LOCUS NC_075011 11604 bp RNA linear VRL 05-MAY-2023
DEFINITION Madariaga virus strain PE-3.0815, complete genome.
ACCESSION NC_075011
VERSION NC_075011.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Madariaga virus
ORGANISM Madariaga virus
Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
Martellivirales; Togaviridae; Alphavirus.
REFERENCE 1 (bases 1 to 11604)
AUTHORS Kondig,J.P., Turell,M.J., Lee,J.S., O'Guinn,M.L. and
Wasieloski,L.P. Jr.
TITLE Genetic analysis of South American eastern equine encephalomyelitis
viruses isolated from mosquitoes collected in the Amazon Basin
region of Peru
JOURNAL Am. J. Trop. Med. Hyg. 76 (3), 408-416 (2007)
PUBMED 17360860
REFERENCE 2 (bases 1 to 11604)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (03-MAY-2023) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 11604)
AUTHORS Kondig,J.P., Turell,M.J., Lee,J.S., O'Guinn,M.L. and
Wasieloski,L.P. Jr.
TITLE Direct Submission
JOURNAL Submitted (13-OCT-2005) Diagnostic Systems Division, USAMRIID, 1425
Porter St., Fort Detrick, MD 21702, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to DQ241303.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..11604
/organism="Madariaga virus"
/mol_type="genomic RNA"
/strain="PE-3.0815"
/host="mosquito"
/db_xref="taxon:1440170"
/country="Peru"
/collection_date="1996"
5'UTR 1..45
gene 46..7470
/locus_tag="QJ817_gp1"
/db_xref="GeneID:80512381"
CDS 46..7470
/locus_tag="QJ817_gp1"
/codon_start=1
/transl_except=(pos:5623..5625,aa:OTHER)
/product="nonstructural protein"
/protein_id="YP_010775794.1"
/db_xref="GeneID:80512381"
/translation="MEKVHVDLDADSPYVKLLQKCFPHFEIEATQVTDNDHANARAFS
HLATKLIESEVDPDQVILDIGSAPVRHTHSKHKYHCICPMISAEDPDRLQRYADKLRK
SDVTDRFIASKAADLLTVMSTPDVETPSLCMHTDSTCRYHGTVAVYQDVYAVHAPTSI
YHQALKGVRTIYWIGFDTTPFMYKNMAGAYPTYNTNWADESVLEARNIGLCSSDLHEK
RLGKISIMRKKKLQPTNKVVFSVGSTIYTEERILLRSWHLPNVFHLKGKTSFTGRCNT
IVSCDGYVVKKITISPGIYGKVDNLASTLHREGFLSCKVTDTLRGERVSFPVCTYVPA
TLCDQMTGILATDVSVDDAQKLLVGLNQRIVVNGRTQRNTNTMQNYLLPVVAQAFSRW
AREYRADLEDEKDLGVRERSLVMGCCWAFKTHKITSIYKKPGTQTIKKVPAVFNSFVI
PQFNSYGLNIGLRRRIKMLLEEKRKPAPIITEADVAHLKGMQEEAEAVAEAEAVRAAL
PPLLPEVERETIEADIDLIMQEAGAGSVETPRRHIKVTTYPGEETIGSYAVLSPQAVL
NSEKLACIHPLAEQVLVMTHKGRAGRYKVEPYHGRVVVPSGTAIPIPDFQALSESATI
VYNEREFVNRYLHHIAINGGAINTDEEYYKVLRSSEADSEYVFDIDARKCVKKADAGP
MCLVGELVDPPFHEFAYESLKTRPAAPHKVPTIGVYGVPGSGKSGIIKSAVTKRDLVV
SAKKENCTEIIKDVKRMRGMDIAARTVDSVLLNGVKHPVDTLYIDEAFACHAGTLLAL
IAIVKPKKVVLCGDPKQCGFFNMMCLKVHFNHEICTEVYHKSISRRCTKTVTAIVSTL
FYDKRMRTVNPCSDKIIIDTTSTTKPQRDDIILTCFRGWVKQLQIDYKNHEIMTAAAS
QGLTRKGVYAVRYKVNENPLYAQTSEHVNVLLTRTEKRIVWKTLAGDPWIKTLTAHYP
GEFSATLEEWQAEHDAIMERILETPASSDVYQNKVHVCWAKALEPVLATANITLTRSQ
WETIPAFKDDKAFSPEMALNFLCTRFFGVDIDSGLFSAPTVPLTYTNEHWDNSPGPNR
YGLCMRTAKELARRYPCILKAVDTGRLADVRTNTIKDYSPLINVVPLNRRLPHSLVVS
HRYTGDGNYSQLLSKLIGKTVLVIGTPISVPGKRVETLGPGPQCTYKADLDLGIPSTI
GKYDIIFVNVRTPYKHHHYQQCEDHAIHHSMLTRKAVDHLNKGGTCVALGYGTADRAT
ENIISAVARSFRFSRVCQPKCAWENTEVAFVFFGKDNGNHLRDQDQLSIVLNNIYQGS
TQYEAGRAPAYRVIRGDISKSTDEAIVNAANNKGQPGAGVCGALYKKWPGAFDKVPIA
TGTAHLVKHTPNIIHAVGPNFSRVSEVEGNQKLSEVYMDIAKIINRERYNKVSIPLLS
TGIYAGGKDRVMQSLNHLFTAMDTTDADVTIYCLDKQWEARIKDAIARKESVEELVED
DKPVDIELVRVHPLSSLVGRPGYSTDEGKVHSYLEGTRFHQTAKDIAEIYAMWPNKQE
ANEQICLYVLGESMTSIRSKCPVEDSEASSPPHTIPCLCNYAMTAERVYRLRMAKNEQ
FAVCSSFQLPKYRITGVQKIQCNKPVIFSGVVPPAIHPRKFSAIEETVPVTIERLVPR
RPAPPVPVPARIPSPRCSPAVSMQSLGGSSTSDVVISEAEVHDSDSDCSIPPMPFVVE
AEVHASQGSHWSIPSASGFEIRELPEDRSISGSPTRTSVISDHSVNLITFDSVTDIFE
NFKQAPFQFLSEIRPIPAPRRRVGGFETDTKRYDKTEEKPIPKPRTRTTKYKQPPGVA
RSISEAELDEFIRRHSNXRYEAGAYIFSSETGQGHLQQKSTRQCKLQNPILERSVHEK
FYAPRLDLEREKLLQKKLQLCASEGNRSRYQSRKVENMKAITAERLLQGIGAYLSAES
QPVECYKVNYPVPIYSTTRSNRFSSADVAVRVCNLVLQENFPTVASYTITDEYDAYLD
MVDGASCCLDTATFCPAKLRSFPKKHSYLRPEIRSAVPSPIQNTLQNVLAAATKRNCN
VTQMRELPVLDSAAFNVECFKKYACNDEYWDTFKNNPIRLTTENVTQYVTKLKGPKAA
ALFAKTHNLQPLHEIPMDRFVMDLKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLC
GIHRELVRRLNAVLLPNIHTLFDMSAEDFDAIIAEHFQFGDAVLETDIASFDKSEDDA
IAMSALMILEDLGVDQALLDLIEAAFGNITSVHLPTGTRFKFGAMMKSGMFLTLFINT
VVNIMIASRVLRERLTNSPCAAFIGDDNIVKGVKSDALMAERCATWLNMEVKIIDATV
GVKAPYFCGGFIVVDQVTGTACRVADPLKRLFKLGKPLPLDDDQDGDRRRALYDEALR
WNRIGITDELIKAVESRYEVFYISLVITALTTLAATVSNFKYIRGNPITLYG"
mat_peptide 46..1644
/locus_tag="QJ817_gp1"
/product="nsP1"
/protein_id="YP_010806420.1"
misc_feature 97..1161
/locus_tag="QJ817_gp1"
/note="Viral methyltransferase; Region: Vmethyltransf;
pfam01660"
/db_xref="CDD:396298"
mat_peptide 1645..4026
/locus_tag="QJ817_gp1"
/product="nsP2"
/protein_id="YP_010806421.1"
misc_feature 2194..2907
/locus_tag="QJ817_gp1"
/note="Viral (Superfamily 1) RNA helicase; Region:
Viral_helicase1; pfam01443"
/db_xref="CDD:366646"
mat_peptide 4027..5646
/locus_tag="QJ817_gp1"
/product="nsP3"
/protein_id="YP_010806422.1"
misc_feature 4072..4452
/locus_tag="QJ817_gp1"
/note="X-domain (or Mac1 domain) of viral non-structural
protein 3 and related macrodomains; Region:
Macro_X_Nsp3-like; cd21557"
/db_xref="CDD:438957"
misc_feature order(4090..4098,4108..4128,4342..4347,4351..4365,
4447..4449)
/locus_tag="QJ817_gp1"
/note="ADP-ribose binding site [chemical binding]; other
site"
/db_xref="CDD:438957"
mat_peptide 5647..7467
/locus_tag="QJ817_gp1"
/product="nsP4"
/protein_id="YP_010806423.1"
misc_feature 6094..7467
/locus_tag="QJ817_gp1"
/note="RNA-dependent RNA polymerase (RdRp) in the family
Togaviridae of positive-sense single-stranded RNA
[(+)ssRNA] viruses; Region: Togaviridae_RdRp; cd23250"
/db_xref="CDD:438100"
misc_feature 6739..6783
/locus_tag="QJ817_gp1"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438100"
misc_feature 6925..6996
/locus_tag="QJ817_gp1"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438100"
misc_feature 7018..7062
/locus_tag="QJ817_gp1"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438100"
gene 7544..11272
/locus_tag="QJ817_gp2"
/db_xref="GeneID:80512380"
CDS 7544..11272
/locus_tag="QJ817_gp2"
/codon_start=1
/product="structural polyprotein"
/protein_id="YP_010775795.1"
/db_xref="GeneID:80512380"
/translation="MFPYPTLNYPPMAPVNPMAYRDPNPPRRRWRPFRPPLAAQIEDL
RRSIANLTFKQRAPNPPAGPPAKRKKPAPKPKPAAPKKKRQPPPAKKQKRKQKPGKRQ
RMCMKLESDKTFPIMLNGQVNGYACVVGGRVFKPLHVEGKIDNEQLAAIKLKKASIYD
LEYGDVPQCMKSDTLQYTSEKPPGFYNWHHGAVQYENNRFTVPRGVGGKGDSGRPILD
NRGRVVAIVLGGANEGSRTALSVVTWNQKGVTVKDTPEGSEPWSLTTVMCVLANITFP
CDQPPCMPCCYEKNPHETLSMLEQNYDSQAYDLLLDAAVKCNGRRTRRDLETHFTQYK
LARPYIADCSNCGHGRCDSPIAIEDIRGDAHAGYIRIQTSAMFGLKSDGVDLAYMSFM
NGKTLKAIKIEHLYARTSAPCSLVSYHGYYILAQCPPGDTVTVGFQDGAIKHMCTIAH
KVEFKPVGREKYRHPPEHGVELPCTKYTHKRADQGHYVEMHQPGLVADHSLLSMSSTK
VKITVPSGSQVKYYCKCPDVKEGTTGSDYTTACTDLKQCRAYLIDNKKWVYNSGKLPR
GEGETFKGKLHVPFVPVTSKCTATLAPEPLVEHKHRSLVLHLHPEHPTLLTTRALGSN
ARPTRQWIEQPTTVNFTVTGEGFEYTWGNHPPKRVWAQESGEGNPHGWPHEIVIYYYN
RYPMTTVIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYRLAPNAQVPILLAVLCCVK
PTRADDTLQVLNYLWNNNQNFFWMQTLIPLAALIVCMRMLRCLLCCGPAFLLVCGALG
AAAYEHAAVMPNKVGIPYKALVERPGYAPVHLQIQLVTTKIIPSANLEYITCKYKTKV
PSPVVKCCGSTQCSAKSLPDYQCQVFTGVYPFMWGGAYCFCDTENTQMSEVYIERAEE
CSVDQAKAYKVHTGTVQAVVNITYGSVSWRSADVYVNGETPAKIGDAKLTIGPLSSAW
SPFDSKVVVYGHEVYNYDFPEYGTGKAGSFGDLQSRTLTSNDLYANTNLKLQRPQPGV
VHTPYTQAPSGFERWKKDRGAPLNDIAPFGCTIALDPLRAENCAVGNIPLSIDIPDAA
FTRIAETPTVSDLECKVTECTYASDFGGIATISYKASKAGNCPIHSPSGIAVIKENDV
TLADSGAFTFHFSTASIHPAFKMQVCTSVVTCKGDCKPPKDHIVDYPAQHTETYTSAV
SATAWSWLKVLVGSTSAFIVLGLIATAVVALVLFTHRH"
mat_peptide 7544..8326
/locus_tag="QJ817_gp2"
/product="capsid"
/protein_id="YP_010806424.1"
misc_feature 7856..8326
/locus_tag="QJ817_gp2"
/note="Alphavirus core protein; Region: Peptidase_S3;
pfam00944"
/db_xref="CDD:366379"
mat_peptide 8327..8515
/locus_tag="QJ817_gp2"
/product="E3"
/protein_id="YP_010806425.1"
misc_feature 8342..8515
/locus_tag="QJ817_gp2"
/note="Alphavirus E3 glycoprotein; Region:
Alpha_E3_glycop; pfam01563"
/db_xref="CDD:396236"
mat_peptide 8516..9775
/locus_tag="QJ817_gp2"
/product="E2"
/protein_id="YP_010806426.1"
misc_feature 8555..9757
/locus_tag="QJ817_gp2"
/note="Alphavirus E2 glycoprotein; Region:
Alpha_E2_glycop; pfam00943"
/db_xref="CDD:425959"
mat_peptide 9776..9946
/locus_tag="QJ817_gp2"
/product="6K"
/protein_id="YP_010806427.1"
misc_feature 9800..11260
/locus_tag="QJ817_gp2"
/note="Alphavirus E1 glycoprotein; Region:
Alpha_E1_glycop; pfam01589"
/db_xref="CDD:279870"
mat_peptide 9947..11269
/locus_tag="QJ817_gp2"
/product="E1"
/protein_id="YP_010806428.1"
3'UTR 11273..11604
ORIGIN
1 atagggtatg gtgtagaggc agccacccga cctatcctat ccaaaatgga gaaagtacac
61 gtggacttag acgctgacag cccttacgtc aagttactgc agaagtgctt tccgcatttt
121 gagatagaag ccacgcaggt caccgacaat gaccatgcca atgctagagc gttttcgcat
181 ctagccacca aactcatcga aagcgaagtg gacccggacc aggttatcct ggatattgga
241 agcgcgcctg tgcggcacac gcattccaag cacaagtacc actgtatctg cccaatgatc
301 agcgcggaag accctgatcg actccaaagg tatgcagata agctgagaaa gagtgatgta
361 acagataggt ttatagcctc caaagctgct gacctactga cggtgatgtc aacaccagac
421 gtagagactc catcattatg catgcacaca gattccactt gcaggtacca cggtactgtt
481 gccgtatacc aagatgtata cgcagtgcat gcacctacct ctatttacca ccaagcactg
541 aaaggtgtgc gcaccatcta ctggataggg tttgatacca ccccattcat gtacaagaat
601 atggcaggtg cgtacccgac ttataacact aactgggccg atgaaagtgt gctagaggca
661 aggaacattg gtctgtgcag ctcagacctg catgaaaaac gacttggaaa gatctccatc
721 atgaggaaaa agaaattgca acctactaac aaagttgtgt tttctgtagg ttcaacaata
781 tacactgaag aaagaatact tttgcgcagc tggcatctgc cgaacgtttt ccatcttaaa
841 gggaagacga gttttacagg cagatgcaat actatcgtta gctgcgatgg ttacgttgta
901 aagaagatta ccatcagtcc cggtatctac gggaaggttg ataatctggc ttctaccttg
961 caccgcgagg gtttcttgag ctgcaaggtg acagatacgt tgcgagggga gagggtatcc
1021 tttcccgtgt gcacgtatgt accggcaaca ctgtgcgatc agatgacagg gattctggct
1081 actgatgtca gtgttgacga cgctcagaaa ctgctggttg ggctcaacca gcgcatcgtc
1141 gtcaatggga gaacacaacg taacacaaat actatgcaaa attacctttt gccagtagta
1201 gcccaagcgt tctccaggtg ggcgcgtgaa tatcgcgctg acttggagga cgaaaaagac
1261 ctaggggtgc gggaacgctc cctagtcatg ggctgctgct gggccttcaa gactcataaa
1321 atcacatcca tctacaagaa acctggtacc caaactatca agaaggtacc agcggtcttt
1381 aactccttcg tcatccccca attcaacagc tacgggttga atatagggtt acgccgcaga
1441 attaaaatgc tcctggaaga gaagaggaag ccggccccta ttatcaccga agccgacgtt
1501 gcccatctta aggggatgca agaagaggca gaggcggtag cagaagctga agctgtgaga
1561 gcagctttac cgccactttt gcctgaagta gagagagaga ccattgaagc agacatagat
1621 ctgataatgc aagaagcagg tgcgggaagt gtcgagacgc ccagacggca cataaaagtc
1681 acaacatatc caggtgaaga gacgatcgga tcttatgcag tgctctcgcc acaggctgtc
1741 cttaacagcg agaaattagc ctgcatccac ccgctggctg agcaggtgct cgtcatgacc
1801 cataaagggc gtgcaggtag gtataaagtc gaaccctacc atggacgggt agttgtacct
1861 agtggtactg ccattccaat tccagacttc caagcgctga gcgagagtgc aaccatcgtc
1921 tacaatgaac gggaatttgt caatcggtat ttacaccata ttgccatcaa tggaggtgcg
1981 ataaacaccg acgaagagta ctacaaagtt cttagaagta gcgaggcgga ttcggagtac
2041 gtcttcgata ttgacgccag aaagtgcgtc aaaaaggctg acgcaggacc gatgtgcctt
2101 gttggtgagc tggttgaccc accctttcac gagtttgcat acgaaagtct gaaaacccgt
2161 ccagcggcgc cccataaggt gccgaccatt ggagtctacg gagttcctgg ctcaggaaaa
2221 tcgggaatca tcaagagcgc agtcacgaaa agagacctag tggtcagcgc aaagaaggag
2281 aactgcacgg aaattatcaa agatgttaaa cgtatgcgag gcatggatat tgcagcccgt
2341 acggtggact ccgtgttgct taacggggtc aaacacccgg tagacacact gtacattgac
2401 gaggcctttg catgccacgc ggggacgtta ctagcactca ttgccattgt caaaccaaag
2461 aaagtagtat tgtgtggaga cccgaaacag tgtggattct ttaacatgat gtgtctaaaa
2521 gtgcatttca accatgaaat atgcaccgaa gtgtaccaca agagtatctc ccgcaggtgc
2581 accaagacag tcacagctat cgtctctaca ttattctatg acaagagaat gagaacggtg
2641 aatccgtgca gtgacaagat cattatagac accaccagca caacaaaacc acaacgagat
2701 gacattatac tgacatgctt tagagggtgg gttaagcaac tacaaattga ctataaaaac
2761 cacgagatca tgacagcggc cgcatctcaa ggattaaccc gcaaaggtgt gtacgcagtc
2821 agatacaaag tcaatgaaaa tcccctctat gcacagacat ctgaacacgt aaatgtcctc
2881 ttgacccgca ccgagaaacg cattgtttgg aaaactcttg ccggggaccc ttggataaag
2941 accctaacgg ctcactaccc tggggaattt tcggcaacat tggaggagtg gcaagccgag
3001 catgatgcca ttatggagcg tatcctggaa actccagcta gtagcgatgt ataccagaat
3061 aaagtgcatg tatgctgggc aaaagcactt gaaccagttt tggccactgc caatataaca
3121 ctcactcgtt cccaatggga gaccatccct gcctttaaag acgacaaggc tttctccccg
3181 gaaatggcgt tgaacttctt gtgcaccagg ttcttcgggg ttgacattga cagtggatta
3241 ttctcagcgc caactgtacc tttaacatac acaaatgaac attgggataa cagccctggc
3301 ccgaacagat acggcttgtg catgcgtact gccaaagaac tggcccgtcg ctacccatgc
3361 atactgaaag ctgtagatac aggccgcctt gcggatgtgc gtaccaatac catcaaagac
3421 tacagccccc tgatcaacgt ggtgccactc aaccgtcgtc ttccacactc attggtcgtg
3481 tcgcacagat ataccggtga cggaaattat tctcagctgc tgtcaaagtt gataggaaaa
3541 accgtgctgg taataggaac cccaatttcc gttcctggaa aacgtgttga gacactaggc
3601 cctggtccac agtgcacata caaggcagac ttggacttag gcatccccag cacgattgga
3661 aaatatgaca taatatttgt caacgtcagg acaccgtaca agcaccacca ttatcagcag
3721 tgcgaagacc atgccattca ccacagcatg cttactagaa aggccgttga tcacctgaac
3781 aagggaggca cttgcgtggc actaggctac ggcaccgcag atagagcgac cgaaaatatt
3841 atttcagcgg tagctcgatc ttttagattc tcgagagtct gtcagcctaa gtgtgcgtgg
3901 gaaaacactg aagtcgcgtt tgtctttttt ggcaaggaca atggtaacca cctacgcgac
3961 caggatcaac taagtattgt actgaataac atctatcaag gttctaccca gtacgaagcg
4021 ggcagagcac cagcttacag agtgattcgg ggagacatat ctaagagcac cgacgaagcc
4081 attgttaatg cagctaacaa taaaggacaa ccgggagccg gagtatgtgg agcattgtat
4141 aagaaatggc caggggcttt tgataaggtg cccattgcga caggtactgc gcacctagtt
4201 aagcatacgc ccaacattat ccatgcagtc gggccgaatt tctcccgcgt atccgaagtg
4261 gaaggcaatc agaaactatc ggaggtctac atggacatcg caaaaatcat taacagggaa
4321 cgatacaata aggtgtcaat acctctgtta tcaactggca tttacgcagg tggcaaggat
4381 cgcgtgatgc aatcgttaaa ccacctgttt actgccatgg acaccaccga tgcagatgta
4441 actatctatt gcctggataa acagtgggaa gcacgaatca aagacgccat cgcccggaag
4501 gaaagcgtcg aggaactcgt agaagatgac aaacctgtcg acatcgagtt ggtccgtgtg
4561 cacccactaa gtagtctggt cgggagacca ggttactcta cggacgaagg caaggtgcac
4621 tcctatctgg aaggaacgag gttccaccaa actgccaaag acatcgcaga gatctacgct
4681 atgtggccta ataaacaaga agctaacgag caaatctgtc tgtacgtatt aggagaaagc
4741 atgaccagta tccgctctaa gtgccctgta gaagattccg aagcctcatc tccaccgcac
4801 acgattccgt gtctttgcaa ttatgccatg acggccgagc gggtttacag attacgcatg
4861 gcaaagaatg agcagtttgc cgtgtgttca tcatttcaat tacccaagta cagaataacg
4921 ggcgtgcaga agatccagtg caataagcca gtcatattct cgggggtcgt accaccagca
4981 atacatccac ggaaattctc ggctatcgag gaaacagtgc cagtaacaat tgaacggctt
5041 gtcccaagac gtcctgctcc accggtgccg gtacctgccc gtattcctag cccacgctgc
5101 tcaccagcag ttagcatgca gtctttgggc ggtagtagta catcggacgt tgtcatctcc
5161 gaagccgaag ttcatgattc agattccgat tgcagtatcc ctccaatgcc tttcgtcgtg
5221 gaggcagagg ttcacgcaag tcagggctca cactggagca ttcctagcgc gtctggcttc
5281 gaaatccgtg aactgccgga agatcgcagc atatccggct caccgacacg cacgtcggta
5341 atttccgacc actccgtgaa cctgatcaca tttgacagcg ttactgacat atttgaaaac
5401 ttcaaacaag cgccttttca gtttctatca gagatccgcc caataccagc accgcgtcgc
5461 agagtgggag ggtttgagac tgatactaag cgctacgaca agacagagga aaaaccaatt
5521 cctaagccac gtacgcgcac aacgaagtat aagcagcccc caggtgtagc caggtccatt
5581 tcggaagcag aattggatga gttcatccgc cgacattcaa attgacggta tgaagcgggt
5641 gcgtatattt tctcgtcaga gacagggcaa gggcatctgc aacagaaatc aactcggcag
5701 tgcaaactcc aaaatcctat cttggagcgc tctgtccacg agaaattcta cgccccgcgc
5761 ctcgacttgg aacgtgagaa actgttacag aaaaaattgc aattgtgcgc gtcggaaggt
5821 aataggagta ggtaccaatc acgtaaagtc gagaatatga aagcaattac ggcggaacgt
5881 ctgttgcaag ggataggggc ttatctttca gctgaatcgc agccagtgga gtgttataaa
5941 gttaattacc ccgtccccat ttactccacc acacgcagca acagattctc atcggcagac
6001 gtcgcggtaa gagtatgtaa tttggtattg caagagaact ttcctactgt ggcgagctac
6061 accatcaccg atgagtacga cgcctattta gatatggttg acggagcatc gtgctgcttg
6121 gatactgcga ccttctgccc ggctaagcta cgcagtttcc cgaaaaagca tagctacttg
6181 cgtcctgaga taagatcagc tgttccgtcc cccatacaga acacacttca gaacgtttta
6241 gcggcagcca ccaaacgaaa ctgtaacgtc acccagatgc gcgagttacc agtcctggac
6301 tcggccgcat ttaatgtgga gtgctttaaa aaatatgcat gtaacgacga gtactgggat
6361 acatttaaaa ataaccccat taggcttact actgagaatg tcactcagta cgttactaag
6421 ctcaaaggac caaaagctgc tgcactgttc gctaagacgc ataacttaca accactgcat
6481 gaaattccga tggatagatt tgtgatggat cttaagcgcg acgtcaaagt aactccgggg
6541 accaagcata ccgaagaacg accgaaagtc caagtgatac aggcggcgga acccctggcg
6601 accgcatatt tgtgcggaat acatagggag ttggtaagaa gactgaacgc cgtactgctg
6661 cctaacattc acacgttgtt cgatatgtcc gctgaggact ttgatgccat catagccgaa
6721 catttccaat tcggagacgc agtattggaa acagatatcg cttcgttcga caaaagtgag
6781 gatgatgcca ttgctatgtc tgcacttatg atccttgaag acctgggtgt cgatcaggcc
6841 ctcctagatc ttatagaagc tgcttttggg aatatcacat ctgtccacct cccaacaggg
6901 acacgcttca agttcggagc tatgatgaag tcaggtatgt tcttaaccct gtttattaat
6961 actgtggtta atattatgat tgccagccgc gtgttgcgtg agaggctaac caattcgcct
7021 tgcgctgctt ttattggcga tgataatatt gtgaaaggag tcaagtctga cgcactcatg
7081 gctgaacgtt gtgccacttg gttgaatatg gaagtaaaga taatagatgc aaccgtgggt
7141 gtaaaagctc cgtatttctg cggcggcttt atcgtcgtgg accaagtgac tggtacagca
7201 tgcagagtgg cagaccccct taagaggctg tttaaacttg gtaagccttt gcccttggac
7261 gacgatcagg atggggatag gcgcagagca ttgtacgacg aagcgctcag atggaaccgg
7321 atcggtatca ctgatgagct aatcaaagcc gttgaatcac gatatgaagt gttttacatc
7381 tcattggtta tcaccgcttt gactactctt gcagctacag tcagcaattt caaatatata
7441 agaggaaatc ctataaccct ctacggctga cctaaatagg ttgtgcagaa gaagctaacc
7501 tattatacaa cactcagtgt atcacaagcg caataccgaa acgatgtttc cgtatccaac
7561 attgaactac ccgcctatgg caccggttaa tccgatggca tacagggacc ccaatccacc
7621 aagacgtagg tggcggccat ttcggccgcc cctagctgct caaatcgagg atttgagacg
7681 ttccatcgct aacctaacgt ttaagcaaag ggccccgaat ccgccagcag gccccccggc
7741 aaaacgtaag aagccagcac ctaaaccgaa gcctgcggcg cccaagaaaa agaggcaacc
7801 acctccagct aagaagcaga agcgcaaaca aaagcctggt aaacggcaaa ggatgtgtat
7861 gaaactggaa tccgacaaga ctttccccat catgttgaat ggtcaagtga acggctatgc
7921 gtgcgtagtc ggcgggcgcg tgttcaaacc gctgcatgtt gaaggcaaga ttgacaacga
7981 acagttggcc gccattaaat tgaagaaagc aagtatctat gatctcgagt atggcgatgt
8041 tccacaatgt atgaaatcgg acaccctcca gtacaccagt gagaagccgc cgggtttcta
8101 caattggcac cacggagccg tgcagtatga aaacaacagg ttcaccgtgc cacgaggagt
8161 gggcggaaaa ggggatagcg gacgcccgat tttagacaac cgcggccgtg tggtcgctat
8221 agtactaggc ggagctaacg aaggatctcg cacagcgctg tcagtggtca cgtggaacca
8281 gaaaggggtt accgtaaagg atacccccga aggttccgaa ccttggtcac tgactacagt
8341 tatgtgcgtc ttagctaaca tcaccttccc atgtgaccag ccaccctgta tgccctgctg
8401 ttacgagaaa aatccgcacg agacgctcag catgctcgaa cagaactatg acagccaggc
8461 ttatgaccta ctgctggacg cggccgtcaa atgtaacggc aggagaaccc gtagagacct
8521 ggaaacccat ttcacacagt acaagctggc tcgcccatac atagcagact gctccaattg
8581 cggccacggc agatgcgaca gtcccattgc catcgaggac attcgcggag acgctcatgc
8641 aggctacatc cgaatacaaa catcggcaat gttcggcctg aagtcggatg gagtagatct
8701 agcctatatg agcttcatga acggcaaaac cctgaaggcc ataaaaatag aacatttgta
8761 tgctcgtaca tcagcaccat gctctctggt ttcctaccat ggttactaca tccttgccca
8821 gtgtcctccc ggagacactg tgacagtggg gtttcaagac ggtgccatca agcacatgtg
8881 caccatcgcc cataaagttg agttcaaacc tgtaggaaga gagaagtacc gccatcctcc
8941 tgaacacggc gtggagttac cctgcaccaa gtacacgcac aaacgtgcag accagggcca
9001 ctatgtcgaa atgcaccaac cagggctggt agccgaccac tcgctactga gcatgagcag
9061 tacgaaagtg aagatcaccg tcccaagtgg ttctcaagtg aagtattact gtaagtgccc
9121 cgacgtaaag gagggaacaa ccggcagcga ctacacgact gcttgcacgg acttgaagca
9181 gtgtcgagcg taccttattg acaataagaa atgggtctac aactctggca aactacctag
9241 aggagaaggc gaaaccttta aaggtaaact ccatgtgccg ttcgtcccgg tcacgagcaa
9301 gtgtaccgcg accttggccc ccgaaccact ggtggaacac aagcatcgat ccctagtttt
9361 acacctgcac ccagaacacc caacactact gacgactaga gcactgggaa gtaacgcgag
9421 gccgacaagg cagtggatcg aacaaccaac cacagtcaat tttacagtaa caggagaagg
9481 ttttgaatac acctggggca atcacccgcc caaacgagtt tgggcccaag aatcaggtga
9541 aggaaatcct catggttggc cgcacgaaat agtaatttat tactacaacc ggtatccaat
9601 gacgactgtc attggactgt gcacgtgcgt agccatcatt atggtttcct gtgttacatc
9661 tgtatggctt ctttgccgca ctcgcaatct ttgcataaca ccatacagat tggcacctaa
9721 cgcccaggta cccatcttgt tagcagtcct atgctgcgtg aaaccaacca gagcagatga
9781 tactttgcag gtcctaaatt atctgtggaa taacaaccag aacttctttt ggatgcagac
9841 ccttatacca ctggcagctt tgatagtgtg catgcgcatg ttgcgctgct tgctatgctg
9901 cggtccggct tttttacttg tctgcggcgc cttgggcgcc gcagcatacg aacacgcagc
9961 agtgatgccg aacaaggtgg ggatccccta caaagcgctg gttgaacgcc cgggctatgc
10021 accagtccac ctgcaaattc aattggtaac tactaaaatc atcccgtcag cgaatctgga
10081 atatatcacc tgcaagtata aaaccaaggt gccctctcct gtagtcaaat gctgcggctc
10141 cacccagtgc tcagcaaaat cactccctga ctaccaatgt caagtgttca caggggtcta
10201 cccatttatg tggggaggag cctattgctt ctgcgatacc gaaaatacgc agatgagcga
10261 agtgtacatt gaacgcgccg aggaatgttc agtcgaccag gctaaggcat acaaggtgca
10321 cactggcaca gtgcaggcgg tggtcaacat cacctacggg agcgtcagct ggcgatcggc
10381 tgacgtttac gtcaacggtg aaacacctgc aaagatcggc gatgccaaat taactatagg
10441 acctttgtcc tccgcctggt ctccgttcga ctcaaaggtt gtagtgtatg ggcacgaggt
10501 gtataattat gactttccag aatacggcac aggaaaagcc ggttcttttg gtgatctgca
10561 atccagaaca ctcaccagta acgacttgta cgccaacaca aacctgaagt tgcaacgacc
10621 acaaccagga gtagtccata cgccttatac ccaggcgcca tccgggttcg aacgctggaa
10681 gaaagaccgg ggagctcctt tgaatgacat cgctcccttt gggtgcacga tagctttaga
10741 cccactgcga gcggagaact gcgctgtggg caacatcccc ctttctatag acatcccgga
10801 tgccgcattt accaggatcg cagagacgcc cactgtgtcc gacctggaat gcaaggttac
10861 tgaatgcaca tatgcatccg acttcggggg aattgccact atcagctata aggcaagcaa
10921 agcaggtaac tgccccattc actccccgtc aggcattgca gtaatcaaag aaaacgatgt
10981 tacactggca gacagcggtg ccttcacgtt tcacttttcc acagccagca tccacccggc
11041 atttaaaatg caggtgtgta ctagcgtagt tacctgcaaa ggtgactgta agccaccaaa
11101 ggaccacatt gtcgattacc cggcccaaca tacagaaacg tacacatcag cagtatcggc
11161 aaccgcttgg tcatggctga aagtgctagt gggaagcaca tcagcattta tcgtgctggg
11221 gttgattgct actgcagtgg tcgccctggt actatttacc cacagacact aacgtactat
11281 catatcatat atcatcatgg ttcgacgtac ttccgagcca cgatgacggt ggtgcataat
11341 gccacctgcg cagtgcataa tgctgcgact tataggtagt acgctaccct ttataacact
11401 actggcagtg cataatgctg ccttttataa aaacactact ggcggcgcat aatgctgcct
11461 tttataacac tactggcagt tacaacgctg ccttttataa acttttacaa cactactggc
11521 ggcgcataat gctgcctttt ataaaatctt taaaattcat atacaatttt ttcttttatg
11581 tttttatttt gtttttaata tttc
//
