LOCUS NP_003331 147 aa linear PRI 02-AUG-2023
DEFINITION ubiquitin-conjugating enzyme E2 D3 isoform 1 [Homo sapiens].
ACCESSION NP_003331
VERSION NP_003331.1
DBSOURCE REFSEQ: accession NM_003340.6
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 147)
AUTHORS Yalcin Z, Koot D, Bezstarosti K, Salas-Lloret D, Bleijerveld OB,
Boersma V, Falcone M, Gonzalez-Prieto R, Altelaar M, Demmers JAA
and Jacobs JJL.
TITLE Ubiquitinome Profiling Reveals in Vivo UBE2D3 Targets and
Implicates UBE2D3 in Protein Quality Control
JOURNAL Mol Cell Proteomics 22 (6), 100548 (2023)
PUBMED 37059365
REMARK GeneRIF: Ubiquitinome Profiling Reveals in Vivo UBE2D3 Targets and
Implicates UBE2D3 in Protein Quality Control.
REFERENCE 2 (residues 1 to 147)
AUTHORS Jiang C, Dai X, He S, Zhou H, Fang L, Guo J, Liu S, Zhang T, Pan W,
Yu H, Fu T, Li D, Inuzuka H, Wang P, Xiao J and Wei W.
TITLE Ring domains are essential for GATOR2-dependent mTORC1 activation
JOURNAL Mol Cell 83 (1), 74-89 (2023)
PUBMED 36528027
REFERENCE 3 (residues 1 to 147)
AUTHORS Ray P, Nancarrow DJ, Ferrer-Torres D, Wang Z, San Martinho M,
Hinton T, Wu JH, Wu A, Turgeon DK, Hammer MA, Dame MK, Lawrence TS,
O'Brien PJ, Spence JR, Beer DG and Ray D.
TITLE UBCH5 Family Members Differentially Impact Stabilization of Mutant
p53 via RNF128 Iso1 During Barrett's Progression to Esophageal
Adenocarcinoma
JOURNAL Cell Mol Gastroenterol Hepatol 13 (1), 129-149 (2022)
PUBMED 34416429
REMARK GeneRIF: UBCH5 Family Members Differentially Impact Stabilization
of Mutant p53 via RNF128 Iso1 During Barrett's Progression to
Esophageal Adenocarcinoma.
REFERENCE 4 (residues 1 to 147)
AUTHORS Haenig C, Atias N, Taylor AK, Mazza A, Schaefer MH, Russ J,
Riechers SP, Jain S, Coughlin M, Fontaine JF, Freibaum BD,
Brusendorf L, Zenkner M, Porras P, Stroedicke M, Schnoegl S,
Arnsburg K, Boeddrich A, Pigazzini L, Heutink P, Taylor JP,
Kirstein J, Andrade-Navarro MA, Sharan R and Wanker EE.
TITLE Interactome Mapping Provides a Network of Neurodegenerative Disease
Proteins and Uncovers Widespread Protein Aggregation in Affected
Brains
JOURNAL Cell Rep 32 (7), 108050 (2020)
PUBMED 32814053
REFERENCE 5 (residues 1 to 147)
AUTHORS Kanack A, Vittal V, Haver H, Keppel T, Gundry RL, Klevit RE and
Scaglione KM.
TITLE UbcH5 Interacts with Substrates to Participate in Lysine Selection
with the E3 Ubiquitin Ligase CHIP
JOURNAL Biochemistry 59 (22), 2078-2088 (2020)
PUBMED 32401531
REMARK GeneRIF: UbcH5 Interacts with Substrates to Participate in Lysine
Selection with the E3 Ubiquitin Ligase CHIP.
REFERENCE 6 (residues 1 to 147)
AUTHORS Ohta T, Michel JJ, Schottelius AJ and Xiong Y.
TITLE ROC1, a homolog of APC11, represents a family of cullin partners
with an associated ubiquitin ligase activity
JOURNAL Mol Cell 3 (4), 535-541 (1999)
PUBMED 10230407
REFERENCE 7 (residues 1 to 147)
AUTHORS Anan T, Nagata Y, Koga H, Honda Y, Yabuki N, Miyamoto C, Kuwano A,
Matsuda I, Endo F, Saya H and Nakao M.
TITLE Human ubiquitin-protein ligase Nedd4: expression, subcellular
localization and selective interaction with ubiquitin-conjugating
enzymes
JOURNAL Genes Cells 3 (11), 751-763 (1998)
PUBMED 9990509
REFERENCE 8 (residues 1 to 147)
AUTHORS Rogakou EP, Pilch DR, Orr AH, Ivanova VS and Bonner WM.
TITLE DNA double-stranded breaks induce histone H2AX phosphorylation on
serine 139
JOURNAL J Biol Chem 273 (10), 5858-5868 (1998)
PUBMED 9488723
REFERENCE 9 (residues 1 to 147)
AUTHORS Jensen JP, Bates PW, Yang M, Vierstra RD and Weissman AM.
TITLE Identification of a family of closely related human ubiquitin
conjugating enzymes
JOURNAL J Biol Chem 270 (51), 30408-30414 (1995)
PUBMED 8530467
REFERENCE 10 (residues 1 to 147)
AUTHORS Scheffner M, Huibregtse JM and Howley PM.
TITLE Identification of a human ubiquitin-conjugating enzyme that
mediates the E6-AP-dependent ubiquitination of p53
JOURNAL Proc Natl Acad Sci U S A 91 (19), 8797-8801 (1994)
PUBMED 8090726
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from BM750658.1, DA557036.1,
BC003395.1, AC018797.4 and BM967209.1.
Summary: The modification of proteins with ubiquitin is an
important cellular mechanism for targeting abnormal or short-lived
proteins for degradation. Ubiquitination involves at least three
classes of enzymes: ubiquitin-activating enzymes, or E1s,
ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
ligases, or E3s. This gene encodes a member of the E2
ubiquitin-conjugating enzyme family. This enzyme functions in the
ubiquitination of the tumor-suppressor protein p53, which is
induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan
2017].
Transcript Variant: This variant (1) encodes the predominant
isoform (1).
Sequence Note: This RefSeq record was created from transcript and
genomic sequence data to make the sequence consistent with the
reference genome assembly. The genomic coordinates used for the
transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803617.237277.1,
SRR1803611.223812.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA2142680, SAMEA2153733
[ECO:0000348]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..147
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="4"
/map="4q24"
Protein 1..147
/product="ubiquitin-conjugating enzyme E2 D3 isoform 1"
/EC_number="2.3.2.23"
/EC_number="2.3.2.24"
/note="ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog,
yeast); ubiquitin-conjugating enzyme E2-17 kDa 3;
ubiquitin-protein ligase D3; ubiquitin carrier protein D3;
ubiquitin-conjugating enzyme E2(17)KB 3; ubiquitin
conjugating enzyme E2D 3; E2 ubiquitin-conjugating enzyme
D3; (E3-independent) E2 ubiquitin-conjugating enzyme D3;
ubiquitin-conjugating enzyme E2D 3 (homologous to yeast
UBC4/5)"
/calculated_mol_wt=16556
Region 1..146
/region_name="UBCc"
/note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
domain. This is part of the ubiquitin-mediated protein
degradation pathway in which a thiol-ester linkage forms
between a conserved cysteine and the C-terminus of
ubiquitin and complexes with ubiquitin...; cl00154"
/db_xref="CDD:444715"
Site order(4,61..62,95..96)
/site_type="active"
/note="E3 interaction residues [active]"
/db_xref="CDD:238117"
Site order(72..73,77..78,80,84..86,88..89,93..94,97,102,105,
108,111..112,114,116..117)
/site_type="active"
/note="Ub thioester intermediate interaction residues
[active]"
/db_xref="CDD:238117"
Site 85
/site_type="active"
/note="active site cysteine"
/db_xref="CDD:238117"
CDS 1..147
/gene="UBE2D3"
/gene_synonym="E2(17)KB3; UBC4/5; UBCH5C"
/coded_by="NM_003340.6:486..929"
/note="isoform 1 is encoded by transcript variant 1"
/db_xref="CCDS:CCDS3660.1"
/db_xref="GeneID:7323"
/db_xref="HGNC:HGNC:12476"
/db_xref="MIM:602963"
ORIGIN
1 malkrinkel sdlardppaq csagpvgddm fhwqatimgp ndspyqggvf fltihfptdy
61 pfkppkvaft triyhpnins ngsicldilr sqwspaltis kvllsicsll cdpnpddplv
121 peiariyktd rdkynrisre wtqkyam
//
