LOCUS NP_001247 824 aa linear PRI 04-JUL-2020
DEFINITION cell division cycle protein 27 homolog isoform 2 [Homo sapiens].
ACCESSION NP_001247
VERSION NP_001247.3
DBSOURCE REFSEQ: accession NM_001256.6
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 824)
AUTHORS Xin Y, Ning S, Zhang L and Cui M.
TITLE CDC27 Facilitates Gastric Cancer Cell Proliferation, Invasion and
Metastasis via Twist-Induced Epithelial-Mesenchymal Transition
JOURNAL Cell. Physiol. Biochem. 50 (2), 501-511 (2018)
PUBMED 30308498
REMARK GeneRIF: CDC27 facilitated cells invasion and metastasis via Twist
pathway, leading to initiation of epithelial-mesenchymal transition
and silencing of Twist expression could reverse this process.
REFERENCE 2 (residues 1 to 824)
AUTHORS Wang C, Su Z, Hou H, Li D, Pan Z, Tian W and Mo C.
TITLE Inhibition of Anaphase-Promoting Complex by Silence APC/C(Cdh1) to
Enhance Radiosensitivity of Nasopharyngeal Carcinoma Cells
JOURNAL J. Cell. Biochem. 118 (10), 3150-3157 (2017)
PUBMED 28004426
REMARK GeneRIF: The levels of CDC20 and CylinB1 increased and the levels
of Ku70 and APC3 decreased after irradiation. APC/C(Cdh1) is
involved in regulation of radiosensitivity in human NPC CNE-1
cells.
REFERENCE 3 (residues 1 to 824)
AUTHORS Yoshimura S, Kasamatsu A, Nakashima D, Iyoda M, Kasama H, Saito T,
Takahara T, Endo-Sakamoto Y, Shiiba M, Tanzawa H and Uzawa K.
TITLE UBE2S associated with OSCC proliferation by promotion of P21
degradation via the ubiquitin-proteasome system
JOURNAL Biochem. Biophys. Res. Commun. 485 (4), 820-825 (2017)
PUBMED 28257844
REMARK GeneRIF: Data indicate that UBE2S and APC3 co-regulate the
expression level of P21 at G2/M check point via the
ubiquitin-proteasome system.
REFERENCE 4 (residues 1 to 824)
AUTHORS Feng Z, Zhang L, Zhou J, Zhou S, Li L, Guo X, Feng G, Ma Z, Huang W
and Huang F.
TITLE mir-218-2 promotes glioblastomas growth, invasion and drug
resistance by targeting CDC27
JOURNAL Oncotarget 8 (4), 6304-6318 (2017)
PUBMED 27974673
REMARK GeneRIF: mir-218-2 promotes glioblastomas growth, invasion and drug
resistance by targeting CDC27 expression.
REFERENCE 5 (residues 1 to 824)
AUTHORS Yu H, Peters JM, King RW, Page AM, Hieter P and Kirschner MW.
TITLE Identification of a cullin homology region in a subunit of the
anaphase-promoting complex
JOURNAL Science 279 (5354), 1219-1222 (1998)
PUBMED 9469815
REFERENCE 6 (residues 1 to 824)
AUTHORS Ollendorff V and Donoghue DJ.
TITLE The serine/threonine phosphatase PP5 interacts with CDC16 and
CDC27, two tetratricopeptide repeat-containing subunits of the
anaphase-promoting complex
JOURNAL J. Biol. Chem. 272 (51), 32011-32018 (1997)
PUBMED 9405394
REFERENCE 7 (residues 1 to 824)
AUTHORS Ho PP, Couch FJ, Brody LC, Abel KJ, Boehnke M, Shearon TH,
Chandrasekharappa SC, Collins FS and Weber BL.
TITLE Localization of the human homolog of the yeast cell division
control 27 gene (CDC27) proximal to ITGB3 on human chromosome
17q21.3
JOURNAL Somat. Cell Mol. Genet. 21 (5), 351-355 (1995)
PUBMED 8619132
REFERENCE 8 (residues 1 to 824)
AUTHORS Tugendreich S, Tomkiel J, Earnshaw W and Hieter P.
TITLE CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic
spindle and is essential for the metaphase to anaphase transition
JOURNAL Cell 81 (2), 261-268 (1995)
PUBMED 7736578
REFERENCE 9 (residues 1 to 824)
AUTHORS Chen PL, Ueng YC, Durfee T, Chen KC, Yang-Feng T and Lee WH.
TITLE Identification of a human homologue of yeast nuc2 which interacts
with the retinoblastoma protein in a specific manner
JOURNAL Cell Growth Differ. 6 (2), 199-210 (1995)
PUBMED 7756179
REFERENCE 10 (residues 1 to 824)
AUTHORS Tugendreich S, Boguski MS, Seldin MS and Hieter P.
TITLE Linking yeast genetics to mammalian genomes: identification and
mapping of the human homolog of CDC27 via the expressed sequence
tag (EST) data base
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 90 (21), 10031-10035 (1993)
PUBMED 8234252
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from HY023336.1, S78234.1,
AC002558.2 and AA927469.1.
On Feb 8, 2008 this sequence version replaced NP_001247.2.
Summary: The protein encoded by this gene shares strong similarity
with Saccharomyces cerevisiae protein Cdc27, and the gene product
of Schizosaccharomyces pombe nuc 2. This protein is a component of
the anaphase-promoting complex (APC), which is composed of eight
protein subunits and is highly conserved in eukaryotic cells. This
complex catalyzes the formation of cyclin B-ubiquitin conjugate,
which is responsible for the ubiquitin-mediated proteolysis of
B-type cyclins. The protein encoded by this gene and three other
members of the APC complex contain tetratricopeptide (TPR) repeats,
which are important for protein-protein interactions. This protein
was shown to interact with mitotic checkpoint proteins including
Mad2, p55CDC and BUBR1, and it may thus be involved in controlling
the timing of mitosis. Alternative splicing of this gene results in
multiple transcript variants. Related pseudogenes have been
identified on chromosomes 2, 22 and Y. [provided by RefSeq, May
2014].
Transcript Variant: This variant (2) uses an alternate in-frame
splice site in the central coding region, compared to variant 1,
resulting in an isoform (2) that is shorter than isoform 1.
Sequence Note: This RefSeq record was created from transcript and
genomic sequence data to make the sequence consistent with the
reference genome assembly. The genomic coordinates used for the
transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1660807.224122.1,
SRR1803615.70207.1 [ECO:0000332]
RNAseq introns :: mixed/partial sample support
SAMEA1965299, SAMEA1966682
[ECO:0000350]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000066544.8/ ENSP00000066544.3
RefSeq Select criteria :: based on conservation, expression
##RefSeq-Attributes-END##
FEATURES Location/Qualifiers
source 1..824
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="17"
/map="17q21.32"
Protein 1..824
/product="cell division cycle protein 27 homolog isoform
2"
/note="anaphase promoting complex subunit 3; nuc2 homolog;
anaphase-promoting complex, protein 3; cell division cycle
27 homolog; D0S1430E, D17S978E"
/calculated_mol_wt=91737
Region 6..35
/region_name="TPR 1"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 17..92
/region_name="ANAPC3"
/note="Anaphase-promoting complex, cyclosome, subunit 3;
pfam12895"
/db_xref="CDD:289650"
Region 38..65
/region_name="TPR 2"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 67..99
/region_name="TPR 3"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 115..145
/region_name="TPR 4"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Site 205
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000244|PubMed:18669648,
ECO:0000244|PubMed:23186163, ECO:0000269|PubMed:14657031;
propagated from UniProtKB/Swiss-Prot (P30260.2)"
Site 209
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000269|PubMed:14657031; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 244
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000269|PubMed:14657031; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 291
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000269|PubMed:14657031; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 313
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000269|PubMed:14657031; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 339
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000244|PubMed:18669648; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 366
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000244|PubMed:19690332; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 379
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000244|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 386
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000244|PubMed:20068231; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 426
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000244|PubMed:18669648,
ECO:0000244|PubMed:23186163, ECO:0000269|PubMed:14657031;
propagated from UniProtKB/Swiss-Prot (P30260.2)"
Site 430
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000269|PubMed:14657031; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 435
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000269|PubMed:14657031; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 438
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000244|PubMed:18669648; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
Site 446
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000244|PubMed:18669648,
ECO:0000244|PubMed:23186163, ECO:0000269|PubMed:14657031;
propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 457..528
/region_name="TPR_12"
/note="Tetratricopeptide repeat; pfam13424"
/db_xref="CDD:290160"
Region 465..495
/region_name="TPR 5"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 499..528
/region_name="TPR 6"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 501..762
/region_name="TPR"
/note="Tetratricopeptide (TPR) repeat [General function
prediction only]; COG0457"
/db_xref="CDD:223533"
Region 501..527
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 533..563
/region_name="TPR 7"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 567..600
/region_name="TPR_1"
/note="Tetratricopeptide repeat; pfam00515"
/db_xref="CDD:278916"
Region 567..598
/region_name="TPR 8"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 567..595
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Site order(568,571..572,575..576,578,602,605..606,609..610,
612..613,636,639..640,643..644,647)
/site_type="other"
/note="putative protein binding surface [polypeptide
binding]"
/db_xref="CDD:276809"
Region 600..630
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 601..634
/region_name="TPR_1"
/note="Tetratricopeptide repeat; pfam00515"
/db_xref="CDD:278916"
Region 601..631
/region_name="TPR 9"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 635..667
/region_name="TPR 10"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 635..667
/region_name="TPR_1"
/note="Tetratricopeptide repeat; pfam00515"
/db_xref="CDD:278916"
Region 635..663
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 669..696
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 670..702
/region_name="TPR 11"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 672..702
/region_name="TPR_1"
/note="Tetratricopeptide repeat; pfam00515"
/db_xref="CDD:278916"
Region 703..731
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 704..734
/region_name="TPR 12"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 737..768
/region_name="TPR 13"
/note="propagated from UniProtKB/Swiss-Prot (P30260.2)"
Region 737..765
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Site 821
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000269|PubMed:22139841; propagated from
UniProtKB/Swiss-Prot (P30260.2)"
CDS 1..824
/gene="CDC27"
/gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
H-NUC; HNUC; NUC2"
/coded_by="NM_001256.6:124..2598"
/note="isoform 2 is encoded by transcript variant 2"
/db_xref="CCDS:CCDS11509.1"
/db_xref="GeneID:996"
/db_xref="HGNC:HGNC:1728"
/db_xref="MIM:116946"
ORIGIN
1 mtvlqepvqa aiwqalnhya yrdavflaer lyaevhseea lfllatcyyr sgkaykayrl
61 lkghscttpq ckyllakccv dlsklaegeq ilsggvfnkq kshddivtef gdsacftlsl
121 lghvycktdr lakgsecyqk slslnpflws pfeslceige kpdpdqtfkf tslqnfsncl
181 pnscttqvpn hslshrqpet vltetpqdti elnrlnless nskyslntds svsyidsavi
241 spdtvplgtg tsilskqvqn kpktgrsllg gpaalspltp sfgilpletp spgdgsylqn
301 ytntppvidv pstgapskks varigqtgtk svfsqsgnsr evtpilaqtq ssgpqtsttp
361 qvlsptitsp pnalprrssr lftsdssttk enskklkmkf ppkipnrktk sktnkggitq
421 pnindsleit kldssiiseg kistitpqiq afnlqkaaae glmsllremg kgylalcsyn
481 ckeainilsh lpshhyntgw vlcqigrayf elseymqaer ifsevrrien yrvegmeiys
541 ttlwhlqkdv alsvlskdlt dmdknspeaw caagncfslq rehdiaikff qraiqvdpny
601 ayaytllghe fvlteeldka lacfrnairv nprhynawyg lgmiyykqek fslaemhfqk
661 aldinpqssv llchigvvqh alkksekald tlnkaividp knplckfhra svlfanekyk
721 salqeleelk qivpkeslvy fligkvykkl gqthlalmnf swamdldpkg annqikeaid
781 krylpddeep itqeeqimgt desqessmtd addtqlhaae sdef
//
