LOCUS NP_001092103 256 aa linear PRI 18-NOV-2023
DEFINITION proline-rich AKT1 substrate 1 isoform b [Homo sapiens].
ACCESSION NP_001092103
VERSION NP_001092103.1
DBSOURCE REFSEQ: accession NM_001098633.4
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 256)
AUTHORS Zhang T, Wang Y, Yu H, Zhang T, Guo L, Xu J, Wei X, Wang N, Wu Y,
Wang X and Huang L.
TITLE PGK1 represses autophagy-mediated cell death to promote the
proliferation of liver cancer cells by phosphorylating PRAS40
JOURNAL Cell Death Dis 13 (1), 68 (2022)
PUBMED 35058442
REMARK GeneRIF: PGK1 represses autophagy-mediated cell death to promote
the proliferation of liver cancer cells by phosphorylating PRAS40.
Publication Status: Online-Only
REFERENCE 2 (residues 1 to 256)
AUTHORS Kim K, Lee S, Kang H, Shin E, Kim HY, Youn H and Youn B.
TITLE Dual Specificity Kinase DYRK3 Promotes Aggressiveness of
Glioblastoma by Altering Mitochondrial Morphology and Function
JOURNAL Int J Mol Sci 22 (6), 2982 (2021)
PUBMED 33804169
REMARK GeneRIF: Dual Specificity Kinase DYRK3 Promotes Aggressiveness of
Glioblastoma by Altering Mitochondrial Morphology and Function.
Publication Status: Online-Only
REFERENCE 3 (residues 1 to 256)
AUTHORS Dougherty MI, Lehman CE, Spencer A, Mendez RE, David AP, Taniguchi
LE, Wulfkuhle J, Petricoin EF, Gioeli D and Jameson MJ.
TITLE PRAS40 Phosphorylation Correlates with Insulin-Like Growth Factor-1
Receptor-Induced Resistance to Epidermal Growth Factor Receptor
Inhibition in Head and Neck Cancer Cells
JOURNAL Mol Cancer Res 18 (9), 1392-1401 (2020)
PUBMED 32467173
REMARK GeneRIF: PRAS40 Phosphorylation Correlates with Insulin-Like Growth
Factor-1 Receptor-Induced Resistance to Epidermal Growth Factor
Receptor Inhibition in Head and Neck Cancer Cells.
REFERENCE 4 (residues 1 to 256)
AUTHORS Qi Z, Zhang T, Song L, Fu H, Luo H, Wu J, Zhao S, Zhang T, Guo L,
Jin L, Zhang H, Huang G, Ma T, Wu Y and Huang L.
TITLE PRAS40 hyperexpression promotes hepatocarcinogenesis
JOURNAL EBioMedicine 51, 102604 (2020)
PUBMED 31901857
REMARK GeneRIF: PRAS40 hyperexpression promotes hepatocarcinogenesis.
Erratum:[EBioMedicine. 2020 Oct;60:103001. PMID: 32979837]
REFERENCE 5 (residues 1 to 256)
AUTHORS Zhang H, Wei P, Lv W, Han X, Yang J, Qin S and Zhang Y.
TITLE MELK is Upregulated in Advanced Clear Cell Renal Cell Carcinoma and
Promotes Disease Progression by Phosphorylating PRAS40
JOURNAL Cell Transplant 28 (1_suppl), 37S-50S (2019)
PUBMED 31813279
REMARK GeneRIF: MELK is Upregulated in Advanced Clear Cell Renal Cell
Carcinoma and Promotes Disease Progression by Phosphorylating
PRAS40.
REFERENCE 6 (residues 1 to 256)
AUTHORS Huang B and Porter G.
TITLE Expression of proline-rich Akt-substrate PRAS40 in cell survival
pathway and carcinogenesis
JOURNAL Acta Pharmacol Sin 26 (10), 1253-1258 (2005)
PUBMED 16174443
REFERENCE 7 (residues 1 to 256)
AUTHORS Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S,
Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett
JR, Langeberg LK, Scott JD and Pawson T.
TITLE Proteomic, functional, and domain-based analysis of in vivo 14-3-3
binding proteins involved in cytoskeletal regulation and cellular
organization
JOURNAL Curr Biol 14 (16), 1436-1450 (2004)
PUBMED 15324660
REFERENCE 8 (residues 1 to 256)
AUTHORS Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li
J, Cohn MA, Cantley LC and Gygi SP.
TITLE Large-scale characterization of HeLa cell nuclear phosphoproteins
JOURNAL Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
PUBMED 15302935
REFERENCE 9 (residues 1 to 256)
AUTHORS Saito A, Narasimhan P, Hayashi T, Okuno S, Ferrand-Drake M and Chan
PH.
TITLE Neuroprotective role of a proline-rich Akt substrate in apoptotic
neuronal cell death after stroke: relationships with nerve growth
factor
JOURNAL J Neurosci 24 (7), 1584-1593 (2004)
PUBMED 14973226
REFERENCE 10 (residues 1 to 256)
AUTHORS Kovacina KS, Park GY, Bae SS, Guzzetta AW, Schaefer E, Birnbaum MJ
and Roth RA.
TITLE Identification of a proline-rich Akt substrate as a 14-3-3 binding
partner
JOURNAL J Biol Chem 278 (12), 10189-10194 (2003)
PUBMED 12524439
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
DA848626.1 and BC051844.2.
Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730)
that binds 14-3-3 protein (see YWHAH, MIM 113508) when
phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied
by OMIM, Mar 2008].
Transcript Variant: This variant (3) differs in the 5' UTR and
coding sequence compared to variant 1. The resulting isoform (b) is
shorter at the N-terminus compared to isoform a. Variants 2, 3, 4,
and 5 encode the same isoform (b).
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR14038196.2466897.1,
SRR11853565.15684.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA1965299,
SAMEA1966682 [ECO:0006172]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000344175.10/ ENSP00000341698.5
RefSeq Select criteria :: based on conservation, expression
##RefSeq-Attributes-END##
FEATURES Location/Qualifiers
source 1..256
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="19"
/map="19q13.33"
Protein 1..256
/product="proline-rich AKT1 substrate 1 isoform b"
/note="40 kDa proline-rich AKT substrate; AKT1 substrate 1
(proline rich)"
/calculated_mol_wt=27252
Site 51
/site_type="methylation"
/note="Omega-N-methylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Region 69..189
/region_name="Disordered.
/evidence=ECO:0000256|SAM:MobiDB-lite"
/note="propagated from UniProtKB/Swiss-Prot (Q96B36.1)"
Site 88
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:21406692,
ECO:0007744|PubMed:24275569; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Site 92
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:21406692,
ECO:0007744|PubMed:24275569; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Site 116
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q9D1F4; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Region 127..251
/region_name="PRAS"
/note="Proline-rich AKT1 substrate 1; pfam15798"
/db_xref="CDD:406279"
Region 129..133
/region_name="TOS motif.
/evidence=ECO:0000269|PubMed:17510057"
/note="propagated from UniProtKB/Swiss-Prot (Q96B36.1)"
Site 183
/site_type="phosphorylation"
/note="Phosphoserine, by MTOR.
/evidence=ECO:0000269|PubMed:17517883,
ECO:0000269|PubMed:18372248, ECO:0007744|PubMed:17081983,
ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332,
ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Region 197..216
/region_name="Disordered.
/evidence=ECO:0000256|SAM:MobiDB-lite"
/note="propagated from UniProtKB/Swiss-Prot (Q96B36.1)"
Site 202
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:24275569; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Site 203
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163,
ECO:0007744|PubMed:24275569; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Site 211
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:20068231; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Site 212
/site_type="phosphorylation"
/note="Phosphoserine, by MTOR.
/evidence=ECO:0000269|PubMed:18372248,
ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332,
ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (Q96B36.1)"
Site 221
/site_type="phosphorylation"
/note="Phosphoserine, by MTOR.
/evidence=ECO:0000269|PubMed:18372248; propagated from
UniProtKB/Swiss-Prot (Q96B36.1)"
Region 237..256
/region_name="Disordered.
/evidence=ECO:0000256|SAM:MobiDB-lite"
/note="propagated from UniProtKB/Swiss-Prot (Q96B36.1)"
Site 246
/site_type="phosphorylation"
/note="Phosphothreonine, by PKB/AKT1 and DYRK3.
/evidence=ECO:0000269|PubMed:12524439,
ECO:0000269|PubMed:23415227, ECO:0000269|PubMed:29634919,
ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332,
ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692;
propagated from UniProtKB/Swiss-Prot (Q96B36.1)"
CDS 1..256
/gene="AKT1S1"
/gene_synonym="Lobe; PRAS40"
/coded_by="NM_001098633.4:127..897"
/note="isoform b is encoded by transcript variant 3"
/db_xref="CCDS:CCDS12784.1"
/db_xref="GeneID:84335"
/db_xref="HGNC:HGNC:28426"
/db_xref="MIM:610221"
ORIGIN
1 masgrpeelw eavvgaaerf rartgtelvl ltaapppppr pgpcayaahg rgalaeaarr
61 clhdialahr aataarppap ppapqppspt pspprptlar edneededep tetetsgeql
121 gisdngglfv mdedatlqdl ppfcesdpes tddgslseet pagpptcsvp pasalptqqy
181 akslpvsvpv wgfkekrtea rssdeengpp sspdldriaa smralvlrea edtqvfgdlp
241 rprlntsdfq klkrky
//
