LOCUS NC_078660 6393 bp RNA linear ENV 07-MAY-2023
DEFINITION MAG: Miglotas virus isolate CMS002_047i_WVAL RNA-dependent RNA
polymerase (QK753_gp1) gene, complete cds.
ACCESSION NC_078660
VERSION NC_078660.1
DBLINK BioProject: PRJNA485481
BioSample: SAMN14051580
Sequence Read Archive: SRR11035332
KEYWORDS ENV; Metagenome Assembled Genome; MAG; RefSeq.
SOURCE Miglotas virus (mosquito metagenome)
ORGANISM Miglotas virus
Viruses; Riboviria; Orthornavirae; Negarnaviricota;
Polyploviricotina; Ellioviricetes; Bunyavirales; Phasmaviridae;
Orthophasmavirus; Orthophasmavirus miglotasense.
REFERENCE 1 (bases 1 to 6393)
AUTHORS Batson,J., Dudas,G., Haas-Stapleton,E., Kistler,A.L., Li,L.M.,
Logan,P., Ratnasiri,K. and Retallack,H.
TITLE Single mosquito metatranscriptomics identifies vectors, emerging
pathogens and reservoirs in one assay
JOURNAL Elife 10, e68353 (2021)
PUBMED 33904402
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 6393)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (05-MAY-2023) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 6393)
AUTHORS Batson,J., Dudas,G., Haas-Stapleton,E., Kistler,A.L., Li,L.M.,
Logan,P., Ratnasiri,K. and Retallack,H.
TITLE Direct Submission
JOURNAL Submitted (10-DEC-2020) Infectious Disease Initiative, CZ Biohub,
499 Illinois St, San Francisco, CA 94158, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to MW434703.
##Assembly-Data-START##
Assembly Method :: IDSeq pipeline v. 3.2
Coverage :: 18.741925x
Sequencing Technology :: Illumina
##Assembly-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..6393
/organism="Miglotas virus"
/mol_type="genomic RNA"
/isolate="CMS002_047i_WVAL"
/host="Culex erythrothorax"
/db_xref="taxon:2800927"
/environmental_sample
/country="USA: West Valley, California"
/lat_lon="33.93778 N 117.633056 W"
/collection_date="29-Nov-2017"
/metagenome_source="mosquito metagenome"
/metagenomic
/note="unknown sex, unfed, collected in marsh/swamp
habitat"
gene 32..6304
/locus_tag="QK753_s1gp1"
/db_xref="GeneID:80557555"
CDS 32..6304
/locus_tag="QK753_s1gp1"
/codon_start=1
/product="RNA-dependent RNA polymerase"
/protein_id="YP_010840370.1"
/db_xref="GeneID:80557555"
/translation="MEQLFKHVGKADRHDPKTQHTPASRAADMDTANGIPKQTIVQIA
RDLQNADELSPESAYSFVHSLTEARHDLWYEAIYSNLHNRWRTLGAETKVHDFFRLVL
KIDNVPARMFNQTPDIMLYDPESNEVALGDIAVTRAEANVTAIKHEKYLHVKEEILKY
GYAVIHEDFIVREDLANIRRLIQRYKNMTILAVSFNGSSTIRFTETATHLITDCLRKC
TDMTVYHKIVEKSQRTKEEKMNVHEFFDPEYMSKVPDYTPHRTEAELIQMIKDKTDEL
GGARYYDSDMDMINKAFDGIIESNRGKRVMAPKSVLKICDNSHDYTETTDHLLISSYI
EDLIQDDSSDVRDYIISMLPTMPQVKLMKDMPSNFKRSDLKSSKEHKMAGVGGRYQYK
RRRMYTNPICVEFETQITKGSGKKCTPNIKKEPRTIDMDQLNVFKSLTDIQINYYGSK
SNKPTFLSDDWDSYNKNEEDNTRQDRQVYNYVKATNGAQLCHSLSGLFSRLTHMSTFQ
GSYDNVYCPPNGSFIAMIPSEHAPVNSSNCDMPFIFITRTKLGCPLDHVEYEHRYVGS
NYIYYISKLCRLNVKKISCWENAGYRLVASASYLLTKCEALMSIKEKLVGMLTMMVID
IHQKVSEYLDLLKYISFMPFSELNMLPKLISDKCNLLMKTRMDAWMFQQLRSFVIELG
KTDKLEAEKPVLQLHNGKITKDSLGISVKLPSFIDQNVRHNKPSEFIEEISMIYTSRP
KHLYGSQFLDESTTQTAKWNLEFDDEKEKFLGWASGHDVGPYPFDAKFCYSSDVIHYA
TLELMKTMSATSIKMENDLYRSQYLDYLHTNCSLRGCTKDPEQRSSSTDIHTTSIDAC
FKFYKKINYSEKESRAVSVAQSFLASGEKMEFSMSEKDQRGGGRPIATPTLGAKALLM
AVEKPEAVMGKYMPNNIIVAGKNKLKEQYEAYTSCASDGARSGLTKIYQLTEDQTKYS
ENDNPTKYIPYIKTNTTMPDHVRNIQIAGVNSMMTRHHLVKRLPKEINETPELRKEVI
QDNLRNGVVARIGWPQGMYNYISTSVHCAADIWITKAYKIAYPNDKILTRGLVHSDDS
WVTVCCDHVDTFKRFSLFRTLAKKMFCLKINEKKLWGGKYLGELVSNYNLNGNVHLST
GKVIANGLSNLTYQNWPIDVSNQISTLQQAYRQGATLGNLILLSTVLRQQIFSSYTIT
GYQRAHLHHLPIELGGYPSCSVFSLAVCGVHAHSSDIHRMMTTVATDDTQGPIDIITA
ACYMSITKHETPGYTADLVDAADFLAIELPDRGEVFSAVRHLMPKSRKISKALETIYE
TVANFPSDGLALIVTRPNTLAESLGHYGELVSNKLFSLASEHYTQSARRLAISQTMQS
KGKVVRIGENPPMTFNEAYVVLLRTASPNAMKATAINAFKPHNDVVTACDIIVRTATL
EATDKKKGGVINRMPEIENAFRTISPMQDVLLYIIDDKTGTKHFMKYSSDKTSITTVK
NDMNAIISRFKHYFSYFEPKQACQLIMQAKMSTMKERAWVQPKVNTDTLSCFLEDLYG
KTINSSQNYKVRSDRGYNTILTESADVVQTIYGCLVLNSIYYKKFQILKFKDKDVEDV
LDDIDPANLDNNSKLKLATCQFLVNNNREYLDNFVKNEKFASKWIRTQRHHNGRYSGD
WEAVFMCGSTVCSVRSINTIVTITVNKLDLRHILKGMRLFVAKCFSQHSYEYDSAWWT
SRAWNSNTQPDPNATAYLCSFNSYNTSIRSEPSERYINIKVNPNLQFSDITDFQLPDD
YSISDGLRVVNAITHHVDGTETSHRVGNVFQSFSIPFYEHVDLRHEYIDGFSNIDLLK
SRVIEDITLFRPLSISKSDLIKLLDKCVELPMASTLWNTFVNIDRKTVGVNRNIPPIV
DPGIPEIHVVEMDVETMSGAGVVDVAMTTDVESLESIATEYEVNKVRGAFLLHSPRNF
LRLLCSCLHPGIQQSHILDFIYGMVHDVNLFKWMRDIHDDVSIFYDISDMISQKEMYV
DYPKELFYFLYGNNMDLTKTWECVNRHVMTQRMRNMQRVSDYKPMLDCFVHALDDSFF
GNAVFVKPQTAFDMLQ"
ORIGIN
1 cagcacgcag agacataatt ttataaatag aatggaacaa ttattcaaac acgttggcaa
61 ggctgatagg catgacccta agacccagca tacgcctgca tccagagcag ccgacatgga
121 cacagctaat ggtatcccaa aacagaccat agttcaaatc gcaagagatc tccagaatgc
181 agatgaactg agccctgagt cagcatacag ctttgttcac tctttaactg aagcacgaca
241 cgacctctgg tatgaggcta tttattccaa tctacataat cgatggagga cacttggtgc
301 agaaactaaa gtgcatgact ttttccgtct ggttttgaaa attgacaacg tgccagccag
361 gatgttcaat caaacaccag acataatgct atatgatcca gaaagcaatg aggttgccct
421 cggtgatatt gctgttactc gtgctgaggc aaacgttaca gctattaagc atgaaaaata
481 tctgcatgtg aaggaggaaa tactgaagta tggctatgca gttattcatg aagattttat
541 tgtacgtgaa gacctagcca acattaggag actgatacag cgctacaaga atatgactat
601 tttggctgtc tctttcaatg ggtcttcaac aatccggttc acagaaactg ccactcatct
661 tataactgac tgcttacgga aatgcacaga catgacagtt tatcacaaaa ttgtggagaa
721 atcgcaacgc acaaaggaag agaaaatgaa tgttcatgag ttttttgatc cagaatacat
781 gagcaaggtg cctgactaca caccacacag gacagaggca gaattgattc aaatgataaa
841 ggataaaaca gatgagctag gaggggcacg atattatgat agtgacatgg acatgataaa
901 caaggcattc gatgggataa ttgaaagtaa cagagggaag cgtgtgatgg caccaaagtc
961 cgttctgaag atctgtgata atagtcatga ttacactgaa accactgatc acctgcttat
1021 ctctagttac atagaggacc taattcaaga tgattcatca gatgtgcgtg actacatcat
1081 atccatgctc ccaactatgc ctcaagttaa gctaatgaag gacatgccca gcaatttcaa
1141 gcgctcagac ttgaagtcat caaaggaaca caaaatggca ggcgtcggtg gaaggtacca
1201 gtacaaacgg cgccggatgt acacaaaccc aatctgtgta gagttcgaaa cacagatcac
1261 taaaggatct gggaaaaagt gcaccccaaa tatcaagaag gagccacgga ccattgatat
1321 ggatcagctt aacgtcttca agtcactaac tgacattcaa attaattatt atggatcaaa
1381 atccaacaaa ccaacattcc tttcggatga ctgggatagc tacaacaaaa acgaagaaga
1441 caacacacgg caagataggc aagtgtacaa ctatgtcaaa gcaacaaatg gcgcacagct
1501 ttgtcactca ttaagtggac tcttctctcg gctaacacac atgtccactt ttcaggggtc
1561 ctatgacaat gtgtattgtc caccgaatgg atcttttatt gctatgattc caagtgagca
1621 tgcaccagtc aattcaagta actgtgatat gccatttatt ttcattacaa gaaccaagct
1681 cgggtgccca ctagatcatg tggaatatga acaccggtat gtaggatcca actacatcta
1741 ctacatctcc aagctctgca ggttgaatgt aaagaaaata agctgctggg aaaatgccgg
1801 atacaggcta gtagcatctg catcttatct gctaaccaag tgtgaagctc tcatgtctat
1861 caaagaaaaa ttggtgggca tgcttacaat gatggtgata gacatacatc aaaaagtttc
1921 agagtacttg gatctgctga agtacatatc atttatgccc ttttcagagc tcaacatgct
1981 cccaaagctg atatctgata agtgcaatct cctgatgaag acccgtatgg acgcatggat
2041 gtttcagcag ctgagaagtt tcgtcattga gctaggaaaa acagacaagc tagaagctga
2101 gaagcccgta ctgcagctac acaacggaaa gataacgaag gattcattag gcataagtgt
2161 caagctaccc agcttcatcg atcaaaatgt gcgtcacaac aagccatctg aatttataga
2221 agaaataagt atgatataca cttctaggcc aaaacacctt tatgggtcac agttcttaga
2281 tgagtcaacg acacagactg ccaaatggaa tcttgaattt gatgatgaga aagagaagtt
2341 cttgggctgg gcaagtggac acgatgttgg tccataccca ttcgatgcca agttctgcta
2401 ctcatctgat gttatacatt atgccacact ggagctcatg aagacaatga gtgccacctc
2461 aatcaagatg gagaatgatc tatatcgaag ccagtatcta gactacctcc acacgaattg
2521 ttcacttcgt ggatgcacaa aagatccaga acaacgatca tcttcaactg acattcacac
2581 aacatcaatt gatgcatgct tcaaatttta caaaaagata aactacagtg aaaaagaatc
2641 aagggcagta tctgtcgcac agtccttttt ggccagtggt gagaagatgg aattctcgat
2701 gtcagaaaag gaccagcggg ggggaggccg accaattgca acacccactc tgggtgcaaa
2761 agccctacta atggctgtgg agaaaccaga ggcagtaatg gggaagtaca tgccaaacaa
2821 tatcattgtg gctgggaaga acaagcttaa ggagcagtac gaggcataca cttcatgtgc
2881 atctgatgga gcaagatcag gactaacaaa aatttaccag ctgactgaag atcagactaa
2941 atattctgaa aatgacaatc caactaaata cataccttac attaaaacga acaccacaat
3001 gccagatcat gtcagaaaca tccaaattgc tggagtcaac agcatgatga cacgacatca
3061 tctcgtcaaa aggctgccca aagaaatcaa cgagacacca gaactaagga aggaagtcat
3121 acaagataac cttaggaacg gtgttgttgc acgtataggt tggccgcagg ggatgtacaa
3181 ctacatttca acaagtgttc attgtgctgc agacatttgg ataacaaaag cttacaagat
3241 agcatatcca aatgataaaa tattgacacg cgggctggtc cattctgatg actcatgggt
3301 cacagtctgc tgtgatcatg tagacacttt taagagattc tctttgttca gaactctagc
3361 aaagaagatg ttctgtctga agatcaatga aaagaagctg tggggaggga agtatctagg
3421 tgaactagta tctaactaca atttgaatgg gaatgttcac ctatctacag gaaaagtgat
3481 agcaaatgga ctctctaatc taacatatca aaactggcca attgatgtgt caaatcaaat
3541 atcaacttta cagcaggcat accggcaggg agcaaccctc ggaaacctga tactactatc
3601 cacagtgttg cgacaacaaa tattcagctc atacacaatt acaggatatc aacgagcaca
3661 cttgcatcat cttcccatag aattgggtgg ttaccccagc tgttctgtat tttcacttgc
3721 tgtgtgtgga gttcatgcac atagctcaga catacatcgt atgatgacaa ctgtcgctac
3781 agatgacact caaggtccaa tagatataat aactgctgca tgctacatga gcataaccaa
3841 gcatgaaaca cctggatata cagctgatct tgttgatgct gcagatttcc tagctataga
3901 attaccagat aggggagagg ttttctctgc tgtgaggcat ctcatgccaa agtctcggaa
3961 gataagtaaa gcactagaaa caatctatga gacagtggca aacttcccaa gtgatggact
4021 tgcgctaatt gtgacacgcc cgaacacact agcagaatca cttggtcact atggggagtt
4081 ggtatcaaac aagttgtttt cgttagcctc tgagcactac acacaatcag ccaggcgatt
4141 ggcaatctca cagactatgc aatcgaaagg taaggttgtt cgaattggag aaaaccctcc
4201 catgacgttc aatgaagcat atgtggttct gctaagaact gcatcaccga atgctatgaa
4261 agctacagca attaatgctt ttaaaccgca caatgatgtt gtcacagcat gtgacataat
4321 agttagaaca gccacgcttg aagcaactga taaaaagaag ggcggagtaa tcaatagaat
4381 gcctgagatc gaaaatgctt tcaggacaat atcaccaatg caagatgtgc tactgtatat
4441 aatagatgac aagaccggaa caaagcattt tatgaaatac tcgtcagata aaactagcat
4501 aaccacagtg aagaatgata tgaacgcaat tattagtaga tttaagcatt actttagcta
4561 ttttgaaccc aaacaggctt gtcagctaat tatgcaggca aaaatgtcta caatgaaaga
4621 aagagcatgg gtccagccaa aagtgaacac agatacactg tcttgctttc tcgaagatct
4681 gtatggcaaa acgatcaaca gcagtcagaa ttacaaggtt aggtctgaca gaggttacaa
4741 cacgatactg actgaatcag cagatgttgt ccagaccata tacgggtgtc tggtactcaa
4801 ttctatttat tacaaaaagt ttcagatttt aaaattcaag gataaagacg tggaagatgt
4861 cttggatgac attgacccgg ccaatcttga taacaactca aagctaaaac tggcaacttg
4921 ccaatttttg gttaacaata acagagaata cctagacaac ttcgttaaga acgagaagtt
4981 tgcatcaaag tggataagaa ctcagagaca tcacaatggc aggtactctg gggattggga
5041 agctgtgttt atgtgtggaa gcactgtttg ctcagtgcga tcaataaaca caatcgtcac
5101 aataacagtg aacaagttag atcttaggca cattctgaaa ggaatgaggt tgtttgtagc
5161 aaagtgtttc agccagcatt cgtatgaata tgattctgca tggtggacta gtcgtgcatg
5221 gaattccaac acccagcctg atccaaatgc tacagcttat ctgtgctcat ttaattcata
5281 taacaccagc atcagatctg agccatctga aagatatata aacatcaaag ttaaccctaa
5341 cctgcagttc tcagacataa ctgatttcca gttgcctgat gattacagca tttctgatgg
5401 gcttcgagtg gtgaatgcaa taacccatca tgttgacggc actgaaacaa gccaccgtgt
5461 tggcaatgtc ttccaaagct tcagcattcc attttatgaa catgtggatc tgcgacacga
5521 gtacatcgac gggttctcca acattgactt gctaaaaagc agagtgattg aggacataac
5581 gctgtttagg ccattaagca tttcaaagag tgacttgata aaattgcttg acaagtgtgt
5641 agaactgccg atggcatcta cattatggaa cacttttgtg aacattgaca ggaagacagt
5701 aggagtgaac agaaatattc cccctatagt tgatcctggg atcccggaga tacatgttgt
5761 cgagatggat gttgagacta tgtcaggtgc aggtgttgtt gatgttgcaa tgacaactga
5821 tgttgaatcc ctggagagta tagctacaga gtatgaggtc aataaggtgc gaggagcttt
5881 tttgcttcat tcacctagaa attttcttcg cttgctatgc agctgcctac atccaggtat
5941 ccaacaatct cacatattgg acttcattta tgggatggta cacgatgtca atctttttaa
6001 atggatgaga gatatacatg atgatgtaag tatcttttat gatatatcag acatgatatc
6061 tcaaaaagag atgtatgttg attaccctaa ggagctgttt tacttcttgt atggaaacaa
6121 catggatctc actaaaactt gggaatgtgt caatagacat gtcatgacac aaagaatgag
6181 aaacatgcag agagtgtccg attataagcc aatgcttgac tgcttcgtgc atgcattgga
6241 tgattctttc tttggaaatg ctgtctttgt aaagccgcag actgcttttg acatgttaca
6301 gtagataaaa caaagttact acctgataaa gaacggcttt tgcactttag ttatatatac
6361 tataaaaata tattttaata ttttgtagtc tca
//
