/gene="L"
/locus_tag="KM672_gp7"
/note="mRNA capping enzyme, paramyxovirus family; Region:
paramyx_RNAcap; TIGR04198"
/db_xref="CDD:275046"
misc_feature 15240..15270
/note="trailer sequence"
ORIGIN
1 accaagggga aaatgaagtg gggtatgaaa tcatcgaaca ccccaaggat tacataggcc
61 cggaacacat ggccgtcgcg atcgacgccg acccagagta gttcgtaagt ggcctttact
121 ttacccctct atcactttaa ataatatcat tctcacaatg tcctccgtac tcaaagcata
181 tgagcggttt acgctcactc aagaactaca agatcaaagt gaagaaggaa ctattccccc
241 taccacacta aaacctgtca tcagagtatt cattctgact tcgaataatc ccgagctacg
301 ttcaaaactc ctgctatttt gcctacgcat tgtgctgagt aatggagctc gtgactccca
361 tcgatctgga gcattactca ctatgttttc attgccatca gcagctatgc ttaatcatgt
421 gaaattggct gaccaatcac ctgaggcaga catagagcgt gtggaaatag atggtttcga
481 agatgactca ttcagactca ttcctaatgc ccgctcaggc atgggaagag gggagataaa
541 tgcttacgct gcacttgcag aggatcttcc agatacgcta aatcatgcca ccccgtttgt
601 cgatccagat gttgagaaca caccatggga tgaaattgag acatttcttg atatgtgcta
661 cagtgtcctc atgcaggcat ggattgtcac ctgcaagtgc atgacagctc ctgaccaacc
721 tgtggcctcg attgagaaac gacttcaaaa gtatagacaa caaggaagaa ttaatccgag
781 atacctactt caacctgagg caagaagaat catccaggct ataatcagaa agggaatggt
841 tgtcagacat tttctaacat ttgagttaca gcttgcaagg gcgcagagcc tggtgtccaa
901 tcgttattat gccatggtgg gagacgtcgg gaagtatatt gagaattgtg gaatgggcgg
961 attcttcctt acactaaagt atgcacttgg aacaaggtgg ccaacactag ctcttgcagc
1021 tttttcagga gaattgacca aggttaagtc tctcatggca ctgtatcaga cattaggcga
1081 ccaggctcga taccttgcat tgctcgaatc cccgcaccta atggactttg ctgctgcaaa
1141 ttacccgctt ttgtatagtt atgcaatggg gattgggtat gttctggatg caaacatgag
1201 aaattatgca ttctcaagat cttacatgaa taaaacatat ttccagttgg gtatggaaac
1261 tgcaaggaaa caacaggggg ctgtggatat gcgtatggca gaggatcttg gtcttactca
1321 ggcagaaaga gcagagatgg ccaatactct cgcaaagttg actacagcta atcgtggtgc
1381 agatgcacgg ggtggagtga atccatttgc tgctgttgca ggacccagtc aacctcaagc
1441 atcagctgtc agtgatacat tcgaaagcta tatggcagca gaccgaatgc gtcagagata
1501 tgcagaagct ggagcctatg acaacgagat gcccccattg gaggaggaag aagaggatga
1561 caccatggga gtctctagga ctggaccaac cctggaacag gttgccattg atatccagaa
1621 caatccgccc agtggaccga ccaacaatga tgatctgaac gctgctctcg gtgatcttga
1681 catctaaaga atgtacaact gttccttcag attcacaaac atccccaatc taatataaca
1741 gtcaaattgc acaatagcat tgtttatcta attcataatc tttaagaaaa aaataggccc
1801 ggacgggtta gcaagacagc aattaaatca gtgcaaaccc cagggctacc aaattatgga
1861 tcctaccgat ctaagctttt cctctgagga gatcaataaa ctcatagaga ctggactaaa
1921 cactgtagag tattttactt ctcaacaagt taccggccaa tcatctcttg ggaagaattc
1981 aattcctccc ggggtcaccg gaatactaac caatgcagca gaagcgaaga tacaggaatc
2041 cattgctccc attaaacccg gcactggggg cggagcgaga ccaaagaagg ctcgtcctaa
2101 aattgccata gtgccagctg atgacaagac cgtcccagga aaacccatac ccaaccctct
2161 acttggcttg gattctactc ctagcactca atcagtcctc gatctaagtg gtaagactca
2221 tccaacaggg tcatacaaag gagtaacatt ggctaaattt ggaaaagaaa atctaatgac
2281 cagatttatg gaggaaccca gagagagtcc cattgctact agttccccag ttgattttaa
2341 gaggggcagg ggtaccggtg gttaccatag aagggaatac tctatcggat gggtgggaga
2401 tgaagtcaaa gtcacagagt ggtgcaatcc atcctgctcc ccaatcacag cagcagcaag
2461 gagatttgaa tgcacttgta accagtgtcc agtcacttgc tctgaatgtg agcgagatac
2521 ttaatacagt gagagggatg gatacccggc tcaaccaaat tgagtctaaa gtggatagga
2581 ttatgtcatc acaatctctt cttcagacca tgaagaatga cattatagga ctcaaggctg
2641 gcatggcaac actagaagga atgatcacta ctgttaagat aatggaccct ggtgtcccca
2701 gtaatgtgac tgtagaggat gtgcgtaaga cactaagcaa tcatgcagtg gttgtgccgg
2761 agtcattcaa tgatagcttc cttacccaat ctgaggatgt gctatcattg gatgatctag
2821 ctcgtcccac tgcaaccaat gtcaaaaaga tagtcagaaa agttccgcct ccgaaagatc
2881 taacagggtt gaaaattact cttgagcaat tggcaaagga ctgcatcagc aaaccaaagg
2941 caagggaaga attcctgatc aaaatcaatc aagcgacaag tgaaagtcag ctgcatgatc
3001 tgaagaagtc aatcattcgc agcgcaattt aaacttctca cctgacaaat caagactcga
3061 acaaactaga ggattcagca gcttagattt agaaaaaaca taaatcacta gatagcaccc
3121 acagccctga taggcccgaa cactacatca actctccact aaccatgccg tccataagca
3181 ttccagctga tcccacaaat cctcggcaat caatcaaggc attccccatc atcataaaca
3241 gcaataatgg agagagggga cgtctggtca aacaactccg cacaacatat ctcaatgacc
3301 tggatgctca tgagccgcta gtgacatttg taaacacata tggattcatt tatgaacaag
3361 atcgagggaa tactatcatc ggggaagacc agttgggaaa aaggagagaa gctgtcacgg
3421 cggccatggt cacatttgga tgtggtccta accttccctc acttggcaat atcttgaatc
3481 aacttaatga tttccaagtg gttgtcagaa agacttctag caaagcagag gaaatagtat
3541 ttgaggttat caaacatccc cgtatcttca ggaatcatac actaattcag aagggggttg
3601 tatgtgtatc agctgaaaag tttgtaaaat cacctggtaa aattcagtct ggtatggatt
3661 atgttttcat cccaaccttc ctatctgtaa cttattgccc tgcagcaatc aagtttcagg
3721 ttccgggtcc catgcttaaa atgagatcca gatacaccca aagccttcaa ttggagctca
3781 tgattcgaat tttatgcaag ccagactctc cactaatgaa ggtccacaca cctgacaagg
3841 acggacgcgg ttgcttggtg tcggtctggc tgcatgtctg caacatattc aaatcgggca
3901 atagaaacgg gagtgaatgg caggagtatt ggatgaggaa gtgtgccaac atgcaacttg
3961 aggtgtctat cgcagatatg tggggtccga ctataatcat tcatgcacga ggccacatcc
4021 ccaaaagtgc aagattgttc tttgggaaag gaggatggag ttgtcatcca ttgcatgagg
4081 tggtacccag tgtgacaaag accctttggt cagttgggtg tgagatcact aaagccaagg
4141 ctatcatcca agagagtagt atctcactct tggtagagac tacagacatc atcagtccga
4201 aagtcaaggt atcgagcaaa cataagagat tcgggaaatc aaattggggt ctattcaaga
4261 aaacaaaatc acttccaaat ctcacggagt tggaatgaag tacattctac tacatacttg
4321 gagagttgca cttaagcctg ctcatcaatt gttattgatt acactacaga taatatgtat
4381 aatcatatct cttgctgcta ccactacatc tacttacact actaataagc aacctgatca
4441 tcactacatt agattgtcaa gacaattaac atgttagttg gttgattaat agacaaattc
4501 aaagaaaaag ctaataagaa atgatattat aagcacgaat caaatagtcc ttcttaacta
4561 tggcgtctct attggttggt ctgatgctgt tggggctgac tacaggctct accgggctag
4621 acccgactgc actaatgcag atcggggtta ttcctaccaa tgtacgacag ctaatgtatt
4681 acacagaagc atcatcatcc tatattgtag tcaagttgat gcccactatc gattctccaa
4741 ttagtgggtg caatatcagc tctattgcaa gttacaactc tactataacc aaactcctac
4801 aacccattgg agagaacttg gagaccatta aaagtcaatt agtccctact cgacgacgcc
4861 gcaggtttgc aggtgttgtg atagggttag ctgcattggg agtggcaacc gcggcacaag
4921 taactgcagc catagctctg gtaaaggcaa atgaaaacac agcggctatt ttaaacctta
4981 aagcttcaat ccagaaaacc aatgctgcag tggcagatgt cgtccaagcg acacagtcgc
5041 tcggcactgc agtccaagct gtccaagacc atctcaacaa tgtggtgagt cctgctatta
5101 cagcagctaa ctgtaaagcc caagatgcta tcattggatc catcctaaac atgtatttga
5161 ctgagttaac caccatcttt cacaatcaaa tcactaatcc agcactgact cccattacaa
5221 tccaagcatt aagaatcctc ctgggtagta ctcttccaac tgttgtagag aaagcattca
5281 atactcagat cagtgctgct gaattactat cttcaggatt gttaacaggt caaattgtcg
5341 gacttgacct aagctatatg cagatggtca ttaaagttga cttaccgaca ctcactaccc
5401 agcctgctac tcaaattgtt gacctagcaa ctatatcagc cttcattaac aatcaagagg
5461 tgatggcaca attgccaaca cgaattatgg tcactggagg tctgatccag tcttatcctg
5521 catctcagtg tactatcact ccatctactg tctattgtcg atataatgat gcacaagtgc
5581 tttccgagga cacagttgct tgtctacaag gcaatctcac aagatgtaca ttctctccgg
5641 ttgtaggtag ttttatgaca agatttgtac tgtttgacgg cattgtgtat gctaactgcc
5701 ggtctatgct ctgtacatgt atgcaaccag cttcagtaat actgcaacca aattcgtctc
5761 caataactgt aatagatatg tataaatgtg tcagccttca gctagataac ctacgcttca
5821 caatcacaca aattgcaaat gctacttaca atagtacgat taaattgagt gcatctcaaa
5881 tcctcccaat cgaccctcta gatatatccc aaaatctagc agcagtaaac aagagtctaa
5941 atagtgcact ccaaaacttg gctcaaagcg agaattacct atcagctatt acctctgcaa
6001 acactactag catactggcc attattgccc tctgcattgg cattctgggg ctcctattag
6061 tcatcctgat agccgtagtg atatggaaat tagtgtcaat agtatccact gagcgaacta
6121 gaatggataa ctatgtatac cacaatcagg cattccaaca atctcagtta gatctcaata
6181 aaccataaat aagtctttca cctatcaggg cataaattga ctatcccttt actgaattta
6241 agaaaaaatt actaggaccg aacctagtat tgaaagaacc gtctcggtca atctaggcaa
6301 ctgggcaaga accgtctcgg aacgcccaaa acatgctgtc tgacccagaa tcccaaaact
6361 ccaatgaaaa cacaaaaaaa gctggcaatt tcattatttg tttgttattt atcttcttct
6421 tgcttatcac gttcatagta cctgcaatcc gctattttct gtcatagggg ttatcccacc
6481 ttgtagttac tgtaggctta atcccttggc caatccgcac tgagctttta agaaaaaaat
6541 aggcccgaac atatagacca ccactctcga cactttccac ttaacactaa tactatctaa
6601 cactacaatg ggggcagatg aagctccttt gaaaggcact tgtcgtgtct tattcagaac
6661 cactaccctg ctattacttt gcactttact ggccttaagt gttgcagtac tttatgaagt
6721 attgattact caacaatact taaagaacaa ctctgggtca tccaacctac cttctggatt
6781 gtctggaata actgattctc tggccaacat attgtctgta gttaaccaga ttgtttataa
6841 ttcagctgtg gcaatcccac tccagctcga cacgatggag tcaacactac tcactgctat
6901 caagtcactc caaaccagtg acaaattgga gcctaattgt tcttggggcg cagctttgat
6961 caatgataac aggtacattc acggaatcaa tcagttttat ttctcaccaa cggaaggcca
7021 aaacctgacc ctgggccccc tattaaacat tccaagtttt atacctactg ctacaacccc
7081 agaaggttgc actaggatac cctctttttc actaacaaag acacattggt gttacactca
7141 caatgtaata ctaaatggct gtcttgatca tgtttcctct aatcaatttg tatcaatggg
7201 aataatcgaa cctacttcag caggatttcc atccttccgt acgctcaaga cattgtactt
7261 gagtgacgga gtaaacagaa agagttgctc tatcagtacc atcccaggcg gttgtatgat
7321 gtactgtttt gtgtcgacac agtcagagaa agatgattat ctttctgctg ccccacctga
7381 acagagaatc atcatgatgc attacaatga ctccattgtt gagaggataa tcaatccacc
7441 aggtgtgctt gatgtctggg caacattgac tcctggaact ggaagtggag tttattacct
7501 tggctgggtt ttattcccgg tatacggtgg ggtaattagg gggactaatc tctggaacag
7561 ccagaataac aagtacttta tttccaagat ggcatcgact ctttgcaagc aagaccaggc
7621 aactcaaatt gctaatgcta agaactcata ttatagtagt tggttcgggg atcgcttaat
7681 tcaagctggt attcttgctt gcccactaca aagagacttg actaatgaat gtttagttct
7741 tccgtactct aatgaccaag tcatgatggg tgcggaagga agattatata tttatggtga
7801 ctcattatat tattaccaac gtggaactag ttggtggccg atgacaatgc tttataaatt
7861 gacattgaca ttttccaaca accagccatc tgccattaca gcacaaaata tacccactca
7921 gcaagtccct agacctggga ctggaggttg ttcagcagat aacagatgtc cgggtgtttg
7981 tctgactgga gtttatgcgg acgcctggct acttactgat cccgcagcag ttaactcatt
8041 tggctcccag ataattttca ctggatcata tctcaattca gcaacccaac ggattaatcc
8101 gaccatgtat attgcaaata ataccaaaat actaaactct caacaattcg gtgccaatgg
8161 tcaaggtgct tcttatggtc acactacatg cttccgggat actggatctg taaatgtgta
8221 ctgtatttac atcatagagg tctcatcttc cctccttggg caattccaga ttgttccatt
8281 tgttcggcag atcaatctta attaaaaaac ctttcccata cagactcaag attctaatct
8341 attcaaacac aagaagatag agaacaacct ctaccgcatt taccacatca gagctccgtg
8401 attatttaag aaaaaacata gcattaatta ggccagaatg gctgggactc gggagatctt
8461 actccctgaa gttcatttga attctccaat tgttaaacat aagctttatt attatatact
8521 tcttggaaac ctaccaaatg agatagatct agaagactta ggccctctgc ataatcaaaa
8581 ttgggttcag attgcgaatg aagagtccaa cttatcacaa cgattagcca atgtcaggaa
8641 ctttctggtc acccatatcc ctggtctaag aaaaggtcac tggcaagaat ctgtaagtgt
8701 catcctctgg cctagggttt tgcctttgat ccctgagttc aagattgagg ataaattacc
8761 cctacttaaa gattgggata agcttgttag agaatcatgt gctgttatta atacaggtgc
8821 atcacaatgt attcaagatt taagtgtagg cctaactgga cgaccaaatc tatttaccag
8881 atcccgagag ttgtcaggag ataaacgaat catagatttg aaatctgttg ttaatgcatg
8941 gcatgattca gattggaaga gaatcagtga tttttggatt atgatcaaat tccaaatgag
9001 acaacttata gtccgccaaa ctgaaaataa tgatcctgaa ctaattacat tcatagagaa
9061 taggaatggg atcctaatta ttaccccaga attagttgtc ctattcagca cagagaattg
9121 cactttatca tatctaacat ttgaaattgt gttaatggtg tctgatatgt atgagggacg
9181 acacaatatt atttcactat gcactgtcag tacctatctt caaccccttc ggaaacgcat
9241 tacatattta ttgagtcttg ttgacaatct tgctttccaa atcggagatt cagtgtataa
9301 tgtcattgcc ttgctagaat catttgtata tgcccaactg caaatgtctg accccattcc
9361 ggaactacgc ggacaattcc atgcttttgt ttgttcagaa attattgatg cactaagatg
9421 cactaaccac tttactcaag atgaattgag gaccatcact acaaatctaa tatcaccttt
9481 ccaaggtcta acaccggatc taacagcgga gctattatgt attatgagat tatggggtca
9541 tccaatgctc actgcaagcc aagcggccgg aaaggtacga gaatcaatgt gtgcagggaa
9601 ggtattagat ttttctacaa taatgaagac tctagcattc ttccacacaa ttctcattaa
9661 tggttataga agaaagcatc atggtgtttg gccacctctt accctacctg acaatgcttc
9721 aaagggactc acagagctaa tgaatgacaa cacagaaata agttatgaat tcactctaaa
9781 acattggaaa gaaatctctt taattaaatt caaaaaatgc tttgacgcag atgcaggaga
9841 agaactcagc atctttatga aagataaagc tatcagtgct ccgaaacaag attggatgag
9901 tgtttttagg aggagcttga tcaaacaaag acaccaacag caccaagtac ccctacctaa
9961 ttcatttaat agaagattat tattgaattt tcttgaagat gataaatttg accccaatgt
10021 ggaattggaa tatgtgactt caggcgaata tctccaggat gatacattct gtgcctccta
10081 ctcacttaaa gagaaggaaa tcaagcccga tggccggatt tttgcaaaat taacaaagaa
10141 gatgcgatcc tgccaagtaa tagcagaatc cctattagct aatcatgctg ggaagctgat
10201 gaaagagaat ggtgtggtaa tgaatcaatt gtccctaaca aaatcactgc ttactatgag
10261 tcagattggc attatatccg aaaaggcaag aaaatcgact aaggataaca ttaaccaacc
10321 aggatttcaa agtatgaaga agaataggaa ccagttgccg aaagttacaa atctgagaga
10381 tcctggggat gactttgaac tcgcagcttc ctttctaact actgacctca agaagtactg
10441 cttacaatgg aggtatcaaa caattatccc atttgcacaa tctctcaatc gaatgtatgg
10501 atttccccat ctatttgaat ggatccacct ccgcttaatg agaagcactc tatatgtagg
10561 agatccattc aacccaccaa ccgacacaag tcaatttgat ttagacaagg tggttaatgg
10621 tgacatcttt attgtgtcgc caaggggcgg tattgaaggc ctgtgtcaaa aggcttggac
10681 tatgatctca atttctgtta taatcttgtc tgcaacggag tctggtactc gagtgatgag
10741 tatggtacaa ggagacaatc aagctatagc cgtgactaca agagttcctc gcagccttcc
10801 aacacttgag aagaagacaa ttgccttcag atcctgcaac cttttctttg agcggctgaa
10861 acagaacaat tttggcttag gacaccactt aaaggagcaa gagaccatca taagttccca
10921 cttctttgta tatagcaaaa gaattttcta tcaagggaga atactcacac aggcactcaa
10981 aaatgcaagc aaactatgtc ttacagcaga tgtgttagga gagtgcactc aatcgtcctg
11041 ttcaaatctg gcaaccactg ttatgcgcct tactgagaat ggagttgaaa aagacatctg
11101 tttcttcctc aacatctata tgactatcaa acagctagca tatgatataa tatttccaca
11161 ggtgtcagtt ccaggggacc agataaccac ggaatacatc aataatcctc atttagtatc
11221 tagattggca ttacttccat ctcagttagg aggtcttaat tacctttcat gcagcagatt
11281 atttaaccgg aatatcggag accctgtagt ctctgctgtt gcagatttaa agcgcctaat
11341 taaagctggt tgtatggatt attgggtact ttataaccta ttgggtagaa aacctgggaa
11401 tggctcatgg gcaaccttag ctgctgatcc ttactcaatc aatatagaat atcaatatcc
11461 acctactaca tcactcaagc gtcatactca acaagcgctg atggaattga gcactaatcc
11521 catgttacgt ggcatcttct cagataatgc ccaagcagaa gaaaatagcc tggctagatt
11581 cctactagat agagaaatag tgtttccccg agtcgcccac atcatcatcg agcagacaag
11641 tgtaggcaga agaaaacaga tccaaggcta tttggactca acccgatcga tcatgagaaa
11701 atctcttgag atcaagcctt tgtccaacag aaaattgaac gagatcctcg attataacat
11761 caattatctt gcttacaatc ttgctcttct caaaaatgca gtagaaccgc catcatacct
11821 taaagcaatg actattgaaa catgtagtat tgacattgca aggagtttaa gaaagctgtc
11881 atgggctccc ttacttggag gcaggaatct tgaaggacta gagactccag atcctattga
11941 gatcaccact ggtgcactca ttgttggatc cagttattgt gaacaatgtg cagctggaga
12001 cacgaggttc acatggttct ttctcccatc aggaattgaa attgggggtg atccccgtga
12061 taacccaccc atccgtgtcc catacatagg atcaaggacg gatgaacgcc gtgttgcatc
12121 tatggcatat gtgcggggtg cttcaagtag tcttaaagca gtattgaggc ttgctggtgt
12181 ctatatttgg gcatttggtg atacactgga gaattggatt gatgcacttg atctatctca
12241 tactagagtt aatatttcgt tagaacaatt gcaatctctc acaccactcc caacatctgc
12301 aaatctaaca catcgtctag atgacggaac aactacctta aaatttacac cagctagctc
12361 ttataccttt tcaagcttta cacatatttc taatgatgag caatacctaa ctattaatga
12421 taagactgct gactcaaaca tcatctatca acaacttatg atcacaggtc taggagtgtt
12481 agaaacatgg aataatcccc ccattagtag gacctttgag gaatctacac tccacttgca
12541 caccgggacc tcttgttgtg tgcgccctgt agattcatgt attatatcag aggctctcac
12601 agtcaaaccg catattacag taccctacag caacaaattt gtcttcgatg aggatcccct
12661 ctcagaatat gagacagcaa aacttgagtc tctgtctttc caagcacaat tgggtaacat
12721 tgatgccatt gatatgatgg ggaagttaac attattagca caatttacag caaggcaaat
12781 tattaactca atcactggac tggatgaatc agtttcactt acaaacgatg ccatagtggc
12841 atctgactat gtatcaaatt ggataagtga gtgcatgtat actaagctag atgaattatt
12901 tatgtattgt ggatgggaat tgttgttaga gttatcttat caaatgtatt acttgagggt
12961 gactgggtgg agcaatattg tagattactc atatatggta ctccgtcgca tacctggtgc
13021 tgcattaaat aacattgcat cgactttgag ccatcccaag attttccgga gagccatcaa
13081 tcttgatatt gttgcaccac ttaatgcgcc tcattttgca tctcttgact atatcaagat
13141 gagcctagat gccatcatgt ggggatgtaa gcgagtaata aatgttttat ctaatggggg
13201 tgacttagaa ttagtagtaa catcagaaga tagtctgata ctgagtgata gagctatgaa
13261 cttgatagca cggaaattga cactgctagc tctggttcat cacaatggat tagaattacc
13321 caaaatcaaa ggattttccc ctgatgaaaa atgtttcaca ttaacagaat tcttacagaa
13381 agtaatacac tcaggtctga gttccattga taatacatcc aatttcatgt acaacgtgga
13441 gaatcctcgg ctcgctgcat ttgccagtaa taattattac ttaacccgaa aactgcttaa
13501 ttctataaga gatactgaat caggacaact tgcaatcaca tcttattatg aatcacttga
13561 atatgtagat agtctgagac tcattccaca tatccctggc tcatcctgtg ttgaagatga
13621 cagtttatgc attaatgatt atatcatctg gattattgaa tccaatatcg acttagaaaa
13681 gtacccaata cctcgcagcc ctgaagacaa ctctaacttc cataatttca aattgaacgc
13741 ccctgcacac catacattac gacccctcgg attatcctca actgcatggt ataaagggat
13801 tagctgttgc agatatcttg aaagattaaa actaccacaa ggggatcatt tgtatattgc
13861 tgaggggagt ggtgctagta tgacaattat agagtatctg tttccaggaa gaaaggtgta
13921 ttacaactct ctatttagta gtggtgataa cccaccacaa cggaattatg caccaatgcc
13981 gacccaattc attgaaagtg tcccgtacaa attatggcag gctcggacag agcaatatcc
14041 tgacatattt gaagacttca taccactttg gaatggtaat gcagcaatga cagacatcgg
14101 acttacatct tgtgtagaat ttatcattaa taaggttgga ccaaaaactt gcagtatggt
14161 tcatgtagat ctagagtcaa gtgcaagctt aaatcaacaa tgtctatcaa aaccagtgat
14221 taatgccatt atcacagcta ccacagtact gtgtccgagg gggatattga tcttgaagta
14281 tagttggcta cctttttcac ggttcagtac actaatgaca ttcctgtggt gctattttga
14341 aaggattact gtcctgggaa gtacttactc tgatcctgct aaccatgagg tgtatctcat
14401 ttgtgtaata gccaacaatt ttgcattcca gactgtctct caagctactg gtatggcact
14461 gactctgact gagcaaggtt tcaccctaat tgctcccgag aagattaatc aatattggga
14521 cgaacaccta aaacaagaaa gagtagtagc agaagccatt gacaaggttg tcttaggaga
14581 aaatgcatta tttaactcgg gtgataatga attgatcctt aaatgtggcg gctccccaaa
14641 tgccagaaat ctgatagaga ttgaaccggt ctctaccttc atagaattag aacagcttgt
14701 gggaactatg ttgaccactc atttcaaaga aattattgac atcactcgat ctggaacaca
14761 agattatgaa agtctcctcc tcacacccta caatctgggt ctcttaggta aaattagtac
14821 tattgtaaga ttgctgactg agcgaatcct taatcatact atccggaatt ggttaatttt
14881 gcctcattcc attagagcca tggtcaaaca ggatctggaa tttggaatct tccgaataac
14941 ctcaatccta agtgctgatc gattcctcaa attgtctcca aacaggaaat atctagtctc
15001 cttactaaca gctgggtaca tcaagagatt aattgagggc gactgcaaca ttgaactaac
15061 tagaccaata cagaaacaga tctggaaggc aattgggtgt gtggtttact gtcaggatcc
15121 tggggaacaa tataacccgg cttccttcac tgatgttaac atacatgaag acatcgagcg
15181 cgggatcgat ggcgaggagt tgtaatcaga tttaaattag gatattattt aagaaaaaat
15241 aagaagacct atcttgattt tccccttggt
//
